LOCUS       DATGCJ010000001         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_45008, whole genome shotgun sequence.
ACCESSION   DATGCJ010000001 DATGCJ010000000
VERSION     DATGCJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45008"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VKN64_00005"
     CDS             <1..250
                     /locus_tag="VKN64_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMA58643.1"
                     /translation="VPSKKAWAEDLGLEKLKILSDFNPLGFAAKIMDVFIEEENISGR
                     VNILLNPEGEVVWVKEYEIHEAPDFDEVIEAIKEVQNK"
     gene            complement(392..973)
                     /locus_tag="VKN64_00010"
     CDS             complement(392..973)
                     /locus_tag="VKN64_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA58644.1"
                     /translation="MIVRFLGEASIEIEDDLNILIDPNFESEPETEPDIILVTHEHDD
                     HFDDKVVEQYPGAEIYGPQSFFDKFEVEGTVIERGETIKEDIEVLGCDCYGSEEAVCY
                     YYHGIYHTADSADYPKPEKDVKLMFTACFKDFFNEYLTLVKDIKPDIVIPYHFNSKDN
                     DAVQEAMELNKILREAGYDSRLLKPGDFIKFED"
     gene            1185..2102
                     /locus_tag="VKN64_00015"
     CDS             1185..2102
                     /locus_tag="VKN64_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA58645.1"
                     /translation="MKYDNTLLLSILLISSLIIMGVTAPTYAQEPLSLKIGIMPAVDS
                     APILLAEEKGYYQELNLDIKIDIYTNAVNRQSALQTDELDGAMTDLVAFVNNVNNGFP
                     IKITISTDGSFPFLVKEGFEEKDKIDIGMMEISVANFLSEQFLADQYELNKIFISAIP
                     ARLEMVKSGKLDMAIIPEPLASMGELAGLKKRVYENEYDFTPEAMIFTETALVKKSEA
                     ISRFHKAYNKAVREIQKDDTEAREVLIKRLNLKPEIKDLITMPVYHLARVPSRSYLNY
                     VIEWVEEKQEEEINLSYQNMVEEKFIQND"
BASE COUNT          787 a          324 c          385 g          709 t
ORIGIN      
        1 agttccaagt aaaaaagctt gggcagagga tttaggactt gaaaaattaa aaatactatc
       61 agactttaat ccactgggtt ttgcagctaa aataatggat gtctttattg aagaagaaaa
      121 catttcaggc agagtaaata ttcttctaaa ccccgaggga gaggttgtct gggttaaaga
      181 atatgaaatt catgaggcgc ctgattttga tgaagttatt gaagcaataa aagaagttca
      241 aaataaataa aaaatataag atattttgaa tataaattct tataaagttg aatctgctaa
      301 tattttttta aatattagaa gaaatatgaa ggatatgata aagacaaata ctgggaataa
      361 aaaagaaaac cgccgcgagg cggttttttc tttagtcctc aaatttgata aaatcacccg
      421 gttttaaaag tctggaatca taaccagctt ctcttaaaat tttattgagc tccatggctt
      481 cctggactgc atcattatct tttgaattaa agtgataggg aatcacaata tctggtttta
      541 tatctttaac cagagtcaaa tattcgttaa agaaatcttt aaaacaggct gtaaacatta
      601 gttttacatc cttttctggt ttcggataat cggctgaatc ggccgtatgg tagataccat
      661 ggtaataata acagacagct tcttccgatc cataacaatc acatcctaat acctcaatat
      721 cttccttaat ggtttctccc ctttctatta ctgtaccttc tacttcaaat ttatcaaaaa
      781 aggactgggg accatatatt tctgcccctg gatactgctc aactactttg tcatcaaaat
      841 gatcatcgtg ttcatgagta actaatatta tatcaggctc agtttcaggt tctgattcaa
      901 aattaggatc aattaagata tttaggtcat cttctatttc gatacttgct tcacctaaaa
      961 atctaactat catatcaatc cctccttata ttttattact tttatataaa tattaaaaac
     1021 ccttttttta agaatttaag agatatatat taatttaaaa gataaaatta ttctaatctg
     1081 cgttaataat ttgcttatag ttaggatatt aatatataat gtatattaat atagtaagga
     1141 ttaataatct caatctaaca accatatatg aggagttgga aaaaatgaaa tacgacaata
     1201 ctttattatt atcaatttta ttaattagtt cactaataat tatgggtgtt actgccccaa
     1261 cttatgccca ggaacctttg tcgcttaaaa tagggataat gccggctgtg gattcggccc
     1321 caatattatt ggcagaggaa aagggttatt accaagaatt aaatcttgat ataaagattg
     1381 atatttatac aaatgcagta aacaggcaga gcgctctgca gaccgatgag ctggacggag
     1441 caatgacaga tttagttgcc tttgtcaata atgtaaacaa tggttttccc attaaaataa
     1501 cgataagtac cgatggaagt tttccgtttt tagttaaaga agggtttgaa gaaaaagata
     1561 aaattgatat tggaatgatg gagataagtg tggcgaactt tctatcagaa caatttttag
     1621 ctgatcaata tgagttaaac aagattttta tttccgctat acccgctcgt ttagagatgg
     1681 tcaagtcggg taagttagat atggccataa ttccggaacc gcttgcttca atgggtgaat
     1741 tagccggctt gaaaaagcgg gtttatgaga atgaatatga ttttacaccg gaagcaatga
     1801 tctttactga aacagctctt gtaaaaaaat cagaagctat aagtcgtttt cataaagcat
     1861 ataataaggc tgttcgagaa atacaaaaag atgatactga agcccgtgaa gtattaataa
     1921 agagattgaa tcttaaacca gagataaaag atctgataac aatgcctgtc taccatctgg
     1981 caagagtacc tagtagatct tatttaaatt atgttattga atgggtagaa gaaaagcaag
     2041 aggaagagat aaacctttct tatcaaaata tggttgagga gaaatttatt caaaatgatt
     2101 aaggttaata atctaagctt ttcttatgaa aataaaaaag tagtaaaaga tgttaacctt
     2161 tctattcaga atggaaaaag ctgggcgatt attggtcctt ccggc
//
LOCUS       DATGCJ010000002         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_357738, whole genome shotgun sequence.
ACCESSION   DATGCJ010000002 DATGCJ010000000
VERSION     DATGCJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357738"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VKN64_00020"
     CDS             <1..504
                     /locus_tag="VKN64_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HMA58646.1"
                     /translation="PYKNQATFILGYRGMINLARRSGEIQTLYAHVVRENDEFSYQYG
                     LEPDLKHIPVEEERGNIKGAYMVAKFKDGGYYFEYMPIADILKRRDKSPAAKSEYSPW
                     NDMGIGYEEMVKKTVIRHAFKYLPVSIEIMRNVELTDETQKDLKEMNKDDFIDLEVED
                     ISVKNVS"
     gene            550..1458
                     /locus_tag="VKN64_00025"
     CDS             550..1458
                     /locus_tag="VKN64_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HMA58647.1"
                     /translation="MARATKRGLDYFPMDIQMEDKIKLVEAKYGIEGFGIIIRLYQKI
                     YANGYFIDWNEENVLLFSNEINIEITKINEIIEDSLNWQLFDRSLYENYFILTSHGIQ
                     VRYKEATYRRKQVKMYKQYNLLTGDEINVNVIYVDILTQNVNINRQNVNISTQSKVKK
                     SKVKKSKVNNIYTQEIEEIFNCWLELLSDINNPQLTKKQRELITTKLKKWDKDKIIDA
                     IQNYHEVLRSDYYYSHNFTFHNFIKQSNGVPRFIPGLDQKYDGDIWKDYQSISDRKNK
                     KTDMLKKLYLEAQLEDEQGEGGIDIL"
     gene            1455..2030
                     /locus_tag="VKN64_00030"
     CDS             1455..2030
                     /locus_tag="VKN64_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative helicase loader/inhibitor"
                     /protein_id="HMA58648.1"
                     /translation="MRKTEVVKLLKFLNSYYNAKFEYPRSSETDNKMLQETWLLFLKE
                     YDYSLVRTAIKKIVINNEWPPTPGEIIKEIESIKLPKEARLTAGEAWSLVLDIIGKYG
                     TSYNIKKAVDSLPIKVLKAARCVGGLQAIGKSSESDTYLMNSFLKAYKELDIRENKNN
                     LLPGSVRKEVQLLSDHFTNQSQSLLEDKGEK"
     gene            2030..2167
                     /locus_tag="VKN64_00035"
     CDS             2030..2167
                     /locus_tag="VKN64_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58649.1"
                     /translation="MSLSFNEKQILLTSLTKYESELNYDKDKIQALKNKIKKELSVDD
                     K"
     gene            2157..>2486
                     /gene="ssb"
                     /locus_tag="VKN64_00040"
     CDS             2157..>2486
                     /gene="ssb"
                     /locus_tag="VKN64_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMA58650.1"
                     /translation="MINNIVLIGRLVRDPELRYTGSGIPVGNFTLAVERDFKNQQGIK
                     EVDYIKIVTWRKLAEVIAAHLSKGRLVAVNGSLQINKSQDKDGRSYINPEVNADKVQF
                     LDWGSQTP"
BASE COUNT          908 a          374 c          554 g          650 t
ORIGIN      
        1 ccttataaaa accaggcaac ttttattctg ggatataggg gcatgatcaa cctggccagg
       61 cggtcgggcg agatccagac tctttatgcc catgtggtga gagaaaatga tgagttcagc
      121 tatcagtatg gtcttgaacc ggatctaaag catatcccgg ttgaagaaga acgtggcaat
      181 attaaaggag cctacatggt tgctaagttt aaagacggcg gctattactt tgaatacatg
      241 ccgattgcag acatcttaaa aaggcgggat aaaagcccgg ctgccaaatc agaatacagc
      301 ccctggaatg atatggggat tggctatgaa gagatggtca aaaaaactgt gattaggcac
      361 gcctttaaat acctgccggt tagcatagaa attatgagaa atgtagagct gaccgatgaa
      421 acccaaaagg atctaaaaga gatgaataaa gatgacttta ttgatttaga ggttgaagac
      481 atatcggtga aaaatgtttc atgataaaat acccggtctc aacggccggg ttctctttga
      541 ggtgatctaa tggcaagagc gacaaaaaga ggcctggatt acttccctat ggatatccag
      601 atggaagaca aaattaaatt ggttgaagct aaatatggta tagaaggctt tgggataatt
      661 atcaggctct atcaaaaaat atatgccaat ggttatttta ttgattggaa tgaagagaac
      721 gtccttcttt tcagtaatga aattaatatt gaaatcacga agataaatga aatcatcgag
      781 gacagtttaa actggcagtt atttgatagg agtttgtatg aaaattattt cattctaacc
      841 agccatggta tccaggttag gtataaagaa gcaacttatc ggagaaaaca ggtaaagatg
      901 tataagcagt ataatctcct aactggagat gaaattaacg tgaacgtgat ttatgtcgac
      961 attttaactc aaaatgttaa cattaatcgc caaaatgtca acataagtac acaaagtaaa
     1021 gtaaagaaga gtaaagtaaa gaagagtaaa gtaaataata tatacacgca agaaatcgaa
     1081 gaaattttta actgctggct ggaattgctt tccgatatca acaacccaca attaacaaag
     1141 aaacagcggg aactaattac taccaagttg aaaaaatggg ataaagataa gataattgac
     1201 gctattcaaa attatcatga ggttcttagg tcagactatt attattctca caactttact
     1261 tttcataact ttattaaaca gtccaacggg gtacccaggt ttataccggg gctggaccaa
     1321 aaatatgacg gtgatatctg gaaagattat caatctatta gtgatcggaa gaataaaaag
     1381 acagatatgt taaaaaaact ataccttgaa gctcagttag aagatgagca gggagaggga
     1441 ggaattgata tcctgtgaga aagactgagg tagtgaagtt gttaaaattc cttaattcct
     1501 attacaatgc taaatttgaa tatcctagaa gtagtgaaac tgacaataag atgctgcagg
     1561 agacctggct gttattttta aaagaatatg attattctct agtgaggacg gctatcaaga
     1621 aaattgtcat caataacgaa tggcctccga cacctggaga gattataaaa gaaatagaaa
     1681 gcattaaact gcctaaagaa gcaagattaa ctgcggggga agcctggtct ttggttttgg
     1741 atataatagg gaaatacggg accagttata atatcaaaaa agcagttgat tccctaccaa
     1801 taaaggtatt gaaggcagca aggtgtgtgg gaggcctgca ggcaataggg aaaagcagtg
     1861 agagtgatac ctatttgatg aattcatttt tgaaggccta taaagaactc gatatcagag
     1921 aaaataaaaa taacctattg ccgggatcgg tcaggaaaga agtccagctc ttaagtgatc
     1981 attttactaa tcagagccag tccttactgg aagataaggg ggaaaagtag tgagcttatc
     2041 attcaatgaa aaacaaattt tattgaccag cttaacaaag tatgaatctg agcttaacta
     2101 tgataaagat aagattcaag ctttaaaaaa taaaattaaa aaggagctat ccgtagatga
     2161 taaataatat agttttaata ggcaggttag tcagggatcc ggagcttaga tatacaggca
     2221 gtggtattcc agttggtaat ttcacattag cggtggagag agactttaaa aaccaacagg
     2281 ggataaaaga agtcgattat ataaaaatag taacatggag aaaacttgcc gaagtgattg
     2341 ctgcccatct tagcaagggt aggctggtgg cggtgaatgg ttcactccaa attaataaga
     2401 gccaggataa agatggcagg tcctatataa atccggaagt taatgctgat aaagtccagt
     2461 tccttgactg gggtagccag actcct
//
LOCUS       DATGCJ010000003         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_581202, whole genome shotgun sequence.
ACCESSION   DATGCJ010000003 DATGCJ010000000
VERSION     DATGCJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581202"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VKN64_00045"
     CDS             <1..345
                     /locus_tag="VKN64_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMA58651.1"
                     /translation="DMNFNELQEYINLKESQGREVLEEKVKLHQRLSIPFASFIFALL
                     AAPLGIKPGRSSGSAVGMGISIIVIFIYYIMMTIGDALGSQGTIAPWLGAWLSNIIFL
                     IVGSVLLYRVAR"
     gene            360..1634
                     /locus_tag="VKN64_00050"
     CDS             360..1634
                     /locus_tag="VKN64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HMA58652.1"
                     /translation="MSGINIMILVLIFSGIIAYIGDRIGMKAGKKRVSLFGLRPRYSS
                     IIITVMTGILIALLTISILLFTYTNLRQALFNINDVLDRLQNLNQKLEERDQELEQLK
                     NEIDQKTIELEELSQQRDQLTTELENTEQEYEQTRTDLNEAHQEIEQLKKTREELQKN
                     IALLEEEKSTLEEQVNELNQRISALTKDYEEVKELASQYRTGMVYYMGKNIVYQKGDI
                     VYSKVLEAGQSQDKIIEDLNKMLDEANEVAKKKDIKVDEQTGRALRVQYEDISNVAKI
                     LFNMDKGQKVIVSLVASFNVPEGDWLSVNFLLNRDFIVFNKGDVIASKVINASLTTTE
                     IEKELEGLLDTINGKAIQKGILPDSQGQVGSLDFSEFYTLLNRIKSIEGEVKVEVIAT
                     ENIWREDRLGDNIDFNIQSPIKGDINNNANGN"
     gene            1618..2016
                     /locus_tag="VKN64_00055"
     CDS             1618..2016
                     /locus_tag="VKN64_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HMA58653.1"
                     /translation="MLMGIDPGRNKCGIAIITEKGEEIKKEITETSNLSRKIEEVLSR
                     FSISKCILGNGTYADKVYNILQLLMDEEKIIFIEEEDSTYIAERRYLKENPPLGLNFL
                     NKIIKFKPKKPLDDYTAVVLVDKYLELEKK"
     gene            complement(2070..2576)
                     /locus_tag="VKN64_00060"
     CDS             complement(2070..2576)
                     /locus_tag="VKN64_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58654.1"
                     /translation="MMEKGIWTFGGSFDLGGNLSYTGEPTLEPDKVSTGFTVMAEYAM
                     PAKNSVSYGAGFAYQLSRSLEEFSEVSFSFTPIYGLLKYHMENQAYLVGHAGYNLLSG
                     SDAFKMSEGGAAKLGGGLYYAAGLGIGMENYTAEILYTVNNGTWETSEVKYDLSYSKF
                     SLAFGYQF"
     gene            2875..3747
                     /locus_tag="VKN64_00065"
     CDS             2875..3747
                     /locus_tag="VKN64_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58655.1"
                     /translation="MKKVLIFMLMLVMVLSFSLVGAAYYDETTNHYDGDAFNGVQEDA
                     DIIDTGNVLEYSLQVSENPSRHLIEGIYFFNEDSYLSFENWIDPNDSSFFNDSEAKLG
                     YEFYDKNGWILSTELAGYVPGDYTGTLRITKVVNDKLSLNNNLSIKLEENFNRNLRTG
                     LTYKLNEKNNFKISIGRPSYSYVSNFSGLFEDLCLRAALETKINEKFNNVIFIRKHFN
                     NDNIRIIEQIQYVGSDDFYFDTAFELNTNYSNQFEFESKKALSDNFYLTFDLYKNFGG
                     NEGYWAYPGIQYEF"
     gene            4036..4524
                     /locus_tag="VKN64_00070"
     CDS             4036..4524
                     /locus_tag="VKN64_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58656.1"
                     /translation="MKKGYLSLFLILVFVLLLTSGTAFAQEQEEGKYKIGFQASFPSY
                     GLSGIMRVNEKVSVQGVAAPFGDVTTFAVRGLYRFREEPGWNAYGYGSVGMYNYTAGT
                     IDESKTGFGAGVGIEYDWQMRFVEGAPPLTSNLEIGIGSVNFDYYDYSTFSFGVGTHY
                     KF"
     gene            4702..>4975
                     /locus_tag="VKN64_00075"
     CDS             4702..>4975
                     /locus_tag="VKN64_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA58657.1"
                     /translation="MAVTLKDIAEKVGVSESTVSRVLNGIPKASKKTREEIFRVAREL
                     NYTPNEVARSLVTKKTNTIGLIISDLSNLYFSMVADGIESVVAQNDY"
BASE COUNT         1800 a          677 c          913 g         1585 t
ORIGIN      
        1 gacatgaatt ttaatgagtt acaggagtac attaatctca aagaaagtca aggacgcgaa
       61 gttttagaag aaaaggtaaa acttcatcaa aggttatcta ttccttttgc tagttttatt
      121 ttcgccctac tggcggctcc tctcggtata aaaccgggta gatcgagcgg ttcagccgtg
      181 ggaatgggaa taagtataat agtaatcttt atatattata taatgatgac aattggagat
      241 gctttaggtt cacaaggtac tattgctccc tggctcggag cctggttgtc aaatataatt
      301 tttctaatag taggtagtgt tttattatat agagttgcta gataacagga gtgtaaatta
      361 tgagcggaat taatataatg attttagttt taatattcag cggtataatc gcctatattg
      421 gtgataggat tggaatgaaa gccgggaaga aaagggtttc tctttttggc cttagacccc
      481 gctattcttc cataattatt acagttatga cgggaatttt gattgccctc ttaactattt
      541 ctatattact atttacctac accaatttaa gacaggcctt atttaatata aatgatgtac
      601 tggaccgtct ccaaaattta aatcaaaaat tagaggaaag agaccaagaa ttagaacaac
      661 ttaaaaacga aatcgaccag aaaacaattg agctagagga actttcacag cagcgtgatc
      721 aactcacgac ggagcttgaa aatactgaac aggaatacga acaaactaga actgatttaa
      781 atgaggccca tcaagagatt gaacaattga aaaagacaag agaagaactg cagaaaaata
      841 tcgccttact agaggaagaa aaaagcacgt tagaagaaca ggtaaatgaa cttaaccaaa
      901 ggattagtgc ccttactaaa gactatgaag aagttaaaga acttgcttct caatacagaa
      961 ccggtatggt ctattacatg ggtaagaata ttgtttatca gaaaggtgat atagtatatt
     1021 ccaaagtatt agaagccggt caatctcaag ataagataat agaagatcta aataaaatgt
     1081 tagatgaagc caatgaagtt gctaaaaaga aggatataaa agttgatgaa caaaccggac
     1141 gcgctttaag agtacagtat gaagatatat cgaatgtagc caagatatta tttaatatgg
     1201 ataaaggaca aaaagttatt gtaagtttag ttgcaagttt caatgttccc gaaggtgatt
     1261 ggttatcggt taattttctt ttaaacagag actttatagt atttaataaa ggtgatgtga
     1321 tagccagcaa agttataaat gcaagcctta ccaccacaga aatagaaaaa gagttagagg
     1381 gactattaga tacaataaat ggaaaagcaa tccagaaagg catcttacct gattcacaag
     1441 gccaggtggg ttcacttgat tttagtgaat tttatacact gcttaataga attaaatcaa
     1501 tcgaagggga agtaaaggta gaagtaattg ctactgaaaa tatttggcga gaagatagat
     1561 tgggtgataa tatagacttt aatattcaaa gccctataaa aggagatatt aacaataatg
     1621 ctaatgggaa ttgatccggg acgtaataaa tgcgggatag ctattattac cgaaaaaggt
     1681 gaagaaatca aaaaggaaat tacagaaacc tcaaatttaa gccgcaagat agaagaagta
     1741 ctaagtagat tttcaataag taagtgtatc ttaggtaatg gcacctatgc agataaggta
     1801 tataatatct tacaactttt aatggatgaa gaaaaaatta tatttataga agaagaggat
     1861 tccacctata ttgcggaacg aagatattta aaggaaaatc cacctttagg tttaaacttt
     1921 ttaaataaaa tcattaagtt taaacctaaa aaaccactgg atgattatac tgcagtggtc
     1981 ctcgtcgata aatatttaga actagaaaaa aagtaataga actaaaaaca accctcacca
     2041 attaaattga tgagggttta gtttttttac tagaactgat aaccaaaggc gagactgaat
     2101 tttgaatagc ttaaatcata tttaacttcg cttgtttccc aggtaccgtt atttacagta
     2161 taaagaattt cagcagtata attttccatt ccaataccta gacccgctgc ataatagagt
     2221 ccgccaccta atttagctgc accaccttca ctcattttga atgcatcact accacttaag
     2281 agattataac cagcatgtcc tactagatat gcttgatttt ccatgtgata tttcaaaagg
     2341 ccataaatag gtgtgaaact aaaactaact tctgaaaatt cttctaaact ccgagatagt
     2401 tgataagcga atcccgcgcc ataactaaca ctatttttag cgggcattgc atactcagcc
     2461 ataacagtaa aaccagtaga aactttgtct ggttcaagag ttggttcacc tgtgtaagag
     2521 agatttccac ctaagtcaaa gcttccccca aaagtccaaa tacccttttc catcatatgc
     2581 tcagaagcta cagcactgcc actaaataat aatagagata agacaagaag aataacacta
     2641 atttttttca tctaaatcct ccttttaaat tttttgtatt tttttactat atacttataa
     2701 ttattgattg atagaaataa tccttcttta tttacgtgtt tatgattaat ttttttaaga
     2761 ggcggtaaaa aatttttaat aaatttggta aattaaggag gaaaagggaa ataaatcagc
     2821 aataaataaa ttaatatact aaaatattta tattacacga ggaggatata agagatgaag
     2881 aaagttctaa ttttcatgtt gatgttggtt atggttttat catttagctt agttggtgct
     2941 gcttattacg atgaaacaac aaatcattat gatggagatg cgttcaatgg agtacaggaa
     3001 gatgctgata ttattgatac cggtaatgta ttagaatatt cattgcaggt aagtgaaaat
     3061 ccatctagac atttaataga gggaatctat tttttcaatg aggatagtta tctaagtttt
     3121 gaaaattgga tagatcctaa tgatagttcc ttttttaatg attcagaagc caaattaggt
     3181 tatgaatttt atgataaaaa tggttggata ttaagtactg aacttgcagg ctatgtccca
     3241 ggtgattata ccggtacttt aagaattact aaagttgtta atgataaact ttccttaaat
     3301 aataatttat caattaaatt agaggaaaat tttaatagaa atttaagaac cgggttaaca
     3361 tataaattaa atgagaaaaa caacttcaaa attagtattg gtagacccag ttatagttat
     3421 gtaagtaatt ttagcggatt atttgaagat ctctgtttaa gagcagcctt agaaactaaa
     3481 attaatgaga aatttaataa tgtaatattc attagaaaac attttaataa tgataatatt
     3541 agaattatag agcaaattca atatgttggt tctgatgatt tttattttga tactgctttt
     3601 gaacttaaca caaattattc taatcaattt gaatttgaaa gtaaaaaagc tttatctgat
     3661 aacttttatt tgacatttga tttatataag aactttggtg gaaacgaagg atattgggca
     3721 tatcctggta tacaatatga attttaatta atataaaaga gattcccatg gtctagccga
     3781 taggccatgg gaatttattg ttttataata tttctacgta taaggcttta tatgagatta
     3841 ctttagttaa tattaatgtt ctattattta ttgctctaaa attattttaa ttatccaatt
     3901 attacattaa ttggctttat ttcatatata tgtttgaaat ttataaatat gaatcatata
     3961 atgaaattag gtagctacat ataaggttat attagtaagg tagttatcaa taataaaaca
     4021 aaggagagat attttatgaa aaaaggttat ctttcactat ttttaatctt agtctttgtt
     4081 ttgttattga catcaggaac tgcttttgca caggaacaag aggaaggcaa gtataagatt
     4141 ggtttccagg cttcttttcc tagttatgga ttaagcggga tcatgagagt taatgaaaaa
     4201 gtttctgtac agggggtagc tgctcctttt ggagacgtta caacatttgc tgtgagaggc
     4261 ctatataggt tcagagagga accaggttgg aatgcatatg gttatggttc agttggtatg
     4321 tacaattaca ctgcaggtac tattgatgaa agtaagactg gttttggggc cggagttggt
     4381 atcgaatatg actggcagat gagatttgtt gaaggggctc cacctctaac cagcaatctc
     4441 gaaattggta ttggtagtgt taattttgat tactatgatt attctacttt ttccttcgga
     4501 gtaggaactc attataagtt ttagtcttaa aaattaagaa atactctaaa gaaggggcct
     4561 gtttgaacac aggtcccttt ttgaatgttt aaaactatta acataattat ctgaaaatta
     4621 ggcttgacat tataagattt ttttatatat aataataagt agaaaggaca aacgtttgcc
     4681 caaaagaaga ggagaattca tatggcggtt actttaaaag atattgcgga aaaagtaggt
     4741 gtatctgaat cgacagtttc tcgcgtatta aatggtatac cgaaagccag taaaaaaaca
     4801 cgggaagaaa tattcagagt agctcgcgaa ttaaactata ctcctaatga agtagccaga
     4861 tctcttgtta ccaagaagac aaatacgatt gggttaatca tcagtgacct ctcaaatctc
     4921 tacttttcta tggttgctga tggtattgaa agcgttgtag cccaaaatga ttaca
//
LOCUS       DATGCJ010000004         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_402527, whole genome shotgun sequence.
ACCESSION   DATGCJ010000004 DATGCJ010000000
VERSION     DATGCJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402527"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VKN64_00080"
     CDS             complement(<1..622)
                     /locus_tag="VKN64_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMA58658.1"
                     /translation="MDMSNKMDKKLDFGSKKYLQLGKLLGQIDTFKGSWQEIEHKEAR
                     YLKELRQLATVASVGSSTRIEGVKMTDAEIEKLLRSVKFNKLEKRDEQEVVGYYDALQ
                     VILDHYQDMELTERYIHQLHGILLKHSSKDQNHKGRYKQLSNKVVANYPDGTQRLIFN
                     TKEPHLTQSEIQQLLVWTNGAITSEEIHPLLIVGSFVYEFLSIHPYQ"
     gene            complement(796..1149)
                     /locus_tag="VKN64_00085"
     CDS             complement(796..1149)
                     /locus_tag="VKN64_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58659.1"
                     /translation="MIILSRGYYVSSGKVYEEWHAGIYMNYKIFSAKLFQGDNSVLVA
                     SIKTNVEVEEYSFKIGDFVISNNQSRFNIENSNLCTYREDGSLYQVFSIFNKYRLIDE
                     FGVTFEYRVFESEEE"
     gene            complement(1146..1556)
                     /locus_tag="VKN64_00090"
     CDS             complement(1146..1556)
                     /locus_tag="VKN64_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58660.1"
                     /translation="MKTVVEELIGNLKTQDFGAKTGGFNVLNVSDNLYAPATFWNTYN
                     KPWLQTAINRGDEIAAASNPLDINNLFKGLNGIPQSELNILNGLSTPEQFASYFKNLN
                     DPDIYSQLSGFGQEFELLSKSNYVLDLGTKKFIK"
     gene            complement(1595..>2302)
                     /locus_tag="VKN64_00095"
     CDS             complement(1595..>2302)
                     /locus_tag="VKN64_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58661.1"
                     /translation="ANYLDDFVTVLGSTPTKPAALAADLDPKTAARIAKGTELSDGFI
                     SVFKNDWGIPSPQFDQLKLDLAAGGIPLKKFFADEGESGLKAWEVCFKDVAKRVDIPW
                     LTKLTESSGISKIDNANPLNVSWSNNIIGQNSSVSALDFWNDVSKNFLHYEQGDFIFK
                     YDYNSGKLLLGNKASNKIIGFYEGLENVPIGVYELSPSNIVQKLKRYHGESGPSSPIL
                     ICLTIIKSHQLQERQQP"
BASE COUNT          671 a          465 c          373 g          793 t
ORIGIN      
        1 cttggtaggg gtggatcgac aaaaactcgt acacaaagga tccaacgata agtagtggat
       61 ggatttcttc ggatgtaatg gccccattgg tccatactaa aagttgttgg atttcgctct
      121 gggttaggtg tggttctttt gtattaaata taagcctttg agtcccatcc ggataattcg
      181 ctactacttt attcgatagc tgcttatacc gtcctttatg attttgatct ttgctactat
      241 gcttcaataa tattccatgc aattgatgga tgtaccgttc agtcaattcc atgtcttgat
      301 aatgatctaa aatcacttgt agagcatcat aatagcccac cacttcttgt tcatctctct
      361 tttcaagctt attgaatttt accgacctca acagtttttc gatttctgca tctgtcatct
      421 tcactccttc aattcttgtc gatgagccca cactagccac ggtggccaat tgacggagct
      481 cctttaagta tcttgcctcc ttgtgctcta tttcctgcca cgatcctttg aaggtgtcta
      541 tctggcctaa taacttacca agctgcaagt attttttact tccaaagtca agctttttat
      601 ccattttatt agacatatcc attttgtatc tatttctatc taatagcaaa tataatgaaa
      661 catatctatt ttgtatccct ttgtatccat tttgtctatt tgtaaatgcc gtgcatcagg
      721 acaggattct ttatatgttc gatctgatgc ttaaagatgc tcagactaat tcgctgcaag
      781 aaaaattggc ataagctact cttcctcact ttcaaaaact cgatactcaa atgtcactcc
      841 aaattcatct attaatctat atttgttgaa tattgaaaac acctgataaa gtgagccatc
      901 ttctctatat gtacacaaat tagagttttc aatgttaaag cgactttgat tgttagatat
      961 gacaaagtct ccaattttaa aagagtactc ctcaacttcg acatttgttt ttatagatgc
     1021 aactaaaaca ctattatctc cttgaaatag ttttgcactg aaaatcttat aattcatgta
     1081 aattccagca tgccattctt catacacttt tcctgaagaa acataataac ctcttgacaa
     1141 tattatcatt taataaactt tttagtccct aagtcaagaa cataattact ctttgatagt
     1201 agctcaaact cttgtccaaa accagacaat tgcgaataaa tatcaggatc gttcaaattc
     1261 ttaaaatagg atgcaaactg ctcaggggtt gacaatccgt ttaatatatt taactctgat
     1321 tgtggaatac cattaagtcc tttaaataag ttatttatat ctaacggatt actagcagct
     1381 gcaatttcat ctcctctgtt aatagctgtt tgtaaccaag gtttattata tgtattccaa
     1441 aatgttgcag gggcatacaa gttatccgaa acattcaata cattaaaccc acctgtcttg
     1501 gctccgaaat cctgtgtttt gaggtttcct attaattcct ctacaacagt tttcatatca
     1561 ccagagaaat aaggatagta ggtgaattta cctactatgg ttgttgtctt tcctggagtt
     1621 gatgcgattt gattattgta aggcaaatta aaattggaga gcttgggcca ctttccccat
     1681 gatatctttt aagtttttga actatattag aaggactaag ttcataaact ccaataggta
     1741 cattttcaag tccttcgtag aatcctataa ttttatttga tgctttatta ccaagtaaaa
     1801 gtttcccact attatagtca tacttaaaaa taaaatctcc ttgttcataa tgtaagaagt
     1861 tctttgatac atcattccaa aaatcaagtg cagaaacact tgaattctga ccaattatat
     1921 tattactcca tgatacatta agaggattcg cattgtcaat tttcgaaata cctgaagact
     1981 ccgtaagctt agttaaccat ggaatatcaa ctcttttggc tacatcttta aagcaaacct
     2041 cccacgcctt aagcccactt tcaccttcat cagcaaaaaa cttttttaat ggaattcctc
     2101 cggcagcaag atcgagcttt agttggtcaa actggggtga ggggatgccc caatcgttct
     2161 taaaaactga aatgaatccg tcgcttaact ctgtgccttt tgctatcctc gccgctgtct
     2221 tcgggtccag atcggcggcg agggcggcgg gttttgtggg ggtggagcca aggactgtga
     2281 caaagtcatc gagatagttg gc
//
LOCUS       DATGCJ010000005         4557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_492140, whole genome shotgun sequence.
ACCESSION   DATGCJ010000005 DATGCJ010000000
VERSION     DATGCJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492140"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..639
                     /locus_tag="VKN64_00100"
     CDS             37..639
                     /locus_tag="VKN64_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58662.1"
                     /translation="MQRPYIILNKCDNTPGCPVIEACPQDIFHYDYNVKKLLLNDNKC
                     LECSLCVTECDHDAVRMYEPEIIKEKRKQEDQYGMALAERIKDHYNIEPTSIYNDEQI
                     TAIKDFEKIKKYKNVLLYVYGNWQAESYIGYSFFKEIIRELEEEIDNFKGFKISYKEL
                     DEIEITELPSHLIIKDGKVKNKYSGIKNPMYFVPTLISEY"
     gene            761..1402
                     /locus_tag="VKN64_00105"
     CDS             761..1402
                     /locus_tag="VKN64_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HMA58663.1"
                     /translation="MQKIEMENWPRKQHFDFFIDFDYPQFNITVDIDITRLINYIREK
                     DISFFKSIVFLVTKTANQVEEFKYRIIDNNQVVIVDKVNPSFTIMGENDVFSFCNSTY
                     YDNMDDFFASMEKNIEKYKENPSVSAEENQLDAVYLTSLPWLSFNSITHPIDIKNVDS
                     NPRIAWGKYYKEDGKIILPFSVQVHHALMDGYHVSKYINKFKEKINNPQKSLK"
     gene            1415..1876
                     /locus_tag="VKN64_00110"
     CDS             1415..1876
                     /locus_tag="VKN64_00110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA58664.1"
                     /translation="MEIKNLKDNQQYLEEVNHWLYSQWGHHDEDNDEKDWLKSKKEKL
                     NNNQSLPIRFVALEDGQPVGTASIVKHDMKTHTELEPWMADVYVKEEKRNQGIGSKLV
                     NKIIEEAQQQKFSTLYLFTPDKKSFYEGLGWNSFFNEEYRGENVDIMVYNL"
     gene            1893..2717
                     /locus_tag="VKN64_00115"
     CDS             1893..2717
                     /locus_tag="VKN64_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA58665.1"
                     /translation="MKEKRVDLHIHTNISDGSWDVEKLLEVIKRKKINIFSITDHDDL
                     KNVIKMSQQELPDDIKFLKGVEVSTTLDRKEFHLAAYNINLEDDQLKELLKKNRKIRK
                     EFNKSIIKYFEKTNNLELMNEYYDYKHDNNRGGWKALNFLIDKRLVNDLEDFFIKISN
                     MSEEMVFLHPEKAIKVIHQAGGFAFLAHPASYYNGELLTRDFLKEWVALGIDGIEAYS
                     PYLNQLKDAQYYIDFCKENDLMYSTGSDCHGAFIKDRKVGKPEVYLKDINIDKLLK"
     gene            2731..3096
                     /locus_tag="VKN64_00120"
     CDS             2731..3096
                     /locus_tag="VKN64_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HMA58666.1"
                     /translation="MSLLGNIIWIIFGGLIEAIGWVLAGILLSITIIGLPFGIQCFKI
                     AKVQLAPFGKEIVNANDSGTNLIMNIIWIILFGWEIALMNLISALIFAITIIGIPFAK
                     QNLKLAKVSLFPFGKKIIR"
     gene            3114..3872
                     /locus_tag="VKN64_00125"
     CDS             3114..3872
                     /locus_tag="VKN64_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HMA58667.1"
                     /translation="MEKEYQKLREFLKSTEWVRRDELKTPKDEGRKRPDAVKDYSKEA
                     EIISLPAIDQIETPENSLKELLNKRRSRRKYNTSSLSIDELSYLLWATQGLSEDNIRL
                     RTVPSGGAVHPFETYLYISRVQDMKKGLYRYLPDKHSLILLKEIENGKRDIIAAANGQ
                     KFVGEAAVVFLWTVIPKRGEWKYGILSHKLAALDAGHLCENLYLAVEDIKAGMCAVDA
                     YDQKTADKFLKVDGESEFVIYMATVGKVDESERK"
     gene            3853..4398
                     /locus_tag="VKN64_00130"
     CDS             3853..4398
                     /locus_tag="VKN64_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA58668.1"
                     /translation="MRAKGNKMIATDRLILRKFNKEDINDFHEIFSQTEVVKYLPIEP
                     LTYKEAVDKLDNIINNLSDLDKEKPKFILAVQLKENNKVIGWVGSGPLPFAEEKIELF
                     YALHSVYWKKGFAFEAASGLLNFLRDEYDIKPIYALVDDNNRGSVKILKKLNFTFEKK
                     VEIEKDKYRFFNGLDLYKLKE"
BASE COUNT         1716 a          540 c          864 g         1437 t
ORIGIN      
        1 tttgtaaata tagattaaat ctagtaggtg atttatttgc agagacctta tatcatttta
       61 aacaaatgtg ataacacacc gggatgtcct gtgattgaag cttgtccaca ggatattttt
      121 cattatgatt acaatgtaaa aaaactgctt ttgaatgata ataaatgtct ggagtgtagt
      181 ttgtgtgtaa cagaatgtga tcatgatgct gttagaatgt atgaacccga aataataaaa
      241 gaaaaaagaa aacaagaaga tcaatatgga atggcattag cggaaaggat taaagatcat
      301 tataatattg aacctacttc tatatataat gatgaacaga ttacagccat aaaagatttc
      361 gaaaagatca agaaatataa aaatgtatta ctttatgtat atggaaactg gcaggccgaa
      421 agttatattg gctattcatt ttttaaagag ataattcgag aattagaaga agaaatagat
      481 aattttaaag gatttaaaat tagttacaaa gaattagatg aaatagaaat tacggaacta
      541 cctagccatt tgattataaa ggatggtaaa gttaaaaaca aatatagtgg tataaaaaat
      601 ccgatgtatt ttgtacctac tttaatatct gaatactaat attaagcccc atggataaaa
      661 gttcatgggt tttttaaatt taaaaaagga aaagttcttt aataaaagaa ttagtattaa
      721 atagatactt taaaatgttg ataatttgga gtgttgataa atgcagaaaa tcgagatgga
      781 aaactggcct agaaagcagc attttgactt ctttattgat tttgattacc ctcagtttaa
      841 tattactgtc gatatagata ttactagact gataaattac ataagagaaa aagatatctc
      901 attttttaaa agtattgtct ttttggttac gaaaactgcc aaccaggttg aggaatttaa
      961 atacagaatt attgataata accaggttgt aattgtagat aaagtaaatc cctcttttac
     1021 aattatgggt gaaaatgatg tatttagttt ttgtaattct acttactatg ataatatgga
     1081 cgattttttt gcttctatgg aaaaaaacat tgaaaaatat aaggaaaatc cgagtgtatc
     1141 agcagaggaa aatcaactgg atgcagtcta tttgaccagt ttaccttggc ttagttttaa
     1201 cagtatcact catcctattg atattaaaaa tgtagattca aatcccagaa ttgcctgggg
     1261 taagtactat aaagaagatg gtaagattat tttacccttt tctgtccaag ttcaccatgc
     1321 tttaatggat ggttatcatg tttctaagta tataaataaa ttcaaggaaa agatcaataa
     1381 ccctcaaaaa tctttaaaat aaagggtggt aaaagtggaa attaaaaatt taaaagataa
     1441 tcagcagtat ttagaggaag ttaatcactg gctgtatagt cagtgggggc atcatgatga
     1501 agataatgat gaaaaggact ggttaaagtc taaaaaagag aagttaaata ataatcaatc
     1561 gttaccgatt agatttgttg cattagagga tggacaaccg gtaggaacag cctcaatagt
     1621 aaagcatgat atgaaaactc atacggagtt agaaccctgg atggcagatg tttatgtaaa
     1681 agaagaaaaa agaaaccagg gtattggttc taagcttgtc aataagataa ttgaggaggc
     1741 ccaacagcaa aagtttagta ccttatattt gtttacccct gataaaaaat cattttatga
     1801 aggactgggc tggaattctt ttttcaatga agaatatagg ggagaaaatg ttgatataat
     1861 ggtatataat ctctaaagaa gggaatttta cattgaaaga aaaaagagtt gatcttcata
     1921 ttcatacaaa tatttctgat ggctcctggg atgtagaaaa attattagag gtaatcaaga
     1981 gaaaaaagat taatattttt tctatcacag atcatgacga tttaaaaaat gtaatcaaaa
     2041 tgtcccagca agaattacca gatgatatta agtttcttaa aggagtggaa gtttctacta
     2101 ctttagatag gaaagaattt catcttgctg catataatat taatttagaa gatgatcaac
     2161 tgaaggaact attaaaaaag aatagaaaga taaggaaaga atttaataaa agtattatta
     2221 aatattttga gaaaacaaat aacttagaat taatgaatga atattatgat tataaacatg
     2281 ataataatag agggggctgg aaagctttaa actttcttat cgataaaaga ttagtaaatg
     2341 atttagagga cttttttata aaaatatcaa atatgagtga agagatggtt tttttgcatc
     2401 ctgaaaaagc aattaaagtt attcatcaag cgggggggtt tgccttttta gcacatcctg
     2461 cttcctatta taatggggaa cttctcacac gtgatttttt aaaagaatgg gttgcgctgg
     2521 gtatcgatgg aatagaagca tattctcctt atctaaatca actcaaggat gctcaatact
     2581 atatagattt ttgtaaagaa aatgatttga tgtattcaac tggttcggac tgtcatggag
     2641 cttttataaa agatagaaaa gtaggtaaac cagaagtata tctaaaagat attaatatag
     2701 ataaattatt aaagtgatga ggtgatcaaa ttgtcattac tagggaatat tatctggatt
     2761 atatttgggg gccttattga agcaattggc tgggttttag ccggtatatt attatctata
     2821 actattatcg gtcttccctt tggaatacaa tgttttaaaa tagcaaaagt acagttagcc
     2881 ccttttggta aagagattgt taatgccaat gacagcggca ccaatctcat tatgaatata
     2941 atctggatta ttttattcgg ctgggagata gctttaatga acttaattag tgctttaata
     3001 tttgctatta caataatagg aatacctttt gccaaacaaa atttaaaatt agccaaagta
     3061 tcactttttc ccttcggcaa aaaaataata agataatcac gaggtgttaa ttcatggaaa
     3121 aagaatatca aaagttacgc gaatttttaa aatcgacaga atgggtaaga agagatgaac
     3181 tgaaaacccc caaagatgaa ggcagaaaga gaccagatgc tgttaaagat tacagtaaag
     3241 aagctgaaat aataagttta ccggctattg atcagattga gactccagaa aattctttaa
     3301 aagagctgct taataaaagg agaagtcgga gaaagtataa tacgagttct ttgagtattg
     3361 atgaactttc ctatttatta tgggcgaccc agggtctatc agaagacaat attagattaa
     3421 gaacagttcc ttcgggagga gcagttcatc cctttgaaac atatctttat ataagcaggg
     3481 tacaggatat gaaaaagggt ttataccgct atttacctga taaacattct ttgattttgc
     3541 ttaaagaaat agaaaacggg aaaagagata tcattgcagc tgccaatggt caaaagttcg
     3601 taggagaggc agcggtagta tttttatgga cagttatacc aaaaagagga gaatggaagt
     3661 acggtatttt atcccataaa ctggctgctt tagatgccgg tcatctctgc gaaaacctct
     3721 atctggctgt agaagatatt aaggcgggca tgtgtgcagt ggatgcctat gaccaaaaaa
     3781 cagctgataa atttttaaag gtagatggcg agtcagaatt cgttatttac atggcaacag
     3841 tggggaaggt agatgagagc gaaaggaaat aaaatgatag ccacagatag attaattcta
     3901 agaaagttta ataaagagga tattaatgat tttcatgaga tcttttcaca aaccgaagtt
     3961 gtaaaatatt taccaattga gcctttaact tacaaagaag cagttgataa gttagataat
     4021 attattaata atctaagtga cttagataaa gaaaagccta aatttatatt ggccgttcag
     4081 ttaaaagaga ataataaagt aatcggctgg gtgggtagtg gtccccttcc ttttgctgag
     4141 gaaaaaattg aattatttta tgctttacat tcagtttatt ggaagaaggg ctttgctttt
     4201 gaagcagctt ccggcctatt aaatttttta agagatgagt atgatattaa accgatatat
     4261 gcccttgtag atgataataa caggggttct gttaagatat taaaaaaatt gaattttact
     4321 tttgaaaaga aagttgagat agaaaaagat aagtatagat tttttaatgg tctagattta
     4381 tacaaattaa aagaataaat tttaggaggt gaatttgatg gataatacaa ataagaggag
     4441 taataccttc ctaaaagcac ttaatctatt ttcttttttc ttagttatag ttgttaattt
     4501 tctagctaat taccttccta tcaatggtta taatacgggc gaactttcag atatgta
//
LOCUS       DATGCJ010000006        15924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_179512, whole genome shotgun sequence.
ACCESSION   DATGCJ010000006 DATGCJ010000000
VERSION     DATGCJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179512"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VKN64_00135"
     CDS             complement(<1..998)
                     /locus_tag="VKN64_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMA58669.1"
                     /translation="MDKAKEIIAKLNEQQEKAVKLHKGPGLIIAGAGSGKTRVLTNRI
                     AYLIEKYEVKPYQILGLTFTNKAADEMKERVQKMTGRDADDIFLGTFHSFCVQILRQE
                     INRLEFNRYFTIYDSYDQKKMVKNILKDLNYDVKKFRPSAVLNRISNAKNELIEAAAF
                     QKDAIGYFEKIVAAVYEEYEKRLKNNNALDFDDLIMKTVELFLDYPEVLNRYQERYRY
                     ILIDEYQDVNFAQYKFAQLLASKYRNIFVVGDPDQSIYAFRGADIRNILNFEEDYPDA
                     KVIKLEQNYRSHKKILDVADNVIENNKNRVPKRLKAARGEGGKVYYKALSSAEKEA"
     gene            complement(1024..1437)
                     /locus_tag="VKN64_00140"
     CDS             complement(1024..1437)
                     /locus_tag="VKN64_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMA58670.1"
                     /translation="MEAENSGIYIVKIKLTKNQSIKVGALGENNFKKGYYYYIGSAQK
                     NLESRIERHLRTKKKFHWHIDYLLDKAKIDDYFTLNGSKDYECKLFNYLYHKDQFKLM
                     VEGFGSSDCSCISHLLYSKNDLNLKNMLEDFKKDI"
     gene            1575..1651
                     /locus_tag="VKN64_00145"
     tRNA            1575..1651
                     /locus_tag="VKN64_00145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1609..1611,aa:Pro,seq:cgg)
     gene            complement(1810..2256)
                     /locus_tag="VKN64_00150"
     CDS             complement(1810..2256)
                     /locus_tag="VKN64_00150"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2125..2127),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMA58671.1"
                     /translation="MLKVGDSLPDFCLPDQDGKEHCRDDFKDQRLVLFFYIKDNTPGU
                     KKESVAFQRQLKTLEGLNTKVVGVSKDSVESHKNFAEEYDLTYPLLADTDKKLAKKFD
                     VVNLVGIYKRSTFIISKNQKIMKVYPKVKVDGHAERVVEFLKELKE"
     gene            2579..4147
                     /locus_tag="VKN64_00155"
     CDS             2579..4147
                     /locus_tag="VKN64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA58672.1"
                     /translation="MSRFNITITILLVLVLTLSFTVSAEEGTYGGTLVYGRGNDSVSL
                     DPINTTDGESFKVTQQIYDTLLMYKPGTTEPVPCLAKEWEVTEDGKKWTFYLREDVNF
                     HDGNPFNAEAVKFNFDRWRFEDNPYHVGGTFASYPYVFGGFPGIIKEVNVVSDYVVEF
                     VLETPQAPFLANMAMVSFAIASPEAVKEWGENYFKHPVGTGPFEFVEWRKGDRIILEA
                     NEEYWAGRPYLDKIVFRSIPDNGARYMELQSGTIDMMDGINPEDVEGIKENEKFKIIL
                     RPSLNVGYLAMNFVREPFDDKLVRQAVSHAINKEALIGAFFAGLAEPAKNPIPPSMWG
                     YNDEIEDYNYNIDQAKSLLEEAGYPDGFEFDLWYMPVPRPYMPQPKLIAQSIQYYLSE
                     INVKANLVSYDWGTYLEKVNNKDEADTYLLGWYGDNGDPDNFLYVLLDKTNSNNNYKN
                     EELHEILVEAQTTMNHQKRVELYEEAQVIIHDDIPWVPLAHSTPPIALKNEVKNFIPD
                     PNKTEKFTEVWMEK"
     gene            complement(4285..5076)
                     /locus_tag="VKN64_00160"
     CDS             complement(4285..5076)
                     /locus_tag="VKN64_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA58673.1"
                     /translation="MDLKIKGNSALITASSSGLGKAVAEAMVKEGVNVVINGRDEERL
                     NETIKELSALNKGKVVGQVGDITKKEDIVKLVEKTYNTYGSLDHLVTSAGGPPSKPFM
                     ETTDEEWYKTFDLLVMSVVRLVREAKKYLEEGEGGTVVNLTSRSVKEAMEGLVLSNSV
                     RMSVIGLMKTLSKELGPKIRVNAILQGLHETDRLKNLLEKQVEDGKFKTYQEALAAKS
                     NDIPLQRIGEPIELGEVVTFLSSKRSSYLNGVSIVLDGGGSNSNL"
     gene            complement(5115..6017)
                     /locus_tag="VKN64_00165"
     CDS             complement(5115..6017)
                     /locus_tag="VKN64_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HMA58674.1"
                     /translation="MKLNTLKIFVKLIDTGSYSKAAEKMGLTQPAISMQIKTLEERFE
                     TDLVYKEKGNINLTPAGKAVYKNAKEIIDRWDKLVIDVEDKKDKLYKNIIIGSSTIPS
                     EYILPDLLADFSKNEPEIKSCIEVGDSADMIELLEAGEVDVILVGFKPASSRYETIEI
                     VEDSLRLITPVTHRLVEKESVKVKDLIEENLLIREVGSGTRKAMLKGFKNAGIQFNDL
                     NIKCRLGSTEAIIAAVQAGLGISLISKFASAKAKKHGRIEEIEVEDITFSRKFYLAFN
                     KNRKDDELINRFIDSCQRLFIEET"
     gene            complement(6028..6501)
                     /locus_tag="VKN64_00170"
     CDS             complement(6028..6501)
                     /locus_tag="VKN64_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HMA58675.1"
                     /translation="MLEIKIPGYKTLNIQHLVLDFNGTIAKEGKLIEGVKEMLDKISG
                     IIEVHILTADTFGTVRDEFSGNEINIEIIDENQRSIDYKKEFIKSLGKESVIAIGNGN
                     NDAKMLKTAELGIAIIGGEGAAISSLWNAEVAVNSIMDALNLITNKKGLIATLRR"
     gene            complement(6515..6691)
                     /locus_tag="VKN64_00175"
     CDS             complement(6515..6691)
                     /locus_tag="VKN64_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58676.1"
                     /translation="MAKSLKQIIKNFYNNMVNTTDTGQDLEEKIEKENRSFIKEIVDF
                     FIEKFNFLKPKNKE"
     gene            6842..6994
                     /locus_tag="VKN64_00180"
     CDS             6842..6994
                     /locus_tag="VKN64_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58677.1"
                     /translation="MSTSGKGFKEGSYKCKNCGKVIKLDSFSNSLPKCPNCKSTKYSQ
                     IGSERA"
     gene            complement(7044..8813)
                     /locus_tag="VKN64_00185"
     CDS             complement(7044..8813)
                     /locus_tag="VKN64_00185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HMA58678.1"
                     /translation="MEMKWSLDSLYPGFDAIKFKEDRSKFEDKIEELEEWNFAEGNKL
                     ENIEDFILTLKDYYKLSMRLKAFSHLTVSVDAKNEEALKMIEKIEKEMVRTTKPMVNF
                     QLYLSEIDNRESLYQESEILAEHKFFIEELVDKSKYLLPEDEEELIAKMKQTGSSAWT
                     KLQEKLTSTLKVPIEIEGEEKELPLPVVRNMAYKNDPEIRRKAYQAELDSYEEIEESV
                     AAALNGIKGEVLTLTERRGYDSPLAEALHDSRLKKETLDALMAAIEEKLPLFREFYKT
                     KAEILGHDKGLPFYDLFAPLGEKEMEFSYQEAKNFILANIEDFSQEMADLYKNAFENQ
                     WIDAEPREGKRGGAFCYNLHPIKESRILSNFTGSFSDVTTLAHELGHAYHGHSLEEES
                     IINSNYPMPLAETASILSETIVTEAALEKADQEQTYSILENYITSAGQVIVDIYSRFT
                     FEKNLFKEREASSLSVDELKELMVEAQKKAYGDAIDHNYLHPYMWLNKPHYYSAGNNY
                     YNFPYAFGLLFGLGVYALSQQKDGQFMEDYNELLRATGKKKIESVAEMVDIDVTSKEF
                     WLSSLEVIENNINRFKQIAEKKI"
     gene            complement(8889..9203)
                     /locus_tag="VKN64_00190"
     CDS             complement(8889..9203)
                     /locus_tag="VKN64_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HMA58679.1"
                     /translation="MFTVNTEEIEGKEITEVLGLVRGNTIRARHIGNDIVAGLRNLVG
                     GEVKEYTQMISEAREEALIRMEEEAQKLNADAVVNVRFTTSQVMGGAAEILAYGTAVK
                     LK"
     gene            complement(9190..9891)
                     /locus_tag="VKN64_00195"
     CDS             complement(9190..9891)
                     /locus_tag="VKN64_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HMA58680.1"
                     /translation="MYPCIEIKDENPFSAGIKKIYDILYTAYGPQDWWPGDSQLEIII
                     GAILTQAVRWKNVEKAIDNLKANGLLDINRLDQIEQDKLAQLIKPAGYYNMKAKKLKS
                     FIYFLKEDYNSNLDNLAKGKPDKKREELLEIYGIGPETADSILLYAFNKPVFVIDTYT
                     KRILSRIGYIKANISYQKLQAKIENNLKTDKELYNEYHALLVKHAKEHCLKSSPLCKG
                     CPINNFDKEERYVYG"
     gene            complement(9896..11287)
                     /locus_tag="VKN64_00200"
     CDS             complement(9896..11287)
                     /locus_tag="VKN64_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA58681.1"
                     /translation="MSKENYDVIVVGTGPTGIFTVMELLKKNGNLDILMLEKGKDISE
                     RECPMKQNPGMGCINCDSCSVVSGWGGAGAYSDGKLSLSPKIGGHLEEYIGLDKLREL
                     ISYADDIYLNFGAPDRVFGVDGSKVQDIKEKAIKSQLKFIPAKLRHLGTGYTKTVLTN
                     MKNYIESEGVDIKFRTNVKDFIIEDSTIIGVKTADGTEYYGEYVITAPGRENASWLSS
                     LAPELGIETSINPVDIGVRVETSAVIAKQLTQDLYESKLIYQTPTFDDKVRTFCMSPE
                     GEVVNENNQGLITVNGHSHADIKTENTNFALLVSKTFTEPFHEPITYGKNIAKLANLL
                     GGGVLIQRLGDLLDGRRSTPSRIDRGIIEPTLKEATPGDLSLVFPYRHLKGIIEMLEA
                     LDNLAPGLYSRDTLLYGVEVKFYSNRISVNDNLETKINNLYTAGDGAGITRGLMQASV
                     SGIVIARDILNKE"
     gene            complement(11306..12841)
                     /gene="guaA"
                     /locus_tag="VKN64_00205"
     CDS             complement(11306..12841)
                     /gene="guaA"
                     /locus_tag="VKN64_00205"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMA58682.1"
                     /translation="MVGNKVLILDFGGQYSQLIARRVREFNIYSLIKSYKISSKEIKE
                     IEPVGIIFSGGPNSVTSPNAPQIDPEILNLDIPILGICYGMQLMASLMPGGIVKKAKQ
                     GEYGKAKLKIDKEIGILKDIEDNTQVWMSHGDQVKNMPDGFKNIAHTEVTDVAVMADV
                     NKKLYGVQFHPEVLHTLKGKEILHNFLFRECKAKADWTMADYIDEQVSKIRDEVNDQQ
                     VICGLSGGVDSSVASALVYKAIKEQLTCIFVDHGLLRKGEAAEVKRTFSEEFNIPLVY
                     VDAKERFLNKLEGVSDPEAKRKIIGEEFIRVFEEEASKVENASYLVQGTIYSDVIESG
                     NEHAATIKSHHNVGGLPEEMNLDLIEPLRMLFKDEVRKIGEVLGLPAEIVWRQPFPGP
                     GLAIRIIGEIDDKKLAILKNADAILREEIKEAGLSREIWQSFAVLPDLRSVGVMGDDR
                     TYGYPIILRAVKSDDAMTADWAHLPYDLLEVISNRIVNEVKEVNRVVYDITSKPPATI
                     EWE"
     gene            complement(12970..14631)
                     /gene="groL"
                     /locus_tag="VKN64_00210"
     CDS             complement(12970..14631)
                     /gene="groL"
                     /locus_tag="VKN64_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553763.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMA58683.1"
                     /translation="MAKDIKFSEEARRKLKSGVEKLANSVKVTLGPKGRNVLLEQSFG
                     APTITNDGVSIAKEIEVKNRYENMGVQAVKEVATKTNDVAGDGTTTATVLAEAIFNEG
                     LKNVTAGANPMLLKRGIAKAVEKLTEEIANTATPVEGREEVSQIASISAGNDDEIGDL
                     IAEAMEKVGQDGVISVEESKSMGTSLEVVEGMQFDRGYLSPYMVTDTEKMEASLEDPY
                     ILITDKKISNIQDILPLLEKVAQSGKSLLIVAEEVEGEALATLVVNKIRGTFDCVAVK
                     APGFGDRRKQMLEDLAILTGGRVITEDLGLKLENAEISDLGQAHKITITKDDTTIVEG
                     EGNKEDLKERISQIRRQIESSDSDFDKEKLQERLAKLAGGVAVIRVGAATETELKEKQ
                     HRIEDALSATRAAVEEGLVPGGGVTYLNAMKVLDDIKLEGDEGTGVDIVKRSLTAPMR
                     LIANNAGYEGSVVVEKIKELEPGMGFDAMKGEYVNMIKAGIVDPAKVTRSAIQNAASA
                     AAMLLTTEALVADTSDDDDDENGGGAAPGGMPGGMGGMGGMPGMM"
     gene            complement(14676..14957)
                     /locus_tag="VKN64_00215"
     CDS             complement(14676..14957)
                     /locus_tag="VKN64_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMA58684.1"
                     /translation="MSVKPLGDRVAVKLVEREEKTEGGIVIPDTAKKEKPQTGEIYAV
                     GRDCYTEDETPEVEKGDMVVFDKFAGTEVTVDGVEFKIVNIDDIVAVIG"
     gene            complement(15169..>15924)
                     /locus_tag="VKN64_00220"
     CDS             complement(15169..>15924)
                     /locus_tag="VKN64_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HMA58685.1"
                     /translation="RVFMPGYTSLTAADLKFDHKEVYQKLSKMVDEINTFQKTPVTIE
                     PPMIKNLKSKVKGIIKRSPADKANVKYLDIIKKVNNQETITRVETFNTIKKLKNPVLE
                     IKREDKVIKTTLKKEKEKRSGLIMDYDLSLNKITEINNLIKNNEDKKILFLTSELGYH
                     LIKYIIKNYLDYADKNNIFIEALKNTYLKGSIISAGLLTNLDIENHLINKTDLKPDLL
                     ILPAVMYDIFNNDLRGNSYKSIENKQMFEVEIL"
BASE COUNT         4622 a         3190 c         2357 g         5755 t
ORIGIN      
        1 ttagcttctt tttcagcgga agaaagggct ttataatata cttttccccc ttcaccgcgg
       61 gctgccttta aacgtttggg caccctattt ttattatttt caataacatt atcagccaca
      121 tctaaaatct ttttatgaga ccggtagttt tgttccagtt tgatcacttt agcatccgga
      181 taatcttctt caaagtttaa aatatttcta atgtcggctc ccctgaaagc atatatactc
      241 tggtccgggt cgccgaccac aaagatattt ctatacttgc tggctaaaag ttgagcaaat
      301 ttatactggg caaagttaac atcctggtat tcgtcaatta atatatatct atacctctcc
      361 tgatagcggt ttaaaacctc tgggtaatct aaaaatagtt caacggtttt cataattaaa
      421 tcatcaaagt caagcgcatt attatttttg agtctctttt catattcttc atagacggca
      481 gcaactatct tttcaaagta accgatggca tctttttgaa aagcagctgc ttcaattaat
      541 tcatttttag cattacttat tctatttaat actgcagaag gacgaaactt tttaacatca
      601 tagttaagat cttttaagat atttttgacc atcttttttt ggtcataaga atcatagatc
      661 gtaaagtatc tattaaattc caacctgttg atttcctgtc ttaaaatctg tacacaaaaa
      721 gaatgaaaag tacccaaaaa tatatcgtct gcatctcggc ctgtcatctt ttggaccctt
      781 tccttcatct catcggcggc tttattggta aaggtaaggc ccaaaatttg atagggttta
      841 acctcatatt tttctattaa ataggcaatg cgattggtta ataccctggt tttacctgaa
      901 ccggctcccg ctatgataag gccggggcct ttatgtaatt taacagcctt ttcctgctgt
      961 tcatttaatt tagcaataat ttcttttgct ttatccatta atgattcact cctgattgaa
     1021 ttattatatg tcttttttaa agtcttctag catatttttg agattcagat cgttttttga
     1081 gtataataaa tgggatatac agctgcagtc gctggaacca aaaccttcga ccattagttt
     1141 gaactgatct ttatggtaca aataattaaa gagcttgcac tcataatctt tggaaccatt
     1201 taaagtgaaa taatcatcta tcttggcttt atcaagcaga taatctatat gccagtgaaa
     1261 tttctttttt gttctcagat gtctttcgat tctgctctct aaattttttt gggcagaacc
     1321 aatatagtaa taataacctt ttttaaagtt attttcacct aaagctccta ctttgattga
     1381 ttggttttta gttagcttga tttttacaat atatataccg ctgttttcag cttccattta
     1441 ttgtccccta actattttac ttcgatgatt tgaatgcgca atccttcctt taataaacaa
     1501 tctttactaa agatttgatt aatgttataa ttaaaaaaga ctaaccaata atttgatatt
     1561 ttttgtactt ttatctggct atagcgcagc ttggtagcgc gcttgcttcg ggagcaagag
     1621 gtccccggtt caaatccggg tagccagacc atttctatat gtatgacact aaataattat
     1681 aacataaatg aggcgggttt gcattttatt gaagttcaga cttaatgctt aaataacaag
     1741 ttacaaattt tataatacat aaaaaaagag tgatgactat cttatatagt tatcactctc
     1801 tttttaattt tattctttta attcttttaa aaattcaact actctttcgg catgaccatc
     1861 aaccttaact ttgggatata ccttcattat tttttgattt ttagaaatta taaaggttga
     1921 cctcttatat atccccacta aatttaccac atcgaacttc ttggccagtt ttttatctgt
     1981 atctgctaat aatgggtaag tcaagtcata ttcttctgcg aaattcttat gtgactcaac
     2041 actgtctttg cttaccccta caaccttagt atttaacccc tccagggttt ttagctgcct
     2101 ttgaaaggca accgattcct tttttcagcc cggtgtattg tctttaatat agaaaaacaa
     2161 aactaatctc tggtctttaa aatcatcccg acagtgttct ttaccatctt gatcaggcag
     2221 acaaaaatca ggtaaactat ctcctacttt taacattaaa ttcttacctc ctttttgaaa
     2281 taaggtctac attaatattt ttaattaata ttatccctta attatttgaa atcttaattt
     2341 tgagagtata attcatttta tatttatttt aaaaaaatat aattaaacct actatttctt
     2401 ctttctggtt acttttatag attttatata tctataattg aatatttata attaaatacc
     2461 tccattttta attcaattaa atgcaactaa attgaaatat attcttgcta agttaataat
     2521 tataatataa gatgtatata tatttgaaat taaataatac aagaagggag tatggtaaat
     2581 gtcaagattt aatattacaa tcactatttt gctggtatta gtattaacac ttagttttac
     2641 cgtctcagct gaagaaggta catatggagg tactcttgtc tatggacgcg gaaatgattc
     2701 tgtaagcctt gacccaatta atactaccga tggcgaatct tttaaggtaa cacaacaaat
     2761 ttatgacacc ctacttatgt ataagcctgg tactacagaa ccagtgcctt gtttagccaa
     2821 agagtgggaa gtaacagaag atggaaaaaa atggactttt tatttaagag aagatgttaa
     2881 ttttcatgat ggtaatccct tcaatgcaga agcagttaaa ttcaactttg atcgttggcg
     2941 ttttgaggac aatccttatc atgtaggagg aacatttgct tcttaccctt atgtttttgg
     3001 tggttttccc gggataatca aagaagttaa tgtagtaagt gattatgtag tagaattcgt
     3061 tttagaaact ccacaagctc cattcttagc taatatggca atggtttctt ttgcgatagc
     3121 aagtcctgaa gctgtaaaag aatggggaga aaattacttt aaacacccgg taggtactgg
     3181 accatttgaa tttgttgaat ggagaaaagg cgatagaatc atattagaag caaatgaaga
     3241 atactgggca ggtcgcccct atttagataa aatagtattc cgctcaattc ctgataatgg
     3301 cgcacgttat atggaattac aatcaggtac tatagatatg atggatggta ttaatccgga
     3361 agatgtagaa ggtattaaag aaaatgaaaa attcaagatt atcttacgac ccagtctcaa
     3421 tgtaggttat ctagccatga attttgtaag agaacctttt gatgacaagt tagtaagaca
     3481 agctgtaagt catgctatta ataaagaagc attgataggt gccttttttg caggtttagc
     3541 agaaccagcc aaaaatccaa taccaccttc catgtgggga tataatgatg aaatagaaga
     3601 ttacaactat aatattgatc aagctaaaag tctcttagag gaagcaggat acccggatgg
     3661 atttgaattt gatttatggt atatgcctgt tcctcgccct tacatgcctc aacctaaatt
     3721 gattgctcaa tcaatccaat attatttaag tgaaattaat gttaaagcta acttggtttc
     3781 ttatgattgg ggaacctatt tagaaaaagt taataataaa gatgaagccg acacttatct
     3841 tttaggctgg tatggagata atggagatcc tgataacttc ttatacgtat tattagataa
     3901 aacaaactct aataacaatt acaaaaacga agaattacat gaaatactgg tagaagcaca
     3961 aactaccatg aatcatcaaa aaagggtaga attatatgaa gaagcacaag ttattatcca
     4021 tgatgatatt ccatgggtac ccttagccca ttctacacca ccaatcgctt taaaaaatga
     4081 agttaaaaat ttcattccag atcctaataa aactgaaaaa tttacagaag tatggatgga
     4141 aaaataatta aattatattg atatatagat tctataaata taaaagcctc ccctttatta
     4201 aagtaagtag tttaagctat tcttcttact taaaggggag ttttatttta tcaattatat
     4261 atttaatttg gcttaaaaat aatattataa gttggaatta ctacctccac catctaagac
     4321 aattgaaaca ccatttaaat aactagatct ttttgaagaa agaaatgtta caacctctcc
     4381 caattcaata ggttcaccga ttctttgtag gggaatgtcg ttacttttag cagccagggc
     4441 ctcttgatag gttttaaatt taccgtcttc tacctgtttt tctaataaat tttttaatct
     4501 atctgtttcg tgcagaccct gcaggatagc gttaactcta attttgggtc ctaattcctt
     4561 agaaagtgtt ttcatgaggc cgatcacact catgcgaaca gaattcgaaa ggaccaatcc
     4621 ttccatagcc tctttaacac tgcgggaggt aaggttaacc acagtacctc cttcaccttc
     4681 ttctaaatat ttttttgctt ctcttaccaa tctcactaca ctcatgacta acaggtcaaa
     4741 agttttatac cattcttcat cggttgtttc cataaatggt ttgctgggcg gtccaccagc
     4801 cgaagttacc aaatgatcta aacttccata ggtattataa gttttctcaa ccaacttgac
     4861 aatatcctct tttttggtga tgtctcctac ttggccgact acttttcctt tatttagggc
     4921 acttaattct ttaatagttt catttaatct ttcttcatct ctaccgttaa taacaacatt
     4981 gactccttct ttgaccatag cttctgctac tgcttttcct aatccactac ttgaagccgt
     5041 tatcaaagca ctatttccct taatttttaa gtccatatta aatactccct tcttttatat
     5101 atttgtttga tttattaagt ttcctcaata aataatctct ggcaggaatc gataaaacga
     5161 tttattaatt catcatcttt cctgttttta ttgaaagcaa gataaaattt cctgctgaag
     5221 gtaatatcct cgacttcaat ctcctcgata cggccgtgtt tcttggcttt agcagaggca
     5281 aacttagaaa ttaatgatat acctaaacca gcctgaaccg cagcaattat ggcttcagta
     5341 cttcctaatc tacattttat atttagatca ttaaattgaa tgcctgcatt tttaaaacct
     5401 ttcaacatgg ctttgcgtgt accagaacca acctctctaa ttaagaggtt ctcttctatc
     5461 aaatctttta cctttacgct ttctttttct actaatctat gagtgactgg ggtaattaat
     5521 cttaagctat cttctacgat ttcaatagtt tcataacgac tgctggccgg tttaaagccc
     5581 actaaaatta catcaacttc tcctgcctct aaaagttcaa tcatatcagc actatcgcct
     5641 acttcaatgc aagatttaat ttcaggttca tttttggaaa aatcggctaa aaggtcgggt
     5701 aaaatatatt ctgatggtat agtagatgaa cctataatga tattcttata tagtttgtct
     5761 tttttatcct ccacatcgat tacaagttta tcccaacgat ctattatctc ctttgcattc
     5821 ttatagaccg ctttcccggc cggggtaagg ttaatattac ccttttcttt atatacaaga
     5881 tcagtttcaa acctctcttc taaggtttta atctgcatac ttatggccgg ttgggttaaa
     5941 cccatttttt cggccgcttt tgaataacta ccggtatcta tcaactttac aaatattttt
     6001 aaagtattta gtttcataaa atttcaccta ccttcttaaa gtagcgatta atcctttttt
     6061 attagttatt aagtttaagg catccataat actatttaca gctacttccg cattccagag
     6121 actgctaata gctgctcctt ctcccccaat tatagcaata cctaattcag ccgtttttag
     6181 catttttgca tcattattgc cattgccaat tgctatcact gattctttac ctaaactttt
     6241 tataaattct tttttgtaat caatgctgcg ttgattctca tcaataattt caatattgat
     6301 ttcgttacca gagaattcat ctctgacagt gccgaaggta tcagccgtta aaatatggac
     6361 ttcaataatt ccacttattt tatccagcat ttctttaacg ccctcgatca gcttcccctc
     6421 tttagcaatt gttccgttaa agtctaaaac taaatgctga atattgagtg ttttgtagcc
     6481 gggtatcttg atttccaaca tttaaatcac ctgcttattc tttatttttt ggcttcagga
     6541 aattaaattt ctcaataaaa aaatcaacga tttctttaat gaaagatcga ttctctttct
     6601 ctattttctc ctctaaatct tgcccagtat cagttgtatt aaccatgtta ttataaaaat
     6661 ttttgataat ttgttttaaa ctttttgcca cttagaccac tcctatacgc ggtttctatt
     6721 tattattata gtacacaaag tacagcaaaa tcaaaaattg tcaaaatttt gtaaatttac
     6781 ttttaatatc ggtatcttta ttgtataatt aaaatacatt aaatcttagg gaggtgtcat
     6841 tatgtctacc tctggtaaag gatttaaaga gggcagctat aaatgtaaaa actgtggtaa
     6901 agtaattaaa ttagactctt tttctaattc acttccgaaa tgcccgaact gtaagagcac
     6961 aaagtactct caaattggta gtgagagagc ataattaaat aaatattggt aaagccgggt
     7021 ttaaaaaccc ggctttttta atattatatc tttttttctg ctatttgttt aaatctatta
     7081 atattatttt ctataacttc taaagaactc agccagaatt cttttgatgt tacatcgata
     7141 tcaaccattt cagctacaga ttcgattttc ttcttgcccg tagccctcaa tagttcgtta
     7201 taatcttcca taaattgacc gtctttttgt tggcttaaag cataaactcc taggccaaat
     7261 aatagaccaa aggcataagg gaaattatag taattattac cggccgaata gtaatgaggt
     7321 ttgttaagcc acatgtaagg atgtagataa ttatggtcaa tagcatctcc ataggccttc
     7381 ttttgggctt caaccattaa ctctttcagt tcatcgacac ttaaagaact agcttctctt
     7441 tctttgaaaa gatttttttc aaaggtaaaa cggctgtata tatctacaat tacctgaccg
     7501 gcggaagtaa tataattttc taatatggaa taagtctgct cttgatctgc cttttctaaa
     7561 gcagcttcag ttacgatagt ttcacttaag atagaagcgg tttcagctaa aggcattgga
     7621 taatttgaat ttataatact ttcctcttcc aaactgtgtc cgtgataagc atgacctaat
     7681 tcatgggcta aagtagttac atcactaaaa gatccggtaa aattacttaa gatccggctc
     7741 tcttttatgg gatggagatt ataacagaag gcgccccctc ttttaccttc tctcggttca
     7801 gcatcaatcc attgattttc aaatgcgttt ttatatagat cagccatctc ctgactgaaa
     7861 tcttcaatat ttgcaaggat aaagtttttt gcctcttggt aactaaattc catttctttt
     7921 tctcccagtg gtgcaaagag atcataaaat gggagacctt tgtcatgtcc taatatttca
     7981 gctttagttt tatagaactc tctaaataag ggtaacttct cttctatagc agccattaga
     8041 gcatctaagg tttctttttt taagcgcgaa tcgtggagcg cttcagctaa aggagaatca
     8101 taacccctgc gctcagttaa agttaaaacc tctcctttta ttccgtttaa ggcagctgcc
     8161 accgattctt cgatctcttc ataagaatcc agttcagcct ggtaggcctt ccttcttatc
     8221 tcgggatcat ttttatatgc catatttctg accaccggta agggaagttc cttttcttct
     8281 ccttcaattt caatcggtac tttcaaagta gaagttaact tctcctgcag tttagtccag
     8341 gcagaagaac cggtctgctt cattttcgct attaattctt cttcatcttc gggtagtaga
     8401 tatttacttt tatccactaa ttcttcaata aaaaatttgt gttcagctaa aatttcggat
     8461 tcctgataaa gggattccct attatctatc tcactcaagt agagttgaaa atttaccatt
     8521 ggttttgtcg tcctgaccat ttctttttct atcttttcta tcatctttaa ggcttcttca
     8581 tttttagcat ctacacttac ggttagatga ctaaaagctt ttaatctcat tgataactta
     8641 taataatctt ttagggtaag aataaagtct tcaatattct ctaatttatt accctcggca
     8701 aaattccatt cctctaattc ttcaatttta tcctcgaatt tacttctatc ctctttgaat
     8761 tttattgcat caaagccggg atagagtgag tccaaactcc atttcatctc catagataac
     8821 actcctcata tttttataaa ttctcatctt aaatagatta aaccgggttt aaacccggtc
     8881 taattacgtt attttaattt gacggctgtt ccatatgcta aaatttcggc tgctcccccc
     8941 atgacctggg aagtagtgaa gcgtacatta actaccgcat ctgcattgag tttttgggct
     9001 tcttcttcca tcctaataag ggcttcttca cgcgcctcac tgatcatctg ggtatattct
     9061 tttacttctc ctcctacgag atttcttaaa cccgcaacta tatcattacc aatatggcgg
     9121 gccctgatag tattacccct taccaaacca agaacttcag taatttcttt accttcaatt
     9181 tcctccgtat taaccgtaaa catatctctc ctccttatca aaattattaa ttggacatcc
     9241 tttacataaa ggggagcttt ttaaacagtg ctctttagca tgtttaacta ataaggcgtg
     9301 gtattcatta tatagttctt tatcagtttt taaattattt tctatctttg cttgcagctt
     9361 ctgataagaa atgttggctt taatatatcc tatcctggat aatattcttt ttgtataggt
     9421 atcaattaca aatactggct tattaaaagc atatagaagt atcgaatccg ctgtttcagg
     9481 ccctatgcca tatatttcaa gcagctcttc acgttttttg tcaggcttcc cttttgctaa
     9541 attatctaaa tttgaattat aatcttcttt gagaaaataa atgaaagatt ttagcttctt
     9601 tgctttcatg ttataatatc ccgccggttt tatcaattga gctaacttat cttgttcaat
     9661 ttgatctaac ctattgatat ctaataaacc attcgctttt aaattatcta tggctttttc
     9721 aacatttttc catctgacag cctgggttag aatagctcca ataatgatct cgagctggct
     9781 gtctcccggc caccaatcct ggggaccgta ggctgtataa agaatatcat atattttttt
     9841 tatacctgct gagaaaggat tctcatcttt aatttcaata cagggataca tttaatcatt
     9901 ccttgttgag aatatctctc gctattacta taccggaaac tgaggcctgc attaaacctc
     9961 tggtgatacc ggctccatct ccggctgtat ataaattatt aatcttagtt tctaggttgt
    10021 cattgacaga aatacgattt gaataaaact taacctctac cccatataat aaggtgtctc
    10081 tagaatataa accgggtgct aaattgtcta aggcttccag catttcaatg atgcctttta
    10141 aatgccggta gggaaatact agacttaaat caccgggagt agcctctttt aaggtcggtt
    10201 caattatgcc ccgatcaatc ctggacggtg ttgaccggcg accatctaaa agatcgccca
    10261 atctttgaat caagactccg ccccctaaaa gattggctag cttagctatg tttttaccat
    10321 atgtgattgg ctcatggaac ggttcggtaa atgtcttact aactaagagg gcaaagttgg
    10381 tattttcagt tttaatatca gcatggctgt gaccatttac agttattaaa ccctgattat
    10441 tctcatttac tacttccccc tctggtgaca tacaaaaggt ccttacctta tcatcaaagg
    10501 tcggggtctg atagattaac tttgattcat aaagatcctg ggtcagctgt ttggcaatca
    10561 cagccgaagt ttccactcta accccaatat caaccggatt aatgctggtc tcgattccca
    10621 gttccggtgc tagggaactt aaccaggagg cattttctcg cccgggggca gtgattacat
    10681 actccccgta atattctgtt ccatctgcgg ttttaactcc gatgattgta ctatcttcaa
    10741 tgataaaatc ttttacattg gttctaaact taatatctac tccttcactt tcgatatagt
    10801 ttttcatatt agttaaaact gttttggtat agcctgtacc aaggtgtctt agcttggcgg
    10861 gaataaattt aagctgtgat ttgatcgctt tttcttttat atcttgtacc ttagaaccat
    10921 caaccccaaa tactctatcc ggtgctccaa agtttagata tatatcatca gcataactga
    10981 tcaattctct taatttatct aatcctatgt attcttctaa atgtccgcct atcttagggg
    11041 aaagcgataa tttgccatca ctatatgccc cggcaccacc ccagcctgaa acgactgaac
    11101 agctatcaca gttaatacag cccatgccgg gattttgttt catcggacac tctcgttctg
    11161 agatatcttt tcctttttct aacattaaaa tatctaaatt gccattcttt tttaaaagtt
    11221 ccattacggt aaatattcca gttggtcctg tcccaaccac gattacatca tagttctctt
    11281 tactcattga ttttgcactc ccatcctatt cccattcaat tgtagcgggg ggttttgatg
    11341 taatatcata aaccactctg ttgacttcct taacttcatt gacaattctg ttcgaaatta
    11401 cttctaaaag atcataaggt aaatgagccc aatcggccgt catcgcatca tcacttttta
    11461 cagcccttaa gatgattgga taaccataag tacggtcatc acccattaca cctacacttc
    11521 ttaaatcagg taaaacggca aaagactgcc agatctctct cgatagaccg gcctctttga
    11581 tttcttctct taaaattgcg tccgcatttt ttaagatagc cagtttttta tcatcaatct
    11641 ctcctatgat ccggatcgct aaaccgggac cagggaaggg ttgtctccaa acaatttcgg
    11701 ctggcaaacc taaaacttca ccgatcttgc gaacttcatc tttaaataac attctaagtg
    11761 gttctattaa atctaagttc atctcttccg gtaaaccacc tacattgtga tggcttttaa
    11821 tagtagctgc gtgttcatta ccactttcaa tcacatccga atatattgtg ccctgaacta
    11881 aatagctggc attttcgacc tttgaggcct cttcctcaaa cactcttatg aactcttctc
    11941 caattatctt ccttttggct tccggatctg atactccttc taatttattt aaaaatcttt
    12001 ctttagcatc tacatatact aagggaatat tgaattcttc actaaaggtc cttttgacct
    12061 cggccgcttc tcctttccgg agtaggccgt ggtctacaaa gatacaggtt aattgttctt
    12121 taattgcctt atagactaag gcggaggcca cagaagagtc aaccccacct gataaaccac
    12181 agatcacctg ctggtcatta acttcatctc ttattttact gacctgttcg tcaatataat
    12241 cggccattgt ccaatcagct ttagctttac attctctaaa aagaaagtta tgtaatatct
    12301 ctttaccttt taaagtatgt aaaacttccg gatgaaactg aactccgtat aactttttat
    12361 ttacatcggc cattactgct acatcggtta cctcggtatg ggcaatattt ttaaagccat
    12421 ccggcatatt ttttacctgg tctccatggc tcatccatac ctgggtgtta tcttcaatat
    12481 cttttaaaat tccaatctct ttatctatct tcagttttgc cttgccatat tctccctgct
    12541 tcgccttttt aacaatacca cccggcatca aggaggccat taactgcatt ccatagcaga
    12601 tacccaaaat agggatatct aaatttaata tttcaggatc aatctgaggg gcattaggac
    12661 tggtcacact attcggtccc cctgaaaata ttattccaac tggttctatt tcttttattt
    12721 ctttacttga aatcttatat gattttatta aagaataaat attaaattct cggactcttc
    12781 ttgcaatcaa ctgactgtat tgaccaccaa aatcaaggat taatactttg ttaccgacca
    12841 tgaaattctc ctttcgcatt atattaaatc acaagttata ttataaggta aaaatgtata
    12901 aatgtaaata aaaaaagtgg tataccgcta aacggtacac cactttgaga gtactctatt
    12961 tactttctat tacatcatac cgggcattcc gcccattccg cccattccac cgggcattcc
    13021 gccgggagca gctccaccgc cgttttcatc atcatcatca tcagaagtat cggcgactag
    13081 ggcttcagtt gtgagtaaca ttgccgctgc acttgcagca ttttgaattg cagagcgtgt
    13141 tactttagct ggatctacga taccagcttt aatcatattt acatattcac ctttcatggc
    13201 gtcaaaaccc atgccaggtt ctaattcttt aatcttttca acaacaactg aaccttcata
    13261 accagcgtta ttagcgatta agcgcatagg agcagttaag gatctcttaa ctatatctac
    13321 accggtacct tcatcacctt ctaatttgat atcatcaaga accttcatag catttagata
    13381 ggtgactcca ccacctggta ctaatccttc ttctacagct gctctggtag ctgataaggc
    13441 gtcttcaatt ctgtgctgtt tttctttgag ttcggtttcg gttgcggcac cgactctaat
    13501 tacagcaaca ccaccagcta acttagcaag tctttcttgt aatttttctt tatcaaagtc
    13561 agagtcactg ctttcaatct gacgtctgat ttgactaatt ctttctttca agtcttcttt
    13621 atttccttca ccttcaacaa tggtggtatc gtctttagtt attgtaattt tatgagcctg
    13681 acctaggtca gaaatttcag cattttctaa tttaaggccg aggtcttctg ttattacacg
    13741 gccgcctgtt agaattgcta agtcttctaa catttgcttt cttctgtcgc caaagccagg
    13801 tgctttaacg gctacacaat caaaagtacc tctaatttta tttacaacta aggttgcaag
    13861 tgcttcgcct tcaacctctt ctgctactat aagtagagat ttaccggact gagcaacttt
    13921 ttctaataga ggtaagatat cttggatatt tgaaattttc ttatctgtga taaggatgta
    13981 tggatcttca agagaagctt ccatcttttc agtatcagtt accatgtaag gagataagta
    14041 acctctgtca aactgcatac cttcaactac ttctagggaa gttcccatac tcttagattc
    14101 ttctacggag attactccgt cttgtccaac cttttccatt gcttctgcaa ttagatctcc
    14161 aatttcatca tcattgccag cagaaattga agctatttga gaaacttctt ctctgccttc
    14221 aacaggagtt gcagtatttg caatttcttc ggttagtttt tcaactgcct tggcaatacc
    14281 tcttttaagc agcattggat tggcgccagc agtaacgttt ttgagacctt cattgaaaat
    14341 agcttcagct aatacggtag cggtagttgt accatcacct gctacatcat tagtcttagt
    14401 agctacttct ttaactgcct gtacacccat gttttcataa cgatttttaa cttctatttc
    14461 tttagcaata ctcacaccat cattagtaat ggtaggagca ccaaaactct gttctaataa
    14521 aacatttctt cctttaggtc ctaatgtgac cttaactgaa tttgctaatt tttctacacc
    14581 actttttaac ttacgacgtg cctcttcgct aaacttaata tctttagcca ttatttataa
    14641 accctccctt agttagttat tactttcgtt taaaattaac caattactgc aacaatgtcg
    14701 tcaatattga ctattttgaa ttcaactcca tcaactgtta cttcagtacc agcaaattta
    14761 tcgaatacta ccatatctcc tttttcaact tcaggtgttt catcttcggt atagcagtca
    14821 cgacctactg catagatttc accagtttgt ggcttctcct ttttagctgt atctggtatt
    14881 acaataccac cttcagtttt ttcttctctt tcgactaatt ttactgctac tctgtctcct
    14941 aatggcttaa cactcattta atgtaccccc tttaatttta tatataattt atttttttac
    15001 ctgttattag cactcatttg aggtgagtgc taataattac tgtattaata atatctaagt
    15061 attaatttaa aatcaagcat atttatgagt tctgagagtc tattaaagtt aattaatacc
    15121 tatatactcc aaaatattct ttttttctcc cataatatat actattgatt aaagtatttc
    15181 cacttcaaac atctgtttat tctctatact tttataactg tttcctctca gatcattatt
    15241 gaaaatatca tacattacag ccggtaaaat taatagatct ggtttaagat cagttttatt
    15301 tataagatgg ttttctatat ccaaatttgt gagaagacca gctgaaatta tcgaaccttt
    15361 aagataagta tttttcaacg cttcaataaa gatattattc ttatcagcat aatccaaata
    15421 gttttttatt atatatttta ttagatgata acccagttca gaggttaaaa acaatatttt
    15481 tttatcttca ttgtttttta tcaagttatt aatctcagta atcttattta aagaaaggtc
    15541 gtagtccatt ataagaccgg accttttctc tttttccttt tttaacgtgg tcttaataac
    15601 cttatcttct ctttttattt ctaacacagg attttttaat ttttttattg tattaaatgt
    15661 ttccacccgg gtaattgtct cttggttgtt aacttttttt attatatcca ggtacttgac
    15721 atttgcctta tcagccgggc ttctttttat tatacccttt actttacttt ttaaattctt
    15781 gatcatcggc ggctcaatag taaccggtgt tttttggaac gtattaatct catctaccat
    15841 tttagataat ttttgataga cttctttgtg atcaaacttc aaatcagccg ctgttaagct
    15901 cgtataaccc ggcatgaaaa ctct
//
LOCUS       DATGCJ010000007         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_313339, whole genome shotgun sequence.
ACCESSION   DATGCJ010000007 DATGCJ010000000
VERSION     DATGCJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313339"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VKN64_00225"
     CDS             <1..372
                     /locus_tag="VKN64_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HMA58686.1"
                     /translation="KRYLECNFQTITDHKDEIIGAVMVLIDVTRLRKLEEMRKDFVAN
                     VSHELKTPLTSIKGYADTIAENEIKDFDTIKRFVSIISKEATRLNLLINDLLDLSKLE
                     ADYFELKPEELDNVLDKPLRI"
     gene            442..783
                     /locus_tag="VKN64_00230"
     CDS             442..783
                     /locus_tag="VKN64_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA58687.1"
                     /translation="MNKSQIENLLINLIDNAIKYNKESGKIFIRAYQENGKVYLEVED
                     TGFGIPKEDQDRIFERFYRVDKARSKEVGGTGIGLSIVKHIVKGHNSEINVKSVEGQG
                     TNFKFYLHVAR"
     gene            906..1667
                     /gene="pstB"
                     /locus_tag="VKN64_00235"
     CDS             906..1667
                     /gene="pstB"
                     /locus_tag="VKN64_00235"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMA58688.1"
                     /translation="MSSNIKIKIKNLNFFYDDFQALTDINMKIKENKITALIGPSGCG
                     KSTFLRVINRMNDLIDGTTVQGEVILDGQNLYSSDVDVVQLRKKVGMVFQHPNPFPKS
                     IYDNVAYGPKIHGMKDKNKLEQLVEDSLKGAALWEEVKDRLDQPALDLSGGQQQRLCI
                     ARALAIKPEVLLMDEPASALDPVATAKIEELMAELEKDYTIVIVTHSMQQAARVSDET
                     GFFLMGELIEYGDTGKIFENPDDQRTEDYITGRFG"
     gene            1687..2340
                     /gene="phoU"
                     /locus_tag="VKN64_00240"
     CDS             1687..2340
                     /gene="phoU"
                     /locus_tag="VKN64_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMA58689.1"
                     /translation="MLRKGLDENIEKLKNEMLLMGSQVEEAIEKSIQALKEQDIELAE
                     EVMEEDDQIDEYELELEEMCTRLIATQQPVAKDLRTIIVISKLATDLERIGDHASNIA
                     KMVIKIGDEPLLKPLIDIPRMTDIVTRRLKKSLEAFVNLDVELAHEIAKEDEEVDVLD
                     EQILRELLTFMIEDPKTIRQAMSLIFISRFLERIGDHATNICERVIYMATGDWDVSY"
     gene            2375..3073
                     /locus_tag="VKN64_00245"
     CDS             2375..3073
                     /locus_tag="VKN64_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA58690.1"
                     /translation="MSNKILIVDDEKNIRDLVKYNLEEIGYDILEAEDGETAINKAKK
                     DVDLVILDLMLPKIDGLEVCKRIRNNEKLKDTAIIMLTAKDEELDRILGLEIGADDYM
                     TKPFSIRELRSRIKAVLRRTKSDFNKVKSDNDKSKFVTGDLELNIQGYEVKKKGRTLE
                     MTPKEFELLRFMFTNQNKVLTREILLEKIWGYEYTGDTRTVDVHIRRLRKKIGREYIK
                     TIRGLGYKLVYSKK"
     gene            3222..>3601
                     /locus_tag="VKN64_00250"
     CDS             3222..>3601
                     /locus_tag="VKN64_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMA58691.1"
                     /translation="MRKSLLILLSGLLVMALFSGVVGAQNNLTIQGSSTVLPIAQRIA
                     EVYMQNNEDVNISVRGGGSGNGIAALIDGTTDIADASRFIKQSEVENAVNNGYYPVPH
                     RVAMDGIAVVINPANPVESLTLDE"
BASE COUNT         1372 a          445 c          710 g         1074 t
ORIGIN      
        1 aaaaggtatt tagaatgtaa ttttcaaact attaccgacc ataaagacga aataatcggg
       61 gcggtaatgg ttctaataga tgtaactaga ctaagaaaac tagaagagat gagaaaagat
      121 tttgttgcta atgtatctca tgaattaaaa acccctttaa cctctattaa gggttatgca
      181 gatacaattg cagaaaatga aatcaaagat tttgatacta ttaaaagatt tgtaagtatt
      241 attagtaaag aagcaacccg actcaattta ttaataaatg acttattgga tttatctaaa
      301 ttagaagcag attattttga acttaaacca gaagagctgg ataatgtatt agataaaccg
      361 ctaagaattt aggaaaatga agctgataaa aaagatattg aaattataaa acaatttgag
      421 gaagatctac cgttagttaa gatgaataaa tcacagattg aaaacctcct tatcaattta
      481 atcgacaatg ctattaaata taacaaagaa agcggtaaga tattcattag ggcctaccag
      541 gagaatggta aagtatatct cgaagttgaa gatactggtt ttgggattcc caaagaagat
      601 caggatagga tattcgaaag gttttaccgg gtggataaag cccgttcaaa agaagttggc
      661 ggtactggta taggcctttc aatagtaaag cacattgtta aaggtcataa tagtgaaata
      721 aatgtaaaaa gcgttgaagg ccagggtact aacttcaaat tctatctaca tgtagctaga
      781 taaatttttt gttaacaaaa atgtaacaat aatttaacaa aaaccctgtt tataaactgt
      841 ttagcatgtg ttaaattaat aaatgtggat ttataatatt tacagaagaa aggagttcaa
      901 ccttaatgtc ctccaatata aaaattaaga ttaaaaattt aaatttcttt tatgacgatt
      961 ttcaagcttt aacagatatc aatatgaaaa tcaaagaaaa caaaataact gcattaattg
     1021 gaccatcggg ttgtggtaaa tcaaccttct tacgggtgat aaatcgcatg aacgacttga
     1081 tcgatggtac taccgttcag ggagaagtta tactagatgg tcaaaattta tatagttcag
     1141 atgttgatgt agttcaatta aggaaaaaag ttggtatggt attccaacac cccaacccct
     1201 ttcctaaatc tatatatgat aatgtagcat atggaccaaa aattcatgga atgaaagata
     1261 aaaataaact ggaacaatta gtagaagata gtttaaaggg ggcagcctta tgggaggaag
     1321 tcaaagacag gttagaccag ccggcccttg atctatctgg tggccaacag cagaggttat
     1381 gtattgcccg tgctctagct attaaacctg aagtactact tatggatgaa cctgcctcag
     1441 ctttagatcc tgttgcaact gctaagattg aagaattaat ggcagaatta gaaaaagact
     1501 atacaattgt gattgtaact catagtatgc agcaggcggc aagagtttct gatgagaccg
     1561 gctttttctt aatgggcgaa ttgattgaat atggtgatac aggtaagatt tttgagaatc
     1621 ctgatgatca aagaactgaa gattatatta caggccgatt cggttaataa ggaaaggtga
     1681 gataaaatgt tacgcaaagg acttgacgaa aacatagaaa agttaaaaaa tgagatgctt
     1741 ctgatgggta gtcaggttga ggaagcaatt gaaaaaagta tacaagcttt aaaagaacag
     1801 gatatagaat tggcagaaga agtaatggaa gaagatgacc aaattgatga atatgaatta
     1861 gagttagaag aaatgtgtac gagattaatt gcaacccaac aacctgttgc taaagattta
     1921 agaactatta ttgtcatttc taaattagct actgacttag aacgaattgg agaccatgct
     1981 tcaaatattg ctaaaatggt tattaaaata ggggatgaac ctctcctcaa acccttaatt
     2041 gatattccca gaatgactga tattgttacc cgcagattga aaaaaagcct ggaggctttt
     2101 gtgaatctag atgtggaatt ggcccatgaa atagccaaag aggacgaaga agtagatgtg
     2161 ttagatgaac agatattaag agaactactt acctttatga ttgaagaccc taaaactatt
     2221 agacaggcca tgtcattaat ctttataagt agattcttag aacggattgg tgaccatgct
     2281 accaatatct gtgaaagggt aatatatatg gctactggtg attgggatgt atcttattaa
     2341 ttagtttaac catttttgaa gggatatggt taagatgtca aataaaatct taattgtaga
     2401 tgatgaaaaa aatattcggg atttagttaa atacaattta gaagaaatag gatatgatat
     2461 attagaagct gaagatggtg agacagccat aaataaggca aaaaaagatg ttgatttagt
     2521 tattcttgat ttgatgcttc ctaaaattga tggtttagaa gtttgtaaaa gaataagaaa
     2581 taatgaaaaa ttaaaagata ctgcaattat aatgttaaca gctaaggatg aagagttaga
     2641 tagaatttta ggccttgaaa tcggggccga tgattatatg actaaaccat ttagtattag
     2701 agaattaagg tctcggatta aagctgtttt aagaagaaca aaaagtgatt ttaacaaagt
     2761 taaatcagat aatgataaat caaaatttgt aacgggagat ttagagttaa atatccaggg
     2821 ttatgaagta aagaagaaag gtagaacttt agagatgact cccaaagaat ttgaattatt
     2881 gagatttatg tttacaaacc aaaataaggt attaacaaga gaaattttat tagaaaaaat
     2941 ttggggatat gaatatacag gtgataccag aacagtagat gtacatatcc gcaggttgag
     3001 aaagaaaatt ggtagggaat atataaaaac tatcagaggt ttgggctata agcttgtata
     3061 ttctaagaaa taagttaaaa aaagtttttt ataaaaataa tactcctttt aacaaattat
     3121 taacctattt gtaaactttt attcatttgg ttgtaacaat cacctgttat tatattattg
     3181 aagtcaacaa aatgaatata atttcaagga ggagttaatt aatgagaaag agtttactta
     3241 ttttactttc tggattattg gtaatggcat tattttctgg cgtggtagga gcacaaaaca
     3301 atttaactat tcagggatct tccactgtac ttccgatagc tcaaaggatt gctgaagtat
     3361 atatgcaaaa caatgaagac gttaatattt ctgtcagagg tggcggttct ggtaatggta
     3421 ttgcagcttt aattgatggt acaactgata tagctgatgc atcaagattt attaaacaaa
     3481 gtgaagtaga aaatgctgta aataatggtt attatcctgt accacaccgt gtagcaatgg
     3541 atggaattgc agtagttata aatcctgcta acccggtaga atctttaacc ttagatgaaa
     3601 t
//
LOCUS       DATGCJ010000008         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_90181, whole genome shotgun sequence.
ACCESSION   DATGCJ010000008 DATGCJ010000000
VERSION     DATGCJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90181"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VKN64_00255"
     CDS             <1..295
                     /locus_tag="VKN64_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58692.1"
                     /translation="WLNEGISLYEAKQINPKQIKKLIKADRVPTITQLERNFYENFGY
                     TISGTIVQYIIDAYDYEKLIKFIKSPTDYEKIFGLTKKGFFTNWKGYLYNKYQ"
     gene            complement(388..2166)
                     /locus_tag="VKN64_00260"
     CDS             complement(388..2166)
                     /locus_tag="VKN64_00260"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HMA58693.1"
                     /translation="MKNKLLKNRQQFFMILALILLCSVLSFYNFNLFHIVITIYSIVI
                     CFIIFIIVLNTHQIVSNNYYIFLGMAFGFVGLFEFIHTLSYLGIEVLPILTGENTAQI
                     WYLGRLTQALALLVSFFFTNKKLNIKWLITTFSIYTILFLSAIIYWEIPVFSYLPVIN
                     VRGYQIQNTYLIIAILGITLIVLTRRKLTFSDFHYNNMASAIIFFIISELFFMLPAAT
                     YDFSNVFGHITRLLSFYFIYRSAVLSGLKEPYNTIFYKLTQSNKKLNELNQILSIVKN
                     IHETINVNLELNTLFEKIVNILIKKEDYQMAFIGEYLEESSEIKIRSSVGISSDFLKK
                     EKLDLNKDDNAVVEAIKRRRIIKDNNPQSVLNIFDRTDNKIHNSLIALPISYDNNLYG
                     VMVITSYREDAFNDRVVELLNKITQNLGIAITRYFTQQQIRYMSFHDQLTGLYNRNFF
                     NEEINRLDTSRKLPISIIITDFNDLKYINDSYGHKIGDKFLKTYAQILKRNSRQEDII
                     ARWGGDEFVILLPNTDENLTEKLLERLKSAVENVEIKGEKMSIAFGYAIKSSPNQDIE
                     NVFRQADQNMYQNKKLMKNNYRENQM"
BASE COUNT          764 a          337 c          355 g          741 t
ORIGIN      
        1 ctggttgaat gaagggattt cactatatga agcaaaacag atcaacccca agcagatcaa
       61 aaagttaatt aaggcggacc gggttccgac cataacccaa ttggaacgta atttttatga
      121 aaactttggt tatacaattt caggtacgat tgttcaatat ataatagatg cctatgatta
      181 cgagaagttg attaaattta ttaaatctcc taccgattat gaaaaaatat ttggtttaac
      241 aaagaaagga ttttttacta attggaaagg atatttatac aataaatatc aataaactat
      301 atcctgatat tttagaaaaa ttagaagaca tttaaatgcc gagttaaaaa agaatattga
      361 gcatagaaat agggaaaatc gaatggtcta catttgattt tccctataat tatttttcat
      421 gagcttttta ttttgataca tgttttgatc ggcctgtcta aaaacatttt caatatcctg
      481 attgggcgat gatttaatag catagccaaa ggctatactc atcttctcac ctttgatttc
      541 cacattttca actgcacttt ttaatctctc caaaagtttt tccgttaagt tctcatctgt
      601 attgggtaaa agtattacaa attcatctcc accccagcgg gcaataatat cttcctggcg
      661 ggagtttctt tttaaaattt gggcataggt ttttaaaaac ttatcaccta ttttatgacc
      721 atagctgtca ttgatatatt ttaagtcatt gaagtccgtg ataataatac tgatcggtaa
      781 cttacgggaa gtatccaacc tattaatttc ctcattgaaa aagtttctat tataaaggcc
      841 ggtcagctga tcgtgaaaag acatataacg gatctgctgt tgtgtaaagt atctagtgat
      901 tgcgattccc aagttttgcg tgatcttatt caataattct acaaccctat cattaaaagc
      961 atcttcccgg taagaagtta ttaccatcac cccgtataaa ttattatcat aactaatcgg
     1021 aagggcaatt aaggaattat gtatcttatt gtctgttcta tcaaaaatat ttaggactga
     1081 ttggggatta ttatctttga taatccttct tcttttaatg gcttcaacta ccgcattatc
     1141 atctttattt aaatccagct tttccttttt taaaaagtca ctcgagatac ccacagaaga
     1201 tctgatcttt atttcagaac tttcttccag atattcccct ataaaggcca tctggtagtc
     1261 ctctttttta attaaaatat tgactatctt ttcaaaaaga gtgtttaatt ccaaattaac
     1321 attgatagtt tcgtggatat ttttaacgat acttaagatc tggtttagtt cgtttaactt
     1381 cttattggac tgggtcagct tataaaaaat tgtattgtaa ggttccttaa gtccgcttaa
     1441 aacagctgaa cggtaaataa agtaaaatga taaaagcctt gttatgtgcc cgaagacatt
     1501 ggaaaaatca taggtagcag ccggtagcat aaagaaaagt tcggaaatga taaaaaagat
     1561 tatggctgag gccatattat tataatgaaa gtcactaaag gttagttttc ttctagttaa
     1621 gacaatcaaa gtgatgccta gtatggcaat tattagatag gtattttgaa tttgataacc
     1681 tcgtacatta atgactggta agtagctgaa tacaggaatt tcccaataaa taattgcact
     1741 taaaaagagg atagtataga tagaaaaagt agtaattaac catttaatat ttagcttttt
     1801 attagtaaag aaaaaagaaa ctaataaagc taaggcctga gtaagccttc ctaaatacca
     1861 gatctgagct gtattttcgc ccgtcaaaat aggtaaaact tcgattccaa ggtaggagag
     1921 ggtatggata aattcaaaaa gaccgacaaa cccaaaagcc atccccagga atatataata
     1981 gttattagac acgatctggt gggtattgag tactataata aagatgataa aacagatgac
     2041 tatactataa atagtgatta caatatggaa taaattaaag ttatagaaac tcaatactga
     2101 gcataatagt attagggcta atatcataaa aaattgttgt ctgtttttaa gtagtttatt
     2161 cttcatttta aacctccgtg tttaataatt ctatata
//
LOCUS       DATGCJ010000009        38954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_268689, whole genome shotgun sequence.
ACCESSION   DATGCJ010000009 DATGCJ010000000
VERSION     DATGCJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 38954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38954
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268689"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VKN64_00265"
     CDS             <1..1443
                     /locus_tag="VKN64_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMA58694.1"
                     /translation="SVGSAYAIHIVARYYEGIEEGYEKDRAVKLSIKNVGIAVIMAGG
                     TTMIGFASLFFSDLVIIQDFALGTTIGVGIALLVSIIFIPAVLINLKVTKKSRNGKEL
                     KITQNIFKGIESLVFKYRKPLLVFVTIIIVGSIIVIPSIKTESNYITYFKKGSTTRVS
                     TNLVDERFGGSQPVEVVIEGNIKNPELLKSMRDFQEEAVKLSYINNAQSMVNVFREEN
                     MALNNIREIPSSQNQIAQYLLLLEMSDKELISNYLTFDYQKNRIQMTMEMVSSTKQKE
                     VLNELEKLTNKYFGEKFKAEITGTPSLSIEINNRIISGQIKSLISAVLFTFVITSLLL
                     KSIKRGFFTTLPIAFTVLVNFGLMGMLNIKLDIATAMIASIAVGIGVDYSIHIFTRFI
                     EENEDLDSSIEALKTSIQTVGRANLYNASAVIVGFIVILLSSFPPLVRFGGLTAFTMV
                     VSFFGALVLLPVLILSAYKISGDKDLLDNN"
     gene            1462..2235
                     /locus_tag="VKN64_00270"
     CDS             1462..2235
                     /locus_tag="VKN64_00270"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HMA58695.1"
                     /translation="MNKRLSIILSVIVVGSLVLTSPVVLAVTGEEILDEVDKTLDSQT
                     RRSEIKMTIVNENGQKRERAVEIISKGDNKGFVEFLSPADVEGTSFLTLTEDGQENMW
                     LYLPAIGNVRKIASHMRSGSFVGTDFSYNDISMVGGSDYKSEYNSTLKEEVEYDNRTC
                     YLLESTPTKEDIQYSKMKMWVNKANYMPLKIEFYDSEGELLKVMTNEDIKEIDGHLTP
                     QKITMKNVQAGTQTILKLSNVEYNIEIPDKIFTTRYMQQ"
     gene            2270..3568
                     /locus_tag="VKN64_00275"
     CDS             2270..3568
                     /locus_tag="VKN64_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HMA58696.1"
                     /translation="MFKKIAVLFVISLFLVTSSAIALTESLGGKLEQDIIYNNAGEEW
                     QGKSLIELELDKSFGFDKKLYINSEIYTNYLNEKFTDLKIDFNEAYFDLYLKNSDLTI
                     GKQMINWGSAYEINPSSMTNPYDLTALNPTEEKLGVPAIRNTYYFNNRTSLTGVVITD
                     FVKSPVPASLKENMIKGIKADFAEQLTKQGVTNPNLILNNNLEVVESEPEIDEIGELE
                     YAVNLMKRNVRGFDLSASYFNGYEDIPSITSDLDNVKQDLKQIVGEYQRNNYSDTTTA
                     LDIDYKRTQGIGLNAIGSISSVGVWSELNYQINEDDEKTIDLILGGDYTFDNKLYTVA
                     QFIHRGYSDHQIPRESMNYLLLHGDKPFKQIHTIKANLIYDLGLGNYMFNPEMTFSLS
                     NNINLDLGTVITGEKEGTDGYALFNMMSGERTYFELTYNF"
     gene            3705..4148
                     /locus_tag="VKN64_00280"
     CDS             3705..4148
                     /locus_tag="VKN64_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HMA58697.1"
                     /translation="MDRPNWDQYFMEMAELASKRSTCLRRKVGAVLVKDRRILATGYN
                     GAPKKLPHCEETGCLREQKNVPSGQRHEICRGIHAEQNLIAQSAVHGVKTEGATVYCT
                     NQPCSICTKLLINAGIKKIYYKNPYNDEFTKKLLDQSEIEYKVIK"
     gene            4258..4671
                     /locus_tag="VKN64_00285"
     CDS             4258..4671
                     /locus_tag="VKN64_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMA58698.1"
                     /translation="MNVKVDLMENLKFNAQGESGHDMPMDASESAGGDDSAARPMEML
                     LAGLGGCTSIDVVLILNKMKAEIEDFNVDIEAEREDEHPKRFKKIHLNYYIKGKNLDK
                     KKVEKAINLSENKYCSASASLNAEITSSYEIENIE"
     gene            4923..6398
                     /locus_tag="VKN64_00290"
     CDS             4923..6398
                     /locus_tag="VKN64_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMA58699.1"
                     /translation="MIDLEINEDQLKRTVQRAREKNIIMPTFEQMKNPELIPDEIKEK
                     LKDIGLWDINSYNLFRITWKNEPKKEGGLYDGVNYLELPSKLTGVDARIIGVVGKWFP
                     TGAHKVGATYGCLAPRLITGQFDPTYHKAIWPSTGNYCRGGAYNSELLACDSIAILPE
                     EMSQERFEWLSNVAGEVIATPGSESNVKEIFDKTWELKAERDNVVAFNQFEEFGNHLW
                     HYEVTGSALEEVLEKEMGEDENYRGVTLTSGSAGTLGCGDYLKEKYPTSKIAVGEALQ
                     CPTLLNNGFGAHRIEGIGDKHVPWIHNVKNTDMVIAVDDEDTMSLVRLFNEPAGHQYL
                     REELGLEQDFIDKLKLMGISCIANMIMAIKFAKYYELGEDDIVLTVFTDSMELYQSRL
                     KEMEEEFGEYTKTDAAVDYNAHLSRLSTDYLEELTYESRKRIHNLKYYTWIEQQGREL
                     EELNAQWYDYENYWGEIHNMTPKIDELIKEFNERVGLLEEL"
     gene            6422..7672
                     /locus_tag="VKN64_00295"
     CDS             6422..7672
                     /locus_tag="VKN64_00295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMA58700.1"
                     /translation="MKYVKNLKCTNCGETYEAVPDRYLCDKCGDKGILDVQYKYEEIK
                     KDWNKEKLANNSESSIWRYLPLLPIEEDTKRPNLRIGNTPIYNSKEMGKKLGLNNLII
                     KDDGLNPTGSLKDRASAMAAVKAREAGAKTVACSSTGNAASSLAGNIASMEDEMNAII
                     FVPERAPSGKVAQLLVYGAEVVSVQDSYEAAYYLSDEAIKKWGWYNRNAAINPYLVEG
                     KKTSVLEMAEQLNWEMPDWVVFSVGDGCTIAGAYKGLYDLKQIGFIDRIPRLLGVQAA
                     GCAPITKAFRTGKDLVVTDENTIADSIAVGSPRNYRKALNAVKNSKGEYINVSDEEIL
                     NMIKLLGSTTGIFGEPAGVAGLAGIKNAVKEGIIGKEESVAVVITGNGLKDIDNARKA
                     AGELIEIEADIDKLAEIFDKKGVI"
     gene            7672..8226
                     /locus_tag="VKN64_00300"
     CDS             7672..8226
                     /locus_tag="VKN64_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA58701.1"
                     /translation="MVEANMKMGEKIRNIRKSQSLSLSELADKIGKTSSYLSQVERGL
                     AEPSITALREISKALNIPIFYFLIEDQKHNAVVRKEDRKVLRFPGSHLTFELISPDLE
                     HDIEMIEAKLEPGASTLDKPLSHNGEEVSIVIQGKMEITIGSERYELDEGDSIYYNAN
                     LPHKITSIGKEELIFISAITPPNF"
     gene            8260..9501
                     /locus_tag="VKN64_00305"
     CDS             8260..9501
                     /locus_tag="VKN64_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HMA58702.1"
                     /translation="MKEFSEILKQAEKYRPEISKFLRDMIAIPSESCQEKEVILRIKE
                     EMEKVGFDKVEIDDMGNVLGYIGHGDHLIAMDAHIDTVGLGDKELWDFDPLEGYEDKE
                     VIGGRGASDQEGGMASMVYAGKIIKDLDLEDDYTLVVTGTVQEEDCDGLCWHYMIEEE
                     NIRPEFVVITEPSSCNIYRGHRGRMEIKVTTHGVSCHGSAPSRGDNAIYKMAPIINEL
                     KALNENLKYDEFLGKGTLTVSEIFFSSPSRCAVADGCSISIDRRLTAGESWEFALQQI
                     RNLPSVKEANADVEMYEYERPSYTGLSYPIESYFPTWVIEEDHPVCSSLVDSYQKLFD
                     DEPLVDKWTFSTNGVSIMGRHGIPCIGFGPGHEDQAHAPNEKTWKDELVKAAAFYALI
                     PTVYVNDYADKMPEVTKNIVE"
     gene            9523..10506
                     /locus_tag="VKN64_00310"
     CDS             9523..10506
                     /locus_tag="VKN64_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMA58703.1"
                     /translation="MKTEFRGKDFITLQDWSKEELDTLLEVSFDLKKKFAMGLDTPYL
                     NNKTAMLMFFEQSTRTRNSMEAGLAQLGGHANFLDTSTMQVSHGEVAKDTAVILGRFG
                     HMIACRNCEWGIGNDYLTSLAKWAPVPVLSLQDDLYHPMQGIADLMTIMEKRENTRNL
                     KVSIIWAYATSHKKPISVPVTQSLLFPRFGMDVTLAYPEGYELPDWVIEQSRANAEKH
                     GGSFKITHDMEVAYTDADVVIPKNWGSWATSQSDDVIDENLESNKEWKCTEEMMAMAD
                     EDVMYMHALPADRGNEVEDSVIDGPQSIVYDEAENRLHTAKAVMTLTMGGK"
     gene            10893..11117
                     /locus_tag="VKN64_00315"
     CDS             10893..11117
                     /locus_tag="VKN64_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RRXRR domain-containing protein"
                     /protein_id="HMA58704.1"
                     /translation="MRKLLNYVYVIAKVDRPLINTKKFGIIRPFLDEDKAVSLKTKPF
                     TIQFSYETGHHIKDITVGVDAGYQNIGYLL"
     gene            11556..12893
                     /locus_tag="VKN64_00320"
     CDS             11556..12893
                     /locus_tag="VKN64_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HMA58705.1"
                     /translation="MSENVNKREETEAYYKLEDRPPTGETILLGFQHMLAMFVGIITP
                     PLIIAGAAGLNAAESAFFVNMALIISGVASYIQCHKFGPVGSGLLGVHGTSFTFVPMA
                     ISAVNSGGLALVLGMALAASPVEMIISRFIKQTKKFFPPVVTGVVVMLIGLGLMETGM
                     TNFGGGAGAEDFGSVQNLLLGSFVLLIIVFANRFGKGLVKVGAVAIGLVVGYLVSIPL
                     GMVDFQPVAEAGWFTVPIPFKFGMSFRWSHYLPWILAYVITSIESIGDLTAISEVSGE
                     PVEGDLYRERISGGILADGIGSALAAVFNTMPNTTFSQNVGVIQMTKVGSRVVGYAVA
                     GILVILGLLPKIGALISVMPNAVLGGATIALFGMVATSGMKIVVRGGLTDRKIFILSV
                     ALSIGLGVTFRPDIVAKLPDILSTILSSGITIGAVSAILLNLLLPRKEEPVEC"
     gene            13049..15346
                     /locus_tag="VKN64_00325"
     CDS             13049..15346
                     /locus_tag="VKN64_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HMA58706.1"
                     /translation="MEFKHVNKSEKRVDVYEKVTGKAKYGADMRFPDMLYGKVLRTEY
                     PYAEIKNIDTSKAEEMPGVVAVITAADIPNNEFGVIIENQQVLAKEKALYIGDGIAVV
                     AAETAKQAAKAAEKIEVEYEELEGIYDPLKSREEDAQLVHEELEDNQVIHHPLRKGDI
                     KEGFEKSDVIIEREYDTPFIEQSYIEPEAVIAVPYQENSTVTVYGSVQNPFATQNAVA
                     TVLKEDLNKVRIVQNHMGGSFGGKDEVVSSMAARASVLALKTERPIKLVNTRDESIKE
                     SYKRHPYHMKYKIGATKDGKIQAMEIEAVADSGAYACQTPFVTWRSVVQATGPYEVDN
                     VKTDTYGYYTNNVYTGAMRGYGSPQVIFAQESLMDELAEELEMDPVELRMKNIYHNNS
                     FTASGQKLDNHEVSLEEVLTKAVEKSNFIEKYKEYSKEQPGDKKKGIGLSISFRGCSL
                     GAEAIDAAGIILSLKKDGSVSLYSGLAENGQGLKTVYCQMAAEALGLDTEKINFMQVD
                     TLISPDSGSTVASRATLIGGNAVKKAADNLIDKIKEYISDKYDQAKEDIYLKDGSIYG
                     EKDVEIMTVNEMAAKAAMEGVYLTTYGWYVSPDISWDEETGHGRPYFTYVYGCQVAEV
                     EVDTATGEIDVLNMTAAHDVGRAINPANVKGQIYGGVSMGLGYGVMEEIETDEGYIEN
                     TNFDEYMIPTVKDMPDIDPIIVENPDPNGPFGAKSIGEPTLELGAAAIANAVANATKK
                     RIRSLPLNLERIIVGKDLHKGVDHK"
     gene            15343..16212
                     /locus_tag="VKN64_00330"
     CDS             15343..16212
                     /locus_tag="VKN64_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HMA58707.1"
                     /translation="MIEFEALKANSLEEALDILAEKKKEVRMVAGGTDMLVQIHHESD
                     DLKGMEYLLDISSIPGLDLIEDNNEYLKIGALCTHSEIMDSVLVNEKFPILKKAAEVI
                     GSTQIRNRGTVGGNINNASPAADLVTALIALDASVVLHSKHGKRELSLSEFITGPYMT
                     DRYEDEILTEVKVPYIQGEYFDCYQKVKRRQAVAIARINLAVITKLKDNKFDDIRIAF
                     GSATPTAMRFREIEAELEGSDLNKIDYDKLADETGQAMVNITGERWSTPYKRPVVGNL
                     LKRALQSIVKEVQ"
     gene            16215..16691
                     /locus_tag="VKN64_00335"
     CDS             16215..16691
                     /locus_tag="VKN64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA58708.1"
                     /translation="MEEVNVTLTVNGKEHDLNVAADERLLDTLRDRLHLTGVKEGCSI
                     GECGACTVILDGKAVNSCMVYTFQIDGSEVLTVEGLETNGKLDPLQEAFIDHQAVQCG
                     FCTPGMLMSAKALLKENPDPDREEIKTAIEGNLCRCTGYQQIVDAIESVVKKDGSE"
     gene            16694..18061
                     /locus_tag="VKN64_00340"
     CDS             16694..18061
                     /locus_tag="VKN64_00340"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HMA58709.1"
                     /translation="MSKILIKDIKELITMDDERKRYKDIDLLIEDEQIKAIDENIDIS
                     AAEVDEIIEAGNYFAFPGLINTHHHFYQTLTRNIPAAQGVELFNWLKFLYPIWSKLTP
                     EAVYYSTLLASAELLKTGCTTSVDHFYVFPDSQDDNLMDYQFKAAKEIGIRFHGSRGS
                     MSLSEEDGGLPPASVVQSEDEILNDSQRVIDKYHDESEYGMQRVVLAPCSPFSVSKDL
                     MIESIKLARQNNVLSHTHLAETLDENQYCVDTFGKRPLKLMEEWDWIGKDVWFAHGVH
                     FNEFEIAKLAETGTGVSHCPASNMKLSSGAAKIPYMLKKQVPVSLAVDGSASNDASNM
                     ILELKLAYLLHKLIHGIKSITSEDVLSIATNGGRDVLNQPEIGSIEEGKAADLFMVRS
                     DRLGFAGGQSDPVEALVNTGTTQEVDYNIVNGKVVVRKGELTTIKEYNVIKKHNQIAQ
                     DLLSE"
     gene            18074..19405
                     /gene="ssnA"
                     /locus_tag="VKN64_00345"
     CDS             18074..19405
                     /gene="ssnA"
                     /locus_tag="VKN64_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HMA58710.1"
                     /translation="MRLYGNGILITLNSQDPVINDGAVLIDKGIIKDYGKTDKMKVRY
                     QEAEFENVEGKVIMPGMINSHMHFYSTFARGMDLKTEIPPQNFTEILEKLWWRLDETL
                     NSEDIYYSTLYALIESIRSGTTTIFDHHASYGYIENSLDIISEAVIKSKLRTSLSYEI
                     SDRHGEQKTDQAIKENERFINRAKSLNSDFITPAIGLHASFTLSDETLKEVADLAEST
                     NTPTHFHAAEGKVDEEDSKKRGYKNLADRFDKLNILREDSLAIHGVHLSEEEYKILAD
                     NGVYLIHNPESNMGNAVGAINLRKALDKNLVIGLGTDGYTTDMFESINVADLLQKHDN
                     SNPTLGGEDIKKIAFANNRKIAKSYFGKELGVIKKNTPADIIIVDYKAPTPINDENYF
                     YHILFGFNGGLVDTTIVNGHVLMENQEVKVVDYEKTIERCKKQAKDFWRRF"
     gene            19347..20252
                     /locus_tag="VKN64_00350"
     CDS             19347..20252
                     /locus_tag="VKN64_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HMA58711.1"
                     /translation="MKKQSKDVKNRQKTSGEDSSSYFRPKSKEGLLQILRDYEEDFTI
                     IAGGTDLLVEHYQNLYKIDRWLDLNWVSEFKEIKIYDDKVEIGSMVTHERLYKLDKIK
                     ELYPVIQQAAADVGSPQIRSRGTIGGNIVTSSPAGDLLPPLLVYDAKLKLISKEGERV
                     VDANEFFTGPKRNVMENGEIVEKIILPLRDEKYKGAWVKVGKRKALVISSISLALNLV
                     FDEDEKIKESRICIGSAAPVPLRIESAEEQLLDKKASKVKIDEVAQIVADEISPIDDI
                     RGTAKYRRQVAFNITKSALSEVINQ"
     gene            20249..20731
                     /locus_tag="VKN64_00355"
     CDS             20249..20731
                     /locus_tag="VKN64_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA58712.1"
                     /translation="MKITLTVNGKKREVEVKPHTRLLDVLREDLGLTGTKEGCGKGEC
                     GACTVIMDNKVVASCLVLAPQADGSEIQTVEGLAGKDKLHPIQQSFIETGAVQCGFCI
                     PGMIMSSKKLLDEKINPSEEEIKRGLSGNICRCTGYVKIIDAVKEASHKMQSGGEENV
                     "
     gene            20724..22043
                     /locus_tag="VKN64_00360"
     CDS             20724..22043
                     /locus_tag="VKN64_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HMA58713.1"
                     /translation="MSKRYVGEDILKVDARDKVTGQAHYPSDLKFDDYLHVKIKRATH
                     PHAILKDLDISKAMQVEGVVDIITEADYPDLNKFGLIIKDQPVLIKIGEKMRFMGDAL
                     AIIVAETAEAALKAREKIEVEVEELEVISDPHRAIEKDAPAIHDDLDDLCTEYLSPDV
                     NRKNILVDHHLEKGDVDKGFEQADFIVKNDYQTQHVDQLPLQNEAGYAVYDEKSGEIK
                     IWSASQWLHDTQADVAQSLNMSKDKIMIINAAIGGAFGKKEDVSVHIHLALAAMRVKR
                     PVKLNYTREESMISQSKRHPLTIRAKTGVTNDGYLTAWQVFVIGDTGAYASSGPAVVH
                     KGMYHCTGPYNVANVRGTSYAVYTNNTYGGAMRGFGTTQMAFAYESQMDIIAHKLNMD
                     PAQFRYQNAYDTGSTTPNGQMLGKSVNTKRTIEEAINLSKWEGEINK"
     gene            22040..23029
                     /locus_tag="VKN64_00365"
     CDS             22040..23029
                     /locus_tag="VKN64_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HMA58714.1"
                     /translation="MRKKGRGMATIMFGFGYGEGFPDHSIAEVEITPDKRIKLRTAGA
                     DVGQGFLTTMTQIAAEVLKIDTSYIEIVGQDTHSAKNSGSTSATRQTFFSGNAVREAA
                     EEVMGKIYHHGSLHFNSNHPELTVKDGELYACNEENKITYFELAEMVEEKGEKLSAEA
                     SFFPKTYAPDKKTGQTEKNYVAYTFMTQVMDIEVDTDTGIIEVMNVYTALDVGKAINP
                     QNVHGQIEGGTVQGLGMALMEEQVIDEGITLNADMTNYLVPSIKDIPEFESTLIENKD
                     SDGPFGSKGVGEPTLIAANPAVANAVYDAIGVRIKSLPITPEKILEALEEQKS"
     gene            23029..23607
                     /locus_tag="VKN64_00370"
     CDS             23029..23607
                     /locus_tag="VKN64_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HMA58715.1"
                     /translation="MPNNILITGDKYVGKSTIVKRTLDKLNLKPGGFVVGRTGNLDQW
                     LSFYLTDPLDYYYHWQGKSSSKYKKKCQIFAKRDDPSQDWEIQPDVFDEKGVQFLETG
                     RNFRDVVVMDELGRFELEADKFQAKVFEILNSNKPVLAVIKDEHNKFLDKIRDREDIS
                     MYRVKEKNHETVFEDVYKELRSIILEINKNKE"
     gene            23613..24701
                     /locus_tag="VKN64_00375"
     CDS             23613..24701
                     /locus_tag="VKN64_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA58716.1"
                     /translation="MADLQVEFAGIKLDNPVMPAAGPPVKDAKAAKRAKNGGAGAIVT
                     KTISTTAAKVPRPNMARIKGGFLNTELWSELTPEHWLEEEYPKIRELDLPVIAGIGYT
                     KDQIRELAKKVEKYADALELSTHYLGDNSTPMEESIKAAKDEVDIPVFVKLSPGIDIP
                     LFAKAAEEAGADGIVLINSFGPTLDIDPQTGKPLMGSDNGFGLLSGEAIFPLALRAVY
                     QASSNVDIPVIGVGGIDSGLRAAKMFMAGASAVQVCTEAIVNGPSVYKKINEELNKFL
                     DENGYESVNDIKGLTAQVVKEEANFNTIVPTVIEENCTTCGLCITSCVYDAISYNESR
                     DYIVIDPDKCAGCGLCITRCNFGALKLE"
     gene            24747..26462
                     /gene="ade"
                     /locus_tag="VKN64_00380"
     CDS             24747..26462
                     /gene="ade"
                     /locus_tag="VKN64_00380"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HMA58717.1"
                     /translation="MVNDKILKMAKGEIPAELVLKHGRIVDVFNERLIREDIAIADGK
                     IIGIGDYEGVKEIDFEENIIAPGFIDGHLHFESAMVRVDEFAAWIIPRGTTTVFADPH
                     EIANVAGLDGIKYFLDRGTELPWNFNLMLPSCVPATANETSGAVLTADKLEKLKEAEG
                     IFGLGEVMDFVGVIKGDNKIWDKIDMIGDKFIDGHAPGVSGKNLNAYLLGGIMADHEC
                     TEPEEALEKVTKGMYVMIREGSVTRDLVSLLPAVNNSNLSRFLFATDDRDPEDLYENG
                     HMNFVIKKAIQNGISPLRAIKLATINSANALDLKNVGAIAPGYKADLVILDNLKDFNV
                     EKVFKDGQLVAENESLIVNLPEIKEGKSEKIFKSVNIGKLKKADFDLPDFKEYRVMSL
                     IKDQIVTGKETIKLENEVKVDDLVANNLVKIAVVERHKKTGNVGLGLLKGFGLRKGAI
                     AISIGHDSHNIIVVGLNSQDMYRAVKEIERLQGGIAVVESGQLIDCLSLEIAGLMSKD
                     SIPEVAKKLRKMKEKVYSMGVTRQSPFMTLSFMALPVIPSLKITDQGLFDIDENKFVS
                     LGVDD"
     gene            26555..27877
                     /locus_tag="VKN64_00385"
     CDS             26555..27877
                     /locus_tag="VKN64_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HMA58718.1"
                     /translation="MNEVNEGGFLDRVFKLKENNTNPKTEVIAGFTTFLTMAYIIFVN
                     PSILSEAGMPFNGVFIATIAGIILGTLAMALLTNYPFALASGMGLNAFFAYSVVIGMG
                     VSWQTALGIIFLEGIIFIVLSVTPVRKKIVNAIPMNLKTGISAGIGLFIAFIGLQNAG
                     IVVNDEATLVSMGSLLSGPALVALVGLIVTGILHAMKVKGSLLWGILIATAFGWTNGV
                     TPAFNGIFAMPKMADWNQVLFQLDIASALRLSMVGVLLSFLFVDLFDTAGTLVGVSKQ
                     AGYIDEDGNLPKASNALLADAIGTTGGALFGTSTVTTYIESAAGVAEGGRTGLTGVVA
                     AILFFLALFFRPLIGLVPGAATAPALIIVGTMMMTNVTDLDWDDFTEVLPAFIAMIAM
                     PLTYSISNGIALGFIIYPLVKLFTGRGKEVNALVYVLGAVFIAYFLWI"
     gene            27938..28540
                     /locus_tag="VKN64_00390"
     CDS             27938..28540
                     /locus_tag="VKN64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMA58719.1"
                     /translation="MYTAIILAAGEADRMGKLKQLLPWQDKTILETVIDNVLASEHID
                     DEVRVVLGAEAERVKNKIDYYDDPRFRIKENPDYSRGMSSSLRVGVRNLSKNTKSLVI
                     FLGDQPLITPDIFDNIIIEFEKSSSEIMMPIYNEKPGHPVIISTKYLDEIEKLEGPMG
                     LKPFIEEHKDLVNYYPIEEEKVTIDLDYYDDYIYYKNKYN"
     gene            28551..28907
                     /locus_tag="VKN64_00395"
     CDS             28551..28907
                     /locus_tag="VKN64_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMA58720.1"
                     /translation="MKLNYKLWLEEDKERLFGDGPADILRLVDRLGSLSKAAAEINMS
                     YSQAWQLIDKLENKLGFKLLEKQVGGTEGGGSTLTKKGRLLMDTFLSFRTEARREMSK
                     LETKYFTKEFKDKLKE"
     gene            28971..29978
                     /locus_tag="VKN64_00400"
     CDS             28971..29978
                     /locus_tag="VKN64_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA58721.1"
                     /translation="MKKFTSKITIILTLILVVGLMASSSVLASKMVTDMAGREVEVPD
                     QVNRVVTTYRSATQFVFCLGVQDRLVASDLSFEKISLFQKLYEGEKLPNVGSKRHGLN
                     LEQIIETDPDLVILFPYNDGPEVAEKLKEFNIASIIIVPESYEQIKETTRILGKALGV
                     EERAQQVINQYDKILDIVDKKDSIEEKKKVYFANSDLFDTVGKNMLQTSMIELAGGLN
                     PAKEVKSGFIKTSLEELIKWNPEVIVVSQYYNGTVEDLLARPELQQLKAVKNENVYRF
                     PSKVEPWDFPSPSSYLGIVWLAEKLYPEKYSDLKLEAVVNEYYNTLYEKSYKELGGSN
                     S"
     gene            29996..30763
                     /locus_tag="VKN64_00405"
     CDS             29996..30763
                     /locus_tag="VKN64_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA58722.1"
                     /translation="MLEVKNLGFSYKKVKTLNNINFRVRRREFSCLLGANGSGKTTIL
                     KCLNGVLDKDEGKVKIDQTDLDNLNQRQIAQYISMVPQEHSVVFSYRVLDVVTMGATP
                     YLGFGKMPGQKHYDRAFQILAKLDIEHLADRQYNQLSGGEKQLVLIARALMQDTGYLI
                     MDEPTSHLDFKNKNLLMREIKKLTRNSETGVIMALHDPNLAMKYCDRVIIVKEGAIMA
                     DGDANEILTGQNLGDAYGIDIKINEKARGIEIALPEI"
     gene            30791..31474
                     /locus_tag="VKN64_00410"
     CDS             30791..31474
                     /locus_tag="VKN64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HMA58723.1"
                     /translation="MSYKKSIISGFKKSIPVILGYLPIGISYGILALQTDLSPYLIVG
                     MSVFVFAGSSQLIAVNMLTAQAALIPVIMTTFLVNLRHILMSASLSLHFKKTPQKLLP
                     FIGFVITDESFALGNTLLNDREENKGIFFLSMGLSAYLGWVLSSLAGVIIGRYILNFE
                     IPVIDFVLPAMFIILLIMQINEKMDIVVSIMAAVLSIIFYFILPGNWNIIVATVTAAV
                     AGVIIENAI"
     gene            31464..31781
                     /locus_tag="VKN64_00415"
     CDS             31464..31781
                     /locus_tag="VKN64_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HMA58724.1"
                     /translation="MQSKYILMILGMTLVTYIPRMLPVTLLSKVKIPDTMVRILKFVG
                     PAILASLLAPALFITEGSVDISLHNSYLLAAIPTFFTAYFTRSIFTTVFLGMLYLYIF
                     TLI"
     gene            31795..32817
                     /locus_tag="VKN64_00420"
     CDS             31795..32817
                     /locus_tag="VKN64_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HMA58725.1"
                     /translation="MDIKIDLFSDTGTRPSDEMRKFMAEAEVGDEQLFEDPSVNRLTA
                     MASELLGKEEAIYLPSGLMANQISFAVHCNPGDEVIMDQSAHPIHYEGGATAVISGAS
                     IRGLEGEKGIFQLEQFEDAVRTKDFHDPQSKVLSIEQTSNLGGGRIWPLDLINNLTER
                     AHELGLKTHMDGARLFNAVAATGISAEEYAANFDSVWFDLSKGLGAPVGSVLAGSQEF
                     INKARYWKQRLGGAMRQAGIIAAGGIYALENNLERLKDDNKNAKLLAARLAELDKVEI
                     DPSTVDTNIVIFKIKGESSAEFADKLLEAGLRVSVLHDKLRAVTHLDVDRNDILEAVE
                     IFKKKL"
     gene            32792..33028
                     /locus_tag="VKN64_00425"
     CDS             32792..33028
                     /locus_tag="VKN64_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58726.1"
                     /translation="MRFLRKSYKYNTNIYVGNFNDPVKTRQTTESLISSLISEVIISG
                     VNLGNYELFRAVQNADRQIYITTTYTNKHYIAST"
     gene            33065..33697
                     /locus_tag="VKN64_00430"
     CDS             33065..33697
                     /locus_tag="VKN64_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA58727.1"
                     /translation="MVILGIGLSDLLYKIIIGVQLFAPEAPPVPVIDIPLLSNIPIIA
                     AIFNQDVIVYFTYIAVFVAYYIFYHTRVGLETRAIGEDPKAADVVRINVTLKRYVATI
                     TGGMLMRMAGAYLPLVITGTYTTNISNGRGFMAIGIAIFASWRPARAYLGAFIFAAIE
                     VLSFQQQLEQSMFPYQFLLMLPFISVLISIWIFRKRVEFPAPLGKPYSRE"
     gene            complement(33782..34156)
                     /gene="crcB"
                     /locus_tag="VKN64_00435"
     CDS             complement(33782..34156)
                     /gene="crcB"
                     /locus_tag="VKN64_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMA58728.1"
                     /translation="MLKILYIGIGGFMGASSRYLLSKWIEIKWDSLIPNGTLVVNTVG
                     SFLIGFLMILFLEKVTGFSNLKILLTTGFLGAFTTFSTFSYETMVLLEEKSFLMAGIN
                     IFGNLTLGILAVLIGIIAGKAI"
     regulatory      complement(34213..34278)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            34426..35340
                     /locus_tag="VKN64_00440"
     CDS             34426..35340
                     /locus_tag="VKN64_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HMA58729.1"
                     /translation="MESILRYSTEVEKAIKEDKPIVALESTIIAHGFPYPDNIELAIE
                     LEEQAREAGVVPATIAIIDGKLKVGLTKEELEFIGKSDDIKKASLRDLPVLVSKKLNG
                     ATTVAATMQIAYLAGIDVFVTGGIGGVHRKAEETFDISADLRALSEFPVTVVCAGAKS
                     VLDLPKTKEVLESKGVPILGYKTEKFPAFYLRDSGLEVDYSVSNGKEVAEIVKNNMLI
                     ENRNGILVTVPIPKKDELNRSQFNKIIDELVREVEDENIRGKDITPYLLARIKEETEG
                     ESVKANISLVKNNLRAGCEIAKSVKDLR"
     gene            35342..36238
                     /locus_tag="VKN64_00445"
     CDS             35342..36238
                     /locus_tag="VKN64_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HMA58730.1"
                     /translation="MILVIGDINLDIIVNLREEINFDTDTQSEIYFAGGGSSANFSYW
                     LDYLKQPVRLVAKTGDDFIVDYLKEEFKDTNIDFINLTSQEKQTGRVVVLLSKAGERS
                     MITDRGANIDIKPSDIKEKLFKDVEQLHLGGYSFFGGANMEKTALRAIKIAKDKNLLI
                     SFDPSSYSGLKEYGIQKILNQTAGIDLCFPNIEEGQVLTDKKKPKEITHYLLNYYKNV
                     ILKVGKKGCMVGNQEEVSFIQQKRKLAEGDTTGAGDAFSAAFIAKYSETKNLKEAGDY
                     ANQIGFECVNKIGGRPYNREAR"
     gene            36242..37357
                     /locus_tag="VKN64_00450"
     CDS             36242..37357
                     /locus_tag="VKN64_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMA58731.1"
                     /translation="MEKRKLGKTGLEVSLIGFGGFHLLEIPAEKARSLLNNYIDMGGN
                     YIETAADYGDGESERKIGPVIEKRRDEVVLASKTGSRIKEGALRDVNRTLNNLHTDHL
                     DILFMHAVKSFEELETIFSDGGAYEAFLELKEAGKVDNLGISMHGQPDVLIKALNKDK
                     FDVVMATFNYFDKFNFPKLEDELLPLAQEKEVGIVLMKALADGFLWRSAETAFRYAFS
                     LPVSIVVTGINTEDMLEEDIGYANTYEPMSEEEKEELYKNAPELGNYVCRQCDECFDC
                     PVDIDIKEIFKLEGYYDRQMRDGKVRNPADFALRDRLRFWFEGEEKARSLYQELDVKA
                     DQCTECEKCLEYCPYDLPIMEKLDIAHYKLSGEDVLF"
     gene            complement(37432..38715)
                     /locus_tag="VKN64_00455"
     CDS             complement(37432..38715)
                     /locus_tag="VKN64_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA58732.1"
                     /translation="MTNKNFKEQKPIETPFYYGWLILFIAALGLFFSGPGQTYSISIF
                     INHYIEKFNWSRSLISSFYSIATLISGLILTFIGRYIDRLGHRKMTTVISVLFGLVCI
                     WMSFVFNRFMIFVGFFLLRLLGQGSMTLLPQTIVPHWFKQKRGLALSLLGVGGVISSS
                     LLPPLNNWLINTYSLNFTWRFWAFLLIFLMAPVGWFFIRNKPENIGLKPDGIEEEYTN
                     NITPKVHISEDPWTLKQSMGTRSFWFMNYCGVISALVNTGLTFHMVSIVNNIGYSPGF
                     AALILSITAMIKLPFTFIAGWLLDRLKVNIIKGINFLILSGAIILLLKADSKIALIIY
                     GLVHGTFMAFDAVSTGVVWPNYFGRENLGSIRGFAMTTMVIGSALGPLPFGVAYDYFG
                     GYQEIMIGILILPTLAVFASLFSPPPKYGFKSIKK"
BASE COUNT        13236 a         6076 c         8442 g        11200 t
ORIGIN      
        1 agtgtaggta gtgcttatgc aattcatatt gtggccaggt attatgaagg aatagaagaa
       61 ggatatgaaa aagatagagc ggttaaactg tcaataaaaa atgtgggtat tgccgtgatt
      121 atggccggtg gtactacaat gattggtttt gcttctcttt tcttctctga tttagtaatt
      181 attcaggatt ttgccctggg caccacaatt ggagtaggaa ttgctctctt agtatcaata
      241 atatttattc cggcagtgct gataaatctg aaagtaacta aaaaaagcag aaacgggaaa
      301 gagttaaaaa ttacacaaaa tatcttcaaa ggaattgaaa gtttagtttt taaatatagg
      361 aaacccctct tagtttttgt gacaataata atagttggtt caattatagt aatacccagt
      421 atcaagactg aatcgaatta tattacttat tttaagaagg gttctactac ccgggtttct
      481 accaatttag ttgatgaaag atttggcggc agccaaccgg tagaagtagt aattgaaggt
      541 aatattaaaa atccagaatt attaaaaagt atgagagatt ttcaagaaga agctgttaag
      601 ttatcctata tcaacaatgc ccaatcaatg gtaaatgtat ttagggaaga aaatatggcc
      661 ttaaataata ttagggaaat tcctagcagt caaaatcaga ttgctcagta tttactttta
      721 ttagagatgt cagataaaga actaatcagt aactatttaa cctttgatta tcagaaaaat
      781 cgaattcaaa tgacaatgga aatggttagt tctactaagc aaaaagaagt tttaaatgaa
      841 ttagaaaaat taacaaataa atattttggt gaaaaattta aggctgaaat aacaggaact
      901 cccagtttat ctatagagat aaataacagg attattagtg gacagataaa aagtttaatc
      961 tctgcagtac tgtttacctt tgtcataaca tcactcttgt taaaatcaat taaaagagga
     1021 ttttttacaa ccttaccgat tgcatttacg gttttagtta actttggttt aatgggaatg
     1081 ctgaatatta aattagatat agctactgct atgatagcta gtatagccgt tggtattggg
     1141 gtagattaca gtattcacat tttcacccgt tttatagaag aaaatgaaga tttggatagc
     1201 tccattgagg ctcttaaaac ttccatccaa acggtgggta gagccaatct ttataatgct
     1261 tcagctgtta tagttggatt tatcgttatc ctattatctt cgtttccacc cttagttaga
     1321 tttggagggt tgacagcttt tacaatggta gtatcattct ttggtgctct agtattatta
     1381 ccagttctta tactttcagc ctataaaatt tcaggtgata aagatctatt agacaataat
     1441 tgatatgagg aggagatagc gatgaataaa agattatcga taattctttc agtaatagta
     1501 gttgggtcat tggtcttaac ttcaccagta gtattagctg taaccggaga agaaatttta
     1561 gatgaagttg ataaaaccct ggattcgcaa acccgcagat cagaaatcaa gatgacaatc
     1621 gttaatgaga acggtcagaa aagggaacgc gctgtggaaa taatcagcaa gggagataac
     1681 aaaggttttg ttgaatttct atctcccgca gatgtggaag gaaccagctt tttgaccctg
     1741 accgaagacg gtcaggagaa catgtggctt tatctgcctg cgattgggaa tgttagaaag
     1801 atcgcttctc atatgaggag tggtagtttt gtaggaactg atttcagtta taatgatatc
     1861 agtatggtag ggggtagtga ttataaaagt gaatataact ctacattaaa agaagaagtt
     1921 gagtatgata atagaacatg ttatctgtta gaatctacac ctaccaaaga agatatccag
     1981 tattctaaaa tgaaaatgtg ggtaaataaa gcaaactata tgccccttaa aatagaattt
     2041 tatgacagtg agggagaatt attgaaagtg atgacaaatg aagatattaa agaaatagat
     2101 ggtcatctaa ctcctcaaaa aataacaatg aaaaatgttc aagcaggtac tcagaccata
     2161 ttaaaattga gtaatgtgga gtataatatt gaaataccgg acaaaatatt taccacgaga
     2221 tatatgcagc agtaattaaa cttaaaaaca agaaggaggg ttgctattaa tgttcaaaaa
     2281 gatagctgta ctttttgtga tatccctgtt tttagttact tcatctgcca tagccctaac
     2341 agaaagcttg gggggcaaat tagaacagga tattatatat aataatgcag gagaagaatg
     2401 gcagggaaag agcctaatag aattggaatt agataagtcc tttggttttg ataaaaaact
     2461 ttatattaat tctgaaattt atactaatta cttaaatgaa aaatttactg atttaaaaat
     2521 agattttaat gaggcatatt ttgacctata ccttaaaaat tcagacttaa caatcggcaa
     2581 gcaaatgatt aactggggtt cagcctatga aattaatccc agttctatga caaatcctta
     2641 tgacctaaca gccttaaatc cgactgaaga aaagttggga gtaccagcta ttagaaatac
     2701 ttactatttc aacaacagaa cttctctaac tggggtagtt ataacagact ttgtgaaaag
     2761 tcctgttcca gccagcctta aggagaatat gataaaaggg ataaaagctg actttgcaga
     2821 acagctaaca aaacaaggag ttactaatcc caatctgata ttaaataaca atttagaagt
     2881 tgttgaatct gaacctgaga ttgatgaaat aggcgaatta gaatatgctg ttaacttaat
     2941 gaaaagaaat gtaagaggtt ttgacctctc agccagttac tttaatggtt atgaagatat
     3001 tccatccata acttctgatt tagataatgt taaacaagat ttaaaacaga ttgtgggaga
     3061 atatcaaaga aataattatt cagatactac aacagctttg gatattgact ataaaagaac
     3121 acagggaata ggattaaatg caattggatc aatctctagt gtgggagttt ggtcagaact
     3181 aaattatcaa attaatgaag atgatgagaa aacaatagat cttatattag gtggagatta
     3241 tacctttgat aataagcttt acactgtagc acaatttatt catagaggat atagtgatca
     3301 tcaaattccc agagaaagca tgaactacct cctcttacat ggagataagc catttaagca
     3361 gattcatacc attaaggcaa atcttatata tgacctggga ttaggaaatt acatgttcaa
     3421 tccagaaatg acattttcac tcagtaataa tattaattta gatttaggaa cagtgattac
     3481 tggtgaaaaa gaaggtactg atggatacgc cctctttaac atgatgagtg gagaaagaac
     3541 ttattttgag ctaacttata atttctaaat tatgaaaata tcgagcagga gagatgttga
     3601 tttattctct cctgtatttt tattttattt acctctttta aaatgataca ataaaaaaca
     3661 gataataatt cgtaatataa aatatataaa ggtgggagaa attaatggat agacccaact
     3721 gggatcaata tttcatggaa atggcggaat tagcttctaa gagatcaacc tgtttaagaa
     3781 gaaaagtggg agcggtattg gtgaaagata gaagaatact ggctacaggc tataatggag
     3841 ctcccaagaa attaccccac tgcgaagaaa cgggctgttt aagagagcaa aaaaatgtac
     3901 caagtggaca gcgccatgaa atatgtaggg gtattcatgc tgaacagaat ctaatcgctc
     3961 aatcggctgt tcatggggtt aagacagagg gggcgactgt atattgcact aatcaaccct
     4021 gtagtatatg tactaaactt ttaatcaatg ccggtattaa aaagatatat tataagaacc
     4081 catataatga tgagtttact aaaaagctac ttgatcagtc tgaaatagaa tataaggtta
     4141 taaaataaat tcaaaattgt aatatacttt cgtgcagcca tcttattttt atgttatcat
     4201 aacagttgga atcaaatcgg aataataaaa agatattaat aaggggttga ataattgatg
     4261 aacgtaaaag ttgacttaat ggaaaatttg aagtttaatg ctcaaggtga atcaggtcat
     4321 gatatgccga tggatgcttc ggaaagtgca ggtggagatg acagtgctgc tagaccgatg
     4381 gagatgctgt tagcaggtct cggaggctgt actagcattg atgtagtact tatcttgaat
     4441 aagatgaaag cggaaattga agattttaat gttgatatag aagctgagag ggaagatgaa
     4501 catccaaaaa ggtttaaaaa gattcattta aattattata ttaaaggtaa gaacttagat
     4561 aagaaaaaag ttgaaaaggc aataaattta tctgaaaata aatattgttc cgcttctgct
     4621 tcattaaatg cagaaattac ttcgtcatat gaaattgaga atattgaata ataatttttc
     4681 ataatcgcgg atatctattt gtggttttct aaaaataatt aacacataaa attaaataat
     4741 aattaataaa tgggagacag gccggttttg ggtctgtctt tttttaaatt aattttaaaa
     4801 aatttcttgt ataggaagga tattattaaa tattattgaa tatatataat cagaattaaa
     4861 taaaatttaa tatacatact tttaatatta aatactgttt aataaaaata aggaggaaaa
     4921 aaatgattga tttagagatt aatgaagacc aacttaaaag aacagttcaa agggcgagag
     4981 agaaaaatat tatcatgccc acctttgaac agatgaaaaa tccagaatta ataccggatg
     5041 aaatcaagga aaaactaaag gatattggat tatgggatat caattcttat aacctatttc
     5101 gtatcacctg gaaaaacgag ccgaaaaaag aaggcggact atacgatggt gttaactatt
     5161 tagaactacc ttctaaacta acgggagtcg atgccagaat aatcggagtc gtaggaaaat
     5221 ggtttccgac cggtgcccat aaggtaggag ctacttatgg atgtctggca cccagattaa
     5281 ttaccggtca gtttgatcct acttatcata aggcaatttg gccttcgacc ggcaattact
     5341 gccgcggggg agcctataac tcagaactat tagcctgtga ttcgatagct atcttaccgg
     5401 aagagatgag ccaggaaaga tttgaatggt tatccaatgt agccggtgag gtaattgcca
     5461 cacccggcag tgaaagtaat gtaaaagaga tctttgataa gacctgggaa cttaaggcag
     5521 aacgagataa tgtggtagcc tttaaccagt ttgaagagtt tggcaaccac ctctggcatt
     5581 atgaagtaac cggtagtgcc ctggaagaag tattagaaaa agagatgggt gaagatgaaa
     5641 attaccgggg tgtaacctta acctcgggtt ccgccggtac tttaggctgc ggcgattatc
     5701 taaaagagaa gtatccaacc agtaagattg ctgtcgggga agccctacag tgtcctactc
     5761 ttttaaacaa tggttttggt gctcatagaa ttgagggtat tggagataag catgtaccct
     5821 ggatccataa tgtgaaaaat actgatatgg taattgcggt tgatgatgaa gatacgatga
     5881 gccttgtcag gttatttaat gagccggccg gtcatcagta cttaagagaa gaattaggtt
     5941 tagagcagga ctttattgat aaattaaagt taatggggat ttcctgtata gccaatatga
     6001 ttatggctat taaatttgcc aagtattatg aattgggaga agatgatata gtactgaccg
     6061 tatttactga ttccatggaa ctttatcaat ccaggttaaa agagatggaa gaggagtttg
     6121 gtgaatatac caagaccgat gcagcagtag attataatgc tcacttgagc cgattatcaa
     6181 cagattacct agaagagtta acttatgaaa gcagaaaaag aatccataac ttgaaatact
     6241 atacctggat agaacagcag ggtagagaat tagaagaatt aaatgcacag tggtatgact
     6301 atgaaaacta ctggggcgag atccataaca tgactcctaa gatcgatgaa ctgattaaag
     6361 agtttaatga aagagtcgga ttattagaag aactttaaat tatctctaag aaggtgtaaa
     6421 gatgaaatat gtaaaaaatt taaaatgtac taactgtggt gaaacttatg aggcagtacc
     6481 agataggtat ttatgtgata aatgcggcga taaaggcatc ttagatgtac agtataaata
     6541 tgaagagata aaaaaagatt ggaacaagga aaagttagct aataatagtg agagttcaat
     6601 ttggaggtat ttgccgcttt taccgataga ggaagatacg aaaagaccta acttgagaat
     6661 aggtaatact cctatctata actcaaaaga gatgggtaaa aaactgggtc taaataatct
     6721 cattattaaa gatgatggat taaatccgac tggatcatta aaagataggg cttcagccat
     6781 ggcagctgta aaggccagag aagcaggtgc taaaactgtg gcctgttctt ctacaggtaa
     6841 tgcagcgtct tctttagcag gtaatattgc ctctatggaa gatgaaatga atgccattat
     6901 ctttgtacca gagagagctc cttccggcaa agttgcccaa cttttagtct atggagctga
     6961 agttgtatca gtacaggatt cctatgaggc ggcctattac ctttctgatg aagccattaa
     7021 aaaatggggc tggtataaca gaaatgcagc cattaatccc tatttagtag aagggaaaaa
     7081 gacctcagta ttagagatgg ccgaacagct taactgggag atgccggact gggtcgtatt
     7141 ttctgtaggt gatggctgta caattgccgg tgcctataaa ggactttatg atttgaaaca
     7201 gattggtttt atcgatagaa ttcctagatt attgggagtt caagctgccg gttgtgctcc
     7261 gatcactaaa gcttttagaa ccggtaaaga tcttgtagta acagatgaaa acacgattgc
     7321 agacagtatt gcggtgggta gtcccagaaa ttacaggaaa gccttaaatg ctgtaaagaa
     7381 ctcaaagggt gaatatatta atgtatctga tgaagagata cttaatatga ttaaattatt
     7441 gggtagtacc accgggatct ttggtgaacc agccggagta gcaggattgg ccgggattaa
     7501 aaatgctgtc aaagaaggaa taatcggtaa agaagagtca gtagccgtag tcattacagg
     7561 taacggttta aaagacatag ataatgcccg taaagcagct ggtgaattga ttgaaataga
     7621 agcagatata gataaattgg cagaaatatt tgataaaaag ggggtaatct aatggttgaa
     7681 gccaatatga agatgggtga aaagatccgt aatataagaa agtcccagag ccttagccta
     7741 tcggagttag cagataaaat tggtaaaacc tccagttatt taagtcaggt agagagaggt
     7801 ctggctgaac cctcaattac tgctttaaga gaaatatcta aagcgcttaa tatacctata
     7861 ttttactttt taatcgaaga tcaaaagcat aatgctgtag ttagaaagga agatagaaag
     7921 gtacttagat ttcctggttc acatttaaca tttgaactga tttcacctga tttggaacac
     7981 gatatcgaga tgatcgaagc gaagctagaa ccgggtgcca gtaccctgga taaaccatta
     8041 tctcataatg gggaagaagt aagtattgta atccagggga agatggaaat cacgattggt
     8101 agtgagagat atgagttaga tgaaggggac agtatttatt ataatgcaaa tttacctcat
     8161 aaaataacca gtattggtaa agaagagcta atatttattt cagcaataac accacctaac
     8221 ttttaatcaa caaatatcaa caatggaggt taaacaaata tgaaagagtt ttcagaaatt
     8281 ttaaaacagg ctgaaaaata tcgtcctgaa atttcgaagt tcttaaggga tatgattgca
     8341 attccgagtg aaagctgtca ggaaaaagaa gttatcttaa gaatcaagga agagatggaa
     8401 aaagttggtt ttgacaaagt tgaaattgac gatatgggta atgtgctggg ctacatcggt
     8461 catggtgatc atttaattgc gatggatgcc catatagata ccgtcggctt aggagataaa
     8521 gaactttggg attttgatcc cttagaaggt tatgaagata aggaagtaat tggaggtaga
     8581 ggtgccagtg accaggaagg cggtatggct tctatggttt atgctggtaa gattattaaa
     8641 gacctagacc tagaagatga ttatacctta gttgttaccg gtacagtcca ggaagaggac
     8701 tgtgacggcc tctgctggca ttatatgatc gaagaagaaa atatcagacc tgaatttgtt
     8761 gttattactg aaccttcttc ctgtaatatt tataggggtc accgcggtag aatggaaatt
     8821 aaggtaacta cccatggtgt tagctgtcac ggctcagcac ccagccgcgg agataatgcg
     8881 atctataaga tggcacctat cattaatgaa ctgaaggctt taaatgagaa tcttaaatat
     8941 gatgaatttt taggtaaagg gaccttaact gtatctgaaa tattcttcag ctcaccttct
     9001 agatgtgctg tagcagacgg ctgttctatt tctatcgata gaagactgac agccggtgag
     9061 agttgggagt ttgcccttca acagatacga aatcttcctt ccgttaagga agctaatgca
     9121 gacgtagaga tgtatgaata tgaaagacct tcttataccg gtcttagtta tcccattgaa
     9181 tcgtactttc ctacctgggt tattgaagaa gatcacccgg tctgcagtag tctggtagat
     9241 tcttatcaaa aattatttga tgatgaaccc ttagttgaca agtggacctt ttccaccaac
     9301 ggggtttcaa taatgggaag acatggaatt ccctgtatag gttttggacc tggacatgaa
     9361 gatcaagcac atgctcctaa tgaaaaaacc tggaaagatg aactagtaaa agcagccgcc
     9421 ttttatgcat tgattccaac agtttatgta aatgactatg ctgacaaaat gcctgaagta
     9481 accaaaaata ttgttgaata atttcaagga ggaataaaaa agatgaaaac tgagtttaga
     9541 ggtaaggact ttataacttt acaggactgg tcaaaagagg aactagatac cttattagaa
     9601 gtttcttttg atcttaagaa aaaatttgcg atgggtcttg atacacctta tttaaataac
     9661 aaaactgcta tgttaatgtt ctttgaacag tcaaccagga ctagaaactc aatggaagca
     9721 ggtttagctc aactgggagg gcatgcaaac tttttagata cttccaccat gcaggtatct
     9781 cacggtgaag ttgctaaaga tacagccgta atattaggta ggtttggtca tatgatagcc
     9841 tgtcgtaact gtgagtgggg tattggtaat gattatttaa caagtttagc caagtgggcg
     9901 cctgtacctg tattaagtct tcaggatgat ttataccatc caatgcaggg tattgcggac
     9961 ctaatgacca tcatggaaaa aagagaaaat actagaaatc taaaagtttc tatcatttgg
    10021 gcttatgcaa ccagtcataa gaaaccgatt tcagtacctg ttacacagtc acttcttttc
    10081 ccaagattcg ggatggatgt tactttagcc tatccagaag gatatgagct gcctgattgg
    10141 gtaattgaac agtccagagc taatgctgag aaacacggtg gttcctttaa gattactcat
    10201 gatatggaag tagcttatac agatgctgat gttgttattc ctaaaaactg gggcagctgg
    10261 gctacaagtc aaagtgatga tgtaatagat gaaaacttag aaagtaataa agaatggaaa
    10321 tgtaccgaag agatgatggc aatggcagat gaagacgtca tgtacatgca cgctttacct
    10381 gctgataggg gtaatgaagt tgaggattca gtaatagatg gtccacaatc catagtgtat
    10441 gatgaagcag aaaacagact tcacactgca aaagctgtta tgaccctaac aatgggcggt
    10501 aagtaattat ataatttaaa taacaactac ctcacccaaa tcatagaaaa attttgggtg
    10561 aggttcctat aaagctttgt tgattagtcc aaggcttaat tgactacgat accctagaat
    10621 aggtaaatac tttggagtgc tccacaagct ctccactcta cgttcaataa ttaaacacct
    10681 ttgcagggtt aaagaagtgt tgctgacata gaaaacctag ggatatcatt ggcgatgtag
    10741 actgacagct tggtggttta ccatcattgc ttattccctt acattaaagg atttgtaata
    10801 ttggtatagg ctaaaaactg atagggagac tggttgaaat ataaaactat tattgtaaac
    10861 gggaaatagg gatagagcag aaaggtaaga aagtgagaaa gttgctaaat tatgtatatg
    10921 tcatcgctaa agtcgacagg cccctgataa acaccaaaaa attcgggata ataaggcctt
    10981 ttcttgatga agataaggct gtatctttaa agaccaagcc ttttactata cagtttagct
    11041 atgagaccgg ccaccatata aaggatatta cggtgggggt tgatgcaggc tatcagaata
    11101 tcgggtattt gctgtaacca caaaagccga gtttttagtg ccgagattga gtttttaaag
    11161 aagataacaa agcttttaga ggaaataagg aaaaccgcag taaacgtagg agtgggttca
    11221 gacatagaga aaaagggttc aataatcgaa aagtatccaa gtctgaaggt tggcttgcat
    11281 tatcggttaa gcacaaggct tttttattta gaggtcaaga ccgtcgcact gatcttttac
    11341 taaatcaaaa atttggaagg ggttttctga gacattatta gtttaaaaag tttaaaagaa
    11401 tatataaaaa gcataatttt taaaattgtc aaaattgtaa taaaattaat atttttattt
    11461 aatctgctgg aatttagaaa cacagtagtt ttctctgttt tttcttcaat ttcttaaatt
    11521 tttaaaaata ttaaaaataa aaagggagaa ttaatatgag tgaaaatgta aataaaaggg
    11581 aagaaactga agcttattat aaattagaag atagaccacc tacgggtgag acgatcttat
    11641 taggtttcca gcatatgtta gccatgtttg taggtataat tacaccacct ttaattattg
    11701 caggagcggc cggcttaaat gcagccgagt cagctttttt cgttaatatg gccctgatca
    11761 tttcaggagt tgcttcttac atacagtgtc ataaatttgg accggttgga tctggtttac
    11821 tgggagtgca tggaactagc tttacctttg ttccgatggc tatctcagca gttaattcgg
    11881 gcggtttagc acttgtctta ggaatggctt tagcagcttc tcccgtggaa atgataatca
    11941 gtaggtttat taaacagact aaaaaatttt ttcctcctgt ggttacaggt gttgttgtta
    12001 tgctaatcgg actgggactg atggaaaccg gaatgactaa ctttggcggg ggtgctggag
    12061 cagaagactt tggtagtgtg cagaatcttt tactcggttc tttcgtcttg ttaattattg
    12121 tatttgccaa tagatttggt aaaggtttgg taaaagttgg ggctgttgca atcgggctgg
    12181 tagtaggcta tctggtatca atacctttag ggatggttga tttccaacct gtggctgagg
    12241 ccggttggtt tacagtacct ataccattta aatttggtat gtcctttaga tggtcccact
    12301 atttaccctg gattctagcc tatgtcatta cttccattga atcgataggt gacctaacag
    12361 cgatttcgga agtttctggt gaaccggtcg agggagatct ttaccgggaa aggatcagtg
    12421 gaggtattct ggctgatgga attggttctg ccctagcagc cgtattcaat actatgccca
    12481 atacaacgtt tagtcaaaat gtcggagtta tccagatgac aaaagtgggc agtagggttg
    12541 taggctatgc tgtagctggt atccttgtta tattaggact tttacctaaa ataggtgctt
    12601 taatcagtgt aatgcccaat gcggtattgg ggggagctac tatcgcttta tttggtatgg
    12661 ttgctacctc tgggatgaag atagtggtaa gaggcggatt aaccgaccgc aagatattta
    12721 tcctttcggt cgcactcagt atcggtttag gcgtaacctt taggccggat attgttgcaa
    12781 aactgcctga tattctatca actattttat catccggcat tacaatagga gcagtaagcg
    12841 ctatcctttt gaacttacta ttacctagaa aagaagaacc agttgaatgc taattaatat
    12901 tggttttaat taaaaatctt atatattctg tgatataata agtttaagat atatgactaa
    12961 acttatgaat taactaatat ttaatagtta aaaattaaaa caggaaataa cgaggttctg
    13021 tttaagatta aaaagtggag gtgttttaat ggagtttaaa catgtaaaca aatcggagaa
    13081 aagagtagat gtttacgaga aagtaaccgg taaagcaaag tatggagctg acatgagatt
    13141 cccagacatg ctttacggta aagttttacg tactgaatat ccctatgcgg aaataaaaaa
    13201 catagatact agtaaagctg aagagatgcc gggtgtagta gcagttatca cggcagctga
    13261 cattcccaac aatgaatttg gggtaattat tgaaaaccag caggtattag caaaagagaa
    13321 agcattatat atcggagacg ggattgcagt agtagcagcc gagacagcca aacaggctgc
    13381 taaagcggca gaaaaaatcg aagtagaata tgaagagtta gaaggtattt atgacccctt
    13441 gaagtcaagg gaagaagatg cccaattagt acatgaagaa ttagaagata atcaggttat
    13501 ccatcaccct ttaagaaaag gtgatattaa agaaggtttc gaaaaatcag atgtaattat
    13561 agagagggag tatgataccc cctttattga acagtcatat atcgaaccgg aagctgtaat
    13621 tgcagttccc tatcaggaaa attcaactgt aaccgtttat ggttcagtcc aaaacccttt
    13681 tgctactcag aatgcagtag ctacagttct aaaagaagat ttaaataaag tgaggattgt
    13741 gcaaaaccac atgggtggtt cctttggcgg caaggatgag gtagtttcat ccatggctgc
    13801 tcgtgcttca gttcttgctt taaagacaga aagaccaata aagcttgtca atactagaga
    13861 tgaatcaatt aaagaaagct ataaaagaca tccctatcac atgaagtaca agataggtgc
    13921 aactaaagat ggtaagattc aggccatgga aattgaagct gttgcagaca gcggtgctta
    13981 tgcctgtcag acccccttcg ttacctggag atctgttgta caggcgacag ggccttatga
    14041 agtggataat gttaaaacag atacgtacgg atactataca aataatgtgt atacaggggc
    14101 catgcggggt tatggttcac cacaggtaat atttgcgcag gaatccttaa tggatgaact
    14161 ggcagaagaa ttagagatgg acccggttga attaaggatg aaaaatatct atcataataa
    14221 ctcatttact gccagtggac aaaaacttga taaccacgaa gtgagcttag aagaagtact
    14281 cactaaagca gtagagaaaa gtaactttat tgaaaaatat aaagagtata gtaaagaaca
    14341 accgggagat aagaaaaaag gtatcggact atcgatcagt ttcaggggct gcagcctcgg
    14401 tgcggaagcg atcgatgcgg ccggtataat tttatcacta aaaaaagacg gcagtgtcag
    14461 cctttattcc ggactggctg aaaacggtca gggccttaag acagtttact gtcagatggc
    14521 ggcggaagcc ttaggcttag atactgaaaa gataaacttc atgcaggttg ataccttaat
    14581 ttccccggac agtggttcta ctgttgcttc acgtgcaacc ttaatcggtg gaaatgctgt
    14641 taaaaaagca gctgataact tgatagataa gatcaaagaa tatatctcag ataaatatga
    14701 tcaggccaag gaagatatat atcttaaaga tggttctatt tacggtgaaa aagatgtaga
    14761 aatcatgaca gtcaatgaga tggcagctaa agctgctatg gaaggagttt atctaaccac
    14821 atacggctgg tatgtatctc ccgatatttc ctgggatgaa gagaccggtc acggaaggcc
    14881 ttatttcacc tatgtctacg gctgtcaggt ggctgaagtt gaagtagata cggccaccgg
    14941 tgaaatcgat gtccttaata tgacagctgc ccatgatgta ggtcgggcaa ttaaccccgc
    15001 caatgtaaag ggacagatct acgggggagt atctatgggt ctaggttatg gggtcatgga
    15061 agaaatcgaa accgatgaag gatatattga aaatactaat tttgatgaat atatgattcc
    15121 aaccgttaag gatatgccgg atatagatcc gattattgta gaaaatcccg atccaaacgg
    15181 accttttggt gctaaatcga ttggggaacc cactttagaa ttaggagcag cagccatagc
    15241 caatgcagta gcaaatgcaa ccaaaaagag gatcagatca ttacctctta acctcgaaag
    15301 aattatcgta ggtaaggact tacacaaagg ggttgatcac aaatgataga atttgaagct
    15361 ttaaaagcca atagtttaga agaagcctta gatatcctgg ctgaaaagaa aaaggaagta
    15421 agaatggtag ccggtgggac tgatatgtta gttcagattc accacgaaag tgatgattta
    15481 aaaggtatgg aatacctctt agatatcagt tcgataccgg gcttagatct aatagaagat
    15541 aacaatgaat acctgaaaat aggtgcttta tgtacccaca gtgaaataat ggactctgtc
    15601 ttagtaaatg agaagttccc tattctaaag aaggcagcgg aagtcatcgg ttcaacccag
    15661 attagaaata ggggtacagt gggcggcaat atcaataatg cttccccagc agccgattta
    15721 gttactgccc tgattgcctt agatgcctct gtcgtgcttc attccaaaca cggtaagcgc
    15781 gagctttccc taagtgaatt tatcaccggt ccttatatga ccgatcggta tgaagacgaa
    15841 atactaacgg aagtcaaggt cccctatatc cagggcgaat actttgattg ttatcagaag
    15901 gttaaaaggc gtcaggcggt agcgattgcc agaatcaact tagctgtcat aaccaagttg
    15961 aaggataata agtttgatga tataagaatt gcctttggtt cagctacccc gacagctatg
    16021 agatttagag agatcgaagc agaattagaa ggtagtgacc ttaataagat agattatgat
    16081 aagttagcgg atgaaacagg acaggccatg gtaaatatca cgggcgagag atggtctacc
    16141 ccctataaga ggcctgttgt gggtaatcta ttaaagagag ccctccagtc aatagttaag
    16201 gaggtgcagt agtaatggaa gaagtaaatg ttactttaac ggttaatggt aaagaacatg
    16261 acttaaatgt ggcagctgat gaaagactgc tcgatacctt aagggacagg ttgcatctga
    16321 caggagtaaa agaaggctgc agtatcggag aatgtggagc ctgtacggta atcttagatg
    16381 gtaaagccgt aaactcctgt atggtctata cctttcagat agatggcagt gaggtcttaa
    16441 ctgtagaggg tttagaaaca aacggtaaac ttgatcccct ccaggaggcc tttattgacc
    16501 atcaggcagt ccagtgtggt ttctgtacac cagggatgct gatgtcagcg aaagccttac
    16561 taaaagaaaa tcccgatcca gatcgtgagg aaataaaaac ggcgatagaa ggtaacctct
    16621 gtagatgtac cggataccag cagatcgtgg atgcgattga aagtgttgtt aaaaaagacg
    16681 ggagtgaata accatgtcta aaatacttat caaagatatt aaagaattaa ttacgatgga
    16741 tgatgagagg aaacgatata aggatattga cttattgatt gaagatgagc aaattaaagc
    16801 aatagatgag aatattgata tctcagccgc tgaagttgat gaaattatag aagccgggaa
    16861 ctattttgcc tttcccggct taataaatac ccaccaccac ttctaccaga ccttaacccg
    16921 taatattcca gccgctcaag gggttgaact tttcaactgg cttaaattcc tatacccgat
    16981 ttggtcaaaa ctaaccccgg aagcagtcta ttatagtact ttacttgctt cagctgaact
    17041 tttaaaaacg ggttgtacaa ccagtgttga ccatttttat gtctttcccg acagccaaga
    17101 cgataactta atggattatc agtttaaagc agctaaagag atcggaatca gatttcacgg
    17161 cagtcggggt agtatgtctt taagtgaaga agatggcggc ctaccgcccg cctctgttgt
    17221 ccagagtgaa gatgagatct taaatgattc acagcgggtt attgataagt accatgatga
    17281 gagtgagtat ggtatgcaga gggttgtatt agctccctgt tcaccattct cggtttcaaa
    17341 agacttaatg attgaatcga taaaactggc caggcaaaat aatgtattaa gccataccca
    17401 tttggcggaa accctggatg aaaaccagta ctgtgttgat acctttggta aaaggcctct
    17461 aaaattaatg gaagaatggg actggattgg aaaagatgtc tggtttgccc atggggttca
    17521 ttttaacgaa tttgaaatcg ccaaattggc agaaacaggc acgggagttt ctcactgtcc
    17581 tgcttcgaac atgaagcttt cttctggggc agccaagata ccttatatgc ttaaaaaaca
    17641 ggtgccggtc agtctggcag tagatggaag tgccagtaat gatgcttcta acatgatttt
    17701 agaattaaaa cttgcttatc tactccataa attaatacac ggtatcaaga gtataacctc
    17761 tgaagatgtt ctatcgattg caactaatgg aggtagagat gtactgaacc agcctgaaat
    17821 tggcagcatt gaagaaggta aagcagctga tcttttcatg gttagaagtg atagattagg
    17881 atttgccggt ggacagagtg atccggttga agcgcttgta aataccggga ctactcaaga
    17941 agtagactat aatatagtta acggtaaagt tgtagttcgt aaaggagaat taacaactat
    18001 taaagaatat aatgtaataa agaagcataa tcaaattgct caagatcttt tgagtgaata
    18061 aggggggttg aaattgagac tatatggtaa cggtatctta atcaccttaa attcacaaga
    18121 ccctgtcatc aacgatggag cagtattaat cgataaagga attatcaaag attatggtaa
    18181 aacagataaa atgaaggtta gatatcagga agcggagttc gagaatgttg aaggtaaggt
    18241 tataatgcca gggatgatta actctcatat gcatttttac agtacctttg cccgcggcat
    18301 ggatttaaaa actgagattc caccccaaaa ctttacagaa atattagaaa agttatggtg
    18361 gcgtcttgat gaaaccttaa acagtgaaga tatatattac agtaccttat atgccttaat
    18421 tgaaagtatt aggagtggaa caactacgat ctttgatcac catgccagct atggctatat
    18481 tgaaaacagt ctcgatataa tatctgaggc ggtcatcaag tccaagctaa gaaccagtct
    18541 ctcctatgag atttcggacc ggcatggaga acaaaaaaca gaccaggcaa ttaaagaaaa
    18601 tgaaagattt ataaataggg ccaaaagcct taacagtgat tttataactc ccgccattgg
    18661 actgcatgct tcctttactt taagtgacga gaccttaaag gaagttgcag atctggcaga
    18721 gagtacaaat acaccaactc actttcatgc ggcggaaggg aaagttgatg aagaagatag
    18781 taaaaaacgc ggctataaga atctggccga tagatttgat aaactgaata tcttaagaga
    18841 agattcactg gccatccacg gcgtacatct gtcagaagaa gaatataaaa tacttgctga
    18901 taacggcgtc tatttaatcc ataacccgga atctaatatg ggtaatgcgg taggagccat
    18961 taatttaaga aaagccttag ataaaaatct cgtaatcggc ctgggaaccg atggatatac
    19021 taccgatatg tttgagagta tcaatgtggc agatcttctc cagaaacatg ataattctaa
    19081 tcctacttta gggggagaag atatcaaaaa gattgccttt gccaacaata gaaagatagc
    19141 aaagagctat ttcggtaaag aattaggagt aatcaagaag aatactcccg ccgatattat
    19201 aatagtagac tataaagcac ctaccccgat aaacgatgaa aactatttct accatatttt
    19261 attcggattt aacggtggtt tggtcgatac aacgatagtt aatggacacg ttttgatgga
    19321 aaaccaggag gtgaaagttg tagattatga aaaaacaatc gaaagatgta aaaaacaggc
    19381 aaaagacttc tggagaagat tctagtagtt actttcggcc taaatcaaaa gaaggtctat
    19441 tgcaaatttt aagagattat gaagaggatt tcaccataat tgcaggcggt actgaccttt
    19501 tagttgaaca ttaccaaaac ttatataaga ttgatagatg gttggactta aactgggtta
    19561 gtgagttcaa agaaatcaaa atctatgatg acaaagtaga gataggttcc atggtaaccc
    19621 atgaaagact gtataaatta gataaaataa aggaactcta tcctgtaatt caacaggcag
    19681 cagccgatgt cggttctccc cagattagaa gcaggggaac tatcggcggt aatattgtaa
    19741 catcctcacc ggccggtgac ttacttcccc cactcttagt ttatgatgcc aagttaaaac
    19801 tgatctcaaa agagggagaa agagttgtcg atgccaatga atttttcaca ggtcctaaac
    19861 gaaatgtaat ggaaaacggt gagatagtag aaaaaattat cttaccttta agagatgaaa
    19921 agtacaaagg agcctgggta aaagtaggta aaagaaaggc actcgtaatt tcttctataa
    19981 gtttagcttt aaatctagta tttgatgaag atgaaaagat aaaagaaagc agaatttgta
    20041 tcggatcagc agcaccggtt cccttaagaa tagagtcagc tgaagaacaa ttacttgata
    20101 agaaagcaag taaagttaag atagatgaag tagctcaaat cgtggctgat gagatttcac
    20161 cgatagatga tatcagggga actgctaaat accgcagaca ggtcgctttt aatataacta
    20221 aaagtgccct aagtgaggtg attaaccaat gaagataacc ttaacagtaa acggcaaaaa
    20281 aagagaagta gaagttaaac cacatacccg tcttttggat gtattgagag aagacctcgg
    20341 actaactgga accaaagaag gttgtgggaa gggtgaatgt ggggcctgta cagtcattat
    20401 ggataataaa gttgtagctt cctgtttggt tttagctccc caggcagatg ggtccgaaat
    20461 tcagactgtc gaaggattgg ctggaaaaga taaacttcat cccattcagc agtccttcat
    20521 tgaaaccggg gctgtccagt gcggattttg catacccggt atgattatgt cgagcaagaa
    20581 gttacttgat gaaaagataa acccgagtga agaagagatc aaaagaggct tatccggaaa
    20641 catctgtaga tgtaccggtt atgtaaagat catagatgct gtgaaagaag catcccacaa
    20701 gatgcaaagt gggggtgaag agaatgtcta aaagatatgt tggagaggac attttaaaag
    20761 tagatgctag agataaagtt acaggtcagg cccattatcc ctctgatcta aagttcgatg
    20821 actatttaca tgtcaagatc aaaagggcaa cacatcccca tgcgatttta aaggatttag
    20881 atattagtaa ggcgatgcaa gtggaggggg tagtcgatat aataactgaa gctgattatc
    20941 ctgatcttaa taaattcggt ttaataatca aagatcaacc ggttttaatt aaaataggtg
    21001 aaaagatgag atttatgggg gatgcccttg ctataatagt agccgaaaca gccgaggcgg
    21061 ctttaaaggc tagagaaaaa atagaagtag aagttgaaga actggaagtt atctcagatc
    21121 cccaccgggc aatcgaaaag gatgccccgg ctatccacga cgatttagat gatctctgca
    21181 cagaatatct aagtccagat gtaaatagaa agaatatctt ggttgatcat catttggaaa
    21241 aaggtgatgt agataaaggt tttgaacagg cagattttat agttaaaaat gattaccaga
    21301 cccaacatgt tgatcagctg cccttgcaaa atgaggctgg ttatgcggtt tatgatgaga
    21361 aatccggtga aattaaaatc tggtccgctt ctcagtggct gcatgataca caggctgatg
    21421 tagctcaatc actcaatatg tcgaaagata agataatgat catcaatgca gcaattggag
    21481 gagcctttgg taaaaaagaa gatgtttcgg tccatattca ccttgcctta gctgcaatgc
    21541 gtgtaaagag accggttaag ttaaactaca ctagagaaga atcaatgatt tctcaatcga
    21601 agagacaccc gcttaccatt agagctaaaa caggtgtaac taatgacggc tatctaacag
    21661 cctggcaggt ctttgtaatt ggagatactg gtgcttatgc ctcaagtgga cctgctgttg
    21721 tacacaaggg aatgtaccac tgtaccggtc catataatgt agcaaatgtg cgcggcacat
    21781 cttatgcagt atatacgaat aatacttatg gtggtgctat gcgcggtttt ggaactaccc
    21841 agatggcatt tgcctatgaa tcacagatgg atattatagc tcataaatta aatatggatc
    21901 ccgctcaatt caggtatcaa aatgcctatg ataccggctc tactactccc aatggccaga
    21961 tgttaggcaa gagcgtaaat acaaaacgaa ccatagaaga agcaatcaat ctgtcgaaat
    22021 gggaggggga gattaacaaa tgagaaaaaa aggtagagga atggcaacca taatgtttgg
    22081 ctttggatat ggggaaggtt tccccgatca ttccattgca gaagttgaga ttaccccgga
    22141 taaaagaata aaattgagaa cagctggggc cgatgtaggt cagggttttc tgaccaccat
    22201 gacccagatt gcagctgaag tattgaaaat agatacttcc tatattgaaa ttgtcggtca
    22261 ggatactcat agtgcaaaaa attctggttc tacttctgct accagacaga ctttcttttc
    22321 cggtaatgcg gtaagggaag cagctgaaga agtaatgggg aagatctacc atcatggtag
    22381 tctacatttt aacagtaacc atcccgagct aacagtaaaa gatggtgaat tatatgcctg
    22441 taatgaagaa aacaagataa cttactttga actggcggaa atggttgaag aaaagggaga
    22501 aaaactaagt gcagaagcat cctttttccc caagacttat gcacctgaca agaaaacggg
    22561 tcagaccgag aaaaactatg tggcctatac ctttatgacc caggttatgg atatagaagt
    22621 agatactgat actggaatta ttgaagtaat gaatgtttat acagctttag atgtaggaaa
    22681 ggcaattaat cctcagaatg tgcacggcca aatcgaaggc ggcaccgtgc agggtcttgg
    22741 gatggcctta atggaagagc aggttattga tgagggtatt accttaaatg cagatatgac
    22801 taattatctc gtacccagta ttaaggacat accagaattt gaaagtactt taattgaaaa
    22861 taaggattca gatggtccct ttggctctaa aggggtagga gaacctacct tgattgcagc
    22921 caatcctgcc gtggccaatg ccgtatacga cgcaataggg gttagaataa aaagtctacc
    22981 catcacaccg gaaaaaatct tagaggctct ggaggaacaa aaaagttaat gccaaataat
    23041 atactaatta ccggtgataa gtatgtcggt aaaagcacaa ttgtaaaaag aactttagat
    23101 aaattgaact taaaaccggg cggatttgtg gtaggtagaa ccggtaactt agatcagtgg
    23161 ttaagtttct acctcacaga tcccctggat tattactacc actggcaggg gaaaagttca
    23221 tctaagtata aaaagaaatg tcagattttt gcgaaaagag acgatcccag tcaagattgg
    23281 gagatccaac ccgatgtttt tgatgaaaag ggagttcagt ttttagaaac tggacgtaac
    23341 tttagagatg ttgtagtcat ggatgaactg gggagatttg agttagaggc agataaattt
    23401 caagccaaag tatttgaaat tttaaacagc aataaaccag tactggctgt aataaaagat
    23461 gagcataata aatttcttga taagataaga gatagagaag atatcagtat gtatagggta
    23521 aaagaaaaaa accatgaaac tgtatttgaa gatgtttata aagaattaag gagtattatt
    23581 ttagagatta acaaaaataa ggagtgatat taatggctga tttgcaagta gaatttgcgg
    23641 gaattaagtt agataatccc gttatgcccg ctgccggacc accagtcaaa gatgctaagg
    23701 cggctaaaag agccaaaaat ggtggggcag gagcaattgt tactaaaacg atctctacga
    23761 ctgctgctaa agtgccgaga ccaaatatgg caagaataaa aggcggtttt ttaaatactg
    23821 aactgtggtc agaattaact cctgaacact ggctggaaga agaatatcca aaaatcagag
    23881 aattagattt acctgtaatt gcaggtatcg gctatacaaa agatcaaata agagaattgg
    23941 ctaaaaaggt ggaaaagtat gccgatgctt tagagctttc tactcattac cttggtgaca
    24001 attccactcc catggaggaa agtatcaagg ctgccaaaga tgaggttgac atccccgttt
    24061 tcgtaaaatt aagtcccggt atcgatatac ctctatttgc taaagcggct gaagaagctg
    24121 gagccgatgg gattgtattg attaactcct ttggacctac cttagatatt gaccctcaga
    24181 ccggtaaacc attaatgggt agtgacaatg gttttggctt gctttcagga gaagctatct
    24241 ttcccttagc attaagggcc gtctatcaag cttccagtaa tgttgatatt ccagttattg
    24301 gggtcggtgg aattgacagc ggattaaggg cagccaaaat gtttatggcc ggagcatctg
    24361 ctgtacaggt ttgtactgaa gcaattgtaa atggtccgag tgtatataag aagataaatg
    24421 aagaactaaa taaattctta gatgaaaatg ggtatgaaag tgttaatgat atcaaaggct
    24481 taactgccca ggtagttaaa gaagaagcta attttaatac tatagttccg actgtaatcg
    24541 aagaaaattg tactacctgt ggtttatgta taaccagctg tgtatatgat gctatttcat
    24601 ataatgagag tagagactat attgttatag atcccgataa atgtgccggc tgtggtcttt
    24661 gtattacacg ctgtaacttt ggagccttaa agttagagta gattaaacat attgaacttt
    24721 aaaattaatg gaggggaggg attaagatgg ttaatgataa aatattaaag atggctaagg
    24781 gtgagattcc cgcggagttg gtattaaaac acggccgtat tgtcgatgtc tttaatgaga
    24841 ggttaattag agaagatatt gctattgcag atggtaaaat aattgggatt ggtgattatg
    24901 aaggagtcaa ggaaattgac tttgaggaaa atatcatagc tcccggtttt atagacgggc
    24961 accttcattt tgaaagtgcg atggttagag tagatgaatt tgccgcctgg attataccgc
    25021 gcggaaccac aaccgtattt gcagaccccc acgagatagc caatgtagca ggactggatg
    25081 gtattaagta ttttttagat cgcgggactg aactcccctg gaactttaat ctcatgcttc
    25141 cctcctgtgt acctgccact gctaatgaaa cgtccggggc agtattaact gccgataaat
    25201 tggaaaaact aaaagaggca gaaggaatct tcggcctggg cgaagtaatg gactttgtgg
    25261 gtgtaattaa aggtgataat aaaatttggg ataagataga tatgataggt gataaattta
    25321 tagacggcca tgcacccggt gtaagtggta aaaaccttaa tgcttattta ctgggtggaa
    25381 taatggctga ccatgaatgt actgaacccg aggaagcttt agaaaaggtt acaaaaggca
    25441 tgtatgtcat gattagagag ggctctgtaa cgagagacct ggtaagttta ttacctgcag
    25501 taaataacag taatctatcc cgattcttat ttgcaactga tgatagagat ccggaagact
    25561 tatatgaaaa cggccacatg aactttgtta ttaaaaaagc aatccaaaat ggaatttcac
    25621 ccttgcgcgc aattaaactg gcaactatta attctgccaa tgccttagac ttaaaaaatg
    25681 tgggagcaat cgcccctggt tataaagctg acctcgttat tttagataat ctgaaagatt
    25741 ttaatgtaga aaaagtcttt aaagatggac agcttgtggc agaaaatgaa agtttaattg
    25801 taaatttacc cgaaataaaa gaaggaaaat cggaaaaaat atttaaatca gtaaatattg
    25861 gtaaactaaa aaaagcagac ttcgatttac ctgattttaa agaatatagg gtaatgagtt
    25921 taataaaaga tcaaatagta acaggtaaag aaactattaa attagaaaat gaggttaaag
    25981 ttgatgattt ggtagccaat aacttagtta aaatagctgt agtggaaaga cataaaaaga
    26041 ctgggaatgt tggtttaggt ttacttaaag gttttggtct tcgcaaagga gctattgcta
    26101 tttccatcgg ccatgactct cacaatatta ttgtagtagg attaaattcg caggatatgt
    26161 atagagcagt taaagaaata gagagattac agggtggaat agctgttgtg gaaagcggtc
    26221 aattaattga ctgtttatct ttagaaatcg ccggtctaat gtcaaaagac tcaatacctg
    26281 aagtggcaaa aaaacttaga aaaatgaaag aaaaagtata ttcaatggga gtcaccagac
    26341 agagtccctt tatgacttta tctttcatgg ctttaccagt aattccttct ttaaaaataa
    26401 ctgatcaagg tttatttgat attgatgaaa ataaatttgt ttcgcttgga gtagacgatt
    26461 aatttaagag gaggtgttag aaagggcgaa taattgaaaa gtagtcttta atatttttta
    26521 attcaggact aatcaataat aaggagggaa tttgatgaac gaagtgaatg aaggaggctt
    26581 cctagacaga gtatttaagc ttaaggagaa taatactaat ccaaagactg aggtaattgc
    26641 tggttttact acctttttaa ccatggccta tataatattt gtaaatccat caatactcag
    26701 cgaagcaggg atgcctttta atggtgtatt tattgctaca attgctggta ttatattagg
    26761 tactcttgcg atggctttat taacaaacta tccatttgca ctggcttcag gtatgggcct
    26821 taatgcattt tttgcatatt ctgtagttat tggaatgggt gtttcctggc agacagcgtt
    26881 agggatcata ttcttagaag gtataatctt tattgtgtta agtgtgacgc cggtccggaa
    26941 gaagattgta aatgcaattc cgatgaacct aaagaccggt atcagtgcag gaattggttt
    27001 atttattgct tttatcggac tgcaaaatgc tggaatcgtt gttaatgatg aagctacttt
    27061 agttagcatg ggttccttat tatcaggacc agctctagtt gccctggtcg gattgatcgt
    27121 aacaggtatc ttacatgcta tgaaagttaa aggatcactc ctttggggta tcttaatagc
    27181 gacagccttt ggctggacta atggtgtaac acccgctttt aatggtattt ttgcgatgcc
    27241 caagatggct gattggaatc aggtgttatt ccagttagat attgcttcag ctttaaggtt
    27301 gagcatggta ggagtattat tatcattctt atttgttgac ctcttcgata cagccggaac
    27361 cttagttggg gtaagtaaac aggccggtta tattgatgaa gatggtaacc tgcctaaagc
    27421 tagtaatgcc ctacttgctg atgcgattgg tactactggt ggtgccctct ttggtaccag
    27481 tacagttact acttatatcg aatctgctgc tggtgtagct gaaggtggta gaacaggttt
    27541 aaccggtgta gttgctgcta tcttattctt cttagcactt ttctttagac cattaattgg
    27601 tcttgtaccc ggtgcagcta ctgctcccgc tttaataatt gtaggtacca tgatgatgac
    27661 taatgttact gaccttgact gggatgattt tacagaggta ttacctgcct ttattgctat
    27721 gattgcaatg cctttaacat attctatttc aaatggtatt gctttaggtt ttatcattta
    27781 tcctctagtt aaattattta caggtagagg taaagaagta aatgcattgg tttatgtctt
    27841 aggtgctgta tttattgcgt acttcttatg gatataatat aaacataaat aataggggag
    27901 gggcgaccct ccctttaaaa ataagaggat gatttagatg tatacagcta tcatattagc
    27961 cgccggtgaa gcggaccgga tgggtaagtt aaaacagtta ctcccctggc aagataagac
    28021 tatcttagaa acggttatag ataatgtttt agcaagtgag catatagatg atgaagtcag
    28081 ggtcgtcttg ggtgctgaag ctgagcgggt gaaaaataaa attgattatt atgatgatcc
    28141 gcgctttagg atcaaagaaa atcctgatta tagtcggggt atgagcagta gtttaagggt
    28201 gggcgtaaga aatttatcaa aaaacacaaa aagcctggtc atatttttgg gagaccaacc
    28261 attgattacc cctgatatat ttgataatat tataattgag tttgaaaaat catcttcaga
    28321 gataatgatg cctatctata atgaaaaacc gggtcatccg gtcataataa gtactaaata
    28381 tctagatgag atagaaaaat tagaaggtcc catgggttta aaacccttta tagaggagca
    28441 taaagacttg gttaactatt atcctataga agaagagaaa gtaactatag atcttgatta
    28501 ttatgatgat tatatttact acaaaaataa gtataattag ggttgatttt atgaaattaa
    28561 attataaact ttggttagaa gaagataagg aacgtctttt cggtgatgga ccagccgata
    28621 ttttaagact tgttgataga ctaggttctc tcagcaaggc cgctgcagag ataaatatgt
    28681 cctacagcca ggcctggcag ttaattgata aattagaaaa caaacttggc tttaaattgt
    28741 tagaaaaaca ggtcggcgga acagaaggcg ggggatctac tttaaccaaa aaaggtagac
    28801 tcctaatgga taccttctta agtttccgga ctgaagcgag gcgtgaaatg tctaaattag
    28861 aaaccaaata ttttacaaaa gaattcaaag ataaattaaa agaataagct actatttttt
    28921 taaaaccgtt atatgtggtc acaaataacg aaaagaagga ggaaataatc ttgaaaaaat
    28981 ttactagcaa aataactata attttaacac taatcttagt agtggggtta atggcatctt
    29041 catcagtcct ggccagtaaa atggttactg acatggcagg gagagaagtt gaagttcccg
    29101 atcaagtaaa tagggtggtg accacctatc gttcagcaac tcagtttgtt ttttgtttgg
    29161 gagtacagga taggttggtt gcttcagatt taagctttga aaaaatttca ttatttcaaa
    29221 aactatatga aggtgaaaag ctaccaaatg taggttcaaa aagacacggt ttgaatttag
    29281 agcagattat tgaaactgac ccggatctag taatcctttt cccttataat gatgggccag
    29341 aagttgcaga gaaattaaaa gaatttaata tagcatcgat tattatcgta cctgaaagtt
    29401 atgaacagat aaaagaaacc accagaattt taggtaaggc cttaggtgtt gaagagaggg
    29461 cccaacaggt tatcaaccag tatgacaaaa tactggatat agttgataaa aaagatagta
    29521 tcgaagagaa aaagaaggta tactttgcta attcagatct atttgataca gtcggtaaga
    29581 acatgctgca gaccagtatg atagaattag ccggtggtct taatccagct aaagaggtta
    29641 aatccggatt tattaaaact tctttagaag agttaattaa atggaatccc gaggtaatag
    29701 ttgtatctca atattacaat gggactgtag aagatttgtt ggcaagaccg gagctgcagc
    29761 agttaaaggc ggttaaaaat gagaatgtct acagattccc ttctaaagta gaaccctggg
    29821 atttcccgag tccatcttct tacttaggaa tagtatggtt agcagaaaaa ctttaccctg
    29881 aaaaatatag tgatcttaag ttagaagcag ttgtgaatga atattataat actctatatg
    29941 aaaaaagtta taaagagtta ggtggaagta attcataaga ttagaaggtg taaatatgtt
    30001 agaagttaaa aatctaggtt tttcctataa aaaagttaaa acactcaata atataaactt
    30061 cagggtcaga aggcgggagt tctcctgcct tctgggagct aatggttctg gtaagactac
    30121 tatcttaaaa tgtctaaatg gtgttttaga taaggatgaa ggcaaggtaa agatcgacca
    30181 gaccgacctt gataacctaa accagaggca gattgcccag tatattagca tggtacccca
    30241 agaacattcg gtcgtatttt cttatcgggt tttagatgtg gtgacaatgg gggcaactcc
    30301 ttatctaggt tttggtaaaa tgcccggcca aaaacattat gacagggctt ttcaaatact
    30361 tgcaaaatta gatattgaac accttgctga cagacagtat aatcagttga gtggtggtga
    30421 aaaacaactt gtcttaatcg cccgggcctt aatgcaggat accggttatt tgatcatgga
    30481 tgagcctact tctcatctgg acttcaaaaa caagaacctg ttaatgagag agataaagaa
    30541 attaactcgg aatagtgaga caggtgtaat tatggcctta catgatccca atttggctat
    30601 gaaatactgt gaccgagtca taattgtcaa agagggagcc attatggccg atggtgatgc
    30661 aaatgagatt ttaacaggtc aaaatctcgg tgatgcttac gggattgata taaaaattaa
    30721 tgaaaaagca agaggaattg agatagccct tcccgaaata taatattaat gggaagggag
    30781 tttttttatt ttgagttata aaaaaagcat tatttctgga tttaaaaaat caatacctgt
    30841 aattttaggt tatctaccga ttggaatcag ctacggcatt ttagccttac agacagattt
    30901 atcaccatac ttgattgtgg ggatgtctgt gttcgttttt gcgggttcct cacagcttat
    30961 tgcagtcaat atgttaactg cccaggctgc tctgattccg gttatcatga ctaccttttt
    31021 ggttaatttg agacatatct tgatgagtgc ctccttaagt ttgcatttca aaaaaactcc
    31081 ccaaaagtta cttcctttta tcggttttgt catcacagat gaatcctttg ccctgggtaa
    31141 taccctatta aatgatcggg aagaaaacaa ggggatattt ttcctatcta tgggtctgag
    31201 tgcctattta ggctgggttt taagttcctt agccggggta ataatcggca ggtatatcct
    31261 aaattttgag atacctgtga tagactttgt tctgcccgct atgtttatta ttttacttat
    31321 catgcagatt aatgagaaga tggatatagt agtttctatc atggcggccg ttctttctat
    31381 aatcttttat tttattctgc cgggtaattg gaatatcatt gtggcaacag taaccgcggc
    31441 cgtagcaggg gtgatcatag aaaatgcaat ctaaatatat attgatgatt ttgggcatga
    31501 cactggtcac ttatataccc aggatgctgc ccgtaacttt gctttcaaag gtaaaaatac
    31561 ccgataccat ggtgaggatt ttaaagtttg tagggccggc tattttagca tcattattgg
    31621 cgccagccct atttattacc gagggttcag ttgatatttc cctacataac agctatcttt
    31681 tagcagcaat tcctactttt tttaccgctt attttacccg cagtatcttt accactgtct
    31741 ttttaggcat gttatattta tatattttca cattaattta agagaggtga gatgatggat
    31801 attaaaatcg atttatttag tgatactggt accagacctt ctgatgagat gagaaagttt
    31861 atggcagaag ctgaagtagg tgatgaacaa ctatttgaag accccagtgt aaataggtta
    31921 actgccatgg cgtccgaatt attgggaaaa gaggaagcaa tctatttacc atctggtcta
    31981 atggccaatc agatttcctt tgccgtccat tgtaatcccg gtgatgaggt aattatggac
    32041 caaagtgccc atcccattca ttatgaagga ggggcgaccg ctgtgataag cggtgcttcc
    32101 atccggggcc tagaagggga aaaaggtatt tttcagttag aacagtttga agatgcggtt
    32161 agaacaaaag atttccatga cccgcaaagc aaagtgctgt ctattgaaca aacctcaaat
    32221 ctgggcggag gcagaatttg gcccctggat ttaattaata acctgacaga aagggcccat
    32281 gaactcggtt tgaaaaccca catggatgga gcaagactat ttaatgcggt agctgcaacc
    32341 ggtatttcag ccgaagagta tgcggctaac tttgattcgg tctggtttga tttaagtaaa
    32401 ggcttagggg caccggtcgg gtctgtatta gccggttccc aggaatttat aaataaggcc
    32461 cgctactgga aacaaagatt gggtggtgcc atgcggcagg caggtattat tgcggccggt
    32521 ggaatttatg cgcttgagaa taacttagag agattaaaag atgataataa aaatgcaaag
    32581 cttttggcag ccagattggc agaactggat aaagttgaaa ttgatccgtc tactgtggat
    32641 acaaatatag ttatttttaa gataaaagga gaaagttctg ctgaatttgc ggataagctt
    32701 ttagaagctg gtctaagggt aagtgtacta catgataaat taagggctgt aactcatctt
    32761 gatgtagata gaaatgatat attagaagca gttgagattt ttaagaaaaa gctataaata
    32821 taatactaac atttatgtcg gtaactttaa tgaccctgtt aaaacaagac agaccacaga
    32881 atctttaatt tcatctttaa tttcagaggt aattattagt ggtgttaacc tgggaaatta
    32941 cgaactattt agagctgtcc aaaacgcaga tagacaaata tatattacaa ctacttatac
    33001 aaacaaacac tatatagcta gcacctgacc atcatttgac ttctgattaa ttcttggtat
    33061 tacaatggtt atcttaggga tcggtctctc agatttatta tataagatta tcataggagt
    33121 acaacttttt gcaccggaag cacccccagt gccagttata gatattccct tacttagtaa
    33181 tattcctatt attgcagcca tatttaatca ggatgttata gtttacttta cttatatagc
    33241 agtatttgtc gcttattata ttttctatca caccagagtg ggattagaaa caagggcaat
    33301 tggtgaagac cccaaggcag ctgatgttgt aagaattaat gttaccttaa aaagatacgt
    33361 tgctacaatt accggcggta tgttgatgag aatggcagga gcgtacttac cccttgtaat
    33421 taccgggact tataccacta atatatccaa tggtagaggg ttcatggcca tcgggattgc
    33481 tatatttgcc agctggaggc cggcgagagc ttacttaggg gcatttatct ttgctgccat
    33541 tgaagtgctt tctttccaac agcagttgga acaatcgatg ttcccctatc agttcttatt
    33601 aatgctgcct tttatttcag ttttgatcag catttggatt ttcagaaaaa gggtcgaatt
    33661 cccggcccca cttggtaaac cttacagtag ggaataagat gagaagatat caaagcagcc
    33721 cccaagggct gtttttttct tatcaattct gtataatttt tttagtttct attttaaatt
    33781 tttagattgc tttcccggca ataataccga ttagaacagc taaaattccc aatgtcaaat
    33841 tgccgaagat attaattccc gccattaaga aacttttttc ttctaataat accattgttt
    33901 cataactgaa agtagaaaat gtagtaaaag cacctaaaaa tcctgttgtt agaaggatct
    33961 ttaaattcga aaaaccagta actttttcta aaaagagtat cattaaaaag cctattaaaa
    34021 aggaacccac tgtattgaca actaaagtac cattgggaat taaactatcc cattttatct
    34081 ctatccattt tgagagcaga tatcgactgc tggctcccat gaatcctcca atacctatgt
    34141 acagtatttt taacatttga tcactcccca aaaaggttta taaaaatata aaaagcccct
    34201 gcttagccta aaggctaaac aggagccatt attcagttag actgacagtt aaaggtgagc
    34261 tccatcacct aattaatttt tctacttaat tatatatata gtttcaggta aagtcaacaa
    34321 atagtcttat ctttaataag aggaaaatag atattgttgt ttaaattata tattaggaga
    34381 agttaaatga agatgttttt tataagagag aaaggatgat ttgatatgga gagtatttta
    34441 aggtattcaa ccgaagtaga aaaagccata aaagaggata aaccaattgt ggccttggaa
    34501 tcaactatta tagcccatgg ttttccctat ccagataata ttgaattagc aattgaatta
    34561 gaagaacagg cgcgagaggc gggagtagta cctgctacta ttgcaattat agatggtaaa
    34621 ttaaaagtag gattaacaaa agaagaatta gaatttatag gtaagtctga tgacattaaa
    34681 aaagcgagtt taagagactt gcctgtcctg gtatcaaaaa agttaaacgg ggctacaaca
    34741 gtggcagcta caatgcagat agcttatctg gccggtattg atgtctttgt aaccggtgga
    34801 attgggggag tgcacagaaa agctgaagag acctttgata tttcagctga tctaagggct
    34861 ctttctgagt ttcctgtaac tgtggtctgt gctggagcaa aatccgtctt agatctaccc
    34921 aaaacaaaag aagtattaga atcaaaaggt gtaccaattt taggatataa gaccgagaag
    34981 tttcccgctt tttacttaag agacagcggc ctggaagttg attattcggt cagtaatggt
    35041 aaagaagtag ccgagatagt taaaaataat atgctgatag aaaatagaaa tggaatctta
    35101 gttacagtac ctattccaaa aaaagatgaa cttaataggt ctcaatttaa taaaataatc
    35161 gatgaactag ttcgggaagt agaagatgaa aatattaggg gaaaagacat taccccctat
    35221 cttttagcga ggataaagga agaaacagag ggtgaaagtg ttaaggcaaa tatatcacta
    35281 gttaaaaaca atttaagagc aggctgtgag atagcaaaat cagtaaagga tctgagataa
    35341 aatgatttta gttatcggag acattaacct tgatatcatt gtaaatttaa gagaggagat
    35401 aaattttgat accgataccc agtctgaaat ttacttcgcc ggtggtggat cgtctgcaaa
    35461 cttctcttac tggcttgatt atcttaagca gccggtccgg ctggtcgcca aaacgggtga
    35521 cgactttatt gtagattatt taaaagaaga atttaaggat accaatattg actttattaa
    35581 tctaaccagc caagaaaaac aaacaggccg ggtggtagta ttattaagca aggccggtga
    35641 aaggtcgatg attacagacc ggggagctaa tatagatatt aagccctctg atattaaaga
    35701 aaaattattc aaagatgttg agcagctgca tttaggaggt tacagctttt tcgggggagc
    35761 caatatggaa aaaaccgcct tgagagctat aaagatagca aaagataaaa atttactaat
    35821 ttcttttgat ccttcctcct attctggttt aaaggaatat gggatacaga agatattaaa
    35881 tcaaacagcc ggtattgatc tatgctttcc caatatagaa gaagggcaag tactgaccga
    35941 taaaaagaag ccgaaagaaa tcactcacta cttacttaat tattataaga atgtaattct
    36001 aaaagtaggt aaaaaaggct gtatggtagg caatcaagag gaggtttcct ttatacagca
    36061 aaaaaggaaa ttagcagaag gagatacgac cggagccgga gatgcttttt cagcagcttt
    36121 tattgccaaa tattctgaga caaaaaatct gaaagaagct ggtgactacg ctaatcaaat
    36181 aggttttgaa tgtgtaaata aaattggtgg aagaccatat aacagggagg caagataaga
    36241 tatggaaaaa agaaaactag gtaaaacagg acttgaagta agtttgattg gttttggtgg
    36301 gtttcattta ctcgaaattc cagccgaaaa agcgcgaagt ttattaaata attatattga
    36361 tatgggagga aattacatag aaactgccgc cgactatgga gatggagaat cggaaagaaa
    36421 gatcgggccg gtcattgaaa agagaagaga tgaagtagtg ttggccagca agaccggttc
    36481 tagaattaaa gaaggtgccc taagagatgt taaccgcacg ttaaataatc ttcacaccga
    36541 ccatcttgat atattattta tgcacgcagt aaaaagtttt gaggaattag aaacgatctt
    36601 ttcagacggt ggagcctatg aagcattttt agagttaaaa gaagcaggca aagtagataa
    36661 tttaggtata tcaatgcacg gccaacctga tgtattaata aaagccctta ataaagataa
    36721 atttgatgta gtgatggcca cctttaatta ctttgataaa ttcaactttc ctaaattaga
    36781 agatgaactt ttacctttgg cacaggaaaa agaggttggg attgttctaa tgaaagcatt
    36841 agctgacggt ttcttatgga ggtcagctga aactgctttt agatatgcgt tttcattacc
    36901 ggtttcaata gtagtgacgg gtattaatac tgaagatatg ttagaagaag atattggata
    36961 tgcaaatact tatgagccga tgagtgaaga agaaaaagag gaactttaca agaacgcacc
    37021 agagctgggt aattatgtct gccgccagtg tgatgaatgt tttgactgtc cggtcgatat
    37081 agatatcaaa gaaatattta aattagaagg atattatgac cgtcaaatga gagacggtaa
    37141 ggtcagaaac ccggctgatt ttgccctaag agacagatta agattttggt ttgaaggaga
    37201 agaaaaggca aggtcccttt atcaagaatt agatgttaag gcagatcagt gtaccgagtg
    37261 tgaaaaatgt cttgaatact gtccttatga cttaccgatc atggaaaaac tggatatcgc
    37321 ccattataag ttaagtggtg aggatgtatt gttttaattt taaataagta agcattgaac
    37381 cgggagctaa atttaactcc cggtcttatt tgtattaaaa ggatcatagt attatttttt
    37441 aatggattta aaaccatatt taggtggagg agagaaaaga gaagcaaaca cagcaagagt
    37501 aggtagtatt aaaattccaa tcatgatttc ttgatagcca ccaaaataat cataagctac
    37561 tccaaagggt aaaggaccca gagcagaacc aattaccatt gtagtcattg caaaacctct
    37621 tatacttccc agattttctc taccaaaata gttgggccaa actaccccgg tactgacagc
    37681 atcaaatgcc ataaatgtac catggaccaa gccatatatt attaatgcaa ttttactgtc
    37741 tgcttttaat aataatataa ttgctccgga taaaatgaga aagttaatgc ccttaataat
    37801 attaacttta agccggtcca acaaccagcc ggctataaaa gtaaagggaa gctttatcat
    37861 tgcggttata cttaagatta aagcagcaaa acccggtgaa taaccaatgt tatttacaat
    37921 tgaaaccatg tggaaggtta ggcctgtatt gactaatgca gatataaccc cacaataatt
    37981 cataaaccaa aatgacctag tccccataga ttgttttaag gtccagggat cttcacttat
    38041 atgaaccttt ggagttatat tatttgtata ttcttcttca ataccatcag gttttagtcc
    38101 aatattttcc ggcttatttc taataaaaaa ccaacctacg ggggccatta gaaagatgag
    38161 tagaaaagcc caaaacctcc aagtaaaatt caaactatat gtattaatta accagttatt
    38221 aaggggaggt aaaagactag aacttataac accgccaact cctaacagac ttagcgcgag
    38281 tcctcttttt tgtttaaacc agtggggaac tatagtctgt ggtaaaagag tcatggagcc
    38341 ttggcctaaa agtcgtagta aaaagaaacc aacaaatatc atgaaccgat taaatacaaa
    38401 actcatccat atacatacaa gaccaaataa aactgatatt acagtagtca tttttctatg
    38461 tcctaaccta tcgatatatc taccgataaa tgtaagaatc agaccagata ttaaagttgc
    38521 tattgaatag aagcttgaaa tcaatgagcg gctccagtta aatttttcaa tatagtggtt
    38581 tataaaaatg gaaatagagt aagtttgtcc aggccccgag aaaaacaaac ctaaggcggc
    38641 tataaataat ataagccagc cataataaaa aggtgtttct attggttttt gttccttaaa
    38701 gtttttgttt gtcataaata ttcatccttt ttacatgttt ttataaatta aatattctat
    38761 acttacttat atatctataa cattaatatt agttgatgtc aaatagaagt ccaatgatag
    38821 tgtcaagtta cttttggatt taagataaac tccctttaag atttatttaa aaggcatatg
    38881 cctttattgc ccgatatgtt ccgctaactt taccattttt taatgctggt agtacttctt
    38941 tggcctcagg atat
//
LOCUS       DATGCJ010000010         9352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_447550, whole genome shotgun sequence.
ACCESSION   DATGCJ010000010 DATGCJ010000000
VERSION     DATGCJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447550"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VKN64_00460"
     CDS             complement(<1..193)
                     /locus_tag="VKN64_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HMA58733.1"
                     /translation="MYNTSEELKNAVNASKKYIENEGLKVLVCAGTGCVANGSLEIFE
                     DLKEKVESLEMPVKVELFEE"
     gene            complement(186..665)
                     /gene="nuoE"
                     /locus_tag="VKN64_00465"
     CDS             complement(186..665)
                     /gene="nuoE"
                     /locus_tag="VKN64_00465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMA58734.1"
                     /translation="MDIDLNSIKPIVKKHAGEKGGLIPILNEVQRELGYLPSEAQEYI
                     SDELNIPLSKIYGVITFYTQFSTKPKGKYVIHACDGTACHVRDSQDIIDTIKDELSLQ
                     KENSTTEDGKFTLETVSCIGACGLAPVVTINEKVYGKLTPDKIREVIEEYKGSEDIV"
     gene            complement(702..1280)
                     /locus_tag="VKN64_00470"
     CDS             complement(702..1280)
                     /locus_tag="VKN64_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HMA58735.1"
                     /translation="MVKILVSGKSKASGKTTLVEKIINNLRGNIYVIKSSIHDKYDKF
                     ELIDDKKIILEKDTDTEIFSRAGADKVYYLKSNRANLKEGIEQEFNKIKGYDYLIVEG
                     NSIIDFVGFNFVYYLDKKGGDKKKSAEKCKKKADVIINSDNDYEIEFNQDVISCFKAH
                     LLGHSLNIPLKKVGDMTNEANIKVKNCQLGLF"
     gene            complement(1422..1652)
                     /locus_tag="VKN64_00475"
     CDS             complement(1422..1652)
                     /locus_tag="VKN64_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMA58736.1"
                     /translation="MRKFEVFGSGCPNCKKAESNIREVIDELDLEAEVIKVQDMNEMA
                     ERGVMLTPAVALDGEMKVTGKVPTKEEIKGWI"
     gene            complement(1675..2847)
                     /locus_tag="VKN64_00480"
     CDS             complement(1675..2847)
                     /locus_tag="VKN64_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HMA58737.1"
                     /translation="MSKVKKLSLYIAVFLAAFYVPINNPKFQQALLESFYMVKEYAQE
                     HVLFCLIPAFFIAGAIANFVSKNAVIKYFGSEAKKWVSYSVASVSGTVLAVCSCTVLP
                     LFAGIYKRGAGIGPATAFLYSGPAINVLAIILTARVLGWQLGLARAVGAIVFAVVIGV
                     LMAIIFRKEDKERLKGDFALGDDSKESRNSFQNLIYFVTLVLILIFAAWAKPSSETGF
                     WRFIFSIKWPVTITLLVFLGIQLKSWFTKNELKDWVDSTWDFAQQILPLLFAGVIVAG
                     FLMGRPGNEAGIIPASWISNFVGGNSLLANFTASILGAFMYFATLTEIPILQGLMGLG
                     MGKGPALALLLAGPALSLPNMLVIRSVMGTKKTLVFISLVVVMATFSGFIYGTFVI"
     gene            complement(2868..3179)
                     /locus_tag="VKN64_00485"
     CDS             complement(2868..3179)
                     /locus_tag="VKN64_00485"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMA58738.1"
                     /translation="MEEKLFEAREKIMKSLANKYRLKIVDSLGKKSKMCVSDLEEALD
                     LNQSSVSKHLNILKEAGVVKSKKEGLKVYYSLRTPCIINFFACVDNVIKDEINERLNL
                     F"
     gene            complement(3372..4088)
                     /locus_tag="VKN64_00490"
     CDS             complement(3372..4088)
                     /locus_tag="VKN64_00490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA58739.1"
                     /translation="MGLKWYEDLYEDYGKSYEEEPFVKGTEGEVDFIEKELDFNKDKN
                     ILDIGCGTGRHSLELAKRGYKVTGIDLSESMIEQAKRKREEAGLDVTFKIKNALNLDY
                     HKDFDLSIILCEGAFSLMEDDEKDYRILKNAYNALKDNGKLILTVLNVFKQIAEDNKN
                     LDLKTFREEFELEVADDNGKEKTIQAKQRYYTPSEIKWRLKNIGFKQIDIYGCELGNF
                     NRKRKVNKKDMEFLVIAKKE"
     gene            complement(4114..5136)
                     /gene="rsgA"
                     /locus_tag="VKN64_00495"
     CDS             complement(4114..5136)
                     /gene="rsgA"
                     /locus_tag="VKN64_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMA58740.1"
                     /translation="MNFNKLGLNRFEYNIEDKNKLARVSAQYRDLYRIITTEGEIMAT
                     LSGSSYFKSSKGNFPVVGDWVIFDQYNKESNGSIKEVLTRKSQLSRNQAGKKEAEQII
                     AANIDIIFIVTSLNQDFNPRRLERYMTIAWDSGAKPIIILNKSDLCDDREYYIDKINN
                     IAFGTEYIITSCVNNEGIEKIREILNPDKTAVLVGSSGVGKSSIINLLLEEKKQDIKD
                     IRADDARGRHTTTNRELFLVPDGGVIIDTPGMREIQLWVDENSLDNVFSEIEQLARDC
                     KFNDCTHTHEPGCEVKNAVKDGKLSEDRLNNYFKMKKEIEYQKLKEKYNAKKANKIKW
                     QKLMKN"
     gene            complement(5490..6863)
                     /locus_tag="VKN64_00500"
     CDS             complement(5490..6863)
                     /locus_tag="VKN64_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMA58741.1"
                     /translation="MKEIEKHSAKYKYIIEGPILKSLITLAWPIMLSQLMQTLYNLAD
                     TLWLGRVGPEAVAAISISFPIVFMMISIAAGLTIAGTALIAQHKGRGNVKRINNIIGQ
                     LFSFIVFLAIVISIVGSVLANDIIILMGAEPEIVPDAAGYLRTIFTGMPFMFIFFIFS
                     SILRGIGDTKTPSIMMFFSVTINIILDPLFIFGIGFFPELGVVGAALATIIARAIVAI
                     YALIIIEKGVDGFYLKLSNLKWDFSIIKKIIRIGVPSSVEQSMISLGQVLMTSLVAGF
                     GTMTLAAYGIVNRIISLPTILAFGVAAAATTMVGQNIGADKKERAEKTALVSIGIIFI
                     SMSILGIVLFFEPRFVIKIFNDAPAVVKYGSSYLRIVALTFGFLGAMYVGNGVFKGAG
                     KTVPPMVISSSSLWVFRLGIAYLLITIFSMSQVGLWWAVALSHLGGSLLGLIWLKISN
                     WSTAVID"
     gene            complement(6928..7701)
                     /locus_tag="VKN64_00505"
     CDS             complement(6928..7701)
                     /locus_tag="VKN64_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58742.1"
                     /translation="MDIKKKYMKIFRKNLYLNSKKSKKIIKQVEAEIDDILSRYDDEA
                     KAYNEEMEGAIPYALRLTRENADYIEDMGLVNAIKFSSKVPHVDIKSRMEIGNLPVFH
                     LTSGFNREKGDYDVAKGIIAIGSKARGVIAFGKLAYGLIAIGGLSIGLLSFGFLSAGV
                     FSVAGLAFAFLLAIGGISISAIASIGIVALSSLAASGRVAMAHFVQTGQKMSENMPAW
                     FAAMANNIGMVILVFEMIIIGIGLLIFYFEYISKRNYDD"
     gene            complement(7725..8825)
                     /locus_tag="VKN64_00510"
     CDS             complement(7725..8825)
                     /locus_tag="VKN64_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMA58743.1"
                     /translation="MSKVYFADIRARSEGTSKIKKIKKLFDEAGFGDLIEEEDFTAVK
                     IHFGEHGNDGYLNPVFARQVVDKIKEHKGKPFVADTNTLYSGMRHNSIDHHTVANLHG
                     FNFSVLDAPVIIADGLRSQNFLEVEIGAKHFNKVKIASDFVHADGMIVMSHFKGHEIA
                     GFGGSIKNIAMGCAPAAGKKDQHSVDIESDNDKCIACGTCAEVCPENAIVVEDFAVID
                     PKKCIGCGECITACPVDAIGMDWESEIRPFMERMTEYAYGAVKNKLDKVGYINFLLDI
                     TPECDCAPFSDAPLVRDIGILASKDPIALDKASYDLVNKQEGLRHSHLEKNYKPGEDK
                     FKGSRDHTEGTIQLTYGEELGLGETGYELIEI"
     gene            complement(8881..>9352)
                     /locus_tag="VKN64_00515"
     CDS             complement(8881..>9352)
                     /locus_tag="VKN64_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA58744.1"
                     /translation="ESNNPCSKCAHFKKGALVDYMKANGFEKLAFGHHYDDAVETFLM
                     SILYSGQVNTLQPKRFLSNNEVYIIRPLIYLREKRIRAAKNRIEYDDSFVKCPYDKNT
                     KREEIKTILDKIKFNKQIFHNIAAAMREDSIKEMWPVKMEQNILSDKIDMIWNK"
BASE COUNT         2889 a         2003 c         1203 g         3257 t
ORIGIN      
        1 gttcttcaaa taattctacc ttgaccggca tttctaaaga ttctactttc tcttttaaat
       61 cttcaaagat ttctaatgaa ccattcgcaa cacaacctgt acctgcacaa actaatactt
      121 ttaaaccttc attttctatg tattttttag aagcatttac tgcatttttt aattcctcac
      181 tagtattata caatatcttc actcccctta tattcctcaa ttacttctct tatcttatca
      241 ggagttaatt tgccataaac cttttcatta atagttacaa ctggagccaa accacatgct
      301 cctatacatg aaactgtttc tagagtaaac ttgccatcct cagtagtact gttttctttt
      361 tgtaaactaa gttcatcttt aatcgtatca attatgtcct gcgaatctct cacatgacag
      421 gcggttccat cacaggcatg tattacatat ttacccttag gtttagtaga aaattgggta
      481 taaaaggtga taaccccata tattttactc aagggaatat tcaattcatc acttatatat
      541 tcctgcgcct cagatggtaa gtaacctaac tctctctgca cttcatttaa aattggaatt
      601 aagccgcctt tttcaccagc atgttttttt acgattggtt taatactatt tagatcaata
      661 tccataattt tacccctttt taatgttcta atttttgtat tttaaaataa acccaactga
      721 caatttttaa ctttgatatt tgcttcattt gtcatatctc ctactttttt cagtggaata
      781 ttaaggctgt gtcccaataa atgagcttta aaacagctta ttacatcctg gttaaattca
      841 atttcataat cattatcaga atttattatt acatctgcct tctttttaca tttttcagcc
      901 gatttctttt tatctccccc ctttttatca agataatata cgaagttaaa accaacaaaa
      961 tcaataatac tattaccttc tacaattaga taatcataac cttttatttt attaaattct
     1021 tgttctatac cctcttttaa attggctcta tttgatttaa ggtaatatac cttatccgca
     1081 cccgctctgc taaatatctc agtatcagta tctttttcta agatgatctt tttatcatct
     1141 attagttcaa atttatcata tttatcatga atagaacttt taatgacgta tatatttccc
     1201 cttaagttgt taattatctt ttctactaaa gtagtttttc cagaggcttt gctcttaccc
     1261 gaaactaata ttttaaccaa aataataccc ccaatatata aaaaaatcct atgtctaggt
     1321 atgtttttat atttctacct aaaacacagg attccttttt ataatttata tatatattaa
     1381 attttctaat catgtttatc gaacaaatgt cagttatttt gctagatcca tcctttgatt
     1441 tcttctttag taggaacctt gccggttact ttcatttcgc cgtctaaagc aacagccggg
     1501 gttaacatta ctccccgttc agccatttcg ttcatatcct gaactttaat tacttctgcc
     1561 tctaaatcta actcatcaat aacttctctt atattacttt ctgccttctt gcagtttgga
     1621 caacccgatc caaatacctc aaattttctc attaaaatca tctcctcaat ttttttatat
     1681 tacaaacgtt ccgtagataa aaccactaaa ggtagccatt acaaccacca gactaataaa
     1741 taccaaagtc tttttagttc ccattacact tctaatcacc aacatattag gtaaactcaa
     1801 ggcaggtcca gctaacagca aggctaaggc aggacctttg cccattccta aacccatcaa
     1861 gccctgtaaa atagggatct cggttagggt tgcaaagtac ataaatgctc ctaaaattga
     1921 agctgtaaag ttagctagaa gtgaatttcc gccaacaaag ttagatatcc agctggccgg
     1981 tattatacct gcttcattac caggtctgcc catcaaaaat ccggctacaa ttacaccggc
     2041 aaaaagtaaa ggtaagatct gttgagcaaa atcccaggtt gaatcaaccc agtcctttaa
     2101 ttcgttttta gtaaaccagc tttttaattg aataccaagg aacacaagta aagtaatggt
     2161 aaccggccat ttaatactga aaataaatct ccaaaaaccg gtctcactac tcggctttgc
     2221 ccaggctgca aagattaata ttaatactaa ggttacaaag tatattaagt tttgaaaact
     2281 atttctgctt tccttactat catcacccag ggcaaaatca ccttttagtc tttccttatc
     2341 ttcttttcta aaaattatcg ccatcaatac accaattact actgcaaata cgatggctcc
     2401 gactgcccgt gctaatccca gctgccagcc taaaactcgt gctgtcagga ttattgccaa
     2461 tacatttata gcgggtcccg aatataaaaa agcggtagct ggtccgatac cggcccctct
     2521 tttatagata ccggcaaaaa gtggtaagac cgtacaggaa caaactgcca aaactgtgcc
     2581 tgaaactgaa gccacagaat aggagaccca tttctttgct tcactaccaa agtattttat
     2641 gacagcattt ttagaaacga aatttgctat ggcacctgcg ataaaaaaag cagggattaa
     2701 acaaaacaat acatgttcct gggcatactc ttttaccata taaaaagatt ccaaaagagc
     2761 ctgctggaat ttcggattat taattggtac ataaaaagca gccagaaaaa ctgctatgta
     2821 taaagatagt ttttttactt tactcatttt tttcacctca aatattatta gaataaattc
     2881 agcctctcat ttatttcatc ttttatcaca ttatcaacac atgcaaagaa gtttattata
     2941 cagggggttc tcagtgaata atatactttc aaaccttcct ttttcgattt cactactccg
     3001 gcctctttta agatatttaa atgttttgaa accgaagatt ggttcaaatc cagggcttct
     3061 tcgaggtcag atacacacat cttactcttt tttcccagac tatctacaat ctttaatcta
     3121 tatttattgg ccagtgattt catgatcttt tctctcgctt caaataattt ttcttccatt
     3181 gtctacacca ccttatgagt atattaccat attagaatat agttgtcaaa caaaataggg
     3241 gaagcccttc ccctatttat tatatctttt ctatttctat ttcaatcaca tactcatctg
     3301 tatgaaattc gaaaaccatc ttgtattcat cttcagtata tgtaaatcgc attattatca
     3361 cctcgaaata atcattcttt tttcgctatc accaaaaatt ccatatcttt tttgttcacc
     3421 tttctctttc tattaaagtt gccaagttca catccatata tatcaatttg cttaaatcct
     3481 atatttttaa gtctccactt aatttcactg ggagtataat atctttgctt agcttgaata
     3541 gttttttctt taccgttatc atctgccact tctagttcaa attcttccct aaatgttttt
     3601 agatctaagt ttttattgtc ttctgcaatt tgtttaaaga cattgaggac ggttagtatt
     3661 aatttcccgt tatcctttaa tgcattatag gcatttttaa gtatcctgta gtctttttca
     3721 tcatcttcca tcagactaaa ggcaccttca cacaatatta tacttaaatc aaaatcctta
     3781 tgataatcaa gatttaaggc attctttatt ttaaacgtaa catctaaacc tgcctcttct
     3841 cttttccttt tagcctgctc aatcatagac tcagataaat ctatcccagt taccttataa
     3901 cctctttttg ctaactctaa agagtgccgg cctgtgccac accctatatc taatatattt
     3961 ttatctttat tgaaatcaag ttctttttct ataaaatcaa cttcaccttc tgttcctttt
     4021 acaaacggtt cttcttcata acttttacca taatcttcat ataaatcttc ataccatttt
     4081 aatcccacat ttaatacctc cttattattg tatttagttc ttcattaatt tttgccattt
     4141 aatcttgttg gcttttttgg cattgtactt ttcttttaat ttttgatact ctatctcctt
     4201 tttcattttg aagtagttat ttaatctatc ttcacttaac ttaccatctt ttacagcatt
     4261 tttaacttca cagcctggtt catgggtatg agtacagtca ttaaacttac aatctctcgc
     4321 caactgttct atctcactaa atacattatc taatgaattt tcatccaccc agagttgtat
     4381 ttctctcata ccgggggtat caatgataac tcccccgtca ggtactaaaa aaagttctct
     4441 attagtagtt gtgtgtcttc cccgcgcatc atctgccctt atatccttta tatcctgttt
     4501 cttttcttct aacagcagat tgattatgga agacttacca acgccggaag aaccgactaa
     4561 aactgctgtt ttatcgggat ttaatatttc ccttattttc tcaatacctt cattgttaac
     4621 acaacttgtt attatatatt cagtaccaaa agcaatatta ttgattttat caatataata
     4681 ctctctatca tcacataaat ctgatttatt taaaataatt atcggtttgg ctccactatc
     4741 ccaggcaatc gtcatatatc tttctaaacg gcggggatta aagtcctgat ttaaagaagt
     4801 aacaataaaa attatatcga tatttgccgc tataatttgt tctgcctctt ttttaccggc
     4861 ctggtttctg gagagctgac tctttctggt taagacttct ttaattgaac cattactttc
     4921 tttgttatac tgatcaaaga ttacccagtc ccccactact ggaaagttac ctttactgga
     4981 cttaaagtaa ctactaccag ataaagtggc cattatttct ccctcagtag ttataattct
     5041 atataaatct ctatactgag ctgagactct tgctaattta tttttatcct ctatattata
     5101 ttcaaatcta tttaaaccta atttattaaa attcattttt agctcccttc ttatctaaaa
     5161 acaaaaaacc acaggtacaa taaacctgtg gtaatataaa ctcatttata ttattttttc
     5221 acctaaagtt aaacacagag ttcattaatc atcagttcta atcccgccta aagtcagtgg
     5281 gcatagtaat ccccgggatt aaaacacacc atcttcagtt tgatgagatt aaagaactct
     5341 cctcttcatt aaaacatcac tcactttaat attattttca catttaatat tataaccaaa
     5401 caatgaaata atgtcaacaa aaaattgtat tttttcatga atttatctaa attttttaaa
     5461 aaaattaaat ttaacctcgc aagaattaat taatcgatga cagctgtact ccaatttgat
     5521 atttttaacc agattaatcc caagagagat ccccccagat gggaaagggc aactgcccac
     5581 cagagaccca cctgagacat agaaaaaatt gtaattaata aataggctat acctaaccta
     5641 aacacccata aagatgaaga agatattacc ataggtggaa ccgttttacc ggctccctta
     5701 aaaacaccat tgcccacata catagctccc aaaaatccga aggtcaaagc tacaatcctt
     5761 aaatagctgg aaccatattt aactacggct ggggcatcat tgaatatttt aattacaaat
     5821 ctgggttcaa aaaatagtac aatacctaaa atactcatgc taataaatat gatacctata
     5881 ctgaccaggg cggttttttc agccctttcc tttttatctg ccccaatgtt ttgaccgacc
     5941 atcgtggtgg cagccgctgc taccccaaaa gctaaaatag ttggtaaact aataatccta
     6001 ttgacaatcc cgtaagcagc caaagtcatg gtcccaaaac cggcaactaa actggtcatg
     6061 agcacctgtc ccaaagaaat catagactgt tcaactgaag aaggtactcc tattcttatt
     6121 atctttttga taatactaaa atcccatttc aggttagata actttaaata aaatccatct
     6181 acaccctttt caataattat taaggcataa atggccacta tcgcgcgggc aataatagtt
     6241 gctagagcag cccccactac ccctaactcg gggaaaaagc caatcccaaa aataaataaa
     6301 ggatctaaaa taatattaat ggttacagaa aagaacatca taattgaggg tgtttttgta
     6361 tctccaatcc cccttaatat tgaagagaaa atgaagaata taaacataaa gggcatacct
     6421 gtaaaaatgg tcctaagata tccggctgca tcaggcacta tttccggttc tgcccccatc
     6481 aatatgataa tatcgttagc taaaacagat cctactatac tgattacaat tgctaaaaat
     6541 actataaatg aaaacagctg accgataata ttatttatcc ttttcacatt accgcgtcct
     6601 ttatgctggg ctattaaagc ggtaccggca atagtgagtc ccgctgctat tgaaatcatc
     6661 ataaagacaa tcggaaagct aattgaaata gcggcaaccg cttccggacc tactctacct
     6721 aaccagagcg tatccgccag gttatataag gtctgcatca attggcttaa cataatgggc
     6781 caggctaaag taattaaaga tttcaaaata ggcccttcaa taatatattt atattttgct
     6841 gaatgttttt caatttcttt caaaaaaatc aactccattg actgctatga acttaactag
     6901 gaacaatccc gcttccgcgg gattgtttta atcatcataa tttctttttg aaatatattc
     6961 aaaatagaaa ataagtaaac ctattcctat aattatcatc tcaaaaacta agataaccat
     7021 ccctatatta tttgccattg ccgcaaacca ggccggcata ttctcactca ttttttggcc
     7081 tgtttgcaca aagtgggcca ttgcaacccg gcctgaggca gccaaggatg aaagggcaac
     7141 aatccctatt gaagcaatgg ctgaaatact gattcccccg attgctaata aaaaggcaaa
     7201 ggctaaaccg gccacggaaa atactccggc tgataaaaag ccaaaagaaa gcagcccaat
     7261 acttaaccct ccgattgcaa ttaaaccata ggctaattta ccaaaggcaa ttaccccccg
     7321 ggccttactt ccaatcgcga ttataccttt ggctacatca tagtcgcctt tttctctatt
     7381 gaaaccactg gtcagatgaa aaacagggag gttgcctatt tccatcctac tttttatatc
     7441 tacatgaggt actttggaag aaaacttaat ggcattaact aaacccatat cttcaatata
     7501 gtccgcattt tctcgggtta aacgaagtgc atagggtatg gccccttcca tctcctcgtt
     7561 atatgcctta gcttcatcat catatctact taaaatatca tctatttcag cctcaacttg
     7621 tttgataatt tttttactct ttttactatt tagatatagg ttctttctaa aaattttcat
     7681 gtatttcttc ttgatgtcca tcatttcacc tcaagtagtt tatattaaat ttcaattaat
     7741 tcgtacccgg tttctcccag accaagttct tctccatagg tcaattggat tgtaccctca
     7801 gtatgatcac gagaaccttt aaacttatct tcacccggtt tatagttttt ctctaagtga
     7861 gaatgtctta atccttcctg tttattaact aaatcataac ttgctttatc cagggcaatg
     7921 gggtctttag aagctaaaat tccaatatca cgaactaggg gagcatcact aaagggagca
     7981 cagtcacatt cgggcgtaat atcaagtagg aagttgatat atccgacttt atccaatttg
     8041 tttttaaccg ctccatatgc atattcagtc attctttcca taaatggcct tatttcagac
     8101 tcccagtcca taccgattgc atcaacagga caggccgtaa tacattctcc acacccgata
     8161 cattttttag gatcaataac tgcgaaatct tcgaccacaa tcgcgttttc cggacaaact
     8221 tcagcacagg ttccacaggc gatacactta tcattatctg attcaatatc tacactatgc
     8281 tgatcttttt taccggcagc tggtgcacag cccatcgcta tatttttgat agatccccca
     8341 aatcctgcaa tttcatgtcc cttgaaatga gacattacaa tcattccgtc cgcatggaca
     8401 aaatcagaag ctatcttgac tttattgaaa tgtttagcac caatttccac ctctaaaaag
     8461 ttttggcttc tcaacccatc tgcgataata actggtgcat ctaagacaga aaagttaaaa
     8521 ccgtgtagat ttgctaccgt atggtgatct atagaattat gtctcatccc tgaatatagg
     8581 gtatttgtgt cggccacaaa tggtttacct ttatgttctt ttattttatc tactacttga
     8641 cgggcaaata ccggatttag ataaccgtca ttaccgtgtt caccaaagtg aattttaaca
     8701 gcagtaaaat cttcttcctc aattaaatca ccaaaaccag cttcatcgaa aagctttttt
     8761 atctttttga tcttacttgt gccttcactt ctagctctta tatcagcgaa gtatacttta
     8821 ctcattaata attccccctt tattacaatt attttatatc tttttatgta ttaatttttt
     8881 tcatttattc caaatcatat caattttatc tgataatata ttttgttcca tctttacagg
     8941 ccacatttcc ttgattgaat cttctctcat tgcagccgca atattatgaa agatctgttt
     9001 attaaacttt attttatcta atattgtttt aatttcttct ctttttgtat ttttatcata
     9061 aggacatttc acaaaactat catcatactc tattctattc ttagcagccc taatcctctt
     9121 ttctcttaaa taaattagag gtctaattat gtagacctca ttattactta aaaatctttt
     9181 gggttgtaag gtattaacct gtcctgaata caaaatactc atcagaaagg tttctacagc
     9241 atcatcataa tggtgtccaa aggctaattt ttcaaagccg ttagccttca tataatcaac
     9301 aagtgctcct tttttaaagt gagcacattt tgaacagggg ttattacttt ct
//
LOCUS       DATGCJ010000011         7722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_46162, whole genome shotgun sequence.
ACCESSION   DATGCJ010000011 DATGCJ010000000
VERSION     DATGCJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46162"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="mnmA"
                     /locus_tag="VKN64_00520"
     CDS             complement(<1..859)
                     /gene="mnmA"
                     /locus_tag="VKN64_00520"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMA58745.1"
                     /translation="MQKVLVAMSGGVDSSVAAAYLKEQGYEVIGLTFIMFSEKNSLSQ
                     KDRQAVNDAKDVADQLDIEHYTSNITREFKDTIIDDFIEQYKQARTPNPCVICNRKIK
                     FNFLLDKVKEFKADYMATGHYAKIEHNKRHLLKRAKYEDKDQSYMLYRMSQAQLEKTL
                     FPMGDFSKEQIRDMAKNYNLEIHDKPDSQDICFIKDNNYIEFLDSQVQDIGKPGPILD
                     LEGNKLGEHDGLHHYTIGQRRGLGISKPYPLYVVKLDIGNNAVIVGKNKDVFRNSFKI
                     IEPNWVYYEK"
     gene            complement(872..1297)
                     /locus_tag="VKN64_00525"
     CDS             complement(872..1297)
                     /locus_tag="VKN64_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMA58746.1"
                     /translation="MKVSTRGRYGLRAMIELAKNENSGAIPLRIISEKQNISEQYLEQ
                     LFVNLRNSGLVKSVRGANGGYLLNKAPEDITVEEVLVALEGPLNIVDCIADESLCDFS
                     KECATHELWIELKERIEDLLVNITLEDLKQRAIKLQKEN"
     gene            complement(1389..2015)
                     /locus_tag="VKN64_00530"
     CDS             complement(1389..2015)
                     /locus_tag="VKN64_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HMA58747.1"
                     /translation="MDLQIKIPDSTSEVKNKVKDSIFWGNIKHVKSKEEAEQFITTIK
                     EKYHDASHNVSAYIILKDEDNIKYYDDDGEPSKSSGPPVLETIKGENLVNTVIVVTRY
                     FGGTKLGIGGLIRAYGGTAKQVIQKAGIKKLDLYYYLKAKGNYQIVGTMLGQVEAFKG
                     KIKETKYSEKGAEIYFYLDPEKYTNLKNQLIEQTGNKVEFEILKKLYL"
     gene            complement(2020..3351)
                     /locus_tag="VKN64_00535"
     CDS             complement(2020..3351)
                     /locus_tag="VKN64_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMA58748.1"
                     /translation="MPNLFENDKNNDNHQQPLAYRMRPKSLDDIYGQADILGENKILR
                     RAIEADQIQSLILYGPPGTGKTSIASVIAENTDSDFKVLNAVTSGVGDIRDIIKEAKE
                     KKDLYNTRTILFIDEIHRFNKAQQDALLPAVEKGIVIMIGATTENPYFEVNSPLLSRS
                     RIFQLEPLKKEDIINIINDALNSKMGMADYNIGIDEDTIDFIAKISEGDARVALNTLE
                     SAVLTTPVNGEGKILITKDIIREALQKKSLNYDKKGDNHYDNISALIKSMRGSDPDAA
                     LFWLAKMIEAGEDPKFIARRIIIHAAEDVGLADPQALLVANAAFEAVTKVGLPEARIP
                     LAEAVIYIASAPKSNTAVKAIDKALDFVRNNPTGTVPVHLRSSNYKGAKDLGRGIEYK
                     YPHNYKNNYVKQNYFPEEIDKQKFFEFSDLGNEKRLRDFLKKLKDKDNEPG"
     gene            complement(3356..3937)
                     /locus_tag="VKN64_00540"
     CDS             complement(3356..3937)
                     /locus_tag="VKN64_00540"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HMA58749.1"
                     /translation="MSLKEELREKYFEKRQNLSDEFIKNKSEVIINKLDEYVQEHDYK
                     DIMIFVSFRNEVYTHGIIKKWLKDNSKNIYVPYIDKNLDEMKVSKIQDFAKDLKPGVF
                     DILEPRKELKDNTSVKNIELIIVPGLVFSKQGYRIGYGGGYYDQFLSKVEANVDKVGI
                     TCSEFIVNELPVDQHDLPVDKIITDQNIIDAER"
     gene            complement(4014..4192)
                     /gene="ssrS"
                     /locus_tag="VKN64_00545"
     ncRNA           complement(4014..4192)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VKN64_00545"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4227..6008)
                     /gene="aspS"
                     /locus_tag="VKN64_00550"
     CDS             complement(4227..6008)
                     /gene="aspS"
                     /locus_tag="VKN64_00550"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMA58750.1"
                     /translation="MLGKLEGYTRTHKCGELRIDDEKNEITVMGWVQKRRDHGGVIFI
                     DLRDRSGFVQIVFNPDINKDAFDKAEELRSEYVIAVKGQVRKRPTGNINDELPTGKIE
                     IEGHQLKILDKAETTPFEIEDNVNPGENLRLKYRYLDLRRPMMRDMMELRHRVVKKTR
                     DYLDENDFLEIETPLLTKSTPEGARDYLVPSRINPGHFYALPQSPQLFKQLLMVSGME
                     RYFQIAKCFRDEDLRANRQPEFTQIDIEMSFVTREDIFEITENLLREVFAIQDIELPD
                     EIPVMTYQEAIDQYGLDKPDLRFDMKLKDISDIVKDSDFNIFSGTIKKGGQVKGITVK
                     GGAEFSRSKIDELEDFVKLYEAKGLAWIKITEDGVNSAIAKFLSDEEIEKIINKMEAA
                     QGDLMLFVADKPNIVATALGQLRVHLGEKLDLIDKDKYEFTWIIDFPLFEYKEEEGRY
                     AAKHHPFTAPLPEDIDKLDGELDKVKANSYDIVLNGEELGGGSIRISNKKLQHKIFDA
                     MNFTEEEIENKFGFLMDAFEYGTPPHGGIALGLDRLIMILGNTDSIRNVIAFPKTQKA
                     TSPLTDAPSIVNKKQLDELHIKLVDLD"
     gene            complement(6023..7279)
                     /gene="hisS"
                     /locus_tag="VKN64_00555"
     CDS             complement(6023..7279)
                     /gene="hisS"
                     /locus_tag="VKN64_00555"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMA58751.1"
                     /translation="MINAPRGTNDIIPPESAKWNYIEEKAKYIFDLYNYDEIRTPIFE
                     YTELFQRGIGEVTDIVEKEMYTFEDKSGRSITLRPEGTASVVRSFLENKIYGKAQPTK
                     YYYIGPMFRYERPQAGRYRQFNQLGIEVFGTKDPMVDAEVIAVGMHYMDILGLEDLTL
                     YINSIGCPKCRPQYLEKLKKYLNENKEALCEDCKHRIETNPLRVLDCKNEGCQLVIED
                     APKIIDHLCDECEVHFESLQENLKNIDIEYEIDPNLVRGLDYYTKTAFEVKYEGLGAQ
                     DTIFGGGRYDKLAEEIGNKDIPGIGFALGIERLLLTLEKQEIEIPVDKSIDIYLTTIG
                     DKAKKACFELLDEFRKNNIKAEIDYLGRSVGGQMKSADRMNATYTVIIGENELNSGKA
                     TVRNMKNGEEQEIKLDTLVESIEKMI"
     gene            complement(7318..>7722)
                     /locus_tag="VKN64_00560"
     CDS             complement(7318..>7722)
                     /locus_tag="VKN64_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA58752.1"
                     /translation="SIYTYLAQAYAGAEQYDKALETFNTALEDYPENWELRLNFGEYY
                     ANRENKEKAIEQYNKASELAGDNFYAHYKLFMAFNNIGANKEAEKEMQRITEIQENMQ
                     SQQQQQQSEQPEDATSIEEEIEQTKPESENDQ"
BASE COUNT         2192 a         1587 c         1050 g         2893 t
ORIGIN      
        1 atttttcata gtaaacccag tttggctcaa ttattttaaa agaatttctg aaaacatctt
       61 tgtttttacc tacaattacc gcattattac caatatcaag tttcactaca tataagggat
      121 agggctttga aatacctaaa ccccttctct ggccgatagt ataatgatgt aaaccatcat
      181 gttcgcccaa cttattacct tctaaatcta aaattgggcc tggtttgcct atatcttgga
      241 cctgactatc taaaaattct atataattat tgtccttgat aaagcatata tcctgactat
      301 cgggcttatc atgaatttct aaattataat ttttagccat atctcttatt tgttctttgg
      361 agaaatcgcc catcggaaat aatgtttttt ctaactgggc ttgtgacatt cgatagagca
      421 tgtatgattg atctttatct tcatactttg cccttttcaa taaatggcgt ttattatgtt
      481 ctatcttggc ataatgaccc gtagccatat aatcagcctt gaattctttg actttatcta
      541 ataaaaaatt gaatttaatc ttcctattac atataacaca gggattggga gtccttgcct
      601 gtttatactg ctctatgaaa tcatcaatta tagtatcttt aaattccctg gtaatattac
      661 tggtataatg ttcaatatcc agttgatcag ctacatcttt ggcatcatta acagcctgtc
      721 tgtctttttg acttaaagaa tttttctccg aaaacataat aaaagttaaa ccaataactt
      781 catatccttg ttcttttaaa taagcagccg ccacagaact atcaactcca ccgctcattg
      841 caactaaaac tttttgcata atgaaactcc tttaattttc tttttgcagt ttaatggctc
      901 tctgttttaa atcttccaag gtgatattaa caagaagatc ttcaattctt tcttttaatt
      961 caatccataa ctcatgagta gcacattctt tagaaaagtc acataaagat tcatctgcta
     1021 tacaatccac aatattcaag ggtccttcca aagctaccag aacttcttcc acagttatat
     1081 cttcaggagc tttatttaat aggtaaccac cgtttgctcc tcttacactc tttacaagac
     1141 ctgaattcct taagtttaca aataactgtt caagatactg ttctgatatg ttttgttttt
     1201 ctgaaattat tcgtaaagga attgcaccag aattttcatt tttggcaagc tcgatcattg
     1261 cccttaaacc atatctacct cttgtagaaa ctttcatatt tttaatacct ccattatata
     1321 gatgagtaaa cctatcacta tattatattt taccatttca tacttattat atcaattaga
     1381 tataaatttc acaaatataa ttttttcaaa atttcaaatt ctactttatt tcctgtctgt
     1441 tcaattagct gattttttaa attagtatat ttttcaggat ctaagtaaaa atatatttct
     1501 gctccttttt cactatactt agtttctttg attttacctt tgaaagcttc tacctggcca
     1561 agcatggtac caactatctg ataattaccc ttggctttta ggtagtagta taaatctaat
     1621 ttttttatcc cagctttttg aataacctgt ttggctgtgc ccccataagc tctgatcagt
     1681 ccccctattc ccaacttggt tccgccgaaa taacgggtaa caactattac tgtatttact
     1741 aggttttctc ctttgattgt ttctaataca ggaggacctg atgatttgga aggctctcca
     1801 tcatcatcat aatatttaat attatcttca tcttttaaaa ttatgtaagc actcacatta
     1861 tgtgaagcat cgtggtattt ctctttgata gtagttatga actgttccgc ttcttcttta
     1921 gacttcacgt gcttgatatt accccagaaa atactgtctt tgactttgtt tttaacttct
     1981 gaggtagaat caggtatttt tatctgcaaa tccattttat cacccgggtt cattatcttt
     2041 atctttcaat ttttttagaa aatcccttaa ccttttttca ttacctaaat cactgaactc
     2101 aaaaaatttc tgtttatcta tttcttcggg gaagtaattt tgctttacat aattgttttt
     2161 ataattatgg gggtatttat attctattcc cctccctaaa tccttagcac ctttataatt
     2221 agaagatctt agatgcacgg gtactgtacc ggtaggatta tttcttacaa agtccaaggc
     2281 cttgtctata gctttaacag cggtattact tttgggagct gaggcaatat atataacggc
     2341 ctctgccagt ggtattctcg cttccggtaa accgacttta gttaccgcct caaaagctgc
     2401 attagccacc agaagggcct ggggatctgc taaacccaca tcttcagctg cgtgaataat
     2461 aatccttctg gcaatgaatt taggatcttc tcctgcttct atcatctttg ccaaccaaaa
     2521 aagggctgca tcaggatcag aaccgcgcat acttttgatc aaggcagaga tattatcata
     2581 gtgattatca ccttttttgt catagttcaa tgattttttc tgaagtgctt cccttataat
     2641 atccttagtt attaagatct taccctcccc attaacggga gttgttaata cagccgattc
     2701 caatgtgttt aaagctactc ttgcatcacc ttctgaaatt tttgcaataa aatcaatagt
     2761 atcctcatct atcccaatat tataatcagc catacccatt tttgaattta gagcatcgtt
     2821 aatgatatta ataatatctt ctttctttaa aggctctaat tgaaatatcc tagacctcga
     2881 taaaaggggt gaattgactt caaaatatgg gttttctgtg gttgcaccga tcataattac
     2941 aatccctttt tctacagcgg gtaataaagc gtcttgctga gctttattaa atctatgtat
     3001 ctcatctata aacagtatcg tccttgtatt atagaggtct tttttctctt tagcctcttt
     3061 tataatgtcc cttatatccc ccacccctga ggttaccgca tttaaaactt taaaatccga
     3121 atcagtgttc tctgcaatta cggaagcaat actggtctta ccagttcccg gaggaccata
     3181 aagtattaaa gattgtattt gatcagcttc aattgctctc ctcaaaattt tattttcacc
     3241 tagaatatcg gcctgtccgt agatatcatc gagagatttt ggtctcattc tgtaagccaa
     3301 aggttgttga tgattatcat tatttttgtc attttcaaaa agattgggca aaatatcacc
     3361 tctcagcatc aattatattt tggtcagtaa taattttatc aacaggtaga tcatgttgat
     3421 ctaccggtaa ctcatttact ataaattctg aacaagtaat accaacttta tcaacattgg
     3481 cttctacttt gcttaaaaat tgatcataat aacctccgcc atagccgatt ctgtaaccct
     3541 gtttagaaaa gactaatccg ggtacaataa tcaactcgat atttttaaca gaggtattat
     3601 cttttaattc ttttcttggt tctaatatat cgaatacacc cggttttaaa tcttttgcaa
     3661 agtcttgaat tttacttact ttcatttcat ctagattttt gtctatataa gggacatata
     3721 tatttttact gttatccttt aaccattttt taattattcc atgggtataa acttcatttc
     3781 taaaagaaac aaagatcata atatctttat aatcatgttc ctggacatat tcatccaatt
     3841 tatttattat cacttcactc ttgtttttta taaattcatc ggacaaattt tgtctttttt
     3901 caaaatattt ttcccttaat tcttctttta agctcaattt taatcacctc tgtaaagcta
     3961 ttatataata taatattctt aaatcaaata aaaaaatccc caattaaggg gataaagaat
     4021 ccaccgtgcc gtaggtatca tttaattttg atccctttcc gccaagtggg tgccctccta
     4081 tctggtttgt atgtcctgct agaggcatat acgctgcaga tagagtccag gctccctttc
     4141 aaagagtgtt ggtcaaaatt ttctataacc cttacgcaca cagtagaatt tctttaatta
     4201 tatattacca taaacttttt ggtatttcaa tccaaatcaa ctaacttgat atgtaattca
     4261 tcaagctgtt tcttatttac tattgaagga gcatcggtta aaggtgaagt agctttttgt
     4321 gttttgggaa aagcaataac atttctaatt gaatctgtat ttcctaaaat cattattaac
     4381 ctatctaaac caagggcaat acctccatgg ggaggtgtcc catattcaaa agcatccatt
     4441 aaaaatccga atttgttttc aatttcttct tccgtaaagt tcattgcatc aaagatttta
     4501 tgctgtaact ttttattact tatcctaatg cttcctcccc caagttcttc accatttaat
     4561 actatatcat atgagttagc cttaacttta tccagctccc catctaattt atcaatatcc
     4621 tctggtaaag gggctgtaaa ggggtggtgt ttggctgcat atctaccttc ttcttccttg
     4681 tactcaaaaa gtggaaaatc tataatccag gtgaactcat acttatcttt gtcaattaaa
     4741 tcaagtttct ctcccagatg aactcttagc tggcctaagg ctgttgcaac tatatttggt
     4801 ttatcagcta caaaaagcat taagtcgccc tgagctgctt ccattttatt gataattttt
     4861 tctatctctt cgtctgataa gaattttgca atagctgaat taaccccatc ttcagtaatt
     4921 ttaatccagg ccaggccttt agcctcataa agttttacaa agtcttctaa ttcatcaatt
     4981 ttactgcgtg aaaactccgc tcccccttta actgtaatac cttttacctg accacctttt
     5041 ttaatagtgc cactaaagat attaaagtcg ctatctttta caatatcaga gatatctttt
     5101 aacttcatat caaaccttaa atcgggttta tcaagtccgt attgatctat agcctcctga
     5161 tatgtcataa ccgggatttc atctggtaac tctatatcct ggattgcaaa tacttctctc
     5221 aatagatttt ctgtaatttc aaaaatatcc tccctggtca caaaagacat ctcaatatca
     5281 atttgggtaa attcaggctg cctattagct cttaaatctt catccctaaa acacttggca
     5341 atttggaaat atctttccat accagaaacc atcagcagct gcttaaacag ctggggagac
     5401 tggggtaatg catagaagtg tccagggtta attctactgg gtacaaggta gtctcttgcc
     5461 ccttctggag tacttttagt taaaagaggt gtctcaatct ctaaaaaatc attctcatct
     5521 aaataatcgc gggtcttctt tactacccga tgtctgagtt ccatcatatc tctcatcatc
     5581 ggtctgcgaa gatcaagata cctatatttt aatcttaaat tctcaccggg atttacatta
     5641 tcttcaattt caaaaggagt ggtttcagct ttatctaata ttttaagctg gtgtccttct
     5701 atttctattt ttccggtggg aagttcatca tttatattac cggttggtct tttccgtact
     5761 tgccctttaa cagcaatcac atactcagat cttagttctt cagccttatc gaaggcatct
     5821 ttattaatat cgggattaaa gacaatctgg acaaaacctg agcggtctct cagatctata
     5881 aagataactc ccccatgatc tcttcttttt tgtacccaac ccataacggt tatttcgttt
     5941 ttttcatcat ctattcgtag ttcaccacat ttgtgggtcc ttgtatatcc ttctaatttt
     6001 cctaacactt ataatgcctc ctttagatca tcttctcaat actctctact aatgtatcta
     6061 actttatctc ctgttcctcg ccatttttca tatttcttac tgtagcttta ccgctgttta
     6121 attcattctc accaataatt acggtgtaag ttgcgttcat tctatcggca gacttcatct
     6181 gtccacccac acttctacct aaataatcta tttcggcttt tatattattc tttctaaact
     6241 catcaagcag ctcaaaacaa gcttttttag ctttatcacc gatagttgtc agataaatat
     6301 cgatactttt atcgacaggg atctcaatct cctgtttttc taaagttaat agtaatcttt
     6361 ctattcctaa agcaaatcca ataccgggaa tatctttatt accaatttct tcggctaatt
     6421 tatcatatct accaccgccg aagatcgtat cctgggcacc caaaccctca tatttaactt
     6481 caaaagctgt ttttgtataa taatccaagc ctcttactag attaggatct atctcatatt
     6541 cgatatctat attttttaaa ttttcctgta agctttcaaa atgtacctca cattcatcac
     6601 agaggtgatc tataattttg ggagcgtctt ctattaccag ctgacagccc tcatttttac
     6661 agtctaaaac ccttaatgga tttgtctcaa ttcgatgttt acaatcttca cagagagctt
     6721 ctttattctc attaaggtat ttttttaatt tttctaaata ttgaggtcta cattttggac
     6781 aaccaattga attgatataa agagttagat cttctaatcc caatatatcc atataatgca
     6841 taccaactgc tataacttca gcatcgacca tgggatcttt tgtgccgaat acctctatac
     6901 ccagttgatt aaactgtcta taacgcccgg cttgtggcct ttcatatcta aacatggggc
     6961 caatataata atatttggtg ggttgggcct ttccataaat cttattttct agaaaagacc
     7021 tcaccactga agcagtcccc tccggtctga gtgtaatact tctgcccgat ttatcttcaa
     7081 aggtatacat ctctttttca actatatctg ttacttctcc gatacctctt tgaaataatt
     7141 ctgtatattc aaagatgggg gtccgaatct catcataatt atataaatca aagatatatt
     7201 ttgctttttc ttcaatataa ttccatttcg ccgattccgg cggaattata tcatttgtac
     7261 cgcgtggagc atttatcatt atatttcacc cttatttact tattaataaa attaacttta
     7321 ctgatcattt tcactctcag gttttgtttg ttcaatttct tcttcaattg aggtagcgtc
     7381 ttctggctgt tcagattgct gttgttgctg ctgactctgc atattctcct gtatttcagt
     7441 tattctctgc atttcttttt ctgcttcttt atttgcacct atattattaa aagccatgaa
     7501 taacttataa tgggcataga aattatctcc ggccagttca gaagccttat tatactgctc
     7561 aattgccttt tctttattct ccctgttagc ataatattcg ccgaagttta atcttaattc
     7621 ccaattttca ggataatctt ctaaagcagt attaaaagtt tctaaggctt tgtcatactg
     7681 ttcagcccct gcataagcct gggctaaata agtatatatg ct
//
LOCUS       DATGCJ010000012         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_1460, whole genome shotgun sequence.
ACCESSION   DATGCJ010000012 DATGCJ010000000
VERSION     DATGCJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VKN64_00565"
     CDS             complement(<1..1909)
                     /locus_tag="VKN64_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58753.1"
                     /translation="MIEVFLNNEKIDLYKDENIQVNDSIQNIKDIGKIFTTFSRQFSV
                     PATKRNNKIFKNFYNFDIIDGFDPREKVRSVITKNGATWRKGFIQIENVKLKNNKPSS
                     YKIFFTGEISELKDIFKEDRLSDLSYLSNFDHSYTQDNVEAGFINYLSIVNGNVTSTF
                     FKNDICYPFISVGSRYAYNNSGQLKKVNENGNFSGSNLNYRDLKPAIRLTRIIEAIEA
                     EYNMTFSNDFFGTDDFTGLYLWLNRTLGQIGAETDIVIELFNNEFDSSFDNLLQVFTN
                     SNPIAFRRYELTYTIIAQGTGSVNYEVINNFNGEELTAGTHEFDNDTFEIFKLLQLAS
                     PDSGINSENYVPLLILRSENNAITQLDVQLKIKRREFSGNPQDDTEEETQTSDFLVFN
                     AVNEIRINDLIPKMKVIDFLTSIFKTFNLTARVVDGVIEVKSLDSFYDDGGTQDISSL
                     VDIEDVTVSRVETIKEFNFEFNQSESILIKERNKRVDDEFGNLDFILENEDPTKVIGG
                     INYNIKSEFYKILFEKLTIGDTGQPTNTLFSWYVDEKKEPISNEPIIFYTDRKQANGI
                     TFQGSNTTPATYIAGLNAIGNKSINFGSEIDEFTLQLNNNSLFNNFYKNYIERVVSPQ
                     ARKVSLTAILRD"
     gene            complement(1906..2040)
                     /locus_tag="VKN64_00570"
     CDS             complement(1906..2040)
                     /locus_tag="VKN64_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58754.1"
                     /translation="MYWIDDEPAILTDSKWTEKTKVNDKLINYDFDFDFANDDINSIR
                     "
BASE COUNT          646 a          384 c          310 g          837 t
ORIGIN      
        1 tatcccttaa gattgcggtt agcgaaactt tacgagcttg tggactgact actctttcta
       61 tgtaattctt ataaaaatta ttaaataaac tattgttatt aagttgcaag gtaaactcat
      121 caatttcact cccaaagttt attgacttat tgcctatagc atttaatcca gctatgtaag
      181 tggctggtgt agtgttgcta ccttggaatg ttataccatt agcttgtttt ctgtcagtgt
      241 aaaatattat tggttcatta gatataggtt cttttttctc atcgacatac caactgaata
      301 gagtatttgt tggctgtcct gtatctccta tggttagctt ttcaaaaagt attttataaa
      361 attcagattt aatattgtag tttatgcctc ctatgacttt agtagggtct tcattctcta
      421 agataaaatc taaatttcca aattcgtcat ctactctttt attgcgttct ttgattaaaa
      481 tagattccga ttgattaaac tcaaagttaa actccttaat agtctccacc ctagatactg
      541 ttacgtcttc aatatctaca agactgctta tatcttgagt gccaccatca tcatagaaac
      601 tatctaaact ttttacttct ataacaccat ctacaacccg agctgttaaa ttaaatgttt
      661 taaaaataga cgttaggaag tctataactt tcattttagg tattaaatcg tttattctta
      721 tttcattgac tgcattgaaa acaagaaaat ctgatgtttg tgtttcttct tctgtatcgt
      781 cttgaggatt accgctaaac tcccttcttt taatttttaa ttgtacatct aattgcgtga
      841 ttgcgttatt ttcacttctt aaaattaaaa gcggtacata gttctcagaa ttgattcctg
      901 agtctggaga agcaagttgt aatagtttaa aaatttcaaa ggtgtcatta tcaaactcat
      961 gcgttcctgc tgttaattct tcaccattaa aattattaat aacttcatag ttaactgacc
     1021 ctgtgccttg tgcaattatt gtatatgtta attcatatct cctaaatgca atggggttgg
     1081 aattagtaaa aacctgtaaa aggttatcaa aactactatc aaactcatta ttaaatagtt
     1141 ctataactat gtcggtctca gctcctattt gccccaaggt cctgtttagc cataaatata
     1201 atcctgtaaa gtcatcagtg ccaaaaaagt cattagagaa tgtcatatta tactctgctt
     1261 ctatagcctc tattattcta gtcagtctta tagctggctt taaatctcta tagtttaaat
     1321 tgcttcctga aaaattgccg ttttcattta ctttcttgag ttgcccactg ttgttataag
     1381 cgtatcggct gcccactgaa ataaatggat agcaaatatc atttttaaaa aaagtagatg
     1441 taacgttacc gttaacaata gaaagataat tgataaaacc tgcctcaaca ttatcttgtg
     1501 tgtaactatg atcaaaatta gataaataag acaagtcgct taatcggtct tctttgaata
     1561 tatctttcag ctctgatatt tctcctgtga aaaatatctt gtaacttgat ggtttgttat
     1621 ttttaagttt tacgttctcg atttgaataa aaccttttcg ccacgttgcc ccatttttcg
     1681 ttataactga ccttactttt tcccttggat caaaaccatc tataatatca aagttgtaaa
     1741 agttcttgaa tattttatta tttcttttag tggctggcac gctaaactgc cttgaaaatg
     1801 tagtaaagat tttgccaata tctttaatat tctgaataga gtcattcact tgtatattct
     1861 catctttata caagtcaatt ttttcattat ttaaaaatac ctctatcatc ttatactgtt
     1921 tatatcatcg ttagcaaagt caaagtcaaa gtcgtaattg attaatttgt cattaacctt
     1981 agtcttttct gtccatttgc tatcggttaa gatagctggc tcatcgtcaa tccagtacat
     2041 ctcactgtac aatagttgct taaatacggt gttaaaactt tcaggataaa aaccactcga
     2101 tatttttacg cttgcctgcg cttgtttgtt gtatactcta cgttgtccta gatgcggata
     2161 tacttgtccg tttgtta
//
LOCUS       DATGCJ010000013         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_537632, whole genome shotgun sequence.
ACCESSION   DATGCJ010000013 DATGCJ010000000
VERSION     DATGCJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537632"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VKN64_00575"
     CDS             <1..286
                     /locus_tag="VKN64_00575"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HMA58755.1"
                     /translation="KFSEKTLSLFKQVFGHKIKSFELQLLNKYDLPDEVIRKGIELSG
                     THNAQSLQYLLDILKEWSSRGITTLEGIERYIRQFKGQETDEDLEAKGYR"
     gene            717..884
                     /locus_tag="VKN64_00580"
     CDS             717..884
                     /locus_tag="VKN64_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMA58756.1"
                     /translation="MKFENIEITKRLNKTKVDTQGKVYIPAKIRELCGIEKNSEVNIQ
                     VDKDSKAVLIR"
     gene            894..1112
                     /locus_tag="VKN64_00585"
     CDS             894..1112
                     /locus_tag="VKN64_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58757.1"
                     /translation="MKWGEVKKGQLVKIGPSNFVGYIYKLDHKEKMVKVEWTGSWCDW
                     NKPEKLDLIEISDFNHFIKGWQNARKDY"
     gene            1262..2464
                     /locus_tag="VKN64_00590"
     CDS             1262..2464
                     /locus_tag="VKN64_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HMA58758.1"
                     /translation="MSRKGYHLLPLKPGDKIPYFDLLPGNSTDIFKENQAKSSLVESW
                     IKQDPQVNLGVFAGTEISKNYRLVIVDFDKEMNFGLPITPMVKTKRGYHAYFKCRTDR
                     LPEAHKTPRGEIKTSGYVVAPPSKHPSGMVYKWADYLSFIDVGLAEFHQRSEQIISFL
                     EGTNSDRFYYNQRDEDKLNTTININSSVKNRDPLSISKDIKENRKKLEQLSRSEEVAL
                     SVMREVFEIEVGRVGRTFNCPLHEEENPSAALYKTDNGVIGMKDFHRSGAFYTLPQLY
                     YEYKTGNDNRLRGASSLIWWIRLLKEVGFVEPPRIMPPKPLEKLSKNQKILYKAFINL
                     LELQQVYDPNQKAAPFSHRFAASWSGLKYESVRSAKRGLSKKKYIKKVEPGDRKTRKA
                     GKWALVRD"
BASE COUNT         1047 a          345 c          512 g          733 t
ORIGIN      
        1 aaaattcagc gaaaaaacat tatcactttt taagcaggta tttggccaca aaattaaatc
       61 ttttgagctg caactattaa acaaatatga tctacctgat gaagttattc gcaaaggaat
      121 agaactatca ggcacacaca atgcccagag ccttcaatat ttgcttgata tactgaaaga
      181 atggtcaagc aggggtataa ctacccttga aggaatagaa cgctatataa ggcaatttaa
      241 ggggcaagaa acagacgagg atttggaagc taaaggttac agataagaat attatttgaa
      301 agcaaacata atccctgtct atatagggtt tatagaaaaa aatgcacttc tagaacactt
      361 ttagcgaact attggaagac acgctgaaat taaagaaata tgaaaaaaaa caaaatatta
      421 aagtagttat tatccttgaa atgacaaggc ttttataaaa agacatataa catactaata
      481 acagttgaca aacggtgtta tatttggtat aattattata gtgagaagta tataaattaa
      541 taatatttag caactcggta atttaatacc gggttatttt tatgtctaat tttaattaat
      601 cactcggggt aaaaaccgag tttttttaat gcctaaaatt aaataatgtt tactaattaa
      661 ctcggtgaaa ttccgggtta tttttatgcc caattttaaa ataaggaggt gaaaatatga
      721 aatttgaaaa tatagagatc accaaaagac ttaataaaac gaaagttgat acgcaaggga
      781 aggtctatat tcctgccaag attagagaat tatgtggaat tgaaaaaaat tctgaagtta
      841 atatccaggt ggacaaagac agtaaagcag tattgataag atagggggat aatatgaagt
      901 ggggagaagt aaaaaaaggt caactagtaa agataggtcc ttcaaacttt gtaggatata
      961 tttataaact agatcataaa gaaaaaatgg tcaaggtgga atggactgga agttggtgtg
     1021 attggaataa gcctgaaaaa ttagatttaa ttgaaatatc agattttaat catttcatta
     1081 aaggttggca aaatgctaga aaagattatt aaaataaaaa aactacctgc cgttgaacag
     1141 gtagcaatca atgtaaacct actattaaaa ccaacttctt gcgaaaggag gttttacaat
     1201 tcacattata gcagagaatg gtcagcaaat caataacgac aaaaaaacag ctgctttgga
     1261 aatgagccgt aaaggttatc atttactacc tttaaaacca ggtgataaga taccatattt
     1321 cgatttattg ccaggaaatt caacagatat atttaaagaa aatcaagcta aaagtagttt
     1381 agttgaaagt tggattaaac aagatccgca ggttaattta ggagtattcg ctggaactga
     1441 aatatcaaaa aattacagat tagttatagt tgactttgat aaagaaatga atttcggact
     1501 accaattaca ccaatggtca agactaaaag aggttatcac gcatatttta agtgtagaac
     1561 tgacaggctg ccagaagccc acaaaacgcc cagaggggaa attaaaacat cggggtatgt
     1621 agtagctcca ccaagcaaac acccgtcagg aatggtctat aaatgggctg attacttaag
     1681 tttcattgat gtaggcctgg cagaatttca ccaacgaagc gaacagataa tatcattttt
     1741 agaaggaacg aattccgacc gtttttatta taatcagaga gatgaagata agttaaacac
     1801 tacaattaat attaatagta gtgttaagaa tagagatcct ctatctatta gtaaagatat
     1861 aaaagaaaat agaaagaagt tagagcagtt aagtaggtct gaagaagtgg ctttatcagt
     1921 aatgagagaa gtgtttgaaa tagaggtcgg aagggtagga aggactttta attgcccgtt
     1981 acacgaagaa gaaaatcctt cagcagcctt atataaaact gacaacggtg ttataggaat
     2041 gaaggacttc cacagatcag gggcttttta cacgctgcca cagttatatt atgaatacaa
     2101 aacagggaat gacaacagat taagaggagc ttcttcttta atttggtgga taaggttatt
     2161 aaaagaagtt ggatttgtag agccgcccag aattatgcca ccaaaaccat tagaaaaatt
     2221 atctaaaaat caaaagatat tatacaaagc gtttatcaac ttattagaat tgcaacaagt
     2281 atatgatcca aaccaaaaag cagctccttt tagtcatagg tttgcagcta gttggagcgg
     2341 tttgaagtat gaaagtgtaa gaagtgccaa gcgtggacta tctaaaaaga aatatataaa
     2401 aaaagttgag cctggtgatc gtaaaactag aaaagcaggt aaatgggctt tagttagaga
     2461 ttaaaaataa ttaaaaggtg gtgtattatg caagagttac tattatcagc aatattaata
     2521 agcggtttat gtgtagtcag ctttaaagta ggcggaatta tatcaacaaa aataaataaa
     2581 taaagatatg tattcgtaga tactagttat aaatatgtag tcgcagaaaa ggtatat
//
LOCUS       DATGCJ010000014         7080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_224982, whole genome shotgun sequence.
ACCESSION   DATGCJ010000014 DATGCJ010000000
VERSION     DATGCJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224982"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VKN64_00595"
     CDS             complement(<1..1249)
                     /locus_tag="VKN64_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMA58759.1"
                     /translation="MSPLFIVLGIGLLIPIIFCFLYQEPHIYYQSFYIPSLISFGLGI
                     LFSFLKTEDINLSLTNSMLIVGLSWIFFSIIGGIPFIIGLDKSFIDAFFEAVSGFTTT
                     GITVFQNLSTIPHSILIWRSLIQWFGGLGILTFFLFITFRSEGELWQLFTAESHKIDT
                     SRPVPNVFRSIKILWLIYGLFTVFQIILLIAVQVTPFDALLHALTTLSTGGFSNYDAS
                     IAHYAISGHPYYKAIEYIMIFFMLLGGINFLIHYKVLKGDIKEVFTNFEMKYFWGIIG
                     TVMLLILTGKLFNQSLNFANLEMVFRKSLFQVVSVMTTTGYGTESLTSTFFPAIARQL
                     FLFLMLIGGCVGSTAGGIKVIRFAVLNKLFGREIKKFYLPKRAVMPLRIEKKLIPRDE
                     IYKISALFFFWLLIILVGGIITAL"
     gene            1431..2363
                     /locus_tag="VKN64_00600"
     CDS             1431..2363
                     /locus_tag="VKN64_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMA58760.1"
                     /translation="MSRNLTYKKAVDSFKKYLKVDRAYSPLTIKEYEHDLKLLYRYLT
                     NEFDFEEDFKITDITKYEVSEFLSDIVLSYDNSAVTRNRKLYAIRSFFKFLQKREYLD
                     KNPTMTIESSKTRTTAEPIYMKIKEAKQYIKTIKEADLLNKKRDLAIVKLFLYAGLRV
                     SELVELNLDDINYEDKSIKFYGKGNKERYVPLHTDTIKAIKNYLPERNKIEPNDKDAE
                     KAIFLSRRGNRISARSIQMFVKKYAKKAGIKNASKITPHKLRHTFATILYHNTKDIKV
                     LQDLLGHSNISTTQIYTHTDQKQKKEAIEELPDF"
     gene            complement(2373..2448)
                     /locus_tag="VKN64_00605"
     tRNA            complement(2373..2448)
                     /locus_tag="VKN64_00605"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2413..2415),aa:Phe,seq:gaa)
     gene            complement(2474..2550)
                     /locus_tag="VKN64_00610"
     tRNA            complement(2474..2550)
                     /locus_tag="VKN64_00610"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2514..2516),aa:Met,seq:cat)
     gene            complement(2558..2633)
                     /locus_tag="VKN64_00615"
     tRNA            complement(2558..2633)
                     /locus_tag="VKN64_00615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2598..2600),aa:Lys,seq:ttt)
     gene            2842..3909
                     /locus_tag="VKN64_00620"
     CDS             2842..3909
                     /locus_tag="VKN64_00620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMA58761.1"
                     /translation="MNFNFLKDKYLSFDDYIDFKYFLAQMILYAVFAVMIAEIIISFF
                     IYNKPVTDLIILRIISIIFYLFLIFFMKKDLKRMSLITKILFVFLIIESVFFSFVGYG
                     DYLIVWIIASQLVFGAFSTSKELLKALTGYYFALYMGISIYTGQMEVHLHRYIYFTFI
                     ITVNYYFYLGIEKYFAQHEVINNMEEAIEDLRKNSSFSLKDALTNTYNRKSLYSILKK
                     EIALVKQNNNVSSLAFIDLDNFKEINDKYGHQHGDQILKEISTIFLNVIREDDYICRF
                     GGDEFIFILSECKKETAVEIISRIKQDINDHLNRENIHLDFSYGIKEIRPDIEMSSKE
                     IIEKADNLMYKDKNNKKQNIS"
     gene            complement(3988..4611)
                     /locus_tag="VKN64_00625"
     CDS             complement(3988..4611)
                     /locus_tag="VKN64_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58762.1"
                     /translation="MSNTNKGKTFKANTIEQTNLLTDPFGLTVLSTLEADKYITAEEI
                     AEQSGEELELIEEYLNLFIKEDMIQSKKENDKKKYSKKAEYYSFSPELLSMIPDQIQD
                     HIIFGLLHALQGDYYDLLKLAKEHDNLDQALEKAGYPEPKKSLDLVMGRIYIKEDDIE
                     ELEAHIQELFDKYSNIPEGEEEEDYLAFDLNFFMKPYIASFMKKMKE"
     gene            complement(4731..5336)
                     /gene="prxU"
                     /locus_tag="VKN64_00630"
     CDS             complement(4731..5336)
                     /gene="prxU"
                     /locus_tag="VKN64_00630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850782.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5196..5198),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HMA58763.1"
                     /translation="MLKVGAKAPDFEGSAFHNGDFKKVKLSDYEGQWRIVCFYPGDFT
                     YVUPTELSAVAVKYDEIKDLGAEVIAVSTNSIQSHKIWNEVELSKMVEGGIPYPMVSD
                     SAGKIGRKYGVYDEDAGIDVRGRFLIDPDGVLQAMEVLTPPVGRNVEELIRQLQAFKH
                     VRETGEATPSGWRPGKPTLKPGKELVGKVWKEWQPDNAFKE"
     gene            5587..6726
                     /locus_tag="VKN64_00635"
     CDS             5587..6726
                     /locus_tag="VKN64_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMA58764.1"
                     /translation="MKKLKAFPKGLLVIVMAAVLIGTLSTGLYAQSNDTPSPTQLSNQ
                     FSQIAEKVDPGVVKVTSKIKVSDGQLPYYYDEDFYEYFFGEQLPEEQPKYQEGYGSGF
                     IVSQDGYIVTNEHVVHNATEINVAINGFEEPVPAELVWAEYDLDLAILKVDVNKELNP
                     IPLGDSKALKPGDWVIAIGNPFGLSHTVTTGVVSALGRPIQIPSSSGPARTYKNLIQT
                     DAAINPGNSGGPLLNINGEVIGINTAVSQRGQGIGFAIPINEVKGYIDDLKTKGEIKR
                     PWLGIVYGPVTDKVQSYFELENKNGILVHRVMEDSPAAKAGIKQYDIIKEIDKQPIKE
                     LEDVSEIINNTEIGDTIMIKVVREGTSEILFAEIEKKPADISSKF"
     gene            6869..>7080
                     /locus_tag="VKN64_00640"
     CDS             6869..>7080
                     /locus_tag="VKN64_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58765.1"
                     /translation="MVSKKFITLSLVFILLFSIPMGVLAQDSNTTQQEQQTEVAAVIN
                     GNEVTMKQLNQFAGTNQLVMSIYRQN"
BASE COUNT         2535 a         1165 c         1070 g         2310 t
ORIGIN      
        1 ataaagctgt aattattccc cctactaata ttatcaataa ccagaagaaa aacagggccg
       61 aaatcttata tatctcatct ctgggtatta atttcttttc tatccttaaa ggcattactg
      121 ctctttttgg tagataaaac ttcttaattt ctcttccaaa caatttattt aaaactgcaa
      181 atcttataac cttgatacct ccggctgtag agcccacaca acccccgatc aacatcagaa
      241 agagaaataa ctgccgggct atcgcaggaa aaaaggttga agttaaagat tcagtcccat
      301 agccggtcgt agtcataaca gaaactacct ggaaaagact ttttctaaag accatctcca
      361 gattagcgaa attaagggat tggttgaaca gtttgcccgt taaaataagt aacatcactg
      421 tgccaataat tccccaaaaa tatttcatct caaagtttgt aaatacttct tttatatctc
      481 ctttaagcac tttatagtgg attaaaaagt taataccacc caataacata aagaatatca
      541 ttatatactc aatcgcttta taatagggat gcccacttat tgcataatgg gcgatactag
      601 cgtcataatt tgaaaacccg cccgtcgata aagtagttaa agcgtgtaac aaggcatcaa
      661 agggtgttac ctgaactgcg attaaaagta taatttgaaa tacggtaaac agtccatata
      721 ttaaccagag tatttttatt gacctaaata cattgggaac cggacgagaa gtatctattt
      781 tgtgagattc ggcagtaaac agctgccaca gctctccttc acttctaaat gtgataaaca
      841 ggaaaaaggt taaaatacca agtccgccga accactggat tagactacgc cagattaaaa
      901 ttgaatgtgg tatagtagaa agattttgaa agacagtaat tcctgttgtc gtaaaaccac
      961 taactgcttc aaagaatgca tctatgaaac ttttatccag gcctatgata aagggtatac
     1021 caccaattat ggagaaaaat atccaggaaa gtcctacaat taacatacta tttgttaatg
     1081 ataagttaat atcttccgtt tttaaaaagg aaaataagat tcctagtcca aaagatatta
     1141 gggaaggtat ataaaaagat tggtaataaa tatgtggttc ctgataaaga aaacaaaata
     1201 ttattggaat tagaagccca atacctaata caataaataa aggactcatt atgtataata
     1261 tggtctttat cttcctggaa acaatgatgg taaatcaccc ctacatttct ttttaataat
     1321 tatataataa attctaaata aagtaaacgt acttaaatta agcaggatta ataaaaacaa
     1381 tattgaaatt atataattaa taacccttat gattaaggag cctgatactt atgagtagaa
     1441 accttacata caagaaagca gtcgatagtt ttaagaaata tcttaaagta gatagggcct
     1501 attcccccct aactattaaa gaatatgaac atgatttaaa acttttatat agatatctta
     1561 ctaatgaatt tgattttgaa gaagatttca agatcacaga catcactaaa tatgaggtct
     1621 cggaatttct ttccgatatc gtattaagtt atgataatag tgcagtaacc cgaaatcgta
     1681 agctctatgc aatccgttct ttctttaaat tcttacaaaa aagagagtat ttagataaaa
     1741 atcccaccat gacaattgaa tcttccaaaa ctagaaccac cgctgaacct atttatatga
     1801 agatcaaaga agccaaacaa tatataaaaa cgattaaaga agctgactta ttaaataaaa
     1861 aacgtgattt agctattgtt aaactctttt tatatgcagg tttaagggtt tcggaattgg
     1921 tcgaattaaa tttagatgat attaattatg aagacaaatc gattaaattt tatgggaaag
     1981 ggaataaaga aaggtatgtt cctcttcata ctgatactat aaaagctata aaaaattact
     2041 taccggaaag aaataaaatt gagcctaatg ataaagatgc tgaaaaagcc atctttttat
     2101 ccaggcgggg taatagaatt agcgcccgtt ctattcaaat gtttgttaaa aaatatgcca
     2161 aaaaagcggg aataaaaaat gcaagtaaga ttactcccca caaactaagg catacttttg
     2221 caacaatttt ataccacaat acgaaggata ttaaagtcct gcaggatcta ttgggacatt
     2281 ctaatatttc tacaactcaa atttataccc acaccgacca aaaacaaaaa aaggaagcta
     2341 tcgaagagct tccagacttt tagataaaaa aatggtggtg agagccagag tcgaactggc
     2401 gacacgagga ttttcagtcc actgctctac cacctgagct acctcaccac acaaaatttt
     2461 ttaaatatta aattggttgc gggggccgga ttcgaaccga caaccttcag gttatgagcc
     2521 tgatgagcta ccagattgct ctaccccgcg ttaaaaatgg tgagccatgg aggaatcgaa
     2581 cctccgacaa cctgattaaa agtcaggtgc tctaccgact gagctaatgg cccctaatca
     2641 acggttaata tattaacatc ttttccctat aaagtcaaga gtttcgacct taattccact
     2701 ttaatttatt aaattttttt aaaaaaatat aatgataaaa ttttattttt ttctgattaa
     2761 aagtgagcag gattcataat tataatattg aataacttta ttatagaata attatacaat
     2821 ttataacagg agttgtaata aatgaatttt aattttttga aagacaaata tttaagtttt
     2881 gatgattata tagattttaa atattttctt gcacagatga tcttatacgc cgtctttgcc
     2941 gtaatgattg cagaaataat aatctctttt tttatttata ataaaccggt tactgattta
     3001 ataattttac gtataatatc catcattttt tatctttttt taatattttt tatgaaaaaa
     3061 gatttaaaaa gaatgtcttt aataacaaaa atactatttg tctttcttat aattgagagt
     3121 gttttctttt catttgttgg ttatggagac tatctaattg tttggataat agcttctcaa
     3181 ttagttttcg gagcattctc aaccagtaaa gagcttttaa aagcgttaac tggttattat
     3241 ttcgcactct atatgggtat ttctatatat acaggccaaa tggaagtcca tcttcacaga
     3301 tatatatatt ttacttttat tataactgta aattattatt tttatttagg aatagaaaaa
     3361 tactttgcac aacatgaagt aattaataat atggaagaag caattgaaga cttaagaaaa
     3421 aactcttctt tttctttaaa agatgcctta actaatacct ataatagaaa gtcactttat
     3481 tctattctca aaaaagaaat cgccttagta aaacaaaata ataatgtatc ttctctcgca
     3541 tttattgacc tcgataattt caaggaaata aatgataagt atggccacca acacggagat
     3601 caaatattaa aagaaatcag tactattttc ttgaatgtca tcagggaaga tgactatatc
     3661 tgtcgctttg gtggagatga atttatcttt attctctctg aatgtaaaaa agaaaccgct
     3721 gtcgaaataa tctcgaggat aaaacaggat attaatgacc atttaaacag agaaaatatc
     3781 catttagatt tcagttatgg gattaaagaa attcgacctg atattgaaat gagttctaaa
     3841 gaaattattg aaaaagctga taatctaatg tataaagata aaaataataa gaagcagaat
     3901 atctcataag aaaatttaaa actgttagta aaactcaaaa aagtcctcaa atgaggactt
     3961 tttttattat tttattatat ttatctttta ctctttcatt tttttcatga aactagctat
     4021 gtaaggtttc ataaaaaagt ttaaatcaaa agctaagtaa tcttcttcct cttctccttc
     4081 tggtatattt gaatacttgt caaacaattc ttggatgtgt gcttccagtt cttcaatatc
     4141 atcttcttta atataaattc tacccattac taaatccaaa ctcttctttg gttcaggata
     4201 accggctttt tccagggcct gatccaaatt atcatgttct ttagctaatt ttaataaatc
     4261 ataataatct ccctgaagag catgtaataa accaaaaata atatgatctt gaatttggtc
     4321 aggaatcata gataataatt ccggtgagaa actatagtat tcagcctttt tagaatattt
     4381 ttttttatca ttttcctttt tagattggat catatcttct tttataaata agtttaaata
     4441 ctcttcaatt aattccaatt cttctcccga ctgttcagca atttcctctg ctgttatgta
     4501 tttgtctgcc tccaaggtgg ataaaacagt taaaccaaaa ggatctgtta atagattggt
     4561 ctgttcaata gtattagctt taaatgtctt tcctttatta gtattagaca tgttgactta
     4621 cctccattat tgcttattat attatcaatt ctttttaata tgatattttc ctgcttataa
     4681 gataaaataa agggccctga attcgggccc ttttttataa cttattttat ttattctttg
     4741 aaggcattat ctggctgcca ttctttccac acttttccaa ctaattcttt gccgggtttt
     4801 aaagttggct taccgggtct ccagcctgaa ggtgttgcct ctcctgtttc tctaacgtgt
     4861 ttaaatgctt gtaattgtct tataagttct tctacatttc ttcctaccgg tggtgttaag
     4921 acttccatag cctgtaatac tccgtcagga tcaattaaaa atcttcctct tacatctatt
     4981 ccagcatctt catcataaac tccgtatttt ctaccaatct ttccagcaga atcggaaacc
     5041 attggataag gaattccacc ttctaccatt tttgacagtt ctacttcatt ccaaatttta
     5101 tgtgattgaa tactgttagt acttactgca attacttctg cccctaaatc cttaatttca
     5161 tcatacttga cggcaactgc cgacagttca gtaggtcaga cgtaagtgaa atcgcctgga
     5221 tagaaacaaa caattctcca ttgtccctca taatcagata actttacttt cttaaaatct
     5281 ccattgtgaa aggcacttcc ttcaaaatct ggtgccttag cgccaacttt taacatattt
     5341 ttccctcctc aaatttagtt taaaaatgtg ttattttaaa tatagcataa acaataataa
     5401 ttatcaataa atatatctaa aaaattaatt atacagcatt tccatttttt atatatagaa
     5461 taatacaata tttttatact tttctcatat ttctcttaca tttgccctgt atattattta
     5521 ttagaaagca aaatggaaat ataactaaat attacagctc tgaaaattaa aggagggaaa
     5581 ttattcatga aaaaacttaa agcctttcca aaaggattat tggtaatagt aatggcagct
     5641 gtattaatcg gtaccctttc taccggttta tatgctcaat ccaatgatac tcccagtcca
     5701 actcaactga gtaaccaatt tagtcaaata gctgaaaaag ttgacccggg agtagtaaag
     5761 gttacttcta aaataaaagt tagtgatgga cagcttcctt attattacga tgaagatttt
     5821 tatgaatatt tctttgggga acaattacca gaagaacaac caaaatatca agaaggttat
     5881 ggtagtggat ttattgtttc tcaagatggt tatatcgtaa caaatgaaca tgttgtgcat
     5941 aatgccactg aaattaatgt agccataaat gggtttgaag aacccgtacc ggctgaactt
     6001 gtctgggccg aatatgacct ggatttagcc attttaaagg tagacgttaa taaagagtta
     6061 aatcccattc cattaggcga ttcaaaagct ctaaaacccg gtgattgggt tatagctata
     6121 ggaaatccct tcggactaag tcacactgtt acaacaggtg ttgtaagcgc tctgggacgt
     6181 cctattcaaa taccctctag tagtggtccg gcgagaacct ataaaaattt aatccaaacc
     6241 gatgccgcaa ttaatcctgg taatagtggt ggaccattac tcaatataaa tggtgaggta
     6301 ataggtatca acaccgctgt tagtcaacga ggacagggaa ttggttttgc aattccaatt
     6361 aatgaagtaa aaggatatat agacgatctg aaaacaaaag gagaaattaa aagaccttgg
     6421 ttaggtatcg tatatggtcc tgtaacagat aaagtacaaa gttactttga actagaaaat
     6481 aaaaatggta tattggtcca cagggttatg gaagatagcc cagccgctaa agcaggcata
     6541 aagcagtatg atataataaa agaaattgat aagcaaccaa tcaaagaact agaggatgtt
     6601 tctgaaatta taaataatac tgagattggt gatactataa tgattaaagt tgtacgagaa
     6661 ggtacttcag aaatactatt cgctgaaata gaaaagaaac cggccgatat ttctagtaaa
     6721 ttctaattga caaatcataa ccaaagatta ataacaaaaa ataaaatggg ttctaaaatt
     6781 agcatataat tttggaaccc attctttcat atatataata aaaataatat caaattttaa
     6841 aacacaaaat aaaggagtga aattaataat ggtttcaaaa aaatttatta cactttcatt
     6901 agttttcatc ttactcttct ctattccaat gggggtctta gctcaagaca gtaatacaac
     6961 tcagcaagaa caacaaacag aggttgctgc agtaatcaat ggtaatgaag taacaatgaa
     7021 acagttgaat caatttgcag gaaccaatca gcttgttatg agtatttata ggcaaaacaa
//
LOCUS       DATGCJ010000015         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_91294, whole genome shotgun sequence.
ACCESSION   DATGCJ010000015 DATGCJ010000000
VERSION     DATGCJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91294"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VKN64_00645"
     CDS             <1..291
                     /locus_tag="VKN64_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HMA58766.1"
                     /translation="YIGKIISRRMKNQGMSWKRLGAEAMVKILMLSHNEILKTRLNDK
                     SYKIKNPIKKLKYRERVFKRNWNDWLQASRPVIYGPCSGKNWVKGIKSIATI"
     gene            428..859
                     /locus_tag="VKN64_00650"
     CDS             428..859
                     /locus_tag="VKN64_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMA58767.1"
                     /translation="MKDSSIGKYLSITHRAHTSLVDKKFKEKFNLSHGQVFILINIYR
                     DEGIYQHRLCEEYNLDKSGVGRILKRLEEKNLVKRKSDPDDKRRKLIFLTGKAKEMKS
                     EFKKLLKEIEEQMRQGLTEEEIKTVTNILKKIYNNLDKSNV"
     gene            878..2263
                     /locus_tag="VKN64_00655"
     CDS             878..2263
                     /locus_tag="VKN64_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMA58768.1"
                     /translation="MALETKSSRMGTQPIGKLLFKLSAPAIVGMLIQALYNIVDSIYV
                     GRLSTEALSALSVSFPVQMFLIAIGVGTGVGTSSLISRLLGRGNKKRANNVAEHVFFI
                     AILYGIFVGLIGIFFSSNIIRLFTDDPVLIDLGYQYINVILTGSIAIFIPATFNYILR
                     GEGNTFVPMLTMLIGAILNMIIDPFLIFGIGPFPQLGVAGAAYATVFSRIVGGIFIIF
                     VLFSDKNELTLKLEDFDFDFGIIKEIYQVGLPAMVNRLLFSTAMVFINLILGAFNSTA
                     IAVMGLIFRMQSFFLMMVFGLTQGYLPLVGYNFGHNNPDRMKRTIIIGNAAALSFGAL
                     GFLVFQLFPETIITLFNSDPKLLGIGIPALRRVSMSYFFMVLNITGVATFQAIGRGMP
                     SFVITFLRQAIFLVPGMYLLGQFFGLEATWLAFPIGESISFAIMATWLYGTIKNSMYE
                     MKKQKPQKNTV"
     gene            complement(2355..3695)
                     /locus_tag="VKN64_00660"
     CDS             complement(2355..3695)
                     /locus_tag="VKN64_00660"
                     /inference="COORDINATES: protein motif:HMM:NF018913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58769.1"
                     /translation="MNTENKPLSYHILIKFFLPLAVFPVLVAMSHTVINSALARMPLP
                     ELSIAVFTVVKSVSNIFNAPTIMTRQLFTSIIEDEKSYFLSRNFAFILMGVLFVMIMS
                     LGITPLGDFVYKNIIGLQSQKEINLATSAMMVTAFLPIVVVLRNAYQGIATGLKKTLL
                     IVPGVVLRVIGMIFFLWWVVKTNSINGVIAGCLAYVLGIGLEGVFILGGLQYKMGSIL
                     EAVKKMPASDFESESLDYYKIFKFFLPLAFTIIITMFIQPVIQSGLARSSSPVRSLAA
                     YGVSWSLITMLAGPLRMLHQISLVYHNSNENMNKIKIFNLSLGILTSVIIVIFALSPI
                     GYFVLHNLIAVSHEITLLAQKVILAFAAFPLARAFRETYWGMMMKKRSTPVIAKAKGL
                     NLFAVILSLVVLLGFINVYFNIEPAFLGAVVFTIGEFLESFIIKRNSKNIESTA"
     gene            complement(3743..4324)
                     /locus_tag="VKN64_00665"
     CDS             complement(3743..4324)
                     /locus_tag="VKN64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HMA58770.1"
                     /translation="MSKVLIPLAAGFEEIEAVTNIDVLRRASIEVVTVSLASLDVMGD
                     HDIPIIADKRIDQVNIDEFDAIVLPGGMPGAGNLRDNAKVLEYVRKIDEKGGLVAAIC
                     AAPMVLEAADLLEGKNATSYPGFAKEMQSCNYKEDRVVIDGNIITARGPGVAMEFALT
                     IVEYLRDKEYADDLRDSMIVEKPAKKKKNKNNK"
     gene            complement(4341..4940)
                     /locus_tag="VKN64_00670"
     CDS             complement(4341..4940)
                     /locus_tag="VKN64_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMA58771.1"
                     /translation="MVKNVVFDLGNVLLDFNSDEIIADYVKDEKLHQQISENIFNSKE
                     WILLDRGEITAKEATNRFIKRIPDKEKLVREIMDNWKHYLTPIQVNVEVLNNIAQKPI
                     NLYVLSNFHKEAFEEVYEKYDFFNHFDGMIISYREKTVKPERKIYEKLINRYNLKPDT
                     TLFIDDSERNIEAAKKLGFKTIHFNDNMKLSNSITDYIR"
     gene            complement(4933..5862)
                     /locus_tag="VKN64_00675"
     CDS             complement(4933..5862)
                     /locus_tag="VKN64_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA58772.1"
                     /translation="MSILVTGGAGFIGSNMVDALIDRGEEVVIVDNLSTGFKENVNEK
                     AQFYNIDLTDRKLFDIFDNYAIRKVIHLGAQIDVTRSVHDPIYDSHVNIQGSINLFQA
                     CVKHDIEKLVYASSAAVYGEPNYLPINEKHPVKAMSPYGVSKHTPEHYLKYFNNAYDL
                     NYTVLRYSNAYGPRQSPAGEGGVIAIFIDRMLEDKRPIIYGDGKQTRDFIHVYDIIEA
                     NLKALDTASEKTLNVSTRSETTINKLVKLINEELAKKLEPIYKERREGDIRHSILDNS
                     RALDYLDWEPQYEIEDGIKQTVNYYLKRSQNNG"
     gene            complement(5910..6125)
                     /locus_tag="VKN64_00680"
     CDS             complement(5910..6125)
                     /locus_tag="VKN64_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58773.1"
                     /translation="MNIKNIGLVVLILAAVLLLGNGIYHLIMNLLIDTDLSLLVKIGI
                     FGLIIGLIILIIGLIIERIKDKKDEQF"
BASE COUNT         2013 a         1154 c          989 g         2075 t
ORIGIN      
        1 tatataggca agattatatc taggcgaatg aaaaatcagg gtatgagttg gaaaaggctg
       61 ggagccgaag cgatggttaa gattttaatg ttgagtcaca acgaaatatt aaaaacaaga
      121 ttaaatgaca aatcttacaa gataaagaat cctataaaaa aattaaaata tcgggaaaga
      181 gtctttaaaa ggaactggaa tgattggctg caagcaagta ggccagttat atatggacct
      241 tgtagcggca aaaactgggt taaaggcata aagtcaatag ctactatata aataaaatat
      301 attgaggtct ttatctttcc caccttttct ttacgcaaac aaaatcttat atttagttga
      361 catatcaact tatttgtcct ataatacttg ttgacatatc aactaaatag ttagaggtgt
      421 tgtattaatg aaggacagtt ctatcggtaa atatctaagt atcactcata gagcccatac
      481 atctttagtc gataaaaagt ttaaggagaa gttcaatctt agtcatggtc aggtatttat
      541 cctgattaat atataccggg atgaaggaat ttatcagcat agattatgtg aagaatataa
      601 tctggataag tctggtgtgg gaagaatttt aaagaggtta gaagaaaaga atttagtgaa
      661 acgcaaatca gatccggatg acaaaaggag aaaattaata tttcttaccg gcaaagcaaa
      721 agaaatgaaa tctgagttta aaaaactatt aaaggaaatc gaagagcaaa tgagacaggg
      781 tttaactgaa gaagaaatta agaccgtaac aaatattctc aaaaaaatat ataataatct
      841 agataaatca aatgtttaat ctttttggag ggatagaatg gctttagaaa caaaatcaag
      901 ccgaatgggt acacaaccaa tcggaaaatt actgtttaaa ctatctgcac ccgcaattgt
      961 ggggatgctc attcaggctt tatataatat tgtagatagt atctatgtag gacgtttaag
     1021 tacagaagca ttatcagctt tatctgtatc cttcccggtt cagatgtttt taattgcaat
     1081 cggggttggt acaggagttg gcaccagctc actaatttcc agacttttag gtagaggtaa
     1141 taaaaaacgg gccaataatg tagcagaaca tgttttcttt attgcaatct tatacgggat
     1201 ctttgtagga cttattggaa tcttcttctc atctaatata attagattat ttacagatga
     1261 tcccgtacta attgacttgg gataccagta tataaatgtt atcttaaccg gttcaatagc
     1321 catatttata ccggccacct tcaactatat attaagaggt gaaggcaata cttttgttcc
     1381 gatgctgacg atgttaattg gggccatctt aaatatgata atagacccct ttttaatctt
     1441 tggtattgga ccatttccac agttaggggt agcaggggct gcttatgcaa ctgttttctc
     1501 tagaattgtg ggtggtatct tcatcatctt tgtactgttt agtgataaaa atgaactgac
     1561 cttaaaacta gaagactttg actttgattt tgggatcatt aaagaaatat atcaggttgg
     1621 tttacctgca atggtcaatc ggctgctctt tagtaccgcc atggtcttta ttaacttaat
     1681 tctaggagcc tttaattcaa ccgcaattgc tgttatgggt ttaatcttta gaatgcagtc
     1741 ctttttcctg atgatggtct tcggcttaac acagggttat ctacccctgg tcggatataa
     1801 ttttgggcat aataatccgg accggatgaa aagaacaata ataattggta atgcagccgc
     1861 cttatcattt ggagcattag gctttttagt attccaacta tttccagaaa caattatcac
     1921 cttatttaat agcgacccca aattactggg aattgggata cctgccctaa gaagggtaag
     1981 catgtcttac ttctttatgg tcttaaatat tacaggagtg gctaccttcc aagcgatcgg
     2041 tagaggtatg cccagttttg taatcacatt tttaagacag gctatatttt tagttcccgg
     2101 catgtatctt ttaggccagt tctttggctt agaagctacc tggcttgcct tcccaattgg
     2161 ggagtcaatt agttttgcca taatggcaac ctggctttat ggaactatta aaaattccat
     2221 gtatgaaatg aaaaaacaaa aaccgcaaaa aaatacagtt taaaaaaata ttaatagtta
     2281 ttgaaataca aaattcccat ccatctggat gggaattaac tatttatatt taatttaaat
     2341 tatataatat ttacctaagc agtactttca atatttttac tattcctttt aataataaac
     2401 gattctaaaa actctccaat cgtaaataca actgccccca agaatgccgg ttcaatattg
     2461 aaatatacat ttataaaacc taataatact actagagata aaatcacagc aaatagattt
     2521 aagcccttgg ctttagcaat aactggagtt gatctttttt tcatcatcat tccccagtag
     2581 gtttctctaa aggctcttgc taagggaaaa gccgcaaaag caagtattac cttttgggct
     2641 aaaagagtaa tctcatggga gacagcaatt aaattatgta aaacaaaata tccaatcggt
     2701 gataaagcaa atatgactat tattacagag gttagtattc ctaaagaaag attaaatatc
     2761 ttaatcttat tcatattttc attactatta tgataaacaa gtgatatttg atgcagcatt
     2821 cttaacggcc cggctaacat cgtaataagc gaccaggaga ctccataagc agccagagat
     2881 ctcacgggtg aactacttct ggccaaacca ctttgaatta caggctgaat aaacatcgta
     2941 atgataatag taaaggccag gggtaaaaag aatttaaata tcttatagta atcaagactt
     3001 tcactttcaa aatcagaggc cggcatcttc tttacagctt ctaaaatact gcccatctta
     3061 tattgtaaac cacctaaaat aaagactccc tctaaaccga tgcccagtac ataagccaaa
     3121 caacctgcga taactccatt gatactgttg gtctttacta cccaccataa aaagaagatc
     3181 atgccgataa ctcttaatac cacaccaggt acaattaaaa gtgtcttttt aagtccagtc
     3241 gcaatcccct gataggcatt tctcaaaacc actactatag gtaaaaaagc tgttaccatc
     3301 atggctgagg tagccaggtt aatctctttt tgggactgta aacctataat atttttataa
     3361 acaaaatcac ccaggggagt tatacccaaa gacataatca taacaaacaa gacacccatc
     3421 aatatgaaag caaaattacg ggaaagaaaa taacttttct catcttcaat gatagaggta
     3481 aaaagttgtc tggtcattat ggtaggtgca ttgaagatat tactaacact ctttacaacc
     3541 gtaaaaacag cgatacttaa ctccggtaag ggcatccttg ccaaagcact gttaataact
     3601 gtatgtgaca ttgctactaa aactgggaaa acggccaggg gtaaaaagaa ctttattaaa
     3661 atgtggtaac ttaatggttt attttcagta ttcaagcttt ccctgacctc cccgttaatt
     3721 gaatattata ttacttactt aattatttat tattcttatt tttctttttc ttggctggct
     3781 tttcaactat catggaatct ctaagatcat cagcatattc cttatctctt agatattcta
     3841 caattgttaa ggcaaattcc atagctactc ccggtccgcg ggcagttatg atattaccat
     3901 ctattacaac cctatcttct ttataattac aggactgcat ctctttagca aaaccaggat
     3961 aactggttgc atttttacct tctagtaaat cagccgcttc taagaccatc ggtgcagcac
     4021 agatggcagc aactaggccg cctttttcat ctatttttct aacatattct aatactttcg
     4081 cattgtctct taaattacct gctcccggca ttccgcccgg cagcacgatg gcatcaaatt
     4141 catcgatatt tacctgatct attcttttat ctgcaataat tggtatatca tgatcaccca
     4201 taacatcgag tgaagcaagg ctaactgtta caacctcgat actggctctc cttaaaacat
     4261 cgatattagt gacagcttca atctcttcaa aacctgctgc taaaggtatt aatactttac
     4321 tcatctatat ccactccttc ttatctaata tagtcggtta ttgaattact caacttcata
     4381 ttatcattaa aatggatagt tttaaatccc aattttttag ctgcttcaat atttctttca
     4441 ctgtcatcaa taaataatgt tgtatctggc tttaaattat atctattaat taacttttca
     4501 tagatttttc tttccggttt gacggtcttt tcccggtaag atattatcat gccgtcaaaa
     4561 tggttgaaaa aatcatattt ttcataaact tcttcaaagg cttctttatg gaagttagaa
     4621 agcacgtata aatttattgg tttttgagca atattattta aaacctcaac attgacctga
     4681 ataggagtta aatagtgttt ccagttatcc atgatttctc ttactaactt ttctttatca
     4741 ggtatccttt ttataaatct attagtagcc tctttcgctg taatttctcc cctatcaagc
     4801 aggatccact ctttactgtt aaatatattt tcagaaatct gttgatgaag tttttcatct
     4861 tttacgtaat cagctattat ttcatcagaa ttaaagtcta atagtacatt acctaaatca
     4921 aagactacat ttttaaccat tgttttgact ccttttaaga taatagttga cagtctgttt
     4981 aataccgtcc tctatttcat attgaggttc ccagtccaaa taatctaatg cccttgaatt
     5041 atccaagata ctgtgcctga tatctccttc tcttctttcc ttatagatcg gttctagctt
     5101 tttagccaat tcttcattga ttagcttgac caatttatta atagtggttt cagaacgggt
     5161 tgaaacattt aaagtttttt cacttgctgt atctaaagcc ttcaaattag cttctataat
     5221 atcatagaca tggataaaat cgcgggtctg ctttccatct ccatagataa taggtctttt
     5281 atcttctaac atcctatcga taaagatagc aattactccc ccttcaccgg cgggagactg
     5341 tcggggtcca taagcattcg aatatcttaa aaccgtataa tttaaatcgt aagcattatt
     5401 aaaatatttt aaataatgtt cgggtgtatg tttactgact ccataaggag acatcgcctt
     5461 cacgggatgt ttttcattga tcggtaagta atttggctct ccataaacgg ccgcggaaga
     5521 ggcataaact aatttttcaa tgtcatgttt tacacaggcc tgaaataggt tgatactgcc
     5581 ctggatattg acatgactgt catagatggg gtcatggact gacctcgtta catcgatctg
     5641 ggcccccaag tgaataactt ttctaattgc atagttatca aagatatcaa ataattttct
     5701 atcagttaaa tctatattat aaaattgggc tttctcattt acattttctt tgaagcctgt
     5761 cgaaagatta tcaacaatta ctacttcttc gcccctatca attagggcat ctaccatatt
     5821 tgaacctata aatccggctc ctcctgttac tagtatactc atcttttact tactcccccc
     5881 cattatttcg ttgcctattt ttttaaatac taaaactgtt cgtctttctt atccttaatt
     5941 ctttctatga ttaaaccaat tataaggatt ataagtccaa taatcaaacc aaatatacct
     6001 atcttaacta ataaactaag atcagtatca attaagaggt tcatgattaa atgataaatt
     6061 ccattaccca gtaaaaggac tgctgctaag attaagacaa ccaacccaat attttttata
     6121 ttcatctctt ttaatcctcc tctactttct ggataaagtt tataatataa tctacagtct
     6181 ggttccactg ctcttcttta ctaatttcac tttcgccatc cccttcctgg g
//
LOCUS       DATGCJ010000016         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_1866, whole genome shotgun sequence.
ACCESSION   DATGCJ010000016 DATGCJ010000000
VERSION     DATGCJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..894
                     /locus_tag="VKN64_00685"
     CDS             37..894
                     /locus_tag="VKN64_00685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HMA58774.1"
                     /translation="MKKEDKRSLIIYTVITFVFTWIFWITALYMGYKDIPFLRYLNWD
                     FKSSSQVTRHLIFRIGVYGPLVGSLIVTYLYSGGQGLRSFIKRIFKVKIKMKWYLYLF
                     LIPIIINFVVVLIGLIIGININSFFKSSIPINYILVFFIYEVLTSGLEEPGWRGFALE
                     KLQNSFTAEKTSWILGLIWAVWHYPYVISLYITSGFMPMVLSLAGFSMAIIGQTFIMI
                     WFYNNTNSIFVAILLHAWLNTSTTFVLGDITINNPIMGIIPALVTWAIVVVLLKVYGG
                     ETLTKESVK"
     gene            927..1847
                     /locus_tag="VKN64_00690"
     CDS             927..1847
                     /locus_tag="VKN64_00690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA58775.1"
                     /translation="MITSRLYKDSDYKKIMEFLSEMYQENKNQHCWLPARWEYAEHLV
                     NPLYVERGNKSWEPFIRIWEDGEKIVGISHPEDTYNAFLQIRPGYRDLEPEMIEWAEE
                     TIKKPISENKEKVVIWANETDDYRKEILNKRGYRKSEDCNYLNKQSLDKDFNSNLPAG
                     YKFRSMDEEISFVKRYNVINKAFHPENDFVKEVPESFVKMASAPMYRPYLDLIIEDQH
                     GNFASSCVIWFDEENKIGMFEPVGTHPDYTRKGLGRELLKEGLRRLKELGAETAYVES
                     YGDNRYAFYKSTGFKAYDKDYPWVKTFARE"
     gene            1872..2174
                     /locus_tag="VKN64_00695"
     CDS             1872..2174
                     /locus_tag="VKN64_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58776.1"
                     /translation="MSEYTYIQLLNKTLKYYFEEKYKEGYNFLNKHLDTVEGNQAQLY
                     NFLYTLACKSGQPEKAIELLKEAVYEKDYWYADQYLREDDDLDPIRECQLLPPNQK"
BASE COUNT          840 a          358 c          478 g          758 t
ORIGIN      
        1 aatattattg gataagggct gaagaaagga gtaaaaatga aaaaggaaga taaaaggtcc
       61 ttaataattt atactgtgat aacctttgtt tttacctgga ttttttggat tacggcctta
      121 tatatgggct ataaagatat ccccttttta aggtacctta attgggattt taaaagttcc
      181 agtcaggtta ccaggcatct tatctttagg ataggggtat acggcccatt agttggttcc
      241 ttaatagtaa cttaccttta ttcaggtggg caaggattga gaagtttcat aaaaaggata
      301 ttcaaagtta aaataaagat gaaatggtat ctttatttat ttttaattcc tatcataata
      361 aactttgtag tagtattaat aggtttaatt attggtatta atatcaattc tttttttaaa
      421 agcagtattc ctataaatta tatattagtg ttttttattt atgaagtatt aacaagtggt
      481 ttggaagaac cgggttggcg gggttttgcc ctggaaaaac ttcagaattc ttttacagcc
      541 gagaaaacca gctggatttt aggtttaatc tgggcggtct ggcattatcc ctatgtgatt
      601 tcattatata taacatctgg ttttatgccg atggtattgt cgttagcagg cttcagcatg
      661 gctattatcg ggcaaacctt tatcatgatc tggttttata acaatactaa cagtattttt
      721 gtggccattc tattacacgc ctggttaaat acttccacta cttttgtttt gggagatata
      781 actataaata accctattat gggtataata ccggccctgg taacctgggc aattgttgta
      841 gtccttctaa aagtttatgg aggagagacc ttaactaaag aaagtgtaaa ataatttttt
      901 taatgtaaag gactgaggtg aatatattga ttacaagtag attatataaa gattcagatt
      961 ataaaaaaat aatggaattt ttaagtgaaa tgtatcagga aaataagaat cagcactgct
     1021 ggttaccggc ccgctgggag tatgctgaac accttgttaa tcctctctat gtagaaaggg
     1081 gcaataaaag ttgggaaccc tttatcagga tctgggaaga tggtgagaaa atagttggta
     1141 tttctcaccc agaagatacc tataatgcct ttctccaaat aagacccggt tatagggatt
     1201 tagaaccgga aatgatcgaa tgggcagaag aaactataaa aaaaccaatc agtgagaata
     1261 aagaaaaagt tgtgatctgg gccaatgaaa ctgatgatta ccgtaaagag attttaaata
     1321 aaagaggcta cagaaaaagt gaagactgca attatttaaa taaacagtca ctagataaag
     1381 attttaattc aaacctaccg gccggttata aatttagatc gatggatgaa gaaataagtt
     1441 ttgtcaaaag atataatgta atcaataaag ccttccatcc tgaaaatgac tttgtcaagg
     1501 aagtaccgga gtccttcgtt aagatggcca gtgccccgat gtacaggcct taccttgatc
     1561 taataataga agatcaacat ggaaactttg cttcctcctg tgttatctgg tttgatgaag
     1621 agaataaaat aggtatgttc gaaccggtgg gaacccatcc tgactatacg agaaaagggt
     1681 taggtagaga attgctcaaa gaaggattaa gaagactaaa agaactgggt gctgaaacag
     1741 cctatgtaga atcgtatgga gataatagat atgcgtttta taaatccacc ggttttaaag
     1801 cttatgataa ggattacccc tgggttaaaa cttttgcaag agaataaaaa tataaaagag
     1861 gagataataa tatgtcagag tatacctata tacagctctt aaacaaaaca cttaagtatt
     1921 attttgagga aaaatataaa gagggttata attttttaaa taaacatttg gatacagttg
     1981 aaggtaatca ggcccaattg tataattttc tttataccct ggcctgtaaa tctggccagc
     2041 ctgagaaggc cattgaactt ctcaaggaag cggtttatga gaaagattat tggtatgcag
     2101 atcaatatct acgagaagat gatgacttag atcctatcag agaatgtcaa cttctcccac
     2161 ctaatcaaaa ataagattag gaaggagctt gttaaattaa aaaccgtact cagtacagtc
     2221 tttaacaaga tttacacctt ctctatctca catatgggtg attcaagaaa gagccagttg
     2281 aagggcagcg actaaaaacc ctatccctac tgtaaaaaca ataggggcta caggttaccc
     2341 tgtatgctat cattggatag ctcttttagc tatattaaca cttgcattat aatcagcatt
     2401 ttgtagatgt tgattatcac aaacaaattt attt
//
LOCUS       DATGCJ010000017        14031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_91892, whole genome shotgun sequence.
ACCESSION   DATGCJ010000017 DATGCJ010000000
VERSION     DATGCJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 14031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14031
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91892"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1341)
                     /locus_tag="VKN64_00700"
     CDS             complement(16..1341)
                     /locus_tag="VKN64_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HMA58777.1"
                     /translation="MKLELGNFHVEDIEFGEKTAFNDGLLTINKEEALEVVYKDEHIT
                     EADLKIVKPGDMVRLVPVKEAIEPRYREEGGPVFPGVTGDLMQAGDGRTLAMKDMSVL
                     VVGKHWGGFQDGLIDMGGEGAKYTYFSQLKNVVLVADTDEEFERHEQQKKNRALRWAG
                     MRLSEYLGETLKDQEPDEVESYSLDPITKRSKEVNDLPSVVIVMQPQSQMEEPGYNDL
                     NYGWDTNNMLPTFMHPNEVLDGAMISGSFMPCSSKWATYDFQNFPLIKRLYEEHGETI
                     NFLGVIMSNLNVAMDQKERSIQFVAQMAKSLGADAAVVTEEGYGNPDADYIGCLVALE
                     DAGVKTVGVSNEATGRDGGSQPLVSLDPKADALVSTGNVSELIKLPPMPEVIGELEAL
                     ARDGLSGGWEDDEVLGSSVKEDGSIIMENNSMFCGDQIAGWSPKTMKEF"
     gene            complement(1395..1862)
                     /gene="grdA"
                     /locus_tag="VKN64_00705"
     CDS             complement(1395..1862)
                     /gene="grdA"
                     /locus_tag="VKN64_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1737..1739),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HMA58778.1"
                     /translation="MLEGKKVAILGDRDGVPGPAIKECIESAGADVVFTTTECFVUTS
                     AGAMDLENQQRVKDLAEKHGKDNLVVILGGAEAEASGLACETVTNGDPTFAGPLAGVQ
                     LGLACYHILEPQIKDEVDEDVYDEQVSMMEMVLDVDDIVEEVSTYREKYSDFA"
     gene            complement(2074..3384)
                     /gene="grdB"
                     /locus_tag="VKN64_00710"
     CDS             complement(2074..3384)
                     /gene="grdB"
                     /locus_tag="VKN64_00710"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2335..2337),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HMA58779.1"
                     /translation="MGKYKIVHYLNQFFGQIGGEEKANIPPEVKEGPVGPGNAFNNAL
                     GEEAEIVKTIICGDSYFNENTEEVQNKVKEILQDVNPDLLIAGPAFNAGRYGVACGGV
                     AEVANDLDIPVVSGIYPENPGYDMYKQYAYFVETSDSAAGMRNALPDIVQLIKKYIEK
                     DGDLGLPKEEGYLPRGIRKNIFAEKQGSKRAVEMLIAKINGEEFETEYPMPDFDRVDP
                     VDPLEDVSNARIALVTSGGIVPKGNPDHIESSSASKYGKYSIEDIYEMTSESHETAHG
                     GYDPVYANEDPNCVLPVDVLKDMEEEGKIGKLHEYYYSTVGNGTSVANAKAYAQEIAQ
                     ELLEDGVQAVILTSTUGTCTRCGATMVKEIERAGIPVVHIATVIPISKTVGANRIIPA
                     VAIPHPLGDPNLAKAKQKAMRRIMVEKSINALETPVDSQTVFEG"
     gene            complement(3416..3886)
                     /gene="grdA"
                     /locus_tag="VKN64_00715"
     CDS             complement(3416..3886)
                     /gene="grdA"
                     /locus_tag="VKN64_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3761..3763),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HMA58780.1"
                     /translation="MLDGKKVAILGDRDGVPGPAIKECIETTGAEVVFTTTECFVUTS
                     AGAMDLENQARIKELTEKHGAANLVVILGGAEAEASGLACETVTNGDPTFAGPLAGVQ
                     LGLACYHILEPQIKDEVDEDVYDEQVSMMEMVLDVDEIIEETKKYREMYSEHAE"
     gene            complement(3913..5199)
                     /locus_tag="VKN64_00720"
     CDS             complement(3913..5199)
                     /locus_tag="VKN64_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HMA58781.1"
                     /translation="MKLELGKIQIEDISFGEKTEIKDSVLYINKEDLLENIDGEEDVK
                     SIEIDLVKPGDSVRIIPVKDVIEPRVKVEGPGGVFPGFIAGEEIVGTGRTIALKGCAV
                     VTTGNIVGFQEGIIDMTGPGADYTPFSQFNNIVINCEVKDDVQQHEHEELVRMIGLKA
                     ATYLAEAGKEVECDSVEEFEHKPFYEADNQYPDLPKVGYVYMLQSQGLLHDTYVYGVD
                     AKQILPTLISPTEIMDGAIISGNCVSACDKNATYIHQNNPVIKDLYERHGKDFNFMGV
                     IITNENVTLADKERSSNYTAKLAEQMGLDAAIVSEEGFGNPDADLIMNCTKLEEKGIK
                     TVLITDEYAGRDGSSQSLADADPRADAVITAGNANEVITLPPLEKTIGYQKTANVIAG
                     GFDGNIEDDGSITVEIQAITGSTNELGLNKLTARGY"
     gene            complement(5215..5541)
                     /locus_tag="VKN64_00725"
     CDS             complement(5215..5541)
                     /locus_tag="VKN64_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA58782.1"
                     /translation="MLELNKDNFESEVLDSEGVVLVDYWSDNCDLCLDLMPDVEELAE
                     KYGDKIKFAKLNIKGNRRLAIGQKVLGLPSLVFYEDGEKKEHLSGDELEPEDIEEVLK
                     KYYEQA"
     gene            complement(5577..5963)
                     /locus_tag="VKN64_00730"
     CDS             complement(5577..5963)
                     /locus_tag="VKN64_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HMA58783.1"
                     /translation="MSKYILITNNPKVKNKYEEKNIKISFMDNIESIFKEVRDYIHQG
                     YILISHPLAGSVKPFQNPYRSIILRGNKGLDFDSLKTYENSYEKYQQFKEKKKSKSEL
                     PADILDDYQVIDLSLIESAIQSIKLD"
     regulatory      complement(6012..6106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(6297..6557)
                     /locus_tag="VKN64_00735"
     CDS             complement(6297..6557)
                     /locus_tag="VKN64_00735"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HMA58784.1"
                     /translation="MEYYLITFASTHHYMRAEKLLDDMDYEFELLNIPPEISADCGLG
                     LKFEAVVDYDQVHTIIKRDGIQIEGLYHIEKKDNEKNIEKIR"
     gene            complement(6568..7608)
                     /gene="selD"
                     /locus_tag="VKN64_00740"
     CDS             complement(6568..7608)
                     /gene="selD"
                     /locus_tag="VKN64_00740"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7561..7563),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HMA58785.1"
                     /translation="MTDKVRLTQYSKTSGUAAKLGPRALSQVLRQVSFENTDQNLMVG
                     IETGDDATVYKIDEERALIQSVDFFTPVVDDPYLYGQIAASNAISDIYAMGGKPFLAM
                     NIVGFPECLSQDLLVKILQGGADIVKKAGASMAGGHTVKDDEPKYGLSVTGFVHPDNL
                     LTNSNAQVGDKLILTKPLGTGVIISAIKGGLEEGGLDNEAVKSMVELNDKPVEVLKEY
                     KVNACTDITGFGLMGHLTEMVKGSNVQIEIETEEVPIFKGSIEYAEMGLLPEGAYSNK
                     ENYEEMVIKKYNINESIEDLLYDPQTSGGLLLAVPEESASDLLIELYGLGVDKAAVIG
                     EVKEGNKGIVLR"
     gene            complement(7643..8215)
                     /gene="yedF"
                     /locus_tag="VKN64_00745"
     CDS             complement(7643..8215)
                     /gene="yedF"
                     /locus_tag="VKN64_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HMA58786.1"
                     /translation="MEKIDARGLSCPKPVVKTKKALEDFEEVEVLVDNEVAAQNVKKL
                     GIKMGGNVTMVKEKEDLYKLVITDFEKTNQQSRKGEKDKVIFIKSELLGEGDEELGEL
                     LIRGFINTLLDIEPIPAKIIFMNSGVKLACHNQEIINSLKKLENKGVNVLACGTCLDF
                     YGLKEELEVGSISNMYEIVDSLNKGEVLEI"
     gene            complement(8376..9416)
                     /locus_tag="VKN64_00750"
     CDS             complement(8376..9416)
                     /locus_tag="VKN64_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMA58787.1"
                     /translation="MESSLNGYLKLSITLIISILLVSVITTNALAIGLELDDRGDKVR
                     EVQTLLKKVGYDISTDGIFGHNTKEIVKDFQLNNNLKVDGIVGDNTYSKLKKMAEDIK
                     YVVKKGDTLYGIAKRFDTTVETIRASNNLRGTMIHPGDTLYIPKTGRGGGDENRIYAN
                     IIHEVQPGDALLSIAKKYGVDVQTIKQANNLNSNMIRAGENLVIPFEERVKGGKFTLG
                     KGAFIWPLQGRISSYFGWRNDPVTGGSEFHKGLDIAVPLGTQIRAAASGEVVKSGWMS
                     GFGKTIVIDHGEGVETIYAHNSQNHVRVGEQVNTGQIIGRAGSTGKSTGSHLHFGVMV
                     DDKAVNPTKYLP"
     gene            complement(9508..9993)
                     /gene="trmL"
                     /locus_tag="VKN64_00755"
     CDS             complement(9508..9993)
                     /gene="trmL"
                     /locus_tag="VKN64_00755"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HMA58788.1"
                     /translation="MNIVLVEPEIPQNTGNIARTCAVTDCSLHLVRPLGFSTDDKYLK
                     RAGLDYWHMLDIHYYEDFETFLEEKKDCRMFFASTKSPIRYDQADYMQGDYLVFGKET
                     AGLPESFLKKELENTVRIPMVEGARSLNLSNSVSIILYEALRQNNFPNFKEKAKFSKY
                     Y"
     gene            complement(10008..10766)
                     /locus_tag="VKN64_00760"
     CDS             complement(10008..10766)
                     /locus_tag="VKN64_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA58789.1"
                     /translation="MEITFLGTGTSHGVPIVGCNCKTCTSNDPKNNRFRSSIYIKNSD
                     KNILIDTTPDLRLQLLNNDITDIDIVLFTHAHADHIMGFDDLRAINRLNKADIPCYGN
                     KETLDALKRKFAYIFKDHHCKFAVPSVKLIEIEGVLEFNNLEITPLPVKHNNDTVLGY
                     KVNNMAYITDCSCIPEESFNLIEDVDLLILDALRYKSHPKHFNIEEAVEVIKDLGVKR
                     AYLTHISHEIEHNEVNDKLPEGIQLAYDGLKLKV"
     gene            complement(10942..11526)
                     /locus_tag="VKN64_00765"
     CDS             complement(10942..11526)
                     /locus_tag="VKN64_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HMA58790.1"
                     /translation="MDVKKITNIGVLIGLSLILMLTIRFPLFLPFLIYEPGDIPILII
                     AFLYGPMPALGATFVLSVLMAALTGLGGPFGAFMHFLATGVLVGAAGYVYKKYHTKRG
                     ALLGLAAGSVAMTGVMAVANVLLNPIFYGIPYESTINMLLPGIIPFNLTKAIINSLLT
                     VLVYKKIADFLRSKGLLTSLECEESKDYVCVDEK"
     regulatory      complement(11615..11728)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11783..13954)
                     /locus_tag="VKN64_00770"
     CDS             complement(11783..13954)
                     /locus_tag="VKN64_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HMA58791.1"
                     /translation="MKKFLTVVIVLIICFFIGFSIPYIYNNYGPLLQKYVNGNGTTKI
                     ENGSSQKPIESDQPEEEPLKKEVDYDKLLAEAEDQFYEGNYDQSIDTYIKLYKEGYDK
                     LSVIKNIVSISDYSDNRSDQIDSILAATKQENNDSLDFNYYYGLYLYQKGKIEEAKDI
                     FNHLIVLLDEKESSLSNRKISLMYYYLGNIYNDQNEQEAALDFYQAGIEKNKEMVLNY
                     LAAADIYNNQDNYETAIEMYQAALSQDHSLSSLYYELALLFEKKGELLEAYHYWDRCV
                     NSGIETAKARERIKDIRKKYPEHFDKRDEAQPVTDIDWFAIEEIETNQEYEEIRIGLQ
                     EDIDRIRFQSKNDFTISQANNEIFRGESNTQYQINYNNHTFYILKDNKILERVTTNRK
                     LKIHSNDQNNLFALYDIKFAQNYFWGGTEDRQYRGDIYLNPVSNQYVDLLNHVDLSSY
                     LLSVVPSEMPASWPLEALKAQSVVARSYIIRNINRHQDEGYDLCASVHCIVYSGAKNE
                     HQSTTEAIIATRNEIITHNGNVIDAVFSSNSGGYTEASENVWINSLDYLKAVNTAKNN
                     SYNFPLAPYQVENWFVEKPASYSNNKYTTQSSYRWVKRIDLARLKERHGLEAIKRVYI
                     TDRTTTGTVKEIKIEGVNKTVTKNNSAIRRALTGLKSNKFIVKNIFEQGQLKSIIIFG
                     AGWGHAVGMDQTAAAGMAVEGKGYKEIIDYFYPDTKITKLE"
BASE COUNT         4122 a         2983 c         2036 g         4890 t
ORIGIN      
        1 attcacctcc cggagttaaa attctttcat tgttttgggg gaccagccag caatttggtc
       61 tccacagaac attgaattat tttccataat tattgagcca tcttctttta cagatgagcc
      121 tagtacttca tcatcttccc atccaccgga taaaccgtct ctggctaatg cttctaattc
      181 gccgataact tccggcatcg gtggaagttt aattagttcg gagacattac ctgttgatac
      241 taaagcatca gctttagggt ctaatgaaac gagaggctgt gaaccgccgt cacgaccggt
      301 agcttcatta cttactccaa cagtcttaac accagcatcc tctaatgcaa ctaaacaacc
      361 aatatagtca gcatcaggat taccgtatcc ttcttcagtt actactgcag catctgctcc
      421 gagagatttg gccatctggg caacaaattg aatagatctc tctttttggt ccatagctac
      481 atttaggttt gacattatta cacctaaaaa gttaatagtt tcaccatgtt cttcatataa
      541 tcttttgatc aaaggaaagt tctgaaagtc ataagtagcc cactttgaag aacagggcat
      601 gaaactacca gaaatcatag caccatctaa tacttcatta ggatgcatga aggtcggtag
      661 catattattt gtgtcccaac catagtttag atcattgtat ccaggttctt ccatctgaga
      721 ttgaggctgc attactataa caacacttgg tagatcatta acttctttag atctctttgt
      781 aattggatct aaggaataag attcaacttc atcaggctct tgatccttta aagtctctcc
      841 aaggtattca gataagcgca taccagccca tctcaaggca cgatttttct tttgctgttc
      901 gtgtctttcg aattcttcat cagtatcagc aactaaaact acattcttta attgagagaa
      961 gtatgtatat ttagcacctt ctcctcccat atcaattaag ccgtcctgga atcctcccca
     1021 atgtttaccg acgacaagta cactcatatc tttcatcgca agtgtcctac cgtcaccggc
     1081 ctgcattaaa tcaccagtta caccgggaaa taccggtccc ccctcttcac gataacgggg
     1141 ttcaattgct tctttaaccg gaaccaggcg gaccatatca ccaggcttta ctattttcaa
     1201 gtcggcttca gtgatatgtt catctttata aacaacttct aaagcttctt ctttattaat
     1261 agtaagaagg ccgtcattaa aagctgtttt ttcgccaaac tcaatatctt ctacatgaaa
     1321 attaccgagt tctagtttca ccttagtcca cctcttttct taaagtttaa tttgttattt
     1381 tttaacagta ggaattaagc aaagtcagaa tacttctcgc ggtaagtaga tacttcttct
     1441 acgatatcat ctacatctaa aaccatttcc atcattgaga cctgttcgtc ataaacatct
     1501 tcatcaacct catctttaat ttgaggttct agaatgtggt agcacgcgag ccccaactgg
     1561 actcctgcca atggacctgc aaaggtagga tctccgttag ttactgtttc acaggctaag
     1621 ccagaagctt ctgcttccgc tccacctaaa ataactacta agttatcctt tccatgtttc
     1681 tcagctaaat ctttcacacg ctgttgattc tcgaggtcca tggcgcctgc gcttgttcag
     1741 acgaaacatt ctgtagtagt aaatactaca tctgcaccag ctgattctat acattcttta
     1801 atagctgggc caggtacgcc atctctgtca cctaaaatgg caactttttt accttcaagc
     1861 atcttattga cacctccata atttttatag tctttttcaa ttttcacaaa attataataa
     1921 cataatcatg attatatatc aaccatgatt gtgaaaaaat ttatttattt tatgattttt
     1981 ttcactttgt tttgatatat tttcttaact attaaaaaga ggctgcagcg taaactacag
     2041 ccccttaaat aatatctttt ctaatttttt ttcttatccc tcaaacacag tttgtgaatc
     2101 aactggagtt tctaaagcat ttattgattt ttctaccatt attcttcgca tcgctttttg
     2161 tttagctttt gccagattgg gatctcccaa gggatgtgga attgctacag caggtattat
     2221 tcgatttgca ccaactgttt ttgaaattgg tatcacagta gcaatatgaa caactggaat
     2281 tccggctctc tcaatctctt tcaccattgt tgcaccgcaa cgtgtacagg tacctcaggt
     2341 agaggtgagt ataacagcct gaactccatc ctccaataac tcttgagcta tctcctgtgc
     2401 atatgctttt gcatttgcaa cagaagtacc attacctact gttgaataat agtattcatg
     2461 taatttgcct attttacctt cctcttccat atcttttagt acatcaactg gtaaaacaca
     2521 gttcggatct tcattagcat aaactgggtc atagccgcca tgagctgttt catgggactc
     2581 tgaagtcatt tcatagatat cttctatact gtatttacca tatttagaag cactggatga
     2641 ttcaatatga tcaggattac ctttaggtac aataccgccc gatgtaacta aggcaattct
     2701 agcattagaa acatcttcga gtggatcaac aggatctaca cgatcaaaat ccggcattgg
     2761 atattctgtt tcaaattctt caccatttat tttggctatt aacatttcta cagctctctt
     2821 ggaaccctgt ttttcagcaa agatattttt tctaattccg cggggaagat aaccttcctc
     2881 ttttggtaaa ccaagatcac catctttttc aatatatttt ttaataagtt gaacaatatc
     2941 tggtagagca tttctcatgc ctgctgcgga atctgaagtt tccacaaagt aagcatactg
     3001 cttatacata tcataacccg ggttttcggg ataaattccg gaaacaaccg gaatatctag
     3061 atcatttgct acttctgcca ccccaccaca agctactcca taacggcccg cattaaaagc
     3121 aggacctgca attaataaat cggggttgac atcctgtaaa atctctttta ctttattttg
     3181 cacttcttcg gtattttcat tgaaatagct atctccacaa attatggttt ttactatttc
     3241 agcctcttct cctaaagcat tgttaaaagc attaccgggt ccaaccggtc cttcttttac
     3301 ttcaggagga atattcgcct tttcttctcc cccaatttgt ccaaaaaatt ggttgagata
     3361 atgtacaatt ttatattttc ccatttaaca tacgccccct ttcaactggg aacaattatt
     3421 cagcatgttc agaatacatt tccctatatt ttttagtttc ttcaataatt tcatcaacat
     3481 ctaagaccat ttccatcatt gatacctgtt cgtcataaac atcttcatca acctcatctt
     3541 taatttgagg ttctagaata tggtagcacg cgagccccaa ctggactcct gccaatggac
     3601 ctgcaaaggt tggatctcca tttgtaactg tttcacacgc gagaccagaa gcttctgctt
     3661 ctgctccacc taagataaca accaggttgg ctgcaccgtg tttttcagtt aactctttaa
     3721 tcctagcttg attttccaga tccatggcgc ctgcgcttgt tcagacgaaa cattcagtgg
     3781 ttgtaaagac aacctcggca ccggttgttt cgatacattc ttttatagct ggcccgggaa
     3841 ctccgtctct atcgcccagg atagcaactt ttttaccatc taacatttaa cttgcacctc
     3901 ctaaatattt ttttaataac ctcttgctgt taatttattc aatcctaatt catttgttga
     3961 accagttata gcctgaattt caacagtaat agaaccatca tcttcaatat ttccatcaaa
     4021 gccaccggca attacattag ctgttttctg atagccaatg gttttttcca gcggtggtaa
     4081 ggtaataact tcattagcat taccggctgt tataactgca tcagctctcg gatcggcatc
     4141 tgctagagac tgtgaacttc cgtctctgcc ggcatattca tcagtaatta gaactgtttt
     4201 gatgcctttt tcttctaact tggtacagtt cataattagg tctgcatcgg gattaccgaa
     4261 gccttcttct gaaactatag ctgcatcaag gcccatttgt tctgccaatt tagcagtata
     4321 gttagaagat ctttctttat cagccagggt aacgttctca tttgtaatta ttactcccat
     4381 aaagttaaag tctttcccat gtctttcata taggtcttta attacagggt tattttgatg
     4441 gatatatgtt gcatttttgt cacaggctga tacacagtta ccacttatga tagctccgtc
     4501 cattatttcg gtaggagaaa ttaatgttgg tagtatctgc ttggcatcta caccatatac
     4561 ataggtatca tgtagtaagc cctgactctg cagcatataa acataaccca ctttaggtaa
     4621 atcaggatac tgattatcag cttcatagaa tggtttgtgt tcaaactctt ccacagagtc
     4681 acattctact tctttacctg cttcagccaa ataagtggct gcttttaaac caatcattct
     4741 gacaagttct tcatgttcgt gctgctgtac atcatctttt acttcacagt taattacaat
     4801 attattaaac tgtgaaaaag gagtatagtc agcacccgga ccggtcatat caatgatccc
     4861 ttcctggaaa cctacaatat taccggtagt aactactgca catcctttta atgcaattgt
     4921 gcggccggtt ccaacaattt cttcccctgc gataaaaccg gggaatactc caccggggcc
     4981 ttctaccttt acccgcggtt caataacatc tttaaccgga ataatacgaa cactatcacc
     5041 tggtttaact aaatcaatct cgatagactt aacatcttct tctccatcga tattctccag
     5101 taaatcctct ttattgatat ataaaacact atctttgatc tctgttttct caccaaaact
     5161 gatatcttca atttgaattt ttcctaattc taacttcaca tcttcacccc tttcttaagc
     5221 ctgctcataa tattttttaa gtacctcttc tatatcttca ggctctaatt catctccact
     5281 gaggtgttct tttttctccc catcttcata gaaaactaga gaaggtaaac ctaacacctt
     5341 ttgtcctatc gctaaacgac gatttccttt aatatttaac tttgcaaatt taattttatc
     5401 tccatacttt tcagccagtt cttctacatc tggcataagg tctaaacaaa gatcacagtt
     5461 atcgctccaa taatctacta atacaacacc ctctgagtct aaaacttcgg actcaaagtt
     5521 atctttattt aactctaaca tctcttatca cctcccctta taagattaat ataatatcag
     5581 tctaatttaa tagactgtat agcactttca atcaatgata aatcaattac ctgataatca
     5641 tctaaaatat cagcgggtag ttcagatttg gacttctttt tttctttaaa ctgctggtat
     5701 ttctcatatg agttttcata agtttttaac gaatcaaagt ctaaaccctt atttcccctt
     5761 aagataatag accgataggg attttggaaa ggcttcacac tacctgctaa tggatgtgag
     5821 attaaaatat atccctgatg aatgtaatca cggacttcct tgaaaatact ttcaatatta
     5881 tccataaaac ttatttttat gtttttttct tcatatttat tttttacttt tggattattt
     5941 gttatcaata tatatttgct catgatagct cctttttaaa atcaaaaaaa gacagaatag
     6001 taatgataga ccattactac tctgtcttta tacctgagag attacctaat cttataacta
     6061 gattgctcct tcggtgttta ataataaact ttccagagtt ctgtcagaag acggtccttt
     6121 tacctgaaag tttcattccc ctacatatgg catcagggaa tttacccctt cggtggttat
     6181 aaaaacgctc tcccatcttc ttcaaccatc tttatttgat tataaaatat ttttttcaca
     6241 aagtctgatg ttattatata agataggttg aggcttgtca acaaaattga gcaattttat
     6301 cttatttttt ctatattttt ttcattatcc ttcttttcaa tatggtaaag accttcaatc
     6361 tgaataccgt ctcttttaat aatcgtatgt acttgatcat aatcaactac agcctcaaac
     6421 ttcaaaccca gaccacagtc agcactaatt tcaggcggga tatttaaaag ttcaaattcg
     6481 taatccatat cgtcgagcag tttttctgct ctcatataat gatgagttga tgcaaatgta
     6541 atcagatagt attccaatat tatcacctta ccttaatact attcctttat taccttcttt
     6601 gacctcgccg atcacagctg ctttatctac tcccagtcca tataattcga ttaatagatc
     6661 agaagctgat tcttctggta cagccaataa aagaccgccg gaagtctggg gatcatataa
     6721 gaggtcttca atactttcat tgatattata tttttttatt accatctctt cataattttc
     6781 tttatttgag taggcgcctt cgggcagtaa ccccatctcg gcatattcaa tggagccctt
     6841 aaagatcggc acctcttcag tttctatttc gatttggaca ttgctgcctt tgaccatctc
     6901 ggtcagatgc cccataagtc caaaacccgt aatatctgta caggcattta ccttatattc
     6961 ctttaaaact tcaaccggct tatcatttag ttcgaccatc gatttaactg cttcattatc
     7021 taggccgcct tcttctaagc cgccttttat ggcagaaatt attactcctg tccccagggg
     7081 tttggttaag attaatttat ctcccacctg tgcattggag tttgttagta aattatcggg
     7141 atggacaaaa cctgtcactg ataaaccgta cttcggttca tcatctttaa cggtatggcc
     7201 gcccgccatt gaggcaccgg cctttttgac tatgtctgca ccgccctgta atattttaac
     7261 taatagatcc tggctcaaac attctgggaa accgacaatg ttcatggcca gaaaaggttt
     7321 acctcccatc gcatagatat cacttatggc attcgaagca gcaatctgtc catatagata
     7381 gggatcatca accacgggag taaaaaaatc taccgattga atcaaagccc tctcttcatc
     7441 tattttatag acagttgcat catcacctgt ttctatacct accatcagat tctggtctgt
     7501 attttcaaag ctaacttgtc gcaaaacttg cgataaggcc ctagggccta atttagctgc
     7561 tcagccggaa gttttactgt actgtgttaa tcttacttta tcagtcataa gttcaccacc
     7621 ttaatttata tttaatcact tattatattt ctaatacttc tcctttattt aaactgtcaa
     7681 ctatctcata catattactt atactgccta cttctaattc ctcctttaga ccatagaaat
     7741 caagacaggt accacaagcc agtacattta ctcccttgtt ttctaatttc ttgagggaat
     7801 taattatttc ttgattatga caggcaagtt ttacaccgga gttcataaaa ataatcttgg
     7861 ccgggatagg ttcaatatca agaagggtat taataaatcc cctaattaat agttcaccta
     7921 attcctcgtc accttcccct aataattcag atttaataaa gatcacttta tctttttctc
     7981 ctttgcggct ctgttggttt gttttctcaa aatcagttat taccaattta tatagatctt
     8041 ctttttcttt aaccattgtt acattcccgc ccattttaat acctagcttc tttacatttt
     8101 gggcagcaac ttcattatca actaatactt ctacttcctc aaaatcttct aaagcctttt
     8161 tagttttaac aaccggttta ggacaggata aacctcttgc atctattttt tccatcttgt
     8221 gacctcctta acaaatttta tttaaaatta tattatcatt tattatgtat tatatcaatt
     8281 ttttttatag aatatgtaaa tttttttata aaaaacagta aaaaaacctc ataccaaact
     8341 gatatgaggt gatttacaat ttatatctat tttcattatg gtaaatactt tgttggatta
     8401 acagctttat catcgaccat tacaccaaag tgtaaatgag atccggtaga tttaccggta
     8461 ctaccggctc taccaataat ttgacctgta ttaacctgct ctcctactct aacatggttc
     8521 tgagaattgt gggcataaat tgtttcaaca ccttcaccgt ggtcgataac gatagtcttt
     8581 ccaaaaccac tcatccagcc gctttttaca acttcgccac tagctgcagc tctaatttgc
     8641 gttcccaggg gaactgcaat atctaaacct ttatgaaatt cactaccacc agtaactgga
     8701 tcattcctcc agccgaaata ggaagatatt cttccctgga gaggccagat aaaagctcct
     8761 ttacccagtg tgaatttacc tcctttgact ctttcttcaa agggtataac taaattttca
     8821 ccggctctta tcatattaga atttaaatta ttagcctgtt tgatagtctg tacatcaaca
     8881 ccatactttt tagctatcga aagtagggca tctccgggtt gaacctcgtg gataatgtta
     8941 gcatatatgc ggttttcatc tccacctcca cgcccggttt taggtatata aagagtatct
     9001 ccggggtgaa tcatggtacc tcttaaatta ttgctggctc taatagtctc taccgttgta
     9061 tcaaatcttt ttgcaatacc ataaagtgta tcgccttttt taactacata tttaatatct
     9121 tcagccatct tttttaactt actatatgta ttatctccaa ctatcccatc aacttttaaa
     9181 ttattattca gttggaaatc cttaactatt tcttttgtat tatgaccgaa gataccatcg
     9241 gtactgatat cataaccgac cttttttaag agagtttgaa cttctcttac tttatctcct
     9301 ctatcatcta attctaaacc aattgctaaa gcattagttg taattactga aactaatagt
     9361 atagaaatta ttagtgtgat acttaatttc aaatatccgt ttaagcttga ctccatatcc
     9421 tcaatcctcc tagtttagaa tctttttacc cctagtatat ttaattctag ctatttttaa
     9481 atattccttt aagaaaactg taaaaattta atagtatttt gaaaactttg ccttctcttt
     9541 aaaattaggg aaattattct gtcttaatgc ttcataaagt ataatcgaaa ccgaatttga
     9601 taaattaagt gaacgtgccc cttcaaccat gggaatcctt accgtatttt ccaattcctt
     9661 ttttaaaaac gattcgggta aacctgccgt ctctttacca aagaccagat aatctccctg
     9721 catataatct gcttgatcat acctaatagg agatttagtt gaagcaaaaa acatccgaca
     9781 gtcctttttt tcttccaaaa aggtctcaaa atcttcataa taatgtatgt ccaacatgtg
     9841 ccagtaatcc aaaccagccc tttttaagta cttatcatca gtagaaaaac ctaagggccg
     9901 aacaaggtgt aaactacaat ctgttaccgc acaggttctt gcaatattac ctgtattttg
     9961 gggaatttct ggttccacta atactatatt catcaattta tcatccttta aactttaagc
    10021 tttaaaccat cataggcgag ctgaatccct tcaggtagtt tgtcatttac ttcattgtgt
    10081 tcaatttcat gtgaaatatg ggttaaataa gcccttttta ctcccagatc tttgattact
    10141 tctactgctt cctcaatatt aaaatgcttg gggtgactct tataacgaag tgcatctaaa
    10201 attagtaaat cgacatcttc aattaaatta aaactctctt ccggaataca gctgcaatct
    10261 gttatataag ccatgttatt taccttataa ccgagaacag tatcattgtt atgctttacc
    10321 gggaggggtg taatctctaa gttattgaac tcaagtactc cttcaatttc aattaacttg
    10381 acactgggaa ctgcaaactt acagtgatgg tctttaaaaa tataagcaaa ctttcttttt
    10441 agtgcatcta gggtctcttt attgccataa caggggatat cagctttatt taaacgattg
    10501 attgccctta aatcatcaaa acccattatg tgatctgcat gggcatgggt aaataaaact
    10561 atatcaatat ctgtaatatc attattaagt aactgtaatc tcaaatccgg tgtagtatct
    10621 attaatatat tcttatctga atttttaata tatattgaag atctaaaccg attgttttta
    10681 ggatcattag aagtacaggt tttacaatta caaccaacaa tgggaactcc gtgtgaagtc
    10741 cctgtgccta agaatgttat ttccaataaa attacctcca atacacttat ataaaattgt
    10801 atattagtca agctaaattg tcaaataatt aacccaacaa atgctattat cacaagcttt
    10861 ttacaatctt aactaaaaga aaaatccctt cacagaaaaa aatctataaa gggatgaaaa
    10921 attatattta ttttttaata attatttctc atcaacacaa acataatctt tactttcttc
    10981 acactctaaa gaggtcagta atcctttact tcttaagaag tcagctatct tcttatagac
    11041 aagtaccgtt aatagagagt taattatcgc ctttgttaag ttaaagggaa taatccccgg
    11101 taataacata ttaatagtac tttcataagg aataccataa aagatggggt tcaataatac
    11161 attggcaaca gccattacac ccgtcattgc aactgatccg gcagctaaac ccagtaaagc
    11221 acctctttta gtatggtact ttttatagac atagccagct gctcctacca aaacaccggt
    11281 ggctaaaaag tgcataaagg ctccaaaagg accacccaaa ccggttaagg ccgccattaa
    11341 aacggaaagt acgaaagttg cccctaatgc aggcatcgga ccatataaaa atgcaataat
    11401 taagatcgga atatcacctg gttcataaat taaaaaaggt aggaaaagag gaaatctaat
    11461 ggtaagcatt aaaattaatg ataacccgat taaaactcct atgttagtaa ttttcttgac
    11521 atccatatat tttttattcc tcctaaaata atatttagaa caaataaaaa attgccctgc
    11581 agaagacagg gcattagttt ctttataatt aataacgcgc cttctctcat ccggactgtt
    11641 taccgtcggt actggaaatt aaccagttca gcgttagctc gtggacttta ccaccggtct
    11701 ggaattacac catgccctga agacaatttt ttattcaatt cttgaaataa ttataatata
    11761 aaatgttaat catttcaaga aattattcca actttgtgat ttttgtatca ggataaaaat
    11821 aatcaattat ctctttataa cctttaccct ctacggccat gccagccgca gctgtttggt
    11881 ccatacctac tgcatgaccc cagccggctc caaagatgat aatacttttt aactgacctt
    11941 gttcaaatat atttttaact ataaatttat tgctttttaa accggtcaaa gcccttcgga
    12001 tggctgaatt gtttttggta accgttttat ttacaccttc aatttttatc tcttttacag
    12061 taccggtagt tgttctatct gtaatataga ctcttttaat ggcttctaaa ccatgcctct
    12121 cttttaatcg ggccaggtct atccttttaa cccagcgata gctgctctgg gtcgtatatt
    12181 tattattgga ataggaagca ggcttttcaa caaaccagtt ttcaacctga tatggtgcaa
    12241 gcgggaaatt ataactatta tttttagccg tattgactgc tttaagatag tcaagactat
    12301 ttatccagac attttcactt gcttctgtat accccccact attactactg aacacagcat
    12361 ctattacatt gccattgtgg gtaattatct catttctggt ggcaatgata gcctcagtag
    12421 tactttggtg ttcatttttg gccccggaat agactataca gtgaacactt gcacaaagat
    12481 cataaccttc atcttgatgt ctgttaatat ttctaattat ataggacctg gctaccacac
    12541 tttgggcttt cagggcttcc aagggccaac tggccggcat ttcagatggt acaacagaaa
    12601 gcaggtagga agaaaggtcc acatggttta ataggtccac atattgattt gaaacagggt
    12661 ttaagtaaat atcaccccta tactgtctat cttctgtacc tccccaaaaa taattttgcg
    12721 caaacttaat atcataaaga gcaaacaggt tattttgatc atttgaatgt atttttaact
    12781 tacgattggt ggttaccctt tctaatattt tattatcttt taaaatatag aaagtatggt
    12841 tattgtagtt aatctgatac tgggtattgc tttctccccg gaaaatttcg ttatttgcct
    12901 ggctaatagt gaagtcgttt tttgattgaa accttatccg gtctatatct tcctgtaagc
    12961 ctatccgtat ttcctcatac tcctggttag tttcaatttc ttctatcgca aaccagtcaa
    13021 tatcagttac aggttgagct tcatctcgtt tatcaaaatg ttccggatat ttttttctaa
    13081 tgtcttttat tctttcccgc gccttagcgg tctcaatccc actgttcaca caacggtccc
    13141 aataatgata agcctctaat aattcccctt ttttttcaaa taaaagggca agttcgtaat
    13201 ataggctgct taaactgtgg tcctggctta aagcggcctg atacatctca atagctgttt
    13261 cgtaattatc ctggttatta tatatatcag ctgctgccag ataatttaag accatctctt
    13321 tatttttttc aataccggcc tgataaaaat ctaaagcagc ctcttgctca ttttggtcat
    13381 tatatatatt acctaagtaa taatacatta aagaaatttt tcggttagat aaactacttt
    13441 ctttttcatc taacaagacg attaaatgat tgaaaatgtc tttagcttct tctattttac
    13501 ctttttgata aagataaaga ccataatagt aattaaaatc taaagaatca ttattttcct
    13561 gttttgtggc tgctaaaata ctatcaatct gatcagaccg attatcacta taatcactta
    13621 tggatacaat atttttgatt actgagagtt tgtcataacc ttccttataa agttttatat
    13681 aagtatcaat gctttgatca taatttccct cataaaattg gtcttccgcc tcagctaaca
    13741 atttatcata atcaacttct tttttgaggg gttcctcttc tggttgatca ctttctattg
    13801 gtttctgtga actaccattt tctattttag tagtcccatt accgttaaca tacttttgaa
    13861 gtaatggacc ataattgttg tatatatatg gaatagaaaa tcctataaaa aaacatatta
    13921 ttaatacaat tactactgtt aaaaattttt tcattattaa accaccttat ttaactgcta
    13981 tataataatt tcgataagaa agcttctatc cctgctaaaa aaagaaaaag t
//
LOCUS       DATGCJ010000018        10148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_2422, whole genome shotgun sequence.
ACCESSION   DATGCJ010000018 DATGCJ010000000
VERSION     DATGCJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VKN64_00775"
     CDS             <1..897
                     /locus_tag="VKN64_00775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMA58792.1"
                     /translation="FIEFFTIYDLRKEYNLSAQSSKEWLNDRVKSFLLTLVLTHLFSL
                     LFLFLTNNYPQAWWLIMSIILSVFVVLLNYLLPVLILPLFYTLKSYPEGELKDRLMST
                     FDKLDIEIEDIYEINLSSKMTSANAAVIGMGKTRKILLSDNLADRFSAEEIEAVLSHE
                     IGHHVNNDIYKNLLLLPFIIVITAFIVHLSWPTLLSWFGYVNQNLIISLPLLLLYWGI
                     LYSIFTPLQLYLSRKYERRADKFAFEVIDKPMNLGKAFAKLADDSLSRLDYRWWEKLY
                     KASHPSIKSRIYEAKEHKDNND"
     gene            970..2013
                     /gene="mtnA"
                     /locus_tag="VKN64_00780"
     CDS             970..2013
                     /gene="mtnA"
                     /locus_tag="VKN64_00780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMA58793.1"
                     /translation="MEHKTLYFEDGVLKLIDQRILPLETQYYEASDYREVCEAIKKMV
                     VRGAPAIGATGAYGYYLAVREHISADNVIFLDEIEKAKEELLATRPTAVNLSWGIRRM
                     EKVLKECLAKDYNEDEIEESLLQEANKIAQEDIKTNKRLGRFGNQLIEKGDNILTHCN
                     AGALATVGYGTALGVIRAAHAEGKNIKVYMDETRPRLQGASLTAFEMLEEDIPATLIV
                     DSTAATLMKKGVIDKVVVGADRIAKNGDTANKIGTFMLSVLAKEYDVPFYIAAPLSTV
                     DFEIDSGDDIEIEIRADKEVTDIRSVRIAPVGVDVYNPAFDVTPAENITAIITEKGVI
                     EPPFTENLNKARK"
     gene            2063..2821
                     /locus_tag="VKN64_00785"
     CDS             2063..2821
                     /locus_tag="VKN64_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMA58794.1"
                     /translation="MEDLNSIAIISDIHGNYTALENVLKKIEMYDEQINDIMCLGDLV
                     GYNPEPEKCVNKIREVCSKVIAGNHDKAMVGELNIQWFRQTVKKSITWTRDHLSEESK
                     SYLKSLNTREDLTINGRKIILAHGSPDQNSPFSYIFNRYDIKAIEPLIMDTNILFIGH
                     THIPFCFCNKGDGWQQIEKDEIEINPEYNYIVNVGSVGQPRDRDPRLSFVIFNPNNNL
                     LTFHRYEYNIKEVQDKFAKTDLPEEFSLRLAQGR"
     gene            2834..3331
                     /locus_tag="VKN64_00790"
     CDS             2834..3331
                     /locus_tag="VKN64_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58795.1"
                     /translation="MDKQGWHLKNWHFLGWLETVIKTAAIIIGIYVFFLGVKEPNFLL
                     IDNVPFIQLIIQLIILIILSLGLVIAIYNRYQNKDIVSMVFVLFNNWGHWGLIYGIFK
                     GVEARFLYSFAMIMLAGDLIKIIFLRKYNYSEGELAPKKFIYLTSVYIIGYILIILLG
                     LPTLL"
     gene            3417..4133
                     /gene="msrA"
                     /locus_tag="VKN64_00795"
     CDS             3417..4133
                     /gene="msrA"
                     /locus_tag="VKN64_00795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMA58796.1"
                     /translation="MNTFYILLISLLLVFTVSKVGMAAEETEKPPIDKMRPDNLKTAT
                     FAMGCFWGPDVVFGGVEGVWKTRVGYAGGDKENPTYHNLGGHTETIQIEYDPTAISYK
                     ELLEIFFENHNPYVKPYSTQYKSIVFYHNQNQKEIYQNYVEKLKENKTLYTQLKEYDK
                     FYYAEFYHQKYQLQGFSRLMKEVQKYYPKDMNFINSTLTARLNYFVGTREGKEMLEKE
                     KEYYGLDEETISWLKSFIKE"
     gene            4145..5935
                     /locus_tag="VKN64_00800"
     CDS             4145..5935
                     /locus_tag="VKN64_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA58797.1"
                     /translation="MKIKYLNGERLYYAFISGAKEVIENRKELNKINVFPVPDGDTGT
                     NLSRTLNMVMENVIADKSVKTTLNSMGDAALRGAIGNSGIIFAQFINGLKENLKAEKR
                     LNVDNFAEMIDNAVDYTYQAISEPVEGTMLTVMKDWSKSLEELRAKTDDFEELMSESL
                     KIARESLDNTKNTLEVLKKAHVVDSGALGFVYFLSGILEFIRTGKIQDINHYDIVLEN
                     EESTITEEEELEYRYCTEAYIEEVTKEIPQIREELSHLGDSLIIAGSSAKMRIHIHTN
                     EPAEFFAELNNYSTIIDQKVDDMLIQYNVKYDRISNIALVTDSIADLPKNYMDNNQIN
                     MIPINLVVDQTNYLDKVTVTSDNFYDLVDEAEEYPTSAQPSVKTVEDKLSFLADHYEG
                     IIVITVSSNLSGTYDSVQRAAENIQNGAKITIIDSKLDSGAQGLLVMEAAEKIKEGWD
                     YQEIVDYIENIKQNMYIYVSVDNFEYMVRGGRVSPLKGKLAGLLNLKPIVSLDEEGNG
                     IAFAKAFSKRGNNKKIKQIVKDHMEEDGIKRYSIVHGDGIERAQRYKKMFTQLIGKEP
                     DYIEDISPVIGLSAGRNSVAISLMTKKGDD"
     gene            5938..6708
                     /locus_tag="VKN64_00805"
     CDS             5938..6708
                     /locus_tag="VKN64_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMA58798.1"
                     /translation="MGSIFVQTLLFILAYFIVFFIIAVVKKNNSVVDIGWGLGFVLTS
                     YFVLINTGNINFRSIIVTLMVTIWGLRLSYHIYKRNKGKPEDFRYAKWREEWNNFYLR
                     SFFQIFVLQGILLFIIVSSVININSNPGAAFTVFDALGIVIWVVGFTFEALGDKQLKD
                     YINRPDSEKEGHIMKEGLWRYTRHPNYFGEATMWWGIFIIGLSIPNSWYFVISPITIT
                     YLLLYVSGVPLLEKRYADDEEFQEYAKHTNKFFPWFPK"
     gene            6733..7056
                     /locus_tag="VKN64_00810"
     CDS             6733..7056
                     /locus_tag="VKN64_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HMA58799.1"
                     /translation="MKVAKYLSLLGFIIMSAAIISGFIYGDFSGEGGIILSMIWGKVT
                     LIDIYIMFLIFSGWIYYREEQFIKFFGWFLLVLVFGAATACLYLYIAFERSKGDWNQF
                     FTGKR"
     gene            7177..7479
                     /locus_tag="VKN64_00815"
     CDS             7177..7479
                     /locus_tag="VKN64_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMA58800.1"
                     /translation="MTKTDLIDKVAEETGLTKKDSGEAINATIDTIMEYLKEEAKKPE
                     EKRDKVQLIGFGSFEVRDRSARKGRNPQTGEEITIPARKVPVFRVGKTFKEVVEDK"
     gene            7498..7968
                     /locus_tag="VKN64_00820"
     CDS             7498..7968
                     /locus_tag="VKN64_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMA58801.1"
                     /translation="MERDLEVTSTAFQDGDMLPEKYTADGADISPPLTIKNINPEGET
                     IAVIMDDPDAPVGTFTHWMIWNIPVDKNDIPEDVPKDKKVDSLADAVQGYNDFGEVGY
                     RGPAPPSGVHNYHFKIYILDQKIDIPFDSSKQKLVNTLKDNLLQKTEIVAKNER"
     gene            7987..8253
                     /locus_tag="VKN64_00825"
     CDS             7987..8253
                     /locus_tag="VKN64_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA58802.1"
                     /translation="MEVKVEQPNQEKLDELGVKNWPIWEKEVSEFDWSYDQKETCYIL
                     EGKAEVETPDGKVSFGKGDLVTFPAGLDCKWKILEDVKKHYILG"
     gene            8309..9052
                     /locus_tag="VKN64_00830"
     CDS             8309..9052
                     /locus_tag="VKN64_00830"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HMA58803.1"
                     /translation="MQIFQDAAKMIKNSQHITVFTGAGISVESGIPPFRGEDGLWEKY
                     DPYFLHINYFMNNPEESWKLNKKLFYSMFEDCQPNKAHQVVAQLEQEGYVNTVITQNI
                     DNLHQEAGSKKVYEFHGTYKELICTECGKIYEFQARYLKDLPPRCKSCNGILKPNYIF
                     FGEGIPQKAYQGSIKEAQEADLFIVIGTTGQVTPASLIPDMASRHGAKIIEVNTKRSN
                     FTNNITDVFLQGKATEMMEKLYNKLKEID"
     gene            9253..9834
                     /locus_tag="VKN64_00835"
     CDS             9253..9834
                     /locus_tag="VKN64_00835"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58804.1"
                     /translation="MKRLKDRNDGKKIYYAGIGSHSTPEDVLDIMVELGYLLAEENYI
                     LRSGGSPGADTAFEAGAKKADGQFEIYLPWDKFQDRSGDNYLDVRNLDNFNNAKRIAK
                     KHHPNFAELSESAKLLITRDTYQVLGKDLTTPSKFVVCWTPDGCITDRDRSKETGGTG
                     QAISIASRREIPIFNLKLKEHRRQVEEWINKLS"
     gene            complement(9894..>10148)
                     /locus_tag="VKN64_00840"
     CDS             complement(9894..>10148)
                     /locus_tag="VKN64_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA58805.1"
                     /translation="NKKTLQKMSEMIVDKIKEDKKAWVGILHGDRENTAQKLLEKTKS
                     ILPEDKEVEFFQNRISPTLGCHVGPSVYAFATLSGEILNI"
BASE COUNT         3508 a         1524 c         2046 g         3070 t
ORIGIN      
        1 tttattgaat tttttactat ttatgattta agaaaagaat ataacttatc ggctcaaagt
       61 agcaaggaat ggttaaatga cagggtaaaa tcttttttac tgactcttgt tctgacacat
      121 ctattttccc tgctattttt atttttaacc aataattatc cgcaagcctg gtggcttatt
      181 atgtcaataa tcctatcggt ttttgttgta ttattaaatt atctactgcc cgtcctaatt
      241 ttgccgctct tttatacttt aaaatcttat ccagaaggcg agctaaaaga cagactgatg
      301 tctaccttcg ataagttgga tattgaaatt gaagatattt atgagataaa tcttagttcc
      361 aaaatgactt ctgccaatgc ggccgtgatt gggatgggta aaacccgaaa gattctttta
      421 agtgataatc tggccgaccg gtttagtgcc gaggaaatag aggctgtttt gtctcatgaa
      481 atagggcatc atgttaataa tgatatttat aaaaatcttt tattactgcc ttttataatt
      541 gttataacag cttttatagt tcatctatcc tggcctacct tattgagctg gtttggatat
      601 gtaaatcaaa atttgattat cagcttacca ctgcttttat tatactgggg tattttatat
      661 tcaatcttta ctcccctcca actttatcta agtagaaaat atgaaagacg ggccgacaaa
      721 tttgcctttg aggtcatcga taagcctatg aatttaggga aagcttttgc caaattggct
      781 gatgacagtc tctctcgttt ggattataga tggtgggaga agttatataa agcctcacat
      841 ccttccataa aatcaaggat atatgaagct aaagaacata aagataataa cgattaatta
      901 aaatctctta aacaaaatta aatataggta taattttttg aaaattataa atcataggag
      961 tgtttattta tggaacataa aaccttatat tttgaagatg gggtattaaa gttaatagac
     1021 caaaggattt tacccctgga aacacagtat tatgaagctt ctgattacag ggaagtctgt
     1081 gaagctatca aaaaaatggt ggtaagggga gctcctgcca ttggagctac cggagcatat
     1141 ggatattacc tggcggtaag agagcacatc tctgctgaca atgtaatatt cttagatgag
     1201 attgaaaagg caaaagagga gcttttagct acccgtccca cagctgtaaa tttaagctgg
     1261 gggatcaggc ggatggagaa agttttaaag gaatgtcttg ctaaagatta taatgaggat
     1321 gaaatagaag aaagccttct gcaggaagct aataaaattg cgcaagaaga tattaaaacc
     1381 aataaaagat taggtaggtt tggtaaccag ttgatagaaa aaggagataa tatattaact
     1441 cattgtaatg caggtgccct ggctacagtt ggttacggta cagccttagg ggtaataagg
     1501 gctgcccatg cagaaggtaa aaacatcaag gtatatatgg atgaaacccg tccccgctta
     1561 cagggggcca gcctgactgc ctttgagatg ttagaagaag atatcccggc tactttaatc
     1621 gttgatagta cggccgctac cttaatgaaa aaaggtgtta ttgataaggt cgtggttggg
     1681 gcagatcgaa ttgctaaaaa tggagataca gccaataaga taggaacctt tatgttatcg
     1741 gtcttggcta aagaatatga tgttcccttt tacatagctg ctccgctttc taccgttgat
     1801 tttgagatag atagtggaga tgatatagaa atagagatca gagcagacaa agaagtgacc
     1861 gatatcagaa gtgttaggat tgccccggtg ggggtagatg tctataatcc tgcctttgat
     1921 gttacaccag ctgaaaacat taccgcgatt attactgaaa aaggagtaat agagcctcct
     1981 tttacagaaa atcttaataa ggctaggaag taatttccta acagctagtt ataatataat
     2041 caatatatga ggggttttta ttatggaaga tctaaattct attgccatta tttcagacat
     2101 tcatggaaat tacacggcat tggaaaatgt tttaaagaag attgagatgt atgatgaaca
     2161 gattaatgat ataatgtgtt taggagatct ggtcggatat aatccggaac cagaaaaatg
     2221 tgttaataaa ataagagaag tctgcagcaa ggttatcgcc ggcaaccatg ataaagcgat
     2281 ggtcggcgag cttaatatcc agtggtttag acagacggta aaaaaatcaa ttacctggac
     2341 aagagatcat ttaagtgaag aaagcaaatc ttacttaaag tctcttaata cgcgggaaga
     2401 ccttactatt aacggacgta aaattatctt agctcacggg agtcccgatc aaaattcacc
     2461 tttttcctat atatttaacc gttatgatat aaaagcgatt gaacccttga ttatggatac
     2521 taatatactt tttataggcc acacccatat tcctttttgt ttttgtaata agggagacgg
     2581 ctggcagcaa atcgaaaaag atgagattga aataaatccg gaatataatt atatagtaaa
     2641 tgttggtagt gtgggacagc cccgtgatag agatccccgc ttaagttttg tgatctttaa
     2701 tcccaataat aatttattaa cttttcatag atatgaatat aatattaaag aagtacagga
     2761 taaatttgct aagacagatt taccagaaga gttctcgtta agattagcgc aaggcagata
     2821 ggagaggata aagatggata aacagggttg gcatctaaaa aactggcatt ttctgggctg
     2881 gctggagacg gtcattaaaa cagccgctat tattattggt atttatgtat tttttctggg
     2941 agtaaaggag cctaattttt tactgattga taatgtacct tttatccaac ttatcatcca
     3001 acttatcatt ttaattattt tatccttagg cctagttatt gctatctata atcgttatca
     3061 gaacaaagat atcgtatcaa tggtttttgt attattcaat aactggggac attggggttt
     3121 aatatacggt atatttaaag gtgtagaagc aagattctta tattcctttg cgatgatcat
     3181 gttagcaggt gatctaatta aaattatatt tctgagaaaa tataattatt cggaaggaga
     3241 actcgcccct aagaaattta tttacctgac ctcggtttat attattggtt acatattaat
     3301 aattttatta ggtttaccaa ccttattata aatagtaagt attttactga attatccacc
     3361 gttatattat acgattagta tagtaaatat tacaaaacgt ataagaggtg gataaaatga
     3421 atacatttta tatattatta atttctttgt tattagtttt tacagtgagt aaggttggaa
     3481 tggcagcaga agaaaccgaa aagcctccta ttgataaaat gagaccggac aatctaaaga
     3541 cagcgacctt tgctatggga tgtttttggg gaccggatgt tgtatttggt ggtgttgaag
     3601 gggtctggaa aacaagggtt ggttatgcag gtggtgataa ggaaaacccc acctaccata
     3661 atctgggtgg tcatactgaa actatccaaa tcgaatatga tccaaccgca atttcatata
     3721 aagagctttt agagatattt tttgaaaacc ataatcctta tgtaaagccc tacagcactc
     3781 aatacaaatc gatagtattt tatcataatc aaaaccaaaa agaaatatac caaaattatg
     3841 tagaaaaatt aaaagaaaat aaaacattat atacccagtt aaaagaatat gataagtttt
     3901 attatgcaga gttttaccat caaaaatacc aactacaggg tttttcaaga ttgatgaaag
     3961 aagtacaaaa atattatccg aaagatatga actttattaa ttccacttta actgctcgtt
     4021 tgaattattt tgttggtacc agggaaggga aagaaatgtt agaaaaagaa aaagaatatt
     4081 atggattgga tgaagaaaca ataagttggc taaaatcatt tattaaagaa taaaggtggt
     4141 ttcgatgaag attaaatatt taaacggtga aaggctctat tatgccttca tttcaggtgc
     4201 aaaagaagta attgaaaata gaaaagaact taataaaatc aatgtctttc ctgtaccgga
     4261 tggagatacg ggtactaatc tctcgcgtac cttaaatatg gttatggaaa atgtgatagc
     4321 cgataaatcc gttaagacaa ccttaaattc aatgggggat gcggctttaa ggggagctat
     4381 cggtaactct gggattattt tcgcccagtt tatcaatggg ttaaaagaaa atttaaaggc
     4441 cgaaaagagg ttaaatgtag ataactttgc cgaaatgata gataatgcag tggattatac
     4501 ctatcaagct atcagcgaac cggtggaagg tacaatgctt acagtaatga aggattggtc
     4561 taaatcatta gaagaattaa gggctaaaac cgacgatttt gaggaattaa tgtccgaatc
     4621 cttaaagatc gctagagaat cgctggataa taccaaaaat accctggagg tattaaaaaa
     4681 ggcacatgtg gttgattccg gggcattagg ctttgtttac tttctttccg gtatcttaga
     4741 atttatcagg acaggtaaaa tacaggatat aaaccattat gatatagtgt tagaaaatga
     4801 agaatctaca attacagaag aagaagagct tgaatacaga tactgtaccg aagcttatat
     4861 agaagaggtc acaaaggaga tccctcaaat aagagaagaa ttaagtcatt tgggagactc
     4921 tttgataatt gccggtagtt cagccaaaat gaggatccat atccacacca atgaaccggc
     4981 tgaattcttt gcagaactta ataattacag tacaataatt gatcaaaaag tggatgatat
     5041 gcttattcaa tacaatgtaa agtatgaccg tatatctaat atcgcccttg taaccgattc
     5101 aattgcagat ttaccgaaaa attatatgga taataatcag attaatatga taccgataaa
     5161 tttagtagta gaccaaacta attatctgga taaagtaaca gttacctccg ataacttcta
     5221 tgatctggta gatgaggcag aggagtatcc tacctcagcc caaccctctg tgaaaacggt
     5281 cgaagataaa ctatcctttt tagctgatca ttatgagggc ataatagtga taacggtttc
     5341 cagtaatttg agtggaactt atgattctgt acagagggca gccgagaata tacaaaatgg
     5401 tgccaagata acaataattg attcaaaact tgattctggt gcccagggac ttttagttat
     5461 ggaagcggct gaaaagatta aagaaggatg ggattatcaa gagatagttg attatataga
     5521 aaatattaag caaaatatgt atatctatgt tagtgtagat aactttgaat acatggtaag
     5581 aggtggtcga gttagtcccc ttaagggtaa attagccggc ctattaaacc ttaaaccgat
     5641 cgtttcgctt gatgaagaag gcaatggtat agcctttgct aaagccttta gtaaacgagg
     5701 caacaacaaa aagatcaaac agattgtaaa ggaccatatg gaagaggatg gaataaagag
     5761 atattctatt gtacatggag atggtattga gagagcccaa cgatataaaa agatgttcac
     5821 ccaacttata ggaaaagaac ctgattatat tgaagacata tctcctgtca taggactaag
     5881 tgccggccgc aacagtgtag ctatatcatt aatgacaaaa aaaggagatg attagtaatg
     5941 ggttcaattt tcgtgcaaac tttattgttt atcttggcat actttattgt gtttttcata
     6001 atagcggttg tcaaaaagaa taactcggtt gttgatattg gttggggact tggttttgtc
     6061 ttaacctcct actttgtatt aataaatacc ggtaatatta attttaggtc cattattgta
     6121 actttaatgg ttacaatttg gggacttaga ttatcatacc atatctacaa aagaaataag
     6181 ggtaaaccag aagattttcg ttacgcaaag tggagggaag aatggaataa cttttattta
     6241 aggtcttttt tccagatatt tgtcttacag gggatacttt tattcataat tgtatcttca
     6301 gttattaata tcaactccaa tccaggggca gcctttacag tattcgatgc tttaggtatt
     6361 gtgatctggg tagttggctt tacctttgaa gcactcggtg acaaacagct taaggactat
     6421 attaatagac cagactcaga aaaggaaggc catattatga aagagggact ctggcgttat
     6481 accagacacc ctaactattt cggtgaagcc actatgtggt gggggatttt catcataggt
     6541 ctttctatac caaacagctg gtactttgtg atcagtccga ttacaataac ttatctactg
     6601 ctttatgttt ctggggtacc tcttttagag aaaaggtatg cagatgatga agaatttcaa
     6661 gaatatgcta agcataccaa taagtttttc ccctggtttc ctaaataaat agcaagaatg
     6721 gaagtgtaaa caatgaaggt agccaaatat ctatctcttt taggttttat tatcatgtca
     6781 gcagctatca taagcggatt tatatatggg gatttttccg gtgaaggcgg tattattctt
     6841 tcgatgatct ggggtaaggt gactttaatt gatatttata tcatgttttt aatattttca
     6901 ggctggatct attatcgaga ggaacaattc attaaatttt tcggttggtt tttactggtt
     6961 ttagtattcg gagccgcaac agcctgtctt tacttatata tagcatttga gaggagtaaa
     7021 ggtgattgga accagttttt tactggaaaa aggtagaaaa atatataaaa acgttgtaat
     7081 ttcaggaata aaagtgataa aaaagaagga aatttctaac ttttgtataa attaaataca
     7141 ataagaataa aacttaaatt aaggggttga gtattgatga caaaaacaga tttaattgac
     7201 aaggttgctg aagaaactgg tttaactaaa aaggactctg gcgaggctat taacgctact
     7261 attgacacaa tcatggaata tttaaaagaa gaagctaaga aaccagagga aaaaagagac
     7321 aaagtacaac ttattggatt cggctcattt gaagtaagag acagaagtgc ccgtaagggt
     7381 agaaatcctc aaactggcga agaaattact attcctgcca gaaaagtacc tgtatttaga
     7441 gtaggcaaaa cctttaaaga agtagtggaa gacaagtaaa acaaagaggt gcataatatg
     7501 gaaagggatt tagaagttac ctcaactgct tttcaggatg gagacatgct gccggagaaa
     7561 tacacagcag atggagctga catttctccc ccacttacaa ttaaaaatat taatcctgaa
     7621 ggtgagacta tagcagtaat aatggatgat cccgatgcac cagttggaac tttcactcac
     7681 tggatgattt ggaatatacc agttgataaa aatgatattc cagaagatgt tccaaaagat
     7741 aaaaaggtag attccctggc tgatgcagtt cagggctata atgatttcgg agaagtaggt
     7801 tatagaggac ctgctcctcc cagtggagtt cataattatc attttaaaat ttatatctta
     7861 gatcaaaaaa tagatattcc atttgattca agcaagcaga aattagtgaa tactttaaaa
     7921 gataacctct tacaaaaaac agaaattgtc gcaaaaaatg agaggtaaaa aagaggtata
     7981 aagaaaatgg aagtaaaagt agaacagcct aatcaagaaa aattagacga attaggtgtg
     8041 aaaaattggc ctatttggga aaaggaagtt tcagaatttg attggtctta tgaccaaaaa
     8101 gaaacttgtt atattctaga aggcaaggca gaagttgaaa ctccagatgg aaaagtaagt
     8161 ttcggtaaag gagacttggt tacttttcca gccggtctgg attgtaagtg gaaaatatta
     8221 gaagatgtta aaaaacatta tatattaggt taaggattta tcaagaagga ggcggctgcc
     8281 tccttctttt ctcatgggag gtgtaatttt gcaaattttt caagatgcgg caaaaatgat
     8341 taagaattca cagcacatta ccgtctttac aggagctggt atatcggttg agagtggtat
     8401 acctcctttt cggggagaag atggactctg ggaaaagtat gacccctatt ttctccacat
     8461 caattacttt atgaacaatc ccgaagagtc ctggaagtta aataaaaaac tattttacag
     8521 tatgtttgaa gactgccaac cgaataaggc acatcaagta gtggcccaac tagaacaaga
     8581 gggatatgtc aacacagtca taactcaaaa tattgataat ttacaccagg aagccggcag
     8641 taaaaaggta tatgaatttc acggaaccta taaagaatta atctgtacag aatgtggtaa
     8701 gatctatgag tttcaagcca gatatctaaa agatcttccg cctcgatgta agagctgtaa
     8761 tgggatatta aaacccaatt acatcttttt tggagagggc atacctcaga aagcctatca
     8821 gggatctata aaagaggccc aggaagcaga tttgtttata gtaataggaa ctaccggtca
     8881 agtaactcct gcctctctaa tacctgatat ggcctcccga catggggcaa agataattga
     8941 agtaaatacg aagaggtcaa actttactaa caacattacg gatgtatttc tacaggggaa
     9001 agctaccgaa atgatggaaa aactttataa taaattaaaa gagatagatt aatttaattt
     9061 ttcatttttt tcttaaagtt tattaagttc gcactacatt tatcacaata cagagaacta
     9121 gtccagttat tagaaccaca acatggacaa attatttttt tactcatgaa taacattcct
     9181 cctattaaaa atcagcacac tcttatgttt ttaccatata ttagtcattt tgtaaaggag
     9241 attttaaaaa tcatgaagag attaaaagat agaaatgatg gaaaaaaaat atactatgcg
     9301 gggatcggca gccacagtac gcccgaagat gtcttggata ttatggtgga gttaggttac
     9361 cttttggctg aagagaacta tattctccgt tcaggcgggt caccgggggc agatactgct
     9421 tttgaagccg gagctaaaaa ggcagacggg caatttgaaa tctacttgcc ctgggataaa
     9481 tttcaggacc gcagtggtga taattatcta gatgtaagaa acttagataa tttcaataat
     9541 gctaaaagaa tagctaaaaa acatcatccc aactttgcag agctatctga gagtgccaag
     9601 ttattgatca ctagagatac ctatcaggtc ttaggaaaag accttacaac accttcaaag
     9661 tttgtggtgt gctggactcc agatggctgt attacagacc gggatagaag taaagagacc
     9721 ggtgggaccg gccaggcgat atcaattgcc agtcggagag agattcctat ctttaattta
     9781 aagttaaaag agcatagaag acaggttgag gaatggatca ataagctttc ttaacctctt
     9841 aaataaaaaa accattatct ggtctcagat aatggtttca ttgatttcaa gttttaaata
     9901 tttaatattt cgcctgataa agtagcaaaa gcataaacag aaggtcccac atgacagcct
     9961 aaggtgggag atatccgatt ttgaaagaat tctacttctt tatcctcggg taatatagat
    10021 ttggtttttt ccagtaattt ttgggctgta ttttctctat caccatgtaa gatgccgacc
    10081 caggctttct tatcttcttt aattttatcg actatcattt cgctcatctt ttgtaaggtc
    10141 tttttatt
//
LOCUS       DATGCJ010000019         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_270354, whole genome shotgun sequence.
ACCESSION   DATGCJ010000019 DATGCJ010000000
VERSION     DATGCJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270354"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="VKN64_00845"
     CDS             <1..1687
                     /locus_tag="VKN64_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA58806.1"
                     /translation="ISGKENEDKIRDFIKKVQKQKEMERTSPESEKEGIFTGSYATNP
                     MTGQEIPILIANYVLMGYGTGAIMAVPAHDQRDFDFAKKYDLPIKAVIQPDDLEEKLD
                     GSTMEEAYPEEGHLINSGKYNGMDVEEAFAAMADDMEAEGIGERQINYRLRDWLISRQ
                     RYWGAPIPIVYCDECGAVPVPEEDLPVLLPDNVEFSATGFSPLAKDEDFVNTTCPKCG
                     GEAKREIDTMDTFVDSSWYFLRYIDPHNDSLPVDYDKAEDWFPVDQYIGGIEHAILHL
                     LYARFFTKVIRDLDLISAGEPFTKWLAQGMVLKDGDKMSKSKGNVVDPGRILDEYGAD
                     TARLFILFASPPEKDLEWSEEGVEGADRFLNRVWRFIANNIEEIKEVDVSEVTIDQSS
                     LNDEETELHRKLHETIQKVTEDIEDRLNFNTAISAIMELVNEIYHYLGNNSDKINKEL
                     FAFITEKVLLMLAPFAPHMTEELWHRIGRENSIHRAQWPAFDAEAAKKEEITIVIQIN
                     GKVRDRIDVSADISEEKLKEKVFAQEKVKKYTEGKEIVKTIVIPKKLVNVVVK"
     gene            1775..1984
                     /locus_tag="VKN64_00850"
     CDS             1775..1984
                     /locus_tag="VKN64_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMA58807.1"
                     /translation="MGREFKCEKCGSKSYDKTELRTTGGKLSKLFDVQNKKFIAVSCS
                     NCGYTELYRINDSGVLSNIFDFLTN"
     gene            2002..2832
                     /locus_tag="VKN64_00855"
     CDS             2002..2832
                     /locus_tag="VKN64_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA58808.1"
                     /translation="MSGIVFKKTKDLEQISNFYQNVIGMELWQDQDKCKIFEKGNLQL
                     GFCEGDEIDNDGIITFYYNNKKEVDKLYNNGKLEVIEEPKENDKFNIYQFFAKDPEGR
                     TLEFQTFLHNINPFLTGKELLLERRSYREYSEKEIPEEVINKVIDISRYAPTSMNSQS
                     YYFKFIRDEELICDLASIRKTASEPIKKAPMAVAICSDNEQSNRYQQDADIAAYHFML
                     AARLYNLGTCWIADMDRESIKRKLDIPVDHYIATITPLGYIKDEKDAPSRKDPSKFIR
                     "
     gene            2853..3305
                     /locus_tag="VKN64_00860"
     CDS             2853..3305
                     /locus_tag="VKN64_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HMA58809.1"
                     /translation="MREKACVVDVTGDTAKVVVTRHSACSKCNKDCILGMEDSHEQDE
                     MYVEVKSKKNVEVGDTVSIELKDSSLVLGSLMIYLIPLLFFVGGYFIGARLSWLFGTL
                     SAEVTGILSSFVFLYLSYRLTKKIDKRISKSEKFQSEIVNIVSKNENI"
     gene            3323..3595
                     /locus_tag="VKN64_00865"
     CDS             3323..3595
                     /locus_tag="VKN64_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HMA58810.1"
                     /translation="MNSISEEVKEDLLVRLNRIEGQTRGVKKMVENDKYCVDILTQIV
                     AARGALKKVGLKVLQEHINGCVQSSLQNEDEEIIDELLNVINRFMD"
     gene            3878..4510
                     /locus_tag="VKN64_00870"
     CDS             3878..4510
                     /locus_tag="VKN64_00870"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HMA58811.1"
                     /translation="MQHLEYFKVPKILFADDKLNLNEFKVLVKLFSKIDQSSLITKNK
                     WLLEKNNLNIKKEIINSLLKKNWLNKEEKQLYLNIPESVLPKDKEEVNYEKAGSKIAS
                     KWDEYFGTLNLTPTLLQKLLSFMEDGISEDVIIEVMKISSKKAEGNPANYMISILRDY
                     LNRSIYTIYDFKEEKQRMEEYEKRLQEINRKKERRDEKEALESDFEDRYR"
BASE COUNT         1708 a          648 c          946 g         1265 t
ORIGIN      
        1 gatttccggt aaagaaaatg aagataaaat tcgagatttt attaaaaagg tacagaaaca
       61 aaaagagatg gaaagaacct cacctgaatc ggaaaaagaa ggtatattta cgggtagtta
      121 tgctacaaac ccgatgaccg gtcaagaaat cccaattttg atcgctaact atgttcttat
      181 gggctatggt acaggtgcga taatggctgt acccgcccat gaccaaagag actttgactt
      241 tgctaaaaaa tatgatcttc cgataaaagc tgttatccag cccgatgatc ttgaagaaaa
      301 attggatggt tctaccatgg aagaagctta tccagaagaa ggtcatctca ttaattcggg
      361 taaatataac ggcatggatg tagaggaagc ctttgcagca atggctgatg acatggaagc
      421 agagggaatc ggcgaaagac agatcaacta taggttgaga gactggttga tttcaaggca
      481 gagatactgg ggagctccta tcccgattgt ctattgtgat gaatgtgggg ctgtaccagt
      541 accggaagag gacctacctg tcttactacc ggataatgtg gaattttctg caaccggttt
      601 ttctcccctg gcaaaagatg aagactttgt aaatactacc tgtcccaagt gtggcggaga
      661 ggcaaaaaga gagatcgata ctatggatac ctttgtagat tcatcctggt actttttgag
      721 atatatcgat cctcataatg atagtctacc ggttgattat gataaagcag aagactggtt
      781 cccggttgat caatatattg ggggaattga acatgcgatt ctccacttgc tgtatgcaag
      841 attcttcacc aaagtaataa gagatcttga cctgattagt gctggtgaac cctttactaa
      901 atggttagcc cagggtatgg tgctaaaaga tggagacaag atgtctaaat caaaaggtaa
      961 tgtagtagat ccaggccgga tacttgatga atatggagca gatacagccc gcctctttat
     1021 cctgttcgct tccccaccgg aaaaagatct ggaatggagt gaagaagggg tcgaaggtgc
     1081 tgaccgtttc ttgaataggg tctggagatt tattgcaaat aatattgaag aaattaaaga
     1141 agtagatgtt agtgaagtta ctatagacca atctagttta aatgatgaag aaacagaact
     1201 acaccgtaaa ctacatgaaa caattcagaa ggtaacagaa gatattgaag atagacttaa
     1261 ctttaataca gctatcagtg ccattatgga acttgtcaat gaaatttacc attatttagg
     1321 gaacaatagt gataagatca ataaagaact atttgccttt atcacggaaa aagtactgct
     1381 tatgctggct ccctttgccc ctcatatgac agaggaactt tggcatagaa ttgggcgaga
     1441 gaatagtatc caccgtgcgc agtggccggc gtttgatgct gaggcagcta aaaaagaaga
     1501 gatcaccatt gtaattcaaa tcaatggtaa agtaagagat agaatagatg tgagtgctga
     1561 tatttccgaa gaaaaactaa aagaaaaagt atttgcacaa gaaaaagtta aaaaatatac
     1621 agagggcaaa gaaatagtaa aaacaattgt aatacccaaa aagcttgtaa atgtagttgt
     1681 caaataacca aaaaataata attaactaaa acccctgttt taatgacagg ggttttttga
     1741 tataataaag aaaatagtta atgggggttg gcagatgggc agagaattca aatgtgaaaa
     1801 atgtggaagc aaaagttatg ataaaacaga gttgcggacc acaggaggaa aactatctaa
     1861 actgtttgat gtccaaaaca aaaagtttat cgcagtaagc tgcagtaact gcggttatac
     1921 agaactttat agaataaatg acagcggtgt cttgagtaat atttttgatt ttttaaccaa
     1981 ttaatttagg gagtgttatt tatgtcaggt atcgtattta aaaaaacaaa agatttagaa
     2041 cagatatcta atttttatca aaatgtcata ggcatggaac tatggcagga ccaggacaaa
     2101 tgcaagattt ttgaaaaggg caatttacaa ctaggatttt gtgaaggtga tgagatagat
     2161 aatgatggaa ttattacctt ttattataac aacaaaaagg aagtagacaa gttatataat
     2221 aacgggaaac ttgaagtaat tgaagaacct aaagaaaatg ataaatttaa tatataccag
     2281 ttttttgcaa aagatccaga agggcgtacc ctggagtttc aaactttctt acataatatt
     2341 aaccccttct taaccggcaa agaactgtta ttagaaagaa gaagttatcg tgagtatagt
     2401 gaaaaggaaa ttccggaaga agtaataaac aaagtaattg atattagtag atatgcccct
     2461 acctctatga actcacagtc ttattatttc aagttcatca gagatgagga attaatctgt
     2521 gatcttgctt ctatcagaaa aaccgcttca gaaccgataa aaaaagctcc gatggcagta
     2581 gcgatttgtt ctgataatga gcagtctaac agatatcaac aggatgcgga tatagcagct
     2641 tatcatttta tgttagcggc cagattatat aacctgggga cctgctggat tgcagatatg
     2701 gaccgggaaa gtataaagag gaagttggat atacctgtag atcattatat tgcgacaatt
     2761 acccctttag gttatataaa agatgaaaag gatgcgccca gtaggaagga cccgtctaaa
     2821 ttcattagat aattagacta ggagtgttaa agatgagaga aaaagcttgt gtggtagatg
     2881 tgacaggtga tacggctaag gtggtagtaa cccgccattc tgcctgtagt aaatgcaata
     2941 aagactgtat tttggggatg gaagattcac atgaacagga tgaaatgtat gtagaagtaa
     3001 aaagcaagaa gaatgtagag gtcggagata cagtaagtat tgaattaaag gacagttccc
     3061 ttgtactcgg atctttaatg atatatttaa ttcccctctt attttttgta ggtggttact
     3121 tcattggggc ccgcttaagt tggctctttg gtactttgag tgctgaagta acgggaattt
     3181 tatcttcttt tgtcttttta tacctctctt atcgtctgac aaaaaagatc gataagcgga
     3241 tttccaaaag tgaaaaattc caatcggaaa ttgttaatat tgtttccaaa aatgaaaata
     3301 tctaattagg atgtgatcac agatgaattc aatatctgaa gaagtaaaag aagacctact
     3361 tgttcgctta aataggatag aaggccagac ccggggagtc aaaaagatgg tcgaaaatga
     3421 taaatactgt gtagatatct tgactcaaat cgtggcagct cggggtgctt taaaaaaagt
     3481 aggcttaaaa gtattacagg aacatattaa tggttgtgtc cagagttcat tacaaaatga
     3541 ggacgaagag ataatagatg agcttttaaa tgtaataaat agattcatgg actaatattt
     3601 tggtaaatta tgtttatgaa ataaggggtt ttgcagataa agataaaaaa ataaaaaaaa
     3661 ttttaaaaaa caaaatccct ataatttaag taataccgga ccaaaacaaa caggagttca
     3721 cctcctgttt tttaatttta aaaaagggaa aaatgaactc ttgttgaatt taaaagatac
     3781 gcaaaaaaat aataaaaaaa taaaattatt gtgattgaat taaaaaatat attatgttat
     3841 gattattgat gctgccaaca agtcaggagt gattaaaatg cagcatttag agtatttcaa
     3901 ggttccgaaa atacttttcg cagatgataa attaaatcta aatgaattta aagttttagt
     3961 aaagttgttt tcaaaaattg atcagtctag tttgattaca aaaaataaat ggctgcttga
     4021 aaaaaacaat ttaaatataa aaaaagagat aattaattcc ttacttaaaa aaaattggtt
     4081 aaataaggaa gaaaagcagc tgtatttgaa cattcccgaa agtgttttac ccaaagataa
     4141 agaagaagta aattatgaaa aagcgggttc caagatcgcc agtaaatggg atgaatactt
     4201 tggaacctta aatttaactc ctactctttt acaaaaactc ctctccttta tggaagacgg
     4261 tattagtgaa gatgtgataa tcgaagtaat gaaaatcagt tcaaaaaaag ccgaaggcaa
     4321 tcctgccaat tatatgattt ctatcttaag agactatcta aatagatcaa tatatactat
     4381 ctacgacttt aaagaagaaa aacaaaggat ggaagaatat gaaaaaagac ttcaagaaat
     4441 taatcgaaaa aaagaaagaa gagatgaaaa agaagccctc gaatcagact tcgaagaccg
     4501 atatagataa gcatgtccct aaagaagaag cagttaagaa gatctatgat gtgataataa
     4561 ataacag
//
LOCUS       DATGCJ010000020         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_270381, whole genome shotgun sequence.
ACCESSION   DATGCJ010000020 DATGCJ010000000
VERSION     DATGCJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270381"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VKN64_00875"
     CDS             <1..220
                     /locus_tag="VKN64_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58812.1"
                     /translation="KYLRELDRKSPNKIMCAADFMGVKPQLLEARREELYNRFPVNQD
                     WHDRYKEGKVTTQYYLAEARKELQDFIY"
     gene            243..806
                     /locus_tag="VKN64_00880"
     CDS             243..806
                     /locus_tag="VKN64_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMA58813.1"
                     /translation="MLIDLSIEVTPELREQALKNEEKTVGGHIGTHFDVMDKEFPLEF
                     IKRKGIVFNISNSKEREIKVKDIDLDQIKENMFVGFYSGFLKEVGYGTEQYFYEHPQL
                     SKDLIDKLLAKKISIIGIDFAGIRRREEHSEMDQYCADQGVFIVENLNNFDKLLKDKK
                     SVRFKAYTFPIKYSGMSGLPCRVVAAV"
     gene            834..1619
                     /locus_tag="VKN64_00885"
     CDS             834..1619
                     /locus_tag="VKN64_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HMA58814.1"
                     /translation="MKIYQVDAFTDQPFKGNPAAVCILDKPKEANWMKNVAGEMSLPE
                     TAFLLKEKTGYNLRWFTPTSEVDLCGHATLASAHVLWETERIDKMRDIEFNTKSGLLI
                     ARNKDGWIELDFPIEAPKEEFAPQILINALDVEPVYVGKNRFDYIIEVNSEEIVKNLD
                     PDFRLLREVETRGVIVTSKSTSNKYDFISRFFAPSIGIDEDPVTGSAHCCLAPYWAEK
                     LNKNEFLAYQASDRGGSIKIRLGEERVYLSGQATMVLKGELME"
     gene            1616..2083
                     /locus_tag="VKN64_00890"
     CDS             1616..2083
                     /locus_tag="VKN64_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMA58815.1"
                     /translation="MKSWTKKEIKMSDIRYIVNVEAVIYRDDQYLLIKRSDKEEHAPG
                     ILSNAGGKVEIKNPQKEVLEKTIIREIKEEVDIELKSPFHYLESKSFYLDKKELIIDI
                     IFLCEYKSGQAKCKDKEEVDQVYWLSYKEIMANKEIEDWLKNTIAKAEKFRKK"
     gene            2098..>2676
                     /locus_tag="VKN64_00895"
     CDS             2098..>2676
                     /locus_tag="VKN64_00895"
                     /inference="COORDINATES: protein motif:HMM:NF040149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HMA58816.1"
                     /translation="MSLNLITDEVEILIDILKRKDNNKPIKKVQWDELFNSNGFKILT
                     VREKEMNQGIDKFEFKDFVMNKIASDRYISYKKSLEKLKSMNLDDIINRTRDYLPDNT
                     RLDTDIVPVIKPQKNSFIHRINDKLILFLYLEPDISKEKLENKLIHELHHIGLDYVYQ
                     NSDYSCLSKPAQKVIEWTNAFGEGFAMLAAAKG"
BASE COUNT         1000 a          347 c          561 g          768 t
ORIGIN      
        1 aaaatattta agagaacttg ataggaaatc acctaacaag ataatgtgtg ctgctgattt
       61 catgggagtt aaaccccagc tcttagaggc aaggcgagaa gagttgtata accggtttcc
      121 tgtaaaccaa gattggcatg atagatataa agagggtaaa gtaacgactc aatactatct
      181 agcagaagcg agaaaagagt tacaggattt tatatattaa aaacatatta gaggtggaaa
      241 aaatgctaat tgaccttagt atagaggtaa cgccagaatt aagagaacaa gctctaaaaa
      301 atgaagaaaa aactgttggg ggacatattg gtactcattt tgatgtcatg gataaagaat
      361 tccccttaga atttataaaa agaaagggga ttgtctttaa tataagtaat agcaaagaaa
      421 gagaaattaa agttaaggat attgatttag atcaaattaa agaaaatatg tttgtaggat
      481 tctattccgg tttcttaaaa gaagttggtt atggtacaga acaatatttt tacgaacatc
      541 cccagttatc aaaagattta attgataagt tgcttgctaa aaaaatatct attattggga
      601 ttgattttgc cggtatcagg cgaagggagg agcattcaga gatggatcag tactgtgctg
      661 accagggagt ttttattgtg gaaaatctaa ataactttga taagttacta aaagataaga
      721 aatctgttcg gtttaaagcc tatacctttc caattaaata ttctgggatg tccggtctac
      781 cctgtcgtgt ggtggcagcc gtataagaat atattatttt tgaggaggta aaaatgaaaa
      841 tatatcaagt agatgctttt acagaccaac cctttaaggg taatccagct gccgtttgta
      901 ttttagataa gcctaaagaa gccaactgga tgaagaatgt agcaggagag atgagtttgc
      961 ctgaaacagc ttttctctta aaggaaaaga ctggatataa cttgcggtgg tttaccccga
     1021 caagtgaagt tgacctctgc ggtcatgcta ctttggcgag cgcccatgtt ctctgggaaa
     1081 cagagagaat tgataaaatg cgagatattg agtttaatac taagagcggt ctcctaatag
     1141 caagaaacaa agatgggtgg attgaattag actttccgat tgaagcgcct aaagaagaat
     1201 ttgcacctca gatacttata aatgcacttg atgtagagcc ggtctatgtt ggcaaaaatc
     1261 gttttgatta catcattgaa gttaattcag aagaaattgt caaaaattta gatccagatt
     1321 ttagattgtt aagagaagtt gagacccggg gagtgatagt aacaagcaaa tcaacctcta
     1381 ataaatatga ctttatctct aggttttttg ctccttcaat cggcatagat gaagatccgg
     1441 taactggttc tgcccactgt tgtttagcac cctattgggc agagaagtta aataaaaatg
     1501 agtttttagc ttatcaggct tctgacagag ggggaagtat taagatcaga ttaggagaag
     1561 agcgggtata tctaagcggg caggctacta tggttttaaa aggagaattg atggaatgaa
     1621 aagttggacc aaaaaggaga ttaagatgtc agatattaga tacattgtta atgtggaagc
     1681 ggttatctat cgagatgatc aatacttgct tataaaaaga agtgataagg aagaacatgc
     1741 acccggtata ttatcaaatg ccggcggcaa ggttgagatt aaaaatcctc aaaaagaagt
     1801 actggaaaaa accataatta gagagatcaa agaagaagta gatattgagt taaaaagccc
     1861 ttttcattat ctggagagca agtcttttta tctcgataaa aaagaattaa taattgatat
     1921 tatcttccta tgtgaatata agtcggggca agccaaatgt aaggataaag aagaagttga
     1981 tcaggtttac tggttgtcct ataaggaaat tatggcgaac aaagaaatag aagattggct
     2041 gaaaaatact atcgccaaag cagagaaatt cagaaaaaaa taatctgggg tgaaactgtg
     2101 agccttaatt tgattactga tgaagtggaa atattgatag atatcttaaa aagaaaagat
     2161 aataataaac caattaaaaa agtacaatgg gatgaattat ttaatagtaa tgggtttaaa
     2221 atactaaccg taagagaaaa agaaatgaat caaggaatag ataaatttga attcaaagat
     2281 tttgtaatga ataaaatagc ttcagataga tacatttcat ataaaaaatc acttgaaaaa
     2341 ttaaaaagta tgaatttaga tgatattata aatagaacaa gagattattt gcctgataat
     2401 actagactag atacagatat tgttccggtc attaaacctc aaaagaatag ttttattcat
     2461 cggattaatg ataaattaat tctttttctt tatcttgaac cagatatttc aaaagagaag
     2521 ttggaaaata aattgataca cgaactacac catatcggct tggattatgt ctatcaaaat
     2581 agtgattata gctgtctatc aaaaccagct cagaaagtta tagaatggac aaatgccttt
     2641 ggtgaaggct ttgccatgtt agctgctgct aaaggg
//
LOCUS       DATGCJ010000021        20885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_404798, whole genome shotgun sequence.
ACCESSION   DATGCJ010000021 DATGCJ010000000
VERSION     DATGCJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404798"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VKN64_00900"
     CDS             complement(<1..573)
                     /locus_tag="VKN64_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA58817.1"
                     /translation="MIWQITIQKLPLSEDFDNVIQIKRNYYINHYVLGALASEVTETD
                     KIGFVGGLKISWTIGVVNAIQQAVQEHNPQVEFEYIYVGSFNNPLKTKQATETLINRK
                     VDIIMSGVNLGNYGMFRAVEDANRPTYVTSIYTDKTELAPDHFLTSDLFKYNSLIIEI
                     VNGIINEDNTTGLYNMEYGPDQGRYLKLPVN"
     gene            complement(620..892)
                     /locus_tag="VKN64_00905"
     CDS             complement(620..892)
                     /locus_tag="VKN64_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58818.1"
                     /translation="MNININNKLVTAVILLLVLTLFPLGNITEAQEAEYKMAAVFPGS
                     IQDADWNTIGYKALQNVNDELGLEVSYLEQVAVPDAPRVVREYISL"
     gene            1072..2250
                     /gene="rlmD"
                     /locus_tag="VKN64_00910"
     CDS             1072..2250
                     /gene="rlmD"
                     /locus_tag="VKN64_00910"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMA58819.1"
                     /translation="MKSKDKKGVKICKHYDLCGGCSSLDVPYQKQLEQKKAKVKNYFL
                     ENDIMEFPLSEVIESPKIYEYRNHMELSFGDLEIGGKLQLGLHPRGKRYEVVTIDGCY
                     LIDEDFRKIIKTMIDYFRQTNLKKYHVKLREGYLRNVKLRKGINTGEILVNLITNSQR
                     DPDLSDLFEELKDLDYKDELVGILHTINDDYADAVKADEVRTYYGRDYFYEKLLGYKF
                     KVSPLDFFQVNTKAAEKLYKIVIEYFKGSKEQKVYDLYSGTGSISQVISPHIKKVIGI
                     ELDESAVKKARANLKLNNVNNCEFIQGDVREVLKNKKEPVNKVVVDPPRPGLHSEVTR
                     SIIEISPQEIIYVSCNPQTQAQDLKKLLYNDYHIEDIKLVDMFPHTTHIESVAHIVKD
                     "
     gene            complement(2254..4113)
                     /locus_tag="VKN64_00915"
     CDS             complement(2254..4113)
                     /locus_tag="VKN64_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58820.1"
                     /translation="MIEQTLLGKTLLRKNLITKRQLNNAYDIHTDTNERLSKILIRNK
                     MVEGLEIYKILADLSENYISNQLSEKTIPLKEEIDYGFVKKFNPTFLNEKLFMPLYFK
                     NNIIKIIVFDFKDKSIDRFLKNKFGDILIEKVELYPKEIKYFIEYAFKDEIIKKQKNN
                     IKRKNNETALNVFTRGQLLGVAIFGILLITGLFFYFNSTVDILIYTLNTLFLLFLISR
                     FIIYLAKKNKIDKEGASKEKNIRDQDSLPVYTVLIPVTQSTSDLNNLILSLKKMDYPS
                     EKMEVIFLVDKKLKDKIKNLALNNESWLIYTIPASKLRTEIEIYNFGLNLASGKYITI
                     YKGSTIPEADQLKKSVFKFENSQRNYFCLQSPLEAAGSNNSLFSKYLSIENNLKYKFF
                     SYLDEGIYHYKTAAIRQFGGWDLYNTAENLEIIRKAAARGYEIGIINSKTFQLSSDNF
                     NNWFFNFTNSLKGYMQTMLVYNRKPISKLKEDGLKDILFNNLTIGGELLLPFLYTCLI
                     LISAVFLAAGRIMTFLTWQPQFLFTVFNLVLFLIFNTYYIFSNDLKIKYLDRILITPL
                     LPVHWVIKSYAFIKSIMLLIKRPHYRLNRAHDMQEDQIMFMQINFLEKIKETN"
     gene            complement(4130..6715)
                     /locus_tag="VKN64_00920"
     CDS             complement(4130..6715)
                     /locus_tag="VKN64_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HMA58821.1"
                     /translation="MIEIKFNSFLITIFILLLTTTAVGAASYTIQTGYFSKENNALNL
                     SQKLSEQGFETYKYQQNNRYYVFVGQFNNYNSAADTLAEVKNIVPEAYIRKVDFEFTK
                     SRTPSEDNKKQPESETKEQTAQKSKEVKEDEIAAEDKDDINKENKKNKDQVEDNKKEI
                     SNSEEVSTEEDTDQVEKPSVKKDLDYYKENNQFEIKTAFLVQKTENNSQFLFPNGEEV
                     KYSTDSYNDKIKLNNNYHLVLRDNELIDIFQQKNNNEIIYTLKDFGYKNDIILKGWQN
                     SFIINIPLSNKVIEDQIYLKLNLITSQFIGELSNIDIMINNQPVKKYNFKSGQNQYSL
                     IFPLKLKNINKLQIEIKAQLRNKQKDYTKSLYENMWIKIDNDSGIGFKLKDNQNYQIN
                     NFLGIEAEKFNLYYEGKLSRQEFHDYIKLYTYLNRLLKDLPPEIELKLSSEDVEFNNL
                     DNRIRNIVISEEYDKTSLDEFMTLHLSSEDIDFLISDFLNLVNANFLDVTEYNLKQDV
                     NYKLSSYQTDFLEANRKGWGEINYHYHLPINIFTNWPRDFNLHLTGSYFIPFDNPAYL
                     KVYINNKLVYSEKLENSSYFKDKLVTIEGSELEQFNNLRIQFSQYPAELLDNYSNLME
                     TYLNPASYFTVAGSLYKNNSFENLLHNFKGEGQILIDENSSDQLLKSTALLYGSLHEK
                     NLGEDNFNVNYLSDHSFARESNNNWYIIMGGDDKNQPINSKLNYQDGRIVIKNEQQTI
                     TEFKTDSNFSYLDLTNYNDKPVMLLDSVKNIESIEDMLNKVNTDGGVTTLRGDLLVYN
                     GENYQTYQLSKEEKPAYFQANYDQLISLFNQYRRYFYLAAIILLVLLLIWIYYRTSHQ
                     KIEGS"
     gene            complement(6739..7683)
                     /locus_tag="VKN64_00925"
     CDS             complement(6739..7683)
                     /locus_tag="VKN64_00925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HMA58822.1"
                     /translation="MKGVISLGEALIDFIPVDKENKLFEKNPGGAPANVAVGLARLGS
                     RTAFIGKVGDDSLGRFLYDTLSKEGINLNNMILTDEAKTALSIVTLDEEGDRSFDFFG
                     EESADRLLTKDEIDEELFGDFKIFHFGSISMINEISRKATLYALELAEQADMYISYDP
                     NLRETLWDDLDKAKKVIKSVLGQVDILKVSKEELDFLTGFAGIEEGAEQIKNKNQLSL
                     VLITDGDKGVYFYKDKLIHVPAQKVKAVDTTGAGDAFVSAFLNRIDESDHKLEEMKNE
                     EIEEICKFANHCSALVVKKEGAMSKLPTKEEVRDFFSE"
     gene            7860..10193
                     /locus_tag="VKN64_00930"
     CDS             7860..10193
                     /locus_tag="VKN64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HMA58823.1"
                     /translation="MSDDFIGKQIKRFDSKDKTTGNQKYLDDYKISGLLHSALLTSPH
                     AHAEIKSIDIEEAKNSKGVKKVVTGKDYPTTLGLYLGDKPPLAVDKVRHYGEPVAAVI
                     AQTKKEAQAAVKKIKVDYEKLAIVDSPKKAKEESSPILHETLADYNHIPDVYPQPGTN
                     IANHTKIRKGNYEEAFTKADYTIEESYSFKPSDHIAMENRSSIAQIKANGDVIIHSTT
                     QAPFVVKELLSFNFEIPAGKINVIAPPIGGGFGGKAGLQLESLAYILSKAVDGRPVKV
                     VNTREDDMVSSPGHIGLEADVKIASDKEGNISGMDLQYYFDAGAYSDYAVNVSRAAAI
                     SCTGPYQVENLRCDSYCMYTNHPFATAFRGFGHIELGYVIERSIDILAKELEMDPVEL
                     RLKNAIQQGDTTPTQSLMGDNTGDLKACIRRTAEMINWEEGSYIKKDDNNVIAKGMGL
                     LWKSPAMPTNTDAGAIITFNEDGSLNLNNAIVEIGQGTKTGLAQIVAEEMQMPIEKVH
                     VKTDVNTNNAPHDWATAASRSLMMAGRAAISACQDAKEQLKETAARVLRCPTEDLTVA
                     NEKVYITDEPHISLDVKDVCLGYVYENGNAVGGQVIGRGNYIARQLSSLDPETGEGRP
                     DLEWTMGAQAVEIELDLTDYSYEITKAASTMDVGKVINPDLARGQAAGAMVMGLSFAR
                     NEGFVFNSREQVQNNVLRDYKIIRYGEHPEFFIDFVETPQGDGPFGARGLGEQGVIGM
                     PGSLANAVSRAVGVEIKELPLLPELIYKYVKEGDSND"
     gene            10186..11022
                     /locus_tag="VKN64_00935"
     CDS             10186..11022
                     /locus_tag="VKN64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HMA58824.1"
                     /translation="MISFDFDYYQADNAEEAVEKYLELKDKNKKVAYYNGGTEVVTYA
                     RKDKMNFDALIDIKNIPACQVLKNNETKIIIGASVTLNEITEKNYFPLLSKSSEVVAD
                     HTTRNHITIGGNLTGRLPFKEAILSLLVAEAKVTTVNKNGFRTRPIIEVFDKRMVLDK
                     EEILLQIIIDKDKVDLPYYQTRKVKQSEIDYPVLHIAALKEDGWLKFALSSLCPFPFR
                     DENIENILNDASIDIESKIEQVIDNLPVPINSNLRGGKEYKEMLFRNSLEECLNSLGG
                     EN"
     gene            11022..11489
                     /locus_tag="VKN64_00940"
     CDS             11022..11489
                     /locus_tag="VKN64_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA58825.1"
                     /translation="MKTYQGHKEITLNVNEEVYNVVVSPNQTLLNVLREKLELTGPKP
                     GCENGDCGACTVLIDDKPYKSCMTLAVEMEGKKITTIEGLKDTAIQEAFIDKEGYQCG
                     YCTSGFIMNAYALLDKYEKPDEDTIKNWLSSNLCRCTGYEGIEKAVKKVIKNR"
     gene            11621..12778
                     /locus_tag="VKN64_00945"
     CDS             11621..12778
                     /locus_tag="VKN64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA58826.1"
                     /translation="MSFKVFNFIYYMTMAAKSFFNIHFSSLGYTSTQIGTINSIARGV
                     ALFILPIWGMASDNFKANKRVLQFAIFGTMAFSLMFLTTANFIIIIILMVFLSSFMNP
                     IRPLYDSLLFSNLGEEGQNKYGRYRLWGSIGYTIIVPILGYFLEGTDVSNTFYVMAGL
                     LFLTLMISFKLPKNKIQEKNDTEKEDTGGRFSNIGELFQNKLFVAFLIYVFFMQITMN
                     GNLTYFPLYVLEYGGKEGLFGLTKMVGAISEILILIFSDKILDRFNIKHIFGVSSSAF
                     VIRWILLGLFPMRSIFLGSQILHSLSFGLFYVTCVNFINKVTKDEVKSTAQNVFTSVY
                     IGLGSIVGNMVGGVIFDKLGGDIMYLTWAAVAFTAGLGYYVFLSQTEKRVA"
     gene            complement(12842..13900)
                     /gene="corA"
                     /locus_tag="VKN64_00950"
     CDS             complement(12842..13900)
                     /gene="corA"
                     /locus_tag="VKN64_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMA58827.1"
                     /translation="MKEFHKKAIDDSGSPPGLLQYDDKVKEEEVTIRVFTYNNEEVIE
                     EDYDEINEEIFEAREGYKKWIDIDGVHNREVISKVGNRFNLHPLVLEDIWNTRQRPKL
                     EEQENALVIILNSIYLNHTEVKTGQISLIINKDVVVSFQEKGIEALDPIRERIRRMKG
                     KIRGSGSDYLAYALIDAIVDKYLAVLEDLGIMIEDLEDKLIEEPEQEDLEMIQELKKE
                     MIFLRKIVWPLIQIDNKMIRSRSDYIKESTEIYLKDVYDHLTQVIDEIEVLRDMLSSM
                     LDIYMSGASNKMNEVMQVLTIVATIFIPLTFIAGVYGMNFKYMPELEMKMGYPIVMVV
                     MLVIAVVMLIYFKWKDWF"
     gene            complement(13915..14712)
                     /locus_tag="VKN64_00955"
     CDS             complement(13915..14712)
                     /locus_tag="VKN64_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HMA58828.1"
                     /translation="MDYVSQIQDLIIQYGLKLIFAIIIIFIGMKVIKYIVNLFEKSFK
                     DKMEPTLMKFLKSVSKALLNILLFLSAAATLGIEMTSFVAVLGAMSFAIGLSLQGSLA
                     NFAGGVLILLSKPFKAGDFIEAVGHRGTVVEIQILYTVINTPDHKQIVIPNGDLSNSD
                     IINYSAYDQRRLDMVFGIGYDDDIKKAKNLLKEIVAENEFILEDPAPVIEVGELAGSS
                     VNFDVKVWIDTANYWKVYYSMQETVKLRFDEEDIGIPYPQMDVHLDK"
     gene            complement(14781..16427)
                     /locus_tag="VKN64_00960"
     CDS             complement(14781..16427)
                     /locus_tag="VKN64_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMA58829.1"
                     /translation="MKRKFLVTTLVLVFVLSMTGVILAQDANFKILFTNDTHGRIEEG
                     DYAGMGFPKVATLVKEHRSEGDVLLLDSGDTFHGQTIVNLNEGEAAVRIMNEMGYDAM
                     TLGNHDFNFGQERIKELEEMANFPLLAANLDPQLVEPYTIKEIAGIKVGIFGLATPET
                     TYKTHPKNVEGLTFRDPSTVAAEMVEELSEQTDMIIALAHLGISEGSEYTSRAVAENV
                     SGIDVIVDGHSHHALEEGMMVNGTLIAQAGEYDKNLGVIDVSVSEGEVTAQASLITKE
                     DAADVEKDQAVLSVIEEVQAANEEITSTVVGKTAVELNGERQYVRTGETNMGNLVADS
                     MLTKTEADVAITNGGGIRASIDEGEVTKGEVITVLPFGNTTVVKKLTGAQLLDTVEHG
                     LSQYPAHEGLFPQVAGMQIIFDGERPAGERVISLKVKGEPVDHDAVYEVATNDFMAAG
                     GDGYEVFQGTETVIEAGGLEELVMNYISQRGTVAPKVEGRIIEVDQQNGNYVYTVEEG
                     DYLAKISRMFNVKVDAIVEANNLENRSMIYVGQELTIPTE"
     gene            complement(16482..16772)
                     /locus_tag="VKN64_00965"
     CDS             complement(16482..16772)
                     /locus_tag="VKN64_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HMA58830.1"
                     /translation="MVKVFWNDKEIARSDETKVVEGNHYFPPESVKMEYFEKSDHHTT
                     CPWKGEASYYHLKDKAQKTNNAAWYYPEPKKEAAKIKDHIAFYTSKVDIQED"
     gene            complement(16885..17418)
                     /locus_tag="VKN64_00970"
     CDS             complement(16885..17418)
                     /locus_tag="VKN64_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58831.1"
                     /translation="MEYKEVIAEVENMFRVIPLQVFRKTEGVVFDLVNMDYLPEVDGI
                     DRVIHAENALSPGTIGEVERPWYMHPHQEDNLLVLYGTRHVDLFTPEHGKIESFEINP
                     HSIKRNGSLIYDGGVILSTSVNVFHRIKTGEEGSASINFAVRHPKFDMKTNFNIYKLD
                     TNTDNYEVIRKGFKDQD"
     gene            complement(17480..17773)
                     /locus_tag="VKN64_00975"
     CDS             complement(17480..17773)
                     /locus_tag="VKN64_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMA58832.1"
                     /translation="MENIIKDMDEIKEKAKILKAISHPIRLCVVKGLMESEGYNVTKM
                     QSCLDIPQSTLSQHLTKLKDLNIIEGERNGVEIKYYVKNDEVKKIINTIFEEK"
     gene            17960..18451
                     /locus_tag="VKN64_00980"
     CDS             17960..18451
                     /locus_tag="VKN64_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HMA58833.1"
                     /translation="MDFKRFFSTLIIYFLFWLAYTASFAAEEMIIGIIIATLLSLFTY
                     KSFTQIKSDNNIFKRIISFVAYIPIFIIEMVKANIDVARRVINPNLPINPGIVKINTD
                     LSSDYAKLFLANSITLTPGTLTMDVKDDSLYIHWIDVASSDKDEQKEQIAGKFEDRLG
                     GIF"
     gene            18451..18702
                     /locus_tag="VKN64_00985"
     CDS             18451..18702
                     /locus_tag="VKN64_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HMA58834.1"
                     /translation="MLNLVFILIFLSIVFGAYRFIKGPSSSDRVVALDTLTTIVSVAL
                     VVGSLFFKNYILLDISFVFAALSFTSVIVIARYLEGGKS"
     gene            18699..19049
                     /gene="mnhG"
                     /locus_tag="VKN64_00990"
     CDS             18699..19049
                     /gene="mnhG"
                     /locus_tag="VKN64_00990"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HMA58835.1"
                     /translation="MILLSFIGYVLIILGSIFLLLGSLGIVRMPDLYNRIQAGTKATT
                     LGALSAIIGVGLVEPSWFFKTLIIAVFIVLTNPIGSHSIARSSYKRGLKPVITSEIDE
                     YDEENLTDERSDSA"
     gene            19046..19288
                     /locus_tag="VKN64_00995"
     CDS             19046..19288
                     /locus_tag="VKN64_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase subunit MbhD domain-containing
                     protein"
                     /protein_id="HMA58836.1"
                     /translation="MMIISYLLILLMIIGALAAVLFENLLNSIISLGLISLIAAALMY
                     FLKAPDVAMTEAIIGAGLTTSIFIFTLLKIRRSDNQ"
     gene            19285..20037
                     /locus_tag="VKN64_01000"
     CDS             19285..20037
                     /locus_tag="VKN64_01000"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HMA58837.1"
                     /translation="MRFRDYLSLFMVLILGFILFQAFAGGSENTLSIPQFGEANLENR
                     VSEHYINKSVNSETESITFNQTLNPESGSANYVTSIIANYRALDTLGEVTVLFIAAAG
                     LAFVLGGNQKNYAFAEDASFIVRTGVKILFPLMFLLGIYIIVHGHLTPGGGFQGGAVI
                     ATAILLKFLAYKDYKLNHTGLSLLESFAGISFLIIGLIGLLTNGSFLTNFLGNGTLGT
                     LFSAGIIPIIYILIGIKVGAELSGIISNLINA"
     gene            20066..20428
                     /locus_tag="VKN64_01005"
     CDS             20066..20428
                     /locus_tag="VKN64_01005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HMA58838.1"
                     /translation="MAFLNQWAYIGGSIILILIGLFGVLTRKNLIKIVVSIGIMDTGV
                     NLLLVSLGYINNGIAPILTEANASNSLNFVDPLPQALVLTAIVIGVAVLALALSIVIK
                     IYENQNTLKIDEIRGLKL"
     gene            20425..>20885
                     /locus_tag="VKN64_01010"
     CDS             20425..>20885
                     /locus_tag="VKN64_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58839.1"
                     /translation="MNASLLIAVPLLTAFVIPLIGKLHKKIPSFLVLLSTLFNIIITF
                     SLYGAVMESPIVIQIGNWTVPFGINLYVDKLALVSLMIISTITFLISIYNIRNTDELN
                     ETKFNTMFLLAFTGINGIVLTGDIFNLFVFIEIAAISTYVLATMKRKESYQA"
BASE COUNT         6668 a         3685 c         3375 g         7157 t
ORIGIN      
        1 attgaccggt agttttaaat acctaccctg gtctggtcca tattccatat tgtaaagtcc
       61 agtcgtatta tcttcattta ttataccatt tactatttca ataattagac tattatattt
      121 aaatagatct gaggttaaaa aatgatcagg ggccagttcg gttttatcgg tataaatact
      181 ggttacataa gttggccggt tggcatcttc taccgccctg aacataccat aattaccaag
      241 attaactcca ctcattatga tatcaacttt tctatttatt agggtttcgg ttgcctgttt
      301 tgtctttaat gggttattaa aactaccaac atagatatat tcaaattcta cctgaggatt
      361 gtgctcctga acagcctgct ggatcgcatt tacaacccca atggtccagg agatcttaag
      421 tccgcctaca aaacctattt tgtcagtttc ggtaacttca cttgccaaag cacctaaaac
      481 atagtggttt atataataat ttcttttaat ctgaatgaca ttatcaaaat cctctgataa
      541 aggtaacttc tggatagtta tttgccaaat caaagatacc actgttaaat tgagtcccgt
      601 gtatccagat tatattatat cataaagaaa tatattctct tactacccgc ggtgcatcgg
      661 gtaccgctac ctgttctaaa taagaaactt ctaaacccag ttcatcgttt acattttgta
      721 aggccttata gccaatggta ttccagtccg catcctggat agatcccgga aagacagctg
      781 ccatcttata ttcagcttct tgagcctctg ttatatttcc aagtggaaat aaagttaaaa
      841 ctaacaacaa tattactgcc gtaacaagtt tattattgat atttatattc actatttttt
      901 tctcctcatt taattcctag tatatttgtc agatttaatt atatagtaat ataataatgg
      961 gataaatgca atattttcct atttatctcg ttaatatatt aataaaagga taagaaaagg
     1021 ttctctagaa taaagtattg agaagtatta tttttaggtg gtgtatataa aatgaaaagt
     1081 aaagataaaa agggagttaa gatctgtaaa cattatgatc tctgcggcgg ctgcagcagt
     1141 ttagatgttc cctatcaaaa acagctcgaa cagaaaaaag caaaggtaaa aaattatttt
     1201 ttagaaaatg atataatgga atttccgctt agcgaagtta ttgaaagtcc taagatctat
     1261 gaatatagaa accatatgga gcttagcttt ggtgatttag agatcggggg taaactgcag
     1321 ttaggactcc atccccgtgg taaaagatat gaggtagtaa ctatagacgg ctgttattta
     1381 atagatgaag attttagaaa gattattaaa actatgatcg actacttccg tcaaacaaac
     1441 cttaaaaaat accatgtaaa attaagagaa ggctatttaa gaaatgtaaa actaaggaag
     1501 ggaattaata ccggtgagat cttagtaaat ttaattacta attctcagcg ggacccagat
     1561 ctaagcgatc tatttgaaga gttaaaggac ttggattata aagatgaact ggtaggaatt
     1621 ttacatacca ttaatgatga ttatgccgat gcagtcaagg cagacgaagt tagaacttat
     1681 tatggccggg attattttta cgagaaatta ctcggttata aattcaaagt aagccctctt
     1741 gattttttcc aggtcaatac aaaggcggcc gaaaagcttt ataaaattgt tatagagtat
     1801 tttaaaggta gtaaggaaca aaaagtatat gatctttatt caggtactgg ctcaatttcc
     1861 caggtaatat caccccatat caaaaaagta atcggtatag aacttgatga aagtgccgtt
     1921 aaaaaggcca gggctaactt aaaattaaat aatgttaata actgtgagtt cattcagggg
     1981 gatgtaaggg aagtattgaa aaataaaaaa gaaccggtta acaaggtggt cgtcgatcca
     2041 cctcgccccg gtttacattc agaagttaca agaagtatta ttgagatttc tccacaagaa
     2101 attatctatg tatcatgtaa tccacaaaca caggcccaag atcttaaaaa attattatac
     2161 aatgattacc atattgaaga cataaaatta gttgacatgt ttccccatac cactcatatt
     2221 gagagtgtag cccatattgt taaagattaa atattagtta gtttccttta ttttttctag
     2281 aaagtttatc tgcataaaca tgatctggtc ttcctgcata tcatgtgccc tatttaatct
     2341 gtaatgaggt ctcttaatta ataacataat tgatttaatg aaagcatatg actttattac
     2401 ccaatgtacg ggtaataaag gggtaatcaa aatcctatca agatatttaa tctttaaatc
     2461 attactgaaa atataataag tgttaaatat taaaaagaga accagattaa aaactgtgaa
     2521 tagaaactgc ggctgccaag ttaaaaaagt cataatacga ccggctgcta aaaaaactgc
     2581 tgatattaat attaaacatg tatataaaaa gggtagtaaa agttccccac ctatcgtcag
     2641 gttgttaaat aatatgtcct ttaacccatc ttcttttaac ttggagatgg gttttctgtt
     2701 atagacaagc atggtctgca tataaccctt aagtgagtta gtaaagttaa aaaaccagtt
     2761 gttgaagtta tcactgctta actgaaatgt tttcgaattt atgatgccta tttcataacc
     2821 tcgggcagcc gctttcctga ttatttctaa attctcggct gtgttatata aatcccagcc
     2881 tccaaattgt ctaatagcag ccgttttata atggtaaatc ccttcatcta aataggagaa
     2941 aaatttgtat ttcaaattat tttcaatact taaatattta ctaaataaac tattattact
     3001 accggctgct tctaagggag actggaggca gaaataattt ctttgagagt tttcaaactt
     3061 aaagactgac tttttaagct ggtcagcctc tggaatagtg cttcccttat aaatagttat
     3121 atactttcca cttgctaaat ttaagccgaa attataaatc tctatttctg ttcttaattt
     3181 actggcaggt atagtatata ttaaccagct ttcattattc aatgccagat tttttatctt
     3241 gtcttttagc tttttatcaa ctaaaaatat tacttccatc ttttctgaag ggtaatccat
     3301 tttctttaaa cttaaaatca aattgtttag atcacttgtg gattgggtaa ccggaattaa
     3361 gacagtataa acaggtaaac tatcctggtc cctgatattt ttttctttac tcgctccttc
     3421 tttatctatc ttgttttttt tagctagata tattataaag cggctgatta aaaataataa
     3481 gaataatgta ttaagagtat aaattaagat atctaccgtg ctattaaaat aaaagaataa
     3541 tccagttata agtaaaatcc caaagatggc tactcctaag agctgtcctc tcgtaaagac
     3601 atttaaagct gtttcattat tttttctttt aatattattt ttttgcttct ttattatttc
     3661 atctttaaaa gcatattcaa tgaaatattt gatttctttt gggtaaagtt ctactttctc
     3721 aatcaagata tctccaaatt tgttttttaa aaatctatcg atactcttat ctttaaaatc
     3781 aaaaactata attttgatga tattattttt aaaataaagc ggcatgaaca gtttttcatt
     3841 taaaaaagtg gggttaaatt ttttaacaaa accataatca atttcttctt taagggggat
     3901 cgttttttca gataattgat tagatatata gttctcggat aaatcggcca ggatcttata
     3961 tatctcaagc ccctctacca tcttgtttct aatcaaaatt ttagataacc gctcattggt
     4021 atcggtatgt atgtcatagg cattatttaa ttgtcttttg gttattaggt tttttctgag
     4081 taaagtttta cccaataagg tttgctcaat caaccataca cacctacctt tatgatcctt
     4141 caattttttg atgcgaagtc ctgtaataga tccaaattaa taacaagacc aataatataa
     4201 tggcagccag gtaaaaatat cgcctatatt gattaaataa agatattaac tggtcataat
     4261 ttgcttggaa gtaagcaggt ttttcttcct tagacagttg gtatgtttga taattttcac
     4321 cattatagac taagaggtca cctctaagag tggttactcc tccgtctgta ttaactttgt
     4381 ttaacatgtc ttctatactc tcaatatttt ttacgctatc caataacata actggtttat
     4441 cattatagtt ggtaaggtct aaataactga aattactgtc cgttttaaat tcagtaattg
     4501 tttgctgctc gttttttatt acaatcctgc cgtcctgata attgagcttt gagttgattg
     4561 gctgattttt atcatccccc cccattatga tataccagtt attattactt tctctagcaa
     4621 aactatggtc tgataggtag ttgacattaa agttatcttc tcctagattt ttctcatgca
     4681 gtgaaccgta taagagggca gtagacttta ataattggtc ggaactattc tcatcaatta
     4741 atatctgtcc ctctccttta aagttgtgca gtaaattttc aaaactatta tttttatata
     4801 agctgccagc cactgtaaaa taactggccg ggttaagata ggtttccatt agattactat
     4861 aattatccag cagttcggcc ggatactggc tgaactgtat cctcaagtta ttgaactgtt
     4921 ctaactcact gccttcaatt gttactaatt tatccttaaa atagctgctg ttttctaatt
     4981 tttcactata aactaactta ttatttatat acacttttaa ataagcgggg ttatcaaagg
     5041 gaataaagta actaccggtt agatgcaagt tgaaatctct cggccagtta gtaaaaatat
     5101 taatcggtaa atggtaatga tagttgatct ctccccaacc ctttctatta gcttctagaa
     5161 agtcggtctg ataagaactt aacttatagt taacatcctg cttaagatta tattcagtaa
     5221 catctaagaa atttgcatta actaaattaa ggaaatcgga aattaaaaaa tctatatctt
     5281 cagaagataa atgcagagtc ataaattcat ctaaacttgt cttatcatac tcttcagata
     5341 ttacaatatt tcttattcta ttatctaaat tgttaaattc tacatcttct gaacttaatt
     5401 tcaactctat ctcgggcggt aagtctttta ataacctatt taggtaggta tataacttga
     5461 tataatcatg aaattcctgc ctacttaatt taccttcata gtataaatta aacttttcgg
     5521 cttctattcc caagaaattg ttgatttgat agttctgatt atcttttaat ttaaagccga
     5581 taccactatc attgtcgatt ttaatccaca tattttcata taatgatttt gtataatctt
     5641 tctgtttatt tctaagttgg gctttaatct ctatctgaag cttatttata ttttttaatt
     5701 tcagggggaa tattaaactg tattggtttt gacctgactt gaaattgtac tttttgacag
     5761 gttggttgtt tatcataata tcaatatttg ataattcccc aataaattgt gaagttatta
     5821 aatttaattt taaatatatt tggtcctcaa taaccttatt tgataatgga atattaatga
     5881 taaaagaatt ttgccagccc tttaagatga tatcattttt ataaccaaaa tcttttaatg
     5941 tataaataat ttcattatta tttttttgtt gaaatatatc aattaattca ttatcccgta
     6001 aaactaaatg ataattatta tttaatttta ttttatcatt ataagaatca gtggagtatt
     6061 taacctcttc accgttgggg aataagaact gggaattgtt ttcagttttt tgaactaaaa
     6121 aggcggtttt tatctcaaac tgattatttt ctttataata atctaagtct tttttaacag
     6181 aaggcttttc aacctgatct gtgtcttctt ctgtgctgac ctcttcacta ttacttattt
     6241 cctttttgtt atcttctact tgatctttat tctttttatt ttccttatta atatcatcct
     6301 tatcctcagc agctatttca tcttctttta cttccttact tttttgagct gtttgctctt
     6361 tagtctctga ttctggttgt tttttgttat cttcggaagg agttctactt ttagtaaatt
     6421 caaaatccac ttttctaata taggcttctg gtactatatt tttaacctca gctaaagtat
     6481 ctgcagcgga attatagtta ttaaattgac ctacaaatac ataatatcta ttattttgtt
     6541 gatatttata agtttcaaat ccctgctcgc ttaatttttg agatagattg agtgcattat
     6601 tttctttact aaaataaccg gtttggattg tataagaagc tgcaccaaca gctgttgttg
     6661 ttagtaatag gataaagata gttattaaaa aagaattaaa tttaatctcg atcacctctt
     6721 tgggtttact ctccccaatt attcactgaa aaaatctctt acctcttctt ttgtcggcag
     6781 tttggacatg gcaccttctt ttttaacaac cagggccgaa caatggttgg caaacttaca
     6841 gatttcttct atttcttcat ttttcatctc ttctaacttg tgatcactct catcaatacg
     6901 atttaaaaaa gcagatacaa aggcatctcc ggctccggtg gtatcaactg ctttcacttt
     6961 ctgagccggt acatgaatta atttatcctt gtaaaagtag acgcccttat ctccatctgt
     7021 gattaaaaca agactcaatt gattcttatt tttaatctgt tccgcccctt cttcaattcc
     7081 ggcaaaacct gttaaaaaat ctagttcttc tttcgatact ttcaagatat caacctgtcc
     7141 caacaccgat ttaattacct ttttagcctt atctaaatca tcccagaggg tttctcttaa
     7201 attgggatca tatgatatat acatatcagc ctgctcggct aactctaatg catataaggt
     7261 ggccttacgt gaaatttcgt taatcataga aatagaacca aaatggaata tcttaaagtc
     7321 accaaataat tcttcatcaa tttcatcttt agtaagtaat ctatcagcac tttcttcacc
     7381 aaaaaagtca aaactacggt ccccctcttc atcgagtgta actatactca gggcagtttt
     7441 ggcctcatcg gtaagaatca tgttattcaa attaatcccc tctttagaaa gggtatcata
     7501 aaggaatcta cctaaagaat catcacctac tttaccaata aaagcagttc tacttcctaa
     7561 tctggccaga ccaaccgcta cattagcggg ggccccaccc ggattttttt caaataattt
     7621 gttttcctta tcaactggta taaagtctat taatgcttca ccaagagata ttactccttt
     7681 catttatgaa aacctcctaa gaagaatatt tttatcatta ttattaatat tatacgaaaa
     7741 gacataaaag tcctacatta aactcatatt atttttttac aaataaaaag gatttggagt
     7801 aagtccattt aatatataaa aataataatc atttctgata agagagaggt gagttgattt
     7861 tgagtgatga ttttatcgga aaacagataa aaagatttga tagtaaagat aaaaccacgg
     7921 gaaatcaaaa gtatttagat gattacaaga ttagtggttt actgcattca gcccttctaa
     7981 caagcccaca tgctcatgca gagattaaaa gtattgatat tgaagaagct aaaaattcaa
     8041 aaggagtcaa aaaagttgtt actgggaagg attatcccac aactttaggc ctatatttag
     8101 gagataaacc accccttgca gttgataagg tccgacatta tggagaaccg gtcgcggccg
     8161 ttattgcgca aactaaaaaa gaagcccaag cggcagttaa aaaaattaag gtggattatg
     8221 aaaaattagc aattgtagat tctcctaaaa aagcaaagga agaatcttca cctattttac
     8281 atgaaacctt agcagactat aatcacattc ctgatgtcta tccccaaccc ggtaccaata
     8341 ttgcaaacca taccaagata cgcaaaggta attatgagga agcctttaca aaggctgact
     8401 atacaataga agaatcatat tctttcaaac caagtgacca tatagctatg gaaaataggt
     8461 cctctatcgc ccagataaag gcaaatggag acgtaattat tcattctact acccaggcac
     8521 cttttgtagt aaaagaacta ctttccttta acttcgaaat accggccggc aagataaatg
     8581 taattgctcc ccctattgga ggcggtttcg gaggaaaagc cggactacag ctggaaagtt
     8641 tagcctatat tctctccaag gcggtagatg gtcgtcccgt caaagttgtc aatacccgtg
     8701 aagatgacat ggtctcttca cccggccata tcggtttaga agcagatgtc aaaattgcca
     8761 gtgacaaaga aggtaatatt tccggtatgg acttacagta ttattttgat gccggtgcct
     8821 attcagatta cgctgtcaac gttagtcggg cagccgccat ttcctgtact ggaccttatc
     8881 aggtagaaaa tttaaggtgt gattcctact gtatgtatac aaatcacccc ttcgctaccg
     8941 cttttcgggg ctttggacat attgaattgg gatatgtaat tgaaagaagt attgatattc
     9001 tggcgaaaga attagagatg gaccctgtcg aattgaggtt aaaaaatgct atccagcagg
     9061 gtgatacaac tcccactcaa agtctaatgg gagataatac aggggacctc aaagcttgta
     9121 ttagaagaac agctgaaatg ataaactggg aagaaggatc atatattaaa aaggatgata
     9181 ataatgtgat agcaaaaggg atgggacttt tatggaaatc acccgcaatg cccactaata
     9241 ccgatgccgg tgctattatc acctttaatg aagacggtag tctgaactta aataatgcga
     9301 tagtcgaaat cggccagggg accaaaacag gtctggccca aattgtggca gaagaaatgc
     9361 agatgccgat agaaaaagtc catgttaaaa ccgatgtaaa tacaaataat gctccccatg
     9421 actgggcaac agcagccagc cgaagtttaa tgatggccgg tcgagctgcc atcagtgcct
     9481 gtcaagatgc taaagaacag ctaaaagaaa cagctgcccg ggttttaagg tgtccgaccg
     9541 aagacttaac tgttgccaat gaaaaggtat atattaccga tgaaccccat atttcactgg
     9601 atgtaaaaga tgtatgtcta ggttatgtct atgaaaatgg taatgctgtc ggtggccagg
     9661 tcatcggccg cggcaattac attgcccgtc aattatcaag tcttgatccc gaaactggag
     9721 aaggtagacc tgatctagaa tggacaatgg gagctcaggc ggttgaaata gaactagact
     9781 taactgatta ttcctatgaa ataaccaaag cagcctctac catggatgta ggtaaggtaa
     9841 taaatcctga tcttgccaga ggccaggccg cgggagctat ggttatggga ctaagttttg
     9901 cccgtaatga aggctttgtc tttaacagcc gtgaacaggt ccagaataat gtattaagag
     9961 attataagat aatcagatat ggtgaacatc ctgaattctt tatcgacttt gttgaaactc
    10021 cccagggtga tggaccattt ggagcaaggg gcttaggtga acagggagtt attggtatgc
    10081 ccggttcact ggccaatgca gtttccagag cagttggcgt agaaataaag gaactccccc
    10141 tgctaccaga gctaatctat aaatatgtaa aggaaggtga tagtaatgat tagtttcgat
    10201 tttgactatt accaggcaga taatgctgaa gaagcagttg aaaaatatct ggaattaaaa
    10261 gataaaaata aaaaagtagc ttactataat gggggaaccg aagtagttac ctatgctaga
    10321 aaagacaaaa tgaactttga tgctttaata gatattaaaa atataccagc ctgtcaggtt
    10381 ttaaaaaata atgaaactaa aataataata ggggcctcgg ttactttaaa tgaaataaca
    10441 gaaaaaaatt attttccgct gttaagtaaa tcatctgaag tagtagccga ccatacaacc
    10501 agaaatcata ttacaatcgg gggtaactta acagggaggc tgccttttaa ggaagctatc
    10561 ttatccttat tagtagctga agcaaaagta accactgtaa ataaaaatgg ttttaggacc
    10621 cggcctataa tagaggtttt tgataaaaga atggttttag ataaagaaga aatcctactt
    10681 caaataataa tagataaaga taaagttgat ttgccctatt atcaaaccag gaaggtaaaa
    10741 caaagtgaaa tagactatcc agttctacac attgcggcct taaaggaaga cggctggcta
    10801 aaatttgcac tttcttcctt gtgtccattt cctttcaggg acgaaaatat tgaaaatata
    10861 ctaaacgatg cttctataga tatagaaagt aaaattgaac aggtaataga taacctacct
    10921 gtacctataa attcaaatct aagaggaggt aaagagtata aagaaatgct ctttagaaat
    10981 tctttagaag aatgtttaaa ctccctcgga ggtgaaaatt aatgaaaact tatcaaggac
    11041 ataaagagat cactttaaat gtgaatgagg aagtatacaa tgttgtggta agtcccaatc
    11101 aaacattatt aaatgtccta agagaaaaat tagaattaac tggacctaaa cccggttgtg
    11161 aaaatggtga ctgtggggcc tgtaccgtat taatagatga taaaccttat aaatcatgca
    11221 tgacattagc tgtagaaatg gaaggaaaaa agataactac gattgaaggc ctaaaagata
    11281 cagccatcca agaagccttt atagataaag aaggttatca gtgtggttac tgtacctctg
    11341 gcttcattat gaatgcctat gccctcttag ataaatatga aaaacctgac gaagatacta
    11401 tcaaaaattg gttgagcagt aatctctgcc gatgtacagg ctatgaagga attgaaaaag
    11461 ccgttaaaaa agtaataaaa aacagatgaa acttgatata ttaacttaat attatctatc
    11521 agccccaaat ttaattgggg ctttttttta ttgaattata gaaagatttg tgatattgta
    11581 cttatgttaa aataaagata ccggaaagga ggcacataac ttgtcattta aagtatttaa
    11641 ttttatctat tatatgacca tggccgcaaa gtcatttttt aatatacact ttagcagtct
    11701 cggctatact tctacccaga ttggtacgat taactcgatt gcaagagggg ttgccctctt
    11761 tattttacca atttggggta tggcttcaga taactttaaa gccaataaaa gagttctgca
    11821 atttgcaatt ttcggtacga tggctttcag tttaatgttt ttaaccacag ctaatttcat
    11881 cattattatc atcctaatgg tctttttatc ttcatttatg aatccaatta gacccttata
    11941 tgatagtttg ttgttcagta atttaggtga agaaggacaa aataaatatg gtcgctatag
    12001 attatggggt tcaatcggat atacaataat tgtccctatt ttgggttact ttttagaagg
    12061 aacagatgta tctaatacat tttatgtaat ggccggtcta ttatttttaa ccttaatgat
    12121 cagttttaaa ctgcccaaaa ataaaatcca ggaaaaaaat gatactgaga aagaagatac
    12181 aggcggaaga ttctccaaca taggcgagct atttcaaaac aaattatttg tagcattttt
    12241 gatctatgta ttcttcatgc agataacaat gaatggtaac ttaacctact tcccacttta
    12301 tgtgcttgaa tacggtggta aagaaggact atttggtcta actaagatgg tgggagcaat
    12361 cagtgagata ttaatattga tcttttccga taaaatacta gatagattca acattaagca
    12421 tatctttggc gtttcctcat ctgcttttgt tattagatgg atcttattag gtctattccc
    12481 gatgcgtagt attttcttgg gttctcaaat tttacacagt ctctctttcg gcctgtttta
    12541 tgtaacctgt gtaaacttta ttaataaggt tactaaagat gaagtaaaat cgactgctca
    12601 aaatgtcttt acttctgtct acatcggatt aggaagtatt gtaggaaata tggtcggagg
    12661 agtcatcttt gataaactag gtggagacat catgtaccta acctgggctg ctgttgcctt
    12721 tacagccggt ttaggatatt atgttttctt atcacaaact gaaaaaagag tagcttaatc
    12781 aaaaaactac tcttttttca ctctttttta tttactttta gaataatttt gttaagtata
    12841 atcagaacca atccttccat ttaaagtaaa taagcataac tacagcaata accaacatta
    12901 caaccataac tatcggatat cccatcttca tttcaagctc aggcatgtac ttaaaattca
    12961 tcccatatac accggcgata aaggtgagtg gaataaagat agtagctaca attgttagga
    13021 cctgcattac ttcattcatt ttattgctgg cccctgacat ataaatatca agcattgagc
    13081 ttaacatatc tctcagcact tcaatctcat caattacctg agttaaatgg tcatagacat
    13141 ccttgagata aatttcggta gattctttaa tataatcact tctagatctg atcatcttat
    13201 tatcaatctg tattaaaggc cagacaatct ttcttaaaaa gatcatttct ttttttaatt
    13261 cctgaatcat ttctaagtct tcctgctctg gttcctcaat taacttatct tctaaatcct
    13321 cgatcataat acccaaatct tctaaaacag ccagatattt atcaacaatg gcatcaatta
    13381 gggcataagc taagtaatca ctgcctgagc ccctgatctt acctttcatt cgtctgattc
    13441 tctcacggat gggatctaaa gcctctatcc ccttttcctg gaaagatacc accacatctt
    13501 tattaataat caaactaatc tgaccggttt taacttcggt atgattaaga tagatagaat
    13561 ttaaaatgat gaccaaagca ttttcttgtt cttctaattt cggcctctgt ctggtattcc
    13621 aaatatcttc aagtacaagc gggtgtaagt taaacctatt acccacttta gaaatcactt
    13681 ctctattgtg aacaccatca atatcgatcc acttcttata tccttccctg gcttcaaata
    13741 tttcttcatt aatttcatca taatcttctt ctataacttc ttcgttattg taagtaaaga
    13801 ctctaattgt aacttcttct tcttttactt tgtcatcata ctgtaatagt cccggcggac
    13861 taccactatc atcgattgcc tttttgtgaa actctttcaa tgtatatacc tccgttattt
    13921 atcaagatgt acatccattt gtggataggg aatacctata tcttcttcat caaatcttaa
    13981 tttaacagtt tcctgcatac tgtaataaac tttccaatag tttgcagtat caatccatac
    14041 ttttacatca aaattaactg aactaccagc taattcacca acttcaatta ctggagctgg
    14101 gtcctctaaa ataaattcat tttcagctac aatttctttt aataaatttt ttgctttctt
    14161 gatatcatca tcataaccga taccaaatac catatcaagc cttctctggt cataagcaga
    14221 ataatttata atatcagaat tggaaagatc accatttggt atcacaatct gtttatgatc
    14281 aggtgtattg attactgtat ataaaatttg tatttctaca acagtaccac gatggccgac
    14341 tgcttcaata aagtctcccg ctttaaatgg cttagagaga agaattaaaa ctccaccagc
    14401 aaagtttgct aaacttccct gtaaggaaag gcctattgca aatgacatag cacctagaac
    14461 agcaacaaaa gaagtcattt caatacctaa tgtagcagca gctgatagga ataataagat
    14521 gtttagtaag gctttagaga cagattttaa gaacttcatt aaagttggtt ccatcttatc
    14581 tttaaaggat ttttcaaata aatttactat atacttaatt actttcatac ctatgaagat
    14641 tattattatt gcaaagataa gttttaaacc atattgaatt ataaggtctt gaatttgact
    14701 aacgtaatcc ataaaaagac acctctttct tataaaaaaa tgaaggggta gcagagctac
    14761 ccctttagcc tgctatttaa ttattctgta ggaattgtaa gttcttggcc aacataaatc
    14821 atgctgcggt tttcaagatt atttgcttct acaatggcat caacttttac attgaacatt
    14881 ctagaaattt tcgcaagata atctccttct tctacagtat atacatagtt accattttgt
    14941 tggtcaactt caattatcct accttccact tttggtgcaa ctgtacctct ttgactgata
    15001 tagttcatta ctaattcttc taaaccaccg gcttcaataa cggtttcagt tccttggaat
    15061 acttcataac cgtctccgcc agcagccata aagtcattag ttgctacttc atatacagca
    15121 tcatgatcaa caggttctcc ctttactttt agactgatta ctctttctcc agctggtctt
    15181 tcgccatcaa agataatctg cataccagct acctgtggga atagtccctc atgggcagga
    15241 tactgtgata agccgtgttc aacagtatct aagagttggg cacctgttag ttttttaact
    15301 acggtagtat taccaaatgg aagaacggtg attacttcac ctttggttac ttctccttcg
    15361 tcgatagaag ccctaattcc accaccattg gtaattgcta catcagcctc ggttttagtt
    15421 aacatggaat ccgcaactaa gttacccatg ttagtctcac cagttctcac atattgtctt
    15481 tccccattta actcaacagc agtcttacca acaacagtag atgtgatctc ttcattggca
    15541 gcctgaactt cttcaattac acttaaaact gcctgatctt tctctacatc agctgcgtct
    15601 tcttttgtaa ttaggctagc ttgggctgtg acttcacctt cagagaccga aacatctatc
    15661 acacccagat ttttatcata ttcaccggct tgtgcaatta aagtaccatt aaccatcata
    15721 ccttcttcta atgcatgatg actgtgtccg tcaacgatta catcaatacc ggatacattt
    15781 tcagctacag ctctacttgt gtattcacta ccttctgata ttccgaggtg agctagagca
    15841 attatcatat cggtttgttc agatagttcc tcaaccatct cagctgccac agtagaagga
    15901 tctctaaatg tcaaaccttc tacattttta ggatgagttt tataggttgt ttcaggagta
    15961 gctaatccaa agatacctac cttaataccg gcaatttctt tgatggtata aggttcaact
    16021 aactgcgggt ctaaattggc agccagtaac ggaaaattag ccatctcttc taattcttta
    16081 attctttcct gaccaaagtt aaagtcatgg ttacctaaag tcattgcatc ataacccatc
    16141 tcattcatta ttcttacagc tgcttcacct tcattgagat ttacaattgt ctgtccatga
    16201 aaagtatcac ctgaatctaa caataaaaca tcaccttcac tacggtgctc tttaactaaa
    16261 gtagccacct taggaaagcc catacctgca tagtcccctt cttcaattct accatgggtg
    16321 tcattggtaa atagaatctt aaagttagca tcctgagcta aaatgacacc tgtcatagat
    16381 aaaacaaata ctaaaaccaa agttgtaact agaaactttc ttttcattaa catattcccc
    16441 cctaaattag ttttatttat atgattttat tgttaccttt attaatcttc ctgtatatca
    16501 accttactag tataaaaggc tatatgatct ttaattttag cagcctcctt tttcggttct
    16561 ggatagtacc aggccgcatt atttgtcttt tgtgctttat ctttgaggtg ataatagctg
    16621 gcctcgcctt tccagggaca ggttgtgtgg tgatcgcttt tttcaaaata ctccattttg
    16681 acactttcag gaggaaaata gtggttgccc tcaacaactt tagtttcatc tgacctggct
    16741 atttccttat cattccagaa aactttaacc atcagtctcc cctcctttta attctattgt
    16801 attatatata attcaatgat ttttcctaat tacctgcatc taatcaatta ttaattaagt
    16861 tatattttca aaaaatttaa gttgttaatc ttggtcttta aaaccctttc taataacttc
    16921 ataattatca gtattcgtat ccaacttata gatgttgaaa ttggtcttca tgtcgaattt
    16981 gggatgtctt actgcaaaat ttattgaggc cgaaccttcc tcaccggttt taattcggtg
    17041 aaaaacatta actgaagttg ataaaatgac tcccccatca taaattaagc tgccgtttct
    17101 ttttatagaa tgaggattaa tctcaaagct ttcaattttt ccatgctccg gggtaaacaa
    17161 atcaacatgt ctggtcccat acaaaaccaa aagattatct tcctggtgag gatgcatata
    17221 ccagggtctt tcaacttctc cgatagtgcc cggggacaaa gcattttcag catgaataac
    17281 acggtcaatt ccatcaactt ctggcagata atccatatta accagatcaa atacaacacc
    17341 ctccgtcttt ctaaaaacct gcaagggaat tactctaaac atattctcta cttcagcaat
    17401 cacttcttta tattccattc tcttatcctc cttttttaaa attaaaaaat cctccccaat
    17461 taaaggaagg ataattgctt tatttttctt caaatatcgt atttattatt tttttaactt
    17521 catcattttt gacatagtac ttgatttcaa cgccatttct ttcgccttca attatattta
    17581 ggtctttaag ctttgttaga tgttgtgaaa gtgtggactg aggtatatct aaacaggact
    17641 gcatttttgt cacattatat ccttcactct ccataagtcc tttaacaaca cataaacgga
    17701 ttggatgtga gatagcttta aggattttcg ctttttcttt aatttcatcc atatctttaa
    17761 ttatattttc caaagttggg tcccccttaa tcgaattcta tatctatata ttatgaaata
    17821 atgatattta agtcaagaaa tttgtgaaaa attataaatt acatttgaca aatatattta
    17881 tatatcataa tatttagata tagtgataat tgtttagact tccaagtgaa ctatatcaca
    17941 cttcaaaggg ggattagaat tggattttaa aaggtttttt tctacactta ttatttattt
    18001 tctcttctgg ttagcctaca cagccagctt tgccgctgaa gagatgataa taggtattat
    18061 aattgcgact ttgttgtcac tttttacata taaaagtttc acacaaataa aaagtgataa
    18121 taatattttt aaaagaatta tatcttttgt agcttatata cctattttta ttatcgagat
    18181 ggttaaagct aatattgatg tagctcgccg agtaataaat cccaatttac caatcaatcc
    18241 aggcattgta aagatcaata cagatttaag ttctgattat gctaaattat ttttagctaa
    18301 ttcaatcacc ttaacacctg gtactctaac catggatgtt aaagatgata gtctatatat
    18361 tcactggatt gatgtggcaa gttctgataa agatgaacaa aaagaacaaa ttgccggtaa
    18421 atttgaggat agacttgggg ggatttttta atgcttaatc ttgtgtttat tttaattttc
    18481 ttatcaatag tttttggagc ttacagattt ataaaggggc cttcatcttc tgatcgggtt
    18541 gttgcccttg atactttaac tactattgtc tcagttgctt tagttgtagg ctccctgttt
    18601 tttaaaaatt atattttact cgatatctca tttgtttttg ctgccctttc ctttacaagt
    18661 gttatcgtaa ttgcccgtta tttagaaggt ggtaaatcat gattctttta tcatttatcg
    18721 gatatgtatt aattatttta ggcagtatct ttttgctttt aggatcactg ggtatagtaa
    18781 gaatgccgga cttatataat agaattcagg ctggtaccaa ggcaactact ctcggtgctt
    18841 tatcagcaat aatcggagta ggattggtag aaccaagctg gttttttaaa accttgatca
    18901 tcgctgtctt tatagtttta acaaacccaa tcggcagcca tagtattgct cgatcatctt
    18961 ataaaagagg tctaaaaccg gtaattacct cagagatcga tgaatatgat gaagaaaact
    19021 taactgatga aagaagtgat agtgcatgat gattattagt tatttactta tcttgttaat
    19081 gattattggg gctttagcag ctgttttatt tgaaaattta ttaaattcaa taatttcatt
    19141 agggttaata agtttaatag ctgccgcctt aatgtacttt ttaaaagctc ctgatgtggc
    19201 aatgacggaa gctattattg gggctggcct gaccacttcg atctttatct ttaccttatt
    19261 aaaaataaga aggagtgata accagtgaga tttagagact atctctccct ctttatggtt
    19321 ttaatattgg gttttattct ttttcaggct tttgcaggtg gtagtgaaaa cactcttagt
    19381 attccccagt ttggcgaagc aaatctagaa aacagggttt cagaacatta tattaacaaa
    19441 agtgtcaatt cggaaactga aagcataaca ttcaatcaga ctctgaatcc agaaagtggt
    19501 tctgctaact atgttacctc tattattgct aattataggg ccttagatac tttaggggaa
    19561 gtaaccgtct tatttatcgc agccgctggt ttggcctttg tgctgggtgg taatcaaaaa
    19621 aattatgcct ttgctgaaga tgcttctttt atcgtaagaa ctggggtaaa gatactgttt
    19681 ccccttatgt ttttactagg tatttatata atcgtacacg gacatttaac acccggtggc
    19741 ggtttccagg gtggggcagt aattgccact gccattttac tgaagttttt agcatataag
    19801 gactataagt taaaccatac tggtttatct ttactggaga gttttgccgg tatttcattt
    19861 ctaattattg gtttaattgg actattaaca aacggttcat ttttaactaa cttcttagga
    19921 aacggaactt taggaaccct cttttcagct ggtattatcc ccattatcta tatcttaatc
    19981 gggattaaag taggagcgga actatccggt attattagta acttgattaa tgcttaattt
    20041 taaaaattaa aaaggatgtg atactttggc ttttttaaac caatgggctt acatcggagg
    20101 ttcaataatc ttgatcttaa tcggtctttt tggggtcttg accagaaaaa atttgattaa
    20161 aattgttgtc agtatcggta tcatggatac cggtgttaac ctccttttgg ttagcttagg
    20221 ttatataaat aacggaatag ctccgatact cacagaagct aatgccagta atagtttaaa
    20281 ttttgtagac cctttacctc aggcattagt gctgactgcc atagtaatcg gagttgcggt
    20341 cttggcttta gcactttcga tagtcattaa aatttatgaa aatcaaaata ccctaaaaat
    20401 cgatgaaata agggggttaa aactgtgaat gcaagcttat taattgcagt ccctttactg
    20461 actgcctttg taataccatt aatcggtaag ttacataaga aaataccctc atttctggta
    20521 ttactaagta ctttatttaa tataataatt actttttcac tatatggcgc tgtaatggaa
    20581 agtccaattg taattcaaat cggaaattgg acggttcctt ttgggattaa cctatatgta
    20641 gataaattag ctttagttag tctaatgatc atttctacca ttacattttt aatttctatt
    20701 tataatataa gaaatacaga tgagttaaat gaaaccaaat tcaatacaat gttcctttta
    20761 gcctttacag ggattaatgg catcgtactg acgggagata tcttcaacct atttgtattt
    20821 atagagattg ctgcaatttc cacttatgta ctggctacca tgaaaagaaa agaaagctat
    20881 caggc
//
LOCUS       DATGCJ010000022        14078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_136742, whole genome shotgun sequence.
ACCESSION   DATGCJ010000022 DATGCJ010000000
VERSION     DATGCJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136742"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            943..1359
                     /locus_tag="VKN64_01015"
     CDS             943..1359
                     /locus_tag="VKN64_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HMA58840.1"
                     /translation="MKSVREYLWFNTNERVELINITRDVEKIVKESGIQEGMCLVNAM
                     HITASVFINDDESGLHNDYKKWLEELAPHEPISQYEHNTFEENADAHLKRQIMGREVV
                     VAITEGELDFGPWEQIFYGEFDGQRRKRVLVKVIGE"
     gene            1359..2126
                     /locus_tag="VKN64_01020"
     CDS             1359..2126
                     /locus_tag="VKN64_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HMA58841.1"
                     /translation="MLLAIQKIISSLVTLPGLFLVLNLFIFIYLLKTSWSTFMKLISA
                     LTIIIMVISFTGIGVHLMVVPLENYATEISDFSKSQIKNVPTVVLGGGIHYNVNGNQS
                     ELSSISRDRLLKGVLIARQNKMPLIYTGGVGVGYDEISEADVAQEFVNNFDNIEFIQE
                     KEAQTTYENGQEIKKWMQENQYDQIYLVTSAIHMRRSLGVFDNLNIDYIPVVSNYSYS
                     HNLSWLDYLPSRGCLRANMQAVHEWIGLLWYKINSRL"
     gene            2150..2977
                     /locus_tag="VKN64_01025"
     CDS             2150..2977
                     /locus_tag="VKN64_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA58842.1"
                     /translation="MSADLHIHTTYSDGSFTPAEIVDKAIEKGFNTIAISDHDTLDGI
                     KPAIDYAADKDIEVIPAIEFSTFRGKAEIHVLGYFIDFDDEKLKDKVRKIYDARKLRA
                     EKMVELLNKEGVEITFSEVREIAGDDYIGRPHIAQTMVKKGYIDEMGDAFTEKYIGNG
                     GRAYVKKYKISPEEAIELILESGGIPVLAHPIFINHGKPLREDQIKKLKDNGLLGVEV
                     YHSKHTAEDAKYYKNIAEKLELLITGGSDFHGENSPGVYLGDIRLADKHVKKLKEAR"
     gene            3064..4218
                     /gene="sat"
                     /locus_tag="VKN64_01030"
     CDS             3064..4218
                     /gene="sat"
                     /locus_tag="VKN64_01030"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HMA58843.1"
                     /translation="MIEPHGGTLVNKIVDNSSKEELLAAAEDMYHLALNERDLVEAEN
                     IATGLYSPIEGFMTEEDYKSVVENMRLSDGTVWTIPIVLGVDKNEADQIQEGKRIALT
                     DKSDNVYAVMDVEDIYEYNEKEEAELVYKTTSSEHPGVKNVYERGQYHLGGEIHLIRR
                     IEHGKFNQYRLDPKDVRAHIEAQGWDRVVGFQTRNPIHRAHEYLQKCSLEITDGLLLS
                     PLVGETKSSDIPVDYRIESYEVVMDKIYPQGRVFLSVFPAAMRYAGPREAVFHSLCRQ
                     NYGCTHFIVGRDHAGVGDYYGTYEAQEMFDNFSQDEIGITPLRFEYSFYCKKCGSMAT
                     TKTCPHDSENHIGLSGTKVRRLLREGKRPPEEMTRPEVADVLIRGMAETK"
     gene            4218..4793
                     /gene="cysC"
                     /locus_tag="VKN64_01035"
     CDS             4218..4793
                     /gene="cysC"
                     /locus_tag="VKN64_01035"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HMA58844.1"
                     /translation="MKVERSNNTMDKRAKGVTVWFTGLSGAGKTTVALEVEKQLKERG
                     YYTQRLDGDIVREKLTKDLGFTKEDRNKNIERNTFVAKILTDNDVITLCSFISPYQSA
                     RNYAREEISEVGEFIEVFVNTPLEVCEERDVKGLYKQAREGKIENFTGISDPYEEPEN
                     PDVELKTAQETVEESAEIVIDHLEEQGILPE"
     gene            4817..5053
                     /locus_tag="VKN64_01040"
     CDS             4817..5053
                     /locus_tag="VKN64_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HMA58845.1"
                     /translation="MGDKKATIKIPRNLYNNLNQIIKNSGFNSVTEFIVYVLRDLVSD
                     HKMNEEEISDSRQELSNEEVKMIKKRLKSLGYLD"
     gene            5123..5776
                     /gene="fsa"
                     /locus_tag="VKN64_01045"
     CDS             5123..5776
                     /gene="fsa"
                     /locus_tag="VKN64_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMA58846.1"
                     /translation="MKFFIDTANLEEIKTAESFGILDGVTTNPSLVSKEGDIDFHTRI
                     KEICEVVDGPVSAEVVSLDTEGMIEEARELAQLASNVVVKIPMTIDGLKAVKTLSKEG
                     IKTNVTLMFSANQALLAAKAGATFASPFIGRLDDRAHEGMDLIKNMRTIYDNYGYDTE
                     ILAASIRHPLHVKHAALAGADIATIPYEVIEKMSKHPLTDSGLERFLSDWKKFQEQK"
     gene            5893..6189
                     /locus_tag="VKN64_01050"
     CDS             5893..6189
                     /locus_tag="VKN64_01050"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HMA58847.1"
                     /translation="MRFLQKVIRIIQFFRDKNVPFHKKLLLALPLIYLIIPYDFIGDF
                     FPVMGQLDDIAVFVIMWPFLSSMMNEYQGPDNDGKDNIDKSDSIDLNKNDYEID"
     gene            complement(6282..9773)
                     /locus_tag="VKN64_01055"
     CDS             complement(6282..9773)
                     /locus_tag="VKN64_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HMA58848.1"
                     /translation="MSEEKSINLTQNALKVLKARYLKRNKEGELIEDPEGMFDRVAAN
                     IAEANKEYGEDVKKDREDFYNLMTSLDFLPNSPTLMNADNDLQQLSACFVLPVEDSMK
                     GIFNSLKNSALIHKSGGGTGFSFSRLRPKNDIVKTTGGVASGPLSFMKIFDKATDIVK
                     QGGKRRGANMGILRVDHPDIMDFITAKENEDMLNNFNISVAVTDEFMQAVEKDEFYEL
                     VNPHNNKVTAKLNAREVFEKIVYMAHKNGEPGIIFLDEINRHNPTPNVGEIESTNPCV
                     TGDTLVSTEDGLMRIDKLYEKYKEKGYKVITDSRIPAVLPKAVNSGNPVYSTNINKKG
                     TQVQEAVKIYNNGKKDVYKLTTKKGYEIKATNDHKFLTDKGWKELQDLKREDKVLIQS
                     DEGSFSNSSKLPFEVNNTVKGKNGKSYKFNFPQEWSEELGQVLGFLIGDGWLRKGDKN
                     CRVGFTFSKNDQQLLKYFKEILNQMYGKDIKPIKRKNDTYHLSYHSKFFVDFFNKLGV
                     KSTSSSSKEVPASFFTATKEAVSGFLQGLFTADGTVSNDKNKGNYYVRLSSKSLKLLK
                     QVQQLLINLGIKSRIYNRSRKASDNKFTYTNINGEKTNYKSDGILYELQISSGSLYTF
                     AEKIGFLLERKEKELTDIINNHNLHQQKMFDEIKNIEYAGKETVYDLTEPVTHSFIGN
                     GFVIHNCGEQPLLPYEACNLGSINLAHMTKKVNGDYRIDYNKLENTVRKAVRFLDNVI
                     DMNNHPLEQIEEMVQGNRKIGLGVMGWSDLLIRLRIPYNSEEAVKLGEDIMAFIEEVS
                     HDESSNRAKKRGPFPNIEGSIYDRPMRNATTTTIAPTGTISIIAGVSSGIEPLFAINY
                     ERHILDDVLIETHPIFEEIAKERGFYSEELMEKVAKNNSIQHMDEIPEDVKRIFVTAH
                     DIEPEWHIKMQAAFQKSVDNAVSKTVNFKNSATVDDVKEVYEKAYELNCKGVTIYRDG
                     SRSEQVLSTSSEEEQQAKHDRKTKRPRPKITYGTTEKTKIGCGKLYITINSDQKGINE
                     VFTTTGKGGGCHAQSEAISRLASLALRSGVAIEEVVDQLRGIRCEAAMIRKDVHNLSC
                     PDAIGRALEKYDEEEINKKFNMDIEVDETEQESSPQIDQEVDPYTNGSACPECGHELE
                     HEGGCSVCRNCGWSHCG"
     gene            9960..10439
                     /locus_tag="VKN64_01060"
     CDS             9960..10439
                     /locus_tag="VKN64_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMA58849.1"
                     /translation="MLSDKFNVIEMLEMAKEIEDRGYRLYSTHAKKTDDPKLKKIFNK
                     LASDEKDHYNTFDKLEKDYKDKDQKDYDYLEKVEVNDYLQSFVQFEVFPRGETEELED
                     METVEVLDRAIQSEKDSILLYRELIPYNEGETKEVLERLIEEEKEHYISLVNYKKEL"
     gene            10504..11640
                     /locus_tag="VKN64_01065"
     CDS             10504..11640
                     /locus_tag="VKN64_01065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMA58850.1"
                     /translation="MEMLLDLFSKKRKNQDILYRNFNFTFCLKLFLLMVLAGIFFDNP
                     ANSVNFSVYPLEEAVRLNLRPPVIILTGMILGPIWGAVAGGFIDISSFLIWHIDMNYM
                     FIFTLLTMLRGFISGYIFNFIFKRFSWKSVVTSIAFPHLLISGIIIPVALYYNYGVPL
                     INNVRIRLIIQIFTIPIFTVIFYYILKGMKKSKELKTLHSKLKEMLKIDDLTGLSNRR
                     HFMDFLNKMHSLSQRHGHSLSILMADIDNFKEINDNYGHHKGDQFLRAVGDILKRKIR
                     NEDLAARMGGDEFVILLPETSLKEALYIAHRIKKCVKNIDILSEKENSSISIGAAQLK
                     EDDDTESFLKRVDDALYQAKEKGRNRVETISIDPSQVTKELEFS"
     gene            complement(11626..12528)
                     /gene="murB"
                     /locus_tag="VKN64_01070"
     CDS             complement(11626..12528)
                     /gene="murB"
                     /locus_tag="VKN64_01070"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMA58851.1"
                     /translation="MDKLLNNFGKIQGLDVKQNVPLKEYTTFQVGGPADIFITPKHED
                     ALRDLTQLISEINKPYFILGKGSNIIVGDKGYRGIIIYTGHLDHINIEENEIKAQCGS
                     TLKDIAETAKNHSLTGMEFASGIPGSLGGAVFMNAGAYGGEMNDIIKKVTAYDQNGNK
                     KVMQKDELNLSYRDSFFQKKDYIIFDALLELQPGNKEKISAKMNKLNQKRKNKQPLEY
                     PSAGSSFKRPANHYTGPLIEKANMKGYQIGGAQVSEKHAGFIINKGNATAKDIVKLIQ
                     KIQQEVYQISGVKLKPEPKFLGEF"
     gene            12974..>14078
                     /locus_tag="VKN64_01075"
     CDS             12974..>14078
                     /locus_tag="VKN64_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMA58852.1"
                     /translation="MNNKVKIFLFIVFTIFIGFSLMIYNINTLENNIWNILFFTIILL
                     FLSNLSEFINFESSTSTSISLPVLIPAITILNPFLITFIAFIGTSEISFGKEKIPWYK
                     FFFNRCVIGLSAGTAAYIYRVSLNIITSNNLIALFFSSVIYFIINSGLVYAVIYLSGE
                     EQKISYRVFLNELFKSIFMSYFLGLILYFSYIQFGKLFFILVIILIYIARDIFFSRIQ
                     LLNSFTQVIESFLKVIDSKDHYTEGHCERVANYTKQLCDVAKVRHGRKERIVNMAKIH
                     DIGKIYVDDEILKSDEVLSKEEFAEMKKHSYYGYELLKDIDMLNDDLEIILHHHEFYN
                     GNGYPDGIEEKEIPLGSRILNICDSFDVMTTGRS"
BASE COUNT         4806 a         2060 c         2608 g         4604 t
ORIGIN      
        1 taattaaaat agattaagaa agaggggtga aaaaaatgag aaatacaaat caaaaatatc
       61 aaatgttaga aaacagaaat cagagaagag aaataaaatt aaacaagaga aacagaagac
      121 taactgacga gtacataaac caatacttct aaaataaaaa aattgaagcg aaaggaggct
      181 aaaaaatgtt tgcaaagata aatgaatttt tctcaattga agataaggat tataaaaagt
      241 taagaaaaga tcaacttcat cagaacagga aaatggcaga atacagaagc gaaattatgt
      301 aaataacttt taaaaataag aaaggagtgt ataaaaatgt taaaaaaatt aaatagtttt
      361 ttctcattag agagtaatga gtataaaaag tataataaag aaaggctgca tcaaaacaca
      421 agaatggaag aatatagaag cgagttaatg taaataatag attaaacaaa aagtaaaggg
      481 ggtagaaaaa tgttcaataa aattaatgat ttcttttcat tagaaaataa agactataaa
      541 aagttaagaa aagataaatt gcatcaaaac acaagaatgg aagaatacag aagtgaaata
      601 atataaccaa taaatttaaa ttaaaataag gaggtataaa tatagatgtc ggatttaaga
      661 atatatttaa aaaagaaatt ggaagaggat aaacagaata ataaaaataa ctgcataagg
      721 ataaaccaca ttaagatcag aaaaaaataa gagaatggag gtggaagata aatggtggtt
      781 atcaaaaaac cttatgtaga aaaattaaaa attaaaaagt ttgtttaaaa agtccctttg
      841 tataagaaat acaaaaaggg acttttttta atttaaggaa cttgttctta tatcctattt
      901 atcttataat ataataaata aaaaaattaa gaggtggtat tagtgaaatc tgttagagaa
      961 tatctatggt ttaatactaa tgagcgggta gaacttatta atattactag agatgtggag
     1021 aaaattgtca aagaaagcgg tattcaagaa ggaatgtgcc tggttaatgc catgcatatt
     1081 acggccagcg tatttataaa tgatgatgaa tccggtttac ataatgatta taaaaagtgg
     1141 ttagaagagt tagcacctca tgaaccgatc tcccagtatg agcataacac ttttgaagaa
     1201 aatgctgatg ctcatctaaa gagacagatt atgggacgcg aagtggtggt tgctattacg
     1261 gaaggggaat tagattttgg tccctgggag cagatttttt acggtgaatt cgacggtcag
     1321 cggagaaaaa gagtcctggt aaaggtgatt ggagagtaat gctattagcc attcaaaaaa
     1381 tcatatcttc cttggtgaca ttacctggcc tatttttggt tttaaatctt tttatcttta
     1441 tctatctttt aaaaactagc tggtctactt ttatgaagtt aatctcggct ttaacaatta
     1501 ttatcatggt catctccttt acgggaattg gggttcatct aatggtagta ccgcttgaga
     1561 attatgctac tgagatcagc gatttttcga aaagtcaaat aaaaaatgta cctacggttg
     1621 tattaggagg cggaatccat tataatgtta atggtaatca aagtgagctt tcttcaataa
     1681 gccgtgatag gttattaaaa ggtgttttaa tcgcccgcca gaataagatg cccttgatct
     1741 atacgggagg agttggcgta ggctatgatg agatcagtga agcagatgtg gcacaagagt
     1801 ttgtaaataa ttttgataat attgagttta ttcaggaaaa agaagcccaa acaacttacg
     1861 aaaacgggca ggaaattaaa aaatggatgc aggaaaatca atatgatcaa atttatttag
     1921 tgacttccgc aatacatatg cggagatcct taggggtctt tgataattta aatattgatt
     1981 acataccggt tgtatccaat tacagttaca gccacaactt atcctggctt gattatttac
     2041 caagccgggg ttgtctgagg gctaatatgc aggctgtgca tgaatggata ggactgttat
     2101 ggtataagat aaacagcaga ttataagata cagtagagga ggcagttaaa tgagtgcaga
     2161 tttacacatt cacacgacct attcggatgg aagttttaca ccggccgaga tagtagataa
     2221 agcaattgag aagggattca atacgattgc tatttcagac catgatactt tagatggtat
     2281 caagcccgct atagattatg ctgctgataa agatattgaa gttattccgg ctattgaatt
     2341 ttctactttc cgaggtaaag ctgaaatcca tgtattaggg tattttattg actttgatga
     2401 tgaaaaatta aaagataaag tccgcaagat ctatgatgca agaaagttaa gggcagaaaa
     2461 gatggttgaa cttcttaata aagaaggagt agagataact ttttcagagg taagagagat
     2521 cgcaggcgat gattacatcg gtcgaccgca tattgcccag accatggtca aaaagggcta
     2581 tattgatgaa atgggagatg cctttaccga aaagtatata ggtaacggcg gccgagctta
     2641 tgtaaaaaaa tataagatta gtccggaaga agcaatcgag ttaattttag aaagcggtgg
     2701 gatcccagtt ttagctcatc ccatttttat aaaccacggt aaaccgttaa gggaagatca
     2761 gattaaaaag ttaaaagata atggactttt aggggtagag gtataccatt ctaaacacac
     2821 agctgaagat gcaaagtact ataaaaatat tgctgaaaaa ttagagctgc ttatcacagg
     2881 cggatctgat tttcacggtg aaaactcccc gggagtctat ttgggtgata ttcgtttagc
     2941 tgataaacat gtaaaaaaat taaaagaagc aaggtaattt tttttgaaat aaaagaggat
     3001 tataggagtg tatcttgtat atattattag agtgttatta aacaggaggt attaaattta
     3061 ataatgatag aaccacatgg tggaacatta gtcaataaga tcgttgataa ttcaagcaaa
     3121 gaagaacttt tggccgcagc agaagacatg tatcatttag cattaaatga gagagattta
     3181 gttgaagctg agaatatagc aactggatta tacagtccta tcgaaggatt tatgacagaa
     3241 gaggactata agagtgtagt cgaaaatatg cgattatccg atggaactgt ttggacaatt
     3301 cccattgttt taggtgtaga taaaaatgaa gctgaccaaa ttcaagaagg aaagagaatt
     3361 gccttaaccg acaaatcaga taatgtatat gcagtaatgg atgtcgaaga tatctatgaa
     3421 tataatgaaa aagaagaagc ggaacttgtc tataaaacta cttcttcaga acatcccgga
     3481 gttaagaacg tatatgaaag aggtcaatac cacctcggtg gggagatcca tttaattaga
     3541 agaatagaac atggtaagtt taatcaatat agattagatc ccaaagatgt tagagcccat
     3601 attgaagcac agggctggga ccgggtagtt ggttttcaaa ctagaaaccc tattcatagg
     3661 gcccatgaat atctacaaaa gtgttcactg gagataacag acggattact actttcacct
     3721 ttagtaggtg agactaaaag tagtgatatt ccggttgatt atcggataga aagctatgaa
     3781 gtagttatgg ataagatcta tcctcagggt agagtattct tatctgtatt tccagccgca
     3841 atgagatatg caggtccacg tgaagcggta tttcattcac tctgccgcca gaattacggc
     3901 tgtacacatt ttattgttgg tagagaccac gcaggcgtag gggactatta tggtacttat
     3961 gaagcccagg aaatgtttga taacttttcc caggatgaaa taggtattac cccgcttaga
     4021 tttgaatatt ctttctactg caaaaagtgt ggttccatgg caactacaaa gacctgtccg
     4081 catgacagtg aaaaccatat aggacttagt ggtacaaaag ttagaaggtt attaagagaa
     4141 ggtaaaaggc cacctgaaga aatgactaga ccggaagtgg ccgatgtatt gattagagga
     4201 atggcagaaa ctaaataata aaagttgaaa ggagcaataa tacaatggat aaaagagcta
     4261 aaggagttac agtttggttt acaggactaa gtggagcagg aaaaacaacc gttgctctag
     4321 aagtagaaaa acagttaaaa gaaagaggat actatacaca gcgtttagac ggagatatcg
     4381 ttagagaaaa actaaccaag gacttaggat ttaccaaaga ggatagaaat aagaatatcg
     4441 agaggaatac ctttgtagca aaaattctaa ccgacaatga tgtaattaca ctttgttcct
     4501 ttatttcacc ctatcaatct gctagaaact atgcacgcga agaaattagt gaagttggag
     4561 aatttataga ggtgtttgtc aatacaccat tagaagtttg tgaagaaaga gatgttaaag
     4621 gactttataa acaggcccgt gaagggaaaa tagaaaactt tactggaatt tctgacccct
     4681 acgaggaacc agaaaatccc gatgtagaac ttaaaacagc ccaggaaact gtggaagaaa
     4741 gcgcagaaat tgttatcgat catctggaag aacaaggaat tttaccagag taaaaattta
     4801 agctggaggt attataatgg gagataaaaa agctacaatt aaaattccta gaaacctata
     4861 taataatcta aaccagatca ttaaaaattc aggctttaat agtgtaaccg aatttatagt
     4921 ctatgtatta agagatttag tttccgacca taagatgaat gaggaagaaa tcagcgattc
     4981 gcgccaggaa ttatcaaatg aagaagtcaa aatgataaaa aagaggttaa aaagtctcgg
     5041 ttatctggat taaaatttaa tattataatt agatatgata taatacttat caaataacaa
     5101 atacaaaagg tgggtgaatt atatgaagtt tttcattgat actgctaatt tagaagaaat
     5161 caaaactgct gaaagttttg gtattttaga cggtgttact acgaatcctt ctcttgtatc
     5221 taaagaagga gatatagatt ttcataccag gattaaagaa atttgtgaag ttgtagatgg
     5281 acctgtaagt gcagaagtag tcagtctaga cacggaagga atgattgaag aagctcgcga
     5341 acttgctcag ctggcttcca atgtagtagt taagattcct atgactatcg atggtttaaa
     5401 ggctgtcaaa acattatcta aagaaggtat taaaaccaat gtaactttaa tgttctcagc
     5461 caatcaggcc ctgcttgcag caaaagcggg tgcaaccttt gccagtcctt ttataggaag
     5521 actggatgat agggcccatg aaggcatgga cttgatcaaa aacatgagaa ctatctatga
     5581 taattatgga tatgatactg aaatcttggc tgccagtata agacatcccc tgcatgttaa
     5641 acatgctgct ttggctgggg ctgatatagc taccatacct tatgaggtta ttgagaaaat
     5701 gtcaaaacat ccactaaccg attctggttt agaaagattt ttaagtgact ggaagaagtt
     5761 ccaagagcaa aaatagattt gataaagata aataaagaac attaaaaccg gtggttttcc
     5821 accggttatt tttgtagaaa tcaactttat atattataat ataattatca gataaaaaaa
     5881 gaggtatata atatgagatt tttacagaaa gttatcagga tcattcaatt ctttcgagat
     5941 aaaaatgttc cttttcataa aaaattgctt ttagccctgc cgctaattta tttaatcata
     6001 ccttatgact ttataggtga cttttttcca gtcatggggc agttagatga cattgctgtg
     6061 tttgtaataa tgtggccctt tttaagcagc atgatgaatg aatatcaggg accggacaat
     6121 gatgggaagg ataatattga taaatctgat tcaatagatt taaacaaaaa tgattatgaa
     6181 atagattaga atttatttag aatccgaaag gattcttttt ttttattaaa ataaaaaagg
     6241 tgcccaaaat gggccccttt taactgcaag ttatttattt tttagccgca gtgtgaccag
     6301 ccgcagtttc tgcagactga gcaaccacct tcatgttcta attcatgtcc acattcagga
     6361 caggcagatc cgtttgtata gggatctact tcctgatcta tttgaggact gctttcttgt
     6421 tctgtttcat ctacctcaat atccatgttg aactttttat taatttcttc ttcgtcatat
     6481 ttctctaggg cacgtccgat cgcatctgga caggataagt tatggacatc ttttctgatc
     6541 atggctgctt cacatctgat accgcgtagc tgatcgacaa cttcttcgat ggcaacacca
     6601 cttcttaagg ccagggaagc aagtctagaa attgcttctg attgagcgtg acaaccgccg
     6661 cctttaccgg tagtggtaaa tacttcattg ataccttttt gatctgaatt aatagttata
     6721 tagagtttac cgcaaccaat ttttgtcttt tcagttgtac cataagttat tttaggacga
     6781 ggcctcttgg tttttctatc atgtttggcc tgttgttctt cttcactgga agttgagagg
     6841 acctgttcac tcctgcttcc atctctatag atagtaacac ccttacagtt taattcatac
     6901 gctttttcat atacttcttt tacatcatcg acggtagctg aatttttaaa gttaacggtt
     6961 ttgcttaccg cattatcgac tgatttttga aaagcggctt gcattttgat atgccattcg
     7021 ggttcaatat catgggcggt cacaaagatt ctcttgacat cttctggtat ttcgtccatg
     7081 tgttgtatag aattattttt agcgactttt tccattaatt cttccgaata aaagcctctt
     7141 tctttggcta tctcttcaaa aataggatgg gtctctatta aaacatcatc taagatgtgt
     7201 cgttcatagt tgattgcaaa taagggttcg ataccactgc ttactcctgc aataatacta
     7261 attgtgccag ttggagcaat tgtagtggtt gttgcgtttc tcatcggcct atcataaata
     7321 ctgccttcaa tgttagggaa gggccccctc tttttggccc tattagagga ttcatcatgt
     7381 gatacttctt ctatgaaggc cataatgtcc tcgcctaatt taaccgcttc ttcgctgtta
     7441 tatgggattc tgaggcgaat tagaagatca ctccagccca ttacacctaa acctatcttt
     7501 ctattaccct gaaccatttc ttcaatttgt tcgagtggat ggttgttcat atcaattaca
     7561 ttgtctaaaa atcttactgc ttttctcacc gtgttttcaa gcttgttata gtcaatccga
     7621 tagtcaccat ttaccttttt agtcatatgg gcaaggttaa tagaacctaa gttacaggct
     7681 tcatagggta aaagaggttg ttcaccacag ttatggataa caaagccatt gccgataaaa
     7741 gaatgggtaa ctggttctgt aaggtcatat acagtttctt tacctgcata ttcgatattt
     7801 ttaatttcat caaacatttt ttgctgatgc agattatggt tgtttataat atcggttaat
     7861 tccttttcct ttctttcaag gaggaaacct attttttcag caaaggtata aagcgaaccg
     7921 gaagatatct gcagttcata caggatacca tcggacttgt aattggtttt ttcgccgtta
     7981 atattagtat aggtgaattt attatcggat gcttttcttg atctattata aattctggac
     8041 ttaataccca gatttattaa aagctgctgc acctgtttta atagtttcaa tgatttggaa
     8101 gatagccgga catagtaatt tcctttattt ttatcattag atacagttcc atctgcagta
     8161 aatagcccct gtaaaaagcc acttacagct tccttggtag cagtgaagaa agaagcagga
     8221 acttctttgg aacttgaact tgtagatttt actcccagtt tattgaaaaa atcaacaaaa
     8281 aacttgctgt gataggagag atggtaagta tcattttttc ttttgatagg tttaatatct
     8341 ttgccgtaca tctggttaag tatttctttg aagtacttta ataattgctg atcattttta
     8401 ctaaatgtaa aaccaacgcg gcagttttta tctccttttc taagccagcc gtctccaatc
     8461 agaaatccca gaacctgtcc taattcttct gaccattctt gtggaaagtt aaatttataa
     8521 ctttttccat tttttccttt aacagtattg tttacttcaa agggtaattt agatgaatta
     8581 gaaaaactac cttcatcaga ttgtattagt actttgtctt ctctctttaa atcttgtagt
     8641 tccttccagc ccttatctgt gagaaattta tgatcattgg tagccttgat ttcataaccc
     8701 tttttagtgg ttaatttata aacatctttt ttgccgttat tatatatttt aacggcttcc
     8761 tgcacttggg tacctttttt atttatattg gtagaatata caggattacc actgtttact
     8821 gcttttggta gaacagcggg gatccgtgaa tcagttataa ctttataccc tttttccttg
     8881 tatttctcat ataatttgtc tatacgcatt aaaccatctt cagtactgac taaagtatct
     8941 cctgtaacac acggattggt gctttcaatt tctcctacat tgggggtggg attatgacga
     9001 ttaatttcat ctaagaaaat aatcccgggt tccccgtttt tatgagccat atatacaatc
     9061 ttttcaaata cttcccttgc atttaacttg gcagtaactt tattattatg aggattaacc
     9121 agttcataaa attcatcttt ttcaactgcc tgcatgaatt catcggtaac tgctacagaa
     9181 atattgaagt tatttaacat atcttcattt tctttagcgg taatgaagtc cataatgtca
     9241 gggtgatcaa ctcttaagat acccatgttg gcacctcttc ttttaccacc ctgttttaca
     9301 atatcagttg ctttatcgaa tattttcata aaagaaagtg gcccagaagc aacaccacca
     9361 gttgttttta ctatatcatt ttttggtctt aatctagaaa agctaaagcc ggtaccgcct
     9421 ccgcttttat gtattagggc ggagttttta agggaattaa aaattccttt catgctgtct
     9481 tctacaggta gtacaaaaca ggcactaagc tgttgtaaat cattgtctgc attcattaag
     9541 gttggtgaat tgggtaaaaa atctaaactc gtcattaagt tatagaaatc ttctctatct
     9601 tttttaacat cttccccgta ttctttgtta gcttcggcaa tatttgcagc aaccctatca
     9661 aacatgcctt cggggtcttc aattagttcc ccttctttgt ttctttttaa atatctagct
     9721 tttaatactt taagagcatt ttgtgttaaa tttatagatt tttcttctga cataaactca
     9781 cctcaaattt ctattattaa aattcgtgta tttaattata gcactaacta tctaaaaaat
     9841 aaaggtaagt agattagtta ttataatcac tttttaatct aaaaggagga tttaatatta
     9901 aattgtttaa tatagaaata agggtataat tataataata aaataaggag gaaatggata
     9961 tgttaagtga taaatttaat gtgatagaaa tgttagaaat ggctaaggaa attgaagata
    10021 gaggatatag gctgtattca actcatgcta aaaagactga tgaccctaaa cttaaaaaga
    10081 tatttaacaa actggcttct gatgaaaaag accattataa cacttttgat aaattggaga
    10141 aggattataa agataaagac cagaaagatt atgattattt agaaaaagta gaagtcaatg
    10201 attatctaca gagttttgtt cagtttgaag tatttcccag gggcgagaca gaagaattag
    10261 aagatatgga aacagttgag gtgttggata gagctatcca gtccgaaaaa gattcaatat
    10321 tactttacag ggaactaatc ccttacaatg aaggtgaaac aaaagaagta ctggaaagat
    10381 taattgaaga agaaaaagaa cattatataa gtctggttaa ttataaaaaa gaattataaa
    10441 attaaaataa gttaattaag caaactgaat gggaattttt tgacaaggac tgaaggtgta
    10501 ataatggaga tgttgttaga tttattttca aaaaaaagaa aaaaccaaga tatactatat
    10561 agaaacttca attttacttt ttgcttaaag ctatttctat taatggtttt agccgggatc
    10621 ttttttgata acccggctaa ttctgttaat ttttctgtat atccattaga agaagcggtt
    10681 cgcttaaact tgagaccacc ggtaataatt ttaacaggca tgattttagg tccaatctgg
    10741 ggagcagtgg caggtggatt tatcgatata tcttcttttt taatttggca tattgatatg
    10801 aattatatgt tcatctttac tttgctgaca atgttaaggg gctttatatc aggctacata
    10861 tttaacttta tatttaaaag atttagttgg aagtctgttg taacatctat tgcctttccc
    10921 cacttgctta tttcaggtat tataattcca gtagccttat attataatta tggagtccca
    10981 ttaataaaca atgtcaggat acgattgatt attcagatct ttactatccc gatttttaca
    11041 gtaatatttt attatattct aaaaggtatg aaaaaatcta aggaattaaa gactttacat
    11101 tctaagttaa aagaaatgtt aaagatagat gatctaactg gtttgtccaa ccggagacat
    11161 ttcatggatt ttttgaataa aatgcattct ttgtcacaaa gacatggaca ttctttatcg
    11221 attttaatgg ctgatattga taattttaaa gaaataaatg ataattatgg tcatcataag
    11281 ggggatcaat ttttaagggc tgtaggtgat attttaaaaa gaaaaattag aaatgaagac
    11341 ttagcggctc ggatgggagg agacgagttt gtaatactcc tacctgagac tagtctaaaa
    11401 gaagcccttt atattgcaca tagaataaaa aaatgtgtga aaaatataga tatactttcg
    11461 gaaaaggaga atagttcaat cagcatagga gcagcccaac ttaaagaaga tgatgataca
    11521 gaaagttttt taaagagggt tgatgatgcc ctttatcagg caaaagaaaa agggcgaaat
    11581 cgggtagaga caataagtat agatccgtct caagtaacaa aagaattaga attctcctaa
    11641 aaatttaggc tcgggtttaa gcttgacccc cgatatttga taaacttctt gttgtatttt
    11701 ttgaattagt ttaacgatat ctttggcggt agcattacct ttatttataa taaaaccggc
    11761 gtgtttttct gagacctggg caccaccgat ttgatagcct ttcatattgg ctttttcaat
    11821 taaaggaccg gtataatggt tggctggtct tttaaaagaa ctaccggcac tgggatattc
    11881 aagaggttgt ttattttttc ttttttggtt taacttgttc atttttgcac ttattttttc
    11941 tttattacct ggttgaagtt ctaataatgc atcaaaaata atgtaatctt ttttttgaaa
    12001 gaaactatct cgataagaga gatttaattc gtctttctgc ataacttttt tgttaccatt
    12061 ttgatcataa gctgtaactt ttttaatgat atcattcatc tcaccaccat aagcgcctgc
    12121 attcataaat acggcacctc ccaatgagcc cggaatacca ctggcaaatt ccataccggt
    12181 gagagaatgg ttcttagcag tttcagcaat atcttttaaa gtagaaccac actgtgcttt
    12241 aatttcattt tcttcaatat ttatatgatc taaatgtccg gtataaatta ttatacctct
    12301 ataacctttg tctccaacta tgatattact gcctttaccc agtataaagt aaggtttgtt
    12361 tatttcactg attagttggg ttagatccct taaagcatct tcatgcttgg gagttataaa
    12421 tatatcagca ggtccaccaa cttgaaaggt ggtatattct tttaaaggga cattctgttt
    12481 gacatctaaa ccctgaattt ttccaaagtt atttaataat ttatccatga attacacctc
    12541 taagatgaat tttatcataa aaatatatta ttaccaaatc ttattttcaa ttaaacttaa
    12601 gcccaatggt tgccattatt ggaaacataa agggaattgt ttaaaggatt tttcggattt
    12661 gtaaagaatt agtaataaga agagtttttt tgttaatatc tgatggcata tatattaatc
    12721 aaaaagtgta acaaattact agatgagcct aaaaaatgag cttagccgac cttcgtgtac
    12781 gtgacctagc ttagtcaccc tacacattaa ataaacgaag ggaggctata atcatgagaa
    12841 agtggtatgt tactatatta actggactat taacatttgc ttccacaatt gctgcgttag
    12901 cagatactgt tcactggaga taaataaaat agtatatgcc atcagatatt ttttatatca
    12961 ggaggattaa atgatgaaca ataaagtgaa aatatttttg tttatagttt ttactatttt
    13021 tattgggttt tcattaatga tatataatat aaatacatta gaaaataata tttggaatat
    13081 actttttttt acaatcatct tattatttct cagtaattta agtgagttta ttaattttga
    13141 aagtagtacc tctacttcaa ttagtttgcc agttttaatc ccagcaatta caattttaaa
    13201 tccatttctt attaccttta ttgcttttat aggaacaagt gaaattagtt ttggcaaaga
    13261 aaaaattcct tggtataaat tcttcttcaa taggtgtgta ataggtttat ctgctggaac
    13321 agcagcatat atatatagag tttcattgaa tattataact tcaaataatt taattgcttt
    13381 atttttttca tcagtaatat attttataat aaatagtgga ttagtttatg cagtaattta
    13441 tttatctggt gaagaacaaa aaatatctta tagagtattt ttaaatgaac tatttaaaag
    13501 tatatttatg tcttattttt taggcttgat actttacttt agttatattc aatttggaaa
    13561 attatttttc attttggtta taatattaat ttatattgct agagacatat ttttttctag
    13621 aattcagctt ttgaattctt ttacacaagt aattgaaagt ttcttgaagg taatagattc
    13681 caaggatcat tatacagaag ggcattgtga aagagtagcg aactatacaa agcaactttg
    13741 tgatgttgct aaagttcgcc atggaagaaa agaacgaatt gtaaacatgg ctaaaatcca
    13801 tgatattggt aagatttatg ttgatgatga aatattaaag agtgatgaag tgttaagtaa
    13861 agaggaattt gctgaaatga aaaaacacag ttattatggt tatgaacttt taaaggacat
    13921 tgatatgttg aatgacgatc tcgagataat cctccatcat catgaatttt ataacgggaa
    13981 tggttatcct gatggaattg aagaaaagga gattccgtta ggttcaagaa tattaaatat
    14041 atgtgattca tttgatgtta tgactacggg acgcagtt
//
LOCUS       DATGCJ010000023         6731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_2876, whole genome shotgun sequence.
ACCESSION   DATGCJ010000023 DATGCJ010000000
VERSION     DATGCJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VKN64_01080"
     CDS             complement(<1..795)
                     /locus_tag="VKN64_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMA58853.1"
                     /translation="MNNIYVLDTNVLLDDPTAIFDYEEHEIIIPLAVVEELDNQKRKT
                     SDIGYNARETSRILDELRLKGRLNEGIKLKNGSILKIVIDKKELKLPPHLSASKYDNR
                     ILTTAIDLQESNPEKEVIMVSNDINLRLIANAFDLKAEDHRGNGIDGEELYDGFIEIK
                     TKANIIDQFYENQELDPEQLDGDFENLYPHQMIQLSTLDERSKSALARFDGKKIVPLI
                     FDKKAPMGITPLNREQKFAYELLLNDDIRIVTMSGKAGTGKTLLALA"
     gene            complement(814..1233)
                     /locus_tag="VKN64_01085"
     CDS             complement(814..1233)
                     /locus_tag="VKN64_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HMA58854.1"
                     /translation="MLKEYKTGQAFMGRLEKDDDLLNSLTEIVKEKDITTGFIKVIGA
                     VQNAKIGFYNQETYEYQYKNIDREMEIVSCIGNISILDGEPFIHAHITLADEDGSCYG
                     GHLAEGTTVFASEFFIQPLQGEKLERKYDEKTQLTLW"
     gene            complement(1235..1972)
                     /locus_tag="VKN64_01090"
     CDS             complement(1235..1972)
                     /locus_tag="VKN64_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA58855.1"
                     /translation="MFKTKKIVLLTFTFILLFSLSIQAQSLLQMGINEYNSGNYKKAI
                     DHLTSYAAENEGSYLPNFYLGLSYTKLDQFDLAIDSFNYARKVKGDSYNLLVNLGRAY
                     YNNNQKDEAYDVLIKAEGYKPVDEQLYNLLGLIHMDKREYSQAIKKFEMAIEEEQENF
                     YVYNNLSLCYIHLGNFSEARKNINKALDLDPTVPYVYNNAGIIYENLQQYEEAAEHYQ
                     QALEIDPNYKKSEVNLERVKDKMPEEE"
     gene            complement(1977..2615)
                     /gene="nth"
                     /locus_tag="VKN64_01095"
     CDS             complement(1977..2615)
                     /gene="nth"
                     /locus_tag="VKN64_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMA58856.1"
                     /translation="MENNVEKMIDYFEKKYPDPETALNYETPFQLLIATIMSAQTTDV
                     QVNKVTKDLFSKYKSPNDFAELEVRELQKEIQSIGLYKNKSKYIIESSKMIVEDFDGK
                     VPTNRKDLVKLPGVGRKTANVVLANAFDRETIAVDTHVFRVANRLGLVDTDKRDQVEQ
                     DLMEVVPENYWTKFHHWLIFHGREICKARNPLCDKCEIKSYCDYYQNQIKGG"
     gene            2726..3301
                     /locus_tag="VKN64_01100"
     CDS             2726..3301
                     /locus_tag="VKN64_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58857.1"
                     /translation="MNIGIDIDGVITHEKKGKENVWLRALNNYFEDQITRKKDVYNFT
                     EAYDIPLEDLKEFLRNKLHEIYSGVEIAPGAKEIIDKIKSTGHTIYLITARHQKFRPL
                     TIKWLEQHHVNYDYLYHEDNKAKLAVEKNIKLFIEDNQSNTIDLLKEDIPVVLVDKYH
                     NKEVPDRDILYRVDNWNQIKDILKKYDFLSE"
     gene            complement(3322..4971)
                     /locus_tag="VKN64_01105"
     CDS             complement(3322..4971)
                     /locus_tag="VKN64_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HMA58858.1"
                     /translation="MSTVVFNDTEYNISHNMKVLDLLSEIDIEDKESIIGVKVNNRLL
                     DLNYVIDQDCQLEFVRNDTPAGNRFYKRSLFMLMAKAVYEIYGDEILNIEHSLSNGIY
                     CELSDNNVLSKAELEDILNKMQEIVKKDYTINKHMMESEKGLEILRGQGNEEKSRIIE
                     QRKLDEIKLYELDGYYNYYYYPLVPSTGYLKNFKLHYHFPGFVLLYPHFDQPDKIEEF
                     IPQPKLADIFYDYEKLGDILEVGFVPDLNDHIEENNYGELIRISEGLHEKNIAQIADE
                     IASHKELKRIILIAGPSSSGKTTFTKRLSTQLRINGLRPVTISTDDYFVNRDKTPLDE
                     DGNFDFESLHAINLELFNDHLVRLMRGEEVRLPKFNFKKGVREDSDTVLKLSSDQPIL
                     IEGIHSLNNKLTEFIPEDHKYKIFVSALTQLNIDKQNRIPTSDTRLVRRIVRDNLFRG
                     HSALNTLEMWGRVRKGEEENIFPYQENADVMFNSALIYELAVLKNCAVPLLEEIKKDN
                     PYHYEARRLLEVLDCFKNMPSDDIPKTSILREFIGGSVYRK"
     gene            complement(4976..6049)
                     /gene="queG"
                     /locus_tag="VKN64_01110"
     CDS             complement(4976..6049)
                     /gene="queG"
                     /locus_tag="VKN64_01110"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMA58859.1"
                     /translation="MKIGNEIKRKFKEIGFDLIGFTKPVLDQDLVNNFKKRYRDDDLP
                     PFVHNDVGSILNPNQLNPWSKTVIVLGLSYAQNEESDTDGFISRYTRGSDYHLVMENK
                     LKKGIRYLKDLFDDLKSAYYVDNGPVLEKVLAQQAGLGWIGKNTLLVNEKFGTYIFLG
                     EIFINKSLDYDEKSENKCGDCTACLENCPTNSLSTPYYLNYRTCRSNLTQQKGYLEEK
                     EEKLIGNCIWGCDDCQTACPYNENIPRGLHEEFQPKIKGDIIKILKFNKKNFPKKWLN
                     KALSWRGMRIIQRNALIAINNLGIKNEQYKEVLIKKIEDPSPIIRYYAYKAYINLDFN
                     LDLIEKQIKREKEIDIHNIIKRK"
     gene            complement(6117..6224)
                     /locus_tag="VKN64_01115"
     CDS             complement(6117..6224)
                     /locus_tag="VKN64_01115"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetS family NSS transporter small subunit"
                     /protein_id="HMA58860.1"
                     /translation="MSGSAVVMLIFACVVLYGGLFVTLRKALKSRKKED"
     gene            complement(6254..>6731)
                     /locus_tag="VKN64_01120"
     CDS             complement(6254..>6731)
                     /locus_tag="VKN64_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HMA58861.1"
                     /translation="IVEAGVAALKDKWGGTKRTVTLFYSIFLFVVGLLFATKGGLYWL
                     DIVDHYVNTYGLVVAGLAESVIIGWYYDPNELRGYFNSVSEYQFGSWWNVMIKYVTPI
                     MLVVLLVNEFIADIGTQYGGYELRYLIYGGWAGVIGLLLVASFILSRQRGLDDAEI"
BASE COUNT         2113 a         1304 c          922 g         2392 t
ORIGIN      
        1 cgctaaagca agtaaggttt tgcctgttcc ggctttaccc gacatcgtaa caatccgaat
       61 atcatcattt aataatagct cataggcaaa tttttgttcc ctatttaagg gagtgatgcc
      121 catcggtgct tttttatcaa aaatcagagg aactatcttc ttaccatcaa accgggctaa
      181 agcactctta cttctctcat ccagggttga taattgaatc atctgatggg gataaaggtt
      241 ttcaaaatca ccatctagtt gctctgggtc taattcttga ttttcataga attgatcaat
      301 aatattggcc ttagttttaa tctcaataaa accatcgtat aactcttcac catcaatacc
      361 attacctcta tgatcctctg cttttagatc gaaggcattt gcaattagtc ttaaatttat
      421 atcattagat accattatta cttctttttc aggattagat tcctgcagat caatagccgt
      481 agttaagatc cgattatcat acttactagc acttaagtga gggggtagtt ttagttcttt
      541 tttatctatt actattttta aaatgctacc gttttttaat tttatccctt catttaatct
      601 gccttttagc cgtaactcat ctaaaatacg ggaggtttct ctggcattat accctatatc
      661 tgaggtcttt cgcttttggt tatctaattc ttctactaca gctaaaggta taataatttc
      721 gtgttcttca taatcaaaaa ttgctgtagg gtcatcaagt aaaacattgg tatctaaaac
      781 atatatatta ttcatattag gctcctttcc tgattaccag agggtcaatt gggtcttttc
      841 atcatacttt ctttctaatt tttcaccttg taaaggttga ataaaaaatt cactggcaaa
      901 aacggtggta ccttcggcta aatggccgcc ataacaactt ccatcttcat cagctaaagt
      961 tatatgggca tggataaatg gttcaccatc cagaatagaa atattaccta tacaacttac
     1021 gatctccatc tccctatcta tatttttata ctggtattca taagtttctt gattgtaaaa
     1081 accgattttg gcattctgta cagccccgat tacttttata aaaccggtgg taatatcttt
     1141 ttcttttacg atttcggtta aactatttaa aaggtcatcg tctttttcta atctacccat
     1201 aaatgcttgt cctgttttat attcttttag catgctattc ctcctcaggc attttatctt
     1261 ttactctttc taaattaacc tcagatttct tataattagg atctatttct aaggcctgct
     1321 gataatgctc ggcagcctct tcgtactgct gtaagttttc ataaattata ccggcattat
     1381 tataaacata aggaacagtg ggatctaaat ctaaggcttt attaatattc tttctggcct
     1441 cactaaagtt acctagatgg atataacata aacttaagtt attatataca taaaaatttt
     1501 cttgttcttc ttcaatcgcc atttcgaact tttttatagc ttgactgtac tctcttttat
     1561 ccatatgaat taaacctaat aggttatata actgttcatc gaccggttta tagccctctg
     1621 ctttaattaa aacatcatag gcttcatcct tttggttgtt attataatag gcccgtccta
     1681 aatttactaa gagattatat gagtccccct taactttcct tgcgtaatta aaagaatcaa
     1741 tggccaaatc aaattggtcg agtttggtat aacttaaacc taaataaaaa tttggtagat
     1801 aactgccttc attttcagcg gcataacttg tcagatggtc tatcgctttt ttataattac
     1861 cgctgttata ttcattgatc cccatctgta aaaggctctg tgcctgtatt gagagagaaa
     1921 ataataaaat gaatgtaaat gttaataaaa ctatcttttt tgtcttaaac accattttaa
     1981 cctcctttta tttggttttg ataataatca caataacttt ttatttcgca tttatcacag
     2041 agtggatttc tcgctttaca aatttctcgt ccgtgaaata tcaaccaatg atgaaacttc
     2101 gtccaatagt tttcagggac aacctccatg aggtcctgtt caacctgatc tcttttatca
     2161 gtatcgacta gtcctagacg attagcaact ctaaatacat gtgtatcaac cgcaatcgtt
     2221 tcgcggtcaa aggcattcgc taaaactaca ttggccgttt ttcttcctac ccccggtaat
     2281 tttactaggt cttttctatt ggtgggtacc ttgccatcaa aatcctctac aatcatctta
     2341 ctgctctcga tgatatattt acttttattc ttatatagac cgatcgattg aatttctttt
     2401 tgcagttctc tgacctctaa ttcagcaaaa tcattggggg atttatactt actaaagaga
     2461 tctttagtaa ctttatttac ctgtacgtct gtggtctggg ccgacatgat agttgcaata
     2521 agaagttgaa aaggagtttc ataatttaaa gccgtttccg ggtccgggta ttttttttca
     2581 aaataatcta tcattttttc aacattgttt tccaaattac ctctcctttc tggaaatctt
     2641 accaaattaa tgttaaaata atttttagta tctaaaaaaa ttatatcata atataggcat
     2701 atattgaaat ttaggaggta taattatgaa tattggaata gatattgatg gtgttatcac
     2761 acatgaaaaa aaaggtaaag aaaatgtctg gttaagggct ttaaataact attttgaaga
     2821 ccagatcacc cgtaaaaaag acgtctacaa ttttacagaa gcatatgata tacccctaga
     2881 agatctcaaa gaatttctaa gaaataaact gcacgaaata tattctgggg ttgagatagc
     2941 ccccggggca aaagaaataa ttgataaaat aaaatcgact ggacatacca tttatctgat
     3001 tacagcccgt catcaaaagt ttagaccctt gaccataaaa tggttagaac aacaccatgt
     3061 taattatgat tatttatacc atgaagacaa taaagcaaaa ctggctgttg aaaaaaatat
     3121 caaactattt atagaagaca accagtccaa tacaattgac ctcttaaaag aggatattcc
     3181 agtcgtcctg gtcgataaat atcacaataa ggaagtacca gatcgtgata tactctaccg
     3241 ggttgataac tggaatcaaa ttaaagatat attaaaaaaa tatgactttc tttctgaata
     3301 acatctaagt taatccataa attatttcct gtatacactc cccccgataa actccctcaa
     3361 aattgatgtt ttggggatgt catcagaagg catattttta aaacagtcca gtacttctaa
     3421 taaccgcctt gcttcataat gataaggatt atctttttta atctcttcaa gtaagggaac
     3481 agcacagttt tttaatactg ctaactcata aattaaagca gagttgaaca ttacatctgc
     3541 attttcttga taggggaata tattttcttc ttcccctttt ctcacccttc cccacatctc
     3601 taaggtatta agggcagaat ggcccctaaa taaattgtct ctcacaattc ttctcaccaa
     3661 acgggtatca gaagtgggta tcctgttttg cttatcaata tttaactggg ttaaggcact
     3721 tacaaatatc ttgtatttat gatcttccgg aataaattca gttagcttat tatttaaact
     3781 atggatacct tcaatcaaga tcggctgatc agaactcaac tttaaaaccg tgtcactatc
     3841 ttctctcaca ccttttttga agttgaattt aggcagtcta acttcttcgc ccctcatcaa
     3901 cctaaccaaa tgatcattga acagttccaa attaatcgca tgtaaagatt caaaatcaaa
     3961 gttaccgtct tcatctaaag gtgttttatc ccgatttaca aagtaatcat cagttgaaat
     4021 agttaccggt cgcaaaccat ttatgcgaag ttgggtagat aatcttttgg taaaagtggt
     4081 tttaccggaa gaagaaggtc cggcaattaa gataatcctc tttaattcct tatgagaggc
     4141 aatttcatct gctatttggg caatattttt ttcatgtaaa ccttccgaaa tgcggattaa
     4201 ttccccgtaa ttattctctt caatatgatc atttaaatcc ggtacgaaac ccacttccag
     4261 gatatcacct agtttttcat aatcataaaa tatatcagcc aattttggtt ggggaataaa
     4321 ttcttcaatc ttgtcgggtt gatcaaaatg gggatatagt aagacaaaac ccggaaaatg
     4381 gtaatgtaat ttaaagtttt ttaaatagcc tgtactcggt actaaaggat aataataata
     4441 attataataa ccatcaagtt catataactt aatttcatct aacttcctct gttcgattat
     4501 cctgctcttt tcttcattac cctggcctct taaaatctcg agcccttttt cactttccat
     4561 catatgttta ttgatcgtgt agtctttttt aacgatttcc tgcatcttat ttaatatatc
     4621 ttctaactcg gctttgctta agacattatt atcacttaat tcacagtaga taccattgct
     4681 taaggaatgt tcaatattta atatttcatc tccatatatt tcataaaccg ccttagccat
     4741 taacataaac aaacttcttt tataaaatct attaccggct ggagtatcat ttctgacaaa
     4801 ttcgagctga caatcctgat caattacata gtttaaatct aataatctgt tattaacttt
     4861 aactccaata attgactctt tatcctctat atcaatttct gataacaaat ctaaaacttt
     4921 catgttatga gaaatattat attcagtatc attaaataca acagtagaca ttacatcact
     4981 tcctcttaat tatattatga atatcaattt ccttttctct tttgatctgc ttttcaatta
     5041 gatctaaatt aaaatccaaa tttatatatg ctttataagc ataatatcga ataatcgggg
     5101 aaggatcttc aattttttta attaaaactt ctttatattg ctcattttta attcctaaat
     5161 tattaatagc tatcaaggca tttctttgaa ttatccgcat tccccgccag cttaacgcct
     5221 tatttaacca ttttttaggg aagttctttt tattaaactt caatatttta atgatatctc
     5281 ctttaatttt tggttgaaac tcttcatgaa gcccccgcgg aatattttca ttataaggac
     5341 aagcagtttg acaatcatca caaccccaaa tacagttacc aataagtttt tcctctttct
     5401 cttctaaata gcccttttgt tgagttaaat tactcctaca ggtcctgtaa tttaaataat
     5461 aaggggtact tagagaatta gtaggacagt tttcaagaca ggctgtacag tccccacatt
     5521 tattttctga tttttcatca taatctaaac ttttatttat aaatatttct cccaaaaaaa
     5581 tatatgtacc gaacttttca ttaactaata aggtgttttt acctatccag cctaaaccgg
     5641 cctgttgggc taaaactttt tctaagaccg gtccgttatc tacatagtaa gcacttttta
     5701 aatcatcaaa taagtctttt agatatctta tacctttctt aagtttgttt tccattacta
     5761 aatgataatc agaacctctt gtatagcgag atatgaaacc atcggtatca gattcctcat
     5821 tttgggcata agaaagccct aatacaatca cagttttaga ccagggattt aattggttcg
     5881 gattcaaaat acttcccaca tcattatgga caaaaggagg gagatcatca tccctatatc
     5941 tctttttaaa attattaact aagtcttggt ctaaaactgg ttttgtgaaa ccgatcagat
     6001 caaaacctat ctctttaaat tttcttttta tttcattacc tatcttcata tatatcactc
     6061 gttttctatt aacttaaaaa aggggccgaa aactcggccc ctttatgttt aattaattag
     6121 tcttcttttt tacgggactt taatgccttc ctcaaggtaa caaaaagtcc tccatacaaa
     6181 actacacaag caaatatcaa cattacaact gctgatccac tcataaaatt actcccttct
     6241 aaatacttat tttttagatt tctgcgtcat ctaaacctct ttgacgtgat aggataaatg
     6301 aagcaacaag taataatcca attactccag cccagccgcc gtaaatgaga tatctaagtt
     6361 catatccacc gtactgtgta ccaatatcag caataaattc attgactaat agcacaacta
     6421 acataatcgg tgttacatat ttgatcatca cattccacca gctgccgaac tgatattcag
     6481 agactgagtt gaaatatccc cttaattcat tgggatcata gtaccagcct ataattactg
     6541 actcggctaa accagccaca acgagtccat aagtatttac ataatgatct acaatatcta
     6601 accagtaaag tccaccttta gtagcaaata atagtcctac tacaaatagg aatatactat
     6661 aaaatagtgt aactgtcctt ttggttccgc cccatttatc tttaagtgct gctacaccgg
     6721 cttctacaat g
//
LOCUS       DATGCJ010000024         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_315847, whole genome shotgun sequence.
ACCESSION   DATGCJ010000024 DATGCJ010000000
VERSION     DATGCJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315847"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /gene="ggt"
                     /locus_tag="VKN64_01125"
     CDS             complement(<1..1601)
                     /gene="ggt"
                     /locus_tag="VKN64_01125"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HMA58862.1"
                     /translation="MKQDYLNYPYSSKQVTRFAKNGMVATSQPLAAQAGLDILKKGGN
                     AVDAAVATAACLTVVEPTSNGIGGDAFAIVWIKDEMHGLNSSGYSPQDITIKKVKEMG
                     YDEMPKTGWLPVTVPGAPAAWSSLVNRFGNLEMTEVLKPAIKYAREGYSVSPTLSKFW
                     KRAHKKYKEMLEGKQFAAWFDTFTVDGKPPEAGQVWSSNAHADTLQLIAESNSEAFYR
                     GELADKIDQFAKEYDGFLRKADLNAYQPEWVDPISVNYHGYDVWEIPPNGQGMVALMA
                     LNILNGFDFETKDTLETYHKQIEALKLAFSDGKKYITDIDKMKVKVEDLLSKAYASKR
                     RSLIKETALQPEPGNLDKGGTVYLAAADKEGNMVSYIQSNYMGFGSGLVVPETGIALQ
                     NRGHSFSLDKNHHNHLEPHKKPYHTIIPGFLSKNNIPIGPFGVMGGFMQPQGHLQVLM
                     NLIDFDLNPQAALDAPRWQWTEGKKIKVESDFPKHIAQALQRKGHEVSLSLDSGSFGR
                     GQIIFRDHETGVLKGGTEKRTDGHIA"
     gene            1847..2647
                     /locus_tag="VKN64_01130"
     CDS             1847..2647
                     /locus_tag="VKN64_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMA58863.1"
                     /translation="MHLKTKKFVIYTYFFTWFFWLILGITANYFGYSYGDPLYLFLFI
                     MGGLGPFYVVFIVYNPQKELRMYKRIKRRICKWNVSIRWYCWIFVIPFLIFLIPWVLN
                     SFVFKNSMILFRQNVLTVVPLILVNIVMGGLEEVGWRGVLLPGLLKKYNKISATIMTS
                     LIWSGWHLPLWFIEGSPQKGMNFIIFIILGLVFSFQLTIIYIKTKSLFLCIIMHSIFN
                     SYPGIINIFSDRIYLSTTAMLLFSFLIFIIIYPKEVGLKIKALNNTRF"
     gene            2679..>2863
                     /locus_tag="VKN64_01135"
     CDS             2679..>2863
                     /locus_tag="VKN64_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA58864.1"
                     /translation="MVNKKLKVGIIGCGDIANYKHMPALKELSDLCEMTSFCDIRINR
                     AVKAAEEFGIKDSKVYE"
BASE COUNT          856 a          509 c          486 g         1012 t
ORIGIN      
        1 gctatatgac catcggttct tttttcagta ccacctttta aaacaccagt ttcatggtct
       61 ctaaagataa tctgtcctct gccaaaactg ccagaatcta aggataaact aacttcatgt
      121 cctttgcgtt ggagtgcctg agcaatgtgc tttggaaaat cactttcaac ctttatcttt
      181 ttaccttcag tccactgcca tctgggtgca tcaagagcag cctgaggatt caaatcaaaa
      241 tcaattaagt tcattaaaac ttgtaggtgt ccctggggct gcataaaacc tcccatcaca
      301 ccaaaaggcc cgataggtat attgttctta cttaaaaagc ccggtatgat tgtatggtat
      361 ggttttttat gaggttctaa atgattatgg tgatttttat ccagggaaaa actgtgtccc
      421 ctattttgta gggcaatgcc ggtctcagga actaccagtc cggaaccaaa gcccatataa
      481 ttactttgaa tataggagac catatttcct tctttatcag cagcagccag atagacggtt
      541 cctcccttat ctaaattacc aggttcaggt tgtaaggcgg tttctttgat caaactacgc
      601 cttttagaag cataagcttt tgagagtaaa tcttccacct ttactttcat cttatcaata
      661 tctgtaatat atttttttcc atctgaaaaa gccaatttta aggcttcaat ttgtttatga
      721 taggtttcca atgtatcttt tgtttcaaag tcaaaaccgt ttaatatatt caaggccatt
      781 aaagcaacca ttccctggcc attgggagga atttcccaaa catcataacc atggtagttg
      841 acacttatag gatcaaccca ttccggttga tatgcattta aatcggcttt ccttaagaaa
      901 ccatcatatt cttttgcgaa ttgatctatt ttatctgcta attcccctct atagaaggct
      961 tcactattac tttccgcaat taattgtagt gtatccgcat gagcatttga actccatact
     1021 tggcctgctt ccggtggttt accatctacc gtaaaagtat cgaaccaggc tgcaaactgc
     1081 tttccttcta acatttcttt atatttctta tgtgcccttt tccaaaactt acttaaagta
     1141 ggagaaacgg aataaccttc ccgggcatat ttaatagcgg gttttaaaac ttctgtcatc
     1201 tcaaggttgc cgaatctatt aacgagactg gaccaggcag ccggggcacc gggaactgta
     1261 accggcagcc aacccgtttt cggcatttca tcataaccca tttctttaac ctttttaata
     1321 gttatatctt gtggagaata tccgctggaa tttaatccat gcatttcatc tttaatccag
     1381 acgattgcaa aagcgtctcc cccgatccca tttgaagttg gttctactac cgttaaacag
     1441 gcagcggtag ccacagctgc atctacagca tttccacctt ttttaagtat atccaggccg
     1501 gcctgagctg ctaacggttg agaagtcgct accatgccgt ttttagcaaa tctagtaacc
     1561 tgtttagagg aataaggata gttcaaataa tcttgcttca tattaccact ccattctcaa
     1621 tttttaaaga ccttatttaa ttatatttca tttttcctat gatatcaaaa ataatgccta
     1681 ttttctatct ttaagagaga aataatttat aaattcctat ttagatttat aaataaacaa
     1741 ggttaattat aattaaattt tcctgtaatt aaaggaatat atttctattt caataatata
     1801 taaattaatt gaatagaata ttaattttat aaaggagtga cattctttgc atcttaaaac
     1861 aaagaagttc gttatatata catatttttt cacctggttt ttctggttaa tcttaggtat
     1921 aactgccaat tattttggtt atagttatgg tgacccctta tatttatttc tttttattat
     1981 gggtgggtta gggccttttt atgtagtttt tatcgtttat aaccctcaaa aagagttaag
     2041 gatgtataag aggataaaaa gaagaatctg caagtggaat gttagtatca ggtggtattg
     2101 ttggattttt gtaattccct ttttaatttt tttaataccc tgggttttaa atagttttgt
     2161 atttaaaaat agtatgatac tttttaggca gaacgtattg actgtggttc ctttaatttt
     2221 agtcaatatt gtaatgggag gtttagaaga agtaggttgg cgcggggttt tattacccgg
     2281 cttattaaaa aagtataata agatatctgc aaccataatg acaagtctaa tctggtccgg
     2341 ctggcacttg ccgctctggt ttatagaggg ttcgccccaa aagggtatga attttattat
     2401 ctttataatt ttaggattag ttttttcctt tcaacttaca ataatttata ttaaaactaa
     2461 aagccttttt ctttgtatca taatgcacag tatttttaat tcatatccgg gtataattaa
     2521 tatcttttca gatcgaatct atttatcaac tactgcaatg ctattatttt catttctcat
     2581 cttcatcatc atctacccga aagaagttgg tttaaaaatt aaagctttaa ataatacccg
     2641 gttttaaata tataagaagt tagaaggggg agttaagagt ggttaataag aaattaaagg
     2701 tgggaataat aggttgtgga gatattgcaa attataagca catgcctgcc ttaaaagaat
     2761 tatcagattt atgtgaaatg acatcttttt gtgatataag gattaataga gctgtcaaag
     2821 cggccgagga gtttggtata aaagattcta aagtatatga aga
//
LOCUS       DATGCJ010000025         9702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_449670, whole genome shotgun sequence.
ACCESSION   DATGCJ010000025 DATGCJ010000000
VERSION     DATGCJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449670"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VKN64_01140"
     CDS             complement(<1..404)
                     /locus_tag="VKN64_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HMA58865.1"
                     /translation="MRYKKKRLIKIAFIIAIVALAVFLYFNNSINLGLDLQGGSHIVL
                     QAQPTEERDITNEVMSGIQQVIERRVNQLGLTEPVIQREGDDRIIVELPAVDNPNEAI
                     ETIGRTAVLTFRNPAGQVLMTGEFVKNATADY"
     gene            complement(442..981)
                     /gene="lepB"
                     /locus_tag="VKN64_01145"
     CDS             complement(442..981)
                     /gene="lepB"
                     /locus_tag="VKN64_01145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMA58866.1"
                     /translation="MDSGDVKEILQSVVIAGILAFLIITFVAQSFVVDGKSMEPNLHD
                     GERLFVNKFIYRFRDPQRGEVVVFTPRGAPNKKYIKRVIGLPGDTVYIKDGVTYVNGQ
                     PLEEDYIKEDMKGNFGPYQVPEKSVFVMGDNRNNSADSRYPGYVGYVPYETISGLAFW
                     VYWPITEMRIIDQVEYENL"
     gene            complement(1052..1357)
                     /gene="yajC"
                     /locus_tag="VKN64_01150"
     CDS             complement(1052..1357)
                     /gene="yajC"
                     /locus_tag="VKN64_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMA58867.1"
                     /translation="MQYVYAILPWVLIFGVFWFFLIRPQRKQQKEHEEMLNNLEVGDK
                     IVTIGGIKGKIIKLKDNNIRLRISSDVDIDVVQKSIARLDTSEEEAEEPINDEKMLD"
     gene            complement(1457..2563)
                     /gene="tgt"
                     /locus_tag="VKN64_01155"
     CDS             complement(1457..2563)
                     /gene="tgt"
                     /locus_tag="VKN64_01155"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMA58868.1"
                     /translation="MSLNFDLLKKDSDSDARLGKIDTPHGEINTPIFMPVGTQATVKA
                     MSPEELKEIKAEIILGNTYHLYLRPGHELIEKAGGLHQFMNWDKPILTDSGGFQVFSL
                     ADLREIKEEGVYFQSHIDGSRQFISPEKSIEIQNALGSDIAMAFDECPPEPSNYNYVK
                     NSLERTVRWAERSKAAMNNDKQSLFGIIQGGIFKDLREESVKKMTDIGFPGYAIGGLS
                     VGEAKEDMLDTLEFTVPLMPDDKPRYLMGVGTPEDLIEGVKRGIDMFDCVMPTRIARH
                     GSIYTSQGRITIRNATYKEDFTAPDPNCDCYVCQNYTRAYIRHLLKRKEILGVRLTTY
                     HNLYFFIDLMNKIKKAVDKGNFLSFREKFYQNYK"
     gene            complement(2579..3601)
                     /gene="queA"
                     /locus_tag="VKN64_01160"
     CDS             complement(2579..3601)
                     /gene="queA"
                     /locus_tag="VKN64_01160"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMA58869.1"
                     /translation="MRVDDFNYELPERLIAQKPIENRDESRLMLLNKETGEIDEKIFY
                     EIVDLLNKEDLLILNNSKVIPARLYGKKVPTGTDIEVLLLTRLDDKRWEVLVKPGKRV
                     KKGVEISFGNGQLTGKAVEYTEFGGRVMEFDYEGDFEEILDKLGNMPLPPYIKEDLDE
                     PERYQTVYAKKRGSVAAPTAGLHFTPELLDEIEEKGVQVEYITLHVGLGTFRPVRTDE
                     VEEHDMHSEYIEVSEEVVNKIKKTKKGPGRVISVGTTVTRALETAAEDGELEEFKGWT
                     DIFIYPGFEFKVIDALITNFHLPKSTLLMMISALAGKEKIFKAYEKAVKEEYRFYSLG
                     DAMFIY"
     gene            complement(3617..4648)
                     /gene="ruvB"
                     /locus_tag="VKN64_01165"
     CDS             complement(3617..4648)
                     /gene="ruvB"
                     /locus_tag="VKN64_01165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMA58870.1"
                     /translation="MEDSDRFVSPTRKFEEDQFDKNLRPEKLKYYIGQQKTKEKLDIF
                     IEAAKKRGDALDHVLLYGPPGLGKTTLATIIANELNVNIHKTSGPAIERPGDLASILT
                     NLQDNDVLFIDEIHRLNMMVEEVLYPALEDFSLDIIIGKGPSARSVRLDLNPFTMVGA
                     TTRAGLISSPLRDRFGVINRLEFYEIDELTKIVKRSARILDIEIHDDGAHEIAKRSRG
                     TPRISNRLLKRVRDYAEVKAEGIISEEVVDQALQLLEIDEKGLDSIDHKLLEVIIKKF
                     DGGPVGLNTLAAAISEETETIEDVYEPYLLQMGFLERTPRGRVATAAAYRHLGINKNK
                     QDNLFDSEE"
     gene            complement(4649..5239)
                     /gene="ruvA"
                     /locus_tag="VKN64_01170"
     CDS             complement(4649..5239)
                     /gene="ruvA"
                     /locus_tag="VKN64_01170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMA58871.1"
                     /translation="MIGHLKGKLIYSNESSIILDVNGVGYQIEVPTLYTKKDLNKNLE
                     LFIHTYIREDALDLYGFETAEEKKLFKTLLSVSRVGPSAAMNILSSLAYERFINAILS
                     ENVAVLKEVSGIGPKTAQRLILELKSKIEDMLPELQIEKGKSMKKYDEDLYEALTQLG
                     YSTQEIDKAVNNLDLDTEMKLEDKIKNVLSYLGREH"
     gene            complement(5236..5733)
                     /gene="ruvC"
                     /locus_tag="VKN64_01175"
     CDS             complement(5236..5733)
                     /gene="ruvC"
                     /locus_tag="VKN64_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMA58872.1"
                     /translation="MIILGIDPGLATTGYGVIKKKGNSFELIAYDTIKTSPDQTDVDR
                     LSNLYEELLLLIDKYEPEHIAVEELFFNKNVKTAIRVGQARGVILLAGSQKGIEVAEY
                     TPLQVKQAVVGYGRASKNQVQQMVKALLNLDNIPKPDDAADALAISICHGHSYASKEK
                     WGDLL"
     gene            complement(5825..6586)
                     /locus_tag="VKN64_01180"
     CDS             complement(5825..6586)
                     /locus_tag="VKN64_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA58873.1"
                     /translation="MAGHSKWANIKHRKQKQDKKKGKLFSKLSRKIAVAAREGGGDPE
                     MNADLAFAIEKAKDNDMPKENIERAIKRGTGELEGVDYKEFTYEGYGPGGVALYFDIM
                     SDNRNRTASELRHILSEHGGNLGEEGCVAWMFDRKGQILIDLNSTDLDQEEVMMEAIE
                     AGAEDIETEDNLVTVYTEVNKFQDVKKELEKKGFELVESDLAMVPNNTIELEKSNAKK
                     MLNLMEELEDHDDIQEVYSNFDIPADVMEQIENEE"
     gene            complement(6688..7025)
                     /gene="rnpB"
                     /locus_tag="VKN64_01185"
     ncRNA           complement(6688..7025)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VKN64_01185"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7203..7742)
                     /locus_tag="VKN64_01190"
     CDS             complement(7203..7742)
                     /locus_tag="VKN64_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA58874.1"
                     /translation="MWILIFGIGLLISLIWGRFYCGWICPINTVIKIKSWFYKKMGID
                     TLQTPTVLKKGWLRWILLFIMIVSMIIMRRNNIQLNIILYILGIAFLISLLFEEELWH
                     KYLCPYGALLNITNKANNKNLVINESRCKSCGLCAENCPNNIITINKEEQAIGNNECL
                     YCFECQDVCEFEAISYSKN"
     gene            complement(7923..9149)
                     /locus_tag="VKN64_01195"
     CDS             complement(7923..9149)
                     /locus_tag="VKN64_01195"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HMA58875.1"
                     /translation="MKLLKNKCLITILSFLLILLVSLLTQVKAAFVSNSLTGRYVYDL
                     DQGDTYNSKLIYELDYENRIKTDYIFGKIIANPIFEYNLENKIEDINLNEAYLDLYFN
                     EIDLRIGKQNMTWGKSDGLVVTNIVNPRDYTVHPVVEYEDQFQSINAIKANLYPKNDT
                     LEIVWIPYFKSAKLNREVLLENLPQNFRKDSSEKEVEEKFANSELFLRYSSLGQKYDY
                     EIMAGYSWDDQPTLHKDFQKKAVIPQHHRLYTFGGSISTMRGPVVIRGEGAYISGKYF
                     NFANPLIHLQDYPEGVIEKDEIKWLIGFDYNYEDYLISLQFMQEKILDYQSDIVQDED
                     QNTITFLIKRDFLRSKLNTEINFHYNVNKEVLMSKPSISYDYTDSIKFKLGANLNLAG
                     DTFRTDVLYVQTEYLF"
     gene            complement(9178..>9702)
                     /locus_tag="VKN64_01200"
     CDS             complement(9178..>9702)
                     /locus_tag="VKN64_01200"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HMA58876.1"
                     /translation="DVRGTSFMNWTYQDNREDSQWIYLPALGKIKRISAENKSDYFMG
                     SDFTYDDLGERNIEEDTHTLLKSEELKGAECYVVENIPKEEDYMYFKTITWVSKENLI
                     GLKKEFYDPAGKLLKVLTVKDYQVINGIVTITHSEMENVQKEHKTVLKLDEVSYNTEI
                     PEDRFTERSMKIGI"
BASE COUNT         2910 a         2000 c         1279 g         3513 t
ORIGIN      
        1 tcataatcag cagttgcatt ttttacaaat tcacctgtca tcaatacttg accggctgga
       61 tttctgaagg tcaagaccgc agttctaccg attgtttcaa ttgcttcatt tggattatca
      121 acagcaggga gctctacgat tattctgtca tctccttctc tttgaataac cggttctgtc
      181 aatcctaatt gattaacacg cctttcaata acctgctgaa ttccagacat tacttcattt
      241 gttatatcac gttcttctgt aggttgagcc tgtaacacga tatgtgagcc accttgcagg
      301 tcaagcccaa ggtttatact attgttaaaa tataaaaata ccgctaaggc aactatggct
      361 atgataaaag caatttttat tagtcttttc tttttatatc tcataccact tcttcctccc
      421 gtttctcaac aattataata tttataaatt ttcgtattct acctgatcta ttatacgcat
      481 ctcagtaata ggccaataga cccaaaaagc taaaccagat attgtttcat atggtacata
      541 tccaacataa cctggatatc tactgtctgc actgttgttg cgattgtcac ccattacaaa
      601 taccgatttt tcaggaactt gataaggacc aaaattccct ttcatatctt ccttaatata
      661 atcttcttca agtggttgac cgttaacata agtaacacca tcctttatgt agacagtatc
      721 acccggtaaa cctattacac gcttaatgta ctttttgtta ggagcaccgc ggggagtaaa
      781 tacaacgact tctcctcttt gagggtccct aaaacggtaa ataaatttat tgacaaataa
      841 tctctctcca tcatggagat ttggctccat agatttaccg tcgacaacaa aggattgcgc
      901 aacaaaagta ataattaaaa atgctaaaat acctgcaata acaacagatt gcaggatctc
      961 ttttacatca ccgctatcca attttagtca ctcccagtat ctaactttta ttttataact
     1021 tgattaaatt ttttttaact tctcttaatt attaatcaag cattttttcg tcatttatag
     1081 gctcttctgc ttcttcttca gaagtatcta aacgagcaat agacttttgg acaacatcta
     1141 tatcaacatc agatgaaatt cttaatctaa tattgttatc tttaagttta attattttac
     1201 ctttgattcc acctatagtt actattttat ctcctacttc caaattattt aacatttctt
     1261 cgtgttcttt ttgctgcttt ctctggggtc tgattaagaa aaaccaaaac actccaaata
     1321 ttaagaccca tggtaatatt gcgtaaacat attgcatttt ttataacctc cttatttttt
     1381 tactctctaa taatttcaat accagattaa attatccttc aaatttttta aattaaaatg
     1441 attatctggc attaatttat ttataatttt gataaaattt ttctctaaaa gataagaaat
     1501 tacctttatc tactgctttt ttgatcttat tcatcaaatc aataaaaaag taaagattat
     1561 ggtaggtagt taatcttaca ccaagaattt cttttctttt taagagatgt ctaatataag
     1621 ctctcgtata attttgacat acataacaat cacagttggg atcaggagca gtaaaatctt
     1681 ctttatatgt tgcatttcta attgttatcc taccttggga tgtataaatc gaaccatgtc
     1741 ttgctattct ggtaggcatc acacaatcaa acatatcaat acctcttttt actccctcaa
     1801 ttaaatcctc cggggttccc actcccatta aatagcgtgg tttatcgtcc ggcattaagg
     1861 gaactgtaaa ttcaagagta tccaacatat cctcttttgc ctctcctacg cttaaaccac
     1921 cgattgcata accggggaaa ccaatatctg tcatcttttt gacactttct tctcttaaat
     1981 ctttaaatat tcctccctga ataataccaa atagactctg cttatcatta ttcatggcgg
     2041 cttttgaacg ttcagcccag cgtacagtgc gctcaagaga attttttaca tagttataat
     2101 tggaaggttc aggtggacat tcatcaaaag ccatcgctat gtcagaacca agtgcattct
     2161 gaatttcaat agacttttct ggagaaatga actgcctcga gccatctatg tgagactgaa
     2221 agtatactcc ttcttctttt atttctctta aatcagctaa actaaatact tgaaaaccgc
     2281 cactatcggt taaaatcggc ttatcccaat tcataaattg atgtaagccg ccggcctttt
     2341 cgatcaattc atgacccggt cttaaatata gatggtaagt attacccaaa attatttcag
     2401 ccttaatttc ttttaattct tccgggctca tggcctttac agtagcctgt gtaccgaccg
     2461 gcataaaaat gggggtatta atttctccat gtggagtatc tatcttcccc aacctggcat
     2521 ctgaatcact atcttttttt aacaagtcaa aattcaaact cattaattta cttcctttct
     2581 agtatataaa cattgcatct cctaaactgt aaaacctgta ttcttcttta actgcttttt
     2641 cataagcctt aaaaattttt tccttaccag ccaaagcaga tatcatcatt aataaagttg
     2701 atttgggaag atgaaaatta gtaattaaag catctatgac tttaaattca aaacccggat
     2761 agataaatat atctgtccag cccttaaatt cttctaattc accgtcttca gcagctgttt
     2821 ctaaagcacg agtaacagtg gttcccactg aaattactct acccggccct ttttttgttt
     2881 ttttaatttt gttgacaacc tcttcactga cttcgatgta ttcagaatgc atatcatgtt
     2941 cttcaacttc atccgtccgt accggacgga aagtacccaa tcccacatgt aaggttatat
     3001 attcaacctg aactcctttt tcttcgattt catccaataa ttctggtgta aaatggagtc
     3061 cagccgttgg agctgcaact gagcctcttt ttttagcata aacagtctga tatctttctg
     3121 gttcatcaag gtcttctttg atataaggtg gtaatggcat attacctaac ttatctaaaa
     3181 tttcctcaaa atcaccttca taatcaaact ccatgactct accgccaaat tctgtatatt
     3241 caacagcttt accggttaat tgaccattgc caaagcttat ttcaacacct tttttaaccc
     3301 ttttgccggg tttaactaaa acttcccagc gtttatcatc taacctggtt aaaagcagaa
     3361 cttcaatatc ggtgcctgtc ggaacttttt taccatataa tctggccggg ataactttac
     3421 tattatttaa aatcaagaga tcttctttat taagtaaatc tactatttca taaaaaatct
     3481 tctcatcgat ttcacctgtt tctttattta ataacatcag acgagattcg tctctgtttt
     3541 caatcggttt ttgagcgatt aatctttcgg gtaattcata attaaaatca tcgactcgca
     3601 tagttttcct cctgaatcat tcttcactat caaatagatt atcctgttta tttttattaa
     3661 tacctaaatg tctataggca gccgcagtgg ctactcttcc ccgtggggtc ctttctaaaa
     3721 aacccatctg cagtaagtac ggttcataaa catcttcgat tgtctctgtt tcttcactaa
     3781 ttgcagctgc gagagtattt aaaccgaccg gtccgccatc aaacttttta attattactt
     3841 ctaaaagttt atgatctata ctgtctaaac ctttttcgtc aatttctaat aattgaagag
     3901 cttgatccac aacctcttct gaaattattc cttccgcctt tacttcagca taatctctta
     3961 ctctttttaa taacctatta cttattcggg gcgtgccgcg tgacctcttg gcaatttcat
     4021 gggccccatc gtcatgaatt tctatatcta atattctggc agatctttta acaatcttgg
     4081 tcaactcatc gatttcataa aattccagcc tgttaattac tccgaatcta tctcttaacg
     4141 gagaacttat taagcctgcc cgggtagtag cgcctaccat tgtaaagggg tttaaatcta
     4201 gacgcacaga acgagcacta ggccccttac caataatgat atccaacgaa aaatcctcta
     4261 aggcaggata taatacttct tcaaccatca tattaagccg atggatctca tctataaata
     4321 atacatcatt atcctgaaga tttgttaaaa tcgaggctaa atctcccggt ctttctatgg
     4381 caggacccga agtcttatga atattaacat ttaattcatt agcaattata gtggccaagg
     4441 tagtcttgcc taaaccgggt gggccatata aaagcacatg atctaaggcg tccccgcgtt
     4501 tttttgcagc ttcaataaat atatcaagct tttctttggt tttttgttgg ccaatataat
     4561 attttaactt ttccgggcgt aaattcttat caaattggtc ctcttcaaat ttcctggtcg
     4621 gggatacaaa tctatcagaa tcttccattt agtgttccct ccccagataa cttaatacgt
     4681 ttttaatttt atcttcaagt ttcatctctg tatccaaatc aagattattg actgctttgt
     4741 ctatttcttg ggtactgtaa cccaactgag ttaatgcttc ataaaggtcc tcatcatatt
     4801 ttttcataga tttacccttt tctatctgca gttctggtag catgtcttca atcttactct
     4861 ttagttctaa aattaaccgt tgggcagttt taggacctat accagatact tcttttaata
     4921 cagctacatt ttcggaaaga attgcattaa taaaccgttc ataagcaagt gatgatagga
     4981 tattcatcgc tgctgatggg cctactctag atactgataa tagggtttta aataatttct
     5041 tttcttcagc agtctcaaaa ccgtataaat ctagagcatc ttccctaata taagtatgta
     5101 taaaaagttc taagttttta ttgagatctt tttttgtata taaggtggga acttctattt
     5161 gataacccac tccatttaca tctaaaataa tgctgctttc attactatat attaatttac
     5221 cttttagatg tcctatcata aaagatctcc ccatttttct ttactggcat agctatggcc
     5281 atgacaaatt gaaattgcca gggcatctgc cgcatcatct ggttttggta tattatccaa
     5341 attaagtaag gctttcacca tctgctgtac ctggttttta gaagctctcc cataacctac
     5401 tacagcctgt ttaacctgta aaggtgtata ttcagcaact tctatcccct tttgcgaacc
     5461 agctagtaat attacacctc tagcctgacc aactctaata gcagttttga catttttatt
     5521 aaaaaataat tcttctaccg ctatatgttc aggctcatat ttatctatta acaagagcag
     5581 ttcttcgtat aaatttgaaa gtctatctac atctgtttgg tcggggcttg tttttattgt
     5641 atcataagct atcaattcaa aagaatttcc cttctttttt ataacaccat atccagttgt
     5701 agctaaaccg ggatcaatcc ctaaaataat caaatgacca cctcatcaaa caaaaaaatt
     5761 atattaataa attaataaat gacactgaaa attcagtgcc attcaatgag taaattaatt
     5821 gtatttattc ctcattttca atctgttcca tgacatctgc aggtatatca aagttggaat
     5881 acacttcttg tatatcatca tgatcttcta attcttccat aagatttaac atctttttgg
     5941 cattagattt ttctaattca attgtattat taggaaccat agccaaatca gattcaacca
     6001 gttcaaaacc ttttttctca agttcttttt taacatcctg aaatttattt acttctgtat
     6061 aaactgttac taaattgtct tcagtttcaa tatcttcagc gccagcttca attgcttcca
     6121 tcattacctc ttcttgatct aaatcagtgg aattaaggtc aattaaaatt tggccctttc
     6181 tatcaaacat ccaggctaca cagccttctt cacctaaatt accaccatgt tcagataaaa
     6241 tatgtcttaa ctctgaagct gttctatttc tattatcact cattatatca aaataaagag
     6301 ctacaccacc cggaccataa ccttcatagg taaattcttt ataatcgaca ccttctaact
     6361 cacctgtgcc cctttttata gctctttcaa tattttcctt tggcatatca ttatcttttg
     6421 ccttctcgat ggcaaaagct aaatcagcgt tcatttcagg atctccgcca ccttcgcggg
     6481 cagctactgc aatttttctc gataatttag aaaatagttt accttttttc ttatcctgtt
     6541 tctgctttct atgtttaata ttagcccact tagaatgtcc ggccatatta ttacctccta
     6601 aatctttgtt ctgctgttta ttttaacata atttagcctt attttcaatt tagcagacaa
     6661 aaaaataaaa catatgtttg aacgttgaat aagagcagca cggtaagccg ggttctgttt
     6721 tagataatca tctatctagg acagcgatta ctcactgcct caagcgtcca acccggagga
     6781 aaagcgggtc actttacttc ctccctattt ggacttactt cggatggggt ttaccaaact
     6841 atccagtcac ctggatattg gtgagctctt acctcacctt ttcaccctta ccataaaaat
     6901 ggcggtgtat tttctgtggc actttcctgg gagttacctc cactggggat tacccagcat
     6961 cctaccctat gaagcccgga ctttcctcgg tagagaaagt ctaccgcgat tatctgtgat
     7021 gctcttattc aactatggca ttaacagtat agcaatcatt ttatgtttag tcaagccctg
     7081 ggattttaat taaaggtcaa tacttttaaa tttaaaaagt agtaacctca caatctttat
     7141 ttgattgtga ggtaccaaat ttttaatcct atgtttaatt ttaatttctt ttaaaactta
     7201 ttttaatttt tactatatga aattgcctcg aactcacata catcctgaca ttcaaaacaa
     7261 tataaacatt cattatttcc aatagcctgt tcctctttat tgattgttat gatattatta
     7321 ggacaatttt cagcacacaa tccacaactt ttgcatctgc tttcattaat cactaaattt
     7381 ttgttgtttg ctttatttgt tatatttaat aaagcgccgt agggacatag atatttatgc
     7441 cagagttctt cctcgaaaag caatgatatt aagaaagcta tacctaagat atataatatt
     7501 atattaagtt gtatattatt ccttctcatt ataatcatgg acactatcat tataaacaat
     7561 aatatccatc ttaaccaacc ttttttaagt actgtcgggg tttgcaaggt atctattccc
     7621 atttttttgt aaaaccaact tttaattttt ataacggtat tgatgggaca gatccaacca
     7681 cagtaaaatc tcccccatat taaggaaatt aatagtccta tcccaaaaat taatatccac
     7741 aattgggctc ttcccctcac aaccagcata aaaaatatta ttaaaaatat aatctgaact
     7801 atatttctca ttaaagactt atcctcaaag tatttaattt ctaatcccct ccttctttca
     7861 aactaggtta aaacaactac aacataggca actaatttaa agttgcctat gtcagcctct
     7921 ttttagaata aatattctgt ttgaacataa agtacatcag ttctgaatgt atctccggct
     7981 aaatttaaat tagctcccaa tttaaatttt attgaatctg tgtaatcata acttattgat
     8041 ggtttagaca ttaatacttc cttgttaaca ttataatgaa agtttatctc tgtatttaat
     8101 ttagacctta aaaaatccct cttgataagg aaagttatcg tattttgatc ttcatcttga
     8161 actatatctg actgatagtc taatattttt tcctgcatga actgaagact aattaaataa
     8221 tcttcatagt tataatcaaa accgattaac catttaatct catctttttc gattactccc
     8281 tccggataat cctgtaagtg aattaagggg ttagcaaaat taaaatattt tccactaata
     8341 taagctccct cacccctaat gaccaccggt cccctcatag tacttatact accaccgaaa
     8401 gtatacaaac ggtggtgttg agggataact gctttttttt ggaagtcctt atgaagagtc
     8461 ggttggtcat cccaactgta accggccatg atttcataat catatttttg tccgagggaa
     8521 gaatatcgca gaaaaagttc actgtttgca aatttttctt ctacttcttt ttcactgctg
     8581 tcttttctaa aattctgagg caaattttct aataagacct ctctatttaa tttagctgat
     8641 ttaaagtatg gaatccatac tatctctaga gtatcatttt ttggatataa attagctttt
     8701 atagcattta tactttgaaa ttggtcttca tattcgacaa caggatgaac agtatagtct
     8761 ctaggattca caatattggt tactactaat ccatcacttt taccccaggt catattttgt
     8821 tttccaatcc ttagatcaat ttcattgaaa tataaatcta aataagcttc attcaagtta
     8881 atatcttcaa ttttattctc taaattgtac tcaaatatag gattggctat aattttccca
     8941 aagatataat cagtctttat cctattttca tagtctaatt catatatcaa tttagaatta
     9001 taagtatcac cctgatccag atcatacacg tatctaccgg ttaaagagtt tgatacaaag
     9061 gctgccttaa cctgcgtaag caatgaaact aacaaaatta gcagaaatga taaaatagta
     9121 ataagacatt tattttttaa caatttcatc gatctcaccc ctaataagct catatactta
     9181 aatccctatt ttcatgcttc gttctgtaaa tctatcttca ggtatttcgg tattgtaact
     9241 tacttcatca agttttaaaa cggttttatg ctccttctgt acattttcca tctcactatg
     9301 agtaatagta acgatgccgt taataacttg atagtcttta acggttaata cctttaataa
     9361 tttaccagct ggatcataaa attctttttt aagaccaatt aagttctctt tactaaccca
     9421 ggtaatagtt ttaaaataca tataatcttc ttcttttgga atattctcta ccacataaca
     9481 ttctgctccc ttgagttctt cactctttaa taaagtatga gtatcttctt ctatatttct
     9541 ttctcctaaa tcatcatagg taaaatcgga ccccataaag taatcgcttt tattctccgc
     9601 tgaaattctt tttattttac ctaaagcagg taaataaatc cactggctgt cttctctatt
     9661 atcttgataa gtccaattca taaaggaagt ccctcttaca tc
//
LOCUS       DATGCJ010000026         7632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_360747, whole genome shotgun sequence.
ACCESSION   DATGCJ010000026 DATGCJ010000000
VERSION     DATGCJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360747"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..3646
                     /gene="rpoB"
                     /locus_tag="VKN64_01205"
     CDS             401..3646
                     /gene="rpoB"
                     /locus_tag="VKN64_01205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMA58877.1"
                     /translation="MSTVRERHSFAKIDDAMDLPNLIHTQLNSYQWFLEQGLHEVFEE
                     ISPIEDFSENLVLEFVGYHLEEAKYTVQECRDRDDTYSAPLQVKVRLINKETGEVKEQ
                     EVFMGDFPLMTDKGTFIINGAERVVVNQLVRSSGVYFDDERTKDGRRLINGSIIPNRG
                     AWIEFEYDKKRIISVRVDRKRKMPSTILFRALGYGSDEELIDLLGDSKVIMDTLDRDS
                     TDSQDEALIDLYKKLRPGEPPTVDSAKSLIESLFFDPKRYDLAAVGRYKINKKLDLDI
                     DEDKKYLDEKDIIETVRYMVRLVHEEEDAYIDDIDHLGNRRLKTVGELLQNQFRIGLS
                     RMERVVRERMTIQDIDVVTPQALMNTRPVVAAIQEFFGSSQLSQFMDQTNPMSELTHK
                     RRLSALGPGGLSRERAGFDVRDVHHSHYGRICPIETPEGPNIGLIGSMGTYAKTNEFG
                     FLMTPYRRIVDGKTTGDIDYLTADEEDQNIVAQANTKLNEDGEIVSDEVVARHRGDIL
                     EVPPEKVDYMDVSTKQLVGVSAAMIPFLENDDANRALMGANMQRQAVPLLKTDAPIVA
                     TGIEHKAAKDSGVVVIAKNGGTVRKVTSSKIVIENNDGNIDKYNLMKFKRSNQGSCMN
                     QTPIVTEGQEIEENDIIADGPSTDQGEMALGRNSLIAFMPWEGYNYEDAILISEKLVK
                     EDAFTSIHIEEHEAEARDTKLGPEEITRDIPNVSESSLKDLDDRGIIRVGAEVKEGDI
                     LVGKVTPKGETELSAEERLLRAIFGEKAREVRDTSLKVPHGEEGIVVDVKVFSRDQGD
                     ELKPGVNKLVRVYIANKRKIAVGDKMAGRHGNKGVISRILPEEDMPFKENGEPVEIVL
                     NPLGVPSRMNIGQVLETHLGMAAKALGLYVETPVFNGALEEEVEEYLEKADLNRNGKM
                     TLYDGRTGESFDHDITVGYMYILKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQ
                     RFGEMEVWALEAYGAAYSLQEMLTVKSDDVIGRVKTYESIVKGEEVPPPGVPESFKVL
                     IKEMQSLGLDARVFVEEDNEMELEEEGTEVSETAKKLGLDTDLQLSRPDEG"
     gene            3678..7154
                     /gene="rpoC"
                     /locus_tag="VKN64_01210"
     CDS             3678..7154
                     /gene="rpoC"
                     /locus_tag="VKN64_01210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMA58878.1"
                     /translation="MANVNNFDSMRIGVASPEQIREWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDYECHCGKYKRVRYKGVVCDRCGVEVTRSKVRRERMGHIELAAPCT
                     HIWFFKGIPSRLGLIMDMSPRALEKVIYFVSYIVIDPGDTPLSERQLLSEQEYREAKS
                     KYGNEFDARMGAEAIKELLSKIDVQKDVDELKKEVKSTSGQRRRRAIRRLEVMDGLLD
                     SGLEPEWLVMDVMPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PEIIIRNEKRMLQESVDALIDNGRRGRPVTGAGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELRMDQCGLPKKMALELFKPFVMKGLVDKEYAHNIKNAKKMVEK
                     VDDRVWGILEEVIKGHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKLHPLVCPPYNADF
                     DGDQMAVHLPLSEEAKTEARTLMLSTTNLLAPADGNPITVPTQDMVLGIYYLTTIKEG
                     IKGEGKIFSRPAEAIKAYDTDKIYLQAPIKARIDGEIIDTSVGRIIFNEALPEDMEFI
                     NERIDKGGLGDLINDLYDEFGEEKTVKSLDNIKELGYDFATRSGISIAVTDADIPEEK
                     PKILKEADEEVDEIEEHYNRGAITEDERYHRVIDIWNQAKDDVTDALLDHLDEDNNIY
                     IMATSGARGNTSQISQLAGMRGLMADPSGRIIDLPIRSNFREGLNVLEFFLSTHGARK
                     GLADTALRTADSGYLTRRLVDVSQDVIVKAEDCETEKGLYVEAIGGHGSQAVESLRER
                     IAGRYAAEDLKDPASGEIIVERNEMIKKDHLDKIEKTDIDSVKIRSVITCESEHGVCT
                     KCYGRNLATTNKVNIGESIGIVAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVEE
                     LFEGRTPKGHAIMTEKEGKVKITQKRNSKRIVVTSDDGKKKTYKIPYGSKLIVQEGEY
                     VEPGDKLTEGPLDPSVLLRVKGEAAVQNYLLKEVQNVYRSQGVEINDKHIEVIIRQMM
                     KRVKIKESGDTDLLTGSLVTKHEFKETNQKVKANGGKPAKAIPQILGITKASLATESF
                     LSAASFQETTRVLTEAALEGKIDRLRGLKENVIIGQLIPAGTGMRKYRDGEELGENVT
                     DVTQVLEEVEEE"
     gene            7351..>7632
                     /gene="rpsL"
                     /locus_tag="VKN64_01215"
     CDS             7351..>7632
                     /gene="rpsL"
                     /locus_tag="VKN64_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMA58879.1"
                     /translation="MPTINQLIRKGRNKQKKKKNAPALEGSPQKRGVCTRVYTATPKK
                     PNSALRKVARVRLTNGKEVTCYIPGEGHNLQEHSVVLVRGGRVKDLPGVR"
BASE COUNT         2597 a         1208 c         1749 g         2078 t
ORIGIN      
        1 aacaagtaga aaagattttt attgaaggta atagtgatat aactgaggta attaatactg
       61 tcaaagatag tttagatgaa ttaggtagta atattattaa aaatttttta gaggaatgcg
      121 ataaaaggat taaagagtca aaaattactt gacaagatat gggttctgtg ataacattac
      181 ttattgtcga taacttttca tataatggca taaaatatgc atacatttta gcttattttt
      241 ccttttttat tggattttta cggacttatt ttattatatt atttaataaa aatacattat
      301 aataacattt atttcattat tattaccaag catttaagca gggtatctct ttgcttaaat
      361 gttatattta taaatataag tattccttgg gggtgcaatg atgtcaacag tgagagagag
      421 acacagcttt gctaaaattg acgatgcaat ggatttaccc aatttaatcc acacacagtt
      481 aaattcatac cagtggtttt tagagcaggg cttacatgaa gtatttgaag aaatctcacc
      541 aattgaagat ttttcggaaa atttagttct agaatttgtt ggttaccatt tagaagaagc
      601 taagtatact gtacaggaat gtagggacag agatgataca tattcagctc ccctgcaagt
      661 aaaagttcga ttgattaata aagaaaccgg tgaagtaaaa gaacaggaag tatttatggg
      721 cgactttccc ctcatgactg ataaaggaac ctttattatt aatggggcgg agcgagttgt
      781 tgttaatcag ttagttcgtt cctctggtgt atatttcgat gatgaacgta ccaaagatgg
      841 acgtagattg ataaatggaa gtattatccc caatagaggg gcttggatcg agtttgaata
      901 tgataaaaag agaataatat ccgtgcgtgt tgataggaaa agaaagatgc cctctaccat
      961 attatttaga gccttaggtt atggtagtga tgaagaactt attgatttat tgggtgattc
     1021 aaaagtaatt atggatactt tagatcgtga ttcaaccgat tcgcaggatg aagctctaat
     1081 tgatttatat aagaaattaa gaccaggtga acccccgact gtagatagtg caaaaagttt
     1141 aatcgaatca ttattttttg atcccaagag atatgatttg gccgcagttg gtaggtataa
     1201 gataaacaaa aagcttgatt tagatattga tgaagataaa aaatatttag atgaaaaaga
     1261 tattattgag accgtacgtt atatggttag attggtacat gaagaagaag atgcctatat
     1321 agatgatatt gaccatctgg gtaatagaag actgaaaaca gttggtgaac tactgcaaaa
     1381 ccagttcaga attggtcttt cccgcatgga aagagtagta cgagaaagaa tgacaattca
     1441 ggatattgat gttgtaactc cccaggcttt aatgaatacc aggcctgttg tggctgcaat
     1501 tcaggaattc tttggaagca gtcagctttc acagtttatg gaccaaacca atcctatgtc
     1561 tgaacttacc cataaaagga gattaagtgc tttaggaccc ggtggtttaa gtagagaaag
     1621 agccggattt gacgttagag atgttcacca ttctcactat ggtagaattt gtcctattga
     1681 aactcccgag ggacctaata tcggtcttat tggttcaatg ggtacttatg ctaagacaaa
     1741 tgaatttggc tttttaatga ccccttatcg gagaattgtc gatggtaaaa caaccggaga
     1801 tattgattat ttaactgctg atgaggaaga tcaaaatata gttgctcagg ctaataccaa
     1861 gttaaatgaa gacggtgaaa ttgtaagtga tgaagttgtg gcccgtcacc ggggtgatat
     1921 tttagaggta ccgcctgaga aagtagacta catggatgtt tccaccaaac agctggtggg
     1981 tgtctctgct gctatgattc cattcttaga gaatgatgat gctaaccgtg ctttaatggg
     2041 tgctaacatg cagagacagg ctgtaccgtt actgaagacc gatgccccca tcgtggcaac
     2101 cggtattgaa cataaagctg ccaaagattc aggggttgtc gtgattgcta aaaacggtgg
     2161 tacagttcgt aaagtaacat caagtaagat tgtaattgaa aataatgatg gtaatataga
     2221 taaatataat ttaatgaagt tcaaaagatc aaaccagggt tcctgtatga accaaacccc
     2281 tattgtaaca gaggggcagg aaatcgaaga aaacgatatt attgcagatg gtccttctac
     2341 cgatcaaggg gaaatggcct taggtagaaa ctcccttatc gcatttatgc cctgggaagg
     2401 atataattat gaggacgcta ttttaataag tgaaaaatta gtaaaagaag atgcatttac
     2461 ttctattcat atagaagaac atgaagcaga agctcgcgat actaaattag gacccgaaga
     2521 gatcaccaga gatattccca atgtaagtga aagttcctta aaagacctcg atgatcgagg
     2581 tattattaga gtgggagcgg aagtaaaaga gggcgatatc ctggtaggta aggttacacc
     2641 aaaaggtgaa actgaactat cagcggaaga aagattactg agagctattt ttggtgaaaa
     2701 agctagagaa gttagagata cctctttgaa agttccacac ggtgaagaag gtatcgtagt
     2761 tgatgttaaa gttttctcta gagatcaagg tgatgaacta aaacccggcg taaataaact
     2821 tgtaagagta tatattgcaa ataaaagaaa gattgctgtt ggagataaaa tggcaggtcg
     2881 ccatggtaac aaaggtgtaa tttccagaat tctgccggaa gaagacatgc cctttaagga
     2941 aaatggagaa ccggtagaaa tagtacttaa cccattaggt gtaccttctc gtatgaatat
     3001 cggacaggtc ttagaaacac accttggtat ggcagcaaaa gcactcggct tatacgtcga
     3061 gacacccgta tttaacgggg ccttagagga agaggtagaa gagtaccttg aaaaagccga
     3121 cctaaataga aatggtaaaa tgaccctata tgatggtaga acaggtgagt catttgacca
     3181 tgatataaca gtaggatata tgtatatcct caaattacac caccttgttg atgacaaaat
     3241 tcatgcccgc tcaaccggac cttattctct ggttacccag cagccgctcg gcggtaaagc
     3301 tcaattcggt gggcagagat tcggcgagat ggaagtttgg gcccttgaag cttatggggc
     3361 tgcctacagc ttacaggaaa tgctaacagt taaatccgat gatgtaatcg gaagagtaaa
     3421 aacctatgaa tcgatcgtca aaggtgaaga agttccacca ccgggtgtac ctgaatcctt
     3481 taaagttctg atcaaagaga tgcagagtct cggtttagat gcccgcgtat ttgtggaaga
     3541 agataatgaa atggaattag aagaagaagg tactgaagta agtgaaacag ctaaaaaact
     3601 gggtctagat actgatctac agttatcaag acctgatgaa ggataaaaat tttacaaaat
     3661 aggaggggga attacccttg gcaaacgtaa ataatttcga ttcaatgcgg atcggagttg
     3721 catctcctga gcagataaga gaatggtctc gcggtgaagt taaaaaacct gaaaccatta
     3781 actatagaac tcttaaaccg gaaaaagatg gattattttg tgagaaaata tttggaccga
     3841 ctaaagacta tgaatgtcat tgtggtaaat ataaaagggt aagatataag ggtgtagttt
     3901 gtgaccgctg tggtgtagaa gttacccgtt ccaaggtaag aagagaaaga atgggtcaca
     3961 tagaattggc tgccccctgt acacatatct ggtttttcaa aggaatccct agtagactgg
     4021 gactaattat ggatatgtca ccgagggcgt tagaaaaagt aatatacttt gtttcctata
     4081 tagtaatcga tcccggtgat accccactta gtgaaagaca gcttctatct gaacaggaat
     4141 accgtgaagc taaatctaag tatggtaatg aatttgatgc ccgtatggga gcagaagcga
     4201 tcaaagaact attaagtaaa atagatgtac aaaaagatgt tgatgaactt aaaaaagaag
     4261 ttaaatcaac ttctggtcag cgcagaagac gcgctatcag aagactggaa gtaatggacg
     4321 gtcttttaga ttcgggcctt gaaccagaat ggctggtcat ggatgtgatg cccgttattc
     4381 cacctgacct aagaccgatg gttcagttag atggaggtag atttgctact tctgatttaa
     4441 atgacttgta tcggagagtt attaatagaa ataatagatt aaaaagactg cttgacctgg
     4501 gcgctcctga aatcattata agaaatgaga aaaggatgct gcaggaatca gtcgatgctt
     4561 taattgataa cggtagaaga ggtaggccgg taaccggagc cggtaatagg ccgcttaaat
     4621 ctttaagtga tatgcttaaa ggtaaacagg gtcgtttccg ccaaaatcta ctcggtaaac
     4681 gtgtagatta ttccggtaga tctgttatcg ttgttggtcc tgaattaaga atggaccagt
     4741 gtggtctacc aaagaaaatg gccttagaat tattcaaacc ttttgtaatg aaaggtcttg
     4801 tagataaaga atatgcacat aatatcaaaa atgcaaagaa gatggtagaa aaggttgatg
     4861 atagggtctg gggtatacta gaagaagtaa ttaaagggca tccggtctta cttaaccggg
     4921 caccgaccct gcacagatta ggtatccagg cttttgaacc tgttttagtg gaaggtaagg
     4981 ctattaagtt acatccctta gtctgcccac cctataacgc tgactttgat ggggaccaga
     5041 tggctgtcca cttaccatta tcagaagaag ctaaaacaga agcaaggacc ttgatgttat
     5101 caaccacaaa ccttttagcc ccagctgatg gtaatccaat aacagtacct acccaggata
     5161 tggttttagg tatctattat ttaactacta tcaaagaagg tattaaggga gaaggaaaga
     5221 tattctcccg gccggcagaa gctattaaag cttatgatac agataagata tacctgcagg
     5281 cccccattaa agccagaatt gatggtgaaa taattgatac ttcagtggga aggatcatct
     5341 tcaatgaggc tttaccggaa gatatggaat ttattaatga aagaattgat aaaggtggtt
     5401 taggcgattt aattaatgac ctttatgatg aattcggaga agagaagacg gttaaatcac
     5461 ttgataatat aaaagaatta ggttatgatt tcgcaactcg ttctggtatc agtattgccg
     5521 taactgatgc tgatataccg gaagagaaac cgaaaatttt aaaagaagct gatgaggaag
     5581 tagacgaaat agaagagcac tacaatagag gtgcaattac agaagatgag agatatcacc
     5641 gtgtaataga tatctggaat caagctaaag acgatgttac tgatgcccta ttagaccatt
     5701 tagatgaaga taataatatc tatatcatgg ctacctccgg tgctcgtggt aatacttctc
     5761 agatttctca gttagctggt atgcgtggat tgatggcaga tccatccggt agaattattg
     5821 acctacctat taggtctaac tttagagaag ggcttaatgt gttagagttt ttcctctcta
     5881 cccacggtgc tagaaaaggt ctggccgata ctgctttaag aactgctgac tctggttatc
     5941 taaccaggcg gttagttgat gtttcccagg atgtgatagt aaaagcagag gactgtgaaa
     6001 cagaaaaagg actatatgtt gaagcaatcg gtggtcacgg atcgcaggct gttgaatctc
     6061 tgagagaaag aattgccggc cgctatgcag ctgaggatct taaagatccg gccagcggtg
     6121 agataattgt tgagagaaat gaaatgatca aaaaagatca tttagataaa attgaaaaaa
     6181 cagatattga tagtgtaaaa atccgttcgg tgattacctg tgaatctgaa catggagtat
     6241 gtactaaatg ttatggccgc aacctggcta ctactaataa agttaatatc ggggaatcaa
     6301 tcggtattgt agcagctcaa tcaatcggtg aacctggtac ccagttaaca atgcgtacct
     6361 tccacaccgg tggagttgcc ggcgatgata tcactcaggg tctaccgaga gtggaagagt
     6421 tatttgaagg acgtaccccg aaaggtcatg ccatcatgac tgaaaaagag ggtaaggtta
     6481 aaataactca gaaaagaaac tctaaacgga ttgttgttac cagtgatgac ggtaagaaga
     6541 agacatacaa gattccttac ggttctaaat tgatcgtaca ggaaggcgag tatgtagaac
     6601 ctggtgataa actaacggaa ggtcccttag atcccagtgt tttactcagg gtaaaaggtg
     6661 aagctgccgt tcagaactat cttttaaaag aagttcagaa tgtatataga tctcaaggtg
     6721 ttgaaattaa tgataaacac attgaggtaa tcatcagaca gatgatgaaa agagtaaaaa
     6781 tcaaagagtc gggcgatacc gacctattga ctggtagtct tgttacaaaa cacgagttta
     6841 aagaaaccaa tcaaaaggtt aaagcaaatg gcggcaaacc cgcaaaagct attcctcaaa
     6901 tcttaggaat tacaaaggct tccctggcta cagaaagctt tttatcagct gcttctttcc
     6961 aggagacaac aagagttcta actgaagctg ctctggaagg aaaaattgat cgcctaagag
     7021 gacttaaaga gaacgtaatt atcggtcaat taatcccggc tggaactgga atgagaaagt
     7081 acagagatgg ggaagaactc ggtgaaaatg tgaccgatgt tacccaggtt ctcgaggaag
     7141 ttgaagaaga gtaaatttaa tgaggactta ggtattgaca acacatatca aaggtgttaa
     7201 aatacactag tgtgtgcaga caaaaggtgt ttggccttta ataatttaat cattatagat
     7261 aaataaaaag tattcagcgc agaggacagc attcataagg atgctggttc tctgctgcat
     7321 tactgaaata accggaagga ggtgggtttt gtgccaacaa tcaatcaact tatccgtaaa
     7381 ggtcgtaata aacaaaagaa gaaaaagaat gctcctgcat tagagggatc acctcaaaaa
     7441 agaggggtat gtacaagagt ttatacagcg actcccaaaa aaccgaactc agctttaaga
     7501 aaagttgccc gtgttcgttt aacgaatgga aaagaggtga cctgttacat acctggagaa
     7561 ggacataact tacaggaaca ctctgttgtg ttagtaagag gtggccgtgt aaaagactta
     7621 ccaggtgtta ga
//
LOCUS       DATGCJ010000027         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_182155, whole genome shotgun sequence.
ACCESSION   DATGCJ010000027 DATGCJ010000000
VERSION     DATGCJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182155"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VKN64_01220"
     CDS             complement(<1..913)
                     /locus_tag="VKN64_01220"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMA58880.1"
                     /translation="MALKQKDFLGLYNVSGEDINQILTTTESMKEIFTRTVKKVPTLR
                     GKTIVNLFFEPSTRTKSSFDLAAKRLSADVMSISKSTSSVAKGESLLDTSKTMEVMGA
                     DVVVIRHSAPGAAEFLANNIEASVLNAGDGAHAHPTQGLLDLYTMKSYKGSFKDMKVL
                     IIGDISHSRVARSNIQGLKELGAEVRVTGPATLIPREIEKMGVKFYKDLDKALEGVDV
                     VNILRIQLERQESGLFPSIGEYRKFYGMNKKRLDSLKDDVLIMHPGPMNRGIEIESEV
                     ADSEQSVITEQVTNGVAVRMALLYLLAG"
     gene            complement(1319..1873)
                     /gene="pyrR"
                     /locus_tag="VKN64_01225"
     CDS             complement(1319..1873)
                     /gene="pyrR"
                     /locus_tag="VKN64_01225"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMA58881.1"
                     /translation="MEEKDMKVKKEIINKDDIRRALTRISHEIIEKNEGTEDLVLIGI
                     RSRGVPMAERIAQRIGEFEGVEIPTGILDITLYRDDLSQVSKQPIIHQTEISFDITDK
                     QVVLTDDVLYTGRTVRAALDALIDLGRPKSIQLAILVDRGHREFPIRPDYVGKNLPTS
                     QSEVVDVNFEEIDGKDNVLLLEKE"
     gene            complement(2115..>2807)
                     /locus_tag="VKN64_01230"
     CDS             complement(2115..>2807)
                     /locus_tag="VKN64_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HMA58882.1"
                     /translation="EEFSYLINSEYNLDKTMIILNGDGASWIQKTSYDYFDNLIVQLD
                     RFHIKKDITIHFGKQIAEDLTGVLAGGKKKLFLDTLESLIYDGDNGKIIEGRETLFKH
                     YKKYEGHLLDYRHRIKVKDNYPELHGMGAIESYIGKIISRRMKNQGMSWKRLGAEAMV
                     KILMLRHNEILETRLNDKSYKIKNPIKELKYRERVFKRNWNDWLQASRTVIYGPYSGH
                     NWVKGIKSIATI"
BASE COUNT          822 a          603 c          359 g         1023 t
ORIGIN      
        1 tcccggctaa taaataaagt aatgccattc taactgcaac tccatttgtt acctgttcag
       61 taataactga ttgctcacta tcagcaactt cactttcaat ttcaataccc ctgttcatag
      121 gaccgggatg cataattaaa acatcgtcct taagactatc aagtctcttt ttattcatcc
      181 cataaaactt tctatattct cctatcgaag ggaataatcc actttcctgt ctttccagtt
      241 ggatccttaa aatgtttact acgtctaccc cttctaaagc cttgtccaaa tctttataga
      301 actttacccc cattttttct atttccctcg ggattaaagt ggctggacca gtaaccctta
      361 cttcagcgcc cagttctttt agcccctgaa tatttgatct tgccacccga ctgtggctga
      421 tatctcctat aataagaact ttcatatctt taaagcttcc tttataagat ttcattgtat
      481 aaagatccag taaaccttgc gttgggtggg catgtgcccc atccccggca ttaaggacac
      541 ttgcttcaat gttgtttgct aaaaattcgg ctgccccggg agcactatgt cggatcacta
      601 ctacatctgc tcccattact tccattgtct tagaggtatc taaaagtgat tctcccttgg
      661 ctacactgct ggtagatttt gaaatactca ttacatctgc actcagtctc tttgcggcca
      721 gatcaaagga agatttagtt cttgtactcg gttcaaaaaa tagattaaca attgtctttc
      781 ccctaagggt aggaactttt tttactgttc ttgtgaaaat ttctttcatt gattcagtag
      841 ttgttaaaat ttgattaata tcttctcccg aaacattata taaccctaga aaatcttttt
      901 gttttaaagc cattttctac ctcctgtttc ccttttgaat tatgatttaa ttaatatttt
      961 aaacagttgt atttttcctc catctggaac atataccaac attatcattg gttttatcta
     1021 taaataactt ccaattattc agtaaatcta caccatatct tattaaaatg atttatccgt
     1081 ttggatctat atttgcaagt ttaagaaaac aaccccccct ttactagatt gcctattata
     1141 gaagtaaagc agatccttat catttcaact tccttttttg acataaaaaa agccttctca
     1201 tcagttcagt caaaactgac aagaaggtta atatcttcta tacctatagt aaaggtttaa
     1261 aagtcccttg ccagtctcgc tggactgaat taaagggtct tactgtttaa ttttaaagtt
     1321 attctttttc taaaagtaaa acattatctt ttccatctat ctcttcaaaa ttaacatcta
     1381 caacttcact ttgagaggta ggtaaatttt taccaacata atctggtctt atcggaaatt
     1441 ctctatgtcc tctatctact aaaatagcta attggataga tttgggtctt cctaaatcta
     1501 tcaaagcatc aagagcagct cttaccgttc tcccagtata taaaacatca tcggttaaaa
     1561 ctacctgctt atccgtaata tcaaaggata tttcagtttg atgaataatg ggctgtttag
     1621 agacttgtga taaatcatct cgatataagg taatatctaa aataccagtt ggaatctcta
     1681 caccctcaaa ctcacctatc ctttgggcta tcctttcagc cataggaaca cctctagagc
     1741 gaattccaat taacactaga tcttcagttc cctcattttt ttcaattatt tcatgagata
     1801 ttcttgttaa ggcccgccta atatcgtcct tattaataat ctctttttta actttcatat
     1861 ctttttcttc catattaact accccttctt tttatagata taacaataca aataaaaaaa
     1921 ccttctaccg gcaaggtaag aaggctaaat aaataatatt atttctctta ttagcctcac
     1981 cggactaatt taaagcgcta tttttatatt aactatctaa acagtatcat aacttcaatt
     2041 aagtgtcaag gttcaattaa cataagtttg cgtaaagaaa aggtgggaaa gataaagacc
     2101 tcaatatatt ttatttatat agtcgctatt gactttatgc ctttaaccca gttatggccg
     2161 ctgtaaggtc catatataac tgtcctactt gcttgcagcc aatcattcca gttcctttta
     2221 aagactcttt cccgatattt taattctttt ataggattct ttatcttgta agatttgtca
     2281 tttagtcttg tttctaatat ttcgttatgc ctcaacatta agatcttaac cattgcttcg
     2341 gctcccagcc ttttccaact cataccctga tttttcattc gcctagatat aatcttgcct
     2401 atataactct caatcgctcc cataccatgt aattcgggat aattatcctt aaccttaatc
     2461 cgatgtctgt aatccaatag atgaccttca tatttcttat agtgcttaaa gagggtctcc
     2521 ctgccctcta ttatctttcc attatcacca tcataaatca gtgattctaa agtatctaaa
     2581 aatagcttct ttttcccgcc tgccaatact cccgttaaat cttcggctat ctgtttaccg
     2641 aaatgtatag taatatcttt tttgatatga aaacgatcaa gttgaactat taaattgtca
     2701 aagtaatcat aacttgtctt ttgaatccag ctcgctccgt ctccatttag aataatcatt
     2761 gtcttatcta aattatactc cgaatttatt aaatagctga attcctc
//
LOCUS       DATGCJ010000028         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_182208, whole genome shotgun sequence.
ACCESSION   DATGCJ010000028 DATGCJ010000000
VERSION     DATGCJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182208"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..303)
                     /gene="infA"
                     /locus_tag="VKN64_01235"
     CDS             complement(79..303)
                     /gene="infA"
                     /locus_tag="VKN64_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMA58883.1"
                     /translation="MAKEEPIEVQGTVIEPLPNAKFKVELENGHEVLAHVSGKMRMNY
                     IRILPGDKVTVELSPYDLSRGRITYRHKAK"
     gene            complement(392..1141)
                     /gene="map"
                     /locus_tag="VKN64_01240"
     CDS             complement(392..1141)
                     /gene="map"
                     /locus_tag="VKN64_01240"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMA58884.1"
                     /translation="MIILKSPREINIMRDANKIVAETHEYLRELIKPGISTAEIDNKG
                     EEFIRKQGAEPSFLGYMGYPASVCVSINDEVVHGIPSKDRFLKEGDIVSLDIGAFYEG
                     FNGDAARTFPVGEISEEAKKLLKVTKEALMKGIEQALYKNRLSDISHAVQTHVENNNF
                     SVVREYVGHGIGRDMHEAPQIPNFGSPGHGPLLKEGMTLAIEPMVNVGDFKVKTLDDD
                     WTVVTEDGSLSAHFEHTIAINKNGPEILSKV"
     gene            complement(1138..1791)
                     /locus_tag="VKN64_01245"
     CDS             complement(1138..1791)
                     /locus_tag="VKN64_01245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMA58885.1"
                     /translation="MNLIMLGLPGAGKGTQAKKLSNKYNIPHISTGDIFRNAIQNETK
                     LGKKAKEYLDSGGLVPDELTNSIVRERLKEDDCKEGFILDGFPRTINQAEALEDMLKN
                     MNRELDLAIYIDVTREELIKRLTHRRTCSNCGAVYHLDYDPPEKEGICDKCGGKLIQR
                     SDDTEEVVKERLKINSKNIKNLIDFYEEEDKLATVKSDAGIEEVFEKIDEIVKEQAL"
     gene            complement(1813..3084)
                     /gene="secY"
                     /locus_tag="VKN64_01250"
     CDS             complement(1813..3084)
                     /gene="secY"
                     /locus_tag="VKN64_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMA58886.1"
                     /translation="MFSTFKNIFKVKELRKKVLFVLAMMMVYRLGAHIPVPGVDVGVI
                     QDTLFQGSGSGVLDYLDMFAGGALRNFTIFAMSITPYITASIILNLLTVVIPKLEELS
                     KQGAEGRKKIAQYTRYGTIVLAVIQGIGITSYINRFGAIPNPTFFDLFIIVVTLTAGT
                     AFLMWLGEQINEKGIGNGISIIIFTSIISRFPGQIYNTYNLFQAGSISILNILLFVAL
                     AVIIVAGIIFVQEGERRIPVQYSKRIVGRKVYGGRSTHIPMKVNQAGVIPVIFASSVL
                     LFPGVIAQVLPFDWAQNIAAMIQPGSTLYLILYALMILFFTYFYTAITFNPEEVAENM
                     QKNGGFIPGIRPGKATINYLDKILVRVTLAGAVFLTIVALLPFAMGPLTGVQIQFGGT
                     SVLIMTGVALQTMQQIESHLLMRHYEGFMKS"
     gene            complement(3086..3529)
                     /gene="rplO"
                     /locus_tag="VKN64_01255"
     CDS             complement(3086..3529)
                     /gene="rplO"
                     /locus_tag="VKN64_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMA58887.1"
                     /translation="MNLHDLKPAEGSKTRKRRVGRGTGSGRGYTSGRGANGQNSRSGS
                     KTRPTFEGGQTPLFRKLPKRGFTNIFKKQYSIVNVFQLNKFEEGTEITPELLKETGLI
                     DNIAKNGVKILAKGDINKNLTVKAQAFSSSAVEKIEDAGGKAEVI"
     gene            complement(3550..4050)
                     /gene="rpsE"
                     /locus_tag="VKN64_01260"
     CDS             complement(3550..4050)
                     /gene="rpsE"
                     /locus_tag="VKN64_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMA58888.1"
                     /translation="MSKIDPEKLDLDERVVNINRVSKVVKGGRNFSFSALVVVGDSNG
                     HVGAGIGKANEVPEAIRKGVDDAKKHLIEVPIVDTTIPHEITGVFGAGRVLLKPAVEG
                     TGVIAGGPVRAVVELAGINDILTKSLGSSNPINMVNATVTGLDELKSAEQVARLRGKS
                     VEEILG"
     gene            complement(4067..4423)
                     /gene="rplR"
                     /locus_tag="VKN64_01265"
     CDS             complement(4067..4423)
                     /gene="rplR"
                     /locus_tag="VKN64_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMA58889.1"
                     /translation="MDKKAARKRRHHRIRNKITGTPFKPRMVVNRSLKNMYVQIIDDL
                     SGETLASASTLDEQLKDDLENSGNVEAAKKVGELVAERALEKDIEEVVFDRAGYKYHG
                     RVKAVAEAAREKGLKF"
     gene            complement(4443..4973)
                     /gene="rplF"
                     /locus_tag="VKN64_01270"
     CDS             complement(4443..4973)
                     /gene="rplF"
                     /locus_tag="VKN64_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMA58890.1"
                     /translation="MSRIGKQPVDIPEKVDVNIDGNKVTVKGPKGEMTKEFDPNFDIN
                     IEDNAVVVKRPSDSKDDRSQHGLIRSLIEGMVEGVTEGFSKKLELVGVGYNAKVQGGD
                     LILEVGYSHPIKIEAPEGINFDVEKNVITVSGYDKQLVGDVAANIRETRKPEPYKGKG
                     VRYVGEHIRRKVGKTG"
     gene            complement(5017..5415)
                     /gene="rpsH"
                     /locus_tag="VKN64_01275"
     CDS             complement(5017..5415)
                     /gene="rpsH"
                     /locus_tag="VKN64_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMA58891.1"
                     /translation="MNITDPIADMLTRIRNANNVNKDVVDIPASNLKINIAKTLKDEG
                     FIKEYKIIDNYPQNKIRVYLKYGKDDEKVISGLKRISKPGLRVYVNKDEIPRVLGGLG
                     IAVISTSRGILTDKQARNEGIGGEVLCYVW"
     gene            complement(5438..>5560)
                     /locus_tag="VKN64_01280"
     CDS             complement(5438..>5560)
                     /locus_tag="VKN64_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HMA58892.1"
                     /translation="NRCSRCGRARGYMRKFDLCRICFRELAHKGEIPGVKKASW"
BASE COUNT         1583 a         1215 c          782 g         1980 t
ORIGIN      
        1 cgtcttatta ctttacattt atcacatata ggtttaacag atggtcttac cttcacctaa
       61 catccctcct taagcaaatt attttgcttt atgacggtaa gtaattctac cacgacttaa
      121 atcataaggt gaaagttcaa cggtcacttt gtcaccaggt aaaattctaa tatagttcat
      181 tctcatttta cctgaaacat gggccaatac ctcatgacca ttttctaact ctaccttgaa
      241 tttggcattc ggcaacggct cgataactgt accttgaact tcaataggtt cttccttagc
      301 catatatttg ataagcctcc ttatttttgt tacttaatta gcccaaattt tattatacca
      361 taaattacac gcttgaaaca aatctaaaca gttaaacctt acttaaaatt tcgggtccgt
      421 ttttatttat tgcaatagta tgttcgaaat gagctgataa gcttccatct tcggttacta
      481 ccgtccagtc atcatctaaa gttttgacct taaaatcgcc aacattaacc ataggttcaa
      541 ttgccagtgt cattccttct tttaacagag gaccatgtcc gggtgaacca aagttgggaa
      601 tttgcggagc ttcatgcata tctctaccga ttccatgtcc tacatattcc cttacaaccg
      661 agaaattatt attttctaca tgagtctgta ctgcatgtga gatgtcagat agtctattct
      721 tatataaggc ctgctctata cctttcatga gagcctcttt tgtgaccttt aatagttttt
      781 tagcttcttc agatatttct cccacgggaa atgtgcgggc agcatcacca ttaaagcctt
      841 cataaaaagc tcctatatca aggcttacga tgtctccttc ttttaaaaac ctgtctttgc
      901 tgggaattcc atgcacaacc tcatcattta tagagacaca aaccgaagca ggataaccca
      961 tatatcctaa aaaggatggc tcagctccct gtttgcgaat aaattcctcg cctttattat
     1021 caatttcggc tgttgaaatt cctggtttta ttaattcccg gagatactca tgtgtttcag
     1081 ctactatctt attggcatct ctcataatat ttatttctct cggtgatttt aagatgatca
     1141 taaagcttgc tccttaacaa tttcatcgat cttttcaaat acttcctcta tacctgcatc
     1201 actttttact gtagctaatt tatcttcttc ttcataaaaa tctatcagat tttttatatt
     1261 cttagaatta atcttgagtc tttctttgac aacttcttct gtatcatctg agcgctggat
     1321 caacttacca ccacatttat cacagatacc ttctttttca ggtggatcgt aatcaagatg
     1381 gtaaactgcg ccgcagttac tgcaggtcct tctatgagtt agtctcttta ttagttcttc
     1441 ccttgtaaca tcaatatata tagccaagtc aagctctcta ttcatatttt taagcatatc
     1501 ttctaaagct tctgcctgat ttatagttct ggggaatcca tctagaataa atccttcttt
     1561 acagtcatct tctttcaatc tttccctcac aatactgttt gttagctcat ccggtacaag
     1621 accaccggaa tctagatact ctttggcttt tttaccgagc ttggtttcat tttggattgc
     1681 atttctaaat atatctccag tcgaaatatg gggtatatta tatttattac ttaatttctt
     1741 ggcctgagta cctttaccag cacccggcaa tccaagcatt ataagattca tcttttatcc
     1801 cctcttcgtt ttttatgatt tcataaatcc ttcgtagtgt ctcattaata agtgtgattc
     1861 gatctgctgc atggtttgta gtgcgacacc tgtcataatc agaactgagg taccaccaaa
     1921 ctgaatttgc acacctgtta atggacccat tgcaaatggt aataacgcta caatagttaa
     1981 gaacactgca ccagcaagtg ttactctaac taatatttta tccaaataat ttatagtagc
     2041 tttacctggt cttattcctg gaataaatcc accatttttc tgcatatttt ctgctacctc
     2101 ttctggattg aaagtaatag cagtgtagaa atatgtgaag aataagatca ttaatgcata
     2161 aagaattaaa taaagtgttg aaccaggttg aatcatagct gcaatattct gtgcccaatc
     2221 aaatggtaac acttgagcaa ttacacccgg gaataataac acggaagacg cgaaaataac
     2281 cggtataact cccgcctggt taactttcat tggtatatga gtacttctac cgccatatac
     2341 ctttctacct acaattcttt ttgaatactg tacaggtatt cttctttctc cttcctgtac
     2401 aaagataata cccgctacga taattactgc taaagcaaca aataataata tgttcaagat
     2461 tgaaatactt cccgcttgaa aaagattata ggtattataa atttgacctg gaaatcttga
     2521 aattattgag gtaaaaatga taatagaaat accattacca ataccttttt cattaatctg
     2581 ctctccgagc cacattaaaa aggctgtacc ggcagtcaat gtaacaacaa ttataaataa
     2641 atcgaaaaag gtcggattcg gaatagctcc gaatctatta atataggatg taataccaat
     2701 accctgaata acagctaaaa caattgttcc atatctggta tactgagcga tcttctttct
     2761 accctcagca ccctgcttgg atagttcctc tagtttagga ataaccacag ttaaaagatt
     2821 aagaataatt gatgctgtaa tataaggggt gatactcatc gcaaagatgg taaagtttct
     2881 caaagcacca cctgcgaaca tatcaagata gtctagtact ccactacctg atccctggaa
     2941 aagagtgtcc tgaataactc ctacatctac cccgggtacg ggaatatggg cacctaatct
     3001 atagaccatc atcattgcta aaacaaataa tacttttttt cttaattctt taactttaaa
     3061 tatattttta aaggttgaaa acaacttata tcacctcagc ctttccaccg gcatcttcaa
     3121 ttttttccac ggcagatgaa ctgaaggctt gggctttaac agttaaattc ttgttaatat
     3181 ccccttttgc cagtatttta acaccatttt tagctatatt atctattaag cctgtttctt
     3241 ttagtaattc aggtgtaatt tcagttcctt cttcaaattt atttaattga aaaacattta
     3301 caatactata ctgtttttta aatatatttg taaaacctct tttaggtagt tttctaaata
     3361 aaggagtttg tccaccttca aatgttggtc tggtcttgga accagatctt gaattttgtc
     3421 catttgcccc gcgtccagat gtataacctc tacctgaacc ggtaccacgg ccgactcttc
     3481 tctttcttgt tttagaaccc tcagcaggtt ttaagtcatg taagttcata acgtactgca
     3541 cctccttaat tatcctagaa tttcttctac tgatttccct cttaatcttg ctacctgttc
     3601 agctgatttt aattcatcta aaccagtgac tgtagcgttt accatattaa taggattaga
     3661 agaaccaagt gattttgtta aaatatcatt gataccagct aattctacaa ccgcacgaac
     3721 aggtccaccg gcaattacac cagtaccctc aacggctggt tttaataaaa ctcgccctgc
     3781 accaaatacc ccagtaatct catgtgggat ggtggtatca acgattggta cttctattag
     3841 gtgttttttt gcatcatcta caccttttct gattgcttct ggtacttcgt ttgcctttcc
     3901 aatacctgct ccaacatgac cattggaatc tccaacaacc acgagagcag agaaactaaa
     3961 gttacgtcca ccttttacaa ctttagatac acgatttata tttactactc tttcgtctaa
     4021 atccaatttt tctggatcaa tcttgctcat ggattcgcct ccttcgttaa aattttaatc
     4081 ccttttctct tgctgcttca gctactgctt tgacgcggcc gtgatattta tatccagctc
     4141 tgtcaaatac aacttcttcg atatcttttt caagagctcg ttcagcaact aattctccta
     4201 cttttttagc agcttcaaca ttaccgctgt tctctaaatc atctttgagt tgttcatcca
     4261 aagtagaagc ggatgcaaga gtttctccgg ataagtcatc tattatttga acatacatat
     4321 ttttcaaact cctatttaca accatgcgtg gtttaaaagg agtacctgtt attttatttc
     4381 ttattctatg gtgccttctc tttctggcag ccttcttatc cataattaat cactcccttc
     4441 aattaaccgg ttttaccaac ttttcttctt atgtgttcgc ctacatatct tactcccttg
     4501 cccttataag gttcaggttt tctagtttct cttatatttg ctgcgacatc accgactaac
     4561 tgtttatcat aacctgatac agtgataaca ttcttttcta catcaaagtt aattccttca
     4621 ggtgcttcaa tcttgattgg atgtgaatat ccaacttcta aaattagatc tccgccttgt
     4681 acttttgcgt tataaccaac accaactaat tctaactttt tagaaaaacc ttctgttaca
     4741 ccttcgacca taccctcgat taagctgcgg attaaaccgt gctgagagcg gtcatcttta
     4801 gaatcagatg gtctttttac cactacagcg ttatcttcta tatttatatc aaaattagga
     4861 tcgaattctt ttgtcatttc acctttaggt cctttaacag ttactttatt tccgtcaatg
     4921 ttaacatcaa ccttttccgg gatgtcaaca ggttgtttac ctatacggga catacaattc
     4981 acctccagct atttttgctt taaataagat tatatcttac catacatagc agagaacttc
     5041 tccacctatg ccttcattgc gagcttgttt atctgttaag attcctcggg atgttgagat
     5101 aacagctata cctaatccac cgagtactct tggaatttca tctttattta cataaactct
     5161 taagcctggt ttactaattc tttttaagcc actaataact ttttcgtcat ctttaccata
     5221 cttcagataa acccttatct tattttgagg ataattatct ataattttat attcctttat
     5281 aaaaccttca tcttttaaag ttttggcaat atttatcttt aaattcgaag caggtatatc
     5341 aacgacatct ttatttacat tattggcgtt ccggatacga gttaacatat ctgcaatcgg
     5401 atcagtaata ttcattaagc catcctcctt cctgatttta ccaacttgct tttttaactc
     5461 caggtatttc acctttatgt gctaattctc tgaaacaaat tctacagaga tcaaattttc
     5521 tcatataacc tcgagctctt ccacatctac tacatctatt
//
LOCUS       DATGCJ010000029         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_48244, whole genome shotgun sequence.
ACCESSION   DATGCJ010000029 DATGCJ010000000
VERSION     DATGCJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48244"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VKN64_01285"
     CDS             complement(<1..498)
                     /locus_tag="VKN64_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA58893.1"
                     /translation="MCQSNYNTDKGKGKHLTFDDRKLIAHLFNYQNKNYTEIGEELNS
                     HRTTISREIKNGLITLKRAQDSYKEVYYYEIAQQVHDNNATAKGPNLKIDKNHEIAQF
                     IEKKIGEKYSPEVIAELIKDDDRFDMCLSYKTIYNYIDMGVLMIDRDDLVYGNYSNDS
                     NNRRKE"
     gene            813..1541
                     /gene="nagB"
                     /locus_tag="VKN64_01290"
     CDS             813..1541
                     /gene="nagB"
                     /locus_tag="VKN64_01290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HMA58894.1"
                     /translation="MRIIKVKNYEALSIKAARIVAGQIELKPNLVLGLSTDSTSQGMF
                     ENLTKIHKQDNLSFNYIKVFKLDEFVGIKKEHPGSLNTYIQREFLDKVDIRTENFHTP
                     NGYAEDIKKECHRYENKIKEVGGIDFLVLGIGENGNIAFLEPGNSLPLKTSTIKLSEK
                     TRETHSSNFDSLKEVPTKAITMGLDTILSADEILLLASGKSKAEAVKKMLSREVSIKT
                     PASLLNLHRNITVLIDKQAGKNIK"
     gene            2023..3435
                     /gene="nhaC"
                     /locus_tag="VKN64_01295"
     CDS             2023..3435
                     /gene="nhaC"
                     /locus_tag="VKN64_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HMA58895.1"
                     /translation="MRREKISFFTALLPIIILLLTGAFSVFYWKVGMIVPLLSGVITT
                     GIIGFVHNFDWEELQEGLKEGVANALPAIFILLIVGSIIGTWILSGTIPAMIYYGLKF
                     LSPGLFVPLAALITAIVSTTLGTSFTSIATVGIALIAVGNAMGFATPLTAAAIISGAF
                     FGDKLSPLSDTTNIAAAMADCELFEHVGHMLWDTIPAFAISLVIYWGVGQSQMSGGFE
                     GTEQINTLMQGIDGLFNITPVLFILPLITIILAVRKTPAIPALLSMSILGGVFAVLFQ
                     GAGLAAVLGAFTSGFKSSSEIEMINSLLSKGGVTSMGGTIILLITAAALGGIMQKVGI
                     LDIILERMMTWINSKRKMLITSLVTSFLIGLSTGAQVLAIIIPANMFSNTFKKMGLHT
                     KNLSRNVEAGGTVGITLVPWSVPAIFAASMLGAQPAQFIPFLFFPMLVILINAFYGLT
                     EISIAEIEPVTEKEKVRREA"
     gene            3435..4316
                     /locus_tag="VKN64_01300"
     CDS             3435..4316
                     /locus_tag="VKN64_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA58896.1"
                     /translation="MSDIIIKVLGTAQDGGYPQPDCDCPNCIRVRKNRNLIRYPASLA
                     VLNKKEKSTYLIDPTPQLPAQLEELNQMAKKLNFPDDHLKEIIITHAHIGHYTGLMYF
                     GKEAINSSGLKVRGSSRLKEFLTTNQPWQSLVENNNIIINIFIFGQDIYSDKNFNIEP
                     IKVPHRAEFTDTAGFLIKGQNKSLFYLPDIDSWEGFRPKFADLSSKTDYMVIDGTFYT
                     KEELGELRGRNIDNVPHPPIEDTINELLENKLEKNESEVYFTHFNHTNKVLDPNFTND
                     FKEDNIFFLSEDMNFEL"
BASE COUNT         1389 a          712 c          795 g         1456 t
ORIGIN      
        1 ttcttttctc ctgttattac tatcattgct gtaattacca tatactaaat catctctatc
       61 tatcatcaat actcccatgt ctatataatt atatatcgtc ttataggata aacacatatc
      121 aaaccgatca tcatctttaa ttaactcagc tattacttcc ggggaatact tttcacctat
      181 tttcttttca ataaactgtg ctatttcatg gtttttatct atctttaaat tgggtccttt
      241 agctgtggcg ttattatcat gaacctgttg ggctatctca taataataaa cctccttata
      301 actgtcttgt gcgcgtttaa gagtaattaa accattttta atttccctgc ttattgtagt
      361 ccgatgactg tttaattctt ctcctatttc ggtataattc ttattttgat agttaaaaag
      421 atgggcaatt aactttctat catcaaatgt taaatgtttt cctttgcctt tatctgtgtt
      481 ataattactt tgacacatgt cggtgcctcc ttgttttgtt ttgtggtgtt actcaacatt
      541 ataaaggagg ttaccggtat gtgtcacatt tttatttacc ttgtgtgcat ttaattatac
      601 aattcgggtt gttttaaaat agctttaatt tttcaattca gattcgactt gagaagcaaa
      661 aatatgttat actagcagtg gttattcgtc gagcctttta ttgttataga aaaattttta
      721 atgttctatt ccatttaaga aataactttt tatttaaatt ttcaaccaat tatcaagtac
      781 tatctataaa ttttcaaagg aggtttcaac aggtgagaat tataaaagta aaaaattatg
      841 aagcactcag tataaaagca gctagaatag tggcagggca aattgagtta aagcccaatc
      901 ttgtattggg actatctacg gatagtactt cccagggtat gtttgaaaat ttaactaaaa
      961 tacataagca agataattta agtttcaatt atattaaagt ttttaaatta gatgaatttg
     1021 tcggtattaa aaaggaacat cctggtagtt taaacacata tatacaaaga gagtttttag
     1081 ataaagtgga tattagaaca gaaaactttc atactccaaa tggatatgcc gaagatatta
     1141 aaaaggaatg ccaccgatat gaaaataaga taaaagaggt gggtggaatt gattttcttg
     1201 tcttgggaat aggtgaaaat ggaaatattg cttttttaga acccggtaat tcattaccac
     1261 taaaaacatc tactataaaa ttgagcgaaa aaactagaga aacccactcc agtaattttg
     1321 atagtttaaa agaggttccc actaaagcaa ttacaatggg ccttgatact attctttcag
     1381 cagatgaaat attacttttg gctagtggta aatctaaagc tgaagctgtc aaaaaaatgt
     1441 taagcagaga agtttctatt aaaacaccag cttcactttt aaatctacac cgtaatataa
     1501 ctgttttaat agataaacaa gctgggaaaa atattaaata ataatcataa gtaggaaata
     1561 taaattaata ttgtaaagat taaaaaataa aaaagccttt tacgatgtaa cattttttat
     1621 ttaccttgtg tgcatttaat tatacaattc agttttttaa aaatttaaaa gtaacttgac
     1681 actatcgttt ccagattaaa tcttgacatt aaagaaggta ttatatataa ttattccaaa
     1741 ttaatccaaa taataaaaaa ctatgactgg ggaagtagta agcttaaggc cgaaatacac
     1801 ccctgagttg ccggctgaac taagagtaag ctaggccgtt atccaacgtt atccggtgag
     1861 agagtgctag ctctcaagag agttgttcta accatcttat tatttgagtt agaataaatt
     1921 caggtggtac cgcgagcatg tcgtcctgat atagggacgg cttttttgtt tttctatagg
     1981 tttttattat ttgtaaaaaa atttcaagga ggagtattaa aaatgagaag agagaagatt
     2041 tcctttttta cagctttatt accgattatt atcttattat taacaggtgc cttttcagtt
     2101 ttttactgga aggtcggcat gattgttcct ctacttagtg gtgttataac aactggcatt
     2161 ataggctttg tacataattt tgattgggaa gaactgcagg agggcttaaa agaaggggta
     2221 gcaaatgccc tacctgctat ctttatctta ttgattgtag gttctatcat agggacctgg
     2281 atcttaagcg gcacaattcc cgccatgatc tattacggac ttaaattttt aagccccggt
     2341 ttgtttgttc ctttagcagc cttaattaca gctatcgttt ccacaacctt aggtacatcc
     2401 tttacttcaa ttgctacggt cggtattgct ttaattgctg tcgggaatgc gatgggtttt
     2461 gctacacctc tgaccgctgc cgctataatc tcgggagcat tttttggaga taagttatct
     2521 cccttatctg ataccaccaa tatcgccgcc gccatggcag actgtgaatt gtttgaacat
     2581 gttggacata tgctctggga tacaattccg gcctttgcga tttccctggt catttactgg
     2641 ggagtggggc agtcccagat gagcggtggt tttgaaggaa ctgaacagat taatacctta
     2701 atgcagggca tcgatggtct atttaatatt acaccagtat tatttattct acctttaatt
     2761 acaattattc tggcagttag aaagaccccg gctatccccg ctttactttc gatgtctatt
     2821 ttagggggag tttttgccgt gctttttcag ggagcaggcc tggctgctgt cttaggagcg
     2881 tttacctctg gttttaagag cagcagtgag attgagatga tcaacagcct gctctctaaa
     2941 ggtggagtta cttccatggg cggcacgatt atcttactta tcaccgcggc tgccttaggt
     3001 gggataatgc agaaagtagg tattttagat ataatattag aaaggatgat gacctggatt
     3061 aacagtaaaa gaaagatgtt aatcacttct ctcgtaactt cctttttaat cggtctaagt
     3121 acgggagcac aggtcttagc tattatcatc cctgctaata tgttttctaa taccttcaaa
     3181 aagatgggat tgcacacgaa aaatctaagc aggaatgtag aagccggagg aacagtcggt
     3241 ataaccttag ttccctggag tgtacccgct atctttgcgg ccagtatgct gggagcccaa
     3301 ccggcccagt ttataccatt cctctttttc ccgatgttag tgattttaat taatgctttt
     3361 tacggtttaa cagagatttc aatagctgaa attgaacctg taactgaaaa agaaaaggta
     3421 aggagagaag cctaatgagt gatattataa taaaagtatt gggaacagct caggatgggg
     3481 gatatcccca gccggactgt gactgtccga actgtataag agtcagaaag aataggaact
     3541 taattagata tcccgcttca ttagctgtat taaataaaaa agaaaagtca acttatctaa
     3601 ttgaccctac accacaattg ccagcccaat tagaagaatt aaatcagatg gctaaaaaat
     3661 taaattttcc ggatgatcat ttaaaagaga ttataatcac ccatgcccat ataggccatt
     3721 ataccggtct aatgtacttt ggcaaggaag ctattaacag tagtggttta aaggttaggg
     3781 gtagttcacg cttaaaggaa tttttaacaa ctaatcagcc ctggcagagt ttagttgaaa
     3841 acaataatat tataatcaat atatttatct ttggacagga tatttatagt gacaaaaact
     3901 ttaatattga gcctattaaa gttccccatc gggcggagtt caccgatacc gctggatttt
     3961 taattaaagg tcagaacaaa tcacttttct atttgcctga tattgatagt tgggagggat
     4021 tccgaccaaa atttgctgat ctaagttcca aaactgatta tatggtcatt gatggtacct
     4081 tctatactaa agaagaatta ggtgagttaa ggggcagaaa tatcgataat gtacctcacc
     4141 ctcccattga agacacaata aatgagttgt tagaaaacaa actggagaaa aatgaaagtg
     4201 aagtatattt cactcacttt aaccatacaa ataaggtctt agatcctaat tttaccaatg
     4261 attttaaaga agacaatatt ttctttttaa gtgaagatat gaatttcgag ttataaatta
     4321 aaaataatta ctgttaatat ttgaaagttg ag
//
LOCUS       DATGCJ010000030         8326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_226385, whole genome shotgun sequence.
ACCESSION   DATGCJ010000030 DATGCJ010000000
VERSION     DATGCJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226385"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..513
                     /locus_tag="VKN64_01305"
     CDS             178..513
                     /locus_tag="VKN64_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HMA58897.1"
                     /translation="MNTQEKEALLQDIYERGFQYEQKYGFCTQAVIGALQDFFAGIDD
                     EVFKATHPLAGGGALCGDGTCGALVGGLAVVGSFFGRSRDEYAESDPDGWMTSCAIAK
                     EIREKFLTE"
     gene            complement(986..3565)
                     /gene="clpB"
                     /locus_tag="VKN64_01310"
     CDS             complement(986..3565)
                     /gene="clpB"
                     /locus_tag="VKN64_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HMA58898.1"
                     /translation="MRMEDLTRKAQEGLAEAQHLAEDKGNQEVYPAHLFLALVEDDQG
                     IMVPLLEKLGINLKVFRKDLNEIIEKLPKVYSEQGQLYMSQELNNVLRKARKEAKNLG
                     DEYISTEHFLLGLLSGGKGKTYDYFRSKNIDLEKAREAIENIREGAKVTSQDAEEQYQ
                     VLEKYTIDITELAREGELDPVIGRDERIRRIMQVLSRRRKNNPVLIGEPGVGKTAIVE
                     GLAQRIVNGDVPEALKDKKVIKLDMGSLVAGTKFRGEFEDRLKSVLKEIRKAEGQIIL
                     FIDEMHTIVGAGAAEGAMDASNLLKPALARGELHCVGATTLNEYKKHIEKDAALERRF
                     QPVKISEPSVEDTISILRGLKEKYEIHHGVRIQDSALVAAANLSERYLTERFLPDKAI
                     DLVDEAASKLRIDIDSMPLEIDELNRRIRRLETEKEALKQEEDKESQERLEELIDKIQ
                     ELKEKRDPLLASWDNEKELIQKRQNLNEEIEKVKVKAENAEREANYEEAARLRYGELN
                     DLQKELEETKEKLENMEGDFNVLKEEVTAEDIAEIVSSWTDIPVQELMEDEREKLVHL
                     EEELSKRVVGQDEAITAVSNAIRRSRTGLQDVDRPLGSFIFMGPTGVGKTELAKTLAA
                     YLFDDEKNLVRLDMSEYMERHAVAKLIGSPPGYVGFEEGGQLTERIRRQPYSVILLDE
                     IEKAHQDVFNILLQILDDGILTDSQGNEVNFKNTVIIMTSNIGSEYIQDLDDEEEMRK
                     RVTEALKGNFRPEFLNRVDEKIIFHSLSKENLLDIVDIKLDDLRDKLEERNLAIEVTE
                     DAKEEIMELGYDPTYGARPINRTIQKYLKDPLAMKMLEDEIQEGAEVLIDFDGDKFQF
                     KVQ"
     gene            3764..4105
                     /locus_tag="VKN64_01315"
     CDS             3764..4105
                     /locus_tag="VKN64_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58899.1"
                     /translation="MSNGYNEFIYKKPDNTGMLIRYKEQENSIDFIVFEVEFADFDMN
                     EEGNVTNYTIENEKELYECNINRAGKSHFHFAHGIEFESPKDYGAFVDVLNQLYGLAE
                     QLLGEKAEFKW"
     gene            complement(4199..4519)
                     /locus_tag="VKN64_01320"
     CDS             complement(4199..4519)
                     /locus_tag="VKN64_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA58900.1"
                     /translation="MSVIKLENLQAEENMRGIMTRNIHKNENVQVKNLILEEGDVVPA
                     HQVPVDVFFHVMEGKGSIMIGDETYQLDEKDFITCPPNTKMKVMADRGDKFNILNVKT
                     PALN"
     gene            complement(4526..5284)
                     /locus_tag="VKN64_01325"
     CDS             complement(4526..5284)
                     /locus_tag="VKN64_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HMA58901.1"
                     /translation="MTDKKERLKEILERLNNDEDVEEVKKEARELIDEISAKDLSEAE
                     QQLINEGLEETKLRHLCAAHLEVMADELEEMMGDLDESHPLAVLVREHDEILNTLDRL
                     EIAVDKIDKLDEYDPDKEVFEELKEIGQLLLETEKHHEREEEALFPEVDALGITGPTR
                     IMRMEHDDLWPYKEEINELAENADQMDFAEFKSQLKELSEFLVLTLRDHIYKENNILY
                     PTAKDVIHYDKWEKIQNLSDEIGYCSFTTFKEEE"
     gene            complement(5464..6723)
                     /locus_tag="VKN64_01330"
     CDS             complement(5464..6723)
                     /locus_tag="VKN64_01330"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMA58902.1"
                     /translation="MQKELPFNKERLEEIIDKYPTPFHIYDEKAIRENARRFLKAFEW
                     NEGFEEYFAVKATPNPFILKILKEEGFGTDCSSLTELILSEEVGITGEDIMFTSNNTP
                     AEEYEKARELGAIINLDDISHIPFLAKHGGIPELISLRYNPGSRRAGSFIMGDPSEVK
                     YGFTKEQIFEGLADLKHRGVERFGLHTFIASNELNPQYFVDTAAMMFDLAAEVKERLD
                     INLEFLNIGGGIGIPYRPEEEPVDLEYVGAGIKEKYDEIIKANNLDPLAIFMECGRMI
                     TGPYGYLVSRVIHKKNIYKKHVGLDASMNDLMRPGMYDAYHHVTVMGKEDEPKEYTYD
                     VTGSLCESIDRFAVDRQLPKIDVGDIVVIHDTGAHGHAMGFNYNGKLRGGELLLRTNG
                     EVEEIRRSETIEDYFATLDFDKLKDFE"
     gene            complement(6742..8130)
                     /locus_tag="VKN64_01335"
     CDS             complement(6742..8130)
                     /locus_tag="VKN64_01335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006614.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMA58903.1"
                     /translation="MLTVEKIGGSSMSEFQSVLNNIIGQPDSERFSYNRVFVVSAYAG
                     VTDLLLEDKKNSEPGIYQSFINDDGTYKAKLDELNYRLTDINKKFADLGLDVKEADRF
                     IKKRIEHTKKYLDSMEEILSSGYIKEANIYSAAREILTSLGEAHSAYNSHIILSNLGI
                     NSQFVDLTGFYDRTQLTMEERIKKSFDDIDLFENIVIAPGYTKDKGGMITQFGRGYSE
                     ITFSRIATFLNADQAIIHKEHHLSSADPEIVGKEKAIPVGRTNYNVADQLADIGMEAI
                     HPKASKPLELAGIKLIVKNTFEPEHPGTIITKDFVVEDSKIEIIAGSKEMVVIEVHDT
                     SMVGEVGFDYEIMQILKKHNISYVMKATNANSISQVIWEKDLSEDLVNELKDKYTDIK
                     IKEIAMVCLIGSNISKPNILARAAQVLGENGININCVSISLSQVNIQFVVNRENYIES
                     IKLLNDNLMFEK"
BASE COUNT         2427 a         1648 c         1274 g         2977 t
ORIGIN      
        1 gcatgtgtaa taaatatgta gtctattata caatttatca ttttttgtaa tatttttatt
       61 actttttgtg tttatagtaa ataaaaatta ttcattattg tgctattaga gtttttgaca
      121 tatgataata atactgttaa aattaaagtg aattatatca catttaggag gtttaatatg
      181 aatacacaag aaaaagaagc tctcttacag gatatttacg aaagaggctt tcaatatgaa
      241 caaaaatatg ggttttgtac tcaagccgtg attggcgcct tacaggattt ctttgctggt
      301 attgatgatg aggtttttaa agctacccat cctctagcag gtggtggagc cctctgtggt
      361 gatggaactt gtggggccct agttggtggt ttagctgttg taggaagttt ttttgggaga
      421 agtagagatg aatatgcaga atcagacccg gatggttgga tgacttcttg cgctattgct
      481 aaagaaatca gggagaagtt cctaacagaa taaggcagtg taatttgtgc tgacgtccag
      541 gctgaaaaaa tgggtaggag ttttgattta tgggatgaag aagattttaa aagtttgaag
      601 aagctggagc ccatgaagat caatgccttg aaataacagg gaaaacagca aaatggacag
      661 ctgaaattct gcttgcacac agcgtaaaac ctaaaaagac acctgatccg attcatgcaa
      721 aataaaagaa ttagatagaa caaaaaataa aatgaatgtt agctaatact ttatcagagc
      781 ccaatctttg ggctcttttt acgttttatt tataattatt atttaaatta gatatgatca
      841 tttctaatat catatgtaat tttaatccca acatttttta atcaatcaat aaatgtacag
      901 ttcggtaaat aaaaaccctt aaggctgata gcttaaacca tccaccttaa gggtttaaat
      961 caaaggaggt atttatatta tctacttact gtactttaaa ctggaactta tctccgtcaa
     1021 agtcgattag aacctcggct ccttcttgaa tttcatcttc tagcatcttc atagcaagcg
     1081 gatcttttag atatttttga atcgtcctat taattggcct tgccccatag gttggatcat
     1141 atcctaattc cataatctct tctttagcat cttcggttac ttctatggct agattgcgtt
     1201 cttctaattt atctcttaag tcatctaatt taatatcaac aatatcaagt aagttttctt
     1261 tacttaagga atggaatatt atcttttcat cgactctatt taaaaattca ggtctaaagt
     1321 taccttttag tgcttctgtt actctttttc tcatctcttc ttcatcatct aagtcctgga
     1381 tatattcaga cccaatattt gaagtcataa ttattacagt atttttaaag ttaacttcat
     1441 tcccctgact gtcagttaaa ataccatcat ctaagatctg taataagatg ttgaagacat
     1501 cctggtgagc tttttcaatt tcatctaata agatgactga ataaggttgt cttctgatcc
     1561 tttctgttaa ctgaccgcct tcttcaaaac ctacataacc cggtggggaa ccgattaact
     1621 tagcaacagc atggcgttcc atatattcag acatatcaag tctaactaaa tttttctcat
     1681 catcaaagag ataagctgct agcgttttag caagttcagt tttaccaact ccggtcggtc
     1741 ccatgaagat aaaagaacct aagggacggt caacatcctg tagaccggtt cttgatcttc
     1801 taatcgcatt acttactgcg gtaattgctt catcctgacc aacaacccgc ttagatagct
     1861 cttcttctag gtgaactaac ttttctcttt cgtcttccat caattcctgt acaggtatat
     1921 ctgtccagga tgagacgatc tctgctatat cttcagcggt aacttcttct tttaaaacgt
     1981 tgaagtcacc ttccatattt tctaattttt ctttggtttc ttctaattct ttttgcaggt
     2041 catttaattc tccatatctt aaacgggccg cttcttcata gtttgcttct ctttctgcat
     2101 tttcagcctt aactttcact ttttcaatct cttcatttaa attctgcctt ttttgaatca
     2161 attctttctc gttatcccag cttgctaaaa gcggatctct tttttcttta agttcctgta
     2221 ttttatcaat cagttcttct aatctttcct gagattcttt gtcttcttcc tgttttaaag
     2281 cttccttttc cgtttcaagt cttctaattc ttctatttag ttcatctatc tctaaaggca
     2341 tagagtctat atcgattctt aacttagaag cagcttcatc cactaagtca atcgctttat
     2401 caggtaagaa tctttctgtt agatatcttt ctgacaggtt ggcagctgcc actaaagcac
     2461 tgtcctggat tctaaccccg tggtgtattt catatttctc ttttaaaccc cgcaagattg
     2521 atatcgtatc ttcaacagaa ggttcagaaa tcttaacggg ttggaacctt ctttctaagg
     2581 cggcatcttt ttcaatgtgt tttttgtatt catttagggt agttgcgcca acacagtgca
     2641 gttctccccg ggcaagggca ggttttaaca gattagaagc atccattgcc ccttcggctg
     2701 caccggctcc tacgatagta tgcatctcat cgataaatag gataatctgt ccttctgctt
     2761 ttctaatttc ttttagtaca gactttaatc tatcttcaaa ttcacctcta aatttagttc
     2821 ctgccactaa tgaacccatg tcgagcttaa taactttctt gtctttaagt gcttcaggta
     2881 catcaccatt cacaattctt tgggccagac cttctacaat tgcggtttta ccaacaccgg
     2941 gttcaccgat taagaccgga ttgttcttcc ttcttctaga aagaacctgc atgatccttc
     3001 tgatcctttc atcacggccg attacgggat ctaactctcc ttcgcgggcc agttcagtaa
     3061 tatctatcgt atacttctca agtacctgat actgttcttc agcatcctga gaagttactt
     3121 tcgcgccttc tctaatattc tcgattgctt ctctagcttt ctctaaatca atattttttg
     3181 atctgaagta gtcataagtc ttacctttac cacctgataa aagtcctaat aagaaatgct
     3241 cggttgaaat gtattcatca ccgagattct tcgcttcttt ccgggctttt ctaagtacat
     3301 tatttaattc ttgagacatg taaagctgtc cctgttcaga atagactttc ggaagttttt
     3361 caataatttc gtttagatct tttcggaata ctttcaggtt aattcccagt ttttctagta
     3421 agggaaccat tataccctgg tcatcttcca ctagcgctaa aaataggtga gcaggataaa
     3481 cttcctggtt acccttatct tctgcaagat gctgtgcttc agctaaacct tcctgtgctt
     3541 tacgagtaag atcttccatt cgcatctgtt gatccctcca atcaatatta ctatatcaat
     3601 tatttccttt tatattatac aacaaattta tactaaagtc aaagaaggtc aaatttttta
     3661 atgaaaaata taacccactt ctataaaggt atataacctg atatatataa ttataaaagt
     3721 gtgttagctt tcaaatctaa ataaatctta ggaggtttta ataatgtcaa atggatacaa
     3781 tgaattcatt tataaaaagc ctgataatac tggtatgttg ataagatata aagaacagga
     3841 aaattcaatt gactttatag tctttgaagt tgagtttgct gattttgata tgaatgaaga
     3901 gggaaatgta acaaactata ctattgaaaa cgaaaaagaa ttatatgaat gtaatattaa
     3961 ccgggcaggt aagtcccact ttcattttgc acacggaata gaatttgaat caccaaaaga
     4021 ctatggtgct tttgtagatg tattaaatca gctctatgga ttagcggaac aattattagg
     4081 agaaaaagca gaatttaaat ggtaataatc ataaaattaa tctgctatat taaaatttat
     4141 aagtacataa aaaaacacac agggggaggc ctgtgtgtca aaaaacatta tgtgtttttt
     4201 agttcaaagc aggggttttg acattcagta tattaaattt gtcgcctctg tctgccataa
     4261 ctttcatctt agtattagga ggacatgtta taaaatcttt ttcatccaat tggtaggtct
     4321 catctccgat cataatggaa cctttacctt ccattacatg gaagaagaca tcaaccggaa
     4381 cctgatgagc aggaactacg tctccttctt ctaagatcaa gttctttacc tggacatttt
     4441 catttttgtg tatatttctt gtcataattc ctctcatatt ttcttctgct tgtaagtttt
     4501 ctagtttaat tacgctcatc tttaattact cctcctcttt aaaagttgta aatgaacagt
     4561 atcctatctc atcacttaag ttctggattt tttcccactt gtcataatgg atgacatctt
     4621 tggcagtggg atatagaata ttattttcct tatatatatg atctctcaaa gtcagaacta
     4681 aaaactctga gagttctttt agctgactct taaactctgc aaagtccatt tgatcagcat
     4741 tttcggccag ttcatttatc tcttccttat agggccagag atcatcatgt tccattctca
     4801 ttattctggt tggtccggta atacctaaag catcaacttc cgggaagaga gcttcttctt
     4861 ctctttcatg atgcttttca gtttctaata acaattgccc tatttctttt aattcttcaa
     4921 acacttcttt atcagggtca tattcatcta acttatcaat cttatcaact gctatttcca
     4981 atctatctaa tgtatttaat atctcatcgt gttctcttac caaaacagcc aacggatgag
     5041 attcatctaa atcacccatc atttcttcta gttcatctgc cattacttcc agatgggcag
     5101 cacagaggtg tcttaacttg gtctcttcta aaccttcatt aattaactgc tgttctgctt
     5161 ccgagagatc tttagcagaa atttcatcta ttaattcacg cgcttctttt tttacttcct
     5221 caacatcttc atcattattt aatctttcta aaatctcttt taaacgttct tttttatcag
     5281 tcataaattt ttcctcctaa acttgattaa ttctatcaac tttatttact ttaattatat
     5341 tataataaaa gacttaatgc aattctgtga tatatatcat ataaagaagg aaaaatatta
     5401 aaaaccctgc cttaatttaa tatcaaagca gggttaagta tactatatta tcttttttta
     5461 tttctattca aaatctttta acttatcaaa atccagagta gcaaagtaat cctcaatcgt
     5521 ttctgacctt cggatttctt ctacttcacc attagttcga agcagtagct cgccgcctct
     5581 cagtttacca ttatagttaa agcccatcgc atggccgtgg gctccggtat catggatgac
     5641 aactatatct cccacatcta tttttggtag ctgcctgtct acagcaaacc tatctatgga
     5701 ttcacagagc gagcctgtaa catcataagt atattctttg ggttcatctt ctttacccat
     5761 gactgtcacg tgatggtagg catcatacat accgggtctc attaaatcat tcatgctggc
     5821 atcaagaccc acgtgttttt tatagatgtt ctttttatga attacccgtg aaactaaata
     5881 gccgtaaggc cctgtaatca tccgtccaca ttccatgaaa atagccaaag gatctaaatt
     5941 attagcttta ataatttcat catacttctc tttaataccg gccccaacat attctaaatc
     6001 aaccggttct tcttccggac ggtaaggtat gcctattccg cccccaatat ttaaaaattc
     6061 gaggttgata tcaagtcttt ctttaacttc tgccgccaaa tcaaacatca tggcagctgt
     6121 atcgacaaag tactgtggat ttaattcatt agaagcaatg aaagtgtgta atccaaacct
     6181 ttcgacaccc cggtgtttta aatcagctaa tccttcgaag atctgttctt tggtaaagcc
     6241 gtactttact tccgaaggat ctcccatgat aaaactgccg gctctccggc tgcccggatt
     6301 gtatcttaaa gatattagtt cgggaattcc cccgtgttta gctaagaaag gaatatgtga
     6361 aatatcatct aagttgatta ttgctcccaa ttcccgggcc ttttcatatt cttcagccgg
     6421 ggtattattg gaggtgaaca taatatcttc tcctgtaatc ccgacctctt ctgacaatat
     6481 tagttcggtt aaagaactac agtcggttcc aaaaccctct tcctttaata tttttaaaat
     6541 aaagggattg ggagttgctt taacagcaaa gtactcttca aaaccttcgt tccattcaaa
     6601 cgcctttaaa aatctccttg cattttcacg aattgccttt tcatcataga tatgaaaagg
     6661 agtcggatat ttatctataa tttcttctaa tctttcctta ttaaaaggaa gttccttttg
     6721 catttttttc accaccaaat attatttttc gaacattaga ttatcattta atagtttaat
     6781 tgattctata tagttttctc tatttacaac aaactggata ttaacctgac ttaaagaaat
     6841 tgaaacacag tttatattaa taccgttttc acccaagacc tgagctgctc tagccagtat
     6901 attaggttta ctaatatttg aacctattaa acagaccatg gcaatctctt tgatcttgat
     6961 atcagtatat ttgtccttaa gttcattgac cagatcttca cttaaatctt tttcccagat
     7021 cacctgggaa atactgttgg cattggttgc tttcattaca taactaatat tatgtttttt
     7081 tagaatctgc attatttcat agtcaaaacc gacttctccc accattgagg tgtcatgtac
     7141 ttcaatgacc accatctctt tggaaccggc aataatctca atcttggaat cttccacaac
     7201 aaaatctttc gtaataattg taccggggtg ttcgggttca aaagtattct tgacaatcag
     7261 ttttataccg gccaactcca gaggtttgga agccttaggg tgaattgcct ccattccgat
     7321 atcagctaac tgatcagcca cattataatt tgtccttcca actggaatag ctttttcttt
     7381 ccccactatt tcaggatcag ccgaagatag atggtgttcc ttatggataa tcgcctggtc
     7441 ggcattcaaa aaggtggcga ttctcgaaaa ggtaatttca gaataaccgc gtccgaactg
     7501 ggtgatcata ccacccttat ccttagtata accgggtgca atcacaatat tttcgaataa
     7561 atcaatatca tcaaatgatt ttttgattct ttcttccata gtcagctggg tcctatcata
     7621 aaaaccggtt aaatcgacaa attgagaatt aatccctaaa ttcgatagta taatatgaga
     7681 attgtaggca gagtgagctt ctccgagaga agttaaaatt tctctggccg cagaataaat
     7741 attggcttct tttatatagc cggaagacaa gatttcttcc atactatcta gatacttttt
     7801 agtatgctct attctctttt taatgaaacg gtcggcttcc ttaacatcta gtcccagatc
     7861 agcaaatttc ttattaatat cggtcaatct ataatttaat tcatctaatt tagctttata
     7921 ggtaccatca tcattgataa aactttgata gatgcctggt tctgaatttt ttttgtcttc
     7981 taatagcaaa tcagtcacgc ccgcataggc agaaactaca aatactctat tatagctgaa
     8041 acgttcagaa tcaggttggc cgataatatt atttaaaact gattgaaact cgctcatgga
     8101 tgaaccgccg attttttcta ctgttaacac aataaatact cccttctata tacttagttt
     8161 gttctattat tatattacta ggaccctcaa ctttcaaata ttaacagtaa ttatttttaa
     8221 tttataactc gaaattcata tcttcactta aaaagaaaat attgtcttct ttaaaatcat
     8281 tggtaaaatt aggatctaag accttatttg tatggttaaa gtgagt
//
LOCUS       DATGCJ010000031         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_93221, whole genome shotgun sequence.
ACCESSION   DATGCJ010000031 DATGCJ010000000
VERSION     DATGCJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93221"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..672)
                     /gene="msrB"
                     /locus_tag="VKN64_01340"
     CDS             complement(259..672)
                     /gene="msrB"
                     /locus_tag="VKN64_01340"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMA58904.1"
                     /translation="MSENKEKFPVSKTEEEWKEILTKREFLVLRKGKTDPPYKNEYYH
                     FDEDGIFKCAACGNELFDSEDKYDSGSGWPSFIRPIREVAVDYRDDYKLSHKRTEVIC
                     NQCGSHLGHRFEDGPEPTGYRYCINSTSLEFEERK"
     gene            complement(665..1774)
                     /locus_tag="VKN64_01345"
     CDS             complement(665..1774)
                     /locus_tag="VKN64_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMA58905.1"
                     /translation="MENGILGQTFLNNTIYNYLISLLIIIVGGMAVRLFKYLFLDKIH
                     NMIRKVSESFGGFFNKALDKRVIPLAYLIIIFGSLNRLILSSTIDKALNIIFIFIIVV
                     YITLLIQDIIIYTMKKYWEKKQSNQAQETVFTVSVFIIKLTTWLIALLFILDNLNIEI
                     RGLITGLGVGGIAIAFAAQTILADIFSYFTIFLDRPFDIGDFIIVGDYMGTVEHIGIK
                     TTRIRSLSGEQLVFSNNDLTNSRINNYKRMQRRRINFKFGVIYGTALEKLEKIPTYVE
                     EIINSVEKTTFDRAHFASYGDYSLIYQVVYYVEDSDYKVYMDIQEKINLKLKDKFNEE
                     GIEFAYPTQSVHVYDNADGSDIQPVERIKEEKNNE"
     gene            complement(1803..2246)
                     /gene="mscL"
                     /locus_tag="VKN64_01350"
     CDS             complement(1803..2246)
                     /gene="mscL"
                     /locus_tag="VKN64_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMA58906.1"
                     /translation="MLKGFKKFVLRGNVVEMAVGIIIGLAFNKIINSFVNDILMPPIS
                     LLVGKINFSNLYINLSQREFANMAEAEAAGVPIIKYGVFINTVIDFLLMAFFLYLVIT
                     YYNKLKEMKKEEEIAKAPDTKQCPYCYSTINIKATRCPNCTAELE"
     gene            complement(2264..2617)
                     /locus_tag="VKN64_01355"
     CDS             complement(2264..2617)
                     /locus_tag="VKN64_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HMA58907.1"
                     /translation="MSEEKYLVFAFNGESNCFVHALLNAEEFYQEGFDTKLVIEGSAT
                     KQIKMMDDPDNQFSNLYKRVKDKGLIDCVCRVCAKKMESLDSARKQNLPICDSLSGHV
                     SMARYLKEGYQIITF"
     gene            complement(3095..3622)
                     /gene="hpt"
                     /locus_tag="VKN64_01360"
     CDS             complement(3095..3622)
                     /gene="hpt"
                     /locus_tag="VKN64_01360"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMA58908.1"
                     /translation="MNSKNIGEIVYSEDEIQKKVKEIAANINQDFEDKEPLLIGILKG
                     SIYFLSDLSRLLDINHNIDFLSIGRFQTTEGPREKVKITKDLDLSIRNRDIIVVEDII
                     RTGLTHSYILKNLEPRQPQSINLCVFLDCQEQRLVDLPIKYSGFEMDSRFVIGYGLDY
                     KEKYRNLPFVAEFKE"
     gene            complement(3615..4580)
                     /locus_tag="VKN64_01365"
     CDS             complement(3615..4580)
                     /locus_tag="VKN64_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HMA58909.1"
                     /translation="MFVITISRQTASLGDEIAAELADKLGVKLITRNYVIDNWLSEIA
                     DEHDLHMLRETSKYYLRTAQNGQTFKEYIIERLKKEAEEQSLVILGLGSQVIFKDYAH
                     AVHIKVICSDGRRVKRIMEKYNIQQEQAVRTLDLSDRKHRRYVHRLFNKDWENISLYH
                     MSINTDGLDIKEASNLIIHLLEMKKEEPQPLFRPQEEITVKRMNTKDIDFVHESERDF
                     AEILDMYNIEWEYEPSEFPLEWDAEGNIVLGFRPDFYLPEFDTYIELTTMKQKYVTEK
                     NKKVKLLQELYPDVNVNIVYKKDYHKLLKRFGVNNIREDEDGKDE"
     gene            complement(4689..>5296)
                     /locus_tag="VKN64_01370"
     CDS             complement(4689..>5296)
                     /locus_tag="VKN64_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA58910.1"
                     /translation="FEKGLTSDWGFSCFIESKEEPNVLFDTGTKPNILLDNMKKLKIS
                     PDDIDEVFISHSHHDHTGGLKELLKLNRNIKVYIPASFKFLSDNSTISVKKPIKLHGN
                     YYSTGELNNIEQSLIIKKEEGVTVISGCAHSKVENILNSAFQYGKIRYLIGGLHGFDK
                     YELLEDVDYVCATHCTQNKRQIKKRYPNKWIEGGVGRVINI"
BASE COUNT         1707 a          996 c          688 g         1905 t
ORIGIN      
        1 tatctacagg tttgaattct tttctagtac ttgtaaggtt agtagtttct ccacattcgc
       61 aataaataca gttaaaagaa cagatcttat atggaattag gtcaatacca agtgataacc
      121 ccaaccttct ggagggaaca ggtccatata aatattccat ttttccacct tccttttatt
      181 taatttttaa ggaactgcat ctacactgat gctattatat gatatccgag tattttagtc
      241 aagtttatac tttacaaact atttgcgttc ttcaaactca agtgaagttg aattaataca
      301 gtacctatat ccggttggct cagggccgtc ttcaaaacgg tgtcccaaat gacttccaca
      361 ttgattgcag attacttcgg ttcttttgtg agacagttta tagtcatctc ggtaatcgac
      421 cgcaacctct ctaatcggcc tgataaaact aggccaaccg gaacccgaat catatttatc
      481 ttcagaatca aagagctcgt taccacaggc tgcacattta aatataccat cctcatcaaa
      541 atgataatat tcatttttat agggaggatc tgttttacct tttcttaata ccaaaaattc
      601 tcgcttggtt aaaatctctt tccattcttc ctcagtttta ctaacaggga acttttcttt
      661 attttcactc attatttttt tcctccttta ttctctcaac gggctgaata tcactaccat
      721 ccgcattatc ataaacatgg acactttgag ttggataagc aaattcaata ccttcttcat
      781 taaacttatc ttttaacttt aaatttattt tttcctgaat atccatgtat accttataat
      841 cactatcttc cacataatag acaacctggt aaattaaact gtaatctcca taagaagcaa
      901 aatgtgccct atcaaaggta gttttttcaa cactatttat gatttcttcc acataagttg
      961 gtatcttctc taatttttct agagcagtgc cataaataac tccaaatttg aaattgatcc
     1021 ttcttctctg catccttttg taattattga ttcttgagtt agttagatca ttatttgaaa
     1081 aaaccaactg ttctccactt aagctcctta tcctggtggt tttaatacca atatgttcga
     1141 cagtacccat gtaatctcct acgataataa aatctccgat atcaaaaggt ctatctaaaa
     1201 atatagtaaa ataactgaag atatcggcta aaattgtctg ggctgcaaaa gcaatagcaa
     1261 taccacctac acccaggccg gtaatgaggc ccctaatttc aatatttaag ttatctaaga
     1321 taaatagtag ggcaatcaac caggtagtta gtttaatgat aaagaccgaa actgtaaaga
     1381 ctgtttcctg ggcttgattg ctttgttttt tctcccaata ttttttcatg gtataaataa
     1441 taatatcctg aatcaataag gtaatatata ccacaataat gaaaataaat ataatattta
     1501 aggccttatc aatagtcgaa ctcaaaatta gtctatttaa agatccaaat ataataatta
     1561 aatatgctag tggtataact cttttatcaa gagctttatt aaagaagccc ccaaaacttt
     1621 cagaaacttt tctgatcata ttgtggatct tatctaaaaa taaatattta aataatcgga
     1681 cagccattcc gcccacaatt ataattaaaa gcgatattag ataattatat attgtattat
     1741 ttaaaaaagt ttgtcctaaa attccatttt ccataacaag atcaacccca taattatatt
     1801 aattattcta attcagccgt acagttcgga caccttgtag cttttatatt gatagttgag
     1861 taacaataag gacactgctt tgtatcggga gccttagcta tttcttcttc ttttttcatt
     1921 tctttcaatt tattgtaata agttatcact agatatagga aaaaggccat taatagaaaa
     1981 tcaataacgg tatttataaa taccccatac tttattattg gtactccagc tgcttctgct
     2041 tcagccatat tagcaaattc acgttgactt aagttgatat ataagttgga aaaatttatt
     2101 ttgcccacca gtaaactgat cggcggcatc aatatatcat tgacaaaaga attaattatc
     2161 ttattaaaag caagacctat tataatacca acagccatct ccaccacatt gcctcgcaat
     2221 acaaacttct taaatccttt taacattata atccccctat aatttaaaaa gtaattattt
     2281 gatacccttc ttttagatat ctggccatac taacatggcc ggaaagggaa tcacaaatag
     2341 gcaagttttg tttgcgggcc gagtctaagg attccatttt tttagcacat accctacata
     2401 cacaatcaat taatcctttg tctttaactc ttttatataa attagaaaat tgattatctg
     2461 gatcatccat cattttaatc tgtttggtcg cactaccttc aataactagc tttgtatcaa
     2521 agccttcttg ataaaactcc tcagcgttta ataaggcgtg gacaaaacaa ttgctttcac
     2581 cattaaatgc aaaaaccaaa tatttttctt cagacatctt attacccctt tcgtgtagat
     2641 ttttattaat aataacacgt ttcattaaga aaattcaatc tgtaattata agatatattt
     2701 ataaatatta gatattttgc gatatataga atattacaaa gcattaacaa gctattaaca
     2761 tttaatttac attataggta taatatctgc tttgactaat ttaatatatt cacctataat
     2821 ataaataggc tccaaggaat ccctaagagg atttattcta agtaacaatc tttatcgagg
     2881 ttctagattt aagcgaaatc tggactttaa attggaggtt aatgagagaa atttatagat
     2941 ggttagtact gaataagtac taacatcaat aaataagctc gacattgacc gaggaagatt
     3001 tagagaaagg atttcgagag taaaaaaaga acttcgatgt ataagaaggt tgcgaaagga
     3061 ataaagaatt gagaaaggtt cgttggagcc ttttttattc tttaaactct gccacaaaag
     3121 gtaggtttct atatttttct ttgtaatcca acccataacc tataacaaat ctgctatcca
     3181 tctcaaagcc actatatttt atgggaagat caaccaatct ttgctcctga caatctaaaa
     3241 atacgcagag gttaatactt tggggctgtc tgggctccaa attttttaat atatagctat
     3301 gggttaaacc tgttcttatg atatcttcca caactatgat atctctattt cttatactta
     3361 aatcaagatc cttggtaatc ttcacctttt ctctgggtcc ttcggtagtc tgaaaacgac
     3421 ctatagataa aaaatctata ttatgattta tatctaataa tctacttaaa tcagataaaa
     3481 aatagataga accttttaat ataccgatta ataaaggttc tttgtcctca aaatcttgat
     3541 ttatatttgc agctatttcc tttacctttt tttgaatctc atcttcacta tagacgatct
     3601 cgccaatatt tttactattc atctttacca tcctcatctt ctcttatatt attaacccca
     3661 aaccttttaa gtaatttatg gtagtccttt ttatagacaa tattaacatt aacatcggga
     3721 tataattctt gtagtaattt aactttctta tttttttcag tcacatattt ctgcttcatt
     3781 gtggttaatt caatataggt atcaaattct ggtaaataaa aatcaggtct aaaacctaat
     3841 actatattac cttctgcatc ccattctaat ggaaattcag atggttcgta ttcccactct
     3901 atattataca tatcaagtat ttctgcaaaa tctctttcac tttcatgcac aaaatcaata
     3961 tctttagtat tcatcctttt cactgttatc tcttcctggg ggcgaaacaa aggttgcggc
     4021 tcttcttttt tcatctctaa tagatggatt attaaattag aggcctcttt aatatccagt
     4081 ccatctgtat ttatactcat atggtacaag gaaatatttt cccagtcttt attaaacaac
     4141 cgatgtacat atctcctatg ttttctatca cttagatcaa gtgttctaac cgcctgttcc
     4201 tgctgaatat tgtatttttc cattattctc tttactcttc ggccatcaga acagattact
     4261 ttaatatgga ccgcatgggc ataatcttta aaaataacct gggatccaag tcctaaaata
     4321 acaaggcttt gttcttcagc ctcctttttt aatctttcaa taatatattc tttaaaagtt
     4381 tgcccattct gagcagtcct aagataatat ttcgaagttt ctctcaacat atgtagatca
     4441 tgctcatctg caatttcaga taaccagtta tctatcacat agtttcttgt aattaattta
     4501 actcccaatt tatcagctaa ttcagcagca atttcatcac ccagactggc agtttgtctt
     4561 gatatggtta ttacaaacaa tttccttccc ccccttaccc tactacatat ataatttctt
     4621 gatatttatt aatattcctc tataaaataa aaaagaccct gagagggcct taatgaatta
     4681 tatattattt aaatattaat aaccctacct accccacctt caatccattt attaggatat
     4741 ctttttttaa tctgtctttt attttgggtg cagtgggtag cacagacata atcgacatct
     4801 tccagcaatt cgtatttatc aaaaccatgg agaccgccga ttaaatatct aatctttcca
     4861 tactggaaag cactattcaa aatattttct accttagaat gggcacagcc agaaatcact
     4921 gtaactccct cttctttttt gataataaga ctctgttcta tgttatttaa ttcacccgta
     4981 ctgtaataat taccatgtag tttgatcggt ttttttacag agatggtact gttatcactt
     5041 aaaaatttga aggaggcggg tatgtaaact ttaatatttc tattcaattt gagtaattct
     5101 tttaatcctc cggtatggtc atgatgagag tgagaaataa atacttcatc gatatcatca
     5161 ggacttatct ttagtttctt catattatcg agtaaaatat ttggcttggt acccgtatca
     5221 aacaatacgt tgggttcttc cttgctttct ataaagcagg aaaatcccca atctgaggtt
     5281 aatccttttt caaaaa
//
LOCUS       DATGCJ010000032         7401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_316617, whole genome shotgun sequence.
ACCESSION   DATGCJ010000032 DATGCJ010000000
VERSION     DATGCJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316617"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /gene="smc"
                     /locus_tag="VKN64_01375"
     CDS             <1..2154
                     /gene="smc"
                     /locus_tag="VKN64_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMA58911.1"
                     /translation="QARYDKLESKYEEIQDELYQKKSRREFIEDLKKEYSGFYKGVKN
                     ILKEKDRFPQMYGAVAELINVSKKYETAIETTLGSKLQNVVVENDKTAQQCIEYLKEN
                     KSGRATFLPIQSLKTYGRINTTQFEQKTGYLGLALDFVSFKDKFQKVMEYLLASTIIS
                     KDLDSAIEISNIINKKYKLVTLEGDVIYPGGSVSGGSRQSKKNNLLGREREVNELEDE
                     IAELDEQIDEIVKEGLDIKDKIEKLEDNRDELKDKYQKLKLKLENLKENEEELKDQLA
                     EVKDEVNTIQDKIEEGVNELTSLKDKKSNLSGVFDKNSQNYDEEKDIINQKQEKIDKL
                     NDDIENLREKLTDVRVKFATQQEKLKSLSKDINNLEDSIEQNKNNQENKETRIEEIKK
                     EVNDLAERKVKLLDQKEEFENEVEEAAQKLEEMEQNLKQMRAEHEKLENEVEELNDEL
                     NEKKDKLHEINIDYTRKKDKSESIEERLINEYNFDINHDQYEEREKVENRQQVEQRVK
                     ELREQISKLGSVNIGAIEEYEELKERLDYLHEQQSDLEEAKESIINIIERIDETMMDK
                     FKTTFYQIKEEFEEVFVELFNGGRAELNLVDEEALLDTGVEIIAQPPGKKLKKLTLLS
                     GGERALTAIALVFALLRVNPSPFYILDEIDAPLDDVNVVRFSDFLKEYTDFAQFIVVT
                     HRKHMMTAVDNIYGLTMTDKGVTELVSLNLKEQIQ"
     gene            2180..3130
                     /gene="ftsY"
                     /locus_tag="VKN64_01380"
     CDS             2180..3130
                     /gene="ftsY"
                     /locus_tag="VKN64_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMA58912.1"
                     /translation="MSIFKNAENNEEEDKGLFKKLKDGLNKTKKGIFGKLNDLFTGGK
                     ELDDEFFTELEEVLIQADIGVHTTMKMIDDLKTKVEKEDIEDTTEFYGVFKEELSEFL
                     QDEEGGIKLEDGLNIIMVVGVNGSGKTTTIAKIASKFKKQGKDVMIAACDTFRAAAID
                     QLEVWTNRLGVRLIAQKEGSDAAAVAYDAVQSARSKEADVLIVDTAGRLHTQKNLMEE
                     LKKVKRVIYKEAEEAHKQILLVLDATNGQNAISQAKLFHEAVNIDGIALTKLDGTAKG
                     GVVIAVKDELGIPIKLIGVGEKEDDLQVFKPDKFVDALFS"
     gene            3223..3567
                     /locus_tag="VKN64_01385"
     CDS             3223..3567
                     /locus_tag="VKN64_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HMA58913.1"
                     /translation="MLDKTIEISMLFDYYGKLLTDKQQKIIDLYYYQDLSLGEIAENL
                     DISRQGVYDHLNRAENTLKSYEDKLQLVKRNKKNIGIINELIRHIKDNDEIENKTKNY
                     LLDKLKEMNKNI"
     gene            3587..4933
                     /gene="ffh"
                     /locus_tag="VKN64_01390"
     CDS             3587..4933
                     /gene="ffh"
                     /locus_tag="VKN64_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMA58914.1"
                     /translation="MIFSGLAEKLQDTLKKLSGKGKLTEKDVDSALREVKLALLEADV
                     NYKVVKNLINNIKERAIGQEVMDSLTPGQQVVKIVNEELTKLMGGEQSEIEISSEPPT
                     LIMLVGLQGSGKTTTAGKLAKMFKKEGKNPLLVAGDIYRPAAIRQLQVLGDRLEIPVF
                     SMGDKQDPVNIIKAGLSKAESAGNDIVILDTAGRLHIDEEMMDELQDIKTHTDPDEIL
                     LVVDSMTGQDAVNVSENFDEKLGIDGVVLTKLDGDARGGAALSVKAVTGKPIKFIGTG
                     EKLEDFEPFHPDRMSNRILGMGDVLSLIEKAEEAIDQEKAKKLEERLKNNQFTFEDFL
                     DQLEQLRDMGPLDQIMGMIPGMSGAKQLKNLQVDEKQLDHIEAIINSMTQEEKVEPDI
                     INGSRRKRIAQGSGTSIQEVNRLLKQYRETKKMIKKFNSGKGKGLMGKMGNMNLPF"
     gene            4986..5252
                     /gene="rpsP"
                     /locus_tag="VKN64_01395"
     CDS             4986..5252
                     /gene="rpsP"
                     /locus_tag="VKN64_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMA58915.1"
                     /translation="MAVKIRLKRMGKKRSASYRLVVSDSRNAPIGKFLEELGYYDPTT
                     EPETYKIDEEKALKWLNNGAKPSNTVKSLLKKSGIYEKFLNQEK"
     gene            5268..5495
                     /locus_tag="VKN64_01400"
     CDS             5268..5495
                     /locus_tag="VKN64_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HMA58916.1"
                     /translation="MEELVEVIARNIVDQPEKVTTNKIEDGNTTVVELSVADDDMGKV
                     IGKQGRIARAIRKVVKASATKEGKKVEVKIK"
     gene            5512..6027
                     /gene="rimM"
                     /locus_tag="VKN64_01405"
     CDS             5512..6027
                     /gene="rimM"
                     /locus_tag="VKN64_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405874.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMA58917.1"
                     /translation="MEKLISLGKITKNQGNKGEVRVAPYTDDLRRFEFLSEVILVSPD
                     QTEKITKTVQDIWLHKNFVILKLKGIDDIGEALEYRNYEVKIAEEELLPLAEDEFYVD
                     DLIDSTVILPDERILGKVLDVIDTGGTDILIIKGDTKEYMIPMSKEYIKKIDIKANKI
                     FIDPVNGILNL"
     gene            6045..6800
                     /gene="trmD"
                     /locus_tag="VKN64_01410"
     CDS             6045..6800
                     /gene="trmD"
                     /locus_tag="VKN64_01410"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMA58918.1"
                     /translation="MRFDILTLFPEMFNGPFSDSMIKKARENGIIDINLIDIRDYTTD
                     KHNTADDYPYGGGAGMVLKVEPIYYALADITEDFSQSTTNILLTPRGQKLDQSMVKDL
                     SGKDRLVLICGHYEGIDERIRNNFVDQEVSIGDYVLTGGEIPAMVLVDAVARLLPGVL
                     GHDESKKNDSFYNGILDYPHYTRPQEFKGMEVPEILLSGNHQLVDRWRKKEALKRTYL
                     MRPDLIENTELTQDQQELLREIKQDIEGDSNNE"
     gene            6793..7350
                     /locus_tag="VKN64_01415"
     CDS             6793..7350
                     /locus_tag="VKN64_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMA58919.1"
                     /translation="MNKNVFIALLHHPVYNKDGEVITTTVTNYDLHDISRAARTYGIN
                     KYYVVNQLKSQQSLVKRMRDYWTSGFGADYNGTRHEAFKVMEIADTLADTIEDIKNHT
                     GEKPTLITTSAQKSKETISFTDLKEKMHSTDQPYLIIFGTGWGLTKEIIEKSDYLLEP
                     VTGNTDYNHLSVRSAASIIMDRLLS"
BASE COUNT         2815 a         1062 c         1604 g         1920 t
ORIGIN      
        1 caagccagat atgacaaatt agaaagcaaa tatgaagaaa ttcaggatga actctatcaa
       61 aagaaatccc gtcgggaatt tattgaagac ctcaaaaagg aatactccgg tttctataaa
      121 ggtgttaaaa atatccttaa agaaaaagac cgttttcccc agatgtatgg ggcggtggct
      181 gaactaataa atgtaagtaa aaagtatgaa accgcgattg agactaccct cggtagtaaa
      241 ctgcagaatg tagtagtaga aaatgacaag acagcccaac aatgtataga atatctaaaa
      301 gaaaacaaga gcggccgagc cacattttta cctattcaat ccttaaaaac ttacggccgg
      361 attaatacca ctcagtttga gcaaaaaact ggatatttag gtttagccct tgattttgtt
      421 agttttaaag acaagtttca aaaagttatg gagtatcttt tagccagtac cataatttct
      481 aaagacctcg attctgccat tgaaatctca aatataatta ataaaaaata taaattggtc
      541 actttagagg gagatgtaat ttaccccggt ggttctgtat caggcggcag tcgacagtct
      601 aagaaaaaca acctattagg tcgtgaaagg gaagttaatg aattagagga tgaaatcgcg
      661 gaattagatg aacagattga tgagatagta aaagaaggtt tagatataaa agataagata
      721 gaaaaattag aagataatag agatgaattg aaggataagt accaaaagtt aaagttaaaa
      781 ttagaaaatc taaaagagaa tgaagaggaa ttaaaagatc agttagccga ggtaaaagat
      841 gaagttaaca cgattcaaga taaaattgaa gagggtgtaa atgaacttac ctcactcaaa
      901 gataagaagt ccaatttaag cggtgttttt gataaaaaca gccaaaatta tgatgaagag
      961 aaggatatta taaatcaaaa acaggaaaag atcgataagc taaatgatga tattgaaaac
     1021 ttaagagaaa aactaacaga tgttagggta aaatttgcaa cccagcagga aaaactaaaa
     1081 agtttaagca aagatatcaa taatttagag gattctattg agcagaataa aaataatcag
     1141 gaaaataaag aaacaagaat agaagaaatt aaaaaagagg ttaatgatct agctgaacgc
     1201 aaagttaaac tcttagatca aaaagaagag ttcgaaaatg aggtagaaga agcggctcag
     1261 aaattagaag agatggaaca gaatttaaaa cagatgaggg cggaacatga aaaattagaa
     1321 aacgaagtag aagagctcaa tgatgagtta aatgaaaaga aagataaact gcatgagatt
     1381 aatattgact ataccaggaa aaaggataag tcagaaagta ttgaagaaag gttaattaac
     1441 gaatataact ttgatattaa tcatgatcaa tatgaagaaa gagaaaaagt cgagaacagg
     1501 caacaggtag aacagagagt aaaggagtta agagagcaaa tttctaaatt gggaagtgtc
     1561 aacatcggag caattgaaga atatgaagaa ttaaaagaaa gattggacta tctacacgaa
     1621 caacagtccg atttagaaga agccaaagag tctattataa atataatcga acgtattgat
     1681 gaaacgatga tggataaatt taaaactact ttttaccaga ttaaggaaga atttgaagag
     1741 gtttttgtgg agttatttaa cgggggaagg gccgaactta atttagtaga tgaagaagcc
     1801 ttacttgata ccggggtaga aataatcgcc caaccacccg gtaagaaatt aaaaaaatta
     1861 actctgttat cagggggaga aagggccctg actgcgatcg ctttagtatt tgcattacta
     1921 cgcgtaaatc ccagcccctt ttatatccta gatgaaattg atgccccctt agatgatgtc
     1981 aatgtagtgc gtttttctga tttcttaaaa gagtatactg attttgccca atttattgta
     2041 gtaactcacc gcaaacacat gatgacagcg gttgataata tttatggttt aacgatgacc
     2101 gataaaggag taactgaatt agtatcctta aacttaaaag aacaaattca ataaggaata
     2161 gggaggagta taattaacga tgtctatatt taaaaatgca gaaaataatg aagaggaaga
     2221 taaaggactc tttaaaaaat taaaggatgg tttaaataaa acgaaaaaag gtatcttcgg
     2281 caaattaaat gacctcttta cagggggaaa agaacttgat gatgaattct ttaccgaatt
     2341 ggaagaggtt ttaattcagg cggatatcgg tgtccacacc acaatgaaaa tgatcgatga
     2401 cctaaaaaca aaggttgaaa aagaagatat agaagataca accgagtttt acggggtttt
     2461 caaagaagag ttaagtgagt ttttacagga tgaagaaggt ggtataaaat tagaagacgg
     2521 tttgaatata ataatggtag tcggggttaa cggttctgga aaaaccacta cgattgccaa
     2581 gattgccagc aagtttaaaa aacagggtaa agatgtcatg attgcagcct gtgatacctt
     2641 tagagcagct gcaatagacc aattagaagt ttggacaaat aggctaggtg ttaggcttat
     2701 tgcacaaaaa gaaggctcag atgctgctgc agtggcttat gacgccgttc aatcagctag
     2761 atctaaagaa gccgatgtac taattgtgga tacagccggc cgcctccata ctcaaaagaa
     2821 cctgatggaa gagttaaaaa aggttaagag agttatctat aaagaagcag aggaagccca
     2881 taaacagatc cttttggtat tagatgcaac caatggacag aatgccatct ctcaggccaa
     2941 attattccat gaagctgtca atatagatgg tattgccctt accaagctgg atgggacagc
     3001 taaaggtggg gtagtaattg ctgttaaaga cgaactgggg atcccgatta aattaatagg
     3061 tgtgggagaa aaggaagatg acctacaggt ctttaaaccc gataagttcg ttgacgcgct
     3121 attttcttga aaactaaccc ttgacaacct gctttttata agttataata tgctctgtga
     3181 attggtgtaa agtaataata cttaacaagc ttggtgatta aaatgctgga taaaacgata
     3241 gaaattagca tgttatttga ctactacgga aagttattga ccgataagca gcagaaaatt
     3301 attgatctct attattacca ggatttatca ctcggcgaaa ttgcagaaaa tctagatatc
     3361 agcagacagg gtgtttatga ccatttaaat cgagcagaaa acacactcaa gtcttatgaa
     3421 gataagttgc agttagtaaa aagaaacaag aagaatatcg ggataataaa tgaactaatc
     3481 aggcatataa aagataatga cgagattgaa aataaaacga agaactattt attggacaag
     3541 ttaaaagaaa tgaacaaaaa tatttaagct ggaggtacag ttttaaatga tattttcagg
     3601 tttagctgaa aaactgcagg atacattaaa aaagttaagc ggtaaaggaa aattaactga
     3661 aaaggatgtt gactctgccc taagagaggt taagttagct cttctcgaag cagatgttaa
     3721 ctacaaagtt gttaaaaact taataaacaa tataaaagag agagctatcg gccaggaagt
     3781 aatggatagt ttaacacccg gccagcaggt agtaaaaatc gttaatgaag aacttaccaa
     3841 attaatgggt ggtgaacaga gtgaaattga aatttcatct gaacccccta ctttaattat
     3901 gctggtaggt ttacagggtt ctggtaaaac aaccacggct ggtaaactgg ccaagatgtt
     3961 taaaaaagaa gggaaaaatc ccctcctcgt ggcgggagat atctaccgtc ccgcagcgat
     4021 tagacagctg caggttttag gggatagact ggagattccc gtcttttcaa tgggtgataa
     4081 acaagatccg gttaatatca ttaaggcagg tttaagtaag gccgaatctg ccggtaatga
     4141 tatagttatc ctggatacgg ccggccgatt acacattgat gaagagatga tggatgaact
     4201 acaggatatt aaaactcata ctgaccccga cgaaattctt ttagttgtgg attccatgac
     4261 tggtcaggat gctgttaatg tatccgagaa ctttgatgaa aaactcggta tcgatggcgt
     4321 tgtattaacc aagttagatg gtgatgcccg gggtggggcc gctttatctg ttaaagcagt
     4381 aaccggtaaa ccgattaaat ttatcgggac cggtgaaaag ttagaggact ttgaaccctt
     4441 ccatcctgat aggatgtcta ataggatatt gggaatgggt gatgtgctaa gccttatcga
     4501 aaaggcagaa gaagccatcg accaggaaaa agccaagaaa ttagaagaaa gattaaaaaa
     4561 taatcagttt acctttgaag acttcttaga tcagctagaa cagctgagag atatgggacc
     4621 acttgatcag attatgggca tgattcccgg aatgagtggg gccaaacaac tcaaaaattt
     4681 acaggtagat gaaaaacaat tagaccatat tgaagcaata attaattcga tgactcaaga
     4741 agagaaggtt gagcctgaca taattaacgg cagccgccgc aaaagaattg cccagggaag
     4801 cggtaccagt attcaggaag taaacaggct attaaaacag tatagagaaa ctaaaaagat
     4861 gatcaagaag tttaacagcg gtaaaggcaa aggtttaatg ggtaaaatgg gtaatatgaa
     4921 cctacccttt taacctttat attataaaag tagacactgt aaatatttca aggaggtgaa
     4981 tgtcaatggc agtaaagatt agactaaaga gaatgggtaa aaaaaggagt gcatcatata
     5041 gattagtggt ttctgattcc cgtaatgctc cgattggaaa gtttctggag gaattaggat
     5101 attatgatcc taccaccgaa cccgaaactt ataaaattga tgaggaaaaa gctcttaaat
     5161 ggttaaacaa tggtgctaag ccatccaata ctgttaagag tttattaaag aaatcaggta
     5221 tttatgagaa gtttttaaat caggaaaagt aaaaggaggt tgctcctgtg gaagaattag
     5281 ttgaagttat tgcaagaaat attgtagatc aacccgaaaa agtaacaacc aataagattg
     5341 aagacggcaa tacaactgta gtagaactct ctgtagctga tgacgatatg ggtaaagtga
     5401 tcggtaaaca gggtagaatt gcccgagcaa tccgtaaggt tgtcaaggct tctgctacaa
     5461 aagaaggtaa aaaagttgag gtcaaaataa aatagtaatg gtggtataaa aatggaaaag
     5521 ttaatatcac tgggtaaaat aacaaaaaat cagggtaaca aaggggaagt aagagtagcc
     5581 ccttatactg atgatttaag gagattcgag ttcctgtcag aagtaatact ggtaagccct
     5641 gatcaaactg aaaagataac caagactgtt caagatatct ggcttcataa aaattttgtt
     5701 attcttaagc taaaaggcat agatgatatt ggtgaagctt tagagtatag aaattatgag
     5761 gtaaagatcg cagaagagga acttttacct ctggcggaag atgagtttta tgtggacgat
     5821 ttgatcgatt ccacagtgat cttaccggat gaaagaattc ttggtaaggt tttagatgta
     5881 attgataccg gtggtacaga tatcctaatt ataaaaggtg atacaaaaga atacatgatt
     5941 ccaatgagta aagaatatat aaaaaagata gatattaaag cgaataagat atttatcgat
     6001 ccagttaacg ggatcctaaa cttataggct ttggggtgta ataaatgaga tttgatattt
     6061 taacattgtt tcctgaaatg tttaatggcc ccttttcaga tagtatgatc aagaaagccc
     6121 gtgaaaacgg tattatagat ataaacttaa ttgatattcg agattatact actgataagc
     6181 acaatactgc cgatgattat ccttacggtg gtggggccgg tatggttctg aaagtagaac
     6241 cgatctatta tgcactagcc gatatcacag aagatttcag tcaatcaacc actaatatac
     6301 tattaacccc acgcggccaa aagttagatc agtccatggt aaaagattta agtggaaaag
     6361 accgcttggt cttaatctgt ggacactatg aaggtattga tgaacgtatc cgtaataact
     6421 ttgtggacca ggaagtttcc ataggtgatt atgtgctgac cgggggagaa attccggcca
     6481 tggttttagt agatgctgtt gccaggctgc tgcccggagt attaggtcat gatgaatcta
     6541 aaaagaatga ttctttttat aatggaatcc tggactatcc tcattatacg aggccgcaag
     6601 agtttaaagg aatggaagtg cccgaaatac tgttaagcgg taaccatcaa ttagttgata
     6661 ggtggagaaa aaaagaagcc ttaaaaagaa cctatttaat gagacctgac ttaattgaaa
     6721 atactgagtt aacacaagac caacaggaac tgctgcgtga aatcaaacaa gatattgagg
     6781 gagattcaaa taatgaataa aaatgttttt attgcactac tacatcatcc tgtctataat
     6841 aaagacgggg aagtaataac cacaacagtt acgaactatg atttacatga tatttccaga
     6901 gcagccagga cctatggtat aaataaatat tatgtagtta atcagttaaa gtcccagcag
     6961 tctttagtaa aaaggatgag ggattactgg actagcggtt ttggggctga ttacaatggt
     7021 acaagacacg aagcctttaa ggtaatggaa atagccgata ccttagccga tactattgaa
     7081 gacataaaaa atcacacagg tgaaaaacca accctcatta caaccagtgc ccaaaagagt
     7141 aaagagacta tatcttttac agatttaaaa gagaagatgc atagtacaga ccagccgtat
     7201 ctgataatat ttgggaccgg ttggggatta acaaaagaaa ttattgaaaa atctgattac
     7261 cttttagaac cggttacagg taatacagat tataatcatc tttcggtcag aagcgctgct
     7321 tctataatta tggataggct tctgtcctaa aaagtatatt ccaatatatg ttactgaaag
     7381 gaggtagaca aatgaatatt a
//
LOCUS       DATGCJ010000033        28223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_450340, whole genome shotgun sequence.
ACCESSION   DATGCJ010000033 DATGCJ010000000
VERSION     DATGCJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 28223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28223
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450340"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2665
                     /locus_tag="VKN64_01420"
     CDS             <1..2665
                     /locus_tag="VKN64_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HMA58920.1"
                     /translation="AEKRLEEYGENKLVRLKKTPEIIKFLLQFRDVLMIILLVAAGMS
                     FIIQNYRDGLVMLVIALINAVIGYFQEHKAEAIMESLEQLIQSPSKVIREGQTKEISQ
                     NKLVVGDIVVLEEGDKVPADLRIIESFNLKTNDVSLTGESLPQGKNSNKMEEELSLAD
                     RENMAYLGTNVASGSAKGVVVATGMDTEMGKIANMTQEEEKSKSPLQMELQSVANRIA
                     VFAVIIGTLLFGASIYQGLGFEFAIIYGLGIAVAVVPQALPMQITVALAQGVDRLAKK
                     NAVVKKLSSVETLGSTNVISTDKTGTLTKNEMTVQKVYYDGQEYDVTGIGYEPRGKIL
                     DQDGNEVSQEIIDHLEIMFDAATMASNAEIHPPDDEHSSWYPIGDPTEAALITLSTKV
                     GTRAENEDEENPELHEFSFDSERKRMSSVRQFEDGEYLAMKGALDSVLSVSKYIYKDG
                     KKVPITEEDKEKYRKLNQKYSENAMRVLAFAFRKLGPEHKDYVLEEVEKDVIFLGIVA
                     MIDPPKEGVRESIEQAHAAKINTYIMTGDHSTTAKAIGKEINLVKDEENIEVVTGKEF
                     DNLSDEDLKTMMDENESLIFSRVSPEHKLRIVKNLKDQQKIVAVTGDGVNDAPALKSA
                     HIGVAMGGMGTDVSKQAAELILLDDSFPTLVYAIKEGRNIYNNLKKTVIASLTSNGGE
                     LAVVLLGLLGVSLFNLPVPILAIQILAIDLLAEILPLTAFTFDPPSKDIMKSPPRDKD
                     EHIVNKRSFSEIAFFGLLMGFLAFLNYYLFSSRMGIMDMTEAHPLYARATTITYLTIA
                     FTQLTNIMSRRYEKVTFFNKNILSNKKMVYSMILSILMIVTAIYTPFINQYLGFAGIT
                     FVDWLYVLGSAGIFLGAHELLKVYKRSSENY"
     gene            2683..2805
                     /locus_tag="VKN64_01425"
     CDS             2683..2805
                     /locus_tag="VKN64_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HMA58921.1"
                     /translation="MNVKKKGEVYKCGVCGNVVEVKEVGGGELVCCGQPMNLQK"
     gene            2863..3411
                     /locus_tag="VKN64_01430"
     CDS             2863..3411
                     /locus_tag="VKN64_01430"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HMA58922.1"
                     /translation="MEKFKVNQTQVVLKQGDITEEACDGIVNAANSSLMGGGGVDGAI
                     HRAGGPEILEECKEIRERQGKLPTGKAVVTTAGKIKAEGVIHTVGPIWNGGDSKEEEL
                     LANAYRNSLIVAEENSYQTVCFPSISTGAYGFPLARAAEIALNTIKDYIQNNDHFKQV
                     RMILFSEEDYNIYLKIAKDIFK"
     gene            3450..3833
                     /locus_tag="VKN64_01435"
     CDS             3450..3833
                     /locus_tag="VKN64_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMA58923.1"
                     /translation="MSVKQDTLEMNNWAVVGATDKVEKYGYKIVKTLDEHGYKVFPIN
                     PSLDEIDGIKVYDKLADIEEPVDVVDMVVNPQIGKHVMKEIKKLGIKNVWLQPGTRSD
                     EIREFAAENDINVVESCIYATLKSR"
     gene            3866..5362
                     /gene="glpK"
                     /locus_tag="VKN64_01440"
     CDS             3866..5362
                     /gene="glpK"
                     /locus_tag="VKN64_01440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMA58924.1"
                     /translation="MSKYILAIDQGTTSTRALLYDKNGNIVEKAQKEFTQYYPKPGWV
                     EHDPEEIWNSTIGVINNVIKKSEIKPYDIDSIGITNQRETTVVWDKNTGEPLYNAIVW
                     QCRRTSDICKNLKEEGYEELFRDKTGLLLDPYFSGTKIKWILDNVEGARKKAEENKIL
                     FGTIDTYLIWKLTDGKVHVTDYTNASRTLLYNIHDLKWDQELLDILDVPSYILPDVKS
                     SSEIYGYTDKNVFFGQHIPIAGIAGDQQAATFAQGCYEEGMTKITFGTGAFLLMNTGN
                     EPVYSENGLLTTIAWGCDGEITYALEGSIFNAGSAIQWLRDELFLLNEAPDSEYFAGK
                     VESSDGVYFVPAFTGLGAPYWNPEVRGTIVGLTRGSSKNHIIRATLEALVYQSKDLIQ
                     AMLEDSGLKLKEVKVDGGASANNLLMQFLADMTNTKVERPVNTETTVTGAAYLAGLAT
                     NFWNDLDDLMKKRKVEKVFTPKMEETERSERYEGWLKAIDTALYWSEE"
     gene            5612..6268
                     /locus_tag="VKN64_01445"
     CDS             5612..6268
                     /locus_tag="VKN64_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMA58925.1"
                     /translation="MLKNLFSNHAQELQNDINKYLEVVTNSNMVFKRSCKNYLKDNIE
                     SFEDSLDEINKLENSADDFQKDIKYKLYKYMLIPEARGDVLALLENIDNIVDHIKKLL
                     VQFSIERPTIPDSLNEDVSELVDMVSDSVDVLIKCIRAYFKNISMVNEYANKVHFYEH
                     EADKIEESVKRKVFEDSNLEYFSQKTHLRYFVEQITEISDRAETISERASVAAIKRRI
                     "
     gene            6373..7170
                     /locus_tag="VKN64_01450"
     CDS             6373..7170
                     /locus_tag="VKN64_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HMA58926.1"
                     /translation="MDKIKDTKLFIFDMDGTIYLGDNLIEGVLDFIAKLKKENKQYIF
                     LTNNSSKNSKDYKEKLNKLGIQATTDDIVNAGEVTADYLKGIKANGEDNVYVVGTPSL
                     EKVFEDYGFNLIKDRDKNVDFLVIGFDTTLTYKKLWDAHYLINQGVKYYATNPDKVCP
                     LEDGRSMPDCGSIINLLKTSTGQEPVVVGKPSKLMVNYISNKYSIDKDKISMVGDRLY
                     TDIKMANQSGINSILVLSGETKQEDLKCAQIQPDFVLDSVVEIGNMF"
     gene            7184..7915
                     /locus_tag="VKN64_01455"
     CDS             7184..7915
                     /locus_tag="VKN64_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HMA58927.1"
                     /translation="METKIIAHRGSSNKAPENTEVSFRQAVNDKADGVEFDVHLTADK
                     EVVVIHDEKIDRTSNQSGYVKDYTLKELKRFDFGSYFAKRFKDQSILSLEETLDIVQN
                     MELINIEIKKGYGINQGIEKKIVEIIKGKGLLDKVLISSFNHDSLKIIKDIDSKIKTA
                     ALLFARLHKPWQYAKNLGCDYLHLYYKLLDKEIIDSCHKKNVKVNTFTVNDMADMQKL
                     IYINVDGIITDYPMEALKLLEEVNS"
     gene            7931..9430
                     /locus_tag="VKN64_01460"
     CDS             7931..9430
                     /locus_tag="VKN64_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA58928.1"
                     /translation="MQIIVIGAGVIGSAIARELARYDVEILLIEKEPDVADGTSKANS
                     GIIHAGYNADFDTLKGVMNVKSNPSFDKLCEDLKVPFDRNGSLVVGFDDDDLKKLKEK
                     KANGIKNGIEGLKIIEREEILEKEPNINKEAKYALYAPTAGIISPYELTIGYADNAVR
                     NGAEVLLDTQVTDLIIEDDEVKGVLTNRGEFRADLVINAAGLYSDRIAKMAGDQFEIH
                     PRKGEYHLFDKVHGDIVNHTLFPMPTETSKGILVLPTVHGNLLIGPNANVIDDKDDFS
                     TTSDGLEEVFKGAKKLVPSLPESGVITSFSGLRAALPSEDFHIGFSENKKALINLIGI
                     QSPGLSSAPAIADKVVEMAEQFSKNNNIEFNKKDDFKEANPRYPHYHHYEENDKVEEW
                     QEIVKQDEKYGEIICRCEHVSRGEIIDAINRPVPARTVDAIKRRTRASSGRCQAGFCG
                     PRILDILSNHLKIDPLEVTKKGKGSKILKAKSKEIILKNLEEDSKVGDQ"
     gene            9431..10708
                     /locus_tag="VKN64_01465"
     CDS             9431..10708
                     /locus_tag="VKN64_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA58929.1"
                     /translation="MQEYNMVVIGAGPAGMAAALEAEKNGLTDIVILERDEYIGGILP
                     QCIHNGFGLEYFGEELTGPEYAYEFIDELADSNIEVLTETMVLDVDENKEVTALNKEK
                     GVLKLKGKTIVLAMGCRERTRENIRIPGDRPSGILSAGTAQRYTNIEGYMPGEKVVIL
                     GSGDIGLIMARRMRLEGADVKAVLEIMPFSSGLVRNKVQCLDDFDIPLKLQHTITRIE
                     GQERLERVRVAQVDEDFNPIEGTQEWIECDTLLLSVGLIPENELSKAMGLDLHPVTGG
                     TFVNENRETSIEGVYAAGNVLHVHDLVDWVSEEAEIAGKAAADYINNNLEKRAQKIEI
                     TPAENVTYTVPQTIEYTFGDKKFIKVFMRVTEPMEDVEVKFSANKEEIISYPKKIVMP
                     GEMVTANLPAALVTDEMEEIKVSIVKKAGENNG"
     gene            10701..11075
                     /locus_tag="VKN64_01470"
     CDS             10701..11075
                     /locus_tag="VKN64_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HMA58930.1"
                     /translation="MAKNDIICTGCPKGCHVTVEVEDDEIVNVEGYECPTGREYAINE
                     YKNPTRILPSTIRVKKGEFPLVPVKTAQPIPKSLLIKAMDVIAKTEVKAPIKIGDVLI
                     ENILDTGVDIVATRNIDKEVEV"
     gene            11079..11645
                     /locus_tag="VKN64_01475"
     CDS             11079..11645
                     /locus_tag="VKN64_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HMA58931.1"
                     /translation="MKESLEEYFADFKVIAGISDDSRLEKALFSKVLGVFLLNAKLNK
                     LNEYAENVKEHNKKLFLHLDLMKGMNSDKEAVHYLASNDLCDGIITTHKNLIKYAKKE
                     GLYTVQRIFVLDSGTIKNGIKSLKTVRPDAVEILPGLMAPHFINELSMELDIPFIAGG
                     LIRTREEVNKLLKDGVFAVSTSEDELWY"
     gene            11791..13182
                     /gene="asnS"
                     /locus_tag="VKN64_01480"
     CDS             11791..13182
                     /gene="asnS"
                     /locus_tag="VKN64_01480"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMA58932.1"
                     /translation="MDYFKKSKISNILQGKIDSYEDVIVLGWVKTKRVSKNIAFLEVN
                     DGSTVKNLQIVVLEPSNFELEDINRGSSVRIKGYLEDVPNREQSIEMKAEEIDIIGTA
                     PENYVLQKKEHTFEFLREIAHLRPRTKTFSTVNRFRNKMAYGIHKYYQERGFQYIHAP
                     IITASDAEGAGEMFKVTTLDLDNITKDDKNKVNFTEDFFGEQTGLTVSGQLEAEVMAT
                     ALGDVYTFGPTFRAENSNTSRHASEFWMMEPEMAFCNLEEMMDIIEDFIKNLFEYALN
                     EAAEEMEFFAENIEEGRKETLKNIINSTFARITYTEAIDILKEADQDFEFPVEWGMDL
                     QTEHERYLTEQHYQKPVLVTDYPKEIKAFYMRLNDDQKTVAAVDCLVPEVGEIIGGSQ
                     REERYDVLKERMEEENMNIDKYDWFLELRKFGTVPHSGFGLGFERILMYISGIKNIRD
                     VIPFPRTPGNAKF"
     gene            13169..15019
                     /locus_tag="VKN64_01485"
     CDS             13169..15019
                     /locus_tag="VKN64_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA58933.1"
                     /translation="MLSSKYRFLISLVIILLLTTPILSAQSNLTLDYDVVVYSGQPGG
                     VTAAVSAARSGLSTLLILKRDNPGGLMTYGGLNYLDLNFDKAGKILNKGLFAEWYERI
                     GKNQSFTYDKATQVFNEMLNEAGVVVIKNSNLERVCLKDNNIEKMLIKDEKENYFQIT
                     ADYFIDAQQDAQLAFKAGANLFNFGSDINLNDRFMAMTLVLNIDDININNLKKDIVSN
                     RYGPSYLKNGHAYGFKELGKIYEPHNENLKLRGLNIIFNENHNGYHQGSINALLIFNH
                     NPLDQKEREAAYQLAAEEAKYILEFLKEKLNGFKKAKLNEPAEELYIRESRHLVAENI
                     LQVEDLLENKISDNTITLASYPLDYQASEMDYQGFVLFNPVVYGIPFDSLLPDNIRNL
                     MVVSRSTGLSSRAASSARVIPARMNSGAAAGKAAALADENEISLKELNKNKKLIAEIQ
                     QYMEINISEYKDIKPLFKKDNTYPYIKELFNLGLIIGGYQNEFGFDSTMSEYEFGQLL
                     IKGMIRRNSPIFYEWVPGSLETLTTLKKLDKLNAYKLILAASSHQVLEIAEEKYLDYL
                     REDNLIPTDFEESVKSNRVLNRKEAYYLIGYFLKQSPLPNKLKILRGTKK"
     gene            15016..15489
                     /locus_tag="VKN64_01490"
     CDS             15016..15489
                     /locus_tag="VKN64_01490"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMA58934.1"
                     /translation="MSKEVKYLKSPLGLLKITVKHDKLISIKYVKEKGKQLVKEINSE
                     VMKKTLIQLEEYFNHKRNNFELPLSIEGTDFQKDVYKALLKVNYGGVISYKKLAVNSG
                     HPKAYRAVGTALKKNKIPIVIPCHRVIKSDHSLGNYTGGKWRKEELLELEESEFI"
     gene            complement(15475..16257)
                     /locus_tag="VKN64_01495"
     CDS             complement(15475..16257)
                     /locus_tag="VKN64_01495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMA58935.1"
                     /translation="MYKLPDINLEVNPLAEEVRLKGSKKDLVILIHGYTGSPREMSEL
                     GETLYNKFNYSIIIPRLPGHGTNAEDFRLTDKKDWLRKVYDLILDNQNDYENIHLIGL
                     SMGALISLLASLHFKIKSMLLISPALYAKNKNIIFTHLLKYIRPVLSNSYEIDEEIED
                     PNLIELIKNYSCQEYSKQLSELHKLMFMARKNLKKITTKVKIIHSKKDELVPLKAAEA
                     IYEKISSHDKELTLFEDSPHVINYGPEKDKLFKEVSEFLNKF"
     gene            16360..17730
                     /locus_tag="VKN64_01500"
     CDS             16360..17730
                     /locus_tag="VKN64_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HMA58936.1"
                     /translation="MKKINITFNRAIIPVFSMIIFLILAVLLWDAPIHIPLIMELTFT
                     LFLALYWGFKWEELEEMMFSSFAKIGNVLIIIMLIGMIIGIWIESGTVPTMIYYGLRF
                     LRPQYFYIISFFFSAVVSISIGTAFGTVSTVGLALISIAQGMGISLPLAAGSIISGAY
                     VGDRMSPLSSIAIMTAHSADSDLHDMIKEMFKTFWAPFLLSAIIYFILGYRYNGFSYD
                     ISQINLLLDGLVNNYLISFMMLFPPLMIILLAVKRVPTILNLIINLILSSLIAFFVSG
                     MNIKEVIEALYSGASGQTEINLLNQLLTRGGLASMLDLVSLIILAVVLGGLLEKLGIL
                     NTILNKIIQAIDNRSQLISVTMASSFITAALSCNQLLGVFLPGKMLGSKYDEMEVPRS
                     ILGRSLGDSGLILSPLIPWNINALMMMGILGVHTLEYFKYTFFPVLVPITGLILANFI
                     NRGGKK"
     gene            17730..19154
                     /gene="pepV"
                     /locus_tag="VKN64_01505"
     CDS             17730..19154
                     /gene="pepV"
                     /locus_tag="VKN64_01505"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HMA58937.1"
                     /translation="MNNGLEKKVDSYQQDIVNSLQSIVKIKSVEAKAKEGMPFGEGVD
                     QSLRKFLEIAEELGLKTKYVDGYAGHVEIGEGEEMLGILCHLDVVPEGTNWTYPPYAA
                     EIHDDKMYGRGTIDDKGPAVASLYALKAVQESGIDLNKKVRLILGTDEESGWEGLDYY
                     FKKEPKPGMAFSPDAQYPVIHAEKGILIFDLNYELDIDVEEDYSKSTFKYEVLSLKGG
                     NAPNMVPDACEVVIKTDFEQAFEQKFKNFVDKTNYEFELEKINSNKYKVTSSGVSAHG
                     SMPAKGENAIARMMAFLGELDKVSIGEFVDFYNNKIGMDYYGERIGCKLTDEVSGKLI
                     FNVGQIKLEDNLVKTTVNIRYPVTKSKEEVLEGIKSSIDKYEAYLEMKNHQEPLYVPK
                     DDPLVKELMKVYKEVTGDEDSEPIAIGGGTYARAVPKAVAFGALFPGQEELAHQKDEF
                     IGLKDLYKNAKIIAYAIKALASKE"
     gene            19163..20086
                     /locus_tag="VKN64_01510"
     CDS             19163..20086
                     /locus_tag="VKN64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HMA58938.1"
                     /translation="MEVKLNNFPDIKVGNYTDQEAGTGCTVTLFDKPLAGSVSIMGGG
                     PGSRELALLDPTTRMDQINAVLLTGGSAFGLSAADGIMKYLEENNKGFDTGFAKVPIV
                     PGGVIFDLNYKHNGIRPGSNEGYYASTNADNKFEIGSKGVGTGATCGKLLGVDKASKS
                     GLGYSENNSDPIKVSAITVCNAVGNVVNPKTGEIVAGIKGESGYISYQDILESEIMDL
                     FGTNTTLGIVITNVKLTKAMLKKVAEMAQDGLARTVNPVHTMFDGDMIFAFSTNEVEA
                     DLNKTGILAAQSISDAILNGIAAVNNEKEGE"
     gene            20089..21375
                     /locus_tag="VKN64_01515"
     CDS             20089..21375
                     /locus_tag="VKN64_01515"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HMA58939.1"
                     /translation="MKHVNTDSLIEYLKNGPSPFHVVDKTADILENNGYQELKIQNKW
                     DIKENKKYYVIKNDSAIIAFNTGSKEKLKDGFKIVGAHTDSPSIKIKPQPEFKDEDYY
                     LKLNTEVYGGPILNTWFDRPLTLAGRVSVLEDGQLNNININFEDPLMVIPNLAIHLNR
                     EINKGQEIDKQKDLIPIMKTIKEDEEIEGYLIDMIARRLEVENDKIMDFDLYLRDTED
                     PIIMGSEQEFLNAGQIDDLGMAHAGLKALLNTENSGFKLLVLFDNEEIGSNTDVGADS
                     PLLSNLLERIVYALDGSREDYLQIIENTFMISADMAHAVHPNYADKHDPTNKPVINQG
                     PVLKISSNRRYTTDSYSGGYIKKLCAENNIPYQNFVNHSNERGGSTIGPLNLRHLGVK
                     SVDIGNPVLSMHSIRELYGLQDHESMIELLTVFYND"
     gene            21394..22590
                     /locus_tag="VKN64_01520"
     CDS             21394..22590
                     /locus_tag="VKN64_01520"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HMA58940.1"
                     /translation="MEYNFDKKIDRTGTDSIKWDMTDQYFDKKDCLPLWVADSDWETA
                     PEIKEAVRDRVEHGIFGYTYPSEKLTQTIIKWVKEHYNWQIKKEWIVYANGVVPSINI
                     SIRALTIPGDGVIIQTPVYFPFISTINNTGRQIVNNKLNNNDGYYTIDFEGLQENISP
                     DSEEGCKRAEMFLFCSPHNPVGRVWQTEELEKLNQIIIDNDLILVSDEIHADFVFADN
                     QHIPPASLSKEIENRSITLMAPSKTFNIAGLHTSFAVIPNKKIRKKFMSVKDHLVGAG
                     NIFGLTAMEAAYSKGEDWLEAQLDYLKGNIDYTANYLKENLPQIKFAKPEGTYLFWLD
                     FRNITNCSEDINEKLVKEFSVALEPGNWFGEGGAGFLRMNLATPRENIKRALEGIKKY
                     VDNYGK"
     gene            22604..23830
                     /locus_tag="VKN64_01525"
     CDS             22604..23830
                     /locus_tag="VKN64_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA58941.1"
                     /translation="MNDVIVIGGGPAGMMAAGTAGSRDKKVQLFEKNEKLGKKLYITG
                     KGRCNFTNKADVNTLIENVVNNSSFLYSAFYSMDSERLIKFFEDLGLKTKVERGNRVF
                     PRSDKASDVTKTLKKYLKENNVEVKYKKVQEILTSDNQITGVQTTDGKVYNCESVVLA
                     TGGYTYRSTGSTGEGYEMAKKLGHTVNELYPSLVPLELHENYETKKLEGLSLKHVEIK
                     IRKPDNQIIYQDFGDMVFTEQGVSGPIILSASSNLKKVKDKNYILSIDLKPALDYETL
                     NRRLQRDFKKYANKDFKNAFDDLLPSKLIPIFLKRTGIDLRKKVNQITAEEREKIISL
                     LKEFNYTIKDYSSFNEGIVTAGGVEVNEINPSTMESKIVKGLYLAGEMIDVDAYTGGF
                     NLQIAFSTGYLAGLNV"
     gene            complement(23913..24293)
                     /locus_tag="VKN64_01530"
     CDS             complement(23913..24293)
                     /locus_tag="VKN64_01530"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HMA58942.1"
                     /translation="MSVIEKAKELGQELVESEEYKELKRKEKALYDDEDATSLLEEYE
                     NINKQLQMARANGQEVNDQQQKKMQSLQMKMEQNPSIKSYIESQKKFNEVMNSVNKII
                     NGYITEQEQEQEAAQDQGSSNIIT"
     gene            complement(24392..25897)
                     /locus_tag="VKN64_01535"
     CDS             complement(24392..25897)
                     /locus_tag="VKN64_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMA58943.1"
                     /translation="MIKKLFNINSILGRLMVSYLIVIFISLAVIGIMFGYLVQNYYNG
                     LKEWEATNNSRRIATLVSENIGEGNILLTDDFSIGSTRSKINTIARSTNMDIGLIDKT
                     GEMVLNVPSLEANLSLEQEELNQVLSGNTFTKKIMGPDYRHLLMAVPLFQTDEEDVKI
                     AGNQPGAEKNNIIGAVLIQTPLGNIGATINNIMKLILYSFLVALAAAVFLSISFSRRI
                     TRPIDRIKEAALKSTEGNVEKVDLPENSTEEINHLANTYNYAAKQINRTLDKQRALEK
                     MQKQFVADVSHEFRAPLTSIKGFLEILKEQDLSKEERAEYLDIMFKDTQHLEKLLNDL
                     IELSKLDTAKESLDKKQTSPKILVNGALKSLESIIEEKNIEIVKNIEEDLPEINIDKN
                     KIHQVLINLIENAVNYSDPNSKITITVEEADQSNYQVEFSVIDNGVGIAVEELENIWE
                     RFYKIDTARTRDEEKGSGLGLAIVKDIIEKHDGEIEVESELDQGSKFTFKL"
     gene            complement(25890..26615)
                     /locus_tag="VKN64_01540"
     CDS             complement(25890..26615)
                     /locus_tag="VKN64_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA58944.1"
                     /translation="MDLYNKKILVIEDDEHIFAVIKAMFKQYDNNSLILKKNGKEGLK
                     EALQNEYDLLLLDIMLPKKDGWEICKELKEKKNDLPIIMLTAKSEEADKVLGLEIGAD
                     DYVTKPFSPRELTARIKAVLRRYEKTESKKTDTLYFDSLNMEIDTDSYTVTIDEENVS
                     FTPKEFKLLSFLAQNKEQVFTREQLLENIWGFENLTETRTIDEHIKRIRKKLNNTGIE
                     FQPIKTVWGVGYKFSIRKENNND"
     gene            26793..27035
                     /locus_tag="VKN64_01545"
     CDS             26793..27035
                     /locus_tag="VKN64_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HMA58945.1"
                     /translation="MIFGKNYNDELLQKEAKNAIEEQPTLRGIPNINVTSDNGIVSIF
                     GSVRTEELKKDIKKIIENKYDEKNIDYKEIQNDLTI"
     gene            complement(27077..>28223)
                     /locus_tag="VKN64_01550"
     CDS             complement(27077..>28223)
                     /locus_tag="VKN64_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA58946.1"
                     /translation="KNNDLKEPANRFAVVIPAHNEEKVIDKLIENLRNLNYPKDLYDI
                     YVVADNCEDDTAQKAKEQGVNVLSRFNQEKKGKGYALRYAFRSLGFLNGKTDYDAAVV
                     FDADNLVQENFLKAMNTRLLQGEKIIQAYIDSKNPADNWVTATFSMMFWINDRYNLLS
                     RFNVGLSSVLMGTGMCISQESLSKIGWDTVTLTEDLEYSIQALLKDTKTTFARETKIF
                     DEKPVSFYASCRQRLRWGRGQLSVLFKYIPKMLMQGIKELNIVKIDSAMRLIQQPFLM
                     TYFVVTILRLTFPGTFNSPLFNLFIQNIKTLGFILPIMPFIIPSSVYLLDNLSFDSFK
                     YVIFFPIFMYSWVLILYYALFTLNNKSWLPTEHFRSVSHEELQKNMK"
BASE COUNT         9652 a         4410 c         5697 g         8464 t
ORIGIN      
        1 agctgaaaaa aggttagaag aatatggtga aaataagctt gtccgcttaa agaaaacacc
       61 tgaaatcata aaattcctat tacagtttag agatgtacta atgatcatcc tcttagtggc
      121 agctggtatg tcctttatta tccaaaatta ccgggacggt ttggttatgt tagttatagc
      181 cttaataaat gctgtcatag gatatttcca ggagcacaag gcggaagcca taatggaatc
      241 attagagcag cttatccaat ccccctcgaa ggtaatacgg gagggacaaa ctaaagaaat
      301 tagtcaaaat aagttagttg taggagatat agttgttctg gaagaagggg ataaagtacc
      361 ggctgatttg agaattatcg aatcatttaa cttaaaaaca aatgatgttt cacttacagg
      421 tgaatcacta ccccagggaa agaatagtaa taaaatggag gaagaattat ccttagctga
      481 tagagagaac atggcctatc tgggtacaaa tgttgcttcc ggtagtgcca aaggtgttgt
      541 ggtagcaacc ggtatggata ccgagatggg taaaattgcc aacatgaccc aggaagaaga
      601 aaaatctaaa tctcccttac agatggaact gcagtctgtg gctaatagaa tagccgtttt
      661 tgcagtaata atcggtaccc tgctttttgg agcaagtatt taccagggtt taggttttga
      721 atttgcaatc atctatggat tggggattgc cgtagcggtc gttccccagg ccttaccgat
      781 gcagattacc gtggccctag cccagggtgt cgatagattg gctaaaaaga acgcggtagt
      841 taaaaaactt tcctcggttg aaacattagg ttctaccaat gtgatttcaa ccgataaaac
      901 gggtacctta acaaaaaatg aaatgacagt gcaaaaggta tattacgatg gtcaagaata
      961 tgatgttacg ggaatcggat atgaaccgcg tggaaagatc ttagaccagg atggtaatga
     1021 agtaagtcag gagatcattg accatttaga aatcatgttc gatgccgcga ctatggcatc
     1081 caatgcagag atccatccac cagatgatga acattcaagc tggtatccga tcggagaccc
     1141 gaccgaagca gctttaatta ccttatcgac caaagtcggg accagggcag aaaatgaaga
     1201 tgaagaaaac ccggaattac atgaatttag ctttgattca gaacggaaga gaatgagctc
     1261 ggtccgtcag tttgaagatg gagaatacct ggccatgaaa ggtgctttag atagtgtcct
     1321 ctcagtcagt aaatatatct ataaagatgg taaaaaggtt ccgattacag aggaagataa
     1381 agaaaaatat agaaagttaa atcaaaagta ttccgaaaat gcgatgcgtg ttttagcttt
     1441 tgccttccgc aaactgggac cagagcataa agactatgtt ttagaggaag ttgaaaaaga
     1501 tgtaattttc cttggcattg tggccatgat tgatccacct aaagaagggg taagagaatc
     1561 aatagaacag gcccatgcag ctaagattaa cacctatatt atgaccggag accattcaac
     1621 tacagcaaaa gccattggta aagaaataaa tctggtcaaa gatgaagaaa atattgaagt
     1681 agtaaccgga aaagaatttg ataatttatc agatgaagat ctaaagacaa tgatggatga
     1741 aaatgaaagc cttatctttt ccagggtttc gccggaacac aagttaagaa tcgttaagaa
     1801 ccttaaagat cagcaaaaga tagttgctgt taccggtgat ggtgtaaatg atgcacccgc
     1861 cttaaagagt gctcatattg gagtagcaat gggtggaatg gggactgatg tctccaaaca
     1921 ggcggctgaa ttgattctat tagacgatag ttttcccacc ttggtgtatg cgattaaaga
     1981 aggcagaaac atctataata acttaaagaa aactgttatc gcctccttaa ccagtaatgg
     2041 cggagagcta gcagttgtac tattaggatt actgggtgtc tcattattta atttaccggt
     2101 acccattttg gcgattcaaa ttctagctat tgacctttta gctgagattt taccgttgac
     2161 cgcctttact tttgatccac cttctaaaga tataatgaag tcaccaccaa gagataagga
     2221 tgagcatatt gtaaataaga ggtcattttc tgaaattgcc ttcttcggtc ttttgatggg
     2281 tttcttagct ttcttaaact actacctatt tagcagtaga atgggtatca tggatatgac
     2341 agaagcacat cccctatatg ccagggcaac tacgatcacc tatttgacaa ttgcttttac
     2401 acagctaaca aacatcatgt cgagaaggta tgaaaaggtt actttcttta ataagaacat
     2461 tttatccaat aaaaagatgg tttattcaat gatcctgtca atcttgatga tagtaacagc
     2521 aatctataca ccctttatca atcaatatct aggttttgca ggtataacat tcgtagattg
     2581 gctttatgta ttaggttccg ctggtatttt cttaggagca catgaactat tgaaagtata
     2641 taaaagaagt agtgagaact attaaaaagg aggctaaaaa taatgaatgt aaagaaaaaa
     2701 ggagaagttt acaaatgtgg agtctgtggt aatgtggtag aagttaaaga agttggtgga
     2761 ggcgaattag tctgctgtgg tcaacctatg aacttacaga agtaattagg agaacgcccc
     2821 tgtttagcag gggccatatt ctctcacaac tggggtgatt acatggaaaa atttaaagtg
     2881 aatcagactc aggttgtttt aaaacaggga gatattacag aagaagcttg tgatggaatt
     2941 gtcaatgctg ccaactcctc actgatggga ggcggtggtg tagatggagc aattcataga
     3001 gccggcggac cagaaatcct agaagaatgt aaagaaatta gggaaagaca gggcaaatta
     3061 cccaccggta aagctgtcgt tactacagct ggtaaaataa aggcagaagg tgttattcac
     3121 accgtgggac caatttggaa tggtggagat tctaaggaag aagaattatt agcaaacgcc
     3181 tatagaaata gcctaattgt ggcagaagaa aactcatatc aaacggtgtg ttttccttcc
     3241 ataagtaccg gtgcctatgg ttttccgttg gccagggcag ctgaaattgc cttaaataca
     3301 attaaggact atatccaaaa caatgaccat ttcaagcaag tccgcatgat attgtttagt
     3361 gaagaagatt ataatattta tttaaaaata gccaaagata tttttaaata aattagacta
     3421 taaatgtgaa taaagaagga gtgttgacaa tgagtgtaaa acaagatacc ttagaaatga
     3481 acaattgggc cgtagtcggt gcaactgata aggtagaaaa atacggctat aagattgtaa
     3541 aaactctgga tgaacatggg tataaagtat ttccgatcaa cccatcttta gatgaaattg
     3601 atggtattaa agtatatgat aaattagctg acattgaaga gccagttgat gtagttgata
     3661 tggtagttaa cccacagata ggtaaacacg tgatgaagga aattaaaaaa ctgggtataa
     3721 aaaatgtctg gttacaacca ggtactcgca gtgatgaaat tagagaattt gcagctgaaa
     3781 atgatattaa tgtagtcgaa agctgtattt atgcaacttt aaaaagtaga taattaataa
     3841 aaaagaaaat aggagggaac ttaccttgtc taagtatatt cttgcaattg atcagggcac
     3901 aacttctacc agggccctat tatatgataa aaatggtaat atagtagaaa aggctcaaaa
     3961 agaatttact cagtattatc ccaaaccagg ctgggttgaa catgacccgg aagaaatttg
     4021 gaattcaact atcggtgtta tcaataatgt aattaaaaaa agtgagatca agccatatga
     4081 tatagattca atcgggatta caaatcaaag agaaaccacc gttgtttggg ataaaaatac
     4141 aggagaacct ctttataatg cgatagtctg gcagtgtaga aggacgtcag acatctgtaa
     4201 aaatttaaaa gaagaaggtt atgaagaact ttttagagat aaaaccggcc ttctcttaga
     4261 tccttacttt tccggtacaa aaatcaagtg gatacttgat aatgtagaag gcgctagaaa
     4321 gaaagcagag gagaataaga tattatttgg tacaatcgac acttatttaa tctggaaatt
     4381 aacggatggt aaagttcatg taactgatta taccaatgct tccagaacct tattatataa
     4441 tattcacgat cttaaatggg atcaggaact attagacatt ttggatgttc cctcctatat
     4501 tttaccagat gtcaaatcat cgagtgaaat atatggttac acagataaaa acgtattttt
     4561 tggacagcat attccgatcg ccggtattgc aggggatcaa caggcagcca cctttgctca
     4621 gggctgctat gaagaaggaa tgacaaaaat aacatttgga acaggtgctt ttcttttaat
     4681 gaacactggt aatgaacctg tctattctga aaatggttta ttaacaacta tcgcctgggg
     4741 gtgtgacggg gaaataactt atgctctaga aggtagtata tttaatgctg gttctgccat
     4801 ccagtggtta agagatgaat tatttctctt aaatgaagca cctgattccg aatattttgc
     4861 cggtaaggta gaaagttctg atggtgtata tttcgtaccg gcctttaccg gtttaggcgc
     4921 accttattgg aacccggaag tgcggggaac tattgttggt ttaaccagag gcagcagtaa
     4981 gaaccatatt attcgtgcta ccctggaagc tttagtctat caaagcaagg acttaattca
     5041 ggctatgctg gaagattccg gtttgaagtt aaaagaagta aaggttgatg ggggtgcctc
     5101 ggccaacaat ttattgatgc agtttttagc tgatatgact aatacaaaag ttgagagacc
     5161 tgttaataca gaaactacag ttactggggc agcttattta gccggcttag caactaattt
     5221 ctggaacgat cttgatgacc ttatgaaaaa aaggaaagta gaaaaggtat ttactcccaa
     5281 aatggaagaa accgaaaggt ccgaacgtta tgagggttgg ttaaaagcta tcgatactgc
     5341 cctctactgg tcagaagaat aattctggat taaaagaagg aaatatttat tcagtataga
     5401 atataatatt tagaaaattg tagtaaataa aataaaattt taaaaagtaa tctatttatt
     5461 taaagaaaat ttagtgtgta gaaagttaaa aaattagata tttggggtta gagaaaagag
     5521 aacctattta atgtatttcc ataggatatc tatgggtaga tttaacatta aatgggtttt
     5581 tttatttttg aataaagggg tagagtagtt tatgttaaaa aatctatttt caaaccatgc
     5641 acaggaatta caaaatgata ttaataagta tctagaagta gtgactaact ctaatatggt
     5701 ctttaaacga agttgtaaaa attatttaaa ggacaatatc gaatcatttg aagactcttt
     5761 ggatgaaata aataagcttg aaaattcagc agatgatttt caaaaagata taaagtataa
     5821 attatataaa tacatgttga ttccggaagc aagaggggat gtattagcct tattagaaaa
     5881 catcgacaat attgttgacc atattaaaaa acttttagta cagttttcaa ttgaaaggcc
     5941 cactatacct gatagtttaa acgaagatgt ttctgaattg gtcgatatgg taagtgattc
     6001 tgtggatgta ttgataaaat gcataagagc atattttaaa aatatttcaa tggtcaatga
     6061 atatgccaat aaagtacatt tctatgagca tgaagctgac aagatagaag agagcgtaaa
     6121 aagaaaagtt tttgaggata gtaatttaga gtacttcagc caaaaaacac atttgcgata
     6181 ttttgtagaa cagatcactg agatttccga tcgggccgag acaataagtg aaagggcttc
     6241 ggttgcggcg attaaaagga gaatctaatt aatgtgagtt gatattatat taaaaaacat
     6301 ttttttcaat ttatttttta gatttaaaaa gttaaataat attattaata aaaagtaagg
     6361 ggactatatt atatggataa gattaaggac acaaagttat ttatatttga tatggatggt
     6421 accatatacc taggagataa tctaatagag ggcgtgcttg actttatagc taaattaaaa
     6481 aaagagaaca aacaatatat ctttttaaca aataattcat ctaaaaattc gaaagattat
     6541 aaagagaaat taaataaact gggtattcaa gctaccacag acgatattgt aaacgcggga
     6601 gaggtaacag ccgattacct taaaggcata aaagccaatg gggaagacaa tgtatatgta
     6661 gtgggtactc cttctttgga aaaagttttt gaggattacg gcttcaattt gataaaagat
     6721 agagataaaa atgtggactt tttggtaata ggctttgata caaccttaac ctacaaaaag
     6781 ttatgggatg ctcattatct tattaaccag ggtgttaaat attatgccac taatccggat
     6841 aaagtctgtc ctttagaaga cggtaggtct atgccggact gtggttctat aattaatctc
     6901 ttaaagactt cgaccggtca agaaccggta gtggtcggaa agccgtctaa attgatggta
     6961 aattatattt ccaacaaata tagtatagat aaagataaaa taagtatggt gggggaccgc
     7021 ctctatacag acatcaagat ggccaaccag agcggtataa acagtatatt agttttatcc
     7081 ggtgaaacaa aacaggaaga tctcaaatgt gctcagatac aacctgattt tgtcctggac
     7141 tctgtcgttg aaattggaaa tatgttctag ggagggatgt agattggaaa caaagattat
     7201 tgcccatagg ggctcttcga ataaagcacc cgaaaatact gaggtatcat tccgccaggc
     7261 ggtcaatgat aaagcggatg gggtagaatt tgatgtacat ctaaccgctg ataaagaagt
     7321 agtagttatc cacgatgaaa aaatagatag gacttccaat caaagtggtt atgttaaaga
     7381 ttatacttta aaagaactaa aaagatttga ttttggtagt tacttcgcaa aaagatttaa
     7441 agaccaatca atacttagcc tggaagaaac actagatata gttcaaaata tggagctaat
     7501 taatatagaa atcaaaaaag gctatggtat aaaccagggt attgagaaaa aaatagttga
     7561 gataataaaa ggaaaaggtt tattagataa agttttaatc tcctccttta accatgactc
     7621 tttaaaaatc attaaagata tagacagcaa gataaaaacg gccgccttac tatttgcacg
     7681 attacataaa ccctggcagt atgccaagaa tttgggctgt gactacttgc acctatatta
     7741 taaattactg gataaagaaa ttatagatag ttgtcataag aaaaatgtga aggtcaatac
     7801 ctttaccgtt aatgatatgg ctgatatgca aaagctaatt tatattaatg tggatggaat
     7861 tatcacagat tatccgatgg aagcgttaaa attattagaa gaagtaaact cttagatggg
     7921 agatggtact atgcaaataa ttgtaattgg agcaggcgta atcggctctg ctattgccag
     7981 agaattagca agatatgatg ttgaaatact attaattgaa aaagaaccag atgttgccga
     8041 tggaaccagt aaagcaaatt ctggtattat tcacgccgga tataatgcag actttgatac
     8101 cttaaaagga gtaatgaatg ttaagtccaa cccttctttc gataaattat gtgaagacct
     8161 taaggttcct tttgatagaa acgggtcttt agtggttggt tttgatgacg atgatttaaa
     8221 gaaattaaaa gagaaaaagg ccaacggaat aaaaaatggg attgaaggct taaaaattat
     8281 cgaacgggaa gaaatccttg aaaaagaacc gaacataaat aaagaggcta aatatgccct
     8341 atatgccccg accgccggca taatctcacc ttatgaacta acgattggtt atgccgataa
     8401 tgctgtccga aatggggctg aagtattatt agatacacaa gttacagact taattattga
     8461 agatgatgaa gttaagggtg tacttaccaa caggggtgaa tttagggccg atctagtgat
     8521 caatgcggcc ggactatact cggataggat agctaaaatg gccggtgatc agtttgagat
     8581 acaccccaga aaaggagaat accacctttt tgataaggta cacggagata tagtaaacca
     8641 taccctattt ccaatgccga cagaaacctc gaaaggtatc ttagttttac ccactgtcca
     8701 tggtaacttg ttaattggac caaatgccaa tgtgatagat gataaagatg atttttcaac
     8761 tacttccgat ggtttagaag aggtctttaa gggggctaaa aagttggttc cttccttacc
     8821 agaaagtggg gtaataacat ctttttccgg tttgagagcg gctttaccga gtgaagactt
     8881 tcatatcggt ttttccgaaa acaaaaaagc tttaattaac ttaattggaa ttcaatcacc
     8941 gggcttaagt tcagccccgg cgatcgctga taaagtagtt gagatggccg aacagtttag
     9001 taaaaataat aatattgaat ttaataaaaa ggacgatttt aaagaagcca atccacgtta
     9061 ccctcattat catcattatg aagaaaacga caaggtagaa gagtggcagg agatcgttaa
     9121 acaagatgaa aaatatggag agattatctg ccgctgtgaa catgtaagcc ggggagagat
     9181 tattgatgct ataaatagac cggttccggc tagaacagta gatgcaataa aaagaaggac
     9241 cagagccagt tcgggccgct gtcaggctgg cttttgcgga ccacggattc tagatatttt
     9301 atcaaatcat ttaaagatag atcccctgga agtaaccaaa aaaggtaaag gttctaaaat
     9361 actaaaggca aaatctaaag aaataatact aaaaaactta gaagaagata gtaaagtagg
     9421 tgatcagtaa atgcaagaat ataatatggt ggttattggg gcaggtccgg ctggaatggc
     9481 tgctgccctg gaagcagaaa aaaacggtct aacagatata gtaattttag aacgtgatga
     9541 atatataggt ggtatcttac cccagtgtat tcacaatggt tttggcttag aatactttgg
     9601 ggaagagtta accggtcctg aatatgccta tgaatttata gatgaacttg cagattctaa
     9661 tatagaagta ttaactgaaa ctatggtttt agatgtggat gaaaacaaag aggtaactgc
     9721 cttaaataag gaaaaaggtg tattaaaact aaaaggcaag accattgtct tagcgatggg
     9781 ctgtcgggag agaaccaggg aaaatatccg gatacctggt gatagaccgt ccggcatact
     9841 ttcggccggt actgcccagc gatatacaaa tatcgaaggc tatatgccgg gtgaaaaggt
     9901 tgtaatcctg ggatcaggtg acattggact tatcatggcc agaaggatga gattagaagg
     9961 cgccgatgta aaggctgttt tagagatcat gcctttttcc tccggactgg ttagaaataa
    10021 ggtgcagtgt ttagatgatt ttgatattcc tttaaaatta cagcatacaa taaccagaat
    10081 tgaaggacag gaaagattag aacgggtcag agttgcccag gttgatgaag actttaatcc
    10141 gattgaaggg acccaggaat ggatagaatg tgatacttta ctattatcgg ttggtttaat
    10201 cccagaaaat gaattatcaa aggcaatggg actggacctc caccccgtaa caggcggtac
    10261 ctttgttaat gagaataggg aaacaagtat cgaaggagta tatgcggccg gtaatgtctt
    10321 gcatgtgcat gatctcgtgg actgggtttc agaagaagct gaaattgccg gtaaggctgc
    10381 cgctgattat atcaataaca acctagaaaa aagagcccaa aaaatagaga taaccccggc
    10441 tgaaaatgtg acctatacag ttccccagac catagaatat acttttggag ataaaaaatt
    10501 tattaaagta tttatgaggg ttactgagcc gatggaagat gtagaagtga aattttcagc
    10561 taataaagaa gagataatca gttatcctaa aaagatcgtt atgccgggtg agatggtaac
    10621 tgccaatcta ccagctgccc ttgtaacaga tgagatggaa gagatcaaag tctcaattgt
    10681 taaaaaggcg ggtgaaaata atggctaaaa atgatataat atgcacaggt tgccccaaag
    10741 gctgtcatgt tacagttgaa gtagaagatg atgagatagt aaatgtagaa ggttatgaat
    10801 gcccgaccgg gcgtgaatat gccattaatg agtataaaaa tccgaccagg attcttccct
    10861 ctacaattag agtaaagaaa ggagaattcc ccctggtccc ggttaagacc gcccaaccca
    10921 tacctaaatc tcttttaatt aaagcaatgg atgtaatagc aaaaacagag gttaaggccc
    10981 ccattaaaat aggagatgtc ttaatagaaa atatactgga tacgggagta gatatagtgg
    11041 caacccgcaa tatagataaa gaagtggagg tttaagcatt gaaagaaagt ctcgaagaat
    11101 atttcgcaga ctttaaagta atagccggga taagtgatga ctctcgctta gaaaaggctt
    11161 tatttagtaa agtattggga gtatttttac tcaacgcaaa attaaataag ttaaatgaat
    11221 atgcagaaaa tgtaaaagaa cataataaga agttgttcct acatctggat cttatgaaag
    11281 ggatgaactc ggataaagaa gctgttcatt acctggcatc taatgacctc tgtgatggta
    11341 taataactac ccacaaaaat ttgattaagt atgccaaaaa ggaaggtcta tatacagttc
    11401 agaggatttt tgtactggat tcaggtacta ttaagaacgg aataaaaagt ttaaaaacag
    11461 ttagacccga tgccgtagag atcttaccgg gtctcatggc ccctcatttt attaacgaat
    11521 tatcaatgga acttgatatt ccatttattg caggcggttt aataaggaca agagaagaag
    11581 taaataaatt attaaaagac ggtgtatttg ccgtttcaac aagtgaagat gaactttggt
    11641 attaaaatat tgttaaaaca gggataagtg atatttgctt atcccatatt tttttggact
    11701 tattaaaagg gatttgtcta aaattcaaga atatatataa ggaattgtat aaaaatattt
    11761 aatttttaac aggagggtta ttaaaaaata atggattatt ttaaaaagag caagataagt
    11821 aatattttac agggtaagat tgattcatac gaagatgtaa ttgtactagg ttgggtaaag
    11881 acaaaaagag tttctaaaaa tattgcattt ttagaagtta atgatggttc aacagtgaaa
    11941 aacttacaga ttgtagtttt agaaccatct aactttgaat tagaagatat aaatagaggt
    12001 tctagtgtcc gaattaaagg ctatctggaa gatgtgccaa accgtgaaca gagtattgag
    12061 atgaaagcgg aagagataga tatcatcggt actgcacctg aaaattatgt gcttcagaaa
    12121 aaggaacata cctttgaatt tttaagagaa atagctcacc taagaccaag gacaaagacc
    12181 tttagtaccg ttaataggtt tagaaataag atggcctatg gtattcataa atactatcag
    12241 gaaagaggtt tccaatatat tcatgccccc atcattacag ctagtgatgc agaaggggcg
    12301 ggtgaaatgt ttaaagtaac caccttagat ttagataata taacgaaaga tgataaaaat
    12361 aaagttaatt tcacggaaga cttttttgga gaacaaaccg gcctaacagt aagtggccag
    12421 ctggaagcag aggttatggc aactgcctta ggtgatgtat atacatttgg tcctaccttt
    12481 agggctgaaa actccaatac ttcccgtcac gcatccgaat tttggatgat ggagccagaa
    12541 atggcctttt gtaacttaga agaaatgatg gatattatag aagactttat aaagaatctc
    12601 ttcgaatatg ccctcaatga agcggccgaa gagatggaat ttttcgctga aaatattgaa
    12661 gaaggtagaa aagagactct aaaaaatatt ataaattcta cctttgccag aatcacctat
    12721 acagaagcaa tcgatatctt aaaagaagcg gaccaggatt tcgagtttcc tgttgaatgg
    12781 ggtatggact tacagaccga gcatgaaaga tacctaacag aacaacacta tcaaaaaccg
    12841 gtcttagtaa cggactatcc caaagagatt aaggcatttt acatgcgcct taatgatgac
    12901 caaaaaactg tggctgcagt tgattgtctg gtaccagaag tcggtgaaat tatcggcggc
    12961 agccagaggg aagaaagata tgatgtgctt aaagaaagaa tggaagaaga gaatatgaat
    13021 attgataaat atgattggtt cttagaactt agaaaattcg gaacagtgcc tcattctggt
    13081 tttggtctcg gttttgaaag gatcttgatg tatatttccg gtattaaaaa tattagagac
    13141 gtcattccat tcccaaggac tcccggtaat gctaagttct aaatacaggt ttttaatttc
    13201 attagtaata atattacttt taactacacc aattttatca gctcaaagta atcttacttt
    13261 ggactatgat gttgtagtgt atagtggaca gccgggcggg gtaacggcgg ccgttagtgc
    13321 cgcccgcagc ggtctgtcca ccctcttgat cttaaaaaga gataatccag gtggtttaat
    13381 gacttatggt ggtttaaact acctggattt aaattttgat aaggctggta aaattttgaa
    13441 taaaggacta tttgctgaat ggtatgagag aatcggtaaa aaccaaagtt ttacttatga
    13501 taaagccacc caggtattta atgaaatgtt aaatgaggcc ggggtagtgg tgattaagaa
    13561 ttctaattta gaaagagttt gtcttaaaga taataatatc gagaagatgt tgatcaaaga
    13621 tgagaaagaa aactactttc aaattactgc tgattacttt atagatgctc agcaggatgc
    13681 tcagttagct tttaaggcgg gcgccaattt atttaatttc ggttctgata ttaatttaaa
    13741 tgaccgcttt atggccatga ccttagtatt aaatatagac gatattaaca ttaacaatct
    13801 aaaaaaggac attgttagta atagatatgg gccttcctat ctaaaaaatg ggcatgccta
    13861 tggttttaaa gaactcggta agatatatga accacataat gaaaacctta agttaagggg
    13921 attaaatata atatttaatg aaaatcataa tggttaccac cagggttcta taaatgccct
    13981 tttaattttt aaccataatc ctcttgacca aaaagaaagg gaagccgcct atcaattagc
    14041 agctgaagaa gcaaaatata tcctggaatt tttaaaggaa aagttaaacg gatttaaaaa
    14101 agcgaagtta aatgagccgg ctgaggaatt atatataaga gaatcacgtc atttagtggc
    14161 tgagaatatt ctgcaggtag aggatttatt agaaaataaa atatctgata atactataac
    14221 cctggcttct taccctctgg actatcaggc ttcagagatg gattatcagg gctttgtcct
    14281 ctttaaccca gttgtgtatg gtataccctt tgattcctta ttacccgaca atatcaggaa
    14341 cttaatggta gtatcccgca gtactggtct gagctcacgg gcagcatcca gcgctagagt
    14401 cataccggct aggatgaaca gtggagcagc ggcaggcaag gcagccgctt tggccgatga
    14461 aaacgagatt agtttaaaag aattaaataa aaataagaaa ttaatagctg aaattcaaca
    14521 gtatatggag attaatatta gtgagtataa agatataaaa cctttattta aaaaggataa
    14581 cacctatccc tatatcaaag aattatttaa tctcggctta ataattgggg gctatcaaaa
    14641 cgaatttggc tttgattcta ccatgtcaga atatgaattt ggtcagcttt taatcaaggg
    14701 catgattaga aggaattctc ctatcttcta tgagtgggta ccggggagtc tggaaacact
    14761 tacaacttta aaaaagctgg ataaactaaa tgcctataaa ctaatcttag cagccagcag
    14821 tcatcaagtt ctagagatag cagaagaaaa atatttagat tatttaagag aagacaactt
    14881 gatcccgacc gattttgaag agagtgttaa aagcaatcgg gtcttaaata gaaaagaagc
    14941 ctattaccta attggatatt ttttaaaaca gagtccttta cccaataagt taaaaatatt
    15001 gaggggaaca aaaaaatgag caaggaagta aagtacctaa aatccccgct cggcttattg
    15061 aaaataacgg taaaacatga taaattgata tcaattaaat acgtaaaaga aaaaggaaag
    15121 caattggtca aagaaataaa ttctgaagta atgaaaaaga cattaattca gttagaagaa
    15181 tattttaatc acaagagaaa taactttgag cttcccctat caattgaggg tacagatttt
    15241 caaaaagatg tttacaaagc cctcttaaaa gttaattatg gaggagtgat ttcttataaa
    15301 aaattggccg tcaactccgg ccatcccaag gcctatcggg cagttggaac cgctttaaag
    15361 aaaaataaga tccccattgt aataccctgt catcgggtaa taaaatcgga tcacagtctg
    15421 ggtaattaca ctggtggtaa atggagaaaa gaagagctgc tggagttaga agaatcagaa
    15481 tttatttaaa aattcactga cttccttaaa cagtttatct ttttctggac cataattgat
    15541 tacatgcgga gaatcttcaa acagagtaag ttctttatcg tgagaactga ttttttcata
    15601 aattgcttct gctgctttaa gaggtactaa ctcatctttt ttggaatgaa ttatttttac
    15661 ttttgtagta atttttttaa ggttttttct tgccataaac atcaacttgt gcagttctga
    15721 taactgtttt gaatattctt gacaactgta atttttaatt aattcaatta aattaggatc
    15781 ttctatttct tcatcaattt cataactatt tgaaagtacc ggtctgatat attttaatag
    15841 atgtgtaaaa ataatgtttt tgtttttggc gtaaagggcg ggcgaaatta ataacatgct
    15901 tttaatttta aagtgaagag atgctaataa agagattaag gctcccatgg aaagacctat
    15961 cagatgtata ttttcataat cgttttggtt atctaaaatt aagtcatata cttttctaag
    16021 ccagtctttt ttgtcagtta aacgaaagtc ttcagcattt gttccatgcc cgggtaaacg
    16081 gggtattatt atagaatagt tgaatttatt atataaagtt tcacccagtt cagacatttc
    16141 cctcggtgaa ccggtataac cgtgaattaa gataaccaga tctttttttg aaccttttaa
    16201 tcttacttct tcagccaggg gattaacttc taaatttata tcaggtagtt tatacattat
    16261 gagacctcct aaaaacaaat tctaaatatt cttttttaat agattcttga atatgaaaaa
    16321 taatccttta taattttaaa caaaattcgg tgattaaaaa tgaaaaaaat taatataact
    16381 tttaacagag caataatacc agttttttct atgataatat ttttgatatt agcggtatta
    16441 ctctgggatg cacctatcca tatcccctta atcatggagt tgaccttcac cctcttttta
    16501 gccctttact ggggttttaa gtgggaagag ttagaagaga tgatgttttc cagttttgct
    16561 aaaataggta atgtcttaat tattataatg ttaatcggga tgattatcgg gatctggatt
    16621 gaaagtggaa ccgtccccac catgatctac tatggtttga gatttttaag accacagtat
    16681 ttttatatca taagcttttt cttttcagct gtagtatcga tctcaatcgg gaccgccttc
    16741 ggtactgtaa gtaccgttgg gcttgccctg atcagtattg cccaggggat ggggatttcc
    16801 ttaccattag cggccggttc aataatttca ggtgcctatg tgggggatcg aatgtcaccc
    16861 ctttcatcga ttgcaatcat gaccgctcac agtgctgata gtgacctaca cgatatgata
    16921 aaagagatgt ttaaaacttt ttgggcgccc tttttattat ctgctattat atatttcata
    16981 ttaggttatc ggtataacgg tttttcttat gatatttcac agatcaactt acttttagat
    17041 ggattggtaa ataattatct gatttctttt atgatgcttt ttccgccgct tatgatcatt
    17101 ctgcttgcgg ttaaaagggt accgacgatc ttaaatttaa tcataaattt aatcttaagt
    17161 tcgctgatag ctttttttgt aagtggaatg aatatcaaag aagttatcga agccctctat
    17221 tccggggcga gcggccaaac cgagattaat ttattgaatc agttgttaac caggggggga
    17281 ctggctagta tgctggactt ggtttccctg ataatcctgg cggttgtttt aggcgggctc
    17341 ttagaaaaac tgggaatttt aaacactatt ttaaataaaa ttattcaggc tattgataat
    17401 agaagccagt taatttctgt gacgatggcc tcatctttta tcacagctgc cttaagctgt
    17461 aaccagttat taggggtatt tttacccggt aagatgctgg gcagtaagta tgatgagatg
    17521 gaagtaccgc gcagtatttt gggtcggtcg ctaggagatt ccggccttat tttatcacct
    17581 ttaattccct ggaatataaa tgccctgatg atgatgggta ttttaggggt tcataccctt
    17641 gaatatttta aatacacatt ttttcctgtc ctggttccca taacaggttt aatcttagcg
    17701 aactttatta acagaggagg caaaaagtaa tgaataatgg tttggaaaaa aaggtagaca
    17761 gttatcaaca agatatagtt aactctctcc agtcaatagt taaaattaaa agcgtagaag
    17821 cgaaagcgaa agaaggaatg ccttttggag aaggggtaga tcagagtctc agaaagtttt
    17881 tagaaatcgc agaagaatta ggacttaaaa caaagtatgt agatggttat gccggtcatg
    17941 tcgaaattgg tgagggtgaa gagatgttag gaattctctg tcaccttgat gtagtaccgg
    18001 aaggtacaaa ctggacttac cctccctatg cagccgaaat tcatgatgac aagatgtacg
    18061 gccggggtac aattgatgat aaaggtccag ctgttgcttc tctttatgcc cttaaagcag
    18121 tgcaggaaag tggtatagat ctcaataaaa aggtccgctt aattttaggt acagatgaag
    18181 aaagcggctg ggaaggtttg gactattatt ttaaaaaaga gcccaaacct ggtatggcct
    18241 ttagtcccga tgctcagtat ccggtcattc atgccgaaaa gggtatcttg atctttgatc
    18301 ttaactatga attggatatc gatgtagaag aagattacag taaatctact ttcaaatatg
    18361 aagttctttc tttaaagggt ggtaatgccc ctaatatggt tccagatgcc tgtgaggttg
    18421 ttatcaagac cgactttgag caggcctttg aacagaaatt taaaaacttc gtagataaaa
    18481 caaactatga atttgaatta gaaaagataa atagtaataa atataaagta accagcagtg
    18541 gagtttctgc ccacggcagt atgccggcca agggtgaaaa tgccattgcc agaatgatgg
    18601 ctttcctcgg tgagttagat aaagtaagta tcggagaatt tgttgatttc tataataaca
    18661 aaataggaat ggactattat ggagaaagga tcggctgtaa attaaccgat gaagtgtccg
    18721 gtaaacttat ctttaatgtc ggtcaaatca agttagaaga caatttagtt aaaaccactg
    18781 ttaatataag atatcccgtt accaaaagta aagaagaggt actggaaggg attaaaagtt
    18841 ctatcgataa gtatgaagcc tatcttgaaa tgaaaaacca ccaagaacct ctttatgtac
    18901 caaaagatga tccccttgtc aaggaactga tgaaggtata taaagaagtt acaggtgatg
    18961 aagatagtga accgatcgct atcgggggcg gtacttatgc tcgggcagta ccgaaggcag
    19021 ttgcctttgg cgctttattc cccggtcaag aagaattagc ccaccagaaa gatgaattta
    19081 ttggattaaa agacctttat aaaaatgcta aaattattgc ttatgcaatt aaagcattag
    19141 cttctaagga gtaggataat ttatggaagt aaaactcaat aactttcccg atataaaagt
    19201 aggtaactat accgaccagg aagctggtac aggctgtaca gttactcttt ttgataagcc
    19261 attggccggt tcggttagta taatgggggg tggaccaggc agtagggaat tagcactttt
    19321 agaccccact acccggatgg atcagattaa cgcagtctta cttaccggcg gcagtgcctt
    19381 tggtctttca gccgctgatg gtataatgaa atatctagag gaaaataata agggttttga
    19441 tactggattt gcaaaagtac ctattgtacc gggtggagtt atctttgatt taaactataa
    19501 gcataatgga atcagaccgg gtagtaatga aggctattat gccagtacaa atgctgataa
    19561 taaatttgaa attggttcca aaggtgtagg caccggagct acctgtggaa aactattagg
    19621 tgttgataaa gcctctaaat caggtctggg atattctgaa aacaattccg acccgataaa
    19681 agtctcagct ataacggttt gtaatgcggt cggcaatgta gttaacccta aaaccggtga
    19741 aattgtagcc ggtatcaaag gagaatctgg ttatatatcc tatcaggata ttttagagag
    19801 tgaaataatg gacctatttg gtactaatac caccctggga atagtcatca ctaatgtaaa
    19861 attaactaaa gcgatgctta aaaaagttgc tgaaatggcc caggatggat tggcacgaac
    19921 cgtaaaccca gttcatacca tgtttgacgg tgatatgata tttgcctttt ctaccaatga
    19981 ggtagaggca gaccttaata aaacgggaat tttagctgct caatccatat ctgatgcgat
    20041 cttaaatggt attgcagcag taaataatga aaaggaagga gaataaaaat gaagcatgta
    20101 aacactgatt ctttaataga atatctaaaa aatggacctt ctcccttcca tgttgtagat
    20161 aaaacagctg atatattaga aaacaatggt tatcaagagt taaaaataca aaacaaatgg
    20221 gatattaaag agaataagaa gtattatgtt ataaaaaatg attcagctat tattgctttt
    20281 aatactggaa gtaaagaaaa attaaaagac ggttttaaaa ttgttggggc ccatactgat
    20341 agtccttcta ttaaaataaa gccccaaccg gagtttaaag atgaagatta ttatctaaaa
    20401 ttaaatacgg aagtatatgg gggtccaatt ttgaacacct ggtttgatag gcccttaacc
    20461 ttagctggac gggtaagtgt tttagaagat ggacaactta ataatataaa tatcaatttt
    20521 gaagaccctt taatggttat tcccaatctg gctattcatc taaacagaga gataaataaa
    20581 gggcaagaaa ttgataaaca aaaagatctt atcccgatca tgaaaacaat caaggaagat
    20641 gaagagatag aagggtattt aatagatatg atagccagga gacttgaggt tgaaaatgat
    20701 aaaataatgg actttgacct ctatttaaga gataccgaag atcccattat catgggtagt
    20761 gaacaggaat ttttaaatgc cggccaaatt gatgatttgg gtatggccca tgccggttta
    20821 aaggccctat taaatactga gaattcaggc tttaagctgt tagtcctctt tgataatgag
    20881 gagatcggaa gtaataccga tgtaggggca gattctcccc tgttaagtaa tcttttagaa
    20941 agaattgtat atgccttaga tggtagtcga gaagattatc tgcagataat tgaaaatacc
    21001 tttatgatct cagctgatat ggcccatgcc gttcatccca actatgctga taaacatgat
    21061 cctactaaca aaccggtaat taatcaggga ccggttttaa agataagcag taataggaga
    21121 tatactaccg atagttacag cggcggctat attaaaaagt tatgtgcgga aaataatata
    21181 ccatatcaaa attttgtaaa ccactccaat gaaagaggtg gttctaccat aggacccctt
    21241 aatttaagac acttaggggt aaaatctgtc gatattggaa accctgtttt atcaatgcat
    21301 tcaattagag aactatatgg cttacaagac catgaatcaa tgatagaatt actgactgtt
    21361 ttttacaatg attaataaac tggaggtaca agaatggagt ataactttga taaaaagatc
    21421 gaccggacgg gaacagactc aataaaatgg gatatgaccg atcagtattt tgataaaaaa
    21481 gactgtctgc cgctctgggt ggctgattct gactgggaaa ccgcccctga gatcaaagaa
    21541 gcagtcagag acagggtaga acacggtatt tttggttata cgtatccctc agagaaatta
    21601 acccaaacta taatcaaatg ggttaaagaa cattataact ggcagataaa aaaagagtgg
    21661 atcgtttatg ccaacggggt agtaccttct ataaatattt cgatccgggc tttaacaata
    21721 cccggtgatg gggtaatcat ccagaccccg gtctatttcc cctttatttc aactatcaat
    21781 aatacgggta gacagattgt taataataaa ctaaataata atgatggtta ctatacaata
    21841 gattttgagg gactgcaaga aaatatatct ccggatagtg aagaaggatg taaaagagca
    21901 gagatgtttt tattctgtag cccacataac ccggtcggga gagtctggca gaccgaagaa
    21961 ttagaaaagt taaatcaaat tattatagat aatgatctga ttctggtttc cgatgaaatt
    22021 catgctgact ttgtcttcgc agataaccag catattccac cagctagcct ttctaaggag
    22081 attgaaaaca ggagtataac cctgatggcg cccagtaaga cctttaatat tgccggtctg
    22141 catacttctt ttgccgtaat tcccaataaa aagatcagaa agaagtttat gagtgtcaaa
    22201 gaccacttag taggggcggg taatatattc ggtctaactg ccatggaagc tgcctatagt
    22261 aaaggggagg actggttgga agctcaactg gattatctaa agggtaatat agattatacg
    22321 gctaattatc tgaaggaaaa tttaccccag attaagttcg ccaaaccaga aggaacttat
    22381 cttttttggc tcgattttag aaatataacc aactgttctg aagatattaa tgagaagtta
    22441 gttaaagagt tcagtgtagc cttagaacca ggtaactggt ttggagaagg aggggccggc
    22501 tttctgagaa tgaatctggc caccccaaga gagaatatta aaagggcttt agaaggtatc
    22561 aagaaatatg tagataacta tggaaaatag atggagtgaa aaaatgaatg atgtaatagt
    22621 aatcggcggc ggtccggccg gaatgatggc ggcaggcacg gccggtagta gagacaaaaa
    22681 ggttcaattg ttcgagaaaa atgagaaatt aggcaagaag ttatatataa ccggtaaagg
    22741 aagatgcaac tttaccaata aagcggatgt aaataccctt attgaaaatg ttgtaaataa
    22801 cagtagtttt ctttacagtg ctttttacag catggatagc gagagattaa ttaaattttt
    22861 tgaagaccta ggtttgaaaa ccaaggtaga aagaggtaat agggtctttc cccgttctga
    22921 taaagcaagt gatgttacca agaccttaaa aaagtactta aaagaaaaca atgtagaagt
    22981 aaagtataaa aaagtgcagg aaatattaac atccgataat caaataacag gtgtccaaac
    23041 taccgatggt aaggtgtaca attgtgaatc tgtcgtttta gctacggggg gatataccta
    23101 ccggtcgaca ggttccaccg gtgaaggtta tgaaatggca aaaaaactgg gtcacacggt
    23161 aaatgaactt tatccctcac tggttccttt agaacttcat gaaaactatg aaacaaaaaa
    23221 attagaaggc ttaagtttaa agcatgttga aattaaaatt agaaaacctg ataatcagat
    23281 aatatatcag gattttggtg atatggtatt tactgaacaa ggtgtttctg gaccaataat
    23341 tctttcggcc agttccaatt tgaaaaaggt aaaagataaa aattatatac tctccataga
    23401 tttaaaaccg gccttagact atgagacact gaatagaaga cttcagaggg attttaaaaa
    23461 atatgcaaat aaggatttta aaaatgcttt tgacgacctt ttaccttcca agctgatacc
    23521 aatatttcta aaaaggaccg gtattgattt aagaaagaag gtaaatcaaa ttaccgcaga
    23581 agaaagagaa aagattatta gtttactgaa agaatttaat tatacgatca aggattacag
    23641 ttcttttaac gagggtattg tgacagcagg gggagtagag gttaatgaaa tcaatccctc
    23701 tactatggaa tctaaaatag tgaagggact ttacctggcc ggtgaaatga ttgacgtcga
    23761 tgcttataca ggtggtttta atttacagat tgctttttca actggttatt tggccggttt
    23821 gaatgtctaa aaatgaaaat tatatatgta aaataaaaaa ggttcccttc gctgggaacc
    23881 ttaattacta attatattta aatttagtta aattacgtaa ttatattact tgaaccttgg
    23941 tcttgagcag cttcttgttc ttgctcttgt tcggttatgt atccattaat tattttgtta
    24001 acggagttca taacttcatt aaatttcttt tggctttcaa tataagactt aatggaagga
    24061 ttttgttcca ttttcatctg tagactctgc atttttttct gttgttggtc atttacttcc
    24121 tgaccattag cacgtgccat ttgcagctgc ttattaatat tttcatattc ttctaataaa
    24181 gaagtagcat cttcatcatc atataaagcc ttttcttttc tttttagttc tttatattcc
    24241 tctgattcaa caagttcttg acctaattct ttcgcttttt caattactga cataatttta
    24301 ttcacctctt attttgtagt tatcaacagt tattaatctt aacatcacag gccccatatg
    24361 tcaaataatt cacatctttt ttaagaataa tctagagttt aaaggtaaac tttgacccct
    24421 gatccaattc actttccact tctatttcac catcatgttt ttcaattata tccttaacaa
    24481 ttgcaagacc aagtccactt cctttttctt catctctggt ccgggcagtg tcaattttat
    24541 agaatctttc ccaaatattt tccagttctt cgactgctat accaacccca ttatcaataa
    24601 cagaaaattc aacctgataa ttggattggt cagcttcttc tacagtgatc gtaatcttac
    24661 tattcggatc tgaataattg accgcatttt caataagatt aataagaact tgatgaattt
    24721 tgttcttatc aatattaatt tcaggtagat cttcctcgat atttttaaca atttcgatat
    24781 ttttttcttc tattatactt tctagagatt ttaaggctcc atttaccaat attttagggg
    24841 aagtttgttt tttatctaag gattctttag cagtatccag tttagaaagt tctataagat
    24901 catttaataa tttttccagg tgttgagtat ccttaaacat gatatctaaa tattcggctc
    24961 gttcttcttt acttaaatcc tgttctttta atatttctaa aaatcccttg atagaagtaa
    25021 ggggagccct aaattcatgg gagacatctg ccacaaactg tttctgcatt ttctccagtg
    25081 ctctttgttt atctaaagtc ctattaatct gttttgcggc ataattatat gtattggcta
    25141 gatggttaat ttcttctgta ctgttttcag gcaggtctac tttttcgaca tttccttctg
    25201 tactttttaa agcagcttct ttaatcctat cgataggacg tgtaattctt cttgaaaaag
    25261 agatacttaa gaacacagcg gctgctaagg caactagaaa agagtacagg ataagtttca
    25321 taatattatt aatagttgca ccaatattac cgaggggtgt ttggattaat acagctccga
    25381 taatattatt tttctcagct cctggttggt taccagcgat tttaacatct tcctcatcgg
    25441 tttgaaacaa ggggacagcc atcaaaagat gccgatagtc gggtcccata atttttttag
    25501 taaaggtatt accggatagt acctggttta attcttcttg ttctaaagac aaattcgcct
    25561 ctaaggaagg aacatttaag accatttcgc cggttttatc aattaatcca atatccatat
    25621 tagtggaacg ggcaattgta ttgattttag acctggtact tccaatacta aaatcatctg
    25681 ttaaaagaat attaccttct ccaatatttt cactgactaa agtagcaatc cttctgctgt
    25741 tattagttgc ttcccattct tttaagccgt tatagtaatt ctgaactaaa tagccgaaca
    25801 ttataccaat tacagctaag gaaataaaga taacgattaa ataagaaacc ataagacgac
    25861 ccaatatgct gttaatattg aagagctttt taatcattat tattttcctt tcgtatggaa
    25921 aatttataac ctacacccca gactgtttta atgggttgga actcaattcc cgtgttattc
    25981 aatttttttc ttatcctttt aatatgttca tcaatagttc ttgtttcagt taagttttca
    26041 aaaccccaaa tattttctaa aagctgttcc cgcgtgaaaa cctgttcttt attttgggct
    26101 agaaaggaga gtagcttgaa ctctttggga gtaaaagata cattttcctc atcaatggta
    26161 acggtgtaac tatcggtgtc tatttccata tttaaactat caaaataaag ggtatctgtt
    26221 ttcttagact ctgttttttc atagcgcctg aggactgctt tgatacgggc agttaattca
    26281 cgagggctaa aaggtttggt tacataatca tcggctccaa tctcaagtcc cagtacttta
    26341 tctgcttctt cacttttggc agtcaacata attatcggga gatcattctt tttttctttc
    26401 aattctttac agatctccca accgtccttt ttaggtaaca tgatatctaa aagcagcagg
    26461 tcatattcat tttgcagagc ttcttttaaa ccctctttac catttttctt taagattaaa
    26521 gagttattat catactgttt aaacatggct ttgataacag caaagatatg ctcatcatct
    26581 tcaattacta gaatcttttt attatataaa tccatattat ctccccatat tttatatact
    26641 gttattttat catttcaata tttaaattaa cttcccttta tattttacaa tttttttaca
    26701 gcttctttat agaaatttga taataatatt atttaatatt attaaagaaa ggagtataat
    26761 aagaatagaa gatagggaaa ggggggatca tcatgatttt tggcaaaaat tataatgatg
    26821 aacttttaca gaaagaagca aagaatgcga ttgaagagca gcccaccttg cgaggaattc
    26881 ccaatattaa tgtcacctca gataatggaa tagtaagtat ttttggttcg gtcagaacgg
    26941 aagagttaaa aaaggacata aaaaagataa ttgaaaataa atatgatgaa aaaaatatag
    27001 attacaaaga aatacaaaat gatcttacca tataaatatt tttaaaggca accgttctgg
    27061 ttgccttttt tatatattac ttcatattct tttgtaattc ttcatggctg acactgcgga
    27121 aatgttcagt cggcagccaa ctcttattat ttaaagtaaa gagggcatag tagagaatta
    27181 aaacccagct gtacataaag atcgggaaga atatgacata tttaaaggaa tcaaaagata
    27241 agttatccaa taagtataca gatgatggta ttataaaggg cattatgggt aagataaagc
    27301 ccagagtttt aatgttttga ataaagagat taaacagggg tgaattaaaa gtccctggaa
    27361 atgtcagtct aagtatagtt actacaaaat aagtcattag aaaaggttgt tgtattaatc
    27421 tcatggctga atcaatcttt acaatattta attcttttat tccctgcatt aacatctttg
    27481 gtatatactt aaataacacc gatagctggc cgcgccccca gcgtaatctc tgacggcagc
    27541 ttgcataaaa ggagacaggt ttttcatcga atatttttgt ttcgcgggca aaagtagttt
    27601 tagtatcttt aagtaaagcc tggattgagt actcaagatc ctctgttaaa gtaaccgtat
    27661 cccagccaat tttacttaag gattcctggg agatacacat acctgtaccc atcaggacac
    27721 tggaaagccc tacattaaat cttgatagta gattatagcg gtcattgatc caaaacatca
    27781 tggaaaaggt ggcggttacc cagttatcag ccggattctt agaatctata taggcctgaa
    27841 taatcttttc tccctgcaat agtcgggtgt tcattgcctt taagaagttc tcttgtacca
    27901 aattatctgc atcgaaaacc acggctgcat cataatctgt ttttccattt aaaaatccta
    27961 aactgcgaaa ggcatatctt agtgcatatc ccttaccttt cttttcttga ttaaatctgg
    28021 acaggacatt aacaccttgt tctttagctt tttgagctgt atcatcttca cagttgtcag
    28081 ctaccacata tatgtcataa agatctttcg gataatttaa gttgcgtaag ttttcaatca
    28141 atttatctat taccttttct tcattatggg ccggtatgac taccgcaaac ctattggccg
    28201 gttcttttaa gtcattattc ttt
//
LOCUS       DATGCJ010000034         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_495305, whole genome shotgun sequence.
ACCESSION   DATGCJ010000034 DATGCJ010000000
VERSION     DATGCJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495305"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VKN64_01555"
     CDS             <1..1083
                     /locus_tag="VKN64_01555"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMA58947.1"
                     /translation="GEVIDFEIIGADKTLLKIELDNILKPKERLKIIMEYTLKIPNLE
                     HRFGYYKSTFNFGNWYPIAAVYDQKGWNLSEYYVIGDPFYSDIGNYNISIEVPSNYII
                     AASGKILSEEIFEDKKIWKIRARLMRDFAWVASEDFTKRTHNLKDTQVKIYFLTDNKD
                     LMNYTKNIARQAMQIYSKKFGKYPYGQVSIVETNFLSGGMEYPGLVYIDTDKFIGSQK
                     TYLSEYIVHEIAHQWWYSTVGNNQITDPWLDEGLATYSQIVFSRETRGEAYAEGLYRS
                     LMGIYNTSRSNRDMLTPVTEFTAWNDYSAVAYGKGAAFLHDLEQKYGTEDVEKILQEY
                     YKRFKFKNAFSEDFFKIVEEVVGAQY"
     gene            1103..1624
                     /locus_tag="VKN64_01560"
     CDS             1103..1624
                     /locus_tag="VKN64_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA58948.1"
                     /translation="MIKKAKIEDLKEVFNIYQICRKELEKNNILMWDDNYPTRDLIQK
                     EIEAGTIFLIKKRDKIICSFVLSDYLDDLWEPIGWQDNNFLGLHLLAVHPDYQRQGYG
                     EKIVKFSENYAKVNNYSSIHLDVLSKNEAALNLYKSKGYKKVGELFFDFKPAGFQEYH
                     CYEKILCAKDEII"
     gene            1621..2259
                     /locus_tag="VKN64_01565"
     CDS             1621..2259
                     /locus_tag="VKN64_01565"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMA58949.1"
                     /translation="MSLAINLLSKVLILVLFYFSWQNLEQVGLKLNQNLVYFYLIMVV
                     LSLIVDKFFFKGQASENKKEDKVLSTLLSVSWFSALIIPILEYSYFIRSNSVISTFGI
                     IVVFIGIIIRGLSIKYLGKYFSRDVETWHNHTLVRSGLYKYIRHPAYLGNILQIIGFP
                     LILNSYYSLVLSVITVYLFLKRIEVEERFLEKELEKYKEYQAETKKLIPYLW"
     gene            2297..2794
                     /locus_tag="VKN64_01570"
     CDS             2297..2794
                     /locus_tag="VKN64_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58950.1"
                     /translation="MKNLSIVSLKIMAIYFLVTFIRRGLLSGLYMIFTNQMPLLSKIG
                     LILSELIFLIIIYFLWFKAEEIAGIITRHLDIENKVAKEVEYDLLFQTLLALAGVIII
                     TISLPDFIRYIFEILSVAHISMFKETPRFMSKIAEILLGLFLVYRAQKISHLIFKQER
                     NSNGI"
     gene            2784..3263
                     /locus_tag="VKN64_01575"
     CDS             2784..3263
                     /locus_tag="VKN64_01575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA58951.1"
                     /translation="MEYRFKPMQIEDAKKVKNWQYNGFIQKIFMEPYFKSYQKGDDIL
                     KGPNNCKGFAVYKDNDLFGLFEYYFNSSLMEIGLALNPKFVGSGMALEYLIEGLNFGI
                     EYFNYQEELIKLNVNKENKPALKVYKKAGFQITNIEKEEFEMQIETKNISKKSSNNE"
     gene            3256..>3466
                     /locus_tag="VKN64_01580"
     CDS             3256..>3466
                     /locus_tag="VKN64_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58952.1"
                     /translation="MNNNFFCSWSGGKDSALSLYKAIQKDLKPKKLLNMMNEKGENSR
                     SHNLPLSILKAQAESLNIPLTTKRSS"
BASE COUNT         1260 a          442 c          636 g         1128 t
ORIGIN      
        1 ggtgaagtaa ttgattttga aattatcggt gcggataaaa ccttgcttaa aattgaatta
       61 gataatattt taaagcccaa agaaagatta aaaataataa tggaatatac tttaaagatc
      121 cccaatttag aacatagatt tggttattat aaaagcactt ttaatttcgg caactggtat
      181 cctattgcag ctgtctatga tcaaaaaggc tggaatctat cagagtatta tgtaattggt
      241 gatccttttt atagtgatat tggtaattat aatataagta ttgaagtacc ctctaattat
      301 ataattgctg cttcaggcaa aatattatca gaagaaattt ttgaagataa aaaaatatgg
      361 aagataaggg ccagattgat gcgcgatttt gcctgggtgg ctagtgagga ctttactaaa
      421 agaactcata acctaaaaga tactcaagtt aagatttact ttttaactga taataaggat
      481 ttgatgaact ataccaaaaa tattgccagg caagcgatgc aaatttatag taaaaaattt
      541 gggaaatatc cttatggtca agtatcgata gttgaaacga actttttaag cggtgggatg
      601 gaatatccgg gactggtcta tattgatact gataaattca ttggttccca aaagacttat
      661 ttaagtgaat acatcgtcca tgaaattgcc catcagtggt ggtattcgac agttggcaat
      721 aaccagatca ctgatccctg gttggatgaa ggacttgcca cctattccca aatagttttc
      781 agccgtgaaa caaggggaga ggcatatgca gaaggtttat acaggagttt aatgggcatt
      841 tataatactt cccgcagtaa tagagatatg cttacaccag tgacggagtt tacagcctgg
      901 aatgactata gtgcagtcgc ctatggaaag ggagcagctt ttctacatga tttggaacaa
      961 aagtacggga ctgaagatgt agagaagata ctgcaggaat attataaaag atttaaattc
     1021 aaaaatgctt tttcagaaga tttcttcaag attgttgaag aagtggtagg ggctcaatat
     1081 taagaggaaa ccggtgatta aaatgatcaa aaaagcaaag atagaggatt taaaagaggt
     1141 atttaacata tatcaaatat gcagaaaaga attagagaaa aataatattt taatgtggga
     1201 tgataattat ccaactcgcg atttaattca aaaagaaatc gaagccggta ctatattttt
     1261 aataaaaaaa cgggataaga taatatgttc cttcgtctta agtgattatc tcgatgattt
     1321 atgggagccc ataggctggc aggataataa cttcctgggc ttacatctct tagctgttca
     1381 tcctgattac caaagacagg gttatggtga aaagattgtt aaatttagtg agaactatgc
     1441 taaagtaaat aattattcca gtatacatct ggatgtatta agtaaaaatg aagcagcctt
     1501 gaatctttat aaaagtaagg ggtataaaaa agttggtgaa ctgtttttcg actttaaacc
     1561 ggctggtttt caagaatatc attgctatga aaaaatatta tgtgcaaagg atgagataat
     1621 ttgagtcttg caattaattt gttatcaaag gttttaatat tagttttatt ctatttttcc
     1681 tggcagaatc tagaacaggt cggattaaag cttaatcaaa atttagtata tttttatcta
     1741 atcatggtag tacttagtct gattgtagat aaatttttct ttaaaggaca agcgagtgaa
     1801 aacaaaaagg aagataaagt tttaagtact ttactttcag taagctggtt tagcgcgtta
     1861 attataccta tattagagta ttcatatttt attaggagta atagtgttat ctccaccttc
     1921 ggtattatag ttgtttttat tgggataatc attaggggct taagtattaa atatttgggc
     1981 aagtattttt cccgggatgt agaaacctgg cacaatcata ctttggtcag gtcaggactt
     2041 tataaatata ttagacatcc cgcttatttg ggtaatatct tacagataat agggtttccg
     2101 ctcatcttaa attcatatta ttccttggtc ttatcagtaa taactgttta cctattttta
     2161 aaaagaattg aagttgaaga gagattctta gaaaaagaac tggaaaagta taaggaatac
     2221 caagcagaaa ccaaaaaatt aatcccttat ctttggtaga taatatttta aatacaaaac
     2281 aaaggggaga tcaacattga aaaatttgag tatagtttcc ttgaaaatta tggctatcta
     2341 ttttttggtt acttttatta ggagaggttt actttctggg ttgtatatga tctttactaa
     2401 tcaaatgccg ctattatcaa agataggttt aatcctgtct gagttaatat ttttaatcat
     2461 aatatacttt ctctggttta aagctgaaga aatcgccggt ataattaccc gccatcttga
     2521 tatagaaaac aaggtggcta aagaagtaga atatgactta ttatttcaaa ctctcttagc
     2581 tctagcagga gtaatcataa ttactatctc actaccagat ttcattagat acatatttga
     2641 aatactatcc gtagcccata tatcaatgtt taaagaaact ccccggttca tgtctaaaat
     2701 tgccgagata ttattaggat tatttttagt atatagagct caaaagatat ctcatctaat
     2761 atttaaacaa gaaaggaata gtaatggaat ataggtttaa accaatgcag attgaagatg
     2821 ctaaaaaagt taagaactgg cagtataatg gttttattca aaaaatcttt atggaaccat
     2881 attttaaaag ttatcaaaaa ggtgatgata ttttaaaagg gcccaataac tgtaaaggtt
     2941 ttgcagtcta taaggataat gatttatttg gcttatttga atactatttt aactcaagtt
     3001 taatggaaat tggactggct ttaaatccta aatttgtggg cagtggtatg gcgttagaat
     3061 atttaataga aggattaaat tttggtattg aatatttcaa ttatcaagag gagttaataa
     3121 aactaaatgt caacaaagag aataaacctg ctttaaaggt atataagaaa gcaggttttc
     3181 aaataactaa tatagagaaa gaagaatttg aaatgcagat agaaacaaaa aatatttcaa
     3241 agaagagctc aaataatgaa taataatttc ttttgttcct ggagtggtgg taaggattct
     3301 gcactttcat tatataaagc aatacaaaaa gatttaaaac ccaaaaagct gttaaatatg
     3361 atgaatgaga agggtgaaaa ttcaagatcc cataatttac ctttatctat tttaaaagca
     3421 caggccgaat ctttaaatat tcctttaaca acaaaaagat caagtt
//
LOCUS       DATGCJ010000035         5078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_226866, whole genome shotgun sequence.
ACCESSION   DATGCJ010000035 DATGCJ010000000
VERSION     DATGCJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226866"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VKN64_01585"
     CDS             <1..288
                     /locus_tag="VKN64_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58953.1"
                     /translation="LSPLATQQKMIEELKETLDSLKGISLINKLNAEDLNEQERSKFF
                     KEVLIQICDLDICSSLFMTSMAENFDFDISRLEEHEINRMKQKGYMSRGLY"
     gene            430..588
                     /locus_tag="VKN64_01590"
     CDS             430..588
                     /locus_tag="VKN64_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58954.1"
                     /translation="MIKYMDLAARIVIGFGYLLVGYVIWKEGKKIIAERKKDNTKEKT
                     QLDEQLSS"
     gene            635..1588
                     /locus_tag="VKN64_01595"
     CDS             635..1588
                     /locus_tag="VKN64_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HMA58955.1"
                     /translation="MQAKKLFNTKNMTDQEWLEKRNSLGIGGSDVSAITGINPFKSAV
                     EVWLDKTGRSNEKEDNNAMYWGRELEDKVADEFAKRRSRELDQEVKVWKENHILQHPK
                     YEFMIADIDRRVVGEKALLECKTTVDFNKLWSEKEFPDMYMLQVQHYLAVTGYKKAYL
                     AVLIMNRRKFYYYTIKRDEELIDLIIENEKDFWNNYVKKDVPPEVDGSKASSRLLKRM
                     YPDADSSKKIDFEKEDLDLIEKRNEYHEIEKKYKKKRKKCENKIKNKLQEAEAGVYQG
                     EEVVKWKNISSRRVKTKKFREDHPELYNEYSYQSNYRRLYI"
     gene            1685..2470
                     /locus_tag="VKN64_01600"
     CDS             1685..2470
                     /locus_tag="VKN64_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HMA58956.1"
                     /translation="MSKTKQSELKKQLENNTKKDKKKPEPIQKKVYNYLEKMKPEIER
                     ALPKHIGAERMARIAYTTIRTTPKLLKCSMGSLMGAVMQAAALGLEPNMLGQCYIIPY
                     GNEATFIIGYRGMINLARRSGEIKTIYAHVVKENDEFEYEYGLEPMLKHKPASSDRGE
                     IIKTYMVAKFNDGGYYFEVMDIEDVLKRKAKSKAAKSKFSPWNDELGYEEMVKKTVIR
                     HGFKYLPVSIEIMNTVETLDESQKDIEEMEENEFIDIDIKQSA"
     gene            2534..3364
                     /locus_tag="VKN64_01605"
     CDS             2534..3364
                     /locus_tag="VKN64_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HMA58957.1"
                     /translation="MGNGRYIKIVTKIWVDEKIRDLNDQGQKLFIYILTSPHSNMAGY
                     YRLPKPYIHYDLKWSSEQLSKPFNKLLNNKLIKYCEESSVVLIPRFLKYNSIQNQNQA
                     KGAAKKTSELPKNSLVPYYKKICKTHFKQYYELLIKGLPKPLGNTETEAEVLIEEETE
                     TVKETEKIDHGEQEFEIKNFNQGLDDLLSPFSNYLHQSIEKDHLKIMNSYLNKGMDVM
                     VLIEAMKRSEGKKVPFKYFIKILHTWEKENIKSLDDIKRLDVKFSQKKFSNTKKWKYN
                     "
     gene            3766..4017
                     /locus_tag="VKN64_01610"
     CDS             3766..4017
                     /locus_tag="VKN64_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58958.1"
                     /translation="MGLKKAKTICKLQGISFNLEVVILCPYCGNKSMRLIIRENTAEC
                     YRCNKKQSIDDILEITKSDWSEKHKKYEKLMRFNRGVDY"
     gene            4017..4463
                     /locus_tag="VKN64_01615"
     CDS             4017..4463
                     /locus_tag="VKN64_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58959.1"
                     /translation="MEEIFSRIISALVHYKEYKQRLKIIDTKLKSLSDRPLRKSINKT
                     AVQTSNRKDYTAEIAVDRLEGKLKKEYKSKQMFIKKVEIAREGLDPVEKIIIDEKYLK
                     GRILPDVDIYTHPKFIYGKTKYYEIKSNALTKLGRIMGINNKKSKK"
     gene            4548..5033
                     /locus_tag="VKN64_01620"
     CDS             4548..5033
                     /locus_tag="VKN64_01620"
                     /inference="COORDINATES: protein motif:HMM:NF027987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HMA58960.1"
                     /translation="MQCQAIKNNGEQCNRKVKKGQQYCWQHKDQAVQGGRPKIYNSIK
                     QMQKDIDKYFKSCIKKIIDPETGEITVEQIRPFTITGLALALNMDRRTLLNYEKDDKF
                     FPTIKKAKLKCENFAEEQLFKGGTVSGVIFNLKNNYNWKDKQEIDHKGDIEIDVTLED
                     E"
BASE COUNT         2189 a          657 c          987 g         1245 t
ORIGIN      
        1 ttgagtcctc ttgccactca gcagaaaatg attgaggagt taaaagaaac tttagattct
       61 ctaaaaggaa taagtttaat taacaaatta aacgcagagg acctcaatga acaggaaaga
      121 agcaaatttt ttaaagaagt actaatacag atttgcgatc tggatatctg cagcagctta
      181 tttatgactt cgatggcaga aaactttgat tttgatatca gccggctgga agaacacgag
      241 attaacagga tgaaacaaaa aggatatatg tcaagggggt tatactaatc gataataaaa
      301 ttaaaacatt ttgtaaagac tgtaaatatg atcctgagac gtgcggaaaa aacccaatag
      361 aatgtatgaa aactgaagaa tcaagattat atttcagaat ctatgataaa gggctggggt
      421 ggacaacaag tgattaaata catggattta gcagcaagaa tagtaatagg atttggatat
      481 ttattagtcg ggtatgtgat ctggaaagaa ggaaaaaaga taatagctga gaggaaaaag
      541 gataacacaa aagaaaaaac tcagctggat gaacagctga gtagttaaat ataaaattca
      601 actattaaga attataacat caggaggtaa taaaatgcaa gcaaaaaagt tatttaatac
      661 aaagaatatg acagatcaag agtggttaga aaaaagaaac agtctaggaa ttggtggtag
      721 tgatgtatct gcaataacag ggattaatcc ctttaaatca gctgtagaag tatggctgga
      781 taaaacagga aggtctaatg agaaagaaga taataatgcg atgtactggg gcagggagct
      841 agaagataag gtggctgatg aatttgctaa aaggagaagc agagagttag atcaagaagt
      901 taaagtctgg aaggaaaacc atatactgca gcatccaaag tatgaattca tgatagcaga
      961 tattgacaga agagtagtcg gagaaaaagc acttttagag tgtaaaacaa ctgttgattt
     1021 caataaatta tggagcgaaa aagagtttcc tgatatgtat atgctccagg ttcagcatta
     1081 tcttgcagtg accggttata aaaaggcata cttagcggta ttgataatga atagaaggaa
     1141 gttttactac tacacaatta aaagagatga agagttgatt gatctaatta tagaaaacga
     1201 aaaagatttc tggaataatt atgttaaaaa agatgtaccg cctgaagtag acggatcaaa
     1261 agcgtccagc cggctgttaa aaagaatgta tcccgatgct gacagcagta aaaaaataga
     1321 ttttgaaaaa gaggatctgg atctaataga aaagagaaat gaatatcatg aaatagaaaa
     1381 aaagtataag aaaaaaagga aaaagtgtga aaataaaata aaaaataagt tacaggaagc
     1441 agaagccggg gtataccagg gcgaagaagt tgtaaaatgg aaaaatattt cgagtagaag
     1501 ggttaaaaca aaaaaattta gagaagatca cccagaactc tacaatgaat attcatatca
     1561 gtcaaactac aggaggttat atatataatg aaatttgaag agtacttcga aaaattagaa
     1621 tgtgagtatg gtaaaaatat caagaaaaca aatgtaacac aaaataaaaa ggaggatcaa
     1681 gataatgagt aaaactaaac agagtgaatt aaaaaaacaa cttgaaaata atactaaaaa
     1741 ggacaaaaaa aagccggagc caattcagaa aaaagtttac aactacttag aaaaaatgaa
     1801 acctgagata gaaagggcac tgccgaaaca tataggagca gaaagaatgg ccaggatcgc
     1861 gtataccact atcaggacca cgcccaaatt attaaaatgt tctatgggat cactaatggg
     1921 ggccgtcatg caggcggctg ccctcggcct cgaacccaat atgctgggcc agtgttacat
     1981 aataccttac ggtaatgaag ccaccttcat aataggatat aggggaatga taaacttggc
     2041 cagaaggtcc ggcgagatca aaacaatata tgctcatgta gtaaaagaaa atgatgaatt
     2101 cgaatatgaa tacggattag aaccgatgtt aaaacataaa cctgcttcat cggaccgcgg
     2161 agaaattatc aaaacctata tggtagcaaa gtttaatgac gggggctact attttgaggt
     2221 aatggatatc gaagatgtat taaagagaaa agcaaaatca aaagcggcaa agtctaaatt
     2281 tagtccctgg aatgatgaat taggatatga agagatggtc aaaaagactg ttatcaggca
     2341 cggattcaaa tacctgccgg taagtataga gataatgaat actgtagaaa ccttagatga
     2401 aagccaaaaa gatattgaag agatggaaga aaatgaattc atcgatattg atattaaaca
     2461 gtcagcataa aaaaccatat aaaagggcgt tttaacaagc gcccttataa cttaattctg
     2521 ggaggtgcag ttaatgggca acggaagata tataaagatc gttacaaaaa tatgggtcga
     2581 tgagaaaata agagatctta atgatcaggg gcagaaacta tttatctata tattgacctc
     2641 acctcacagt aatatggccg gttattacag gcttccaaaa ccttatatac attatgatct
     2701 taaatggtca tcagaacagt tgagtaaacc ctttaataaa ctgttaaata ataaattaat
     2761 taaatactgt gaagaaagtt cagtagtttt aattcccaga ttcttaaaat ataactcgat
     2821 acaaaaccaa aatcaggcaa aaggggcagc aaaaaaaaca tcggaactac ctaaaaacag
     2881 cttagttccc tattataaga agatttgtaa aacccatttc aaacagtatt atgaactctt
     2941 aattaagggg ttacccaaac ccttaggcaa tacagaaaca gaagcagaag tattaataga
     3001 agaagaaaca gaaacagtaa aagaaacaga aaaaatagat catggtgaac aagaattcga
     3061 aattaaaaat tttaaccagg gtcttgatga tctattatca ccattttcta attatttaca
     3121 ccaatctata gaaaaagacc atctcaaaat aatgaattca tatcttaata aaggaatgga
     3181 tgtaatggta ttgatagaag cgatgaagag atctgaagga aagaaggtcc catttaaata
     3241 ttttataaag atacttcata cctgggaaaa agaaaacata aaatcactgg acgacattaa
     3301 aagattggat gttaaattta gtcaaaaaaa atttagcaac acaaaaaagt ggaaatataa
     3361 ttaaaaggag aattcaaatg atttcaaaaa tagcagatat caaagacacg tttgactgtg
     3421 acaaagagac tgcagaaaat ataagagagt atgtaaaaaa aatgtataaa aaatattatg
     3481 agcaatataa caaagaacgg aaggagggtt aacccctcct tttaaactcc agataaatat
     3541 tatttcaata aagaatatta aatatgagac catcatataa cgacctcaaa ggcggtttct
     3601 gccggagagg ttaattagtg attacttaat ctcaagtgag taaataagct taaaatttat
     3661 aagaaaaccc agcctggata taaataatta ttttacctta atcgggatgt tttatttttt
     3721 tattaaaaat aatcattaga atctatagcc ataggagctg ataaaatggg tctcaagaaa
     3781 gcaaagacga tctgtaaatt acagggaata agtttcaatc tagaagtagt aatcctttgc
     3841 ccgtactgtg gtaataaatc aatgaggctt ataataaggg aaaatactgc agaatgctat
     3901 cgctgtaata aaaaacaatc cattgatgat atattagaaa taacaaaaag cgattggtct
     3961 gaaaaacata aaaagtatga aaaattaatg agatttaaca gaggggtgga ttattaatgg
     4021 aagaaatatt ctctagaata atctctgccc ttgtacatta taaagagtat aaacagagat
     4081 taaagataat tgataccaaa ttaaaaagtt taagtgacag gccattaaga aaaagtatta
     4141 ataaaacagc ggtacagact tcaaatagaa aagattatac ggcagaaatt gctgtagata
     4201 gactggaagg aaaattaaaa aaagaatata aaagcaaaca aatgtttatc aaaaaagtgg
     4261 aaatagcaag agagggacta gacccagtag aaaagattat tatcgatgag aagtatttaa
     4321 aggggaggat actaccggat gtagatatat atactcaccc taaatttatc tacgggaaaa
     4381 caaagtacta tgaaataaaa agtaatgcct taacaaaatt aggaagaatt atgggtatta
     4441 ataataaaaa aagcaaaaaa tgaactttta atgaacaaga agcagtatat taagtgttat
     4501 tataattata gttaaaataa ttattaaaac aaaatgagga attactaatg cagtgccaag
     4561 caataaaaaa caacggagaa caatgtaata gaaaagtaaa aaaaggacaa caatactgct
     4621 ggcagcataa ggatcaggct gtacagggag gaaggcccaa aatatataac tcaataaaac
     4681 aaatgcagaa agacatagat aaatacttta aatcttgcat aaagaaaata atcgatccag
     4741 aaaccggaga gataacagtt gaacagataa ggccgttcac gataactgga ttagctttag
     4801 cactaaacat ggaccgcagg accttactta actatgaaaa ggatgataag ttttttccca
     4861 ctataaaaaa agccaaacta aaatgtgaaa attttgctga agagcagtta tttaaagggg
     4921 gcactgtatc cggagtaatt tttaacttaa aaaacaacta taactggaaa gataagcagg
     4981 aaatagatca taaaggagat atagaaatag atgtaaccct agaggatgaa tagtattgcc
     5041 cagtattaag ctgaatatat ccaaaaaagt attcaaca
//
LOCUS       DATGCJ010000036        16381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_48762, whole genome shotgun sequence.
ACCESSION   DATGCJ010000036 DATGCJ010000000
VERSION     DATGCJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48762"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VKN64_01625"
     CDS             <1..887
                     /locus_tag="VKN64_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HMA58961.1"
                     /translation="YIIAGSITGRVKALLDEHGERIDEAGPATPVIVLGFDDVPEAGD
                     YVRVMEDEKRARDLAERRQEKEKEERLKKDSKVSLEDLYRNIQEGEIKELNVIIKADV
                     QGSIAALENSLMNIESDEVSVNIIHTGVGAINETDVNLASASNAIIIGFNVRPGTNAR
                     KAAEKEKVEIKTYRVIYKVIEDIKNAMSGLLEPEYKEIVVGRVEVRDTFKVPDVGIVA
                     GAYVKEGEVNRNDYVRLIRNGTIIHEGEISSLKRFEDDVREVKEGYECGIGIENYNDI
                     KVGDILEIYRYKEIERSL"
     gene            899..1279
                     /gene="rbfA"
                     /locus_tag="VKN64_01630"
     CDS             899..1279
                     /gene="rbfA"
                     /locus_tag="VKN64_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMA58962.1"
                     /translation="MANEKRVRRMNQLLKEIISKIILRELKDPRIGFASVTKIDVSND
                     LKHAKVMVSVLGSEQEKKETMEGLDQARNYIQKLVGERLTTYHTPKIIFRYDNSIEQG
                     VHISKLIDEVIEEDEKRHEDGEEE"
     gene            1263..2252
                     /locus_tag="VKN64_01635"
     CDS             1263..2252
                     /locus_tag="VKN64_01635"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HMA58963.1"
                     /translation="MVKKNNSLQEIVEYIKENDDYLIIGHIDPDGDAIGSIMAFKFLL
                     NHLNKNSLLLLHSDLTKEYDILFKYLNSDEYYIFNKGNEEQLKEYNKVIALDSANIER
                     LGDYKPIIENKQIVNIDHHYDNSRFGDLNYVNPDESAVGKIIYDMVDLLDIEITEDLG
                     TAMALAVLADTGSLKYPNSDPASFRMMASLKEEGIDIVKINRFLQSFSSLKYLKLLSE
                     ALANIKTAKQGKIAWLVVNNHTIKSIGIDEKEINNLVNYPRDIRGVEVGISFVETKKD
                     FIDISFRSLNYVDVSKIAHKFNGGGHPRAAGTKIKGALEQTVRAVLDEVKKNV"
     gene            2245..3168
                     /gene="truB"
                     /locus_tag="VKN64_01640"
     CDS             2245..3168
                     /gene="truB"
                     /locus_tag="VKN64_01640"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMA58964.1"
                     /translation="MSNQPGIINVLKPPGMTSFDVVGYIKKILQIKKAGHTGTLDPAA
                     AGVLPVCLHRGTKIIPYLPEEKKVYIARISLGLKTDTLDADGEILEKDNNWQSLDDNQ
                     IKEAVQSFSGEIMQKPPIYSAVKVNGKRLYHYARNDQEAKVEIEDRVVNIYGIEVLNI
                     NLPLIKIKVSCSKGTYIRSLARDIGSKLNTFAHLKNLLRTASGPFKLEDTVTLYEITN
                     ENLDNILISLDSPFDYRPMEVKDYAYKFAINGTKLLIKNFIKWPSDITIGERLLIYGK
                     GQFISISEVKEDEDEKIYIQPLRVFHDGGQN"
     gene            3171..4091
                     /locus_tag="VKN64_01645"
     CDS             3171..4091
                     /locus_tag="VKN64_01645"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMA58965.1"
                     /translation="MNIIRSNTFDQYEIDENIIAIGSFDGIHKGHKKILNKTLTLAEK
                     NNLKSGVLTFEPHPQEVVADEDNHYYITSLAQKRKILNEMGFDYNFEMNFTKDIAQTP
                     FDDFVKKIIVDNLNTKHIVVGTDFTMGYKGDGNIDELIKLGKKYGFDVTAVEPLKIGD
                     HKISSSVIRDLIMGGRLKELKEHLDRNFILEGEVVKGYGRGKKLGIPTANIKPATKYI
                     LPPDGVYAVYVYLNEDKYKGVANFGHKPTFDDDDFTIEVHLPDFQGDIYGEFVEIELI
                     EYLRGEQEFDDVDELIETIKQDILYIKEVL"
     gene            4191..4457
                     /gene="rpsO"
                     /locus_tag="VKN64_01650"
     CDS             4191..4457
                     /gene="rpsO"
                     /locus_tag="VKN64_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMA58966.1"
                     /translation="MISKERKQEIVQKYQRDEKDTGSPEVQIAILTERISQLTDHLKD
                     HKQDHSSRRGLLKMVGQRRKLLRYLENKDVERYRELISSLGIRG"
     gene            4551..6659
                     /gene="pnp"
                     /locus_tag="VKN64_01655"
     CDS             4551..6659
                     /gene="pnp"
                     /locus_tag="VKN64_01655"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMA58967.1"
                     /translation="MTNTWKTDFAGTELEFETGKVAKQANGAVTVKYGDSMVLVTATM
                     SEPREGINYLPLMVNYEERVYSIGKIPGSVNRREGRPRDFATLTARLIDRPLRPLFPD
                     GMRHDIQVITTVLSVDKDHEPDIIAMNGASAALTLSDIPFDGPIGGVKVGLVDGELVL
                     NPTEEERKNSKLDLTVAGTEEAVSMVEAGADEVEEEVILDAIDLAHEEIKKIVRFQKK
                     MQKEAGLEKQDISLVNNVDEKIEKLISDFATNRLREAINTVKKQERSEQIDELKEDAK
                     EYLAGKLEDYEEKEEEYNKMVEAAFDDLTKKLVRKMIIEDGKRPDGRSYDDIRPIWCE
                     VGNLPRAHGSAIFTRGETQALSVVTLGASSDEKTIFGLGEDETKRYMHHYNFPPYSVG
                     ETNPLRSPGRREIGHGHLGERALKPMIPSKEEFPYTIRVVSEVLESNGSSSQASICGS
                     TLSLMDAGVKIKQPVSGIAMGLIKEGDDYAVLSDIQGMEDHYGDMDFKVAGTPNGITA
                     LQMDIKIGGLSKDILRNALNKARKGRLYILDKMLEVIDEPRENLSEHAPLIMTMQIDP
                     EKIRHVIGPGGKMINKIIDETDARIDIEDDGTIFILAEGQENGQKAKKMIDDLTREVE
                     VGEVYEGKVVKIMNFGAFVEILPGKEGLVHISNIADRHINEVSDVLSEGDEIRVKVIK
                     IDNKDRINLSMKDVEQDFEV"
     gene            6818..7657
                     /locus_tag="VKN64_01660"
     CDS             6818..7657
                     /locus_tag="VKN64_01660"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HMA58968.1"
                     /translation="MKLGRHLSISGGIDKAIDETIAINANSVQIFTKSPRSWQKKEIS
                     EEEIKKTREKIKKYEIKAFVVHSSYLVNLATPNDDLYYKSKEEIKEDYRQAGRLGADY
                     YVFHPGNYVKSSKEAGIKRIVKAVKELLNEVKNETKLLIENTAGAGTEIGSNLDELKE
                     IMYEVDNFTRMGLCLDLCHLFAADYDISNQAGIEALIQEVNNKIGLKHLNLIHLNDSK
                     FELGTNKDRHAHIGQGKIGLENFETIINHEKLKDKLFIIETPAFDGVDKDIQTILNLK
                     KGE"
     gene            7659..10094
                     /locus_tag="VKN64_01665"
     CDS             7659..10094
                     /locus_tag="VKN64_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HMA58969.1"
                     /translation="MNIIDYFNNEIKNQIRDEIDNANGNEVFFLIEIDFDQEKIKNVE
                     VLARGNQSMAPAIINSLPKNSMVLHNHPSGDTTPSGADIRVASKVGDKGIGFAISDNS
                     VSELYFVVEPKDIGEVKEIDKEEILSYFKDDGPLANNMDGFEYRTEQLEIAANIVDNF
                     NQSEFTLIEAGTGIGKSFAYLIPALYWAVKNDETVIISTNTINLQQQIMDKDIEFLKK
                     VLPFKFKADLAIGRKNYLCIRKLKNIGNAMVEADHEEQQSYEKLVEWSKKTKSGMRNE
                     IKFNLKNSVWDEVASESDYCIGTLCPYYDDCYFMKARNELFNSDLIVVNHYLLLSDAV
                     IKNSGYSTLPKYKSLIVDEAHNFHDVATYHLGKSTSYKLNNQIFKKLYDKKYSIIPRV
                     RNKVAKLSMDNKEEVYQLIDQRIIPKIKDLEENNKEYFLLLDKFFQKYEHSSIIIAEE
                     LKEDDTYKDINQKGFNFYDNYDELLVYLKRLINQLKMVENYDKIEAELLELRQNLNTV
                     NDMAFNLLFNLEADDEDYVFWLEKYRYTDRYVSHMSALLNVEGQMPDMLWDKLRNVLM
                     TSATLTVNDSFDFFTEALGINNQQEYIVNSPFNYEKQSRLIIPNDIYSPSNNAFTSQI
                     ADDLTDILKTFNGSTMVLFTSYNMLDNLYKKVNHKLEEDNINVLSQSKYSRKYIMDKF
                     KAGKNQIIFGTQSFWEGVDIVGKDLKYLIIMRLPFPVPNDPVNAARVKMMKEEGKNYF
                     FEYFIPKAVIKFKQGFGRLIRSKNDKGIVICMDNRIINKRYGQVFLNSIPDRCPVIKM
                     KLRQIIDYVAGED"
     gene            10097..11020
                     /locus_tag="VKN64_01670"
     CDS             10097..11020
                     /locus_tag="VKN64_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HMA58970.1"
                     /translation="MKNQDKIIIITLLALLIFTSIGSAQPRFTLIYVVQNGDTIYDIA
                     KEYNTTVEHILEYNHIDNASKINIGDELVIKKEEEDNNDKNLNLNYSLSPHNDRTENF
                     KLDVGRNYSVRYNPEQPLPDVNIPPDKIIQYFVDSGDTLYDLAQSFNTSVGVIMALNN
                     MENSIIRQGQKLKLPILNLSKRQVLARTISQSDIEIMARTIHGEARGEPYMGQVAVGA
                     VIINRILSNEFPDTIKGVIYQKNQFTAVSDGQIHYTPNNTAYRAAREAINGVDPTLGS
                     LYYYNPETAQNKSFFNTRKFVVSIGDHVFAK"
     gene            11033..11467
                     /gene="dut"
                     /locus_tag="VKN64_01675"
     CDS             11033..11467
                     /gene="dut"
                     /locus_tag="VKN64_01675"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMA58971.1"
                     /translation="MKIKVKRMGEDVKLPEYQHYGEDCGMDLMSRYDYTLKPGEYKLI
                     KTGLKIAIPQGYGGFVYPRSGLALKEGITVLNADGVIDPGYRGELGVILINHGQKKFK
                     INKNDRIAQLIIHKTFNIEWDEVDELEDSNRSDGGFGHTGKN"
     gene            11480..12718
                     /locus_tag="VKN64_01680"
     CDS             11480..12718
                     /locus_tag="VKN64_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMA58972.1"
                     /translation="MESNVKDLSLAPAGKKKINWVKEKMTVLNKIKEEYKEDQPLKGK
                     TVSICLHLEAKTAYMAVTLSELGADVYITGSNPLSTQDDVAAALTEYGVTVYSWRGET
                     KEEYDESLNKILEVEPDLIIDDGGDLVSKIHNERPDLINKIIGGAEETTTGIIRLRSL
                     DNNNLLKFPMMSVNDAQAKYLFDNRYGTGQSVWDGIMRTTNMVVAGKDVVIAGYGWCG
                     KGVARRAKGLGARVIVTEVDPFKAIEAKMDGFRVMPMNKAAKTGDIFITVTGCKDVIN
                     KEHFKLMKNGAILANAGHFNVEINVEELKEMADEIKEARENIEGYKIGDKTLYLLAEG
                     RLVNLAAGDGHPVEIMDMSFGLQVLSLLYLSENDLRPGLYKVPDHIDKKVAKYRLKAE
                     GTKIDSLSPEQKQYLENWQI"
     gene            12728..13648
                     /gene="thrB"
                     /locus_tag="VKN64_01685"
     CDS             12728..13648
                     /gene="thrB"
                     /locus_tag="VKN64_01685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HMA58973.1"
                     /translation="MLKIKVPATSANLGPGYDSFGLALNLFNEYQFKIDKDIEKLSID
                     IINNNEVEVNLKKEDNLILNTIKYLDESLPHKINTRGLQIKAKLNYPLNRGLGSSANA
                     IIATIFGLNELFDLNLAKKDILSYALELEGHPDNIVPTLVGGFTISKVEDNEPIYEKF
                     EIAEEMQILLFIPELSIKTKEARRVITNTIKINHATQNIANASLLTAGLIKNDIELMK
                     KGSKDYIHQSKRIALNPKLEKIFIELKEKLETPLFLSGSGSTLIFFSKKDFKNEKEII
                     KNTADKYGIVNYLIETDVNNQGIIINKDGD"
     gene            13650..14702
                     /gene="thrC"
                     /locus_tag="VKN64_01690"
     CDS             13650..14702
                     /gene="thrC"
                     /locus_tag="VKN64_01690"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMA58974.1"
                     /translation="MWKGLIKKYKEFLPVSDQQEIVTLNEGNTPLIKADWFNKKYGFE
                     LYLKFEGANPTGSFKDRGMTLAVTKALEEKSKAIICASTGNTSASAAAFGARSGLKTL
                     VVVPKAKIAKGKLSQALIYGAQIIPIKGNFDKALEIVRELTDKHALTLVNSLNPFRIE
                     GQKTAAFEVADQLDRKKLDILALPVGNAGNITAYWKGFKEYNKKGIIDYLPKMYGFQA
                     ACSAAMVEERVIENPETIATAIRIGNPVNRDKARKAQEESEGLFESLTDDQIKDAHRL
                     LAAKEGVFVEPASAAPVAGLIKLSKEKKELVQGKKIVAILTGNGLKDQEFVLDNYSIP
                     KAVEGDLEKVEAIINL"
     gene            14778..16130
                     /locus_tag="VKN64_01695"
     CDS             14778..16130
                     /locus_tag="VKN64_01695"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HMA58975.1"
                     /translation="MKIFNFNFLDDWRAKIYYIPVKYSIYALIISAAVLLLEQNYQPY
                     LINILPNMFFTPLEITKTILSTIAGSLLSIVTISFSIIMVVLTTYSTQFSPRTLQDFL
                     KSKLTLQTLGIFIGGFIYSIVTLMFLSSDGQTVFLSAAVGVIIVIFCLIYFVMFINHV
                     SQSIQVNLLIDTLTTEILELVDNVKEFNEAHENIRNEPPANLDYILEKSQYSIYSKEV
                     GFIRDINDLKLSKFADEHNIVINAERMIGDYVTNNTKVFTIYYDIEEMEMEELNEETL
                     SKCLDFILVGKERNKSKDIEFGIQKLTEVALRAISPGINDPNTAIFCIKQAGMVLDKI
                     AKANIENTYYYNEDDELTLIFEDISFDYLLYKTFYQLRYYSMRDVSVAASILEALIII
                     AEGNEQKIKDITWEFGNYIVQGFDENILEDLDKKYMNSKIEELAVMTEKSKGAGIYFE
                     "
     gene            16140..>16381
                     /locus_tag="VKN64_01700"
     CDS             16140..>16381
                     /locus_tag="VKN64_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HMA58976.1"
                     /translation="MSKIDYKDKNFYQKTVTESFEILNTSEKGLEEKEAEKRLEEYGE
                     NKLVRLKKTPEIIKFLLQFRDVLMIILLVAAGMSFI"
BASE COUNT         5970 a         2492 c         3340 g         4579 t
ORIGIN      
        1 attatataat tgccggttct attacaggcc gagttaaggc cttactggat gaacacggtg
       61 aaagaatcga tgaggctgga cccgcgacac ctgttatagt gcttggtttt gacgacgtac
      121 cagaagcggg cgattatgta agagtcatgg aagatgagaa acgagcccgt gatttagccg
      181 aaagaagaca ggaaaaagaa aaagaagaaa gactcaaaaa agattctaag gtatccttag
      241 aagacttata tagaaatatt caggaaggtg aaattaaaga actgaatgta ataattaagg
      301 ctgatgtaca gggttcaatt gcagccttag aaaattcact aatgaatatt gaatctgatg
      361 aggtaagtgt caatatcatt cacaccgggg taggagcaat caatgaaaca gatgtaaatc
      421 tggccagtgc ttctaacgcc attattattg gtttcaatgt acgaccaggt actaatgccc
      481 gtaaagcagc ggaaaaagaa aaggtagaaa ttaaaaccta tcgagtaata tataaagtaa
      541 ttgaagatat taaaaatgcg atgtcaggac tacttgaacc tgaatataaa gagattgtgg
      601 tcggtcgtgt tgaagtaaga gataccttta aagtgcctga tgtgggaata gttgccggtg
      661 cttatgttaa agaaggtgaa gttaatagaa atgactatgt aaggttgata agaaatggta
      721 cgattattca tgaaggagaa attagttcct taaaaagatt tgaagatgat gtacgtgaag
      781 taaaagaagg atatgaatgc ggtattggaa tcgaaaatta caatgatata aaagttggag
      841 atattttaga aatatataga tataaggaga ttgagagatc cttatagagg tgagaaaaat
      901 ggctaacgaa aaaagagtaa gaagaatgaa tcaattatta aaagaaataa tcagtaagat
      961 aatattgagg gaattaaaag atccccgcat cggatttgct tcagtcacaa agatagatgt
     1021 ctcaaacgat ctaaaacatg ccaaggtgat ggtaagtgta ttaggtagtg aacaggaaaa
     1081 gaaagaaaca atggaaggat tagatcaggc cagaaactat atacaaaaac tggtaggtga
     1141 aagacttact acttaccata cccctaagat aatatttaga tatgataatt ctattgaaca
     1201 gggagttcac atctcaaaac ttattgatga ggtaattgaa gaagatgaaa agaggcatga
     1261 agatggtgaa gaagaataat tctttacagg aaatagttga atatataaaa gaaaatgatg
     1321 attatttaat aataggtcat atagaccctg atggtgatgc tatcgggtct attatggcct
     1381 ttaaatttct attaaaccat ttaaacaaga attccctatt acttttacac agtgacttaa
     1441 ctaaagaata tgatatatta tttaaatacc ttaattccga tgagtactat atatttaata
     1501 agggaaatga agaacagtta aaagaatata ataaggttat tgcccttgac tctgctaata
     1561 tagagcggtt gggagactat aaacctatta ttgaaaacaa gcagatagta aatattgatc
     1621 atcattatga taacagccgt tttggggact taaattatgt gaatccagat gaatcagcag
     1681 tcggtaaaat aatctatgat atggttgatc tcttagatat tgagatcacc gaagacctag
     1741 gaaccgctat ggctttagca gtattggctg ataccggttc tttaaaatac cccaatagtg
     1801 acccggccag ttttagaatg atggccagtt taaaagaaga ggggattgat attgttaaaa
     1861 tcaatagatt tttacagagc ttttcttctt taaagtattt aaaactgcta tccgaggctc
     1921 ttgccaatat taaaacggcc aaacaaggta aaatagcctg gctggtggtc aataatcata
     1981 caataaaaag tatcggtatt gatgaaaaag aaattaataa tctagttaac tacccgcgcg
     2041 atatacgcgg ggtggaagta ggtatttctt ttgtcgaaac gaaaaaagac tttattgata
     2101 tcagttttag atctttaaat tatgttgatg taagtaagat agcccataaa tttaacgggg
     2161 gaggccatcc ccgggcagcc ggtaccaaga ttaaaggcgc tttagaacaa acagttcggg
     2221 cagttctgga tgaggtgaag aaaaatgtct aaccaacctg gtataataaa tgtattaaag
     2281 ccaccaggta tgacatcatt tgatgtagta ggatatataa aaaagatatt gcagatcaaa
     2341 aaggccggtc ataccggtac tttagatccg gccgctgccg gtgtgctacc ggtctgtctg
     2401 caccgcggta ccaagattat cccctatctg ccggaagaaa aaaaggtata tatcgccagg
     2461 attagtcttg gtctgaaaac tgacacctta gatgctgatg gtgaaatatt agaaaaagat
     2521 aataactggc agagtttaga cgacaatcaa attaaagaag ctgtccagtc tttttcgggc
     2581 gagattatgc aaaaaccacc tatttattct gctgtgaaag taaatgggaa aagactttac
     2641 cattatgctc gtaatgatca agaagccaag gtggaaattg aagatagagt ggtaaatatc
     2701 tatggtatcg aagtattaaa tataaattta ccccttatta aaatcaaagt aagctgttcg
     2761 aaaggcacat atatccgttc tctggccaga gacattggta gtaagttaaa tacttttgct
     2821 catttaaaaa atttgctgag gacggcctcg gggcccttca aattagaaga tacggttact
     2881 ctctatgaaa taactaatga aaatctggat aatatactta tatccctgga cagtcctttt
     2941 gattacagac cgatggaagt taaggattat gcctataaat ttgctataaa tggtacgaaa
     3001 ttgttaatta agaactttat aaaatggcct tcagatatta caatagggga aaggctattg
     3061 atatatggca aaggtcaatt catatccata agtgaagtta aagaagatga agacgaaaaa
     3121 atatatatcc aacctttgag agtatttcat gatggaggtc agaactaaaa atgaatatta
     3181 ttagaagcaa tacatttgat caatatgaaa tagatgagaa catcatagct atcggttctt
     3241 ttgatggtat tcataaaggt cataaaaaaa tattaaataa gactttaact ttagctgaaa
     3301 agaacaatct aaaaagcggg gttttaacct ttgaacctca tccccaggaa gttgtggccg
     3361 atgaagataa tcattactac attacttccc tggcccaaaa aagaaaaatt ttaaatgaaa
     3421 tgggttttga ttataacttt gaaatgaact ttacaaaaga tattgcccag accccattcg
     3481 atgattttgt taaaaagatt attgtagata atctaaatac caaacacatc gtggttggaa
     3541 ctgattttac catgggttat aaaggtgacg gtaatataga tgagttaatt aaattaggaa
     3601 aaaaatatgg ttttgatgtt acagcagttg aaccattgaa aataggtgat cataaaattt
     3661 ccagtagtgt gatcagagat ctgatcatgg gtggacgcct aaaggaacta aaagaacatc
     3721 ttgaccgcaa ctttatttta gaaggggaag tagttaaagg atatggtcga ggtaaaaaat
     3781 taggtattcc aaccgctaat attaaacctg ccactaagta tatactaccc ccggatggag
     3841 tttatgcagt ttatgtttat ctaaatgaag ataaatataa aggagttgct aatttcggac
     3901 ataaacccac ctttgatgat gatgacttta ccattgaggt tcatttaccg gactttcaag
     3961 gagatatcta tggtgaattt gtcgaaatag agttgattga atatttaaga ggagagcaag
     4021 aatttgatga tgtagacgaa ctaatagaga caataaagca ggatattctt tacatcaaag
     4081 aggttctatg ataaaattat tctggtaaaa ttaaatacct tatactaggt taaagattcc
     4141 tcaactttta cttggtgtag ggcgtatcaa caactaggag gtgaagtaaa ttgatttcca
     4201 aagaaagaaa acaagagatt gtgcaaaagt atcaaagaga tgagaaagat actggttctc
     4261 ccgaagtaca gattgctatt ttaaccgaaa gaatttctca gcttacagac catttaaaag
     4321 atcataaaca agatcattct tcccgtagag gtttacttaa aatggttgga cagcgcagaa
     4381 agcttttaag atatttagaa aataaagatg tcgaaagata tcgggaatta atctctagtt
     4441 taggtattag aggttaattt taatatgaaa ttgtaaagag cggggttcta cccgctcttt
     4501 attataatga ataattaatt taaaggaaaa aataaggagg aaaaaaacga atgaccaata
     4561 cttggaaaac agattttgcc ggaacagaac ttgaatttga aaccggtaaa gtggccaaac
     4621 aggctaatgg tgcagttact gttaaatatg gagattcaat ggttttagtt actgcaacca
     4681 tgtcagaacc tagagaaggt ataaattatc tacctttgat ggttaattat gaagaacgcg
     4741 tttattctat cggtaaaatt cccggtagtg tgaacagaag agaaggacgc ccacgtgatt
     4801 ttgctaccct aacagcccga ttaattgata gaccattacg tcccctgttc ccggatggta
     4861 tgagacacga tattcaggtg attacaaccg tactttctgt tgacaaagac catgaaccag
     4921 atatcatagc gatgaatgga gcctcagcag ctctcacctt atctgatata ccttttgatg
     4981 gtcctattgg aggagttaaa gtcggtttag ttgatggtga acttgtttta aatccaaccg
     5041 aagaagagcg gaaaaattca aagttggatc taacagttgc tggaacagaa gaggctgttt
     5101 caatggtaga agccggtgct gatgaagttg aagaagaagt aatccttgat gcaattgact
     5161 tagcacatga agaaatcaaa aaaatcgtcc gtttccagaa aaaaatgcaa aaagaagcgg
     5221 gtttagaaaa acaggatatt tccttagtaa ataatgtaga tgaaaagata gaaaaattaa
     5281 tcagcgattt tgctacaaat agattaagag aagctatcaa taccgttaag aaacaggaac
     5341 gcagtgaaca aattgatgaa ctaaaagaag acgcaaaaga atatttggca ggcaaattag
     5401 aagattatga agaaaaagaa gaagaatata ataaaatggt tgaggctgcc tttgatgacc
     5461 tcactaaaaa gctagtcaga aaaatgatta ttgaagatgg taaaagacca gacggtagat
     5521 cttatgatga tatcagacct atctggtgtg aagttggcaa cttaccaagg gcccacggtt
     5581 cagctatctt tacaagaggt gaaacccagg ccttaagtgt agttacctta ggagccagca
     5641 gtgatgaaaa gacaatcttt ggtcttggtg aagatgagac taaaagatat atgcatcatt
     5701 ataatttccc accctatagt gtaggagaaa ctaatccctt aagatcaccc ggccggagag
     5761 aaatcggaca cggtcattta ggggaaaggg ctctcaaacc gatgatccct tctaaagaag
     5821 agttccctta cacaattcgg gttgtctcag aagtacttga atcaaatggt tcttcatctc
     5881 aggccagtat ctgcggctca accctatctc ttatggatgc cggtgtcaag attaaacaac
     5941 ctgtttcagg aatcgcaatg ggtttaatca aagaaggcga tgactatgca gtcctttctg
     6001 atatccaggg gatggaagac cattatggag atatggactt caaagtagcg ggaactccca
     6061 atgggataac cgccctacag atggatatca aaataggggg actttctaaa gatatcctaa
     6121 ggaatgcttt aaacaaggca agaaaaggaa gactctatat actagataag atgctggaag
     6181 taattgatga accaagagaa aatctatccg agcatgctcc acttatcatg accatgcaga
     6241 ttgatcctga aaagatcaga cacgtaattg gaccaggtgg aaagatgatt aataagataa
     6301 tcgatgaaac cgatgcccgg atcgatatcg aagatgatgg taccatcttt attctagcgg
     6361 aaggccagga aaacggtcaa aaagcgaaga agatgattga tgatctaacc agagaagttg
     6421 aagtcggcga agtttatgaa ggtaaagttg ttaaaattat gaacttcggt gcctttgttg
     6481 agatcttacc tggtaaagaa ggccttgttc acatctctaa tattgctgac aggcatatca
     6541 atgaagtatc cgatgtctta agtgaaggag acgaaatcag agttaaggta atcaagattg
     6601 ataataaaga ccgtattaac ctttccatga aagatgttga acaggacttt gaagtataaa
     6661 taatttaata aataaagata actatctttt aagatagttt aagaaactta cctcaagtaa
     6721 actcctttaa atttcttaat ttaaaggaga tgtattgagg ttgttttttt acatatagtt
     6781 acattttttg ccaatcaaga aggagatgtt caaaaaaatg aaattaggga gacacctttc
     6841 cattagcggt ggtattgata aagcaattga tgaaaccatt gcaattaatg caaactctgt
     6901 tcagatcttt accaaatcac ccaggagttg gcagaaaaaa gaaataagtg aagaagagat
     6961 aaaaaagacg agagaaaaaa taaaaaagta tgaaataaaa gcttttgtgg tccattccag
     7021 ctacctggtt aacttagcaa cccccaatga tgatctatac tataaatcca aagaagaaat
     7081 aaaagaagat taccgccagg ctggccggct gggagccgat tattatgtct ttcaccccgg
     7141 taattatgtt aagtcttcca aagaagcagg cataaagaga atagtaaagg ctgttaaaga
     7201 attgctgaac gaagttaaga atgagactaa acttctcata gaaaatacag ccggggccgg
     7261 gactgagatc ggcagtaatt tagatgaact aaaagaaata atgtatgagg tcgataattt
     7321 tacaagaatg ggactttgcc ttgacctctg ccatttattt gcagccgatt atgatatttc
     7381 taatcaagca ggtatagagg ctttgattca agaagtaaat aataagatcg gcctaaaaca
     7441 tcttaatttg attcatttaa atgattctaa atttgagtta ggcacaaata aagatagaca
     7501 tgcccatatc ggccagggta aaattggctt agaaaacttc gagacaatta ttaaccacga
     7561 gaaattaaaa gataaattat ttataataga aacccccgct tttgatggtg ttgataaaga
     7621 cattcagact atacttaact taaaaaaagg tgaatgagtt gaatataata gattatttta
     7681 ataatgagat taaaaatcaa ataagagatg agatcgataa tgcaaatgga aatgaagtat
     7741 tttttctgat tgagatagac tttgatcagg aaaaaattaa aaatgtggaa gttttagccc
     7801 gggggaatca gagcatggcc cccgctatta ttaattcact accgaaaaat tctatggtcc
     7861 tgcataacca cccgtctggt gatacaacac cttctggagc agatatcagg gttgcatcta
     7921 aagtgggcga taaagggatc ggttttgcaa tttcagataa cagtgtaagt gagctctatt
     7981 ttgtggtaga accgaaggat atcggggaag tcaaagagat agataaagag gagatccttt
     8041 catattttaa agatgatggt cccctggcca acaatatgga cggatttgaa taccggaccg
     8101 aacaactgga aattgcagcc aatattgttg ataactttaa ccaatctgag ttcacattaa
     8161 tagaagcagg taccggaatt ggtaaatcct tcgcctattt aataccggcc ctctactggg
     8221 ctgtaaaaaa tgatgaaacg gtaattattt ccaccaatac gatcaactta cagcagcaga
     8281 ttatggataa agacatagaa tttttaaaga aagtactgcc ctttaagttc aaggctgacc
     8341 tggctattgg acgtaaaaat tatttatgta ttagaaaact caaaaatatt ggaaatgcaa
     8401 tggtagaagc tgaccatgag gaacaacaga gttatgaaaa gttggttgaa tggtccaaaa
     8461 agactaaatc tggcatgcgg aatgaaataa aattcaactt aaaaaattcc gtctgggatg
     8521 aggtggcttc ggaaagtgat tactgtatag gaactttatg cccctattat gacgactgtt
     8581 attttatgaa agccagaaat gaactattta attctgatct tatcgtagtt aaccactacc
     8641 tcttattatc cgacgctgta atcaaaaatt ctggttattc caccttgcct aaatataaga
     8701 gtttaatcgt tgatgaggcc cataactttc atgatgtagc gacttatcac ctggggaaaa
     8761 gcacatccta taaattaaac aaccagattt ttaaaaaatt atatgataaa aaatactcga
     8821 ttattccccg ggtaagaaat aaggttgcca agctgtctat ggataacaag gaagaagttt
     8881 atcagcttat tgatcaaagg ataattccga agataaaaga tttagaggaa aataacaaag
     8941 aatatttcct gcttttagat aaattttttc aaaagtatga acacagcagt ataattattg
     9001 cagaagaatt aaaagaagat gatacataca aagatatcaa tcaaaagggc tttaattttt
     9061 acgataatta cgatgaatta cttgtttatt taaaaagatt aataaatcag ttaaaaatgg
     9121 tcgaaaatta tgataaaata gaggctgaat tacttgaatt aagacagaac ctaaatacgg
     9181 ttaatgatat ggcctttaat cttcttttta atttagaagc agacgatgaa gactatgtat
     9241 tctggttgga aaagtacaga tatactgacc gttatgtaag ccatatgagt gctttactga
     9301 acgtggaagg tcagatgccc gatatgttat gggataagtt aagaaatgtt ttaatgacct
     9361 ctgcgacctt aacggtcaat gattcctttg atttttttac agaagcctta gggatcaata
     9421 atcaacagga gtatatagtt aattctcctt ttaactatga aaaacagagt cgactcatta
     9481 tcccaaatga tatttattca ccttctaata atgcctttac aagtcagatt gccgatgatt
     9541 taactgatat attaaaaact tttaatggtt cgactatggt tttatttact tcctacaata
     9601 tgcttgataa cctatacaaa aaggtaaacc ataaattaga ggaggataat ataaatgtgc
     9661 tttcccagag taaatactcc agaaagtata ttatggataa atttaaagcc ggtaaaaacc
     9721 agataatctt tggtacccag agtttctggg aaggtgtcga tattgttggt aaagatctta
     9781 aatacttaat tattatgcgt ctgccttttc cggtccccaa tgatcccgtt aatgcggccc
     9841 gggttaagat gatgaaagag gaaggcaaga attacttttt tgaatatttt attccgaaag
     9901 cggtcatcaa gttcaaacag ggctttggcc gattgatccg ttccaaaaat gataaaggaa
     9961 ttgtaatctg tatggataat agaataatta ataagaggta cggccaggta tttttaaatt
    10021 caatacctga taggtgtccg gtaattaaga tgaaattaag acagattatt gactatgtag
    10081 ctggagagga ttaaatatga aaaatcagga caagataatt attataacat tactagccct
    10141 ccttatattt acctcaatag gatcggccca accccgtttt accttgattt atgtggtgca
    10201 aaacggtgat acaatctatg atatagccaa agaatacaat acaaccgttg aacatatatt
    10261 agaatataac catattgata atgcttccaa gatcaatatc ggggatgagc tggtaataaa
    10321 aaaagaagaa gaagacaata atgataaaaa tttaaattta aattacagtt tatcacccca
    10381 taacgaccgg actgaaaatt ttaaattaga tgtaggacga aattattccg taagatataa
    10441 tccagaacaa ccgttacccg atgtaaatat accaccggat aagattatcc agtactttgt
    10501 cgattccggg gacacacttt atgatttagc ccagagcttt aatacctctg tcggggttat
    10561 catggcctta aataatatgg agaactccat tattagacaa ggacaaaagt taaaactgcc
    10621 gatcctaaac ctttcaaaaa ggcaggtctt ggccagaacg atcagtcaat ctgatattga
    10681 aatcatggcc aggacgatcc acggggaagc tcgcggtgaa ccttatatgg gtcaggtagc
    10741 tgtcggggcc gtaattatca acagaatttt aagtaatgag tttcccgata cgattaaggg
    10801 tgttatttat caaaaaaatc aatttacagc cgtttcggac ggccagatcc actacacacc
    10861 caataatacg gcctatagag cagctagaga ggctataaat ggggtagacc ctacgttggg
    10921 ctctctctat tactacaatc ctgagacagc ccaaaataag agtttcttta atacaagaaa
    10981 atttgtagtt agtataggag atcatgtatt tgcaaaatag gaggcaaacg atatgaaaat
    11041 aaaggtaaaa agaatgggtg aggatgtaaa gttaccagag tatcaacatt acggtgaaga
    11101 ctgtggaatg gatcttatgt cacgctatga ttatacgctt aaaccggggg aatataaatt
    11161 aataaaaacc ggtttaaaaa ttgcgatacc acaaggttat ggtggatttg tctatccccg
    11221 cagcggtctc gccttaaaag aaggcattac ggtcttaaat gccgatgggg taattgatcc
    11281 cggttaccgt ggtgagttgg gagtcatctt aatcaaccac ggtcaaaaaa aattcaagat
    11341 caataaaaat gatcgaattg cacagttaat tattcataaa acctttaata tagagtggga
    11401 tgaagtagat gaattagaag atagtaaccg cagtgacggc ggttttggac atacaggcaa
    11461 aaattaaggg ggataaaata tggaaagtaa tgtaaaagat ctttcattag ctccagccgg
    11521 taaaaagaag atcaattggg ttaaagaaaa aatgacagtt ctcaataaaa ttaaagaaga
    11581 atataaagaa gatcagcctc tcaaaggcaa aacggtctca atctgtttac atcttgaggc
    11641 aaaaacagcc tatatggcag ttactttaag tgaactaggg gccgatgtgt acataacagg
    11701 cagcaaccct ctctctaccc aggatgatgt agctgccgcc ctaactgaat atggtgtaac
    11761 ggtttacagc tggcggggtg aaacaaaaga agagtatgat gagtctttaa ataagatatt
    11821 agaggtggaa cccgatttaa taattgacga tgggggagac ctcgtaagta agatacataa
    11881 tgagagaccg gatctgatca acaagataat cgggggagca gaagaaacca caactggtat
    11941 tatcaggtta agatcgcttg ataataataa tctattgaag ttccccatga tgtcagttaa
    12001 tgacgcacag gccaagtacc tttttgataa caggtatgga accggccagt cggtctggga
    12061 tggtatcatg cggaccacta atatggtagt ggccggcaaa gatgttgtaa ttgccggtta
    12121 cggctggtgt ggaaaaggtg tggccagaag agctaaaggc ttaggggcaa gggttattgt
    12181 aacagaagtt gatcccttta aagcaattga ggccaaaatg gatggttttc gggtcatgcc
    12241 gatgaataag gccgctaaaa ccggtgatat tttcataacg gtaaccgggt gtaaggatgt
    12301 aataaataaa gaacatttta aattaatgaa aaatggtgcc attttagcca acgctggtca
    12361 ttttaatgta gaaattaatg tagaagaatt aaaagaaatg gccgatgaaa taaaagaagc
    12421 gagagaaaat atagaaggat ataagatcgg tgataaaacc ctttatctct tggcagaagg
    12481 caggcttgtc aatctggcgg caggggatgg acaccccgtg gagataatgg atatgagctt
    12541 tggtttacag gttttatcat tactctacct atccgaaaat gacctaagac ccggattata
    12601 taaggtgccg gatcatattg acaagaaagt ggcaaaatat agattaaaag cggaagggac
    12661 aaaaatagat tcactgagtc cagaacaaaa acagtattta gaaaactggc aaatttaggt
    12721 gagaaaaatg ttaaaaatta aagtaccggc aacttctgcc aatttagggc ccggctatga
    12781 ttcatttggt ctggctctaa atctatttaa tgaatatcaa tttaaaatcg ataaagatat
    12841 agaaaaattg tcaatagata taatcaataa caatgaagtt gaggtaaatc tgaaaaaaga
    12901 agataattta attttaaata ctatcaagta tctagatgag agtttacccc ataagataaa
    12961 tacacggggc ctacagatca aagcaaagct taactatccc ttaaataggg ggttaggtag
    13021 ttctgctaat gccataattg caactatttt tggtttaaat gaactgtttg accttaactt
    13081 agctaaaaaa gatatactgt cttatgccct tgaattagag ggacacccgg ataatatagt
    13141 tcccacctta gtgggaggat ttacgatcag caaggtagag gataatgaac ctatctatga
    13201 aaaatttgaa atagctgaag agatgcagat actcctgttc attcccgaac tgtccattaa
    13261 aacaaaggaa gcccggcggg taatcacgaa tacaattaaa attaaccatg caacccaaaa
    13321 tattgccaat gcctctctct taacagccgg attaattaaa aatgatattg agctgatgaa
    13381 aaaaggatca aaagattata tccaccagtc caaacgaatc gctcttaatc ccaaattaga
    13441 aaaaatattt attgaactca aggaaaaatt agaaaccccg ctatttttaa gcgggagcgg
    13501 ctcaacctta atcttttttt ctaaaaagga ttttaagaac gaaaaggaaa taattaaaaa
    13561 tacagccgat aaatatggta ttgttaacta cttaattgaa actgatgtta ataatcaagg
    13621 tataataata aataaagatg gtgattaaaa tgtggaaagg attaataaaa aagtataaag
    13681 aatttttacc agtttctgat caacaagaga tagttacctt aaatgaaggt aatactcctt
    13741 tgataaaagc tgactggttt aataaaaagt atggttttga actttactta aagttcgaag
    13801 gggccaaccc gaccggttct tttaaagatc ggggtatgac cctggctgta acaaaggcat
    13861 tagaagaaaa atctaaagcg attatctgtg cttcaaccgg taatacttcc gcttcagcgg
    13921 ctgctttcgg ggcccgcagt ggattaaaaa cattagtagt ggtaccgaaa gccaagatcg
    13981 ctaaagggaa gttatcgcaa gctttaatat acggggccca aattatacca atcaaaggta
    14041 attttgataa ggccttagaa atagttagag aattaaccga taaacatgcc ttaacactgg
    14101 ttaattcctt gaatcctttc agaattgaag gtcaaaaaac agctgctttt gaagtggctg
    14161 accagcttga ccgaaagaaa cttgatattc tcgctttacc ggtcggcaat gccggcaata
    14221 ttactgccta ctggaagggg tttaaggaat ataataaaaa agggataata gattatctac
    14281 caaagatgta tggttttcag gcagcttgct cagccgcaat ggttgaagaa agggtaattg
    14341 aaaatcccga aacgattgcg actgccatca ggattggtaa tcctgttaat agagacaaag
    14401 caagaaaagc acaagaagaa tcggaaggat tatttgaatc tttaacagat gatcagataa
    14461 aagatgccca ccgactactt gcagccaaag aaggggtctt tgtggaaccg gcctcagctg
    14521 ctcctgtagc aggactaatt aaattaagca aagagaaaaa agagttagtt caaggtaaaa
    14581 agattgtagc tattctgacc ggtaatggcc tgaaagacca ggagtttgtc ttagataatt
    14641 attctattcc taaagcagtt gaaggtgatt tagaaaaggt agaagcgatt ataaatttat
    14701 agataaagag gaaaaacgga actgttcaag aaatataaac taataaagca gaaaaccctt
    14761 tgggaggggg tatttaattg aagatattta actttaattt cctcgatgat tggagggcca
    14821 agatatatta tattcctgta aaatatagta tatatgccct aataatttca gcggctgttt
    14881 tgttattaga acaaaattat caaccttatt taataaatat cttacctaat atgtttttta
    14941 ctcccctgga aattactaaa acgatactat caacaatcgc tggttctcta ttatctattg
    15001 ttaccatatc tttttccatt atcatggttg tattaactac atattctact caattttcac
    15061 ctaggacctt acaggatttt ctaaaatcaa aactaacatt gcagacctta gggatcttta
    15121 ttgggggatt tatatattca attgtcaccc tgatgttttt aagttctgat ggccagactg
    15181 tatttttatc agcagcagtc ggtgtaatta tagtaatatt ttgtcttatt tactttgtga
    15241 tgtttatcaa ccatgtttca caatcaattc aggttaatct attgatcgat accttaacta
    15301 cagagatctt agaactggta gacaatgtca aagagtttaa tgaagcccat gaaaatatta
    15361 gaaatgaacc acctgctaat ctggattata ttttagaaaa aagccaatat agtatttatt
    15421 caaaagaagt gggctttatc agggatatta atgatttaaa gctgtctaaa ttcgccgatg
    15481 aacataatat agtaatcaat gctgaacgga tgattggtga ttatgtaact aataatacga
    15541 aggtatttac catctattat gatatagaag aaatggagat ggaagaatta aatgaagaga
    15601 ccctatctaa atgccttgac tttatcctgg tgggtaagga aagaaacaag agtaaggata
    15661 ttgagtttgg aatccagaaa ttaaccgaag tagccttaag ggctatctct cccggtatca
    15721 atgaccccaa tacagccata ttctgtatta aacaggccgg gatggtttta gataagattg
    15781 ccaaagctaa tattgaaaac acctattact ataatgaaga tgatgaactt accttaatat
    15841 ttgaagatat cagtttcgac tatctcctat ataaaacatt ttaccagtta aggtactatt
    15901 caatgcgtga tgtttcagta gctgcttcaa tattagaggc tttaataatc atagcagaag
    15961 gtaatgaaca aaaaataaaa gatatcacct gggaatttgg caactatata gtacagggtt
    16021 ttgatgaaaa tatattagaa gatcttgata aaaaatatat gaacagcaaa atagaagagc
    16081 tggctgtgat gacagaaaaa tctaaaggag ccggaatcta ctttgaataa aaatgggagg
    16141 tgtctaaaat agactacaaa gacaaaaact tttatcaaaa aacagttaca gaaagctttg
    16201 agatccttaa tacttctgag aagggactgg aagaaaaaga agctgaaaaa aggttagaag
    16261 aatatggtga aaataagctt gtccgcttaa agaaaacacc tgaaatcata aaattcctat
    16321 tacagtttag agatgtacta atgatcatcc tcttagtggc agctggtatg tcctttatta
    16381 t
//
LOCUS       DATGCJ010000037         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_227194, whole genome shotgun sequence.
ACCESSION   DATGCJ010000037 DATGCJ010000000
VERSION     DATGCJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227194"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VKN64_01705"
     CDS             <1..1360
                     /locus_tag="VKN64_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58977.1"
                     /translation="FDEIKGETSKDKIKQLKSFNLEYEKHNTITNHSIVDVYDHIIKD
                     WYDLNTIRYNSYDGDTQYAEYGTPFEGVYLLKSNLDKNSAGYKKLASKTANNGKGWEP
                     LIGDPYSGRFYFETFIGVFEGNNHIIKDLYINRSDEDYASLFGMLGNKFVESDAKIMN
                     LGMENIGINGGNSVGGITGNNKGVIKNSYVKGLIESSSSAGGIAGGNYGVIVNSYFRG
                     NIEGTNKVGGIAGGNYGVIVNSNVEGKIKGIEKVGGLVGDNKNEIKNSYFKGELLGED
                     EIGGIAGINDYGSIIKNSFADLNIDAINSKDMGGIAGTNYEIIRNSYASGEINGDVSL
                     GGLAANNHGIIDRSYSTIKISGSDNIGLLVNSNFGTVKDSFVLEDDILLIDNSNTTTS
                     EILGRTTKVNEEEMRGNEIYTVNPIDGYENLIDLWSEDIWDLGDDSVNSHSYPSLYWQ
                     KE"
     regulatory      1364..1447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1806..>2798
                     /locus_tag="VKN64_01710"
     CDS             1806..>2798
                     /locus_tag="VKN64_01710"
                     /inference="COORDINATES: protein motif:HMM:NF023351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HMA58978.1"
                     /translation="MLAVLFSGSIEAQQVDRAPRININGITGDNFISQTGVLYPFKNT
                     ENSLWFTDLRYRISSDEVQEWNLGLGYRFKKDQGKENHIAGVYLYKDNRKEFDHNWDM
                     WTLGGEILSEEFDFRINAYITDDEKVLAPNSTAGGEPSFRINESNELLISTGNAVYYK
                     SMDGVDIEFGKRFTETDTIFKDVGVYAKFFSFFESSVGPMQGKQIRINKQFGNRDKIN
                     WKIGAKWREDNIRGSETKATFAVSIPFGKGSTGKTDNEEKDLLESRMTDQPERDIDVV
                     VGKAESEDATSDTSSDEFAAINPVEGEDKVQVWYVTADGNGDGSKEDPIGLDKRA"
BASE COUNT         1058 a          283 c          593 g          864 t
ORIGIN      
        1 ttttgatgaa ataaaaggcg agacttcaaa agataaaatt aagcagttaa aatcatttaa
       61 tttagaatat gaaaagcata atacgattac gaatcacagt attgtagatg tatatgacca
      121 tataataaaa gattggtatg atttaaatac aattaggtat aatagttatg atggtgatac
      181 ccaatatgcc gaatatggta ctccatttga aggtgtatat ttattgaagt ctaatttaga
      241 taaaaattct gcaggctata aaaaattagc cagtaaaaca gctaataatg ggaaaggctg
      301 ggaacccttg ataggtgatc catatagtgg tagattttac tttgagacat ttattggtgt
      361 ttttgagggg aacaatcata ttataaaaga tctttatatt aatagatctg atgaagatta
      421 cgcaagtcta tttggcatgt taggaaataa atttgtagaa agcgatgcta aaattatgaa
      481 tttaggtatg gaaaatatag gaataaatgg gggaaattca gttggtggta taacaggtaa
      541 caataaggga gttataaaaa acagttatgt taaaggtctt atagaaagtt caagttcagc
      601 aggtggaata gcaggtggta attatggtgt tatagtaaac agctatttta gaggaaatat
      661 agagggaaca aataaagttg gtggaatagc aggtggtaat tatggtgtaa tagtaaacag
      721 caatgttgag ggtaagataa aaggaattga aaaagtaggt ggtttagtag gtgataataa
      781 aaatgaaata aaaaacagtt attttaaagg agaacttttg ggcgaagatg aaataggtgg
      841 gattgcagga ataaatgatt atggaagtat aataaaaaac agttttgcag atttgaatat
      901 agatgcaata aatagtaaag atatgggtgg tatagcgggt actaattatg aaattatccg
      961 gaatagttat gcctctggtg aaataaatgg agatgtgtct ttaggaggtt tagctgcaaa
     1021 taatcacggt attatagata gaagttattc gactataaag atatcggggt ctgataatat
     1081 aggcttacta gttaattcta attttggtac tgtaaaagat tcttttgtat tagaagatga
     1141 tattttactt attgataaca gcaacaccac gacatctgaa atattaggaa gaaccacaaa
     1201 agttaatgaa gaggaaatga ggggaaacga gatttatact gttaacccta tagatggata
     1261 tgaaaatctt attgatttat ggtctgaaga tatttgggac ttaggagatg atagtgttaa
     1321 tagccattct tatccctctt tatattggca aaaagaataa ataattatat aaaggcaagc
     1381 taactgtgag gttagcacgc aaagttttga gtctaagatt tttaaccaga ctgtcagaac
     1441 tacctcaggc attaactata aaagtgtaaa aagctttggc agttttttta aataaaacat
     1501 taaagtatct aagtaaatat atattcagtt ttgaaatctg atatacttca gaacttttat
     1561 gtatcttaga gtctagatat gaaaagaagg gagggtagga tatatttaaa gttattttat
     1621 taaattttca aaattgaatt tatgataaaa tactacttaa aattatagac caacttacta
     1681 tcttttagtt ttaattaaaa ctaggtaatt tctatatacg aacttttatt tttaaaatat
     1741 ctgatataaa gaggagagat ataaaaatgt tcaaaaaact caagattagc ttttaattag
     1801 tattaatgtt agcagttttg ttttcaggca gtattgaagc acaacaagta gatagggcac
     1861 cgagaataaa tattaatgga attacaggcg acaattttat aagccagacc ggtgttttgt
     1921 atcctttcaa aaatactgaa aattcacttt ggtttactga tttaagatac agaataagtt
     1981 cagatgaggt gcaggaatgg aatcttggtt tgggttatag gtttaaaaaa gaccaaggta
     2041 aagaaaacca tatagctgga gtctatttat ataaagataa caggaaagaa tttgatcata
     2101 actgggatat gtggacttta gggggagaaa tattatctga agaatttgat tttagaataa
     2161 atgcctatat aactgacgat gagaaagtat tagcccctaa ttccaccgct ggaggggaac
     2221 cttcttttag aatcaatgaa agtaatgaat tgctaatctc gaccggtaat gcagtatatt
     2281 ataagtcgat ggatggtgtg gatatagaat ttggtaaaag atttactgaa acagatacaa
     2341 tctttaaaga tgttggtgta tatgctaagt tctttagttt ctttgaaagt agtgtaggtc
     2401 caatgcaggg aaaacagatt aggattaata agcaatttgg taatagagat aaaataaact
     2461 ggaaaattgg tgctaaatgg cgtgaggata atataagagg tagtgaaaca aaagccacct
     2521 ttgcagtttc aataccattt ggtaaaggtt caactggtaa gacagataac gaagaaaaag
     2581 acttactaga atctagaatg acagaccagc cggaaagaga tattgatgtt gtggtaggaa
     2641 aagccgaaag cgaagatgct acaagtgata cttcaagtga tgaatttgct gctattaacc
     2701 ctgttgaagg tgaagataaa gttcaagttt ggtatgttac tgccgatgga aatggagatg
     2761 gatcaaagga agatcctatt ggtttagata aacgcgcg
//
LOCUS       DATGCJ010000038         7937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_49142, whole genome shotgun sequence.
ACCESSION   DATGCJ010000038 DATGCJ010000000
VERSION     DATGCJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49142"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VKN64_01715"
     CDS             complement(<1..483)
                     /locus_tag="VKN64_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HMA58979.1"
                     /translation="MGDLIKYSKKEEIANAITHGLGIAFGIFALVFLIVTHYKTTDIY
                     GLVSYIVYGASLIILYLSSTLYHSIPIKSAKIHLRKLDHASIFILIAGTYTPISMVAL
                     RGKLGWTILITIWSIALLGIIFKVFFINKLKIISVIMYLAMGWLIIFAIKPLIVALST
                     K"
     gene            complement(586..1194)
                     /locus_tag="VKN64_01720"
     CDS             complement(586..1194)
                     /locus_tag="VKN64_01720"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HMA58980.1"
                     /translation="MNRCDWAEGSEMYIKYHDKEWGVPVFDDKILFEFLILETAQAGL
                     SWSTILKRRDNYKEAYDNYDIEKIASYEEAKRTELLNNKGIIRNKLKIDASINNAERV
                     LKIQEEYGSFSRYLWSFTVFTPVINKSETLSEVPAETELSKTIAKNLKKKGFKFVGSV
                     TIYAFLQAVGIVNDHLVDCFRYQEVIDYYNKKVKDVDYLKEI"
     gene            complement(1306..2448)
                     /locus_tag="VKN64_01725"
     CDS             complement(1306..2448)
                     /locus_tag="VKN64_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58981.1"
                     /translation="MQRKKIIALILIGLVAIMVIDFRINDEDVIADYFMQFETESIKN
                     ISNMPNVALNDERRSPEAVRKSSFSYNAADLENFILDNPVGSVDIQGHDKDTIEVTYE
                     VKVYSSSKEAAEEFLEKIGILYGEPNGTFELEVQAPRPKPANIRAFEVDFDILAPKDL
                     YIELANKYGNVKVQNFINGLDLEAKYNSTIVNFVKGDIEINNEYGSLNVSDLDGKIYI
                     DTSYNQNDFRNIQGSLRIDTAYTDNRLEDVYADTEVTGKYGGGRFENLKGDLDLDIRY
                     MGLDLIDIEGAITGSMEYGEISIESLSNDLDVTSRYADLKVWMVEDLRDLNVDVRTRY
                     GNLRSDLSLSKTKEDSTEMINGQIGSGTTDLKIDARHADVELIYEN"
     gene            complement(2460..3221)
                     /locus_tag="VKN64_01730"
     CDS             complement(2460..3221)
                     /locus_tag="VKN64_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA58982.1"
                     /translation="MRSWVSLFKKEIRSMLFTILVSVLLVSAWEIYLITRMDVWPLGL
                     TFGLGFIPFAIFPLIMLVQGYQSFRQEWKNNTIYLLKSLPRSGYEIVSAKLASSSLVY
                     IVLTLYTFGLHMFFHWSQLKILMGQLPEAVNSSYSTRMLVLGIITYIVIGIVPYLLSQ
                     FSYLVSCFYGKFRWLVSIVVFALSHYILVRLGGFIARLFNWLPDIPVDVMWSSPYGEE
                     IATIYLGSGPIVAALLILIGFFMAGSWILEKQLDV"
     gene            complement(3218..3916)
                     /locus_tag="VKN64_01735"
     CDS             complement(3218..3916)
                     /locus_tag="VKN64_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA58983.1"
                     /translation="MTEVAVKVSNLSKKYPNALALDNINVEFPEGQITGLIGPNGSGK
                     STLLKIISGLVVEDSGQVEISRMEARKLMDHIAFLPELNHLYDWFTIKEAINFFDAQY
                     DDFSVEKAEELVDFMNLKMEQKIKTLSKGMVGRLKLILVLARRAPLLILDEPLAGIDP
                     NSRARILESLISEYEADFQSIIISTHEVVEAERFFDHVVFLENGKAILKGNTDDLRAE
                     RNKSVQELVREVFK"
     gene            complement(3909..4286)
                     /locus_tag="VKN64_01740"
     CDS             complement(3909..4286)
                     /locus_tag="VKN64_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMA58984.1"
                     /translation="MNIDFNASKPIYEQVIEEIKKQIARGEISPGDKLPSQRELAKDI
                     QVNPNTIQRAYREMEILDLVETKRGRGTFIKDDDKVIKEINKQMAQSAVQNFIDEMVS
                     IGFEKQEIIDLLKREFERRIKND"
     gene            complement(4456..5331)
                     /locus_tag="VKN64_01745"
     CDS             complement(4456..5331)
                     /locus_tag="VKN64_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMA58985.1"
                     /translation="MREEVISLENGIELNVKYNQKDKPTVLFLHFGSGNLHMWSGVIP
                     HFKERYRVILPDLRGHGQSSKPKTGYHIEDMAEDIELLLDKLNVDRYYIIGSSLGAEV
                     AAVLAANNPKKVIATVNEGALYNEFGEYGLQAGNQKEMEEKIEEKIQEVEKRKEETYK
                     TKREYIETQARFFQRAGLWNEYFEEYIKNNVCKNKEGRYTSCYPIYVSKKYMKNYYHF
                     KFEKYYKKMNCSILFLPSKSEWNDEKIQNSTEAFGKMVGNYEIKILQGFVHAYGWMKM
                     PSQAAEVVLDFLQKH"
     gene            5435..6199
                     /locus_tag="VKN64_01750"
     CDS             5435..6199
                     /locus_tag="VKN64_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58986.1"
                     /translation="MLRNKLFTILLILVLLFSLKLTASAELDFDFFIKLFSVEKDELD
                     YFAKFDISNDNLSTILYFYSSADRTLTRDQFRYLMKHRYNWRELSIYFGLPPVMFDDE
                     VIRLRRPNRERMQVPLDRKRYKDRYKTKNIEEKVELNPGKYEYYYRNKIRDIEEKIEV
                     KQNKYEYYYKSNQMEEKLSVHMVTNKYEYYFKNFYTGQEIKKEGRGRPIDSEKLYQEL
                     RERYKEDDGYKDDEDDDENEDEDNNINFKFKIEFNF"
     gene            6334..7560
                     /locus_tag="VKN64_01755"
     CDS             6334..7560
                     /locus_tag="VKN64_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA58987.1"
                     /translation="MTTLSETISKNKESLKIFAAIGLCLLGDAMLYTVLPTRVELFAF
                     SGAQLGIILAANRVIRLFSNFWAADLYKRWGRNIPFKIAVILSIFITFSYGYFELFWQ
                     FLILRLLWGAVYSLLRLKALLIIFDSGLPENMQGKLSGIFRSMSRSGYMVGMILGGYL
                     TDLFSLSGSAYLLAGLTGLSLFFIFRKDPEKEFNKSGKEEFEPVPKTNLLKLLTSFKM
                     AILMFMGFALNFTGLGLINSSYGLYLKNIFGESLMIFGIAVGIATLNGLILSTQSLFE
                     LILSPWLGNTADKYSTHKLLDYAFYLHAVLMALLVFFKNGFVAIITPLFLFINTAFII
                     IILYIEVSKYQKSSVSDRMAGFTTAVDLGAAFGPLTLIFLDVGLTLSLLYLISASLLL
                     TAGVLYRVHFKKTQVS"
     gene            complement(7620..>7937)
                     /locus_tag="VKN64_01760"
     CDS             complement(7620..>7937)
                     /locus_tag="VKN64_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HMA58988.1"
                     /translation="LLIIPGIIKYYAYRLVPYLLAENPDLGHKRAIQLSQDMMKDEKL
                     KVFILDLSFLGWFILGALFFGIGVLFVQPYYDATNAELYIKLRVKALNNNLTTEEELR
                     MNS"
BASE COUNT         2428 a         1507 c         1183 g         2819 t
ORIGIN      
        1 ttttgtactc aaagctacaa ttaaaggttt aatggcaaag ataattaacc atcccattgc
       61 caagtacata attacactta ttatttttaa cttgtttata aaaaagactt taaatattat
      121 acctaataag gctatcgacc agattgtaat taaaattgtc cagcctaatt tacctcttaa
      181 agcaaccatt gaaatcggtg tataggtacc tgcaattaaa ataaatatgg aagcatggtc
      241 gagttttcgg aggtgtattt tagcactctt tatgggaata ctgtggtata aagtcgaaga
      301 tagataaagt atgataagag aagcaccata tactatataa cttaccaacc catagatatc
      361 agtcgtctta taatgagtta caattaaaaa taccagggcg aaaattccaa aagcaatacc
      421 gagcccatgg gttatagcat tggcaatttc ttctttttta ctgtatttaa ttaaatcccc
      481 cataaaaata ccccctttta ctttctttta ttgttattaa tattattata acaaattttt
      541 acttatttat ataatgaaaa ggctagttat ttttaatata ttaaatcaaa tttcttttag
      601 ataatcaaca tctttaactt ttttgttata gtaatctata acttcttgat acctgaaaca
      661 atcaactaga tgatcattaa caatgcctac cgcctgtaaa aaagcgtaaa tagtaacaga
      721 accaacaaat ttaaaacctt ttttctttaa atttttagct atagtttttg ataattcagt
      781 ttcagctggt acttcggata aggtttccga cttatttata actggcgtaa agacagtaaa
      841 cgaccagagg tatcggctga aactaccata ttcctcttga attttcaaga cacgttcagc
      901 attatttata gaagcatcaa tctttagttt atttctgata atacctttat tgtttaaaag
      961 ttcagttctt tttgcttctt cataagatgc tattttttca atatcataat tatcataggc
     1021 ttctttataa ttgtctcttc tttttaatat ggttgaccag cttaaaccgg cctgagcagt
     1081 ttctaagatt aaaaattcaa atagaatttt atcatcaaaa actggaactc cccattcttt
     1141 atcatggtat ttgatataca tctcactgcc ttcagcccaa tcacatctat tcactctatt
     1201 cacctctata atttaattta tatttgtatt attaaagttc aataatatat ttaaaaaccc
     1261 tttatagtat gtaagcccat ccttccggat gggctttaat ctaaattaat tttcatatat
     1321 taattcaaca tcggcgtgtc tagcatcgat ctttaaatca gttgtaccag aaccaatttg
     1381 accatttatc atttcggtac tgtcttcttt tgtcttggag agacttaaat cagatcttaa
     1441 attaccatat ctggttctta catcaacatt taaatctctt aagtcttcta ccatccagac
     1501 ttttaaatca gcatacctgg aagttacatc taaatcattg cttaaacttt caattgagat
     1561 ttctccatat tccatactac cagttatagc accttctata tcaattaggt ctaaccccat
     1621 atacctaata tctaaatcaa ggtcgccttt tagattttca aacctacccc cgccatattt
     1681 accagttacc tctgtatctg catatacatc ttcaagacga ttatcggtat aggcggtatc
     1741 aattcttaat gaaccctgaa tatttctaaa atcattttga ttataggaag tatcaatata
     1801 tatttttcca tctaaatcag atacatttaa acttccatat tcattattga tttctatatc
     1861 tccttttaca aaattaacta ttgttgagtt atactttgct tctaaatcaa gaccgttaat
     1921 aaagttttgt acctttacat tgccgtactt gttggcaagc tcaatatata aatccttggg
     1981 ggctaatata tcaaagtcta cttcaaatgc cctaatattg gccggttttg gcctcggggc
     2041 ctggacctca agctcaaatg taccattggg ttctccatat aatataccta ttttctctaa
     2101 aaattcttcg gccgcttctt tgctgcttga atatacctta acttcataag taacctcaat
     2161 agtatcttta tcatgcccct gtatatcaac tgaaccgacc gggttgtcta atataaagtt
     2221 ctccaaatca gcagcattat aactaaaact tgattttctt accgcttccg ggcttcttct
     2281 ttcatcattt aaggctacat tgggcatatt acttatattc ttaattgact ctgtttcaaa
     2341 ctgcataaag taatctgcaa ttacatcttc atcatttatt ctgaaatcaa tgaccatgat
     2401 ggcgaccaaa ccgatcagaa tcaatgctat tatctttttc ctttgcattt atatcactcc
     2461 taaacatcta attgtttttc taatatccag gatcctgcca taaagaatcc gattaatatt
     2521 agaagtgctg ctacaatagg gccgctgcct aaatatatgg tggctatttc ttcgccataa
     2581 ggacttgacc acattacatc aaccggtata tcgggcagcc agttgaataa tcgggcgata
     2641 aatccgccta atcttaccaa tatataatga cttaaagcaa atactactat actgactaac
     2701 catctgaact taccgtaaaa acagctgacc agataagaga actgacttaa aagatatggt
     2761 acaataccaa ttactatata agttataatt cctaatacca gcatccgggt tgaataactg
     2821 ctgtttactg cttccggcag ctgtcccatt aaaatcttca gctgactcca gtgaaaaaac
     2881 atgtgcagtc caaaggtata aagtgttaat actatatata cgagggaact cgaagctagc
     2941 ttagctgata ctatttcata accactgcgt ggtaatgatt ttaataaata gatcgtattg
     3001 tttttccatt cctgtctaaa actctgataa ccctgaacta acatgattaa ggggaaaatc
     3061 gcaaaaggta taaatcccag tccaaatgtc aatcctaaag gccagacatc cattcttgtt
     3121 attaaatata tctcccaggc tgatacaagc agtactgaga ccagtatggt gaataacatg
     3181 cttctaattt cttttttaaa taatgatacc cagcttctca tttgaatacc tcccttacaa
     3241 gttcctgtac agatttattt ctttcggctc ttaggtcatc tgtattacct tttaagatcg
     3301 ccttaccatt ttctaaaaat actacatgat caaaaaatct ttccgcttca acaacttcat
     3361 gagtagaaat tattattgac tgaaaatcag cttcgtattc actgattaaa ctttctaaaa
     3421 ttctcgccct tgaattgggg tcgattcctg ctaaaggctc atcaagtatt aaaaggggag
     3481 cccttcttgc taggaccagg attaatttta atctgcctac cattcctttt gaaagggtct
     3541 ttattttttg ttccatcttt aagttcataa aatcaactaa ttcttctgcc ttttcaacac
     3601 taaaatcatc atactgtgcg tcaaagaaat taatagcctc ctttattgta aaccagtcat
     3661 aaaggtggtt taattcaggt aagaacgcaa tatggtccat taactttctg gcttccattc
     3721 ttgatatttc tacctggccg gaatcttcta ctaccaaacc ggatattatt tttaataagg
     3781 tagatttacc gctcccattc ggtccaatta aacctgtgat ctgtccctcg ggaaattcaa
     3841 catttatatt atctaaagca agtgcgtttg gatacttctt tgataggtta cttactttta
     3901 cggcaacttc agtcattttt tatcctcctc tcgaactctc tttttagaag atcaataatt
     3961 tcttgttttt caaaaccgat tgaaaccatc tcatcaataa aattttggac ggctgattga
     4021 gccatctgtt tgttaatctc tttaatcact ttatcatcat cctttataaa agttcctctg
     4081 cctcttttag tttcgactaa gtctaaaatc tccatctccc tgtaggcccg ctgtattgta
     4141 tttggattaa cctggatatc tttggccagt tccctctgtg agggaagttt atcaccagga
     4201 ctaatttctc cgcgagcgat ctgtttcttt atttcttcta taacctgttc atagataggt
     4261 ttggacgcat tgaaatctat attcatcttc gtcctccttt cacattgttt tagtgcacta
     4321 cttatctaat gcactattat tataatatta tttccttatg aatgtcaagt acttttttat
     4381 attttttgca aaaaaataag cacaggagtt gtaatccctg tgctgacaac gtttatattc
     4441 ctatttaatt tttaattaat gcttctgcaa gaagtccaag actacttcag ctgcctgact
     4501 gggcattttc atccagccgt aagcatgcac aaacccctgc agtattttaa tttcataatt
     4561 accgaccatt ttaccaaatg cttctgttga attttgtatc ttctcatcat tccactcact
     4621 tttactgggt aaaaatagga tggaacagtt catcttctta taatactttt caaatttgaa
     4681 gtggtaatag tttttcatgt acttttttga tacataaatt ggataacagg aagtatatct
     4741 cccctcttta ttcttacaaa cattattttt tatgtactct tcaaaatatt cattccataa
     4801 gcctgccctc tggaaaaaac gggcctgagt ttctatgtat tctcttttgg ttttataggt
     4861 ttcttctttt cttttttcca cttcctggat cttttcttca attttctctt ccatctcctt
     4921 ttggttccct gcctgcagtc catattctcc aaattcattg tagagggcac cttcattaac
     4981 agtagctatc acttttttag gattgttagc tgccaataca gctgccactt cagcccctaa
     5041 ggaactgcct attatgtaat atctatctac attaagcttg tctaataaaa gttcgatatc
     5101 ttcagccata tcttcgatgt ggtaaccggt tttgggtttg gaactttgcc catgtcccct
     5161 taaatccggt aaaattaccc ggtatctttc tttaaagtgg gggatgactc cactccacat
     5221 atgtagattg ccagagccaa agtgtaaaaa aagtactgtc ggtttatctt tttggttata
     5281 cttaacattc aactctatcc cgttttctaa acttatcact tcttctctca ttaattatct
     5341 ccccttgtta agtatgcttt aactcattga tatggtgtta tattcagtat ataataatat
     5401 atagagaaaa gaaaatgaaa agaaggtgaa gttcatgctt agaaataaat tatttactat
     5461 attattgata ttagtattac tattttcatt aaagctcact gcttcagctg agttagactt
     5521 tgattttttt ataaaattat ttagtgttga aaaggatgaa ttagactact ttgctaagtt
     5581 tgatatttct aatgacaact taagtacaat tctttatttt tattccagcg cagaccgaac
     5641 tctcaccaga gatcaatttc ggtacctgat gaaacaccgc tataactggc gtgaactaag
     5701 catttatttc ggtttgcccc cggtaatgtt cgatgatgaa gtcatcaggt taagaagacc
     5761 aaatcgagag agaatgcagg ttcccttgga tagaaaaagg tataaggata gatataagac
     5821 caaaaatatc gaagaaaaag tggagttgaa ccccggcaaa tatgaatact attacagaaa
     5881 taagataaga gatattgaag aaaaaatcga agttaagcag aataaatatg aatattatta
     5941 taaatccaat caaatggaag aaaaattgtc ggttcacatg gtgactaata aatatgaata
     6001 ctactttaaa aacttctata ctggacagga aattaaaaaa gaaggccgcg ggcgccccat
     6061 tgattctgaa aaattatacc aggaattgag agaaagatat aaagaagatg atggttacaa
     6121 ggatgatgaa gatgatgatg aaaacgaaga tgaagataac aatataaatt ttaaattcaa
     6181 aatagaattc aacttctaat tgaaaaatat cttttttcgg ccggagtagt tagttctcct
     6241 atattattaa tatgtatttg gaggactaat gctccggcct atttaattaa tttattagaa
     6301 ttcatatatt ttttagataa ggggggaacc tacttgacta ccctatctga gacaatctca
     6361 aaaaataaag agtccttaaa aatatttgca gccatcggat tatgtctttt aggagacgcc
     6421 atgctctata ctgtcttacc gactagagtg gaactatttg ccttcagcgg ggcccagtta
     6481 ggtatcatat tagctgccaa tagagttatt cgactctttt ctaacttttg ggcggccgat
     6541 ttatataaga ggtggggcag gaatatccct tttaaaatag cggttatcct ctctatattt
     6601 attacctttt cctatggtta ctttgaactt ttctggcagt ttttaatatt acgtttactc
     6661 tggggggctg tctactccct gcttaggtta aaagcgttac ttattatctt tgattcaggt
     6721 ctgcctgaaa atatgcaggg taaactatca gggattttta ggtcgatgtc ccgttcgggt
     6781 tatatggtag gtatgatcct gggcggctat ctcacagatt tatttagtct gagtggaagt
     6841 gcttatcttc tagccggctt gactggactc tccctgtttt ttatctttag gaaggatcct
     6901 gagaaagaat ttaataaatc aggaaaagaa gagtttgaac ctgttcccaa aacaaattta
     6961 ctaaagttgt taaccagttt taagatggct attttaatgt ttatgggatt tgcccttaac
     7021 ttcaccggcc tgggcttaat taattccagt tacggccttt atttgaaaaa tatttttgga
     7081 gaaagcctca tgatcttcgg tattgcagtt ggtattgcta ccttaaatgg tctaatactt
     7141 tctacccagt ccttatttga actaatatta agtccctggc tgggtaatac tgctgataag
     7201 tactcaaccc ataagttgct ggactacgca ttttatttac atgcagtatt aatggcttta
     7261 ttagtatttt ttaaaaatgg atttgttgcc attattactc ctttattttt atttattaat
     7321 actgccttta ttataattat cctttatatc gaggtcagta aataccagaa atcctctgtc
     7381 tcagatagaa tggccgggtt tacaactgca gttgatcttg gagccgcttt tggaccctta
     7441 actttaatat ttttagatgt gggattaact ctctcccttc tctacttaat aagcgcatct
     7501 ctactattaa cagcaggggt attatataga gttcatttta aaaagacaca ggtttcttaa
     7561 taaaaaatta agggcagata ccttatctgc ccgtttgtta cttatatatt attaactttt
     7621 taactgttca tcctcaattc ttcctccgtt gtcagattat tatttaaagc ctttaccctt
     7681 aattttatat ataattctgc gtttgtcgca tcataatagg gttgtacaaa taatacccca
     7741 attccgaaaa agagagcgcc taagataaac caacccagaa aagacagatc taagataaag
     7801 acctttaact tctcatcctt catcatatct tggctaagtt ggatagccct tttatggcct
     7861 aaatcgggat tttccgctaa aagataagga actaacctgt atgcgtagta tttaattata
     7921 cccgggataa ttaataa
//
LOCUS       DATGCJ010000039         3809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_362336, whole genome shotgun sequence.
ACCESSION   DATGCJ010000039 DATGCJ010000000
VERSION     DATGCJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362336"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2219
                     /locus_tag="VKN64_01765"
     CDS             <1..2219
                     /locus_tag="VKN64_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58989.1"
                     /translation="GTLAPGESETVNGSYTIVQSDLDNGSVTNTAEVEGEDPEGTIVE
                     DFDSAEVMAEINPDISINKEADPNTYSEVGEVITYTIKVTNTGNVTLYDVEVNDPIAT
                     IYNVGTLYPGGEETINMTMTIISEHLDEEILENTAIASGMDAYGTVVNDTDTAVIEAE
                     IVSEPSISITKEPFPTTYNSVGQEIEYTITVTNIGNVELADVTVIDDLLGINDNLGTL
                     EPGDFVVVTESYYITANDIDRGYVYNLAKVTGNEIVIGKSGDQVSDEAEAEITAEQNP
                     SIAIEKVANPLVYSEVGETISYEIKVTNDGNVTLTEVRVTDPTLGLDYLVGEMAPGDE
                     DNLPTQTYNITQADIDAGAVENTATAEGKDPSDETVSDSDDAVVTAEQSPSIAIEKVA
                     SPLVYSEVGETISYEIKVTNDGNVTLTDVMVTDPTLGLNYSVGEMVPGDEDNLPTQTY
                     NITQADIDAGSVENTATAEGKDPADEPVSDSDDAVVTAEQNPSIAIEKVANPLVYSEV
                     GETISYEIKVTNDGNVTLTEVRVTDPTLGLDYLVGEMAPGDEDNLPTQTYNITQADID
                     VGSVENTANAEGKDPSDEPVSDSDDAVVTAEYVPGKSETAWAYHEDYAKTFIELGISN
                     NWGWFNGPLSPKGEYELELWAGAGQNDLSKGTLVGTVIVSFDEETVKYIVDNGFYLRE
                     VQLYIGNEPTPTASPGLLGYNDEDVNSQEYTIYFSYFSEVNFNEDIYIAAHAVVEY"
     gene            2363..3265
                     /locus_tag="VKN64_01770"
     CDS             2363..3265
                     /locus_tag="VKN64_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58990.1"
                     /translation="MLRRKIPVILLLVIFTFTMGGCDIFNADTVSNVKTGEGLSISQV
                     QPLDQVYVDPGTVETDVIDILNKKRNKVEIVLSDDSKVMAEVAWEGSIGNYNSEELGT
                     YQFGGTALYNGLSADASINVVVDDGYTLTLAIKGEGEIRDKENNIILSAPKNIKEIRC
                     EPGTILQYTAVPHGNWLFMSWNGEHNIKESTVEITMDEHKELQATFGNILIFDHEIFF
                     ESWYNWGAKGKLENLLGYKIESIEIHVEIYLDNGSEVITTSGKITDIADREIVDWYIG
                     GDKFADLRNATDYKIILRNIQLGE"
BASE COUNT         1340 a          560 c          788 g         1121 t
ORIGIN      
        1 taggaaccct tgcacctggt gagagtgaaa ctgtaaacgg cagttatacg atagttcagt
       61 ctgacctaga taacggtagt gtgacaaata ccgcagaagt ggaaggtgaa gatcctgaag
      121 gaactattgt agaagatttc gattctgcag aagtaatggc cgaaattaat cctgatattt
      181 caataaacaa agaagctgat cctaacactt acagtgaagt tggtgaagtg atcacttata
      241 caataaaagt aaccaacaca ggtaatgtga ctctatatga tgtggaggta aatgatccca
      301 tagcaactat ttataatgtg ggtaccctat atccaggtgg agaagaaact ataaatatga
      361 ccatgaccat tatatcggaa catttggatg aggagattct tgaaaataca gcaattgcat
      421 caggtatgga tgcctacgga actgtagtga atgatactga tacagccgtg atagaagctg
      481 agattgtatc tgagccttca atttcaataa ctaaagaacc ctttcccaca acttataata
      541 gtgtagggca ggaaatagag tataccatta cagtgaccaa cattggtaat gtagaacttg
      601 cagatgttac tgtgattgat gatctattag gtataaatga taatttagga accttagaac
      661 ctggtgattt tgtagttgtt acagaaagtt attatattac cgctaatgat atagacagag
      721 gttatgtata taatttggca aaggtaacag gaaacgaaat agttataggt aaaagtggtg
      781 accaagttag cgatgaagct gaggcagaaa taacagcaga acagaatccc agtatagcaa
      841 tagaaaaggt agctaatcca ttagtctaca gtgaagttgg tgagaccatc agctacgaaa
      901 taaaagtaac aaatgatggt aatgtaaccc tcaccgaagt aagggtaacc gatcccacct
      961 taggattgga ttatttagta ggagagatgg cccccggtga cgaggataac ctaccgacac
     1021 aaacctataa tataactcag gcagatatag atgccggtgc tgtagagaac acagcgaccg
     1081 cggaaggtaa agacccgtcc gatgagactg tatcagactc ggatgatgct gtggtaaccg
     1141 cagaacagag tcccagtata gcaattgaga aggtagctag tccattagtc tacagtgaag
     1201 ttggtgaaac catcagttat gaaataaaag taacaaatga tggtaatgta actctcaccg
     1261 atgtaatggt aaccgatccc accttaggat tgaattattc agtaggtgag atggtccctg
     1321 gtgacgagga caacttaccg acacaaacct ataatataac tcaagcagat atagatgccg
     1381 gttctgtaga gaacacagcg accgcggaag gtaaagaccc ggccgatgag ccggtgtcag
     1441 actcggatga tgcagtggta acagcagaac agaatcccag tatagcaata gaaaaggtag
     1501 ctaatccatt agtctacagt gaagttggtg agaccatcag ctatgaaata aaagtaacaa
     1561 atgatggtaa tgtaaccctt accgaagtaa gggtaaccga tcccaccttg ggattggatt
     1621 atttagtagg agagatggcc cccggtgacg aggacaacct accgacacag acctataata
     1681 taactcaagc agatatagat gtcggttctg tagagaacac agcaaatgcg gaaggtaaag
     1741 acccgtccga tgagccggta tcagactcgg atgatgcggt ggtaacagca gaatatgtgc
     1801 ctggtaagtc agaaactgcc tgggcttatc atgaagatta tgcgaagacc tttatagaac
     1861 taggtatttc aaataattgg ggctggttca atggaccact gtcaccaaaa ggagaatatg
     1921 aattagaact ttgggctgga gctggtcaaa atgatttaag taagggaaca ctggttggaa
     1981 ctgtaatagt atcttttgat gaagaaacgg tcaagtatat tgtggataat ggtttttatc
     2041 taagagaggt ccaattatat ataggtaatg agcccacacc aacagctagt cctggtttat
     2101 taggatataa tgatgaagat gtcaattcac aggaatatac tatttatttc agttatttct
     2161 ccgaggttaa ttttaatgaa gatatttata ttgctgctca cgctgtagtt gaatactaat
     2221 aatagatata tttatttcgg tggctgccga tcatttagta gtaacggcag ccccggaatt
     2281 tacaattcac ttaacatgtt ctaattaata ttatatttta aaaaaacaaa aaaacagcta
     2341 aaaaaaataa ggaggattaa aaatgttaag gagaaaaatt ccagtgatac ttttattagt
     2401 gatttttacc ttcactatgg ggggttgtga tatttttaat gcagatactg tgagtaacgt
     2461 aaaaacagga gagggtttat ccattagcca agttcaaccc ctggaccaag tatatgttga
     2521 tcctggaact gttgaaacag atgtcattga tatattgaat aaaaaaagaa ataaggttga
     2581 aatagtttta agtgatgata gtaaagtaat ggctgaagtt gcctgggaag gttcaattgg
     2641 aaattataat tctgaagaac tgggtactta tcaatttggg ggtacagctc tctataatgg
     2701 tttaagtgca gatgcttcta ttaatgttgt ggtggatgat ggttatacat taactttagc
     2761 aattaaagga gaaggagaaa tccgggataa agaaaataat attatattat ctgctcctaa
     2821 aaacataaaa gaaattagat gtgaaccagg aaccatatta cagtacactg cggttcccca
     2881 tggaaattgg ttgtttatga gttggaatgg ggagcataat attaaagaat ctacagttga
     2941 aattactatg gatgaacata aagagttgca agcgactttt ggtaatattt tgatttttga
     3001 tcatgaaatt ttctttgaga gttggtataa ttggggtgca aaaggaaaat tggaaaattt
     3061 acttggctat aaaatagaat caattgaaat tcatgtagaa atatatttag acaatggtag
     3121 tgaagtgatt actactagcg gcaaaataac ggatattgct gacagagaaa tagtggattg
     3181 gtatattggt ggcgataaat ttgctgacct tcgaaatgcc actgattaca aaattatatt
     3241 aaggaatatt caattagggg aatagcaaaa tactgaattc aaaaattaaa ttaacttctt
     3301 gaatacaatt cataataaat ctagacagag actttttgct tctcttaaac aaaggctata
     3361 catatagatc attttaatat ttcttctaat tcttaaccat tttaaaaata ttatactttg
     3421 attggacatg ataaaccatt aatatattgt tattattatc aattgctaaa tgaatcatct
     3481 gattattaat aatttttttc aacttattaa ttgaatcatt ccaattcgtt tttatatcta
     3541 attatctaac tttttaaata ataatttcat attatcaaaa acctattaat tagcttagcc
     3601 tttaaggctg ggcttttttt caggatatgt cttttttaca ctaatattct gattttgctt
     3661 actacctacc ttattatttg tcttagacta agcctttaaa gttaaattaa tttaagtaat
     3721 aataaaaatc ttaacaatag aaggggaatt ataatacaaa gaaggttcat ttagatattc
     3781 aattatttta tgatattttt tgtatctta
//
LOCUS       DATGCJ010000040         7015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_183647, whole genome shotgun sequence.
ACCESSION   DATGCJ010000040 DATGCJ010000000
VERSION     DATGCJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183647"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VKN64_01775"
     CDS             complement(<1..330)
                     /locus_tag="VKN64_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMA58991.1"
                     /translation="MIYKILLTLDYLAIFYFFLVNTTYIILNIIAFFSIRKKRLKKKI
                     QDIDKPFSSEFYKPLSIIVPAYNEEKTIVSNLESILELQYPEFEVIVVNDGSSDNTLQ
                     RLFENFNL"
     gene            complement(320..1420)
                     /locus_tag="VKN64_01780"
     CDS             complement(320..1420)
                     /locus_tag="VKN64_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58992.1"
                     /translation="MTNYLYISLYLFSFLSLLIFLIIIIYHKMQNLKKKKITEYQEKY
                     YPIIDDYINKQVSLKKATNMMKDDINYELMVEFLKPLLKKYSGKKYEKLKILIQQAGI
                     NDYYLRKLNSNKQKEKLKAASIIGKLEDERALNKLKQMLESQENLVIITASWALSDIG
                     NMESLDKIINVLFNKTNMTYEAITELLVNFDEDICDKLLDFINNYLEDNTYFTTNFAV
                     AEFKLLSLFIDIFGYFRYEQSLPVMKRLLKINLHEEVIIHIFKSLVKIGEPIEIDFSN
                     YLSHKNWVIRSQTAKYLSIIKINIFIDKLKELLSDDNWWVRYYAAKAIWELGHIDLLV
                     DIIIKNKPGANICDYILAQNNYNYVLGDKNDI"
     gene            complement(1501..2292)
                     /locus_tag="VKN64_01785"
     CDS             complement(1501..2292)
                     /locus_tag="VKN64_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMA58993.1"
                     /translation="MQRFTTLLLGLLILLTVLFGPVISETNAEEVDIKKGLVSYLKLN
                     DTSSKIIDTVGQIQSENNGATFREDNIQFDGEDDFINLTNFNLKNLGQGSISLWFKVE
                     DIPTQDAIRPILYYGAKDPCTNMPDASNQGLIIEVAHDPMHFQSKNLYFTIFADGCEY
                     PSFCFDSREPMQEKTWYHFVAVVGEDFNTGYLNGQEITNRRYNFGNARDSEFFEDAVK
                     DEAFWIGKGYWDAKPMYFKGNIKNIRIYNRSLNKDEVQYIYQNDL"
     gene            2396..2632
                     /locus_tag="VKN64_01790"
     CDS             2396..2632
                     /locus_tag="VKN64_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58994.1"
                     /translation="MLTIKCSKCKTKLFKYKKIGEGKVIRCWKSKITRVYETEVNNGK
                     LLCGNCGNVIGEVDYKKVDMISDAFTYTGTKVSK"
     gene            complement(2635..3153)
                     /locus_tag="VKN64_01795"
     CDS             complement(2635..3153)
                     /locus_tag="VKN64_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA58995.1"
                     /translation="MEILTERLKLVPLTAEDIEKSLESLSNFYREKGYKNKQEHLSNL
                     MYKIYNIKLVNMKNDPDNYLFYTYWLIIKRKTNQIIGRIGFKNIPDEQGEIEIGYGIS
                     IEFRSNGYMTEALNAMIKWGFKQKLLPINKIVAKTKKDNIPSQKVLKKAGFDFKYKED
                     EFMCWKKDRISS"
     gene            3417..4601
                     /locus_tag="VKN64_01800"
     CDS             3417..4601
                     /locus_tag="VKN64_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58996.1"
                     /translation="MSNIAFGVITNNVDKFYPYCDFILNAQEYGHQIDKLFICYSHRL
                     NEKKVKRMRKYVDIELLKINNNSKLKNQLLDLGLTEFEVKKLIQSRDLKKHDLVPYGK
                     RRNIVLLAALLSEPKIDYLMFFDTDVKPYVLADKEGNKIEIDFVGNHLKYLKEEDVVV
                     TTSDYTGYYIIPSMYFDGLEELLIGLQKEKAYEFVKSEGHNLITASEKDILNAKGTNK
                     ILGGNHAIDLNKYELLAPYYSTTYHYRQDLVLGRGEDTLLGQVIPDMGGKIIDIETKI
                     FHNTFDNYPEVPNIHNTDIRERFYCACLGWLGRNPFLNWYLKNKGEMAPEKFDKNIKE
                     LRENLVEGSYQFADNYNSPKFKDLPEAFSVAYSQLDHMVEDYQQLMDIWDKFIAGLKQ
                     RR"
     gene            4608..5792
                     /locus_tag="VKN64_01805"
     CDS             4608..5792
                     /locus_tag="VKN64_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA58997.1"
                     /translation="MRVLLVNHFPLEGSGSGIYTKNIAKRLIERGHEVEAIVVDKYKN
                     RGNGFPVKTIINYDFPCFTTHPHSNNQFYHLTNDEMDNYLEAFIKTIDKEVNSFEPDI
                     IHCQHIWVAPFAASKTELPYIITAHGTDIKGFKKDKRYHQIAIKAAEGAEKIITISKY
                     IHDSVKKHFPVKEEKLRMILNGFDENIFHPKNLNKKNVLSNYINKDLSGLDDINLINF
                     TGKLTDFKGADLLLKAAEIYEKELDNVVTLIIGDGELRDQLEQLKEELNLNSIHFLGN
                     LPQETVAELYSIADITVVPSRVEPFGLVALESLACGTPVIASKAGGLKDFINNEVGRF
                     FPMDDYQALASEIIGALKNNEKDEKGERASDYALTNYSWNRVIDELLEVYNEVVEEGK
                     NR"
     gene            5789..>7015
                     /locus_tag="VKN64_01810"
     CDS             5789..>7015
                     /locus_tag="VKN64_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA58998.1"
                     /translation="MKKVMVVPTYWGRDSETGWQLGDDIYDHPTPIDKEGTLPRFFKS
                     LQILADKEFELVIILATTTPEIEKQASEKVKKIIKEYSPKEVTTYLFSARTLNKIKSF
                     YNEKSSLPLRLFTLRGYSNIRNMCIYGGYLAGGDVVILIDDDEIFEDEIFIKKVSEFI
                     GGRFYGQTVDGIAGYYINENGEYYDDVNIEPWMTYWNRFGSKSKAFDKIIGSSPRLKI
                     TPFAFGGLMVIHKNLYKIVPFDPNLTRGEDIDYLINARMFGFNFFLDNKLSILHLPPE
                     KPHPVWQRFRQDMYRFYYEKKKLETQLEKSNMQLVSAEDFDPYPGEFMKKDLEDKIFK
                     ANIMLALDYLSDNEIENTKATLKNIYISKYSAEPEYNVFKHYLELQDEWENILNFADD
                     NFPELQDIFKDTLVGET"
BASE COUNT         2463 a          977 c         1143 g         2432 t
ORIGIN      
        1 taagttaaaa ttttcaaata atctttgcaa tgtattatcg ctagatccgt cattaacaac
       61 aattacttca aattccgggt attgtagttc tagaattgat tctaaattag atactattgt
      121 tttttcttcg ttataagcag gcacaataat acttaaaggt ttataaaatt cactgctgaa
      181 aggtttatct atatcttgga ttttcttttt taatcttttt ttacgtattg agaagaaagc
      241 aataatattt aaaattatat aagtagtatt aacaaggaaa aaataaaaaa tggctaaata
      301 atctaaggta agtagaattt tatatatcat ttttgtcccc caatacatag ttataattat
      361 tttgagccag gatgtaatca caaatatttg ctcccggctt gttttttata ataatatcta
      421 caagtaaatc tatatgaccc agttcccata ttgctttggc agcatagtat cttacccacc
      481 agttatcatc agataaaagt tcttttagtt tatcaataaa aatatttatt tttataatac
      541 ttaaatattt agctgtttgg cttctaataa cccaattttt atgtgataaa taattagaaa
      601 agtcaatttc gataggttcc ccaattttga caagggactt aaaaatatgg ataataactt
      661 cctcatgaag atttattttt aataatcttt tcatcacagg taagctttgt tcatatctga
      721 agtaaccaaa tatatctata aataatgata ataatttaaa ctctgccact gcaaaattag
      781 tagtaaaata agtattgtct tctagataat tgttaataaa gtccagtaat ttatcacata
      841 tatcttcatc aaaattaact aatagttctg tgattgcttc ataagtcata ttggttttat
      901 taaatagtac gtttattatt ttatctaaag attccatatt accgatgtct gataaagccc
      961 aactcgcagt tataattacc aagttttctt gcgattctaa catttgcttt aatttgttaa
     1021 gggctctttc atcttctaat ttaccaatta tagaagcagc ttttaatttt tctttctgct
     1081 tatttgaatt taattttcgt agataataat catttatacc tgcttgttgg ataagtattt
     1141 ttaatttttc atacttttta cctgaatact tttttaggag gggctttaaa aattctacca
     1201 ttaattcata atttatatca tccttcatca tatttgtagc tttttttagt gaaacttgct
     1261 tatttatata atcgtcaatg attggataat atttttcttg atattcggta atttttttct
     1321 tttttagatt ttgcatttta tgataaatta ttataattaa aaagatcagc aaggaaagaa
     1381 atgaaaatag gtataaagat atatacaaat aatttgtcat gattaactcc cctgtctagt
     1441 tagatttgtt aaaatatatt aaataaatag ttatgtttac ctgttaaatg ggaaaaataa
     1501 ttataaatca ttttggtaga tatattgaac ctcatcttta ttaagtgacc tattatagat
     1561 acggatgttt tttatatttc ctttaaagta cattggcttt gcatcccaat aaccctttcc
     1621 aatccaaaaa gcctcatctt taaccgcatc ttcaaagaat tcagaatctc ttgcattacc
     1681 aaaattgtat cttctgtttg ttatttcctg tccgtttaaa tagccagtat tgaaatcttc
     1741 acctactact gctacaaagt gataccaggt tttttcctgc attggttctc tagaatcaaa
     1801 acaaaaagaa gggtattcac agccgtcagc aaagattgta aagtataagt tttttgattg
     1861 aaaatgcatg gggtcatggg ctacttcaat aattaaacct tgattagaag catctggcat
     1921 attagtacag ggatcttttg ccccataata aaggatcggt ctaattgcat cttgggtggg
     1981 aatatcttca actttaaacc acaaagatat tgatccctga cctaggtttt ttaaattaaa
     2041 gtttgttaaa ttaataaagt catcttcacc atcaaattga atattgtctt ccctaaaagt
     2101 tgccccatta ttttcacttt gaatttgtcc tacagtatct attattttcg aactagtgtc
     2161 atttaatttt aaatatgata ctaaaccttt ctttatatca acctcttccg cattggtttc
     2221 cgaaattacc ggcccgaaca gcacagtcaa taaaattaac agacctaata ataaggtagt
     2281 aaatctttgc ataattttct cctttcgaga agtagaaatt accaacatta attttaatta
     2341 gcttaaaaat ttgttatact aaattaaatc acatgaggca aatgaggtgt gtaagatgct
     2401 caccataaaa tgcagtaaat gtaagaccaa actgtttaaa tataagaaaa taggagaggg
     2461 caaagttata agatgttgga aaagtaagat tacccgagta tatgaaaccg aagtaaataa
     2521 tggtaaactg ttatgtggca actgtggaaa tgtaattgga gaagtagatt ataaaaaagt
     2581 agatatgata agtgatgcct ttacttatac gggtaccaaa gttagtaaat aactttaaga
     2641 gcttatccta tcttttttcc aacacataaa ctcatcttct ttatatttaa agtcgaatcc
     2701 tgcttttttt aatacttttt gggaaggtat gttatctttt tttgttttag ccactatttt
     2761 attaatagga agcaatttct gtttaaaacc ccatttaatc atagcattaa gggcttctgt
     2821 catatagcca ttagaacgaa attcaatact tattccataa cctatctcta tttcaccttg
     2881 ttcatctggt atatttttaa aaccgattct tccgataatc tgattggttt ttctttttat
     2941 tattaaccaa taagtataaa atagataatt atctggatca tttttcatat ttaccagttt
     3001 aatattatat attttgtaca tcaaattaga taggtgttct tgtttatttt tatagccttt
     3061 ttcacgataa aaattgctta aactttctaa tgatttttct atatcttcag ctgttaaagg
     3121 gactaatttc aatctttcag tcagtatttc cacgttcaac ttcctttcaa tcattgatat
     3181 ttttacaatt attacttagt actattatat tatttaccga aaagaaatac aatgatataa
     3241 aggcaaaatg ttaccattta tggggagttt atttgacatt tatttcgcat atcaggtaaa
     3301 atataaatac aaagaataaa tatataaata ttatataaat atttgttact gcaaccaatt
     3361 cactaattat tttcaaggag tgaattgggt ttttttattt tagggggttg aaaaaaatga
     3421 gtaatattgc ttttggcgta atcacaaata atgttgataa attttaccct tactgtgact
     3481 ttattctcaa tgcacaggag tatggtcatc aaattgataa gttatttatc tgttattcac
     3541 acaggttaaa tgaaaagaaa gtaaaaagaa tgaggaaata tgttgatata gaattgttaa
     3601 agataaataa taatagtaaa ttaaaaaacc aacttcttga tttaggatta actgaatttg
     3661 aagtgaagaa attaattcaa agccgggatt taaaaaaaca tgatctagtc ccctatggta
     3721 aaagaagaaa tatagtatta ttagccgctt tactatctga acctaaaatt gattatttaa
     3781 tgtttttcga tacagatgtt aagccttatg tattggctga caaagagggg aacaaaatag
     3841 aaattgactt tgtgggaaac catcttaaat acttaaagga agaagatgtt gtagttacta
     3901 caagtgacta taccggctat tacataatcc cttctatgta ttttgatggg ttagaagaat
     3961 tattaatagg actgcagaag gaaaaagctt atgaatttgt taaatcagaa gggcataatt
     4021 taattacagc aagtgaaaaa gatattttaa atgcgaaggg gaccaataag atactgggtg
     4081 gtaaccacgc tattgatcta aataaatatg aacttttagc accttattat tctaccactt
     4141 accattatcg tcaggactta gttttgggaa gaggagaaga tacattactt ggtcaggtga
     4201 ttccggatat gggcggcaaa ataattgata ttgaaactaa aatttttcat aacacctttg
     4261 acaattatcc agaagtaccg aacattcaca atacagatat tagggagaga ttttattgtg
     4321 catgtctggg atggttaggg aggaatccct tcttaaattg gtatttaaaa aataaaggag
     4381 aaatggcacc tgaaaaattt gataagaata ttaaggaatt aagagaaaac ttagtagaag
     4441 gtagttatca atttgctgat aattataatt ctcccaaatt caaggactta ccggaagctt
     4501 tttcagttgc atattcacaa ctagaccata tggtagagga ttatcagcag ttaatggata
     4561 tttgggataa atttatcgca ggattaaaac agaggaggta gtttcaaatg agagtacttt
     4621 tggttaatca ctttcccctc gaggggtcag gaagtggtat ttatacaaaa aatattgcaa
     4681 aaaggttaat tgagaggggg catgaagtag aagcgatcgt agtagataag tataaaaatc
     4741 gtggtaatgg atttccggta aaaacaatta ttaattatga ttttccctgt tttactaccc
     4801 atccgcacag caataatcaa ttttatcacc ttactaatga tgaaatggac aattatctgg
     4861 aagcttttat taaaactatt gataaagaag taaatagttt tgaacccgat attatccact
     4921 gtcagcatat ctgggttgca ccttttgcag cttctaaaac tgaactccca tatataatta
     4981 cagcccatgg cacagacata aaaggtttta aaaaggataa aagatatcat cagattgcta
     5041 taaaggcagc agagggggca gaaaagataa tcacaatatc aaaatatatt catgacagtg
     5101 ttaaaaagca tttcccggtc aaagaagaaa aattaagaat gattttaaat ggatttgatg
     5161 agaatatttt tcatccaaaa aatttaaata aaaagaatgt tttaagtaat tatatcaata
     5221 aagatctgtc tggattagat gatataaatt taatcaactt tactggtaag ttaacagatt
     5281 ttaaaggagc tgacctttta ttaaaagcgg ccgaaattta tgaaaaagag cttgataatg
     5341 ttgtaacatt aataattgga gatggtgaac tgagagatca attagaacaa ctaaaagaag
     5401 aattaaattt aaattctata cattttttag gtaacctgcc acaggaaact gtagcagaat
     5461 tatattcaat tgccgatatt acagttgtac catctagagt agaaccgttt gggttggtag
     5521 ctttagaatc tctagcctgc ggtacaccgg ttattgcttc aaaagccggg ggacttaagg
     5581 actttattaa caatgaagtt ggccggtttt tccctatgga tgactatcag gctcttgctt
     5641 ctgaaattat tggggcccta aaaaataacg aaaaagatga aaaaggcgaa agggccagtg
     5701 attatgcctt aactaattat tcctggaaca gggtaattga tgaattatta gaagtatata
     5761 atgaagttgt tgaggaggga aaaaatagat gaaaaaggta atggtagtac ctacttactg
     5821 gggccgtgat tctgagacgg gctggcagtt aggagatgat atatatgatc atcctactcc
     5881 cattgataaa gagggtacat tacctaggtt tttcaagagc ttgcaaatcc ttgcagataa
     5941 agagtttgaa ctagtgatta ttttggcaac tacaactcct gaaatagaaa aacaggccag
     6001 tgaaaaagtt aaaaagataa taaaagagta tagtcctaaa gaagttacca cttacctatt
     6061 ttctgcccgg accttaaata aaataaagtc attttataac gaaaaaagtt cgctacccct
     6121 tcgtttattt actcttaggg gatattctaa tattagaaac atgtgtatct atggtggtta
     6181 tttggccggg ggagatgtgg taatattaat agatgatgac gagatttttg aggatgaaat
     6241 ctttattaag aaagtatcgg aatttatagg tggtagattt tacggtcaga cagttgatgg
     6301 tattgcaggt tattatatca atgaaaatgg agaatattat gatgatgtaa atattgaacc
     6361 ctggatgacc tactggaata gatttggtag taaatcaaaa gcttttgaca aaattatcgg
     6421 tagttcaccc cgtcttaaga taaccccttt tgcatttggg ggactaatgg ttatccataa
     6481 aaatttatat aaaattgtac cctttgatcc caatctaacc aggggtgaag atatagatta
     6541 tttgattaat gcccgcatgt ttggttttaa ctttttttta gataataaac tttccattct
     6601 ccatttacca cctgagaaac cacatccggt ctggcagcga tttagacagg acatgtaccg
     6661 gttttactat gaaaaaaaga agttagaaac tcaattggaa aaatctaata tgcagttagt
     6721 gagtgcagag gactttgacc cttatccggg tgaatttatg aagaaggatt tagaagacaa
     6781 gatcttcaaa gcgaatataa tgttagcttt agattactta agtgataatg aaatagaaaa
     6841 tactaaagca actttaaaaa atatctatat ttccaaatat tcggccgaac cagagtataa
     6901 tgttttcaaa cattatttag aattacagga tgagtgggaa aatattttaa actttgctga
     6961 tgataatttt cctgaactac aggatatttt caaggatacc ctggtaggtg aaaca
//
LOCUS       DATGCJ010000041        35383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_139032, whole genome shotgun sequence.
ACCESSION   DATGCJ010000041 DATGCJ010000000
VERSION     DATGCJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 35383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35383
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139032"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..191
                     /gene="rpmJ"
                     /locus_tag="VKN64_01815"
     CDS             78..191
                     /gene="rpmJ"
                     /locus_tag="VKN64_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HMA58999.1"
                     /translation="MKVRPSVKPICDKCKVIRRNGKVMVICENPKHKQRQG"
     gene            218..604
                     /gene="rpsM"
                     /locus_tag="VKN64_01820"
     CDS             218..604
                     /gene="rpsM"
                     /locus_tag="VKN64_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMA59000.1"
                     /translation="MARIEGVDLPRNKRVEIGLTYIYGIGRSTSQNILDNTGVNPDTR
                     VKDLTEAEISKIRKEIDKYPVEGELRREVRANIKRLKDIGCYRGLRHRRGLPVRGQRT
                     RTNARTRKGPKKTVGAKRSKATKTAK"
     gene            628..1032
                     /gene="rpsK"
                     /locus_tag="VKN64_01825"
     CDS             628..1032
                     /gene="rpsK"
                     /locus_tag="VKN64_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMA59001.1"
                     /translation="MAKSKKKGKKRGRAKKKKINVDSGIVHIKSTFNNTIITVTDKEG
                     NVILWHSAGKAGYQGSRKSTPFAAQIAAEEAAQEAKDLGLKSVEIYVKGPGSGRESAI
                     RSIQATGLDVSLIKDITPIPHNGCRPPKRRRV"
     gene            1074..1706
                     /gene="rpsD"
                     /locus_tag="VKN64_01830"
     CDS             1074..1706
                     /gene="rpsD"
                     /locus_tag="VKN64_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMA59002.1"
                     /translation="MSRYTGSVCKLCRTEGEKLYLKGARCYSDKCAYERKPNSPGEQG
                     TGRRRRKVSEYGSQLREKQKVRRIYGLQENKFHRYFKEAERDNGITGEVFLQLLERRL
                     DSAVYRMGFARSRNEARQFVLHGHIEVNGEKVNIPSYKVEEDDVISVKESSFKKDTFQ
                     EIFDVNANLTPPEWVSVDLEKGEGTVVSLPTREDIEYPVEEHLIVEFYSR"
     gene            1761..2705
                     /locus_tag="VKN64_01835"
     CDS             1761..2705
                     /locus_tag="VKN64_01835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMA59003.1"
                     /translation="MIEIEKPQIERLESSDKYGEFEISPLERGYGITLGNSLRRILLS
                     SLPGAAITSVKIEGVRHEFSSIEGVKEDVTEIILNLKEVVLRYKGEEMQTMNLNVEGK
                     GEVTAGDFRTSGEVEVINKDHHIATLAEGGKIMMEVTVESGRGYVLAEENKEHFDKVI
                     GLIPIDSSFSPIERANFRVENTRVGQVTDYDRLVIEVETNGSITPDDAISLGAKIMVE
                     HLELFINLTDEIEDVEIMVEQEEEEQNKVLETTIEELELSVRSSNCLKRAGINNVGEL
                     IEKTEDDLMNVRNLGKKSLQEIKDKLDELDLSLKEPEV"
     gene            2721..3068
                     /gene="rplQ"
                     /locus_tag="VKN64_01840"
     CDS             2721..3068
                     /gene="rplQ"
                     /locus_tag="VKN64_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMA59004.1"
                     /translation="MAKRKLNKKSSHRKALFKNQLISLFKNEQIKTTLPKAKELRPIA
                     EKMITKAKKNNVNARRQAMGKLNDKEAVEKLFDVIAPRYMERPGGYTKIIKLYPRRGD
                     ASEMALIKLVEEE"
     gene            3102..3917
                     /locus_tag="VKN64_01845"
     CDS             3102..3917
                     /locus_tag="VKN64_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HMA59005.1"
                     /translation="MSLLEVQNVEFKYHGQTEPALKDINFEVEQGDFVSVIGANGSGK
                     STLAKLLNVLLRPDKGNIIVEGQNTRNKEKIWDIRKNVGMVFQNPDNQIVATMVEDDV
                     AFGLENIAIPSSEIRKRVDHALDLVDLTGYETHETHKLSGGQKQRLAIAGIIAMEPSC
                     IVLDEPTAMLDPQGRKEVIDTIKFLNKEKNITIVLITHFMEEASISDKVYVMNQGQIQ
                     SQGPPAEVFKKIDYLRSINLDVPVVMEVANGLKNKGVNIPDILTIDGLVDALC"
     gene            3911..4771
                     /locus_tag="VKN64_01850"
     CDS             3911..4771
                     /locus_tag="VKN64_01850"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HMA59006.1"
                     /translation="MLIELQNVTHIYDQEQSIKALDDIDLKIDEHEFIGIVGHTGSGK
                     TTLVQMFNSLIKPTSGSIYVDGINTTEKKADLKHVRQTVGLVFQYPEHQLFEETIYDD
                     VAFGPRNLKLGKEEVDKRVKVALDLVGLDFEEFKDRSPFNLSGGQQRRVAIAGVLAMR
                     PRVLILDEPSAGLDPQGREKLKALLKRLHKSYNITIILISHRMEEITQLSSRVIVMDH
                     GKIVLNGPPREVFSHKEKLEKLSLELPEITEILFRLRERGCDIRTDIFTVEEAVDEII
                     KVIGSQENVN"
     gene            4761..5552
                     /locus_tag="VKN64_01855"
     CDS             4761..5552
                     /locus_tag="VKN64_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HMA59007.1"
                     /translation="MLTDITIGQYVHGNSIVHILDPRIKIINTIILITSLFLIANFWG
                     FAIFLGFNLFVIYISNLPLTKVLKGLKPILFLVLLTVILHIFMTRGGEVLFQWKFLRI
                     ESEGLYTGTFMASRILLLIMFTSLLTLTTSPLQLTDGIEYILKPLKRIGVPASEISMM
                     MTIALRFIPTLMEEAEKIMKAQKARGADFESGNLIQRAKNLIPLLVPLFISAFRRADE
                     LALAMESRCYQGGKKRTRLHIMQIKRRDVIALIISIALAVVVSFY"
     gene            5563..6300
                     /gene="truA"
                     /locus_tag="VKN64_01860"
     CDS             5563..6300
                     /gene="truA"
                     /locus_tag="VKN64_01860"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMA59008.1"
                     /translation="MRFYKVILEYDGTNYNGWQRQKNTDTTIQQIVEEKLSIINKSFV
                     LATSAGRTDAGVHAKRQVVNFKLDVDVPVRRIPFALNSLLPKDIICTDSEEVDEDFHA
                     RHDAKGKLYRYRLSNSRFPSVFTRNYVYNIRSKIDFKKIKKAAPLLVGTHDFASFQNS
                     GSDMEDTVRTIEKIDIYQVEDEYHFDIKGSGFLYNMVRIIMGTFIEIGQDKIKLDDLE
                     DIMESKNRRRAGFTAPAKGLTLMDVYY"
     gene            6395..6823
                     /gene="rplM"
                     /locus_tag="VKN64_01865"
     CDS             6395..6823
                     /gene="rplM"
                     /locus_tag="VKN64_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMA59009.1"
                     /translation="MTTYMAKPDEVDKNWYVVDAEDKTLGRLASRIATVLRGKHKPEF
                     TPHVDTGDFVIVVNAGKVKLTGNKWDQKVHYKHSRYPGGLKTMTYGDLKKHNPKEIVR
                     HAVDGMVPNNKLGKKMMKKLKIYAGPDHPHQAQQPEELEV"
     gene            6850..7245
                     /gene="rpsI"
                     /locus_tag="VKN64_01870"
     CDS             6850..7245
                     /gene="rpsI"
                     /locus_tag="VKN64_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMA59010.1"
                     /translation="MAAEVQYWGTGRRKTAVARVRLVPGDGNILVNDRDVQDYFNRKS
                     LIQDLMSPLELTNTTDNLDVLVNVNGGGLSGQAGAVRLGIARALLKVDGDYRKPLKDA
                     GYLTRDSRMKERRKYGHKKARKSPQFSKR"
     gene            7389..8207
                     /gene="amrB"
                     /locus_tag="VKN64_01875"
     CDS             7389..8207
                     /gene="amrB"
                     /locus_tag="VKN64_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HMA59011.1"
                     /translation="MSLLMAGFSPHPPLAIPDIGRDNLKKVSDTIDSLKELGREVVES
                     DPELLITISPHGTVMRESISIIDEDVVRGDFGQFGYSNISFERKVDTHFIDILKKNAS
                     KEGFHTISLKDRDGTKLDHGVLVPLYYIQEAGLKDIPIVPINMAFWDYHELYEFGKII
                     NQTIEEENIDAVAVASGDLSHRLKPGAPAGYDENAEEFDKEFVRLIDENQLAQIKDME
                     KGLIHRAGECGHRPMVILSGILSEHKVQTDLKSYEGPFGVGYAVASFKILAGGN"
     gene            8211..8723
                     /gene="amrA"
                     /locus_tag="VKN64_01880"
     CDS             8211..8723
                     /gene="amrA"
                     /locus_tag="VKN64_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HMA59012.1"
                     /translation="MEEEMGYVELARKTIENYIKEKRKIDPPQSGKFQKRAGAFVSIK
                     KSGQLRGCIGTIEPTRPFLGQEIVENAISASTRDPRFQPITEDELNQIDISVDILGEK
                     EPVEDISQLDPEEYGVIVKKNSRTGLLLPNLEGVDTVERQLEIARKKAGISAQEDYQV
                     LRFKVERYEE"
     gene            8713..9705
                     /gene="amrS"
                     /locus_tag="VKN64_01885"
     CDS             8713..9705
                     /gene="amrS"
                     /locus_tag="VKN64_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HMA59013.1"
                     /translation="MKNKKKEAMFYEKKEGETVQCQLCPHNCVINEGKRGICRVRINE
                     EGTLYTENYGEISSMGVDPIEKKPLYHFYPSQGILSLGTYGCNFKCKFCQNFRISQQQ
                     PTTTYQTPSDIIKQAKDRNIMGIAYTYSEPMVWYEFVYETAIKAHEAGLNNVLVTNGY
                     LNQKPLKKLTPYIDAANIDLKSYHNDFYKEICGGTLQPVLETIKYMYGKTHLELTTLI
                     VTNHNDGLDELEDIFKWVSDLNEDIPMHLSRYFPNYKMDEPATPERKMEAAYQLAKKY
                     LNYVYIGNMRSKKGQNTYCPDCGTEVITRHYFNAQNKLEEGKCPECGKQILEYY"
     gene            complement(9702..10424)
                     /locus_tag="VKN64_01890"
     CDS             complement(9702..10424)
                     /locus_tag="VKN64_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59014.1"
                     /translation="MNSKTNWINRINYKDIIIIYTLWFFTLIFNRFFDFASFMQVPAL
                     SGFFDLLFHFAARLIFISLIFLYFVFIYDLSFKELGISFKSLTKKLISLIIYLIILLG
                     LVILLINFPLSTDNISRIFKPLYKVLTMETFVRSTLPLILVFPIFFIIAFSEQLMLNL
                     FVYEVFNYKLPKLVSKLMSALFFSFLIYHLSPDKIIIYFIFALISVTLYERAEKSILV
                     PSFFGAGFYLIYTFYIYGWNFI"
     gene            10545..11312
                     /gene="cdaA"
                     /locus_tag="VKN64_01895"
     CDS             10545..11312
                     /gene="cdaA"
                     /locus_tag="VKN64_01895"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HMA59015.1"
                     /translation="MIWSVITNIIDLFIVGAIIYFLLNLIKGTRAVQLIKGLVLIFIA
                     SVVSQLLNLNTVHWFLQSVLTMLIVALPIVFHPELRRALEQLGRGGFLSNQLVHKWSA
                     KDINEIIMASDELAEEKTGALIVFENEVGLRDYIETGTEINAQISKELIENLFYKSAP
                     LHDGAVIVGEGRVKAAGCLLNLSNKPSINPKLGTRHRAAIGITEESDAIVIVVSEESG
                     VISLAQDGELIRYLDKSKLREILFKEFVPRKTNDFFS"
     gene            11328..11744
                     /locus_tag="VKN64_01900"
     CDS             11328..11744
                     /locus_tag="VKN64_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59016.1"
                     /translation="MREFYNKYKIIIIAVMIAFVLWLYVNYQEGNLQGSFEFGSNQIR
                     LNIQYENLTDKLVVLDISDQTVLIEPEGYRFFNTYGPEDFEAYIDLREVQIGDNIRKV
                     NIRTPQGVQINTQEPSIVRVTIASYREEGSQQEAQN"
     gene            11790..13136
                     /gene="glmM"
                     /locus_tag="VKN64_01905"
     CDS             11790..13136
                     /gene="glmM"
                     /locus_tag="VKN64_01905"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMA59017.1"
                     /translation="MGKIFGTDGVRGIANREITPELALKLGKAGGLFLKEKSKMNGNF
                     FVLIGKDTRISGSMLESALIAGFNSVGVNIVKLGIISTPAVSFLTDKTDAIGGVMISA
                     SHNPIEDNGIKFFSEKGFKLTDQEELQIEEYIFNEKEITKPIGTEVGDIIKREKLIHQ
                     YVDYLEDTVDSNFNDLKIVLDCANGAAYEIAPQIFNKLGAEVIAVNDKPDGRQINLDC
                     GSTKPETILEETKKYKADLGIAYDGDADRLIMVDEKGNLIDGDHIMAFLALNMLKEDK
                     LDKKTLVTTRYSNLGLEELLNERGARVVKCKNGDRYVLQTLLENGYNLGGEKSGHIIM
                     LDYNKTGDGVLTSLKVVDYLQKYGQPLSQINDIFKPWPQELINVRVKDKEKLSTNKRI
                     NELIKEIEDDYSKQGRIFVRASGTEPVIRVMLEAKDEAMISKWEDKIASTIKEELN"
     gene            13367..15193
                     /gene="glmS"
                     /locus_tag="VKN64_01910"
     CDS             13367..15193
                     /gene="glmS"
                     /locus_tag="VKN64_01910"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMA59018.1"
                     /translation="MCGIVGYVGEKKAASILYDGLKRLEYRGYDSSGIAVNDGLNIDV
                     VKKKGKLEQLKKNIKGNVNGGTTGIGHTRWATHGQPSDINSHPHSDCKDNFVIAHNGI
                     IENFQNIKKYLKKEGHIFKSETDTEVIAHLLEDYYEGDILKALKKTIERLEGSYALTI
                     LSKHDPDKIYVARKDSPLIIGIGEDENYIASDIPAFLGETRDFYILEDGEFAVINKDE
                     IDIFDFNLAPKEKEVYHVDWDPEMAEKQGYDHFMLKEIHEQPDAVRRLMANRVTPEGI
                     NLAELNLDKEFLNNLNQVHLIACGTAYHSCLIGKYMMEDLSELSVEVDVASEYRYRNV
                     PIKENDLVIAVSQSGETSDTLASLRLAKEKGAKVLAFTNSRGSSIAREADEVMLLKAG
                     PEISVASTKAYLNMVVAFYMFSLFLADKQNKIANNRYLEMSKDLIKLPDLLDDVIEKS
                     ERVMKDRADQYANSENAFFIGRHTDYTLAMEGALKLKEISYIHAEAYPAGELKHGTLA
                     LIEEGVPVVALGTRETVFEKTLSNITEVKARGGDVTAIVHKDAKDIEDVVDHIVYIPE
                     VNELFTGALAIVPLQLLAYYTALLRGCDIDQPRNLAKSVTVE"
     gene            15288..15701
                     /gene="acpS"
                     /locus_tag="VKN64_01915"
     CDS             15288..15701
                     /gene="acpS"
                     /locus_tag="VKN64_01915"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HMA59019.1"
                     /translation="MTANKNGKPKKNEVIALGVDILEVDRIKEIIEKRGDKFLKRVFT
                     PKEIKYCQSQKRSIESFAVRFSAKEAFIKAIESEINIPYQDIEVDKKANKKPKINLHG
                     KALLAAEEKNVAEILVSLSHEKNYVTANIILKGRI"
     gene            15701..17209
                     /locus_tag="VKN64_01920"
     CDS             15701..17209
                     /locus_tag="VKN64_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMA59020.1"
                     /translation="MIVLTPEEMAKLDQKVIDNGYESLILMETAGRGTFQSLQEKYDR
                     DNKILILAGSGNNGGDGLVIGRLLDICKYNVKIIIVGNQNKLNKDPLTNYKICELRDM
                     DIQFFEQDSDLQLIKNAITESDVIVDSLLGTGLKGDLRSPYLEIVKMINDSKNKVLAV
                     DIPSGINGRNGQVMKEAVKADYTVTMAFSKIGHHLYPGRAYTGELQRVDLGFPEHLIE
                     YSKYKHHSLTLQEAGNLLPKRAKTGHKGSFGKVLVIGGSKGLEGAPALSGKSALKMGT
                     GLVKTMIPSEISSTVSSFSPELISDDLTIENIEKDSKDYDVIALGPGLGIGEFQKEIV
                     SYLINKAELPIVIDADGLNNLNLKELKNAKSNLVLTPHPGEFARLIGKSIPEVQANRL
                     KYVREFVDKYKVNLVLKGASSLVSDPAGNIYINPTGNEGMATAGSGDVLTGIISSLIG
                     QGLNTFNSAVLGVYLHGLAGDISTDKLGSHSVVAQNLIENIPPAISKIKRGC"
     gene            17211..17666
                     /locus_tag="VKN64_01925"
     CDS             17211..17666
                     /locus_tag="VKN64_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA59021.1"
                     /translation="MITAKDIMTTDVVTVDPKTSVKEAANIMSEKGISGLPVVQNGEL
                     VGIVTENDLIIKDKKLHFPEYINVIGGIIYLESYKKFREEFKKFTAVNVEELMTKDVI
                     TVSPDDSIEDIATIMHQKNVNRLPVVDNSGQLIGIVTRANIVKHIAVEE"
     gene            17688..18230
                     /gene="dcd"
                     /locus_tag="VKN64_01930"
     CDS             17688..18230
                     /gene="dcd"
                     /locus_tag="VKN64_01930"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMA59022.1"
                     /translation="MIISDKTIKNLIEEETLVIEPFEDYLIQPASIDLRLGTNYLKID
                     ENQMEVMTLEDKLNYNQIDSDEIVIPPNSFLLATTEEYIQLPNNLTAFVEGRSSIGRM
                     GLFIQNAGWVDPGFSGKITLELFNANRLPIRLKAGRRICQLVIAEMDQEAEFPYQGKY
                     QGQDTAVGSKVFEDNENFKD"
     gene            18289..20538
                     /locus_tag="VKN64_01935"
     CDS             18289..20538
                     /locus_tag="VKN64_01935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IGHMBP2 family helicase"
                     /protein_id="HMA59023.1"
                     /translation="MQNIIIRDIDKHIGPGDIVGAFINEANINSNDIGNINIRDNMAE
                     VEVKNEVCDRVVNIMDNNKIGGVKVHLEVDDDAERRQKVTRYYNKFKQLVELERQEEM
                     ERHEIEIKRLSSRAREKKGRALLKMRGKGDGQTYDHKPKVKFMKEEVGEKLPDTEISI
                     GDLVMVSKNRPLDDNNPTGTVAEKTNYSMTVVFDENPPGFVFGDSLRVDLYVNDITFQ
                     RMLDALKNFRNAKSETRLGELQRKMLGLEEINWKDESFDEIEWVNTELNQSQKEAVKS
                     SLKAKDFSLIQGPPGTGKTMTAIEIINQGVKEEKEILATADSNVAVDNLVERLVNMGT
                     EVLRLGHPIRVTPLLRKHTLDYKILDHPDYKEAERLREKAQDLLDQQDQYQHPGNSLR
                     RGMSDKMIKEKAEQEQGARGVKPAIIKDMAKWLEIQDEIDGLFNRIEKLEQKAIEDLL
                     ETAEVICTTNSTAGSELLGEYDFDLIVIDEATQSTEPASLIPFIKGDKVVLIGDHKQL
                     PPTILSQKASEQGLSISMFERLYEIYGDEIRSMLEIQYRMHDDIMDFSNHEFYNGKLK
                     SSPEVAEHTLRDLGYNMEGKKCFTDKSLNPDQPIVFIDTVDMTAEERSLKGSNSYDNP
                     VEMEILLDIVDEALKSALKPEDIAVIAPYKDQVDLLKKHTNIENLEINTVDGFQGREK
                     EIVLISLVRSNESNNIGFLRDLRRLNVALTRAKRKLVVIGDSSTIAKNETYQNLLDYI
                     EERGLYYTL"
     gene            20669..20947
                     /locus_tag="VKN64_01940"
     CDS             20669..20947
                     /locus_tag="VKN64_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HMA59024.1"
                     /translation="MEEEKKEITIEISKGLLKDLDDYIKYRRVSRDKFISDAINHYLC
                     EHKKKELREQLKKGYIEMSELNLELAHEGLKCDSNSIKIYEKCLVECE"
     gene            20952..21302
                     /locus_tag="VKN64_01945"
     CDS             20952..21302
                     /locus_tag="VKN64_01945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMA59025.1"
                     /translation="MDLKRGDVYYADLNPVVGSEQGGVRPVLVIQNDIGNKYSPTIIV
                     AAITSKINKAQLPTHVEISADDSSLDRDSVILLEQIRTIDKKRLKSEVAKLNGDILER
                     VNSAIEISLGLVNL"
     gene            21338..22351
                     /locus_tag="VKN64_01950"
     CDS             21338..22351
                     /locus_tag="VKN64_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HMA59026.1"
                     /translation="MSEYIADIYRSNLVESRHRGDVAVVDPYGKLKYFSGNPDNQTYL
                     RSAAKPFQILPVINSGAADFFNFTKQEIAVMSASHNGEEKHVKTVREILEKIEVSEEK
                     LNCGIHDPYFKKAAHKLYLDGNKPTDIHNNCSGKHAALLALCKFNDWDLDNYLAKEHP
                     VQQLMLTTISEVSDIERTDIYLGEDGCGVVVFGLPVKKMAYAFARLANPDYLPNKYKE
                     AARRITKSINDHPDMIAGTKRFNTDLIEVLGDKLTGKMGAEGVFCFGLYNGPGVSVKV
                     EDGNSRALPPVVIDILKTLDYLTEEELSQLQRYVRPVIKNHHDHEVGYIKSILNLKKD
                     VNR"
     gene            22353..23519
                     /locus_tag="VKN64_01955"
     CDS             22353..23519
                     /locus_tag="VKN64_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA59027.1"
                     /translation="MILIKDAQILTMADNDYKQGSILIKDGKIAKISENIEIKDEYEV
                     IDAKDQIVMPGVIDAHTHLGIGEEGVGWEGQDYNEMTDPFTPQLRAIDAVNPDDYGIK
                     EALEHGVTTIMTGPGSANVVGGESMVIKTTGKTIDEMVLKNPAGIKAAFGENPKRVYN
                     EQKKLPTTRMGIAAVMRENLMKAEDYLAKKEKAQKDDEPFDRDIKMESLIRIIKKELP
                     LKVHAHRADDIMTAIRIAKEFDIDITLEHCTEGHKVADEIAEANIPAIVGPSMTGRVK
                     VELSELSFKTAGVLAKAGVKIALMSDHPVIPTKNATIYAALAIKGGLEEKEALKAITI
                     NPAEILGVDDRVGSIEEGKDADLIIFKGHPLDISSDIQRVIVNGEVVKREEDEK"
     gene            23525..23887
                     /locus_tag="VKN64_01960"
     CDS             23525..23887
                     /locus_tag="VKN64_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59028.1"
                     /translation="MKLKDIIDKLELEGVHENKELLELDIKKACGADLMSDVLAFSDA
                     KTMLITGLTNPQVIRTAEMIGIKVIIFVRGKSPVEDTVKLAKENNISLYQTEKPMFQT
                     CGILYNLGLKPEKMVKIE"
     gene            23902..24330
                     /locus_tag="VKN64_01965"
     CDS             23902..24330
                     /locus_tag="VKN64_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA59029.1"
                     /translation="MKRKKIIDRQIERRDFASGGEVSSKLKKLLRKLGVNNKDIRRTS
                     IITYELEMNLIIHSEGGRLKAFVTPKSITIFSEDKGPGIENVDKAFEPGYSTASDEIR
                     EMGFGAGMGLNNVKRFSDDLEVESEVGEYTKLKATINLNQ"
     gene            24347..25672
                     /locus_tag="VKN64_01970"
     CDS             24347..25672
                     /locus_tag="VKN64_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HMA59030.1"
                     /translation="MSDKHSVLLKEDKCEGCTNCVKNCPTKAIRVHQGKATIKEDLCI
                     DCAECIRTCEYHAKYAETNDLAELATNEYNIALLAPSFYGQFDSSFDPEKIKKAVYKL
                     GFDEVWDVAYAAEAISQKTYQFLQNNDGTYISASCPVIVRLISLLYPELLDNIVPFNS
                     PVDLMADYVKNKVEAEGREQYGMYFITPCPAKVTAIKNPLGRSESSLTGAIAVDKIYP
                     KLLELIKGDNNFKEVELKQKYQPYMGISWGQSGGENDILNEEGIIDTLSVSGIHNIKK
                     VLEELTRGNIKGIKYLELVACPTGCVGGIFNVNNPFQAKYNIRMNIRNHKEFIDQDLN
                     EYDFKLEEKIKEIGAEKLDEDFETAIGKLEELEKEIERLPGLDCAACGAPDCETFAED
                     VVNGLAKRSDCIFVLRKEIGDLADQLSKLTHELPPVMNSEENQKKDKEE"
     gene            25688..26017
                     /locus_tag="VKN64_01975"
     CDS             25688..26017
                     /locus_tag="VKN64_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HMA59031.1"
                     /translation="MQLKEITESLHLEVLNEADLTKEVKGCYIGDLLSNVMARAREGD
                     IWVTIQGHQNIVAVALLADAAAVVVAEEAEVDEKSIKRADEKDVNLLRSKLSAYELAQ
                     EFTKMGV"
     gene            26019..26753
                     /locus_tag="VKN64_01980"
     CDS             26019..26753
                     /locus_tag="VKN64_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA59032.1"
                     /translation="MKKYRLDLHIHSVLSPCADILMTPGNIIKEAEKKGINIISITDH
                     NVFGNVESAIKIAEEKDITVLPGIEVQSREEIHLLSYFNSLKDLKEYGKIIYEGLEGI
                     KNDEEKFGAQILVDKNDEFVGKEEKLLLNSTKYSVEELVKITYQNNGIPVPSHAERSF
                     GIIQNLGFIPGDLEISFVEMNFHKTYEDYLKEFPYLKKYKLLKNSDSHYLNQIKPTME
                     IYLDEKPNSEKFFSYLKSHKIEEYLL"
     gene            26870..27358
                     /gene="nuoE"
                     /locus_tag="VKN64_01985"
     CDS             26870..27358
                     /gene="nuoE"
                     /locus_tag="VKN64_01985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMA59033.1"
                     /translation="MSCRCDQKNMDKYLAPLNEILKEYTKEEKYLIPILQDAQDEYGY
                     LPEAVLKEIACNLDLSLSQVFGVVTFYSQFHLEPRGENIIRVCTGTACHVRGGAEVLD
                     QLKEELNIGSGETTDDLTFTLETVACIGACGLAPVIMVNDDTYGRMKPGKVSEVLANY
                     QE"
     gene            27358..27909
                     /locus_tag="VKN64_01990"
     CDS             27358..27909
                     /locus_tag="VKN64_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA59034.1"
                     /translation="MKELALHILDIAENSIKAETDELNIEINEDTRENEFEIVIEDDG
                     KGIEKEKLENITDPFVTSRKTRPVGLGLSLFKAAAQRCNGNFEIDSDSNGTSVKAVFE
                     HDHIDRAPLGNMAQSIVSILLWDSNLELTYEHRYNENQFIFTTKEIKKELEDVKITDN
                     KILNWLKDYIDENIREIRGGEFN"
     gene            27906..28271
                     /locus_tag="VKN64_01995"
     CDS             27906..28271
                     /locus_tag="VKN64_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HMA59035.1"
                     /translation="MKSLEELRELREKVEKDMQMRKETNKPKIIIGMGTCGIAAGARE
                     ILKAVMEEIDKRDLDVEVTQTGCIGMCEKEPLMDVKLPGKDRITYGNLTREDVKRIVV
                     NHVINGNVVDELVIARLAE"
     gene            28292..30094
                     /gene="nuoF"
                     /locus_tag="VKN64_02000"
     CDS             28292..30094
                     /gene="nuoF"
                     /locus_tag="VKN64_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMA59036.1"
                     /translation="MKDTIYRSHVLICTGTGCVSSGSKKIKHELQDQLADKDLLDEIK
                     IVETGCHGFCEKGPIMVVYPEGVFYCQLKEEDVAEIVDEHLLKGRIVERLLFKEPMTE
                     ESIPSYQDIDFYKKQHRIALANCGKINPESIEEYIANNGYEAAGQALTSMTPQKIIEE
                     VKNSGLRGRGGGGFPTGLKWQFARDNESDKKYVICNADEGDPGAFMDRSILEGDPHRI
                     IEGMIIAGYAIGADEGYVYVRAEYPLAIERLQKAINDAEELGLLGEDIFGSGFNFKLN
                     IKKGAGAFVCGEETALMASIEGKRGMPNPRPPYPAEKGLWGKPTNINNVETYANIPYI
                     ISEGSEVFSSIGTENSKGTKVFALTGKIKNTGLVEVPMGTTLKEVVFDIGGGLTEDKK
                     FKAIQTGGPSGGCIPEDYLDLPIDYDSLIDVGAMMGSGGMVVMDESTCMVDVARFFLS
                     FTQNESCGKCTPCREGTKRMLEILNRICEGEGKEGDIKLLKELGEHISETALCGLGQT
                     APNPVLSTIKYFRNEYEAHIFDNKCPAGVCQELASAYTINKETCVGCGQCVKVCPVDA
                     ITKGDGNYYVIDDEICISCGACEKKCPVDAISQG"
     gene            30123..31844
                     /locus_tag="VKN64_02005"
     CDS             30123..31844
                     /locus_tag="VKN64_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HMA59037.1"
                     /translation="MGKVSLTIDEQNIEVEKGTTILEAAKEVGIDVPYLCYHPDLTLH
                     GACRVCVVEDLEDNSLVASCVTPVTEGMEISTTSMKARRARRRNVNLLLANHPNDCLG
                     CDRNGSCELQDITHSLGITREDVEEIAGEVREVERDEVGPALKRDPNKCILCGRCVRV
                     CEEVQGVSALQFSNRGFDSIVTTAFDLPQSEINCANCGQCATVCPVGAITEVSEIQKV
                     WHALEDEDKHVIAQTAPSIQATIGEEFGYEPGTVVTGQLVSALRKLGFDKVFSTEFTA
                     DLTILEEGSEFLERLTANKNLPHITSCCPGWVKYAEHNYSDLLNHLSSAKSPQQMFSA
                     LSKTYYAEKADIDPEKIYTVSIMPCTAKKFEKDRDEINATEYKDTDAVLTTRELARMI
                     KEVGLQFKNLPNEEYDELMGEHTGAGTIFGTTGGVAEAAVRTVKEKLTGEELERLNLG
                     FRGINKAEVVINDQIIKVAIANGLRNAEKLLDEVRAGESEYHFIEVMACPYGCVGGGG
                     QPLPISEEHKELRGKGLANADEKNVVRKSHENPMIKKLYEEYLEEPNGEKSHHLLHTT
                     YHERPKN"
     gene            31985..32470
                     /gene="tsaE"
                     /locus_tag="VKN64_02010"
     CDS             31985..32470
                     /gene="tsaE"
                     /locus_tag="VKN64_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMA59038.1"
                     /translation="MKYITNSPEETKMIGQRISEIIKEDKNYFSQIILLTGDLGAGKT
                     VLVKGLAEGLDINFNITSPTFNLINEYEGEPGLIHMDFYRIDKKEELLQLGLEEYMAM
                     DNVKAIEWPQLIFDYLQAEFIFIKIKNMDGDKRKIIIEAEGPKSKNILKGLKDNVSIG
                     N"
     gene            32451..33170
                     /gene="tsaB"
                     /locus_tag="VKN64_02015"
     CDS             32451..33170
                     /gene="tsaB"
                     /locus_tag="VKN64_02015"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMA59039.1"
                     /translation="MLVLGIDTSTDICSVGLSNKEDFIGEINIKLKRRHSERLLPIIK
                     RIFSETGYEIQDLDGLAVTDGPGSFTGLRIGLSTVQAFKSALNIPVYSVSSLEYMAYS
                     VSQHYENYLLVPTIDARNKRVYTSVFTSEPQQKLLKRIRDDKAVEVKSLIEDLRKYKK
                     GKVIFGSGTDSYYEIFKKANLDNTKIIYKIRNSGGYNLACLGYKYLSAGQDTDYSELV
                     PKYLKKPQARLNWEKKYLQED"
     gene            33172..33624
                     /gene="rimI"
                     /locus_tag="VKN64_02020"
     CDS             33172..33624
                     /gene="rimI"
                     /locus_tag="VKN64_02020"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HMA59040.1"
                     /translation="MDLCLDVMKLDDLGEVIKIEREAFSNPWNMKFFKEELIHNSFAL
                     YLTAKNDNELVGYVGCWFKDHANEVHIVNLAVKKTKRRQGIGSYLIKEIVDMSKNLNA
                     DTLTLEVRITNKAAIDLYIQLGFTKAGLTPNYYLDNDEDALLMKKELN"
     gene            33626..34639
                     /gene="tsaD"
                     /locus_tag="VKN64_02025"
     CDS             33626..34639
                     /gene="tsaD"
                     /locus_tag="VKN64_02025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMA59041.1"
                     /translation="MNEDIKILAVETSCDETAAAVSKNGLDILASVVASQVDWHKKYG
                     GVVPEIASRKHMEFINPVVEEALEKADIKLAELDAVAATYGPGLVGSLLVGLSYAKAL
                     ALALNKPFIGVNHIAGHIYANFISKKDIKTPVICLTVSGGHTDLLYFEEYGDYEILGR
                     TRDDAAGEAFDKIARYLGLGYPGGPIIEKRAKLGKEDAVKFPRPFIDKDTYDFSFSGL
                     KTAVINYIHNQKQKGNELDINDIAASFQRAVIDILTAKTVRAALNKKVNSVILSGGVA
                     ANKRFREVLRERLKKEGIPLYYPDLELCTDNAAMISTVAYYQYKKGEFADFALNADPN
                     LQL"
     gene            34649..>35383
                     /locus_tag="VKN64_02030"
     CDS             34649..>35383
                     /locus_tag="VKN64_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA59042.1"
                     /translation="MEKNKREYYLYKSVQDDNILPVTSKCNLSCIFCSHQQNPKELEI
                     ISFGDLKLKKINDLIDYINPNQPIIMGESATKIIEGEPLLHPDFKEIIKRIRTKYNDT
                     EIRITTNGALLDKNLINFFTKIKKITLNISVNYLEPELREKVMGDNCNQNINTTLQKL
                     KYSNIEYNFSMVALPHLYGYQRIKEEIIKMAEYEPLTIRVFMPGYTSLTAADLKFDHK
                     EVYQKLSKMVDEINTFQKTPVTIEPPM"
BASE COUNT        12736 a         5328 c         7629 g         9690 t
ORIGIN      
        1 ttcaccttat gatttaagtc gtggtagaat tacttaccgt cataaagcaa aataatttgc
       61 ttaaggaggg atgttaggtg aaggtaagac catctgttaa acctatatgt gataaatgta
      121 aagtaataag acgtaatggt aaagttatgg ttatttgtga aaatcctaag cataaacagc
      181 gtcaaggtta aaatattaag gttggaggtg tataatattg gcacgtattg aaggtgttga
      241 tttaccaaga aataaaagag tggagattgg cctaacctat atttacggta ttggtcgttc
      301 tacatctcaa aatattttag ataacactgg tgttaatcca gatacaagag taaaagatct
      361 aactgaggcg gagattagta aaatcagaaa agaaattgat aaatacccgg ttgagggtga
      421 actacgccgt gaagttagag caaatattaa aagattaaaa gacataggct gttaccgcgg
      481 tctaagacat cgtagaggcc taccagttag aggtcagaga acaagaacta atgcaagaac
      541 ccgtaaagga cctaagaaga ctgtaggagc aaaacgtagc aaagctacta aaactgcaaa
      601 ataattaagt ggttaggagg tatatagatg gctaaatcca agaaaaaagg caagaaaaga
      661 ggccgtgcta agaaaaagaa gatcaatgtt gatagcggta ttgtgcatat caaatcaaca
      721 tttaacaata caataatcac agtaactgat aaagaaggaa atgttattct ttggcacagt
      781 gctggtaaag ctggctatca gggttcaaga aaaagcacac cttttgcagc ccaaattgca
      841 gctgaagaag ctgcccagga agcaaaagat ttaggattaa agtcagttga aatatatgta
      901 aaaggaccgg gctctggcag agaatctgca attagatcta tccaggccac cggtttagat
      961 gttagtttaa ttaaggatat cacaccaatt ccacataatg gatgtcgtcc acctaagaga
     1021 cgtcgtgtgt aattataata ataaattgac aacaagaata tggaggtgta ataatgtcaa
     1081 gatatacagg ttcagtatgt aagttatgcc gtactgaagg tgaaaaacta tatctaaaag
     1141 gagcacgctg ctattctgat aagtgtgctt atgagagaaa acctaattct cccggtgagc
     1201 aaggtactgg tagaagaagg agaaaagtat ctgaatatgg ttcacagtta agagaaaaac
     1261 aaaaagttag aaggatttat ggattacagg aaaataaatt ccatagatat ttcaaggaag
     1321 cagaaagaga taatggaata accggtgaag tcttcttaca actattagaa agaagactag
     1381 acagcgctgt ttatagaatg ggttttgccc gttccagaaa tgaggcaaga cagttcgtct
     1441 tacacggaca tattgaagta aacggtgaaa aagttaatat tccatcatat aaagtagagg
     1501 aagatgatgt aatttctgtg aaagaatcta gcttcaaaaa ggatactttc caggaaatat
     1561 tcgatgtaaa tgctaactta accccacccg agtgggtctc tgtagattta gaaaaagggg
     1621 aaggtactgt agtatccctg cctacaagag aagatattga atatccagtg gaagaacact
     1681 taatcgttga gttttattct agataataaa gccagtcctc gaatctgtta aagtcaagaa
     1741 ataaggaggt tttaaattta atgatagaaa tagaaaaacc tcaaatagag aggctagaaa
     1801 gttcagataa atacggcgaa ttcgaaatct ctcctctcga gagaggttat ggaataaccc
     1861 tcggaaactc tttgcgcagg atactactat cttctttacc cggtgcagca attacctctg
     1921 ttaaaataga aggggtacgc catgaattct cttctattga aggagtaaaa gaagatgtaa
     1981 ccgaaattat tcttaattta aaagaagtag ttttaaggta taaaggcgaa gaaatgcaga
     2041 ctatgaattt aaatgtagaa ggtaaaggag aagttactgc tggtgatttc agaacttctg
     2101 gcgaagtaga agtcattaat aaagatcatc atatcgctac tctagcagaa ggcggtaaaa
     2161 taatgatgga agtaactgta gaaagtggaa gaggttatgt cctagcagaa gaaaacaaag
     2221 aacactttga taaagtaatt ggtctaatac caattgattc ttcctttagc ccgattgaaa
     2281 gagctaattt cagagtagaa aatacaagag ttggccaggt aacagactat gaccgtcttg
     2341 taatcgaggt tgaaaccaat ggaagtatta cacctgatga cgcaatcagt ttaggggcta
     2401 aaataatggt tgaacatctt gaactattca tcaatctcac cgatgaaatt gaagatgttg
     2461 agatcatggt agaacaggaa gaagaagaac aaaacaaagt attagagaca acaattgaag
     2521 agcttgaact ttctgttcgt tcttcaaact gtctaaaaag agccggcatt aacaatgtag
     2581 gcgaattgat tgaaaagacg gaagatgatt tgatgaacgt aagaaactta ggaaagaaat
     2641 ccttacagga aatcaaagat aaattagatg aattagatct cagcttaaaa gaaccagagg
     2701 tttaaaggag ggagattgaa atggctaagc gtaagttaaa caagaaaagt tctcatagaa
     2761 aagctttatt taaaaatcaa ttaatcagct tatttaaaaa tgagcaaatt aaaactaccc
     2821 tgccgaaagc caaagaatta agaccaattg cagaaaaaat gatcactaaa gcaaagaaaa
     2881 acaatgttaa tgcaagaaga caggcgatgg gtaaattaaa tgataaagaa gcagtcgaaa
     2941 aactgtttga tgtgatagct ccccgttata tggaaagacc gggcggttat accaaaatta
     3001 ttaaacttta tcccagacgc ggagacgcat ctgagatggc tttaatcaag ctagtggaag
     3061 aagagtaaag tttatttagg caaatttagt gggtgtattt tatgagttta ctggaagtac
     3121 aaaatgtgga atttaaatat cacggtcaga cagaaccagc tttaaaagat ataaattttg
     3181 aggttgaaca aggagacttt gtttccgtca ttggagcaaa tggatctggt aagtcaactc
     3241 ttgccaaact ccttaacgtt cttttgagac cggataaagg aaatataatt gtagaaggac
     3301 aaaatactcg taataaagaa aagatctggg atataaggaa aaatgtcggt atggtctttc
     3361 aaaatccaga taaccaaata gttgctacca tggtagaaga tgatgtagca tttggtctgg
     3421 aaaatatagc aataccctcc agtgaaataa gaaaaagagt agaccatgct ttggatttgg
     3481 ttgatctaac gggttatgaa acccatgaga cccataaact ttccggcggc caaaaacaga
     3541 ggttagcgat tgccggaatt attgctatgg aaccaagctg tatagtctta gatgaaccga
     3601 cagcaatgtt agatcctcag ggcagaaaag aagtaattga taccattaaa ttcttaaaca
     3661 aagaaaagaa tataacaatt gtcctaatta cacattttat ggaagaagcc agtatctctg
     3721 ataaggttta tgtgatgaat caaggtcaga ttcaatcaca gggtccacct gctgaagtat
     3781 ttaaaaaaat agattactta agaagcataa atttagatgt tcctgtagtc atggaagttg
     3841 ctaatggttt aaagaataag ggtgttaata tacccgatat cttaacaatt gatgggttgg
     3901 tggatgcttt atgctaatag aattacaaaa tgtcacccat atttatgacc aggaacagtc
     3961 tatcaaagct ttagatgata tcgatctaaa gatagatgaa catgaattta ttgggatcgt
     4021 aggtcatact ggctcaggta aaaccacctt agttcagatg tttaatagtt taatcaagcc
     4081 tacatccggt tcaatttatg ttgatggaat caataccact gagaagaagg ctgatttaaa
     4141 gcatgttcga cagacagtgg gtttggtatt tcaatatcca gaacatcaac tatttgaaga
     4201 aactatttat gatgatgttg cctttggacc cagaaacctc aaactgggaa aagaagaagt
     4261 tgataaaagg gttaaggtcg ctttagattt ggttggttta gattttgaag agtttaaaga
     4321 ccgttcacct tttaatctct ctggtggtca acaacgtagg gttgcaattg ccggggttct
     4381 ggccatgcgg ccgcgagtat taatacttga tgaaccctct gctggtttag acccccaagg
     4441 gagagaaaag ctaaaggctt tacttaaaag gctgcataaa tcatataata ttacaataat
     4501 attaatatca catagaatgg aagaaatcac acagttatca agtagggtaa ttgttatgga
     4561 tcacggtaag atagtgctaa acggaccccc cagagaggta tttagccata aggagaagtt
     4621 ggaaaaacta tctttagaat taccggaaat taccgagata ctctttcgtt taagagaaag
     4681 aggttgtgat attagaaccg acatttttac agtggaagag gctgtagatg aaattattaa
     4741 ggtaattggg agtcaagaaa atgttaactg atatcacaat agggcagtat gtacatggta
     4801 attcaattgt acatatcctg gatccgagga taaaaataat aaatacaatt attttaatta
     4861 ccagcttatt tttaatagca aacttctggg gatttgcgat atttttagga ttcaacttgt
     4921 ttgttatata tatttcaaat cttccgttaa caaaggtgct gaaaggctta aaaccgatct
     4981 tatttttggt cttacttacg gtcatcttac acatattcat gacccgtggg ggagaagttc
     5041 tctttcagtg gaagttttta aggattgaaa gtgaaggtct atatactggt acttttatgg
     5101 ccagccgcat tcttttatta ataatgttta cttcgctttt aactttaaca acatcaccct
     5161 tacagctcac tgacggtatc gaatatatct taaaaccgtt aaaaagaata ggagtgccgg
     5221 ctagtgagat ctcgatgatg atgaccattg ccttaaggtt tatacctaca ttaatggaag
     5281 aggccgaaaa aattatgaaa gcccaaaaag ctagaggtgc agactttgaa agtggtaatt
     5341 taattcagag ggctaaaaat ttaattcctc ttttggtacc gcttttcatt agtgctttta
     5401 gaagggccga cgaactagct ttagctatgg aatcaagatg ttatcagggc ggtaaaaaaa
     5461 ggaccagact gcatatcatg cagatcaagc ggagagatgt aatagcactg attatttcga
     5521 tagcattagc agtagttgta agtttttatt aggagtttag atatgcgttt ttataaagta
     5581 attctcgaat atgacggcac taattataat ggttggcagc gtcaaaaaaa taccgatact
     5641 acaatccagc agattgtaga agagaagtta agtataatca ataaaagttt tgtactggct
     5701 acttcagccg gccgaaccga tgccggtgtg catgccaaaa gacaggttgt caacttcaaa
     5761 ttagatgtcg atgtacctgt tagaaggata ccatttgctt taaatagttt attaccaaaa
     5821 gatatcattt gtacagattc agaagaggta gatgaagatt ttcatgcccg gcatgatgct
     5881 aaaggtaaat tataccgata caggttaagt aactctcggt ttccttcggt ttttacgcgc
     5941 aattatgtat ataatattag gagtaagatt gattttaaaa agataaaaaa agcagccccg
     6001 ttactggttg gtacccacga ttttgcttcc tttcaaaata gtgggagtga tatggaagac
     6061 acggtaagga ctattgaaaa aattgatatt tatcaagttg aagatgaata tcattttgat
     6121 ataaaaggca gcggattctt atataacatg gtccgcatta taatgggaac ctttattgaa
     6181 attggacagg ataaaattaa attagatgac ttggaagata ttatggagtc taaaaatcgg
     6241 agaagagccg gttttacagc tccagctaaa ggtttgactt tgatggatgt ttattactag
     6301 ttgcttgaca agggttttaa attataatag aattaatgtg gataattttg tgaaatttaa
     6361 taaatattaa tatagataat aaggggggaa aaagatgaca acatatatgg caaaacctga
     6421 tgaagttgat aaaaattggt atgtcgttga tgctgaagat aaaactttag gtagattagc
     6481 ctccagaatt gccacagtat taagaggaaa gcacaaacca gaatttactc ctcatgttga
     6541 cacaggagat tttgtaatcg ttgttaatgc tggtaaagta aagcttaccg gtaataagtg
     6601 ggatcaaaag gttcactata agcacagtag atatcccggt ggtttgaaaa ccatgactta
     6661 tggtgatctt aagaaacaca atcctaaaga aattgtaaga catgctgtag atggtatggt
     6721 accaaataat aaattaggca aaaaaatgat gaagaaatta aaaatatatg ctgggccgga
     6781 tcatccgcat caggctcaac aaccggaaga actggaagtt taaatttact taggaaagga
     6841 ggaatttaaa tggccgcaga agttcaatac tggggaaccg gtagaagaaa aactgccgta
     6901 gctagagtca ggttagtacc tggcgatggt aatatcttag taaacgatag agatgtgcaa
     6961 gattatttca accgtaaatc cttaattcaa gatttaatgt ctcctctgga gcttacaaat
     7021 acaactgata atttagatgt tttagttaat gtaaacggag gtggactttc tggtcaagca
     7081 ggtgcagtcc gtttaggtat tgcccgcgct ctacttaaag tagatggtga ttacagaaag
     7141 ccgcttaaag atgccggtta cctaaccaga gattcaagaa tgaaagaaag aagaaaatac
     7201 ggtcacaaga aagcccgtaa atctccacaa ttctcaaaga gataaaattt caaattcata
     7261 tattgataaa cacaaaaggg aggtctatcc tctctttttt tatttatata tattatttta
     7321 aaaaagagga ttattatttt atttcttgaa atttatatat aaataaatca taaagaggag
     7381 ggattgcagt gagtctgttg atggcaggtt tttcacccca tccacccctg gctattcctg
     7441 atattggtag ggataattta aagaaagtat ccgataccat agatagcctt aaagaacttg
     7501 gcagagaagt tgtagaaagt gatcccgaac tattgataac aatcagccca catggtaccg
     7561 ttatgcggga atcgatctca attattgatg aagatgttgt acgtggtgat tttggacagt
     7621 ttggctatag taatataagc tttgaaagaa aagtggatac ccatttcata gatattttaa
     7681 aaaagaatgc gtctaaagaa ggttttcata ccatcagttt aaaggataga gatggtacta
     7741 aactcgatca tggagtcctt gtaccactct attatatcca agaagccggt ctcaaggata
     7801 ttccgattgt acctattaat atggcctttt gggactatca tgaactttat gagttcggta
     7861 aaatcattaa ccaaactatt gaagaagaaa acatcgatgc ggttgccgtt gccagtgggg
     7921 acctctctca tcgtttaaaa ccaggcgccc cggccggtta tgatgaaaat gcggaagagt
     7981 ttgataagga gtttgtcaga ttaattgatg aaaaccaatt agctcagatc aaagatatgg
     8041 aaaaaggcct tattcacagg gctggagaat gtggacaccg cccgatggtc attttgtcag
     8101 gtattttatc cgaacacaag gtacagaccg atttaaaatc atatgaagga ccttttggtg
     8161 ttggttatgc cgtagccagt ttcaaaattc tcgcaggggg taattaggtt atggaagaag
     8221 aaatgggata tgtcgaatta gccagaaaaa ctattgaaaa ttatataaag gaaaaaagaa
     8281 agatagatcc accgcaatcc ggtaagtttc aaaaaagagc cggagccttt gtttctataa
     8341 aaaagagcgg ccaactaagg ggctgtattg gaactataga gcccacccgt ccctttttag
     8401 gccaggaaat tgtcgaaaat gcgatcagtg cctccacccg cgacccgcgt tttcaaccta
     8461 tcacggaaga tgaacttaat cagattgata tatctgttga tattctagga gaaaaggaac
     8521 ctgtagaaga tatttcacag ttggacccag aagagtatgg tgtgattgtg aagaaaaact
     8581 cccggaccgg tctgctttta cccaacctgg aaggggtaga tacagttgaa cgccagctcg
     8641 aaatagccag aaaaaaggcg ggtatttcag cccaggaaga ttatcaagtc ttaagattta
     8701 aggtggagcg ctatgaagaa taagaaaaag gaagccatgt tttatgaaaa aaaggaaggg
     8761 gaaaccgtcc agtgtcagtt atgtcctcat aactgtgtaa taaacgaggg caagagagga
     8821 atctgccggg ttagaattaa tgaagagggg actctttata cagaaaatta tggggagata
     8881 agttcaatgg gggttgaccc cattgagaaa aaaccgcttt atcattttta tccctcacaa
     8941 ggcatactct cactgggaac ttatggttgt aacttcaaat gcaagttctg ccaaaatttt
     9001 agaataagtc agcagcagcc taccactact taccagaccc cgtccgatat tattaaacag
     9061 gctaaagacc gtaatataat gggtattgca tatacttatt ccgaaccaat ggtatggtat
     9121 gaatttgtct atgaaaccgc aataaaggcc catgaagccg gtttaaacaa tgtgctggtc
     9181 actaatggat atcttaatca aaagccgctt aaaaagctta ctccctacat cgatgcagct
     9241 aatattgatc tgaagtctta ccataatgat ttctataaag agatctgtgg aggtaccctc
     9301 cagccggttt tagagaccat caaatacatg tacggtaaaa ctcatcttga attgacgact
     9361 ttaattgtca caaaccataa tgatggtcta gatgagttag aagacatatt caagtgggta
     9421 tctgatttaa atgaagatat accgatgcac ctttcccgct attttcctaa ctacaagatg
     9481 gatgaaccgg ccaccccgga aagaaagatg gaggcagcct atcagcttgc taaaaagtat
     9541 ttaaactatg tctatatcgg taatatgaga agtaaaaaag gtcagaatac ttactgtcca
     9601 gactgtggga cagaagtgat taccaggcat tattttaatg cccaaaacaa acttgaagag
     9661 ggcaaatgtc cggaatgcgg taagcagatc ttagagtact attaaataaa gttccaaccg
     9721 tagatataga aagtataaat caagtaaaag cctgccccaa aaaaggaagg cacgaggata
     9781 gatttttcag cccgttcata aagggtcacc gagattaagg caaagataaa gtagataatg
     9841 atcttatccg gtgagagatg gtagattaaa aaggagaaaa agagggctga cattaatttt
     9901 gaaactagct tgggtaattt ataattaaaa acttcataaa caaagaggtt cagcatcaac
     9961 tgttcactga aggcaatgat aaaaaatatg gggaagacca aaataagtgg taaagtactg
    10021 cgtacaaagg tttccatggt taaaacctta tagaggggtt taaaaatccg ggaaatattg
    10081 tccgtactta aagggaaatt gattaataag ataacaagac ccaataaaat aataaggtag
    10141 ataataagag aaattagttt cttagttaat gatttgaatg agatacctag ctctttaaaa
    10201 gataaatcat agataaaaac aaagtataag aagattagac taataaaaat aaggcgggct
    10261 gcaaagtgaa ataaaaggtc aaaaaaaccg cttaaagccg gtacctgcat aaaagaagcg
    10321 aagtcaaaga accggttaaa gattaaagta aaaaaccaaa gtgtatatat gataattata
    10381 tctttatagt tgattctatt tatccagttt gttttagaat tcatagggca atcacctctt
    10441 attattttct ttataaataa ttaaattcct gctttataaa tcagttaata acctatataa
    10501 tatataatga taagcggttt tgcaaaaaaa ggggtgggtt aaatttgatc tggtcagtaa
    10561 ttaccaatat aattgacctc ttcattgtag gagcaattat atattttctc ctcaacttaa
    10621 taaaagggac cagggctgtc cagttaatta aaggtctcgt actcattttc attgcttctg
    10681 tcgtaagcca gctcttgaac ttaaacacgg ttcactggtt tttacagagc gttttgacca
    10741 tgttaatagt tgccctgccg atcgtcttcc accctgaatt aagaagggcc ttagaacaat
    10801 tagggcgagg tggcttttta agtaaccaat tagtccataa atggtcagcc aaagatatta
    10861 atgaaattat tatggcgtcc gatgagttag cggaagaaaa gacaggtgcc ttgattgtgt
    10921 ttgaaaacga agtgggtcta agagactata tagaaaccgg caccgagatt aatgcccaaa
    10981 ttagtaaaga attaattgaa aacctctttt ataaatcagc ccctctccat gatggggcgg
    11041 tcattgtagg agaaggcagg gtaaaagcag ccggctgtct attaaatcta tctaataaac
    11101 ccagtataaa tcccaagtta ggtacaagac accgggcagc tattggaatt accgaggaat
    11161 cagatgcgat tgtgattgtt gtctccgaag aaagtggagt aatctcactc gcccaggatg
    11221 gagaacttat cagatatctt gataaatcta aattaagaga gatcctattt aaggaatttg
    11281 taccccgaaa gacaaatgac ttcttttcat aaagtggggg agcaaaaatg agagaattct
    11341 ataacaaata taagataata ataatagctg tcatgatagc atttgttctc tggctgtatg
    11401 ttaactacca ggaaggaaat ttacagggta gtttcgaatt cggcagcaat cagattagat
    11461 taaatataca atatgaaaat ttaacagata agctagttgt tttagatata tctgatcaga
    11521 ccgtcttgat tgagccggaa ggttacaggt tttttaatac ctatgggccg gaagactttg
    11581 aggcatatat agatttaaga gaggttcaga tcggagataa catcagaaaa gtaaatatta
    11641 gaactcccca gggagtccag ataaatacac aagaaccttc tatagtgagg gtaacaattg
    11701 cttcctatag ggaagaaggt tctcaacaag aagcacaaaa ctgacaatca aagaagttat
    11761 atcatattat taattagagg tgaattggat tgggtaaaat ttttggcaca gacggcgtaa
    11821 gaggtattgc aaatagagag ataacaccag agcttgcctt aaaattaggc aaggcaggtg
    11881 gactgttttt aaaagaaaaa agcaaaatga atggaaattt ttttgtccta attggtaaag
    11941 acaccagaat ttcaggcagt atgttagaat ctgccttaat tgcaggtttc aattcagtgg
    12001 gagtaaatat agtcaagttg ggtataatct ctactccagc tgtctccttt ttaactgata
    12061 aaacagatgc cattggggga gtgatgatct cagcttccca taatccaatt gaagataatg
    12121 gtattaaatt tttcagtgaa aagggcttca aattaaccga tcaggaagaa ctgcagatcg
    12181 aagaatatat tttcaatgaa aaagagatta ctaaacctat tgggactgaa gtaggagata
    12241 tcataaagag agaaaaactt attcaccagt atgtagatta tctagaagat actgttgact
    12301 ccaattttaa tgaccttaag atagttttgg actgtgcaaa tggggcggcc tatgaaatag
    12361 ctccccagat ttttaataaa ctaggggccg aagtaattgc agttaatgat aaaccagacg
    12421 gtagacagat taacttagac tgcggctcta ctaaaccgga gaccatctta gaggaaacca
    12481 aaaaatataa agctgattta ggcattgcct atgacggaga tgccgatagg ctgatcatgg
    12541 tcgatgaaaa agggaatctt atagatggcg accatataat ggccttttta gcccttaata
    12601 tgttaaagga agataagtta gataaaaaga cacttgtaac cacccgttat agtaatctgg
    12661 gtctagaaga acttctcaat gaaagaggag cccgggttgt aaaatgtaaa aacggggacc
    12721 gttatgttct tcagaccctt ttagagaacg gctataatct tgggggagaa aaatccggtc
    12781 atatcatcat gttagattat aataaaacag gtgacggtgt tttaacatct ctaaaagtgg
    12841 tcgactactt gcagaaatac ggtcagcctt taagccagat aaatgatata tttaaaccct
    12901 ggccgcagga attaattaat gtaagggtca aagataaaga aaaactttct acaaataaaa
    12961 ggataaatga gctaataaaa gaaatagaag atgattacag taagcaaggc aggatttttg
    13021 taagggcttc cggaacagaa cctgtgatta gagtaatgtt agaagcaaaa gatgaagcaa
    13081 tgatcagtaa atgggaagat aaaatcgctt ccacaattaa agaagagtta aattaaaaca
    13141 aaggaggtgt ttgaaaacta aatataatta aagcgcccgg actctgtaat ggagttgacg
    13201 aggtggggag taatcgaaat atcggcggat ctcctcaagg tttaagtatt aacctttaat
    13261 tggtttgaca aaacctgcaa gcaattgtgg ggacaaaaaa accagaaata cttatgaaag
    13321 caagtatgaa aacaatgaat ataatataaa taacggaggt aaatatatgt gcggtatagt
    13381 aggttatgta ggtgaaaaaa aagctgcttc aatcctttac gatggattga aaaggcttga
    13441 gtacagaggt tatgattcgt ctggaattgc agttaatgat ggtttaaata tagatgttgt
    13501 aaagaagaaa gggaagttag aacagttaaa gaaaaatatc aaaggcaatg taaatggtgg
    13561 aacaactgga attggacata caagatgggc tactcacggt cagccttctg acattaattc
    13621 ccatccccat agtgactgta aggataattt tgtaattgca cataatggta tcattgaaaa
    13681 ctttcaaaat ataaagaagt atcttaaaaa agaagggcat atattcaaat cagaaacaga
    13741 taccgaggtt atcgcccacc tcttagaaga ctattatgaa ggtgacattt taaaggcttt
    13801 aaaaaagaca attgaaagac tggaaggttc ctatgcttta actattcttt ccaaacatga
    13861 tccagataag atatatgtgg caagaaaaga tagcccatta attatcggga tcggggaaga
    13921 tgaaaattat atagcttccg atataccggc cttcctgggt gaaacaagag acttctatat
    13981 cctagaagac ggcgaatttg ctgtaatcaa taaagatgaa atagatatct ttgactttaa
    14041 cttagcaccc aaagagaaag aagtatacca tgtagactgg gaccccgaga tggcggaaaa
    14101 acagggttat gaccacttta tgttaaaaga aattcatgaa caacccgatg ccgtccgacg
    14161 gttgatggcc aaccgggtta caccggaggg aattaatctg gcagaattaa atctagataa
    14221 agaatttctg aataacttaa accaggtcca tctaatcgcc tgtgggacag cttatcactc
    14281 ctgtttaata ggtaaatata tgatggagga tctctccgaa cttagtgttg aggttgatgt
    14341 agcatctgaa tatagataca ggaatgtacc gattaaagaa aatgatttag tgattgcggt
    14401 aagccagtcc ggtgaaacct cagatacact ggccagtctc aggttagcta aagaaaaagg
    14461 agccaaggtt ttagccttta caaattcccg cggcagcagt attgctcggg aagctgatga
    14521 ggtaatgctt ttaaaagcgg gccccgaaat ttcggtggct tccacaaaag cctatctaaa
    14581 tatggtggta gctttttata tgttcagcct ctttttagca gataaacaga acaaaattgc
    14641 taataataga tatttagaga tgtcaaaaga tttaataaaa ctaccggacc ttttagatga
    14701 tgtaatcgaa aaaagtgaaa gagtaatgaa ggatagggcc gaccaatatg caaacagtga
    14761 gaatgccttc tttatcggcc gtcatacgga ctatacactg gccatggaag gagccttaaa
    14821 actaaaagag atatcatata tccacgctga ggcctatccg gccggagaac ttaaacacgg
    14881 taccctggct ttaattgaag aaggagtacc ggtcgtagct ttaggaaccc gcgaaactgt
    14941 ctttgaaaag accttaagta atataactga ggttaaggct agagggggag atgtaacagc
    15001 cattgtccat aaagatgcta aagatattga agatgtagta gaccatatag tctatatacc
    15061 ggaagtaaac gaactattta ccggagctct tgctatcgtg cctcttcagc tcttagcata
    15121 ctacacagcg ctgctgagag gctgtgacat tgaccaaccc agaaacctgg ctaaaagtgt
    15181 aacagttgag taattaagag aataagaaga gggggttaaa accccctttt tttgccttta
    15241 aaacttttta ttgttttcat aatattttta ataaaggaag aataaaaatg actgcaaata
    15301 aaaatggtaa acctaaaaaa aatgaagtta ttgctttagg ggtagatatt ttagaagtag
    15361 accggataaa agaaataatt gaaaagcggg gagataagtt tttaaagaga gtatttacac
    15421 ctaaagaaat aaaatactgt cagtcccaaa aaaggtcaat agaatcgttt gcagtccggt
    15481 tcagtgcaaa agaagcattt attaaggcga tcgaaagtga aataaatata ccttatcaag
    15541 atatcgaagt tgataaaaaa gccaacaaga aaccaaaaat aaatttgcat ggtaaagctt
    15601 tattggcagc cgaggaaaaa aatgttgccg aaattttggt ttctttaagt cacgagaaaa
    15661 attatgttac ggccaatatt attttaaagg ggagaatata atgattgtat taactccaga
    15721 agagatggct aaacttgatc aaaaggttat tgataatggt tatgaatccc tgatattgat
    15781 ggaaacagcc ggtagaggga catttcaatc cctgcaagaa aaatatgaca gagataacaa
    15841 aatattaatt ttagccggtt ccggtaataa tgggggagat ggacttgtaa tcggtcgatt
    15901 acttgatata tgtaaatata atgttaaaat aataatagta ggaaaccaga ataaattaaa
    15961 taaagatccc ttgacaaatt ataaaatatg tgaattaagg gatatggata ttcaattttt
    16021 cgaacaagat tcggatttac agctgataaa aaatgctata actgaatcag atgtgattgt
    16081 agacagttta ttaggcacag gtctcaaggg agatttaaga tctccctatt tagagatcgt
    16141 gaaaatgatt aacgacagta aaaacaaggt tttagctgtt gatataccaa gcggaatcaa
    16201 tggtaggaac ggtcaagtga tgaaagaggc tgtaaaggct gactatacag ttaccatggc
    16261 tttttctaag attgggcatc atctttatcc cggccgtgcg tatacgggtg aacttcagag
    16321 agtagatctc ggttttcccg aacatttaat cgaatatagt aaatataaac accatagttt
    16381 aaccttacaa gaagccggta acttattacc caaaagagct aaaaccgggc ataaaggcag
    16441 ttttggtaag gtattagtaa taggcggctc caaaggatta gaaggagccc cggccttaag
    16501 tgggaaaagt gccttgaaaa tgggaacagg gcttgtaaaa accatgatcc cgtctgaaat
    16561 cagttccact gtgagcagtt tttctccgga attaatttct gatgatttga ctatcgagaa
    16621 tatagaaaaa gatagtaaag attatgatgt aattgcctta ggacccggtt taggaatagg
    16681 tgaatttcaa aaagaaattg tttcttatct gataaacaag gccgaactac ctattgtaat
    16741 tgatgccgat ggacttaata atttaaattt aaaagaatta aaaaatgcta aaagcaatct
    16801 tgtcttgact ccccatccgg gagaatttgc cagacttatc ggcaaatcaa tcccagaagt
    16861 acaggcaaac aggctaaaat acgtacgaga atttgttgat aaatataagg taaatctagt
    16921 tttaaaagga gccagcagct tagtttcaga cccggccggc aatatctata taaatccaac
    16981 cggtaatgaa ggaatggcta ctgctggtag tggagatgtg ttaacaggta tcataagctc
    17041 acttatcggg caaggactca atacttttaa cagtgcagtt ttaggggttt atttacatgg
    17101 attagctgga gatatatcta cagacaagtt aggatcacat tcagttgtag ctcaaaattt
    17161 aatagaaaat atacctcctg ctatttcaaa aatcaaacgg gggtgttaaa atgattactg
    17221 ctaaagatat catgactact gatgtagtca cagtagatcc gaagactagt gtcaaagaag
    17281 cggctaatat tatgagtgaa aaaggtatta gcggtttacc agtagttcaa aatggagaac
    17341 tggtgggtat tgttacggaa aatgacttga taattaagga taagaaactt catttccccg
    17401 agtatatcaa tgttataggg ggcataatat atttagaaag ttataagaag ttcagggaag
    17461 aatttaaaaa atttactgct gtaaatgtag aagaattaat gacaaaagat gtgataacgg
    17521 ttagtcccga tgattcaatt gaggatatag caactataat gcaccagaaa aatgttaata
    17581 gattgcctgt tgtagacaat tcgggtcaat taattggaat cgtgacaaga gcgaatattg
    17641 taaaacacat tgcagtggaa gaataactga atataaggag agtttccatg ataatatcgg
    17701 ataaaacaat taaaaattta atagaggaag aaactttagt gatagaacca tttgaagact
    17761 atttaattca acccgcttcc atcgatctaa ggttaggtac caattacctt aaaattgatg
    17821 aaaatcagat ggaagtaatg actttagaag ataaattaaa ctacaatcag atcgacagtg
    17881 atgaaattgt aattccgccc aattcctttc tcttagctac cacagaagaa tatatccagt
    17941 tacccaataa tctaaccgct tttgtagaag ggagaagttc gataggaaga atgggcttgt
    18001 tcatacagaa tgcaggctgg gtggatcccg gcttttctgg caagataacg ctagaacttt
    18061 ttaacgccaa tcgtctgccg attagattaa aggcaggccg ccgaatatgt caattagtaa
    18121 ttgccgaaat ggaccaagaa gctgaatttc cctatcaggg gaagtaccaa ggtcaggata
    18181 ctgcagtagg aagtaaagtg tttgaagata atgagaactt taaagattaa agtttgtttg
    18241 aaataaaatt gaacaaaaca gcagacaata cgactaggag gtgttaatat gcagaatatc
    18301 attattcgtg atattgataa gcatattggt cctggcgata ttgttggtgc tttcataaat
    18361 gaagctaata ttaacagtaa tgatataggc aatattaata taagagataa catggcagaa
    18421 gttgaagtaa aaaatgaagt ttgtgataga gtagtaaata ttatggacaa taataaaatt
    18481 ggcggtgtta aggttcactt agaagtagat gatgatgctg aaaggcgaca aaaagtaacc
    18541 cgatattata ataaatttaa gcagttggta gaattagaaa gacaggaaga gatggaaaga
    18601 catgaaattg aaattaaacg tctctcctcg cgggctcgcg agaagaaagg tagggccctt
    18661 ttaaagatga gaggtaaagg tgatggtcaa acttatgacc ataaacctaa agtcaagttt
    18721 atgaaagaag aagtgggaga gaaactaccc gatactgaga ttagtattgg ggacttagta
    18781 atggtcagta aaaatagacc tttagatgat aataatccga cgggaactgt agcagaaaaa
    18841 actaattatt ccatgacagt tgtttttgat gaaaatccac ccggctttgt cttcggcgat
    18901 tctctaaggg ttgacctcta tgtaaacgat attacttttc agaggatgct agatgcctta
    18961 aagaactttc gaaatgcaaa aagcgagacc cgtttgggtg aattacagcg aaagatgctg
    19021 ggtttagaag aaataaactg gaaagatgag tcttttgatg agatagagtg ggttaacact
    19081 gagttaaacc aatcgcaaaa agaagcggta aaaagttctc taaaagcgaa agatttttcc
    19141 ttgattcagg gtccacctgg aaccgggaaa acaatgacag ctattgagat cattaatcag
    19201 ggagttaaag aagaaaaaga gatattagct actgctgatt caaatgtggc agttgataac
    19261 ctggtagaga gattggttaa tatggggacc gaagttttaa gactaggtca tcccatcaga
    19321 gtaacaccac ttttacgcaa gcatacttta gattataaga tattagatca tcccgactac
    19381 aaagaagccg aaagactaag agaaaaggcc caggatcttt tagatcaaca agatcaatac
    19441 caacaccccg gtaatagttt gagaaggggt atgagcgata aaatgatcaa agaaaaagct
    19501 gaacaagagc aaggggcccg tggtgtaaaa ccagccataa taaaagatat ggcaaagtgg
    19561 cttgagattc aggatgaaat agatggttta ttcaatcgta ttgaaaaact ggaacaaaag
    19621 gcgatcgaag atttattaga aacagctgaa gtaatctgta cgacaaattc tacagctggt
    19681 tcagaactac tgggtgaata tgattttgac ttaattgtga tcgatgaagc aacccaatct
    19741 actgaacctg cttctttaat accatttatc aaaggtgata aagtggtatt aatcggtgat
    19801 cataagcagc tgccgcccac catattaagt caaaaagcat ctgaacaggg cttaagtata
    19861 tccatgttcg aaaggcttta tgaaatatat ggtgatgaaa tcaggtcaat gttagaaatc
    19921 cagtatagaa tgcatgatga tatcatggac ttttccaacc atgaattcta taacggcaag
    19981 ttaaaaagtt cgcctgaggt agctgagcac accttaagag acttaggcta taatatggaa
    20041 ggaaaaaaat gctttacaga taaatcactc aaccctgatc agccgattgt ctttatagat
    20101 acggttgata tgacggctga agaaagatcg ctgaaaggct caaattctta tgataatccg
    20161 gttgagatgg aaatactgct tgatatcgtg gatgaggcct tgaaatcggc cttaaagccg
    20221 gaagatatag cagtaattgc tccttataaa gatcaggtgg atttattgaa aaagcatact
    20281 aatatagaga atttagaaat aaatactgta gatggatttc agggtagaga aaaggaaatt
    20341 gttttaatct ccctggtaag aagtaatgag agtaataata ttggtttttt aagagactta
    20401 aggaggttaa atgtagcctt aacccgggcc aaaagaaagt tagttgtgat cggagacagc
    20461 agtactattg ctaaaaatga gacctatcaa aatctattag attatatcga agaacgcggt
    20521 ctttattata cactgtaaat attgtaaaat tgataaattt aagatatact agagaaaaag
    20581 aagcaagtaa atcacaagag ggttcttcac agatggggag tgactcccca tctgttttta
    20641 aatattttaa ctcctggagg tgcggtgatt ggaagaagaa aaaaaagaaa ttacgattga
    20701 aatttccaag gggctgttaa aggatctgga tgattatatc aaataccgta gagtcagtag
    20761 agataagttt ataagtgatg ctatcaatca ttatttatgt gaacacaaaa agaaggaatt
    20821 aagggagcag ttgaaaaagg gatatataga gatgagcgag ttaaatctag aattagccca
    20881 tgaaggatta aaatgtgata gtaattcgat aaagatttat gaaaaatgtt tagtggagtg
    20941 tgaataaagt tatggattta aaaagaggag atgtatacta tgctgacctc aaccctgtgg
    21001 ttggttcaga acagggtggg gtcagacctg tattagtgat tcaaaatgat attggcaata
    21061 aatatagtcc gacaataatt gtagcggcaa ttacttccaa gattaataaa gctcagctgc
    21121 cgactcatgt agagatctca gctgatgatt ccagtcttga tagagattct gtaatattgt
    21181 tagagcagat tagaactatc gataaaaaaa gattaaaaag tgaagttgcc aaattaaatg
    21241 gcgatatctt ggaaagagta aatagtgcca ttgagattag tttaggttta gtcaatcttt
    21301 aatacataat tttttaaatt aaataagggg tgtacagatg tcagaatata tagccgatat
    21361 atatcgcagt aatctagttg aaagcagaca tagaggggat gttgccgtag tagatccata
    21421 cggtaaactt aaatatttta gtggtaaccc cgataaccaa acatatttaa ggtcagccgc
    21481 caaaccgttt caaattttac cagtgattaa ttctggtgca gctgactttt ttaattttac
    21541 aaaacaagaa atcgccgtaa tgagtgcttc tcacaacgga gaagaaaaac atgttaaaac
    21601 tgttagagaa atattagaaa aaatagaggt aagtgaagag aaattaaact gtggaattca
    21661 cgacccctat tttaaaaaag cagctcacaa gctttattta gacggtaata aaccaacaga
    21721 tatacataat aattgttccg gcaagcatgc tgccctgctt gccttatgta agtttaacga
    21781 ctgggattta gataactacc ttgcaaaaga acaccccgtt caacaactca tgctaactac
    21841 aatcagtgag gtttcagata tagaaagaac agatatttat ttaggtgaag acggttgtgg
    21901 ggtagttgtt tttggtctgc ccgtaaagaa gatggcttat gcttttgccc gtttggccaa
    21961 tcccgactat ttgcctaaca aatataagga agctgctcgg cgtattacga aaagcattaa
    22021 tgaccatcct gatatgattg ccggtaccaa gcggtttaat accgacttaa ttgaggtctt
    22081 gggtgataaa ttaaccggta agatgggggc agaaggtgtt ttttgtttcg gtttatataa
    22141 tggacccgga gtaagtgtaa aggtagagga tggtaattct agggcgctcc ctccggttgt
    22201 tatagatatt ttaaagactt tagattatct aactgaggaa gagttaagcc agttacaaag
    22261 atatgtaaga ccggtaataa aaaaccacca tgaccatgag gtgggctata ttaaatctat
    22321 attaaattta aaaaaggatg tgaaccgtta gaatgatttt gattaaagat gctcagattt
    22381 taactatggc cgataatgat tacaaacagg gttccatatt gattaaggac ggtaaaatag
    22441 caaaaatttc agaaaatatt gaaattaaag acgagtatga agttattgat gctaaagacc
    22501 agattgtgat gccgggagtc atcgatgccc atacccattt agggatcggc gaagaaggag
    22561 taggctggga aggtcaggac tacaatgaaa tgacagaccc cttcacaccc caattaaggg
    22621 ctatcgatgc agtaaacccc gatgattacg gcattaaaga agcattagaa catggtgtaa
    22681 caaccatcat gacaggtccc ggtagtgcta acgtggttgg cggcgaaagt atggtcatta
    22741 aaaccacggg taaaactata gatgaaatgg tcttaaaaaa cccggccgga atcaaggctg
    22801 cttttggaga aaatccgaaa agagtatata atgaacagaa aaaactaccc acaacccgca
    22861 tgggtattgc cgcagtgatg agagaaaatt taatgaaggc ggaagattat ctggctaaaa
    22921 aagaaaaagc tcaaaaagac gatgagcctt tcgatagaga tatcaagatg gaaagtttaa
    22981 tccgtattat taaaaaggaa ctacccttaa aagtacatgc ccatcgggct gatgatatca
    23041 tgaccgcaat caggatcgcc aaagagtttg atattgatat aaccttagaa cattgtactg
    23101 aaggccataa agtagcagat gaaattgctg aagctaatat accagcaatt gtcggtcctt
    23161 ccatgacagg tagagtcaag gttgaattaa gtgagttaag ctttaaaact gccggggttc
    23221 tggcgaaagc aggcgtaaag atagccttaa tgagcgacca tcctgtaatc cccactaaaa
    23281 atgctactat ttatgcagct ttagcgatca aaggtggttt agaggaaaaa gaagccctta
    23341 aagccattac cattaaccct gccgaaatat taggggtaga cgaccgggta ggaagtattg
    23401 aagagggcaa ggatgctgat ttaattattt ttaaaggcca tccccttgat atcagttccg
    23461 atattcaaag agtaattgtt aatggtgaag tagtaaaaag agaagaggat gaaaaataaa
    23521 aattatgaaa ctgaaagata ttatagataa attagaatta gaaggagttc atgagaataa
    23581 agagttatta gagcttgata taaaaaaagc ctgtggtgcc gatttaatga gtgatgtttt
    23641 agcattttcc gatgctaaga cgatgctgat taccggttta accaatcccc aggtgataag
    23701 aacggctgaa atgatcggaa ttaaagtaat catatttgtg agaggtaaat caccggttga
    23761 agataccgta aaattagcca aagagaataa catctcctta tatcaaacag aaaaaccgat
    23821 gtttcaaacc tgtggtattt tatataatct cggcctaaaa cccgaaaaaa tggttaaaat
    23881 agagtaggtg accgataagt tttgaaaaga aaaaagataa tagataggca aatagagaga
    23941 agagattttg caagtggtgg ggaagtgtcc agtaagttaa aaaaattact ccgtaaatta
    24001 ggggtaaata ataaggatat tagaagaact tccatcataa catatgaact tgaaatgaat
    24061 ctgatcatac attctgaagg cggccgatta aaggcctttg tcacccccaa atcgataact
    24121 atcttttccg aagataaagg acctggtatt gaaaatgtag ataaggcatt tgaacccggt
    24181 tattcaactg cttctgatga gataagagag atgggttttg gggccggcat gggtttaaat
    24241 aatgtaaaga gattttccga tgatctggaa gtagaaagtg aagttggcga atatactaag
    24301 ttaaaagcaa cgataaactt aaatcaataa tagcaggagt gtaacaatga gtgataaaca
    24361 ttctgtatta ctaaaagaag ataaatgtga aggctgtact aactgtgtaa aaaactgtcc
    24421 cactaaagct attagagttc atcagggtaa ggccactatc aaagaggacc tctgtattga
    24481 ctgtgccgaa tgtattagaa cctgtgaata ccatgcaaaa tatgcagaaa ctaatgatct
    24541 agccgaactg gcaaccaatg aatacaacat agctttatta gcgcccagtt tttacggtca
    24601 gtttgatagt tcctttgatc ctgaaaaaat taaaaaagca gtctataaac tcgggtttga
    24661 tgaagtctgg gatgtagcct atgcggcgga agcaatttca caaaagacat accagttttt
    24721 acaaaataat gatggcacct atatttccgc ttcctgtccc gtgattgtga ggttgataag
    24781 cttactttat cccgagcttt tagataatat cgtacccttt aattctcccg tggacttaat
    24841 ggcggactat gttaaaaata aagtggaagc agagggcaga gaacaatacg gtatgtattt
    24901 cataacaccc tgtccggcta aagtcactgc cattaaaaat cctttaggga gaagtgaatc
    24961 cagtttaacc ggggcgatcg cagtcgataa gatatatcct aaattacttg aattgatcaa
    25021 aggtgacaat aattttaagg aagttgaact taaacagaaa tatcagccct atatgggtat
    25081 ttcctgggga cagagcgggg gagaaaatga tattttaaat gaagaaggta taatagatac
    25141 cttgtctgtt tcaggtatac ataatattaa aaaagtgtta gaagaattaa cgaggggtaa
    25201 tatcaaagga ataaaatatc tagaactagt ggcctgtcca accggttgtg ttggagggat
    25261 ttttaatgtg aataatccct tccaggctaa atataatata aggatgaata taagaaatca
    25321 caaggagttt atagaccagg acctaaatga atacgacttc aaattagaag aaaagataaa
    25381 ggaaattggg gccgaaaagc tggatgaaga ctttgaaaca gctatcggca aattagaaga
    25441 actagaaaaa gaaattgaaa gactgccggg cttagactgt gcagcctgtg gtgcacctga
    25501 ttgtgaaacc tttgctgaag atgtggtaaa tggtctagca aaaaggtcgg actgtatttt
    25561 tgtcttaaga aaggaaattg gtgatttagc cgaccagctt tctaaattaa cccatgaact
    25621 gccaccggtg atgaacagtg aagaaaatca gaaaaaagat aaagaagagt aaagaggtga
    25681 tggaataatg cagttaaaag agataactga atctttgcat ttagaagttt taaatgaagc
    25741 cgatcttaca aaagaggtta agggctgtta tataggagac ctattaagta atgtgatggc
    25801 ccgggcgcgc gagggcgata tctgggtaac aatccagggc catcaaaaca tagtagcagt
    25861 agcacttttg gcagatgcag cagctgtggt agtagcagaa gaagctgaag ttgatgagaa
    25921 gtccatcaaa agagcagatg aaaaagatgt aaaccttttg cggtcaaagc tatctgccta
    25981 tgaactggcc caggaattta ctaaaatggg cgtttaagat gaaaaagtat cgcttggatt
    26041 tacatataca ttctgtatta tcaccctgtg ctgatatatt aatgacccct ggcaatatta
    26101 tcaaagaagc tgaaaagaaa gggattaata tcatatcaat tacagaccat aatgtatttg
    26161 gtaatgtgga aagcgcgata aaaatcgcgg aagaaaagga tataaccgtt ttgccgggga
    26221 tcgaagtcca gagcagagaa gagatccatc ttttaagcta ttttaacagc ttaaaggact
    26281 taaaagaata tggtaaaata atatatgaag gtttagaggg tatcaaaaat gatgaagaaa
    26341 aatttggagc ccagatctta gtagataaaa atgatgaatt tgttggtaag gaagagaagc
    26401 ttcttttaaa ttcaacaaaa tatagtgttg aggaacttgt taaaattaca tatcagaata
    26461 atggaattcc ggttccctct catgctgaac gaagtttcgg cataatccag aaccttggtt
    26521 ttattccagg ggatttggaa atttcttttg tagagatgaa ctttcataaa acatatgaag
    26581 attatttaaa agaatttccc tatttaaaaa agtataaatt attaaaaaac tctgacagtc
    26641 attatcttaa tcagataaaa cctactatgg agatatatct ggatgaaaag cctaattcag
    26701 agaagttttt ttcttattta aaatcccaca aaatagaaga atatcttcta taaaaagaaa
    26761 attttagcta ccaattgaca aagtgaaaaa attcacttat aatatatgtg tgtatgtgat
    26821 tttgctaact ttttaataaa ataatcaaat taagatagga ggtagacgga tgtcttgtag
    26881 atgtgatcaa aagaatatgg acaagtattt agccccctta aatgagatat taaaagagta
    26941 tacaaaagaa gaaaagtatt taatcccaat cttacaggat gctcaggacg aatacggtta
    27001 tttaccggaa gcagtcttaa aagaaattgc ctgtaatttg gatttatctt taagtcaagt
    27061 atttggtgtt gtgacatttt attctcagtt ccatttagag ccaagaggag aaaacattat
    27121 tagagtatgt acaggtactg cctgtcacgt aagaggtgga gctgaagttt tagaccagtt
    27181 aaaagaagaa cttaatatcg gcagtggtga aactaccgat gatctaacat ttaccttaga
    27241 aacagttgcc tgtattggtg cctgtgggct tgcccctgta attatggtaa atgatgatac
    27301 atatggtaga atgaaacccg gaaaagtatc tgaagtttta gcaaattacc aagaataatg
    27361 aaagaattag ccttacatat cttagatatt gctgaaaatt cgataaaggc tgagacagat
    27421 gagctaaaca tagaaattaa tgaagacacg cgagaaaatg agttcgagat tgtaattgaa
    27481 gatgacggta aagggataga aaaagaaaaa ttggaaaata ttactgatcc ttttgtgaca
    27541 agccgcaaga cgcggccggt aggtttaggg ctctccttat ttaaggcagc tgcccaaaga
    27601 tgtaatggta atttcgaaat cgattctgat agtaacggta caagcgtgaa agcagttttt
    27661 gagcatgatc atattgatag ggcaccctta ggtaatatgg cccaatcaat tgtatccata
    27721 ttactatggg acagtaatct tgagttaaca tatgaacata gatataatga aaaccaattt
    27781 atctttacta ccaaagagat taaaaaagag ttagaagatg taaagataac agacaataag
    27841 atattaaact ggctgaaaga ttatatagat gaaaatatta gagaaattcg cggaggtgaa
    27901 tttaattgaa gtcgttagaa gaattaaggg aactgcgtga aaaggttgaa aaggatatgc
    27961 agatgcgtaa agaaaccaat aaacccaaga taatcattgg aatggggacc tgtggaatag
    28021 cagcgggagc aagagagatc cttaaagctg ttatggaaga gatagataaa agggaccttg
    28081 atgtagaagt aactcaaacc ggttgtattg gcatgtgtga gaaagaacct ctaatggatg
    28141 tcaaactacc cggtaaagat agaattacat atggtaattt aacgcgagaa gatgtaaaaa
    28201 ggattgttgt taaccatgta ataaatggta atgtagtaga tgaactcgtt atagctagat
    28261 tagcagaata acttaaagga ggttaattac gttgaaggat acaatatata gatcccatgt
    28321 cctaatttgt actggaacgg gctgtgtatc atccggttct aaaaagatca aacatgaact
    28381 tcaggaccag ttggcagata aggacttatt ggatgaaatt aaaatagtag aaaccggctg
    28441 tcacggtttt tgtgaaaaag gaccgattat ggtcgtttat ccggaagggg ttttttactg
    28501 tcagttaaaa gaagaagatg tagccgaaat agtagatgaa catcttctaa aaggaagaat
    28561 tgtcgaaaga ctattattca aagaaccaat gacagaagaa agcatccctt cttatcaaga
    28621 tatagatttc tataaaaaac agcatagaat agccctggct aactgtggta agatcaatcc
    28681 cgaaagtata gaagaataca tagcaaataa tggttatgaa gcagccggtc aggctttaac
    28741 ttctatgact ccacaaaaaa taatagaaga agtcaaaaac tctggcctgc gcggccgggg
    28801 gggaggcggt tttccgaccg gtcttaaatg gcagtttgcc agagataatg aaagtgataa
    28861 aaaatatgta atctgtaatg cggatgaagg tgaccccggt gcctttatgg ataggagtat
    28921 cctggaaggt gacccacata gaataattga gggtatgata atagccggtt atgcgatcgg
    28981 tgcagatgaa ggatatgtat atgtgagagc agaatatccg cttgccattg aaagattgca
    29041 aaaagccatc aatgatgcag aagaattagg cttattaggt gaagatatct ttggttctgg
    29101 ttttaatttc aaattgaaca ttaaaaaagg agccggtgcc tttgtttgtg gagaagaaac
    29161 agccctgatg gcttccattg aaggtaaaag gggtatgcct aatcccagac caccttatcc
    29221 cgctgagaaa gggctctggg gtaaaccgac caatattaat aatgtagaaa cttatgctaa
    29281 tataccatac ataataagtg aaggctcaga ggttttctct tcgattggga ccgaaaatag
    29341 taagggtacg aaagtatttg cccttaccgg taagatcaaa aataccggtt tagttgaagt
    29401 accgatgggt actaccttaa aagaggttgt ttttgatatt ggaggcggcc tcaccgaaga
    29461 taagaagttt aaagccattc aaaccggtgg accatcgggt ggatgtatcc cggaagacta
    29521 tctagattta ccaattgatt atgattccct tatcgatgtg ggggcaatga tgggttccgg
    29581 tggtatggtt gtaatggatg aaagtacctg tatggtagat gtagctcgct tcttcttatc
    29641 ctttacccaa aatgaatctt gtggtaaatg tactccctgc cgggagggta caaagagaat
    29701 gttagaaata ttaaatagga tctgtgaagg ggaaggaaaa gaaggcgata ttaaattact
    29761 aaaagaatta ggagagcata ttagtgaaac cgctttatgt ggtctcggtc aaactgctcc
    29821 taatccggtc cttagtacca taaaatattt tagaaatgag tacgaagctc atatctttga
    29881 taataaatgt ccagctggcg tatgtcagga attagcatca gcttatacca ttaataaaga
    29941 gacatgtgtg ggctgtggtc agtgtgtaaa agtatgtccg gtcgatgcca ttaccaaagg
    30001 tgatggaaac tactatgtaa ttgatgatga gatatgtatt tcttgtggtg catgtgaaaa
    30061 gaaatgtcca gtcgatgcca tatcacaggg ttaaacaggt aaaaaggagg tttaaatact
    30121 aaatgggaaa agtaagttta actatagatg agcaaaatat agaagttgaa aaaggtacga
    30181 caatacttga agctgccaaa gaagtaggta tagatgtacc ttatttatgc taccatcctg
    30241 atttaacact gcacggtgcc tgtagagtct gtgttgtgga agatctggaa gataattctc
    30301 tggtagcatc ttgtgtgact cccgttacag aaggtatgga aatatctaca accagtatga
    30361 aagcaagaag agcgagaaga agaaatgtaa atttactgct agctaatcac cccaatgact
    30421 gtttgggttg tgatagaaac ggtagttgtg aattacagga tattacccat agtttaggta
    30481 taacaaggga agatgttgaa gaaattgcag gtgaagtgcg tgaggtagaa agagacgagg
    30541 tgggacccgc tttaaaaaga gatcccaata aatgtatttt atgtggtcgc tgtgtaaggg
    30601 tctgtgaaga agtacaggga gtatcggccc tacagttcag taataggggc tttgactcaa
    30661 ttgtaaccac agcttttgac ctcccacaga gtgagatcaa ttgtgctaac tgtggacagt
    30721 gtgcaacagt ctgtccggta ggtgccataa cagaggtcag tgaaattcag aaagtctggc
    30781 acgccttaga agatgaggat aaacatgtta tagcacaaac cgcaccttct atccaggcaa
    30841 caatcgggga agagttcggt tatgaacccg gtacagttgt gacagggcag ttggtatctg
    30901 ctttaaggaa gttaggtttt gacaaagtat tttcaactga atttaccgct gacttaacca
    30961 tattagaaga aggttcagag tttttagaaa gattaactgc caataagaac cttcctcaca
    31021 taacatcctg ttgtccgggt tgggttaaat acgctgaaca taattattcg gatttattga
    31081 accatctatc ttctgctaag tcaccacagc agatgttctc tgccttatca aaaacctact
    31141 atgcggaaaa agcagatata gatcccgaaa aaatttatac cgtatccatt atgccctgta
    31201 cagctaaaaa gttcgaaaaa gatagagatg aaattaatgc aacagagtat aaggatactg
    31261 atgccgtatt aacaacaagg gaacttgccc ggatgatcaa agaggtagga ttacagttta
    31321 aaaacttacc taatgaggag tatgatgaac tgatgggtga gcataccgga gctggtacta
    31381 tctttggaac caccggtggt gtagcggaag ctgccgtcag aactgtaaaa gaaaaattaa
    31441 ccggtgaaga gctagaaaga ttaaacttgg gctttagagg aattaataaa gctgaagttg
    31501 taataaatga ccaaattatt aaggtagcaa tcgccaatgg tctcagaaat gcagaaaaac
    31561 tattagatga agtaagagct ggcgaatctg aataccactt tatagaagta atggcctgcc
    31621 cttatggttg tgtgggcggt ggtggtcagc ctcttccgat tagtgaagaa cataaagaat
    31681 tacgaggaaa aggactggct aatgccgatg aaaagaatgt ggttagaaaa tcacatgaaa
    31741 atccgatgat caaaaaacta tatgaagaat atttagaaga accaaatggt gaaaaatccc
    31801 atcacttact ccacactact taccatgaaa gaccaaaaaa ctaacaataa aaaaattaaa
    31861 tacagcgaat tatagcccca tctcttatca caagaggtgg ggtttcttga tttaagcagt
    31921 atactcaagt ataatgttaa tagcttaaaa aatcttcttt ttgagttttg aggaagtgaa
    31981 taaattgaaa tatataacta acagcccgga agaaacaaag atgataggac aaaggatatc
    32041 tgaaataata aaagaagaca aaaattattt ttcccagata atacttttaa caggtgattt
    32101 gggagccggt aaaaccgttt tggttaaagg attagccgag ggtcttgata tcaactttaa
    32161 tattacaagt cccactttca atttgataaa tgaatatgaa ggagagcccg gcttaatcca
    32221 tatggacttt tatcggattg ataaaaaaga agaattactc cagttaggac tggaagaata
    32281 tatggcaatg gataatgtaa aagccatcga atggccacaa ttgatctttg attatctgca
    32341 ggctgaattt atttttataa agatcaaaaa tatggatgga gacaaacgca aaataattat
    32401 agaagcggaa ggaccaaaaa gtaaaaacat acttaaagga ctgaaagaca atgttagtat
    32461 tgggaattga tacaagcaca gatatatgtt cggtcggact tagtaataag gaagacttta
    32521 tcggagaaat taatataaaa ctaaaaagga gacacagtga acgtctcttg ccaattataa
    32581 agagaatatt ttcagaaaca ggttatgaaa tacaggattt agatggttta gctgttaccg
    32641 atggtccggg ttcttttacc ggtcttcgga tcggattgag tacagtgcag gccttcaaaa
    32701 gtgccttaaa tattccggta tattctgtct cttccttaga atatatggcc tattctgtaa
    32761 gtcaacatta tgagaattat ttgctagttc ctactattga tgcccgcaat aaaagggttt
    32821 atacttctgt ttttacaagt gaaccccaac aaaaactttt aaaaagaata agggatgata
    32881 aagccgtaga agttaaatca ttgattgaag acttaagaaa atataaaaaa ggtaaggtaa
    32941 tctttgggag tggcacagac agttattatg aaatctttaa aaaagcaaat ttagataata
    33001 ctaagataat atataagata agaaactccg gcggatataa tctggcatgt ttaggatata
    33061 agtacctttc agccggccag gatactgatt atagcgagct tgtgcccaag tatttaaaga
    33121 aaccccaggc gcgccttaac tgggaaaaaa agtatctgca ggaggattaa tatggattta
    33181 tgtcttgatg tgatgaagct ggatgattta ggggaagtaa ttaaaataga gcgagaagct
    33241 ttttcaaacc cgtggaatat gaaatttttt aaagaggaat taattcataa ctcctttgcc
    33301 ctctatctaa ccgctaaaaa tgataatgaa ctggtgggtt atgtgggttg ttggtttaaa
    33361 gaccatgcta atgaagtaca tatagttaat ttagcagtaa aaaagaccaa aaggagacag
    33421 ggtataggat cctatttaat caaagaaatt gttgatatgt caaaaaattt aaatgcagat
    33481 actttaactt tggaagtcag aattactaat aaagcggcca ttgatttata tatacaactc
    33541 ggttttacca aagcgggtct aacaccaaac tattatctcg ataatgatga agatgcatta
    33601 ctgatgaaaa aggagcttaa ttaagatgaa tgaagacatt aaaatattag cagtagagac
    33661 atcttgtgat gaaacagctg ctgcagttag taaaaatgga ttagatattt tagcaagtgt
    33721 agttgcttcc caggttgact ggcataaaaa atatgggggc gtagtaccgg aaattgcttc
    33781 ccgtaaacac atggaattta ttaatcccgt ggttgaagag gctttagaaa aagctgatat
    33841 aaaattagcg gaacttgatg cggttgctgc tacttatgga ccgggtttag tcggttcact
    33901 gttagttggg ttatcttatg ccaaggccct ggcccttgct ttaaataaac cctttatcgg
    33961 ggttaaccat attgcgggac atatttatgc caatttcatt tcgaaaaagg atatcaagac
    34021 cccggtcatc tgtttaacag tttcaggcgg tcataccgat ttgctatatt ttgaagaata
    34081 tggggactat gaaatcttag gtaggaccag agatgatgca gccggggaag catttgataa
    34141 gatagcccgt tatcttggtt taggttaccc cggtggacct attattgaaa aaagggcaaa
    34201 gctgggtaaa gaagatgcag taaaatttcc ccgccccttt attgataaag atacctatga
    34261 tttcagtttt agcggtctca aaactgctgt aataaattat attcacaacc aaaagcaaaa
    34321 gggtaatgag ctcgatatta atgatatagc agccagcttt cagcgggctg taattgatat
    34381 tttaactgcg aaaacagttc gggccgccct taataaaaag gtaaacagcg taattttatc
    34441 aggtggggta gcagccaata aaagattcag agaggtatta cgcgaaagat taaaaaaaga
    34501 aggaatacct ttatattatc ctgatttaga actctgtacc gataatgcgg ccatgatcag
    34561 tacagtagcc tattatcagt ataaaaaagg tgaatttgct gactttgctc taaatgctga
    34621 ccccaatctg cagttatagg tgataaaaat ggaaaagaat aaaagggaat attatttata
    34681 taaaagtgta caggatgata acattttgcc cgtaacttca aaatgtaatc tatcctgtat
    34741 tttttgtagc catcaacaaa atccaaaaga acttgaaata atcagttttg gggatctaaa
    34801 gcttaaaaaa ataaatgatt taatcgatta tataaatccc aatcaaccga ttattatggg
    34861 agaatcggct accaaaataa tcgaaggtga acccttgctc catcctgatt ttaaagaaat
    34921 aattaaaagg attaggacaa aatataatga tactgaaatt agaattacta ccaatggggc
    34981 tttattagat aagaatttaa taaatttctt tacaaaaata aaaaaaataa ctcttaatat
    35041 ttcggtaaac tatttagagc ccgagctgcg agaaaaggta atgggagata actgtaatca
    35101 aaacattaat acaaccttac aaaaactaaa atattcaaat attgaatata attttagtat
    35161 ggtggccctg cctcatcttt atggttatca aaggattaaa gaagaaatta ttaaaatggc
    35221 tgaatatgaa cctttaacta tcagagtttt catgccgggt tatacgagct taacagcggc
    35281 tgatttgaag tttgatcaca aagaagtcta tcaaaaatta tctaaaatgg tagatgagat
    35341 taatacgttc caaaaaacac cggttactat tgagccgccg atg
//
LOCUS       DATGCJ010000042         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_139102, whole genome shotgun sequence.
ACCESSION   DATGCJ010000042 DATGCJ010000000
VERSION     DATGCJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139102"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VKN64_02035"
     CDS             complement(<1..280)
                     /locus_tag="VKN64_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMA59043.1"
                     /translation="MYKYLTQGLLLGLAYVAPIGMQNLYVINTALSGNKKRTYQVAGF
                     TIFFDILLALACFFGIGLLLKNVPSLKSTILLVGSILVILIGFKLIKEA"
     gene            complement(323..919)
                     /locus_tag="VKN64_02040"
     CDS             complement(323..919)
                     /locus_tag="VKN64_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA59044.1"
                     /translation="MLLDILKKRKSVRKFSDKEITDDEIDYILEAGRLSPSVGNEQPW
                     KFGVVKDKSLIKKTAKIAYNQKWIESAELLIVLCTRIVNDREGGRDIQISRFPELKEK
                     IKNLDKEMYSKLNLEEHQTKIPGTHMVLAATEQGIGSTWISYFKVEELSDLLGLQENY
                     LPSEIIAFGYPKKERKRTVKKSIEEVVFFDDDFYNQNS"
     gene            complement(926..1570)
                     /locus_tag="VKN64_02045"
     CDS             complement(926..1570)
                     /locus_tag="VKN64_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59045.1"
                     /translation="MDKKINNIINNTYFIGGSPCSGKSTTAEMLSEKYNLEYYKIDDY
                     EFTHLDLAEKEKHPTMFKYKQMSWDEIWMRDPYFQAEEEYEFYRERFGFVIDGLKKYD
                     KNDKLIIEGAALLPELINGLPIDNNRVLYMVPEKEFQIKHYTKRSFKDQILKECSDPE
                     QAFANWMERDYIFAQMVKAQADNFGYKTIEVDGSNTIQKNVDLIVNHFKLNNWE"
     gene            complement(1563..>2064)
                     /locus_tag="VKN64_02050"
     CDS             complement(1563..>2064)
                     /locus_tag="VKN64_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59046.1"
                     /translation="NFDLIILACNCLYELATPEQQAKIIQNAYDSLNEGGFIFIDNDN
                     MEGKLDETWSEIGKEKKIFPNGTCQHGIEFKGSYKTSYVDRAKKIWRAERKVEIIYPD
                     GRKEVKRLNQQKHPVSADEVKQWLTENNFKIIEIYSDLEQNKDFYKGAKRATFIAKKE
                     KGEVSG"
BASE COUNT          624 a          397 c          252 g          791 t
ORIGIN      
        1 ccgcttcttt aattaattta aatccgatta agataactaa aatagagcca actaataaga
       61 tggtgctttt tagggaaggg acatttttaa gtaaaagacc aatcccaaaa aaacaggcta
      121 atgctaaaag gatatcaaaa aagatcgtaa aaccggctac ttgataagtc cttttcttat
      181 taccacttaa agctgtattt ataacataaa gattctgcat tccaatcggt gccacatatg
      241 ctaaccctaa taacaaaccc tgtgttaagt atttgtacat ttttagctct ccaattttta
      301 aaatttagct actataatct aattaactat tttgattata aaagtcatca tcaaaaaata
      361 ctacttcttc tatacttttc tttactgttc tctttctttc ctttttagga taaccaaagg
      421 caataatttc agatggcaaa taattttctt gtaatcctaa caaatcagat aattcttcta
      481 ccttaaaata tgaaatccag gtagaaccta tcccctgttc tgtggctgct aaaaccatgt
      541 gagttccggg tattttagtt tggtgttctt ctaaattcaa tttggaatac atttccttat
      601 ctaaattctt tattttttct ttaagttcag gaaatctact aatctgaata tctcgcccgc
      661 cttctcggtc attaacaatc ctggtacata agacaattaa taattcggca ctttcaatcc
      721 atttttgatt ataggctatc tttgctgttt ttttgattaa acttttatct ttgacaactc
      781 caaacttcca gggttgttca ttgcccacag agggagataa acggccggct tctaagatat
      841 aatcaatctc gtcatcggtg atctctttat ctgaaaattt gcggacactt tttcttttct
      901 ttaatatatc taaaagcata aatacctact cccagttatt taatttaaaa tgatttacta
      961 tcaggtcaac atttttttga atagtattac tgccatcaac ttctattgtt ttatatccga
     1021 agttatcagc ctgtgctttt accatctgag cgaatatata atctctttcc atccagtttg
     1081 caaaggcctg ttcaggatca gaacattctt ttaatatctg gtccttaaat gatctttttg
     1141 tataatgttt aatttgaaat tctttttcag gaaccatata tagtactcta ttattatcaa
     1201 tcggtaaacc attgatcagt tctggtaaaa gggctgctcc ttctataatc aacttatcat
     1261 ttttatcata tttttttaaa ccatcaataa caaacccaaa cctttcccgg taaaattcat
     1321 actcttcttc tgcctgaaag taaggatccc tcatccaaat ttcatcccag ctcatctgtt
     1381 tatatttaaa catagttgga tgtttttctt tttcagccaa atcaagatgt gtaaattcat
     1441 aatcatcaat tttataatac tctaaattat atttttcgct taacatctca gcagtagtac
     1501 tctttccact acagggagaa ccaccgataa aataggtgtt attaattata ttatttatct
     1561 ttttatccac ttacctcacc tttttctttt ttagctataa aagtagctct cttcgctcct
     1621 ttataaaaat ccttattttg ttcaagatct gaatatattt caataatttt aaagttgttt
     1681 tcggttaacc actgttttac ttcatcagct gaaaccggat gtttttgttg gtttaatctc
     1741 ttaacctctt ttcgaccatc cggatagatg atttctactt ttctttcagc ccgccaaatc
     1801 ttttttgctc gatctacata gctagtttta tatgaaccct taaattcaat cccatgttga
     1861 caggttccat tgggaaaaat ctttttttct ttaccgattt cagaccaggt ttcatctaat
     1921 ttaccttcca tattatcatt atctataaaa atgaaaccac cttcatttaa agaatcataa
     1981 gcattttgaa ttattttagc ctgctgttct ggtgtggcta attcataaag acagttgcag
     2041 gctaaaatta ttagatcaaa attt
//
LOCUS       DATGCJ010000043         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_496877, whole genome shotgun sequence.
ACCESSION   DATGCJ010000043 DATGCJ010000000
VERSION     DATGCJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496877"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VKN64_02055"
     CDS             <1..1680
                     /locus_tag="VKN64_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59047.1"
                     /translation="YKYFSVQFLHFVGALLALVPPLIIREIIDEAIPSGNLQNILMLV
                     ALGFGVYLSTNIIRYLRVYYGHKYAQYITRDMRNDLYNHYQNLSMNFHDNKKTGELMS
                     RIIDDLNRLQEFAHHGPEAVINSFVIIIGTVVILFTLSVRLAIVSLIFTPLLMIFVYY
                     FMKKMHKAFRRTRESKANMNDKLEDNLAGIKVIKAFNNEEYEMDNFSEKNTAHAKARM
                     KAIKYISILMPGSRMLNVIGLLAVLGYGGYLTSLGAITIGTIVAFYNYLERFREPLLR
                     LVQRAQGLSRFFASIERFFNHIDIKPDIKSKPGQLPAENIKGIVEFNDVHFSYDDEQV
                     LQGIDLTADKDQTIALVGPSGAGKTTIVRLLLRLYDIDEGSIKIDGKEITDYNIDQLR
                     NSMAMVMQEDFLFSTSVAENIAYGKPDATREEIISCAKKANAHQFITEQLSDGYDTEV
                     GQRGVKLSGGQRQRISIARAFLKDPRILILDEATSSVDLETEELIQEAVNEVTQNRTT
                     FIIAHRLSTIINADKILFIENGRLIEKGRHEELIDRDSHYKEFYSKQFQTA"
     regulatory      1795..1917
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1946..2473
                     /locus_tag="VKN64_02060"
     CDS             1946..2473
                     /locus_tag="VKN64_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HMA59048.1"
                     /translation="MLYNSQPIMRREVFTIKRYIKIIPFILIFILIFTYPIQQEEVLR
                     LDKYEDENIKKVYLIQENNEPYQLLQKVSVKGYKDEIVMFIQIDMQKESINKLEIIYQ
                     NETSHYGGHISEEWFLNRFEGKDTTLTLEIVKMSAKKEEEVVAVTGATISSQAVVRGV
                     NKVLSNYREIKNNIK"
     gene            2525..3595
                     /locus_tag="VKN64_02065"
     CDS             2525..3595
                     /locus_tag="VKN64_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMA59049.1"
                     /translation="MKKLSFIILLVFLITIMIFGTVVSAEDGEFVISNLKEKDITISV
                     EKLKELPVVEKDVTSVDSSGDEDDYTVKGALFSDFLESINASKEDLKAMQLIAGDGYS
                     IEIPEPVLKNRQIILAYEINGEPLPDKVKPVRVIIPEERAMYWIKNLTKINIIEMVKT
                     VDIDSLVVLETAVNQVGKVDYTYYESEDKAVNVANLVDQIGLNTDKRTIAFTAADGYE
                     KDEQVDIFRDGYIKITGDHAPMFLSPDIPKGMYVKDVLYVGYGSTSLASIDSAMKAFT
                     TTTIDGTEGITLAKVIKEAGLISADKYKLTASDGYSVEVSSKDMENGIVYLENGEMVR
                     CAFSDLSKKFWIKNLYKIEPIK"
     gene            3685..4233
                     /locus_tag="VKN64_02070"
     CDS             3685..4233
                     /locus_tag="VKN64_02070"
                     /inference="COORDINATES: protein motif:HMM:NF021348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HMA59050.1"
                     /translation="MLKRLLAGFSVYHLIIIALMAGLGVATKPIIVPLAHMITGPLYI
                     PGGVIAGGFYMMWLILGAGLVKKRGTATLISTVQAIMMVSLGIYGTHGLVSFFTYIIP
                     GLAVDTLLLFFRDPAKLTPCFLGGIVANLSGTFLVNIVFFRLPLIPLILALSSASLSG
                     GLGGIIAFNIIKQFSKYDLLEA"
     gene            4265..>5157
                     /locus_tag="VKN64_02075"
     CDS             4265..>5157
                     /locus_tag="VKN64_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59051.1"
                     /translation="MKRSNKLFVLTLIVLIFVLSTTVYSLDKKTGFIPNAYIQGDVKQ
                     VINLKELQGVTTKEIEYKNHKLKVYPLKIILDKAEPINEKYEIVFSAQDGLRAKISSD
                     KIAESYIHFSPAYGWEAINLNHPISSNIKLIESITVVTKGESNKNIFSIITPTENLLT
                     KTPGQLLTSSYLRKVVFEGESTQKIEGVNYSASIYSEKKVLQPEEMINKKIDSRTILF
                     NEQGKTLRYKGGYLELNKNFVNYINPDQKESLKNIRGIVLDAPLKSNKDTFYDTLHYL
                     ENDTRVLIVLLDGFGYHQFKY"
BASE COUNT         1811 a          746 c          976 g         1624 t
ORIGIN      
        1 tataaatatt tttctgtaca attcctgcat ttcgtgggag cactattagc tttagtacct
       61 ccattaatta ttagagaaat aattgatgaa gctataccga gcggtaattt acaaaatata
      121 ttgatgttag ttgctctcgg atttggggtc tatttaagta caaatattat aaggtatttg
      181 agagtttact acggccacaa atatgcgcaa tatataaccc gtgatatgcg taatgattta
      241 tataatcatt atcaaaattt atctatgaat tttcatgata acaaaaagac cggggaactc
      301 atgtccagga taattgatga tctcaatcgt ctgcaggagt tcgcccacca cggtcccgaa
      361 gcagtaatca actcatttgt cattattatc ggtacagtag ttatcttatt taccctaagt
      421 gtaagattag caattgttag tttaattttc acaccattat taatgatttt tgtctattat
      481 ttcatgaaaa agatgcataa ggcttttcgc cgtaccaggg aaagtaaagc aaatatgaac
      541 gacaagttag aagataactt agctggtatc aaagtaatta aggctttcaa taatgaagag
      601 tacgagatgg ataatttttc agagaaaaat acagcccatg ctaaagcaag gatgaaagcg
      661 atcaagtata ttagtatctt gatgcctggt tcccgcatgt taaatgtaat aggtttactg
      721 gctgttttag gttatggagg ttatttaact tctttaggag caataacgat cggtacgatc
      781 gttgcttttt ataactactt agaacgcttc agagaaccgc tcttaagact ggttcaaagg
      841 gcgcagggtt taagtagatt ctttgccagt atcgaaagat tttttaacca tattgatatt
      901 aaacccgata taaaaagtaa gccgggtcag ttacccgcgg agaatataaa aggtattgtt
      961 gaatttaatg atgttcactt ttcttatgac gatgaacagg ttttacaggg tatagacctg
     1021 acagcagata aagatcaaac tattgccctg gttggaccga gtggggcagg gaaaacgacg
     1081 attgtaaggc tgctgcttag actatatgat attgatgagg gatccattaa aatagatggt
     1141 aaagaaataa cagactataa tattgatcag ttaagaaatt caatggccat ggttatgcag
     1201 gaagactttt tattttcaac ttcagtagca gaaaacattg cctatggaaa acccgatgca
     1261 acccgtgaag aaatcatcag ctgtgccaaa aaagccaatg ctcatcaatt tataacagaa
     1321 cagctttcag atgggtatga caccgaggtc ggacagagag gagttaaatt atcgggtggt
     1381 caaagacaga ggatatcgat agcccgggca tttttaaaag acccccgtat tttaatcctt
     1441 gatgaggcaa catcctctgt agacttagaa acggaagaat tgattcagga agcagtaaat
     1501 gaagtaactc aaaacaggac gacctttatt atagcccaca gactttccac tataatcaat
     1561 gcagataaga tattatttat tgaaaacggc cgtttaatag aaaaaggaag gcacgaagaa
     1621 ttaatagacc gagattcaca ttataaagaa ttttattcta aacagtttca aacagcttaa
     1681 ttgatttaaa tagggtatct atacgttgag tataggtgcc tttttttaat ttattgtctc
     1741 caataattcc cagtattttg acaatttcga caattctcta tataattata aataagtaaa
     1801 taacagatcc gattcgtatt ttctaaggat tgtttatcta tgaaaatacg aacgataggg
     1861 ttgtataagg aaacttggca gcctcccatt gtggaaagga ctaaatattt gtttttaaca
     1921 agtccctttc cggggctttt ttcttttgtt atataattct caacccataa tgagaaggga
     1981 ggtatttacc ataaaaagat atattaaaat catccctttt atattaattt ttattttaat
     2041 ctttacctac ccaatccaac aagaagaggt cttacgatta gataaatatg aagatgaaaa
     2101 tattaaaaaa gtatatctaa ttcaagagaa taatgagcct taccaactac tacaaaaagt
     2161 ttctgtcaaa gggtataaag atgaaatcgt catgtttatt cagatagata tgcaaaaaga
     2221 atcaataaat aaattagaaa tcatttatca aaatgagact tcacattatg gcggtcacat
     2281 aagtgaagag tggtttttaa accgatttga aggcaaggat actactttaa ccttagaaat
     2341 agttaagatg tcagccaaga aggaagaaga agttgtcgct gtaacggggg cgacaattag
     2401 tagtcaggct gtggtaaggg gagtcaataa ggtattaagt aactataggg aaataaaaaa
     2461 taatataaag taaagtttta ataataattt tgttttaatc aaaaacaaaa ggaggatatt
     2521 aagaatgaag aaattgagtt ttattatctt acttgtattt ttaattacga ttatgatctt
     2581 tggtaccgta gtttctgcag aggatggtga gtttgtcatt tcaaatttaa aggaaaagga
     2641 tattacaatt agtgtagaaa aattaaaaga attacctgta gtcgaaaaag atgttacttc
     2701 tgtggattcc agcggagatg aagatgatta taccgtaaaa ggcgccttat tttcagattt
     2761 tttagaatcc atcaatgcct ctaaggaaga tttaaaagcc atgcagctca tagcgggtga
     2821 tggttattct atagaaattc cagaaccagt tttgaaaaac agacagatca ttttagctta
     2881 tgaaattaat ggtgaaccct taccagataa ggttaaaccg gttagagtga ttattccaga
     2941 agaaagggcc atgtactgga ttaaaaacct gacaaaaatc aatataattg aaatggtcaa
     3001 aaccgttgat atagattccc tagtagtttt agaaactgcc gtaaatcaag tgggaaaagt
     3061 agattataca tattacgaaa gtgaagataa agctgttaat gttgctaatt tggtggacca
     3121 gataggactt aatactgata aaagaactat tgccttcaca gctgctgatg gatatgaaaa
     3181 agatgaacaa gtggatattt ttagagatgg gtatataaaa ataaccggtg accatgcccc
     3241 catgttttta tcacccgata tccctaaagg tatgtatgta aaagatgtat tatatgtagg
     3301 atatggatca acttcacttg cttcaataga ttcagcaatg aaggcattta ccacgactac
     3361 cattgatggt actgaaggta ttactttagc taaagtaatt aaagaagccg gcttaatttc
     3421 agctgataaa tataaattaa cagcaagtga tggttattct gtggaagtta gtagcaaaga
     3481 tatggaaaac ggtatagtct atttagaaaa tggagaaatg gttagatgtg cattttcaga
     3541 tttatctaaa aaattctgga taaagaattt atataaaatt gaaccaatta aataagacgg
     3601 aagtctagat ggattaagaa ataatcaaaa atagaagatg ttacaattaa aatttatgtc
     3661 ttgtaaaaat atgaggtgaa tatagtgtta aaaagacttc tggctggttt ttctgtctac
     3721 catttaataa ttatagcgct aatggcgggg cttggtgttg cgaccaagcc tattatcgtc
     3781 cctttagctc atatgattac cggcccctta tatataccag gcggtgtaat cgccggcggt
     3841 ttttacatga tgtggttaat actcggggcc ggactggtta aaaaaagagg gactgccacc
     3901 ttaatctcaa ctgttcaggc tatcatgatg gtttcacttg gtatttacgg cacccatggt
     3961 ttagtcagtt tctttactta tattataccg ggtttggctg ttgatacttt attactattt
     4021 tttagagatc ccgccaaact gacaccttgt tttctaggtg ggattgttgc taatttaagt
     4081 ggtactttct tagttaatat tgtatttttt aggttaccat tgataccact aatacttgcc
     4141 ttaagcagtg catctctttc cggtggttta gggggcataa ttgcatttaa tataattaag
     4201 cagttctcta aatatgattt attggaagca tgataattga taaattgatg ggggtggttt
     4261 tattttgaaa agatcaaata agttatttgt attaacactt atagtattaa tctttgtttt
     4321 aagtactact gtctacagcc tggataagaa gaccggcttt attcccaatg cctatataca
     4381 gggagatgta aagcaggtaa taaatcttaa agaactgcaa ggagtcacta ccaaagaaat
     4441 agagtataag aatcataaac taaaagtcta tcctttaaaa atcattttag ataaggctga
     4501 acctattaat gaaaaatatg aaatagtttt ttctgcccag gatggcctac gggctaaaat
     4561 ttcttctgac aaaattgcag aaagttatat ccacttttcc cctgcctacg gttgggaagc
     4621 aattaattta aaccatccta tcagcagcaa tattaagtta atagaaagta taactgtagt
     4681 aaccaaggga gagtctaata aaaatatatt ttctataata actcccactg aaaatttatt
     4741 aactaaaact cccgggcagc ttttaacaag ttcttatctg agaaaagtag tttttgaagg
     4801 agaatctact cagaaaatag agggagtaaa ttattctgct tccatttact ctgaaaagaa
     4861 agttttacag ccggaagaaa tgataaataa aaagattgat tctagaacaa tattatttaa
     4921 tgaacagggt aaaacattaa gatacaaggg aggttatcta gagctaaaca agaattttgt
     4981 taattacatt aatcctgacc aaaaagaatc tttaaaaaat atacggggaa tagtattgga
     5041 cgctccttta aagagtaata aagatacttt ttatgataca ctacattatt tagaaaatga
     5101 tactagagta ctaattgtac ttttagatgg ttttggatat catcaattta agtattt
//
LOCUS       DATGCJ010000044        17962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_184209, whole genome shotgun sequence.
ACCESSION   DATGCJ010000044 DATGCJ010000000
VERSION     DATGCJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 17962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17962
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184209"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VKN64_02080"
     CDS             <1..1200
                     /locus_tag="VKN64_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HMA59052.1"
                     /translation="ANRGPHTVVTTFEDKGIGNVECTNCGQCILACPTGALHEVYHYE
                     HVWDALHDEDKHVVVQTAPATRVAIAEPFGADPGTIATGQMVAALRRLGFDKVFDTNF
                     TADLTIMEEGTELIERIKGDGTLPLFTSCSPGWIKFIEHQYPEYLDHISSCKSPQQMF
                     GAVAKTYYAERNNIDPEDLVVVSVMPCTAKKFELDREEMSEDVDYSLTTRELARMVKQ
                     AGIDILNLENEDYDELLGVSSGAADIFASTGGVMEAALRTAYELITGEELGKVDFEQV
                     RGLEGLKEGSVMINDMEVKVAVAHGLGNARRILERMKDGEEYHFVEFMACPGGCIGGG
                     GQPLPTDDNILEKRMQAIYEIDSSKKMRKSHENPFIKKLYDEYLGEPGSHRAHELLHT
                     EYKERGI"
     gene            1201..1821
                     /locus_tag="VKN64_02085"
     CDS             1201..1821
                     /locus_tag="VKN64_02085"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HMA59053.1"
                     /translation="MKKVPEPTIRRLFCYYRCLKNDKSDIIPDYVCSDTLGEKIGIKP
                     TLLRRDLSFFGHMGRKGKGYNKNKLKIRLEEIIGLNKEWEIALIGAGNLGKAILSYNQ
                     FREMGLYITEIFDNDLDKIGRKVNGYLVKNVKFIKQVLQEKGIKIVIIATSEDDVEGI
                     VHQLKKTNIEAIWNFTTAHIDLGDDVIVVKEDLCCSLGSIICNINK"
     gene            2014..3342
                     /gene="rlmD"
                     /locus_tag="VKN64_02090"
     CDS             2014..3342
                     /gene="rlmD"
                     /locus_tag="VKN64_02090"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMA59054.1"
                     /translation="MNNITYGGDGLGSLPSGKVIFVPKTIPGEKHLVEITKEKKSYAY
                     GKSVKILKESEDRIEPKCEIFQECGGCQWQHISYEAQKDFKEDILNYSLDRIGNLENI
                     KIEKILGVDYPWFYRNKSIMPLEVIDEEIKAGFYKRGTHQVIDNHTCYIQHQLINRIK
                     RYTVDLLNKYDDISIYNEEKHAGLLRHLHIRVGVCTNQALLTFITTDNDFDEINEIAE
                     KLMKKIPELKGIIRNINDEDTNVLIGDSSYTIKGQDFIYDYIGKRKYKITHDSFFQIN
                     TLQTEKLYNVVNEYADLGGNEIIVDAYSGIGSIATYLEEKYETVYGIESNKNAVEDGR
                     YNASLNKLENCEFIEGDVTEEIPKLMREDKKPDIVIFDPPRSGLNEDIITNVIDNKIN
                     KVIYVSCNPTTLARDLEELKQYYNISKVQPVDMFPQTYHIETVVLLERKK"
     gene            complement(3357..3794)
                     /locus_tag="VKN64_02095"
     CDS             complement(3357..3794)
                     /locus_tag="VKN64_02095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMA59055.1"
                     /translation="MENIDLKKLTLDDYPLTTYDKIRYGDTDRQDHVNNAVFATFLET
                     GRTELLYNPDQPLYSEGGSFVIAKLNLNLLAELKWPGKVEIGTAIKDIGSSSVKIVQS
                     LYQKGKVAATAETVIVHIDKEENKSKPLSEEAKVTLNKYKLEK"
     gene            3952..4224
                     /locus_tag="VKN64_02100"
     CDS             3952..4224
                     /locus_tag="VKN64_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59056.1"
                     /translation="MDAFLLVIFIIVGIAGLIFKVEAGAFIGLSLAPWQIIKLDLGQK
                     ISLYAIILSTIIGSIYFISISRWLLFLLYLLVEAYNYWGYQKRYSK"
     gene            4336..4611
                     /locus_tag="VKN64_02105"
     CDS             4336..4611
                     /locus_tag="VKN64_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HMA59057.1"
                     /translation="MPTYLYECENCGRFEEFQKITDNPLQKCPTCGEDVKKIIGSPGI
                     IFKGSGFYSTDNKSSSSNVNNGTSETKNKGSEKTEKTGKLDGKEKVS"
     gene            4730..5923
                     /locus_tag="VKN64_02110"
     CDS             4730..5923
                     /locus_tag="VKN64_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HMA59058.1"
                     /translation="MSKIEVRNLYKIFGNRPQKAIEMLKDGATKQEVLDKNNSTVGVN
                     DASFDVKEGEIFVIMGLSGSGKSTLLRCVNRLIEPTAGSIKVDGQEVRDFDKEELRQF
                     REEEFGMVFQNFALLPHRTVLENAMFGLEIQNVEKEERIRRTKEALEMVGLDGWEDQY
                     PEQLSGGMQQRVGLARALAVDPEIFLMDEPFSALDPLIKKEMQNKLLDLYEEMERTIL
                     FITHDLDEALKLGDRIAIMKEGKIIQIGDHEEILTNAANEYVEDFVEDVNRSRVLTAR
                     DIMTKPRELLYPQDGPRTALHKMKNNEIGSIFVVDRDKKYKGIVYMEDVVDLINNNDR
                     DFEDLIKDTTKADPEENLRELFGDIADLNVPLPVLDDDGKLSGIIVKTNVLANLASEG
                     QYDED"
     gene            5987..6922
                     /locus_tag="VKN64_02115"
     CDS             5987..6922
                     /locus_tag="VKN64_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59059.1"
                     /translation="MKKLLSILLVLVFTFSLVTVGAGNVQAADDSIDFGYVQWPGVTV
                     KTHVAKRVLEYLGYKTSMTSGSQAIVFKGMDTGDLDVFLGNWMPTMKIHFDKYSEKGT
                     VKNVRVNLHDVVYKTAVPEYVYEAGVKSFEDLNEHADKFNKTVYGIEPGNEGNLIIKK
                     AIENNTYNLGEWNLKASSTAGMLSAVKRAVNKEEWIAFNGWKPHYMNVMFDIRYLDDP
                     EGIWGSGERVLTVVRTGFEEENSNVYKFLEQFKVTAPMQNKWIDMYKRQEMDPQEVAE
                     KWIADNHDVVNQWVYGVQSTEGEMARKVIASKVSK"
     gene            7025..7864
                     /locus_tag="VKN64_02120"
     CDS             7025..7864
                     /locus_tag="VKN64_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMA59060.1"
                     /translation="MTVNSIIQLLTTKIPLGSWIENAVNFLINNFSGPLDGFANLIEW
                     MVNNFEAILSFAHPLIVIAIFAAIAWKLKNYKLSIFVALGLGLVLNLQMWGPLVTTLA
                     SIITSVVVALVIGIPIGILKAHIKTIDYITRPILDFMQTIPPFVYLIPALMFFGLGTV
                     SGVIATVVFAIAPPIRLTYLGIQQVDEELIEAGNAFGANWWEILTKIELPGAMPSIMM
                     GVNQCIMLSLSMVVIAAMIGAGGLGKPVLRSLSVVDIGLGFEAGLGVVIIAMVLDRMF
                     GRD"
     gene            8041..9183
                     /locus_tag="VKN64_02125"
     CDS             8041..9183
                     /locus_tag="VKN64_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HMA59061.1"
                     /translation="MDENIVAVSKCADYKEPNLNRALDLIFDQLGSIEKFVKQGDTVV
                     IKPNMLLGKKPEQVVTTHPKLIKQIIKRLQKIDCKIIIGDSPGGPFNEVRLKNIYKKV
                     GFYELGELENVELNYNTDSTKVSFEDGLLVKSFEIADFIKEADVIINMSKLKTHSFMK
                     YTGSVKNLFGTIPGMIKAEYHLRMPEANNFALMLVDLARLIKPQLNIMDAVYGMEGAG
                     PSAGEKRNFGYLMASTSPFSLDLAGTHLFNMKQKEVPTINQAIQKGITGTFDEIKIVG
                     DNLIPAKNTKVPKVNKSDTRNVSDLPHFLAKIINKLLKPRPVFNTDKCVKCGDCAANC
                     PADAIELDDYPEVDLEACIRCFCCQELCSYEAVDIKKPLLGKIFFN"
     gene            9207..10898
                     /locus_tag="VKN64_02130"
     CDS             9207..10898
                     /locus_tag="VKN64_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMA59062.1"
                     /translation="MNKKGRNIFDTIKNYKQNYFKSDLYSGLSVATLCIPQNMAYALI
                     AGLNPIYGLYTSIVSQIISTITGKSSYIIVGPTNLMAMAIASNLNYVAENNYLEMVVL
                     LTFLVGLFQIMAGIFRLGKLVRYVSHPVIIGLTSGAAIIIGVGQLKNLIGIDFGRTYN
                     VFIEFYKLIINLSTTNIISLLLGGSTIIIILLLKKSRFKVPSYLLGVFLITLITMVFG
                     LDPFVDTVGKLNVDSFSIGIPNLKLNDVLNTSSKALAVAIVGLIQTLAVVKSISVREN
                     EDLDINKEFISQGMMNFGLSFFNGFASSASFTNTFANAQSGAKTRISELISALAIIIF
                     ILFFGFFIRYIPIASLAGLVLIVAYNMIEIEELKELIKATKADTLIFVITFLATISLR
                     RIEYAIYLGVLLSIISVIKTSSEAKVGHLKYDEDSHGKIVQTVPEEIADDEYIILNLV
                     GDFTFSAADSFKYELEHVSNAGCGYIIRIRNIDNIDITSINELKKFINEVHHEDREVL
                     ISGVNQKKYEILKNLGIIDMVGEDNVFFENDMILSSTVDAVEYVENDENGDDDKS"
     gene            10961..12706
                     /locus_tag="VKN64_02135"
     CDS             10961..12706
                     /locus_tag="VKN64_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMA59063.1"
                     /translation="MKKSGRNILKTLKHYDKKNLKLDLSSGLSVAALSIPQNMAYALV
                     AGINPIFGLYTSIISKIISTFTSRSNYIIVGPTNLMAMAIASNLTNVQEGSYIEYVIL
                     LTFLVGIFQIIAGILKLGRLVRYVSHPVIVGLTTGAALLIGVSQLENLTGINVGRSYN
                     IFFELFYLFKKITSINFITLLFGIITIIVIIGIKKSQFKIPAYLFGVIIVTLISMVSG
                     LSDTMSTVGRINISSFSIHLPNLNPGLILNLSTKALAVAIVGLIQTLAVVKSISTRAN
                     EDLNINKEFVSQGLMNFGLSFFNGFASSASFTNTFTNYQSGAKTRISELISALSILIF
                     ILFFNFFIKFIPISSLAGLILIVAYNMVNIEGIKELIRATKADTLIFILTFLATIISP
                     RIEYAVYLGVLFSVISVMKDISKASVGHLKYDENSHAKIVQTVPEEVEDDEYIILDLI
                     GDFSFSAADSFKYELEHVSDAGCGYIIRIRNIDNIDITSINELKKFINEVQHQDREVL
                     ISGVNQNKYEILKNLGIIDMVGEDNIFFESDMVLSSTVDAVEQAENNNNNHKNDKNKN
                     KEKIDEKEEKKEEDN"
     gene            12777..13142
                     /locus_tag="VKN64_02140"
     CDS             12777..13142
                     /locus_tag="VKN64_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HMA59064.1"
                     /translation="MSVYDLANRLAKQIKNSDEYEDYLEKKKKVMQNEKTKEMLIDFQ
                     QEQMKLQAKQLSGEDLTEEEQEKLENLRDLVDINNDIKKYLQAEYRVNILLNDIQNII
                     FGDLELGIKEEDQNNKSEE"
     gene            complement(13180..13404)
                     /locus_tag="VKN64_02145"
     CDS             complement(13180..13404)
                     /locus_tag="VKN64_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMA59065.1"
                     /translation="MNKEEVQDVLDQIRPGLQADGGDVELIEVTDEGIVKVRLVGACH
                     GCPMSTLTLKNGIERTLKKKIPEVKEVVSV"
     gene            13513..16878
                     /locus_tag="VKN64_02150"
     CDS             13513..16878
                     /locus_tag="VKN64_02150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMA59066.1"
                     /translation="MVEKFVHLHTHTEYSLLDGALRVDNLIAQAKKFDMPAVAITDHG
                     VLYGMYEFYKKAKKNNIKPIIGCEVYVSPTDRFDKSSRQRYHLVLLAENNQGYKNLMK
                     IVSSSWLEGFYYKPRVDKDLLYKHREGIIALSACLQGELSQKILNNKNKAEIKESIKD
                     YQQIFGSDSYFLELQDHSLSEEKKVNATLIEIANEEDIPLVVSNDVHYQNKEDADVHD
                     VLLALQTGTTVNDEDRMTFPNDSFYFKNSLEMFKLFPDIKEAYKNTLKISERVNINIE
                     NDKFYLPNYPDTAKNESAYLRELCEKGLEEKGLADNKEAVERMKYEIKVIEDMGYVSY
                     FLIVWDFIKYANDNDIRVGPGRGSAAGSLVSYLLDITRINPLKYGLIFERFLNPERVT
                     MPDIDIDFDERRDEIIEYVKERYGKERVAQIGTFGTMAARGAVRDVGRALDIAYGKVD
                     KVAKMIPSQHGVNLEYALNNNQKLRERYENDKEVKKLIDYSREIEGLPRHISTHAAGV
                     IIGPEDLKNIIPLQLQDDNIITQLPMDDLEDMGLLKMDFLGLRNLTIINKSLDLIKKN
                     RNQSLDIENIPRDDSEVYEMLQEGQTLGVFQMESRLFQDLNQKLKPDRFEDIIALLAL
                     GRPGPLGSDIVDDYIKCRHGEKEPEYLHSKLKPILEETYGMILYQEQVMEIASKLAGY
                     SMGEADLLRRGMGKKKEELIKAERERFTKGAIENGIKEEIASEIFDQMEYFAGYGFNK
                     SHSAAYALLAYQTAYLKAKFPPEFMSALLSTVMNNLDKVSLYINEAKEMGLKVLPPDI
                     NESFFDFVPNKKGNIRFGLKAIKNVGTNSIETIVNSRKEEGYKTFEDFLEKIDIKKIN
                     VKTLEALIKSGCFDNLGVKRSQLLVKYEEVYEKISNSKKKVNSNQTSFFDMVEKEEDF
                     YDSKIEYPEIDEVDLDIKLEQEKEFLGIYVSGHPLDDYEEKIMMLTNASCNIDETSSK
                     NSLCFAGRILEVHQHYTKNNNLMAFVTVEDWDTSIDVVVFPDTYDNYKNALQQGKVIM
                     IAGNRSDSDSFIANKIIDLNLNMLEINLINLEIDQLNELRRFINSNKGQNPVIFNNST
                     KKVITDKEYWIDLSDATIKQLDDIISGQNYKIY"
     gene            16947..17702
                     /locus_tag="VKN64_02155"
     CDS             16947..17702
                     /locus_tag="VKN64_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HMA59067.1"
                     /translation="MNRFWELFIKGMPIGMSNTLPGISGGTIALVLKIYDELVHSIKK
                     IKIKFLIPVILGAVAGVFIGSSIIITLFNNYPIFVTAFLLGLILASSKVTFQEIDTFN
                     ILKVMFGIAGFIIAFMYSAEASNTVNINNISLIKYFAGGALGSVAMILPGISGGTILV
                     MMGLYHSVLAAISGLNLVIITVFGLGILSGLLIFSWLLSYLLDNYRSSLMALLTGLIL
                     GSTRSVIPAQFNIIAVLGLILGILVIYYLLMIE"
BASE COUNT         6428 a         2466 c         3453 g         5615 t
ORIGIN      
        1 gccaatagag gccctcatac tgtagtaacc acctttgaag ataagggtat aggtaatgta
       61 gaatgtacaa actgcggcca atgtatttta gcatgtccaa ccggagcttt acatgaagtt
      121 taccattatg aacatgtctg ggatgcatta catgatgaag ataaacatgt agttgtccag
      181 actgcgccgg caacaagggt agcaatagca gaaccctttg gggctgatcc cggtacaata
      241 gcaaccggtc aaatggtagc tgccttaaga agacttggtt ttgataaagt gtttgataca
      301 aacttcactg cggacttaac aataatggag gaaggaacag aattgatcga gagaattaaa
      361 ggagacggca ccttacccct ctttacttcc tgtagtccgg gctggattaa attcatagag
      421 catcaatatc cagaatattt agatcacata tcaagctgta aatcacctca acagatgttt
      481 ggggcagtag caaaaactta ttatgcagaa agaaataata ttgacccaga agatcttgtt
      541 gtagtatctg taatgccctg tactgctaaa aagttcgagt tagataggga agaaatgtca
      601 gaagatgttg attattcgtt aaccacaaga gaattagccc gtatggttaa acaggcaggt
      661 atcgatattt taaatctaga aaatgaagat tatgatgaat tactgggcgt ttcttcggga
      721 gcggccgata tatttgcctc taccggtggt gtgatggaag cagctctcag aactgcttat
      781 gagttaatta ccggtgaaga acttggtaaa gttgattttg aacaggttag aggacttgaa
      841 ggtttaaaag aaggctcagt tatgataaat gatatggagg ttaaagtagc tgtcgcccac
      901 ggcttaggta atgcaagacg aatattagag agaatgaagg atggagaaga gtatcacttt
      961 gttgaattca tggcctgtcc aggcggctgt ataggtggcg gcggacaacc actgcccact
     1021 gatgataata tacttgaaaa gagaatgcag gctatttatg aaatagatag ttccaagaaa
     1081 atgagaaaat cacatgaaaa tccatttatt aaaaagttat atgatgagta cttaggcgaa
     1141 cccggcagtc atagagccca tgaattactc catactgaat acaaggagcg ggggatttaa
     1201 atgaaaaaag tccccgagcc aactataaga agactatttt gttactacag atgtttaaaa
     1261 aatgataaat cagatatcat tccagattat gtatgctctg atacactcgg ggaaaaaata
     1321 ggaattaaac cgactttatt aagaagagac ttgtcatttt tcggacacat gggccgcaag
     1381 ggtaaaggat ataataaaaa caaactaaaa attagactag aagagattat aggattgaac
     1441 aaagaatggg aaattgccct tataggagct ggaaatcttg gcaaggcgat cttaagttat
     1501 aatcaattcc gagaaatggg cttatatatt actgaaatat ttgataatga tctagataaa
     1561 ataggtagga aagtgaatgg ttatctagtt aaaaatgtca aattcatcaa acaagtatta
     1621 caagaaaaag gtataaaaat agtaattatt gcaacatctg aggatgatgt ggaaggaatt
     1681 gtccatcaat taaaaaagac aaatatagag gcgatctgga actttacaac tgcccatatt
     1741 gatttaggag atgatgtaat agtagttaaa gaagacttat gctgcagttt gggtagtata
     1801 atatgtaata taaataaata aagaaataac ttagggaatg cttattatta gcattccctt
     1861 tttttaatga tgtcaactaa tatccagctt attgataagg ttaaattcct atgatataat
     1921 tacaaggtgt gataaaattg cctattattt cagctgggag ctgatattat ttctaaagag
     1981 agtattaaca agcataaaaa acaaataata gagataaata atataaccta tggtggagac
     2041 ggccttggca gtcttccctc aggcaaagta atttttgtcc ccaaaactat tccgggcgaa
     2101 aaacatttag tggagattac caaagaaaag aaaagttatg cctatggtaa atcggttaag
     2161 atattgaagg aatcagaaga cagaattgaa ccaaagtgtg aaatattcca ggaatgcggc
     2221 ggctgtcagt ggcagcatat tagttatgaa gcccaaaaag attttaaaga agatatacta
     2281 aattattcat tagatagaat aggtaatctt gaaaatatta aaatagaaaa aatattaggt
     2341 gttgattatc cctggtttta tcggaataaa tctatcatgc ctctcgaagt gatagatgaa
     2401 gagataaagg caggttttta taagagaggt acccatcagg ttatcgataa ccacacctgt
     2461 tatattcaac atcagttgat taacaggatc aaaagataca ctgtagactt attaaataaa
     2521 tatgatgata tatctatata taatgaagaa aaacacgctg gtttgttaag acatttacac
     2581 atcagggtag gggtttgtac caatcaagcc ttgcttactt ttattactac cgataatgat
     2641 tttgatgaga taaatgaaat agcagagaaa ttaatgaaaa aaattccgga actgaaagga
     2701 ataattagaa atattaatga tgaagatacc aatgtcctta taggagatag cagctatacg
     2761 ataaaaggac aggatttcat ctatgattat attggcaaaa gaaaatataa gataacacat
     2821 gattcctttt ttcagataaa caccttacaa acagagaagt tatataatgt agttaatgaa
     2881 tatgcagacc tgggcggaaa tgaaataatt gtagatgcgt attccggtat aggaagtata
     2941 gcaacttatc ttgaggagaa atatgagacc gtttatggaa ttgaatcaaa taaaaatgcg
     3001 gtagaagatg gacgatataa tgcttctctc aataaactag aaaactgtga atttattgaa
     3061 ggtgatgtaa cggaagaaat accaaaactg atgagagagg acaaaaaacc tgatattgtt
     3121 atctttgatc ctccccgcag tggtttaaat gaagatatta taactaatgt aatagataat
     3181 aaaatcaata aagtaatata tgtttcctgt aatccaacta ctttagccag ggatttagaa
     3241 gagctaaaac aatattataa tatttcaaaa gtacagccgg ttgatatgtt tccccagacc
     3301 taccatatag aaacagttgt attattagaa aggaaaaaat aaattaatta aagatactat
     3361 ttctctagtt tgtatttatt taaagtaact ttagcttctt ctgataatgg tttagattta
     3421 ttttcttctt tatctatatg aacgattact gtttcagctg tagcagcaac tttgcccttt
     3481 tgataaagac tttgcactat ttttacagaa ctactgccga tatctttgat agccgtgcca
     3541 atttcaactt taccaggcca ttttaactcc gctaagagat ttagattaag ttttgcaatt
     3601 acaaaagacc caccttcact atagaggggt tggtcaggat tatacagaag ttcagttcta
     3661 ccggtttcta gaaaagttgc aaaaacggca ttattaacat gatcctgtcg gtctgtatca
     3721 ccatatctta ttttatcata ggtagtaagg ggataatcat ctaaagtaag tttttttaaa
     3781 tctatatttt ccattattaa actcctttcg aaagaggatt tttgtataaa ttttcttgtc
     3841 atttactttt gctttaaatt taccatatac tttatttatt tgcaagagtt tcaaaaacaa
     3901 gtatcaatat acatataaaa aaattgttca atttttaagg aggtaagatt gatggatgca
     3961 tttttgctgg taatatttat aatagtaggt atagctggat tgatcttcaa agtagaggcc
     4021 ggagctttta ttggtttgag tttagcgccc tggcagataa ttaaattaga tttgggacaa
     4081 aaaataagtc tatatgccat tatcctttct accataatag gaagtatcta ctttatttca
     4141 atttcgagat ggctattatt tttactctac ttattagtag aagcatataa ttattggggc
     4201 tatcaaaaaa gatactccaa ataacttgac ttctgatttc agtgtgatat aaattaataa
     4261 tatagagagt aagcaaaaaa aaataaatat tgaattaaga tataaattgt aactgtaaca
     4321 aggaggtaat cagatatgcc aacttattta tatgagtgtg agaactgcgg cagattcgaa
     4381 gaatttcaaa aaattactga taatccttta caaaaatgtc ccacatgtgg agaagatgtt
     4441 aaaaaaataa ttggttctcc cggaatcata tttaaaggtt caggatttta ttctacggat
     4501 aataaaagta gcagctctaa tgtaaataat ggtacttcag agactaaaaa caaaggttct
     4561 gaaaaaaccg agaagacggg aaaattagac ggtaaagaaa aagtttctta atatagtttg
     4621 acaaactata aaatatttgt taatctttaa aacggttcag ctggtatgtg cttcacatat
     4681 tcgtctggac ctttttgttt gctcataaaa aatcagggga ggaaacaata tgtcaaaaat
     4741 agaagtacgg aatttatata aaatatttgg taacagaccc caaaaagcaa tagaaatgct
     4801 taaagatggt gctaccaaac aggaagtttt agacaaaaat aacagcactg ttggggtaaa
     4861 tgatgcaagc tttgatgtta aagaaggtga aatatttgta ataatgggac tatctggtag
     4921 tggtaagtct acactgctta gatgtgttaa tagactgatt gaacccaccg ccggttcaat
     4981 taaagttgat ggacaagaag tgcgggactt tgataaagaa gaactaaggc aatttagaga
     5041 agaagaattt ggaatggtct ttcagaactt tgcactctta ccgcatagaa ctgttctaga
     5101 gaatgcaatg tttggacttg aaattcaaaa tgttgaaaaa gaagaaagaa taagaagaac
     5161 aaaagaagca ctggaaatgg taggccttga cggctgggaa gatcaatatc cagaacagct
     5221 cagtggtgga atgcagcaga gagttggttt agcacgtgcg ttagctgttg atcctgagat
     5281 ctttttgatg gatgaaccat ttagtgcgct tgatcccctg attaaaaaag aaatgcagaa
     5341 taagttattg gatttatatg aagaaatgga aagaacaatt ctcttcatta ctcatgattt
     5401 agatgaagca ttaaaactcg gggatcgcat agctattatg aaagaaggaa aaatcatcca
     5461 aattggagac catgaagaaa tcttgaccaa tgctgccaat gaatatgtag aggactttgt
     5521 tgaagatgtt aatcgttcaa gagtattgac tgcaagggat attatgacta aaccgcgtga
     5581 attattatat ccacaggatg gaccacgaac agccctacac aagatgaaaa ataatgaaat
     5641 aggcagtatc tttgtagttg acagagataa aaagtataaa ggtatcgttt atatggaaga
     5701 tgttgttgat ttgattaata ataatgatag agactttgaa gatcttatca aagacacaac
     5761 aaaagctgac ccagaagaga acttaaggga actatttgga gatattgcag atcttaatgt
     5821 tccactacct gttttagatg acgatggaaa actatcaggt ataattgtaa aaactaacgt
     5881 tttagctaat ttagcttcag aaggacaata tgacgaagat taaaaaaata aggattaaga
     5941 attaaaaaag cacgtaaaaa attactctag gagggttttt tacaaaatga aaaaactatt
     6001 atcaattcta ttagtactag tctttacctt ttctctggta actgttggag caggcaatgt
     6061 acaagcagct gatgattcaa ttgactttgg ctatgttcag tggccgggag ttacagtaaa
     6121 aacccatgta gcaaaaagag ttttagaata tcttggttat aaaacaagta tgacaagtgg
     6181 atcacaggca atagtattta aaggtatgga taccggtgat ctggatgtct tcttaggaaa
     6241 ctggatgcct accatgaaga ttcactttga taaatatagc gaaaaaggca ctgttaaaaa
     6301 tgtaagagta aatctccatg atgtagtata caaaactgca gtacctgaat atgtatatga
     6361 agcaggagtt aaatcatttg aagacctaaa cgaacatgct gataagttta ataaaacagt
     6421 atatggtatt gaacctggta atgagggtaa tctcataatc aagaaagcaa ttgaaaataa
     6481 cacttataac ctaggtgaat ggaaccttaa agctagtagt acagctggta tgctttctgc
     6541 tgtaaaaaga gctgtaaata aggaagaatg gattgcattt aatggctgga aacctcacta
     6601 tatgaatgta atgtttgaca ttagatattt agatgatcct gaaggtattt ggggcagtgg
     6661 tgaacgtgtc ctaacagttg taagaactgg ctttgaagaa gaaaattcaa atgtatataa
     6721 attcttagaa cagtttaaag taactgctcc tatgcaaaat aaatggatag atatgtataa
     6781 gagacaagaa atggatccac aagaagttgc agaaaagtgg atagctgata accatgatgt
     6841 tgtaaatcaa tgggtatatg gagtacaatc cactgaagga gaaatggcaa gaaaagttat
     6901 tgcttctaaa gtaagcaaat aactattagt tgaaaatagt atttaataga tagattgttt
     6961 gtcttttaaa ctggcggcat tcttcattga atgccgctag taaaatatgt tgtaaaggag
     7021 gttgatgaca gttaatagta taatacagtt attaaccact aaaatacctt taggtagttg
     7081 gatagaaaat gcagtaaact ttttaataaa taatttcagt ggacctctag atggttttgc
     7141 taatttgatt gaatggatgg taaataattt tgaagctatc ttaagttttg cccatccatt
     7201 aatagtgata gcaatatttg cggcaatagc ctggaaatta aaaaattata aattatcaat
     7261 ttttgtagcg ctaggtttag gtttagtcct gaacttacaa atgtggggac cgctggtaac
     7321 taccttagct tccattatta catcagtggt tgtagcttta gtaatcggga ttccaattgg
     7381 tatcttgaaa gctcatatta aaacaattga ctatattaca agaccaatac tggactttat
     7441 gcagactata cctccatttg tatacctaat tcctgcttta atgttctttg gattaggtac
     7501 tgtttctggt gtaattgcga ctgttgtatt tgcaatagca ccaccgataa gattaactta
     7561 tcttggtatc caacaggttg atgaagagtt aatagaagca ggtaatgctt ttggtgctaa
     7621 ctggtgggaa atattgacca aaattgaact accaggagct atgccttcca ttatgatggg
     7681 tgtaaaccag tgtataatgc tttcactctc aatggttgta atcgcagcca tgatcggtgc
     7741 aggtggactt ggcaaacccg tcttgagatc actgtctgtt gtagatatcg gattaggatt
     7801 tgaagcaggt ttaggtgtag ttattatagc aatggtatta gatagaatgt tcggaagaga
     7861 ttaaataaaa atataaataa aatatactaa taaaatctgt ccttagtaag ggcagatttt
     7921 tttgtatatc tccctacttt tagaccgatc aaaacaagag ataaatatta tagtaaatta
     7981 attgaatata aaatttagct atgctattat taattaaaat aaaggaaact aggtgaaata
     8041 atggatgaaa atatagtagc ggtaagtaag tgtgcagatt ataaggaacc taatctaaat
     8101 agagccttag atcttatttt tgatcaactg ggtagtatag aaaagtttgt taaacaagga
     8161 gatacagtag taattaaacc taatatgctg ctggggaaaa aaccggagca agttgtaaca
     8221 acccacccta aattaatcaa acagatcata aagagactac aaaagataga ttgtaaaata
     8281 ataatcggag acagccccgg tggtcctttt aatgaagtta ggttaaaaaa catctataaa
     8341 aaggtcggtt tttatgagtt gggagaacta gaaaatgttg aattaaatta taatacggat
     8401 tcaacaaaag tatcctttga agatgggctt ttagttaaat catttgaaat agctgatttt
     8461 attaaagagg ccgatgtcat cattaatatg tcaaaattaa aaacacattc ttttatgaag
     8521 tataccggtt ctgtaaaaaa tctttttgga actattcccg gtatgattaa agctgaatac
     8581 catcttagga tgccggaagc aaataacttc gctttaatgc tggtcgattt ggccagatta
     8641 ataaaaccac agctcaatat tatggatgca gtatatggaa tggaaggggc cgggccctca
     8701 gcgggagaga agaggaattt tggttatctg atggcaagta cttctccttt ttcacttgat
     8761 ttagctggta cacatctttt taatatgaaa caaaaagaag taccaaccat aaatcaggct
     8821 attcaaaaag gtattacggg tactttcgat gaaattaaga tagtaggtga taacttaata
     8881 ccagctaaaa atacaaaagt acctaaagtt aataaatcag atactagaaa tgtcagtgat
     8941 cttccccatt ttttagcaaa gataattaat aagttattga aaccacgtcc tgtctttaat
     9001 actgataaat gtgtaaagtg tggagattgt gctgctaatt gtcccgctga tgctattgaa
     9061 ttagacgatt atcccgaagt tgatcttgaa gcctgtatta gatgtttttg ctgtcaggag
     9121 ttatgttctt atgaagcggt tgatattaaa aagcctttat tagggaaaat attttttaat
     9181 taagataaaa ttagatggtg aatataatga ataaaaaagg cagaaatatt tttgatacca
     9241 taaaaaacta caaacaaaat tactttaaat ccgacttata ttcgggttta tcagttgcca
     9301 ctctctgcat acctcaaaat atggcctatg ccctaattgc aggactaaac cctatttatg
     9361 gtctatatac ttcgatcgtt tctcaaataa tttcgacaat aactggtaaa tctagttata
     9421 taatagtcgg tccaactaat ttaatggcca tggctatagc gagtaactta aattatgtgg
     9481 cagaaaataa ttatctggaa atggtcgtgt tattaacatt tctagtcgga ctttttcaga
     9541 tcatggcagg tatatttagg ttgggtaaat tagttcgata tgtttctcat ccggtaataa
     9601 tcggtttaac ttcaggggca gctattatta ttggggttgg acagttaaaa aatttaattg
     9661 gtattgattt tggtcgcact tataatgtat ttattgaatt ttacaaatta atcataaatt
     9721 tgagtacgac aaatattatt tcattattat taggtggttc aactataata ataattttac
     9781 tattaaaaaa gagccggttt aaagtacctt cttatttatt aggagtattt ttaattacct
     9841 taataaccat ggtattcgga ttggatccct ttgtggatac agttggtaaa ttaaatgtag
     9901 atagctttag tattgggatt ccgaacttaa agttaaatga tgttttaaac acatcttcaa
     9961 aggctttagc tgtagctatt gtaggtttaa ttcaaaccct ggccgtcgtt aaatcaattt
    10021 cagtgagaga aaatgaggat ttagatatca ataaagaatt tattagtcaa ggtatgatga
    10081 actttggttt atctttcttt aatggttttg ctagttcggc ctcttttact aacacttttg
    10141 ctaatgctca aagtggggct aagaccagaa tttcagaact gatttctgct ttagctataa
    10201 taatatttat attatttttt ggatttttca ttagatatat tccgattgcc tccctggcag
    10261 ggttggttct aattgtcgcc tataatatga tagaaataga ggaattaaag gaattaatta
    10321 aggctacaaa agctgatact ttgatttttg taataacctt tttagcaact attagtcttc
    10381 gcagaattga atatgctatt tatcttgggg tattattatc tataatttca gtgataaaga
    10441 caagttctga agctaaagtt ggacacctca aatatgatga agattctcat ggtaaaatag
    10501 tacaaactgt accagaagag atcgccgatg atgagtatat catattgaac ttagtgggag
    10561 attttacttt cagtgcagcc gatagtttca aatatgaact agaacatgta tctaatgcag
    10621 gctgtggtta tataattagg atcagaaata ttgacaatat agatataaca tcgattaatg
    10681 aactaaagaa attcattaat gaagtccacc atgaagatag agaagtattg atttcgggtg
    10741 ttaaccagaa aaaatatgaa atccttaaaa acttaggtat aatagatatg gtgggagaag
    10801 ataatgtctt ctttgaaaat gatatgattt tgtcttccac ggtagatgcc gttgagtatg
    10861 tggagaatga tgaaaatggt gatgatgata aatcgtaata aaaaattttt aatttttagt
    10921 ttcaatgagt aaaattcatg aaaaacaatg gtgaattatt atgaaaaagt caggtagaaa
    10981 tattttaaaa actttaaaac attatgataa aaagaacctg aaattagatt tatcttcagg
    11041 attatcagtg gctgccttat caattccaca aaatatggcc tatgctttag tagccggtat
    11101 aaatcctatt tttggacttt acacttctat tatttccaag atcatctcca cttttaccag
    11161 tagatctaac tatataatag tagggcctac caacttgatg gccatggcga tagctagtaa
    11221 cttgactaat gtgcaggaag gtagttacat tgaatatgtt atcttattaa cctttttagt
    11281 aggtattttt cagataatag ctggtatatt aaaacttggt agattagtca gatatgtttc
    11341 tcaccccgtc attgtgggtt taacaacagg agcagccctg ctcatcgggg taagtcagct
    11401 agaaaattta acgggtatta atgttggtag aagttataat atatttttcg aattatttta
    11461 tttgttcaaa aaaattacct caattaattt catcacttta ttatttggta tcataacgat
    11521 catagtaata ataggtatta agaaaagtca atttaaaatt cctgcctatt tatttggtgt
    11581 tattatagtg actttgatta gtatggtttc gggtttaagt gataccatga gtacggtagg
    11641 aagaataaat ataagtagtt ttagtattca tttaccaaat ttaaaccctg gattaatatt
    11701 aaatctatct acaaaagctt tagcagttgc tatagtagga ttgattcaaa ctttggcggt
    11761 agtaaaatcg atatctacga gggccaatga agatttaaat attaataaag aatttgtgag
    11821 tcaaggtctt atgaactttg gattatcttt ttttaatggt tttgctagtt ctgcttcttt
    11881 tacaaatact tttacaaatt accagagcgg agcaaagacc cgcatatcag aattgatatc
    11941 tgctttatca atcctaattt ttatcctatt tttcaatttc tttattaaat ttatacctat
    12001 ttcttccctg gccggcttaa tcttaatagt tgcttataat atggtaaata tagagggaat
    12061 aaaagaactt attcgtgcaa ctaaggctga tactttaatt tttatattaa catttttagc
    12121 tactattatt tcgccccgga tagaatatgc tgtatattta ggtgtattgt tttctgtaat
    12181 ttctgtgatg aaggatatct caaaagccag tgtgggtcat ttaaaatatg acgaaaactc
    12241 ccatgcgaaa atagtccaaa cagtacccga agaggtagaa gatgatgaat atataatttt
    12301 agatctaatt ggggattttt cttttagtgc agccgatagt tttaaatatg aattagaaca
    12361 tgtatctgat gccggctgcg gctatataat aaggatcagg aatatcgata atattgacat
    12421 cacctctatt aatgaactta agaagtttat taatgaggta cagcatcaag atagggaggt
    12481 attgatttca ggggttaatc agaacaaata tgaaatactc aaaaatttag gaattataga
    12541 tatggtaggc gaggataata tcttttttga aagtgatatg gtactttcgt ctactgttga
    12601 tgcagtagaa caggcagaaa acaacaataa taatcacaaa aatgataaaa acaagaataa
    12661 agaaaagata gatgaaaaag aagaaaaaaa agaggaagat aactaaattt acaaaaatat
    12721 ctactttatg atattatgta gataagtaaa atgaaagcat aaaaggagga ataaggatgt
    12781 ctgtatatga tcttgctaat agattagcta agcaaattaa aaattctgat gaatatgaag
    12841 attatcttga aaagaagaaa aaagtaatgc aaaatgaaaa aacaaaagaa atgttaatag
    12901 actttcaaca agaacagatg aaattacagg caaaacaact ttctggggaa gatttaacgg
    12961 aagaagaaca agaaaaattg gaaaatttaa gagacctagt cgatattaac aatgatatta
    13021 aaaaatattt acaggccgaa tatagggtaa atattttatt aaatgatatt caaaatataa
    13081 tttttggcga tcttgaactt ggcattaaag aagaggatca aaataataaa tcagaagaat
    13141 aaaaataaag caccctgttt tagggtgctt attgtttgat tacacagata ctacttcttt
    13201 tacttctggt attttctttt tcaaagttct ttctatacca ttttttaaag tcaaagtaga
    13261 cattgggcag ccgtgacaag cgcctacaag cctaacttta actataccct cgtcagtcac
    13321 ttctataagt tctacatcgc caccatcggc ttgaaggcct ggtctgattt gatctagaac
    13381 gtcttgaact tcttctttat tcacagatta cacctccata taataatcta tttgtattat
    13441 aacatattat agaaattata caataataat tataaaatta atcaacttta tttagattgg
    13501 agagagatca aaatggttga aaaatttgtt catttacata cccataccga atacagccta
    13561 cttgatgggg ccctaagagt tgataattta attgcccagg ctaaaaagtt cgatatgccg
    13621 gccgtggcaa ttactgacca tggggtcttg tatggtatgt atgaatttta caaaaaagca
    13681 aaaaagaata atataaaacc gattatcgga tgtgaagttt atgtatctcc caccgataga
    13741 tttgataaat ccagccggca aagataccat cttgttctac ttgcggaaaa taatcaaggt
    13801 tataaaaatt taatgaaaat agtatcttcc tcatggctgg aaggctttta ttacaaaccg
    13861 agagtggata aagacctatt atataaacat agagaaggta ttatcgcctt atcagcctgt
    13921 ctacagggag aactttccca aaagatatta aataataaga ataaagccga gataaaagaa
    13981 tctattaaag attaccaaca gatttttggg tcagacagct atttcctaga gctgcaggac
    14041 catagtctgt ctgaagaaaa gaaagttaat gccaccttga ttgaaattgc taatgaagag
    14101 gatattccct tagtggtaag taatgatgtc cactatcaaa ataaagagga tgcagatgtt
    14161 catgatgttt tattagcctt acaaactggg acaactgtca atgatgaaga tagaatgact
    14221 tttcccaatg atagcttcta ttttaaaaat tccttagaaa tgtttaagtt attcccagat
    14281 attaaagaag cctataaaaa cacccttaag attagtgagc gggttaatat taatattgaa
    14341 aatgataaat tttatctgcc caattatccc gacactgcca aaaatgagtc agcctactta
    14401 cgagaattat gtgaaaaagg tttagaagaa aaaggtcttg ccgataataa agaagcagtt
    14461 gagagaatga aatatgaaat taaggtaatt gaggatatgg ggtatgtatc atatttttta
    14521 atcgtctggg attttattaa atatgcaaat gataatgata ttagagttgg tcccggccgc
    14581 ggttcggccg ccggttcttt agtttcatat ctattagata ttacccggat taacccctta
    14641 aaatatggac taatatttga gagattttta aaccctgaac gggtaacaat gcctgatata
    14701 gatattgatt ttgatgagag acgcgatgaa ataatagaat atgtaaaaga aagatacggc
    14761 aaggaaaggg tggcccagat cggcaccttt ggaaccatgg cagcccgggg tgcggtaaga
    14821 gatgtgggta gggcattgga tatcgcctat ggtaaggtgg ataaagtagc taagatgatc
    14881 ccttcccaac acggtgttaa tcttgaatat gctctaaata ataatcaaaa attaagagaa
    14941 agatatgaaa atgataagga agttaaaaag cttatagatt attcccgtga aatcgaaggc
    15001 ttacccaggc atatttctac tcatgccgcc ggagtaataa ttggaccaga agatttaaaa
    15061 aatataatac cgcttcagct tcaggatgat aatataataa cccaactgcc catggatgat
    15121 ctagaggata tggggctttt aaagatggac tttttgggtc ttagaaactt aaccattatt
    15181 aataaatcgc ttgatttaat caaaaaaaat agaaatcaat ccttagatat tgagaatatc
    15241 cctcgtgatg acagtgaagt atatgaaatg ttacaagagg gtcaaaccct cggggtattc
    15301 cagatggaat caagattatt tcaggattta aatcagaaat taaaaccgga tcgatttgaa
    15361 gatattattg ccctattggc tttaggccgt cccggtccgc tcggtagtga tatagttgat
    15421 gattatataa aatgtcgtca tggtgaaaaa gaaccggaat acttgcattc caaattaaag
    15481 ccgattttag aagaaaccta cggcatgatt ctctatcagg aacaggtcat ggaaattgcc
    15541 agtaaactag caggttattc gatgggagaa gcggaccttt taagaagggg catgggtaaa
    15601 aagaaagaag aattaattaa agcggaaaga gaaagattta ctaaaggtgc gatagagaac
    15661 ggcataaaag aagagatcgc cagtgaaata tttgaccaga tggaatactt tgccggctac
    15721 ggctttaata aatcacacag tgccgcctat gctttacttg cctatcagac tgcttatttg
    15781 aaggcgaaat ttccaccgga atttatgtca gccttgttat caactgtgat gaataatctg
    15841 gataaggttt ctttatacat taatgaggca aaggagatgg gtctcaaggt attaccacct
    15901 gatataaatg aaagtttctt tgattttgtt cccaataaaa aaggcaatat tagatttggt
    15961 ttaaaagcga ttaaaaatgt tggtaccaat tcgatcgaaa cgatagtaaa ctcgcgaaaa
    16021 gaagaagggt ataagacttt tgaagatttt cttgaaaaaa tagatattaa aaaaatcaat
    16081 gtaaaaacac tggaagcttt aattaaatcg ggctgttttg acaatctggg tgtaaaaagg
    16141 tcccaattgt tagtcaaata tgaagaagta tatgagaaga tatcgaacag caagaaaaaa
    16201 gttaattcga atcaaacctc cttctttgat atggtagaga aggaagaaga tttttatgat
    16261 agtaagattg aatatcctga aattgatgaa gtggatcttg atattaaatt agaacaggaa
    16321 aaagaattct tgggtatcta tgtttccgga caccccttag atgactatga agaaaaaata
    16381 atgatgttga ccaatgccag ctgtaatatt gatgaaacga gcagtaaaaa tagtctttgc
    16441 ttcgcgggcc gaatattaga agtccaccag cattatacaa aaaataataa tctgatggcc
    16501 tttgtcaccg tggaagattg ggatacttcg atagatgtgg tcgtatttcc tgatacctat
    16561 gataattata aaaatgcttt acaacagggt aaggtcataa tgatcgctgg caataggtct
    16621 gatagtgata gctttatagc caataagata atagatttaa atctgaatat gctggagatt
    16681 aatttgatca accttgaaat tgatcaatta aatgaattaa ggagatttat taattcaaat
    16741 aaaggccaaa acccggttat ctttaataat agtactaaga aagtgataac cgataaagag
    16801 tactggattg atttaagtga tgctacaatt aaacaattag atgatataat ttcaggccag
    16861 aattataaga tttattaaaa atatagtaga atagtatttg aaataataat ttagatatga
    16921 tgaagtaata atttggaggc aataagatga acagattttg ggaactattt ataaaaggaa
    16981 tgccaattgg gatgtctaat acattaccag gtattagcgg tggtactatt gccctggtgc
    17041 taaaaatata tgatgaatta gtacatagta taaaaaaaat aaaaattaaa tttttaattc
    17101 ccgttatttt aggagctgtg gctggtgtat ttatcggttc cagtataata ataaccttat
    17161 ttaacaatta tccgatattt gttactgcct ttttattagg tctgatttta gcatcttcta
    17221 aagtaacttt tcaagaaatt gatactttta atattctaaa agtaatgttt ggaattgctg
    17281 gatttattat agcatttatg tattcagctg aagcaagtaa tacggtaaat attaacaata
    17341 tctcccttat taagtacttt gccgggggtg ccttaggcag tgtggctatg attttacccg
    17401 ggataagtgg agggacgatc ctggttatga tgggactata ccatagtgta ctggctgcca
    17461 ttagtggtct taaccttgtt atcataacag tatttgggct gggtatctta tcaggtctct
    17521 taattttctc ctggctttta tcctatttat tagataatta tcgctcatct ttaatggcac
    17581 ttttaaccgg ccttatatta ggttcgacca gaagtgttat tcccgctcaa tttaatatta
    17641 ttgcagtttt aggtttaata ttaggtatac tggttatcta ttatcttcta atgatagaat
    17701 aagaagttaa attcctgtaa acatttattt agactagtta attttaatca ttgtcaagcc
    17761 tgttataaca gtatacaata aaaaaataaa catatgttta ccacctacat gtaatttctt
    17821 gacattgtca taaaaagatt atattctggt aatatgtatt tatagaataa ttataaactg
    17881 taaggaggca ttactgtgaa aaagattggt attctgacta gtggtggaga tgctcctggt
    17941 atgaatccgg ctataagatc ag
//
LOCUS       DATGCJ010000045         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_542433, whole genome shotgun sequence.
ACCESSION   DATGCJ010000045 DATGCJ010000000
VERSION     DATGCJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542433"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VKN64_02160"
     CDS             <1..1159
                     /locus_tag="VKN64_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA59068.1"
                     /translation="KMIYFNNAATTYPKPESVYQDVDSFFRNEGVNPGRSQGVKTMNI
                     NRRIFNTREKIAQFFGIKDSSRFIFTSGATESLNLAIKGFLKKGDHVISTKLEHNSVI
                     RPLNKLQKEGRITVTFIDFNKKGFIEIESLKQAVKDDTSLIILSHASNVLGTVNDIAE
                     ISHFAKENNIKILIDAAQTAGVLDINLNNLEIDMMSVPGHKSLFGPPGIGGLYISENI
                     ELDTLMEGGTGSNSLNTYQPELMPDKFESGTLNTLGIIGLGAGIDFINKTGQAEIYQH
                     ELNLLDKFIEGLRNIKGILMYGTTNLDKKVGVVSINLMDLSANRFAHLLQNEYNISVR
                     GGLHCAPLLHKAMQTDQKGMVRFSFSYFNTLQETNEALNVIETIAKKEGSR"
     gene            1156..2559
                     /gene="selA"
                     /locus_tag="VKN64_02165"
     CDS             1156..2559
                     /gene="selA"
                     /locus_tag="VKN64_02165"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HMA59069.1"
                     /translation="MKKELFKQIPAVNDILESKQGESLIEKYSHNLVLKAVRSVLDEK
                     RNTIAKTPEPELENMHLNLKFASLFNEIEDYIEDYLAPNLENVINATGTIVHTNLGRS
                     LLSKEAVDSLVNVAENYSTLEIDKQSGERGSRYNNVREFLIELSGAEDGLVVNNNAAA
                     VLLILDTFAKNKEVILARGEMVEVGGSFRIPEVMEKSGAELIEVGATNKVYKKDYEDK
                     INEETGMLLKVHTSNYKVMGFTKEVQLKELVELGKEYNLPVVEDLGSGILRDLSDLGL
                     LDEPTVADKIAQGADLVSFSGDKLLGGPQAGIILGKKDYISKLKKNPLTRALRVDKFT
                     LASLEATLKAYIKEEDREVLPTLKMISEDIEVIKNKAKRLRDKLYNINVDRINTDIRK
                     DSSKTGGGAFPVQEIPTYVTRVQIKDMQLNDLSYKLRMNNPPIFTRLKDDYMLFDLRT
                     IKDEEIEEIVTAFKKII"
     gene            2577..4481
                     /gene="selB"
                     /locus_tag="VKN64_02170"
     CDS             2577..4481
                     /gene="selB"
                     /locus_tag="VKN64_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HMA59070.1"
                     /translation="MSQKNFIIGTAGHIDHGKTTLIKALSGTDTDRLSEEKERGISID
                     LGFTDLKLNDQYTLGIVDVPGHEKFIKNMLAGAGGVDIALLVIAADEGVMPQTREHLD
                     ILSLLNVQKGLIAVTKIDMVEDEWVELMVEEIREKLSDTFLSDSPIIPVSATEGIGLE
                     KLKNQLIKTIESMETKEITGNVYYPIDRVFTIKGHGTITTGTLVSGKINVDDNLLIYP
                     SHKEVRVRSIEVHGKDSEAAYPGQRVGINLSGIDKDEIERGDVLATPDSLINTKFLDG
                     RLTVLKNAPLVVEHGDRIRFHIGAKEVIGRLYLLDKKELLPGERGLVQYRLEEEVVAR
                     YNENYVIRRYSPMTTIGGGNIIDTHPHLRKRFKEDVIEELNKKEELADKERVEYICKL
                     AQAETVTYDYLIKKTSIRKDKIKEILQELNEEDKVKYFQRGNEKTWIHVDNYQELAAE
                     LLEILEKFHGKNRLKAGLSKEELRTRLSSELDNKEYNQFLNGLEKREKIEVKGSYVKK
                     FAYQVNLNEKEKKLRDEILKEFKENKFMPPNVEDIIEPNSKKEEVFEYLIDNGKIVHL
                     REDLFFLSSAVKEAKELLINHLEEKDTITLAEFRDKLDSSRKYTLALLEYFDQQNITK
                     RVGEERTLTK"
     gene            4532..4630
                     /locus_tag="VKN64_02175"
     tRNA            4532..4630
                     /locus_tag="VKN64_02175"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4567..4569,aa:Sec,seq:tca)
BASE COUNT         1730 a          619 c          937 g         1349 t
ORIGIN      
        1 aaaaatgatc tattttaaca atgcagcgac cacttatccc aaaccagaat cagtatatca
       61 agacgttgac agctttttta gaaatgaagg tgtcaacccg ggaaggtcgc agggtgtaaa
      121 aacaatgaat ataaaccgga ggatatttaa tacaagggag aagattgccc aattttttgg
      181 tattaaagat tcctcacgtt tcatttttac atctggagca actgaatcct tgaatctagc
      241 aatcaaggga tttctaaaaa aaggagatca tgtaatttct actaagttag aacataactc
      301 agtaatccgc cctcttaaca agttacaaaa agagggacgg attactgtta cttttattga
      361 cttcaataaa aaaggtttta ttgaaataga gagtcttaaa caagctgtca aagatgacac
      421 ttcccttatt attctttccc atgcctccaa tgttttaggt acagttaatg atatagcaga
      481 aatttctcac tttgcaaaag aaaataatat aaagatactt atagatgctg cccagaccgc
      541 gggcgtttta gatattaatt taaataattt agagattgat atgatgtctg tacccggcca
      601 taaatcactt tttggcccac ctggaatcgg gggattatat atttctgaga atattgaact
      661 agatactcta atggaaggtg gtaccggtag taattcttta aatacttacc agcctgagct
      721 catgcccgat aaatttgaaa gtggtacact taatacttta ggtattattg gtttgggagc
      781 gggtattgat tttattaata aaactggtca agcagagatt tatcaacatg aattaaatct
      841 attagataaa tttatagagg gacttaggaa tatcaaaggt atacttatgt atggaactac
      901 taatttagat aaaaaagtag gggttgtaag tataaattta atggatctaa gtgctaatag
      961 atttgctcat ctactgcaaa atgaatataa tatatctgta aggggtggac ttcattgtgc
     1021 tcccttactt cataaagcga tgcaaaccga tcagaaaggt atggtcagat ttagttttag
     1081 ttattttaat actttacaag agaccaatga ggctttaaat gtgattgaaa ctattgccaa
     1141 aaaagagggg tcaagatgaa aaaagaactt tttaaacaga taccagctgt taatgatatt
     1201 ttggagagta aacagggaga aagtttaata gaaaagtaca gccataatct agttttaaag
     1261 gcagttcgga gtgttttaga cgaaaagagg aatactattg ctaaaactcc cgaaccagaa
     1321 ctagagaata tgcatttaaa tttaaagttt gcttctttat ttaatgaaat tgaagattat
     1381 attgaagatt atcttgcacc taatttagaa aatgttatta atgctaccgg tacaattgtt
     1441 cataccaact taggcagatc tttgttatca aaagaagccg ttgattccct ggtcaatgtg
     1501 gctgaaaatt attctacttt agagattgac aagcaatcag gtgagagagg ttcccgttat
     1561 aataacgtta gagaattttt aattgagtta agtggagctg aagatggact ggttgtaaat
     1621 aataatgcgg cggccgttct tttaatctta gatacctttg ctaaaaataa agaagtgatt
     1681 ttagcccgtg gtgaaatggt tgaagttggc ggatcttttc ggatcccgga agtaatggaa
     1741 aaaagcggag cagaattaat agaagtagga gcaaccaata aagtatataa aaaagattat
     1801 gaagataaaa taaatgaaga gacaggtatg ttactaaaag ttcataccag caactataaa
     1861 gtgatgggct ttaccaaaga agttcagtta aaagaattag ttgaattggg caaagaatat
     1921 aatttacccg tagtagaaga tctaggtagt ggtattttaa gagatttatc agatttgggt
     1981 ttattagatg aaccgacagt tgctgataaa attgctcagg gagctgatct ggtttccttt
     2041 agcggtgata agctcttagg aggccctcag gctggtatta ttttaggtaa gaaggactat
     2101 atatcaaaac taaagaaaaa ccccttaaca agggctttaa gggtcgataa atttacttta
     2161 gcatcactgg aagctacctt gaaagcctat attaaagaag aagatagaga agtactgcct
     2221 accttaaaaa tgataagtga agatattgaa gttattaaaa ataaagcaaa gagattaaga
     2281 gataaattat ataatatcaa tgttgataga attaacactg atattagaaa ggattcctct
     2341 aaaacaggtg gaggggcttt tccagtgcag gagatcccta cctatgtgac cagagtacag
     2401 ataaaagata tgcagttaaa tgatttatca tacaaattaa gaatgaataa tccacctatt
     2461 tttactagat taaaagatga ttatatgtta tttgatttaa gaactattaa ggatgaagaa
     2521 atagaagaga ttgttacagc ttttaagaaa ataatttaag ccgggaggag cttgttatgt
     2581 cccaaaaaaa cttcataatc ggtacagccg gacatattga ccacgggaaa acaactttaa
     2641 taaaagcatt aagcgggaca gatacagata gactatcaga agaaaaagaa agaggcatct
     2701 ctattgattt aggatttact gatctaaaat taaatgatca atataccttg gggatagtag
     2761 atgtacccgg tcatgaaaaa tttattaaga acatgttagc tggagcaggt ggtgtagata
     2821 tagccttatt ggtaattgca gctgatgaag gtgttatgcc ccagacccgt gaacatttag
     2881 atattttaag cttattaaac gtacaaaaag gtttaatagc ggttacaaag atagatatgg
     2941 tagaagatga atgggtcgaa ctcatggtag aagagataag agaaaagtta tcagatactt
     3001 ttttaagtga ttctcccatt attccggttt cagctaccga gggaatcggc ttagagaagt
     3061 taaaaaatca attaattaaa actattgaaa gtatggaaac aaaagaaatt actggtaatg
     3121 tatattatcc catcgacaga gtatttacta ttaaaggaca cgggacaatt acaaccggga
     3181 ccttagtaag cggtaaaatt aatgtagatg ataatctatt aatctatcct tcccataaag
     3241 aagttagagt aagaagtatc gaagtacacg gcaaagattc agaagctgct taccccggac
     3301 agagggtagg tattaatctt agtgggatag ataaagatga aatcgagcgg ggcgatgttt
     3361 tagcaacccc tgacagcctt attaatacca aatttttaga tgggcgttta actgtattaa
     3421 agaatgctcc tttagttgtt gaacatggtg atagaataag atttcatatt ggagctaaag
     3481 aggttattgg ccgcctctat cttttagata aaaaggaact tttaccgggt gaaagaggct
     3541 tagttcaata cagattggaa gaagaggttg tggcccgtta taatgaaaat tatgttatta
     3601 gaagatattc tccgatgacc actattggag gaggcaatat aattgatacc catcctcatt
     3661 taagaaaaag atttaaagaa gatgtgatcg aggagttgaa caaaaaagaa gaacttgccg
     3721 acaaagaaag agtagaatat atatgcaaat tggcccaagc tgaaactgta acttatgatt
     3781 atttaatcaa aaagaccagt attagaaaag ataaaatcaa ggaaatatta caagaactaa
     3841 atgaagaaga taaggttaaa tactttcaaa gaggtaatga aaagacctgg attcatgttg
     3901 ataattatca agaactagcc gctgaattat tagagatttt agagaagttc catggtaaaa
     3961 atagattaaa agcaggttta tcaaaagagg agctaagaac aagattatct tcagaacttg
     4021 ataataaaga atataatcaa tttttaaatg ggctagaaaa aagggaaaag attgaagtta
     4081 aaggttccta tgttaagaaa tttgcttatc aagtaaactt aaatgaaaaa gaaaaaaaat
     4141 taagagatga aatattaaag gaatttaagg aaaataaatt tatgcctccc aatgttgagg
     4201 atattataga gcccaattca aaaaaagaag aagtatttga atacctgatt gataatggca
     4261 aaatagttca tcttagagaa gacttatttt tcttatcgtc tgctgtgaaa gaagcaaaag
     4321 agttattgat taatcattta gaagaaaaag atactattac attggctgaa tttagagata
     4381 aattagacag cagtcgtaaa tatacattag cattattaga atattttgat caacaaaata
     4441 tcactaaaag agttggggaa gaaagaactt tgacaaagtg atttacaaag tacaaacttt
     4501 atagtataat agtttttgga caaaaaaata ggggagcggc tgtgtccatg gtggacaccc
     4561 cggacttcaa atccggtggg aggtcggcag accggccttt ggtgggttcg attcccacac
     4621 gttcccgcca ttgat
//
LOCUS       DATGCJ010000046         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_542481, whole genome shotgun sequence.
ACCESSION   DATGCJ010000046 DATGCJ010000000
VERSION     DATGCJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542481"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VKN64_02180"
     CDS             complement(<1..1590)
                     /locus_tag="VKN64_02180"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HMA59071.1"
                     /translation="MDFMQRYKKWLKDDYFDEETQQELENIKDDEKEIEDRFYKDLEF
                     GTGGMRSKIGAGTNRINKYTIRRATQGFANYIINYYEQEGKESGVVIAYDSRHKSPEF
                     ALEAALVLAANNIKTYLFDSLRPTPELSFAVRELNACGGIVITASHNPPEYNGYKVYD
                     CTGCQLVPDLARKVIAEINEIDNFSMVKTINKQKAKEKNLLEYIGEDIDSKYIDTMID
                     TLPKNDLVKNSGKDLNIIYTPLHGSAYKLTERLLSELGFSNLSIVEKQAVFDPDFSTV
                     ESPNPEEYSAFEMALEMAEENNDETDLIMGTDPDGDRMGAIVKDDKGNYIGLNGNQIG
                     ILMADFLLNQSEVPDNGVIVKTIVTTEMIKKIAEDHGVEVLDVLTGFKYIGELITEFE
                     KKGNKEFILGFEESYGYLIGKYARDKDAVVACGLTALIALYYKKQGKTLYQRLQEVYK
                     QYGYYKEDLVAIRREGKEGQEQINGTLKHFREKGIKEIADRKVVLWKDFKEGKEYDLL
                     KETEQEINLPKSNVLQYLLDDE"
     gene            complement(1888..2973)
                     /locus_tag="VKN64_02185"
     CDS             complement(1888..2973)
                     /locus_tag="VKN64_02185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMA59072.1"
                     /translation="MIPIANPIITDEEKELVNEVMDSGMLIMGEYVKEFEEKFANYSD
                     AKHGIATSSGTTALDVAIKALDIEEGDKVITTPFTFIASSNSILYSKADPIFADVKED
                     TFNIDPDSIRELLKEHPDTKALMIVHLYGLPCDMDEIMEIVNEHNLLLIEDSAQAHGA
                     TFKGRKVGSFGDVSAFSFYPTKNMTTSEGGIVLTDDEDVMERARLILNHGSPKKYHHD
                     ILGYNFRMTNISAAIGLVQFKKLDQFNKQRQENARRLTEGLSDLTWLQTPVIPADREH
                     VFHQYTVKVDDREEFLKYLQEKEIGYGIHYPMPAYEQKLYKDLGYKDISCPVSEKLCD
                     QVVSLPVHPALSDEDIDYIIESLRDFK"
BASE COUNT          931 a          584 c          367 g         1189 t
ORIGIN      
        1 ttcatcatct aatagatact gtaaaacatt cgatttgggt aagttgatct cttgttcagt
       61 ttcttttaat aaatcatatt ctttaccttc tttaaaatct ttccataaga ctacctttct
      121 atcagcaatc tcttttattc ctttttctct aaaatgttta agagtaccat ttatttgttc
      181 ctggccttct tttccttctc ttcggatagc taccagatcc tctttataat agccatattg
      241 cttataaact tcctgtaatc tttgataaag ggttttacct tgctttttat aatatagcgc
      301 tataagggct gttaggccgc aagcaacaac tgcgtcttta tctcttgcat atttaccaat
      361 taaatatcca taactttctt caaatcctaa aatgaattcc ttattaccct ttttttcaaa
      421 ctctgttatt agctcaccta tatatttaaa tccagttaac acatcaagta cttcaactcc
      481 atggtcctct gcaatttttt taatcatttc agttgtaact attgttttaa caattacccc
      541 attatcagga acttcagatt ggtttaataa gaaatcagcc attaggatac caatttgatt
      601 tccattaagt ccaatatagt ttcctttatc gtctttaaca atagcaccca tacgatctcc
      661 atctggatct gttcccataa ttagatcggt ctcatcatta ttttcttctg ccatctctaa
      721 agccatttca aaagcagaat attcttccgg attaggactt tcaacagtag aaaagtcagg
      781 atcaaatacc gcctgttttt caacaattga aagatttgaa aatccaagtt cagataatag
      841 tctttctgtt aatttatatg cactaccatg taatggtgtg tatattatat taagatcttt
      901 accactattt tttacaagat cattttttgg taatgtgtca atcatagtat caatatactt
      961 actatcaata tcttctccaa tatattctaa aagatttttt tcttttgctt tttgtttatt
     1021 aattgttttt accattgaaa agttatctat ttcattaatt tctgcaatta ctttacgtgc
     1081 tagatcaggt acaagctgac atccagtaca gtcatatacc ttatatccat tatattcagg
     1141 cggattatgg ctagcagtga taactattcc accacaggca tttaattctc taacagcaaa
     1201 tgatagttcc ggtgtaggtc ttagactatc aaatagatat gtttttatat tatttgcagc
     1261 aagtactaat gcagcttcaa gggcaaactc aggcgattta tgtcttgagt cataagctat
     1321 tacaactcca gactcttttc cttcttgttc atagtagttt attatataat ttgcaaaacc
     1381 ttgagttgct cttctgattg tatatttgtt aattcgattg gttcctgctc caatcttact
     1441 tctcattcca ccggtaccaa actctagatc tttatagaaa cggtcttcta tttctttttc
     1501 atcatctttt atattttcta attcctgttg tgtttcttca tcaaagtaat cgtcttttaa
     1561 ccacttttta tatctctgca taaaatccat tctataatac acctctttga ttgtataata
     1621 tatactctct taatctataa taattcaatt tagaaagtta aaatcctctt tttaaagtgt
     1681 taaagagttc ccaactaata ttgggaactc tttatgttat gacaatctaa tcagccttaa
     1741 acttgttaac atttgggaga gttagaagtt ttggctgatt aaatcccagt ataatattta
     1801 ttcaaaaaat tactcatttt ataattcacg gtaaaaatgt aaattcctgc tacatttaaa
     1861 tattattaat taccttttta aatatatcta tttaaaatct cttaaacttt ctatgatata
     1921 atcaatatcc tcatcagata atgcaggatg aacaggaagt gatactactt gatcacaaag
     1981 tttttcacta accggacaac ttatatcctt ataacctaaa tctttatata acttttgttc
     2041 atatgctggc attggataat gaataccata tccgatttct ttttcctgta agtactttaa
     2101 gaactcttca cggtcatcaa ccttaactgt atactgatgg aaaacatgtt ctctatcagc
     2161 tggaataaca ggagtttgaa gccatgttaa gtcacttaaa ccctcagtta aacgccttgc
     2221 attttcttgt ctttgtttgt taaactgatc taatttttta aactgaacta atccaattgc
     2281 tgctgatata tttgtcattc taaaattata acctaaaata tcatgatgat actttttagg
     2341 agaaccatga tttaaaataa gtctggctct ttccataaca tcctcatcat cagttaatac
     2401 aataccacct tcacttgtgg tcatgttttt agtaggataa aaactaaagg ccgatacatc
     2461 accaaaggaa ccaacctttc ttcctttaaa tgttgcacca tgcgcctgtg cactatcttc
     2521 aattaaaaga aggttatgtt catttacaat ttccataatt tcatccatat cacaaggaag
     2581 tccatataga tgaactatca tcaatgcctt tgtatcagga tgttctttta atagttctct
     2641 aattgaatct ggatctatgt taaaagtatc ttcttttaca tctgcaaata ttgggtctgc
     2701 ttttgagtaa agaattgaat tactacttgc aataaaggta aaaggcgttg ttattacctt
     2761 atctccttct tcaatatcta atgctttaat tgcaacatct aaagcagttg ttccacttga
     2821 agttgcaatc ccatgttttg catcagaata attagcaaac ttttcttcaa actctttaac
     2881 atactcaccc attatcaaca tgccactgtc cattacctca ttaactagtt ctttttcctc
     2941 atcagttatt attggattag ctattggtat catctttaaa aatctccttt caaaatatct
     3001 taatatactt tactttgaat taattttatt aatttcaatg cctcaagacc atcatggcca
     3061 gttattaaag g
//
LOCUS       DATGCJ010000047         8632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_586333, whole genome shotgun sequence.
ACCESSION   DATGCJ010000047 DATGCJ010000000
VERSION     DATGCJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586333"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..2007
                     /locus_tag="VKN64_02190"
     CDS             121..2007
                     /locus_tag="VKN64_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA59073.1"
                     /translation="MKSMGDMLLRENEFSEIVIGNTALVRAMVEAGTRVVTSYPGSPM
                     PETAEAIRSIPKEKRPFYFEFSTNEKVATEIAFGASVNGHFSTVFFKSVGLNVAADSF
                     VQLPLMKLKGGMVVVIGDDPGANSSQNEQDNRHYAHLSYMPVFEPSNPQEVYEFYKEA
                     AKVSKEKEMPVILRLSTHVCHAKQKVNFKGYTPQQFDNKPQFSSDEENYVPITSEVFP
                     MKRRALQRIEDFQKFADNSKLNEVVDNKNKEKGIITMGVPYLSLRDVMEDIDDKVDVL
                     KLGIIYPLPRQVIAGFLKDHEEVKVLEELDNVLEKEIKVIAFEEGIETKIIGKSDIED
                     WINEYTPDKVQKVMKKTWPEVFTKKDLVEFETKVENRPAQLCPGCGHRSAFYAIKKAL
                     SEEDITVADIGCHTLGFLPPYEMGEVLLSMGHSTSTGTGLSLFNEERKVVSFLGDSTM
                     FHAGLPGIVNAVFNDHNLTLVIMENGTTAMTGHQNHAGTGANFNEETEPIAIQEMLEG
                     FGVENIYRIDTYAQSKLTETMKKAVNEEGFSVVIATHPCMLKFTREARREGSYKDNKA
                     AIDQEVCRQIHECVQEFGCPSFSIDEDGKVVVNEDLCIGDGSCLQTCPIDAIGIKTNK
                     EGSK"
     gene            2007..2591
                     /locus_tag="VKN64_02195"
     CDS             2007..2591
                     /locus_tag="VKN64_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA59074.1"
                     /translation="MEKKDRLNIYMIGVGGQGIGLLSEVLIRAADYADLNVRGVDTHG
                     LAQRGGTVQSHLRVGAGIYSPLIQKGTADIVIALERTEALRGIQNYLKPAGTLIYYNT
                     SWQTLDVRLENTDEVKKEDITRAADKLEAEVIEVYKEDLTDARMQNVVLLAEVFRQDL
                     LPDIKKEDYIKALKDLMQGEKLKTNLKLFNSILA"
     gene            2701..4485
                     /locus_tag="VKN64_02200"
     CDS             2701..4485
                     /locus_tag="VKN64_02200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HMA59075.1"
                     /translation="MERNIELKDKINNLNKKVRELNKSFERIINFSNQLNEISIENEK
                     LFWTKLLNTAMNLIKKADYGSVYKYEDGNVIFLDTVGHDLDLLRQLEIDESVFQLSDR
                     KNQVTIISEVINITKESTDEDTYSKLAQASRPIQDSITFDLYFKKEAVGGITLDIDEN
                     KSVNFNKEDKKILNYLLNITEAFYRKDVEKRLKEHQAYFQQLFDESTEAIALLDNKSN
                     IIKVNKSFEEVFGYQADEIKGKNIDDLITPEEKLDEGIRYTKKNIAGQEVKLETIRKT
                     KKGKRIFVSLHSFPINLEKGQIGIYAIYNDITERKETEKKLRRQKAYFQQLFDESTEA
                     IALLNNQNRVLKINKSFKNLFGYSLEEAQGKNIDSLIVPENKKTEGVNYTKKVLNNQD
                     IETESVRETKAGKKINVSIHAFPIGLEEGQIGLYAIYNDITQRKKEEEEIKYLSFHDQ
                     MTGLYNRRFFESEMNRLDNSRLLPISIIMSDIDGLKKINDQFGHAKGDEYIKVVAEEI
                     KEAVRKEDILARVGGDEFAILLPKTNEKEVRKIEQRIQSNVSQLNDKYDYPVGISTGY
                     GVKKASNTDLEDVFKKADYSMYKMKNRC"
     gene            complement(4502..5371)
                     /locus_tag="VKN64_02205"
     CDS             complement(4502..5371)
                     /locus_tag="VKN64_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMA59076.1"
                     /translation="MKKYKIIFSFLVIFLLILLLFQSNLSQAKNPFEVEIGTSYSHSS
                     NNRLNVSGYSVVEVVNVGKLEVRKIRRDILTSNLKLRYKNEKSSLELVIPYQVQRENI
                     IKYADTTENGEPTEEIKTSHGIGDITVNLQTDLRNENTKFNLGIKTTTGKEYGGNTDL
                     RFGTNYYGIKVGVSHIKQIDPVVLFGSTNYFWNIEKNNFNPGDTFQYSLGLAYALSQN
                     LSINTRIEHSITTSTFQKDKKIIGSSLNSSSIYLGTSYVNQKESALDFLVGVGLSEDS
                     ADFSFQINKPYYF"
     gene            complement(5466..5840)
                     /locus_tag="VKN64_02210"
     CDS             complement(5466..5840)
                     /locus_tag="VKN64_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59077.1"
                     /translation="MKRKLKLSSLLLIIFMVSVIVTPISAADIPDSLSQLNDKELSGI
                     RGMGQNFSIEQINSSGFKFRYSGNKGNAFKNASGVMNITNVSGDGNTINNIIDLKINI
                     YNIEEVESLDLSDIKNFIDIKD"
     gene            complement(5880..6575)
                     /locus_tag="VKN64_02215"
     CDS             complement(5880..6575)
                     /locus_tag="VKN64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HMA59078.1"
                     /translation="MKKDYYTTVIFLMLILIVSSLSVQAGNISFQIFSSGSRITRSIK
                     SKTEIKFDNIVKQKYDVSCGSAALATIFQYYYNDETTEQEIINIIKSLKNMNTLKNSS
                     GFTLLDLKRAAKFLGYKAHGLKGSVNAIRKLNLPMIVLLNSPKGPHFVVIKSVNESSV
                     DIADPALGNLTLSIESFKSQWNNTLLAIESNNRVGNNNFSEFEKPDLPTKNAIFNLMK
                     NNWTILPTDYSEF"
     gene            complement(6659..7186)
                     /locus_tag="VKN64_02220"
     CDS             complement(6659..7186)
                     /locus_tag="VKN64_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59079.1"
                     /translation="MKKLLGIGTVLMVLLALVAVPAAAQVGPLNEEMPAEELEFDLIF
                     DEAETEYLFIGEEFYERSDVITGNAFQGAMGITTVQQAAGNTSILESNVEVNAAADLE
                     PADELELSAQEVEVEGVLLLERHYSRTDTISGNAFQNATGITTVNMSSGNANILQSNV
                     EVNIGGGLFIPEPEM"
     gene            7682..>8632
                     /locus_tag="VKN64_02225"
     CDS             7682..>8632
                     /locus_tag="VKN64_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HMA59080.1"
                     /translation="MENKIEIYTLGEFKVKYNDKIISPKEKVNSKPWLFFKYLITEKE
                     KNLHPEVISSDLWPKKKIKNPKHSITNLVYRLRKKFKLSNIKDSNKYIINSNGSCFFN
                     TESNYWLDIEEFLELSQKASRIKRNKPTQAIPLFKESLNLYNGDYLPEIPYQEWVIPH
                     RNNYHWLFVETVIEYIKLLKKYDRYREITTIAEKALNIESYEEELHINYLDALLKLGE
                     NAHARSHYTYANELFNDNLDISNLPKLQSFFETNNNSNNFITDIQKKLKDRNNIKGAF
                     TCKNDIFRMIYELEERRTERTDKPVFLSYLKFEKEEILKKN"
BASE COUNT         3167 a         1250 c         1463 g         2752 t
ORIGIN      
        1 aaaattgact attattgtat atttttaaag gaataaaagg ttgataaaag aataaataat
       61 ataagaagtt aattgctatt agagtaatta tggattaata tttcataagg gagtggttag
      121 atgaaatcaa tgggtgatat gttgttaaga gaaaacgagt tttcggaaat tgtcattggt
      181 aatacggctt tagtacgggc tatggttgaa gcgggaacaa gagtagtaac ttcatatccc
      241 ggttccccga tgcctgaaac ggccgaggca attaggagta tcccgaaaga aaagaggccc
      301 ttttactttg aattttccac caatgaaaaa gttgcaacgg aaattgcctt tggggcttct
      361 gttaatggac atttttcaac cgtgtttttt aagagtgtgg gtttaaatgt ggcggctgat
      421 agttttgtac agttacctct catgaaatta aaaggtggta tggtcgtagt aataggagat
      481 gaccccggtg ctaattcatc ccaaaatgaa caggataata ggcattatgc ccatttatcc
      541 tatatgccgg tatttgaacc atctaatcca caagaagtat atgaattcta taaagaagca
      601 gctaaagtat caaaagaaaa agagatgcct gtgatcttga gattaagtac ccatgtctgt
      661 catgctaaac aaaaggtaaa ctttaaagga tacactcccc aacaatttga taataaacct
      721 caattttcaa gtgatgaaga aaattatgta cctataactt ccgaagtttt tccaatgaag
      781 agaagggctt tacaaagaat tgaggacttt caaaaatttg cagataattc taaattaaat
      841 gaagttgttg ataataaaaa taaagaaaaa ggaataatta cgatgggggt cccttattta
      901 tccctaagag atgtaatgga agatatagat gataaggttg atgtattaaa actaggtatc
      961 atctatcccc tgcccagaca agtaatagcc ggatttttaa aagatcatga ggaagtaaaa
     1021 gtcttagaag aacttgataa tgttttggaa aaagaaatta aggtaattgc ctttgaagaa
     1081 ggcatagaga ccaagataat cggtaaatca gatatagaag attggattaa cgaatatact
     1141 cccgataaag tgcagaaagt aatgaaaaaa acctggcctg aagtattcac aaaaaaagat
     1201 ctagttgaat ttgaaacaaa agttgaaaac agaccggctc aactatgtcc cggttgtggc
     1261 catagatcag ctttctatgc aatcaaaaag gcattatcag aggaagacat cactgtagca
     1321 gatatcggtt gtcatacact aggattttta ccgccctatg aaatggggga agtattactt
     1381 tccatgggcc attcaacttc aactggtacc ggactttctc tctttaatga ggaaagaaaa
     1441 gtagtctcat ttttagggga ttctaccatg ttccacgcgg gtttaccggg cattgttaat
     1501 gcagttttca atgatcataa cttaacttta gtgatcatgg aaaatggtac aactgctatg
     1561 accggccatc aaaaccatgc cgggacaggc gctaacttca atgaagaaac agaaccaatt
     1621 gcaatacaag agatgttaga agggttcgga gtagaaaata tatataggat agatacctat
     1681 gcccaatcta aactaactga gaccatgaaa aaagccgtta atgaagaagg atttagtgtt
     1741 gtaatagcta cccatccctg tatgcttaaa ttcacgcgag aagcccggag agagggttca
     1801 tataaggata ataaagcagc cattgatcag gaagtctgta gacagatcca tgaatgtgta
     1861 caggaattcg gttgtccttc ctttagtata gatgaagatg gtaaggttgt ggtaaatgaa
     1921 gatttatgta tcggagacgg atcttgttta caaacctgcc ccattgatgc tattggcatt
     1981 aagacgaaca aggaggggag taaataatgg aaaaaaaaga taggttaaac atttatatga
     2041 tcggtgtcgg gggacaggga attggtcttt taagcgaagt tttaatcaga gcggctgatt
     2101 atgctgattt aaatgtgagg ggtgtagata cccatggttt ggcccagagg ggtggaacgg
     2161 tacaatcaca tctacgagta ggagccggta tttattcacc cttaattcaa aaaggaacag
     2221 ctgatattgt aatagcctta gaaagaactg aagctttaag aggtatacaa aattatttaa
     2281 aaccggccgg gactctaatc tattataaca cctcctggca gaccttggat gtaaggttag
     2341 aaaacacaga tgaagtaaaa aaagaagata taacaagagc agccgataaa ttagaggcag
     2401 aagtgattga ggtatacaaa gaggatctta ctgatgctcg catgcagaat gtagtactct
     2461 tagcagaagt atttagacag gatttattac cagacattaa aaaagaagat tatattaagg
     2521 ccttaaaaga tctcatgcag ggtgaaaaac taaagacaaa ccttaaattg tttaactcca
     2581 tacttgcata aaaagattgt tttaagctat taatacagtt tataatttag cacaaattct
     2641 tgaaattata tttgtttaaa tttataatat taacaacttc actaattagg agcctgcttt
     2701 atggaaagaa atattgaact aaaagacaaa ataaataatt taaataaaaa agttagggaa
     2761 ttaaataaga gttttgaaag gattataaat ttttctaatc agcttaatga aataagtata
     2821 gaaaatgaaa aattattctg gaccaaactt ttaaatacgg ccatgaattt aattaaaaag
     2881 gctgattacg gtagtgtcta taaatatgaa gacggtaatg tgatattttt agatacagtt
     2941 ggacatgacc ttgacttact tagacagctg gaaattgatg aaagtgtctt tcagctttct
     3001 gataggaaaa atcaggtgac aattattagt gaagtaatca acattactaa agaatcaaca
     3061 gatgaagata cttattctaa attggcccag gcttctagac caattcaaga ttcaattaca
     3121 tttgatttat actttaaaaa agaagcagtt ggcggtatta cccttgatat tgatgagaat
     3181 aaatcagtta actttaataa agaagataaa aagatattaa attatctttt aaatataact
     3241 gaggcttttt atagaaaaga tgtggaaaaa agattaaaag aacatcaagc ttacttccaa
     3301 cagctttttg acgaatcaac tgaagcaatt gcccttttag ataataaaag taatatcatt
     3361 aaagtcaata aaagttttga agaagtattt ggatatcaag ctgatgaaat taaaggtaaa
     3421 aatattgatg atttaataac gcctgaagaa aaattagatg aaggaataag atatactaaa
     3481 aaaaatattg ccggtcaaga ggttaaatta gaaacgatca gaaaaaccaa aaagggtaaa
     3541 agaatatttg tttctctgca ctctttccct attaatttag aaaaaggaca gattggtatt
     3601 tatgcaattt acaatgatat tacagaaaga aaagaaacag agaaaaagtt aaggcggcaa
     3661 aaggcatatt ttcaacaact ctttgatgaa tcaaccgagg caattgctct attaaataac
     3721 cagaaccggg tacttaagat aaataaaagc tttaaaaatt tatttggtta ttcattagaa
     3781 gaagcccagg gtaaaaacat tgacagtcta atagtacctg aaaataaaaa aactgaaggt
     3841 gtcaattata ctaaaaaagt cctaaataat caggatatcg aaactgagtc tgttagagaa
     3901 acaaaagcag gcaaaaaaat aaatgtttct atacatgctt ttcctatagg tttagaagaa
     3961 ggtcaaatcg gtttatatgc tatctataat gatataactc aacgcaaaaa agaggaagaa
     4021 gaaattaagt acttatcctt ccatgatcag atgacaggtc tgtataatag aagatttttt
     4081 gaaagcgaaa tgaatcggct tgataattct aggttattac caatcagtat cataatgtct
     4141 gatattgatg gattaaaaaa gatcaacgat caatttggac atgctaaagg tgatgaatat
     4201 ataaaggtag tagcggaaga aattaaagag gcagttagaa aagaagatat attggcccgg
     4261 gtcggcggtg atgaatttgc catactttta cccaaaacta atgaaaagga agtacggaaa
     4321 atagaacaac gtattcaatc aaatgttagt caattaaatg ataaatatga ttacccggtc
     4381 gggatttcta cgggttatgg tgtaaaaaaa gcttctaata cagatttaga agatgttttc
     4441 aaaaaagctg actactccat gtataaaatg aaaaatagat gttaatttaa agccatatta
     4501 tttaaaaata atatggctta tttatttgaa atgaaaagtc ggctgaatct tctgataatc
     4561 ctacccctac caaaaaatca agagccgatt ctttttgatt tacataactt gtccccaaat
     4621 atatagaaga ggaattaagg ctggaaccta ttattttttt atctttttga aaagtactag
     4681 tggtaatgga gtgttcaatt ctggtattta tagataagtt ttgacttaga gcgtatgcta
     4741 aacccaatga atattggaaa gtatcccctg ggttaaagtt gtttttttct atattccaga
     4801 aataattcgt gcttccaaat agaaccacgg gatcaatttg ttttatgtga ctaactccta
     4861 ctttgatacc ataataattg gtcccaaacc ttaaatcagt attgccccca tattccttac
     4921 ctgtggtggt tttgatacct agattaaatt ttgtattttc atttcttaaa tctgtttgta
     4981 gattaaccgt aatgtcacca ataccatgag atgttttaat ttcttcggtt ggttctccat
     5041 tttccgtggt gtcagcatat ttaattatat tctctctttg aacctggtac gggataacta
     5101 attccagact ggatttttca tttttatatc taagcttaag atttgatgtt aaaatatctc
     5161 ttctaatctt tcttacttcc agtttaccga cattcaccac ttcaactaca ctatatccac
     5221 tgacatttaa ccggttattt gaactgtgag aataagaggt gccaatttca acttcaaaag
     5281 gatttttagc ctgactgaga tttgattgaa atagtaataa aatcagtaaa aaaataacta
     5341 aaaaagagaa aataatttta tattttttca ttgccattac ctccagaata atattaatat
     5401 tcttgataca aatttcttgt aaaaaaatat cccccatctt catggaggat aatatataaa
     5461 tcaatttaat cctttatatc tataaaattt ttaatgtctg atagatcaag gctttctact
     5521 tcttcaatat tataaatgtt aattttaaga tcgattatat tatttattgt attgccatca
     5581 ccagagacat tggtaatgtt cataactcct gaggcgtttt taaaagcatt acctttattt
     5641 ccactgtatc tgaatttgaa accagaagaa tttatttgtt caatactaaa attttgtccc
     5701 atacccctta tcccagatag ttccttgtca tttaattgag ataagctgtc cggaatatct
     5761 gcagcagaaa taggagttac tattactgag accataaata ttataagaag tagactgctt
     5821 aactttaatt tccttttcat gatctccctc ctatcttttt aattaataat gttaataatt
     5881 taaaattcac tatagtcggt tggcaatatg gtccagttgt ttttcattag attaaatatt
     5941 gcatttttgg taggtaaatc gggtttttca aattcactaa aattattatt accaactcta
     6001 ttattacttt caattgctaa taaagtatta ttccactgtg atttaaaact ttcaatacta
     6061 agagttaaat ttccaagggc cgggtctgct atatcaactg aactttcatt aactgatttg
     6121 attactacaa aatgagggcc tttgggtgag tttagtaaaa caatcattgg tagattaagc
     6181 ttacgtatcg cattaacact accttttaat ccatgagctt tataacccaa aaacttagct
     6241 gccctcttta agtccaataa ggtaaatccc gaactgtttt ttaaggtgtt catattttta
     6301 aggcttttta taatattgat tatttcttgt tcagtagttt catcattata gtagtattga
     6361 aagatggttg ctaaggcagc tgagccacag ctcacatcat atttttgttt aacaatgtta
     6421 tcgaacttta tttctgtttt actcttaata cttcttgtta ttcttgagcc agaggagaat
     6481 atttgaaaac taatattgcc ggcttggact gatagactgg atactattaa tataagcatt
     6541 aaaaatatga ctgtagtata ataatctttt ttcattgtca ttatcccctc tctttttgag
     6601 attaggataa ggaataagca gggtataaaa ccctgcttat cactatattt attttatatt
     6661 acatttctgg ttctggaata aataggcctc cgccaatatt tacttcaaca tttgattgaa
     6721 ggatattggc attaccggaa gacatattaa ctgtagttat accagttgca ttttggaagg
     6781 cattaccaga aatagtatca gttctggagt aatgtctttc aagaagtagt acaccttcaa
     6841 cttcaacttc ttgtgcactt agttcaagtt catctgctgg ttctagatct gcagccgcat
     6901 taacttctac attactttct aagatactgg tattaccggc agcctgttga actgttgtaa
     6961 tacccatagc gccttggaat gcattaccgg tgattacatc acttctttca taaaattctt
     7021 ctcctataaa tagatattca gtttctgctt catcaaaaat aagatcaaat tctaattctt
     7081 ctgcgggcat ttcctcattt aatggaccaa cctgagcagc agctggaaca gctaccaatg
     7141 ccaaaagaac catcaatact gtaccaatac ctaataattt tttcattatt aaaccctcct
     7201 aagttttagt tgattaaaat atttttttga attgaaaact aaataatctc aaaaggttgg
     7261 gatctaatat tattgtttcc tcctttgtat taattatttt gttttcaatt cagcttgctt
     7321 acatcatatc agtcacatat cagaaattaa tttaaaaata atcccaaaat aatcacaaga
     7381 cctttgtttt aatatacata atatggtaat taaaagataa gatgtattta tatataaaaa
     7441 tttacataaa taatggggtg aactctcata atatagtcat agataaatca tttaaaaatt
     7501 tttaaagcaa catatgttta ctaaaaatct tgataaaaat actaaggttt gttaaaataa
     7561 atttagagtt aaaagattat aatataaaaa tagtaatgaa aataaaaaat actccattac
     7621 tttattctat atatcatgat aattaataat ttaacctcat aatataaact ggtgtgatat
     7681 tatggagaac aaaatagaga tttatacttt aggagaattt aaagttaaat ataatgataa
     7741 aataatttct ccgaaagaaa aagttaattc aaaaccatgg ttatttttca aatatttaat
     7801 aactgaaaaa gaaaaaaacc tgcatccgga agttataagc agtgatttgt ggcctaaaaa
     7861 gaaaataaaa aatccaaaac attcaattac caatcttgtt tatagattaa ggaaaaaatt
     7921 taaattatct aatattaaag atagtaataa atatattatc aactcaaatg ggagttgttt
     7981 ttttaataca gagtctaatt actggctgga tattgaagag tttttagaac tcagccaaaa
     8041 ggccagccgt attaaaagaa ataaacccac ccaggctata ccgttattta aagaatcact
     8101 taacttatac aatggagatt atttaccgga gataccttat caagaatggg taatacccca
     8161 tcgtaataat tatcattggt tatttgtaga aacagttata gaatatatta aactgcttaa
     8221 aaaatatgat agatatcgtg aaattacaac cattgcagaa aaggctttaa acatcgaatc
     8281 ttatgaagaa gagctccata taaattatct ggatgccctc ttaaaattag gagaaaatgc
     8341 tcatgcaaga tctcattata cttatgctaa tgaacttttt aatgacaatt tagatattag
     8401 taatctaccc aaactgcaaa gtttttttga aacaaataat aatagtaata attttattac
     8461 tgacattcaa aaaaaattaa aggacaggaa taatataaaa ggtgccttca cctgcaaaaa
     8521 tgatatattt aggatgattt atgagcttga agagagaaga acggaaagaa ctgataaacc
     8581 cgtattttta agttatctaa agtttgaaaa ggaagagatt ttaaagaaga at
//
LOCUS       DATGCJ010000048        15076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_363653, whole genome shotgun sequence.
ACCESSION   DATGCJ010000048 DATGCJ010000000
VERSION     DATGCJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363653"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VKN64_02230"
     CDS             <1..618
                     /locus_tag="VKN64_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HMA59081.1"
                     /translation="EDNLDIKLHKQRVLLTAALDRFGMAEAFVDRKADLVCGDLIFGL
                     GLPIRINSLKALKIVAAIIAPIVTKLPFELVYPTGSKQDKEKETDDKFKKFYDEADII
                     AGDFHYIKSHLPNRIDNKIILTNTTTSDDIANLQARGAKALITTTPNLNGRTFGTNVM
                     EALLVAIIEKDPSQISTDDYLNVLEKLEFKPNYIDFDQGKDDIDG"
     gene            611..1693
                     /locus_tag="VKN64_02235"
     CDS             611..1693
                     /locus_tag="VKN64_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMA59082.1"
                     /translation="MDKFAFILHPLGFEDFYRKFNWMKKLPEDILKKATKYAPPFKIS
                     KITGIKSKTGKKIEGYFYACPLTTDQIINLDKDFVINKIIKTVKKAEEAGVKLIGLGS
                     FTSVVGDKGITINKNSKVPVTTGNSYTVATAIEGTKLAADKMDLDLKEEVVTVVGANG
                     SIGKTVSRLIAKEVNNLLLVSRDLDKLEKLTAKIKNENENINVKFSNKLEEALQKSRI
                     FITVSGAVEALINPQDLLSGSVVCDVARPRDVARDVGKKRDDVLVIEGGIVSIPGNVD
                     FNFNFGVEGQNAYACMAETMLLTLEEKFQSFSLGPDIQIDKVREIQELAKKHGFSLAG
                     LRSYGKLLQEKTIREIKSNSKQKSTA"
     gene            1800..2306
                     /locus_tag="VKN64_02240"
     CDS             1800..2306
                     /locus_tag="VKN64_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HMA59083.1"
                     /translation="MRRKTFTFFITLAIWILFAGKFSRDILIVGIIASLVTTWLYSDL
                     LFRSSNRKRNWAFYLRKFLYILLFIPVFFYEAFVAALKVSKHVFEKEPSFSPGIVKVK
                     TTLRNITALTILANLITLTPGTLTLDFDMRDKAFFIHWIDVTTVEQAETRREVIERFE
                     KWLEVIFR"
     gene            2303..2560
                     /locus_tag="VKN64_02245"
     CDS             2303..2560
                     /locus_tag="VKN64_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HMA59084.1"
                     /translation="MIIGMAIFLVLLSIVSSYRLIKGPTITDRLIAADTIIIFFSMVI
                     MLLALFINIRMLIDIVLAYAVLLFVDVLIFAKYFEYKELYK"
     gene            2560..2904
                     /gene="mnhG"
                     /locus_tag="VKN64_02250"
     CDS             2560..2904
                     /gene="mnhG"
                     /locus_tag="VKN64_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HMA59085.1"
                     /translation="MEIRLIISYLFMGASMIFAVVTAIGLIRFPDVYTRIHASAVVLT
                     MSAVLFSVGIAVYVWEFYLSLKILLIGLIFLITNPLATHAIARASYRRQIACPEVKSV
                     DEYSEYLESESR"
     gene            2901..3158
                     /locus_tag="VKN64_02255"
     CDS             2901..3158
                     /locus_tag="VKN64_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase subunit MbhD domain-containing
                     protein"
                     /protein_id="HMA59086.1"
                     /translation="MIILKYLLLIFLIAAAYLAITFKEELISIIFLSIFSITLTTLYL
                     IHNAPDVALAEAVIGAGLNTAIFMTVISQVREGKSIFREDD"
     gene            3159..3881
                     /locus_tag="VKN64_02260"
     CDS             3159..3881
                     /locus_tag="VKN64_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HMA59087.1"
                     /translation="MSRAKHIFTLIILSLLGVTLLVNFDKLPGQFEYANSIYHHITHN
                     GVNETGSLNFVTAILYDYRAYDSLGESTIIFGAVSGIVLLLSRKTLPVSSHGLSFIVK
                     RTFGMLTPFIFLFGIYIISHGHLSPGGGFQGGVILGAISIIFSIVYGSEFDYKRYSPT
                     TKTLLETSGALLFIGLGLLGIVMGGNFLVNLKILNSGVGSLISGGSIPFINIGIGFKI
                     GAGLAIIFYSMIQKTFKEEVNL"
     gene            3878..4216
                     /locus_tag="VKN64_02265"
     CDS             3878..4216
                     /locus_tag="VKN64_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HMA59088.1"
                     /translation="MINNLVFVIVFLLGFYAILVKKNLIKMVIGLNIMEGAIFYWFIS
                     ITDRGGMIPIVNGTQVASQMNDPVPQALILTGIVIGASTSAFLLTLIIELNKFTNSTN
                     PDQIGGMIED"
     gene            4213..5730
                     /locus_tag="VKN64_02270"
     CDS             4213..5730
                     /locus_tag="VKN64_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA59089.1"
                     /translation="MSIALILLIIVPLATAILIPFVDVFSNTARKLLVGFSTLVEFSL
                     GMFIIVSNFRKIMDNTFYLTYYLGEWIPPIGITLSMDMLSLFFSTLISFSLLFLVIYS
                     IGFIGHHEGKYYVLLFLIWAAMHGIVLTGDIFNLDVFLELLLITSAPLVAFKRNRNGT
                     EAAIKYMFYGTIGGLFIFIGVVLVYYNLGTLNFADISQNFNTLSLFVRKIIASFFLIG
                     IFIKMGIFPFHFWLSKAHSACPSSISALLSGVIVKVYVYVFIRLFWNVIGFDVLVETG
                     LDHIILVVALISSIIGHIMALYENDIKRTLAYSTIANLGMIIAVLTLNTKLALLAGLL
                     HVLAHLLMKVSLFTATGYILQFTPGHKREDLKGVAYKNMLVFVGFIIVAGGMIGLPPM
                     IGFASKWYVLKAFMTDGLSLAAVIIIIGSIFAFVYYIKYIITGFKKMTLGPPDKFRLI
                     LTVFYRERVVTDIALFFAIAVLISGIFFKVVFGPIEGAVEVIQNPSFYIDYILSG"
     gene            5760..7277
                     /locus_tag="VKN64_02275"
     CDS             5760..7277
                     /locus_tag="VKN64_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA59090.1"
                     /translation="MDVFILLLIIIPLITAFLIPLIDVFNKEIRKFLVLLAAFLEVSI
                     FLIFTIDNFEKIMDGNLLVNYHLGGWLPPLGITITMDMLAFYFSALISFSVMFAIIYS
                     IGYIGHHEGKYYVLIFILWSSMQGIVLTGDMFNFYVFIELILVSSSALIAFNRNRRAT
                     EAAIKYMIYGIIGGLFIFVGVLLLYLNLGTLNFADVHNKLAEVSYQTKSFIAVFFLIG
                     FSIKLGIFPFHFWVAEAQSAAPSPISALLSGIVEKIYIYAFIRMFWFVFGFDILQQMG
                     INNLIIYLALFSSMIGHLLALYVNNIKKTLAYSTIGHLGIIIAVLTLNTKLALLAGLL
                     HVLGHLLMKISLFTATGYILQFTPGSKREDLKGVAYKNMLIYIGFTIVAGGMIGLPPM
                     IGFASKWYMLKAFLSEGFHLGAIILTIGSIFAFVYYIKYIISGFKKMSLGPPEKFRLI
                     LTVFYRERVVTDVALFFIIAVLVSGIFFKVVFGPIEGVVNVIQNPSLYIQSIFGV"
     gene            7504..8817
                     /locus_tag="VKN64_02280"
     CDS             7504..8817
                     /locus_tag="VKN64_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMA59091.1"
                     /translation="MFYLEYNLGGWLPPLGILIKVDMLSLYFLVLTNILMFLLIFYSI
                     GYIGHHEGKYYVLLFIIWGAMQGILVTGDIFNFYVMLELVLITSAPLIAFKRNREGTK
                     AAIKYMFYGIVGGLFIFLAIILIYYNLGTLSYNEISASFKTMPIYFQNMIAIFFLIGI
                     FIKIGIFPLHFWVAKIQSASPSPISALVSGVLINIYIYDYIRIFWDVFGFEVLSNMNL
                     DYPIIFISILSSLIGHVLALNETDIKKMLAYSTIGHLGIIIATLTLNTRISLIAGLIY
                     VMSHSLMKISLFTTSGYLIQYTHTNHLEDFKGVAYRNKGVFIGFIVAAAGMVGFPPMI
                     GFVGRWYTIKAFFNSGYILPPLLIILGSLFAVVYYLRFIIKGFRKIHLEELGEFRLIL
                     SVLYRERLVVDIALIFVTISIISGLFSVFLYNPINNIVDIILLNI"
     gene            8836..10314
                     /locus_tag="VKN64_02285"
     CDS             8836..10314
                     /locus_tag="VKN64_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HMA59092.1"
                     /translation="MLELFIEHFDFTKLNLLFMSTGIIITAVALISSHDYTYEHRDWF
                     YISIFIYTISFCMLILTNNWIIFMIGWELVTITTFAMLLWGGNKIARIYLIIQFIGSN
                     FLLYIVLLIMNAGYSTIGPIDNFNLQLLLIIGVGVKSAIIGLHFWLPIVHSRAPSPLS
                     AILSGWVVKLGFILLLKTVVTGNLALFYIGIAMVIYAGIQAILATDYKVMLAFSTISQ
                     LGFIAIGIGSGNIYAYWGSVLHILAHGFAKTALFLGSGYWIKKIKSRTIYDFTNAWQK
                     DKINSVLTIFGLAVLAGVPLLAGYNSKTLIKYAAGKSIFLKYLFHLLSIITYIYVFRF
                     LRWSIFNFDKKEEINKDNIYNEGKTIFSITLPIVLILVIGFLSNSIIEIDYTDYHLFD
                     GLIKNIVYILISLGFLKMTNWVYVKEKPVPSMDLLLIRLKNWYHSFMGQEKVETLEVD
                     MENQIYNTLIHISEFVDKILPKKIQSQLLLIPIVVLLLLLFV"
     gene            10451..10933
                     /gene="greA"
                     /locus_tag="VKN64_02290"
     CDS             10451..10933
                     /gene="greA"
                     /locus_tag="VKN64_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMA59093.1"
                     /translation="MAEEVLLTEKGYQKLEQELERLTTEKRREVAKRIKVAREFGDIS
                     ENSEYDDAKNEQAFVEGRIKEIKQLIDNAKVIKEDEVSDDKVNLGTTVRLHNLDEDSY
                     VTYKLVGTAEADPLDHKISNESPIGKAILGKTVGSKLKVKVPKGTISYEIISIEKSSE
                     "
     gene            10948..12429
                     /gene="lysS"
                     /locus_tag="VKN64_02295"
     CDS             10948..12429
                     /gene="lysS"
                     /locus_tag="VKN64_02295"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMA59094.1"
                     /translation="MSEEFEKDLNKLMRQKIEKMEDFRERGIKTFDEKYEVDTYAEEI
                     KENFEEFEGEAVEMAGRLMALRTHGKASFADIQDMSGKMQLYVKVDLVGEDRYELFTE
                     LDIGDIIGFTGEVFKTNRGQISVKVKSFKLLTKSIRPLPEKFHGLKDKEMRYRQRYLD
                     LIMNPEVKDTFEIRSKIIRAFRDILEEKDFLEVETPMMHPIPGGANAKPFVTHHNALD
                     MELYLRIAPELYLKRLIVGGFDRVYEINRNFRNEGMSYKHNPEFTTLELYQAQADYHN
                     MMDITEELVVESAKQTLETTEITYQEQDIDLSVPWQRVTMKELVKKRTGFDYDELADE
                     EAAYKKAEELGVKIEEKGLSKGEVLYEVFETRVEEDLIQPTFVMDYPIEVSPLAKKKE
                     DNPNFTYRFEAFINGWEIANAFSELNNPFEQEKRFEHQMEQREAGDEEAQMMDRDFIR
                     ALEYGMPPTGGLGIGLDRLIMLMTDSKSIRDVILFPTMRPEQQ"
     gene            complement(12488..13024)
                     /gene="thiT"
                     /locus_tag="VKN64_02300"
     CDS             complement(12488..13024)
                     /gene="thiT"
                     /locus_tag="VKN64_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HMA59095.1"
                     /translation="MKISLRAITEIAVAVALAVIFNFLPLWKMPQGGSVSLEMLPILI
                     IALRWGFKEGLLAGFVYGLLQLAFNPYIIHPAQLILDYPLPYMMLGAAGIFKEQVKKF
                     KDKKINIYIILALLLGGFLRFVTHVLSGVIFFSQYAPEGANVWIYSIIYNGSYLIPSL
                     IVCYVVIAVLKQKILYIE"
     regulatory      complement(13054..13157)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13250..14383
                     /locus_tag="VKN64_02305"
     CDS             13250..14383
                     /locus_tag="VKN64_02305"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HMA59096.1"
                     /translation="MKILVAPDSFKGSLTALEVAESIGKGIKNYESNIKVDLLPLADG
                     GEGTVQSLVDATQGEIIKEKVTGPLGEEIKTNYGILGDGKTAVIEMAAASGLPLVPEE
                     KRDPTKTTTYGTGELIKSALEHGVEEIIIGLGGSATTDAGVGMAQALGVSFLDEKGEE
                     VGFGGRYLDKINEIRMDNIDNRVKDTKIRVACDVDNPLFGKDGAAYVYSPQKGADQEM
                     VKVLDKKLRKFNEKVKERLNKDVNKIAGAGAAGGLGAGLVAFLNAELETGIDIILDTV
                     EFEKRLKNVDLVITGEGRLDGQSIYGKTPIGVAKRAKNHNIPVIAIAGSLGPGAKKVL
                     DKGIDAYFSIIDKPDNLKAVIKRSNELLESTAEQIIRIFLINY"
     gene            14467..14865
                     /locus_tag="VKN64_02310"
     CDS             14467..14865
                     /locus_tag="VKN64_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HMA59097.1"
                     /translation="MFKRLEVKSSRRNDMVDITNQVLNFIKESNVQDGILIINVLHTT
                     AAVTINENADPDVKKDIINEFAKFVPKHDNYKHIEGNADAHIKTSMMGNNETIIIKNG
                     RLKLGSWQGIFLCDFDGPRTRTVILKIMED"
BASE COUNT         4958 a         2091 c         2939 g         5088 t
ORIGIN      
        1 gaagataact tagatataaa attacataag caaagagtat tgttaactgc agctttggat
       61 agatttggta tggcggaagc atttgttgac agaaaagcag atttagtatg tggtgactta
      121 atatttggac tcgggcttcc gattagaata aattccctaa aagccttaaa aattgtagcc
      181 gctataattg ctccgatagt tactaaactt ccttttgaat tagtttatcc aacagggagt
      241 aagcaggaca aagaaaaaga aacagacgat aaattcaaaa agttttatga tgaggctgat
      301 attattgcgg gtgattttca ttatataaaa tctcatttgc ccaataggat tgataataaa
      361 attatattaa ctaatactac gaccagtgat gatatagcta atttacaggc aagaggagca
      421 aaggcattaa ttactacgac acctaattta aatgggagaa catttggtac aaatgtaatg
      481 gaggctctat tagtagcgat catagaaaaa gatccttccc aaatttctac tgatgattat
      541 ttaaatgttt tagaaaaatt agaatttaaa ccaaactata ttgatttcga tcagggaaag
      601 gatgatattg atggataaat ttgcatttat tttacacccc ctcggatttg aagattttta
      661 taggaaattt aactggatga aaaaactgcc tgaagatata ttaaaaaaag caactaagta
      721 tgctcctccc ttcaaaattt ctaaaataac tggcataaaa tcaaagaccg ggaaaaagat
      781 agaaggttac ttttatgcct gtcctttaac cacggatcag attattaatc tggataagga
      841 ctttgttatc aacaaaataa ttaagacagt aaaaaaagca gaagaagctg gtgtgaaatt
      901 aattggattg ggttctttta cttctgtggt gggcgataaa gggataacga taaataaaaa
      961 ttcaaaagtg ccggttacta ccggaaatag ttatacggtg gctacagcaa ttgaaggtac
     1021 taaattagct gctgataaaa tggatttaga tttaaaagaa gaagttgtga ccgtagtagg
     1081 tgcaaatggt tccattggta aaacagttag tagattaatt gccaaagaag taaacaactt
     1141 gcttttagta agtagagatt tagataaatt agaaaaatta acagctaaaa taaaaaatga
     1201 aaatgaaaat attaatgtaa aattttctaa caaattagaa gaagccttac aaaaatccag
     1261 gatatttatt acggtttcgg gggctgttga agccctaatt aacccccagg atctactgag
     1321 cggttctgtg gtctgtgatg tagcaaggcc gcgggatgtt gcccgcgatg taggcaaaaa
     1381 acgtgatgat gttttggtta ttgaaggtgg aatagttagt atcccgggta atgtagattt
     1441 caatttcaat ttcggagtag aaggccaaaa tgcctatgcc tgtatggccg aaacgatgct
     1501 gctgacttta gaagaaaaat ttcaaagttt tagtttagga ccggatattc aaattgataa
     1561 agtaagggag atacaagagt tagctaaaaa acacggattc agcctggctg gattgagaag
     1621 ttatggaaaa cttttacagg aaaaaacaat aagagaaata aaatcaaata gcaagcaaaa
     1681 gagtaccgct taaatggtgg tacttttttt atacaaattt taataaaggg atttaacatc
     1741 agaatgttta atgttataat taagaagaat attttcaatt aaagcagagg agggaagtta
     1801 tgcgcagaaa aacttttaca ttttttataa ctttagcaat ttggattctc tttgctggca
     1861 agttttcccg tgatatttta atagttggaa ttattgcttc ccttgtaact acctggttgt
     1921 atagtgatct gttatttagg agttctaaca gaaaacgaaa ctgggctttt tatttaagga
     1981 aatttttata tattttatta tttattcccg tttttttcta tgaagccttt gtggcagcct
     2041 taaaagtgtc caaacacgta tttgaaaaag aaccctcttt ttcgcccggt attgttaagg
     2101 tcaaaacaac tttgagaaat attacggctt taactatatt agccaattta attactttaa
     2161 ctcctggtac tttaacatta gattttgaca tgcgtgataa ggcatttttt attcattgga
     2221 tcgatgtcac aacagtagaa caggctgaaa cccgccggga agttattgag agatttgaaa
     2281 aatggttaga ggtgatattc agatgattat tggtatggca atattcttag ttttattatc
     2341 tattgtatct tcatatagat taataaaagg gcctactatt acagaccgat tgattgctgc
     2401 tgatacaata ataatatttt tttcaatggt aataatgtta ttagctttat ttattaatat
     2461 tagaatgttg atagatatag tcttagcata tgcagtatta ttatttgtgg atgttttgat
     2521 atttgctaag tactttgagt ataaggagtt gtataaataa tggaaataag attaataatt
     2581 tcttatttgt ttatgggtgc ttccatgatt tttgctgtgg taacagctat tggtttaata
     2641 aggtttcctg atgtttatac acgaattcat gccagtgctg ttgttttaac aatgagtgct
     2701 gtgttatttt cggttgggat agcagtatat gtatgggagt tttacttaag cctgaaaatt
     2761 ttacttatcg gcttaatatt tttaattact aatccacttg ccacacatgc tattgcgcgg
     2821 gcttcttacc gcagacagat tgcctgtccg gaagtaaaaa gtgtggatga gtactctgaa
     2881 tatttagaga gtgagtcccg atgattatct taaaatatct tttattgata ttcttaatag
     2941 cggcggctta cctggcaatt acttttaagg aagaactaat ttcaataata tttctgtcta
     3001 ttttttctat tactttaaca actttgtatt taattcataa cgcacctgat gtggcattag
     3061 ctgaagccgt aatcggggcc ggcctaaata cagctatttt tatgactgta atcagtcagg
     3121 taagagaagg gaaaagtatc tttagagagg atgattagat gtctagggca aaacatatct
     3181 ttactttaat aattctatcc ttattagggg ttactttatt agtgaacttt gataaattac
     3241 ccgggcaatt tgaatatgca aacagcatct atcatcatat tactcataat ggggttaacg
     3301 agactggttc tctgaatttt gtgacagcaa tcttatatga ttatagagct tatgattcat
     3361 taggagaaag tactatcata tttggggcgg tgagtggtat tgtcctttta ctttctcgga
     3421 agacattacc tgtttcttct cacggtttgt cttttattgt taaaagaacc tttggaatgt
     3481 tgaccccttt catattttta tttggtatct atataatttc tcatggtcac ctttcaccgg
     3541 gaggaggctt ccaaggggga gtaatcctgg gtgcaatttc tattatcttc agtatagtat
     3601 atggtagtga atttgattat aagaggtatt ctccgactac taaaacattg ctggaaacca
     3661 gtggagcctt gctttttata ggtctgggtc tattaggtat tgttatggga ggaaattttt
     3721 tagttaactt aaagatcttg aattcagggg ttggttcttt aattagtgga ggtagtatac
     3781 catttatcaa catcggaatc ggatttaaaa ttggagccgg acttgctatc atcttttaca
     3841 gtatgattca aaagaccttt aaagaggagg tgaacctgtg ataaataatc ttgtctttgt
     3901 tatcgtattc cttttgggtt tttatgctat tttagttaag aaaaatttga ttaaaatggt
     3961 aattggttta aatattatgg aaggggcgat cttttattgg tttatttcaa taactgatcg
     4021 cggtggtatg ataccgattg taaatggaac acaggtcgcc tctcaaatga atgatcccgt
     4081 tccccaggct ttaattttaa caggaatagt tattggtgcc agtacttctg catttttact
     4141 tactttaata atcgagttaa ataaatttac taatagtact aatcctgacc agattggggg
     4201 tatgatagaa gattgagtat agccttaatt ttattaatta tcgtacctct agcaaccgca
     4261 atcttaatac cctttgtaga tgtattcagt aatacggcca ggaaattact ggttggtttt
     4321 tctactctcg ttgagttttc tcttgggatg tttataattg taagcaattt taggaaaatt
     4381 atggataaca ccttttatct aacatactat ctaggagagt ggataccgcc aattgggatc
     4441 accttaagca tggatatgtt atccctgttt ttttctactt tgatttcttt ttcactgcta
     4501 tttttagtta tctattctat tggttttatt ggacatcatg agggtaaata ttatgtttta
     4561 ttatttctaa tctgggcagc aatgcatggt attgtcttga ccggtgatat atttaacctg
     4621 gatgtctttt tagagttatt gttgataaca tcagctcctt tagtagcctt taaaaggaat
     4681 cggaatggta cagaagctgc cattaaatat atgttttatg ggactatcgg aggactattt
     4741 atctttatcg gggtagtcct ggtttattat aatctgggca ccttaaattt tgccgatata
     4801 tctcagaact ttaacaccct gtcattattt gtgagaaaga ttattgcctc attctttttg
     4861 ataggcattt ttattaaaat gggtattttc ccgttccatt tttggttatc aaaggcccat
     4921 agtgcctgtc ccagttcaat tagtgccctg ctgtcagggg taatagtaaa ggtttatgtt
     4981 tatgttttca taagattgtt ctggaacgta attggatttg atgttcttgt agaaaccggc
     5041 ttagatcata tcattttagt agttgccttg atttccagta ttattggtca tatcatggcc
     5101 ttatatgaaa atgatataaa acgaacgtta gcttattcta ctattgcgaa cctgggtatg
     5161 attattgcgg tgttaacctt aaataccaaa ttagctttat tagccggcct tttgcatgtt
     5221 ttagcccatc tattaatgaa ggtttccttg tttaccgcta ccggctatat actgcagttt
     5281 acacccggcc ataaaagaga ggaccttaaa ggtgttgctt ataagaatat gttagtgttt
     5341 gttggcttta taatagtggc tggtggaatg atcgggctgc cgccgatgat aggttttgcc
     5401 agcaaatggt atgtattaaa agcatttatg actgatggac ttagtttagc agccgttatt
     5461 ataataattg gcagtatttt tgcctttgtt tactatatca aatatattat taccggtttt
     5521 aaaaagatga ctttaggacc gcctgataag ttccgtctta ttttaacagt attttatcgg
     5581 gaaagagttg ttacggatat cgcattattt tttgcaattg cagtattaat ttctggtata
     5641 ttcttcaaag ttgttttcgg tcctatcgaa ggggcggtgg aagtaattca aaatccttcc
     5701 ttttatattg actatatact aagtggttaa ttaatcttta caactagggg gttaaaaagt
     5761 tggacgtatt tatcctatta ttaattatta ttcctttaat tacggccttt ttaatcccat
     5821 tgattgatgt atttaacaaa gaaatcagaa agtttctagt tttgctggct gcctttttag
     5881 aggtttcaat atttttaata tttactattg ataattttga aaagatcatg gatggtaatc
     5941 ttttagtaaa ttaccatctc ggtggttggc tgcccccatt aggcataact attactatgg
     6001 atatgttagc attttatttt tcagctttaa tttcattttc agttatgttt gcaataatat
     6061 attctatagg atatataggc catcatgaag ggaaatacta tgtattaata tttatattat
     6121 ggtcgtctat gcaggggata gttttaactg gtgatatgtt caatttttat gtatttattg
     6181 aacttatctt agttagttct tcagctttga tagcttttaa tcgtaacagg agagctacgg
     6241 aagctgccat taagtatatg atttacggta ttatcggtgg attatttata tttgtgggag
     6301 ttttactctt atatttaaat ttagggactt taaattttgc ggatgttcat aataaattag
     6361 ctgaggtgtc ctatcaaacc aagagtttta ttgccgtatt tttcttgata ggattttcaa
     6421 ttaagttagg gatatttccg tttcatttct gggttgccga agcacaaagt gcggccccca
     6481 gtccgatcag tgccctgctg tcaggtattg tggaaaaaat atatatttat gcttttataa
     6541 gaatgttttg gtttgttttc ggttttgata tattacaaca gatgggtatc aataatttaa
     6601 taatctatct agccttgttt tcaagtatga tcggccattt attagcatta tatgttaata
     6661 atataaaaaa gacgctcgct tactctacga ttgggcattt aggaatcata atagctgttt
     6721 taactttgaa tactaaatta gcgttattag ccgggctctt acatgtttta ggccatcttt
     6781 taatgaagat ttctctgttt acagctaccg gctatatact gcagtttaca ccaggtagta
     6841 aaagagagga tctcaaaggt gttgcttata agaacatgtt aatatatatt ggctttacaa
     6901 ttgtagcggg cggaatgatc ggtttaccgc cgatgatagg ttttgccagc aaatggtata
     6961 tgttaaaagc ctttttaagt gagggtttcc atttgggagc tattatatta acaataggca
     7021 gtatctttgc ctttgtttat tatattaaat atatcattag cgggtttaaa aagatgagtt
     7081 tagggccacc tgaaaagttc cgcttaattt taactgtgtt ttacagggaa agagttgtaa
     7141 cagatgtggc attgtttttt ataatcgcgg tcttagtttc aggtatattc tttaaggtcg
     7201 tttttggacc gattgagggt gtggttaatg taattcaaaa tccttcgtta tatattcaat
     7261 ctatatttgg tgtttagttt gattatttaa agttttgata actgaaagat taggtaagtg
     7321 ataaaggtga ataatatact aattacaata atcataatcc ctatattgac tgcattttta
     7381 attcccctta ttgataaatg gggaaaagaa aaaaggaaaa tattggttat aagctcaaat
     7441 ataatcgaaa tatttttatt tgctcgtttt atttggataa atatcgagac gataaaagag
     7501 ggaatgtttt atcttgaata taatttagga ggctggttgc cgcctttagg tattttaatt
     7561 aaggtagata tgctctcctt atacttttta gttctaacta atattttaat gtttttatta
     7621 attttttatt cgattggata tataggccat cacgaaggta aatattatgt tttattattt
     7681 atcatctggg gagctatgca ggggatatta gttactggtg atatattcaa cttttatgtt
     7741 atgcttgaac ttgtcttaat tacttcagcc ccgcttattg cctttaaaag gaatagggaa
     7801 ggaacaaaag cagccataaa atatatgttt tatggcatag tcggcggctt atttatattc
     7861 ttagctatta ttttaattta ttataatttg ggcacattaa gttataatga gatatcagcc
     7921 agttttaaga caatgccaat ttattttcaa aatatgatag ccatcttctt tttgatcgga
     7981 atttttatca agataggtat attcccctta catttttggg ttgctaaaat acagagcgcc
     8041 agtcccagtc caattagtgc cctggtttca ggggtattga taaacattta catttatgat
     8101 tatataagga tcttttggga tgtatttggg tttgaggttt taagtaatat gaatttagac
     8161 tatcccatca tctttatttc gattttatca agtttaattg gacatgtatt agctttaaat
     8221 gagactgata ttaaaaaaat gttagcatat tctacaattg gacacctggg aataataata
     8281 gctactctaa ctcttaatac caggatatcc ttaatagctg gtttaatata tgtgatgagt
     8341 cattctttaa tgaaaatctc actgtttact acaagtggat acttgattca gtatactcac
     8401 actaatcatc tggaagattt taaaggggtg gcttatagaa ataagggtgt ttttattggt
     8461 tttattgtag cagcagccgg tatggtagga tttccaccga tgataggttt tgttggtaga
     8521 tggtatacaa ttaaagcgtt ttttaattcc ggttatattt taccaccttt gcttataata
     8581 ttaggtagtt tgtttgcagt tgtgtattat ctccgtttta taataaaagg tttcagaaag
     8641 attcatttag aagagttggg agagtttaga ttaatacttt ctgttttgta tagggaaagg
     8701 ttagtggttg atatagcatt aatattcgta acaatttcaa taatttccgg cctattttct
     8761 gtctttttat ataatccgat taataatata gttgatatta tattactaaa tatataattt
     8821 tgggaggtta ttaacttgct tgagcttttt atagaacatt ttgattttac aaaattaaac
     8881 cttctattta tgagcaccgg tataataata acagcagttg cattgatttc ttcacatgat
     8941 tatacctatg aacatagaga ttggttttat ataagtatct ttatctatac cattagtttc
     9001 tgcatgctga tcttaaccaa caactggatt atatttatga taggatggga attagtgaca
     9061 ataactactt ttgctatgtt actttggggt ggtaataaaa tagcccggat atatctaata
     9121 attcagttta tcggtagtaa ctttttacta tatattgtac ttttaattat gaacgcaggc
     9181 tacagcacca ttggccccat tgataacttc aatctccagc tacttttaat tattggggtg
     9241 ggagtaaaaa gtgctataat cggtcttcac ttctggttac cgattgttca ctcccgggct
     9301 ccttcaccgc ttagtgctat cctttcaggt tgggtagtta aactgggctt catcctgctt
     9361 ttaaaaacag tggtaacagg aaatcttgcc ttgttttaca ttggtatagc tatggtgatc
     9421 tatgcgggca tccaggctat tttagcgact gattataaag tgatgttagc ttttagtact
     9481 atcagtcaac taggctttat tgcgattggt atcggtagtg gtaatattta tgcctattgg
     9541 gggagtgttt tacacatcct ggcccacggc tttgctaaaa cagccctctt tttagggagc
     9601 ggttactgga ttaaaaagat caaaagccgg actatttatg atttcaccaa tgcctggcag
     9661 aaagataaaa tcaattcggt gttaacgatc ttcggtttgg cagtcctggc gggagtgcct
     9721 ttactggccg gctataatag taagacatta ataaagtatg ctgctggcaa aagcatattt
     9781 cttaaatatt tattccattt attaagtatt atcacctata tctatgtatt tagattttta
     9841 agatggagta tcttcaattt tgataaaaag gaagaaatta ataaggacaa tatatataac
     9901 gaaggcaaga ctattttttc aataacatta ccgattgtat taatattagt gattggattt
     9961 ttaagtaatt caattattga gattgattat acggattatc atttgtttga tggattgatt
    10021 aaaaacatag tctatatatt aatttcatta ggctttttaa aaatgacaaa ttgggtatat
    10081 gtaaaggaaa aaccagttcc ttcgatggat ctgctcttaa taaggttaaa aaattggtac
    10141 cacagtttta tggggcaaga aaaagtagaa actttagaag tggacatgga aaatcaaatt
    10201 tataatactt tgattcatat ttctgaattt gtcgataaaa ttttaccaaa aaaaatacaa
    10261 agtcaattac tattaatccc gattgtagta ttacttttat tacttttcgt ttaaaagata
    10321 aataatggat aatcaatcat cttgacatta aataacaatt aacttataat aattaataat
    10381 tgaaaatacc tctattaact gtaacaagag gtattaataa tgaaatcttt aacagaagga
    10441 gtattttctt atggctgaag aagtattatt aacagaaaaa ggttatcaaa aattagaaca
    10501 agaactagaa agattaacca cagaaaaaag aagagaagtt gcaaaaagaa tcaaagttgc
    10561 ccgtgaattc ggagatatca gtgaaaactc cgaatatgat gatgccaaga atgaacaggc
    10621 atttgtagag ggcagaatta aagaaataaa acaattaatt gataatgcca aggtaatcaa
    10681 agaagatgaa gtaagtgatg ataaggttaa cttgggtacc acagttagac ttcacaattt
    10741 agatgaagat agttatgtaa cctataaatt ggtaggaact gcagaagcag atccactcga
    10801 tcacaaaatt tcaaatgaat ctcctattgg caaagccata ttaggtaaaa ctgtcggttc
    10861 aaaattaaaa gtcaaggtac ctaagggtac aattagttat gaaataatat ctattgaaaa
    10921 atccagtgaa taaggaagag ggatagaatg tctgaagaat tcgaaaaaga tttgaataag
    10981 cttatgcgtc aaaaaattga aaaaatggaa gattttagag agcgcggtat taaaaccttt
    11041 gacgaaaaat atgaagtaga tacttatgcg gaagaaatca aggaaaactt cgaagaattt
    11101 gaaggagagg ccgtagaaat ggccggccgt ttaatggcct taaggaccca tggaaaggca
    11161 agttttgctg atatccagga tatgtccggc aagatgcagc tatatgtaaa agtagaccta
    11221 gtaggtgaag acagatatga attatttacc gaattagata ttggagatat tattgggttt
    11281 accggtgaag tatttaaaac taatagaggt caaatctctg ttaaggtaaa aagttttaag
    11341 ttgttaacca aatctattag accgctacct gaaaaatttc atggtttaaa agataaagaa
    11401 atgagatacc gccagagata tttagatcta attatgaatc cagaagttaa agatactttt
    11461 gagattagaa gcaagataat tagagctttt agagatatat tagaagaaaa agacttttta
    11521 gaagtagaga ctcctatgat gcatcccatt ccagggggag caaatgcaaa accctttgta
    11581 acccatcata atgcattaga tatggaatta tatttaagaa tagctcccga actttattta
    11641 aagagactaa tagtgggcgg ttttgataga gtttatgaaa ttaataggaa cttcagaaat
    11701 gaaggtatgt cctataaaca taatcctgag tttacaactt tagaactata tcaagctcag
    11761 gcagactacc ataatatgat ggatattacc gaagaactag tagtcgagtc tgctaagcaa
    11821 actcttgaaa caactgaaat tacttatcag gaacaggaca ttgatttaag tgttccctgg
    11881 cagagagtta caatgaaaga actggttaag aaaagaaccg gttttgatta tgatgagttg
    11941 gccgatgaag aagcagctta caaaaaagca gaagagctcg gtgtaaagat agaggaaaag
    12001 ggtctatcaa aaggggaagt attatatgaa gtatttgaaa cccgggttga ggaagattta
    12061 atccaaccaa cctttgttat ggattacccg attgaggttt cacctttagc caaaaagaaa
    12121 gaagataatc ctaatttcac ttatagattt gaagcattta tcaacggctg ggaaattgct
    12181 aatgccttta gtgagttaaa caatcccttt gaacaggaaa aaagatttga acatcagatg
    12241 gaacagagag aagccggtga tgaagaagct cagatgatgg atagagactt tattagagct
    12301 ttagaatacg gcatgccgcc aactggcggt ctggggattg gcttagatag attaattatg
    12361 ctgatgaccg attcaaaatc aataagagac gtaatattat tcccaaccat gagacccgaa
    12421 caacagtaaa taatatataa atataaaagc cagagttgtt ttcaactctg gcttatttaa
    12481 tacctgctta ttcaatatat aatattttct gtttcagaac agcgattaca acataacaga
    12541 ctatgagaga aggtataagg taacttccat tataaattat ggaatagatc cagacatttg
    12601 ccccttctgg tgcatactga gaaaagaaga ttactcccga aagtacgtgg gttacaaacc
    12661 ttaaaaatcc acctaataat agagccaata taatataaat attaattttc ttatctttaa
    12721 attttttaac ctgttcttta aagatgccag cagcacctaa catcatataa ggtaaaggat
    12781 aatctaaaat taactgggcg ggatggataa tataaggatt aaaggcaagc tgtagtaatc
    12841 cgtaaacaaa acctgctaat aagccttctt taaaacccca tcttaatgct ataattaaga
    12901 taggtaacat ttctagacta actgaaccac cctgtggcat cttccacaaa ggaagaaagt
    12961 taaaaataac ggcgagtgcg acagcaacag cgatttctgt aattgctctt agacttattt
    13021 tcataaaaaa accctcctaa ttttaattgg aggaacaata ctgagtaaat agtcccttcg
    13081 cctgcattac caggatcagg tattgtgggt cgatgtaatt tacatcctct cagcgaattg
    13141 ctccccagtt tacactatta acacttctat atattagtga taataccttc taagtgaaga
    13201 aaataattaa taacttatta tcaacaatac ataaaatgag gtgaattcaa tgaaaatttt
    13261 ggtagctccc gattctttca agggctccct aaccgcgtta gaagtagctg aatcgatagg
    13321 aaaaggtatt aaaaattatg aaagcaatat caaagtggat ttacttccct tagctgatgg
    13381 gggggaaggt acagttcaat ccctagttga tgcaacccag ggagagatta ttaaagaaaa
    13441 ggtgactggt cctctggggg aagaaataaa aactaattac ggtattttag gtgatggtaa
    13501 aacagctgtt attgaaatgg cagccgcctc aggcttacct ctggtaccgg aagaaaaaag
    13561 agatcccact aaaaccacta cctatgggac tggcgagtta atcaaatctg ccttagaaca
    13621 tggggttgaa gagatcatca tcggacttgg gggtagtgcg accactgatg ccggagtagg
    13681 tatggcccag gcgttaggag taagtttttt agatgaaaaa ggtgaagaag taggctttgg
    13741 tggtaggtat ttagataaaa taaatgagat aaggatggat aatatagata atagagttaa
    13801 agatactaag ataagagtag cctgtgatgt agataatccc ctttttggta aagatggggc
    13861 tgcctatgta tattcacctc aaaaaggtgc tgatcaagag atggtaaaag tattagataa
    13921 aaagttaaga aagtttaatg aaaaggttaa agaacgtcta aataaagatg tcaataaaat
    13981 tgcaggggcc ggggctgccg gtggtttagg agcaggcttg gtagcttttc taaatgctga
    14041 attagaaacc gggatagata ttatattaga tactgttgaa tttgaaaaaa gattaaaaaa
    14101 tgtagattta gtgattacgg gagaaggaag gttggatgga cagtcaattt atggtaagac
    14161 tcccatcggg gtagccaaaa gagctaaaaa tcataatatt ccagtcatcg caatagcagg
    14221 ttctttagga ccgggggcaa aaaaggtttt agataaaggc attgatgcat attttagtat
    14281 aatagataag cctgataatt taaaagcagt cataaaaaga agcaatgagc ttttagaaag
    14341 tactgccgaa caaattataa gaatattttt aataaattat taaaaagaca tattaatcta
    14401 cttgcattta ggccttttta atatttataa taattataga gatcaaaaaa caaggaggtt
    14461 aaaaacatgt ttaaaagatt agaagtgaaa agttcaagaa ggaatgatat ggttgatatt
    14521 actaatcagg ttttgaattt tattaaagaa tcaaatgtac aggacggaat cttgattatt
    14581 aatgtcctac atacaaccgc agccgttact attaatgaga atgcagatcc tgatgttaaa
    14641 aaagatataa taaatgaatt tgcaaagttt gttcccaaac atgataatta caaacatata
    14701 gaaggtaatg ctgatgccca tattaaaaca agtatgatgg gaaataacga aactattatc
    14761 ataaaaaatg gccgcttaaa gctgggtagt tggcagggaa tattcttatg tgacttcgat
    14821 ggtccgagaa caagaacagt aattttaaaa ataatggaag attaattgaa tttccccttg
    14881 acagagcggg ggttagttga taagatataa aatgtcgctg agggaaaaag gcgttaaaaa
    14941 aaagaggcca aaaacccctt gacagcggcc gcgaaaagtg ttaatattat ttcgcggtag
    15001 agaaatagaa ccttgaaaag tgaacagcag accctgacag ttcttaataa gtttcaagta
    15061 agaactttca atagcg
//
LOCUS       DATGCJ010000049         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_452859, whole genome shotgun sequence.
ACCESSION   DATGCJ010000049 DATGCJ010000000
VERSION     DATGCJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452859"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VKN64_02315"
     CDS             <1..1021
                     /locus_tag="VKN64_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554130.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HMA59098.1"
                     /translation="GGIKKFQWVTIPLALRGMVVKEDGTKIEISIGEKEDDPVFYISD
                     ILPHLGKKQMEKKMKEAINAQHLNLVVGSIPLRDEEEENENGNDKIKAAILEHLEDEY
                     DVTGEDLVSADLQIVPADGVKDIGFDRALLGGYGHDDRVCSYTGLRALLDINVPEYTA
                     VTLLVDKEEIGSMGATGMQSRFFENFVAEMSSRYYNSYSELDLREILENSSSLSGDVN
                     AAFDSNFADVFDKRNAAFLNKGIVLTKYTGARGKAGSSEATAEFMAKVRGLLNEAGVI
                     WQVAELGKVDEGGGGTIAQFLANYNMDVLDAGPAVLSMHSPYEVVAKIDVYNTYLAYL
                     TFLEK"
     gene            1074..2837
                     /locus_tag="VKN64_02320"
     CDS             1074..2837
                     /locus_tag="VKN64_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMA59099.1"
                     /translation="MTKYSFNTKKILVYTLIIIALLAGYYYNNYSMIDNFIYDNYQKL
                     AYIFSSQNKESQVTILSINNITYNKLGGPPLDRNYFVDTIAKLTEDGAKTITFDLLFA
                     QAQDAQADSLMIDRLRENKNIVMPVQIDYSPAGEGKEIDYTVNEVKAPLSSFKNLVKL
                     GHTNLISDSDGIVRNLSPLFIKNNTSYIPLARRAAEMALEEEITITEGEYLINYLGPS
                     GTIPQISLQHYLNDDYNPELIKDQIVLLGVTEGETSNQYTSIFSEDASFSEVELQAQM
                     TNNYLHQNYIEVNESGRNMLIAFVVLWLVFYLFEKMHPLRSLAVLSVISIIILTLNYF
                     LTVQYHLFTEISIYLVGIGVLYGASLVTWYGFNRKRKHEIIKKLKPYFSDFLLNKIVQ
                     NPELLKSKGDNPPVTMLLFEFSNFKWYSDMNTPQKVVEDLNHYYRNITKIIFKYDGVV
                     DKYLGDGILAYWSKGFEQDNHRNRAVKAAIEIMNYIEQENIELKPSIALDSGKITLGD
                     IGTKERMDFKAMGNIVHNTTELAEVTEPDEILIGENTYYGLSDIYKKLEWKYKEVEIE
                     GVERSFVVYSLKEFKSLSKGE"
     gene            2841..3458
                     /locus_tag="VKN64_02325"
     CDS             2841..3458
                     /locus_tag="VKN64_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMA59100.1"
                     /translation="MKKTLTIILISLITLTTLTAAFAQQTEEKISINMNSLKGIIYRK
                     TDQGFFEFFRVQVWTRLLDNATISQGETIKTAPDSLLTLNIGGNNKVELQEETILKIG
                     DNITPEAQKLNVKKGRVIINTNINPEQKFNIEIETPESIIKTSHAMLKVEVNDYRTLL
                     FIEEGEVTLMEKTTLQEVNLTETQIAIVENNEIKKYQSMQNQQTE"
     regulatory      3517..3591
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3723..4304
                     /locus_tag="VKN64_02330"
     CDS             3723..4304
                     /locus_tag="VKN64_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59101.1"
                     /translation="MFEKKLSVIALTLVMVLVLAPTIFAELDIDFDEYGLDISDEQEA
                     AIIEKLEELIDKNDQITEEDILAVLEKIKVEEEEETEDTPDENEEEENEIGKELSEKI
                     ETWKEEYEAEGNEEYKSKGQFLSDNIQDFLDEKLTRDGEEEEEEQNQVQDQEQEQKQE
                     EEKSQEMEEKGMPDDVKDKVEEKKSGNPGKGKK"
BASE COUNT         1637 a          647 c          870 g         1276 t
ORIGIN      
        1 cggtggaatt aagaagttcc agtgggttac catcccctta gcattaagag gtatggttgt
       61 taaagaagat ggaactaaga tagaaattag tattggggaa aaagaagatg accccgtatt
      121 ttatataagt gatattttac cccacctcgg taaaaaacag atggaaaaaa agatgaaaga
      181 agctatcaat gctcaacatc ttaacctggt tgtgggtagt attcctttac gagatgagga
      241 agaagaaaat gaaaatggta atgataagat aaaagcggct attttagaac acttagaaga
      301 tgagtatgat gtaacaggcg aagacttggt aagtgctgac ttacagattg taccggccga
      361 tggagtaaaa gatatcggtt ttgatagggc tttactcggc ggttatggac atgatgatag
      421 agtctgtagt tacaccggtc tgagagcttt acttgacatt aatgtaccgg aatatacagc
      481 tgttacactt ttagttgata aagaagagat aggtagtatg ggtgcaaccg gcatgcagtc
      541 cagatttttt gaaaactttg tagctgaaat gtctagtaga tactataata gttacagtga
      601 acttgactta agagaaattc tggaaaattc cagttcactt tccggtgatg taaatgctgc
      661 ctttgattct aacttcgctg acgtttttga caaaagaaat gctgcctttt taaataaggg
      721 tattgtactg accaagtata caggagcccg cggtaaggca ggatcctcag aagctactgc
      781 tgaatttatg gcaaaagtac gcggtctgct taatgaagca ggtgttatct ggcaggtagc
      841 cgaactcggt aaagttgatg aaggtggagg aggaactatt gctcagttcc ttgctaacta
      901 taacatggat gtacttgatg ccggtccggc tgtattatca atgcactcac cttatgaagt
      961 agtggctaaa attgatgttt ataacactta tcttgcttat ttaacattcc tggaaaaata
     1021 attaaaataa ggggaggctt ttagcctccc ttctctattc aggaggattt cagatgacta
     1081 aatatagctt caatactaag aaaatattag tttacacttt aataataata gcacttttag
     1141 cgggctacta ttacaataat tacagtatga tagataattt tatttatgac aattatcaga
     1201 agcttgccta catattttct tctcaaaaca aagagagtca ggttactata cttagcataa
     1261 ataatattac ctataataag ttagggggac caccccttga tagaaattat tttgtagaca
     1321 caatagcaaa gctgactgag gatggagcta aaactataac ttttgatctg ctttttgccc
     1381 aggcccagga tgcccaggcc gacagcttga tgattgaccg cttaagggag aacaaaaata
     1441 ttgtaatgcc agtccaaatt gattacagcc cggctgggga aggtaaagaa attgattaca
     1501 cggtcaatga agtaaaggca cccctttctt cttttaaaaa cttagttaaa ttgggtcata
     1561 caaatctaat cagtgattct gacggtatag taagaaactt atctccctta ttcattaaaa
     1621 acaatacttc atacataccc ctagccagaa gagcggctga aatggcatta gaagaagaaa
     1681 ttacgattac tgagggagaa tatctgataa attacttggg accgagcggc actatcccgc
     1741 agatttcatt acagcattat ctaaatgatg actataatcc tgaattaatt aaagatcaaa
     1801 ttgtcctctt gggtgtgacc gaaggggaaa ccagcaacca atacacttcc attttttccg
     1861 aagatgcttc ttttagcgaa gtggaattac aggcccaaat gacgaataac tacctacatc
     1921 aaaattacat agaagtaaat gaaagcggcc gtaatatgtt aatagcattt gtggttttat
     1981 ggcttgtttt ttatctattt gaaaaaatgc atcctctccg ttcactggct gtcttatctg
     2041 ttatttcaat aattattttg actcttaact actttttaac tgttcagtac catcttttta
     2101 ctgaaatttc aatatactta gttgggatag gagtccttta tggggcgagc cttgttacct
     2161 ggtatggttt taatcggaag cgcaaacatg aaatcattaa gaagctcaag ccctattttt
     2221 ctgattttct tttaaataaa atagtacaaa accctgaact tttaaagtct aaaggagata
     2281 accctccggt taccatgctg ctttttgaat ttagtaactt caaatggtac tctgatatga
     2341 atactcctca aaaagtagta gaagatttaa accactacta tcgaaatatt accaagatta
     2401 tttttaaata tgatggagtg gtagataaat atttaggaga tgggatcctt gcctattgga
     2461 gtaaggggtt tgaacaggat aaccaccgga atagagcggt taaagctgct attgagatta
     2521 tgaactatat agaacaagag aatatagaat taaaaccttc tattgccctg gattcaggta
     2581 aaataacact tggtgatatc ggaaccaaag agagaatgga ctttaaagca atgggcaata
     2641 tcgtccataa tacgactgaa ctagcagaag ttaccgaacc agatgaaatc ttaatcggtg
     2701 aaaacacata ctatggactt tcagatatat ataaaaagtt ggaatggaag tataaagaag
     2761 tggaaataga aggagtagaa agatcctttg tggtctatag tttaaaggag tttaagagtc
     2821 tgtctaaggg ggaataaatg atgaaaaaaa ctttaactat tattctaatc tccctaatca
     2881 ccttaacaac cttaacagct gcttttgcac aacaaaccga agaaaaaatt agtataaata
     2941 tgaattctct taagggtatt atttaccgta aaactgatca gggttttttt gaatttttca
     3001 gagtgcaagt ttggaccagg ctattagata atgccacaat cagtcaagga gagaccatta
     3061 aaacagcccc tgatagttta ctaactttaa atataggcgg taataataaa gtagaattac
     3121 aggaagaaac cattttaaaa atcggagaca atataacacc agaagctcaa aaactcaatg
     3181 ttaaaaaagg tagagttatt attaatacaa atattaaccc tgagcagaaa ttcaatattg
     3241 aaattgagac tccagagagt attataaaaa ctagccatgc tatgttaaaa gtagaagtaa
     3301 atgattatcg gaccctgcta tttattgaag aaggagaagt tactttaatg gaaaaaacaa
     3361 ctttacaaga agtaaactta acagaaacac aaatagcaat tgtggaaaat aatgaaatca
     3421 aaaaatatca gtcaatgcag aaccaacaaa cagaataata cttgataaat tcactaaaat
     3481 atgataattt tatatttaga taaagaaaaa aataaatatt gagaaaggca aactagttgt
     3541 gaaactagga cgcaaagcca tggatctttt taagactgcc aggttaccat atttttaagt
     3601 ctatggctta tacaagcaat aggctttttt gatttttaaa taaaaatttg ttgtaatctg
     3661 gaaaaattct aattttttct caagataaaa tttttgagtc tataattaaa agggggagta
     3721 atatgtttga gaagaaattg tcagtaattg ctttaacttt agttatggtg ctggttttag
     3781 cacctaccat ctttgcagaa cttgatattg attttgatga gtatggtcta gatatatctg
     3841 acgaacaaga agcagcaatt atagaaaaac tagaagaact catagataaa aatgatcaaa
     3901 taactgaaga agatatttta gccgtattag aaaaaataaa agtagaagag gaagaagaaa
     3961 cagaagacac tccagatgaa aatgaagagg aagaaaatga aattgggaaa gaactttcag
     4021 agaaaataga aacatggaaa gaagaatatg aagcagaagg taatgaagaa tataaaagta
     4081 aaggccaatt tttatctgat aatattcaag atttcttaga tgagaagtta accagagatg
     4141 gagaagaaga agaggaagaa caaaaccaag ttcaggacca agaacaggaa caaaagcaag
     4201 aagaagaaaa gagtcaagag atggaagaaa aaggaatgcc agatgatgta aaagataaag
     4261 ttgaagaaaa gaaaagcggt aaccccggca aaggtaaaaa ataaaaaaat aagatgtgat
     4321 tttatagata ggccacctat ttggattgtg ttccaaagaa ggtggcctat tattatctat
     4381 aaaggagttt aattatagca ataaacttgt tatatcttct gttttgattt
//
LOCUS       DATGCJ010000050        11130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_96417, whole genome shotgun sequence.
ACCESSION   DATGCJ010000050 DATGCJ010000000
VERSION     DATGCJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96417"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VKN64_02335"
     CDS             complement(<1..1542)
                     /locus_tag="VKN64_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HMA59102.1"
                     /translation="MPSASNSSTLNKIFYNVDILFAFAVVGIVIMFIIPMPTIIMDLL
                     LTANITFSLLIILVSIYINEPLQVSVFPSLLLFATLFRLGLNVSTTRLILGQGYAGKV
                     IASFGNFVVGGNYVVGFIIFLILVVIQYVVITKGAERVAEVAARFTLDAMPGKQMSID
                     ADLNAGIIDEREAQEQREKIRQEADFYGAMDGASKFVKGDAIAGIIITVINIIGGLII
                     GMAQQGLSFGEAAQTYTLLTVGDGLVSQIPALLISTATGMIVTRAASERNMGEEMSDQ
                     MMSQPKALFIASIVLFILGFMPGLPTVPFFVMALIMGGLGFVLRSTIKEETEEIEEEE
                     EPSPQEMQQARENEIKNIIKVDQLEVEVGYNLISFVLPDQGGDFLDRVSMIRKQMAME
                     LGIIIPPVRILDNLQLEPNIYRVKLKGVEISRFEILPDYYLAMDSGMTTADIKGIETK
                     EPAFDTPAIWIDESQKEEAEMANYTIVDPPSVMATHLTELIKEHAHELIGRQEVKDLV
                     DNVEEN"
     gene            complement(1575..2639)
                     /gene="flhB"
                     /locus_tag="VKN64_02340"
     CDS             complement(1575..2639)
                     /gene="flhB"
                     /locus_tag="VKN64_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HMA59103.1"
                     /translation="MAENSTGQKTEEPTPKRKREAREKGQVAKSQELNQAFTLLASFS
                     VLYILFSGMMDSLTMEVADHLSFQTVTTLDIATAYNILMETIFYVVSLVAPVMIASAV
                     MGIIINFTQVGPLFSGESLKPKLDSLNIIKGFGKLFSLKSLVEMVKSVLKIIIIAVIA
                     YFYLRNSILDLITMTEQGLQPALMMIGTIIYKAAVAITIFLVGVGVLDFMYQKWEHKK
                     NLKMTKYEVKQERKEMEGDPQLKQKRKEKQRQMSVNRMMSEVENADVVIANPTHIAVA
                     LKFDIKEMEAPQVLAKGEGYVALKIKEKAKELDIEVVENKPLARSLNKMCEIGDFIPE
                     ELFEAVAEILAYVYKKENRY"
     gene            complement(2657..3445)
                     /gene="fliR"
                     /locus_tag="VKN64_02345"
     CDS             complement(2657..3445)
                     /gene="fliR"
                     /locus_tag="VKN64_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HMA59104.1"
                     /translation="MAANLLAPNLIYLFFLIMARYFGIFLITPIFSSVAILRQVKVVL
                     ALLMAVITFLLTYQAANIAAPAHDLEVILYLIKELSIGIFMGFIIYIFFAAIQLAGQF
                     IDLRMGFRIANVVDPMTGASSPIIGQFKNIFTILVFLSINGHLMIIKSLNKSFDIIPL
                     GQVSYDANLWRFLFRRSADLFIIGFKIALPIMGTLFIVDIMLGFLARAVPQMNIFIIG
                     LPLKILIGFILLILSFEILVYYFSDILNNGLDQIIQLINILAPG"
     gene            complement(3483..3749)
                     /gene="fliQ"
                     /locus_tag="VKN64_02350"
     CDS             complement(3483..3749)
                     /gene="fliQ"
                     /locus_tag="VKN64_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HMA59105.1"
                     /translation="MDQSAVIDMGRQSLYIVLKVIAPVLGAGLLTGLIVAILQATTQI
                     QEQTLAFVPKIFAVLAAIAFLGPWILTTVVDFVTELFQSIPLYM"
     gene            complement(3763..4536)
                     /gene="fliP"
                     /locus_tag="VKN64_02355"
     CDS             complement(3763..4536)
                     /gene="fliP"
                     /locus_tag="VKN64_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552747.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HMA59106.1"
                     /translation="MIQNNKQYLVLILIIFIMFSLNIDTYAQEGFNIPDVSLDISSPN
                     QSGEGNDLVLSLRILGLLTVLSLAPAIVIMFTSFTRVIIVFSIIKRALSIRNMPPNQV
                     LIGLAIFLTIFIMAPVWQTVNTEALQPYLNEEISIDQAYNRTEEPLRDFMFTQTSEDS
                     IALFVEMSEMERPENQADIPLYVLIPAFLTNEIKIAFQIGFMIYIPFLMIDMIVASIL
                     MSMGMMMLPPVIISLPFKILLFVLVDGWHLIINSLVQTF"
     gene            complement(4520..4882)
                     /locus_tag="VKN64_02360"
     CDS             complement(4520..4882)
                     /locus_tag="VKN64_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HMA59107.1"
                     /translation="MNYTFEILKIGFYLLVIVGLIYFLSTFFKKNVLNVQSGKHMKLI
                     DKLYMGSKTGLFLVEVNDEIILISQTENRIEKLDKWNTVDFDYDVEELKEGNTNTQAQ
                     FKEFFRKYLGRNDSDSEQ"
     gene            complement(4894..6042)
                     /gene="fliY"
                     /locus_tag="VKN64_02365"
     CDS             complement(4894..6042)
                     /gene="fliY"
                     /locus_tag="VKN64_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HMA59108.1"
                     /translation="MSNGNGQLSQEEIDSLINAGDDEVGEGNQLTEDEADIIGEIGNI
                     AMGSAATALFTLLNKKVDITAPKVDISTFKEVVAEYSEPFVLVKVEYIEGLEGLNLLI
                     LDSKDAAIIADLMMGGSGENVDENTELDEIHLSAVGEAMNQMMGSSSTSISNIIDKVV
                     NISPPEPIFDVDADEFENDYFDKDDSLVVTSFDLKIGNLIDSTFKQLAPLKFTKKLIK
                     NLKSNNEGLTAKSDNSMQSNISEVAAENTQKQKEEKKQPAKAAANAGSHSSSQQLKSS
                     EKVDVQEAQFPQFGEKEGVSLPQNMELLKDVPLTVTVRLGKTRMTISEVLDLGEGSII
                     ELDKLAGELVDLYVNGKLIAKGEVVVIDESFGFRVKDIISPAERISDL"
     gene            complement(6035..7045)
                     /gene="fliM"
                     /locus_tag="VKN64_02370"
     CDS             complement(6035..7045)
                     /gene="fliM"
                     /locus_tag="VKN64_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HMA59109.1"
                     /translation="MADSEVLNQNEIDSLLSALDSGNVDVEEMKESSESDNVKKYDFR
                     RPNKLSKEQMRTLQMIHENFARLLTTTLSTRLRSMVNFEVASIEQLSYREFIRSLPEP
                     TIIGISELNPLDGQFIFEINPEVGFTIIDRLFGGLGKPIEEIRSFTDIEQVVLKKSIN
                     WVMESFKEAWENLLDINPKLRELESNPQFTQIVPDNDMTIIITLSATVADSEGLVNIC
                     IPYIMLEPIVSQLNAQHWFSSTRKEKTSEHIEKLRERVKKAKVDMYAELGATDLTVED
                     ILYLKKGDIVKLNKKVKEPIDVRVGKIKKFKAIPGNFNKHHALKIVRVETEVKEEGDL
                     DE"
     gene            complement(7058..7531)
                     /locus_tag="VKN64_02375"
     CDS             complement(7058..7531)
                     /locus_tag="VKN64_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HMA59110.1"
                     /translation="MAEEEGNNGLMRLIIIAVSIVLVLSIIGFVMVKFVNPEQVSLGS
                     VTGKESEQKEEKDEIGPTYQLGDFTVNFAENQNYQFLKASIVFEVSHAKLNEELNKRN
                     PQIRDLVISILRTQTPDDIKAPTTDNIKSEIKTKVNDKLNTGEIKNVWFTQLVIQ"
     gene            complement(7534..8274)
                     /locus_tag="VKN64_02380"
     CDS             complement(7534..8274)
                     /locus_tag="VKN64_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HMA59111.1"
                     /translation="MPRRKRNSNNEENNTGSPAWMTTFGDLMTLLLVFFVLLYSFSVI
                     DLQKFQGFMSALQNQLGVMEGGQTISESDVLDEGMQGEYFNPSQTNLGKVYKNMQGFI
                     AENELQDKVSVEMTKKGLVIRMTGEILYDIGEAIIKPTGKKVLDEIGRNIEGINNNVK
                     VDGHTDNLPISNSQYPSNWELSTARAVKVIKYFIENGDINPARLSAAGYSKYRPLKKN
                     TNPSNRAVNRRVEVVIMNTTINQEVGSE"
     gene            complement(8267..9073)
                     /locus_tag="VKN64_02385"
     CDS             complement(8267..9073)
                     /locus_tag="VKN64_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMA59112.1"
                     /translation="MNLSTVIGIALGIILVVGAIFLGGNAIIFLSVQSIMIVLGGTLA
                     GTFVSFSLSDLKKIPSLLKIAFTDNDIDSDSIIEILVNLAKKARREGLLTLEQDIQEI
                     DDPYLEKGIQLVVDGTEPELVKDIMETKLAYMEGRHSLGKGIFDKMGQLSPAFGMIGT
                     LIGLIQMLSELDDPSKLGSGMAVALITTLYGAFLANLFFIPLANKLKVKSQEEVLIKE
                     LMIEGILSIQAGENPRIIEEKLQAFLSKEQPGDAASEEQVDTSRVVGENA"
     gene            complement(9079..9282)
                     /locus_tag="VKN64_02390"
     CDS             complement(9079..9282)
                     /locus_tag="VKN64_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HMA59113.1"
                     /translation="MIKLVRLNGEEITVNADLIETVKSTPDTIVELTTHKRILVEDSV
                     DDVIDKVIDYKRKIFNSLMLDEE"
     gene            complement(9348..10985)
                     /locus_tag="VKN64_02395"
     CDS             complement(9348..10985)
                     /locus_tag="VKN64_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HMA59114.1"
                     /translation="MLRSMYAGVSGLKAHQQQMDVIGNNISNVNTTGFKGSRVTFKEM
                     LSQTINGASSPQGGRGGTNPKQIGLGVQLGSIDKNMGQGSLQSTGKETDVAIQGNGFF
                     IVNDGNQNLYTRAGNTSFDEEGYLVNSASGNRVQGWVANESGEIQETNMDSLEDISLD
                     ETMDASATTEASYGDNLNPTLDELNFTDGSDTFEVDDGNETDNINVSLSKGEEENVWD
                     FSLNADSEDTIFSDTSNNNYSGTLVLNADGTIKEIYDGSGTNSIAGGTISLTDVDGGT
                     TAGTDIDVASIGDHINVTNLFEGSSPDQTVNLETNNKQTVSTSIYDSQGVEHNVTFDL
                     LKNGQNQWVINESSVDVSGASATAGTGSWFGGSNHQIEFDENGKIKSGQTVNLQLTPN
                     GGAAEGQEITLDFAQLTQYEGDMTADLDQADGYSQGELKDFTINDSGTIVGSFDNGYN
                     KTLAQIGVAEFANPEGLNKSGETMFSVSNNSGYPKVGAAGSGGKGFISSSKLEMSNVD
                     LAEQFTKMITSQRGFQANSKVITTSDEMLQGLVNIKR"
BASE COUNT         3204 a         2418 c         1791 g         3717 t
ORIGIN      
        1 attttcttct acattatcta ctaaatcttt aacttcctgt cttccaatta attcatgggc
       61 atgttcttta attaactcag ttaaatgggt agccattact gagggtggat ctactatcgt
      121 gtaattggcc atctcggcct cttctttctg gctctcatcg atccagatgg cgggagtatc
      181 aaaggccggt tccttggtct caatgccttt gatatcggcc gttgtcatgc cggagtccat
      241 ggccagatag taatccggca aaatctcaaa cctacttatc tctacccctt tcaacttgac
      301 cctgtaaata ttgggctcta gctgtaaatt atcaaggatc cggaccgggg gaatgattat
      361 tcctaactcc atggccatct gctttctgat catcgaaact ctatcaagaa agtcaccgcc
      421 ctgatcgggc agaacaaatg atatcaagtt atagccgact tcaacctcaa gctggtcgac
      481 tttaatgata tttttaatct cgttttcacg ggcctgctgc atctcttggg gagaaggttc
      541 ttcctcctct tcaatttctt cggtctcctc tttaatagtt gacctgagaa caaaacctaa
      601 tcctcccatt ataagggcca tcacgaaaaa cggtactgtc ggcaggccgg gcataaaacc
      661 taaaataaat agtacaatac tagcaataaa gagcgccttc ggctgactca tcatctgatc
      721 agacatctct tctcccatgt ttctttctga agcagctctg gttacgatca taccggtcgc
      781 tgtcgagatt agaagggccg ggatctgaga aactaaacca tcacccactg tgagaagggt
      841 ataagtctgg gcagcttccc cgaagcttag cccctgttgg gccatgccga tgatcaatcc
      901 accaattatg ttaatgacag taatgataat accggcaatg gcatcacctt ttacaaactt
      961 actggcacca tccatggcac cataaaagtc ggcttcctgt ctgatctttt ctctctgttc
     1021 ctgtgcttcg cgttcatcaa taatacctgc attcagatca gcatcgatac tcatctgttt
     1081 accgggcatc gcatccaggg taaagcgagc tgctacctcg gccacccttt cggctccctt
     1141 agttattact acatactgga taacaactaa gatcaaaaag atgataaatc ctactacata
     1201 gttaccacca accacgaagt taccaaaact ggcaattacc ttaccagcat aaccttgtcc
     1261 taggataagc cgtgtggttg aaacattaag acccagtcta aatagggttg caaaaagtag
     1321 taaggatgga aatactgata cctgtaaagg ctcattaatg tagatactta ctaaaattat
     1381 taataaggaa aatgtaatat ttgctgtgag cagtagatcc attatgatag tgggcatcgg
     1441 gataatgaac atgatgacga taccgactac tgcaaaggca aacagaatat ctacattata
     1501 aaatatttta tttaatgtac tgctgttaga cgctgagggc attcaatttt acctccaaat
     1561 ttctaattaa actattaata cctgttttct ttcttgtata cgtaagccaa aatctcggct
     1621 actgcttcaa acagttcttc tggaataaag tcgccaattt cacacatctt gtttaaagaa
     1681 cgggccagtg gtttgttttc gacaacttca atatcaagtt cttttgcctt ttccttgatc
     1741 tttaaggcaa cataaccttc cccttttgcc agcacttgcg gggcctccat ctccttaata
     1801 tcaaacttga gggcaaccgc aatatgagtt gggttggcaa ttacaacatc tgcattttca
     1861 acttctgaca tcattctatt tacactcatc tgtctctgtt tttcttttct tttttgtttt
     1921 agctgggggt caccttccat ttcttttctt tcctgtttga cttcatattt ggtcatctta
     1981 agattctttt tatgttccca tttctgatac ataaaatcta agacgcccac tcccactaaa
     2041 aatatggtga ttgccacagc tgccttgtag atgatagttc caatcatcat cagggcgggc
     2101 tgtaggccct gctcggtcat ggttattaaa tccaagatcg agtttcttaa atagaagtaa
     2161 gctattacgg caattattat tatttttaaa acagatttta ccatctcaac taaactctta
     2221 agcgaaaata acttgccaaa gccctttatg atatttaaac tatctaactt cggttttaag
     2281 ctttcaccag aaaataaagg gccgacctga gtaaaattaa taataatacc cataacagcc
     2341 gatgcaatca tgaccggtgc taccagggaa actacataaa atatggtttc cattaagata
     2401 ttataggcgg tagcgatatc taaagtagtg accgtctgaa atgatagatg gtctgctacc
     2461 tccatggtca aagaatccat catgccggaa aagagaatat ataggaccga aaaacttgct
     2521 aagagtgtaa aggcctgatt tagttcctga ctcttggcaa cctggccttt ttcgcgggct
     2581 tctcgcttcc gtttcggcgt tggttcttcc gttttttgac ctgtagaatt ctctgccatc
     2641 ttctttcctt tctatattat ccaggtgcta agatgttgat taactggata atttggtcca
     2701 aaccattatt taagatatct gaaaaataat aaactaatat ttcaaaagat aagattaaca
     2761 atataaatcc gattaaaatc ttcaaaggta aacctatgat aaagatgttc atttgaggta
     2821 cggccctcgc cagaaatccc aacattatat ctacgatgaa cagggtcccc atgatcggta
     2881 aggctatttt aaaaccgatt ataaatagat cggcacttct tctaaacaga aaccgccaga
     2941 ggtttgcatc ataagaaacc tgccctaatg ggataatatc aaaagactta tttaaactct
     3001 tgattatcat caggtgtccg ttaatcgata aaaataccaa gattgtaaat atatttttga
     3061 actgaccgat aatcggggaa gaagcccctg tcatcgggtc cactacatta gcaattctaa
     3121 aacccattct gaggtcgata aactgaccgg ccagctgaat tgctgcaaaa aatatgtaga
     3181 tgataaagcc catgaaaata cctatcgaaa gttcttttat caaatacaag atcacttcta
     3241 aatcatgagc gggggcggca atattggctg cctggtaggt tagtaaaaaa gttattactg
     3301 ccattaataa tgctaatact acttttacct gccttaagat tgctacactg ctaaaaatag
     3361 gtgttattaa aaatattcca aagtagcggg ccatgattaa aaagaataga tatattaaat
     3421 taggtgctaa taaattggct gccataaata ttcatcctat cacatataaa gaggtatact
     3481 tttcacatat aaagaggtat actttgaaac agttcggtca caaaatcaac tacagttgtt
     3541 aaaatccagg gacctaaaaa tgcgattgcc gccagtacgg caaatatttt aggtacaaag
     3601 gctaaggtct gttcctgaat ctgggtagta gcctgtaaaa tagctacaat taaaccggtt
     3661 aagagaccgg cccctaacac aggcgcaatc acctttaata ctatatataa agactgtctt
     3721 cccatatcta taactgcact ttgatccaaa gtaaatctct ccttaaaaag tttgtaccag
     3781 tgaattaata attaaatgcc agccatctac taaaacaaac agtaatattt taaatggcag
     3841 tgagatgatt accggtggca acatcatcat tcccattgac atcaagatac tggccacaat
     3901 catatctatc attaaaaacg gaatatagat cataaaaccg atctgaaaag ctatcttgat
     3961 ttcattggtt aaaaaggccg ggattaaaac ataaagcggt atatctgcct ggttttccgg
     4021 ccgctccatt tcagacattt ctacaaataa ggcgatagaa tcttcactcg tctgggtaaa
     4081 catgaagtcc cttaagggct cctctgtcct attataggcc tgatctatac tgatctcttc
     4141 gttaagataa ggctgtaagg cttctgtatt aaccgtctgc caaaccgggg ccatgataaa
     4201 aatcgttaaa aagatagcca gtccaattaa aacctggtta ggaggcatat tccttatcga
     4261 aagggccctt ttgattatgg aaaaaactat gataaccctg gtaaaagaag taaacattat
     4321 cacgatagct ggagctaatg ataatactgt taaaagtcct aatattctca gggataaaac
     4381 taggtcatta ccctcacctg actggttagg ggatgagata tccaaactga catctggaat
     4441 attgaagccc tcttgagcat aggtatctat atttaaactg aacatgataa atattatcag
     4501 tattagaact aaatactgtt tattgttctg aatcactatc gttcctccct agatacttcc
     4561 tgaaaaattc tttaaactgg gcctgtgtat ttgtatttcc ttcttttagt tcttccacat
     4621 cgtagtcaaa atcaacagta ttccatttat ccagtttttc aatcctgttt tcggtctgac
     4681 tgattaaaat aatctcatcg tttacttcta ccaaaaaaag tcctgtttta gaacccatgt
     4741 ataatttatc tattagcttc atgtgtttac cagactgtac atttaagaca tttttcttaa
     4801 agaaagtact taaaaagtat attaatccca caattactaa cagataaaaa cctatcttta
     4861 aaatttcaaa agtataattc attttgtact cctttataga tcgctaattc tttcagccgg
     4921 acttattata tccttgactc taaatccaaa gctttcatcg ataaccacaa cttcaccttt
     4981 agcaatcaat tttccgttaa cataaaggtc aactaattca ccagctaatt tgtctaattc
     5041 aataattgaa ccttcgccta agtccagcac ctcactaatg gtcatcctcg tctttcccag
     5101 tctaaccgtt acggttaatg gcacatcctt taaaagttcc atgttctgag gtagtgatac
     5161 cccttctttt tctccaaatt gggggaactg cgcctcttgt acatctacct tttcagaact
     5221 ttttaactgc tggctggaac tatgactacc ggcatttgca gcagctttgg ccggctgttt
     5281 tttctcttct ttttgttttt gggtattttc agcagccact tctgaaatat tcgactgcat
     5341 cgaattatct gatttggctg taagcccttc attattggac ttcaagtttt ttatcaattt
     5401 tttagtaaat tttaaagggg ccagttgttt aaaagtacta tcaattagat tgcctatttt
     5461 taagtcaaat gatgttacta ctaaagaatc atctttatca aagtaatcat tttcaaattc
     5521 atctgcatct acatcaaaaa ttggttctgg gggagatata ttaactactt tatctataat
     5581 gttagaaatc gaagtagaag atgaacccat catctgattc atggcttccc caacagcact
     5641 tagatggatt tcatctaact cagtattttc atcgacattt tctccgcttc cacccatcat
     5701 caaatctgca attatggctg catctttaga atctaaaatc aataaattta gaccttctaa
     5761 accttctatg tactctacct taaccagtac aaatggttca gagtactcgg ctacaacctc
     5821 tttaaaagta cttatatcca ccttgggagc agtaatatca acttttttat taaggagggt
     5881 aaagagcgct gtggcagcag aacccattgc aatattaccg atctcaccaa ttatatccgc
     5941 ttcatcttcg gttaattgat taccttcacc aacttcatca tctcccgcat taattaaaga
     6001 atctatttct tcttgagata actgtccgtt cccattactc atctaaatca ccttcctcct
     6061 taacttcggt ctcaactctc acgatcttta aagcgtgatg tttattaaaa ttacctggaa
     6121 ttgctttaaa ctttttaatc tttcccaccc ttacatctat cggttctttg actttcttgt
     6181 taagttttac tatatctcct tttttgagat acaggatatc ttctacggtt aaatcggttg
     6241 cacccaattc tgcatacata tcaaccttgg cttttttaac ccgttccctt aatttttcta
     6301 tatgctcact ggtcttttcc tttctggtac tggaaaacca gtgctgggca tttagctgtg
     6361 aaacgatcgg ttccagcata atatatggaa tacagatatt aactaggcct tcactatcag
     6421 ctactgtagc tgataaggta ataattatcg tcatgtcatt atcaggtacg atctgggtaa
     6481 actggggatt tgattctaat tctcttaatt taggattgat atcaagtaaa ttttcccagg
     6541 cttctttaaa gctttccatc acccagttaa tcgatttttt taaaactacc tgttcgatat
     6601 ctgtgaaaga tctgatttct tctatcggct taccaagtcc gccaaataat ctgtcgataa
     6661 ttgtaaagcc cacttccggg ttaatttcga atatgaactg cccatccagc ggattaagtt
     6721 ctgaaattcc gattatcgtc ggttccggca gtgacctaat aaactctcta taggacagct
     6781 gttctatgga agccacttca aaattaacca tacttctcaa cctggtcgat aatgtggttg
     6841 tcaataatct ggcaaagttt tcatgaatca tttgaagtgt cctcatctgt tccttagaca
     6901 gcttattggg ccttctgaag tcatatttct ttacattatc tgactcactg ctttctttca
     6961 tttcttccac atctacattg ccagaatcta aagcagatag gagagaatct atttcatttt
     7021 gattgagaac ttcagaatct gccattatgg ctcctcctta ctgtattacc aattgcgtaa
     7081 accagacatt ttttatttcg cctgtgttca atttatcatt aactttcgtc ttgatttcac
     7141 ttttaatatt atctgtagtt ggtgccttta tatcatcagg tgtctgagtc cttaaaattg
     7201 aaattaccag gtccctgatc tgtggatttc gcttgtttaa ttcttcgttt aatttggcat
     7261 ggctaacttc aaatacgata cttgctttca aaaactgata attctggttt tcggcaaagt
     7321 ttaccgtgaa atctcctagt tgatatgtag gaccaatttc atctttttct tccttttgtt
     7381 cactttcttt accggttaca cttcctaaac ttacttgttc gggattaacg aacttgacca
     7441 tgacaaaacc aattatagat aaaactaaaa ctattgaaac agctattatg attaatctca
     7501 tcaagccgtt atttccttct tcttccgcca aagttattca ctccctactt cttgatttat
     7561 cgtagtgttc attattacta cttctactcg tctgttgacc gctctattgg atggatttgt
     7621 atttttcttc agcggtctat attttgaata tcctgctgca gatagtcggg caggatttat
     7681 atctccattt tcaatgaagt acttgattac ttttaccgct ctcgcagttg acagttccca
     7741 gtttgaggga tactgactgt tactgatcgg cagattatcg gtatgtccat ctaccttcac
     7801 attgttattg ataccttcaa tatttcggcc gatttcatcc agtacttttt tcccggtggg
     7861 tttaataatt gcttccccta tatcataaag tatttccccg gtcattctga tcactagacc
     7921 tttttttgtc atctccacac ttaccttatc ctgaagctca ttttctgcta taaagccctg
     7981 catattctta tacaccttgc ctaaattggt ctgggaggga ttgaaatatt cgccctgcat
     8041 cccttcgtct aaaacatcac tttcactgat cgtttgaccg ccttccatta cacccaattg
     8101 gttctgtaaa gctgacatga aaccctgaaa cttctgtaag tcaattacag aaaaggaata
     8161 aagaagtaca aaaaatacta atagcaaggt cattaaatct ccaaaggtcg tcatccaggc
     8221 cggtgaaccc gtattatttt cttcattgtt ggaattacgt tttcttctag gcattctcac
     8281 ccaccactct actggtatct acctgttctt cactggccgc atctccgggc tgttcttttg
     8341 ataagaaagc ctgcagtttt tcctcaatta ttcttggatt ctcaccagct tgaattgaca
     8401 ggataccttc aatcataagt tcctttatta aaacttcttc ctgactctta actttcaatt
     8461 tattagctag aggtatgaaa aagaggtttg ctaaaaaggc tccgtaaaga gttgttatta
     8521 aggcgacagc cataccggat cctaatttac tgggatcatc caactcactc agcatctgga
     8581 ttaatccaat taaagtacca atcataccaa atgctggcga aagctgtccc atcttatcaa
     8641 agataccttt acctaaagaa tgtcttcctt ccatataggc aagtttggtt tccatgatat
     8701 ctttaacgag ttctggttct gtgccatcca caactagctg gatgcctttt tctaaatagg
     8761 ggtcatcaat ttcttgaatg tcctgttcaa gtgttaaaag cccttctctt ctggcttttt
     8821 tagctagatt aacaagtatt tcaattatac tgtccgaatc aatatcatta tcagtaaatg
     8881 ctattttgag aagtgatggt attttcttca aatcagatag cgagaaactt acaaaggtac
     8941 cggcaagcgt accacctaaa acgatcatga tagactgaac acttaaaaat atgatggcat
     9001 taccgcctaa aaagatcgct cccactacta atattattcc taatgctata cctattactg
     9061 tacttagatt catctttttc actcctcatc caacataaga ctgttaaata ttttccgctt
     9121 gtaatcgatg actttatcga taacatcatc aacagagtct tcaactaaaa ttcttttgtg
     9181 tgttgttagt tcaactattg tatccggtgt tgatttaact gtttcaataa ggtctgcatt
     9241 aactgtaatc tcttccccgt ttaacctaac caattttatc atcaaaccac ctacccctag
     9301 gtttagattg tggggcctaa tattaggccc cacttatatt tcatctttta tctcttaata
     9361 tttaccaatc cctgcagcat ttcatcagag gttgttatga cttttgagtt agcttgaaaa
     9421 cctctctgtg aggtaatcat cttggtaaac tgttcagcta agtctacatt ggacatttca
     9481 agcttacttg atgaaatgaa gccttttcca ccactaccgg ccgctcctac tttgggataa
     9541 ccggagttgt tggaaacaga aaacatggtc tctccacttt tatttaagcc ttctgggttg
     9601 gcaaattcag ctacaccaat ttgagctaat gtcttgttat acccattgtc aaaagatccc
     9661 acaatggtac cggaatcatt aatggtgaag tcttttaatt caccctgtga atatccatca
     9721 gcttggtcta aatcagctgt catatctcct tcatattggg ttaattgagc gaaatctaag
     9781 gtgatttcct gaccctcagc agctccacca ttaggtgtaa gttgtaaatt tacagtttgt
     9841 cctgatttga ttttcccatt ttcatcaaat tctatttggt gattagaacc tccaaaccaa
     9901 cttccagtac ctgctgtggc agaggctcca gaaacatcta cactactttc attaatgacc
     9961 cattgatttt gtccattttt aaggagatca aatgtcacat tatgctctac accttgtgaa
    10021 tcgtaaatac tggtagaaac agtttgttta ttgttggttt ctaaatttac tgtttgatca
    10081 ggagaagaac cctcaaataa attggtaaca ttaatgtgat ctccaatgct cgcaacatca
    10141 atatcagttc cagcagtagt tccaccatct acatcagtga gagatatcgt gccacctgca
    10201 attgaattgg ttccactgcc atcgtatatc tcttttattg ttccatctgc atttaatact
    10261 aaggtaccac tgtaattatt gtttgaagta tcagaaaata ttgtatcctc actatcagca
    10321 ttcaagctga agtcccatac attttcctct tcacctttag ataatgaaac attaatatta
    10381 tctgtttcat taccatcatc tacttcaaaa gtatctgatc catctgtaaa atttaattca
    10441 tccagagtag gattcaagtt atctccataa cttgcttctg tagtagcaga agcatccatt
    10501 gtttcatcca atgatatgtc ttccagactg tccatattgg tctcctgaat ttcaccgctc
    10561 tcattagcca cccagccttg tactctatta ccactggctg agttcacaag ataaccttct
    10621 tcatcaaaag aagtatttcc agctcttgta tataggtttt ggtttccatc attgacaata
    10681 aagaaaccgt taccttgaat tgctacatct gtctctttgc cagtggactg taaacttccc
    10741 tggcccatgt ttttatctat actgccaagt tgtacaccta aaccgatctg tttaggattt
    10801 gtacctccgc ggcctccctg gggggaagac gctccattta tggtctgact taacatctct
    10861 ttaaaggtaa ctctacttcc tttaaatccg gtagtgttta catttgatat attgttacca
    10921 ataacatcca tctgttgttg gtgtgctttt aagccggaca cacctgcata cattgatctt
    10981 agcattttaa attatcaacc tttcctttac gctggcagaa tgcctctgtc agttagcact
    11041 ctgtggctcc cgtaaagggt cctgccatct aaccagctta atttttattt ttatttcatc
    11101 agtactgttg aatcaatatt agtaaagact
//
LOCUS       DATGCJ010000051         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_96632, whole genome shotgun sequence.
ACCESSION   DATGCJ010000051 DATGCJ010000000
VERSION     DATGCJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96632"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1684
                     /locus_tag="VKN64_02400"
     CDS             248..1684
                     /locus_tag="VKN64_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HMA59115.1"
                     /translation="MMSKNNNIKRNQIQFLAIDDLVPEDHLLRDIERAIDFDFIYDLV
                     EDKYSSNLGRPSIDPVMLIKIVLIQYLYGIRSMRQTIKEIEVNVAYRWFLGLDLTQKV
                     PHFSTFGKNYTRRFKDTDLFEQIFIKILEECFKCDYVDPTITYIDATHVKACANKNKA
                     IKKVVKKESRFYEKDLKEEIKNDRKKHGKKPLKDKKEPDDKKINVSTSDPESGLFHKG
                     EHKRVFAYTSQTACDKNGWILGYTVHAGNKHDSITFPDIYEKVKELGAQTIVMDAGYK
                     TPAIARRVIKDGITPILPYKRPMTKKGFFYKKEYVYDEYYDCYICPNNQVLKYSTTNR
                     NGYREYRSDKYVCKKCPYLDQCTKSKNHTKIVTRHIWQKYIEKCEDIRHTIGNKEIYK
                     KRKETIERIFGNAKENHGMRYTQKIGNARMKMKVGLTFACMNLKKLAKMKKKQGLLGP
                     LTPVFSQLSLLFNFKAISTKLSTLSLVA"
     gene            2182..3438
                     /locus_tag="VKN64_02405"
     CDS             2182..3438
                     /locus_tag="VKN64_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59116.1"
                     /translation="MLLNKKALVLLLTFTLVFSFSFALQAQEEVEIFSWWTGGGEEEG
                     LQALFEIFNENYPDIEIINATVAGGAGTNAKAVLKTRMVGGNPPDSFQVHGGAELIDT
                     YVKTDMMEPITPLLEEWGVKDKFNQQILDMSSYEGEIYSIPVNVHRGNIMFYNKSILE
                     EHNIEPPTNLYTFIESMKKLKEAGVTPLALGDKNKWTATHLFESIMVSTLGPFKYNGL
                     WDGTTSFDDPGIREALVAFKGIMNYVNEDHAALTWQDATRRVYDGEAAFNVMGDWAEG
                     YLKTLGWTPTEEFGWVNVPGTRGSFMVITDTFGLPKGAPHPEGAKAWLKTVASKEGQD
                     TFNPIKGSIPARIDADKSKYDPYLTDSMEDFATVTLTPSIAHGSAAPEGFVTALNDII
                     NSFITTGDVDQAQQRIKDAASEYLDI"
     gene            3497..4420
                     /locus_tag="VKN64_02410"
     CDS             3497..4420
                     /locus_tag="VKN64_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59117.1"
                     /translation="MKQKTKNLLISLGLLSPSIVLVGVFVYGFIARTVQISILDWNTF
                     AKLLRAEKDYVGLKNYLNLFMDPRFQTDLWNTLFFTGFFIIGSLTIGILLSILLNDKP
                     KGGYIFQNIYLFPMAISFVVTGTVWRWIFSPGTLPNNPYGINLLFEKLGLTSLQWSWF
                     TSTESVFFFNIALIPVIIAAIWQFSGYTMAMWLAGLRGMDEQLIEAAKIDGATPWQIF
                     RYILFPIQKPITLSAVIILAHVSLKAFALIYSMTGSGPNNVTDIPAVYMFEATFRSNR
                     YSIGSSIAIILLILVGLLIIPYLVNSFREES"
     gene            4424..>4758
                     /locus_tag="VKN64_02415"
     CDS             4424..>4758
                     /locus_tag="VKN64_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59118.1"
                     /translation="MFAKMKSKKAIIYLVLILFALFFLLPMYVLIITSFKSFAEVSVK
                     TMWDLPQQMSLNGFITALNKLAPNLLNSFYLVIPATLISAMLGSINGYVLSHWKFKGA
                     DTLFAVLLFG"
BASE COUNT         1620 a          779 c          860 g         1499 t
ORIGIN      
        1 ttgatccact atcaacagct gtattactag aaaaaaatga aagtgcaaaa gaaatgctga
       61 aatacttttt caaataagat gcaactaata ttgggctcct gaaaaggagc tccagactgt
      121 agacaaagtc acaagataag catgatatct tgtggctttt ttttgcggaa aaaggcgcta
      181 aatactatat ttatagggac tttaacccat ttttgtgata taatggatat aagaggtgag
      241 gaaaaaaatg atgagtaaaa ataataatat aaaaagaaat caaatacagt ttttagcaat
      301 agatgatcta gtccctgaag accatttact aagagatatt gaaagagcaa tcgattttga
      361 cttcatatat gatttagttg aggataaata ttcttctaat cttggacgtc ccagtataga
      421 tccagtaatg ttaataaaaa ttgtattaat ccagtactta tatggcataa ggagtatgcg
      481 acaaacaata aaagaaatcg aagttaacgt tgcttatcgt tggtttttag gattagacct
      541 cacccaaaaa gtacctcact tcagcacttt tggaaaaaac tatacccgtc gttttaaaga
      601 tacagattta tttgaacaaa tttttattaa gatattggaa gaatgtttta agtgtgatta
      661 tgtagaccca acgattacat atatagatgc aactcatgta aaagcttgtg caaacaaaaa
      721 taaagcgatt aagaaggtag taaaaaagga atcacgtttt tatgaaaaag atttaaaaga
      781 agagataaaa aatgacagaa aaaagcacgg caaaaaacca cttaaagaca aaaaagaacc
      841 tgatgataag aaaatcaatg taagtacttc tgatccagaa agcggcttat ttcataaggg
      901 agagcacaaa cgagtctttg cttataccag ccaaacagct tgtgataaaa atggctggat
      961 actaggatat acagtacatg ctggtaataa acacgatagc ataacttttc ccgatatata
     1021 tgagaaagtt aaagaattag gtgcccaaac cattgttatg gatgcagggt ataaaacccc
     1081 tgcgatagca agaagagtta ttaaagatgg aatcacaccg attttaccat ataaaagacc
     1141 aatgacaaag aagggttttt tttataagaa agaatatgtt tatgatgaat attatgattg
     1201 ttatatttgc cccaataatc aggtacttaa atactcaact acaaatcgaa atggatacag
     1261 agaatacaga agtgataaat atgtctgtaa gaagtgtccc tatttagacc aatgtacaaa
     1321 aagcaaaaat catacgaaaa tagtaactag acatatttgg caaaaatata tcgaaaaatg
     1381 tgaggatata agacatacaa taggaaacaa agaaatttac aaaaaaagaa aagaaacaat
     1441 agaaagaatc tttggaaatg ccaaagagaa tcatggtatg aggtatactc aaaaaattgg
     1501 aaatgcccgg atgaaaatga aagtcgggct tacttttgca tgcatgaatc ttaagaaact
     1561 ggctaaaatg aagaaaaaac agggattatt aggtccacta acccctgttt tttctcaact
     1621 ttctttattg tttaatttta aagctatttc tacaaaatta agcactttaa gcttggttgc
     1681 ctaatccgtt ttgtctacaa tctggggctc ctgaaaagga gctctttttt atcttggtac
     1741 ttataaatta agtactttta aaaaggtata atttgttaat tattacaaat ttaagttatt
     1801 tattataaaa tgaaggaaat caaggatttt ttaagaatta attaaattgg aaaaataaat
     1861 tataggcggg tttatctgca attgaaaaat tacgcaaaaa aagatataat tgatattaga
     1921 aagatattta atacttctca gcaatgacta ctgtttctta atattatatc caaatcattt
     1981 attgatattc ttacctttga tattatcctt tcaataaaat tttttgccaa tttatatatt
     2041 cataattttt acaatattgt aaataatgat tcccgccgca tctttctatg tattaatctt
     2101 caagagggag gtggtctaaa aaattataat taagttttaa ctagctgtac ttaattatat
     2161 aaaatttaag ggggtttatt gatgttactt aataaaaaag cgttagtatt acttcttaca
     2221 tttacactgg tcttctcttt ttcctttgcc ctgcaggctc aggaagaagt agagattttc
     2281 agttggtgga ccggtggtgg tgaagaagaa ggtctgcaag ccttatttga aattttcaat
     2341 gaaaattatc ccgatattga gattataaat gcaacagttg ctggtggtgc tggtaccaat
     2401 gcaaaagctg ttttaaaaac cagaatggtc ggtggcaacc cacccgattc cttccaggtt
     2461 cacggtggag ctgagttgat cgatacttat gtcaaaaccg acatgatgga accaatcact
     2521 cctttattag aagaatgggg agtaaaggac aaatttaacc agcagatcct tgatatgtct
     2581 agttatgaag gagaaatcta ttcaattcct gtaaatgttc acagaggtaa tataatgttc
     2641 tataacaaat ctatcttaga agaacataat attgaaccac ctaccaacct ctataccttt
     2701 attgaatcaa tgaaaaagtt aaaagaagct ggtgttacac cactcgcctt aggtgataaa
     2761 aataaatgga cagctaccca tctatttgaa agtatcatgg tttcaacctt aggtccattt
     2821 aaatataatg gtctctggga tggtacaact tcttttgatg atcctggtat aagagaagca
     2881 ttagttgcat ttaaaggaat catgaactat gtaaatgaag accatgctgc tttaacctgg
     2941 caggatgcta cccgccgagt ttatgacggc gaagcagcct ttaatgtaat gggggactgg
     3001 gctgaaggct atcttaaaac tctcggctgg acacctaccg aagaatttgg ttgggtaaat
     3061 gtacctggta cccgaggtag ttttatggtt ataactgata cctttggact accgaaaggc
     3121 gcaccccatc ctgagggagc taaagcatgg ttaaagactg tagcttctaa agaaggacag
     3181 gatacattta atcctatcaa gggatcaatt ccagcccgta tagacgccga taagagtaaa
     3241 tatgatcctt atctaaccga ttctatggaa gactttgcaa cagttacctt gactccttca
     3301 attgcacatg gttcagcagc acctgaaggc tttgtaactg ctctaaatga tatcattaac
     3361 tcctttatta caaccggtga tgttgaccaa gcccaacaaa gaattaaaga tgctgccagt
     3421 gaatatctag atatttaagt acttaaaaag ccccctgtaa gtggggggct ttcttatttc
     3481 atggaggtga ttttaaatga agcagaaaac aaagaactta ctcatttctc tgggactcct
     3541 ttccccctca atcgtcctgg tgggtgtttt tgtttacggc tttatagcgc ggaccgttca
     3601 gatatctatt ttagattgga atacttttgc taaattatta cgggctgaaa aggactatgt
     3661 gggattgaaa aattacttaa atctttttat ggatcctaga tttcagaccg atctctggaa
     3721 taccctcttt tttactggtt tctttatcat tggttcccta actatcggta ttcttttatc
     3781 aatattatta aatgacaaac caaaaggggg atatattttt caaaatattt acctgttccc
     3841 catggctatc tcttttgtag ttaccggtac agtttggcgc tggatatttt ctccgggtac
     3901 tttacccaat aatccttatg gtataaactt actatttgaa aaactcggcc ttacttcact
     3961 gcagtggagc tggtttacaa gtacagaaag tgttttcttt tttaatatag cccttatccc
     4021 cgtgataatt gctgccatct ggcagttttc cggttataca atggctatgt ggttggcggg
     4081 attgagaggc atggatgaac agttgatcga agctgctaaa attgatggag ctacaccctg
     4141 gcagatattt agatacatcc tcttccctat ccaaaaacca attacccttt cagccgtgat
     4201 tatcttagct cacgtctcat taaaagcatt tgccttaatc tattccatga caggcagtgg
     4261 ccccaataat gttaccgata ttccggctgt atatatgttt gaggctacct ttagatcaaa
     4321 taggtattca atcggttcaa gtatagctat tatattatta atcttagttg gattattgat
     4381 tataccttac ctggtcaata gttttagaga ggagtcctaa aatatgtttg ctaaaatgaa
     4441 atcgaaaaaa gccataatat atcttgtctt aatattattt gctctcttct tcctgctgcc
     4501 gatgtacgta ttgataatta caagctttaa aagctttgct gaagtttctg tcaaaaccat
     4561 gtgggactta cctcagcaga tgtccttaaa tgggtttatt acggccttaa ataagttagc
     4621 acctaactta ttaaacagtt tttacttagt tatacctgct accctaatct cagccatgtt
     4681 aggttcaata aatggttatg tcctctccca ctggaagttt aaaggggcag atacactttt
     4741 cgctgtctta ttatttgg
//
LOCUS       DATGCJ010000052         9139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_274533, whole genome shotgun sequence.
ACCESSION   DATGCJ010000052 DATGCJ010000000
VERSION     DATGCJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274533"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VKN64_02420"
     CDS             complement(<1..210)
                     /locus_tag="VKN64_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HMA59119.1"
                     /translation="MADFEKMADAVINGKQDLVDELTQKAVDEGTEPNEIINKGLING
                     MNIVGKRFKAGDMFVPEVLMSAKAMK"
     gene            complement(240..1730)
                     /locus_tag="VKN64_02425"
     CDS             complement(240..1730)
                     /locus_tag="VKN64_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HMA59120.1"
                     /translation="MSRYVRSRYNVNKTVNFQSLTEQEREEIFYAALEVLERTGVKIF
                     DDESREYLEEAGCWVEDKVVKIPISLSEWAVDSAPSKILLYDRDGNRKLNIHDNKTYY
                     GPGPTNTYHKDPDTGERRRPVLEDTVRVGKVVDALPNIDFAMDLGTPTGVTDNIADVY
                     AFKALVENTTKPIVHWGFDIDQYQDIIDIASAVRGGLEELQKKPFMFLYSEPTSPLLH
                     SREAIAKVVFAAKNKIPIVYTPCVMSGATTPATMAGTIVQGISESIAGIVVSQLVRKG
                     TPIIMGGVYGIMDMKTTIYSYGSPEFHLLQAGIAEVAHYMGIPIFGTGGCTDSHTLDG
                     QAAAESAMSILMAAQSGANMVHDVGYTASGQVGSLDQLVMGDEIIGMVKRIMRGIQVD
                     DEHLAVDVVDEVGPGGHFLGEDHTVKHFRDETWFPTLINRMRYDGWKSQANSSTMGER
                     IKEKVHDIVENHEVKPLSDEVKAKIDEIIEKAEEREANRDKEKKNK"
     gene            complement(1749..2387)
                     /locus_tag="VKN64_02430"
     CDS             complement(1749..2387)
                     /locus_tag="VKN64_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HMA59121.1"
                     /translation="MADYEKMADAVISGNQDTVVELTEKAVDEGIEPNEIIEKGLING
                     MNVVGKRFKAGDMFVPEVLMSAKAMKAGMNIVKPLLLEGEMPSEGKVLLGTVSGDLHD
                     IGKNLVGMMMESGGLEIVNLGTDIDPDEFAEKVREHKPDVLGMSALLTTTMLAMRDTI
                     EVLEEEGLRDSVKIMVGGAPVTKEFADEIGADGWAPDAASAKDLAFELINEK"
     gene            complement(2538..4103)
                     /locus_tag="VKN64_02435"
     CDS             complement(2538..4103)
                     /locus_tag="VKN64_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HMA59122.1"
                     /translation="MRKSWSENKTFWIAFILTILFVLWGVFGIDSLRVAADVSFNFVT
                     QQLGWSFMLGGTLFVLVAIYIMISKIGEIKLGDPDEEPKYTRFEWFSMLFSAGMGIGL
                     VFWSVSEPIWHYIWPATGAAANATAETQQAAINAMRLSFFHWGIHPWSIYIIVGGSLA
                     YFSYHKGLPMLLSSTLEPIIGKENLDTGWGRLINIIGVYATLFGLATSLGLGAMSIVA
                     GLAQLNWIASASTGAQIVVVIVVTAAAVISTITGIDRGIRYLSKVNVWIMSILMIFVV
                     LIGPTLFILRLFTNSLGQYLQHIVEMSFMSDAIMDNAWLPAWTIFYWAWWIAWAPFVG
                     TFIARVSRGRTIREFVIGSLFVPVLVSLIWFAVFGGTGLHVALGEGGQGLIEAVEQSE
                     AAGFYAALATLPLAKFLIFLGTLSVTLFFITSSDSATYVNGMLTSGGNPNPPRPLRVF
                     WGVSEGAIAAILIFTGGLSALQTASVVSGFPFMIIMFFMIYCLLKDLHLERKKKAKGV
                     GKYEDIEEEPELA"
     gene            complement(4339..5010)
                     /locus_tag="VKN64_02440"
     CDS             complement(4339..5010)
                     /locus_tag="VKN64_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59123.1"
                     /translation="MIQFSQDDIKKKRILRAFINSVRKVINEEGIENVTIRKIAKVAG
                     YNSASIYNYFDNLNQLIFFGSISFLDDYVKAIPFYLNKSEDELDKYLLIWECFSKNSF
                     ENPNIFYAIFTENIGDSSENLFKKYYQLFPEELDNIPKRYIPMLLESELSKRCSIHIK
                     PCIEAGYFTTEVAEEVDERIRLIYHGMLSLMVNNRLEYDPKQATATFMKYISSIIDNK
                     ALLKS"
     gene            5323..6021
                     /locus_tag="VKN64_02445"
     CDS             5323..6021
                     /locus_tag="VKN64_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59124.1"
                     /translation="MIDFDKDDVKVKRIIKLFVSSTIDIIEESGLEQVTVREVAKISG
                     YNPATIYNYFDNSRQLIFFASLNFMKDYFKGIEKIIEQDQDPLDNYLNLWREFCRYSY
                     QNPKIYYSIFGENIDESPKVLIEKYFKLYPDDFKYSPADYLPVLTDSFAKDNVTKPLR
                     ICVNKGCFTLKQAKKIDEVNMFFYQGMLSLLVNQRVSYSVEKATKKTVDHIKQVINNV
                     VLENSEKRLEEIDF"
     gene            6056..7201
                     /locus_tag="VKN64_02450"
     CDS             6056..7201
                     /locus_tag="VKN64_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMA59125.1"
                     /translation="MREIYLDNAATTRHKPQTVINAVQEFMTELNCSPGRGGYECAIQ
                     AGRQIMDTRFLISDFFNAGEVESILFTKNVTESLNILIKGLLKDGDHVITTSLEHNAV
                     LRPLNQLEEKDMIEVSYVEADQEGILDPSLIEDAVKDNTKLIILNHASNVTGTILPAA
                     EVGEIAKKHDIFYALDTAQTAGVLDIDFEKLNLDFLGFTGHKSLLGPQGTGGMAISER
                     LADEMETLIAGGTGSLSETVKQPDFLPDKFESGTLNTPGIVGLKAGIEYINEVGIKSI
                     HNHEIRLLERFLEGLKNLDKVTVYGSTDLDKKVPTVPLRVKGYDLGDLGFKLAEKFGI
                     MVRSGLHCAPLAHKTIGTFPDGALRFSIGWHTTEEDIDYALETLAKL"
     gene            7246..8076
                     /locus_tag="VKN64_02455"
     CDS             7246..8076
                     /locus_tag="VKN64_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMA59126.1"
                     /translation="MENKLNLKEKGTIVFKRLVKRYINHDISAYAALMAFFLTLSLFP
                     FLIILFAVLGQLSLDTNWIISALELFFPSEVHGLVMEFIRQNIILKDFSVLSLSVVGA
                     LWASSRGIRALMLSLNMAYEVKETRNYFVVKLIDILYTLLIIIGIVFLLTLPNIGREF
                     LRYIDTYITIDSRLIELIGIFKLTALPVTIVLIISTLYMLLPNKKQPFKKVVPGTIFT
                     VIGWTILSYLFSLFVNYIANYSLIYGSLSTIIILMLWLYFSSIILILGGELNSVVKEI
                     "
     gene            8114..8689
                     /locus_tag="VKN64_02460"
     CDS             8114..8689
                     /locus_tag="VKN64_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59127.1"
                     /translation="MSDMVKHVIVGDMLGTLSAYFNKPIINNSVFAFGLGFISHAVLD
                     YTENDYVVNWFNKSEFTAALPFLIFQITVTAILLWVFIYNNKKYSSEYTRLRIIAVIG
                     AISPDLIDALYSTINPAAWYNGQLIFPWHSLQAGSDKFIMSMYTTMAITVVFIITRYL
                     FYKYLFTKELIYPEAKEHHLQETPVEDSYPN"
     gene            8910..>9139
                     /locus_tag="VKN64_02465"
     CDS             8910..>9139
                     /locus_tag="VKN64_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59128.1"
                     /translation="MKILNYGIDIFLIGYMIYFLLFVFLGLRTKKNNDLKEPANRFAV
                     VIPAHNEEKVIDKLIENLRNLNYPKDLYDIYVV"
BASE COUNT         2793 a         1775 c         1511 g         3060 t
ORIGIN      
        1 tttcatcgct ttcgcagaca tcagaacttc tggtacaaac atatcgcctg ctttaaatct
       61 tttacctaca atattcattc catttattaa gcccttatta attatttcgt tgggctctgt
      121 accttcatca actgcttttt gagttaattc atcaaccaaa tcctgtttac catttataac
      181 ggcatcagcc atcttttcaa aatcagccac taatctatac ctccttaagg tttttatccc
      241 tatttatttt tcttttcttt gtctctgttt gcttctcttt cttctgcttt ttcaataatt
      301 tcatctattt tagcctttac ttcatcagac agaggtttaa cttcatggtt ttctacaata
      361 tcatgtactt tttctttaat tctttcgccc atagttgaac tattggcctg ggacttccag
      421 ccgtcatatc tcattctatt gatcagagtt gggaaccagg tttcatctct aaagtgttta
      481 acggtgtggt cttctcctaa gaagtgtcca cccggaccaa cctcatctac aacatcaact
      541 gcgaggtgtt catcatcaac ttgaatacct ctcatgattc tttttaccat accgattatt
      601 tcatcaccca tgaccagctg atcaagtgat ccaacctgtc cagaagctgt ataaccaaca
      661 tcatggacca tgttagctcc ggactgagct gccatcagaa tactcatcgc tgattcagct
      721 gcagcctgtc cgtcaagagt atgggaatct gtacagccac cggttccgaa gatcgggata
      781 cccatataat gggcaacttc ggcaataccg gcctgaagta gatggaactc gggtgaacca
      841 taactataga tcgtggtctt catatccatg attccataaa ctccacccat aatgataggt
      901 gtacctttac ggaccagctg ggaaactact attccggcaa tactttctga aataccttgt
      961 actatagtac ctgccatagt ggcgggtgta gtcgccccag acattacaca gggtgtatag
     1021 actatcggta tcttgttttt agctgcaaat actactttcg caattgcttc gcgtgagtgt
     1081 aataaaggag aagttggttc agaatagagg aacataaatg gttttttctg aagttcttct
     1141 aatcctcccc ttacagcaga agcaatatcg attatatcct ggtactgatc gatatcaaaa
     1201 ccccagtgaa ctatcggttt tgttgtattt tccactaatg ctttaaaagc ataaacatca
     1261 gcaatattat ctgtaactcc ggtgggagta cctaaatcca tcgcaaagtc tatgtttggt
     1321 agggcatcta caaccttacc tacccgaact gtgtcttcca ggactggtct ccttctttca
     1381 cctgtatctg gatctttgtg ataggtattg gtcggaccag gtccataata tgttttatta
     1441 tcatggatat ttaacttgcg atttccatct ctatcataaa gtaggatctt ggaaggggca
     1501 ctatcaacgg cccattcact taaactaatc ggtattttaa ctactttatc ttctacccag
     1561 cagcctgctt cttccaaata ttcacgagat tcatcatcaa agatctttac cccggttctt
     1621 tccagtacct cgagggctgc ataaaatatt tcttctcgtt cctgctcagt taaagactga
     1681 aaatttactg tcttatttac attatatctt gagcgtacgt atctactcat cttttcaccg
     1741 tcctttcatt acttttcatt aatcagttca aaagctaaat ccttggctga agctgcatca
     1801 ggtgcccagc cgtctgcgcc aatttcatct gcgaattctt tcgtgactgg tgctccacca
     1861 accattatct tgactgagtc tcttaagcct tcttcttcta agacttcaat tgtatcacgc
     1921 attgctaaca ttgtggtagt taagagtgca ctcatcccta atacatctgg cttatgttct
     1981 ctcacttttt cagcgaactc atccggatca atatcggtgc cgaggttaac aatttctaat
     2041 ccaccacttt ccatcatcat acctaccaag tttttaccaa tatcatgtag gtcacctgat
     2101 acggttccta aaagaacctt gccttccgaa ggcatttcac cctctagtag taatggcttg
     2161 acgatattca tgccggcctt catcgcttta gctgacatta gtacttccgg tacaaacatg
     2221 tcccccgctt taaatctctt accaacaacg ttcatgccgt tgatcaaacc cttttcaata
     2281 atctcattag gttcaattcc ttcatcaaca gccttttcag ttagttctac tactgtgtcc
     2341 tgattaccgc taatcacggc atcagccatt ttttcatagt ctgccaatat tattccctcc
     2401 tcttaattaa tagttttttt gtttaaagat aacctagatt tgaataacta atcatgatta
     2461 gatgaatctt aaaaaaatat ggggaagctg gaatgttagc catattccag cttttcccct
     2521 ttatttactt ttttctatta agccagttct ggctcttcct ctatatcttc gtacttaccg
     2581 actcctttag ccttcttttt gcgttcaaga tgtaagtctt taagtaggca gtagatcata
     2641 aagaacatta tgatcataaa tggaaatcca ctaacaacag aagcagtctg taaagctgat
     2701 agaccaccgg taaagattaa tatcgctgct attgccccct cgcttactcc ccagaatact
     2761 cttaaaggac gaggtggatt tggatttcca cctgaagtca gcataccatt tacatatgta
     2821 gctgagtcag atgatgttat aaagaagagt gtaactgata gtgttccgag gaatattagg
     2881 aatttggcaa gcggcagcgt tgctaaggct gcataaaatc cggcagcttc actttgttcc
     2941 accgcttcaa tgaggccttg tcccccttca cctagtgcaa catgaagtcc tgtacctcca
     3001 aatacagcaa accatattaa acttactaat acaggtacaa acaaggaacc aataacaaat
     3061 tctctaattg ttctacctct ggaaacacgg gcaataaagg ttccaacaaa gggtgcccat
     3121 gcgatccacc aggcccagta gaaaatagtc caggccggta accaggcatt gtccataatt
     3181 gcatctgaca taaaggacat ttccacaatg tgctgcaagt attgccccag tgaattagta
     3241 aataatctta gaataaagag agtaggtccg attagtacaa caaatatcat taagatagac
     3301 attatccata cattaacttt actaagatat ctaatacctc tatctatacc ggttatggtt
     3361 gatatcacag ctgctgctgt aaccacaatg acaacaacga tctgggctcc tgttgaggcg
     3421 gaagcaatcc agtttaactg tgctaatccg gctacgattg acatcgcacc taagccaaga
     3481 gaagtagcca gtccaaagag ggtagcataa acaccgatta tattaatcag tctaccccaa
     3541 cctgtatcta ggttttcttt tcctatgata ggttctaaag ttgaacttaa taacattggt
     3601 aatcctttgt ggtaggaaaa gtatgctaaa gaaccaccaa ctatgatgta aattgaccag
     3661 ggatgtatac cccagtggaa aaaggaaagt ctcattgcat taatagctgc ctgttgggtt
     3721 tcagcagttg catttgctgc tgcacccgtg gcaggccaga tataatgcca gatcggttct
     3781 gatacactcc aaaatacaag tccaattccc atcccagcac taaataacat agaaaaccat
     3841 tcaaaacgcg tatactttgg ttcttcatct ggatctccta attttatttc accaattttg
     3901 ctaatcataa tataaatggc tactaataca aaaagagtac cacccaacat aaaagaccat
     3961 ccaagctgtt gggttacaaa gttaaaggag acatcagcag ctactctaag actatcaata
     4021 ccaaatactc cccacaatac aaataaaatg gtcaagataa aggcaatcca aaaagtctta
     4081 ttttctgacc aggatttacg caataatatc actccttata atttgaccca ctactcgact
     4141 aatcgttttt tactaattaa tcaaataaga aagtattaaa tgaatgtctt cacccctttg
     4201 ttttcaaatt ttgaactgta ttttttaacc acccccttat atattattat ttttaaccat
     4261 attacaaata tatacaattt tcttaatatt aagattatta atatttaaag aaaatatcga
     4321 aattgaatat ttcgattttc aactttttaa taatgctttg ttatcgatga ttgaacttat
     4381 gtatttcata aaagtggctg ttgcctgttt gggatcatat tcaaggcggt tattaaccat
     4441 tagggaaagc ataccatggt aaataagcct tatcctctca tctacttctt ctgctacttc
     4501 tgtggtgaag taacctgctt ctatacatgg tttgatatga attgaacacc ttttggaaag
     4561 ttcggattct aataacatgg gaatatatct tttgggaata ttatctaact cttctgggaa
     4621 aagctgataa tattttttaa ataagttttc agaactatct cctatattct cggtaaagat
     4681 tgcgtaaaag atatttggat tttcaaaaga gtttttagaa aaacactccc atattaatag
     4741 gtacttatct aattcatcct cacttttatt aagataaaat ggaatagctt ttacataatc
     4801 atctaaaaaa ctgatcgaac caaagaatat tagttggttt aggttatcaa agtaattata
     4861 gattgaggca ctgttatatc ctgctacttt agcgattttt ctaattgtga cattctcaat
     4921 accctcttca tttatcacct tccttacaga attaataaat gctcgcaaga tcctcttctt
     4981 cttaatgtca tcttgactaa actgtatcat ataatcactt ctccatttta gggagtcaaa
     5041 tatttaattt tagcctgtct aaaaagttaa tttattatta atcattttta gtattttaaa
     5101 catgtttaat tattaaccac gttttcctat ttctttataa taaataaaat tccttctttt
     5161 ttatacaagt tattattatc acaattaaaa taatcatctt gaaaactaaa cgtgtttgtt
     5221 atattattac attgttaaga ataatataaa aaataacata ttaaataaat tacttatttt
     5281 attattcttc tagtataaat aatagagggt gagtgtaaaa atatgataga tttcgacaaa
     5341 gatgacgtaa aagtaaaacg gattataaaa ttgtttgtaa gttctacgat tgatattatt
     5401 gaagaaagtg gtttagaaca ggtcacggtg cgtgaagtag ctaaaatctc tggatataat
     5461 ccggccacta tctataatta ttttgataac agccggcagc tgatcttctt tgcttcctta
     5521 aactttatga aagattactt taagggcatt gaaaaaataa ttgaacaaga tcaagatccc
     5581 ttagataatt acttaaacct atggagggag ttttgtcgtt actcctatca aaatcccaaa
     5641 atttattatt caatatttgg ggaaaatatt gatgaaagtc ccaaggtctt aatcgaaaaa
     5701 tattttaaac tgtatccaga tgactttaaa tattcaccag ccgattacct accggttcta
     5761 actgactctt tcgccaagga taatgtaact aaaccattaa gaatatgtgt gaacaaaggc
     5821 tgtttcacgc tcaagcaggc caaaaagatc gatgaagtaa atatgttttt ttatcagggt
     5881 atgctttctt tacttgtcaa tcaaagggtc tcctactctg ttgaaaaggc aactaaaaaa
     5941 actgtggacc acataaaaca ggtcattaat aatgtagtgc ttgaaaacag tgaaaagaga
     6001 ttagaagaaa ttgactttta aaagaaatat tcttaaaatt taaggaggat ttattgtgag
     6061 ggaaatttat cttgataatg ctgctacaac ccgccacaag ccgcagaccg tgatcaatgc
     6121 cgtccaggaa ttcatgacag aacttaactg tagtcccggg cggggtggtt atgaatgtgc
     6181 aatacaggcc ggtcgtcaga taatggatac cagatttctt atttccgatt tctttaacgc
     6241 cggtgaagtt gaaagcattc tatttactaa aaatgttaca gaatctttaa atatattgat
     6301 caagggatta ttaaaagatg gtgaccatgt tattaccact tcacttgaac ataatgcagt
     6361 gcttagacct cttaatcaac tagaggaaaa agatatgatt gaggtcagtt atgttgaagc
     6421 tgatcaagaa ggaatcttag acccttcctt aattgaagat gcggttaagg ataatactaa
     6481 actgattatc ctaaaccatg cctccaatgt aactggtact atcttaccgg cggccgaagt
     6541 tggtgaaatc gccaaaaagc atgatatttt ctatgctttg gataccgctc aaaccgctgg
     6601 tgtacttgat attgactttg aaaaattaaa tttagatttt ctcggattta ccggtcataa
     6661 aagtcttttg ggtccccagg gtaccggtgg gatggcgatc agcgaacgat tagctgatga
     6721 gatggaaacc ctgattgccg gtggtaccgg tagtttatcg gaaacggtta aacaacccga
     6781 ttttttaccc gataagtttg aaagcggtac ccttaataca ccgggcattg tcggtctaaa
     6841 agccggtatt gaatatatca atgaggttgg gattaaatct attcacaacc atgaaataag
     6901 attactggaa agatttttag aaggtttgaa gaacctggat aaggttacgg tttacggttc
     6961 aaccgattta gataaaaaag tgcccactgt acctttaagg gtgaaaggtt atgatctggg
     7021 agatttaggt tttaaattag ctgagaagtt tggaatcatg gtcaggtctg gactacactg
     7081 tgcccctctt gcccataaga cgattggtac ttttcccgat ggtgctttaa ggttctcgat
     7141 tggttggcac acaactgaag aagatattga ctatgcttta gaaaccttag ctaaattata
     7201 aggagcttaa cataaataat ttaataaata aatccgggtg attttatgga aaataagctg
     7261 aatctaaaag aaaaaggaac tattgtattt aaaagattag ttaaaagata tataaaccat
     7321 gatatttcag cttatgcggc cttgatggct ttctttttaa cactttccct ttttcctttt
     7381 ttgattattc tctttgctgt tttaggtcag ctatctttag atacaaactg gattattagc
     7441 gcgttagaac ttttcttccc gagtgaggtc cacggacttg tcatggaatt cataagacag
     7501 aacattatcc tcaaggactt tagtgttctg tctttatcag ttgtaggtgc tctttgggct
     7561 tcctcccgcg ggataagagc cttgatgtta tctttaaata tggcctatga agttaaagaa
     7621 actagaaatt attttgttgt aaaactaatt gatatattat atactcttct cattattatt
     7681 ggtattgttt ttctgcttac cttacccaat atagggagag aatttttaag atatattgat
     7741 acttatatta caatcgattc ccgcctaata gaattgatag ggatctttaa actgactgcc
     7801 ctaccggtaa caatcgttct tattatttca actttatata tgcttttacc caataaaaag
     7861 cagcctttta aaaaggtagt gccgggcaca atatttacgg taataggctg gactatttta
     7921 tcatacctat tctcactttt tgttaactat atagctaact attccttaat atatggttcg
     7981 ctttcaacaa ttattatctt aatgctctgg ctttattttt ccagtatcat tcttatcctg
     8041 gggggagaat taaattcggt ggtaaaagaa atttaactct tattataatt taaattatta
     8101 aggagcaatt attatgagtg atatggttaa acacgtaatc gttggtgata tgttaggtac
     8161 cctttcagct tactttaata aacctataat taataatagt gtctttgcct ttggactcgg
     8221 ttttatcagt catgctgtct tggactatac agaaaatgat tatgtggtta actggtttaa
     8281 taaaagtgaa ttcacagctg ccttaccctt ccttattttt caaatcactg taacagctat
     8341 cttactatgg gtttttattt ataataataa aaaatacagc agtgagtata ccagactaag
     8401 aataatcgca gttatcgggg ctatttctcc tgatctaatc gatgccctct actctaccat
     8461 taatcccgcc gcctggtaca acggccagtt gattttcccc tggcattccc tgcaggcggg
     8521 ctctgataag tttatcatgt ccatgtatac cacgatggct attacggttg tttttattat
     8581 aacaaggtat ttattttaca agtatctatt taccaaggaa ctgatctatc cggaagctaa
     8641 agaacaccat ctacaagaaa ctccggttga agatagttat cctaattaaa aaatgaataa
     8701 ttagtcgatt cccagtctgt tttattgact gggttttttt atttttacct ttctccactt
     8761 ctgattatct atcttatttt aattcatcat tctataattt ttactattat gctattttca
     8821 agctttataa tttgcaaaat ctttacattt tctttatagc aaattttact ttaactgtta
     8881 cgatatatat gtaaaggagt ggagaaaaaa tgaaaatatt aaactatggt attgatatat
     8941 ttttaatagg ctatatgatc tactttctcc tattcgtatt tctcggtctt aggacaaaaa
     9001 agaataatga cttaaaagaa ccggccaata ggtttgcggt agtcataccg gcccataatg
     9061 aagaaaaggt aatagataaa ttgattgaaa acttacgcaa cttaaattat ccgaaagatc
     9121 tttatgacat atatgtggt
//
LOCUS       DATGCJ010000053        40955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_319562, whole genome shotgun sequence.
ACCESSION   DATGCJ010000053 DATGCJ010000000
VERSION     DATGCJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 40955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40955
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319562"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VKN64_02470"
     CDS             complement(<1..851)
                     /locus_tag="VKN64_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMA59129.1"
                     /translation="MKKSKLFLTFFILLFLIANTVTISAEEMSLDKAVQYFLNNSNQL
                     ENAKRDIQASQLDLKIAEKGLAPEVTLQTSYTRLGEAPQSPSEYILRPTGQTTFFGQP
                     ATKYQFIPVSFEDQPQDNYNTTLTVNKPLYLGGQVRNSVNLSEKAVQLNNLKYEQTLN
                     DNLNAVIQSYFNVLIKESLVEIQKNSLKLVESHLDSVQKNYEAGLVVKSDVNEVKIEL
                     NKTKQSILTAENDLKIAKKRLADLLELDNTDFTLKQPDIPALEEKLQTQLDKAYANRF
                     EMKSLVI"
     gene            complement(838..1431)
                     /locus_tag="VKN64_02475"
     CDS             complement(838..1431)
                     /locus_tag="VKN64_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59130.1"
                     /translation="MKEDTRTTIMETAIKLFADKGFSDTSMSKIANQADVGKGTIYWH
                     FESKEDLFLSIIEEKGKSYFNQVLKLGKSDMKPADIIYKYIRDRIEFIENNYDVARML
                     ISNTDVINRDFKDTMEKRHKQMVQVLENTIQKGIEKGTFRAGKSDEYALMIINTTNSA
                     HNNFSCGIKGDIETRTKKIYDFIMHGLDRREPDNEKK"
     gene            complement(1661..2437)
                     /locus_tag="VKN64_02480"
     CDS             complement(1661..2437)
                     /locus_tag="VKN64_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMA59131.1"
                     /translation="MKNIKVLSLTLLIILSLLIVSILNENAYSQVPQNPLKLVAIGNS
                     TPAGYGIKPQENYLRVYAEYIEQDLNVEVEIINWAKLESRNLLGWVQAITTDEVFRMD
                     IKNADIITIWISWHEIMPYIYKTERVYKKELNMTTKKMGDSFNILFAEITRLADPKET
                     LIMVAETGIPPAIAETWKENNFYQELKQYAYEDWREYLIEAAENNNIKVVPTYKAFNG
                     QAGNSMLPEVYIQKDGIHFSAKGHKLIADLHRNMGYNPLK"
     gene            complement(2663..3268)
                     /locus_tag="VKN64_02485"
     CDS             complement(2663..3268)
                     /locus_tag="VKN64_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59132.1"
                     /translation="MKKVIVSTLVLSLIVLAFGAFAVEAQMGNKMGGAGNRIDNNIER
                     PAFQQNAQSEVEVLDLDEEQAEKVAELKEKHFAQKDQFVEKLQSTQRELREEVLKNES
                     QEKITALETEVEKLRAQIDQARTDYLKEIKEVLTEEQLQKIAENEYRMGLAAGNGNKI
                     ASSNTYNKYGRNQVDSSQRGQGRGRHSAGRRGGYNNITQNQ"
     gene            complement(3460..4749)
                     /gene="eno"
                     /locus_tag="VKN64_02490"
     CDS             complement(3460..4749)
                     /gene="eno"
                     /locus_tag="VKN64_02490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMA59133.1"
                     /translation="MISPFIEDVYARQILDSRGNPTIEVDVVLEDGTLGRAKVPSGAS
                     TGAHEAVELRDGGDDYMGKGVLDAVKNVNTIIGPELIGRDVRDQILIDNLMIEMDDTD
                     NKGRLGANAILGVSLAVARAAASASDDFLFNYIGGVSANLLPVPMMNILNGGEHADNN
                     VDIQEFMIMPTGAESFKEALQMGAEVYHNLKKVLQSRDLGTGVGDEGGFAPNLSSNEE
                     ALDVIIEAIEKAGYEAGEDIMLALDVAATEIFADGKYNFKGEGVMRTPEEMVDFYEGL
                     IDKYPIISIEDGLDEDDWDNWALLNERIGDRVQIVGDDLYVTNSKRLKKGIENDVSNS
                     ILIKVNQIGTLTETLETIEMAKKAGYTAVVSHRSGETSDTTISDLVVAMNTGQIKTGA
                     PARSERVAKYNQLLRIEEALGSSAKYAGMNAFYNINK"
     gene            complement(4768..6306)
                     /gene="gpmI"
                     /locus_tag="VKN64_02495"
     CDS             complement(4768..6306)
                     /gene="gpmI"
                     /locus_tag="VKN64_02495"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMA59134.1"
                     /translation="MNRPRPLALIILDGYGESEQIEKNAVKNADTPFLDKLREENPTS
                     IIQASGEHVGLPPGQMGNSEVGHLNLGAGRIVPQEYTRITKAVEDGSILENDVIQKAI
                     QKVNDNNSALHLMGLLSDGGVHSHINHLFGLLEMAKTAGLDNVYVHAILDGRDTPPQS
                     ALTYIEQLEEKMEEIGLGKIATISGRYYAMDRDNRWDRTKKAYDAYVKGEGIEVSSAE
                     EAVKKSYERGDNDEFVIPSVVIEDGEPVTTVKDEDALIFFNFRADRARQITRALGLDG
                     FDKFERPNKHPNGLYFVCMTEYDEDFDLPIAFPKLHIDNILGKVLSDHNMRQLRIAET
                     EKYAHVTFFFNGGEEREFEGEDRELIPSPKVATYDLQPEMSAFKVKDRLLEKLDEDIY
                     DVIILNFANPDMVGHTGFYDAAVEALEAIDQCMSEIVPKIEEMGGQMLITADHGNSEQ
                     MIDEKGEPHTAHTSNPVIMMYVGSEEKVNLKDGRLADIAPTMLDILDIDKPDEMTGNS
                     LIEK"
     gene            complement(6347..7105)
                     /gene="tpiA"
                     /locus_tag="VKN64_02500"
     CDS             complement(6347..7105)
                     /gene="tpiA"
                     /locus_tag="VKN64_02500"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMA59135.1"
                     /translation="MRKPFIAGNWKMNKTISEAKDLVSSLKEKVADIENVEVGVCPPA
                     IDLQAVKKVAAGSNVKVGAQNMHWEESGAFTGDISPVMIKELGLEYIIIGHSERREYY
                     NETDEQVNKKVHAAFEHGIKPIICVGESLEERKANETRDKVEKQVKAALKGLNQKQVS
                     QTVIAYEPIWAIGTGESATAKQANQVISYIRDVIEEEYLDAADDVRIQYGGSVKPYNI
                     EEFMAESDIDGALVGGASLEAESFAAIVRKTSEM"
     gene            complement(7122..8306)
                     /locus_tag="VKN64_02505"
     CDS             complement(7122..8306)
                     /locus_tag="VKN64_02505"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMA59136.1"
                     /translation="MKKSLKDYDFKGKRVLVRVDFNVPLEDGKVTDTNRIEAALPTIK
                     YLMNEDAKVILMSHLGRPKGEVKEEFKMDPVGKELANLLNREVVKVDDCIGDEVKKAV
                     SNMENGDVLLLENTRFYPGEKANDPEFAKKLAENADIFVLDAFGAAHRAHASTVGVTE
                     YLPSAAGFLLMRELNALGDVMDNPARPFVAIMGGAKISGKISVIENLLDKVDYILTGG
                     GIANTFLKSQGYEVGESLVEDDKLETARELIKKAKEKNVILLTPKNVVIADKFAKDAN
                     SKEVDVGDIPEDWQILDSGGSKTIEEYTKIIKKAKTVIWNGPIGVFEFEKFAKGTTAI
                     AKALAETDAKTVIGGGESAAAVKQADLIDEMTHVSTGGGASLRFFEGKPLPAVEAIDD
                     LK"
     gene            complement(8388..9401)
                     /gene="gap"
                     /locus_tag="VKN64_02510"
     CDS             complement(8388..9401)
                     /gene="gap"
                     /locus_tag="VKN64_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMA59137.1"
                     /translation="MSVKVAINGFGAIGRKAFRGGVKNPDIDFVAINDLVEPKILAYL
                     LKYDSNHGTYDGEIDHTDNSIIVDGKEIKVYNKKDPAELPWGELGVEVVYEATGLFTD
                     GEDAKKHLEAGAKKVIISAPATNVDKSFVMGVNDDEYDPDNHNIISNASCTTNCLSPI
                     AKILNDEFGIEKGLMTTIHSYTSSQNILDGPYKWKKITRGRAAAENIVPTTTGAAVAT
                     TKVLPELKGRLDGMAVRTQTPTGSLVDLVVTLKEDVTVEDINRVMKEYAEGDMKGILE
                     YNEDPIVSRDIIGNPHSSIYDKNHTKVIQGNMVKLLSWYDNEWGYAQRLNDFAIKMKE
                     LGI"
     gene            complement(9468..10517)
                     /locus_tag="VKN64_02515"
     CDS             complement(9468..10517)
                     /locus_tag="VKN64_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HMA59138.1"
                     /translation="MNYLLKIQKKLVPELISIAEIRYNILKEVFNHQPIGRRNLARKL
                     DLSIRNVRKHLDFLYEKRYIKITKSGTILTENGEDFLHELDTYMKEIKDIANIENEIE
                     KLLGIDKVMIVPVEKDSEIINKEIGRFTARFLKEMINDQDIIAVTGGSTLAQVAETMV
                     FLEEPKDVTVIPGRGGLGENVEIQANTIASKIAKKLGGQYKLLHIPDDITRESLAMIT
                     NEPSVKKTLDELQKANILVHGIGTAEMMSARRDMEEAEIEKLKDKGAIAEAFGLYFNE
                     EGEVVYSTASVGLSMADLKNIDKVIAVAGGVDKARAIISVVSPEYHDILITDELTARE
                     IKALKGGEKEQKLNK"
     gene            complement(10640..11974)
                     /gene="rpoN"
                     /locus_tag="VKN64_02520"
     CDS             complement(10640..11974)
                     /gene="rpoN"
                     /locus_tag="VKN64_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMA59139.1"
                     /translation="MDLALDLSVDLEQKQELVMTPKLQMAIKLLQYSSMELKEYVEEE
                     MKDNPLLEKHDENENHLDDRIKNQYQSYRYGSNYNQEDFNYENMVTYQPNLLEYLENQ
                     LYEVLDNDETEAGEYILGNLDERGLLSTSLDEIAEELDEDIDYVKSIIKKIQKLEPAG
                     IAATNLKECYLTQLKQMDVKTNDAELLIGNHLDLIVTEEYKEILDKTGWSEDRLSKAL
                     HIINKLTSKPASLVADHKKTQYIMPDIVIKKVNDEYVIVLNDKASPLLRINPYYYEMM
                     QKCKKDDTYEYLEDKFKSALWIIKSIEQRRMTVYRIAKTIADKQKNFLDDGVKYLKPL
                     TMQEIADEIDMHESTVSRATDNKFIQTPRGIFDFKFFFSGGVNDISTVSIKAIIIEII
                     KEEDKSSPLSDNAIAELLRTKQNLKLSRRTIAKYRNELGIPSSVKRRKKYSI"
     gene            complement(11958..12908)
                     /gene="whiA"
                     /locus_tag="VKN64_02525"
     CDS             complement(11958..12908)
                     /gene="whiA"
                     /locus_tag="VKN64_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HMA59140.1"
                     /translation="MSFSDDVKHELAHLEIEQKGIHQAELAALIRMGGSILLSSGKMA
                     VKISVSHGDVARRIYRYIKEEYQFESEIRVKKNNLSRRKIYELFITPQKGVYDLLENL
                     GILNKSHSLLFTVADWIKDKRDYKRAYLRGVFLAGGSVNKPGAEYHLEIRCEHKSHAE
                     EIIDLMQDFELDPHLTEHKKKQVIYLKRYDDIATMLNLMGAYNSLLKLENTQVFKEIK
                     NNVNRRVNFETANLDKTVKAAMEQIEDIELIEEIKGLDNITESLREIAELRKDNPYAS
                     LKELGEMLEPKLTKSGVYNRIRRLKKIAEKIRSEKNGSSS"
     gene            complement(12931..14196)
                     /locus_tag="VKN64_02530"
     CDS             complement(12931..14196)
                     /locus_tag="VKN64_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HMA59141.1"
                     /translation="MKFLKWLYPGLGVKRWFILGIIGLLLLSTGLALLLHINIVDYII
                     KMIFEAITHLSGSLPYYTNLIIGIALIIISLAMLTYAGNRFVDKLNKEIYKDDDFVNV
                     LYKRNQLQKGPKVVAIGGGTGLSNILRGLKEFTSNITAIVTVADNGGSSGKLRDELDI
                     LPPGDIRNCLVALADQEPLMEDLFQYRFHKKGDLRGHSFGNLFIAAMTEVLGDFEEAV
                     EESSKVLAIQGKVLPSTNQNVNLGAVYEDGSEILGESEIPDENKVIQGVYLEPSSVEI
                     TERAKSALLDADYIIVGPGSLYTSLLPNLLVKGMVDTIRESKAKKIFITNVMTQPGET
                     TGYSAYDHLKTVYNHIGAEIFDYTIINKNGSRKDLLDKYAEEGAYPVENDIEQLNKLD
                     IKVVEGKLIQAGDYIRHDPHKLAEILVEL"
     gene            complement(14211..15062)
                     /gene="rapZ"
                     /locus_tag="VKN64_02535"
     CDS             complement(14211..15062)
                     /gene="rapZ"
                     /locus_tag="VKN64_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HMA59142.1"
                     /translation="MKFILITGMSGAGKSTTLNFFEDSGYFCVDNLPPALISKFAELC
                     LHSELNKIAVVCDIRGGKFFDALTEKLNYLEEHQINHEILFLEASDKALVDRYKETRR
                     RHPLEEEGRVLDAIREERRRLEQLRGKAHNVIDTSSISIKELKQKLRRDYLDVDEDMQ
                     SLTINIISFGFKYGIPLDADNVFDVRFMANPYYVDSLKDLTGEDKEVKDYILKWPITK
                     RFYDKFFDMIEFLIPEYEKEGKSHLSIAIGCTGGHHRSVTTAIELKDFLDSKGYKVTV
                     NHRDINK"
     gene            complement(15131..16087)
                     /locus_tag="VKN64_02540"
     CDS             complement(15131..16087)
                     /locus_tag="VKN64_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMA59143.1"
                     /translation="MQANSIKEFNPQQNKIFTIINPVSSNGKTGKRWPAYEQKFKKAK
                     LNLDINETLYPGHAVNLTKTALNDGYKTIMAVGGDGTVNEIVNGFYQDGQLINQEAVL
                     LVFSQGTGSDYIKSLGLNKSIETVINTYKKYERKWVDIGKMKYLNFDEKEVERYFVNE
                     ADVGIGGETVEYVNRSSKLMGGLLTYLIGAIRTVVKYDNKDMKLTIDGEVIKEGLINS
                     VMVSLGKYFAGGMPIAPQAVIDDGLFDIVILGNLTKLETLLNLYKAYRGTHIDYHKID
                     YLKGKEIKIDSKDRVLIDIDGESAGLLPAEFKILKKAFPVLV"
     gene            complement(16182..17171)
                     /locus_tag="VKN64_02545"
     CDS             complement(16182..17171)
                     /locus_tag="VKN64_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HMA59144.1"
                     /translation="MLKNKNKKALDFCRSIMQKVSRSFALTIPLLDKRIREPVMITYL
                     QDRLLDSFEDEGEINSSQRKAYMNEVVKLFDPNISESRQQELVANIKGQADYFEGPIK
                     KLVQNTDKLISCFNTLNEEVQKISFRWLSEMNQGMQSYLDKPVETFSELNEYCYYVAG
                     TVGGFLTDLIIELGNIEGKDKKVLEKNFVESGLFLQKVNIIRDIKLDIINHKKHYWPL
                     KELGISESEIIDQNNEEQALKALNKMVADVKSHTRALIDYYQAIPEEWSGYKKFYSVN
                     NAMGYATLELVEGNPDLFYLNKKLKIKKTEVLKILAVSEDTFLKKAQSVLNQG"
     gene            complement(17140..18090)
                     /locus_tag="VKN64_02550"
     CDS             complement(17140..18090)
                     /locus_tag="VKN64_02550"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HMA59145.1"
                     /translation="MSSGHKYVLTISLIIFITTFYAAAQAEDNLIFFKVDPAGDDHGP
                     GYYLYPQHEVFQKEEGLFDIVEFKIYESSSQYEFQFEFTKLVDVWNSRFGFSHPMVQI
                     YIDNEKGGSIALFEEGARVKLNPEYPWDKLLKINGWWVSLYEPLDRNRQVVDFTVDGE
                     DVPWVINNPELKVQDNWIKLKIAKEKIGPLQGANLFILVGGFDPFGYDHFRGIRDELN
                     SWFFAVQGDHDINYAPRVIDLIVPPEYSQEKMLDAYEIGYAQIEPVYIGHDKEREKQD
                     YYGYTIIFLILSVLIFISIKKIQKRLGVNGNAEKQEQKGT"
     gene            complement(18114..19094)
                     /locus_tag="VKN64_02555"
     CDS             complement(18114..19094)
                     /locus_tag="VKN64_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMA59146.1"
                     /translation="MSKDYYIGIDIGGTKVLSALADSEGKIINKAQNPTEADKGEDVI
                     ISNIEDSIERVIENSKISKKEVKRIGVGSPGPLDLNKGVIIENSNLAWTNVPIVEILE
                     EITGIEVILENDANAAALGENQFGAGQAADHMVYLTISTGIGGGIIINRKIFHGGKGN
                     AGEVGHMTLIPDSDYQCGCGNYGCFEAVASGTAIARRGRESLTVHQDSLIYEYSEGNP
                     DVVDAPLIAAAAREGDQEAIRIFKLTGQLLGVGIANVVNLFDPEIILLGGGVMHAQDL
                     FMQELKASLKERALKPNLKDLQLKEAKLGKDTGVKGAIAVAMCDYLYQNQ"
     gene            complement(19087..19785)
                     /locus_tag="VKN64_02560"
     CDS             complement(19087..19785)
                     /locus_tag="VKN64_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59147.1"
                     /translation="MKIWQVLKDSTSQTYDKLFKFAILNLIWFMLSTLVVFIGYSGLI
                     IKFYYLLIVPIIFLGPFFMGGLMSGINYIEFNDFSIKQYFIYIKDNFKRGLLGFLFTF
                     FIYLLLIADLVFFLQKGTDSLFMLVLAVLVLYILIFFSMMQCYYWGFLALDKEKKIRY
                     VIKNSFLVTIDNVVFSFLWFIIVFIISAILIVTGFGMAVLLMNFLSLMMLNGTIAVSS
                     EYDNLEIEEMNRDE"
     gene            complement(19893..21287)
                     /locus_tag="VKN64_02565"
     CDS             complement(19893..21287)
                     /locus_tag="VKN64_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA59148.1"
                     /translation="MATFNFAKKFAADKLVKKGLEYIEKDPEENFLKILNLAEKLSNT
                     KNQDRQIEVIRENYKKNPVIKEFIRKLDDVAPSYKEGLLINFFVNSALLGIPYQYKMA
                     DKLGHGVPWAILMDPTSACNLNCEGCWAGEYDKSDTLEFETMDRIINEAKELGIYFII
                     FSGGEPTVYPRLMELVERHPDVGFMMYTNGTLIDDEMADKMLELGNITPAISLEGYKE
                     QTDARRGEGVFDKIMAAMDRLRERGIIFGVSLTAHRNNVYDLFQGEDFYDFLIEKGAT
                     YGWLFHYMPIGRDPDLDMMLTPEQRGFLAERVNELRSNKPLFLIDFWNDGTYAGGCIA
                     GGRRYFHINAKGDVEPCAFAHFATDNIKDKSLKEVLDNPLFREYQKRQPFNENSLRPC
                     PIIDNNPALREIVKESGAEPTHAGAGNVITEEVGKKLEDLAERWEKVSRPIHEKRVSK
                     KEKEKEKKKEKVKS"
     gene            complement(21373..22170)
                     /locus_tag="VKN64_02570"
     CDS             complement(21373..22170)
                     /locus_tag="VKN64_02570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HMA59149.1"
                     /translation="MYKLIVIDLDNTLLNNEHRISAKNKEVIKQVQAKGIEVLIATGR
                     MYISAEPYIKELSLDNEAIVYNGAMVKEIKSGQIKYHKPIKKDIARKIIRDVKNEGLH
                     INLYQNDQLYVDQDNEYKKRYEEVSGIKALQVDNLAEMDFDDPTKLLIIEDDPDKHSY
                     YQKYLRDKYGEQIDVTESKHYFIELGAKGVNKGKTLQQISQVKQIEQKEILAIGDSYN
                     DIEMISYAGTGIAMDNAAEAVKEKADLIAPHHEEDGVAEILDKLILS"
     gene            complement(22285..23514)
                     /gene="glyA"
                     /locus_tag="VKN64_02575"
     CDS             complement(22285..23514)
                     /gene="glyA"
                     /locus_tag="VKN64_02575"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMA59150.1"
                     /translation="MSSLKEVDPKIAEFIELEKERQAKHIELIASENFVSDAVLEAAG
                     SKLTNKYAEGYPHKRYYGGCEQVDKVEDLAIERAKKIFGADHANVQPHSGSQANQAVY
                     FAKVPIGETILAMDLTHGGHLTHGNPVNMSGKYFNFIHYGVTKDREIIDYDQVEKLAK
                     KHEPTLIVAGASAYPRVINFKRFKQIADEVGALLMVDMAHIAGLVAAGYHPNPVEHAD
                     IVTTTTHKTLRGTRGGLILCKEEHAKDIDKAIFPGIQGGPLMHIIAAKAVGFKEAMTD
                     GFKEYQKQIIDNAQALAKSLQSFGLRLVSGGTDNHLLLVDLNNTDITGKEAETALDKV
                     GITVNKNTIPFETRSPFVTSGIRIGTPAVTTRGMEEEEMELIGEYIFECLKYIDDDEK
                     LKDLQKKVYELSAEFSG"
     gene            complement(23722..23892)
                     /locus_tag="VKN64_02580"
     CDS             complement(23722..23892)
                     /locus_tag="VKN64_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA59151.1"
                     /translation="MAYVISDECIMCGACEPECPVDAISEGDTQYVIDADLCIDCGAC
                     AEVCPVDAISPE"
     gene            complement(23984..24298)
                     /locus_tag="VKN64_02585"
     CDS             complement(23984..24298)
                     /locus_tag="VKN64_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59152.1"
                     /translation="MSLRLEEVNKVDKLIFLKFNEVNILIYCNSKEGIEPIFTEIYKL
                     ENKAVMFNEILDLLDNHVKKKVVLNKSDLEIIQKNANNDLKFEALFEDEQKISKYILI
                     ST"
     gene            complement(24282..25736)
                     /locus_tag="VKN64_02590"
     CDS             complement(24282..25736)
                     /locus_tag="VKN64_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HMA59153.1"
                     /translation="MAANPISEVVKIKRKIYIELARQVLEGTIEDKIQSIPYKIVRKK
                     DERYRCCIHKERAIVKERIKLALGFDPQLHKQEAPADLLTKRGEEDCCANTVNVIELA
                     CDSCPIDKYTVTDSCRNCVAHKCVNVCPVDAIITIQNKAFIDQKKCIECGRCKKACPY
                     GAINENIRPCVRACSVDALSSNSERKAVINYNKCIDCGSCIQACPFGAISDSTELVKI
                     CRALKDKDDKLTALLAPSFVGQFGAKVEPATIKSALKEIGFDKVIEVGLGADLTAVEE
                     KKELSEKLNKEEFLLSSCCPSFKKLVINEYKELIKNISHTFSPMVKTAQRVKKESENK
                     TVFIGPCIAKKAEACLYTSVNHVLTFEELLCIMVAADLNLAAYCDQEAVQDATKHGRQ
                     FAYSGGLSEVMQELTEAKLETVNGNGLEQCIEVLDKVLEGESEFDFMEGMACHGGCAG
                     GPSVINKPQVTKKLVQLFAEEANLKKAEYNEFTA"
     gene            complement(25764..26000)
                     /locus_tag="VKN64_02595"
     CDS             complement(25764..26000)
                     /locus_tag="VKN64_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HMA59154.1"
                     /translation="MDKITIEVCVGTPCYLMGARELLDDMQKLESESEGKIKVKTSHC
                     IDDRCEKAPVVRVNGEVYQDITPDKLRKIVSAKL"
     gene            complement(26018..27169)
                     /locus_tag="VKN64_02600"
     CDS             complement(26018..27169)
                     /locus_tag="VKN64_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMA59155.1"
                     /translation="MGIVIDEFYQSIPKRGQEVCGDNVEIIKNDKETIVVLSDGLGSG
                     IKANILSTLTSKIAVGLIDKKLPLIEIIETVLATLPICEVREIAYSTLAVLRVNRNGK
                     AQIIEIDTPSIFLVRNGQVKKIEMQEREMWGKTVREAEVKLENDDKLFMVSDGVIHAG
                     IGALLDFGLGWQGFARHLDDVLQHYYNLKDIVEKLVDICLAYYLMEPGDDFTVLGLGF
                     REERSLTMLSGPTQSPQKDEEVVFELLEGPGRKVVSGGTTASIVARELNQKVKSTFEY
                     HDKAVPPIMKIKGMDLVTEGLLTLNKTADYLANHKQGQLYPEGKDGAARLVRMLLASD
                     YIKMIVGRAMNESHQSLNLPLELGIRTQVMNRIAKSLKKLNKEVEISWY"
     gene            complement(27169..28878)
                     /locus_tag="VKN64_02605"
     CDS             complement(27169..28878)
                     /locus_tag="VKN64_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HMA59156.1"
                     /translation="MKVLDVVKSSCQDCHRCLRSCEVGAIGFDEQAWIIEDKCIYCGT
                     CVNICPQNSKKPYDMKGRLLKYIDNEEKVIVSLAPSYLTAYQDIEWYNIIGTLRAMGV
                     DMITETAQAAELVADYFNKEIKDRKKPLISSCCPAVINLIEKHYPEMREYLADIVSPM
                     NLHASLLKTKYGEKSRVVFIGPCIAKMDEMESDRTESKVDAVITFYQFKKLCEENGIE
                     LNDHCSYQIEEGCGEISGTYPLENGAVRASNLLSDYSQNEIISGNGVQNSQRILTDLK
                     EKKIAPRFIELMACEGGCINGPGVENAASTVSKEIKVSQYAEKKDVKYNSHSLEKYLA
                     EVDIKRNYKNRQKEYEEPTEAVIRSILKEIGKETPEDETNCGGCGYDSCREKAIAVYH
                     GLAQKNMCIPYMKSKAESLSHIIVESSHNAIIVVDKEMVIQEFNPVADEMFGEHKVTE
                     NGQKLADYIDPHLFKEVWQTKDSIVHKKVEYEQYDLITEQTIFPIEEYKVIVGIFSDI
                     TERERQKKRVQEMKELAAEKAEDVVHQQMKIVQEIAGLLGETTVETKSALHELTQLMQ
                     EEK"
     gene            complement(28865..29125)
                     /locus_tag="VKN64_02610"
     CDS             complement(28865..29125)
                     /locus_tag="VKN64_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HMA59157.1"
                     /translation="MLEIKICVGSSCHLKGAPEVVEEFKRLIGEKELEDKVNLKASFC
                     QNNCTQGVNITIGSQKVANVAPEDVEGIINKKILGEIVNEST"
     gene            complement(29208..31034)
                     /gene="uvrC"
                     /locus_tag="VKN64_02615"
     CDS             complement(29208..31034)
                     /gene="uvrC"
                     /locus_tag="VKN64_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMA59158.1"
                     /translation="MSEYIEEQLKQIPDKPGVYLMKDDSGEIIYVGKARSLRKRVRSY
                     FRRGNQSYKTSIMIDHIDDFDYIVTDTEMESYILEANLIKKYHPKYNVRLKDDKTYPF
                     IKITTYEDFPRIKKSRVVKKDGSKYFGPFADVDAIYKTINILKDLFKLRSCNYDFSVD
                     NQLERPCLNYYIDKCTAPCVGKISKEEYRAQIDQVIMFLAGRQKTLLNKIEEEMEAAA
                     DEQNFERAARYRDAMQAVEEISRQQKVMSEDNKDRDIIAITEDGSYCVQLLLVKNGKL
                     IGQEHFILKGTRGEDEREVMSSFLQQYYEQAPQLPDEILINTEINNREILADRLKQLK
                     GKQVKIKIPQKGNKKRLIEMALKNAKQNLKKESIKKKYEEKRTVKAVEKLADYLELDE
                     LPSHIEGVDISNIQGTDPVASLVVFKNGRPSKSDYRRFKIRQKEGPDDFAMMKEVVHR
                     RYRRLLEEDRKLPDLILIDGGKGQLNAALTVLEELGLADMQIIGLAKREEEVFVPNRK
                     EPIILPKSSDALHLLQRVRDEAHRFAVNYHRKLRSRRITHSMLERIPGIGPKKKKALL
                     QHFGSLAKIRLADRSELKEVEGISDKLAEVISDYLEKNTRSM"
     gene            complement(31048..33861)
                     /gene="uvrA"
                     /locus_tag="VKN64_02620"
     CDS             complement(31048..33861)
                     /gene="uvrA"
                     /locus_tag="VKN64_02620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMA59159.1"
                     /translation="MGSKKITVKGAREHNLKNLDVDIPRDELVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPKVEYIEGLSPAISIDQKSTSKNPRSTVGT
                     VTEIYDYLRLLYARIGTPHCPICGKEISSQTIDEIVDQVLELKNRTKLQILAPVIRGR
                     KGEHDQVFAQARRDGFIRVKVDGDNYLVDDEIDLDKNKKHDISVIVDRIVIKEDIRER
                     LADSVETALEYADGLVYVDIIEGETLTFSEKFACNEHGFNLEELSPRMFSFNSPYGAC
                     EVCDGLGIKKEFDPELILDKERSIQDGGIIPWRNSSSKYYPQILKALAKEYGFSMSTP
                     IGELDEEIQDILLYGSDRTLSFPYTNRYGKTRQQNTQFKGVVNYLKRRMKRSDSASSH
                     SRMEKYMREDDCKACNGQRLKPGALAVTVGGISIAEFTRKNIGEALEFIKNLKLSKRE
                     KFIGQEILKEIRNRLQFMVNVGLEYLTLERSAATLSGGEAQRIRLATQIGSGLVGVLY
                     ILDEPSIGLHQRDNKRLIDTLEHIRDIGNTVIVVEHDEETIRTADHIIDIGPGAGKHG
                     GNIVVQGDLEKVKDCDKSLTGKYLKGEEKIPIPDERYEPNGKYLEIKGAKQFNLKDID
                     VEIPLGTFTCITGVSGSGKSTLINYTLKRRLMQHFYDSTEHPGKHDEVKGLEHLDKVI
                     NIDQSPIGRTPRSNPATYTKVFDYIRDLFAETPEARRRGYDKGRFSFNVKGGRCEACK
                     GQGKNKIEMHFLADVYVTCDVCGGKRYNKETLEIKYKGKTIADVLDMTVEEALNFFDK
                     IPALKRRLQTLYDVGLGYIKLGQPATTLSGGEAQRVKISEELGKRSTGRTLYMLDEPT
                     TGLHFADVKKLLEVLHRLREGGNTVIVIEHDLDVIKSADYIIDLGPEGGDNGGEVLAT
                     GTPEEVAEADNSYTGKFLKDILK"
     gene            complement(33872..35875)
                     /gene="uvrB"
                     /locus_tag="VKN64_02625"
     CDS             complement(33872..35875)
                     /gene="uvrB"
                     /locus_tag="VKN64_02625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMA59160.1"
                     /translation="MPKEFKLHSPYEPKGDQPKAIEELAEGVAKGYRHQTLLGATGTG
                     KTYTMAGVVEEVQKPTLVIAHNKTLAAQLVSEFKEFFPENRVEYFVSYYDYYQPEAYV
                     PQTDTYIEKDASINEDIDKLRLAATSALFERRDVLIVASVSCIYGLGSPADYLELSSH
                     LKVGKEKSRKEIMRELSMMQYSRNDIDLQRGHFRVRGDVLEIYPAYRDQAIRVELFGD
                     EIERITRINTVTGEIMEDMDEITIYPASHFVTPEDKIERAIKTIKEELVERLEELREQ
                     DKLVEAQRLEQRTNYDLEMLEQMGFCSGIENYSRHLSGRPPGSRPQTLLDYFPDDFLV
                     FIDESHMTIPQIGGMFAGDRSRKEKLVEYGFRLPSALDNRPLNFAEFEEVVPQAIYVS
                     ATPGPYEHEHSQQIVEQIIRPTGLIDPKVEVKPVEGQIDDLLEEIRVVVEERNEKVLV
                     TTLTKRMAEDLTEYLGEAGIRVRYLHSDIDTLERSEIIRDLRLGEFDVLVGINLLREG
                     LDIPEVSLVAILDADKEGFLRAERPLIQTIGRAARNVNGKVVMYGDKITDSMRKAIDE
                     TERRREIQKEFNEKHGIEPQTIRKPVREVLRPEKLVIEDSKEKVYSTDDEDNFAELSK
                     IELQNKIIELEEEMDEAASNLEFEVAAQIRDEIEEIKEELSNR"
     gene            complement(36048..36914)
                     /gene="sdaAA"
                     /locus_tag="VKN64_02630"
     CDS             complement(36048..36914)
                     /gene="sdaAA"
                     /locus_tag="VKN64_02630"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HMA59161.1"
                     /translation="MQYNKIKELIDKAEQENKKISQIVIEEEIYEREIERETVIDEMK
                     KHLKIMRESISEGLTPGHKSATGLTGGEALTLQNSNKSLNTPLYKKVIIKALAVSEVN
                     ACMGKIVAGPTAGASGIVPAVLITLTEEHEISEDKLIDALFTAGGLGEVVSKKATLAG
                     AAGGCQAECGVGSAMAAGAAVELMGGTPQQVGDAFALALKNLLGLVCDPVAGLVEVPC
                     VKRNGFAASHALTAVNMALSGISSVIPPDEVIAAMMEIGDLMPVSLKETSEAGLAKTE
                     TGQKIAEELQDF"
     gene            complement(36928..37584)
                     /gene="sdaAB"
                     /locus_tag="VKN64_02635"
     CDS             complement(36928..37584)
                     /gene="sdaAB"
                     /locus_tag="VKN64_02635"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HMA59162.1"
                     /translation="MSVFNVLGPIMIGPSSSHTAGAVKVGYLARSIFKGKLVKADIYL
                     HGSFARTFKGHGTDRALVGGLLGFKPDDERIRDSLNLAKERGVEIELHLSDLRGVHPN
                     TAKLELFGENKEMTIIGSSIGGSRIKIIQIDDYEVDFSGEMPTLWVLHKDEPGEIGII
                     TSILGSYKLNIASMEVFRNKKGEVGSAIIELDHKVSDYVISHLQNMDNIYQVRYIEPF
                     "
     gene            complement(37602..38756)
                     /locus_tag="VKN64_02640"
     CDS             complement(37602..38756)
                     /locus_tag="VKN64_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMA59163.1"
                     /translation="MFRNKKKLLAVLLVITILTVGGFKVIADDLTPLKNLQNVIYLID
                     NYYVEDKNLEDLIDSAITGMLKELDPYSSYLSPDEYEEMQIEFDGEFGGIGIVITVRE
                     EKLTIVSPIKNTPGDRAGLKSGDVIKAINGKLTSKMSQQKAVDLMRGEPGTHVTLTIS
                     REDKEEDFDVDITRANVEIPYVEHNMESDTIGYLSIYQFGSEVGYKTEEALTDLKKQG
                     AEALILDLRTNPGGILDEAVKVASNFIEDGTVVTVKSRRKEDEVYNTVSDIEAVDMPM
                     VVLINEGSASASEIVAGAISDYGRGKLVGQKTFGKGTVQTVIPLGDGSALRLTTARYY
                     TPNGTFIHEKGIEPDIKVEFDQESEEDAQLQKAIDLLKDKVRTKSLDKAS"
     gene            complement(38789..39523)
                     /locus_tag="VKN64_02645"
     CDS             complement(38789..39523)
                     /locus_tag="VKN64_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HMA59164.1"
                     /translation="MKDKTEILTLKINNITMQQMVSELINTIYNKEAKKLIYTPNSEI
                     AVRAYDDTSFSKLLNRADFLVPDGAGLLLAARLLGEPLQERVAGYDLMRKLLNEIEDK
                     DISVYFLGGKPGIIDSAVQNIREELPELSMAGFHHGYLDSDSKEMIIEEINNKKPDIL
                     LVGMGVPLQEKFFDSHFEDLDVQIGLTVGGSFDVLSGQSKRAPLWMQKMYLEWFYRLL
                     HEPKRIKRMMALPRFLFLVVKEKYDI"
     gene            complement(39525..40589)
                     /gene="csaB"
                     /locus_tag="VKN64_02650"
     CDS             complement(39525..40589)
                     /gene="csaB"
                     /locus_tag="VKN64_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HMA59165.1"
                     /translation="MKILLSGYFGFNNLGDEAILASMVDMIRAETDEAELTVLTQNTA
                     ETKVKYNTETIYRYDIFKIAAAMKKADIFISGGGSLLQDVTSLRSVPYYLGLIFLAQV
                     FNLKTVFFAQGVGPVNNSFYRFLIKKVLSRVDFLSVRDLNSKLFLEEIGIERNKIKLI
                     DDPVYGLNICKGSDSEVSNTKKIGVSVRSWQDNSYLQKVAEFLNYLIKKEEYTVTIFP
                     FHEGEDERVSEQLKNMLYTQAEIIDYTDDMKKINNLYSTFDVFIGVRLHSLIFAAINC
                     VPFIGISYDPKVESLIQEMNYQPNITTDNITEEQLIKSYDIIKDEKKEIKDRIDRIVK
                     VKNERVKGSISRLLTDLKGE"
BASE COUNT        10910 a         9140 c         6809 g        14096 t
ORIGIN      
        1 tttatgacga ggcttttcat ctcaaaccgg ttggcatagg ctttatcaag ttgggtctgt
       61 agtttttctt ctaaagctgg tatgtcaggc tgtttgagtg taaaatctgt attatcaagt
      121 tctaataagt cagccagcct ttttttggca atctttagat cattttcagc tgttagtatg
      181 gactgctttg tcttattgag ttcaatttta acttcattaa catcactttt tacaacaaga
      241 ccggcttcat aatttttctg tacactatct aaatgacttt caactaattt tagggagttc
      301 ttttgaattt ctaccaggct ttctttgatc agtacgttaa aatatgattg aataacggca
      361 tttaaattat catttaaggt ctgttcgtat ttaagattat ttaattggac cgctttttca
      421 ctcaggttaa cgctatttct aacctggccg cctaaataga gcggtttgtt tacggttagg
      481 gttgtattat aattatcctg gggttggtct tcaaaagaaa cgggaataaa ctggtattta
      541 gtcgctggtt gaccaaaaaa ggtggtctgt ccagtaggtc tcaatatata ctccgatgga
      601 gattggggtg cttctcccaa tcttgtatag gaggtctgaa gagttacctc tggggctaaa
      661 cctttttctg ctatttttaa gtcaagttgg gaggcctgta tatctctttt ggcattttcc
      721 aactgattac tgttatttaa aaagtactga actgctttat ccaggctcat ctcctcagca
      781 gatattgtaa ctgtattagc tattaaaaat agtaaaatga aaaaagttag aaacaattta
      841 ctttttttca ttatcaggtt ccctcctatc taaaccatgc ataataaaat catagatttt
      901 tttagttctt gtttcaatat ctcccttaat accacagcta aaattattat gggcactatt
      961 ggtggtatta attatcataa gggcatattc atcactttta cccgcccgaa aagttccttt
     1021 ttcaatacct ttttgaattg tattctctaa gacctgcacc atctgtttgt gtcttttttc
     1081 catcgtgtct ttaaaatctc tgtttatcac atctgtatta ctaatcaaca tccttgccac
     1141 atcatagttg ttttcaataa attcgatcct gtctcttata tacttgtata taatatcggc
     1201 tggtttcata tcgctttttc ctaattttaa aacttgatta aaatatgact ttcctttttc
     1261 ttctataatc gataaaaaga ggtcttcctt actctcaaag tgccagtaga tcgttccttt
     1321 cccgacatca gcttgatttg ctattttgga catagaagta tcactaaagc ctttatcagc
     1381 aaataatttg atggcggttt ccataattgt agttcttgta tcttctttca tctaatcact
     1441 cctatttttt ctcgaccgac cgttcagttt agtaaattaa tcttaacaaa tattatatgg
     1501 gttgtcaatg tttatttttg actatagaat ccttttatga ttgtaaatat ttacaattaa
     1561 tatttcagga ctacgtactt gaaatgattt aattaaaata gaaaaaccat caaccctgtt
     1621 ataatggatt aatggtttaa tagatatata ttttatatta ttatttaagg ggattatatc
     1681 ccatatttcg atgcagatcc gcaatcagct tatgaccctt agctgaaaag tggataccgt
     1741 ctttttgaat atacacttcg ggcagcatgc tgttacccgc ctggccattg aaggccttat
     1801 aggttggtac cactttgata ttattatttt cagctgcttc aatcagatac tccctccagt
     1861 cttcataagc atattgtttt aattcctgat aaaaattatt ttctttccag gtctctgcta
     1921 ttgcaggcgg aatacctgtt tcggctacca tgattaatgt ttctttggga tcggccagcc
     1981 tggtaatctc agcgaagaga atattaaaac tgtcccccat ttttttggtc gtcatattta
     2041 attctttttt atatacccgc tctgttttat agatataggg catgatctca tgccaactta
     2101 tccagattgt gatgatatcc gcatttttta tatccattct aaagacttca tcggtggtta
     2161 tggcctgaac ccaacctaag aggttcctgc tttcaagttt tgcccagtta attatctcaa
     2221 cttcaacgtt tagatcttgt tctatatatt ctgcatatac ccttaaataa ttttcttgtg
     2281 gtttaattcc atacccggcc ggagttgaat tgccgatcgc tactaatttt aaaggattct
     2341 ggggtacttg agaataggcg ttttcattta aaatactaac tatcagcaag gataagatta
     2401 tcaggagagt caaacttaat acttttatat ttttcaacat gtccccctcc tccctgagat
     2461 aaattatttc ttttataatt agaaaacaaa aaatccctga ggtatgtatt ttgtctcagg
     2521 gatttaacgg gctgattaat tatcttttgc aaactaatta taccattaaa ttaatgtata
     2581 cacaataaat cttgtattaa ttggcaattt aaaaagcccc gcagttttaa cgaggcttta
     2641 ggaggttttt tataatatgt gtttattgat tttgggttat gttgttataa ccaccgcgcc
     2701 taccggctga atgtctaccg cgaccttgac ctcgctggga actgtcaact tgatttcgac
     2761 cgtatttatt ataagtattg gagctggcta ttttattacc atttccggct gcaagtccca
     2821 tcctatattc gttttcagct attttttgaa gctgctcttc tgttaaaact tcttttattt
     2881 cctttaaata atctgttctg gcctgatcaa tctgggctct cagtttttct acttcagttt
     2941 ctagggcagt gattttttct tgcgattcat tttttaaaac ttcttctctt aattctcttt
     3001 gggtagactg tagtttttca acaaattgat ccttttgcgc aaaatgtttc tcttttaatt
     3061 ctgcaacttt ttcggcctgt tcttcatcca gatctaatac ctctacctcg gattgggcgt
     3121 tctgttgaaa agcggggcgt tcaatattat tatcgattct attacctgct ccgcccatct
     3181 tatttcccat ctgggcctca actgcaaaag ctccaaatgc taaaactatt aatgataaaa
     3241 ctaatgttga tactattact ttcttcattt caatctctcc ttctttttat tttttctatt
     3301 tttttctact gtaaatacat tataaaaatt aattatgaag aaagtatgaa aggaaagaat
     3361 taatttttaa atttttaaat atttctttaa gtaattaaaa aattaaagag gtggggaagc
     3421 cccacctctt aactatctat atatttaatt ttgcatttct tacttgttaa tgttatagaa
     3481 agcattcata ccggcatatt tagcagaact gcctaaggcc tcttcaattc tgagcagctg
     3541 attgtattta gctactcgtt ctgagcgggc cggggcaccg gtcttaatct ggccggtatt
     3601 cattgccacg actagatcag aaatggttgt atcagaggtc tcaccactgc ggtgtgaaac
     3661 tacggcagta tagccggcct ttttagccat ctcgattgtt tcgagggttt cggttaaagt
     3721 accgatctgg ttaaccttaa ttagaattga attacttaca tcgttttcaa ttcctttttt
     3781 aagcctttta ctattggtta cataaaggtc atctcccaca atctgtactc tatcaccaat
     3841 tctttcattt aataatgccc agttatccca gtcgtcttca tccagtccat cttcaattga
     3901 gataatggga tatttatcaa ttaagccttc atagaaatct accatttctt ccggagtccg
     3961 cataacgcct tcgcccttga agttatactt gccgtctgca aagatttctg tagcggctac
     4021 atcgagggcc agcatgatat cttctccagc ttcatagccg gctttttcga ttgcttcaat
     4081 tattacatcc agggcctctt cattggaaga taggttaggt gcaaatccac cttcatctcc
     4141 cactcctgtc cctaaatctc tggactggag aaccttcttg aggttatggt aaacttctgc
     4201 tcccatctgg agggcttctt taaagctctc ggccccagta ggcataatca tgaattcttg
     4261 aatgtctaca ttattatcag catgttctcc tccatttaag atattcatca tcgggacggg
     4321 caggagattg gcacttactc ccccgatata attaaagagg aagtcatcgg aagctgaagc
     4381 agctgctcta gctactgcta aggatacacc cagaatagca ttagcgccta atctaccttt
     4441 attatctgta tcgtccattt caatcattag attatctatg aggatctgat cacgtacatc
     4501 cctaccaatt agctctggac cgataattgt atttacattt tttacggcat ctaatactcc
     4561 tttacccata tagtcatcgc cgccgtctct taattcaacg gcttcatggg caccggtaga
     4621 agcacccgaa ggtactttgg ctctaccgag ggtaccatct tctaaaacta cgtctacttc
     4681 aatagtagga ttaccgcgcg aatcaaggat ttgacgtgca taaacatctt caataaacgg
     4741 acttatcata aaaaatcacc tcttttttta tttttcaatc agagaattac ctgtcatttc
     4801 atctggttta tcaatatcta agatatcaag catggtcggt gcgatatccg ctaatctacc
     4861 atctttgaga ttaacttttt cttcagaacc gacatacatc atgataaccg ggttagatgt
     4921 gtgagcggta tgaggttctc ctttttcatc tatcatctgt tcactgttcc catggtcggc
     4981 cgtgatcaac atctgaccac ccatctcttc tatcttgggc actatttcgc tcatacattg
     5041 atcgatggcc tcgagggctt caacggctgc atcatagaat ccggtatgcc cgaccatatc
     5101 gggattagcg aagtttaaga tgattacatc atagatgtct tcatctaatt tttctaacaa
     5161 tctatcttta accttaaagg cactcatttc aggttgtaag tcgtaagttg ccaccttagg
     5221 agaaggaata agttcgcgat cttcaccctc aaactctctt tcttctcccc cattaaagaa
     5281 gaaggtaaca tgggcatatt tttcggtttc ggcaatcctg agttgtctca tattatggtc
     5341 acttaatact ttccctaaga tattatcgat gtgaagttta gggaaggcaa tcggtaggtc
     5401 aaagtcttca tcatactcag tcatgcagac gaaatatagg ccgttggggt gtttattcgg
     5461 tctttcaaat ttatcaaatc catcaagccc aagcgccctt gtaatctgac gggccctgtc
     5521 agccctgaag ttaaagaaga tcaaggcatc ttcatctttt acagttgtaa cgggttcccc
     5581 gtcctcaata acaacactgg ggatgacaaa ttcgtcgtta tcaccgcgtt cataggactt
     5641 tttgaccgct tcttctgctg aggaaacttc gattccttcc ccttttacat aagcatcata
     5701 ggccttttta gtcctatccc atctgttgtc tctgtccatc gcataatacc taccactgat
     5761 agtagctatc ttaccgagac ctatttcctc cattttttct tctaactgtt caatataggt
     5821 taaggcactc tgcggtgggg tgtctcttcc atccaagata gcatgaacat aaacattgtc
     5881 taaaccagct gttttagcca tttctagtaa gccgaataag tggtttatat gactgtgtac
     5941 acccccatct gataaaagtc ccatcaggtg aagggcacta ttattatcat ttactttctg
     6001 tattgctttc tgtataacat cattttctaa tatcgaacca tcttccacag ctttggtaat
     6061 tcgggtatat tcctgaggaa cgatacgacc tgcccccagg ttcaagtgtc caacctctga
     6121 attacccatc tggccgggtg gtagtccaac atgttcaccc gaagcttgaa taatggaagt
     6181 ggggttttcc tctctcaact tatccaagaa aggagtgtct gcatttttta ccgcattttt
     6241 ttctatctgt tctgactcac cataaccatc caggatgatt agggcaagtg gccgtggtct
     6301 gttcattttg acacctcctg ttctgtcttc tacttagttc tttacattac atttcggaag
     6361 tctttctaac aatagctgca aatgattcag cttctaagct ggcacctcca actagggcgc
     6421 cgtcaatatc actttctgcc ataaattctt caatattata tggtttaaca ctacctccgt
     6481 actgaattct tacatcatca gctgcatcca gatattcttc ctctattaca tctctaatat
     6541 agcttattac ctgattggcc tgtttggcag ttgctgattc gccagttcca attgcccaga
     6601 ttggttcata ggcaattaca gtctggctga cctgtttttg gttaagccct tttagggccg
     6661 cttttacctg tttttccacc ttatcacgtg tttcatttgc ttttctttct tctaaagatt
     6721 ctcccacaca gattattggt ttaattccat gctcaaaggc ggcatgaacc tttttgttta
     6781 cctgttcatc agtttcatta tagtactctc ttctttcaga atgtccaatt atgatatatt
     6841 ctaaacccag ttctttaatc ataaccgggg aaatatcacc tgtaaaggca ccgctttctt
     6901 cccagtgcat gttctgggct cccactttta cattactgcc agccgctact tttttgacag
     6961 cctgcaggtc aatagcgggc gggcatacac ctacttcaac attttcaata tctgcaactt
     7021 tttctttgag agaactaact aaatcttttg cttctgaaat agttttgttc atcttccagt
     7081 taccagcaat aaaaggtttt cgcatctttt atccctcccg tttatttaag atcatcaatt
     7141 gcttcaacag caggtagtgg ttttccttcg aaaaatctta aggaagctcc tccaccggtt
     7201 gaaacatggg tcatctcatc aataagatcg gcttgtttga cagcagccgc tgattcacca
     7261 ccgccaataa cagtttttgc atctgtttca gccagggctt tggcaattgc ggtcgttccc
     7321 ttggcaaact tttcgaactc aaatactcca atcggaccgt tccagattac ggtttttgcc
     7381 tttttaatga ttttagtgta ctcttctatt gttttgctgc cgccgctatc caaaatctgc
     7441 cagtcttctg gtatatcacc gacatcaact tctttggagt tggcatcttt agcaaactta
     7501 tctgcaatta ctacattttt gggtgtgagc agtattacat ttttttcttt tgccttcttg
     7561 atcaattcac gagctgtttc aagtttgtca tcttcgacca gggattctcc tacttcatac
     7621 ccctgggact ttaagaatgt attggcaatt ccgccgccgg ttaaaatata gtctacttta
     7681 tctaagaggt tttcaattac ggaaatctta ccggaaattt tagctccacc catgattgca
     7741 acaaagggtc tggctggatt atccattaca tctcctagcg cattaagctc tctcatcagc
     7801 agaaaaccgg ctgcagaagg taaatattcg gtaactccca ctgtagaagc gtgggctcta
     7861 tgagctgccc caaaagcatc cagaacaaag atatcagcat tttcagctag ctttttagca
     7921 aattcgggat cattggcctt ttcaccggga tagaaccttg tattttctaa aagtagtaca
     7981 tccccgtttt ccatattgct gaccgctttt ttcacttcat ctccgataca gtcgtcaacc
     8041 ttaacaacct ctctatttaa gaggttggca agctcttttc cgaccgggtc catcttgaat
     8101 tcttctttga cttcaccctt tggtcggccc aaatgggaca tcaagattac cttagcatct
     8161 tcattcatta agtatttgat ggtcggtaac gcagcttcaa ttctattggt atctgttacc
     8221 ttgccatctt ccaatggtac gttaaagtcc actcttacta aaactctttt acctttaaaa
     8281 tcataatctt ttagtgactt tttcaaagta gaaaacacct ccgtttttta aggtataaaa
     8341 aggggttaaa gcataatgct tttaacccct ctaattttat attcaaatta tatgcctaat
     8401 tctttcatct taattgcaaa gtcgtttaat ctttgtgcat aaccccattc attgtcatac
     8461 caggaaagta atttaaccat attgccctgg attactttgg tatgattttt gtcatagatg
     8521 gaagaatgag gattgccaat aatatctcta gatacgatag gatcttcatt gtattcaaga
     8581 attcctttca tatctccttc tgcatattct ttcattaccc tattgatatc ttctactgta
     8641 acatcttctt ttaatgttac aactaggtct accaatgaac cggtaggtgt ctgagttctt
     8701 acagccatac cgtccagtct accttttaat tcaggcagta ctttggtggt agcgacagca
     8761 gcaccggttg ttgttggtac gatattttcg gcagctgccc gtccgcgggt aattttcttc
     8821 cacttatatg gtccgtctaa tatattttga gaagatgtat aggagtgaat ggtggtcata
     8881 agaccttttt caattccaaa ttcatcattt aaaatcttag ctattggtga taaacagtta
     8941 gttgtacagg aagcatttga tattatatta tgattatctg ggtcgtattc atcatcattt
     9001 acacccatta caaaggactt atctacattt gtagcaggtg cagaaattat tacttttttg
     9061 gcaccggctt cgagatgctt tttagcatct tcgccatcgg tgaacagacc agttgcttca
     9121 taaacaacct ctacgcccag ttcaccccag ggaagttctg ccggatcctt cttgttatat
     9181 accttaatct ctttaccgtc tacaattatt gaattatcag tatggtcgat ctctccatca
     9241 taagtaccat ggttagagtc atacttgaga agatatgcta atatttttgg ttcaaccaga
     9301 tcgttaattg ctacaaagtc gatatcagga tttttaacgc cacctctgaa agcctttcta
     9361 ccaatagctc caaaaccatt aattgcaact tttacactca tctctttatt cctccttatt
     9421 tatattataa ttagaattga ttaacattga tgttaggtat taaacattta cttatttaat
     9481 ttttgctctt tttcacctcc cttaagggct ttaatctctc ttgcggttag ttcatccgtg
     9541 attaatatat cgtgatattc aggtgaaact actgaaataa tagcgcgggc tttatcaaca
     9601 ccgccggcta cggcaattac tttatctata ttctttaagt ctgccatgct taaaccaaca
     9661 ctagcagttg aatatacaac ttcaccttct tcattaaaat agaggccaaa ggcttctgca
     9721 attgccccct tgtcttttaa tttttcgatt tcggcctctt ccatatcccg tcgggctgac
     9781 atcatttctg cggttccaat gccgtggact aagatattgg ctttctgtag ttcatctaat
     9841 gtctttttaa ctgagggttc gttggtgatc atcgccaggc tttccctggt aatatcgtcc
     9901 gggatatgta agagtttgta ttgtcctccc agttttttgg ctatcttgga agcgatcgta
     9961 ttggcctgta tttctacatt ctcgcccagt ccaccccggc ctggaattac ggttacatct
    10021 ttgggctctt ctaaaaatac cattgtttca gcaacctgag ccagggtaga acccccggta
    10081 actgcaatga tatcttgatc atttatcatc tcttttaaaa agcgggcggt aaaacggccg
    10141 atctctttat tgataatttc ggaatctttt tcaaccggta cgatcattac cttatctata
    10201 ccaagcagtt tttcgatttc gttttctata ttggcaatat ctttgatctc tttcatgtag
    10261 gtatcaagtt catgtaaaaa atcttctcca ttctctgtaa ggatagttcc gctcttagtg
    10321 atcttgatat atcttttttc atacagaaaa tcgagatgct ttcttacatt cctgatactt
    10381 aggtccaatt tccgggctaa attgcggcgt ccgatcggct ggtgattgaa aacttctttt
    10441 agtatattat atcttatttc agcaatactt atcaattcag ggacaagctt tttctgtatt
    10501 tttaacaaat aattcatcta atccctccta ccttcgggat tattttgtcc gtgcggcaca
    10561 attttgtccc gggttatcta taaatatagt attcaatttc caatatactt tattaaatat
    10621 taacgcgaaa attcctgcct taaattgaat atttttttct gcgttttacc gaggaaggga
    10681 tccctagttc atttctatac ttagctatcg tcctgcggga aagctttaag ttctgttttg
    10741 tacgcagtaa ttcagcaatt gcattatcac ttagcggtga acttttatct tcttccttga
    10801 tgatttcaat gataattgcc ttgatactta ccgtggaaat atcatttact cccccgctga
    10861 aaaagaactt gaagtcaaat ataccgcggg gagtctggat aaacttatta tctgtcgctc
    10921 tactgacggt tgattcatgc atatcgatct catcagcgat ctcctgcatg gtaagcggtt
    10981 ttagatactt gacaccatca tctaagaaat tcttctgttt atcagctatg gtcttagcaa
    11041 tacggtagac cgtcattctg cgctgttcga tacttttaat gatccagagg gctgatttga
    11101 acttatcttc cagatattca taggtatcat cttttttaca cttctgcatc atttcataat
    11161 agtagggatt tattctaagc aggggtgaag ctttatcatt aaggactatt acatattcat
    11221 cattgacctt tttaatgacg atatccggca ttatatactg ggtctttttg tgatcagcca
    11281 ctaaagaggc cggttttgag gttagtttat tgataatatg caaggccttg cttagcctat
    11341 cttcactcca gccggtttta tctagtatct ccttatattc ttctgttact attaaatcca
    11401 ggtggttacc gattaaaagt tcagcatcat ttgttttaac atccatctgt tttagctggg
    11461 ttaagtaaca ctcttttaga ttagtagcgg caatgccggc tggttctaat ttttggatct
    11521 ttttaataat gcttttaaca taatcaatat cctcatcaag ttcctcggct atttcatcta
    11581 aggaagttga taaaaggcct ctttcatcca gattacctaa aatatattcg ccggcttctg
    11641 tttcatcatt atctaatact tcatagagct ggttctctaa gtattctaaa agattcggct
    11701 ggtaagtaac catattttca tagttgaaat cttcctggtt gtaattactg ccgtacctat
    11761 aactctggta ctggtttttt attctatcat caagatggtt ttcgttttca tcatgttttt
    11821 ctaaaagggg attatctttc atctcttctt cgacatattc ctttagttcc atactggaat
    11881 actgaagaag tttaatagcc atctgaagtt ttggagtcat tactagttcc tgtttttgtt
    11941 ctagatctac gcttaaatca agagctagat ccattttttt cactccttat cttttcggcg
    12001 atctttttta accgccgtat cctgttatat acacctgatt tagtaagttt aggttctaac
    12061 atctctccta attcttttaa actggcatag ggattgtctt ttcttagttc ggcaatttct
    12121 cttaaactct cagttatatt atctaatcct ttaatttctt caataagttc gatatcctcg
    12181 atctgctcca tagcggcctt gaccgtttta tccagattgg ctgtttcaaa gttgaccctt
    12241 ctgttcacat tgttcttgat ctctttaaaa acctgggtgt tttctaactt taaaagggag
    12301 ttatatgccc ccatcaagtt caacatggtt gctatgtcat catacctttt aagataaatg
    12361 acctgtttct tcttgtgttc agtcaggtga gggtctaatt caaagtcctg catcaagtca
    12421 ataatttcct cggcatgtga cttatgttca cacctgatct ctaggtggta ttcagcccct
    12481 ggtttattga ccgatccacc tgccaaaaat actcccctta gataggccct cttgtaatct
    12541 cttttatcct tgatccagtc tgccactgta aagaggagac tatgggactt atttaagata
    12601 cccaggtttt ctaataggtc ataaacacct ttttggggtg ttatgaaaag ttcataaatt
    12661 ttgcgcctgc ttaaattgtt ctttttaacc ctaatttcac tttcaaactg atactcttct
    12721 ttaatatatc tatagatcct tcgggctaca tcaccgtgag aaacacttat ttttacggcc
    12781 attttgccgg aagaaagtaa aattgaacca cccattctta tcagggctgc tagttcagcc
    12841 tgatgtatcc ctttttgctc aatttcaaga tgggctaatt catgttttac atcatcagaa
    12901 aaagacattt attaaaatca ccaactttta ttatagttcc actaaaattt cggctagctt
    12961 gtggggatca tgtctgatat agtcaccggc ttgaattagc ttaccttcta ctaccttaat
    13021 atctaattta ttaagctgct caatatcatt ttcaaccgga taggctccct cttccgcata
    13081 cttatctaaa aggtctttcc tgctgccgtt tttatttatt attgtatagt caaagatctc
    13141 agccccaata tggttataaa cggtcttcaa gtggtcatag gctgaatagc cggtcgtctc
    13201 accgggctgt gtcattacat ttgtaataaa gatctttttg gctttacttt ccctaatggt
    13261 atcgaccatt ccctttacca ataaatttgg gagtagagaa gtatatagac tgccgggccc
    13321 cacgataata tagtccgcgt ctaaaagagc actcttggcc cgctcggtaa tttctacaga
    13381 actgggttcg agataaaccc cctgaataac tttgttttca tcgggaatct ctgattcacc
    13441 caaaatttca ctaccgtctt cataaactgc tcccaggttt acattttgat tggtagaggg
    13501 gagaacctta ccctggatag ctaaaacctt tgaagattct tcgactgctt cttcaaagtc
    13561 acctaatact tctgtcattg cagcaataaa gaggttacca aagctgtgtc ccctcaagtc
    13621 acctttttta tgaaagcggt actgaaataa atcctccatt aaaggctcct ggtctgctag
    13681 ggcaaccaga cagtttctga tatccccggg cggtaaaata tccagttcat ctctcagctt
    13741 accggaacta ccgccgttgt cagctactgt tacaattgcc gttatattac tggtaaattc
    13801 ttttaaacct cttaagatat tggaaagacc ggtgcccccg ccgatcgcaa ccacttttgg
    13861 tcctttttgc agttgatttc tcttatataa aacatttacg aaatcatcat ctttgtatat
    13921 ttccttattt aacttatcca caaagcggtt accggcataa gttagcattg ccagtgaaat
    13981 aattatcaag gcgataccta ttatcaggtt cgtataatac ggaagtgagc cgcttaaatg
    14041 ggtaatagct tcaaagatca ttttaataat ataatcaaca atatttatgt gtaaaagtaa
    14101 tgcaagacca gtactgagca aaagtaagcc aataataccg agaataaacc atctctttac
    14161 acccagccca ggataaagcc actttagaaa tttcatctta ccactcctgg ttacttatta
    14221 atatctcggt ggttaaccgt aactttatat cctttactat ctaaaaagtc ctttaactca
    14281 attgcggttg tgactgaacg gtggtgtccc cccgtacagc cgattgcaat acttaaatga
    14341 ctcttcccct ctttttcata ttcgggaatt agaaattcta tcatgtcaaa aaatttatca
    14401 taaaatcttt tcgtgatcgg ccattttaag atatagtcct taacttcttt atcttccccg
    14461 gttaagtcct ttaaagaatc tacataatag ggattggcca taaatctaac atcaaatacg
    14521 ttatctgcat ccagtggtat accgtactta aaaccaaaag aaattatatt gatagtgagg
    14581 gactgcatat cttcatccac atccagataa tctcttctca atttctgttt taattcctta
    14641 attgagatac tggaagtatc gattacattg tgtgcttttc cccttaattg ttctaatctc
    14701 cttctttcct ccctaatcgc atccaggacc cgaccttctt cctctaaagg atgtcggcgt
    14761 ctggtctctt tatatctatc gactaaagcc ttatcagagg cttctaaaaa aagtatctcg
    14821 tggttaatct gatgctcttc taagtaattc agcttttctg tcagggcatc aaagaatttt
    14881 cctcctctga tatcacagac cactgctatc ttatttaatt cagaatgtaa acaaagttca
    14941 gcaaacttgg aaatcaaggc tggtggtaag ttatccacac agaaataacc gctgtcttca
    15001 aagaagttta aggttgtaga tttgcctgct ccagacattc ctgtaattaa gataaatttc
    15061 attaatttaa cactcctagt ttctcttcca ctctaatttt ataacattat caatagcttg
    15121 taaaataggt ctaaactagg accggaaaag ccttttttaa aatcttgaac tcggctggca
    15181 gcaaaccggc actttctcca tcaatatcga ttaaaacccg atccttggaa tctattttaa
    15241 tctctttacc ctttagataa tcaatcttat ggtaatcgat atgggttccc cggtaagctt
    15301 tatataggtt taacagggtt tccagttttg tgaggttacc caaaattact atatcaaata
    15361 agccgtcatc gattacagcc tgaggtgcta tcggcattcc accggcaaag tatttaccca
    15421 gacttaccat aacactgttg attaggcctt cttttattac ctcgccatcg attgtaagtt
    15481 tcatatcttt attgtcatat ttcactacag tccggatggc ccctataaga taagtcaaaa
    15541 gaccgcccat cagttttgag gaacgattca catattccac cgtctctccc ccgataccaa
    15601 catcagcctc attaacaaag tacctttcaa cttccttttc atcgaagttt aagtacttca
    15661 tcttgccgat atctacccat tttctttcat actttttata ggtattaata acggtttcaa
    15721 tacttttatt taaaccgagg gactttatat aatcggaacc ggtcccctgg gaaaagacaa
    15781 gtaagacagc ctcttgatta atcaactgac cgtcctgata aaatccatta actatttcat
    15841 taacggttcc atctcccccg actgccataa tagttttata gccatcattt aaagcggttt
    15901 tagttaagtt taccgcatga ccgggataaa gggtctcatt aatatctaaa tttaatttgg
    15961 cctttttaaa tttctgttca taggcaggcc atctcttacc cgttttcccg ttagaagaaa
    16021 ccgggttgat gatcgtaaat attttattct gctgtggatt gaattcttta attgaatttg
    16081 cttgcaacat ttatcaccta tattattaat ttcgttttac acagcaattt tcctctattc
    16141 ttttctaatt aaacaaaaag aaagaccacc atctcaggtg gttatccttg gtttaaaact
    16201 gattgtgctt tttttagaaa ggtatcttcg gatactgcta aaatctttaa gacttctgtc
    16261 ttttttatct ttaacttttt gttcaaatag aatagatcgg gattgccttc cacaagctcc
    16321 agggtagcat aacccatcgc gttgttgacc gaataaaact tcttatagcc gctccattcc
    16381 tcaggtatgg cctggtagta atcgatcaaa gccctagtat ggcttttaac atctgcgacc
    16441 attttattaa gggcttttaa agcctgttct tcattattct gatcgattat ttcactctcg
    16501 gaaatcccga gttctttaag cggccagtaa tgttttttgt ggtttatgat atccagcttt
    16561 atgtccctta ttatatttac cttttgtaaa aagaggcccg attctacgaa atttttttcc
    16621 agtacctttt tatctttacc ctcaatatta ccaagttcaa taattaaatc agttaaaaaa
    16681 cctcccactg tcccggctac ataatagcag tattcgttaa gttcactaaa ggtttcaacc
    16741 ggtttatcaa ggtaagactg cataccctga ttcatttcac ttaaccagcg gaaagatatt
    16801 ttttgtacct cttcattgag ggtattaaaa caacttatca atttatcggt gttttgaact
    16861 agttttttta taggaccctc gaaataatcg gcctgtcctt taatattggc aactaattcc
    16921 tgctgtctag actccgagat attaggatcg aacaatttga caacttcgtt catgtaagct
    16981 tttctctggg aactattaat ctccccttca tcttcaaagc tgtctaaaag tctatcctga
    17041 agatatgtaa tcatgacggg ctctcttatt cttttatcta agaggggtat cgtcaaagca
    17101 aaacttctcg ataccttctg cattatacta cggcagaaat caagtgcctt tttgttcttg
    17161 tttttcagca tttccgttaa caccaagcct tttttgaatt tttttaatag aaataaatat
    17221 gagaacggat aatatcaaaa agataattgt gtatccataa taatcttgtt tttctctttc
    17281 cttatcatgg ccaatataga ccggttctat ctgagcataa ccgatctcgt aagcatccag
    17341 cattttttcc tggctgtact caggtggaac aattaaatca atcacccggg gagcatagtt
    17401 aatatcatga tcaccttgga cagcaaaaaa ccaggagttc aactcatctc ttattccccg
    17461 gaagtggtca tagccgaagg gatcaaaccc ccctactaaa atgaaaagat tggctccctg
    17521 cagagggccg atcttctcct ttgcaatctt taattttatc cagttatcct gcactttaag
    17581 ctcgggatta ttaattaccc agggcacatc ttccccatcg actgtaaaat caaccacctg
    17641 tctattccga tctaggggtt cataaaggct cacccaccag ccgtttatct taagcagttt
    17701 atcccagggg tattcgggat taagtttcac cctggcccct tcttcaaaga gggcaattga
    17761 tcctcctttt tcattatcta tgtaaatctg gaccatgggg tgactgaaac caaaacggga
    17821 gttccagaca tcgaccagtt ttgtaaattc aaattgaaac tcatattgag aactgctttc
    17881 atatatttta aactccacaa tatcaaataa tccttcctct ttttgaaaaa cctcatgctg
    17941 gggataaagg tagtaaccgg ggccgtgatc atcaccagcc gggtcaactt taaaaaatat
    18001 taaattgtct tcagcctgag cagccgcata gaaagttgta atgaaaatta ttagcgaaat
    18061 ggtcaggaca tacttatgac ccgagctcat tttattcacc ccgtcggact atattattgg
    18121 ttttgataaa ggtaatcaca catcgctacc gctattgcac cctttactcc ggtatcttta
    18181 cccagtttgg cttcttttaa ctgcagatct tttagatttg gctttaaagc cctttccttt
    18241 aaactggctt ttaattcttg cataaatagg tcctgggcat gcataactcc tccacccagt
    18301 aaaataatct ccgggtcgaa aagattaacc acattggcaa tacctacacc cagaagctga
    18361 ccggttaatt taaagatacg aatcgcttcc tggtcacctt ctcgggcagc tgccgcaatc
    18421 aaaggtgcgt ctactacatc aggattacct tcactatatt cataaattag actgtcctgg
    18481 tgaacagtta atgattctct gccccttctt gcaatagcag taccagaggc aactgcttca
    18541 aaacagccgt aatttccaca accacattga tagtcactgt cggggattaa ggtcatatga
    18601 ccaacttcgc ccgcatttcc ttttccaccg tgaaaaatct ttctattgat tatgatccca
    18661 ccaccgatac cggtactaat ggttaaatag accatatgat cggctgcttg gccggcccca
    18721 aactgatttt cgcctaaagc ggccgcattt gcatcatttt ctaaaattac ctctatacct
    18781 gtaatctctt ctaagatttc cacaatcggc acattagtcc aggccaggtt agaattttcg
    18841 atgataactc ccttatttaa atctaaagga ccgggagaac caaccccgat tctttttacc
    18901 tcttttttac taatcttact attttcaatc acgcgttcaa tgctgtcctc aatatttgag
    18961 attattacgt cttcaccctt atcggcttcc gttgggtttt gagctttatt tattatctta
    19021 ccttcactat cggccagggc cgagagaacc ttggtcccac cgatatcaat tcctatatag
    19081 taatctttac tcatctctat tcatctcctc tatctctaaa ttatcatatt cagaggaaac
    19141 agcaatcgtt ccattcaaca tcattaaaga caagaagttc attaaaagga ctgccatacc
    19201 gaacccggtc acaattaaaa tggcggagat tatgaagact attataaacc aaagaaatga
    19261 aaagaccaca ttatcaatcg tcactaaaaa ggaatttttg atgacatacc taatcttctt
    19321 ttctttatca agggctaaaa agccccagta gtaacactgc atcattgaaa aaaagatgag
    19381 gatatataag actaaaacag ccagtacaag catgaaaagg ctgtctgtac ctttttgtaa
    19441 aaagaaaacc agatctgcta ttaagagtag gtatataaaa aaagtaaaga gaaaacctag
    19501 cagtcctctc ttgaaattat cctttatgta tataaaatac tgtttaatag aaaagtcatt
    19561 aaactctatg taattgatcc cgctcatcag tccacccata aaaaatggtc ccagaaaaat
    19621 aatgggaaca attaataagt agtagaactt tataattaaa cctgaatatc cgataaagac
    19681 taccagggta gaaagcatga accaaataag attcaaaata gcaaatttaa ataatttatc
    19741 ataggtttgg gatgtcgagt cttttaacac ctgccatatc ttcaaagtta attcctccct
    19801 gatttgaaaa attcaaattt attatatcac taatgaaatt taatgtaaag aaaaggaaaa
    19861 gcccctgcaa gaggggcttt aattacttgt atttaagatt ttaccttttc tttcttcttt
    19921 tctttttctt tctctttttt actgactctc ttttcatgta tcggtcggga aactttctcc
    19981 cagcgttctg ctaggtcttc taacttttta cctacttctt cagttattac attgccggca
    20041 ccggcatgag taggttcagc acccgactct tttactatct cccttaaagc aggattattg
    20101 tcaataattg gacagggcct taatgaattt tcattaaagg gctgtctctt ttgatattct
    20161 ctaaataagg ggttatctag tacttctttt aacgacttat ccttaatatt atcggtggca
    20221 aaatgggcaa aggcacaggg ttcaacatct ccctttgcat taatatgaaa gtaccttctt
    20281 cctcctgcga tacagccacc tgcataggtg ccatcattcc agaaatcaat taaaaagagc
    20341 ggtttattgg acctaagttc attaactcgc tctgctagaa aacctctttg ttcaggtgtt
    20401 aacatcatat cgaggtcagg gtctctcccg atcggcatat agtggaaaag ccaaccataa
    20461 gttgctcctt tttcgattaa gaaatcataa aagtcctcac cctgaaatag gtcatagaca
    20521 ttattacgat gggcggtaag gctcactccg aatattatac ctctttcccg caaacggtcc
    20581 atagccgcca ttatcttatc gaagacccct tcaccgcggc gggcatcggt ctgctcttta
    20641 tatccctcta aactaattgc gggtgttatg ttacctagtt ccagcatctt atcggccatc
    20701 tcatcatcta ttaaagtccc attggtatac atcataaaac caacatcagg gtgtctttca
    20761 accagttcca tcagacgggg ataaacggtc ggttctcccc ctgaaaagat tataaaatag
    20821 atacccagtt cttttgcttc attgataatc cggtccatgg tttcgaattc aagggtatca
    20881 ctcttatcat attcaccagc ccagcagcct tcacagttta agttacaggc actggtcggg
    20941 tccatcaaga tagcccaggg aactccgtgt cccaatttat ctgccatctt gtactggtaa
    21001 ggtataccca atagggctga attaacaaaa aagtttataa gtaagccttc tttatagctg
    21061 ggggctacat catccaactt gcggatgaat tccttaatta caggattttt cttgtagttc
    21121 tccctgatta cttcaatctg tctatcctgg tttttagtat ttgataattt ttctgccagg
    21181 ttaagtatct ttaaaaagtt ctcttccggg tctttttcaa tatactcaag acccttctta
    21241 actaacttgt ctgcagcaaa tttctttgca aaattgaaag tagccatctc aatccctcct
    21301 tggtattata aaaactgact agtcagtact attaccttct atacttaatg ataacacttt
    21361 tgatatggtt cttcaactca atatcaattt atcaagtatc tctgccacac catcctcttc
    21421 atgatgaggg gcaatcaaat cagctttctc tttgactgct tcagcggcat tatccatggc
    21481 aataccagtt cctgcataac taatcatttc tatatcatta taactgtcac ctatagctaa
    21541 aatttctttt tgttcaattt gttttacctg gcttatctgc tggagggtct ttcctttatt
    21601 aacaccttta gctcctagtt cgatgaagta gtgtttagac tcagtaacat caatctgttc
    21661 tccgtactta tctcgcaaat atttttgata ataactgtgt ttgtcaggat catcttcaat
    21721 aattaataat tttgtagggt catcaaaatc catttcagct agattatcta cctggagggc
    21781 tttgataccg gatacttctt catacctctt tttatattca ttatcctggt caacatacag
    21841 ctggtcattt tgataaaggt tgatatgaag tccctcattt ttaacatctc taatgatttt
    21901 gcgagcaata tctttcttga tcggtttatg gtatttgatt tggccggact taatctcctt
    21961 taccattgcc ccattatata caatcgcttc attatctagg gacagttctt taatataagg
    22021 ttcagccgaa atatacatgc ggccggtagc aataagaact tcaatacctt ttgcctgtac
    22081 ctgtttaatg acctctttgt tcttagcact tatccggtgt tcattattta aaagtgtatt
    22141 atctaaatcg atcacaatta atttatacaa aataactaca tcctatcttt ttattagata
    22201 aacgtattga ggggcgaacc cctcaatatc ataatcgtac tcctatatta attttaatcc
    22261 atattggtaa atatttcaac atatttaacc agaaaactca gccgatagtt catatacctt
    22321 cttctgtaaa tctttcaatt tttcatcatc gtcaatatac tttaagcatt caaagatata
    22381 ttcaccgata agttccattt cttcttcctc catacccctg gttgtaaccg ccggagtacc
    22441 gattcttatc ccactggtta caaaaggact tctggtttca aatggaatcg tatttttgtt
    22501 taccgtaatt ccgaccttat cgagggcggt ttcagcttct ttaccggtaa tatctgtatt
    22561 atttaaatca actaacaaga ggtggttatc tgttccacct gagaccagcc ttaatccgaa
    22621 gctctgtaag gacttggcta aggcctgggc attatctata atctgttttt ggtattcttt
    22681 aaatccatcg gtcatagcct ctttaaaacc aactgctttc gccgctatga tatgcattaa
    22741 aggtccaccc tgaataccgg ggaagatcgc tttatcaata tccttggcat gttcttcttt
    22801 acagagtatt aatccacccc tggtacctct taaagtctta tgagtagtag tagtgacaat
    22861 atcggcatgt tccaccggat tgggatggta tccagctgct actaaaccgg caatatgggc
    22921 catgtctacc atcaagaggg cacccacttc atcagcaatc tgtttaaatc ttttaaagtt
    22981 tattacccgc gggtaggcac ttgccccggc cacgattaag gttggttcat gttttttagc
    23041 cagtttttct acctgatcat agtcaataat ttctctatct tttgtaacac cataatgaat
    23101 aaagttaaag tatttaccgg acatattgac aggattacca tgggttaagt gaccgccgtg
    23161 ggtaaggtcc atcgccaaaa tggtctctcc aatgggtacc ttggcaaaat aaactgcctg
    23221 gttggcctga gagccggaat gaggttgtac atttgcatgg tctgccccaa agattttttt
    23281 ggccctttca atcgctaagt cttctacctt atcaacctgt tcacatccac cataatatct
    23341 tttatgagga taaccttctg catacttgtt ggttaacttt gaaccggctg cttccaatac
    23401 tgcatcactg acaaagtttt ctgaagctat caattcaatg tgtttggcct gtctttcttt
    23461 ttctaattca atgaattctg caatttttgg atctacttct tttagagaac tcaaaatatc
    23521 aaccccttcg ttatttatat ataaaagtta cattaactcc aatttattta ttctctatgt
    23581 aaggattaaa tcctgcttta aatatatatg ttattaaata taatttaaaa atttgcaatt
    23641 atatttaaat attaataaaa ctaaaaccgg agtaattaaa ctccggttat tatatataat
    23701 tatatataat tatacttatg tttattcagg actgattgcg tccacaggac atacttcagc
    23761 acaagcacca caatcaatac agaggtcagc gtcgattaca tattgagtat caccttcact
    23821 gatagcatct actgggcatt ctggttcaca tgcaccacac atgatacatt catctgaaat
    23881 aacatatgcc ataactaaac ttcacctcct cgaatatgta tgaaataata attattactt
    23941 ttaaagtaat tatatatata gtgagtttga ttgtcaaaaa gtattaggta gaaataagaa
    24001 tatatttgct gatcttttgt tcatcttcaa ataaagcttc aaattttaaa tcattattag
    24061 cgtttttctg aataatttcc agatcactct tatttaacac taccttcttt tttacatgat
    24121 tatccagaag gtctaaaatc tcattaaaca tgactgcttt attttccagt ttatatattt
    24181 ctgtaaatat aggttctatt ccttctttac tattacaata aattaaaata ttgacttcat
    24241 taaactttaa aaagatcaat ttatctactt tatttacctc ttcaagccgt aaactcattg
    24301 tactcagcct tttttaaatt agcttcttct gcaaataatt ggactaactt tttggtgacc
    24361 tgtggtttat ttattacaga cggaccccct gcacagccgc cgtggcaggc catcccttcc
    24421 atgaagtcga actcgctttc cccctctaaa actttatcca gaacttcaat gcattgctct
    24481 aaaccatttc cgttcaccgt ctctaatttg gcctcagtaa gttcctgcat cacttcactt
    24541 aaccccccac tgtaggcaaa ctgacgtccg tgcttagtag catcctggac tgcttcttga
    24601 tcacaatagg cagccaggtt cagatcagcg gccaccatga tacacaatag ttcttcaaaa
    24661 gttaagacgt ggtttactga agtatagaga caggcttccg ctttcttcgc aatacagggg
    24721 ccgataaaaa cagttttatt ctcactttcc tttttaactc tttgagcagt tttaaccatg
    24781 ggagaaaagg tatgagatat attcttgatg agttctttat attcatttat gacaagtttt
    24841 ttaaaagaag gacagcagga gctcaataaa aattcttctt tgtttaattt ttcgcttagt
    24901 tcttttttct cttctaccgc cgttaaatct gcccctaagc ccacttctat taccttatca
    24961 aaaccgatct cttttaaagc actttttata gtagctggtt caaccttggc cccaaactga
    25021 ccgacaaaag atggggcaag taaggcggtt aacttatcat ctttatcctt aagggcccgg
    25081 cagatcttaa ctaattctgt actatcactt atggcaccga agggacaggc ctggatacag
    25141 ctgccgcagt ctatacattt attataatta ataacggcct ttcgttcgct atttgaactt
    25201 aaagcatcta cactacaggc ccgtacacag ggtctaatat tttcattaat cgcaccatag
    25261 ggacatgctt ttttacagcg gccgcactcg atacacttct tttggtcgat aaaagcctta
    25321 ttctggatcg tgataatcgc atcaaccgga cagacattaa cacatttgtg ggccacacag
    25381 ttgcggcagg aatcggttac cgtatacttg tcaatcggac agctgtcaca ggccagttca
    25441 attacattga cagtattcgc acagcagtct tcttcccccc tttttgttaa taagtcggct
    25501 ggtgcttctt gtttatgtaa ctgaggatca aaacctaagg ctagtttgat tctttcttta
    25561 actatcgccc tttctttatg tatacaacat ctgtatcttt catctttttt tcttactatc
    25621 ttatagggaa tgctttgtat tttatcttca atagtacctt ctaaaacctg tctggccagt
    25681 tcgatatata tcttcctctt aatcttgact acttctgaga tgggattggc tgccatttta
    25741 aatcctcctt tggttattgt attttatagc ttggctgaga ctatttttct taatttatcg
    25801 ggagtaatat cctgatatac ttccccattc actctcacta cgggtgcttt ttcacagcgg
    25861 tcatctatac agtgtgaggt tttaaccttt attttacctt cagattctga ctctagtttc
    25921 tgcatatcat ctaaaagttc tctagccccc atcaaataac agggggtacc gacacagact
    25981 tcaattgtta ttttatccat cttttttatc ctccttatta ataccagctt atttctactt
    26041 ctttatttaa cttcttgagg ctcttggcaa ttctattcat aacctgggtt ctaattccta
    26101 attctaaagg taaatttaga ctctggtgtg attcattcat ggcccgtccc actatcatct
    26161 tgatataatc gctggctaag agcatcctca ccaggcgggc agccccatct ttaccctcgg
    26221 gataaagttg gccctgttta tggttagcaa ggtaatcagc tgttttattc aaggtaagca
    26281 gtccttcggt cactaaatcc attcccttaa tcttcatgat gggcggtacc gctttatcat
    26341 ggtattcaaa agtggatttt actttttgat tcagctctcg ggccactatg gaagcggttg
    26401 tccctccact gaccactttc cgacctggac cttctaaaag ttcaaacacc acttcttcat
    26461 ctttttgagg agactgagtg ggaccgctta acatggtaag ggatctctct tcccgaaatc
    26521 ccaaacctaa gacagtgaag tcatcgcccg gttccattag ataataggcg agacagatat
    26581 caaccagctt ttcaactata tctttaagat tatagtagtg ctgcaaaaca tcatcaagat
    26641 gccgggcaaa accctgccag cctaatccaa agtccaaaag ggccccgata ccagcgtgaa
    26701 ttactccatc actgaccata aatagcttgt cgtcgttctc tagtttcact tctgcttctc
    26761 ggaccgtttt tccccacatt tccctttctt gcatctctat ctttttaacc tgcccattcc
    26821 ggactaaaaa tatagagggg gtatctatct ctataatttg ggcctttcca ttcctgttta
    26881 cccttagaac agccagggtt gaataggcga tctctcttac ttcacagatc ggcagggtag
    26941 ccaatactgt ttctattatt tcaattaaag gtaacttttt atcaattaaa cctaccgcta
    27001 ttttagaagt taaggtagac agtatattgg ctttgatacc gctgcccaat ccatctgata
    27061 atacaactat ggtttcttta tcgtttttaa tgatctctac attatctcca cagacttctt
    27121 gtccgcgctt tggaatactt tgataaaatt catctattac tattcccatt acttttcctc
    27181 ctgcatcaat tgagttaact catgaagggc tgatttggtt tcaaccgttg tttcacccaa
    27241 aagtccagca atctcctgga cgatcttcat ctgctggtgg actacgtctt cggccttttc
    27301 tgcagccagc tctttcatct cctggacgcg ctttttctgc ctttcgcgct ctgtaatatc
    27361 tgaaaaaatt cccactatca ccttatattc ctcgattggg aaaatggtct gttctgttat
    27421 taggtcatat tgttcgtact ccactttttt gtgtactatc gaatctttgg tctgccagac
    27481 ctctttaaac aggtgcggat ctatataatc ggctaacttt tgtccgtttt cagttacttt
    27541 atgctctcca aacatttcat cggcaaccgg gttaaattcc tggattacca tctctttatc
    27601 taccacaatt attgcattat gggaagattc gacaataata tgggaaagtg attctgcttt
    27661 tgacttcatg tagggaatac acatattttt ctgtgctaat ccatggtaaa ctgcaattgc
    27721 cttttcccgg caggaatcat aaccacaacc cccacagtta gtttcatctt ccggggtttc
    27781 tttacctatt tcttttaaaa tacttctaat gactgcttca gtcggttctt catactcctt
    27841 ttgcctgttc ttataattcc tttttatatc tacctctgcc aaatattttt ccagactatg
    27901 agaattgtac tttacatcct ttttctctgc atactggcta acctttattt ctttactaac
    27961 cgtactggca gcgttctcga ctcccggtcc gttaatacag ccaccttcac aggccatcag
    28021 ttcaataaac cggggggcaa tttttttctc ttttaagtcc gttaatattc tctgagaatt
    28081 ttgcactcca ttcccggaga tgatttcatt ttgactataa tcggataaaa ggttagaggc
    28141 ccttactgcc ccgttttcca ggggatatgt accacttatt tcaccacagc cttcctcaat
    28201 ttgataactg cagtggtcat ttaattcgat accattttct tcacataatt ttttaaactg
    28261 ataaaatgtg ataacggcat ctaccttaga ttcggttcta tcggactcca tctcatccat
    28321 tttagcgata cagggaccga taaatactac cctgcttttt tctccatact ttgttttaag
    28381 taaggaagca tgcaggttca tcggtgaaac aatatcagcc aagtattctc tcatctccgg
    28441 atagtgtttt tctattaaat taatgacagc cggacagcag ctggagatca aaggcttttt
    28501 tctatcctta atttctttat taaaataatc tgctaccagt tcagccgcct gggccgtctc
    28561 tgtgatcata tctacgccca tcgcccttag agtacctatg atattgtacc actcaatatc
    28621 ttgataggca gttaaatagg aaggggccag gctgacaata actttttcct cattatcaat
    28681 atattttaac agccttccct tcatgtcata tggtttcttc gaattctggg gacagatatt
    28741 aacacaggtc ccacagtaga tacatttgtc ttctatgatc caggcctgtt catcaaaacc
    28801 gatcgcccct acttcacagc ttcttaaaca gcggtgacag tcctgacaac tggactttac
    28861 tacatcaagt actttcattc acaatctctc ctaaaatttt tttattgata ataccctcta
    28921 catcttcagg tgctacatta gccacttttt gactgccaat tgtaatatta acgccctgag
    28981 tacagttatt ctggcagaaa ctggccttta aatttacctt atcttccagt tctttttctc
    29041 caattagacg cttgaattct tctacaactt ccggtgctcc cttgaggtga cagctgctgc
    29101 ctacacatat tttaatttct aacatggttt attctcctta ttatgtgatt attttcacat
    29161 taatcataac ttaaaaaacc ctgcttgtca acagtaaaac agggttttta catgcttctc
    29221 gtattttttt ctaaataatc actgataacc tcagctaatt tgtcgctaat tccttccact
    29281 tcctttaatt cagatcggtc cgccaatctt attttagcca gggaaccgaa atgctgcagt
    29341 agtgcttttt tctttttagg tccaatcccg ggtattctct ccagcatgct gtgagttatc
    29401 cggcgggacc ttaacttccg atggtaatta acagcaaatc gatgggcttc atcccttact
    29461 ctctgcaaga gatggagggc atccgagctt ttcggtaata ttatcggttc ttttctattg
    29521 ggtacaaata cctcttcttc tcttttagcc agaccaatga tctgcatatc tgctaggccc
    29581 agttcctcta atacagtcaa ggccgcattt aactgacctt tgccgccatc aattaaaatg
    29641 aggtcgggca gcttgcggtc ttcctctaaa agtctccggt accggcggtg gaccacttcc
    29701 ttcatcatag cgaagtcatc cggcccttcc ttctgtctta ttttaaaacg gcggtagtcg
    29761 ctcttggagg gacggccgtt tttaaagaca accagagaag caaccgggtc tgtcccctga
    29821 atattggaaa tatcaactcc ttcgatatgg gaaggtaatt catctaattc taaatagtcg
    29881 gccagttttt caactgcttt tacagttctt ttttcttcat acttcttttt aatactttct
    29941 tttttaaggt tctgtttagc attttttaag gccatctcga ttaagcgttt tttattacct
    30001 ttttgcggaa tctttatctt aacctgtttg cctttcagct gttttagtcg gtcagccagt
    30061 atctccctat tatttatctc ggtattgatt aaaatttcat ccggtagttg gggagcctgt
    30121 tcatagtact gctgtaaaaa ggaggacatt acttcccttt cgtcttctcc tcttgtacct
    30181 tttaagataa aatgctcctg tccaatcagt ttaccgtttt ttactaacag caattggaca
    30241 cagtaggagc cgtcttcagt gatcgcgata atatcgcgat ctttattatc ttctgacatc
    30301 accttctgct gtcgggaaat ctcttccacg gcctgcatgg catcccggta gcgggcggcc
    30361 cgttcgaagt tctgttcatc agcagctgcc tccatctcct cttctatctt atttaataaa
    30421 gtcttctgac ggccggccag aaacataatc acctggtcga tctgggcccg gtattcctct
    30481 ttagaaattt taccgacaca gggggccgta catttatcta tatagtagtt caggcagggc
    30541 ctttccagct gattatcaac actgaagtca tagttacagc ttcttaattt aaagagatct
    30601 tttaatatat ttatcgtttt atagattgcg tctacatcgg caaagggtcc gaagtacttg
    30661 ctcccatcct tttttactac ccgcgacttt ttaatgcggg gaaaatcctc ataggtagta
    30721 attttaataa agggataggt cttgtcatcc ttcaatctta cattatactt gggatgatat
    30781 ttttttatca aattagcctc aagtatatat gactccatct cggtatcagt tactatatag
    30841 tcaaagtcat cgatatgatc gatcatgata gaggtcttat agctctggtt tcccctgcgg
    30901 aaataggagc ggacccgctt tcttaaggag cgggccttgc ctacatagat aatctcaccg
    30961 gaatcatctt tcatgagata gacgcccggt ttatccggta tttgtttaag ttgttcttcg
    31021 atatattcac tcataaataa tcaccagtta ttttaagata tcctttaaaa acttgccagt
    31081 ataggagtta tcagcttctg ctacctcttc cggggtacct gttgctaata cttctcctcc
    31141 gttatcgccg ccctctggtc cgaggtcgat aatatagtcg gctgatttta ttacatcaag
    31201 gtcgtgctct atgactatta cagtattacc gccttcccgc agtctgtgta atacttctaa
    31261 tagttttttt acatcggcaa agtggagacc ggttgtcggt tcatcgagca tatatagggt
    31321 ccggccggta cttcttttac cgagctcttc cgatatcttg acccgttgag cctcaccacc
    31381 cgaaagggta gtagcgggct gtcctaattt aatgtatccc aaacctacgt cataaagggt
    31441 ctgtaatctg cgttttaagg ccggtatctt atcgaagaag tttaaggctt cttctaccgt
    31501 catatccagt acatctgcga ttgttttacc cttatacttg atctctaagg tttccttatt
    31561 gtatcgcttg cctccacaga catcacaggt tacgtaaaca tcggctaaaa agtgcatttc
    31621 aatcttgttt ttcccctggc ccttacaggc ttcacaacgc ccgcccttta cattaaagct
    31681 gaacctacct ttatcatagc cgcggcggcg ggcttccggt gtttcggcaa aaaggtccct
    31741 gatatagtca aatacctttg tataggtagc aggattggaa cgcggggtcc ggccgatcgg
    31801 ggactgatcg atgttaatta ccttatcgag gtgttctaaa cccttgactt catcatgttt
    31861 gccgggatgt tcggtcgaat cataaaagtg ctgcatcagt ctccgcttca aggtatagtt
    31921 aattaaagtt gatttaccgg aacccgaaac acctgttata caggtaaagg tccctaaagg
    31981 aatttcaaca tcgatgtctt ttaggttaaa ctgcttggct ccttttattt ctaaatactt
    32041 accgttcggt tcataacgtt catccgggat cggtattttt tcttcacctt tgaggtactt
    32101 accggttaat gatttatcac agtctttaac cttttctaaa tctccctgga caacaatatt
    32161 tccaccgtgt ttaccggcgc ccggcccaat atcaatgata tggtcggccg tcctgatcgt
    32221 ttcttcatca tgttcgacta caattacggt attgccgata tcgcggatgt gttctagggt
    32281 atcgatcaag cgcttgttat ccctctggtg aagtccgata gaaggttcgt ctaagatata
    32341 taggacacct accagaccgg aaccgatctg ggtggcgagc ctgattctct gggcttctcc
    32401 ccccgagagg gtggcagccg aacgttccaa ggtcaaatac tctaaaccga cattgaccat
    32461 aaactgcagt ctatttctga tctccttaag gatctcctgg ccgatgaact tttctctttt
    32521 acttaacttg aggtttttaa taaattcgag ggcctctcct atatttttgc gggtgaattc
    32581 ggcaattgag atcccgccga ctgtaactgc cagagcgcct ggttttaacc tctgtccatt
    32641 acaggcctta cagtcatctt cccgcatata tttttccatc cggctgtggg aactggctga
    32701 atctgatctt ttcatccttc ttttaagata gtttacgacc cctttaaact gggtattctg
    32761 ctgtcttgtc tttccatatc tattggtata gggaaagctt aaggtccggt ctgaaccata
    32821 gagtaatata tcctgaattt cttcatctaa ctcaccgatc ggggtgctca tactaaaccc
    32881 atattctttg gctaaagcct tcaagatctg gggatagtat ttactactgg aattacgcca
    32941 gggtatgata ccaccgtctt gaatagacct ttctttatcc aggatcagtt cgggatcgaa
    33001 ctcttttttg attcccaacc catcacatac ttcacaggct ccataaggac tgttaaaaga
    33061 aaacatacgg ggagaaagtt cttctaagtt aaagccgtgt tcattacagg caaacttttc
    33121 actaaaggtt aaggtttctc cttcaatgat atcaacatag actaagccgt ctgcatactc
    33181 tagggctgtt tcgactgaat cggccagtct ttccctgata tcttccttga tcacaattct
    33241 atctacgatt accgagatat catgcttctt gtttttgtcc agatctattt cgtcatcaac
    33301 cagatagtta tctccatcta ccttgacccg gataaagcca tcccgccggg cctgggcaaa
    33361 tacctggtcg tgttcacctt tccttcccct gataaccggg gctaaaatct gcagttttgt
    33421 cctgttttta agttcaagta cctggtccac tatctcatct atggtctggg aacttatctc
    33481 ttttccacag atcggacagt gcggggtccc gattcgggca taaagtaacc ttaagtaatc
    33541 atagatttca gttactgtac ccacggttga acggggattt ttactggtag atttttgatc
    33601 tatcgagatg gccggggaaa gaccttctat atattcaact tttggttttt ccatctggcc
    33661 taaaaactgg cgagcatagg aagaaagtga ttctacatac ctcctctgcc cttctgcata
    33721 aatcgtatca aaggcaaggg aagacttgcc agaaccactc agtcccgtta ttaccaccag
    33781 ttcatcgcgc ggtatatcta catccaagtt ctttaaattg tgttcacggg ctcctttaac
    33841 agtaatcttt ttgctgccca ctttaatcac ctcatctatt tgaaagttct tctttgattt
    33901 cttcgatttc atctctgatt tgtgctgcta cttcaaactc cagatttgag gcagcctcat
    33961 ccatttcttc ttctaattca ataatcttat tctgcagttc gatctttgat agttctgcaa
    34021 aattgtcttc gtcatctgta ctatatacct tttctttact atcttcaata actaattttt
    34081 ccggtcggag aacttccctg accggttttc tgatcgtctg tggttctata ccatgttttt
    34141 cattaaattc tttttgaatc tctcttcttc tttccgtctc atcaatagct ttccgcattg
    34201 aatccgtaat cttatctcca tacatgacca ctttaccatt cacattccgg gctgccctac
    34261 cgatggtctg aatcagaggt ctttctgccc gtaaaaaacc ttctttatca gcatctaaaa
    34321 tggctaccag ggaaacttcc gggatatcaa gtccctctct caacagatta attccaacca
    34381 ggacgtcaaa ctctcccagt cttaaatctc taattatctc cgatctttcc agggtatcta
    34441 tatcagaatg gaggtaacgg actctaatac ctgcttcacc caaatattct gttaaatctt
    34501 cggccatcct tttggttaaa gtagtaacca ggactttttc atttctttct tctacaacta
    34561 cccgaatttc ctccaggaga tcatcaattt ggccttcaac cggttttact tctaccttgg
    34621 ggtcaataag tccggtcggc cggataatct gttctacgat ctgctggctg tgttcatgtt
    34681 cataaggtcc tggggtagcc gaaacataga ttgcctgtgg tactacttct tcaaactctg
    34741 caaagttaag cggcctgtta tctaaagcag aaggcagcct aaaaccatac tccactaatt
    34801 tctcttttct cgatctatca cccgcaaaca tccccccgat ctgaggtatt gtcatatggg
    34861 attcatcgat aaagactaaa aaatcatccg ggaaatagtc aagcaaggtc tggggcctgg
    34921 aacccggtgg acgtccgctt aaatgtctgg aatagttttc aatacctgaa caaaagccca
    34981 tctgttctaa catctccaga tcatagtttg tcctctgttc taagcgctgg gcttcaacta
    35041 atttatcctg ttctctaagt tcttctaatc tttcgaccag ttcttccttt atcgttttaa
    35101 tagcgcgttc gatcttatct tccggggtca cgaagtgact ggccgggtag atagttattt
    35161 catccatatc ttccataatc tcaccggtca ccgtattgat ccgggtgatc cgctcaattt
    35221 catcaccaaa tagttcaacc ctaatcgcct gatcgcggta agcgggatag atttctaata
    35281 catccccccg aacgcggaag tgaccgcgct gtaagtcgat atcatttcgg ctgtactgca
    35341 tcatggaaag ttctctcatg atctctttgc gcgatttttc tttccccacc ttaagatgag
    35401 aggaaagctc caggtaatca gcgggagaac ccaaaccata tatacacgaa acactggcca
    35461 caatcagaac atctcttctt tcaaagagag cactggtggc ggccagtctt agtttgtcta
    35521 tatcctcatt aattgaagca tctttctcaa tataggtatc agtctggggt acataagctt
    35581 ccggctggta gtagtcgtaa tagcttacaa agtattcaac ccggttttcc gggaagaact
    35641 ctttaaactc actgaccagc tgggcagcca atgtcttatt atgggcaatg acaagagtgg
    35701 gtttttgtac ttcttccaca acacccgcca tcgtatatgt cttacccgta ccggttgcac
    35761 ccagtagggt ttgatgtctg taccctttgg ctactccttc cgccaactct tcaattgcct
    35821 tgggttggtc acctttcggt tcataaggag aatgaagttt aaattctttc ggcataaata
    35881 ctcacctttg tttaaatatt tttcctgtag acattatact atatataccc actatgtgca
    35941 aacaaatgtt catttagtcg cgggttttaa acattttaac atgatacaat taattgtaat
    36001 agtaattatt actaaaaaaa gttaaaaata tatattattc ttttttgtta aaaatcttgc
    36061 agttcctcgg ctattttttg tccggtctcg gttttagcta aaccggcctc cgaggtttct
    36121 tttaagctaa ccggcatgag gtctccaatt tccatcatgg ctgcaatgac ttcatcggga
    36181 ggtattacac tactgatccc gcttaaggcc atattaaccg ctgtcagggc gtgggaagcg
    36241 gcaaatccat tccttttaac acagggaacc tcaaccagtc ctgccaccgg atcacagact
    36301 aaacctaata gattttttaa ggctaaggca aaggcatctc caacttgttg gggagtgccg
    36361 cccattagtt cgacagctgc acccgctgcc atagcggaac ctaccccgca ttcagcctga
    36421 cagccgccgg ctgctccggc caatgtggcc tttttggaaa ctacttctcc cagccctccg
    36481 gctgtaaaaa gggcatctat cagcttatct tcactaattt catgttcttc tgttagagtg
    36541 attaaaacag ctggtacgat accactggca cctgctgtcg gtcccgctac tatcttaccc
    36601 atacaggcat ttacttctga tacagccaga gctttaataa ttaccttctt gtataaaggg
    36661 gtgtttaaag atttattgga attctgtaag gttaacgctt cgcccccggt taaacccgta
    36721 gcagacttat gacccggagt tagaccttct gaaatagatt ccctcattat ttttagatgt
    36781 tttttcatct catctatgac ggtttcccgc tcaatctccc tttcataaat ctcttcttct
    36841 ataacgatct ggcttatctt cttattttcc tgttcagctt tatcgattaa ttccttaatc
    36901 ttattatact gcattaacaa gtcctcctta aaaaggctct atataccgca cctggtaaat
    36961 attgtccata ttttgcaggt gagaaattac ataatcactt accttatggt ctaattcaat
    37021 gatggcagat cccacttctc cctttttatt tctaaatacc tccatactgg caatatttaa
    37081 cttgtagctg ccgagaattg aggttattat tcctatctca ccgggttcat ccttatgcaa
    37141 gacccagagg gtcggcatct caccggaaaa gtcgacctca taatcatcga tttggattat
    37201 cttgatccgg ctgcccccaa ttgaagaacc aatgatggtc atctccttgt tttcaccgaa
    37261 aagttctaac ttggctgtat tgggatgcac ccctcttaag tcagaaaggt gcaattcgat
    37321 ctcaactccc ctttctttag ccaggttcag ggaatcacgg atcctttcat catctggttt
    37381 aaaacctaac agaccgccca ctaaggcgcg gtctgttcca tgtcctttaa aggtcctggc
    37441 aaaagatccg tgcagatata tatcagcttt aactaattta cctttgaaaa tagagcgggc
    37501 cagataaccg actttaactg cccccgccgt atgggagctg gaaggcccga tcatgattgg
    37561 tcctaataca ttaaatacgc tcacttactt tatccacctt tctagcttgc tttatccagt
    37621 gatttggtcc ttactttatc ttttaataga tcaattgcct tttgtagttg ggcatcttct
    37681 tcactttcct ggtcaaattc aaccttgata tcgggttcta tccctttttc atggataaag
    37741 gtaccattgg gtgtataata tctggctgtt gttaaccgca gggcagaacc atcacctaaa
    37801 ggaataacgg tctggacagt acctttacca aaggtctttt gtccaactag cttacctcta
    37861 ccatagtccg aaatggctcc cgctactatt tcagaagcac tggcactacc ttcatttatt
    37921 aatacaacca tcggcatatc aacagcctct atatcagaga cggtattata tacttcatct
    37981 tcttttcttc tgcttttgac ggttactacg gttccatctt caataaagtt actggccacc
    38041 ttaactgcct catctaagat tccgccgggg ttagttctaa ggtcaagaat tagagcttca
    38101 gccccctgtt tctttaaatc ggtaagagcc tcttcagtct tgtatcccac ctcactgcca
    38161 aactgataaa tcgataaata gccaatggtg tctgattcca tattgtgttc cacatagggg
    38221 atctctacat tggcccttgt aatatccaca tcaaaatctt cttctttatc ttcgcgtgag
    38281 atagtcaaag taacgtgagt gcctggttca cctctcatta ggtcgacagc tttttgttgt
    38341 gacatctttg aagttaactt cccgttaata gctttaatta catctcctga tttaagaccg
    38401 gctctatcgc cgggagtatt cttaatcggg gaaacaatgg ttaacttctc ttctctgacg
    38461 gttatgacaa tacctattcc gccaaattca ccatcgaatt caatctgcat ctcctcatat
    38521 tcatctggac ttagatagga tgaataaggg tcaagctctt ttaacatacc tgtaattgca
    38581 gaatcaatca gatcttctaa gtttttatct tctacataat agttatctat aagataaatt
    38641 acattttgta aatttttcaa cggagttaga tcatctgcaa ttactttaaa accaccgact
    38701 gttaaaattg ttatgacaag taatacagct aataacttct tcttatttct aaacattttt
    38761 tatctcttcc ttccaatctt ttatgatctt aaatatcgta tttttctttg actactaaaa
    38821 acaaaaaccg gggtaaagcc atcattcttt taatccgctt cggctcatgt aaaagccggt
    38881 aaaaccattc taaatacatc ttctgcatcc agagaggagc tctttttgac tgaccggaaa
    38941 ggacgtcaaa acttcctccc accgttaatc caatctgtac atccaagtcc tcaaaatggg
    39001 agtcaaaaaa cttctcctgt aaagggactc ccatccccac taacaatata tcgggtttct
    39061 tattattaat ctcttcaata atcatttctt ttgaatcaga atctaaataa ccgtggtgaa
    39121 aaccagccat actaagctca ggtagttctt ctcttatgtt ttgcacagct gagtctatta
    39181 tacctggttt ccctcctaaa aagtagaccg aaatatcttt atcttctatc tcattaagca
    39241 gctttctcat taaatcataa ccggctaccc tttcctgtaa gggttcacct aaaagtcggg
    39301 ctgctaaaag gaggccggcc ccatccggta ccaaaaagtc cgctcggttt aatagtttag
    39361 agaaacttgt gtcatcataa gcgcggactg caatttcaga attgggtgta tagattagtt
    39421 ttttggcttc tttgttatag attgtattta ttaattcact taccatctgc tgcatcgtta
    39481 tattatttat cttaagggtc agtatttcgg ttttatcctt caacttattc acctttcaaa
    39541 tcagttaata atctgctaat tgaacctttg actctctcat ttttgacctt tacaatcctg
    39601 tctattctat ctttaatttc ttttttttca tctttaatta tatcataaga tttaattaat
    39661 tgctcttcag taatattatc ggtcgtgata ttaggctggt agttcatctc ctggattaaa
    39721 ctctcaacct tgggatcata acttattccg ataaatggta cacagtttat cgcagcaaat
    39781 atcagggaat gaagcctgac ccctataaaa acatcgaaag tgctatacaa attatttatc
    39841 tttttcatat catcagtata atcaattatc tcagcctggg tatatagcat attttttagc
    39901 tgttcactaa ctctttcatc ttccccttcg tgaaagggaa agatggtaac cgtgtactct
    39961 tcttttttga tcaggtagtt taaaaattca gccacttttt gcagatagga attatcttgc
    40021 cagctgcgga ctgaaacccc gatcttttta gtgttagaaa cttctgaatc actaccttta
    40081 cagatattta acccatagac cgggtcatct atcagtttta tcttatttcg ctcgataccg
    40141 atttcttcta aaaacagctt ggaatttaaa tcacggaccg aaagaaagtc cactctactt
    40201 aaaacctttt tgatcaaaaa cctgtaaaaa ctattgttaa caggtccaac tccctgggca
    40261 aaaaagaccg tttttagatt aaaaacctgg gctaaaaata ttaatcctaa ataatagggg
    40321 actgaacgaa ggctggttac atcctgcagt aagctgcctc ccccactgat aaagatatct
    40381 gcttttttca tggcggctgc tattttaaat atatcatatc tataaatagt ttcagtatta
    40441 tacttaacct ttgtttcagc tgtattttgg gtaagtacag ttagctcggc ctcatcggtt
    40501 tctgccctta tcatatcaac catactggcc agaatagctt catcacccag attattaaaa
    40561 ccgaaatagc cggaaagcag aattttcatc aaaaatgtag caaggaaact ccatctaaat
    40621 cttgtcttta atttaggtgg agaggaattg ctacgttctt caccttcctt tccatttttt
    40681 tctatatttt cttgataata ctaattatgt aagatataat tagtatagaa ttgaaaatcc
    40741 taaatttaaa ctaaaaactg ccaaattata taagtataag ggccagtggt atctcgactt
    40801 aattatggaa tacgaaaaat ccaaaaatga aacttataat tatataggtg ttgattttgg
    40861 tatcgttaaa ctggccacct ttagtgtgct gaattacaag caagatatca tacaccagga
    40921 atgtatttca ggtagtaagc tgcgatttat acgta
//
LOCUS       DATGCJ010000054        11229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_140593, whole genome shotgun sequence.
ACCESSION   DATGCJ010000054 DATGCJ010000000
VERSION     DATGCJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140593"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VKN64_02655"
     CDS             complement(<1..450)
                     /locus_tag="VKN64_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA59166.1"
                     /translation="MLKDIKFLITFLIAFILLFSFNSISLQARPLLFAPEEKVEIYCA
                     WNDDFNCLAKSKLYELLKNKYPNVEIINAHIACTNKDDINDLLIKRIFEGNPPDIFQV
                     HCSHELINNLVKTGSVSSITNLLNEMDIIKKFNPQILRMYSYEGEVYA"
     gene            779..1924
                     /locus_tag="VKN64_02660"
     CDS             779..1924
                     /locus_tag="VKN64_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HMA59167.1"
                     /translation="MSQPTSFDIMKNRNEKLILKIIKENQQISRADIADKTGLSPATV
                     TNIVNDLIENQLIKESKRGKSKGGRRPVYLKLNGEEIKVIGINWRINSISYALVNLEG
                     DIEIKGKKTINNLVLGNFLKKTRIITRELFKQVDQNKVKGLGVGIHGVVDPIKGVSRF
                     APHFKWKDKNILEKLNKIYDLPIRIDNDVRMMARAERWNKKKNFVYINTGRGIGSAVV
                     LNNRLIYGKDFLAGEIGHIIVKEEGPLCSCGNRGCLEALVARKNLLEYSDKVFGINID
                     SWSKLSNIINSNEKDTELILKYISKYFGLAIVNIVNLLNPNRIIVGGDFLDIKDKLSP
                     YLSSFVVDNSLNKESSPIEFTEFNKIAGAAGAAFSILEKYFYNEEEI"
     gene            1924..2904
                     /gene="galE"
                     /locus_tag="VKN64_02665"
     CDS             1924..2904
                     /gene="galE"
                     /locus_tag="VKN64_02665"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMA59168.1"
                     /translation="MSVLVTGGAGYIGSHVVKDLVENDFDVITVDNLQKGHKKAVLGG
                     KLIVGDIKDTEFLQEVFSYNKIEAVVHLAADSLVGESMEDPGKYYHNNLDNGIKLLDV
                     MLENGVKNIVFSSTAAVYGEPEEVPIKEDHPIDPGNPYGESKYFFEKVMERYDQLFDL
                     NYISLRYFNAAGADPSGQIGEDHDPETHLIPLVLDTALGKRDKLYIFGTDYETRDGTC
                     VRDYIHVNDLASAHTLALQALMQGKESSIFNLGNGEGYTVKEVIETARQVTGEIIPAE
                     NSERRAGDPAVLIASSKKIQKELNWQAEYPDLETIIKTAWNWHKKGGFKQ"
     gene            3012..4001
                     /gene="galT"
                     /locus_tag="VKN64_02670"
     CDS             3012..4001
                     /gene="galT"
                     /locus_tag="VKN64_02670"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HMA59169.1"
                     /translation="MSEMRWNPILKEWLITATHRQDRTFNPPEDYCPLCPTEPGGFET
                     EVPRPDYDLVVFENKFPSLERAPREPVVEGTELTPVTDSKGICEVVLFTSDHEGVMSE
                     KPLSRFIKLIKVWKDRYEDLSSKEFIDYVYIFENKGEEVGVTLSHPHGQIYAYPDIPP
                     LIEKELKNSKEHLEKEGSCLYCDVLKQEIEQEERIIVQNDSFTALIPFFARYAYEVNI
                     YANEHITSFAEFNEKLIKDLAGVLKTLIKKYDNLFGFEFPYIMAIHQKPAVEGTEDYS
                     HFHIEFYPPYRTKDKLKYLAGSESGAGAFINGSLPEEKAEELRNTEPFEICEE"
     gene            4011..5174
                     /locus_tag="VKN64_02675"
     CDS             4011..5174
                     /locus_tag="VKN64_02675"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HMA59170.1"
                     /translation="MEKSILKEHYKNNFKNTKKRDGFFFAPGRVNLIGEHTDYNNGFV
                     LPMAIDRRIYAYLQVRDDKQINIYSIDLDRYYTTTTEKLKFNEENSWSNYIVGVVSEL
                     QKEGYHPSGFNMTFSGDIPQGAGLSSSAALEVVTVYGLAELNNFEISPVKKALIAQSA
                     ENNFVGVECGIMDQYISCLGEKDKALLIDCDSYDYNKVPIQNKKYQFLIIDSKVQRGL
                     VDSEYNLRRQQCNNVVKYFKENFAEDIESLRDININELFRHEDKINETDYKRATHVLT
                     ENRRVLEFADSVEDGNYEKAGQLMYASHLSLKNEYEVSCEELDFLIETALELKGVLGA
                     RMTGAGFGGCTVNLIKKDYKSKIINSIKTEYKKEYKKEPDFYTSRPAQGASSY"
     gene            5256..6614
                     /locus_tag="VKN64_02680"
     CDS             5256..6614
                     /locus_tag="VKN64_02680"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HMA59171.1"
                     /translation="MTEYKFPEDFTWGAAAASYQIEGAYDEDGKGESIWDRFTHQKGN
                     IKNGDTGDVACDHYHRYKEDVKLMKKIGLDSYRFSIAWPRIFPEGTGSINQKGLDFYK
                     RLVDELLQNGIKPLPTLYHWDLPQALQERGGWENRDTAKYFHDYAMRIIEEFDGVIDK
                     WITHNEPWVVSFVGHAFGQHAPGKQDYQSALQVLHHLLLSHGMVARTFREEEMNGEIG
                     ITVNLSDFQPATDKKEDLAAAWRQDNFLNKIVLDPLFRAKYPKELFEFFEENVGEIKV
                     REGDMSVISYPMDFLGINYYSRNVIEHAEEANILESKEVKIEDSQYTEMGWEVYPQGL
                     YNVLNRVNNEYSDLPLYITENGAAFKDKLTEDGKVKDDKRVNYLKNHLLKAQQAIEDG
                     IPLQGYYVWSIMDNFEWGYGFSKRFGITFVDYKDNQKRYIKKSGKFYSEVIERNGIEI
                     KD"
     gene            6742..7749
                     /locus_tag="VKN64_02685"
     CDS             6742..7749
                     /locus_tag="VKN64_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA59172.1"
                     /translation="MSTTLKDIAKKANVSVATASLALNNSEKVNHKTYEKIKKIAEEL
                     NYTPNARARALVKKATETIGLVIPEVENPFFAEMAQSIKNMIKESGYNLILCSTEYKS
                     EEELRYINLFKSGLVDGAIFACLGDMMEEHNKMIIDLVKNNTPVVFVDREINNYNLIP
                     VIKTDLFEGAYNIATYLINNGHKKIGFVGQSEERVKGYKKALKDNNIKINQDFIYYDF
                     LTIEGGYKVGQKIVTLDELPTALICLNDEMAMGVIQSLTSADIDVPDDISIIGIDNIR
                     MSEYYNPSLSTLHIPKKEMGKKAAEVLLKRIKKERLSSDEQLIIFPTELIVRKSSKRL
                     N"
     gene            7837..9078
                     /locus_tag="VKN64_02690"
     CDS             7837..9078
                     /locus_tag="VKN64_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMA59173.1"
                     /translation="MKIKKLLPVLLVLTLLFAFSAVTMAETLTVNTYTSDPAPRKAMK
                     RLVEIYEENNPDIDVQLNITAHEDFKKAIRVWLSSDNPPDVFSWFAGNRAKYFVDKDL
                     VMPFTDVWQEENLDEKIPRAFKSISFFEDEAYFLPHSYYWWAIYYRESIFEKYDLEEP
                     QTWEQLLEVGETLKENGITPFAIGTKFKWTAAGWFDYLNMRINGPEFHVDLMHGEVPF
                     TDDRVAEVFKKWKYLIENDFFVEDAAAYSWQEGLQFMTQGDAAMYLMGQFINDAVSED
                     VQKDMNFFRFPIINKNVPIGEDLPTDGFMIPAKAPNPELAKDFIAFLASVEGQRVFAE
                     DMGRMVPNSDVPDEVYDEPALKGKEMIENTDALAQFYDRDMDPAMAGDTMDLLMKFWQ
                     YPDQYESIMEEMEQVRQKVME"
     gene            9152..10021
                     /locus_tag="VKN64_02695"
     CDS             9152..10021
                     /locus_tag="VKN64_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59174.1"
                     /translation="MKKRKVQWRFLAIPLIFYTIWVIGPTIYTFYLSLTEWDGLTDPS
                     FIGITNYLDLFNDSVFYTSLVNNFIWLFIFLLIPVVLGLFLAILLSKKIRWAKGFKAA
                     IYSPMILAPVVIGLIWSWIYHPKAGILNNTLTYVGLDFLTRGWLSDPETALYAVIGAA
                     VWRHTGYVMLLFLAGLTSIPPTLIEAARIDGATAWQRFRYIILPLLKPATVIVLVITM
                     IQSLRAFDFVNIMTRGGPFNSTNVLANYMYTEAFRNYEMGYGAAIAVILFIIMFVFIF
                     FYLRRIREEELNV"
     gene            10018..10854
                     /locus_tag="VKN64_02700"
     CDS             10018..10854
                     /locus_tag="VKN64_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59175.1"
                     /translation="MNYLKKRKYLNAFIFIALLLFVIAWLLPVFGAFLTAFRSMDEIM
                     DKGFWTMPEKLSFDNFKVAWSEGNLNTYLLNSFIITIPSLLGTLFLSSLAAYALAQYD
                     FKLNMPLYLMFVGGMLIPFQILLIPVFKLSNFLGIYNTYWGLIMIHMVFQIGFCTFFL
                     RNFMMTIPEELSEAARMDGCSEFKIFYRIYLPLSKPALAALGTLEFTWIFNDYIWALV
                     LIRDNKFKPVTAGLASLKGNFITSWGVLVAGSLLATIPTIIVFIFLQKYFIRGLTMGT
                     GK"
     gene            10877..>11229
                     /locus_tag="VKN64_02705"
     CDS             10877..>11229
                     /locus_tag="VKN64_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HMA59176.1"
                     /translation="MRQILNFNTEWQYLPEDNKKMKDISYSNSKIEDISIPHSNKLVP
                     HHYFNHTEYEFISWYRRPFYLDESYEDKKILVKFDGIMKKAEVFVNGTLVGTHKGGYT
                     SFKLDITDYVNINEN"
BASE COUNT         3883 a         1730 c         2256 g         3360 t
ORIGIN      
        1 ggcataaact tctccctcat aactgtacat tcttaatatt tgcggattga atttttttat
       61 tatatccatt tcatttaata aatttgttat tgaagaaaca gaacctgttt taacaaggtt
      121 gttaatcagt tcatgactac agtgaacctg aaagatatcg ggtggatttc cctcaaatat
      181 acgttttatc aaaagatcat taatatcatc tttattagta caggcaatat gtgcatttat
      241 aatttctaca ttcgggtact tattttttaa gagttcatat agtttagatt ttgctaagca
      301 gttaaaatca tcattccagg cacagtatat ttcaactttt tcttctggtg caaaaagcaa
      361 gggacgggct tgtaaagaga tgctgttaaa ggaaaagagt aaaataaaag caattagaaa
      421 tgttattaaa aactttatat ctttcaacat ttaaagatcc ccctttcatc tataaatgcg
      481 aacaactatt taattattaa atatgcaagt aattgatttt aaagaaagga ggtttaaatg
      541 aattagaaaa ggggaaaaag gttagaaaaa agaggagtgt tcaactatca aatgtgacaa
      601 agaataattt aaaagacctc ataaacagtt aagtgaagta taaaagcatc tacaccataa
      661 ttatacaact aaattcatat ttatgcaatg aatttaaaca atataatttt acaaaagaca
      721 aaaagtatga tactctttag ttagttaatt tattaaataa agaaagttgg tgaataaaat
      781 gtcccaaccg acaagttttg acataatgaa aaaccggaac gaaaaattaa ttttaaaaat
      841 tataaaagaa aatcaacaaa tttcccgggc tgatattgct gataagacag gtctttcacc
      901 tgccactgta actaacatag ttaatgattt aatagaaaac cagctaatta aagaaagcaa
      961 acgtggtaaa tcaaaagggg gaagacggcc tgtttatctt aaacttaatg gggaagagat
     1021 taaggtgatt ggtattaatt ggcgaataaa ctcaatcagt tatgcccttg ttaatttgga
     1081 aggagatatt gaaataaaag gcaaaaagac gataaacaat ttagttttag gaaatttctt
     1141 aaaaaagaca aggataatta ccagggagct ttttaaacaa gtagatcaaa acaaggtcaa
     1201 aggtttagga gttggtattc acggagttgt agatcctatt aaaggagttt ccagatttgc
     1261 tcctcacttc aagtggaaag ataaaaacat tttagaaaaa ctaaataaaa tatatgattt
     1321 gccgattagg attgataatg atgtccggat gatggctaga gcggaaagat ggaataaaaa
     1381 gaaaaacttc gtatatataa ataccggccg gggaatcggt tctgccgttg ttttaaataa
     1441 tcgcttaata tacggtaaag attttctggc aggcgaaatt ggccatataa ttgtaaaaga
     1501 agagggacca ctctgcagct gtggaaatag aggttgttta gaggctttag tagccaggaa
     1561 aaatttattg gagtattctg ataaagtctt tggaatcaat attgatagct ggagtaaatt
     1621 aagtaatata ataaattcga atgagaagga tacagaactc atcttaaagt acatcagtaa
     1681 gtattttggg ttggcaatag taaatattgt caatttatta aacccgaata gaattattgt
     1741 agggggagat ttcttagata ttaaagacaa actatcaccc tatttatctt cctttgtagt
     1801 ggataattca ttgaataaag aatcctctcc catagagttt acagaattta ataaaatcgc
     1861 gggagcggcc ggagcagcat tttccatatt agaaaaatac ttttacaatg aggaggaaat
     1921 ctaatgagtg ttttagtaac tggtggagca ggctatatcg gtagtcatgt agttaaagat
     1981 ttagttgaaa atgattttga tgttattaca gttgacaacc tacaaaaagg tcataaaaaa
     2041 gctgttttag gcggaaagtt gattgtggga gatattaaag atacagaatt cttacaagaa
     2101 gtatttagtt ataataaaat cgaggcagtt gttcatctgg cagcagatag cttagttggt
     2161 gaatcaatgg aagatccagg caagtattac cataataatt tggacaatgg aattaaactt
     2221 ttagatgtaa tgcttgaaaa cggagtaaaa aatatcgttt tttcttccac tgcggctgtc
     2281 tatggtgagc cggaagaagt cccaataaaa gaagatcatc ctatcgaccc cggtaaccct
     2341 tatggggaaa gtaaatactt ttttgaaaag gtaatggaaa ggtatgatca attatttgat
     2401 ctaaactata tttccttaag gtactttaac gcggcggggg ctgaccccag tggtcagatc
     2461 ggagaagacc atgacccgga aactcatctt atcccgctcg ttttagatac tgccctgggc
     2521 aaaagggata aactctatat ctttggtact gattatgaga ccagagatgg gacctgtgtg
     2581 agagactata ttcacgtcaa tgacctggct tctgcccata ctttagcctt acaggcctta
     2641 atgcaaggta aagaaagcag tatctttaac ctcggtaatg gtgaaggtta tacagtaaaa
     2701 gaagttattg aaactgcccg acaagtgacg ggagagataa ttcccgccga aaatagtgag
     2761 agaagagcag gtgatcccgc agtattaatt gcaagttcta aaaagattca aaaagaatta
     2821 aactggcagg ctgaatatcc agacttagaa acaataataa aaactgcctg gaactggcat
     2881 aagaaaggtg gctttaaaca atgaagatga aaaaagaaga agtcaaaaaa gatgatggta
     2941 ggttaatgta ttactataca tttgaagaag atgaggaaga taaatctaaa aagaaggagg
     3001 aaaacaaata aatgtctgaa atgagatgga atcccatatt aaaggaatgg ttaattacag
     3061 caactcaccg tcaggataga acctttaatc cacctgaaga ttactgtccc ttatgtccga
     3121 cagaaccggg tggatttgaa acagaagttc ccaggcctga ctatgacctg gttgtttttg
     3181 aaaataaatt tccttcttta gaaagagccc cacgtgaacc tgtagttgag ggtactgagt
     3241 taactccagt tacagattca aaaggtatat gtgaggtagt attatttacc tcagaccatg
     3301 agggagttat gtctgaaaaa cccttaagcc gctttatcaa acttatcaag gtctggaaag
     3361 atagatatga agatttaagt tccaaggaat ttatagatta tgtttatata tttgaaaata
     3421 aaggggaaga ggtgggggtt accctttcac acccgcatgg acagatttat gcttaccctg
     3481 atatccctcc tttaattgag aaagaattaa aaaatagtaa agaacatcta gaaaaagagg
     3541 gcagctgtct atactgtgat gttttaaaac aggagataga acaagaagaa agaatcatcg
     3601 ttcaaaatga ttcatttaca gcccttatac cattttttgc ccgttatgcc tatgaagtta
     3661 atatatatgc taatgaacat attacttctt ttgctgaatt taatgaaaaa ctaataaaag
     3721 atcttgccgg tgtcttaaaa accttgataa aaaagtatga taacctcttt ggttttgaat
     3781 ttccctatat aatggctatc catcaaaaac cggctgtaga aggaactgaa gattactctc
     3841 atttccatat tgaattttat cctccctata gaaccaagga taaattaaag tatttagccg
     3901 gcagtgaatc gggggcgggt gcctttataa atggttctct accagaagag aaggcggaag
     3961 aattaagaaa tacagaacct tttgaaatat gtgaagagta ggtggtaaaa atggaaaaaa
     4021 gtattttaaa agaacattat aaaaacaact tcaaaaatac aaaaaaaaga gatgggttct
     4081 tctttgcacc aggaagggtt aatctaattg gagaacatac cgattataat aatggttttg
     4141 tcttaccgat ggcaattgat agaaggatct atgcttatct tcaagtaaga gatgataaac
     4201 agatcaacat ctattccatt gatttagata gatattatac gacaactacc gaaaaactta
     4261 agttcaatga agaaaacagc tggtcaaact atattgtagg ggtagtcagc gaactacaaa
     4321 aagaaggcta tcacccgtcc ggcttcaaca tgaccttttc aggtgatatc ccccaggggg
     4381 ccggtttaag ttcttcggcc gctctggagg tagtaactgt gtatggatta gctgaattaa
     4441 acaactttga gatcagtcct gtcaagaaag ctttaatagc tcagagtgct gagaataatt
     4501 ttgtcggggt agaatgcggt ataatggacc agtacatttc ctgtttggga gagaaggata
     4561 aggcccttct aatagactgt gatagctatg attataataa ggtacctatt caaaataaga
     4621 agtatcagtt tttaataatt gattctaaag tacaaagggg tttggtggat tcagaatata
     4681 atttacgccg ccagcagtgt aataatgtgg ttaaatactt taaggaaaac tttgcagaag
     4741 atatagaaag cctgcgtgat attaatatta atgagctttt tagacatgaa gataaaataa
     4801 atgagaccga ttacaaaagg gccacccatg tcctgactga aaacagaagg gtattggagt
     4861 tcgctgatag tgtggaagat ggcaattatg aaaaggccgg tcagttaatg tatgcatccc
     4921 acctcagcct aaaaaatgaa tatgaagtta gctgtgagga gttggatttt ttaattgaga
     4981 ctgccttaga attaaaagga gtattgggag cccgcatgac aggggccgga tttgggggtt
     5041 gtaccgttaa tttgatcaaa aaagattata aaagtaagat tataaacagc attaagactg
     5101 aatataaaaa agaatacaaa aaggaacctg atttttacac cagccgcccg gcccaaggag
     5161 ctagttcata ttaaaatact ttagaaattt aataagaatt aaaagcagga ggttataatt
     5221 aaatatttaa tatacttaaa agaggaggga tgagtatgac tgaatataag tttccagaag
     5281 actttacttg gggggcagct gcagcttcct atcagataga aggtgcctat gacgaagatg
     5341 gcaaaggcga atctatctgg gataggttta cccatcaaaa aggaaatata aaaaatggtg
     5401 atacaggtga tgtagcctgt gatcattacc atagatataa agaagatgtt aagttaatga
     5461 aaaagattgg tcttgattct taccgttttt ctatagcgtg gccccgcatc tttccggaag
     5521 ggaccggttc aataaatcaa aaaggattag atttttataa aagacttgtc gatgaattac
     5581 tgcaaaatgg tattaaacca ctaccaaccc tctaccattg ggatttaccc caggccctac
     5641 aggagagagg tggttgggaa aacagagata ctgctaaata ctttcatgat tatgcgatgc
     5701 ggattataga agaatttgac ggcgtaattg ataagtggat cacgcataat gaaccctggg
     5761 tagttagttt tgtcggtcat gcatttggac agcatgcacc cggtaaacag gattatcaga
     5821 gtgccctgca ggtcctacac cacctgcttt tatcgcacgg tatggtagcc cgtaccttta
     5881 gagaagaaga aatgaatggt gagataggaa ttactgtaaa tctgagtgat tttcaacctg
     5941 ccactgataa gaaagaagat ttagcggccg cctggcgtca ggataacttc ctaaacaaga
     6001 tcgttttaga tcctttattc agggccaagt atccgaaaga actatttgaa ttcttcgagg
     6061 aaaatgtagg agaaattaag gttagagagg gagacatgtc agtcatcagt tatcctatgg
     6121 actttttagg tatcaactat tattcccgca atgttataga acatgcagaa gaagccaata
     6181 tattagaaag caaggaagta aaaatagagg acagccaata tacagaaatg ggctgggaag
     6241 tatatcccca gggcttatac aatgtcttaa acagggtaaa taatgaatac tctgatttac
     6301 ccctatatat cacagaaaat ggggctgcct ttaaggataa attaactgaa gatggtaagg
     6361 taaaagatga taaacgggtt aattacctaa aaaaccactt attaaaagcc cagcaggcta
     6421 ttgaagacgg tataccactc caaggatact atgtttggtc tattatggat aactttgaat
     6481 ggggctatgg tttctctaaa agatttggta tcacctttgt ggattacaag gataaccaaa
     6541 agagatatat caaaaaaagc ggtaaatttt acagtgaagt aattgaaaga aatggcatcg
     6601 agatcaaaga ttaatctttg gtctcttttt ttgtttttaa taaaaagaat tttggaaatt
     6661 gtttgacaat taaaaattct tattatataa ttatcaatgg ttaaaacgtt ttaaactaca
     6721 taataaagaa ggttatcagc tatgtctaca accttaaaag atattgctaa aaaagccaat
     6781 gtttcagttg caacggcttc tttggcttta aataatagtg aaaaagttaa tcacaaaacc
     6841 tacgaaaaga tcaaaaaaat cgcggaagaa ttaaattaca ccccaaatgc gagggcccgt
     6901 gctctcgtta aaaaagcgac cgaaaccatc ggcctcgtca tcccggaagt tgaaaacccc
     6961 tttttcgctg aaatggccca gtctattaaa aatatgatta aagaaagtgg atataattta
     7021 atattatgta gtacagaata taagtcagaa gaagaattaa gatacatcaa tctctttaaa
     7081 agcggtctgg tggacggagc tatttttgcc tgtttgggcg atatgatgga agaacataat
     7141 aagatgatca ttgatctcgt taaaaataac acgccagttg tatttgtaga ccgtgaaatt
     7201 aataattata atctaatccc agtgatcaaa accgacttat ttgaaggtgc ctataatatc
     7261 gcaacttatc taattaacaa cggccataaa aagataggtt ttgtcggtca gagtgaagag
     7321 agggtaaaag gctataaaaa ggctttaaaa gataataata ttaagataaa tcaagatttc
     7381 atttattacg actttttaac catcgaaggt ggttataagg tcggtcaaaa aatagtaact
     7441 ttagatgaac ttcccaccgc cttgatctgt ttgaatgatg aaatggcaat gggggttatt
     7501 caatccttaa cttctgctga tatagacgta ccagatgata tatcaattat tggtatagat
     7561 aacattcgga tgtctgagta ctataatcct tccttatcta ctttacatat acccaaaaaa
     7621 gaaatgggta aaaaagcggc ggaagtactt cttaaaagaa taaaaaaaga aaggttaagt
     7681 tcagacgaac agcttataat ctttccaacc gagttaatag ttagaaaatc ttctaaaaga
     7741 ctcaactaaa tttgcatgga ggtggtatat tttattgagc agctttaaaa cgttttaaaa
     7801 ataattttta aaaaacttag gggggaatat taaaaaatga aaattaagaa gttattacca
     7861 gttctattag tcctaacttt attatttgca tttagtgcag ttacgatggc agagacactg
     7921 accgttaata catatacaag tgatcctgcc ccaagaaagg ctatgaaaag gttagtagag
     7981 atctatgaag agaataatcc tgatattgat gttcagctca atattactgc tcatgaggac
     8041 tttaaaaagg cgattagagt ttggctatca tctgataatc caccagatgt attcagttgg
     8101 tttgccggaa accgggcgaa gtactttgtt gataaagact tagtaatgcc ctttactgat
     8161 gtctggcagg aagagaacct tgatgaaaag ataccaaggg cctttaaaag tataagtttc
     8221 tttgaagatg aagcctactt cttaccacat agttactact ggtgggcaat ctactataga
     8281 gaatcaatct ttgagaaata tgacctcgaa gaaccacaaa cctgggaaca actacttgaa
     8341 gtaggagaaa ccttaaaaga aaacggcatt actccttttg ccatcggtac caaatttaaa
     8401 tggactgcag ccggttggtt tgactatcta aatatgagga tcaatggacc tgaattccac
     8461 gttgatttaa tgcacggaga agtaccattt accgatgatc gggtggcaga agtatttaaa
     8521 aaatggaagt acttaattga aaatgacttc tttgttgaag atgcagctgc ttactcctgg
     8581 caggaaggtc tccagtttat gacccagggc gatgctgcta tgtatctgat gggtcagttt
     8641 atcaatgacg cggtttctga agacgtgcaa aaagacatga acttctttag attcccaatc
     8701 attaataaaa atgtacctat tggcgaagac ctacctacag acggctttat gattcctgct
     8761 aaggctccta atcctgaact agccaaagac tttattgctt tcttagctag tgtagaagga
     8821 cagagagtat ttgcggaaga tatgggacgt atggtgccta atagtgatgt acccgatgaa
     8881 gtatatgatg aaccagctct taaagggaaa gaaatgattg aaaacaccga tgccttagct
     8941 cagttctatg atagagacat ggatcctgcc atggcaggag atactatgga tttactcatg
     9001 aagttctggc agtatcccga tcagtacgaa tcaattatgg aagagatgga acaggtaaga
     9061 cagaaggtta tggaataaaa ttatataaag aaagcagggg ttaattcccc tgctttctca
     9121 ttaaattgtt acagtggagg ttgcagtcaa aatgaaaaaa agaaaagtac agtggcgttt
     9181 tttagccatt ccacttattt tttatacaat ttgggtaatc ggtcccacta tctatacatt
     9241 ctatcttagt ctgacagaat gggacggtct aactgacccc agctttattg gcataactaa
     9301 ttatttggat ttatttaatg attctgtatt ttatacttct ttagttaata attttatttg
     9361 gctttttatc tttttattaa taccggtagt tttaggccta tttttagcaa ttttacttag
     9421 caagaaaatc agatgggcga aaggctttaa agcagccatc tatagtccta tgattctggc
     9481 tccagttgtt atcggtttaa tctggtcctg gatttaccat cccaaggccg gaatactgaa
     9541 caatacttta acctatgtcg gccttgattt tttgacaaga ggttggttga gtgacccgga
     9601 aacagccctt tatgcggtaa tcggagcggc tgtttggcgg catacgggtt atgtaatgct
     9661 tttattttta gcgggtttaa cctcaattcc acctacttta attgaagcag ctaggatcga
     9721 tggggcaaca gcctggcaga gatttagata tataatccta cctctcttaa agccggccac
     9781 tgttatcgtg ttggtaatta ccatgattca atccctaaga gcatttgact ttgtaaatat
     9841 catgacccgc ggtggcccct ttaattcgac caatgtttta gccaactata tgtatacgga
     9901 agccttccgt aactatgaga tgggctacgg ggcagccata gcggttatct tatttatcat
     9961 catgtttgtc tttattttct tctacttaag aagaatcaga gaggaggagc taaatgtatg
    10021 aactacctaa agaaaagaaa atatcttaat gcatttatct ttatagccct cctccttttt
    10081 gttatcgcct ggctgttacc ggtctttggt gcatttttaa ccgcttttcg gtcaatggat
    10141 gaaataatgg acaagggatt ttggacaatg cccgaaaaat tatcctttga taattttaaa
    10201 gtagcctgga gtgaaggtaa tttaaataca tatcttttaa acagtttcat aatcacaata
    10261 ccttctttgt taggaactct atttttatct tcattagcag cctatgcttt agcacagtat
    10321 gattttaaat tgaacatgcc cctttattta atgtttgtgg gcggtatgtt aattcctttt
    10381 caaatccttt taattccggt ctttaagtta agtaactttt tgggtattta taatacctat
    10441 tggggattaa taatgattca tatggtattt caaataggtt tttgtacctt ctttttaagg
    10501 aactttatga tgacgatccc ggaagaacta tcagaagcag cccgtatgga cggttgttcg
    10561 gagtttaaaa tattttaccg aatctattta ccactttcta aaccggccct ggccgctttg
    10621 ggaacattag aattcacctg gatctttaat gactatatct gggcattagt attaattagg
    10681 gacaataaat ttaaacccgt tacggccggt ttggcttctt tgaaaggtaa ctttataaca
    10741 tcctggggag ttttagtagc gggttcctta ttagctacaa taccaactat tattgtcttt
    10801 atcttcttac agaagtactt tattagaggt ttaacaatgg gtacaggtaa atagcgaaca
    10861 aatggaggtt tttaatatga ggcagatctt aaattttaat acagaatggc agtacttacc
    10921 tgaagataat aaaaagatga aagatattag ttacagtaat tccaaaatag aagatatcag
    10981 tattcctcat tccaataaat tggttcctca ccactatttt aatcatacag aatatgaatt
    11041 tatttcctgg taccggaggc cgttttatct ggatgaaagt tatgaagaca aaaagatctt
    11101 agttaagttc gatgggataa tgaaaaaggc ggaagttttt gttaatggaa ctttagtcgg
    11161 tacacataaa ggtgggtata cttcctttaa attagatatt actgattatg ttaatattaa
    11221 cgagaataa
//
LOCUS       DATGCJ010000055         6088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_587023, whole genome shotgun sequence.
ACCESSION   DATGCJ010000055 DATGCJ010000000
VERSION     DATGCJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587023"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VKN64_02710"
     CDS             complement(<1..1525)
                     /locus_tag="VKN64_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59177.1"
                     /translation="MSKKLFSIIVTAALLISIFSLSAAAQSDDQTLVIGFEADVSSLD
                     PGFADISVNERVNSLIYDGLVEYGENNKIVPGIAYDWDISEDGTIYTFYLQQGIPFHN
                     GESLTAEDVKFTYDRILDPESGSGLRGKVTIIDRIEIVDDYTVRFVLKEPYSPFMLAM
                     TFGIVPKDYVERVGADEFSRNPVGAGPFKLEEWVPDTRIVLTANETYWMKDPNLSRVI
                     IRPIPESSVQAMELRSGGIDVAVSLDVGQLDNFKDEADFQVKSAAGGGVHFFGFNDKM
                     DPFNNVEFREASLMASPIEQIVPQIYGPLGVTAYSFVPPTLWPDEKDYLKEQRINFNP
                     QMAQERVQELKDKGIIDDDFTIDIYVPSSNNSRIKVVEALVTSLRQAGFKAQAQVMEF
                     GTMWDKLGAGEIGVYMLSFVSDPDPDYWLYRWFKSDGSLNKSFYENDQVDEWLETART
                     SSDQGVRAEMYSKVLRKTLGDDKVLVPVAHINQIYVMNNSVKELAPGNPILIPLVTPD
                     "
     gene            complement(1546..2313)
                     /locus_tag="VKN64_02715"
     CDS             complement(1546..2313)
                     /locus_tag="VKN64_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HMA59178.1"
                     /translation="MRPRILVSSFLREGYTFGPNFKMPSSYDYGMVSQNYVNGVLEGG
                     GVPLVSPQIRKHYDNDQLMEDLVDSVDGIVLTGGYDVDPAHYKKHAYKKVKFVDPIRD
                     LQELKLVELALKKGKSILGICRGFQVLNVFFGGNLYQDINQDLETEVCHFGSGLPKYT
                     KVHSVSLEKDSILEQIFEDNPIEVNSFHHQAVNKVGKGLRETAFSSDGILEGFESTEY
                     ENVWAVQWHPESMFSNHPEQLAIFREFIKRTRKSNTK"
     gene            2548..3402
                     /gene="folD"
                     /locus_tag="VKN64_02720"
     CDS             2548..3402
                     /gene="folD"
                     /locus_tag="VKN64_02720"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HMA59179.1"
                     /translation="MSKIIDGKQIAKNIRMELKDEIKDLKEKGRVPGLTVVMVGNNPA
                     SETYVSMKEKAATEIGINSTLIKKEEDISEKELLDLIDDLNNDSAVDGILVQLPLPDH
                     IDEKKVIESIKPDKDVDGFHPINTGRLFSGQEDSLRYEACTPLGIIRMLEREGIEIDG
                     KNATIVGRSNIVGKPIAHLLLERHATVTICHSHTKDLKAETKRADILVAAVGRPEMVT
                     GDMIKEGAVVIDVGINRVEGELLGDVEFETAKEVASKITPVPGGVGPMTIAMLMENTV
                     KARKYHGV"
     gene            3392..4621
                     /gene="xseA"
                     /locus_tag="VKN64_02725"
     CDS             3392..4621
                     /gene="xseA"
                     /locus_tag="VKN64_02725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMA59180.1"
                     /translation="MEYNIDNIYSVSQISNYLEALFSTDEVLQDLWVKGEVSNFYQAN
                     SGHMYFTIKDEDAQLKTVMFRYKSKGIDFELEDGMEIAVHGYINIYKVRGEYQLYADE
                     IIPEGKGSLYEAFEKLKKKLKEEGLFAEEHKKKIPMIPKKIGIVTSPTGAAIRDMLSV
                     VDRRFEHVSILIVPSLVQGDKAADQIVAGINYLNSRDDIDLIIVSRGGGSIEDLWPFN
                     EEKVARAIFSSEIPVISGVGHETDFTIADFVADLRAPTPSAAAELAIGNREEIENHID
                     NLARRLLHNQKARLKNARERLSSISNRRIFKKPEELFINKMQKLDDLSRELKWNMEKK
                     LNKSKERFKILSSKMDTLSPLKTIKRGYSITYDEDENPVTDISEVNKGDKVKTTIING
                     SFLSTVDQIEEDGGINE"
     gene            4614..4856
                     /gene="xseB"
                     /locus_tag="VKN64_02730"
     CDS             4614..4856
                     /gene="xseB"
                     /locus_tag="VKN64_02730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMA59181.1"
                     /translation="MSEKKDLNFEKAMEELEEIVEDLEEGGLPLEESLDKFSRGIELI
                     KYCNEKLGETEKKIEVLIKEDEEIKDIVPFEEEFGQ"
     gene            4884..5333
                     /gene="nuoE"
                     /locus_tag="VKN64_02735"
     CDS             4884..5333
                     /gene="nuoE"
                     /locus_tag="VKN64_02735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMA59182.1"
                     /translation="MEVIKSLEKDQSNLIKAIQMVQRERGYISDQAIKDISEYFDIPT
                     VEVEGVLSFYAQFKRTAPGEYQIAVCDGTACHIKGSSRVKGWLEEELQIEDGETTDDG
                     LFSMETVACLGCCSLAPVVSINGKVYGKLDRKKLLKIIDDYRNRGEN"
     gene            5334..>6088
                     /locus_tag="VKN64_02740"
     CDS             5334..>6088
                     /locus_tag="VKN64_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HMA59183.1"
                     /translation="MIERIKVGMASCGIAAGADEVKDFLDEKLKDKDIEVTEVGCIGH
                     CYAEPLVEVETDSGSVFFENVEPTEEDLNKMLELDEESTMSFRKERTGREHLKVTKLA
                     GRIDPVSLDEYIEHGGYESLKKAADMKPEEIVEEVKTSGLRGRGGGGFPTGLKWSFLA
                     GQEADKKILICNADEGDPGAFMDRSMMESVPHQVLEGMLIASLATGASELFIYARAEY
                     PLAVRNMNKAIDQVYENELNVFDGKTIDITVKEG"
BASE COUNT         2011 a         1116 c         1284 g         1677 t
ORIGIN      
        1 catcgggggt aacaagtgga attaaaattg gattacccgg agctaattct tttacactgt
       61 tgttcatcac atagatttga ttgatatgag ctacaggtac caataccttg tcatcaccca
      121 gggtttttct taatacctta ctgtacatct cagcccttac accctgatca gaactggttc
      181 tagcggtttc taaccattca tcgacctggt cattttcata gaatgattta ttgagtgaac
      241 cgtccgattt aaaccaccta tataaccagt aatcggggtc tgggtcagaa acgaagctta
      301 acatgtatac accaatttct cccgcaccta acttatccca catggtgccg aattccataa
      361 cctgggcctg tgctttaaag ccggcctgtc tgagggaagt aactaaggct tctacaacct
      421 ttatacggga attattggaa cttggtacat agatatcaat tgtaaagtca tcatcgatga
      481 tacctttatc ttttagttcc tgtacccttt cttgtgccat ttgcggatta aaattgattc
      541 tttgttcttt taaatagtct ttttcatccg gccagagggt tggaggcaca aaggaataag
      601 cggtaactcc taaagggcca tagatttgtg gtacaatctg ttcgatcggg ctggccatca
      661 aagaggcctc tctgaactca acattattaa agggatccat cttatcatta aatccaaaga
      721 agtgaacacc accaccggct gcagatttaa cttggaaatc agcttcgtct ttgaagttat
      781 caagctgacc tacatctaaa cttactgcaa catcaattcc accacttctt agttccatgg
      841 cctgaacgga agattcaggg ataggtctaa tgataaccct acttaaattg ggatctttca
      901 tccagtaggt ttcattggct gttagaacta ttctggtgtc aggtacccat tcttctaatt
      961 taaaagggcc tgcccctacc gggttacgtg aaaattcatc tgccccgact ctttcaacat
     1021 aatctttcgg aacaatacca aaggtcatgg ccagcataaa gggggaataa ggttctttta
     1081 acacgaatct aacggtataa tcatcaacga tttcaatccg gtcgatgatg gtaaccttac
     1141 ctcttaagcc agagccactt tccggatcta agattctatc ataggtaaac ttaacatctt
     1201 ctgcagttaa ggactcaccg ttatggaagg gtataccctg ctgtaaatag aatgtataga
     1261 tcgttccatc ttcagagata tcccaatcat aagcaatacc aggtacaatc ttattattct
     1321 caccatattc aacaagacca tcataaatca agctgttaac tctttcatta accgaaatat
     1381 cagcaaagcc cgggtcaaga ctgctcacat ctgcttcaaa accaatcacc agggtttggt
     1441 catcggactg agcagcagcg ctcaagctaa atatactgat aagtagtgcg gctgttacta
     1501 taatagaaaa tagtttttta ctcatcattc tctctcctcc ttatattatt ttgtatttga
     1561 tttccgagtt cttttaataa attctcggaa gattgctaac tgttcggggt gatttgaaaa
     1621 catactttcg ggatgccact gcacggccca gacattttca tattctgtgc tttcaaatcc
     1681 ttctaaaatt ccatcagaac taaaggctgt ttctctcagt cctttaccta ctttattgac
     1741 tgcctgatgg tggaaactat ttacttcaat cggattatct tcaaagattt gctctaagat
     1801 actgtctttt tcaagagata cgctgtgtac tttagtgtat ttaggtaacc cactcccaaa
     1861 atgacatacc tcggtttcta aatcctgatt gatgtcctga tagaggttac caccaaaaaa
     1921 tacgttgagc acttgaaaac cccgacagat acctaaaata gatttccctt tttttaaggc
     1981 aagttcaacc aattttagtt cctgtagatc gcggatcgga tcaacgaact tcaccttttt
     2041 ataggcatgt tttttataat gagcaggatc tacatcataa cccccggtca agacaatgcc
     2101 atcgacagaa tcaacaagat cttccattaa ttggtcattg tcataatgct ttctaatctg
     2161 gggtgaaacc aggggaactc cccctccttc taatacaccg ttgacataat tttgtgatac
     2221 cataccataa tcatatgaac taggcatttt aaagttagga ccaaatgtat agccttctcg
     2281 taaaaaagaa gataccagta ttcgcggtcg catattattc ctccatcctt ttatattagt
     2341 aattaataac agttgacagt atttgatata tttaatatta taaaaaaggt gattaaaata
     2401 ctaataaaaa tattatatca gtaaaaccca ctgcaagcaa caaattaact atcatatttt
     2461 ctcaatataa tagatattta aggaggaaat ttataaatat tcaagaatag ataaatagat
     2521 aaatatctaa aaaatagggg gataaaaatg tctaaaatta ttgatggtaa acaaatagca
     2581 aagaacatta gaatggaatt gaaggacgaa ataaaggatt taaaagaaaa aggccgtgtg
     2641 ccaggactta ccgtagttat ggtaggaaat aatcctgctt ctgaaactta tgttagtatg
     2701 aaggaaaaag cagccacaga aattggtatt aattctaccc ttatcaaaaa ggaagaagat
     2761 atcagtgaaa aagaactgct cgacctaatt gatgatctaa acaatgattc agcggttgat
     2821 ggtatcttgg ttcaactacc tttacctgat catattgatg agaagaaggt aattgaatcg
     2881 atcaaacccg ataaagatgt ggatggtttt catcccatta ataccggtag actgttcagc
     2941 ggtcaggaag attcattaag atacgaagcc tgtacaccac tgggcattat cagaatgctt
     3001 gaaagagaag gcattgaaat tgacggaaaa aatgctacta tcgtaggaag aagtaatata
     3061 gttggtaaac ctatcgctca ccttctatta gagagacatg cgacagttac aatctgtcat
     3121 tcacatacaa aagatctaaa ggcagaaaca aaaagggctg acatcctggt agcagctgta
     3181 ggaagaccag agatggttac tggcgacatg atcaaagaag gcgctgttgt catcgatgtt
     3241 ggtatcaata gggtagaagg agaactgctc ggcgatgttg aattcgaaac tgccaaagaa
     3301 gttgcttcta agattactcc tgtacccggt ggagtaggcc cgatgaccat agctatgctc
     3361 atggaaaata ctgtcaaggc cagaaagtac catggagtat aatatagata acatatattc
     3421 ggtctcccag ataagcaact atctggaagc attattcagt accgatgaag tactacagga
     3481 cctctgggtt aaaggagagg tttctaactt ttatcaggcc aattccggcc atatgtactt
     3541 tactatcaag gatgaagatg ctcaactaaa gaccgtgatg tttagatata aaagcaaagg
     3601 tatagatttt gaattagaag acggtatgga gatagccgta cacggctata tcaacatata
     3661 taaggtaaga ggtgaatacc agctttacgc tgatgaaata atcccagagg gtaagggatc
     3721 cttgtacgaa gcctttgaaa aactaaaaaa gaaattaaaa gaagagggac tatttgcaga
     3781 agaacataaa aagaagatcc cgatgatccc caaaaagata ggtatcgtta cttcaccgac
     3841 cggggcggct atcagggata tgctctctgt ggttgataga aggttcgaac atgtttcgat
     3901 tttaatcgtg ccctctctcg tccagggaga caaggcggcc gaccagattg tggctggcat
     3961 caattacctt aacagccgtg atgatatcga cttaataata gtcagtagag gcggaggttc
     4021 cattgaggac ctctggcctt ttaatgaaga gaaggtagct agggctatct tttcttcaga
     4081 gataccggta attagtggag tcggtcatga gaccgacttt accattgccg attttgtggc
     4141 tgacctgagg gcaccaaccc cgtcagctgc agctgaactg gcgatcggta accgggaaga
     4201 gatcgaaaac catattgata atctggctcg gcgtctgctg cacaaccaga aagcccggct
     4261 taaaaatgcc agagagagat taagttcaat ctctaaccgc cggatattta aaaaaccgga
     4321 ggaactattt atcaacaaaa tgcagaagct ggatgattta agccgtgagt tgaagtggaa
     4381 tatggaaaag aaattaaata agagcaaaga aagatttaag atattaagca gcaagatgga
     4441 tactttatct cctttaaaaa ccataaaaag gggttacagt attacttatg acgaggatga
     4501 gaatcccgtc acagatattt ctgaggttaa taaaggtgat aaggtaaaaa caactattat
     4561 aaacgggagc tttttaagca ccgttgacca aatagaagaa gatggtggta ttaatgagtg
     4621 agaaaaaaga tttaaacttt gaaaaagcga tggaagagtt agaagagatc gttgaggact
     4681 tagaagaagg gggcttacct ttagaagagt ccttggataa gtttagccgc ggtattgagt
     4741 taatcaaata ctgtaatgaa aagttaggtg aaacagaaaa gaaaatagaa gtactgatta
     4801 aagaagatga agaaataaaa gatatagttc cttttgaaga ggaatttggg cagtaataaa
     4861 actagagaat ggaggctcaa aaaatggaag taattaaatc tctggaaaaa gaccagagca
     4921 acttgataaa agcaattcaa atggtccaaa gagaaagagg ctatatttca gatcaggcca
     4981 taaaagatat ctcagaatac tttgatattc cgaccgtgga agtagaagga gttttatcct
     5041 tttatgccca gtttaaaagg accgccccgg gtgaatatca aatcgctgtt tgtgacggga
     5101 cagcctgtca tattaaaggc tcttcccggg ttaaagggtg gttagaagaa gaactacaga
     5161 ttgaagatgg tgaaactaca gatgatggac ttttttccat ggaaacggta gcctgcttag
     5221 gctgctgtag tttggcccct gtggttagca taaatggtaa agtatatggt aaacttgacc
     5281 gcaaaaaatt acttaagata atcgatgatt acaggaatcg aggtgaaaat taaatgatag
     5341 aacgcatcaa agtagggatg gccagctgcg ggatagcagc cggtgccgat gaagtcaaag
     5401 acttcctgga tgaaaagtta aaagataagg atatagaagt aactgaagtg ggctgtatcg
     5461 gacactgtta tgctgaacca cttgttgagg ttgagacaga cagcggatcc gtattttttg
     5521 aaaatgtaga accgactgaa gaagacctca ataagatgtt agagcttgat gaagagtcca
     5581 caatgtcttt tagaaaagaa aggacgggcc gcgaacacct taaagttacc aaattagccg
     5641 gtcgtatcga tccggtttcc ctggatgaat acatagaaca cggcggttat gagagtttaa
     5701 aaaaagcagc tgatatgaaa ccagaagaaa tagtagaaga agttaaaact tccggtctga
     5761 gaggacgcgg aggtggaggt tttccgaccg gacttaaatg gagtttttta gccggtcaag
     5821 aagccgataa aaagatctta atctgtaatg ctgatgaagg tgacccgggt gcattcatgg
     5881 accgttccat gatggaatca gtaccccatc aggttctaga aggtatgtta attgcatcgc
     5941 tggcgaccgg ggcatctgaa cttttcattt atgcaagggc cgaatatcct ttggctgtaa
     6001 gaaatatgaa taaagcgatt gatcaggttt atgaaaatga actgaatgta tttgatggaa
     6061 aaactattga tataaccgtt aaggaagg
//
LOCUS       DATGCJ010000056        15580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_453681, whole genome shotgun sequence.
ACCESSION   DATGCJ010000056 DATGCJ010000000
VERSION     DATGCJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453681"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VKN64_02745"
     CDS             <1..470
                     /locus_tag="VKN64_02745"
                     /inference="COORDINATES: protein motif:HMM:NF037860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HMA59184.1"
                     /translation="VGEAGIKVNEIACRIGGAYEADFMPLITDVDILKMVINSSLGKS
                     INKEPLKNYNLLENDNWLSVQLFFAGKGKIKSIRNIEHIKNIPEVVDIKVNYKEGDTI
                     PEIENATARAGYFIVKAKSKRELKNNIKKVYDKLKILNTKDKNLVLRKAGETL"
     gene            complement(618..2162)
                     /locus_tag="VKN64_02750"
     CDS             complement(618..2162)
                     /locus_tag="VKN64_02750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMA59185.1"
                     /translation="MIKLKNLNKLYVILIIYFLANLLFLTSFPFVHSDEAWLAGLSRT
                     ILKEGTYDVTEPFFDLYPRSPHAIRVIFHSIQVGFLKIFGYEIFTFRLISLIFGTISL
                     FFFYRITFILTKSKKLSYLTTILLAVDIQFIYTSHLARQEIILVSIFLMALYYFLTKV
                     IQDKYYSSNFLLGLIISISIGIHPNSFITFIPFLFLYLYYIIFTEKLNYMDLLVFLTT
                     VFIGALIFILMSLYFDPNFIYNYKSYGETLGVFKSLQIKLDRLDYFYKKLYYQVSGTY
                     FTPDIKPQFYLFGLTIVYSIYKLFTKKDNFINILLLSFIGINLGYIMIGRYNQISIIF
                     IFPILYFLLANLIKNIDPDYSKTILTALIFILLLNSFIIIYNNSYFNYKDYLNDISTT
                     INKEEKVLANLNTQFYFDYNKLYDYRNLAYLDENNLSFSEYIEKNNIKYIIYPEEMDF
                     IYNRRPTWNTLYGNLYPYYSDMKDYLVTDCRLAKEFSNKTYGMRIAKYIGTKDWKVKI
                     YKVIDN"
     gene            2479..3381
                     /locus_tag="VKN64_02755"
     CDS             2479..3381
                     /locus_tag="VKN64_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMA59186.1"
                     /translation="MRRNKLLSVLFIVALTLVVMSSSVLAVSDAVEDAVNGYFANLPG
                     DNAMIGQEAFVDKLKAGEDLVVLDIRQPDVYNEGHVKGAVNIPWGPDAMPAALDKLPG
                     DETIYVYCYTGQTANQAVGLLNFAGFEAKSVKFGWNLGIAKVDGYEEVVETTANELGG
                     ETDYKINEDIKTAVVDYYKGLADVSDTMFKNYKISEDMLKMALDSDSDLMVVSIRQPS
                     AYSEGHIEGAINIAWGKGMQEYFGQLPQDKKLVVYCYTGQTAGQAVAGLRMLGYDAVS
                     LNGGMGTPANEPYGWSNKGYETVQ"
     gene            complement(3460..3897)
                     /locus_tag="VKN64_02760"
     CDS             complement(3460..3897)
                     /locus_tag="VKN64_02760"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HMA59187.1"
                     /translation="MRIIIDGDACPVIDLAESIAHKFNIELILFCDYNHDIKLNYGRV
                     VKVDQSYQSVDMKIINFAKRKDIIITDDYGLASLALSKKASAINSKGKIYTDKNIDTL
                     LMKRHINKKIRRAGGRHPTAKKRSKKDDEKFKINLIQLIKDIK"
     gene            4230..5120
                     /locus_tag="VKN64_02765"
     CDS             4230..5120
                     /locus_tag="VKN64_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HMA59188.1"
                     /translation="MYIKSLKYFQEIAKIKSISKVANNSHISQPALSQQIQKLEDFLG
                     TELFIRSNRGVELTESGKIVLKYADNIIRTYDKMLSELDEQDSKEIKIEGEHTIATYC
                     LPCVILNMQMKFPKHRYNLVSASPDKIEQDVASDICEIGFTTTPVRDESLNSKNVIDE
                     KVVLISPPSYDMPEKINLTDVLDNKFIILKEDCIIKENFKDGLKEKGHSFNDVEVISR
                     LDSTEAMKTLVRKGYGVAFVPYNSVRDEFSANEIRVSRITDYNLDYDIYMIYKKAEFL
                     SKEAKEFIRHFKQLGKHVCY"
     gene            5268..6188
                     /locus_tag="VKN64_02770"
     CDS             5268..6188
                     /locus_tag="VKN64_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMA59189.1"
                     /translation="MKKIAKIIFVLLTFAIIFTGCEADITFPGEVGQDIITANEALKM
                     IENEDVVVVDAQKSGDYEDQHLEGAVNIARNDITTFGPFPNMLASASKIEETLGENGI
                     SNNSKVLIYDNNNNMDAARLWWTMLVFGHDTANMKVISGGLNALQDEGASFASGETEV
                     ETVEYNASEKNEEYVATKEEVLAEVNEPSEDTVILDVRTTEEVNEGIIPGAVHINYID
                     NCKDNGEYYPPSYIQRYYPDNEVKPNQKVIMYCKTSIRAAQTFLALHNAGYRNLEIYD
                     GAWIEWSSDSSLPQGMPSGEPVEPSQQDAS"
     gene            complement(6722..7519)
                     /locus_tag="VKN64_02775"
     CDS             complement(6722..7519)
                     /locus_tag="VKN64_02775"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59190.1"
                     /translation="MTVETEKETLIKIENMSVLYGKEEKSTLALKDINLEIKRGEFVC
                     VLGPSGCGKSTLLKTIAGYLKPSSGRCLMQGKEITGPDWHRGVIFQSPTLYPWMSVKD
                     NVEFGPKMRDLPKEEIKKIREYFLDEVNLSGVGDQYTFELSGGMKQRVALARVLANYP
                     QVILMDEPFGALDALTRDNMQNLIRDIWVENNTTIFFITHDVDEALSLATRVVVLSKL
                     PGEIIKIFDVDFTKRIFEEGTKDVIYKDNYFEVKNEILDIIHSQIEE"
     gene            complement(7552..8613)
                     /locus_tag="VKN64_02780"
     CDS             complement(7552..8613)
                     /locus_tag="VKN64_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA59191.1"
                     /translation="MKNSFIKIMTVVLLIGLLVFSLSGCGNNQSGQDGNQEVLPEEIN
                     FGILRIPNDETIAIAKGYFDKYFADRGIEVNTTVFDSGAEANQALASGSIDFATMGNT
                     NAITALSRELKVEMVWLHEVLGDNEALAVKNGSGIEKIEDLKGLRVAVPFASTAHYIL
                     LNVLKEAGIEDDVQLLDMQTAEIVAAWERGDIKAAYSWQPTLAKLLDNGRALITSEDM
                     AEKGYITANVEVVRKEFSNKYPHLVADFISAMAEAGDIYRENPQEAAEIVSGELGIEA
                     EVALHQMRGTSWKTREELLSEDFFGTVENPGNFAKVMKDTSDFLQNQGSIEVSPSQET
                     FNEYVNPEYIKMSLDNEQE"
     gene            complement(8649..9512)
                     /locus_tag="VKN64_02785"
     CDS             complement(8649..9512)
                     /locus_tag="VKN64_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59192.1"
                     /translation="MALKNNEQPKEENKNKTKWADKLINRRVDEDKKTKTEKILTFTT
                     WASILILWYVITKLEIFSPTLLPGPFRVWQAFLHILENGYNKVPIWVHLGASLKRLFV
                     ALFFAIFTAVPLGLLSGYFSKVEAIIDSVIEFYRPLPPLAYYTLLVLWFGIDDLSKQV
                     LLFLAAFAPIYIACVSAVRKLNRDFVLSAESLGAEQKDIFFKIVLPASMPEIFTGIRT
                     AFGVAYTTLVSAEMVAATSGIGWMVLDASNFLKSEVIFVGIIIMGITGVIIDAGLRYL
                     EENIIFWKGHL"
     gene            complement(9705..11402)
                     /locus_tag="VKN64_02790"
     CDS             complement(9705..11402)
                     /locus_tag="VKN64_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA59193.1"
                     /translation="MSKKILVVGGVAGGASAATRARRLDESAEIVMFERGPHVSFSNC
                     ALPYHISGVVKECDDLVLMCPENFAEQYNIDARVRNEVTKINRNEKTVEVKDLESGEV
                     YTEDYDKLILSPGANPIRPQSIKGVNNDNVFTIRNVVDIDKLNQYINKNNIENVAVIG
                     AGYIGIEIAENFIHSNKNVSLIEALDQVLQPLDYDMAQIVHKELHDNGVNVVLSDPLS
                     EVHDDHVVLKSGKKIDAGAVVLSIGVNPETQLAEDAGLEIGKTGGIKVNHNYITSDPD
                     IYAVGDVIEEYCRISRSKTRMPLAGPAQRQARAAANHIYNIPHKNNGVIGSSVIRAFN
                     YNAATTGLNEKQLQDAGISYDFVYIIPQDKVGLMPNSNPLHFKLLYETPTGKVLGAQA
                     VGKGNVDKRVDIIATAIMMDATVEDLIGLELCYAPVFSTPRNAVNQSGMVANNILNNR
                     YNQVPVTKVRELVENDAFIIDVREEDEYEEGHLVNAVNIPLSELRGRTDEIPEDEPVY
                     LHCRSAQRSYNAVLALQGMGYDNVVNISGSFLGICCYEYFLDQTTDRDKIVTEYNFE"
     gene            complement(11462..12727)
                     /locus_tag="VKN64_02795"
     CDS             complement(11462..12727)
                     /locus_tag="VKN64_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HMA59194.1"
                     /translation="MKKKSEKFIGFIAILVILIAAKFWLKTDILFFRLLIGTGIGYAL
                     ARAYTGFAGSVNRAYRTGSTKLMRTLMFMFFITAVLTAGLLLGNDPASFNLWVKPINL
                     GVIFGGFLFGFGMSLSVCCASGVLTDLPMGFPRAFTTLIFFGLGVFLGFPVQNTASWV
                     QESWFSSYIGYRTMGGVFLPDFFQGDGFEGYLGGLIALGALCVIFTALSYLYERYQVK
                     NNKYTGLEIERIQENAESIDFKNFKLFSADTYYHFFEKPWTLKQGAVALAIIFTLLMA
                     VTRAGWGASQPYGIWFGKFLMIFGISAESLSSFTRMSAEVFNTPFFEHQISVQNFGII
                     TGAIIYLLTAGKFSKMFTSELSITKKQAAIYALGGLTMGFGTRLANGCNVGALYTPIA
                     NFSLSGWLFLVFMIVGGIISNKFNEKIAG"
     gene            complement(13262..13903)
                     /locus_tag="VKN64_02800"
     CDS             complement(13262..13903)
                     /locus_tag="VKN64_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMA59195.1"
                     /translation="MKYKFFIIITILILIISFNITILAQEDIQEVEINEYEGEKLGSI
                     EDFRENSIKGIQEVDIEEYRLIVDGLVDNKQEYKYSVLQEKEHIKKVITLNCVTGWSV
                     KALWEGIPLKSIFKEVEVKDEANTVIFYSPDGYTTSLPLSFIMENNIMIADKVNDVQL
                     PTRQGFPFQLIAEQKYGYKWIRWINRIELSSDSEYLGYWESKGYSNSANLGEY"
     gene            14438..14785
                     /locus_tag="VKN64_02805"
     CDS             14438..14785
                     /locus_tag="VKN64_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMA59196.1"
                     /translation="MELLEIMKALSHENRLRILNLIKDEQLCVCEIEYVLDINQSNAS
                     RHLNKLKQANLIVGERKAQWIYYKINQEVIKEHKFLDLIIKQELKKITICQEDEIKLK
                     EYQQSGLSCQDLD"
     gene            14817..>15580
                     /locus_tag="VKN64_02810"
     CDS             14817..>15580
                     /locus_tag="VKN64_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HMA59197.1"
                     /translation="MTEELDITNDKDVQEETKEISFFEKYLSIWVGLCIIAGVLIGRL
                     WPAFPNTLNQWEYAQVSIPVAVLIWFMIYPMMVEIDFSSIKEAGKRPKGLTITLITNW
                     VIKPFTMLFFAWLFLELIFNPFISNALSQQYIAGAILLGSAPCTAMVFVWSYLTDGDS
                     AYTLIQVSVNDIVLVFAYAPLVMFLLGLSELTVPWNTVLLSVILYVVVPLTFGYISRK
                     YIINKKGKDWLENVFLKKLDNVTIIGLLLTLVILFS"
BASE COUNT         5411 a         2433 c         2469 g         5267 t
ORIGIN      
        1 tagttggaga ggcaggtatt aaagtcaatg aaattgcctg tcgcatcggg ggagcttatg
       61 aagcagactt tatgccctta attactgatg tagatatatt aaagatggtt attaacagtt
      121 ctttaggtaa aagtattaat aaagaaccat taaaaaacta taatttatta gaaaatgata
      181 actggttatc tgtccagtta tttttcgccg gtaaagggaa aattaaaagc ataagaaata
      241 ttgaacatat taaaaatatt ccagaagtag ttgatattaa agtaaactat aaagaaggag
      301 acactattcc cgagatagaa aacgccactg cccgggccgg ttattttatt gttaaagcta
      361 aaagcaaaag agagttaaaa aataatataa aaaaagtata tgataaatta aaaattttaa
      421 acactaaaga taaaaacctt gttttaagaa aagccggtga aacactttag tactgttttt
      481 ttgtatatat ttacattatt caaatttcaa taagggaaag ccccggagtt tataaaccag
      541 agctttccct ttttatatta agtcaaaata ctatactaat attattcact gattttttgt
      601 ctcatattta atttaagcta attatctatt accttatata tttttacttt ccaatctttg
      661 gttcctatat acttagcgat cctcattcca taagttttat tggaaaattc ttttgccaac
      721 ctacaatccg ttacaagata atccttcata tctgagtaat aaggataaag atttccatag
      781 agggtattcc aggttggtct tctattataa ataaaatcca tctcttccgg gtaaattata
      841 tattttatat tatttttttc gatatattca gagaaactta aattattttc atctaaataa
      901 gcaagattcc tgtaatcata taacttatta tagtcaaaat agaattgagt atttagatta
      961 gctaatactt tttcttcttt attaatggta gttgaaatat cattaagata atccttgtag
     1021 ttaaagtaag agttattata aattataata aaagaattta gtaaaagaat gaatattaaa
     1081 gcagttaaaa tagttttaga atagtccggg tcaatatttt ttattaaatt tgctaacaag
     1141 aaatagagga taggaaatat aaagatgatt gaaatttgat tatatctccc gatcattata
     1201 taacccaagt tgatccctat aaaagaaagc agcaaaatat taataaagtt atcttttttc
     1261 gtaaacaact tatatattga ataaactata gtaagcccaa aaagatagaa ttggggtttg
     1321 atatccgggg taaaataagt gccgctgacc tgataatata attttttata aaaataatcc
     1381 aaacggtcta atttgatttg tagggactta aaaacgccca gagtttcacc atagctttta
     1441 tagttgtaga taaaattagg atcgaaatat aagctcatta aaataaagat tagggcacct
     1501 ataaagaccg ttgttaagaa aactaataaa tccatataat ttaatttttc tgtaaaaata
     1561 atgtaataga ggtataaaaa caaaaaaggt ataaaagtaa taaagctgtt gggatgaata
     1621 ccgattgata ttgatattat taaccccaat aagaaattac tggaataata tttatcctga
     1681 attacttttg ttaaaaagta ataaagagcc attaaaaaga tagatactaa tataatttcc
     1741 tgtctcgcca gatgggaggt atatataaat tggatatcaa ctgcaagtaa aatggtagtt
     1801 aaatacgata attttttaga ctttgttaat atgaaagtaa ttctatagaa aaagaacaaa
     1861 gaaatagttc caaaaattaa agagattaat ctgaaggtga atatttcata gccgaaaatt
     1921 tttaaaaaac ctacttgaat tgaatgaaat atcaccctaa tagcatgtgg actgcggggg
     1981 taaaggtcaa aaaaaggttc tgttacatca taagttcctt cttttaatat agttcgagat
     2041 aaaccagcta accaggcttc atcggaatga acaaaaggga agctggtaag gaaaagtaag
     2101 ttagctagaa agtaaatgat aagaatcaca tatagtttgt ttaaattttt tagtttaatc
     2161 atatatatca gacctttaag tttactatta tttattataa cacaaaaaaa tttaggttaa
     2221 aggagaaata tttaaagaaa aatttcacat tatcgaataa tcaaatgcac tattaaaaat
     2281 gctaatacca aaaaccaatc tttatccctt ataatataaa taaagaagat tgtactttat
     2341 ttaacaatat tattttcaca aattaaattt gtgaaataat attatataag tacaagtgcc
     2401 tggaaataaa tatgtaattt aaaggcacta aatataataa aaaagagaca accaaaattc
     2461 taaggaggga aatttttaat gagaagaaac aaattattat cagtcctatt tatagttgct
     2521 ttgacattag tagttatgag ttcatctgta ttagcagtct cagatgcagt agaagatgca
     2581 gttaacggtt attttgccaa cctaccagga gataacgcta tgataggtca agaagcattt
     2641 gtagacaagt taaaagcagg agaagattta gttgtattgg atattagaca acctgatgta
     2701 tacaatgagg gacatgtaaa aggtgcagtt aatattccct ggggaccaga tgcaatgcca
     2761 gctgccctag ataaactgcc tggtgatgaa accatctatg tatattgtta tacaggccaa
     2821 actgcaaatc aagctgttgg attacttaac tttgctggtt ttgaagcaaa gtcagttaag
     2881 tttggttgga atttaggtat agctaaagta gacggttatg aagaagtagt agaaactaca
     2941 gccaatgaac tgggtggaga aaccgattat aaaattaatg aagatattaa aacagcagta
     3001 gttgattatt acaaaggatt agcagatgta agtgatacca tgtttaaaaa ctataaaatt
     3061 agtgaagata tgttaaagat ggctttagat tcagatagtg atctaatggt agtatcaatc
     3121 agacagcctt ccgcttattc agaaggtcat attgaaggcg caatcaatat tgcctggggt
     3181 aaaggcatgc aggaatattt tggtcagctg ccacaagata aaaaattagt agtatattgt
     3241 tacacaggac aaactgccgg acaagctgtg gctggattaa gaatgttagg ttatgacgct
     3301 gtatcactta atggtggtat gggaactccc gctaacgagc cttatggatg gtctaataaa
     3361 ggatatgaaa cagttcaata attaacatat aattaacata taaaataata taataaagca
     3421 aaagctggca gttaatttta gctgccagct tttataatat cattttatat ctttaattaa
     3481 ttgtattaaa ttaattttaa acttttcatc atctttttta cttctctttt tagctgtggg
     3541 atgtctccca cctgcccttc ttattttttt attaatatgt cttttcataa ggagggtatc
     3601 tatattctta tctgtatata ttttgccttt ggaattaata gcagaggctt tcttagaaag
     3661 ggctaaagag gcaagcccat aatcatcggt tattatgata tcctttcttt ttgcaaagtt
     3721 tatgatcttc atatcaaccg attgataact ttgatctact ttcactaccc taccatagtt
     3781 taattttata tcatggttat aatcgcaaaa taatattaat tctatattaa atttatgagc
     3841 aatagattcg gctagatcta tcactggaca ggcatctcca tcaataataa tgcgcataaa
     3901 taactctcct tttccattcc gaaaaattca tcttctttat tattataact taaatttggc
     3961 attttttcta ttatatacta tgaaaacaat atgagcatat gttataattg ataatgaaat
     4021 atattaaagg aaataacttg aagttattga atataaaaaa taggtttact ctttttattg
     4081 tctaaaatct tgtagtaccc aatttataat acagaataag agtaaagatt ttttttgcct
     4141 atattgttta agttagtttt atacttatat acaatattta agtgaagctt aaaatgtatt
     4201 attaagtaat aacaaggaag ggtgtactca tgtatataaa gagtcttaag tactttcaag
     4261 aaattgccaa aataaaaagt atatcaaagg tggcaaacaa ttcccatatt tcacagcccg
     4321 ctttaagcca acaaattcaa aaattagagg attttttagg gacagaactt tttattagaa
     4381 gtaatagagg ggttgagtta actgaatccg gaaaaattgt tttaaagtat gcggataata
     4441 tcataagaac ctatgataag atgctctctg aattagatga gcaggatagt aaagagataa
     4501 aaattgaagg tgagcatact attgctactt actgtttacc ctgtgtaatt ttaaatatgc
     4561 agatgaaatt tcccaagcat agatacaatc tagtttctgc ctctcccgat aaaatagaac
     4621 aggatgttgc aagtgatatc tgtgaaatag gttttactac cacacccgtt cgagacgaaa
     4681 gtttaaattc taaaaatgta atcgatgaaa aggttgtatt aatttcgccc ccttcttatg
     4741 atatgcctga aaaaattaat ttaacagatg tattggataa taagttcatt atcttaaaag
     4801 aagactgtat tattaaagaa aactttaaag atggactcaa agaaaagggg catagtttta
     4861 atgatgtaga ggttatatct cgccttgatt ctacagaagc tatgaagact ttagtgagaa
     4921 aaggatatgg agtagctttt gttccttata attcagtcag agatgaattt agtgctaatg
     4981 agattcgagt atcaagaatc actgattaca accttgatta tgatatttac atgatttata
     5041 aaaaagcaga atttttaagt aaagaagcga aggaatttat tagacatttc aaacaactcg
     5101 gaaaacatgt atgttattaa attaacagga ttaaaaagtg aaaaaaacaa tacatttgat
     5161 aattgaatat actattaata aaagttatga caataaacag tgattatatc atataatatt
     5221 attaaaggtt gtactatttt taacaatata gaactggagg ttgaaaaatg aaaaaaatag
     5281 caaagataat ttttgtttta ttaacatttg cgattatttt cacaggctgt gaagcagata
     5341 ttaccttccc tggggaagta gggcaggata taattacagc aaatgaagca ttaaaaatga
     5401 ttgaaaatga agatgttgta gtagttgatg cgcaaaaatc aggtgattat gaggatcaac
     5461 atttagaagg tgcagttaat attgccagaa atgatatcac tacctttgga cctttcccca
     5521 atatgctggc ttccgcttca aaaatagaag agactttagg tgaaaatggt atttctaata
     5581 attctaaagt tcttatatat gataataaca ataatatgga tgcagcccgt ctttggtgga
     5641 caatgttagt ttttggacat gatactgcca atatgaaagt aatcagtggg ggactaaatg
     5701 cacttcaaga tgaaggggca agttttgctt cgggtgaaac tgaagttgag acagttgaat
     5761 ataatgcttc tgagaaaaat gaagaatatg ttgccaccaa agaagaagta ttggccgaag
     5821 taaatgaacc cagtgaagat actgtaattt tagatgtaag aactacagaa gaagttaatg
     5881 aaggtattat tccgggtgcg gttcatatta attatatcga taactgcaag gataacggtg
     5941 aatattatcc acccagctat atacaaagat attatcctga caatgaagtt aaaccaaacc
     6001 aaaaagtaat aatgtattgt aaaacttcta ttagagctgc ccagaccttt ttagcattac
     6061 ataatgcagg ttatagaaac ttagagattt atgatggagc ctggattgaa tggtcatcag
     6121 atagttcttt acctcagggt atgccaagcg gagaaccagt tgaacccagt caacaagatg
     6181 cttcgtaaaa aaattaataa ttaaagatta gaattaaata aaaagtataa aaatcatggg
     6241 ataaaaaaga ttaatagttg ttataatatt aatgtatgta tttgcaattt ccagatcagt
     6301 ctttgtatga aaggtaaagg ctaaagaaga tcttttcgta tcagatttca gacagctttt
     6361 cgcttattta gaaggtcata ttaaagccgc aagcaatatt ccatggggtg aaggaatgta
     6421 gttatatttt ggttagctac cacaagatca aaagttagta gtttattgtt actcaggcca
     6481 aactgacgga cagacaatag ctagattaag aatgttagtt tatgatctgt atcacctaat
     6541 gttggtatgg aactctcgct aacaagtctt atggatgttt caataaaggt aatgaaactg
     6601 ttcaataatt aacatataaa atataataat aaagaaaagg ctggtagttt gatgtaaact
     6661 accagccttt ttaatatatc ttacaataaa tattatctac aaatattgaa ttaattctaa
     6721 tctattcttc tatttgactg tggattatgt ctaaaatttc attttttacc tcaaagtaat
     6781 tatctttata tattacatct ttagttccct cttcaaatat cctttttgta aaatcgacat
     6841 caaaaatttt aataatttca ccaggtagtt tggaaagtac aactactctt gtcgcaagag
     6901 aaagagcctc atccacatca tgagtgataa aaaagatagt tgtattattt tctacccaaa
     6961 tatctctgat caagttctgc atattatctc tagtcagggc atctaaggcc ccaaaaggtt
     7021 catccattaa tattacctga ggatagtttg ctaataccct tgccaaagca acccgttgtt
     7081 tcataccacc tgataattca aaggtatatt ggtccccaac tccacttaga ttaacttcat
     7141 ctaaaaagta ttctctaatt tttttaattt cttctttcgg tagatcgcgc attttaggtc
     7201 cgaattcaac attatcttta acagacatcc aggggtataa agtaggggat tgaaagataa
     7261 ctccccggtg ccagtccgga ccggtgattt ctttaccctg cattaaacag cgccccgagg
     7321 agggttttaa atagcctgca atggttttaa gcagggtact tttgccacat ccagagggtc
     7381 caagtacaca gacaaattcc ccccgcttta tttccaggtt gatatccttt aaagcaagag
     7441 ttgatttctc ctctttacca tatagtacac tcatattttc tattttaatt aaagtttctt
     7501 tttcagtctc tacagtcatg gattaaaccc tttctcttga aactttactt attattcttg
     7561 ttcattatct aaagacattt ttatatattc gggatttaca tattcattga aagtttcttg
     7621 tgaaggcgat acttcaattg aaccttgatt ttggagaaaa tcagaagtat ctttcataac
     7681 tttagcaaag ttgcccggat tttctaccgt tccaaagaag tcctcactta aaagttcttc
     7741 ccgtgttttc caacttgtac cacgcatttg atgtaaggct acttcggctt caatacctaa
     7801 ttctccggaa acaatttcag cagcttcttg aggattttct ctataaatat cgcctgcttc
     7861 tgccatagct gagataaaat cagccactag atggggatat ttattagaga attctttacg
     7921 aacaacctcc acattggcag taatataacc tttttcagcc atatcttcac ttgttatcaa
     7981 ggcccgccca ttatctaata attttgctaa agtaggctgc caactataag cggctttaat
     8041 atcgccgcgc tcccaggcgg caactatttc agcggtctgc atatctaata gctgaacatc
     8101 atcttcaata cctgcttcct tgagcacatt tagaagtata taatgagcag tagaagcaaa
     8161 gggtactgcc acccttaaac ctttaagatc ttctattttt tcaatacctg aaccgttttt
     8221 aacagctaat gcttcattat cacctaatac ttcatgcagc cagaccattt caactttaag
     8281 ttctctggat aaagctgtaa tagcgttggt attacccata gttgcaaaat caatactacc
     8341 cgaagcgaga gcttgatttg cttccgcacc tgaatcaaag acagtagtat taacttcaat
     8401 acctctatct gcaaaatatt tatcaaaata tcctttcgcg atagctatgg tttcatcatt
     8461 agggatcctt aagataccaa agttaatttc ttccggtagt acttcttgat ttccatcttg
     8521 tccagattga ttatttccac acccggacag ggaaaatact aaaagtccaa ttaataagac
     8581 cacagtcata atttttataa aagaattttt cataataacc tccgttaaaa tttgataatc
     8641 aatacttatt atagatgtcc tttccagaaa ataatgtttt cttctaaata tcttaatccg
     8701 gcatcaataa taacaccagt tatacccatg ataattattc cgacaaaaat aacttcactt
     8761 tttaaaaagt tcgaagcatc caaaaccatc cagccgatac cagaggtagc agcaaccatt
     8821 tctgctgaaa ccagagttgt ataagctacc ccgaaggcag ttcttatacc tgtgaatatt
     8881 tccggcatag aggctggtaa tactatttta aagaagatgt ctttttgttc tgcccctaag
     8941 gactctgcac ttaacacaaa gtctctatta agctttctca cggccgagac acaggctata
     9001 taaataggag caaaggctgc taaaaataga agtacctgct tggagagatc gtcaatacca
     9061 aaccaaagga ccaaaagagt atagtatgct aatggtggta atggtcgata aaattctatt
     9121 acagagtcta taatagcctc tactttacta aaataaccac tcaaaagacc taaaggaacg
     9181 gctgtgaata tggcaaaaaa tagagcaacg aagagacgtt taagactggc tcctagatgg
     9241 acccagatcg gtactttatt ataaccgttt tccaaaatgt gtaaaaaggc ctgccaaact
     9301 ctgaaaggac ctggcagcag agtaggagaa aagatttcta attttgtaat cacataccac
     9361 aagataagta tggaagccca ggttgtaaaa gttaggattt tttctgtttt cgttttttta
     9421 tcttcatcta cccgcctatt tattaattta tctgcccatt tagttttatt tttgttttct
     9481 tctttaggct gctcattatt ttttagagcc atatttttac acgcccttat ctataatctt
     9541 aaaatatatt tttaatctaa ttcattttgc taacaaaaag atatcatatt tatctttaat
     9601 tatcaattgg atttgcattt aatgaagtaa agaccatcaa gtattattac ttgatggcct
     9661 tatagattga gagttaattt atatttactt taatgtattg ttttttactc aaaattatac
     9721 tctgttacta ttttatctct atcagtagtt tgatctaaga agtattcata gcagcagatt
     9781 cctaagaatg agcctgaaat gtttactaca ttgtcatagc ccataccttg tagagctaat
     9841 actgcgttat aacttctctg tgctgaacgg cagtgtaagt atactggttc atcttcggga
     9901 atttcatcag ttctacctct tagttcacta agtggaatat ttacagcgtt taccagatga
     9961 ccttcttcat attcgtcttc ttctcttaca tcaattataa aggcatcatt ttcaacaagt
    10021 tctcttactt tagtaactgg aacctgatta tatctattat tgaggatgtt attagcaacc
    10081 ataccagatt ggtttacagc atttcttggg gtactgaata cgggtgcata acatagttct
    10141 aatccaatta gatcttctac ggttgcatcc atcataattg cggtagcgat gatgtcaact
    10201 cttttatcta cattaccttt gcctactgct tgagccccta atactttgcc agtgggagtc
    10261 tcataaagta gtttaaagtg taagggatta ctattaggca ttaatcccac tttatcctgt
    10321 ggaataatat aaacaaaatc gtatgagata ccagcatctt gtagttgctt ttcatttaag
    10381 ccggtagttg cggcattgta attaaaggct ctaattactg aggagccaat aacaccattg
    10441 tttttatgtg gtatattgta gatatgatta gccgctgctc ttgcttgtct ttgtgctgga
    10501 ccagctaaag gcattcttgt tttactgcgt gaaatacggc agtattcttc aattacatcg
    10561 cccactgcat aaatatcagg gtcactggtt atgtaattat gattaacctt aattccgccg
    10621 gtttttccaa tttctaagcc agcatcttcg gccagttgag tttcgggatt aacaccgata
    10681 gataatacta cagcaccggc atctatcttc ttaccggatt ttaagacgac atggtcatca
    10741 tgtacttctg acagtggatc acttaaaact acattaactc cattgtcgtg taattctttg
    10801 tgtacgatct gggccatgtc ataatcaaga ggctgtagaa cctggtctaa tgcttcaatt
    10861 aatgaaacgt ttttattaga atggataaag ttttcagcta tttctattcc gatatatcca
    10921 gcaccgataa ctgcaacatt ttcaatatta tttttgttaa tatactgatt taatttatct
    10981 atgtcaacaa catttctgat tgtaaagaca ttatcattgt ttacaccttt gatactttgc
    11041 ggtctaatgg gatttgcgcc aggagagagg attaatttat catagtcttc ggtataaacc
    11101 tctccgcttt ctaggtcttt tacttctacg gttttttcat ttctattaat tttggttact
    11161 tcatttctaa ctcgggcatc aatattatac tgttcagcaa agttttcagg acacattaaa
    11221 actaagtcgt cacattcttt aacaacccca ctgatgtgat aaggtaaggc acagttagaa
    11281 aaggaaacat gagggccgcg ttcaaacata acaatttcgg ctgattcatc taatcttctt
    11341 gctcttgtgg cagcagaagc tccacctgct actccaccaa caactaaaat ctttttactc
    11401 atttttaaat tcctcccgtt tctttaatta ttaagcctag gtttctattt gaaacaaaat
    11461 attagcctgc gattttttca ttgaatttgt tactaattat acctcccaca atcatgaaga
    11521 ccaaaaagag ccaacctgat aatgagaagt tagcaatcgg tgtatataat gctcctacat
    11581 tacaaccatt tgctagacga gttccaaaac ccattgttaa gccaccgagt gcatagatag
    11641 ctgcctgttt tttagtgatg ctaagttcag aagtgaacat ctttgaaaat ttaccggcag
    11701 ttaatagata gataattgca ccggtgataa taccgaagtt ttgtactgaa atctgatgtt
    11761 caaaaaatgg tgtattaaat acttcggctg acattctggt aaaactactt aagctttcag
    11821 cagagatgcc gaagatcatt aagaatttac cgaaccaaat accataaggt tgagaagcac
    11881 cccagcctgc tctggttact gccattaata atgtgaagat tatagctaag gcaactgctc
    11941 cctgttttaa tgtccagggt ttttcgaaga aatggtagta tgtatcagca ctaaatagtt
    12001 tgaaattctt aaaatctatt gattctgcat tttcttgaat tctttctatt tccaatccgg
    12061 tatatttatt atttttaacc tgatatcttt catatagata ggagagagct gtaaagatta
    12121 cacataaggc tcctaatgct attaaaccgc cgagataacc ttcaaaacca tctccctgga
    12181 aaaagtcggg taagaaaact cctcccatag tccgataacc tatataagaa ctaaaccatg
    12241 attcctgtac ccaacttgct gtattttgaa ctggaaaacc caaaaatact cccaaaccaa
    12301 agaagataag ggttgtaaaa gctctcggga agcccatggg taagtcggtt aggaccccac
    12361 ttgcacaaca tacagaaagt gacataccga aaccaaataa aaatccaccg aaaataacac
    12421 ctaaattaat tggttttacc cagaggttaa aactggctgg gtcatttccc aataaaaggc
    12481 cggctgttaa tacagctgtt atgaaaaaca taaacattaa agttctcatt aatttagtag
    12541 atccggtcct atatgctctg tttacactac ctgcaaaacc agtataagct cttgcaaggg
    12601 catatccaat acccgtacct attaataatc taaaaaatag tatatctgtc ttgagccaga
    12661 atttagctgc gattaggatt actaaaattg caataaatcc tataaacttt tctgatttct
    12721 ttttcatctt ttttataatt ccctccttta aaatctccaa gccttcaaga ctttgttcta
    12781 aaattctcaa agcagggcaa aaagtaaatc cctcgcccgg gatgaaagct tagattataa
    12841 cttataattt tattcacaaa gttgtgtatc tatatatagt atatactatt tattaacaat
    12901 actaaaataa taatatctta tgcaggataa tattatttta tggaagaatc gttttgtgat
    12961 aaaaatatct aatatgattc ataatctaat agttaattta ttaatttgcg ggagatataa
    13021 atttgttgtt ataaaatgat tgtgaaaagg tatattaaat ttaaagatta tagaaagaag
    13081 ttcataaaat ctaataaaaa tatgggcctc ttcaaaaatt gattgagaat taacctcaaa
    13141 aaaaaagttt ataacagtaa tccttatttt atgtatgcag atgtaaattt ataaaaagat
    13201 tttaaatata gataaaaaat gcccccaaat tttttatggg gacattttgt taaaattgat
    13261 attagtattc tcctaaatta gctgaattac tatatccttt tgattcccag tagccaagat
    13321 attcagaatc actggatagt tcaattctat taatccatct tatccattta taaccatatt
    13381 tttgttctgc tattaattga aaagggaatc cttgtctggt aggtagttga acatcattga
    13441 ccttatccgc aatcataata ttattttcca taataaatga caatggtaaa gaagttgtat
    13501 aaccatctgg tgaatagaag attactgtat tagcttcatc cttaacttcc acttccttaa
    13561 aaatactttt taaaggaatt ccttcccaca atgcttttac agaccaaccg gttacacagt
    13621 ttaatgttat taccttttta atatgttctt tctcttgcag aactgaatat ttgtattcct
    13681 gcttgttatc aactaatcca tctacaatta atctgtattc ctctatgtcc acttcctgaa
    13741 tacctttaat cgaattctct ctaaaatctt caattgaacc taacttttca ccttcatatt
    13801 catttatttc aacttcttgg atatcttcct gagcgagtat agttatatta aaagagatta
    13861 ttagaattag aatagttatt atgataaaaa atttatattt cattaatgaa gccccctctc
    13921 tattagaaaa tatatttata tataatattg gttatataag agatattata gtatatctat
    13981 tattttacta tgttttttta attaaatcaa ttaataataa ctattactaa ttataataca
    14041 gctttaaaat taaatacatt ttctaatctc acaattaaat taacaggaag tataggtcca
    14101 attcattgtt ttaacaacat tttgtccttt ctattaagta ctatcaatgt tttttatttt
    14161 gtgaatatat tttgttttaa tttgcctttt aatattattt ctaatctttt aataggtcaa
    14221 aactaccaaa ttgagtgaga agtaatattt cttcttaatc tttaaatttt tgatgatatt
    14281 aatcaaaaga tcaaaaaaat acttatcatg aatttttgac ctttttaatc agtaatttaa
    14341 tgaaatattt aagaaatata attgacatta ttatgttgtt tttatataat atgagtaaat
    14401 tcgattatac tcatataaaa agaaagaggg attataaatg gaattgttag aaataatgaa
    14461 agctttatct catgaaaaca gactacgtat cttaaattta attaaggatg aacaactatg
    14521 tgtatgtgag attgagtatg tattagatat aaaccaatca aatgcttcta gacatttaaa
    14581 taaactaaag caagccaacc ttattgtagg agagcgaaaa gcacaatgga tatattataa
    14641 aattaatcag gaagtaatca aagaacataa atttttagac ctcataataa aacaagagtt
    14701 aaagaaaata acaatatgtc aagaagatga aattaaatta aaagaatatc aacaaagtgg
    14761 tctgtcctgt caggatctag actaatcaat ttaaataaaa ggagagggag tattaaatga
    14821 cagaagaatt agatattaca aatgataagg atgttcagga ggaaactaaa gagataagtt
    14881 tttttgaaaa gtatttatcc atttgggttg gtttatgtat aatagcgggt gtgttgatcg
    14941 ggagactgtg gcctgctttt cctaatactt taaatcaatg ggaatatgca caagtttcta
    15001 ttccagtagc agttctcatc tggtttatga tctatcccat gatggtggaa attgatttta
    15061 gtagtattaa agaagctggg aaaaggccaa aaggtctaac tattacatta attacaaatt
    15121 gggtcataaa gccctttaca atgttatttt ttgcctggtt atttttggaa ttaatattta
    15181 atccatttat ttccaatgct ttaagccaac aatatatagc aggagcaata ttattaggtt
    15241 cagctccctg taccgccatg gtttttgtat ggagttatct tacagatgga gattctgctt
    15301 atacattaat tcaggtatca gttaatgata tagtcttagt atttgcctat gcaccgcttg
    15361 tgatgttttt actaggatta agcgaattaa cagtaccctg gaatacagtt ttattatctg
    15421 taattttata tgtagtagtg cccttaacct ttggttatat ttccagaaaa tatattatta
    15481 ataaaaaggg taaagattgg ttagaaaatg tatttttaaa aaaattagac aatgttacaa
    15541 ttataggact tttattaacc ttagtaattt tattttcctt
//
LOCUS       DATGCJ010000057        18062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_319772, whole genome shotgun sequence.
ACCESSION   DATGCJ010000057 DATGCJ010000000
VERSION     DATGCJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 18062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18062
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319772"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VKN64_02815"
     CDS             <1..219
                     /locus_tag="VKN64_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HMA59198.1"
                     /translation="LSAIPLPDPDREANKKPLEGEIPSPVNPPSGCRFHTRCWLAEER
                     CRREEPEFKEIKDGHYVACHVMEERYSD"
     gene            246..1427
                     /locus_tag="VKN64_02820"
     CDS             246..1427
                     /locus_tag="VKN64_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA59199.1"
                     /translation="MKTKKIQNEIKKIEEDIVKLRHLLHQYPEIGYEEYNTAQLIAKT
                     LKKLDLEVNDDIYATGVSGLLKGKENNKTILLRADMDALTIQEKTDLPYASQKDGIMH
                     ACGHDVHSAIVLGAAVVLKELEEELPGNVKFVFQPAEEQEGGALGMIENGVMKNPKVD
                     AALGLHVWGSAKKGTVEIKYDSMMASPDRFLIKIIGEGGHAANPHQCVDPIIIATEIV
                     QEFQNIISRKVEPTEPAVLSVCHIEGGNTHNVIPDEVIIEGTVRTLTEDVRDSIPSLM
                     EKMLTKITEIYGAEYELDYDYSFPPLVNNKEMTDLVRNSANKVMGEDKVIEINKPNLG
                     GEDFSYFAKEVPSSYFYLGIAPSKENVIKHHNPYFYVDDDVIADGIAILVQAVFDYFD
                     N"
     gene            1500..1916
                     /locus_tag="VKN64_02825"
     CDS             1500..1916
                     /locus_tag="VKN64_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HMA59200.1"
                     /translation="MKVAVIDGLGGGLGAQIVEKLNNKLSNKIDILALGTNAVATSKM
                     LEQGADEGATGENAIKLNVNKVDLIIGPLGLIIPNSMKGEITTVMAEAIAGSDAKKII
                     LGMRQPHVDLVGLSDKTLNELIDDSIKKTEEFLKGK"
     gene            2016..2561
                     /locus_tag="VKN64_02830"
     CDS             2016..2561
                     /locus_tag="VKN64_02830"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HMA59201.1"
                     /translation="MDIKKIVYTAFFIALGIVLPMVFHLVGGPSLGRVFLPMHLPVLI
                     GGAFLGPMAGLIIGIITPLLSSLFTGMPPVIPMLPIMIAELGIYGLVMGYFFFRIKMN
                     VYLSLIISMLAGRIMASLVVLALVYGFGFARLPANPIIYIYGTITAGFPGIIGQLLIV
                     PLVLRYIKRFNEQKPTLETAY"
     gene            2653..3630
                     /locus_tag="VKN64_02835"
     CDS             2653..3630
                     /locus_tag="VKN64_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HMA59202.1"
                     /translation="MPANLPPQYYEAEEVYRNAQTTEEKIAALREMLSIMPKHKGTEK
                     LQADLKKRISKVKEEKKKKSKSKSTYNPYLIDKEGAGQVILLGSPNVGKSSILDSLSN
                     AKVEVAEYPFTTSLPLAGMVEYEDIQIQLIDTPPLVPEDVPGPMISAIFHADYITIVI
                     DASADDCLDQLSGIIEFLEGKRIIRDEIPENVKGYHLEDILVLATKVDLSGSKDNIEI
                     IKELYPNLDLLKISNKTDQNLDKLPKILFNKLNVIRVYSKAPGEEPDYDRPYILDKGE
                     TVLDFAQLVHKDFAKNLQKARIWGSAKFDGQHVAKDHVLEDQDVIELHT"
     gene            3659..4075
                     /locus_tag="VKN64_02840"
     CDS             3659..4075
                     /locus_tag="VKN64_02840"
                     /inference="COORDINATES: protein motif:HMM:NF020543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HMA59203.1"
                     /translation="MEEKQLAINELNNTDYSLVVVKNSKVEYRSKEESVGSIVNLLEN
                     RPELLKNAVVADKIIGRAVAMVCDFASVNYCYGQIVSKGAVDVFENNNLSYKAAEVVE
                     AIKNRDNTDLCPIEKLTLDINDSAKGIEKIKEFLNN"
     gene            4094..4507
                     /locus_tag="VKN64_02845"
     CDS             4094..4507
                     /locus_tag="VKN64_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HMA59204.1"
                     /translation="MGSYIKIYILAFIIFMGIDLVWLGILAKDFYRSQLGTFLKESYN
                     MKAALLFYLVYIAGLIFFVVSRAIATGNWQYAVFAGMFYGFVTYSTYGITNYSTIKDW
                     PTLLTIIDIGWGTVLCGLTSYLTYLIASNFSFFLN"
     misc_binding    4671..4884
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5037..6281
                     /locus_tag="VKN64_02850"
     CDS             5037..6281
                     /locus_tag="VKN64_02850"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMA59205.1"
                     /translation="MSKNNKKIEKILLAYSGGLDTSVSIKWLKEKFAAEVITYTADLG
                     QKQDWKKVKEKALATGASKAYIDNLEKEFVDNYVIKGLKANALYENKYPLATALGRPL
                     IVEKMVDIAKKENVDALAHGCTGKGNDQVRFEVGFKALAPELEVIAPLRDWEFKSREE
                     EIEYAKENQIPVDATKSSPYSIDQNLWGVSIECGLLEDPWNEPPADAYLWTKDPHESP
                     DNELKLEIEFKKGKPISLNGTIMDTKDIIKKLNEIGGQYGIGRIDLVENRLVGIKSHE
                     IYETPAAEILLKAHIALEELTLDKETKQFKNMIENKYASLTYNGLWFSPLKKHLDKFI
                     EETQQNVSGIIKLKLYKGSCTVIGRKSPYSLYNYGLATYDQNDQFDHNSAQGFINLWA
                     LPIQIANNVDSYIDKNSKEAVK"
     gene            6278..7675
                     /gene="argH"
                     /locus_tag="VKN64_02855"
     CDS             6278..7675
                     /gene="argH"
                     /locus_tag="VKN64_02855"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMA59206.1"
                     /translation="MKLWGGRFDKNTTLRMDKFNSSLPFDIRLLPYDIQGSIVHVKML
                     AKQNIIKGEEKEAIIKGLKEIEAELEEDISNNKFDYYEAEDIHSLIEKRLTGKIGSTA
                     GKMHTGRSRNDQVNLDLKLYLNEITEKIKELLLNLMEILVDMAENNHDVIMPGYTHLQ
                     RAQPITFGHHILAYFYKFKRDLERLEDYTTRLNISPLGSGALAGSNFNLDQEFTAEQL
                     GFAGIYENSIDGVSDRDYVLEFEYIAANIMIHFSNISEEIIIWNTSEFSFIELSDEYT
                     TGSSIMPQKKNPDLAELTRGKSGRVFGNLMQMLTVMKGLPLAYNKDLQEDKEGLFDTC
                     DTILRILEVYPDMLKTMRINKEKLKEVVENDYLIATDLADYLTSKDIPFRKAHELVGE
                     IVKYAEDNKKNLKQLKFAEYNNILGQYHEYIDKDIYKLFDLEESISSKKTAGGPSNKR
                     VKEQIRQARNFINIK"
     gene            7791..8375
                     /locus_tag="VKN64_02860"
     CDS             7791..8375
                     /locus_tag="VKN64_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59207.1"
                     /translation="MDEIRDELIKCGTTLFSKKGYRDTKVKDVTDLASISVGTFYNYF
                     DSKEELFLDVYLRKHNRAKQELLDFFKEDKPPLKIIDKAVHRFLDMMQTDPILRAFFN
                     PNIHEKYRQSLDIKRKKTHLNYAYDLFAPQLEKWQDRGEINEEVDIKLLLAVFDSIFY
                     VLLHKKDIGEEFFPEIVDFLIDSVLCKMNKNFYQ"
     gene            8829..10652
                     /locus_tag="VKN64_02865"
     CDS             8829..10652
                     /locus_tag="VKN64_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59208.1"
                     /translation="MKRSSILILVLSMFFVLSMSIGAFAQVNNPTTFTKVNMGTIDSL
                     DPHYQYDNASAEIYANIYENLIMFDEGDVTQYKPMIATEVPTVNNGLVKDNGTTYIFP
                     IREGVTFHEGGTLTAEDVKYSFMRGLIHDRNGGPWWMLYGPLFDAGGLSDITNEVVGV
                     EDPSQLTAEQSAEVFEVLDKAIEVDENNVIFHLPKPNPTFMSVIVHDNGLGAIMDKEW
                     CIEQGAWDGSPETIAEYYDPAKEEDPLHENTNGTGPFMLNTWEKGKRIALDRFDGYWR
                     EPAQLQRVIINYIDEWSTRKLMLQRGDADVVYMDKQYQSQVEDLEGVELITGLPNLYT
                     GYGLMNWTIETKGNPDVHSGQLDGKGIPSDFFADIHVRKAFNYSMNYEAFVQDVMDGD
                     GQQARGPIPEPFLGWSEDSSIYTTDLAKAEEEFKKAFDGELWEKGFEITILYNSGNSM
                     RKTATDMLKYNIEQINSKFKVNTRGIQWATYLDRMIAGKFTLGFIGWGADYADPHNFT
                     VPFMRTDGTFGNFKGENYKTWAEENNVDELVNKGVEVTERAERLEIYKELQHMTYEYA
                     TDMYLYQPTAHILKREWVKGWYYDPILHDGIYYYSIHKAAE"
     gene            10747..11778
                     /locus_tag="VKN64_02870"
     CDS             10747..11778
                     /locus_tag="VKN64_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59209.1"
                     /translation="MISYIVRRILILPIILIGVTLLVFSMIMMLSPYQRLSTYIKDPA
                     QLKSVDVDVLVARHGLDDPWYQQYGRWISGVARGDLGWSESAGATVSEAFLSRFPATA
                     ELGLFALLPVVLGGIILGVFASVHHNDFWDHVSRVLAIIGWSVPTFVFGLVMLMIFYG
                     HLGWFPPSRLDSWALEIVNSSGFINYTNFFVLDGFLNGNLAIVGNAIRHLIAPVVTIS
                     ILWWAFIMRITRSSMLEILRKDYIRTARAKGVTENVVVYKHAVRNALIPVVTVAGRMV
                     LGLLGGLVITETIFNIEGLGQFMAQAAQQLDYPAVLGGTLYFGFLLIMINLCVDVSYA
                     VIDPRIRLE"
     gene            11784..12659
                     /locus_tag="VKN64_02875"
     CDS             11784..12659
                     /locus_tag="VKN64_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59210.1"
                     /translation="MEWQEVTLRLFKNKISLIGLILVIGFGLIAVFAPWIAPPADPSE
                     PYMVPRAGWGFEPKPPSAEHIFGTAEGQYDIFYGIIWGTRTAFRIGFIIVGISTLIGI
                     AIGTLAGFYGGWFDEIVMRVTDIFMAIPFLIAAIVLTTILGQGLRNVMIALITFGWMN
                     TARLMRSQVLSIKEEEFVMAADALGAGDVRIIFKHVILNTIFPLVIEASMRMGSMVIT
                     ASTLSFLGIGAPEGYADWGQMISFARNWILGGQGNAIQYWFTVVIPGSAITLFCLSWN
                     LIGDAFRDILDPKLQ"
     gene            12680..13687
                     /locus_tag="VKN64_02880"
     CDS             12680..13687
                     /locus_tag="VKN64_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59211.1"
                     /translation="MAENKLIDVNNLKTYFHTEEGTVKAVDGVSFEIYPGETLGVVGE
                     SGCGKSVTSLSIMRLIESPPGKIEEGEILFNGKDLTKLSQGQMRKIRGNDISMIFQEP
                     MTSLNPVYTVGDQIMEAILIHKNVNKKQAREEAINMLRKVGIPLPEQRVDEYPHQLSG
                     GMRQRIMIAMALSCDPQLLIADEPTTALDVTIQAQILELMNSLKAQFGMAIMMITHDL
                     GVIAEVSDRVAVMYAGKIVEYTDVKTLYADPKHPYTWGLMHSIPHIEKDLDRLEAIPG
                     NVPNPLDFPDGCKYNTRCPFATDKCRVDEPDIEEVEEGHYSACWHVDKLEAKKSEEKG
                     A"
     gene            13703..14689
                     /locus_tag="VKN64_02885"
     CDS             13703..14689
                     /locus_tag="VKN64_02885"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HMA59212.1"
                     /translation="MAVEQERLLEIKNLRKYFPVKEGVFRKTVAHVKAVDDISFYIKK
                     GETLGLVGESGCGKSTAGRTILRLLEATEGEIWFEGKNLLSLDKKELREMRKEMQIIF
                     QDPYASLNPRMTVADIVGEPIDIHNLASSKKERNEKVADILDTVGLGPEYMKRYPHEF
                     SGGQRQRIGVARALAVDPTLIIADEPVSALDVSIQAQVINLLQDLQKDFDLTYLFIAH
                     DLSVVKHISDRVAVMYLGKLVELADKKELFDDPKHPYTQSLLSAIPVADPTYEKERII
                     LEGDVPSPVDPPSGCRFHPRCPYAMDVCSEKEPEFKDYGDEHFAACHLLEQE"
     gene            14911..15759
                     /locus_tag="VKN64_02890"
     CDS             14911..15759
                     /locus_tag="VKN64_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA59213.1"
                     /translation="MKEKIAVVTDSTCDLSSEILEEQEIEFLPLKIIYENNQYKDRVD
                     IKPQTVYDNFEDEIPSTSMPTPSEVREKYLELHEKGYTHIISIHISSGLSGTYNICQM
                     VADQLDEITVQVIDSKMLSMGLGRLVLYTKELIDSNQHSFNEIVDKVETKKEEIDLYF
                     IVDTLKYLKKGGRIGKVRGTIAELLNIKPIISINEEGEYFTFDKVRSRKKSITKLINL
                     AKEKINEGFCYVDVMHADAKEEAVDILDKMKDLPNVKDTVLGEISPAMVVHSGPGLIG
                     VCITKI"
     gene            15909..17849
                     /locus_tag="VKN64_02895"
     CDS             15909..17849
                     /locus_tag="VKN64_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA59214.1"
                     /translation="MTSYNHLKGEESPYLKQHMTNPVDWYPWRKKAFKKAKKKDLPIF
                     LSIGYSTCHWCHVMEEESFTDQEIAEILNENFVSIKVDREERPEIDKLYMEVCKVMTG
                     SGGWPLSIFMTPDKKPFYAATYIPKNDKYGRRGLLSILPQIKKLWDSQREKLLSTANE
                     VVNHLNKNESFDKVDIDERAIEKAAGILDRIYDKKYGGFGNSPKFPMPHYLLFLLHYA
                     KNSNNDKYLNMVETTLQNMRAGGIYDQLGQGFHRYSTDQKWEVPHFEKMLYDQALLIY
                     TYLETYRETNKNFYRDIVEEIIDYLKRDMLSETGGFYAAEDADSDGEEGKYYYWNLSE
                     LENILTKEEYDKFLSVFTIDDEKKINLRLKNVKDYKSITNIKQKLLDEREKRTRPGRD
                     NKILTDWNGLTVAALAKAGFVLNKEEYIKLAEKTLSFIDKKMKSDKGKLAHSYCNGKV
                     TEYSNLDDYSFYLWGLIELYQATLKEIYINKAVEIAEEMIKLFWDEKGGGFYYTTKEN
                     QELFLRQKDTADSAIPAGNSLAGYNLLRLAQLTDNMRYRSITETLIEALSKDIYNNPV
                     NHIFLLFSYKYLQEPFKEIKIIGDLNEEKAVNLMNKIRNNYWPELLIKHEQKEEKIMF
                     SLCKDFACKPPTEDIEEILREL"
BASE COUNT         6399 a         2510 c         3650 g         5503 t
ORIGIN      
        1 ctttcagcta taccgttacc cgacccggac cgagaagcaa acaaaaaacc gctggaagga
       61 gaaataccga gtccggtcaa tccacccagt ggctgtagat tccatacccg ctgctggttg
      121 gcagaagaaa gatgtagaag agaggaacct gaattcaagg aaattaaaga cggtcattat
      181 gtggcctgtc atgtaatgga agaaagatat agtgactgat aataaaatac tggggaggtc
      241 tattattgaa aaccaaaaaa atacaaaatg agattaaaaa aatagaagaa gacatagtta
      301 aattaagaca ccttcttcat caatatcccg agattggtta tgaggaatat aatacagctc
      361 aattaatcgc taagacctta aaaaaattag atttagaagt taatgatgat atttatgcca
      421 ccggtgtgtc tggcctactt aaaggaaaag aaaacaataa aacaatatta ttaagggcag
      481 atatggatgc tttaactata caagagaaga cagatctgcc ttatgcctcc caaaaagatg
      541 gaataatgca cgcctgtggt catgatgtac actctgccat tgtattaggg gcggcggtgg
      601 tcttaaagga attagaagaa gaattaccgg gtaatgtaaa gttcgtcttc cagccagccg
      661 aagaacagga aggcggagcc ctgggaatga ttgaaaatgg agtaatgaaa aatcccaagg
      721 tagatgccgc cttaggatta catgtctggg gttcagccaa aaaaggtaca gttgaaataa
      781 aatatgattc aatgatggct tcccccgata ggttcttaat taagatcatc ggagagggcg
      841 gccatgcagc caatccccac caatgtgtgg atcccattat tatagcaact gagatcgtgc
      901 aagaatttca aaatatcatc agccggaagg ttgaaccaac tgaaccggct gttttatcgg
      961 tttgtcatat agaaggcggg aatacccata atgtaatacc ggatgaagta attattgagg
     1021 ggacagtaag gacccttaca gaagatgtaa gagacagcat accctccctc atggaaaaga
     1081 tgcttacaaa aattactgaa atttatggag cagaatatga attagattat gattacagct
     1141 ttccacccct ggtaaataat aaagagatga ctgaccttgt tagaaattct gctaataaag
     1201 taatgggaga agataaagta atagaaataa ataagccgaa tttaggtgga gaggattttt
     1261 catactttgc taaagaagta ccttcttcat atttttatct cggtattgcc ccttccaaag
     1321 aaaatgtaat aaaacatcat aatccctact tttatgtgga tgatgatgta attgcagatg
     1381 ggatagcaat tttagttcaa gctgtttttg attattttga taattgatat cttgcaacat
     1441 gttaaccaat ttgatattat gtcattaata ataaatagtg agatttgtag gtgaagaata
     1501 tgaaagtggc agttattgat ggattaggtg gaggcttagg tgctcaaata gttgaaaagt
     1561 tgaacaataa attgagcaac aaaattgata ttttagccct cggcacgaat gccgtagcta
     1621 cctccaagat gctagagcaa ggcgcagatg aaggtgcaac cggtgagaat gctataaaat
     1681 tgaatgttaa caaggttgac cttattatcg gtcccctggg acttattata cctaactcca
     1741 tgaaagggga aataactacc gtaatggccg aagccatagc cggtagtgat gctaaaaaga
     1801 taattcttgg aatgaggcaa cctcatgtag atttggttgg tttaagtgat aagaccttaa
     1861 acgaacttat tgatgattct atcaaaaaga ctgaagaatt tttaaaagga aagtaagatt
     1921 aattacaaaa aaatattttt aatgatcacg aaggtcataa gctgtctaat gcggacaggg
     1981 ccttcgtttt ttctattttt aaagggggaa ttaaattgga tattaaaaag attgtttata
     2041 cggcattttt cattgcttta ggtattgtct taccgatggt atttcattta gtggggggac
     2101 cttctttagg tagagttttc ttgcccatgc atttacctgt cttaatcggg ggagcctttc
     2161 taggccccat ggctggacta ataattggta ttattactcc cttattaagt tcattgttta
     2221 caggtatgcc gccggtaata ccgatgctgc ctattatgat agctgaatta ggtatatatg
     2281 ggctggttat gggatatttc ttctttagaa tcaagatgaa tgtatatctt tcgttaatta
     2341 tttcaatgct tgctggcaga ataatggctt cccttgtggt attagcatta gtatatgggt
     2401 ttggttttgc ccgcttacct gctaatccta taatatatat atatgggact ataacagccg
     2461 gttttccggg tattatcggc cagcttttaa tagtacccct tgtactgagg tatattaaaa
     2521 gatttaacga acaaaaacct actttagaaa cagcctatta aaaatataat aacagtcaac
     2581 aaggaattag cctgtcttct atctaattaa taatacaaga gataaaatat agaagacagg
     2641 aggtagctta ggatgcccgc taatttaccg cctcagtatt atgaagcaga agaagtatac
     2701 agaaatgctc aaacaacgga agaaaagatc gctgccttaa gagagatgct ctctatcatg
     2761 cccaaacaca agggtacaga aaaactgcag gcagatttaa aaaagaggat ttccaaagta
     2821 aaagaagaaa agaaaaagaa aagtaaatca aaaagcacct ataaccccta tttaatagat
     2881 aaagaaggag ccggtcaggt aattttattg ggttcaccta atgttggtaa atcttcgatt
     2941 ttggattctt taagtaatgc caaggtggaa gttgctgaat atccttttac cacttcttta
     3001 cctttggccg gtatggttga atatgaagat attcaaattc aattaattga tacaccgcct
     3061 ttggtgccgg aagatgttcc cggcccgatg attagtgcaa tatttcatgc tgattatata
     3121 acaattgtta ttgatgcttc agctgatgat tgcttggatc aactatcagg tatcatcgaa
     3181 tttttagaag ggaaaagaat aattagagat gaaataccgg agaatgtaaa gggctaccat
     3241 ttagaagata ttttagtatt ggccacaaaa gtagatcttt caggttccaa ggataatatt
     3301 gaaataataa aagaactata tcccaatctg gatctattaa aaatatcgaa taaaacagat
     3361 caaaacttag ataaactacc aaaaattctt tttaataagt taaatgtaat aagggtttat
     3421 tcaaaagcac ctggggaaga acctgattat gatagaccct atatcttaga taaaggtgaa
     3481 acagttcttg attttgccca gcttgtccat aaagattttg caaagaactt acaaaaagcc
     3541 cgaatttggg gttcagccaa atttgatggc caacatgtag caaaagatca tgttttagaa
     3601 gaccaggatg taattgaact tcatacttaa tagtaaaaag aaaggaagtt tattagtaat
     3661 ggaagaaaaa caactagcta ttaacgaact taataataca gattattcct tagtagttgt
     3721 taaaaatagt aaagttgaat atagaagcaa agaagaaagt gttggttcta tagttaacct
     3781 cttagagaac aggccagaac tgcttaaaaa cgcagttgta gcagataaaa taatcggtag
     3841 ggctgttgct atggtatgtg actttgcctc tgtaaactac tgttacgggc agatagttag
     3901 caaaggagcg gtagatgttt ttgaaaacaa caatctatct tataaagcag ccgaagtagt
     3961 ggaagctatt aaaaatagag ataatacaga cttatgtccc attgaaaaac ttaccttaga
     4021 tataaatgac agtgctaaag gcattgaaaa aataaaggag tttcttaata attaaattaa
     4081 gtgggtgaag caaatgggaa gttatattaa gatctatatt ttggcattta ttatttttat
     4141 gggaattgac ttagtttggt tgggtatttt agcgaaggat ttttatagga gtcagctcgg
     4201 taccttttta aaagagagct ataatatgaa agcggcccta ttattttatt tagtttatat
     4261 tgcaggacta atattctttg tagtgagcag agctattgct actgggaatt ggcagtatgc
     4321 tgtttttgcc ggtatgtttt atggttttgt tacttattca acctatggaa ttaccaatta
     4381 ttcgacaatt aaggattggc caacattatt aactattatt gatattggat ggggaactgt
     4441 tctctgtgga ctgacctcat atttaactta tttaattgcc agtaatttta gtttttttct
     4501 taattaaagt aataattatt aatatataaa ctaatatctt aaattaaaga taataaatga
     4561 taagctttga gcctctaata tatggggctt tttttatttt caaacaaaaa aatataaaaa
     4621 atccttgcat tataatacga tattatgtat aattatttaa aattaattaa gtaaatctgt
     4681 ggaacgggag agtagaagaa ataactgagt aagcgagatg gggttggtgt aagcccattc
     4741 ggttattact ttgaaccgca cccgggagga gctgttctga ataaaaagta ggaacagacg
     4801 tatgattaac gttaaaaatt ataagagtgg tcaaagtttg tagacaaatc agagtggtac
     4861 cgcggataaa agtccgtctc tactaaagaa attattagta gggatgggct tttttatttt
     4921 aaagaagggg tagataatat gaaaaaattt acacgcctcc gaactagata aaataatata
     4981 ttaatcagag gacaatcata taatatttaa accaaaatat aaagggaggc ataaaaatgt
     5041 ctaaaaataa taaaaagatt gaaaaaatat tacttgctta ttcaggtggt ttagatactt
     5101 ctgtatcaat taaatggtta aaggaaaaat ttgcggctga agtgattact tatacggctg
     5161 atctaggtca aaaacaggac tggaaaaagg taaaagaaaa agccttagcc acaggggcta
     5221 gtaaagcata tatcgataac cttgaaaaag aatttgttga taattatgta ataaaagggc
     5281 taaaagcaaa tgcactttat gaaaataaat atcccctggc aactgcttta ggtagaccct
     5341 taattgtaga aaaaatggtt gatattgcta aaaaagagaa tgtcgatgct ttagcccacg
     5401 gctgcactgg taaaggaaat gaccaggtta ggtttgaagt gggttttaaa gccttagcac
     5461 ctgaacttga agttattgct cctttaagag actgggagtt taaaagtaga gaagaagaga
     5521 tagaatatgc taaagaaaac caaataccgg tcgatgctac caaaagcagt ccttatagta
     5581 tcgatcaaaa tctctgggga gttagtattg aatgcggctt attagaagat ccctggaatg
     5641 aaccaccggc cgatgcctat ttatggacta aagatcccca tgaatctcct gataatgagt
     5701 tgaaactaga aatagagttt aaaaaaggta aacctatttc acttaatgga actataatgg
     5761 acactaaaga tattattaaa aaattgaatg aaataggtgg tcagtatggg attggtagaa
     5821 tagatctagt cgaaaacaga ttggtcggaa ttaaatctca tgaaatttat gaaacgcctg
     5881 ctgctgaaat tctacttaaa gcacatatag ctttagaaga actaaccctg gataaagaaa
     5941 ccaagcagtt taaaaatatg attgagaaca agtatgccag tctaacatat aatggtctct
     6001 ggttctcccc cctaaaaaag catcttgata aatttattga agaaactcag caaaatgttt
     6061 ccgggattat taaattgaaa ctatataagg ggagttgtac cgtcattgga aggaagtcgc
     6121 cctactcatt atataattat ggcttagcta cttatgacca aaatgaccaa tttgaccaca
     6181 actcagcgca gggttttata aatctatggg ctttaccgat tcagattgct aataatgtcg
     6241 attcttatat cgataaaaat agtaaggagg cagttaaatg aaactttggg ggggaagatt
     6301 tgataaaaac acaactttaa gaatggataa atttaatagt tcattacctt ttgatatcag
     6361 attattacct tatgatattc aaggtagtat agttcatgta aagatgttag ccaagcaaaa
     6421 tataataaaa ggggaagaaa aagaagcaat aataaagggt ttaaaggaaa ttgaagctga
     6481 attagaagag gacatttcaa ataataaatt tgattattat gaagcagaag atatacattc
     6541 tttaattgaa aagcggttga ccgggaagat agggtcaacc gctggtaaaa tgcataccgg
     6601 tcggagtaga aatgatcagg taaacctgga cttgaaacta tatttaaacg aaataactga
     6661 aaaaataaaa gagcttttac ttaacttaat ggaaatactg gttgatatgg cagagaataa
     6721 ccatgatgta ataatgccgg gctatactca cctccagagg gctcaaccta taacttttgg
     6781 acatcatatt ttggcctatt tctacaagtt taaaagagat ttggaaagat tagaggatta
     6841 tactacaaga ttaaacattt cacctttggg ttcgggggca ctggcgggca gtaatttcaa
     6901 tttagaccag gaatttacgg ctgaacagct tgggtttgca gggatatatg aaaattcaat
     6961 tgatggagta agtgataggg attatgtctt agaatttgag tatattgcag ctaatataat
     7021 gattcatttt agtaatataa gtgaagagat tataatctgg aatacttcag aattttcttt
     7081 tattgaacta agtgatgagt atacaaccgg tagttcaatt atgccccaaa agaaaaatcc
     7141 cgatttggct gaactaacca gaggaaagag tggaagggtt ttcggcaact taatgcagat
     7201 gttaacagtt atgaaaggat tacctttggc ttataataaa gatctccaag aagataagga
     7261 agggcttttt gatacctgtg atactatatt aagaatatta gaagtctatc cagatatgtt
     7321 aaagactatg agaataaata aagagaagtt aaaggaagtt gttgaaaatg attatttaat
     7381 tgctacggat ttagctgatt atctgacaag taaagatatt ccttttagaa aagcacatga
     7441 acttgtaggg gaaatagtta agtatgcaga ggacaataaa aagaatttaa aacaacttaa
     7501 gtttgctgag tacaataata tacttggaca gtatcatgag tatatagata aagatatata
     7561 taaattattt gacttagaag aatcgataag cagtaaaaaa acagctggag gcccttcaaa
     7621 taaaagggtg aaggaacaaa ttagacaagc tagaaacttt attaatatca aatgatgaag
     7681 aaccgatttt aaaaaattta aaaaatttag atataaataa aggattttta gttataatct
     7741 ttaataatat taattgtgtt gccaaaattt tattgggagg tgaactttat ttggatgaga
     7801 tacgggatga attaataaaa tgcggcacta cactcttttc aaaaaaagga tatagggata
     7861 ccaaggtaaa agatgtcaca gatttggcat caatcagtgt tggtactttt tataattatt
     7921 ttgattccaa agaagaacta tttctagatg tatacttgcg aaaacataat cgagccaaac
     7981 aagaactttt agattttttc aaagaagata aaccacctct caagataatt gataaagcag
     8041 tacataggtt tttagatatg atgcagacgg atcctatttt aagagctttc tttaatccaa
     8101 atatacatga aaaatatcgt cagagcctcg atatcaaaag gaaaaagacc catttaaatt
     8161 atgcttatga tctatttgct cctcagctgg aaaaatggca ggacagggga gagattaatg
     8221 aagaagtaga tatcaaatta ttattggccg tttttgactc tattttttat gttctattac
     8281 ataagaaaga tataggtgaa gaatttttcc cagagatagt cgatttttta attgatagtg
     8341 tattgtgtaa aatgaacaaa aatttttacc aatagatgaa tgaataattt tattattcat
     8401 tctaatcaga tgtttagaaa gtttgcaata tccttttttt acgttacact aatatctttc
     8461 aaattggagt taataagaat ttaacacact tgatttaata acttagatgt gataatattt
     8521 tattgttaaa atattaaaaa ttttttaaaa aagcgtgaat gaataaagca tatattcatt
     8581 tcattaaatt taaaaaatta ctaatatttt taatatttca gacaatttta ttgatttcaa
     8641 gaactttttt tgataactta tatttgttaa agtataaaaa tacatttttt tatagaataa
     8701 tttatataac aataaatcac ttaaaattga gaatattaaa agagggggtg ttctcaaaaa
     8761 aaataagtag tatttaagta gattagagta atatgtattt taaaaatacg gaggaggaaa
     8821 aattttaaat gaaaagatct agtattctga ttttagtttt aagtatgttt tttgttttat
     8881 caatgtcaat aggtgctttt gcacaggtaa ataaccctac tacttttacc aaggtaaata
     8941 tgggtactat tgacagttta gatcctcact atcaatatga caatgctagt gcagaaattt
     9001 atgctaacat ctatgagaac ctgattatgt ttgacgaagg tgatgttact cagtataagc
     9061 ctatgattgc aactgaagta cctaccgtta ataatggttt agttaaagat aatggtacta
     9121 cttatatctt cccaattaga gaaggagtta cattccacga aggcggtaca ttaactgcag
     9181 aagatgttaa gtatagtttc atgagaggtt taatacacga ccgtaatggt ggtccttggt
     9241 ggatgcttta tggtcccctc tttgatgcag gtggattatc tgatattaca aacgaagttg
     9301 taggagtaga agatccaagt caacttacag cagaacagtc cgcagaagtt tttgaagtat
     9361 tggataaagc aattgaagta gatgaaaaca atgttatctt ccacctacct aaacctaatc
     9421 ctactttcat gagtgttatt gtacatgata atggtttggg cgcaattatg gataaagaat
     9481 ggtgtattga acagggtgcc tgggatggaa gccctgaaac aatcgcagaa tattatgacc
     9541 ctgctaaaga agaagatcca cttcacgaaa acactaatgg tacgggacca tttatgctaa
     9601 atacttggga aaaaggtaaa agaattgctt tagatagatt tgacggctac tggagagaac
     9661 ccgctcaact tcaaagagtt atcattaact atattgatga atggtcaact cgtaaattga
     9721 tgctgcagcg tggtgacgct gatgtagttt acatggacaa acagtatcaa agtcaagttg
     9781 aagacttaga aggtgtagaa ttaattactg gcctacctaa cctctataca ggttatggac
     9841 taatgaactg gacaattgaa acaaaaggta atccagatgt acacagtggt cagttagatg
     9901 gtaaaggtat accttctgat ttctttgctg acatccatgt aagaaaagca tttaactact
     9961 caatgaatta tgaagcattt gttcaagatg taatggatgg agacggccag caagcacgcg
    10021 gtcctattcc agagcctttc ttaggctgga gtgaagattc ttctatctat accacagacc
    10081 ttgccaaagc tgaagaagaa tttaaaaagg cttttgacgg agaattgtgg gaaaaaggat
    10141 ttgaaatcac aatattatat aactcaggta atagtatgcg taaaactgct acagatatgt
    10201 tgaaatataa tattgaacaa atcaattcta aatttaaagt aaatacccgt ggtattcagt
    10261 gggctactta cttagacaga atgattgctg gtaagtttac tctcggtttc attggatggg
    10321 gagcagacta tgctgaccct cataacttta cagttccctt tatgagaact gatggtacat
    10381 ttggtaactt taaaggtgaa aattacaaga catgggcaga agaaaataat gttgacgaat
    10441 tagtcaataa aggtgtggaa gttacagagc gtgcagaacg tctagagatt tataaagaat
    10501 tacaacatat gacttatgaa tatgcaactg acatgtatct atatcagcca actgctcata
    10561 ttctaaaaag agaatgggta aaaggttggt actatgatcc aattctacat gatggtattt
    10621 attactacag tattcacaaa gctgcagagt aattaaattt aacaaagtaa aatgattaaa
    10681 aataatatca ggaagggaat attcccttcc tgatatctat tattttataa aagaaggaga
    10741 tttgaaatga tatcatatat tgtaagaaga atactgatac ttccaataat tctaattgga
    10801 gttacattat tagttttcag tatgattatg atgcttagtc cttatcaaag acttagtact
    10861 tacataaaag atcccgccca attaaaaagt gtagatgtag atgtcttagt agccagacat
    10921 ggacttgatg atccctggta tcagcagtat gggcgctgga tttcaggagt agcaagaggt
    10981 gatttggggt ggtcagaatc agcaggtgca acggtctcgg aagccttttt gtctaggttt
    11041 ccagctacag cagaattagg attatttgct ttattaccgg tggtattagg aggtattatt
    11101 ttaggagttt ttgcatctgt tcatcataat gatttttggg atcacgtgag tagagtatta
    11161 gcgataattg gatggtcggt tccaacattt gtttttggtt tagtaatgtt gatgatattt
    11221 tacggacatt tgggttggtt cccaccttca aggctcgata gctgggcttt agaaatagtg
    11281 aattcttcag gcttcataaa ttatactaat ttctttgtgt tagatggatt tttaaatggt
    11341 aatttagcta ttgtcggtaa tgctataaga cacttgatcg cacctgtagt tactatctcc
    11401 attttatggt gggcatttat tatgagaatt acccgttcta gtatgcttga gattttgaga
    11461 aaagactata ttagaactgc ccgcgctaag ggtgttacag aaaatgttgt tgtatataag
    11521 catgctgtga gaaatgcttt aattcctgtt gtaacagttg caggtagaat ggtattaggt
    11581 ttactcggtg gattagtcat tacagaaact atattcaata ttgaaggtct cggccaattt
    11641 atggctcagg cagcacaaca gcttgattac ccagctgttt taggaggaac cctgtatttt
    11701 ggttttctct taattatgat taacctttgt gtcgatgttt cttatgctgt cattgaccct
    11761 agaattagat tggagtgatg taattggaat ggcaagaagt aactcttagg ctatttaaaa
    11821 ataaaatttc gttgataggt ttaattttag taataggatt tggattaatt gcagtatttg
    11881 ctccctggat agcacctcca gctgatccga gcgaaccata catggttcca agagcaggtt
    11941 ggggttttga acctaagcct cctagtgcag aacatatttt tgggacagca gaaggacaat
    12001 atgatatatt ttatggtata atatggggta caagaactgc atttagaatt ggttttatta
    12061 ttgtagggat tagtacttta attggaatag caattggaac cctggctgga ttttatggag
    12121 gctggtttga tgaaatagta atgagggtga cagatatttt catggctata ccatttttaa
    12181 ttgccgccat cgttttaaca acaatcctgg gtcagggatt acggaatgtg atgattgctc
    12241 ttatcacctt tggctggatg aatacggccc gtctaatgcg ttcacaggta ttatccatta
    12301 aagaagaaga atttgtaatg gctgctgatg ccttgggtgc cggtgatgtt aggataatat
    12361 tcaaacatgt aattttaaat actattttcc ctttagtaat tgaagcttca atgagaatgg
    12421 gttcaatggt tattactgct tcaaccttaa gtttcttagg aatcggagca ccagaaggtt
    12481 atgctgattg gggacagatg atttcatttg cacgtaactg gattttagga ggtcaaggga
    12541 acgctattca atactggttt acagttgtaa ttccgggttc agcaataacc ctattctgct
    12601 tgtcctggaa tttaattgga gatgcattcc gtgacatctt agatcctaaa ctacaataaa
    12661 aagaagtagg tgaatttagt tggcggaaaa taaacttatt gatgttaata atttaaaaac
    12721 ttacttccat actgaagaag gaaccgtgaa agcggttgat ggagtaagct tcgaaattta
    12781 tcctggtgaa actctcggag ttgtaggtga gtccggctgt ggtaagagtg ttacctcact
    12841 ctctatcatg agacttatcg aatcaccacc tggaaaaata gaagaaggcg aaattttatt
    12901 taatggtaaa gatttaacta aattaagtca gggccagatg agaaagatca ggggtaatga
    12961 tatctcaatg atcttccagg agccaatgac atccttaaac ccggtttata ctgtgggaga
    13021 tcagattatg gaggcaattc tcattcataa aaacgtgaat aaaaaacagg cccgagaaga
    13081 agccataaac atgttaagaa aggttggtat acctctacct gagcagcgtg tcgatgaata
    13141 tccccaccag ttaagtggag gtatgagaca gaggataatg atcgcgatgg ctttatcctg
    13201 tgaccctcaa ttattaattg ccgatgagcc taccactgcc ctggatgtta caattcaggc
    13261 tcagatatta gagctaatga atagtttgaa agctcagttt ggaatggcga ttatgatgat
    13321 cactcacgat ctgggtgtta ttgctgaggt atctgataga gttgcagtta tgtatgccgg
    13381 taaaattgtg gagtatacag atgttaaaac tttatatgcg gatcctaaac atccttatac
    13441 ctggggttta atgcattcta tacctcatat tgagaaagac ttagatagat tagaggcaat
    13501 ccccggtaat gtaccgaatc cattggactt tccggacggc tgtaagtata atacaagatg
    13561 tccttttgca accgataaat gtcgagtcga cgaacctgat attgaagagg tagaagaagg
    13621 tcattattca gcctgctggc atgtcgataa attagaagct aaaaaatcag aagagaaagg
    13681 cgcttaatgg aggtgaaaag atttggcagt agaacaagaa agattacttg aaataaaaaa
    13741 cttaagaaag tacttcccgg tcaaagaagg agtatttcgt aaaaccgtcg cccatgtaaa
    13801 agcggtcgat gatataagtt tttatatcaa aaagggagag accctgggat tagttggtga
    13861 gtccggctgt ggtaagtcta cagcaggccg tacaatcctt cgcctcctgg aagcgacaga
    13921 aggagaaatt tggtttgaag gcaagaattt attatcttta gataaaaaag aactaaggga
    13981 aatgagaaaa gagatgcaga ttatctttca ggacccttat gcctcattaa atccccggat
    14041 gacagttgcc gatatagtgg gggaaccaat agatattcat aatcttgcct catctaaaaa
    14101 agaacgtaat gaaaaggtag cagatatact tgatacagta ggtttaggtc ccgaatatat
    14161 gaaaagatat ccacacgagt tcagtggtgg tcagagacag cgtatcggtg ttgcaagagc
    14221 cttagcggtt gatcctacct tgataattgc ggatgagccg gtttctgcgc ttgatgtttc
    14281 cattcaggct caggttataa acttactgca ggacttacag aaagactttg atttaactta
    14341 tctatttatc gcgcatgatt taagtgttgt taaacatata agtgatagag tagcggtaat
    14401 gtaccttggt aaattagttg aattggcaga taagaaggaa ttgtttgatg acccgaaaca
    14461 cccttatacc cagtctctgc tctcagcgat tccggtagca gatcctactt atgagaagga
    14521 gagaataatc ctggaaggag atgtaccttc accggtagat ccaccatctg gttgtaggtt
    14581 ccatccccgc tgtccatatg caatggatgt atgtagtgaa aaagaaccag agttcaaaga
    14641 ttacggcgat gaacattttg ctgcctgtca tcttcttgaa caggaataaa taaaatataa
    14701 ttgttttact caattctatc gtgcttacag ggagaagtca cctaacacaa aaggtgactt
    14761 ctaccccaag agtaaaaatt ataagttaag atggaagctc aaatatatgg gttttcatct
    14821 ttttttgttt ctttaaattt tgcactatta cctaaaatat agtaaaatat gattaagtca
    14881 atcaaaataa taataaaaag gagttaatta atgaaagaaa aaattgctgt tgtaactgat
    14941 agcacgtgtg atttgtcgag tgaaatatta gaagaacaag aaattgagtt tttaccttta
    15001 aaaataatat atgaaaataa ccaatataaa gatagagttg atattaaacc tcaaactgta
    15061 tatgacaatt ttgaagatga aataccttct acttcaatgc cgactccttc cgaagtcaga
    15121 gaaaaatatt tggaactaca tgaaaaagga tatacccata ttatttcaat ccatatttca
    15181 tcgggtttaa gcggaactta taatatttgt cagatggttg ctgatcagtt agatgaaata
    15241 acggttcaag ttattgattc caaaatgtta tctatgggtt tgggaagatt agttttatat
    15301 acaaaggagt taattgattc taatcaacat agctttaatg aaatagttga taaagtagag
    15361 accaaaaaag aagaaatcga tttatatttt atcgtagata ctttaaagta tctcaagaag
    15421 ggcggcagaa taggtaaggt gagaggtaca attgctgaat tactaaacat caaacctatt
    15481 atttcaatta atgaagaagg tgaatatttt acatttgata aagttcgttc ccggaaaaaa
    15541 tcaattacaa agttaattaa tttagcgaaa gaaaagataa atgagggctt ttgttatgtg
    15601 gatgttatgc atgctgatgc taaggaagaa gctgtagata tattagataa aatgaaagat
    15661 ttaccaaatg ttaaagatac tgttttagga gaaataagtc cagccatggt agtacattca
    15721 ggtccgggac tgattggtgt ttgtataaca aaaatataat acagcctaga tagcatatta
    15781 taacaaaaat ttataataat ccataaacca gctagacaac taatacttgt ttggctggtt
    15841 ttttgatttt aatagtgata tctaattgga atatttgtat taaatactgg ggaaaaggag
    15901 agataaaaat gacttcatat aatcatctga aaggtgaaga aagtccctat cttaaacaac
    15961 atatgactaa tccggtggac tggtacccct ggagaaaaaa ggcatttaaa aaggctaaga
    16021 agaaagatct accgattttt ttatctatcg gttattctac ttgtcactgg tgtcatgtga
    16081 tggaagaaga atcctttaca gatcaagaga tagccgaaat tttaaatgaa aactttgtat
    16141 caataaaagt agatagggaa gaaagaccgg agattgataa attatatatg gaagtttgta
    16201 aagtgatgac gggaagtggg ggttggcctt taagtatatt tatgacccct gataaaaaac
    16261 cattctatgc ggcaacttat attcctaaaa atgataaata tggaagaaga ggtttactat
    16321 ccatacttcc tcaaataaaa aaattatggg acagccagag agaaaaatta ctaagtacag
    16381 ctaatgaggt ggttaatcat ctaaataaaa atgaaagttt tgataaagtt gatattgatg
    16441 aaagagcaat tgaaaaggca gccggtatat tggacaggat atatgataaa aaatatggtg
    16501 ggtttggtaa ttctcctaaa tttcccatgc cccattattt gctttttcta ttacattatg
    16561 cgaaaaacag caataatgat aaatatctga acatggtaga aacaacttta cagaatatga
    16621 gggctggggg aatttatgat caactcggac agggatttca ccgctattca actgatcaaa
    16681 aatgggaggt ccctcatttt gaaaaaatgt tatatgacca ggccctttta atttatactt
    16741 accttgaaac ttatcgagag accaataaaa atttttatag agatatagta gaagagataa
    16801 tagattattt aaaaagagac atgctgtccg aaacaggcgg tttttatgca gctgaagatg
    16861 cagatagtga tggtgaagaa ggtaaatact attattggaa tctatcagag ttagaaaata
    16921 ttttaactaa agaagaatat gataaatttt tatctgtatt tactattgat gatgaaaaga
    16981 aaataaactt gagattaaaa aatgttaaag attataaaag tattacaaac attaaacaaa
    17041 agttattaga tgaaagagaa aagagaacga gacctggaag agataataaa atattaaccg
    17101 attggaacgg tttaaccgtt gcagctttag ctaaagctgg ttttgtgctt aataaagaag
    17161 agtatattaa actggcagaa aaaactttaa gttttataga taaaaaaatg aagtcggata
    17221 aaggaaaact ggctcatagt tattgtaatg gtaaggtaac ggaatatagt aatttagatg
    17281 attatagttt ttatctatgg ggtttaattg agctttatca ggctacttta aaagaaattt
    17341 atatcaataa agcagtagaa atagcagaag agatgataaa actattttgg gatgaaaaag
    17401 ggggaggttt ttattatacc actaaggaaa atcaagaatt atttttaagg caaaaagata
    17461 cagctgatag tgccatcccg gctggtaatt cattagctgg atataatttg ctgagactag
    17521 ctcaactaac tgataatatg cggtatagaa gtataactga aactttgatc gaggccctat
    17581 cgaaagatat ctataataat cctgtcaacc acatattttt attattttct tataaatatt
    17641 tgcaagagcc ttttaaagaa ataaaaatta ttggcgattt aaatgaggaa aaagcagtaa
    17701 atttaatgaa taaaataaga aataattatt ggccggaact gttaattaaa catgagcaga
    17761 aagaagaaaa gataatgttt tctttgtgta aagattttgc ctgtaagcca cctactgaag
    17821 atatagaaga gatattaaga gaactataaa gtgatcttat gcaattagta ttgtttgtat
    17881 tacaaaaaat atttattaaa aatgtaaata attaaagagg aaatataata ataattttga
    17941 atattataac tagaatatgt tttaaaagag gtagcaaaat gcttttaata aaaaatgcta
    18001 atttggtcga taaagagggt tatattgata ttttaatcca ggacggtgtg tttaaaaggc
    18061 ga
//
LOCUS       DATGCJ010000058         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_51492, whole genome shotgun sequence.
ACCESSION   DATGCJ010000058 DATGCJ010000000
VERSION     DATGCJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51492"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VKN64_02900"
     CDS             complement(<1..657)
                     /locus_tag="VKN64_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMA59215.1"
                     /translation="MGRIQDFSVLRVDLENREIKREEIAGSHVEKYLGGRGLASKILY
                     DELDPKIDPLSPKNKLIYATGLLTGTAASTGGRYMVVTKGPLTGTIASSNSGGFFGAE
                     LRFSGNDLIIFEGKADKPVYLSIKGDQIELKDASHVWGKKVPETTDTLIEEFGDERAR
                     VSCIGPAGENEVLFASIMNEKNRAAGRTGVGAVMGSKNLKAVVVKASEKQVKYNKEEA
                     M"
     regulatory      complement(668..795)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(1016..1777)
                     /gene="mobB"
                     /locus_tag="VKN64_02905"
     CDS             complement(1016..1777)
                     /gene="mobB"
                     /locus_tag="VKN64_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HMA59216.1"
                     /translation="MKLFSVYGISLTGKTTTIENIIKELKKRRYSVGSVKEIHYEDFA
                     IDKEGTNTYRHSQAGAEPVTARGYNETDILYKERLSLQKILRNYSQDYVILEGVEKGN
                     FPKIITAKEREEIDERLDDMVFAISGKIADEIDEYKGIPVINCINETEKLVDLIEEKV
                     FEKLPDFPADCCTACGFNCCELGKKILRGEAKRSDCVISDTNTKLYINGKEIDMVPFV
                     QRLLYNAVQGVVSELDGYQEGSSIKVKIGNDKTCK"
     gene            complement(1783..>2413)
                     /locus_tag="VKN64_02910"
     CDS             complement(1783..>2413)
                     /locus_tag="VKN64_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HMA59217.1"
                     /translation="ISIIVSNFFKEDWGFLNRARKFIVLLVGIAFLQSIFSPSGDILI
                     SISNFTLLTTGGLVKGLRVILRMLIIIISATIMKGSSPREIIQGLIQWKVHYEISFMV
                     SLAIRFIPILGQEAKDAFTAIQLRGIEIDKLSLTKRFKVYSYMFMPVLSGVIHRAREL
                     STSLEARAFRAYPERTSYIKLELSKNDFLIMSLSIIFTISMLTIYILMG"
BASE COUNT          775 a          487 c          303 g          848 t
ORIGIN      
        1 cattgcttct tctttattat atttaacctg tttttcagag gccttgacta caactgcttt
       61 aaggttttta gaacccatta cagctcctac gccagttcta ccagctgctc tgtttttttc
      121 attcattatg gaagcaaaaa gtacttcgtt ttcaccagca ggacctatac aacttactct
      181 tgctctctca tctccaaact cctcgattaa agtatcagta gtttcgggta cttttttacc
      241 ccagacatgt gaggcatctt ttaactcaat ttgatctcct ttaattgata gatataccgg
      301 tttatctgcc ttaccttcaa aaattattag atcattaccg ctaaatctta actcagcgcc
      361 aaaaaaaccg cctgaattgc tgcttgcaat ggttccagtc aaaggccctt tggtaacaac
      421 catataacgg ccacctgtag aagcagcagt acctgttaat aaaccagtgg cataaatcaa
      481 tttgtttttc ggactcaaag gatcaatctt gggatctaat tcatcataga gtattttaga
      541 tgcaagacct ctcccaccta gatatttttc tacatgtgaa ccagcaattt cttctcgctt
      601 gatttctcta ttctctaaat ctactctgag cacagaaaaa tcctggatac gtcccatttt
      661 ttaattaccc ccttaaaatt tctctccttt ccgaacacgg gaggccaaag tatttctact
      721 ttaacccagc ggtcaattca ccataaaata ataggtttat ctcttaggtt caaagactcg
      781 gaggtatatt ataaatatta tacaaaaagt tgagtaatcc tgccagattt ataaaattaa
      841 tcgaatattt tttgttatca ggaaattata attctaaaaa cgaagttata ttaataatta
      901 taatttatat aaatatttaa ccatatataa aaaccctgaa aataagtcat gaaaataata
      961 acttacttca gggtttctta aggtctatta tttatttttt ttataaataa taatcctatt
     1021 tacaagtttt atcattgcct atctttactt taatagaaga accttcttga taaccatcta
     1081 gttcactcac aactccctgg acagcgttat ataataagcg ctggacaaaa ggaaccatat
     1141 ctatttcttt accattaata tataacttag tattggtatc agatatcaca cagtctgatc
     1201 tttttgcctc acctcttaat atttttttac ctaattcaca acagttaaat ccacaggctg
     1261 tacagcaatc agccggaaaa tcaggtaatt tttcaaaaac tttttcttca attaaatcta
     1321 ctagcttttc agtttcgttt atacaattaa tcaccgggat tcccttatat tcatctattt
     1381 catctgctat cttgccagag atagcaaata ccatatcatc tagtctttca tcgatttctt
     1441 ccctttcttt agccgtaata attttaggaa aattcccctt ttcgactcct tctaagatta
     1501 cataatcctg agagtaattt ctaagtattt tctgtaaaga taatctctct ttataaagta
     1561 tatctgtttc attataacca cgggcagtga caggttcagc ccccgcctga ctatgccgat
     1621 atgtattagt accttcctta tcgatagcaa aatcttcata atggatctct ttaactgatc
     1681 caacagaata tcgcctcttt tttaactctt ttataatatt ttcaattgtt gtcgttttac
     1741 cggtcagact aattccgtaa accgaaaata atttcatttc tttcacccca ttaatatgta
     1801 gatggttaac atggagattg taaaaataat acttaaagac attattagaa aatcattttt
     1861 agataactct aattttatat aacttgttct ctctggataa gctctaaaag ctctcgcctc
     1921 tagagaagtt gaaagttctc ttgccctatg gatcactccg gataaaacag gcataaacat
     1981 atatgaataa accttaaacc tttttgtcaa tgacaattta tcaatctcta tacccctcag
     2041 ttgaatagca gtaaaggcat cttttgcttc ctgtcctaag ataggaataa atctgattgc
     2101 taaagatacc ataaaagaaa tttcataatg gactttccac tgaattagac cctggattat
     2161 ttcacgcgga cttgatcctt tcattattgt agctgaaata ataataatca acatccttaa
     2221 tataactctt aaacctttaa ctagacctcc tgtagttaaa agagtaaaat tacttattga
     2281 tattaatata tcaccagatg gtgaaaatat gctttgtaaa aaagcaatac caaccaataa
     2341 tacgataaat ttacgggccc tatttaaaaa tccccagtct tctttaaaaa agttagatac
     2401 tataatagat att
//
LOCUS       DATGCJ010000059         7649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_229769, whole genome shotgun sequence.
ACCESSION   DATGCJ010000059 DATGCJ010000000
VERSION     DATGCJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229769"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VKN64_02915"
     CDS             <1..743
                     /locus_tag="VKN64_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA59218.1"
                     /translation="NAIEDVASGAEEQTAQVEETSENINKGNKSIQQSVNKINQVKDY
                     YKDVADTMDSLGESSNEIGEIVNLINDIAAQTNLLALNAAIEAARAGEAGRGFSVVAD
                     EIRELAEESANATEQISELINDIQDDVDSAVDKMDKTEEAVDQGVETIETSGKIFGKI
                     NDTSQNSNQAIKKLHESIEEMNKNSKQVEEAVEQIEEVSKSAASNAQEVAASSEEQIA
                     STEEIVNAAKELAEMANELSDNVNEFNL"
     regulatory      1373..1447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      1541..1618
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1703..2326
                     /locus_tag="VKN64_02920"
     CDS             1703..2326
                     /locus_tag="VKN64_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HMA59219.1"
                     /translation="MNNSHINLNEIKKDALKEVGNIGAGNAATAFSEVLDRKIEIDVP
                     SVEIIPLEDVSTITGSEEDHVAGVLFKVSGEAPGTILFVFPSSTIKYLIEVVMDKKIE
                     PEEMGEIEISLLKELGNILSGSYLKSIGDMTNLNMNQSVPGFSYDMAGAILSSTMISA
                     SSTGENVLLIETEFMDGEEKIEGYFFFIPQVESLSIILKSLGLSGDE"
     gene            2319..2798
                     /locus_tag="VKN64_02925"
     CDS             2319..2798
                     /locus_tag="VKN64_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HMA59220.1"
                     /translation="MNNDEIRIKMAEYTVSKPPHVLVTLGLGSCIGLALYDKYLKVGG
                     LIHFMLPYSNGKKNTAKYADSGIPFLIEEMVKIGARKRNIVAKIVGGAKMFNFGEENS
                     AMNIGKRNSEAAKEILKTKSIKIVGEDIGEDFGRTMRFYTESGKVKVTSYKREDLII"
     gene            2936..3292
                     /locus_tag="VKN64_02930"
     CDS             2936..3292
                     /locus_tag="VKN64_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA59221.1"
                     /translation="MMKNVLIVDDAAFMRLNLKNILKDDYEIVGEAENGKEAVEMYQE
                     HSPDIVTMDITMPVMDGIEAIKAILDIDPEADIVVCSAMGQQKIVIQAIELGAKDFIV
                     KPFKKDRVKEAIAKLA"
     gene            3310..3753
                     /gene="flgB"
                     /locus_tag="VKN64_02935"
     CDS             3310..3753
                     /gene="flgB"
                     /locus_tag="VKN64_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HMA59222.1"
                     /translation="MNSTINLLEMGLDGAHLRHKTLSNNIANVSTPKYKRKDVNFIEA
                     LKSNINKIKKEPGDSNSSSIRLAKTNSNHFNLSASNARLHYNGVNKFKINNYSNTSYR
                     NDQNNVDVDAEMAKLAKNNLYYNTLTQRVAGKFGLLQEVIQKGSE"
     gene            3756..4193
                     /gene="flgC"
                     /locus_tag="VKN64_02940"
     CDS             3756..4193
                     /gene="flgC"
                     /locus_tag="VKN64_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HMA59223.1"
                     /translation="MFSTVNVSASGLTAQRLRMDLIADNIANVNTTRTEDGIPYRRKL
                     AIFRERNSSFSNAMASQMSGNQSAGEGVEVAGIREDTKPFKTVYRPNHPDANQEGYVE
                     MPNINITSEMVDMISASRSYEANVTALNTTKRMALKALEIGRG"
     gene            4199..4501
                     /gene="fliE"
                     /locus_tag="VKN64_02945"
     CDS             4199..4501
                     /gene="fliE"
                     /locus_tag="VKN64_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HMA59224.1"
                     /translation="MNMINPIQNRLQLLNLNQVSDDKQTETSKDFQQLLQDNLNKVND
                     LQLNSDQLTEDFALGKTDNIHQVMIAGEKAETAMKLTLAVQNKVIDAYKEIMRLQV"
     gene            4555..6129
                     /gene="fliF"
                     /locus_tag="VKN64_02950"
     CDS             4555..6129
                     /gene="fliF"
                     /locus_tag="VKN64_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HMA59225.1"
                     /translation="MWDSLTDYIQNIKELWGNLDNKAKIMIGAGTVLMLVVFGYLIVS
                     GDSTVYTPLYTDVVQEDAGKIVNSLKEKNIPYQLTNGGTTIEVPKSQVHQARIDLASE
                     GLPHQGVVGFEIFDQSNFGTTDFERKVNYYRAIGGELSRSIRVMRGVDYAKVQITAPK
                     DSIYLDEERSASASVLIQLDSGYRMNSAQISSITNLVASSVQDMSPSNVTVIDTSGNL
                     LSAESNGNNMNSQISQENMEVQNNFEQGLEEDLKRLLTKVLGPDNFSVNVSAKLNFDQ
                     REVESKTYTPVADGEGIIRSEEVIEETNDSTTNGNGGAAGTESNIPEYQAEGAAVQTN
                     QSEKTNTVTNYEINEQVERRVYAGGEVERMTVSVMVDGDQDQALLNKIEESVQTAIGY
                     NPERGDQVSVNSFNFDNSLEQEIAQAKEAEASAERSRMYIYGGLILLIIIILSVIIFF
                     IRRTMSSSEAVQTVQRGQMVDYQAGEAEETKDYSKPTLSEEEKQKKQLTEELEDIVAD
                     QPEDVAKLLKNWLMED"
     gene            6156..7166
                     /gene="fliG"
                     /locus_tag="VKN64_02955"
     CDS             6156..7166
                     /gene="fliG"
                     /locus_tag="VKN64_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HMA59226.1"
                     /translation="MSGMSELSGKQKAAVLLISLGPDMSAKIFKHLDNEQIEEVTLEI
                     ANMQKIGTELKNKVLKEFYHLLQANDYISSGGIKYARQILSKAVGEKEADNILNRLTA
                     NLQVRPFDNIRKADPDQLLNFIQGEHPQTIALILSYIEPSQASQILSSLSEEVQSDVA
                     KRIALMDRTSPEIIKDVENVLEKKLSSVITNEYASAGGIDSIVDILNQVDRGTEKNIF
                     DELDEKDPDLSEEIKKKMFVFEDIVLLSDKAIQLVLREVETHDIALALKTSGEEVEEA
                     IFNNMSSRARDMLQEDMEYMGPVRIREVEDAQQRIVNVIRQLEESGEIIIARGGESEL
                     VV"
     gene            7159..>7649
                     /locus_tag="VKN64_02960"
     CDS             7159..>7649
                     /locus_tag="VKN64_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HMA59227.1"
                     /translation="MSKIIKASQIIGKYKLSNVHERDFEVKEETAADNKGNNKKNTEE
                     NQKEKAEAIIKEAENKAKKIIEEARKEEQKIDQIKKEAYQEGYEQGKKDGYQETSGKM
                     QEIINTLNQEIEELKTQYEKELNQLQPQLINIASQMASKIINHKIEETPEVINDIVED
                     VMQ"
BASE COUNT         2810 a         1187 c         1577 g         2075 t
ORIGIN      
        1 ggaatgctat tgaagatgtg gcttccgggg cagaagagca gaccgcccag gttgaggaaa
       61 catctgaaaa tattaataaa gggaataaat ctatccaaca gtcggtcaat aaaataaatc
      121 aggtaaaaga ttattataaa gatgttgctg ataccatgga ttccttaggt gaatcttcta
      181 atgaaatcgg agaaattgta aatcttatca acgatattgc agctcagacc aatctacttg
      241 ccttaaatgc agccattgaa gcagcccgag ccggtgaagc tggacgtggt ttcagtgtag
      301 tagccgatga aattagagaa ttagcagaag aatcagccaa tgccactgaa cagatttcag
      361 aattaattaa tgatatccag gatgatgttg attctgccgt cgataagatg gataaaactg
      421 aagaagctgt tgatcaagga gtagaaacaa ttgaaacttc tggcaagatt tttggaaaga
      481 taaatgatac atctcaaaat tcaaaccagg cgattaaaaa gttacatgaa tcaatagagg
      541 aaatgaataa aaatagtaaa caggttgagg aagcagtaga gcagatagaa gaagtaagta
      601 agagcgctgc cagtaatgcc caggaagtgg cagcctccag cgaagaacag attgcttcaa
      661 ccgaagaaat agtaaatgca gctaaagaat tagcagaaat ggcaaatgaa ctttcagata
      721 atgtaaatga atttaaccta taaattagca aggctatcct taaaacctgt ttagggatag
      781 cctttagtac ttttaattta atatattcta aaattacaat atttactatc aatttaatta
      841 ttttataaaa atctcaaatt tttaggagga gataaataac ttttataaaa atattataat
      901 agcggtacaa aaaaattaaa taattcatat taagaatata attttcctga tatgaccata
      961 atatacctta atttggattt gttacctaaa tgtaaatatt tttgatatta cttgacaata
     1021 taccatctat tagatattat ttcagtataa tatcatagtt cccattaatt tacttcagct
     1081 aatatttatt ataattagca atattaaaca tctattgtaa taatatataa atttatgtat
     1141 aaaaaatctt tcttgtgtat gagaaataaa tatttttttc atataaagaa gtaatagttc
     1201 ctaatatgac aataaattac ataaaattgg attaaaatac ttaaaataat ttaaaaagaa
     1261 tttcttgaca ttagttaaaa tattagttat tattttagag taagataaga tttaataaat
     1321 attaattatt tttcttttta ttaaccattt gaatataaaa tatatttatc taatttgaaa
     1381 agggcaaacc tatctaagat aggggcgcaa agccatgggc ctttttatag gctgccaggc
     1441 tgccgaatta gtctagtttt tggcgcctat ggcattttgc tataggtttt atttatttta
     1501 taaacaattt atcaagaatt tacatatgtt taacaagtct atttgaaaag ggcaaaacct
     1561 ctgtgaggag gcgacgcaaa gctgtgagtc tttgaaaaaa gacggtcagg ctgccaaatt
     1621 gatgattatt tttggtacct acagcttttg ctgtaggttt tttcttttta atagtaattc
     1681 aaatagatca ggaggaagtc aattgaataa ttctcatatt aatttaaatg aaatcaaaaa
     1741 agatgcctta aaagaagtag gtaatatagg agcaggtaat gctgctacgg cattttcaga
     1801 agttttggat agaaaaattg aaatagatgt tccttccgta gaaatcattc cactagaaga
     1861 tgtaagcaca ataacaggta gtgaagaaga tcacgttgca ggtgtattgt ttaaagtgag
     1921 cggtgaagct cccggcacaa tcctcttcgt ttttccctct tccacgatca aataccttat
     1981 agaagtagta atggacaaaa aaatagagcc tgaagagatg ggtgaaattg aaatctcttt
     2041 actaaaagaa ctaggtaata tattaagtgg ttcatattta aagtctattg gtgatatgac
     2101 caaccttaat atgaaccaat ccgtccctgg tttttcctat gatatggccg gagctatctt
     2161 gagttccact atgatctccg ctagcagcac cggtgaaaat gttcttctca ttgaaactga
     2221 atttatggat ggtgaagaaa aaattgaggg atatttcttc tttatacccc aggtggaatc
     2281 tctctccata atccttaaat cactggggtt gagtggagat gaataacgac gaaatcagga
     2341 taaagatggc tgaatataca gtctctaaac ctccacatgt tttagttaca ttaggtttgg
     2401 gttcctgtat cggtttggcc ctttatgata aatacttgaa agtaggtggg ttaatccatt
     2461 ttatgctccc ctatagcaat ggtaagaaaa ataccgctaa atatgcagat agcgggatcc
     2521 catttttaat agaagaaatg gttaaaattg gggctcgaaa aagaaacata gttgcaaaaa
     2581 tagtaggcgg ggctaagatg ttcaactttg gagaagaaaa ctcggccatg aatatcggta
     2641 agaggaattc agaagctgct aaagagatct taaaaactaa aagtattaag atagtagggg
     2701 aagatatcgg ggaagacttc ggtagaacga tgcgttttta taccgaaagt ggaaaagtaa
     2761 aggttacttc ctataagaga gaggacctga taatttagtc ctttttcgtt attaccctaa
     2821 agaatagcca taattattat taaaaaaaat aatatttaat aaaaatattt tgttgaatat
     2881 ataaattttt gtataaagat aagatttctt atataacaat atttaaggag gaaccatcat
     2941 gaaaaatgtg ctaattgtcg atgacgctgc ttttatgaga ctaaacctaa aaaacatatt
     3001 aaaggatgac tacgagatag tgggtgaggc agaaaatgga aaagaagcag tagagatgta
     3061 tcaggagcat agtcctgata ttgtaacaat ggatataacg atgccggtga tggatggaat
     3121 agaagccatc aaggcaattt tagatattga tcctgaggcc gatattgtag tctgttctgc
     3181 catggggcag caaaaaatag taattcaagc tatcgaatta ggggccaagg atttcattgt
     3241 aaagcccttt aaaaaagata gagtcaaaga agcaatagcc aaactggctt aaagaaaggg
     3301 aggatgaagt tgaattcaac gatcaatcta ttagaaatgg gtttagatgg tgctcacctg
     3361 cgtcacaaaa cattgtcaaa taatattgcg aatgttagta cacccaaata taagagaaaa
     3421 gatgttaact ttattgaagc cctaaaaagt aatattaata aaatcaaaaa agaacctgga
     3481 gatagtaatt ccagttcaat tcgtctcgca aaaacaaaca gtaatcattt caatctttca
     3541 gccagtaatg cccgtttaca ctataacggg gtgaataaat ttaagattaa taattattca
     3601 aataccagtt accgcaatga ccaaaataac gttgatgtgg atgctgagat ggcaaaactt
     3661 gctaaaaaca acctttatta taacacttta acccagcgag tagccggcaa gttcggtcta
     3721 ctgcaggaag taattcaaaa gggaagtgaa taaaaatgtt ttcaacagtt aatgttagtg
     3781 cttcaggttt aacagcccaa aggctgagaa tggacctcat agccgataat attgcaaatg
     3841 taaatacaac caggactgaa gatggtatcc cctaccgccg gaagctggcc atatttagag
     3901 aaagaaatag ttcgttctcc aatgccatgg cttcccaaat gtccggtaat cagagtgcag
     3961 gagaaggtgt agaagtggcc ggtatcagag aagatactaa accttttaaa actgtctata
     4021 gaccgaatca ccctgatgcc aatcaggaag gttatgttga aatgccaaat attaatatca
     4081 cttcagagat ggtcgacatg atttctgctt cccgttccta tgaagcaaat gtaacagctt
     4141 taaataccac caagagaatg gccttgaagg ccctagaaat aggcaggggg taaaatagat
     4201 gaatatgatt aatccaattc aaaatcgctt acagctttta aatcttaatc aggtaagtga
     4261 tgataaacaa accgaaacca gtaaagattt tcaacaactt ttacaggaca atctaaataa
     4321 agtaaatgac cttcagttga attcagacca gttaactgaa gattttgcgc ttggtaaaac
     4381 agataatatc caccaggtta tgattgccgg tgaaaaggct gagaccgcca tgaaactgac
     4441 tttagcagtt caaaataagg taatcgatgc ctataaagaa atcatgagac tgcaggtttg
     4501 atgttaatga caacttttac acacttttta ggtgtttggg agagataata agacatgtgg
     4561 gattctttaa cagattatat acaaaacata aaagagctct ggggtaacct ggataataag
     4621 gctaagatca tgattggtgc aggtactgtt ctcatgctgg tcgtatttgg ttatcttatt
     4681 gtatctggtg attcaaccgt ctataccccg ctttatacag atgtggttca ggaagatgcc
     4741 ggcaagatag taaactctct taaggagaaa aatatacctt atcaattaac caatggagga
     4801 actactattg aagtaccgaa aagccaggtt catcaggcca ggatcgattt agcttcagaa
     4861 ggtctacctc atcagggtgt tgtcggtttt gaaatttttg accagagtaa ctttggaaca
     4921 accgactttg aaagaaaggt aaattactac cgcgctatcg gtggtgagtt gagtagatca
     4981 ataagagtga tgaggggcgt agattatgca aaagtacaga tcactgcccc gaaagatagt
     5041 atctatctgg atgaagagag atctgcttct gcttctgttt tgattcagtt agattctggt
     5101 tataggatga attctgctca gatcagttca attaccaacc tggtagcaag tagtgtacag
     5161 gatatgtcac cttctaatgt aaccgttatt gataccagcg gcaacttgct cagtgctgaa
     5221 agcaatggca ataacatgaa cagccagatc agtcaggaaa atatggaggt tcaaaataac
     5281 tttgagcagg ggttagaaga agatttaaaa agactgctga ccaaagtttt aggaccggac
     5341 aacttctctg taaatgtaag tgctaaattg aactttgacc agagagaagt tgagagcaag
     5401 acctatactc ccgtagcgga cggagaaggt atcataagaa gtgaagaagt cattgaagag
     5461 accaacgact ctaccactaa tggtaacggc ggggcagccg gtactgagtc taatataccg
     5521 gagtatcagg cagaaggtgc agccgtccag accaatcagt ctgaaaaaac aaatacggta
     5581 accaactatg agataaatga acaggtagaa agacgcgttt acgccggggg tgaggtagag
     5641 agaatgacgg tctcggtcat ggtagacgga gatcaggacc aggccttgct caataagatt
     5701 gaagagtccg tccaaaccgc tatcggttat aacccggaac gcggtgatca ggtttcagtt
     5761 aacagcttta acttcgataa cagtctcgaa caggaaatag ctcaagctaa agaagctgaa
     5821 gcaagtgctg aacgcagcag gatgtatata tacggcggat taatcttatt aatcataata
     5881 attttaagtg taataatatt tttcattaga aggacaatga gctcctctga agccgttcag
     5941 acggtccaga gaggccaaat ggtcgactat caagcgggag aagctgaaga aactaaagat
     6001 tatagtaagc cgaccttaag tgaagaagaa aagcagaaga aacaattaac agaagagtta
     6061 gaagatatag ttgccgacca accggaagat gtagctaagt tactcaaaaa ctggttgatg
     6121 gaagactaaa atattgaaga gtaaggggtt ttattatgtc aggtatgtct gaattatcag
     6181 gtaaacaaaa agcagcagtt ttgcttattt cgctgggacc agatatgtca gcgaagatat
     6241 ttaaacacct ggataatgaa cagattgaag aagtaacact agagatagca aatatgcaga
     6301 aaataggaac cgaactaaaa aataaggtat taaaagaatt ttaccatctg ctgcaggcta
     6361 atgactacat cagcagtggt ggaataaagt atgccagaca gatcttaagt aaggcagtcg
     6421 gtgaaaaaga agcagataac atcttaaata gattgactgc caacctgcag gtaaggcctt
     6481 ttgataatat aagaaaagcc gaccctgatc agctccttaa tttcattcag ggtgaacatc
     6541 ctcagacaat cgctttgatc ctatcatata ttgagccttc ccaggcttct cagattttat
     6601 cttccttatc agaagaggtt caaagtgatg tagcaaagag aatagcattg atggatagaa
     6661 cttctccaga gatcataaaa gatgtagaaa atgtattaga gaagaaacta tcctctgtca
     6721 ttacaaatga gtatgcatca gccggtggta ttgactctat cgttgatatc ttgaatcagg
     6781 ttgaccgcgg tacagagaag aatatctttg atgaactgga tgaaaaagat cctgatctat
     6841 ccgaagaaat caagaagaag atgttcgtat tcgaagatat cgtattactc tctgataagg
     6901 ctatccagct tgtcttaaga gaggtcgaaa cccacgatat cgcgcttgct ctaaaaactt
     6961 ccggagaaga agtggaagaa gctatcttta acaacatgtc cagccgagct cgcgatatgt
     7021 tacaagaaga catggaatac atgggtccag tccgaatcag agaagtagaa gacgctcaac
     7081 aacgtattgt taatgtaatc agacagttag aagaaagcgg cgaaatcatc attgcacgcg
     7141 gtggggagag tgagttagtt gtctaagata attaaagctt ctcagatcat tggtaaatat
     7201 aaattatcga atgtacatga aagagatttt gaagtaaagg aagaaacagc agctgataac
     7261 aagggaaata ataaaaaaaa tactgaagaa aaccaaaaag aaaaagcaga agcaataata
     7321 aaagaagcgg aaaacaaagc taaaaaaatc atagaagaag ctagaaaaga agaacaaaag
     7381 atcgaccaaa taaaaaaaga agcttaccag gaaggttatg aacaaggaaa aaaagatggt
     7441 tatcaagaga caagtgggaa aatgcaggaa atcatcaata ctttaaatca agaaattgaa
     7501 gaactaaaaa ctcagtatga aaaagagtta aatcaactgc agccccagtt aataaatatt
     7561 gccagtcaga tggcttcgaa aataataaac cacaagatag aagaaacgcc cgaagtaatc
     7621 aacgatatag tagaagatgt tatgcagga
//
LOCUS       DATGCJ010000060        60900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_229798, whole genome shotgun sequence.
ACCESSION   DATGCJ010000060 DATGCJ010000000
VERSION     DATGCJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 60900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60900
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229798"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..783)
                     /locus_tag="VKN64_02965"
     CDS             complement(190..783)
                     /locus_tag="VKN64_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMA59228.1"
                     /translation="MGKVIYWVSYVLLNLYFKIVHHWRVKGRNNVPKEGPLIVVSNHV
                     SYLDPPIVGCAVNRQLHFMAKQELFDKKWFGFILHQIGSFPVKRGTPDKKAIRRSLEI
                     LKNGKVLCMFPEGTRIKGDKLGDAKPGVTLIALMSESPILPVGIKHSDDDSSLKVSVG
                     EPFSLDQYYDKKLSREEKSEVGEIIMKKIKEELDSIE"
     gene            complement(789..1451)
                     /gene="cmk"
                     /locus_tag="VKN64_02970"
     CDS             complement(789..1451)
                     /gene="cmk"
                     /locus_tag="VKN64_02970"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMA59229.1"
                     /translation="MTTSVAIDGPAGAGKSTVAKKLAEKLNFTYLDSGAMYRAVTLAA
                     LEDGVDVNNKEKLKRLVKDLSIDIEYKNNNFKIYMDDQDITNEIRTEKVDNNVSAVAK
                     IKAVRDELVKKQRMIAQTKDIVMDGRDIGTRVLPQADYKFYITATVKERAKRRYKDVV
                     NRGEDKSFEEVQQEIIRRDRIDSNRKYSPLKKAEDAVVIDTTDLNKEQVLKKILRIIR
                     GE"
     gene            complement(1554..1769)
                     /locus_tag="VKN64_02975"
     CDS             complement(1554..1769)
                     /locus_tag="VKN64_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59230.1"
                     /translation="MTVSLIEVLRIKESDRLFVLMSAFDILNLNMKTCKDDFEIRGPQ
                     KIEESGVLDPKMDHRMAMIFTVLRLIT"
     gene            complement(1916..2905)
                     /locus_tag="VKN64_02980"
     CDS             complement(1916..2905)
                     /locus_tag="VKN64_02980"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HMA59231.1"
                     /translation="MIIIYEGKNENYINFVDFLKDEDINYLKRKRDDLIAIIIISDIS
                     KKFVNIIRTTYKPDNILEEDEAHQFLDETRFIEIEGKKAFQQADFEIIAGPCSVENKV
                     QMEEIGQILSDLNIEYIRGGAFKPRTSPYQFQGLEEKGLQLLKDEAQKHGFKIVTEVM
                     DTDKIPVVSEYSDILQIGSRNMYNYSLLKEIGKTDKPVLLKRGMSATYEEFVMAAEYI
                     YRQGNKNIILCERGIRTFVEKTRFTLDLMAVPILKKLSDYPIIVDPSHGTGRWDLIQP
                     AAAAALTLGAQGVMVEIHPEPHLALSDGYQSLNLSNFKKLVKNIKKKTKRTGN"
     gene            complement(3044..3280)
                     /locus_tag="VKN64_02985"
     CDS             complement(3044..3280)
                     /locus_tag="VKN64_02985"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF019356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMA59232.1"
                     /translation="MYTIRGAITVENNQEDEILNAVKDLIKKIVKVNNLQKEDIISFI
                     FSTTEDLDSIYPGQGGKEVGVSKNLYYVSTGDVC"
     gene            complement(3336..4043)
                     /gene="rnc"
                     /locus_tag="VKN64_02990"
     CDS             complement(3336..4043)
                     /gene="rnc"
                     /locus_tag="VKN64_02990"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMA59233.1"
                     /translation="MTEYYSVDKLVELESKLNIKFSNKKLLRRALTHKSFANENAELK
                     IKDNERLEFLGDSVLSLSISTYIFNKYDDYKEGKLAKIRSVIVSAPVLAEKASELNLG
                     KFLLLGKGEELSGGRNRDSILADTMEAILGAIYLDKGFDFAFEFIISNFKSFIYEVDN
                     GNYIQDYKTMLQEVIQKNSNNRPEYEVVKEKGPDHNKEFYVVVNFEERELGFGKGKSK
                     KEAEQEAAKHALKQLDK"
     gene            complement(4138..4371)
                     /gene="acpP"
                     /locus_tag="VKN64_02995"
     CDS             complement(4138..4371)
                     /gene="acpP"
                     /locus_tag="VKN64_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMA59234.1"
                     /translation="MADVFDRIVKVVSEELAISEDEITEDASFIDDLGADSLDVVELI
                     MALEEEFDIEIPDEDAEEIATVSDAVDYIEGNL"
     gene            complement(4388..5131)
                     /gene="fabG"
                     /locus_tag="VKN64_03000"
     CDS             complement(4388..5131)
                     /gene="fabG"
                     /locus_tag="VKN64_03000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMA59235.1"
                     /translation="MLLKDQVAVVTGSSRGIGAATAIKLAEEGADVVVNYNNDKESAE
                     ETAQKIRDLGRQALVIQGNVAEMDDAKNLIKATVKEFDKIDILVNNAGITKDGLLLRM
                     KEKDFNDVINVNLQGTFNCTKQAIRYMMKQHHGKIVNLSSVVGLMGNAGQANYSASKA
                     GIVGFTKSVAREVSKRGINVNAVAPGYINTDMTEELPAKVKEDMLKNIPLDRFGEVED
                     VANVIMFLVSPLANYVNGQTINIDGGMLM"
     gene            complement(5147..5956)
                     /locus_tag="VKN64_03005"
     CDS             complement(5147..5956)
                     /locus_tag="VKN64_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HMA59236.1"
                     /translation="MYVYALVGVSGSGKSHRAAFVADKYDIPLILDDGLLIYKGRVLA
                     GKSAKRDKRKIQAIRTAILLEEDHLNEIKEALKDVDENKILILGTSMRMIERIVANLK
                     LDGIDEIIKINDIAKEDEIKKAIDTRKNEGKHVIPVPKIEVKSRFPGYFIHSLELFFG
                     KGKGSIKKEKTIVRPKFSYFGNLVIYNHVIRDLISFELSKEKIIIEVNSIHIEKEDNE
                     ICVNIEIILKYGVEIVEYINSLQVKLNELIEKFTGLYLDNLNFYVSSLYVE"
     gene            complement(6228..7127)
                     /locus_tag="VKN64_03010"
     CDS             complement(6228..7127)
                     /locus_tag="VKN64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HMA59237.1"
                     /translation="MKDFVVAGVQISVKPDNIEYNINKIITWLNKAVDEHNAELIVFP
                     ESITTGFAPGCSPEELWDKIDYVPGKLTGPIAKAAKKNSVFVVLPTYERGEKRGVVYN
                     SAVLIGPDGNIIGKYSKTHLFPSERLQGGGWTTPGNKVEVFETDLANIGITICYEGDF
                     PELSRILAIKGAEVIVRPSALLRSYDIWEMTNKARAYDNHIYMIGVNSVGADAEENYY
                     FGHSMIISPIAEKIALARGTEEIVSARLDSDPIKHVSYGVKSPMIFDHLEDRNLSVYD
                     GLTKEAKSKFEPYERIPYKKDND"
     gene            complement(7139..8299)
                     /locus_tag="VKN64_03015"
     CDS             complement(7139..8299)
                     /locus_tag="VKN64_03015"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HMA59238.1"
                     /translation="MIIINNKKPLEDLVVLDLSRVLAGPFCTMLLKDLGAEIIKVERP
                     ETGDDSRHFGPFIEGRSAYFISLNWGKKSITLNLKDEEDRNTFLGLVKKVDILVENFR
                     PTTMEKLNLDYNTLKEYNEKLIYAAISGFGHSGPYSDKAAYDMIVQAAGGIMSITGEP
                     DRPPVRVGASIGDIVAALFATVGILAAVHERKKTGKGKKVDIAMLDSQIAILENAIAR
                     YTASGEAPGPLGNRHPSITPFQAFKAKDEWFIIAVGNDNIWAKFCEAIGREDLIDDPR
                     FKTNDKRTENREELVEIINDFVIDKKADDVIELLDNHGVPAGPINDVEDLFNHPQVEA
                     RNMLVDVVDPDDGRFTIAGNPIKLSDTQDETERERAPKLGEHTEEIIKKYLG"
     gene            complement(8585..10909)
                     /locus_tag="VKN64_03020"
     CDS             complement(8585..10909)
                     /locus_tag="VKN64_03020"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HMA59239.1"
                     /translation="MSEEMIGKSVNRIKALEKVTGSATFVHDMELQGMLHAKMKLSPH
                     AHAKIVNIDSSEAEKLPGVWTVLTGKDLPYKVGLYLVDKDILAVDKVRYQGEPVVAVA
                     AETEEIAEKAIELIDIEYEKLEAALDVKEAVKEDAPLVHENLGELESAPVFFPKPGTN
                     IAHHTKIRKGDIEKGFEEADYIIENEFELPMVDHTALETHTSIAKWMPGKKVKIWTSA
                     QSPFAVRNLLANGLHLNHEDIEVQIPYVGGGFGGKAGIHFEPLVTCLSRASQGRPVKI
                     VMSRMEEFNSVPVRQGLYSKLKTGVTKEGKIVAFESEYLWDSGANADYGVNVTRAAAT
                     AGAGPYEVDNVKLDSKTVYTNKIFGTAYRGFGHLEILWAVERQMDLVAEEIGMSPYDI
                     RMKNGLKRGSVTITGEHIGESHGKVLECLEAVAEEIDYENRSSDDLPDGKKRGKGLAL
                     LHKAPAMPTDTGTAAFLKFNENGTLNLTVSLTDYGQGTYTALAQIVSDQLDIPIDKVN
                     VVWEQNTETQPYDWQTVASKGLFMSGNATIKACQDMAQQLKEVASEPLRCSVEDLIIE
                     DEKVKIRHHKDKYISFKDLAMGYSYPNGNAIGGPLVARGNYIAQGLTNIDPETGQGLP
                     ALDWTYGAHGVEVIVDEETGELEVIKIASAFDVGKTINKQNVIGQIEGGVIQGYGSAV
                     MEGYQFSDDGRLLTNTFTDYKVPSIADIPELKPIIIENAQEDGPYGARGVAEHPMLSI
                     PSAVGNAVQDAIGIHIKEMPVIPERIYDYIEKEK"
     gene            complement(10912..11382)
                     /locus_tag="VKN64_03025"
     CDS             complement(10912..11382)
                     /locus_tag="VKN64_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA59240.1"
                     /translation="MKTINLTVNGRIFELEVQENEILLDTLRKKLNFTGAKEGCGEGE
                     CGACTVIMDGKAVTSCLILSVEADGSEIETIEGQSIGEEISDIQKAFIEENAIQCGFC
                     TPGMVMSTKALLDRNPNPSDEDIDIALSGNICRCTGYYSIRNAVKRAAEKKGGN"
     gene            complement(11382..12236)
                     /locus_tag="VKN64_03030"
     CDS             complement(11382..12236)
                     /locus_tag="VKN64_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HMA59241.1"
                     /translation="MLNKFDYFKPTKLEELFEILDNNEGESALLAGGTDLFVDMKHKT
                     RKPDYIIDVKGIKDFGKIEERDAGLFIGGTVTCNEIIDSELVNNDYKILADAALEVAA
                     HQVRNRATVVGNLCTTSPGADMFPSLLVLKTTVLIESKEGSREVPLDEFATGVKSNVL
                     KDKEVVKGLIVQKHAEDGKGAYLKKRRTKGPDLSGIGVAGYLSKAEEYLAFALGAVAP
                     TPVYVDVSDLLFADGSFEEKQGKIVNRILDNINPIDDVRSTKKYRKKLSEVYTKRILS
                     SLWEAGDK"
     gene            complement(12268..13200)
                     /gene="arcC"
                     /locus_tag="VKN64_03035"
     CDS             complement(12268..13200)
                     /gene="arcC"
                     /locus_tag="VKN64_03035"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HMA59242.1"
                     /translation="MKLTIALGGNAILQPGQDGTAKEQFENVLSTSKQIGQLVNDGHE
                     VVITHGNGPQVGNILIQQDKAKDSVPAMPLDVCGSQTQGFIGYMLQNSLHNVFAELGL
                     DRNVATVVTQVLVNKEDSAFDNPTKPVGPFYDKEHADKMVKEKGEDWIEDSGRGWRKV
                     VPSPIPEKIIEAPIINDLVRNGEIVIASGGGGIPVVNKDNKIHGVEAVIDKDRAGCLL
                     AEEIDSDIFLVLTDVSNAYINYGKKDEEAIGEIKVNEMEEHIEKGHFGEGSMKPKVQA
                     ACDFVESGGEMAIITSIEKVLEAVHGNAGTRITK"
     gene            complement(13284..14042)
                     /locus_tag="VKN64_03040"
     CDS             complement(13284..14042)
                     /locus_tag="VKN64_03040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMA59243.1"
                     /translation="MKLYDLTQKIGVSTPPWPTYEPLELKYFKRLSTERVNGQIIKTS
                     NHIGTHLDGERHFWTAGRDMASIPLEDLFGEGVIVDLSDAVEDYDIYTSEMIEERVEV
                     KEGDVLIINTGYHKYGWDQPEADEERYFMKHPGPNMEFAHWAMEKKLKWIGVDCGSAD
                     HPMNTVCKKLRPDLAKEAEEHLGAPLDEVFSHPESRQLMHTKLFPEGIIHAENIGGEI
                     DKILNKRCKIGFFPWKGVDLESSIGRAVAFVEEE"
     gene            complement(14058..15482)
                     /locus_tag="VKN64_03045"
     CDS             complement(14058..15482)
                     /locus_tag="VKN64_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HMA59244.1"
                     /translation="MGKKLEDLFKEDLKVINIGLESFSENLKDEGYDAVQVDWKPPAG
                     GDKEMAALLDDIEKISDKIEKANEEAVERMQKSEPVVVDVQTAKDVLPNFEGKMILHA
                     APPEKWEDMSGPMQGAAIGACIYEGWAEDEESARAMLDAGEVTFDPAHHHNAVGPMTG
                     IVSPSMPVWVVENKTYGNTAYTTLNEGLGKVLRFGANGPEVIEKLKWMEKVLGPAIGE
                     ALRKTEDGINLKNITSQALHMGDEVHNRNKAGTSLFIREITPYLLQTDYDNETIKEII
                     EFMSGNEHFYLNLSMPASKATMDAAHGIEYSTVLTAMARNGVHFGIRVSGLGDDWFVG
                     EAQKVDGLYFPGYTEEDACRDLGDSTISETAAVGGFAMAAAPAIVGFVGGTVSDSIKF
                     TRDMYEITVTENENFTIPPLNFRGTPTGIDLRKIIETGILPAINTGIAHKEPGIGQIG
                     AGLVDPPEECFKKAIRAFAQKYIK"
     gene            complement(15497..17050)
                     /gene="fdrA"
                     /locus_tag="VKN64_03050"
     CDS             complement(15497..17050)
                     /gene="fdrA"
                     /locus_tag="VKN64_03050"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HMA59245.1"
                     /translation="MAKKALIKEGTYFDSVTLMSISKDVKAINGVKEATIVMGTEANK
                     GILESAGLLVPEAESAKPNDLVIVIDGEEEVLNKAIEKTNEMLAEQKDSSDKEKEYKP
                     GSLDGALDVQPDANLVIISVPGEYAAREARKALKKGLHVMLFSDNVPVEDEIELKKIA
                     SKKDLLLMGPDCGTAIINGAPLAFANVVNKGPVGIVAASGTGAQAVSSLIDRQGVGLS
                     QVIGTGGRDLKFKVKGVEMIKDMKALDKDDETEVIVLISKPPAEEVSKKIMDEVKKLS
                     KPVIVNFLGGDPSVIEGTDAIWADTLEDAALKAAAFVKGEDKVSAAGLTKEEIAEIAK
                     EEVNKMSSEQKYLRGLYTGGTLCSEAVILLSEKSGDIYSNIPLKEELGLKEDLKSIKD
                     TVIDLGEDEFTRGKPHPMIEPSLRNERLIKEADDKETAVILLDIVLGYGSHLDPAGEA
                     AKVIKEAKEKVTERGGYLSVIASVCGTDKDPQNLQAQEEKLREVGAKVLPTNVQAVRV
                     VNKILSDLK"
     gene            complement(17268..18398)
                     /locus_tag="VKN64_03055"
     CDS             complement(17268..18398)
                     /locus_tag="VKN64_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HMA59246.1"
                     /translation="MVQVFIEFFQKTGIMALDIPQIIMIIVSLVLLYLGIVKKYEPLL
                     LVPIAFGMLLVNLPMTNLMIHAADGKPAGLFRYFYTGNALGIFPPLIFMGIGAMTDFG
                     PLIANPKSLLLGAAAQFGIFITFLGAILLGFTGQQAAPIGIIGGADGPTAIYLSGQLA
                     PEYLGAIAIAAYSYMALVPVIQPPIIRALTSKKERKIKMQQLRPVSKSEKILFPILVT
                     IVVLLLLPDAAPLIGMLMLGNLFKESGVVDRLSETAQNSLINIVTILLGVAVGASASA
                     DKFLKLDTIQIIILGVLAFAFGTMTGVLLGKLMNNFSSDKINPMIGAAGVSAVPMAAR
                     VVQKEGQKADTSNYLLMHAMGPNVAGVIGSAVAAGVMMSIFA"
     gene            complement(18426..18842)
                     /locus_tag="VKN64_03060"
     CDS             complement(18426..18842)
                     /locus_tag="VKN64_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HMA59247.1"
                     /translation="MTKKFKVKVNDELYEVEVEEVETDQQSVVQSETQNREHAPQPKT
                     STSTAETSSKAKKKPRKSASSSRGAGTVEAPMTGTVLEVKVKQGEEVTKGDLLLVLEA
                     MKMENEVYAPTSGQVSEVNVDENQKVEAGEVLVVIE"
     gene            complement(18839..19249)
                     /locus_tag="VKN64_03065"
     CDS             complement(18839..19249)
                     /locus_tag="VKN64_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HMA59248.1"
                     /translation="MNPLELLRDPNLVQSMTLNQKLISGLQVAILGMLIVFLILFLLM
                     IVVNILKKIFYSEKIDFKEDAKKVEPKVADLEPDIDHKKEVAAIMAAIMSIESSSKQK
                     KFKIKKITRVKDDVPIWGRVTRGGISANNKGELL"
     gene            complement(19270..20814)
                     /locus_tag="VKN64_03070"
     CDS             complement(19270..20814)
                     /locus_tag="VKN64_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMA59249.1"
                     /translation="MKDKIKELHKKRGEVSKGGGEKRIEKQHSKGKLTARERIDRLLD
                     EGSFQEIGMFVEHHSQDLGMDEREIPADGVITGQGTINGRSVYLFAQDFTTMGGSLGE
                     MHAKKICRIMDLAQKSGSPFIALLDSGGARIQEGVNALKGYGEIFKRNTDISGVIPQI
                     SVILGPCAGGAVYSPAITDFVFMVDHTSKMFITGPQVVKSVTGEEVSAEELGGGSTHN
                     EVSGVAHFLADNEEHSFEQIKRLISFIPNNNLEGAPVVETNDSPERVEDKLKDIVPIN
                     PNKAYDIRDVIKKVVDNQDFFEVHKYYAKNIVVGFARLNGRSVGVIANQPRVKAGTLD
                     IDSSDKAARFIRFCDSFNIPIVTFTDVPGYLPGVEQEYGGVIRHGAKLLYAYSEATVP
                     MITVILRKAYGGAYIAMSSRHLGADYVFAWPNAEIAVMGPQGAANIIFRKEIKKAENP
                     EEKRQEKIEEYRESFANPYRAAAEGYIDDVIDPRNTRPRIIMALESVIGKRENQPNKK
                     HGNIPL"
     gene            complement(20936..21772)
                     /locus_tag="VKN64_03075"
     CDS             complement(20936..21772)
                     /locus_tag="VKN64_03075"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HMA59250.1"
                     /translation="MFDYIIEGDLVKENYSLNGNIISKFNNVINIKTKNNKGYAITDS
                     KVTLAPYHVKLPNYIFNNIKNSLEEETKIKINNEQIIYNKNIFNYNNKNIFSGKIKDC
                     SFVDRDLLIEWTEKVIKNTNRSGSLDHTILKFTNIINNIQEFKLSTLQREFYNKLKDI
                     LEKKISINNLLGLGIGLTPTGDDFIVGYLSVIEAKLAEDKLNVINEMETDELFDKTNR
                     VSALHLKGAINHRYNKRLVDIYKNIENGEKYYRKYTEKLIEMGNSSGLDMLSGLYFGL
                     IM"
     gene            complement(21765..23432)
                     /locus_tag="VKN64_03080"
     CDS             complement(21765..23432)
                     /locus_tag="VKN64_03080"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HMA59251.1"
                     /translation="MKELIGITMADALKLEKMKEVEILAGKDGLERHITKVNIMEVPD
                     ILDWVKEGELLFTTLYSIKDDEKALKNLIPNLAQKNLAGLGIKPGRYINEIPSFMIEQ
                     AELHNFPLLKIPYDYSYSVFINPILSEILNVQTRFLEKTLNIHEVLTNTVLYENGLDR
                     LSTVLVDMIKNPVLITDSNGAIISYSIPDDFNNEVTKKDLTDSISINDQKESKEEVFN
                     YHCKKIDVTLHGKEINLMKIPINTPNSHFGYLFAWEYGKSINELDISTLKWASTIAAL
                     DILNKRSIANVELKYKNELLYDILKGKINNKKTILNRGQNMDMELDKGFSVVVFELKE
                     LISKHFKKKGILNENIQKSYLESANRIIDEEVIIGDLGTYLVALYPGKEKKSIKEFSQ
                     KVLNVFDQKDRDLVTVGVGNYKKEIINLHESYSQAKRTINVANKLNKKDQVYFFDDLG
                     VYKLLYKVDREEKNRFLKNSLIPLLKYDKAHNTELLKTLKAFFEENGNLTNVAKKIYV
                     HYNTVHYRLKRIEKITGLSLDNPDDKLNLEIALKMLNFTDILGNENV"
     gene            complement(23655..24902)
                     /locus_tag="VKN64_03085"
     CDS             complement(23655..24902)
                     /locus_tag="VKN64_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HMA59252.1"
                     /translation="MESREEQAVRDVSNLPKSQAIFLGLQHALAMFGATILVPILTGL
                     HVSVALFTAGVGTWLFHFITKKKVPAYLGSSFAFIAPISLVIEQHGGIPAAQGGIIAA
                     GLIYAIVAFIIYKVGPSLIKKIFPPVVTGPVIILIGTILAPTAIDMSTGSALNVVVAI
                     AALAGAVIAAIFAKGFFKLVPIIVGLVTGYIVAVIVGMVDFSPVINNAWIGLPEFTVP
                     AVNITAITIIAPVALVSMVEHVGDILAISETIGKGRELVTDPGIHRTMFGDGMATSLA
                     GIFGGPPNTTYGENTGVLAITKVYDPVVMRIGATFAIAMAIVPKFGGLIETIPNPVIG
                     GISFLLYGMIATIGIRTLVESQTDLKISRNLVIVATVIVLGLGGATLQLGRIEFSSLA
                     LAALVGVVLNLILPDGEEEFAQM"
     gene            25191..25673
                     /locus_tag="VKN64_03090"
     CDS             25191..25673
                     /locus_tag="VKN64_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA59253.1"
                     /translation="MEGIKLLKSRRSIREFKEQKVEKEKLEKIVETARYAPTANNVQP
                     WQFIIVTDENKIETLEKYIGQGKEFPACIAVFTKDVKHHLEDGSNATTYILLAAEELK
                     LGTCWIGSYGKPYSSEIKELLNVPENYKLISMVTVGYPDENPHPDKKELDDVINWEKF
                     "
     gene            complement(25732..26982)
                     /gene="fabF"
                     /locus_tag="VKN64_03095"
     CDS             complement(25732..26982)
                     /gene="fabF"
                     /locus_tag="VKN64_03095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMA59254.1"
                     /translation="MSNRVVITGLGVVSSLGMEIDEFWNNIINGKSGISKIEGFDPEG
                     YSSQIASEIKDFDPGDYIDRKDAKRMAKFTQYAVYAAMKAVENAGLEVTDDIANDTGV
                     IIGSGIGGIEVFEKQMERLVKRGPRRISPFFIPMMISNMAAGQVAIYANAKGPNSNEV
                     TACASGTHSIGEAMEIIKRGDAKVMISGGAEASITPSAVAGFSNMKALSTRNDEPTKA
                     SRPFDKERDGFVIGEGSGILVLEDYEFAKKRGANIIAEISGYGMSADAYHMTQPAPGG
                     EGAYRAMEMAVNKANVAYEDIGYINAHGTSTPFNDKLETKAIKNLFKEHIENLAVSST
                     KSMTGHLLGAAGGIEAIVSALAIKNDILPPTVNYENKDPECDLDYVPNKAREVSDLKA
                     VLSNSFGFGGQNACLVINKIEENY"
     gene            complement(27002..27931)
                     /gene="fabD"
                     /locus_tag="VKN64_03100"
     CDS             complement(27002..27931)
                     /gene="fabD"
                     /locus_tag="VKN64_03100"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMA59255.1"
                     /translation="MSENIAFLFPGQGSQYVEMGKDFYDNFAVAREYFDKANQVLDID
                     LKKLCFEGPEDELTETQNTQPAIFTVSMIAYEILKDKGVEPSMVAGHSLGEYTALCAA
                     GVFDFETGLKLVRKRGELMASAVADGSKGTMAAIIALDREKIEEICGKVAGVCELANI
                     NSPMQIVISGEEEAVHTAMEKADEAGAKKIVELNVSSAFHSQLMEPAKNKLKEYIQSV
                     QFKDPKVPVIANSTADFVKNKEEIVTALEKQLINPVRWVESMELMGENGIECALEVGP
                     GRVLKSLMRRIDRSIKTYNIQDSASLEKAMKKI"
     gene            complement(27950..28939)
                     /locus_tag="VKN64_03105"
     CDS             complement(27950..28939)
                     /locus_tag="VKN64_03105"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMA59256.1"
                     /translation="MRRATITGWGKYLPPNVMNNKDLEKIVDTTDEWIRKRTGIKERR
                     IADESTSTSDLALKATYEALNKANLDPKKLDLIIVSTVTPDMAFPATACIIQDTIGAE
                     NAAAFDLEAGCSGFVYGLSVGAQFIESGLYDNVLVIGAETLSKITDWDDRNTCVLFGD
                     GAGAAVLQPTEKGGFLSFDLGSDGSGADALYMEAGGSKKPASLETVKNRQHYIRMEGN
                     PVFKFAVKTMKNASKDVLKKANLTTDDIDLLIPHQANTRIIKSARRRLKLDEEEVYVN
                     LPKVGNTSSASVAIALTEAVEKGKIQNGDHVLLVAFGAGLTWAAGVLEWNQNI"
     gene            complement(29066..30016)
                     /gene="fabK"
                     /locus_tag="VKN64_03110"
     CDS             complement(29066..30016)
                     /gene="fabK"
                     /locus_tag="VKN64_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HMA59257.1"
                     /translation="MYLDTRICDLFDIKHPILQGGMAWVATGELAAAVSKAGGLGIIG
                     AGNAPKEVIKEEIKKVKDITDNNFAVNIMLLSPFVDDIVDLVIEEEVPVVTTGAGNPG
                     KYIERFKANNIKVVPVVSSVALAKRLVRSDIDAVIAEGTEAGGHIGDLTTMTMIPQLV
                     DAIDVPVIAAGGIADGRGLAAVLSLGASGVQMGTRFVCAEECTAHKKYKKAILSARDR
                     DAVVTGRSTGHPVRNLKNKLTRKIDNMEKEGIDKKTIEEMGSGKLREAVREGNIDTGS
                     VMAGQIAGMIEDSKPVQEIIDDIINETIEVINKNKDYIRE"
     gene            complement(30038..31042)
                     /gene="plsX"
                     /locus_tag="VKN64_03115"
     CDS             complement(30038..31042)
                     /gene="plsX"
                     /locus_tag="VKN64_03115"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HMA59258.1"
                     /translation="MVLKIAVDAMGGDKAPEEIIKGVVKAVDEFKDIKCILTGKENKI
                     KSILEQLNYNNESVEVVDAKQTVSMADKPSKVLRRKKDSSLVVGTNLVKENKADALIS
                     AGNTGAVMAAGIFKIGRVSEVKRPPIATVFPSKIGKTVVLDAGANVDSKPQHLIQFAI
                     MGQIYAEQVLNINNPRVGLLSIGEEKEKGNKLNAESYELFEEEERIDNFIGNVEGRDI
                     FNGSCDVIVCDGFVGNIVLKTTEGVASYIFDLLKDTLTSNLKAKIGALLLKDELKKMK
                     DKVDYRQYGGAPLLGLNGLVIISHGSSDAKAIYGAIKVAREAVKNNVVEIIESKLEIE
                     "
     gene            complement(31172..31345)
                     /gene="rpmF"
                     /locus_tag="VKN64_03120"
     CDS             complement(31172..31345)
                     /gene="rpmF"
                     /locus_tag="VKN64_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMA59259.1"
                     /translation="MAVPKKRTSKSRKRKRRTHWKLKSPNLVECPECNELILSHRACP
                     SCGYYKGEKVINT"
     gene            complement(31371..31841)
                     /locus_tag="VKN64_03125"
     CDS             complement(31371..31841)
                     /locus_tag="VKN64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HMA59260.1"
                     /translation="MILDLSDLMEVGSKREYVKELDIDHIDFRHKEIEIEGPLKVNLD
                     VLKAEDSFVLTGQIEGTLILECSRCLKEFPYPIEVEISDELFIEDIEDLHNVNVFPIL
                     VSDIILEIPIKPLHDEDCKGLCSVCGQDLNEGECDCEEEIVDPRLAKLKDFKQK"
     gene            complement(31909..33108)
                     /locus_tag="VKN64_03130"
     CDS             complement(31909..33108)
                     /locus_tag="VKN64_03130"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HMA59261.1"
                     /translation="MKVLVLNSGSSSIKFQLFNMEEERVLAKGLVERIGIEDSAIEYE
                     NDNGQEVEKDIDIANHKEGIEHVIEILLDPEVGVLEDIDEINAVGHRVVHGGEKFAHS
                     TLITDEVIEKIEEVTPLAPLHNPHNLSGIKVCKELLPGKPQVGVFDTAFHQTMPEEAY
                     IYAIPYEYYEEYGVRRYGFHGTSHKYVSNRCAELMDKDAEDAKIITCHLGNGASIAAV
                     DGGDSVDTSMGMTPLEGLVMGTRSGDIDPAIIPFLMEKEDLDIQDIDNILNKKSGLLG
                     VSGVSNDSRDVRQAAHNGNHRAALADKVFNYRVRKYIGAYMAALNGVDAIVFTGGIGE
                     NAGGVREHILEKMDNLGIDLDSQANKVRGEEKEITTSESDIKVFVIPTNEELVIAQDT
                     KEIIENQ"
     gene            complement(33139..34137)
                     /gene="pta"
                     /locus_tag="VKN64_03135"
     CDS             complement(33139..34137)
                     /gene="pta"
                     /locus_tag="VKN64_03135"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HMA59262.1"
                     /translation="MDVMKEFKNKARESKKKIVLPEGYEPRMIKALPKIKEENLADII
                     LLGEEAKIREIAEQEQVKLPDVQIINPAKSELKEDFAEEFYELRKHKGISRDDALDKI
                     VDPLYFGVMLVHTEKADGMVAGSINATGDVLRPALQIVKTKENISVVSGAMIMDVPDT
                     EYGHEGIFIFSDVAVNPNPDAKQLAEIAVSSADTAGLLPGVDPVVAMLSFSTKGSAKH
                     ELADKVIEATELAQKMEPDLPIDGEMQADAALVPEVGEQKSPDSDVAGRANVLVFPDL
                     QAGNIGYKLVQRLAKANAIGPILQGMAKPVNDLSRGCSVEDIVNVVAITAAQAQNL"
     gene            34281..35552
                     /locus_tag="VKN64_03140"
     CDS             34281..35552
                     /locus_tag="VKN64_03140"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HMA59263.1"
                     /translation="MKVTGIIVEYNPFHKGHQYHINKARAITKSDYIIAVMSGHFTQR
                     GTPAVCDKWNRTKMALESGVDLLIELPLCFSIRSAEYFAQSAMQLLAKTNLVDDVVFG
                     SEHGDIDILKNIASLLLENNQYYEKRLKKYLKEGSGFPAARKNALLDLVKLKSNTNYW
                     SQHNIFAILNGSNNILGIEYLKAKQRFNLNLEVDTIKRIGPNYYSEEKTDHYVSATAI
                     RNSLNNNELDSVKEKIPAITYKILKKEIADNKIPIKKENLGLILLAKIRKLSVEQLVK
                     ILDINQDLAVRIKNAANNSGNYKQLMASINTRSYTKTRFQRILLNILFNINKDIINNH
                     DYKGPSYIRILGFNQKGKDLLSKMSEKSSLPIIINPSEYLNNLTFSSEDLLKNSLSLD
                     IYATDVYTLLYQNASYRKANLDFTKKVIKSF"
     gene            complement(35549..36562)
                     /locus_tag="VKN64_03145"
     CDS             complement(35549..36562)
                     /locus_tag="VKN64_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMA59264.1"
                     /translation="MRKDKLTKGIGILIILFIIINFIPTPYYVMSPGIAEELGPIITV
                     ENGHKDNIKGSFLLTAVGSQKATIWDYIYVNTFNPDGFELEILSEQLPEGIDMEKYLQ
                     MMSDLMEESKMTAEAVALRKLGYEVKVQGEGVEIIEVLEASQAYNELKNGDIIKNIDG
                     QKVEIASEAVDIIRNREVGDKVNIEVIRDNERLAFTVETIELSESPGKPSIGILIRTK
                     NLDYEFPIQVSYDTGNIVGPSAGGMFALEIYNQLVEEDLTDGKRIAGTGTINLEGEIG
                     QIDGIVQKIIAAENAEAEIFIVPQENYEKAKTVETNLKLVPVETFDDAIEFLKSQNLD
                     KAA"
     gene            complement(36593..37078)
                     /gene="coaD"
                     /locus_tag="VKN64_03150"
     CDS             complement(36593..37078)
                     /gene="coaD"
                     /locus_tag="VKN64_03150"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMA59265.1"
                     /translation="MLDRVVYPGSFDPVTKGHLNIIKRASNIFDEVIVAVFQNPNKEP
                     IFSMEERVRLLNETTQDINSVKIDSFTGLTTKYVKSKNACAIIRGLRAVSDFEGEFQM
                     ASMNKELDPEIETIFLMTDSKYAFLSSSIVKEVAQFGGKMDNVVPKPVEKALLEKYEI
                     E"
     gene            complement(37082..37627)
                     /gene="rsmD"
                     /locus_tag="VKN64_03155"
     CDS             complement(37082..37627)
                     /gene="rsmD"
                     /locus_tag="VKN64_03155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HMA59266.1"
                     /translation="MRVIAGKAGGIRLKSIDTKEVRPTLDRVKEAVFSMILPYFPFEA
                     GLDLFAGFGSLGLEAASRGTKHVTFIEKNRRYADIIRENIKKCGFEESCEVMTENVFT
                     YTQTVNKKHDIVFLDPPYKNNMVNKTLRSLINNELLRAGALIIVEHHRDEEIEDFEEF
                     KILKDRNYNETVIKVYFYEGE"
     gene            complement(37642..39696)
                     /gene="recG"
                     /locus_tag="VKN64_03160"
     CDS             complement(37642..39696)
                     /gene="recG"
                     /locus_tag="VKN64_03160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMA59267.1"
                     /translation="MSKTSAEEIRYLKGVGPKFEKRLNNLGIYSLKDLFYYFPRDYED
                     RGDFKEIRFIRPGQAVSIQAEVLNKELIRRGRYKSIFKVTFSDGTDTVICTWFNQAYL
                     KDVFEKGKKFNLYGELNENSYHQYNKKEIVNPVYEEIEEDSPTIHTGRVVPIYPLTEG
                     ITQKRIRYIIYQALQKYLKNIDDFLPDYIIDKHNYIEIRNAIFNMHFPQDRKTFVQAR
                     NRLAFQELFLFQIAMLKRKKDYRKQKGIKHSQTAGLIDDFLNQLHFDLTDAQKNVWQQ
                     IKADMENKHPMQRLLQGDVGSGKTIVAVMSLIKTIESGYQGILMAPTEILAEQHYIKI
                     KKILKNFDYNIELLIGSIKGEKREEITDKIKNSESDLIIGTHALFQKDIDYSNIGLIV
                     IDEQHRFGVEQRYQLKQKGDNPDLLVMTATPIPRSLALTLYGDLDLSIIDEMPPGRKP
                     VITTWRTEKSRGKIYNFVKDKIDEGEQVYVVCPLIEPSEDLDAYSVEEVKKMLEETYL
                     SNYIIKAMHGQLSNDEKNKIMSDFVNQKIDVLISTTVIEVGVDVKTANVMVIENAERF
                     GLAQLHQLRGRVGRSASQAYCIMIANPKTEEAKKRLEVMSETNDGFKISEADLEIRGP
                     GEFFGTKQHGVTDLQVADILKDTKILAKANREANELTNQKDWALKYDKLAEKLKELEI
                     II"
     gene            complement(39689..40576)
                     /locus_tag="VKN64_03165"
     CDS             complement(39689..40576)
                     /locus_tag="VKN64_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA59268.1"
                     /translation="MKIGILTDSTCDLPENILEEYNIEFVPLTVRIGEEQYKDKTEIT
                     SQQFFKRIKQADVMPSTSQPSAGEFEQKYREMNRQYDHILSIHLSSHLSGTKQSAQLA
                     IQKNPDYNITLIDSKSASMGLGFLVILAAKLVDKGLELENIVDIIKKAREDLEIYLTV
                     DDLSYLQKGGRINKAESFIGNLINLKPVLKLDFNSTSVEPIDKVRGRKNTHQRLLEFL
                     DDYLNKNLESPGQYWFGFLYGEEEYAANFREKFNSKIEEANIRHIYYENNVGPVIGSH
                     TGPTVYGIILFKGGKYLDE"
     gene            complement(40599..42290)
                     /locus_tag="VKN64_03170"
     CDS             complement(40599..42290)
                     /locus_tag="VKN64_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HMA59269.1"
                     /translation="MAQNEEIKVINGEAFKNMCYGALNRLEDQQSYIDSLNVFPVPDG
                     DTGTNMLLTFKEAVENLDKAKSERIDRLTQALSKGALMGARGNSGVILSQLLRGFSEA
                     NKNNRVMRNDDLINSLSKASKIAYQGVLKPVEGTILTVARKAARRAEKLEDEKNIEVF
                     LEKVIETAHKALNKTPDQLPQLKEAGVVDAGGQGYLTILEGMKAGLTGKVEVREEVKE
                     KREIKEEVAEELKYGYCTQTLINVKEQNVNIDEIKNDIEQFGDSLMVVGDDDIIKVHI
                     HTNHPGIILEYALKTGSLRDIKIDNMRMQNAEMRKKHEEEQKEDFEQLKDESTEDYEE
                     KLIKSAKSEQLDRKVGIIAVAKGKGIKNILMELGVDLIIDGGQSMNPSINDFVEAVEQ
                     MEQEKVIIFPNNKNIISAAQQASSLSDKEIKVVETKAIPEAIAALLVFNDNKELDELR
                     SSMEEEISYVKTIEITNAVKDSKVNGFEINEDDVIGIVNGDIRAVGNSEIEVISDLID
                     EVWEDEDLITIYYGDGASQQDAEEIKKKFQAKSKEAEVEIYKGGQPLYPYIISME"
     gene            complement(42277..42642)
                     /locus_tag="VKN64_03175"
     CDS             complement(42277..42642)
                     /locus_tag="VKN64_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HMA59270.1"
                     /translation="MVKEINTEYGKILIAEEVIETIAGLSAMECYGLVGMSSKNMQDG
                     IADLLGWDNLSKGVSVEIEEDAVNIILNIVVEYGTNIHEVANNIIERVKYTLKNRVGI
                     EVESIEINVQGVRIGNGTE"
     gene            42802..43032
                     /gene="rpmB"
                     /locus_tag="VKN64_03180"
     CDS             42802..43032
                     /gene="rpmB"
                     /locus_tag="VKN64_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMA59271.1"
                     /translation="MAKYCEICGKGSNNANRVRRRGKAKKKGGVGRNVTSVTSRTQKP
                     NLQKVKAIVDGKKKRITVCTSCLKAGKVKRAY"
     gene            complement(43082..44302)
                     /locus_tag="VKN64_03185"
     CDS             complement(43082..44302)
                     /locus_tag="VKN64_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMA59272.1"
                     /translation="MDTKEEALKLHKKLKGKLSIKSKQKIKNKRDLSLLYTPGVAEAC
                     QQIYKDEESIYDYTIKNNSVAIVSDGSAVLGLGDIGPKAALPVMEGKAVLFKEFADVD
                     AYPLCITRRNVDEFVQNIVNLSHSFGGINLEDIKAPYCFEIEEKLKAKTDIPIFHDDQ
                     HGTAIVVLAGLFNALKLADKKIEEIKIIITGAGAAGFAVTKLLKTAGAKNIKVLDSKG
                     VVYKGRKSNNEYNEKLAEMTESKKEGRLEEAIKGSDVFIGVSTAGILKSYMVKTMNEK
                     PIIFALANPEPEINPKEAYQAGAFIVATGRSDYPNQINNVLAFPGVFRGVLDARASEI
                     NEKMKLKAAEKIAEIVKDNLNKENIIPEVFNKEVAYVTALAVAEEAVKTGVAHQDLTL
                     EIITEKVKNNLSKK"
     gene            complement(44307..44858)
                     /locus_tag="VKN64_03190"
     CDS             complement(44307..44858)
                     /locus_tag="VKN64_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HMA59273.1"
                     /translation="MKLETPLTEKKVKKLRAGDKVELSGYIYTARDQAHKKLNDLIKK
                     NKKLPIDLKDAVIYYTGPAPSREGQVIGSCGPTTSYRMDEYTPALLEQGLSGMIGKGP
                     RSEVVKKAIKKNKAVYFAAVGGAAALLSKSIMESKIVAYPELQTEAIRLLKVKNFPVV
                     VINDIYGQDLYEIGQNKYKEELG"
     gene            complement(44869..45699)
                     /locus_tag="VKN64_03195"
     CDS             complement(44869..45699)
                     /locus_tag="VKN64_03195"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HMA59274.1"
                     /translation="MRRVDSEIITKEITDAVQTANFSLNKDMYNRLEQAVKKEESRLG
                     KNVLKELIENAKIAEKEKIPICQDTGIVVVFVEIGNEVYIEGSLEEAINYGVKIGYNE
                     GYLRKSVVETPFRRENTDDNTPAVIYIEDVPGDKLKLNVLIKGAGSENMSKIRMFNPT
                     ADKKDIQKFIIDVVKNAGANACPPYILGVGIGGTFEKAAYLSKKALLKKESKDEKINQ
                     FEEEILREVNKTGIGPAGLGGKVTALDVKILTYPCHIASLPVAVNINCHASRHFSIEI
                     "
     gene            complement(45713..46357)
                     /locus_tag="VKN64_03200"
     CDS             complement(45713..46357)
                     /locus_tag="VKN64_03200"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HMA59275.1"
                     /translation="MKEVLVVLNGVYKDQKEKYQEIVNSFENIIAVDGGYKFLKKFVD
                     TEPDTLIGDFDSIDRDSAMLKDFNGEIYEFPSDKDKTDGELSIDYCVKNGLNKISFIG
                     ATGGRIDQQFGNLLLLEYALNSDIDAKIIEPGLEVGLIDNKRIIDNKKGNTLSLFSIT
                     DQVLIKKLKGCKYELDNYWLERASSRGLSNIINANKAVIEVDLGKLLYFVCEKC"
     gene            complement(46366..47013)
                     /gene="rpe"
                     /locus_tag="VKN64_03205"
     CDS             complement(46366..47013)
                     /gene="rpe"
                     /locus_tag="VKN64_03205"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMA59276.1"
                     /translation="MVNFAPSILASDFSKLKKEVESVKDAKYLHLDIMDGRFVPNITY
                     GPLVVKAIRPYSDNIFDTHLMIVEPEKYIKDFAEAGSDIITVHVEATDHVHRAIQMIK
                     NEGCQAGVTLNPATPLSTIENIIDDVDLVLIMSVNPGFGGQKFIPQMLDKVKRLGKMI
                     DKSNTETLIEIDGGINFDNVKEVVEAGVDIIVSGSTIFKGNPAQNLKKFRRILKG"
     gene            complement(47006..47869)
                     /gene="rsgA"
                     /locus_tag="VKN64_03210"
     CDS             complement(47006..47869)
                     /gene="rsgA"
                     /locus_tag="VKN64_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMA59277.1"
                     /translation="MEGIITKTLGGFFFVKTNNDVYKCRIRGKIQKKVYPGDVAEINL
                     EDKIVESVKERGNLLMRPKVANVEQVIVLHSLKKPPFDKKLLDRFILMVESSELKPLV
                     VFNKVDLVDNLEEVKEELQIYKNIGYDVFYISAKKHLGLNELYKKLENNISVIAGPSG
                     SGKSTLINSIVEDIDLPTREVSKKLNRGKHTTKHIELITLPSRGFVADTPGFSSIEIE
                     NIDSSDLKYYFPEFEEYENSCKFSTCSHTHEPGCKVKEALEAGFISQERYNSYKDIYK
                     ELQQKEDDLYG"
     gene            complement(47884..49839)
                     /gene="pknB"
                     /locus_tag="VKN64_03215"
     CDS             complement(47884..49839)
                     /gene="pknB"
                     /locus_tag="VKN64_03215"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HMA59278.1"
                     /translation="MIGKLLNDRYKIKKQRGSGGMALVYEAQDILLDRKVAIKMLRPE
                     FVSDEDFINKFRHEAKAVARITHPNVVSIYDIVESEESLYLVMEYIEGKDLKSLIKKR
                     GQLSVVEALDIANQVTAGVEVAHENNIVHCDIKPHNILITDNNQVKVTDFGIARAVSS
                     STMTITDTIMGSAHYFSPEQAQGKEINTYSDIYSIGVVLYEMLSGRVPFKGESPISVA
                     LKHIQKEPKELKEVFPSVPEEVNELVMKTLSKEPEDRQESASQLREEIIMAIKNIEAS
                     DQFKEEKTGEEGSQTKILNKSDIKKEGLEKEKEQDKKVNSKTSKTDKSFFTPEKKKGF
                     KLNKWVIWALVVVILASASVFGLVYFFKVYTEVPIVKVPNLVEKEFSEAEDIAAQQGL
                     NLEKSEEAVFHNDIEEGYIVTQEPKPGERIKQTRNVVVTVSKGPKIVELPDFNNRPLR
                     EAEVILDNLGLSLQEVSYEYSEQYRENYIISQSPEPGSETEINTQVELVVSKGPQPIM
                     VTMPSLLGVDKERAFNLIQRNGLKVGKLNYGESTRYEEGLIMNQQYEAGENVPENSTV
                     DLGISTGLVNKGDFQEHKTNIGINIVGSNNQEIKIVVKDSNGEEVAYQKTHSPGEYVN
                     ETIYSVGPTVLEIYRDGELIKSKEIGA"
     gene            complement(49857..50567)
                     /locus_tag="VKN64_03220"
     CDS             complement(49857..50567)
                     /locus_tag="VKN64_03220"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HMA59279.1"
                     /translation="MSFHIFTNIGNNRQKNEDSYLILQPEEKTAVLAVADGMGGHNAG
                     EVASEIAIESIRDYTFEFDQEIIPQIKSVITEANQNIRDCSQDYENYQDMGTTLTLGI
                     LKDNQLYYGHVGDSRIYLFNGDLNQITTDHSLVNDLIENKSISPDEAFDHPKKNIITQ
                     ALGIDKDLEIQTDKVDISKEDIIMLCTDGLSDMIRFENIQEIFKKDQSIKEMIEELGS
                     KALTNGGTDNITIIMGKL"
     gene            complement(50570..51628)
                     /gene="rlmN"
                     /locus_tag="VKN64_03225"
     CDS             complement(50570..51628)
                     /gene="rlmN"
                     /locus_tag="VKN64_03225"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMA59280.1"
                     /translation="MNKKHILSFDLNGLKKEFQQSDIASYRAEQVFNWIYQNGVLDFD
                     KMSNLPVDLIKKLDSKYTISKFSLDKKLIAEDGTQKYLWKTADDKLVESVFLPYYDEN
                     RYSICVSSQIGCSIGCKFCATGIGGLKRDLNAGEIIEQILKIQIDISEEQYKDTPVTN
                     IVFMGMGEPFLNYDNLNKAVSILNDKKGLNIGMRKMTISTSGIIDKIKRLADENQQIG
                     LAISLNAPNDELRDYLMPINKKYDLDKLMESVQYYIDKTNRRVTFEYVLMKGINDKKV
                     HAEQLVSLLKNKLVHVNLIPVNPVPELNIERPNKDIINYFKDNLEKNNIPVTIRIEKG
                     TNIKAACGQLRASYQNGG"
     gene            complement(51621..52307)
                     /locus_tag="VKN64_03230"
     CDS             complement(51621..52307)
                     /locus_tag="VKN64_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMA59281.1"
                     /translation="MFFYDPTILMLIPVLLIAMYAQYKVKSTFNKYSEINTNVNRTAA
                     DIVSELLAKNGIKDVSIKRIQGELSDHYDPRNKVLNLSQSIYNSKSIAAIGVAAHEAG
                     HAIQDAKGYTPLQIRAKIVPAASIGSNWGLPMAIIGFFLQSQFMIALGFIMFLGAFVF
                     HLVTLPVEFNASNRALTMLKKNNILNQKELKGAKKVLRAAAFTYVSATLVALVNLLRI
                     VVLFGMGSDE"
     gene            complement(52314..53261)
                     /gene="fmt"
                     /locus_tag="VKN64_03235"
     CDS             complement(52314..53261)
                     /gene="fmt"
                     /locus_tag="VKN64_03235"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMA59282.1"
                     /translation="MKIIFTGTPDFSVPSLKELVKEDKINIEAVITQPDKRRGRGKKV
                     RYTPVKKEALKHDIEVLQPANINDDDFIKKLNKRDIDLMVVVAYGQILSKELLDLPRA
                     GCINLHASLLPEYRGSSPIQQAIMDGKDTTGVTTMFMDEGLDTGDIIYQREVAIKEEY
                     NAGDLHDKLAEVGAKLLRETVVKLKEGSVPRLSQTEDKASYTGQLSKEDGLIDWQKSS
                     QDLYYFVKAMNPWPGSFTYLDGKLLKIFEVKPVDDNTDKKTGTVIKAEPEEGLYIQTG
                     KGLLKIEKLQLAGKKRMSTPDFLRGYEIEEGTRLESKKE"
     gene            complement(53271..53741)
                     /gene="def"
                     /locus_tag="VKN64_03240"
     CDS             complement(53271..53741)
                     /gene="def"
                     /locus_tag="VKN64_03240"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMA59283.1"
                     /translation="MAVLPIREIGDPVLRSRAKEVEEITDKTRELIDNMIDSMEAAEG
                     IGLAAPQIGVLQRVIIVEIEEDLKVTVNPVAVKKKGEAVAEEGCLSVPGKTGLVKRAE
                     EVTIKGLNEQGDEVKYELEGLKARAFLHEIDHLNGILFVDKALEVKDEEPEMVE"
     gene            complement(53763..55997)
                     /gene="priA"
                     /locus_tag="VKN64_03245"
     CDS             complement(53763..55997)
                     /gene="priA"
                     /locus_tag="VKN64_03245"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMA59284.1"
                     /translation="MNKFAEVIVDLPVPKFDKIYDYVVPEELVDKLKIGMAVKVRFGK
                     RKIRAFILNIKDETEVEKEKLKEIDSLITDEIFFKESDLKLYKWICEFYCALLISVIK
                     AAVPTAVFKGKISKKTVKAVRLKLEKKQIDKKLKELENRAPSQFKVLNYFKENENEEI
                     EVKRLLDKLDVYRGAINYLVKKDYLEYTENIERRIPYKDYDNELKAELQPTKAQAEVL
                     QTINNNLFEPKSNTYLLHGVTGSGKTEVYMQLIKEVLQAGQGAILLVPEISLTPLMVK
                     RFYSRFGEQIAVLHSALSSGERYDEWRNIKRGKAKIVIGARSAIFAPVKDLGLIIIDE
                     EHENSYKQGNHPYYHAREVAQKRAEINNIDLVLGSATPSLESYYKAKEGDYTYLSLPV
                     RIDNKEMPPVEVIDMTEELKKGNPSIFSSKLEDGLKQTLSKGEQAILFLNRRGYSSFV
                     LCRSCGEVIKCENCDISMTYHQGKDKLICHYCGATRDIPKYCPNCSSKYIKDFGIGTE
                     RIEKEVKKLLPEAEVARMDHDTTTRKGSHRRILNKLENNEIDILIGTQMVAKGHDYPN
                     ISLVGVITADTMMNLPDFRSSERTFQLLTQVAGRTGRGKKKGVVAIQTYNPDHYSILA
                     AKEHDYQYFYKKEIGLRKALQYPPFTNLVNITINHKKKNKAEKAAKKLYNHIKDYKIK
                     KIIGPSPAPIERIRGRYRVQLLLKFDDLDNRTSILKKLKDEFLKNISMNVKYNIDVDP
                     ISML"
     gene            complement(55990..57039)
                     /locus_tag="VKN64_03250"
     CDS             complement(55990..57039)
                     /locus_tag="VKN64_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMA59285.1"
                     /translation="MQTQESRIDRQTVFKGLRMAVVLSLLISAIIIVITIDEDTLERV
                     LKSMDMKYLWIIMGLILINIASAGQRLRVMISSVDKKLSLKDCMIIHISGAFVSNVTP
                     FASGGGPFQIYFLHKKGVNVGKASTAVVTNFLLRLFYFGILTPIFLIFFNEYISAGVL
                     PDYIFYLAFGMGVLISVGIILLTLIPKIGDKLTDYVLNINKINNFIKKSYKAKRWLVK
                     SRRELRDFRRSFGILRKNKKSLFWGAFYTIVFWSSMFMIMPVVLMSFGAEPHFLQAYI
                     MQTIFYLMLPYSPTPGASGVAEIGFASLFVAFIPSDIVGLVTFGWRLFTFYTILVIGG
                     IFALREISKRRNNNE"
     gene            complement(57103..58278)
                     /gene="metK"
                     /locus_tag="VKN64_03255"
     CDS             complement(57103..58278)
                     /gene="metK"
                     /locus_tag="VKN64_03255"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMA59286.1"
                     /translation="MAKNYLMTSESVTEGHPDKVADKISDSILDEILKNDKDARVACE
                     TFVTTGLAFISGEITTDCYVDVTDITRQTIMDIGYDRAKYGFDGETCGVITSIKEQSA
                     DIALGVNHSIESKEGRDEEQIGAGDQGIMYGYATNETEEYMPLPITLAHKLAQRLALV
                     RKKDIVDYLRPDGKTQVTIEYEDDKPKRVDKVLISAQHHPEVAQETIKRDLCEFVIKK
                     IIPADMLDKQTEILINPTGRFVIGGPHGDAGLTGRKIIVDTYGGTARHGGGAFSGKDP
                     TKVDRSASYAARYIAKNIVAAGLAEKCEVQLSYAIGVAHPLSVMVETFNTSKVSEDRL
                     VKVIKDNFVLTPGGIIDKFDLMRPIYSQITNYGHFGRLDLELPWEELDKVEIIKNKV"
     gene            complement(58349..59695)
                     /locus_tag="VKN64_03260"
     CDS             complement(58349..59695)
                     /locus_tag="VKN64_03260"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMA59287.1"
                     /translation="MELNIFMKEILKRAEQNEFDNSEIYLNKSENFEFRLYKGEVDYY
                     EENEEYGVAFRGEYQDKMGYSYTERIDQSIIDTVLKNAKSNAEIMDEEEEEIFAGDKK
                     YKELPALTGIYEFDREKKIELLKTIEKKIYDLDDRIRNINYCIYADEKTGEHILNNKD
                     LELTYENDIAYIYISIIAEEKGEVHTSTHYKLFRDIKEVDPDKFAQKVVKDAVSQFGA
                     ETVKSGSYPIILRNEVFIDILSTFSSTFSAENVQKGLSLFKDKLGEKVAAAKVNIVDD
                     PFYEKGYRRTPFDSEGAAAKYKEVIKDGELTTYLHNLKTAKKSNTETTGNAYRSSHKS
                     KISISPTNMYLQKGEYTFKQLLKEMKEGLVIEDVQGLHSGANQVSGDFSLSARGFYVQ
                     DGNIDKPVEQITISGNFLQLLKDIKLIGDDFKMGLPSSGHIGSASVLIKSLDISGS"
     gene            complement(59695..>60900)
                     /locus_tag="VKN64_03265"
     CDS             complement(59695..>60900)
                     /locus_tag="VKN64_03265"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMA59288.1"
                     /translation="EIYVYTNKDKKDNLLKLAKKAASMFDKTKKDNINLKYVDYKNLH
                     PITIMPADVDQIEKVNFIKKAYRAADGYDELITQVVVGLTDYTQNVQIANSEGVFVED
                     ERTRVRLGIQAVATLQDEKQTGYLGPGGHMGFEFFDEINVEKQAEDAARMAVTMIKAG
                     HAPQGKMPVIIDNKFGGVIFHEACGHGLEATSVAKNNSVFANKLGEKIASEKVTAIDD
                     GTLPNAWGSQNFDDEGHTTQRNVLIEKGVLKGYMIDYLNGRRLEMEPTGSGRRQSFRY
                     SPTSRMTNTFIDNGGHSLEDIISQTEYGLYAKHMGGGSVNTATGEFNFNVREGYIVKD
                     GSIEKPVRGATLIGKGADILKKIDLVGNNLDLAEGMCGSLSGSVPAGVGQPTIRVSEL
                     TVGGRGGNE"
BASE COUNT        17759 a        12749 c         8455 g        21937 t
ORIGIN      
        1 ctttttactc caaaacaaaa gccagcctca tccgcagtta ttatctccag caccttcacc
       61 tccttccaga acaacaaaac aagacctgta caacaacctc caaaacaact ttgttttttc
      121 aaataagcgt tatgttactt gctcatagat atttataaaa taacatattt ttttcaaaaa
      181 ggcaaataat tattcaattg aatcaagttc ttctttgatt tttttcatga ttatttcgcc
      241 cacttctgac ttttcttccc gtgaaagttt tttatcataa tattgatcta aagaaaatgg
      301 ttcccccacc gatactttta aggaagaatc atcatctgaa tgtttaattc cgaccggaag
      361 aataggactt tcactcatta gtgcaattaa agtaacacct ggtttagcat cacctaattt
      421 atcacctttt atccgggtgc cttctgggaa catacaaagt accttcccgt tttttaatat
      481 ctctaaagaa cggcggattg cttttttatc cggagttcct cttttaacag ggaaactccc
      541 gatttgatgt aagataaagc cgaaccattt tttatcaaaa agctcctgtt tggccataaa
      601 atgtagttgt ctatttacag cacatcctac tatgggagga tctaaataac ttacatggtt
      661 tgatactact attaaaggtc cttcttttgg gacattattt cttcctttca cccgccagtg
      721 gtgtactatt ttaaaatata ggttaagtaa tacataacta acccaataga ttacttttcc
      781 catcctcact actcccctct tataatacgt aatatttttt ttaaaacctg ctctttattt
      841 agatctgttg tatctattac aaccgcatcc tcagcctttt taagcggaga atactttcgg
      901 ttagaatcta ttctatcccg tcttattatc tcttgttgga cttcctcaaa agacttatct
      961 tcacctctat taaccacgtc tttatacctt cgtttggccc tttccttgac agtagctgtt
     1021 atataaaact tataatcagc ctgaggtaat actctggttc caatatctct cccgtccatt
     1081 actatatcct tggtttgggc aatcattctt tgttttttaa caagttcatc ccggacagct
     1141 tttatcttag ctacggctga aacattatta tctacttttt ctgtcctaat ttcatttgta
     1201 atatcttggt catccatgta tattttaaaa ttattatttt tgtattcaat atcaatactt
     1261 agatctttta ccagtctttt taacttctct ttattattaa catccacacc atcctctaag
     1321 gccgctaaag tcacagccct atacatggca ccactatcta aatatgtaaa gtttaacttt
     1381 tcggccagct ttttagctac agtactcttc cctgccccgg ctggaccatc aatagccact
     1441 gaagttgtca atttaccacc tcatttattt taatataata ttttagctaa tttgtcaaaa
     1501 aatcccggaa aagaattctt aatacaagag gtatccatca acccaatttc atactatgtt
     1561 attaacctta agactgtaaa tatcatggcc atcctatggt ccattttagg gtctaaaaca
     1621 ccactttcct ctatcttttg tggtcctctt atttcaaaat catctttgca agttttcata
     1681 tttaaattta aaatatcaaa agcactcatt aaaacaaaca acctatcact ttcttttatt
     1741 cttaatacct caattaaact tactgttatc ttccggctgc tttaagcgct gcaacaacaa
     1801 ttaaaggtat ttcatcaatc atagttggta ttaaatctta gtttacattt aaccctgcta
     1861 aatcaccgtg cttaataact atatcaacta ttaataccta tttacctact ttttattaat
     1921 taccggttct ctttgttttc tttttaatat ttttcactaa ctttttgaaa ttacttaagt
     1981 tcagggattg ataaccatca cttaaagcca gatggggttc aggatgaatt tcaaccatta
     2041 ctccctgggc tcccagagtt aaagcagcag ctgcggctgg ctgaattaaa tcccacctac
     2101 cggttccatg gctgggatct actataatcg ggtaatcgct taattttttt aagataggga
     2161 ctgccatcag atcaagtgta aatcttgtct tctcgacaaa ggtccttatc cccctctcac
     2221 agaggattat attcttattc ccctgcctat agatgtattc agctgccatt acgaactctt
     2281 cgtaggtagc actcatgcct ctttttaaaa gaactggttt atctgttttc ccgatttctt
     2341 taagtaaact gtagttatac atgtttctag atccgatttg taagatatct gaatactcac
     2401 ttactaccgg tattttatct gtatccatta cctctgtaac tatcttaaaa ccgtgttttt
     2461 gggcctcgtc ttttagtagc tgtagtcctt tctcttctaa tccctggaat tgataggggg
     2521 aagtgcgggg tttaaaggcc cctcccctta tatattcaat atttaaatca gaaaggattt
     2581 gacctatttc ttccatctga actttatttt ctaccgaaca gggacccgcg ataatttcaa
     2641 agtcggcctg ttgaaaggct tttttacctt caatttcaat aaatcgggtt tcgtctagaa
     2701 actgatgtgc ttcatcttcc tctaatatat tatcaggttt ataggttgtc cttattatat
     2761 ttacaaactt tttactaata tcagaaatta ttattatggc aataaggtca tctctttttc
     2821 tttttaaata attaatatct tcatccttca gaaaatccac gaaatttata taattttcat
     2881 ttttcccttc atatattata atcataggtt tggctcctct gccatataat taagatcttt
     2941 tcttaaattt ttagcctttt tcagataaat atgacgaata ttctctttgt ctttatcatc
     3001 atttataaaa atcataactc ttatacattt ttcaagtgaa ccttcaacat acatctcctg
     3061 tagacacata atagaggttc tttgaaaccc caacttcctt acccccctgg ccgggataaa
     3121 tagagtccag atcttcggtt gtggaaaaga taaaacttat tatatcttct ttttgtagat
     3181 tatttacttt aactattttc tttattaaat ccttaactgc gtttaagatc tcatcttctt
     3241 gattgttttc tactgtaatt gcacctctta tagtatacac gtctaatccc cctctttcaa
     3301 taaaaataca ccctcaaaca gggtgtatgt taggattact tatctaattg ctttagagca
     3361 tgcttagctg cttcttgttc agcttctttc ttgcttttac cctttccaaa accgagttct
     3421 ctctcttcaa aatttacaac gacataaaat tctttattat ggtcaggccc tttttctttg
     3481 acaacttcat attcgggtct attattacta tttttttgga ttacttcctg caacatcgtt
     3541 ttatagtcct gaatatagtt accgttatct acttcatata taaatgattt aaaattgcta
     3601 atgataaatt caaaggcaaa atcaaaacct ttatccagat aaatagctcc taaaatggct
     3661 tccatggtat ctgctaaaat tgaatcccgg tttctacccc cggataattc ttccccttta
     3721 ccaagcagta aaaacttgcc taaattcaat tctgaagctt tttcagctaa aaccggagcg
     3781 ctaactataa ccgaccttat cttggccagt tttccttctt tataatcatc atatttatta
     3841 aaaatataag tactgatcga taaacttaaa actgagtctc ccaaaaactc taatctttca
     3901 ttatccttga tctttagttc ggcattctca ttcgcaaatg acttatgggt taaagccctg
     3961 cgaagtaatt tcttattact gaatttaata tttaatttag actctaattc taccagcttg
     4021 tcaactgaat aatattctgt catctaagtt tcaccacacc tgtcatcatt atttcttttc
     4081 aaaaaggggt ataatacata tccattatta tacccctttt aattttatat ttaaagttta
     4141 aagatttccc tctatataat caactgcatc agatacagtt gcgatctctt ccgcgtcttc
     4201 atcaggaatt tcaatatcaa attcctcttc caatgccata attaattcta caacatctaa
     4261 tgaatcagca ccaagatcat caataaatga ggcatcttca gtgatttcat cttcagaaat
     4321 tgcaagctct tcagatacta ccttcactat tctatcaaat acatctgcca tcatttttca
     4381 ccccctttca cattaacatt cctccatcga tatttatagt ttgtccattt acgtaattag
     4441 ctaatggaga aaccaaaaac attattacat ttgcaacatc ttctacttca ccaaatctat
     4501 ctaagggaat attttttaac atatcttctt ttactttagc aggaagttct tctgtcatat
     4561 cagtgttaat ataaccgggg gcgactgcat ttacattaat accccttttt gacacttctc
     4621 tggccactga cttagtaaaa ccgactatcc ccgctttaga agctgaatag ttagcctgtc
     4681 cggcattacc cattaatcca actacagaac ttaaattcac tattttacca tggtgttgtt
     4741 tcatcatgta acggattgcc tgttttgtac agttgaaagt accctgtaaa ttaacattaa
     4801 taacatcatt aaaatctttt tccttcattc ttaaaaggag tccgtctttt gtgataccgg
     4861 cattattgac tagaatatca attttatcga attctttgac agttgcttta attaaatttt
     4921 ttgcgtcatc catctcagcc acattacctt gaattactaa agcttgtctg cctaaatctc
     4981 tgatcttttg ggctgtctct tctgcgcttt ctttatcatt attatagttt acaacaacat
     5041 ctgccccttc ttcagccagt ttgatagcag tagctgcccc aataccgcgg gaactaccgg
     5101 ttacaacagc aacctggtct ttgagtaaca agctatcatc tcctcattat tcgacatata
     5161 gagaactaac ataaaaattc aagttgtcta gataaagtcc tgtaaacttt tcaattaatt
     5221 catttagttt tacctgcagg gaatttatgt attcaactat ctctacacca tattttaata
     5281 taatttcgat gttaacacaa atctcattat cttctttttc aatatggatt gaatttactt
     5341 ctattataat cttttctttg gaaagttcaa aagaaatcaa atcccgaatc acatggttat
     5401 atattacaag gttaccaaaa taagaaaatt tcggtctcac aattgtcttt tcttttttta
     5461 tagatccctt acctttgcca aaaaacagtt caagggaatg aataaaatag ccggggaatc
     5521 ttgattttac ttcaatcttg ggaaccggta tcacatgctt accttcattt tttctggtat
     5581 caattgcttt tttaatttca tcttcttttg caatatcatt aattttaata atttcatcta
     5641 ttccatctaa tttcaaattg gcaacaattc tttctatcat ccgcattgaa gtacctaaga
     5701 ttaatatttt attttcatct acatccttta gggcttcttt tatttcattt agatgatctt
     5761 cctccagtag gatggcagtc cgaatcgcct ggatctttct tttatccctt ttagctgatt
     5821 tacctgctaa tactcgtcct ttatagatta gaaggccgtc atccagtatt agagggatat
     5881 catatttatc ggcaacaaaa gccgctctat ggctttttcc tgagccgctg acacctacca
     5941 gtgcatatac atacaatatc tacacccctt aaaaaatatt cttaaattta ctataaatga
     6001 aatttattaa aatttcaagt gattcattta aaaagataat aacttattgt ggttattctt
     6061 ctcaattttt agtatttttc cagattaagt atttataatt aataaccaaa tttttcttat
     6121 tattttacta ataaaaatta atttttacat tataggttgt tcaaaataaa ataagccccc
     6181 taaataagtt taaaaacatt acttaggggg ctattttaaa taaatattta gtcattatct
     6241 tttttatatg gtattctttc ataaggttcg aacttacttt ttgcttcctt agttaacccg
     6301 tcatatacac ttagatttct atcttctaga tggtcaaaga tcataggtga tttaacccca
     6361 taagaaacat gttttatggg atcactgtct aaacgggcgg agacaatttc ttctgttccc
     6421 cgagctaagg ctattttttc agcaatagga ctaataatca tgctgtgacc gaaataataa
     6481 ttttcttctg catctgctcc aactgaatta acaccgatca tgtagatatg attatcataa
     6541 gcacgagctt tattggtcat ttcccaaata tcataacttc tcagtaaggc agaaggccta
     6601 acaataactt cagccccttt gatagctaag attctgctta attcggggaa gtctccctca
     6661 taacaaattg taattccaat attggccagg tcagtttcaa aaacctctac tttgttacct
     6721 ggtgttgtcc agcctccccc ctgtaatctt tcggagggga aaaggtgtgt tttggaatat
     6781 tttccaatta tattaccatc aggaccaatc aagaccgcac tattataaac aactccccgc
     6841 ttttcacctc gttcataggt aggtaaaaca acaaatacac tatttttttt ggctgctttt
     6901 gcgattggac ctgttaattt tccgggtaca taatcaattt tatcccacag ttcttctgga
     6961 ctacagccag gagcaaatcc ggttgttatc gactcgggaa aaacaattag ttcagcatta
     7021 tgctcatcaa ctgccttatt taaccaggtt attatcttat ttatattata ttcaatatta
     7081 tcaggtttaa cactaatctg aacccctgca acaacaaaat ctttcaagct aacaccccct
     7141 agcctaaata ttttttgatt atctcttcag tgtgttctcc taacttagga gccctttcac
     7201 gttcagtctc atcttgagta tctgatagtt taattggatt accagcaatt gtaaatctgc
     7261 catcgtcagg gtctacaaca tcaaccaaca tattacgggc ttcaacctga ggatgattaa
     7321 ataggtcttc tacatcattg ataggtccag caggaactcc gtgattatca agtagttcaa
     7381 ttacatcatc agccttttta tctattacaa aatcattaat tatttctact aactcttccc
     7441 tattttcagt ccgtttatcg tttgttttaa aacgaggatc gtcaataaga tcttctctac
     7501 caattgcctc gcaaaattta gcccatatat tatcattacc aacagcaata ataaaccatt
     7561 catcctttgc tttaaatgcc tgaaatggtg taattgaagg atggcggtta cctaacggtc
     7621 ccggggcctc ccctgaagct gtataacggg caatagcatt ttctaaaatc gcaatctgac
     7681 tatctaacat cgcaatatcc acttttttac ctttgccagt tttttttctt tcatgtacag
     7741 ctgccagtat tcctactgtg gcaaaaagag ctgctacaat atcaccgata gaggctccta
     7801 cccttaccgg gggacgatct ggttctcccg ttatactcat gattccacca gcagcctgga
     7861 ctatcatatc ataagctgct ttatctgaat aaggaccact gtgtccaaag cctgaaattg
     7921 cagcatatat taatttttca ttgtattctt ttaaggtatt ataatctaag tttaactttt
     7981 ccattgttgt aggtctaaag ttttcaacta atatatctac ttttttgact aatcctaaga
     8041 atgtattcct gtcttcttca tcttttaaat taagggtaat acttttctta ccccaattta
     8101 gactaatgaa ataagcactt cggccttcaa taaagggtcc aaaatgtctt gaatcatcgc
     8161 ctgtttccgg tctttcaact ttaattattt cagcccctaa atcctttaag agcatagtac
     8221 aaaagggacc tgcaagtact ctagataaat ctaggacaac taagtcttct aaaggttttt
     8281 tattattaat gataatcacc ctcctattta ttttcttttt taatgaacat attttatata
     8341 ataattaata caggtctggt ctaacgatag ataataaaaa gtctatatat tttcaatata
     8401 accaatttga tcaaattaat caatttttgc ttagtattat aaatcaatat tactctatct
     8461 atagaaaata agcaaagacc tcctctttat ttttttgtta tctatcagag acctttaact
     8521 tatttccttt attttttaaa actaaagtat ccaatattat ctttgattat ttattttaac
     8581 tattttactt ctctttctca atataatcat aaattctttc cggaataacc ggcatttctt
     8641 tgatatggat cccaattgca tcctgaacag cattacctac tgctgaagga atagaaagca
     8701 tgggatgttc tgctacacca cgagctccat agggtccgtc ttcttgagca ttttctatta
     8761 taattggttt taattccggt atatcagcaa ttgaaggtac tttataatca gtaaaagtat
     8821 ttgtcaagag tctaccgtca tcactaaatt gatatccttc catcacagcg gaaccatagc
     8881 cttgaattac tccaccttca atttggccaa ttacattctg cttatttata gttttaccta
     8941 catcgaaagc agaagcgatc ttaattactt ccagttcacc tgtttcttca tcaacaatta
     9001 cttctacacc atgggctcca taagtccagt caagggcagg taatccctgg cctgtttctg
     9061 gatcaatgtt agttaagcct tgagcaatat agttacctct tgcaactaaa ggcccaccga
     9121 ttgcattacc atttggataa gaataaccca ttgctaaatc tttaaagcta atatacttat
     9181 ctttatgatg tctgattttt accttttcat cttctataat taagtcttct acagaacatc
     9241 ttaagggctc agaagcaact tcttttaact gttgagccat atcctgacat gctttgatgg
     9301 tagcattacc ggacataaat agacccttag aggcaacagt ttgccaatca taaggttgag
     9361 tttctgtatt ttgttcccag actacattta ctttatcaat tggtatatca agctgatctg
     9421 atacaatctg tgccaaagca gtataagtac cttgaccgta atcagttaaa gaaacagtta
     9481 agttaagagt accattttca ttaaacttaa gaaaggctgc agtccctgta tcggtaggca
     9541 ttgcaggtgc cttgtgtaat aaagcaagtc ctttacctct ttttttgccg tccggcagat
     9601 catcagaact tctattttca taatctattt cttctgcaac agcttccaga cattctagca
     9661 ctttaccatg tgattcacca atatgttcac cagtaattgt gactgaacct ctctttaagc
     9721 catttttcat tctaatatca taagggctca ttcctatttc ttcagctaca aggtccatct
     9781 gtctttcaac tgcccagaga atctctaagt ggccaaaacc tctataagca gtaccaaaaa
     9841 tcttattggt atagactgtt tttgaatcta gttttacatt atcaacttca taaggaccag
     9901 caccagcagt tgctgctgct ctagtaacat taacaccata atcagcgtta gcaccactat
     9961 cccataaata ttcagattca aaagcaacaa tttttccttc ttttgttact cctgttttca
    10021 atttggaata taaaccttgt ctaactggaa cagaattaaa ctcttccatt ctggacatta
    10081 caatcttaac aggtcttccc tgagaagccc ttgatagaca ggttactaaa ggttcaaaat
    10141 ggatacctgc ttttccaccg aaacctcccc caacatatgg gatttgtact tcgatatctt
    10201 catggtttaa atgaagtcca tttgctaata aatttctaac tgcaaaagga gattgagcag
    10261 atgtccaaat tttaactttc ttaccaggca tccacttcgc tattgaagta tgggtttcca
    10321 aggcagtatg atcaaccatt ggtaattcaa attcattttc tatgatataa tctgcttctt
    10381 cgaaaccttt ctcaatatca ccttttctga tcttagtatg gtgtgcaata ttggttcccg
    10441 gttttggaaa aaagacaggc gcactttcta attctcctaa attttcatga actaatggcg
    10501 cgtcttcttt aacagcttct ttgacatcta atgctgcctc taatttttca tattcaatat
    10561 ctataagttc aatagctttt tctgcaattt cttctgtttc agctgcaact gcaacaactg
    10621 gttcaccttg atacctcacc ttgtcgacag ctaatatgtc tttatctacc aggtaaagcc
    10681 ctactttata gggaaggtct ttcccggtca gtactgtcca gactcctggt aatttttctg
    10741 cttctgagga atctatatta actattttag catgggcatg agggcttaac ttcattttgg
    10801 catgcagcat accctgtaat tccatgtcat gaacaaaggt tgcactaccc gtaacttttt
    10861 ctaatgcttt aattctattt acacttttac caatcatctc ttcactcact attagttgcc
    10921 ccccttcttc tcagctgcac gttttaccgc atttctaata gagtaatatc ctgtacagcg
    10981 acaaatatta cctgataaag caatatcaat atcttcatca gaaggattag gatttctatc
    11041 taataatgcc tttgtagaca taaccatacc tggtgtacaa aaaccacatt gaatagcatt
    11101 ctcttcaata aaagcttttt gtatatcgct gatctcttca ccgatcgact gtccttcaat
    11161 agtttctatt tcagaaccat ccgcttctac agataaaatt aaacaacttg ttacagcctt
    11221 accatccatg ataacagtac aggcgccaca ttctccttct ccacaacctt cttttgcacc
    11281 agtaaaattt aatttttttc ttagggtatc aagaagaatt tcattttcct gtacttctaa
    11341 ttcaaaaatt cttccattta ctgttagatt aatggttttc attatttatc acctgcttcc
    11401 cataaactgg aaagaattct ttttgtataa acttcagata gcttttttct atattttttt
    11461 gtacttctta catcgtcaat cggattaatg ttatctaaaa ttctgttaac aattttcccc
    11521 tgcttttctt caaaagagcc atctgcaaat aaaagatcac taacatctac ataaactggg
    11581 gttggagcta ctgcgcctaa agcaaatgca agatattctt ctgctttaga tagataccct
    11641 gctaccccta ttccacttaa atcaggacct ttagttcttc tttttttgag ataagctccc
    11701 ttgccatctt cagcatgttt ttgtactatt aagcctttga ctacttcttt atcttttaat
    11761 acattgcttt ttactcctgt cgcaaactca tctaaaggaa cctctctact tccctcttta
    11821 ctttcaatta aaacggtagt ctttagtact aataagctgg gaaacatatc tgcacccggg
    11881 gaagtagtac ataagttgcc aacaactgtt gctctattac gaacctggtg agctgcaact
    11941 tctaaagctg catctgctaa tattttataa tcattattaa ccagttcact gtcgattatt
    12001 tcattacaag ttacagttcc acctataaat aaaccagcat ctctttcttc tattttacca
    12061 aaatctttaa tacctttaac atcaataata taatcaggtt ttcttgtctt atgtttcata
    12121 tcaacaaaaa ggtctgttcc acccgctaac aaggcggatt cgccctcatt attatctaaa
    12181 atttcaaata attcttctaa ttttgttggt ttaaaataat caaatttatt taacattaat
    12241 atttttcacc tccaacgaat tccaaaacta cttggtaatt ctggtaccag cattaccatg
    12301 aacggcttct aacacttttt ctatagaagt tattatagcc atttctccgc cgctttctac
    12361 aaaatcacaa gcagcctgta cttttggttt catacttcct tcaccaaaat gacctttttc
    12421 aatatgttct tccatttcat taactttaat ttctccgatt gcttcttcgt cttttttacc
    12481 ataattaata tatgcatttg aaacatcagt taaaactaag aaaatatccg aatctatttc
    12541 ttcagctaaa agacagcctg ctctatcttt atcaattact gcctctacac catgtatctt
    12601 attatcttta ttaacaactg gaattcctcc cccacctgat gcaataacaa tctctccatt
    12661 tcttacaaga tcattaatta tgggagcttc aataattttc tcagggatgg gtgaaggtac
    12721 aacttttctc cagcctctgc cactgtcttc aatccaatct tctccctttt cttttaccat
    12781 cttatcagca tgttctttgt cataaaaagg tccaacaggt ttggtcgggt tatcaaaagc
    12841 actatcttct ttattaacta aaacttgagt tactacagtt gctacatttc tatctaaacc
    12901 taattcagca aaaacattat gcaaactatt ttgaagcata taaccgataa atccttgagt
    12961 ctgagagcca caaacatcaa gaggcatagc tggaacagaa tcttttgcct tatcctgttg
    13021 aattaaaata tttcccactt gaggaccatt accatgtgtt attactactt catgaccatc
    13081 attaactagt tgaccgatct gtttactggt agataaaaca ttttcaaact gttcttttgc
    13141 agttccatcc tgacctggtt gtaatattgc attcccacct aatgcgatag ttaatttcat
    13201 ttatattcct ccatttattg tatttattta aaagtaaaga aaggaaccct gtggtaagca
    13261 ggattccatc ttattactta tatttactct tcttctacaa aagcaactgc gcgtccaata
    13321 ctagattcta aatctacacc tttccatggg aagaatccta ttttacatct cttattaaga
    13381 attttatcaa tttctccccc aatgttttca gcgtggataa ttccttctgg gaataactta
    13441 gtgtgcatta gctgtctgct ttctggatga gagaatactt catctaatgg tgcaccaagg
    13501 tgttcttcgg cttcttttgc cagatcaggt cttagctttt tacaaactgt attcatcggg
    13561 tgatcagcag aaccacagtc aacaccgatc catttcaact ttttctccat tgcccaatga
    13621 gcaaattcca tgtttggacc agggtgtttc ataaagtatc tttcttcatc tgcctcaggt
    13681 tgatcccaac catatttatg gtaaccagta ttgattatta agacatcgcc ttcttttact
    13741 tctactcttt cttcaatcat ttctgatgta tatatatcat agtcttctac tgcatcgctt
    13801 agatctacta ttactccctc accaaataga tcttctaatg gaatgctggc catatctctt
    13861 ccagcagtcc agaagtgtct ttcaccatct aaatgagtac caatatggtt actagtttta
    13921 ataatttgtc cgtttactct ttcagttgaa agacgcttga agtattttag ttctaaaggt
    13981 tcatatgttg gccatggggg tgtagaaaca cctatttttt gagttaagtc atataatttc
    14041 attaataatc tctcctttta tttaatatat ttttgggcaa aagcccttat tgcctttttg
    14101 aaacactctt caggtgggtc tactaaacct gctccgattt gtccaattcc tggttcttta
    14161 tgagcaatac cggtattaat tgccggtaag attccggttt caattatctt acggaggtcg
    14221 ataccggtcg gagttcctct aaagttaaga ggtggtatag taaagttctc attttcagta
    14281 actgttattt catacatatc tcttgtaaat ttaatagaat cagaaacagt acctcctaca
    14341 aatcctacta tggctggggc agctgccatt gcaaaaccac ctacagcagc agtttcagaa
    14401 attgtagaat ctcctaagtc ccggcaggca tcttcttcag tataacctgg gaaataaagt
    14461 ccatcaactt tttgggcttc accgacaaac caatcatcac ctaagccgct tactctgata
    14521 ccaaagtgta caccatttct agccattgct gttaggactg tactatattc tattccatgt
    14581 gcagcatcca tagtcgcttt ggaggcaggc attgataagt ttaaatagaa atgttcattt
    14641 ccagacataa attctataat ttcttttatt gtttcgttat cataatcagt ttgtaagagg
    14701 tagggtgtaa tttctcttat aaacaaggag gtgcctgctt tatttctatt atgaacttca
    14761 tcacccatgt gaagggcttg agaagttata ttttttaagt ttataccatc ttcagttttg
    14821 cgcaaagcct ctcctatggc tggacctaac actttttcca tccattttaa tttttcgata
    14881 acttccggtc cattggcacc aaatcttaat acttttccta atccttcatt aagtgttgta
    14941 taggctgtgt taccataagt cttattttca actacccaaa caggcatgga gggtgaaaca
    15001 atacctgtca tagggcctac agcattatgg tggtgagcag gatcaaaagt aacttcacca
    15061 gcatctaaca tcgcccgggc agattcttca tcttcagccc aaccttcata aatacaggca
    15121 ccaattgctg caccctgcat tggtccagac atatcttccc atttttcagg aggggcagca
    15181 tgtaaaatca ttttaccttc aaaatttggt aaaacatctt tagcagtttg aacatcaacc
    15241 actacaggtt cacttttttg catccgttca acagcttctt cattcgcttt ttcaatttta
    15301 tcacttattt tttctatatc atctaacaat gcagccattt ctttatctcc acccgcgggt
    15361 ggcttccaat caacctggac tgcatcataa ccttcatctt ttaaattctc agaaaaagat
    15421 tctaatccaa tatttattac tttcaaatct tctttaaata aatcttctag tttcttaccc
    15481 atattatctc tcccccttat ttaagatcac ttaatatttt attcactacg cgcacagcct
    15541 gaacatttgt ggggagtacc ttagctccaa cctcacgtaa tttttcttct tgtgcctgaa
    15601 gattttgagg atctttatca gtgccgcata cagaagcgat aactgataga tagccgccgc
    15661 gttcagtgac tttttcttta gcttctttaa taacttttgc tgcttctcca gctgggtcca
    15721 gatgtgaacc atagcctaat acaatatcta aaagaataac tgctgtttct ttatcatctg
    15781 cttcttttat tagtctctca tttcttaatg agggttcaat cataggatgt ggtttacctc
    15841 ttgtaaattc atcttcacct aaatcaataa ctgtatcttt tatacttttg agatcttcct
    15901 ttaatcccaa ttcttctttt aatggaatat tagaataaat atctccagat ttctcagata
    15961 atagtataac tgcctcactg cataaagttc caccagtata taatcctcta agatattttt
    16021 gttctgaaga cattttatta acttcttctt tggcaatttc agctatttct tcttttgtta
    16081 aaccagcagc agaaacttta tcttcacctt taacaaaggc agctgctttt aaagctgcgt
    16141 cctctagggt gtctgcccaa atagcatcag taccttctat aacagaaggg tcaccaccta
    16201 aaaagttaac aatgaccggt ttagataatt tctttacttc atccattatt tttttactta
    16261 cttcttcagc agggggttta gatataagca cgattacctc agtctcatca tctttatcta
    16321 aagctttcat atcttttatc atttcaacac cttttacctt aaacttaagg tcgcgtccac
    16381 ctgtacctat aacctgagat aatccaaccc cctgtctatc aattaaactt gatacagctt
    16441 gagcgccagt acctgaggca gctacaatac ctactgggcc tttatttaca acattagcaa
    16501 aagctaatgg tgctccattg attatagctg taccacagtc tggtcccata agtaagagat
    16561 ctttcttact agctattttc ttaagttcaa tctcatcttc aacaggtaca ttatcactaa
    16621 acagcattac gtgcagacct tttttcaaag ccttccgtgc ttcacgtgct gcatattcac
    16681 cgggtactga aattattact aagtttgcat ccggttgaac atctaatgca ccatctaaag
    16741 aaccaggctt gtactctttt tctttatctg aagaatcttt ttgttctgct aacatttcat
    16801 tagttttttc aattgctttg tttaatactt cttcttcacc atctatcaca attaccaaat
    16861 catttggctt tgcagattcg gcttcaggta ctaaaagtcc agcggactct aaaatacctt
    16921 tattagcttc agtacccatt actattgttg cttccttaac tccattgata gccttaacat
    16981 ctttggaaat tgacattaag gtaacagaat caaagtaggt accttcttta attaaagctt
    17041 tttttgccat ccttaacaac tcctttatga tttatcattt tttataactc actagttcaa
    17101 tgtaaaaatc atctcttatt taaattacta aatcatatgc ctaaatagtt atacctaata
    17161 aaatatcgaa taaagatttt ttggtactaa tcaataactt cttgcaatat atttagaatt
    17221 cctttgtaat attttacaaa tatttaatat ttcttttgta tgaaatctta agcaaatatt
    17281 gacatcatca caccagctgc cacagcagat ccgatcactc cagccacatt aggacccatg
    17341 gcatgcatca gtaaatagtt ggaagtatct gctttttgac cttctttttg tacaacccga
    17401 gctgccattg gtacagccga tactccagcc gccccaatca tgggattgat cttatcggaa
    17461 gaaaaattat tcataagttt acccagtaaa actcctgtca tggtaccgaa agcaaatgct
    17521 aaaacaccta aaattataat ttgtatagtg tctaacttta aaaatttatc ggcagaggca
    17581 cttgccccga cagctactcc taataatatc gttacaatat ttatcaaaga gttttgagca
    17641 gtctcactta agcgatctac aacaccactt tctttaaata aattaccaag cattaacata
    17701 ccaattaatg gtgccgcatc tggtaataat aatagcacca caattgtgac taaaatagga
    17761 aataatattt tttctgactt tgaaaccggt ctcagctgct gcatctttat ttttctttcc
    17821 tttttactgg tcaatgctct aattattggt ggctgaataa ctggaactaa tgccatataa
    17881 gaataggctg caattgcgat tgcccctaaa tactctggag ctaactgtcc tgataaatag
    17941 atagcagtcg gtccatcggc tcccccgatt atgccaatcg gagcagcttg ttggcctgta
    18001 aagcccaaaa gaattgctcc taaaaatgtt atgaaaattc caaactgagc ggctgcccct
    18061 aataataagc ttttagggtt ggctattaaa ggtccgaaat ctgtcatcgc accgattccc
    18121 ataaaaatca aaggggggaa aataccaagt gcatttccgg tatagaaata tctaaataag
    18181 ccggcgggtt tcccgtctgc agcatggatc attaaatttg tcatcggtaa attcactaaa
    18241 agcataccaa aagctattgg taccaataac aaaggttcat acttttttac gataccaaga
    18301 tataaaagga ctaaagatac tattatcata atgatctgag ggatatccag agccattata
    18361 ccggtttttt ggaaaaattc tataaaaacc tgtaccaatt ttcttacctc ctaacatatt
    18421 gtgacttatt ctataactac taatacttct cccgcttcta ctttttggtt ctcatctaca
    18481 tttacctcac taacctggcc agaagtaggg gcatatactt cgttttccat cttcatcgct
    18541 tccagtacta aaagtaagtc tccttttgtt acttcttcac cttgcttaac ctttacctct
    18601 aagactgtcc ctgtcatagg ggcttctact gtgccggctc ccctactgga actagcagat
    18661 tttcttggct ttttcttggc ttttgaactg gtctctgcgg tagatgttga agttttaggt
    18721 tgtggtgcat gttctctgtt ttgagtttcg gattgaacta ctgactgttg gtcagtttcg
    18781 acttcttcta cttcaacttc atataattca tcattaactt ttactttaaa ttttttagtc
    18841 ataataactc tcctttatta tttgctgata ttccgccgcg ggtgactcta ccccagatcg
    18901 gcacatcgtc tttaactctt gtaatctttt ttatcttaaa ttttttctgc ttacttgaac
    18961 tttctatact catgatggcc gccataattg ctgccacttc ttttttatgg tctatatcag
    19021 gttctaaatc tgccacttta ggctcaactt tttttgcatc ttctttaaag tctatctttt
    19081 cagaataaaa gatcttcttt aaaatattta ccactatcat caaaagaaag agaatcaaaa
    19141 atactattaa catacctaaa atagctacct gtagacccga aattaatttt tgatttaaag
    19201 tcattgactg tactaaattg gggtctctta ataactcgag aggattcaca ttaatgcctc
    19261 ctttttatcc tataaaggaa tattaccgtg ttttttattg ggctgatttt cccttttacc
    19321 tattacagat tctaaggcca tgataatcct tggtcttgta ttacgggggt ctattacatc
    19381 atctatatag ccttcggctg ctgcccggta aggatttgca aagctttctc tgtactcttc
    19441 gattttctcc tgcctttttt cttccggatt ttcggccttt tttatctctt ttctaaaaat
    19501 tatattggca gcaccctgag gccccataac agcaatttct gcatttggcc atgcaaatac
    19561 atagtcagca ccaaggtgtc tactactcat cgctatataa gctccaccat aggcttttct
    19621 taaaataaca gttatcatgg gtacggtcgc ttctgaataa gcatataata attttgcgcc
    19681 gtgtctaata actccaccgt actcctgttc tactcccggt agatagccag gcacatctgt
    19741 gaaggtaaca atcggaatat taaatgaatc acagaacctg ataaaacgag cagctttatc
    19801 ggaagaatct atatctagag tacctgcttt aactcggggt tgatttgcaa tcactcccac
    19861 tgaacgtcca tttaaacggg caaaacccac tacgatattt ttggcataat acttatgaac
    19921 ttcaaagaaa tcttggttat caacgacttt ttttattaca tctctaatat cataggcttt
    19981 attaggatta atcggcacaa tatcttttaa cttatcttcc acacgttccg gggaatcgtt
    20041 agtttcaact acaggggctc cttctaaatt gttattagga ataaatgaaa ttaacctttt
    20101 tatctgttca aaactatgct cttcattatc agctaaaaag tgtgctactc cacttacttc
    20161 gttatgagtg gaacctcccc ctagttcttc agcactaact tcttctcccg ttactgattt
    20221 tactacctgt gggcctgtaa taaacatttt agaggtgtgg tcaaccataa agacaaagtc
    20281 tgtaatggcg ggagagtaga ctgctccacc tgcacatggt cctaaaatta cagatatctg
    20341 tgggattaca ccagaaatat cagtattcct tttaaagatt tctccgtaac ctttcagggc
    20401 gttaacacct tcttggattc tcgctccacc cgaatctaaa agtgcaataa atggtgaacc
    20461 agatttttgt gctaaatcca taattctaca tatctttttg gcgtgcatct ctcctaaaga
    20521 accgcccatt gtggtaaaat cctgagcaaa taaatataca gatcttccat tgatcgttcc
    20581 ttggccggtt atcacaccat cagccggaat ctctctttcg tccattccta aatcttgtga
    20641 atgatgctct acaaacatgc ctatctcttg aaagctacct tcatccaaca atctgtcgat
    20701 tctttcgcgg gcggttaact tacctttgct atgttgtttt tcaatccttt tttctccacc
    20761 gcctttagaa acttctcctc tttttttgtg tagttcttta attttatctt tcaactgggt
    20821 ccctcccttg atatatctat agttatattg tatgttacca atataatatt ttcaataatt
    20881 aaaaacagta tacctttgtt tattttaaca ataatttcat ttttttattg aaaccttaca
    20941 taattaagcc aaaatataat cctgatagca tatctagacc tgaactatta cccatttcta
    21001 ttaacttttc agtatatttt ctataatatt tttccccatt ttctatattt ttgtatatat
    21061 caactaatct tttattatat ctatgattaa ttgctccttt taaatgaagt gcactaacgc
    21121 gattagtttt atcaaatagc tcatctgttt ccatttcatt aatcacattc aatttatctt
    21181 ctgctaattt tgcttctatg actgataaat atcccactat aaagtcatct cctgttggtg
    21241 taaggccaat tcccaacccc aacaaattat tgatagaaat ctttttttct aagatatctt
    21301 ttaacttatt ataaaattct cgctgtaaag ttgataactt aaattcttgt atgttattta
    21361 ttatattagt aaactttaag attgtatggt ctaaactgcc tgaacggttg gtatttttaa
    21421 taaccttctc agtccattct attaaaagat ccctatctac aaatgaacaa tcctttattt
    21481 tgccactaaa aatgttttta ttattataat taaatatatt tttattataa attatttgtt
    21541 cgttgtttat ttttattttt gtttcttctt ctaagctatt tttgatattg ttgaatatat
    21601 aatttggtaa cttcacgtgg taaggagcta gggttacctt actgtcagtt attgcatagc
    21661 ctttattatt ttttgttttt atatttataa cattattaaa tttgctaatt atattaccat
    21721 ttaagctata attttctttt actaaatcac cttcaataat ataatcaaac attttcatta
    21781 cctaatatat cagtaaagtt tagcattttc aaagcaattt caagatttaa tttatcgtca
    21841 ggattatcaa ggcttaaacc tgtaattttt tctattctct ttaatctata atgaactgta
    21901 ttataatgaa catatatttt tttggctaca ttggtaagat ttccattttc ttcgaaaaaa
    21961 gcctttaatg tttttaaaag ttctgtatta tgggctttat catattttag taaaggaatt
    22021 aaggaatttt ttaaaaacct attcttttct tccctatcaa ctttatacag taatttatag
    22081 actcctaaat catcaaagaa atatacctga tcttttttat ttaatttatt ggctacattt
    22141 atggtccttt tagcttggga ataactttca tgtaaattaa ttatttcttt tttataattc
    22201 cctactccta cagttactaa gtctctatcc ttttgatcaa atacattcaa tactttttga
    22261 gaaaattctt tgatactctt tttctctttg ccaggatata aagctactag ataagtacct
    22321 aaatctccaa taattacctc ttcatcaatt attctattag ctgattctaa ataacttttt
    22381 tggatatttt cgtttaaaat ccccttcttt ttaaaatgct ttgaaattaa ctcttttaat
    22441 tcaaatacca ccactgaaaa acctttatct aactccatat ccatattttg accgcgattt
    22501 aatatagtct ttttgttatt aattttccct tttaaaatat cataaagtaa ctcgttttta
    22561 tattttaact ccacatttgc tatagagcgt ttatttaaaa tatcaagagc agctattgta
    22621 ctagcccact ttaatgttga tatatctaat tcatttatac tcttgccata ttcccaggca
    22681 aatagatagc caaaatgact attaggagtg ttaataggaa tcttcattag attaatttcc
    22741 ttaccatgca aagtaacatc aattttctta cagtggtaat taaagacctc ttctttagat
    22801 tctttttgat cattaataga tatgctgtca gtgagatctt ttttagtaac ttcattgtta
    22861 aaatcatctg gaatagaata cgagataata gctccattgg aatcggtaat taaaacagga
    22921 ttttttatca tatcaactaa aacagtggat aatctatcaa gtccattttc ataaagtact
    22981 gtattggtaa gtacttcatg gatatttaaa gttttttcta aaaatctggt ctgaacatta
    23041 agtatctcac ttaaaatagg gttgataaat actgaatagg aataatcata aggaatttta
    23101 agtaaaggaa agttatgtag ctcagcttgt tcaatcataa atgaaggtat ctcattaata
    23161 tacctacctg gttttatacc taatccagct aagttttttt gagctaaatt aggtattaga
    23221 ttttttaagg ctttttcatc gtctttaatt gaatacagtg tggtgaataa aagttctcct
    23281 tctttaaccc agtctaaaat atcaggaact tccataatgt tgacctttgt aatatgacgt
    23341 tctaaaccat ctttacccgc taaaatttca acttctttca tcttttctag ctttaaagca
    23401 tctgccatag ttattccaat taactctttc atcaatttca ccatcctgag aaaagtttat
    23461 ttgtaaataa tacatctaga attataacac agataaactc aatatggaag aactcacaac
    23521 aatttttcct taagcatttt attgtttgta aatattcaat aaaaaaaggc aacccataag
    23581 gttgccttta ttgataatta ttataagttt attattaact taattattat atctctatga
    23641 tatttaactt gtttttacat ttgggcaaat tcttcttctc catcgggaag aattagattc
    23701 aagacaacac cgacaagtgc tgccagagct aaactagaaa attctattct tccgagctgg
    23761 agagttgctc caccaagacc aagtacaata actgttgcaa cgataactag gtttcttgaa
    23821 atcttaagat cagtttgact ttctactaaa gtcctaatac caatagtagc aatcataccg
    23881 tataagagga atgaaatacc accaataact ggattaggga tggtttcaat tagtccaccg
    23941 aacttcggaa caatagccat tgcaattgca aaggtagcac caattctcat aacaactgga
    24001 tcatatactt tagtaatagc taagacaccg gtgttttctc catatgtagt gtttggaggt
    24061 ccaccaaaga tacccgcaag tgaagttgcc ataccatcac caaacatagt tctatgaata
    24121 ccaggatctg taactaattc tcttccctta ccaattgttt cactaatagc taaaatatct
    24181 ccaacgtgct caaccattga tactagtgca acgggagcta taatggtaat agctgtaata
    24241 ttaactgctg gaactgtaaa ttcaggaaga ccaatccagg cattattaat tactggtgaa
    24301 aagtcaacca tgcctacaat taccgccacg atataaccag ttaccaagcc aacaatgata
    24361 ggaacaagtt taaaaaatcc tttagcaaaa atagcagcaa taaccgctcc agctaaagct
    24421 gcaatagcca caacaacatt aagtgcgctt cccgttgaca tatcaattgc agttggagct
    24481 aagattgtac cgataagaat aataactgga ccagtaacaa ctggtgggaa tattttctta
    24541 attaaactag gtcctacttt ataaatgata aaggcaacaa tagcataaat aagaccagct
    24601 gctataatac caccctgtgc agctggaatg ccaccatgct gttcaataac taatgatata
    24661 ggtgcaataa atgcaaatga agaacctaga taggcaggta cctttttctt tgtaatgaag
    24721 tgaaatagcc aggtaccaac acctgcagta aatagtgcaa cacttacatg tagccctgtt
    24781 aaaattggta ctaaaatggt agctccgaac atcgctaatg cgtgctgtag gccaagaaag
    24841 atcgcttgcg atttgggaag gttactaaca tccctcacag cttgttcttc tcgtgattcc
    24901 attaattatt cctcctttaa aaataagata tgatattaat ctgtatttaa tatgatatta
    24961 atttatactc tctactcacc tcctattaat ggtttaaaaa gattattatg gtaattattt
    25021 caaatactac ccctatatat taattctcac tatattcctt attctccttt tgtaatatta
    25081 aacaatttta cctaacattt ttcgttatat tttctaatgt atttattata ataatctaat
    25141 ataatgataa tgatttaaaa acacgggaaa ttatagagga ggatattata ttggaaggta
    25201 tcaaattatt aaaatctaga agatccattc gagaattcaa agaacaaaaa gtagaaaaag
    25261 aaaaactgga aaaaatagta gaaactgctc gttatgcccc gactgccaat aatgtgcagc
    25321 cctggcagtt cataattgtg acagatgaaa ataagataga aactttagaa aaatatattg
    25381 gtcaaggcaa agaatttcca gcctgtatag cagtttttac aaaagatgta aaacatcatc
    25441 ttgaagatgg ttctaatgca actacttata ttttattagc agcagaagaa ttaaaattgg
    25501 gcacatgctg gattggaagt tacggaaaac cttattcctc tgaaattaaa gaacttttaa
    25561 atgtaccaga aaattacaag ttaatttcta tggtaacagt cggttatcca gatgaaaatc
    25621 cacatcctga taaaaaagaa ttggatgatg taattaattg ggaaaaattt taaagtataa
    25681 aatcaagtta gataattaag ggtacggatt tgagccgtac ccttttttat tttaataatt
    25741 ttcttctatt ttattaatta ctagacaagc attttgtcca ccaaatccaa atgaatttga
    25801 gagcactgct tttaggtcac taacttctct tgccttatta ggtacataat ccaaatcaca
    25861 ttctggatct ttattttcat agtttacagt aggaggtagt atgtcatttt tgatagctaa
    25921 tgctgaaaca atagcttcta ttcccccagc tgcccctaat aaatgtcctg tcatagattt
    25981 tgttgagcta acagccaagt tttcaatatg ttctttaaac agatttttaa tagcttttgt
    26041 ctctagttta tcattaaaag gagtagaagt accatgggca tttatgtagc ctatatcttc
    26101 atatgctaca ttagccttat taacggccat ttccatcgcc ctataggctc cttctccccc
    26161 gggagcaggt tgggtcatat gataggcatc agcagacatt ccatagccgc ttatttcagc
    26221 tatgatattg gctccccgtt ttttagcaaa ttcataatct tccaggacta atattccact
    26281 tccttcacct attacaaaac catctctttc tttatcaaaa ggacgactgg ctttggttgg
    26341 ttcatcattg cgggtggaaa gagccttcat attactgaat ccagctactg ctgaaggtgt
    26401 aatagaagct tcagctccac cggaaatcat taccttggca tctcctcttt taataatctc
    26461 catagcctca cctatcgaat gggtaccaga cgcacaggca gttacctcat tactgttcgg
    26521 acctttagca tttgcataaa ttgccacttg tccggctgcc atgtttgaga tcatcatggg
    26581 aataaagaaa ggactgattc tacggggccc tctttttact aatctttcca tctgtttttc
    26641 aaatacttca ataccgccaa tacctgaacc aattatgaca ccggtatcat tggcaatatc
    26701 atcagtaact tccaacccgg cattttccac tgctttcatg gctgcataaa ctgcatattg
    26761 agtaaatttt gccattcttt tggcgtcttt ccggtcaata taatcaccgg gatcaaaatc
    26821 ctttatttcc gatgcaatct gactagaata tccttccggg tcaaaacctt caattttaga
    26881 aataccagac ttaccattta taatattatt ccagaactca tcgatctcca taccaagtga
    26941 agatacaaca cccagtcctg taattacaac tctattactc ataacaaagt tacctcctga
    27001 tttatatttt tttcattgct ttttctaaac tagcactatc ctgtatattg tatgttttga
    27061 tagatcgatc tattcttcgc attaatgact ttagtactct ccctggtcca acttctaaag
    27121 cacattctat gccattttca cccataagtt ccatactttc tacccatcta actggattta
    27181 ttaactgttt ttctaaagca gttactattt cctctttgtt tttaacaaaa tcagctgtac
    27241 tatttgctat aacaggaact tttggatctt taaactgaac actttgaata tattctttca
    27301 gtttattttt agcaggttcc attagctgag aatgaaaggc cgaacttaca tttaattcaa
    27361 caatcttttt tgcgcctgct tcatcagctt tttccattgc ggtatggact gcttcttctt
    27421 cacctgatat tacaatctgc atcggggaat taatatttgc tagttcacaa actcccgcaa
    27481 ctttcccaca tatctcttct attttttcac ggtctaaagc tattatagct gccatggttc
    27541 ctttagaacc atcagccaca gccgaagcca ttaattcacc tctttttcta actaacttca
    27601 aaccggtctc aaaatcaaat actcctgcag cacaaagcgc ggtatattct cccaaactat
    27661 gtcctgctac catggaaggt tcaacacctt tatcttttaa tatttcataa gctatcatac
    27721 taaccgtaaa gatagcaggt tgggtgtttt gagtttcggt taactcatct tccggacctt
    27781 caaaacatag tttctttaaa tctatatcta atacctggtt tgctttatcg aagtattctc
    27841 tggccacagc aaagttatca tagaaatctt tgcccatttc tacatactga gaaccctgac
    27901 caggaaataa aaaagctata ttctcactca ttgtagctca ctctcctatt taaatatttt
    27961 gattccactc taacacgcca gctgcccagg tgagtcctgc cccaaaggca actaataaaa
    28021 cgtggtctcc gttttggatt ttaccttttt caactgcttc agtcagagct atagcaacgg
    28081 atgcactgga agtattacct accttcggta aattaacgta tacttcttcc tcatctaatt
    28141 ttagcctgcg acgggccgat tttattatac gggtgttagc ctgatgtgga atcaataaat
    28201 ctatatcatc agttgtaaga tttgcttttt ttaggacgtc tttagaagca tttttcatag
    28261 ttttaacagc aaacttaaat accggattac cttccatacg tatatagtgc tgcctgtttt
    28321 ttaccgtttc taggctggca ggtttcttgg agccacctgc ttccatatag agggcatcgg
    28381 caccactacc gtctgaaccc agatcaaagg aaagaaaacc acctttttct gtgggttgta
    28441 aaacagctgc tccagctcca tcaccaaata ggacacaggt atttctatca tcccagtccg
    28501 taattttgga aagagtttca gccccaatta ctaatacatt atcatatagt ccactttcaa
    28561 taaattgggc tcctacactt aaaccataga caaaacctga acaacctgct tctaaatcaa
    28621 aggcagctgc attttctgcc cctattgtat cctgaattat acaggcagta gctggaaaag
    28681 ccatatctgg tgtaaccgtg gaaacaataa tgaggtccaa ttttttggga tctaaattgg
    28741 ctttatttaa agcttcatag gttgctttta aggctaaatc agaagtggaa gtactttcat
    28801 ccgcaattct tctttcttta atacctgttc tttttcgtat ccactcatca gtagtatcaa
    28861 ctattttttc taagtcttta ttattcataa cattcggagg caagtattta ccccatcctg
    28921 ttatagttgc tcttctcatt tatatatatc cacctttctt acttgctttg ataattgttg
    28981 aagaaaaaac ctgatcgacc acctgttttg cccttatcaa cctcctttaa tttaaccaca
    29041 aaattttata tcagaaaaga gaatattatt ctcttatata atctttgttt ttattaataa
    29101 cttcaattgt ttcatttata atatcatcaa taatttcttg gaccggtttg ctatcttcta
    29161 tcatgccggc aatctgaccg gccattacac taccagtatc tatattacct tcccgaactg
    29221 cttctcttag tttaccagaa cccatttctt caattgtctt tttatcaata ccttcttttt
    29281 ccatattatc aatttttcgg gttaatttat tttttaagtt tcggaccggg tgaccggtac
    29341 tacgaccggt taccaccgca tctctatctc gagcagaaag aatagccttc ttatattttt
    29401 tatgagcagt acattcttct gcacatacaa atctggttcc catctgtact ccggacgccc
    29461 ctaaggataa aacagcagct aaaccccttc catctgcaat accacctgct gcaatcacag
    29521 gtacatcaat tgcatctact agttggggta tcatcgtcat agtagttaaa tcaccaatat
    29581 ggccaccggc ttctgttcct tctgctatta cagcatctat atctgatctt acgagtcttt
    29641 ttgccagggc aactgaagaa acgaccggaa ctacttttat attattagct ttaaatcttt
    29701 ctatatattt accgggatta ccagctcctg tagtaactac gggaacctct tcctcaatta
    29761 ctaaatctac tatatcatca acaaaaggag ataaaagcat gatattaact gcaaagttat
    29821 tatctgtaat atcttttact tttttaattt cttctttaat tacttctttg ggagcatttc
    29881 cggctccaat aatacctaag ccgccggcct tagaaactgc agcagccaac tctcctgtgg
    29941 ctacccaggc catacctccc tgtaaaattg gatgctttat atcaaataaa tcacatattc
    30001 tggtatcaag atacatctta attcactccc gtttaattta ttcaatttca agtttacttt
    30061 caattatttc tactacattg ttttttaccg cttctctagc tacttttata gccccatata
    30121 ttgcctttgc atcggaacta ccatggctta ttattacaag cccgttaaga cccaataaag
    30181 gggctcctcc gtactgtcta tagtcaactt tatccttcat cttttttaat tcatctttta
    30241 gaagaagggc tccaattttg gcctttaagt tactggttag ggtatctttt aataaatcaa
    30301 atatatagga agctacacct tccgtggttt tcaaaacaat attgcccaca aaaccatcac
    30361 agacaattac atcacagcta ccgttaaata tatctctacc ctctacatta cctataaagt
    30421 tgtctattct ttcttcttcc tcaaataatt cataggattc agcatttaac ttgtttcctt
    30481 tttccttttc ttctccgatg cttaaaagtc ccactctcgg attatttata ttcaagacct
    30541 gttctgcata tatttgaccc attattgcaa attgtatcag atgttgaggt ttagagtcaa
    30601 catttgcccc tgcatctaag acaaccgtct tccctatttt agaaggaaat acagttgcta
    30661 ttggaggtct ttttacttct gaaacccgtc ctattttaaa gataccggct gccatgactg
    30721 cccccgtatt accggctgag atcaaggcat ccgccttatt ttcttttact aagttagtcc
    30781 ctactactaa agaagaatct ttttttctgc ggagaacctt tgatggttta tctgccattg
    30841 atacagtttg ttttgcatca acaacttcaa cagattcatt attataattt aattgttcta
    30901 atatactttt tatcttgttt tctttacctg ttaaaataca tttaatatct ttaaattcat
    30961 ctacagcttt gacaactccc ttaataattt cttcgggagc tttgtcaccg cccattgcat
    31021 ctactgctat tttcaaaacc atcatctcct tctttctgga ctataattat atattatata
    31081 tttaaattaa tattcctact taattactaa atttttttat gtataataaa aaaaggcagg
    31141 ccctttttag ctagacctac cttgatttga attaagtgtt aattactttt tcacctttat
    31201 aatatccgca gcttggacat gctcggtgtg ataatattaa ttcattacat tccgggcact
    31261 ctaccaaatt tggagacttt aatttccaat gagtgcgacg ttttctcttt ctggatttag
    31321 aagttcgttt ctttggaacc gccatatcct aacacctccc attctttctt ctatttttgt
    31381 ttgaaatctt ttagtttagc cagtctcgga tcaactatct cttcttcaca atcgcattca
    31441 ccttcattta agtcctgccc acatactgag caaaggcctt tacaatcttc atcatgaagg
    31501 ggcttgattg gaatttccaa gataatatcg cttaccaaga tcggaaaaac attaacattg
    31561 tgtaaatcct caatatcttc aataaataac tcatcgctga tttcaacttc gattggataa
    31621 ggaaattctt tgagacatcg gctgcactct aagatcaagg taccttcaat ttgtccggtt
    31681 aatacaaatg aatcttctgc ttttaataca tctaaattaa cttttaaggg accttcaatt
    31741 tcaatttcct tatgccgaaa gtctatatga tcaatatcaa gttcttttac gtactcccgc
    31801 ttactcccta cttccattaa atcactaaga tctaaaatca ctttacttca cctctgtata
    31861 ttaaccagag ttaattatat aaaatttcat aaatcattgt caagtaattt attgattttc
    31921 tattatttct ttggtatctt gcgcaataac taactcttca ttagtaggaa ttacaaatac
    31981 cttaatatct gattcagatg ttgtaatttc tttctcttct cctctaactt tattcgcctg
    32041 agaatctaag tcaataccta gattgtccat tttttctaga atgtgttctc ttaccccacc
    32101 agcattttca ccaataccac cggtaaatac aattgcatca acaccattta aagcagccat
    32161 gtaggctcca atatatttgc gtactctata attaaatact ttatcagcaa gggcagctct
    32221 atggttgcca ttatgagcag cttgtcttac atcacgtgag tcattactta caccggaaac
    32281 tcctaataaa ccgctttttt tatttaaaat attatctata tcttgtatat ctaggtcctc
    32341 tttttccatt aaaaatggaa ttatagcagg atcaatatct ccacttctgg tacccattac
    32401 taatccttct aacggagtca tacccatact ggtatctaca gaatctcctc catcaacggc
    32461 tgctatggat gctccattac ccaagtgaca ggtgataatt tttgcatctt cagcatcttt
    32521 atccattaat tctgcacatc tattggaaac atatttatga gatgtaccat ggaaaccata
    32581 acgtcttact ccatattctt cataatattc ataaggaatt gcataaatat atgcttcttc
    32641 cggcatagtt tggtggaaag cagtatcaaa aacaccgact tgaggtttac ctggtaataa
    32701 ctctttgcaa accttaatcc ctgatagatt gtgaggatta tgtaagggtg ccaaaggagt
    32761 aacctcttcg atcttttcaa ttacttcatc tgtgatcaat gtggaatggg caaatttttc
    32821 tcccccatgt accactctat gaccgacagc attaatttca tcaatatcct ctaaaacacc
    32881 tacttcagga tctaaaagga tttctattac atgttcaatt ccttctttgt ggttggcaat
    32941 atcgatatct ttttctactt cctgaccatt atcattttca tattcaatag ctgaatcttc
    33001 gattccgatt ctttctacaa gtccttttgc taatactctc tcttcttcca tattaaataa
    33061 ttgaaattta atagatgaac taccactgtt taatactaaa accttcattg tttattcccc
    33121 ctgttgattt aatttttttt aaagattttg agcttgtgct gccgtaattg ctactacatt
    33181 gacaatgtct tccactgaac aacctctgga aagatcattg acaggtttag ccataccctg
    33241 taagataggg ccaattgcat ttgctttggc taatctctgt actaatttat atccaatatt
    33301 tccagcctgt aggtctggaa atactaaaac atttgctcgt ccggccacat cactatcagg
    33361 tgacttttgc tcccctactt ccggcactaa tgcggcgtca gcctgcattt ctccatcaat
    33421 tggaagatcg ggctccatct tttgggctag ctcagtagcc tcaatgacct tatcagctag
    33481 ttcatgttta gcactaccct ttgttgaaaa ggaaagcatt gcaactactg gatctacacc
    33541 gggtaataga ccagctgtgt cggcagaaga aactgcaatt tcagctaact gtttggcatc
    33601 cgggttggga ttaacggcta catcagaaaa aataaatatt ccttcatggc catattcagt
    33661 atcaggcaca tccataatca tagcgcctga gacaaccgaa atattttctt ttgttttaac
    33721 aatttgtaaa gcgggcctta aaacatctcc agtagcatta atagaaccag ctaccatccc
    33781 atccgctttt tcagtatgta ctaacattac gccgaagtat aagggatcga ctattttatc
    33841 taaagcatcg tccctgctta tccctttatg tttacgcaac tcataaaatt cttctgcaaa
    33901 atcttctttt agctcggatt ttgccgggtt aataatttgg acatcgggaa gtttaacttg
    33961 ttcctgctcg gcaatttctc ttattttagc ttcttcacct aataaaatta tatcagcaag
    34021 attttcttct tttattttag gaagagcttt aatcattcta ggttcataac cttccggtaa
    34081 aactatcttt tttttacttt cgcgtgcttt gtttttaaat tctttcatta catccatttg
    34141 taccactcct cagttagtat tatattacaa taatatgtaa aattctatat ataaatttaa
    34201 tataaattag actttgttat tatagatatt ttctgataat atattattgg aactattaat
    34261 tttaacaaaa ggtggtcaat atgaaagtaa caggcattat tgtagaatat aacccctttc
    34321 acaagggaca tcaatatcat atcaacaaag caagggcaat tacaaaatct gattatatta
    34381 ttgcagtaat gtccggccat tttacacaac ggggtacccc agctgtctgt gataaatgga
    34441 atcggaccaa aatggcctta gaaagcggag tagatctatt aatagagctg cctttgtgct
    34501 ttagcatccg cagtgcggaa tactttgccc aatcagccat gcagctgctg gctaaaacca
    34561 atttagttga tgatgtagta tttggtagtg agcatggtga tatcgatatc ttaaaaaaca
    34621 ttgcatccct tttgttagaa aataatcagt attatgagaa aaggttgaaa aaatatttaa
    34681 aagaagggtc cggtttcccg gccgcgcgca aaaatgcctt actcgattta gtaaaattaa
    34741 agagcaatac taactactgg tcacaacata atatatttgc aatcttaaat ggttccaata
    34801 atatattagg aattgaatac ctaaaagcca aacaaagatt taatttaaat ctggaagtag
    34861 atacaataaa aaggatcggg cctaattatt attctgaaga aaaaacagat cactatgtaa
    34921 gtgctacagc tataagaaat tctttaaaca ataatgaatt agatagtgta aaagaaaaaa
    34981 tacctgctat tacttataaa attttaaaaa aagaaatagc cgataataag atacctatta
    35041 aaaaagaaaa tttgggttta atcctacttg ctaaaattag aaaattaagt gttgagcagc
    35101 ttgtaaaaat acttgatata aatcaagatt tagcagttcg catcaaaaat gccgctaata
    35161 atagcggtaa ttataaacag ttgatggctt ccataaatac ccgctcatat acaaaaacta
    35221 gatttcaaag gattttatta aatatattat tcaacattaa taaagatatc ataaataatc
    35281 atgattataa agggccatct tatattagaa tcctgggctt taatcaaaaa ggtaaagatc
    35341 tattatcaaa aatgagtgaa aaatcttccc tacccattat tataaatccc tctgaatatc
    35401 ttaataattt aacttttagt tctgaagatc ttttaaaaaa ttcactcagt ttggatatct
    35461 atgccactga tgtgtatacc cttttatacc agaacgccag ctatagaaag gctaatctgg
    35521 actttactaa aaaggtgatt aaatcttttt aggctgcctt atctaaattt tgagatttta
    35581 aaaattcaat tgcgtcatca aaggtttcta ctgggaccaa ttttaaattt gtctccacag
    35641 ttttagcctt ttcatagttt tcttggggca caataaatat ttctgcttct gcattctctg
    35701 ctgctataat tttttgaaca atgccgtcta tttggccaat ttcaccttct aaattaattg
    35761 tgccagtccc cgctattcgc tttccatctg ttaaatcttc ttccactaac tggttataga
    35821 tttctaaggc aaacataccg ccggcagaag ggcccactat attaccggta tcataactta
    35881 cctgaatggg aaattcataa tctaagttct tggtacgaat taagattccg atagaaggtt
    35941 tacccggact ttcggacagt tcaattgtct caaccgtaaa ggccaatctt tcattatctc
    36001 ttattacttc aatattaacc ttatcaccca cttctctgtt tctaataata tcaactgctt
    36061 ccgaggcgat ctccactttt tgaccatcta tattctttat tatatctcca ttttttaatt
    36121 cattataagc ttgactggct tctagaactt ctattatttc aacaccttca ccctgaactt
    36181 taacctcata gcctaatttt cttaaagcaa ccgcttctgc ggtcatcttg ctttcttcca
    36241 tcaaatcaga catcatctgt agatattttt ccatatctat accttccggc agctgttcag
    36301 atagtatttc aagttcaaaa ccgtccggat taaaagtatt aacatatata taatcccaga
    36361 tcgtagcctt ttgacttcct accgctgtta ataagaaact acctttaata ttatccttat
    36421 gaccattttc aactgtaatg ataggtccta gctcttctgc aatcccgggt gacatcacat
    36481 aatagggagt gggtatgaaa ttaataatta taaataatat tattaagata cctattcctt
    36541 ttgttaattt atcttttctc atattttatc cactccttct ttgaagtatg tattactcta
    36601 tttcatattt ttctagtagt gctttttcta ccggtttagg aacaacattg tccattttcc
    36661 cgccaaattg ggctacttct ttgacaatac tggaacttaa aaaagcatat ttagaatctg
    36721 tcattaaaaa tatagtttcg atttcagggt ccaattcttt attcatggag gccatctgga
    36781 actccccttc aaagtcagat acagccctta aaccccttat gattgcacag gcattttttg
    36841 attttacata ttttgtggta agtccagtaa aggaatcgat tttaacactg ttaatatctt
    36901 gtgtggtttc atttaaaagt ctaactcttt cttccataga aaaaatgggt tctttattgg
    36961 ggttttggaa taccgccaca atgacttcat caaaaatatt ggatgctctt tttattatat
    37021 ttaaatgccc ttttgtaacc ggatcgaaac taccaggata tacaactcta tctaacattt
    37081 tctattcccc ctcataaaaa tatactttaa ttactgtttc attgtaattt ctatccttta
    37141 aaattttaaa ttcttcaaaa tcctcaatct cttcatccct atgatgctcg actataatca
    37201 aggctcctgc ccttaacaat tcattattaa ttagggaacg gagtgtctta ttaaccatat
    37261 tatttttata gggtggatcc aaaaatacta tatcatgctt tttattaacg gtttgagtat
    37321 aggtgaaaac attttctgtc attacctcac agctctcttc aaacccgcat tttttaatat
    37381 tttctctaat aatatctgca tatcttcggt tcttttcgat aaaagtaaca tgctttgtgc
    37441 cgcgactggc cgcttctaat cccaagctgc caaatccagc aaataaatcc aaacctgcct
    37501 cgaaggggaa atatggtaag atcatactaa atacagcttc tttaacccta tctaaggtgg
    37561 gtctaacttc tttagtatct atgcttttta atctaattcc acctgcttta cccgctatta
    37621 ctctcatttg agaatcccta cctaaataat tatttctagt tcctttaact tttcagctaa
    37681 cttatcatat tttaaagccc aatctttttg attggttaat tcatttgctt cccggtttgc
    37741 tttggccaaa atcttggtat ctttcaagat atcagctacc tgtaaatcag taaccccatg
    37801 ttgttttgtg ccaaaaaatt cacctggtcc tcttatttca agatccgctt ctgatatttt
    37861 aaatccatca ttggtctctg acataacttc taaccttttt ttggcttcct ctgtcttggg
    37921 attggcaatc ataatacagt aggcctgact tgcactcctg cctacccttc cccgcagttg
    37981 gtggagctgt gccaatccga atctttctgc attttcaata accataacat tggcagtttt
    38041 aacatcaaca ccaacttcaa ttacagtagt ggaaattaag acatcaattt tttgatttac
    38101 aaaatcactc attattttat ttttctcatc attactgagc tgaccatgca tggctttaat
    38161 tatataatta gataaataag tttcttctaa cattttttta acttcttcaa ctgaatatgc
    38221 atctaaatct tctgaaggtt caattaaagg acagaccaca tagacttgtt ctccctcatc
    38281 tattttatct ttcacaaaat tatatatctt accacggctt ttttcggtcc gccaggtggt
    38341 gatgaccggt ttccggccgg gcggcatttc atcgatgata gaaaggtcca agtccccata
    38401 aagggttaag gctaaagacc tcggtatagg agtggcggtc ataactaaca agtcgggatt
    38461 gtctcctttt tgttttaact gatacctttg ttcaactccg aacctatgct gttcatcaat
    38521 tacaattaat cctatatttg aataatcaat atctttttga aagagggcat gggtcccaat
    38581 tattaaatct gactcactat tttttatctt atctgtgatt tcttctcttt tttcaccttt
    38641 tatactacca attaatagct ctatattata gtcaaaattt tttagtatct ttttaatttt
    38701 tatgtaatgc tgttcggcta aaatttccgt gggggccatt aagatcccct gataaccgct
    38761 ttctatggtc tttattagtg acatgacggc aactattgtt ttcccggaac ctacatctcc
    38821 ctgcagtaat ctctgcatgg gatgcttgtt ttccatatct gcttttatct gctgccagac
    38881 atttttctgg gcatcagtta aatcaaaatg cagctgattt aaaaagtcgt caatcagccc
    38941 ggctgtctga gaatgcttta tccccttttg ttttcgatag tctttctttc ttttaagcat
    39001 cgctatttga aataaaaaga gctcctgaaa ggctaatcta tttctggcct ggacaaaggt
    39061 ctttctatcc tggggaaagt gcatgttgaa aattgcattt ctaatttcaa tataattatg
    39121 cttatcaata atataatcag gcaagaaatc atcaatattt tttaaatatt tctgcaaagc
    39181 ctgatatatt atatacctta ttcttttttg agttattcct tctgttagag gataaatagg
    39241 tactaccctg ccggtatgaa tggtaggaga atcttcttcg atctcttcat aaactggatt
    39301 tactatttct tttttattat attgatggta actgttttca tttaattctc cataaagatt
    39361 aaacttcttt cccttttcaa atacatcttt taagtaggct tgattaaacc aggtacagat
    39421 gactgtatct gtcccatcgg aaaaggttac cttgaaaatg gatttatacc tgccgcgtct
    39481 tattaattcc ttatttaata cttcggcctg gatactaacc gcctgaccgg gtctaataaa
    39541 tctaatttcc ttaaaatctc ccctatcttc ataatcacgg gggaagtagt aaaatagatc
    39601 ttttaaagag tatatcccca gattgtttaa tctcttttca aacttgggac ctaccccttt
    39661 taaataccta atttcctctg cacttgtttt actcatctaa gtatttacca cccttgaaaa
    39721 gaattatacc ataaactgtc ggaccagtgt gactaccgat tacgggtcct acattatttt
    39781 cataataaat atgtctaata tttgcttcct ctatcttaga attaaatttt tctctaaaat
    39841 ttgctgcata ctcttcttcc ccataaagaa aaccaaacca atactgtcca ggggattcca
    39901 gatttttatt taagtaatca tctaaaaatt ccaataatct ttgatgagtg ttttttctac
    39961 ccctaacttt atcaattggt tcaacactgg ttgaattaaa atctaacttc aaaaccggtt
    40021 ttaagttgat taaattgcct ataaaagatt ctgctttatt aattctaccc cctttttgta
    40081 agtaggagag gtcatcaact gttaaatata tttccaaatc ttctctcgcc ttttttataa
    40141 tgtctactat attttctaat tccaatcctt tatcaactaa tttagcagcc aatattacaa
    40201 gaaagcccaa gcccattgag gctgatttag aatcaattaa tgttatatta tagtcggggt
    40261 ttttttgtat cgctaattgg gctgactgct ttgtaccact caaatgactg gagagatgaa
    40321 ttgataatat atgatcatac tgtctgttca tctccctgta tttttgttca aattcaccgg
    40381 ctgagggctg tgatgtagag ggcattacat cagcctgttt tatcctttta aagaactgtt
    40441 gagaggtaat ttctgtctta tctttatact gctcctcccc aattctaact gtcaatggta
    40501 caaattctat attatattct tctaaaatat tctccggcag atcacaagta ctatcagtta
    40561 agatacctat cttcataaat aatcccctca cttatagttt attccataga tataatatat
    40621 gggtatagag gttggccgcc tttgtaaatt tcaacttcag cctctttaga tttggcttgg
    40681 aacttctttt taatctcttc tgcatcctgt tggctggctc catcaccata atatatagta
    40741 attaagtctt catcttccca tacttcatct attagatcgc ttattacctc tatttcacta
    40801 ttaccaaccg ctctaatatc tccgtttaca atacctatta catcatcttc atttatttca
    40861 aaaccattta ctttggagtc tttaactgca ttagttattt cgattgtttt aacataacta
    40921 atttcttctt ccatggaact tcttaactca tccaattctt tattatcatt aaagactaat
    40981 aaggcagcaa ttgcttctgg aattgctttt gtttctacaa ctttaatttc tttatctgat
    41041 aaagaagaag cctgttgggc agctgatatt atattcttgt tattagggaa gataattacc
    41101 ttttcctgtt ccatctgttc aactgcttct acaaaatcat tgatactggg attcatagat
    41161 tgtccaccgt caataattaa gtcaactccc aattccataa ggatattttt aattcctttt
    41221 ccctttgcta ccgctataat acccacttta cggtccagct gttcgctttt tgcactttta
    41281 atcagtttct cttcataatc ttcagtactc tcgtctttaa gttgttcaaa atcttctttt
    41341 tgttcttctt catgtttttt tcgcatttca gcattttgca ttctcatatt atcaatctta
    41401 atatctctca gactacctgt ttttagcgca tattctaaaa ttatcccggg atgattggta
    41461 tgaatatgaa ccttaattat atcatcatct cctactacca ttaaggagtc tccaaactgt
    41521 tcaatatcat ttttaatctc atcgatattt acattctgtt ctttgacatt tattagggtc
    41581 tgagtacagt aaccgtactt taattcttca gcaacttctt ctttaatttc tcttttttct
    41641 ttaacttctt ctctaacctc tactttaccg gtcaatccgg ctttcatacc ttctaaaatg
    41701 gttaaataac cctgcccgcc tgcatctact acccctgctt cttttagttg tggtagttgg
    41761 tcaggtgttt tatttaaagc cttatgagca gtctcaatta ctttttctaa gaatacttca
    41821 atattttttt cgtcttctaa tttttctgct ctgcgggccg cttttcgggc aactgttaat
    41881 attgtacctt caacgggttt caaaacaccc tgataggcaa tcttagaagc cttagataaa
    41941 gaatttataa gatcgtcatt tctcattacc ctattgtttt tatttgcttc tgaaaatccc
    42001 cttagcagct gagataaaat cacgccggag ttacctctgg cccccattag cgctcctttg
    42061 gaaagggcct gtgttaacct atcaattctt tccgatttag ctttatcaag gttttcaacg
    42121 gcttccttga aagttaataa catatttgtt cccgtatctc catctggtac gggaaaaaca
    42181 tttaaagaat caatatagga ctgttgatct tcaagtctat ttagtgctcc atagcacata
    42241 tttttaaatg cttcaccgtt tattacttta atttcctcat tctgtgccat tacctattct
    42301 cactccttgg acgtttatct cgatactctc tacttctatt cctactctgt ttttgagcgt
    42361 atatttaact ctttcaataa tattatttgc gacttcatgg atgttggttc catattctac
    42421 tacgatattc aaaatgatat taactgcatc ttcttcaatt tctacagaaa cacctttact
    42481 taaattatcc cagccaagga gatctgctat tccatcctgc atgtttttag aagacatccc
    42541 cactagacca tagcattcca ttgcagacaa tcctgctata gtctctatga cttcttcagc
    42601 tattaatatc ttaccatatt cggtattaat ctcttttacc atgattctca cctctcttta
    42661 tatacattgt aattttacta tatattaata aatcttgcaa cattcttaaa atataacaat
    42721 aaatacttgt cacaaccata agttttgtgt taaaataata tttgactgaa aaagttatat
    42781 aagatacagg aggtgtaaca aatggctaaa tattgcgaga tatgtggtaa aggtagcaat
    42841 aatgcaaata gagtaagaag aagaggtaaa gctaagaaaa aaggtggtgt cggtcgtaac
    42901 gtaacaagtg taaccagccg cacccaaaaa cctaaccttc aaaaagttaa agccatcgta
    42961 gatggtaaga aaaaaagaat aacagtctgt actagttgtt tgaaagcagg taaagttaaa
    43021 cgtgcatatt aatagatatt taatgatggg gtttattcat gagtaatgga taaactcctt
    43081 tttatttttt gcttaagtta tttttaacct tttctgtaat tatctctaag gttaaatcct
    43141 gatgggctac accagtttta accgcttctt cagcaactgc caaagcagtt acataagcta
    43201 cctctttatt aaatacctct ggtataatat tttccttatt taaattatcc tttacaattt
    43261 cagcaatctt ttctgctgct tttaacttca tcttttcatt aatctcacta gcccttgcat
    43321 ctaaaacacc cctgaataca ccgggaaaag ccaaaacatt attgatctga ttcggataat
    43381 cggaacgacc tgtagctaca ataaaagctc ccgcctgata ggcttctttg gggttgattt
    43441 caggctccgg attagccagg gcaaatatta tcggcttctc attcattgtt ttcaccatat
    43501 aggattttaa tataccggct gtggaaactc caataaatac gtccgagcct tttatagcct
    43561 cttctaatct accttctttt ttactttcag tcatctcggc tagtttttca ttgtattcat
    43621 tattactttt tctaccctta tatactaccc ctttggagtc cagtacttta atatttttag
    43681 caccagcagt tttaagtaat ttagttactg caaaaccggc cgctcctgcc cctgtaatta
    43741 tgatcttgat ctcttctatt tttttatctg ctaatttgag ggcattaaat aaaccagcta
    43801 aaactacaat tgcagttcca tgctggtcat catgaaaaat cggaatatca gtttttgctt
    43861 taagtttctc ttctatttca aaacaatagg gggctttaat atcttctaaa ttaataccgc
    43921 caaaagaatg actgagatta actatatttt ggacaaattc atccacattt ctacgtgtta
    43981 tacataaagg ataggcatct acatcagcaa attctttaaa aagaacggct tttccttcca
    44041 taacaggtag ggctgctttc ggaccaatat ctcctaaccc caatacggca ctaccatcag
    44101 aaacaatcgc cacactatta tttttaatag tataatcata aatactttct tcatctttat
    44161 aaatttgttg acaggcttca gctacaccgg gcgtatataa taagcttaaa tcccttttgt
    44221 ttttgatctt ttgcttgctt ttaatactta atttcccttt taattttttg tgtagtttta
    44281 aggcttcttc ttttgtatcc atatgattac cccagttctt ctttatattt gttttgccct
    44341 atttcatata aatcttgtcc gtaaatatca ttgattacta ccacgggaaa attctttact
    44401 ttaagaagac ggatagcttc agtttgtaac tcgggatagg caacaatttt agattccatt
    44461 atacttttcg ataggagggc agctgcccca cctaccgctg caaaatagac tgctttattc
    44521 tttttaatgg ctttttttac tacctcagac cggggacctt ttcctatcat accacttaac
    44581 ccctgttcta atagagcggg cgtatattca tccattctat aactggtggt aggaccacag
    44641 gaacctataa cctgtccttc tcgggacgga gctggtcccg tataatatat tactgcatcc
    44701 ttaagatcaa tgggaagctt tttatttttt tttattaaat cattaagctt cttatgagcc
    44761 tggtcccttg cggtataaat ataaccactc agttctacct tatcaccagc ccttaatttt
    44821 ttaacctttt tttctgttaa aggagtttct agtttcatct tttcccccct aaatttcgat
    44881 tgaaaaatgc cgggaagcat ggcagttgat atttactgca acaggcaaag aagcgatgtg
    44941 acagggatag gttaaaattt taacatctaa ggccgttact tttccgccta atccagccgg
    45001 tccaatcccc gttttattaa cttcccttaa tatctcttct tcaaattgat taatcttctc
    45061 atctttactt tcttttttta acaaagcttt cttggataga taggcagctt tttcaaaagt
    45121 tcccccaata ccaaccccta gaatataggg aggacaggca ttagcgcccg catttttcac
    45181 gacatcaatt ataaattttt gaatatcttt tttatcagca gttggattaa acatccttat
    45241 tttactcatg ttttcactgc ccgcaccttt gattaataca tttaacttca gtttatctcc
    45301 cggtacatcc tcaatataaa tcacggctgg tgtattatca tcagtatttt ctcgtcgaaa
    45361 gggagtttca actacggatt ttctaagata tccttcatta tatcctattt tcaccccata
    45421 atttatggct tcttctaaac ttccttctat atatacttca ttacctattt caacaaagac
    45481 aactacaata ccggtatcct gacagatcgg tatcttttct ttttcagcaa ttttagcatt
    45541 ttctatgagt tctttcaaaa catttttacc caaacgtgat tcttcttttt tgaccgcctg
    45601 ttctaatcta ttatacatat ccttattaag tgagaaatta gcagtctgga ctgcatctgt
    45661 tatctctttt gttataattt cactatcaac tcttctcata aaatattctc ccttaacatt
    45721 tttcacagac gaagtataat aatttaccta aatctacttc aattactgct ttgtttgcat
    45781 ttattatatt acttaatccc cgactgctgg ctctttctaa ccagtaatta tctaattcat
    45841 acttacaacc ttttaatttt ttaattaaaa cttgatcagt aatagaaaaa agtgaaaggg
    45901 tatttccctt tttgttatca attatccttt tattatcgat taaaccaacc tctaaacccg
    45961 gttcaattat tttagcatca atatcactat ttaatgcata ttctaaaagt aataaattgc
    46021 caaactgctg gtcaattctc cccccggtgg cacctataaa actgattttg tttaaaccgt
    46081 tttttacaca gtaatcaata gataattccc catcagtttt atctttatca ctgggaaatt
    46141 catatatttc accgttgaaa tcttttaaca ttgcagaatc cctatcaata gaatcaaaat
    46201 ctccaatcaa ggtatcaggt tctgtatcta caaacttctt taaaaattta tagccgccat
    46261 caacggcaat aatattctca aaggaattta caatctcttg atatttttct ttttggtctt
    46321 tatatacacc atttaataca accaatactt ctttcacaat gatcactaac cttttagaat
    46381 tcttctaaat ttttttaaat tctgggctgg attaccttta aaaatggtag aaccacttac
    46441 tatgatatca actccggcct ccacaacctc ttttacatta tcaaagttaa taccaccatc
    46501 aatttcaatt aaagtttctg tattactttt atcaatcatt tttcctaacc tttttacttt
    46561 atcaagcatc tggggaataa atttttgtcc tccaaagccg ggattgacag acatgattaa
    46621 aaccaaatcc acatcatcaa taatattttc tatagttgat aaaggagtag ctgggtttaa
    46681 cgtaactcca gcctgacaac cttcattttt tatcatttga attgctctat gtacatgatc
    46741 agtcgcctct acatgtacag taatgatatc actacccgct tcagcaaaat ctttaatata
    46801 cttttcgggc tctacaatca taagatgggt atcaaaaata ttatcactat atggtctgat
    46861 tgctttaact actaagggtc cataggtaat attgggcaca aatctaccat ccataatatc
    46921 taaatgaagg tacttagcgt ctttcacact ttcaacttct ttttttaatt tactgaaatc
    46981 cgaagctaga atggaaggtg caaaattaac catataaatc atcctctttc tgctgaagtt
    47041 ctttatatat atctttataa ctattgtagc gctcctgtga aataaaacct gcttctaatg
    47101 cttcttttac tttacagcct ggttcatgag tatggctgca ggtactaaat ttgcagctgt
    47161 tttcatactc ttcaaactcg ggaaaataat attttaaatc tgaggaatca atattttcta
    47221 tctctatact gctgaaaccc ggggtatcag ccacaaaacc acggctgggt aaagtaatta
    47281 actctatatg ttttgtagta tgtttaccac gatttaattt tttacttact tctctggtcg
    47341 gaagatctat atcttcaaca atactattta tcaaagtaga cttaccactg ccactggggc
    47401 cagctataac cgaaatatta ttttccaact ttttatatag ttcatttaga ccgaggtgtt
    47461 ttttggcact aatatagaaa acgtcatagc caatattttt ataaatttga agttcttctt
    47521 tgacttcttc taaattatca actaaatcaa ctttattaaa aacaactaga ggttttaact
    47581 cagaagactc aaccattaaa ataaatctat ccagtaattt tttatcaaag ggcggtttct
    47641 ttaaagagtg taatacaata acctgttcta cattggcaac cttgggcctc attaagaggt
    47701 ttcccctttc tttaacagac tctactatct tgtcttctaa attaatttca gctacatcac
    47761 cgggatagac ttttttttgt attttgcctc taatccgaca tttatataca tcattatttg
    47821 tcttgacaaa aaagaaacca cccagtgttt tagttattat accttccatt aaaaacctcc
    47881 cagttaagca cctatttctt ttgattttat taactcccca tccctatata tttccaggac
    47941 ggttggtccc acactatata tcgtttcatt aacgtattcg ccgggactat gagttttttg
    48001 ataagcaact tcttcaccat tactatcttt tacaacaatt ttgatttcct gattattact
    48061 acccacaata ttgattccga tattggtttt atgttcttgg aaatcacctt tgttgactaa
    48121 accagtacta ataccaagat ctaccgttga attttccggt acattttcgc ctgcctcata
    48181 ctgctggttc ataattaaac cttcttcata tcgggtagat tctccataat ttaattttcc
    48241 cacttttaat ccatttcgtt ggattaagtt aaaggccctc tctttatcca cccctaaaag
    48301 ggacggcata gttaccatga tgggttgagg tcctttactc actacaagtt ccacctgcgt
    48361 atttatctca gtttcgctgc ctggttcagg actctgtgaa attatataat tctcgcggta
    48421 ctgttcacta tactcataac ttacttcttg taaagacagg ccgagattat ctaaaattac
    48481 ttcagcctct ctcagaggcc tattattgaa atcaggtagt tcaacaattt taggtccttt
    48541 acttaccgtg acaactacat ttctggtctg tttgatacgt tcacctggtt tcggttcctg
    48601 ggtaactata tatccttctt caatatcatt atgaaatact gcttcctccg atttttcaag
    48661 gttcaaaccc tgttgggcag caatatcctc tgcctcactg aattcttttt ctactaaatt
    48721 tggcacctta acaatcggaa cctcagtata tactttaaag aaatatacta gtccaaacac
    48781 ggaagcagat gccaaaataa ctactactaa cgcccagatc acccatttat ttaacttaaa
    48841 acctttcttt ttctcaggtg tgaagaaact tttatcggtt tttgatgttt tagagttaac
    48901 ttttttatcc tgttcttttt ctttttctaa accttctttt ttgatatctg atttatttaa
    48961 aattttggtt tgactacctt cttcgcctgt tttttcttcc ttgaactggt cactagcttc
    49021 aatatttttt atagccatga taatttcttc ccttaactgg gaagcacttt cctgcctatc
    49081 ttctggctct ttagaaagag ttttcatcac cagttcgtta acttcttctg gtacagaagg
    49141 aaaaacttct tttaattctt tgggctcttt ttgaatgtgc tttaatgcta ccgaaatagg
    49201 gctttcccct ttaaaaggca cccgcccgga gagcatctca tataatacga ccccaataga
    49261 ataaatatct gaatatgtat ttatttcttt cccctgagcc tgttcaggag aaaaataatg
    49321 ggcactgccc attatagtat ctgtaattgt cattgtagaa gaagatacag ctctggcaat
    49381 accaaagtct gtaaccttta cctggttatt atcagtaatt aatatattat gaggtttaat
    49441 atcacaatgt acaatattgt tttcatgggc cacctctacc ccggctgtaa cctggttagc
    49501 aatatctaaa gcttccacta cagatagttg gcctctcttt ttaataaggc tttttaaatc
    49561 tttgccttca atatattcca ttactagata aaggctctct tcactttcta caatatcata
    49621 tatactaacc acgttcgggt gggttattct tgccacggct ttggcttcat ggcgaaattt
    49681 gttaatgaag tcttcatccg aaacaaattc tggtcgaagc atttttatag ctactttacg
    49741 atcaagtagt atatcctgtg cttcatatac caaggccata ccaccgctgc ctctctgttt
    49801 tttaatctta tatctgtcat ttaataattt accaatcata ttgacacctc atagaattat
    49861 aactttccca ttataattgt aatattatca gtaccaccat ttgttaacgc tttcgaacct
    49921 aactcttcaa tcatctcttt tatagattga tcctttttga atatctcttg aatattttca
    49981 aatctaatca tatcacttaa accatctgtg cacaacataa tgatgtcttc ctttgatata
    50041 tctactttat cagtttgaat ttccaaatct ttatcaatac ctaaagcctg tgtaataatg
    50101 tttttcttgg ggtgatcaaa ggcttcatcc ggactaattg atttattttc aattaaatca
    50161 tttaccaggg aatggtcagt ggtaatttgg tttaaatcac cgttaaaaag gtaaatccta
    50221 ctatcgccaa catgaccata atatagttga ttatctttta atatccccaa ggtaagggtg
    50281 gttcccatat cctggtaatt ttcataatcc tgagaacaat ccctgatatt ttggttagcc
    50341 tctgtaatca cagactttat ctgggggatg atctcctggt caaattcaaa cgtataatct
    50401 cttatactct caattgcaat ttcactggca acttcaccgg cattatggcc tcccatacca
    50461 tcggcaacgg ccagcactgc agtcttttcc tccggctgaa gtattagata actatcttca
    50521 tttttttgtc tgttattccc gatattggta aagatatgaa aactcatgat taacctccat
    50581 tttgataact agcacgcagc tgtccacagg ctgcttttat attagtacct ttttcaattc
    50641 taatggtaac aggaatattg tttttttcca aattatcttt aaaatagttg atgatatctt
    50701 tattaggtcg ttcaatattt aattccggta ctgggttgac cggaattaaa ttgacatgaa
    50761 ctaacttatt ttttaacagt gaaactaact gttcggcatg aactttctta tcattaattc
    50821 ctttcatcaa tacatattca aaggttaccc ttcgattagt tttatcaata tagtattgga
    50881 cggactccat taatttgtct aaatcatatt ttttattaat gggcattaaa taatccctga
    50941 gctcatcatt gggggcgttt aaagaaatag ccagaccgat ctgctgattt tcatcagcta
    51001 atcttttgat cttatcaata ataccggaag tagaaattgt cattttccgc atgccgatat
    51061 ttaagccttt cttatcattt aaaattgaaa cagccttatt taaattatca taatttaaaa
    51121 agggttctcc catccccata aaaactatat ttgttaccgg ggtatcttta tattgctctt
    51181 cacttatatc aatctgtatt ttcaggatct gttcaataat ttctcccgcg tttagatctc
    51241 tctttaaacc accgatcccg gtcgcacaaa atttacagcc aattgaacaa ccaatctggc
    51301 tggagacaca gatcgaatat ctattttcat cataatatgg taaaaagaca ctttctacta
    51361 atttatcatc agctgttttc cagaggtact tttgggtacc gtcttcagct attaattttt
    51421 tgtccaaaga aaactttgaa attgtatatt tactatctaa tttttttatt aaatcaaccg
    51481 gtaaattaga catcttatca aagtctaata caccgttttg atatatccag ttaaaaacct
    51541 gttcggctct atatgaagct atatcacttt gttgaaattc tttttttaaa ccatttaaat
    51601 caaaacttaa gatatgcttt ttattcatcg ctacccattc caaataatac aactatcctc
    51661 agcagattta ccagggccac taaagtagcg gaaacataag tgaaagcagc tgctcgcaga
    51721 acctttttag caccctttaa ttctttctgg tttaagatgt tgtttttctt aagcatcgtt
    51781 aacgctctat tgctagcatt aaattcaaca ggcagggtaa ctaaatgaaa tacaaaggcc
    51841 cctaaaaaca taataaatcc gagagcgatc ataaactgtg attgcaggaa aaatccgata
    51901 atagccattg gtaatcccca attagaacct atactggctg caggtactat cttagccctg
    51961 atctgcaggg gggtataacc ctttgcatcc tgaatagcat ggcctgcttc atgggctgcc
    52021 actccaatgg cagcaatgga tttactatta taaatacttt gggataaatt taatacttta
    52081 tttcgaggat cataatggtc acttaattct ccctggattc tttttatact tacatccttg
    52141 ataccgtttt ttgctaaaag ctcgctgacg atatcagcag cggtccgatt tacatttgta
    52201 tttatttccg aatacttatt aaaagtactt ttgactttat attgagcata cattgcaatt
    52261 aataacacgg gtattaacat taaaattgtg gggtcataaa aaaacataat agatcactcc
    52321 tttttgcttt ctaaccttgt tccttcctct atctcatatc ctcttaaaaa atctggtgta
    52381 gacattcttt ttttacccgc taattgtaac ttttcaattt ttaaaagacc tttaccggtt
    52441 tgaatatata aaccttcctc cggctcagct tttattactg taccggtttt tttatctgta
    52501 ttatcatcta ccggttttac ttcaaatatc tttaataatt taccgtcaag ataggtaaaa
    52561 gagcccggcc agggattcat agctttgaca aagtaataca agtcctgact ggacttttgc
    52621 caatctataa gaccgtcttc tttacttaat tggccggtgt aactagcttt atcttctgtt
    52681 tgactaagtc ggggtactga accttctttt agcttaacaa ccgtttctct taacagttta
    52741 gcaccaactt cggccagttt atcatgcagg tcaccggcat tatattcttc ttttattgca
    52801 acctctcttt ggtatattat atcaccggta tcaagcccct catccataaa catagtggta
    52861 actccggtgg tatctttacc atccattatc gcctgttgta tagggctaga acccctatat
    52921 tcaggtaata aggaggcgtg caggttgata cagcctgccc ggggtagatc aagcagctct
    52981 tttgataaaa tctgcccata agcaactacc accattaagt ctatatctct tttatttaac
    53041 ttctttataa aatcatcatc gtttatattt gcgggctgta atacctctat atcatgtttt
    53101 aatgcctctt ttttgacagg agtataccta acctttttac ccctgcccct tcttttatcg
    53161 ggttgtgtta taactgcctc aatatttatt ttatcttctt ttactaattc ttttaggctt
    53221 ggtacggaaa agtccggagt acccgtaaat ataatcttca ttttttatta tcactccacc
    53281 atttccggtt cttcatcttt tacttcaagg gctttatcta caaaaaggat accatttaaa
    53341 tgatctatct catgtaagaa agcccttgct tttaatcctt ctaattcata tttaacttca
    53401 tcaccttgct cattaaggcc tttgattgta acttcttctg ccctttttac aagaccagtt
    53461 ttaccgggca cacttaaaca gccctcttca gcaaccgctt cccctttctt tttaaccgct
    53521 acgggattta cagttacctt aagatcttct tctatttcaa caatgataac tctctgtaaa
    53581 accccaatct gaggggccgc taaaccaata ccttcagctg cttccattga atctatcata
    53641 ttatctatta attctcttgt tttatcagta atctcttcca cttcttttgc tctactgcgt
    53701 aagactggat caccaatctc tcttatggga agtactgcca tctttaccac ctctttaaaa
    53761 tattataaca tcgatattgg atccacatca atattatact ttacattcat actaatgttt
    53821 tttaaaaatt catcttttag tttttttaat atacttgtcc tattatcaag gtcatcgaat
    53881 tttaataaca actgcaccct atacctaccc ctgatccttt caataggagc tggggaaggt
    53941 ccaattatct tctttatttt ataatctttt atatgattat acaacttttt tgcagctttt
    54001 tcagctttat ttttcttttt atgatttatt gtaatgttga ccagatttgt aaaaggcggg
    54061 tactgcagtg ctttacgcag tccaatttcc tttttgtaaa aatattgata atcatgttct
    54121 ttagccgcta aaatactata atgatcggga ttataggtct gaatcgctac aacccctttc
    54181 tttttgccac gacctgttct gccagctacc tgagtcaata attgaaaggt tctctcggaa
    54241 gatctaaagt caggtagatt catcatagtg tcagctgtga taacacctac caaggatata
    54301 ttaggataat catggccttt tgccaccatc tgagtaccaa ttaaaatatc aatttcatta
    54361 ttttctaact tatttaaaat ccttcggtga gatccttttc tagtggtagt atcgtggtcc
    54421 atcctggcca cttcagcctc gggcagcaat tttttaactt ccttttctat tctttcggtc
    54481 ccgataccaa aatccttgat atacttgctg ctgcagttcg gacaatattt tggaatatca
    54541 cgggttgccc cacaataatg acagattaat ttatctttcc cctggtggta ggtcatcgat
    54601 atatcacaat tctcacattt aatgacctca ccacaacttc gacataagac aaaactggaa
    54661 tagcccctgc gatttaaaaa taagatggct tgctcacctt tacttaaagt ctgttttaaa
    54721 ccatcctcca atttactgct aaaaatagat gggttcccct tttttaattc ttccgtcata
    54781 tctataactt ctaccggggg catttctttg ttgtcgattc taactggtag agaaagataa
    54841 gtataatctc cttccttagc tttataataa ctttccaaag aaggtgtcgc tgaacctaaa
    54901 accagatcaa tattatttat ttcagccctt ttttgggcaa cttcacgggc gtggtaataa
    54961 ggatggttgc cctgtttata ggaattttca tgttcttcat cgataataat taggccgaga
    55021 tctttaaccg gtgcaaagat ggccgaacgg gccccgataa caattttagc tttacctctt
    55081 tttatattcc gccattcatc atatctttct cctgatgata gggcagaatg taaaacggct
    55141 atttgttcac caaagcgact ataaaatcgt ttcaccatta aaggagttaa cgatatttcg
    55201 ggtactaata aaatagctcc ttggccggcc tgtaatacct ctttgattaa ctgcatgtag
    55261 acttctgttt taccagatcc ggtcacccca tgcagaaggt aggtattgct tttgggctca
    55321 aataggttgt tattgatagt ttgaagcact tcggcttgtg ctttagtggg ttgtaattct
    55381 gctttaagct cattgtcata atccttatag ggaatccttc tttctatgtt ctctgtatat
    55441 tctagataat cttttttaac cagataattt atcgcacccc gatatacatc taatttatct
    55501 aaaagtcttt ttacctcaat ttcttcgttt tcattctctt taaaataatt taaaacctta
    55561 aattgactgg gagcacgatt ttcaagctcc ttaagctttt tatctatttg ctttttttct
    55621 aattttaatc ttaccgcttt aaccgttttt ttacttatct tacccttaaa aacagctgta
    55681 ggaacagctg ctttaattac actgattaaa agtgcacagt aaaattcaca tatccattta
    55741 tatagtttta aatcactttc tttaaaaaat atttcatctg taataagact atctatctct
    55801 tttaatttct ctttttcaac ctctgtttca tctttgatat ttaaaataaa agcccttatt
    55861 tttcgctttc caaatctaac cttaacagcc atgccaatct tcagtttatc tacaagttct
    55921 tccggtacaa catagtcata tattttatca aatttgggta caggtaaatc aactattact
    55981 tcagcaaatt tattcattat tattccttct tttacttatt tctcttaaag caaaaatacc
    56041 gccgattacc aggattgtat aaaaggtaaa tagtctccag ccaaaggtga ccaaccctac
    56101 aatatcactg ggaataaagg ccacaaacaa agaggcgaaa ccaatttctg ctacaccact
    56161 tgcccctgga gttggtgaat aaggcagcat cagataaaaa attgtctgca ttatataagc
    56221 ctggagaaaa tggggttcag ccccaaagga cattaaaact acaggcatga tcataaacat
    56281 actgctccag aaaacaatgg tataaaaggc gccccaaaat aagctctttt tgttttttct
    56341 taagatacca aacgaccttc taaagtctct taattctctt ctgcttttaa ccaaccatct
    56401 ttttgcttta taactttttt taataaaatt atttatcttg ttgatattca ggacataatc
    56461 tgttaattta tccccaattt taggtattaa ggttaataaa attattccta cagagattaa
    56521 aacacccatt ccaaatgcta aataaaatat ataatcgggt agtactcccg ccgaaatata
    56581 ttcattaaaa aatatcaaaa aaatgggggt taaaatacca aagtaaaata atcttaataa
    56641 aaaattagta acaactgctg tagaagcttt gcccacatta actcccttct tatggagaaa
    56701 atatatttga aatggtccgc ccccacttgc aaaaggagta acattagaaa cgaaagcacc
    56761 tgaaatatgg ataatcatac agtcctttaa actcaatttt ttatccacag atgagatcat
    56821 tactctcaat ctctggccag ccgaggcaat attaattaag attaacccca taattatcca
    56881 gagatatttc atatccatac ttttaagtac tctctccaaa gtatcttcat caattgtaat
    56941 tactataata atagcagaga tcaataaact taataccact gccattctta aacccttaaa
    57001 tacggtctgt cgatctattc tactttcttg tgtttgcata atttccacct cttataggca
    57061 aggcaaaacc gaaggaaaca actcctccgg taaaaatata ttttatactt tatttttaat
    57121 aatttctact ttatctaact cttcccaggg aagttctaaa tccaatctac caaaatgacc
    57181 ataatttgtg atctggctgt agattggtct cattaaatca aatttatcaa ttatgccgcc
    57241 cggagttaaa acaaaattgt ccttaatgac cttaactagt ctatcttcac ttactttgga
    57301 agtattaaat gtctccacca tcacagataa aggatgggct actccgatag cataagagag
    57361 ttgtacttca catttttcag ctaaaccggc tgccacaata tttttagcta tataacgggc
    57421 agcataggaa gcagatctat ccactttggt gggatcttta cctgaaaaag caccgccacc
    57481 atgacgggca gtaccaccat aagtatcaac aattatctta cgtccagtta aacctgcatc
    57541 accatgtggt ccaccgatta caaatctacc ggtcggattt atcaatatct cagtttgttt
    57601 atccagcata tcagccggaa ttatcttttt aatcacaaat tcacagagat ctctttttat
    57661 ggtctcctga gctacttccg gatggtgttg ggctgagatt aatactttat caaccctttt
    57721 cggtttatca tcttcatact ctatagtgac ctgggtttta ccatcaggtc ttaagtaatc
    57781 aactatatct tttttccgaa ctaaggctaa gcgttgggct aatttatggg ccagggtgat
    57841 tggtaaaggc atatattctt cggtttcatt tgttgcatat ccgtacatga tgccctggtc
    57901 accggcccca atctgttcct cgtctctacc ttctttggac tcaatagaat ggttgacccc
    57961 taaagcaata tcagcggact gctctttaat agaagtaatc acaccacagg tctctccatc
    58021 aaaaccatat tttgctctat cataaccgat atccatgatt gtctgtctgg ttatatcggt
    58081 aacatctaca taacaatctg ttgttatctc accggaaata aaggccaggc ctgttgttac
    58141 aaaggtttca caggcgaccc ttgcatcctt gtcgttcttt aagatttcgt ctaaaatact
    58201 atcagatatc ttatctgcta ctttgtcggg atgaccttca gttacagatt ctgaagtcat
    58261 caagtaattt tttgccatta taccaccttc atttatatat ttattaatac ttaaagttta
    58321 gcacatagat aaaactgtgt caattaagct aagagcctga aatatccaaa ctttttatca
    58381 aaactgaagc cgaaccaata tgtccactac tgggtagacc cattttgaag tcatcaccaa
    58441 taagttttat atcttttaac aactgtaaaa agttaccaga tattgtgatc tgttcaaccg
    58501 gtttatcaat attaccgtct tgtacataaa aaccgcgggc tgaaagggaa aaatcacctg
    58561 aaacctggtt tgcacctgag tgcagtccct gtacatcctc aataactaag ccttctttca
    58621 tttcttttaa tagttgttta aaagtatatt cccctttttg taagtacata ttggtgggag
    58681 aaatacttat cttgctttta tgagaggagc ggtaggcatt accggttgtt tccgtattac
    58741 tttttttagc agtttttaaa ttatggagat aggtggtaag ttcaccatct tttataacct
    58801 ctttatattt cgctgccgct ccctctgaat caaagggggt ccttctatac cctttttcat
    58861 aaaagggatc atcaactata ttaaccttag ctgctgccac cttttctcct aatttatctt
    58921 taaatagtga tagccctttt tgtacatttt cggctgagaa agttgaactg aaagttgata
    58981 aaatatcaat aaaaacttca tttcttagaa ttatagggta ggagcccgat tttactgttt
    59041 cggctccaaa ctgacttacg gcatccttca ctactttttg agcaaattta tccggatcaa
    59101 cttccttgat gtctctgaat aacttataat gggtagaagt gtgaacctcg cctttttctt
    59161 ccgctattat agaaatatag atataggcga tatcattttc ataagttagc tctaaatctt
    59221 tattatttaa aatatgttcc ccggtctttt catccgcata aatacagtag ttgatattcc
    59281 ttatcctgtc atctaaatca tatatctttt tttctatagt ttttaaaagt tcaatcttct
    59341 tttctctatc aaactcatag atgcccgtta aagccggtaa ctctttatac tttttatcac
    59401 cagcaaagat ctcttcttcc tcttcatcca ttatttcagc attactctta gcatttttta
    59461 aaactgtatc tattattgat tgatctattc gttctgtata ggaataaccc attttatcct
    59521 gatattcgcc tctaaaagct accccatact cttcattttc ttcataataa tcaacttcac
    59581 ctttatataa tctaaattcg aagttttcgg atttatttaa atatatctca ctattatcaa
    59641 attcattttg ctctgctctt tttaaaattt ctttcataaa tatatttaat tccattactc
    59701 atttccacct cttcccccaa cggttaattc cgaaactctg atagtcggct ggccgacacc
    59761 ggccggtaca ctaccactta aggaaccaca cataccttct gctaaatcta aattattgcc
    59821 aactagatct atctttttta agatatcggc cccttttcca attagggttg caccacgtac
    59881 cggtttttca atagagccat cttttacgat ataaccttct ctcacattaa aattaaactc
    59941 gccggtggca gtatttacag aaccaccgcc catatgtttt gcataaagac catactcagt
    60001 ttgagaaata atatcttcta aagaatgtcc accattatcg ataaatgtat ttgtcatccg
    60061 ggaagtgggt gaataacgga atgactggcg gcgcccggaa ccggtcggtt ccatctccag
    60121 tcgacggccg tttaaataat caatcatata accctttaaa actccctttt caatcagcac
    60181 attacgctgt gtagtatgcc cttcatcatc aaagttttgg gaaccccagg catttggtaa
    60241 ggtcccgtca tcaatagctg taaccttttc actggcaatt ttttctccca atttattagc
    60301 aaaaaccgaa ttattttttg caactgaggt tgcttctaag ccgtgtccac aagcttcatg
    60361 gaatattacg cccccaaatt tattatctat gataaccggc attttaccct gaggtgcatg
    60421 accggcttta atcattgtta ccgccatgcg ggccgcatct tcggcctgtt tttctacatt
    60481 tatttcatca aagaattcaa aacccatatg gccgcccggt cccaaataac cggtctgttt
    60541 ttcatcctgc aaagtggcta ctgcctggat accaagccta accctggttc tctcatcttc
    60601 aacaaagacc ccttctgaat tggcaatctg aacattttgg gtataatcag ttaaacccac
    60661 taccacctgt gtaattaact catcataacc atcagctgcc cgataagctt ttttgataaa
    60721 atttactttt tcaatttgat cgacatcggc cggcatgata gttatgggat gcaggttttt
    60781 ataatcaacg tactttaaat taatattatc ttttttagtt ttatcaaaca ttgaagcagc
    60841 ctttttggcc aacttcagca agttatcttt tttatcctta ttggtataaa catatatttc
//
LOCUS       DATGCJ010000061         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_409416, whole genome shotgun sequence.
ACCESSION   DATGCJ010000061 DATGCJ010000000
VERSION     DATGCJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409416"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VKN64_03270"
     CDS             complement(<1..559)
                     /locus_tag="VKN64_03270"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA59289.1"
                     /translation="MRSDIEIAQAAEMKPITDIAEKIGLTQDDLELYGDYKAKIKLDT
                     YQRLQSKEDSKLVLVTAITPTPAGEGKTTTTVGLGQALNQKGKDAMIAIREPSLGPTM
                     GVKGGAAGGGYSQVIPMEDINLHFTGDIHAIGIAHNLLSAAIDNHIKQGNELNIDKTK
                     VSWPRVVDMNDRALRDIVLGLGGTGN"
     gene            complement(592..2016)
                     /locus_tag="VKN64_03275"
     CDS             complement(592..2016)
                     /locus_tag="VKN64_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HMA59290.1"
                     /translation="MITRELVNNNIFTEGQLDKIHNATVEILQQNGIEILSEDARKLL
                     EENGAKVDEKQVFINEKMIEDALKSAPKKFTLHARNEENNVEIGGKNTVLAPGYGAPF
                     VMDFDEGKRRRAVYDDYIKFTKLAGNSNFIDVVGGVLVEPTDIEDELRHVLMLKASAK
                     YTDKCLMGSAMGAKKAKESIEMGSILFGGMDFVKDHPIMITLINTNSPLQIDSRMSDA
                     LMVHSKHNQPIVVASLSMTGTTAPTTIAGALVQQNAEILTGIVLSQLINPGAPVIYGS
                     ASSVVDLKTGNLAIGNPETAKMFNGTAQIARYYGLPSRGGGALTDSLKPDAQAGYESM
                     MNFMSAVKSGFNFILHSAGLLENYMTMCFEKFLIDDEIVGIVDNYEAGIKVNEDELAK
                     EVIMHVGSSGNFISEQHTYDHMRDLREPILSAKDRYFSNKEQPNTAERANKMYKEILE
                     EYEAPPLDKEVEKKLDQYIENIKE"
     gene            complement(2044..2892)
                     /locus_tag="VKN64_03280"
     CDS             complement(2044..2892)
                     /locus_tag="VKN64_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HMA59291.1"
                     /translation="MEKTDMLPHKAEIIEIKQETETNLNIKTFRCRFLDEDVQEEFDF
                     KPGQFMMVTVFGVGEAPFGFASSPTEEDYVEFSVKEVGLVTEALHNMKVGDVIGIRGP
                     FGNGFPVESTDGKNIIFIGGGIGLAPLRSLINYVMDEENRDKYEKIQLLTAFRSPEDT
                     LYCYDYDWWEDVENTRIKYTIDEPCEGWSKCVGYPHTLIEDKLEWDLPNTRFFTCGPP
                     IMIKFVTNRLKDLGIEPSEIVTTLEMRMSCGVGKCGRCNIGHYYVCKDGPVFTMEQLG
                     EMPDEY"
     gene            complement(2879..3919)
                     /locus_tag="VKN64_03285"
     CDS             complement(2879..3919)
                     /locus_tag="VKN64_03285"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA59292.1"
                     /translation="MMVKKVNKDGFKELFNKLKEEYNIAAPILMEKGKKELIEYGYVD
                     TFEDIIWDQQPDSSPKNIVFPQNSYLGNDFEGHAAYDDGQPTIILGVRSCDIEAIKVL
                     DQVFIEDEMYVDENYKNRRDKTIIVGLSCPERQSSCFCDTLGLDPVESDYAPIYLFKD
                     DGNFWIKVKDSQYEDLFNGLEEGDQDKLNKLLEERRAKFKESSFELDIPIPLPEKEMF
                     EADIWQEMTEKCLGCGVCTYYCPTCYCFNFFWEGEDGESQKYKSWDSCMFSSYSVHAS
                     GHNPRDSKDKRYRNRIMHKFSYHPLNYEGLACVGCGRCIDRCPVNLDIREALKMTEEY
                     LLEKGGAESGKD"
     gene            complement(3934..>4282)
                     /locus_tag="VKN64_03290"
     CDS             complement(3934..>4282)
                     /locus_tag="VKN64_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA59293.1"
                     /translation="ERCTRCYACREACYVCYCEQCFTESNQPDWVGESVKLNDNFTYH
                     LMRSMHMAGRCVNCGACEMACPEGIDVRALAAKMERAADEIFDYKAGLDPEQKTLLSE
                     YDVNDREKGFMDE"
BASE COUNT         1157 a          987 c          708 g         1430 t
ORIGIN      
        1 cattaccggt accacctaag cccaagacaa tatctcttag ggcgcgatca ttcatgtcaa
       61 caactctcgg ccagctgacc tttgtcttat caatatttag ttcattgccc tgcttgatat
      121 ggttatcaat agctgctgaa agtaggttat gtgctattcc tatcgcatgg atatcaccgg
      181 tgaaatgaag gttaatatct tccatcggga ttacctggga atagccgccg ccggcagccc
      241 ctcctttaac acccatagtc ggtcctaaag agggctcacg aattgctatc atagcatctt
      301 tacctttttg atttaaagcc tgtcctagac caacagtggt agtagttttt ccttcaccgg
      361 ctggtgtagg agttatggcg gtaactaata ctaatttgct gtcttctttg gactggagtc
      421 tctggtaagt atcaagttta atcttggcct tataatcacc gtaaagttct aagtcatcct
      481 gtgttaaacc gattttttct gcaatgtcag taataggctt catttctgcc gcctgagcaa
      541 tttcaatatc acttctcaaa actatcacac tccttataat ttttatatca tttattcttt
      601 gatattttca atatattgat ctaacttttt ttccacctct ttatcaagag ggggagcttc
      661 atattcttct aagatctctt tatacatctt gttagcccgt tcagctgtat tgggctgctc
      721 cttattacta aagtacctat ccttagcact taaaatcggt tctcttaagt cccgcatgtg
      781 atcataagta tgctgttcag aaataaagtt gccgctgctg ccgacatgca tgataacttc
      841 tttagcaagt tcatcttcat taaccttaat accagcctca tagttgtcta caataccgac
      901 tatttcatca tcaattaaga acttctcaaa gcacattgtc atatagtttt ctaatagtcc
      961 ggctgagtgc aagatgaagt taaaaccgct ttttacagcc gacataaagt tcatcataga
     1021 ttcataacca gcctgagcat ctggttttaa tgaatcagtt aaagctcctc ctccacgaga
     1081 aggtagtcca tagtatctgg caatctgggc tgtaccattg aacatcttgg ctgtttcggg
     1141 attaccgatt gctaagttac cggtttttag atctactaca ctggaggctg aaccataaat
     1201 taccggtgca ccgggattaa tcagttgtga taatactatg cctgttaata tttctgcatt
     1261 ctgttgaact aaagctccag ctattgtggt gggagcagta gttccggtca tacttaagga
     1321 tgcaactaca atcggttggt tatgtttaga gtgaaccatc aaggcatctg acatccggct
     1381 gtcaatctgc agcggtgagt tggtattgat taatgttatc atgatgggat gatctttaac
     1441 aaagtccatt cccccaaaga gaattgaacc catctcaata ctttctttag cttttttagc
     1501 gcccatggca gatcccatta ggcacttatc ggtatattta gcagaagcct ttaacatcaa
     1561 cacatgtctt aactcatctt ctatatcggt cggttctact aaaacacccc ctacaacatc
     1621 tataaaatta ctgttccccg ctaattttgt aaacttaata tagtcatcat agactgctct
     1681 tcttctttta ccttcatcaa aatccattac aaatggtgcc ccataaccgg gggccagtac
     1741 ggtattttta cctccgattt cgacattatt ttcttcattc ctggcatgta aggtaaactt
     1801 cttaggtgca ctttttaagg catcctcaat catcttttca ttaatgaaga cctgtttttc
     1861 atctacctta gcaccatttt cttctaatag tttgcgagca tcttctgaga gtatttcgat
     1921 tccgttctgt tgtaatattt ctacagtggc attatgtatt ttatctaact gcccttcagt
     1981 aaaaatatta ttattaacta actctcttgt aatcaatata cttccctcct caatcctgac
     2041 tgactaatat tcgtccggca tctcacctaa ctgttccatg gtaaagaccg gaccgtcttt
     2101 acatacatag taatgaccga tattacagcg tccacattta ccaacaccac aggacattct
     2161 catctctaag gttgttacta tttcagaggg ttcaattcca agatccttta atcgattggt
     2221 tacgaacttg atcatgatgg ggggaccaca ggtgaaaaac cttgtattag gtaagtccca
     2281 ctctaattta tcttcaatta aagtatgggg ataacctaca catttactcc atccttcaca
     2341 gggctcatcg atcgtatact tgattctggt attttcaacg tcttcccacc agtcatagtc
     2401 gtaacaataa agggtgtctt ccggagaacg gaaggcggtt aaaagctgta ttttttcata
     2461 tttatcccta ttttcttcat ccattacata attaattaaa gaccttagag gagctaaacc
     2521 gataccacct cctataaaga tgatattttt gccgtctgtt gattcgaccg gaaaaccatt
     2581 gccaaatggt ccccggatcc ctattacatc acccaccttc atgttgtgga gggcttctgt
     2641 aacaaggcca acttctttca ctgaaaattc aacatagtct tcttcagttg gtgaagatgc
     2701 aaaaccaaaa ggggcttctc ccacgccaaa tactgtaacc atcatgaatt ggcctggttt
     2761 aaaatcaaat tcttcttgta catcttcatc tagaaatcgg catctaaatg ttttaatatt
     2821 caagttggtt tctgtttctt gtttgatctc aattatttct gctttatgag gcagcatatc
     2881 agtcttttcc actttcagcc cctccttttt caagtaaata ttcttctgtc atcttaagtg
     2941 cttctctgat gtctaaatta acaggacatc tatcaataca cctaccacag cctacacagg
     3001 ctaaaccctc ataatttaaa ggatgatatg agaatttatg cataatccgg ttgcgatatc
     3061 ttttatcttt actatcgcgg ggattatggc cggaggcgtg cacactatag gatgagaaca
     3121 tacaggagtc ccagctttta tacttttgcg actcgccatc ttctccttcc cagaagaagt
     3181 taaaacaata acaggtcggg cagtaatatg tacagacccc acaacctaag catttctctg
     3241 tcatctcctg ccagatatct gcttcaaaca tttctttttc tggtaaaggg atgggaatat
     3301 caagttcaaa actactttct ttaaatttgg ctcttctttc ttccagtagt ttatttaatt
     3361 tatcttgatc tccctcttct aaaccattga atagatcttc atactgtgag tccttgacct
     3421 tgatccagaa attaccatca tctttgaaaa ggtagattgg cgcataatca ctttcaaccg
     3481 gatctaggcc aagcgtgtca cagaaacagc tggactgccg ttctggacag gatagtccca
     3541 ctataatggt tttatcccga cggtttttat aattttcatc tacatacatc tcatcttcaa
     3601 taaatacctg atctaaaact ttaatggcct caatatcaca gctcctaact cctaatatga
     3661 tggttggctg accatcatca taggcagcat ggccttcaaa gtcattacca agataactgt
     3721 tttggggaaa tacgatattt ttgggtgaac tatctggttg ttgatcccag atgatatcct
     3781 caaaagtatc tacatagccg tactcgatta gttctttttt acccttttcc attaaaatgg
     3841 gggctgcaat attgtactct tcttttaatt tattaaataa ctctttaaaa ccatctttat
     3901 taactttttt taccatcatc ctttttcacc tcctcattca tccataaatc ccttttctct
     3961 atcatttaca tcatattctg atagaagggt tttttgctct gggtctagcc ctgctttgta
     4021 atcaaatatt tcatcggcag ctctctccat cttagctgcc agggccctta catcaatacc
     4081 ttccggacag gccatctcac aggccccaca gtttacacag cggcccgcca tatgcatgga
     4141 acgcattaga tggtaggtga agttatcatt caacttaaca ctttcgccca cccagtccgg
     4201 ctggttgctt tcagtaaagc actgttcaca gtagcaaaca taacaggctt cacggcaggc
     4261 ataacagcgt gtacatcttt ca
//
LOCUS       DATGCJ010000062        50559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_97547, whole genome shotgun sequence.
ACCESSION   DATGCJ010000062 DATGCJ010000000
VERSION     DATGCJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 50559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50559
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97547"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1842)
                     /gene="glgB"
                     /locus_tag="VKN64_03295"
     CDS             complement(<1..1842)
                     /gene="glgB"
                     /locus_tag="VKN64_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HMA59294.1"
                     /translation="MSKTYISDYDRYLFNRGEHYQIYKFMGAQLKIENNQKGVRFSVW
                     APSARQVAVVGDFNGWNNFANQMEKVGDSGIWTTFIPGLTEGTIYKYSIKGFHGDTFL
                     KSDPVAFYSEKSPKTASVVASLDKYEWKDNLWMNKRKEKKPHQEPISIYEVHAGSWKT
                     DKEEDPYNYRRLADELIPYVEEMGYTHIELMPLTEYPFGGSWGYQTTGYFSLTSRYGK
                     PEDFMYFVDKAHRFGISVIMDWVPSHFCKDAHGLRLFDGTPLYESEDPKRAENKQWDT
                     LNFDYGQPEVWSFLISSARFFFDVYHIDGLRVDAVSNMLYLDYNKKDGEWTANEYGGN
                     ENLEAVDFIKQLNQVVFKDFPGIMMVAEESTAWPMVTGPVDKGGLGFNFKWNMGWMND
                     VLKYMEEDPLMRKGRHNLLTFSMMYAYSENYILPLSHDEVVHGKKSLLDKMAGDYWQK
                     FANLRLLYAYMFAHPGKNLLFMGGEFGQFIEWNYKQELDWLLLDYPKHQEIKRYVKDL
                     NEFYKRNNALYVKDYEHEGFEWIEADDYKQSVISFTRSGKKADDLRVILCNFTPVPRE
                     DYKIGVPLEGRYKEVFNSDQEKYGGSGILNEEPIKSKKEELHGFSQSI"
     gene            2034..3698
                     /gene="glgA"
                     /locus_tag="VKN64_03300"
     CDS             2034..3698
                     /gene="glgA"
                     /locus_tag="VKN64_03300"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HMA59295.1"
                     /translation="MSDKLKILFVSPEVSPFIKTGGLADVAGALPRELKKKGHDVRVV
                     LPEYKKIPDRFVMDFEHVTDFRMKMVWRDKYVGVNKLVYKDVPTYFIDNKYYFFRESL
                     YENGDKAEQYAFFSRAVLEMINKIDFKPDIIHCNDWQTGPLSLMLKDNYQIYDFYRDI
                     RTVFSIHNLAYQGKFDPAVVGDVLGVSNYHLTSGNIRHNDMVNYMKAGIMYSDVINTV
                     SKTYAEEIKNEYYGEGLDYILRMRDNDLYGILNGIDYDEFNPATDNRIYYNYDKNHLD
                     NKYKNKVALQKDMNLKPDKEVPVISIITRLVEQKGIDLIPPIVDDLMQEDLQFIILGT
                     GQPQYEDFFRYISSRYPDKISANIKYDAKLAQKIYAGSDMFLMPSRFEPCGLGQLISM
                     RYGTLPIVKETGGLKDTVLPYNEYEETGYGFTFSNYNAHDMLHTIKRALAFYNDEEVW
                     EKLVKRVMQQDFSWKKSAQKYEDLYSKLVIDKKVKTKPKTSGKVDINKANIKELITLN
                     GIGKSYANRIVDYRNKKGEFKAKKELMNIKGIGESKYNKIKNKIRI"
     gene            3714..4583
                     /locus_tag="VKN64_03305"
     CDS             3714..4583
                     /locus_tag="VKN64_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMA59296.1"
                     /translation="MPDLWTHIIGGDMVINDLNDRALCNVILENREHFNMGTQGPDFF
                     FYNDFWPWLKKKRGPRIGSLIHTEKPGDFVLAIFEVLREKEGAIQYPKALSYFMGFIT
                     HMIMDDKFHRIINERTYTDQEHKRFELELDCVLVKNYYNQDCHKINPISYIKIGEDMD
                     DLIKDIYYLNITELYDESINFEVINKSYQDMLKAQRILYSPFKVKAALLNLLNNFLSL
                     ELKQYLYANVNSFYVLTQKDVDDIEREFPDFITTSIDFMNEVSSYLKGEKSRGELKEL
                     IDGYLEQGFLERR"
     gene            4586..6124
                     /locus_tag="VKN64_03310"
     CDS             4586..6124
                     /locus_tag="VKN64_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59297.1"
                     /translation="MKNKLSIVVVLSLIIFLSLTLVSSEAQAEKFNIALSGKAQTLDP
                     AFLQRSISEWPIMNSIFSGLVKYKPGTYEVVPDLAKDWEFSEDDTKITFYLKEGVKFH
                     KGYGEVTAGDVEFSFERIIDPDKGSPEAESFSKLEDVKVIDNYTVQLILSEPMARLFT
                     STLPYNAGLIVSKQAVQEMGREEFSSNPIGSGPYQFEEWKIDSSIVLKRFEDYFGQPA
                     YIEEVEFIPMAEQMSQELALRSGEIDFGEVSLENYQQIQENEDLATETYPDLAFQWVA
                     FNVQKAPMDNPHLREALRYIIDPAEILIGAFADQADLAYSWLLPDMLGYKKLSHKRLD
                     EVGRDKILDLLEKAGYPNGEGLTLKYVTDANDIRKNIATLVQAQLAEFNIQLQIEAME
                     IGPKIEAWHNGEYHITYARFGNTVDPGYCSQWFLSRQVGNWNLMHWSNEEYDQLWEQA
                     EKTMNEDERAKLYQKMQTIVDEADIAVWVTHGVKTPTWNDDYEVTLTPSGGILSWLVQ
                     KKSN"
     gene            6139..7077
                     /locus_tag="VKN64_03315"
     CDS             6139..7077
                     /locus_tag="VKN64_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59298.1"
                     /translation="MLIYTVKRLLLLIPTLLAVMLLLFSLSYLLPGEPAEIILGPRAS
                     QQMIKDLNQRLGLDKPWYVSFYLYVKQVLSGDLGTSIFRDVPVLQAVLGVLPYTLALT
                     LSGMGIAVILGITIGLTAANYQNTIWDGILEVFSFICASTPTYVAGVIILLLFSIRLD
                     FIPVMGAGEGSILDFLHHLIGPALALSVGWTGYIARLTRSSMLETINEDYITTSRSFG
                     LPKTFIYYRYALKKAIKPIVAVIGLGIGRLLGGALFVEVIFTRPGLGRLTVDAIFERD
                     LPMVRGGVLVAAFLFVIANLLADLSYAYLDPRIRNE"
     gene            7087..7956
                     /locus_tag="VKN64_03320"
     CDS             7087..7956
                     /locus_tag="VKN64_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59299.1"
                     /translation="MNKYIYIKKKIKWVYERMIGDYRFYIAGVIIFLFFLMAIFGPQI
                     APHDPYKTDVRSRLEAPSLTHPAGTDSLGRDTLSRLIYAARPAFYVAFGGLFISIIVG
                     MILGSIAAYSGGILDNIIIFLFDVIRAFPPIILILVLVAIVGPSLQSLIIILGVTIMP
                     RYGRVVRAETLSVKEEEYVSVAKSMGASSFKIISFHIIPNIFASVLVLAGMDVASMIM
                     WEAGLSFLGLGVQPPKTSWGVMLQSGYQYIEKAPMMLVAPAVAIMLTMMGFSLLTESL
                     RNALNPKSEQKEG"
     gene            7959..8948
                     /locus_tag="VKN64_03325"
     CDS             7959..8948
                     /locus_tag="VKN64_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59300.1"
                     /translation="MAENLLEIEKLKIIYKRREKRFLALDCVNLDLKEGEVLGVVGES
                     GSGKSTLGLSIMRLLPPQARIKNGEIFFKGKNICKINEKKFNREMRGKEITYIPQNPQ
                     SSLNPVFKVGRQLKDIIKYRAQENFQGQDINKAAVDIMTKMGIADAEKRLDEYPHQFS
                     GGMKQRVLLSMAFVTNPALLIADEPTTALDVTVEAQILGLLADLIKEYQSSIIYITHD
                     LGVVSEIADRVAVFYAGRVIELNNRYNIFNNPKHPYTEALINCLPGDKQSEKLNVIPG
                     EIPDLSDLPGGCNFHPRCKYAKERCKNEKPEFFELENGGRTACFYHEEVGRYQ"
     gene            8945..9925
                     /locus_tag="VKN64_03330"
     CDS             8945..9925
                     /locus_tag="VKN64_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59301.1"
                     /translation="MNIIEVRDLKKYFYKKSGVLHKVFGVGPQVIQAVSKVSFNIEKG
                     NTMALVGESGCGKTTVARLILNLIEKEAGQIYFKGKALDEMSEEEYNDYRENAAFIAQ
                     DFRSSLNPRKLVGEILAEPFRIHPGVIDKEREEARIKKMVDLVGLNEEALLKFPHEFS
                     GGQARRIGVARALMLNPEFIVCDEPTSGLDLSISAAIINLMKELKEEFDLTYLWISHN
                     LNVVRYISDYVGVMYLGRIIEIGKTKEVFNDPSHPYSEALIESVPSFSKEKKKIKIKT
                     LEGEVPSPIDPPPGCPFHPRCKYSQAKCKEVVPILENKGNNREVACHFPL"
     gene            10001..11149
                     /locus_tag="VKN64_03335"
     CDS             10001..11149
                     /locus_tag="VKN64_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59302.1"
                     /translation="MKRSFISLFLLVILLFAMSSLVMAQDVVKLGFFAPVTGPAAADG
                     ESALNSAKLAVEVINEEGGIDGREVQLVEYDDHLDSKQSVSIAQKLTTRDNVVAVVSG
                     SYSGTTRPAATIYQNAKVPMISAYAIHPDIPKTGDYIFQQSFPGPVQGRVGGYMAVDD
                     LGAQKIAILYVDNDFGSTLNDNFQEYVKEKGAEVVYVDSFAIGEREFNPVLTKIKGLN
                     PDLIYVVAYAGEGAAIMRQSVDVGIEAQILGTEGMDSTTQFLQVAGEAAEGLIITTNL
                     NRDSEEEQVIDYINRYTEKYGFAPDMVGASTYDAFMLLAHVMRQVGPDSEAIKDAMYE
                     VEDYMTNTGLLYRYTEVGEAIKPVQVQVVKDGEFHYYDQITDMDIIAP"
     gene            11245..12111
                     /locus_tag="VKN64_03340"
     CDS             11245..12111
                     /locus_tag="VKN64_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA59303.1"
                     /translation="MFLQQLLNSIAIGSVYALTSMGATLIYGILGILDIANAGAYVLG
                     AYLGLLFYSLTGNIIISFAAAMATTGLLGILIQKYVYRPLLSQPAYIPLIASIGIFIF
                     IENVIRLIAGPTIKQFTVKLPFSNLDYANLHINNVWVLIFIVALILMFLLWYLLKMTK
                     IGLAWRATSQDLDIAKTMGINTNKVISLNFFLGYSFAASAGILIGILYNSIYPTMGSV
                     VSYKMLAIIVLGGLGNPFGTVIAALLIGFIETMVGAYIGFFIPRNAIAFVALVIILLI
                     SPKGLFTQKSRI"
     gene            12130..13017
                     /locus_tag="VKN64_03345"
     CDS             12130..13017
                     /locus_tag="VKN64_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA59304.1"
                     /translation="MYAIVVMINVGIFVLLALSLNIITGYAGQPNIGQGAFFGIGAYT
                     GAVLTGKFGIYFWLAMPFAAIITGFIGFFLGLISIRIKEDFLAIVTIALNFIVAAVFQ
                     NVEFFGAAMGLSVPPPNWFGEPLSYQGYLTIVIILIILVVIFSKWIEKSWLGMALGAI
                     RNNEMAADSLGIDTKKFKIMAFVIGTSIAGLTGVVYAHYMTFIMASDFGFLTSVTILS
                     MVILGGNNTIRGPIIGAVILGLIPEVFRFISDYRLLVYAGILILMIRFQPSGLLGDDS
                     FVWNKLKLLKASLGRRRGA"
     gene            13019..13777
                     /locus_tag="VKN64_03350"
     CDS             13019..13777
                     /locus_tag="VKN64_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59305.1"
                     /translation="MENILKIKNVEKHFGGLIALHDVSFDVKKNEITGLIGPNGAGKT
                     TLFNTLSGVYKANGGNIFFKESDIINLPPYKIADIGIARTFQIARPFLDLTVSQNVLV
                     GLGEKNYQGFLKPFKLSINAVNIKKAHDILEMVGMKEFRKNEAADLSLGYKRRLGIAR
                     ALALEPEVLMLDEPLAGLGKDVSEKMLDLVEDLRKEGMTIIIIEHDMEAIMSICDKIV
                     VLNQGEKIAEGKPKEIQNNHDVIEAYLGKDELNA"
     gene            13770..14474
                     /locus_tag="VKN64_03355"
     CDS             13770..14474
                     /locus_tag="VKN64_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59306.1"
                     /translation="MLEVKNINVFYGESQALYDVSLKVEAGEFVTIIGANGAGKTTIM
                     NTIMGLIKPKSGQIIYKDKDLTKINPWERTDLGISYVPEGRRIFPDLTVMENLRVGAY
                     RLKDDSQLASNFDYVFDLFPRLADRKNQLAETMSGGEQQMLAIARALMLSPELILIDE
                     VSMGLMPILVNKVFDVIERLNEQGITVLLVEQNARKALNYADRGYLLEVGNIVKSDTC
                     ENLKCDEMIKKAYLGG"
     gene            14477..15064
                     /locus_tag="VKN64_03360"
     CDS             14477..15064
                     /locus_tag="VKN64_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMA59307.1"
                     /translation="MCGRYFLNIALDELENRYGITDIPVEIKEHQGEIFPSNEAPVVF
                     NENKQKSVGLMNWGFRPSYSNNLVINARSETVHKKNLFKESFHRRRCIIPASGFYEWE
                     KVADKKIKHFINIQEDEIISLAGIYDFFNINGKQQMAFCILTKDAPQELINIHDRIPV
                     IIKKEYENEWLENKFTPGIFEYLQPKGFNFQVEAL"
     gene            15194..15928
                     /locus_tag="VKN64_03365"
     CDS             15194..15928
                     /locus_tag="VKN64_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA59308.1"
                     /translation="MKKLLLTVLTLTLIFSLSAVGLAQTYTVGTSAGFPPFEYVEDGE
                     IVGFDIDLMKAIAEEMDFEVEFRDIAFDSLPSAVKTGNIDVIAAGMTITEEREKALDF
                     TESYYSANQSIIVQEDSEMNISVLYGDHDISVQTGTTGDLWVTENLKDQGILTGDVQR
                     YDTFVLCVSDLVNGNVDAVVLDEPVAERYAEMRPVKIVGVIVTGENYGLAVNEGNTEL
                     LETLNEGLRRVRESGKMDELVAKYFQ"
     gene            15987..16643
                     /locus_tag="VKN64_03370"
     CDS             15987..16643
                     /locus_tag="VKN64_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HMA59309.1"
                     /translation="MSSLPNLLEGALVTIQLTLLSLLIGLVLAIPIAFGQIYGPKWLR
                     KLIGVYERVVRSIPLLVILFLIFYGFPRIGIKFPAFTAAVLGIGIRSSAYQSQIFRGA
                     IQSISGSQMKAALSLGMTKIQSFKSIILPQSIRIALPPMANESAIVLKDTSLAYALGV
                     IELLRQGGYIIATEYAPLPIYLTVAAIYLVMTLTINTTLGHFENRLKIPGIGIEERVR
                     "
     gene            16640..17401
                     /locus_tag="VKN64_03375"
     CDS             16640..17401
                     /locus_tag="VKN64_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HMA59310.1"
                     /translation="MSEEKRTILKIEDLHKSFGNNEVLKGVSLELKQGENKVIIGPSG
                     TGKSTLLNNVNRLVPPDQGKIYLDGELITPENINRMRQEIGFVFQDFGLFNHLTALGN
                     VRVGLIKVKKMNKKDATELAMEELRRVGLEENADQYPAELSGGQKQRVGIARALAMQP
                     KLILFDEPTSALDPELIGEVLNVMKDLAQEGMTMLVVTHEMGFARTVSDEIIFMEHGN
                     IVEQGPPKQLFNNPEVERTREFLFKLTELYGEGDE"
     gene            17407..18069
                     /locus_tag="VKN64_03380"
     CDS             17407..18069
                     /locus_tag="VKN64_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HMA59311.1"
                     /translation="MYEVFVGAWPHLFEGVQLTLLLTVISIITGTIIAIFLSLGKTYG
                     PKYVRWPINVFIELIRGTPLLAQLFILYFGLPPYGIKLSGFLVAYIGFTMNCSAYTAE
                     YIRGSIMSIESNQLRVAESLGMTKWQGIFNVILPQALRRVVPSWTNEFIYLLKYTSLA
                     YMIGTHELMTQAKFFASRNYEFFKVYLIVALFYLVIVVFFTQIFSWVESKFTIPGFEV
                     EH"
     gene            18284..20317
                     /locus_tag="VKN64_03385"
     CDS             18284..20317
                     /locus_tag="VKN64_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMA59312.1"
                     /translation="MTKNIEIIYELFNLLENNEYKKILKRICEHLDVERAFLLNYYEN
                     YNTTDMVIGWNMSDEIHDYLKNTDIEEFTQTKKQLAQGKPIYISNISQENNYPRLVEL
                     LRSYNQNSAVLVPIRDADFELIGIMGIASENTLLCEEKEDCQKILEILTYNLKYNWED
                     EILKLKYRSLIQNSASAIVILNDEGNIVELNKKFEKLANSPKTELKNQKCFFDFIAPR
                     DKEDMIKRHNERRKGKEVVSDYDFNFIDSDNRKKEVNVKVKMIPGTDKSVCSLVDITQ
                     KKRTENLLKLQEEKLSESYNQLKQSRDQLESTYLDLIDKNIENERLLNNIEKLIDLMS
                     SLHMSNGNNLNNFLAELLYTALEIVDVADYGSVYIFNENDCIEYIETVGHNLKKLNQI
                     EIEGEPFRHNNKEVEIVDNILDKNKEYMSDYIIQKIKDASLPIKQSIPVTLQEGNNIF
                     GGICIDIARGSKKEFSRDDKRLITSFKNLANTFFKLERYNRLNSQFNKELVGSLLNFL
                     NQYDLYTNNHSENVAQLSLEIAEKVGLSKEEKNNTYWTGMLHDMGKLIIPARILNKEG
                     LLTKEEYAVIKEHPTLCYNALKDSDFLKDIAQYVLHHHESWDGSGYPAGLQGEEIPLI
                     SRIIAAADAWDAMRSERAYRSSLSKEEAKKEIKINQGIQFDPDIAELLLEIVE"
     gene            20342..21871
                     /locus_tag="VKN64_03390"
     CDS             20342..21871
                     /locus_tag="VKN64_03390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMA59313.1"
                     /translation="MNNILLNILLVIFLVYLFNKLKTKYSDSLDMPNYLSYENLFDKL
                     EVPMLLLGKENEVVRYNDSFIDQFDLDEKEIEGEPFNSVFEIEGSETGFNHILEKFNS
                     GEKYKGKIMFFDKKDKRYECELKLYPIFENDELVGKHIIINQIDKEEEKISSALRESR
                     RKIRDLHQTALKMENVRTKKEVYDLTVKAAENILDFDLCTLDIVEGNKMVVKATSSNI
                     IPGESISSPLDVDSIATQVYKNKQALLSPDIQKEEFANPTSSKYHSALTIPIGERGIF
                     QAVSTKVNEFTREDLELVELLIAHALSALQRLEMDQEIRYLGFHDSLTGLYNRHYLNE
                     EINRLDTKRQLPISVIIGDLNGLKLINDIFGHEEGDKFLKKAAELLKDCVREEDILGR
                     WGGDEFMIILPKTSCQEADEILARINRKVQKTYKEKRPISISLGRATKDINFADSFNK
                     VIEEADYKMYKNKAIMHEEDNNPLVEMIKEKMQKKELKKGEVYRLDSLLNMFKNNPFE
                     R"
     gene            21990..22748
                     /locus_tag="VKN64_03395"
     CDS             21990..22748
                     /locus_tag="VKN64_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59314.1"
                     /translation="MQELLNIKELNLTLDKERILRNINLSLERGEVFAVIGPNGCGKS
                     SLAYTIMGLKGYTQKSGEINFKGEEISELSVTERARKGITLAWQEPARFEGVSVREFL
                     SIGARSRGREISEEDLNWALNEVAIVPKKYLDRNVDDTLSGGERKRIELASILLMKPD
                     LVILDEPDSGVDVVALNNIGDVIKDFKDENIGVLLITHSDEMLEVADKAALICGGEVF
                     NVGDPQEVSDYFKYECMPCEDENPEEHQEVYFRE"
     gene            22741..23679
                     /locus_tag="VKN64_03400"
     CDS             22741..23679
                     /locus_tag="VKN64_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HMA59315.1"
                     /translation="MNDYEMMVDAYKEAGGEDVFSDSDTAHMVLEQDKVLGKHEVEGL
                     IMEAEKIDDNTVDVKLRVKEGYKIKNPVHMCFGVLPREGLQKIIMDVEIEKDAEIKVM
                     SDCVFPNATKVKHVMEADIHLAENSKYEYEENHFHGDTGGVEVIAKADIIMDEGSELF
                     TLFTLRKGRVGKIDFDYESELAANTKVEMLTRISGYEDDKVKIREAAHLNGYNARGLL
                     DSKIAMVDEAQAEVYNELTASAPEAKGHVDCTEIIKNNAVARAIPIVEVKHPEAKVTH
                     EAAIGSVDSDQLQTLMARGLDEEEASDLIIQGLLKG"
     gene            23700..25292
                     /locus_tag="VKN64_03405"
     CDS             23700..25292
                     /locus_tag="VKN64_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMA59316.1"
                     /translation="MRRRPGITLIITFLFLLLFSFSLTVGAQDFYTICKEGTPAEIQQ
                     AIDNGAEVNQQNKEGITPMMTAARYNSNYKAVVTLYNNNAYVNARDNEGWNALMYAVR
                     YNDNIDVTRILLKMNSRVNIDDTEGTTPLILAVKYRNEKMVRLLLNYGANANKANNRG
                     ETPLMLAIQDDLNVEIIDALLLSGANVNIRDKQGRTPLYYVIDKGDYGLVRRFILFGA
                     KVKISDYDDFTPLMMAAKEAKNVKTLKILNIEDSNINAQDENGMTALMYAARYNNNQS
                     VMNYLIDEGARINATDNKGNNALTYAVRYNSYNIITLLLNRGASLQQENVAGKTPLFL
                     AVERKRDLQILSLLVSRGANVKDRDKFKGQTILMAAVVNYDNPAIINYLIDQGANPTA
                     RDFNDKRVVDYLEENNDLKGTDLYWRLNYLEPDKRRKSLLDFKDKTSVAFTSAVIPSA
                     GHYMVDSWWPKGTLFMVGEVGSLITALASDDEGTRGTALTVFGVLKVLEIVDVLNETE
                     KYNEDVEEYNKKAREFNEQLEN"
     gene            25334..26302
                     /locus_tag="VKN64_03410"
     CDS             25334..26302
                     /locus_tag="VKN64_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59317.1"
                     /translation="MKLAKGNITVVMLSLVLILTLLFGGVVNAAPKEVVIQTPPVPAA
                     LPLLRLQKTGELDNFLKFDVKVSPDHQRAISLISKNEIDFMVTGVNVGAKIYNKGIDV
                     KMVNVNTWAIDYLLTNGFKAESWSDLKGKTLSLPLKGGPLDFLARYLLKENGLDPQND
                     VNLVYRPLPNGAKYFMSGQVDSIILPEPLVSVSLTKSDKAQLSLDIQKEWGKLHNGDE
                     RIPFVGLFVSNQFVQEYPGLTDIIAGKYIEGVKYYNNNPEEAIKDASNYFNVPEPVMK
                     KAWSRVDLSLYPDSESRDLVNTYFNHILEMYPEMIGGNLPDEAFYY"
     gene            26283..27047
                     /locus_tag="VKN64_03415"
     CDS             26283..27047
                     /locus_tag="VKN64_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59318.1"
                     /translation="MKRFIINKFKKILLSTTGFLVVLLIWIYYSRQMNPLILPSPLQT
                     FNAFIDIYQSGNLFNNLFITIRRTIIGYGAALIIGFMIALILNKSNTLYTITRPIITV
                     IQTTPPVIWLALAVIWFGIADNLTPIFLIFIVTLPIIFVNIFEGLQDIDQNLIEMAEV
                     YKADRQEIFFDIYVPSLIPYIFSALSIGMAFAWKSTIFAEFLGSNSGVGFALSMANSN
                     LNTDKLFAWGIILILLMLAVEYLIIKPVQKKATRWK"
     gene            27049..27813
                     /locus_tag="VKN64_03420"
     CDS             27049..27813
                     /locus_tag="VKN64_03420"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59319.1"
                     /translation="MEKILEFKDIYKNFAELKVLKDVTLHVNKGEIVTLLGPSGSGKT
                     TLFKIAAGLLKQDQGQVMRADELRTGYVFQEPRLLPWRNIKDNFHFVQQNYLPKKKAA
                     SIRNSLLKLTNLEQTLDNYPAELSGGMKQRIEILKAISIKPDFLIMDEPLKSVDTQTE
                     VNLRNLLLRLHIERNIDMFIITHDPEEAALISDRIYVLSDKPGVIKKEFKIETKKEDR
                     TLKHDEIYSTIDEIINIFMDLVEDYQWNRDHVDSIE"
     gene            27885..29285
                     /gene="lpdA"
                     /locus_tag="VKN64_03425"
     CDS             27885..29285
                     /gene="lpdA"
                     /locus_tag="VKN64_03425"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMA59320.1"
                     /translation="MTEKKITIIGAGTGGYVAAIKAAQLGADVTLIEKENIGGTCLNW
                     GCIPTKALVYSAEKYSEMQEARRFGIEVNDVNLNMKRVIRNKDTIVKQLVGGVEFLLD
                     KHNVKVYKGVASLLDQNTVSVKTEDGREIIETDYIILATGSKVKKAPIKGIDMEGILT
                     SRDILDLEELPESLAVIGGGVIGMEFAFIYANFGVDIKVVEYLDDILPGVDQEVTSEL
                     GKVAKRAKIDITTGAQVKEVAKTDSGYQVKYLKDEENQTIEVDKVLVATGREPSFGGL
                     DLDKIGVEKENNAIKVDEHLKTSVDNIYAVGDVTGKILLAHVASHQGVVAVENIMGEE
                     KKMDYDAVPAAIFTKPEIATVGLTEEEVKEAGIDYNIGKFPFRANGKVKTMNKRNGFI
                     KIISKKDSGEILGSSIIGVHATDLIHELTLAVNKNMTIEDIIETIHAHPTTSEVIHEA
                     ALDSIGGALHYVRLNR"
     gene            29266..30291
                     /locus_tag="VKN64_03430"
     CDS             29266..30291
                     /locus_tag="VKN64_03430"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HMA59321.1"
                     /translation="MSDLIDKFREKIEHARIVHSDSFNPWYNLALEEYLLNNTKKNEV
                     ILYLWQNDNTVVIGRNQNAWKECNCRLLEDNDGKLARRLSGGGAVYHDLGNLNFTFVM
                     DKNLYDIHTQLEVILKAVRDSNIEAEFSGRNDLVASGRKFSGNAFYYSTNSSYHHGTI
                     LVDTDFKNMVEYLQVSQEKIQSKGIESVKSRVVNLQELNPDVTVEQMKSELEKYFKEL
                     YQVEPEPQTISPHQNEEIAKLQEKYSSWEWRYGQAPDFDITYENRFDWGDIQLGLSLK
                     KGTIEEAKIYSDAMNADLIENIADNLSDCQFKIDEVNKAIRNVEHDKKDEEVIDDLIV
                     WLKEKRI"
     gene            complement(30386..30601)
                     /locus_tag="VKN64_03435"
     CDS             complement(30386..30601)
                     /locus_tag="VKN64_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59322.1"
                     /translation="MSAKKCKACGATYHKDGRFYKCEECGDIYCHDCAQYHTKEEKAI
                     TESAKKGDKDGYVRAVCPSCEAEMYRY"
     gene            30773..31930
                     /gene="wecB"
                     /locus_tag="VKN64_03440"
     CDS             30773..31930
                     /gene="wecB"
                     /locus_tag="VKN64_03440"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMA59323.1"
                     /translation="MMEQLKVMTVFGTRPEAIKMAPLVKKLKKEDNINCIVTVTAQHR
                     EMLDQVLDLFEIKTDYDLDIMSNQQTLVDITVNVLKNLETVLNEEEPDLVLVHGDTTT
                     TFIGALSSFYKQIDIGHVEAGLRTGNKYSPYPEEMNRHLTGVLTDLNFAPTINNKDNL
                     LKENVDENKIFVTGNTVIDAIQEIVKENYKFDNEQLKDIDFDNNKVILLTAHRRENLG
                     EGLTGIFQAVNDIIKSNRDAEVVFPVHLNPKVRKTAYKHLEDNKKVHLIEPLGYLEFA
                     NLMNKSYLIMTDSGGIQEEAPGLGKPVLVLRDTTERQEAIQAGTVKKVGVDRVKIFEE
                     ADLLLNNKQAYLKMAEATNPYGDGKAVARIIDYILFHYNIKGVKPEPFQVD"
     gene            31932..32585
                     /locus_tag="VKN64_03445"
     CDS             31932..32585
                     /locus_tag="VKN64_03445"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMA59324.1"
                     /translation="MVNNDDFSKDREAMVKKQLIRRGIEDKLVLDAFRSVPREKFVPD
                     HVKEYSYHDGPLPIGEDQTISQPYIVAEMVEALELNRDSIVLEIGTGSGYEAAILAEV
                     AKHVYTVERIQSLADKAKKKLDELGYENITIEVGDGSLGWPSGDKVFDGIVVSAGAPS
                     IPNSYKNQLKTGGYLVIPVGSRALQNLYKVRKNQNGKMEKQSLGHVRFVPLLGEQAW"
     gene            32600..33265
                     /locus_tag="VKN64_03450"
     CDS             32600..33265
                     /locus_tag="VKN64_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59325.1"
                     /translation="MAENIKAKVLQNSVMVSGHPRSGTSITCQLVESAGVHFPSDYEG
                     DEYNVSGYYELEISKDLSKKLIEKAMTIKNTIKMNQVIEKLNENEGLSGLKLVRVPAI
                     FFYRHVADNLKAVFIFRNPADVKASMLRRGISSFEPDWFANNNALIAAYENIEDSILI
                     SYETLLSDKEKVKALFNKIDLEVDVSLIKTGQRSQKHSDIVLEDKEIKLYERLKELEA
                     KNF"
     gene            33280..33765
                     /locus_tag="VKN64_03455"
     CDS             33280..33765
                     /locus_tag="VKN64_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMA59326.1"
                     /translation="MDIYQFAINFEQENREYYEELAEKSASERIKNVFLELASEEAKH
                     ERIVKQLRDKEDVGDLEFDIIPKVNKVFEEIKADMPDNVMPKDQVDVYKKALNMEEKS
                     KDFYAEKAEEVEGEKAKSVLERLAKEEKKHENIVRSIIELVNRPNTWLEDAEWYHLED
                     Y"
     gene            33864..35558
                     /locus_tag="VKN64_03460"
     CDS             33864..35558
                     /locus_tag="VKN64_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMA59327.1"
                     /translation="MDFNILIGGEAGQGLKTVDNILGKILFREGFNIFSSKDFMSRIR
                     GGHNFMQLRISDEKLYGPDNDLDLLIALNKESVDIHREQLKDDGIILIEGDEEVIDGR
                     TILVPASVIANDINPKGINTVFVGAALKIMDLDLDKAKQVVKEYFDEDLVEDNIKLLE
                     RGYKAVDPVYEKLKENVSDKSEEVFIDGNSALGYGALTGGLRFYSAYPMSPSTGIMNF
                     LAGQQENFDIVVEQAEDELAALNMALGGSYSGIRSMSGTSGGGLALMNEAIGLAGITE
                     TPVVVADVQRPGPATGLPTRTGQGDLLFAINSAQDEFPLMVIAPRDQKDLFYNGFRAL
                     NIADKYQIPVIILSDQFNADSSKNIEEFHFDSLTINRYLISEDDPAAKDYKRYKFTED
                     GISPRAYPGQLKGETVLVDSDEHDEEGHIVEDGETRTKMVDKRARKLDKLIQEDLQEP
                     KYFGDEDIDYLLLTWGSSYGPTREAFQLLKEDGIKVGLLSFNDVWPLPKEQLEDLACD
                     GVDLITVELNSTAQFGTLIASETLLDVKQSILKYDGRPFTGKEIYNRFLDEVIDNG"
     gene            35551..36405
                     /locus_tag="VKN64_03465"
     CDS             35551..36405
                     /locus_tag="VKN64_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA59328.1"
                     /translation="MANNKEFGISSETAWCPGCGNFPLRKALSAALEEMELDVDQVAM
                     FTGIGQAAKMPHYINLNGFNGLHGRSFPPATGARVGNHKLKIIVESGDGCSYGEGGNH
                     ILHNIRRNPDVTHLVHNNQVYGLTKGQASPTTEEYMQTGIQVEGTTAKPFKPVKFAVG
                     MGASFVARSFVGDQDHLKEMIQAAMNHRGYALIDILQPCVSFNKINTYQWYSKRVYKL
                     DNNYDPEDIDQAFKKAREWGDNIPIGIIYKNEQKIFRDRLLLDDDQPLVEKDFDPQDA
                     KKIIDNFK"
     gene            36458..36982
                     /locus_tag="VKN64_03470"
     CDS             36458..36982
                     /locus_tag="VKN64_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HMA59329.1"
                     /translation="MDAKERREKLINKLIDENEPVTGANLADFFGVTRQVIVQDIALL
                     RAQGYDILATSQGYILKNNMGVKTYSKTIACKHDMGNVKEELMIIVKHGAKIKDVKVE
                     HPIYGEIAGMLMLQTSQDVENFLNNTEKFEASLLSSLTDGVHLHTIEAMNEDVLDEIV
                     KELKEKGFLLEEDL"
     gene            37059..37256
                     /locus_tag="VKN64_03475"
     CDS             37059..37256
                     /locus_tag="VKN64_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59330.1"
                     /translation="MNKEELNKKRLFWIIWLIVLLLSAYFVPFVLLRDVNQIEASFLY
                     WTIFAVLAIFSTIKITTYWSD"
     gene            37260..38735
                     /locus_tag="VKN64_03480"
     CDS             37260..38735
                     /locus_tag="VKN64_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HMA59331.1"
                     /translation="MNTFYVYLAVIIYVLIGFYIAYKARQGMKQGVIEYFLDNRSTSG
                     IVSALSYSATTYSAFMMIGLAGFTYSGGVGALGFELIYLAGLSLVAFFGPRFWLVGKK
                     YGYVTPYEMLGDRFQNKWIAIISAGAACVFLIPYLSVQLMGIGYLLEGLSGGSIPFLA
                     GVMIAAVLAISWAIIAGMRSVTWTDSLQSLIMILVSVLILFYVVYTQLGGFLSFFNQL
                     ETEYANFLSVPGPGFFKFQTFLGLTLPWFFFSISNPQVSQRLFIPNSLKAMKTMIKGF
                     LIFGLVYTLVSVLWGFSAKLLLPGLEQADLATPTLLGSEHVPMLLGLIAMIGITAAAI
                     STVDSIMLTLSSLIVRDLMQSRDEYSDKKQLRIGKAVIFVIAVIGIIFAAQQLDLIAT
                     LSVASSVGLMVVVPTIFGAFFWKNATAAGSLASIIIGAVAALYLQFAGLNPLGVHPGV
                     WTFIITVVTFILVSLFTNAPKEKAEEFMGYLDQELADKNIL"
     gene            38809..39864
                     /locus_tag="VKN64_03485"
     CDS             38809..39864
                     /locus_tag="VKN64_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMA59332.1"
                     /translation="MQYKDVSRDLEKKTNLLVDSTMKCVYGDKLKDIKGSDKEEKSIR
                     DIEYIINYLSEAAYFNNKGLFEDFSLWLNSLFNNIDLGEEVFKTTYKCIIKQIEHEFD
                     EKVSNYLNNIIRESMQEALNNKEEIASFIKKDNPHQEYAKRYLDLLLEHKKEQASKLI
                     TDEALDEMGIADIYLNIFQPVQKEVGRLWHANKVSVAKEHYISSVTQLIMSQLYPHFL
                     SIGGKKGSVVTTAVGNELHEIGIRMIADLLEVDGYDTIHLGANTPNTGIIQTLKSNKT
                     KLLGVSVTLPLHLKELDKLITMIRDERELNNLKIMIGGCAFNRNPELVNEFDVDEYSK
                     DAQDAVKKANILTGVVN"
     gene            39864..41228
                     /locus_tag="VKN64_03490"
     CDS             39864..41228
                     /locus_tag="VKN64_03490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMA59333.1"
                     /translation="MGFVVLTSPELKIKRILYNDTSFSFLKEDRILTSFVNKNERSKL
                     LDFVHKIQADKAEFGYEIHLQTKEDSILYNFSGIKAENDSILILASQFKEEIINQYDH
                     FMKVNNQYINKIRRLLKNQIKSDNEVVLSGDSSDIYNQISQINNELMEVQRELTKTNK
                     ELKWQKERYFATLKSIGEGVIALNEEKRIQFINEAAQAILGIKDNIKGDYLQAQDIKF
                     LDQNENDILENIIERVCNTGGLIKKEDLLMKTKEKEVPIDLTVSPIRINEEKLLGIVI
                     VITDITLKKKHEEKLRKLAVTDRLTNIMNRRMGIKYLEKQIERLKREDFPLTVCFIDV
                     NGLKKINDNYGHLEGDHLLKEVAEILDQNVRSSDVVARIGGDEFLIIFSNNVISEVQE
                     VWDRIEEEVNKRNKNMEKPYKISLSHGFAQKVKDDGLSLDDLIDKADQKMYKDKQQYY
                     SNKE"
     gene            41250..42095
                     /locus_tag="VKN64_03495"
     CDS             41250..42095
                     /locus_tag="VKN64_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HMA59334.1"
                     /translation="MKNKQYIAFLILIILFSFSLVGQAVDTAVTIVPQKQFVKAVAED
                     KSEVTVMIPPGYSPGNYAPRPSELTKFSEAKIYFSMGVPADVQNILPKVNQFNADIKI
                     IKLANLVGEKYKNRTFPNGSRDPHIWLSPKRVIYMVKIIRDELIKLDSDNEEFYRDNA
                     EEYIEKIRATDQKIRDIIKEKDNNYMLVYHPSFGYFAEEYELKMVSIEEGGKEATAKH
                     LQEIIDFAKENGINKIFYQAEIDSRQTEAIAEEIDAELIKLDPLAEDYLTNLIEMAKK
                     ISGVE"
     gene            42098..42877
                     /locus_tag="VKN64_03500"
     CDS             42098..42877
                     /locus_tag="VKN64_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59335.1"
                     /translation="MEDEKIIEVKDLYLSYNQVNVLENVQLTVYKGQFLAIIGPNGGG
                     KTTLLKIILGLIEPDSGEVKIKGHDIKKGRKLVGYVPQISDFDKNFPINVQDVILMGR
                     ITDQKGLFFNYSEEDKKIAEEIMHDLDIYNIKDRQIGRLSGGQLQRVLIGRALAAEPE
                     ILLLDEPTASLDAEARSNIYSILREINKEITIIVVTHDISAISSHFDSVACLNRNLHY
                     HGDKNITKEDLDQVYGCPVELIAHGVPHRVLADHEGDDHND"
     gene            42870..43685
                     /locus_tag="VKN64_03505"
     CDS             42870..43685
                     /locus_tag="VKN64_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HMA59336.1"
                     /translation="MIEAILQYNFLQNAVYSALMASVVCGIIGTIIIEKNLVMMSGGI
                     AHTSFGGVGLGYYLNIEPIFGALFFALLSSVIIVRIRKRTEVHSDTLIGIMWAMGMSL
                     GIMFISFMQGYPPDMSSYLFGDILTVTDMDINIMLILVIVVVAAITALFNQWKSYLFD
                     EEFSYVRGINTEFYEYFLFILIALTVIVLIRVTGIILIIALLTAPPAMAKLFTYDFKK
                     IIYLAIGFGVIFTMTGLWLSYMLNIPSGATIILLIGVTYFIMASITQRFNVGS"
     gene            complement(43690..44976)
                     /locus_tag="VKN64_03510"
     CDS             complement(43690..44976)
                     /locus_tag="VKN64_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMA59337.1"
                     /translation="MDTEMLSQIYKYEEWFIEHQVSDKELYLIAFFVLAALLIVIFAK
                     RYNIPIVVGYVFLGILLSIEVINAIPFLKLEYKVWYAYIINSFEYVTTLALGFIAFTI
                     GSELSLKLFKKLGKSITYIVLIQGLGAFFMVFIGILLIGYDLYIALLLGAIASATAPA
                     ATVMVIREYNAEGPVTSMIMAVVGLDDALALILFSIVSPIAYNQYSGGASLSINHMIL
                     VPLVEVVGSIIAGILIGYISVKLQEKYEDKTKKVLTLIATLVSGLAAATLLNLSPLIT
                     NMAIGFAYRNFASKNLNIADYVETLTIPLYAMFFILAGTEIRFSGIDIPFIILALVYT
                     ATRVVGKIGGASLGGILSNASENIKKYVGLGLLPQSGVAIALAYTVQQEFTQANIGQL
                     IFNILLFTAALTEIFGSFATKYALVKSDEINKSESA"
     gene            complement(45020..45481)
                     /locus_tag="VKN64_03515"
     CDS             complement(45020..45481)
                     /locus_tag="VKN64_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA59338.1"
                     /translation="MAIVKNYMMRSLNSVSYEDTVEDAIVYMHKTEMPILPVVDEENH
                     FIGTIYSKNILKNIIPEQYGFINSQRILYDRNQAAENLSDIKYRKVEEYMVTKTDTVL
                     ERDNMDKIADIMLNNKESYLFVTNDEGYLRGYISRGDLLYYLLCTEGECDY"
     gene            complement(45484..46434)
                     /locus_tag="VKN64_03520"
     CDS             complement(45484..46434)
                     /locus_tag="VKN64_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMA59339.1"
                     /translation="MITTVTLNPSIDREYFIDEHKPMKDQYIYDNENLKVYPGGKGLI
                     AAINLKNLGYSDVQNIGFIGGKQGLFFEKMVQDFQVTTNYVYTSQEMRNNIKIIAQNP
                     PSNTLYNDYTYSVSERSVKELKKRFKRSIRESDLVMIAGSIPSGVSFDIYNELVEICN
                     ELDKDVYLKASGEALNRALKAEPKFVVPYFKHTHKILDVKVEELDDYIKMGRKLQKEG
                     AQYVIMPFHTNRLLFDNDDTIYMLSPREYCIRNLLGAGDAYNAAFFDFIYQNGFDFIE
                     ANRYAAAAALDIAEHKTVFLHNREEIEKNVDNIIIEEMEV"
     gene            46866..48368
                     /gene="putP"
                     /locus_tag="VKN64_03525"
     CDS             46866..48368
                     /gene="putP"
                     /locus_tag="VKN64_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HMA59340.1"
                     /translation="MDPVLVTFSIYIGGMLLIGYYYYRQNENISDYFLGGRTLNPFVT
                     ALSAQASDMSGWLLMGFPGAIYAGGFNGMWIGIGLAIGTYLNWQFTAQRLRRYTEIVD
                     SITIADYFENRFKDNSRALRIVTALAILIFFTIYVSSGLVAGGLLFENILGVNYQLAV
                     VLAVIIIGLYTFLGGFLAVSWTDFVQGSLMVIALLIVPIALISIRGGLTEVWGQIASI
                     NPDLLHAGKIVSYEFGSEAQWITTSENMSFISLISLLGWGLGYFGMPHILVRFMGIKS
                     AKQIPISQFVGVTWVVVSLIGAVFVGILGIIAAPEALENTETVFLVLLQKLFNPWVAG
                     VFLAAVLAAIMSTIDSQLLVSSSALAEDFYKSLFRPGASQKELLWVSRLAVLFITVLA
                     LFFALSGGTILDIVAYAWAGLGASFGPAILFSLFWKKTSRDGVLGGIITGGVVVIIWK
                     NFFAYTGLYEIIPGFFLSALVIYVISYFGPDPLPEVVDEFERAMKPLINE"
     gene            48388..48696
                     /locus_tag="VKN64_03530"
     CDS             48388..48696
                     /locus_tag="VKN64_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA59341.1"
                     /translation="MRIKEHPILDFDRKEEITFTYNGKEIKAYEGETIAAALYAAGIR
                     VLKTSSKYERPRGLFCAIGNCSSCHMVVDGTPNVKTCITKVEEGMVVEQEVGKGDVSD
                     "
     gene            48689..49813
                     /locus_tag="VKN64_03535"
     CDS             48689..49813
                     /locus_tag="VKN64_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMA59342.1"
                     /translation="MINTDVLVIGGGPAGMTAALKAAQKGASVIIVEKYHELGGQLIK
                     QTHRFFGSKEEQAGIRGIAIADELTEKIESNDKIKVLTEATAQGYYEDKVITVSYQEQ
                     FQKIKPKKVIIATGASEKMLAFENNDLPGVYGAGAVQTLMNLYGILPGEKVVMVGAGN
                     IGLIVSYQLLQAGVKVVAVIEAAPKIGGYLVHASKIRRAKVPIYTSHSIKRAIGEKSV
                     EAVEMVKLDENWNMVEGSEQIIEADTVCLAVGLRPLTDLLIQAECEMAYIPELGGDVP
                     LRDENLKTTNEQIFVAGDAAGIEEATAAMLEGELAGLKAVSEIGYTDPDLFEELNRLK
                     EELKKLRNGPVGKVIRKGLKKARGKEDAGKNRYSHIRGYR"
     gene            49770..50255
                     /locus_tag="VKN64_03540"
     CDS             49770..50255
                     /locus_tag="VKN64_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA59343.1"
                     /translation="MLEKTGIPTSEDIDNIKPDEERLKEGPVAVIECFKEIPCDPCFY
                     SCPFGAIKEFEDINDKPELITEKCTGCGNCIASCPGLAIFVVDYNKKTVSLPYEFLPL
                     PEKGEHVKGLDRGGREVCEAEVVKVRDPKKNDKTAVITVKVPEEYLMEVRNIEVGDLD
                     E"
     gene            50248..50523
                     /locus_tag="VKN64_03545"
     CDS             50248..50523
                     /locus_tag="VKN64_03545"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMA59344.1"
                     /translation="MNDDTIICRCEDITLKELRDLIKEGYTTLDEIKRALRLGMGPCR
                     GDICLELAAREICRLTDKTMEDIKMPNNRPPLKGIKMGELAGDVDEK"
BASE COUNT        16740 a         8095 c        10661 g        15063 t
ORIGIN      
        1 aattgattgt gaaaaaccgt gtaactcctc ttttttgctt tttataggtt cttcatttaa
       61 aatacctgaa ccaccgtatt tttcttgatc agaattaaat acttctttat accttccttc
      121 taaagggacc ccgattttgt aatcttctct cgggaccggg gtaaagttac agaggatgac
      181 cctaaggtcg tccgcttttt taccggaacg ggtaaaactg attacactct gtttatagtc
      241 atcagcttct atccattcaa aaccttcatg ttcataatct tttacataaa gagcattatt
      301 tcttttataa aattcattta agtccttaac atatcttttt atttcctgat gtttgggata
      361 atctaataac agccaatcca actcctgctt ataattccac tcgataaact gaccgaactc
      421 accacccata aacagtaagt ttttaccggg atgggcaaac atgtaagcat aaagcagacg
      481 taagttggca aatttctgcc agtaatctcc ggccatctta tcaagcaatg attttttgcc
      541 gtggactact tcatcgtggg aaagtggtag aatatagttt tcagaatagg catacatcat
      601 ggaaaaggta agcaggttat gtcttccttt tctcatcaag ggatcttctt ccatgtattt
      661 taggacatca ttcatccagc ccatattcca cttgaagtta aaaccaagtc cgcctttatc
      721 aacaggaccg gttaccatcg gccaggctgt cgattcttca gccaccatca tgataccggg
      781 aaagtcctta aagaccacct ggtttaactg tttgatgaag tcgacagctt ccagattttc
      841 attaccaccg tattcattgg cggtccattc cccgtctttt ttattataat ccaaatagag
      901 catattgctg accgcatcta cccttagtcc gtcaatatgg tagacatcaa agaaaaatct
      961 agcactggag attaaaaagc tccagacttc gggctgtccg taatcgaagt ttaaggtatc
     1021 ccactgtttg ttttcagccc ttttgggatc ttcactttca taaagcgggg taccatcaaa
     1081 taatcttaag ccgtgggcat ctttacagaa atgactgggg acccagtcca tgatcacact
     1141 gattccaaaa cggtgggcct tatcgacaaa atacatgaaa tcttcaggtt ttccataacg
     1201 actggtaaga gagaagtagc cggtagtttg ataaccccag gaaccaccaa aaggatattc
     1261 cgtcaggggc atcagttcaa tatgggtata acccatttct tccacatagg gaataagttc
     1321 atcggccagc cgcctgtaat tataggggtc ctcttcttta tcggtcttcc aggaaccggc
     1381 atgtacttca taaatgctaa tcggttcttg atggggcttt ttttcttttc ttttattcat
     1441 ccataggttg tctttccatt catatttatc aagggaagcg acaactgaag cggttttcgg
     1501 actcttttcg gaataaaaag caaccggatc cgatttcaag aaggtatcac catgaaaacc
     1561 tttaatagaa tatttataga tggtgccttc tgtaagaccg ggaataaagg tagtccagat
     1621 cccgctgtct cccacttttt ccatctggtt ggcaaaatta ttccagccat taaaatcacc
     1681 cactactgcg acttgtcggg cagacggggc ccagactgaa aatctgactc ccttttgatt
     1741 attttctatc tttaattgtg cacccataaa tttataaatt tgataatgtt ctccccggtt
     1801 aaagagatat ctatcataat cacttatata tgttttagac attaagtatt cccccaaata
     1861 tatgcttttt taaataattc aagaagtata ttaaatatcc ttttaattat tagaattaaa
     1921 ggtacaaatg gacatctcaa accttgtaaa atatttggtt tgacacatct tagaaagttt
     1981 attaaactta aggtataata acaatatatg agtagaagag gaggaatttt ccaatgtcag
     2041 ataaattaaa gattctattt gtttctcccg aggtatcacc ctttataaaa acgggtggac
     2101 tggctgatgt agccggtgcc ttgccgcgtg aactaaaaaa gaagggacat gatgtaagag
     2161 tagtactacc agagtataaa aagataccgg accgctttgt aatggacttt gaacatgtaa
     2221 ccgattttag aatgaagatg gtctggagag acaaatatgt aggtgttaat aaattagtat
     2281 ataaagatgt accgacttat tttatagaca acaagtacta ttttttccgg gaatccctgt
     2341 atgaaaatgg cgataaagct gaacagtatg ctttcttttc ccgggcggtt ctcgagatga
     2401 tcaataagat cgattttaaa cccgatatta ttcactgtaa tgactggcag accggtcctt
     2461 taagcttgat gctgaaagac aattaccaga tttatgactt ttaccgtgat ataaggaccg
     2521 tattttccat ccacaactta gcttatcagg gcaagtttga ccctgctgtt gtaggagatg
     2581 ttttaggtgt ttctaactac catttaactt ccggtaacat cagacataat gatatggtaa
     2641 actacatgaa agcaggtatc atgtactccg atgttataaa tacggtcagt aaaacctatg
     2701 cagaagaaat taaaaatgag tattatgggg aaggattgga ctatatctta aggatgagag
     2761 acaatgacct ttacgggatc ttaaatggga ttgattatga tgagtttaat ccggctacag
     2821 ataacaggat ctattataat tatgataaaa accacctgga caataagtat aagaacaagg
     2881 ttgccctcca gaaagatatg aatttaaagc cggacaaaga ggtacctgtc atttctatta
     2941 ttaccaggct ggtggaacaa aaaggaatag atttaatccc accgattgta gatgacttga
     3001 tgcaagaaga tctccagttt atcatccttg gtaccggaca accccaatat gaagatttct
     3061 ttagatatat atcttcccgg tatccggata aaattagtgc caatatcaag tatgatgcca
     3121 aattagccca gaaaatctat gcgggtagtg atatgttctt gatgccttcc cgctttgaac
     3181 cctgtggttt aggccaatta ataagcatgc ggtatggtac cttgccgatt gtaaaagaga
     3241 ccggaggtct taaagacacc gtactcccct ataatgaata tgaggaaaca ggctatgggt
     3301 ttaccttcag taattataat gcccatgata tgcttcatac tataaaaaga gccctggcct
     3361 tctataatga tgaggaagtc tgggaaaaac tggtcaaaag agtcatgcag caggacttca
     3421 gctggaagaa atcagcccaa aaatatgaag acctctatag taaattggtt attgataaaa
     3481 aagtaaaaac aaaaccaaaa acaagtggaa aagtagatat caataaagct aatataaaag
     3541 aacttataac attaaatggt attggtaaat cttatgctaa tagaatagta gactatagaa
     3601 ataagaaggg cgaatttaaa gctaaaaaag agttaatgaa tatcaaagga ataggagaga
     3661 gtaaatataa caaaataaag aataaaatac gaatctaaaa agaggtgaga actatgccag
     3721 atctctggac ccatataatt ggtggggata tggtaataaa tgaccttaat gatcgggccc
     3781 tctgtaatgt tatcttagaa aacagggaac actttaacat gggaactcag ggccccgatt
     3841 ttttcttcta taatgatttt tggccctggc taaaaaagaa aaggggtccg aggataggta
     3901 gtttaatcca caccgaaaaa ccgggtgatt ttgtcctggc catttttgag gtgttaagag
     3961 aaaaagaagg ggcaatccag tacccgaaag ccctctccta ttttatgggg tttattaccc
     4021 acatgataat ggatgataaa ttccatagga taataaatga gaggacctat actgatcagg
     4081 aacacaagcg gtttgaacta gaattggact gtgtcttggt taagaactat tataatcaag
     4141 actgtcataa gatcaaccct atttcctata ttaaaattgg ggaggatatg gatgatttaa
     4201 tcaaagacat ctattatcta aacatcaccg aattatacga tgagtctatc aacttcgagg
     4261 taataaataa atcttaccag gatatgctta aggcccaaag aatcttatat agtcctttta
     4321 aagtaaaagc agccttacta aatcttctca ataattttct ttcccttgaa cttaaacagt
     4381 atctttatgc aaatgtaaat agtttttatg tactaaccca aaaagatgta gacgatatag
     4441 aaagagaatt tcctgatttt atcacaacta gtatagattt catgaatgaa gttagttcat
     4501 atctgaaagg tgaaaaaagc agaggtgagt taaaggaact aattgatggt tatttagaac
     4561 aaggcttttt agaaaggaga taaaaatgaa aaataaacta tctattgtag ttgttttatc
     4621 tttgattata tttttaagtc tgacgttagt aagcagtgaa gcccaggccg agaagtttaa
     4681 tatcgcctta agtggaaagg ctcaaacctt agatcccgct tttttacaaa gatctatttc
     4741 agaatggccg atcatgaaca gcatcttttc tggcttggtt aaatacaaac ccggcaccta
     4801 tgaagtggta cctgatctag ctaaggattg ggaattttcc gaagacgata ctaaaattac
     4861 attttattta aaagaaggcg tcaaattcca taaaggatat ggtgaagtga cagcgggaga
     4921 tgtagaattt agtttcgaaa ggattattga tccggataaa ggttcaccgg aagcggaatc
     4981 tttttctaaa ttggaagatg taaaagttat tgataattac acagtccagt taattctatc
     5041 agaaccgatg gcccgtttgt ttacctcaac cttaccctat aatgccggtt taatcgtttc
     5101 caagcaagca gtccaggaga tgggtagaga ggaattctcc agtaacccca tcggtagtgg
     5161 accttatcaa tttgaagaat ggaagataga cagcagtata gtattaaagc gctttgaaga
     5221 ttacttcggt caaccagcct atatagaaga agtagagttt attccgatgg ctgaacagat
     5281 gtcacaggaa ttagccttac gttccggcga gatagacttt ggtgaggtta gtttagaaaa
     5341 ctatcagcag attcaggaaa atgaagatct ggccactgaa acttatcctg accttgcctt
     5401 tcagtgggtg gcatttaatg tgcaaaaagc accgatggat aatccacatc tgagagaggc
     5461 cttaagatat attatagatc cggccgaaat attaattgga gcctttgcag atcaagctga
     5521 cttagcctat tcctggctct tacccgatat gctgggctac aaaaagctgt cccataaaag
     5581 attagatgag gtcggtagag ataagatatt agacctctta gaaaaagccg gttatcccaa
     5641 tggagaaggc ttaaccttaa aatatgttac agatgctaat gacatcagaa aaaatattgc
     5701 tactttagta caggcccaat tagccgaatt taatatccag ctgcaaattg aagccatgga
     5761 aataggtccc aagatcgaag cctggcacaa tggagagtac catataacct atgccagatt
     5821 tggtaatact gttgaccccg gttactgcag ccagtggttt ttaagccgtc aggtcggtaa
     5881 ctggaactta atgcactggt caaatgaaga gtatgatcag ctctgggaac aggcagaaaa
     5941 gaccatgaat gaagacgaga gagccaaact ataccaaaag atgcagacga tagttgatga
     6001 agccgatata gcagtctggg ttacccatgg agtaaaaaca cctacttgga atgatgatta
     6061 tgaagtcact ttaacccctt ctggaggaat cttaagctgg cttgttcaga agaaaagtaa
     6121 ctgataagga gacttaatat gttaatttat acagtcaagc gcctcttatt attaataccg
     6181 acccttttag cggtgatgct tttactattt agcttaagtt atctgctgcc gggtgaaccg
     6241 gctgaaatca tcttaggacc gcgtgcttcc cagcagatga tcaaagattt aaaccagagg
     6301 ctgggtttag ataaaccctg gtatgtcagt ttttacctgt atgtcaagca agtactgtct
     6361 ggagacctcg gtacatccat attcagggat gtaccggtct tacaggccgt tttaggtgta
     6421 ttaccctata cattagcatt aacgctctca ggaatgggta ttgccgttat attgggcatt
     6481 acgattggac tgacggctgc caattaccag aatacaatct gggatggtat cctggaagtg
     6541 ttctctttta tttgtgcctc aacccccacc tatgtggcag gtgtgattat tttattacta
     6601 ttttcaattc gtttagactt tatacctgtt atgggggcgg gagaaggcag tatcttagat
     6661 ttcttacacc acttaatcgg gcccgcttta gccctttcgg tcggctggac cggctatatc
     6721 gcccgtttaa cccgaagttc tatgttagaa actatcaatg aagattatat taccacttcc
     6781 cgttcttttg gtttacccaa aacttttatt tactatcgtt atgctctaaa aaaagcgatt
     6841 aagccaattg tagctgttat tggtcttggt ataggccgcc ttttaggtgg tgccctcttt
     6901 gtagaagtaa ttttcactag accaggtttg ggtagattaa cggtagatgc catctttgaa
     6961 agagatttac cgatggtcag agggggagta ttagtggccg cctttttatt tgttatcgct
     7021 aatctattag cagatctttc ttatgcatat ctggatccga gaataaggaa tgagtaggga
     7081 taaaccatga ataaatatat ttatatcaag aaaaaaataa aatgggtcta tgaaaggatg
     7141 atcggggatt acaggtttta tatagcggga gttattatct tcctcttttt tttaatggct
     7201 atttttggtc cccagattgc cccccatgac ccctataaaa cagatgtgag aagtagattg
     7261 gaagctcctt ccttaaccca cccagccggt actgacagcc tggggcggga taccttaagt
     7321 aggctgatct atgcggctcg ccctgcgttt tatgtggcct tcggcggcct ctttatatcc
     7381 ataattgtag ggatgatctt aggttcaatt gctgcctatt ctggtggtat cctggataat
     7441 ataataatct tcttatttga cgtgatccgg gccttcccgc ccattatctt gatattagtc
     7501 ttagtggcaa tcgtgggacc ctccttacag agtttaatca ttattttagg ggtcaccatc
     7561 atgccgcgtt atggtcgtgt ggtgcgggcg gaaacccttt cagttaagga ggaagagtat
     7621 gtttcggttg ccaagagcat gggtgcctct tcctttaaga ttatttcttt tcatattatc
     7681 cctaacatat tcgcctctgt tctggtctta gccgggatgg atgtcgcttc tatgattatg
     7741 tgggaagccg gtctttcttt ccttggcctg ggagtacaac cacccaagac cagctggggt
     7801 gtcatgctgc agtccggtta tcaatatatt gaaaaagccc ccatgatgct cgtggcaccg
     7861 gccgtggcaa ttatgttaac catgatgggt ttttctcttt taacggaaag tttaagaaat
     7921 gccctaaatc cgaaatctga acagaaggag ggctagagat ggcagagaat ctactagaaa
     7981 ttgagaagct taagatcata tacaaaagaa gggaaaagag atttctagcc ttagactgtg
     8041 tgaatttaga tctaaaagaa ggggaagttt taggagtagt gggggaaagc ggcagtggta
     8101 agtcgacatt gggactttcc atcatgagac ttttaccacc ccaggccagg ataaaaaatg
     8161 gtgaaatctt ctttaaaggc aaaaatattt gtaaaataaa tgaaaaaaag tttaacaggg
     8221 agatgagggg taaagaaatt acgtatattc cccaaaaccc ccagagttca ttaaatccgg
     8281 tctttaaagt gggaagacag ttaaaggata ttattaaata cagggcgcag gaaaactttc
     8341 aaggccaaga tattaacaaa gctgctgttg atatcatgac caagatgggg atcgcagatg
     8401 ccgaaaagag gttagatgaa tatccgcacc aattcagcgg tggaatgaaa cagcgggtcc
     8461 ttttatcaat ggcttttgtt acaaatcccg ctttattaat tgccgatgaa ccgactacag
     8521 ctttagatgt aacggtggaa gcccagatat taggcctttt ggcagattta ataaaagaat
     8581 atcaatcttc cataatatat ataacacatg atttaggagt agtttctgaa atagctgacc
     8641 gagttgcggt tttttatgcc ggtcgggtaa ttgaattaaa taataggtat aatattttta
     8701 acaaccccaa acatccttat acagaagctt taattaattg tttacccggg gataaacagt
     8761 ctgaaaaact gaacgtgatt ccgggtgaaa taccggattt atccgatttg ccgggcggtt
     8821 gtaactttca tccccgctgt aagtatgcaa aagaaaggtg caaaaatgaa aaacccgagt
     8881 tttttgaact ggaaaacggg ggcagaaccg cctgttttta ccacgaggaa gtaggtcgat
     8941 atcaatgaac atcattgaag taagagactt aaaaaaatat ttctataaaa aatcaggtgt
     9001 tttacataaa gtttttgggg tgggccctca ggtaatccag gctgtaagca aggtatcttt
     9061 caatattgaa aaaggtaata cgatggccct ggtaggtgaa tcaggctgcg gtaagaccac
     9121 tgtggcccgt ttaatattga atttgatcga aaaagaagca ggccagattt actttaaagg
     9181 taaagcctta gatgaaatga gtgaagagga atataatgat tatagggaaa atgcggcctt
     9241 tatcgcccag gactttcgtt cttctttaaa cccccgaaaa ctggtgggtg aaatactggc
     9301 tgaacctttt cggattcatc ccggtgtgat cgacaaagag agggaagaag ccaggatcaa
     9361 aaagatggtt gatttggtcg gtttaaatga agaggcttta cttaaatttc cccatgaatt
     9421 ttcgggcggt caggccaggc gaattggagt agcccgggct ttaatgttaa acccggaatt
     9481 tattgtttgt gatgaaccca cctccggtct agacctctcg ataagtgcag ctattatcaa
     9541 tttgatgaaa gaattaaaag aagaatttga tttaacttac ctctggatct cacataactt
     9601 aaatgtggtt cgctatatta gcgactatgt gggggtaatg tatctgggtc ggataattga
     9661 gattgggaaa acaaaagaag tatttaatga tcccagccat ccttattcag aagcactaat
     9721 tgaatcagtg ccttcttttt cgaaagaaaa gaaaaagata aagataaaaa ccttagaagg
     9781 agaagtcccc agcccgattg atccaccgcc cggctgtccg tttcaccccc gctgtaaata
     9841 tagtcaagct aaatgtaagg aggttgtacc aatccttgag aacaaaggaa ataataggga
     9901 agtagcctgt cattttcccc tgtgatatat ttattttatg acaaaaacgt ggtataatta
     9961 taaaaaacat attaaatatt aaactaagga ggatatgagt atgaagagaa gttttattag
    10021 tttattttta ttagtgatac tcctatttgc tatgagtagt ctggtgatgg cccaggatgt
    10081 tgttaagcta ggattttttg cccctgtgac cggaccagct gcagctgatg gtgaaagtgc
    10141 tttaaattca gccaaattag ctgttgaagt gatcaatgaa gagggcggta ttgacggtag
    10201 agaagtccag ttagtagagt atgatgacca ccttgattcc aaacagtctg taagtattgc
    10261 ccagaaactt accaccagag acaatgtggt agcagtagta agtggttctt atagtggaac
    10321 tacccgtccc gctgctacaa tctatcaaaa tgcgaaagtt cctatgatat ctgcctatgc
    10381 tattcaccct gatatcccca aaacaggcga ctatatcttc caacaatctt tcccaggacc
    10441 cgtacaaggt agagttggcg gctatatggc agttgatgac ctaggagctc aaaagattgc
    10501 tatcctttat gtagataatg atttcggaag taccttaaat gataatttcc aagaatatgt
    10561 taaagaaaaa ggtgccgagg tagtttatgt tgactccttt gctattggtg aaagggaatt
    10621 caaccccgtc ctgaccaaga tcaaaggttt aaatcccgat ttaatttatg tggtagccta
    10681 tgcaggtgaa ggtgcagcaa tcatgagaca gtctgttgat gtagggattg aagcacagat
    10741 ccttggtact gaaggtatgg attcaactac ccagttctta caggtagccg gtgaagcggc
    10801 ggaaggatta attattacaa ctaaccttaa ccgtgacagt gaagaagaac aggttattga
    10861 ctatattaac cgttacactg aaaaatatgg ttttgcacct gacatggtgg gagcctctac
    10921 ttatgatgct ttcatgctgt tagctcacgt aatgagacag gttggtcccg attctgaagc
    10981 aattaaagat gccatgtatg aagtagaaga ctatatgacc aacaccggtt tattatatag
    11041 atacactgaa gtgggagaag cgattaaacc ggttcaggtc caggtcgtaa aagacggcga
    11101 attccattac tatgaccaga ttactgacat ggacataatt gctccgtagt ttttacaaaa
    11161 aattgatata agagcataag atattcaagg taggaaggct cttgcagcct tcctactata
    11221 cttaattaaa aggagagtat agagatgttt ttacagcagt tgttaaacag tattgcaata
    11281 ggtagtgttt atgctcttac ttccatgggg gcgactttaa tatacggtat tttaggaata
    11341 ttggatattg ccaatgcggg agcttatgtc ttaggagcct atctgggctt attattctac
    11401 tcgctaacag gtaatattat catttcattt gcggcagcaa tggctacaac cggtctctta
    11461 ggtattttaa tacaaaaata tgtatataga ccgcttttaa gtcagcccgc ctatatccct
    11521 ttaattgcca gtatcggtat ctttatcttt attgagaatg tgattaggct gattgcaggt
    11581 ccgactatca agcagtttac ggttaaacta cccttcagta atttagatta tgccaattta
    11641 catattaata atgtctgggt actgatcttt attgtggcct tgattttaat gtttttactc
    11701 tggtatttat taaaaatgac caaaataggt ttggcctggc gagctacttc ccaggatcta
    11761 gatatagcca aaacaatggg gattaatacc aataaggtga tctcgcttaa tttctttttg
    11821 ggttacagtt ttgccgcctc ggccgggatc ttgatcggta ttttatataa ttcaatctac
    11881 cctactatgg gtagtgttgt ttcttataag atgctggcta tcatagtact gggtggttta
    11941 ggcaacccct ttggtactgt catagcggcc ttactgatcg gatttattga aacaatggtc
    12001 ggagcctata ttggattttt cataccccgt aatgctatag cctttgtcgc attagttatt
    12061 atcttgttga taagtcccaa gggcttattc acacagaaat ccaggatata ggggaggaga
    12121 gtatatagta tgtatgcaat cgtagttatg atcaatgtcg gaatatttgt cctgttagcc
    12181 ttaagtttaa atataataac tggttatgcc ggccagccca atatcgggca gggggccttt
    12241 tttggaatcg gagcttatac gggggcggtt ttgaccggta agttcggaat atatttctgg
    12301 ttagcaatgc cttttgccgc tattataact ggatttatag gttttttctt gggattaata
    12361 agtataagaa taaaagaaga ctttttagct attgtcacga tagccttgaa cttcatagtg
    12421 gcagctgtct ttcaaaatgt ggagttcttc ggggcggcca tgggcttatc tgtccctccc
    12481 cctaactggt ttggcgaacc tttatcttac cagggttatc ttacaatagt cataatttta
    12541 attatcctgg tagtcatttt ttctaagtgg atcgaaaaat cctggctggg tatggcctta
    12601 ggtgcaatta gaaataatga gatggcggct gattcattgg gaattgacac taaaaagttc
    12661 aagataatgg cttttgtgat tgggacctca attgccggtt taaccggggt cgtatatgcc
    12721 cattacatga cctttattat ggcctctgat ttcggatttt taacctcggt tactatttta
    12781 tcgatggtaa ttttaggggg gaacaataca attaggggtc cgataattgg agcagtcatc
    12841 ctgggtttaa ttccggaagt gttccgcttc atatctgatt acaggttatt agtctatgcc
    12901 ggtattttga tcttgatgat caggtttcag ccctccggac tgcttggtga tgatagcttt
    12961 gtctggaata aattaaaact attaaaggca tccttaggtc gaaggagggg tgcttaagat
    13021 ggaaaatatt ttaaagatta aaaatgtaga aaagcacttc ggtggattga ttgccctcca
    13081 tgatgtttca tttgatgtta agaaaaatga aataaccggt ttgattggac cgaacggggc
    13141 cggtaaaacc accttattta ataccctttc aggcgtctat aaggcaaatg gaggtaatat
    13201 tttctttaag gaatcagata taattaacct gcctccctat aaaatagcag atatagggat
    13261 cgctcggacc ttccagattg cccgtccctt tttagatcta accgtatccc aaaatgtcct
    13321 ggtcggcttg ggagaaaaaa attaccaggg ctttttaaaa ccatttaagc tgagtatcaa
    13381 tgcggtaaat attaaaaagg cccatgatat attagaaatg gtgggaatga aagagtttag
    13441 aaaaaatgag gcggctgatt tgagcctcgg ttacaaaaga aggttaggca ttgcccgagc
    13501 tttagcttta gagccggaag ttctaatgtt agatgaaccc ctggccggtt tgggtaaaga
    13561 tgtttccgag aaaatgttag acctggtaga agaccttaga aaagaaggga tgaccataat
    13621 aattattgaa catgatatgg aagctatcat gagtatctgt gataagattg tagttttaaa
    13681 ccagggagaa aagatagctg agggtaagcc aaaagagatc caaaataacc acgacgttat
    13741 agaagcctat ctgggaaagg atgagttaaa tgcttgaggt taaaaatatt aatgtatttt
    13801 acggtgaatc tcaagcccta tatgatgttt cattaaaggt cgaagcaggt gaatttgtaa
    13861 ccataatcgg ggcaaatgga gcgggcaaga ctacaataat gaatacgata atggggttga
    13921 tcaaacctaa aagcggtcag attatttata aagataaaga tttgaccaag ataaacccct
    13981 gggaaagaac tgatttagga atttcttatg tgccagaagg gcgaagaatt ttccccgatt
    14041 taacggtcat ggaaaattta agagtaggtg cttaccgctt aaaagatgat agtcagctgg
    14101 cttctaactt tgattatgtc tttgatcttt tcccacgtct ggccgaccgg aaaaatcagt
    14161 tagccgaaac gatgagtgga ggcgaacaac agatgctggc tattgccagg gctttgatgc
    14221 tttctcccga attgatatta atcgatgaag tttcgatggg actaatgcct attttagtta
    14281 ataaggtttt tgatgtaata gaaaggttaa atgaacaggg tataactgtt ttgttggtag
    14341 aacagaatgc gcgtaaggct ttaaactacg cagatcgtgg ttatctttta gaggttggta
    14401 atattgttaa gtctgacacc tgtgagaatt taaagtgtga cgagatgatc aaaaaggctt
    14461 atttaggagg ataattatgt gcggcagata ttttttaaat attgccttag atgaattaga
    14521 aaatagatat ggaataacag atatcccggt cgaaataaaa gaacatcagg gagaaatatt
    14581 tccttctaat gaagcaccgg tcgtgtttaa tgaaaacaaa caaaaaagtg tgggtttgat
    14641 gaattggggt tttagaccct cttattcgaa taacttggtg attaatgccc gcagcgaaac
    14701 tgttcacaaa aaaaatctat ttaaagaatc ttttcatagg aggagatgta taattccggc
    14761 cagtggtttt tatgaatggg aaaaagtagc agataaaaag ataaagcact ttattaatat
    14821 tcaggaagat gaaataatat cattagccgg tatctatgat ttctttaata ttaacggtaa
    14881 acagcagatg gccttctgta ttttgaccaa agacgcccca caggaactta taaatataca
    14941 tgaccgtata ccggtaatca tcaaaaaaga atatgaaaat gaatggttag aaaataaatt
    15001 tacgcccggg atattcgaat atttacagcc aaaaggtttt aattttcagg tggaagcctt
    15061 gtaataagtt tcacacgagt ctgaaaattt taataattag caggatttct tgtatacaat
    15121 attgaatata tatttacaag agttcaattt aatattataa aaaagagaag aaataaaagg
    15181 aggagagatt aaattgaaaa aactcttatt aactgtttta accttaactc taatattttc
    15241 attaagtgct gttggtttgg ctcagactta tactgtaggg actagtgccg gatttccacc
    15301 tttcgagtat gtagaagatg gagaaattgt tggttttgac attgatttaa tgaaggcaat
    15361 tgctgaagag atggactttg aagtagagtt tagagatata gcttttgatt cattaccttc
    15421 agctgtaaaa actggtaata ttgatgtaat tgccgccggt atgacaatta cagaagaaag
    15481 agaaaaggct ttagatttta ctgaatctta ttattctgca aatcaaagta taattgttca
    15541 agaagattca gagatgaata taagtgttct atatggtgat catgatataa gtgttcaaac
    15601 aggtacaacc ggtgacctct gggtaaccga aaatctaaaa gatcaaggta ttttaaccgg
    15661 agatgttcaa agatatgata cctttgtact atgtgtaagt gacctcgtaa acggtaatgt
    15721 tgatgcagtt gtattagatg aacccgttgc ggaaaggtat gctgaaatga gacctgttaa
    15781 gatagtagga gttattgtaa caggtgaaaa ttatggctta gcagttaatg aaggcaatac
    15841 cgaattactt gaaactctca atgaagggtt aagaagagta agagaatctg gtaagatgga
    15901 tgaattagta gcaaaatatt tccaataata tttgactaaa atattataga gaggatgtat
    15961 gacttatcgg aaacttagag cttatcatga gttcccttcc aaatcttttg gaaggggctc
    16021 ttgttacaat acaattgaca ttacttagtt tattaatagg acttgtacta gctattccta
    16081 tcgcatttgg tcaaatctac ggccccaagt ggcttcgtaa actgattgga gtttatgaaa
    16141 gggtagtcag aagtatacca cttctcgtaa tcttattttt aatattttat ggttttccta
    16201 gaatagggat aaaatttccc gcttttactg ccgctgtttt agggataggt atcagaagtt
    16261 cagcctatca gtctcaaatc tttagagggg ccattcagtc tatcagtggt tctcaaatga
    16321 aggcggcgct ttcgcttggg atgacaaaga ttcagagctt taaaagtatt atcttacctc
    16381 aatcaatcag aattgcactt cctccgatgg caaatgaatc tgcaattgtg ctaaaagata
    16441 catctttagc atatgcccta ggagttattg aattacttag acagggtgga tatataatag
    16501 ctactgaata tgcgccactt ccgatatatt taactgttgc ggcaatctat ttagtgatga
    16561 ccttaacaat taataccacc ttgggtcatt ttgaaaacag actcaagatt ccaggtatag
    16621 gaatagagga gagggtaaga tgagtgaaga aaaaagaact atacttaaaa tcgaagatct
    16681 tcataaaagt ttcggcaaca atgaagtact aaaaggtgtt tcactggaat taaaacaagg
    16741 agaaaacaag gttattattg gtcccagtgg aaccggtaaa agtactcttt taaataatgt
    16801 taataggctt gttccacctg atcaaggcaa aatttatctg gatggggagt tgataacccc
    16861 tgagaatata aataggatga gacaggaaat aggttttgta tttcaggatt ttggtctatt
    16921 taatcattta actgctcttg gtaatgtcag ggtaggatta attaaggtta agaaaatgaa
    16981 taaaaaagat gcgactgaac ttgccatgga agagttacgt agagttggtt tagaagaaaa
    17041 tgctgatcaa tatccggctg agctttctgg gggtcaaaag cagagggttg gcatcgctcg
    17101 tgcacttgct atgcagccta agttaatttt atttgatgaa cctacatctg ctttagatcc
    17161 tgagctgatt ggagaagttt taaatgttat gaaggacttg gctcaggaag gtatgactat
    17221 gttggttgtt actcacgaga tgggctttgc ccggaccgta tcagatgaga taatttttat
    17281 ggaacacggt aatattgttg aacagggtcc accaaaacaa ttatttaata atccagaagt
    17341 tgaaaggaca agagaatttc tatttaagtt gactgagtta tatggtgagg gtgatgagta
    17401 gtgagtatgt atgaagtatt tgtaggagcc tggcctcatc tatttgaggg tgtgcagtta
    17461 accctcttat taactgtaat atcaattata accggtacga taatagctat atttttatct
    17521 ctaggtaaaa catatggacc taagtatgta aggtggccaa ttaatgtttt tatagaatta
    17581 attcgtggta ctcccttatt agcccagttg tttatcctat acttcggctt accgccatac
    17641 gggattaaat tatcaggctt tttagttgca tatattggat ttactatgaa ctgttctgcc
    17701 tatacagcag agtatataag gggatctatt atgtccattg agtctaacca gttaagggtt
    17761 gcagaatccc tcggtatgac aaaatggcag ggtattttta atgttatttt accccaggct
    17821 ttaaggagag ttgttccatc ttggaccaat gaatttatct atttattaaa gtatacttct
    17881 ttggcctata tgatcggtac tcacgaattg atgactcaag ccaagttttt tgccagccgg
    17941 aattatgaat ttttcaaggt gtacttaata gttgccttgt tttatctggt aattgttgta
    18001 tttttcaccc aaattttctc ttgggtagaa agcaagttta caattccagg ctttgaagta
    18061 gaacattaaa agattaaaaa ataaatttac aggtctttta atgaatgatt agctaaattt
    18121 tttactccct tttttataaa aaagggagtt tttgtagttc ttaatatatt tttgatttta
    18181 attataatat attagtagtt gtaattactg cccttaatat attataatta aagttgatat
    18241 ttaataaaaa tttttaaaaa ggttttcatg atgaggtgtg aagatgacaa agaatattga
    18301 aattatctat gaactattta acttattaga gaataatgaa tataagaaaa tactaaagag
    18361 aatatgtgag cacttagatg ttgagcgtgc ttttttactt aactactatg aaaactataa
    18421 tacaactgac atggtaatag gttggaatat gtcagatgaa attcatgact atctcaaaaa
    18481 tacagatata gaggagttta cccagaccaa aaaacagtta gcccagggta aaccaatata
    18541 catttccaac ataagtcagg aaaataatta tcccagattg gtagaattat tgagaagtta
    18601 taatcaaaat tcggccgttt tagttccgat cagggatgcc gattttgagc tgattggtat
    18661 tatggggatt gcatctgaga atacgctgct ttgtgaggaa aaagaagact gtcaaaagat
    18721 tttagagatc ctcacctata atctaaaata taattgggaa gacgagatat taaaattaaa
    18781 gtatcgatct ttaattcaaa attcagcttc tgcaattgtg attttaaatg atgagggtaa
    18841 catagttgaa ttaaataaaa aatttgaaaa acttgccaat agtcccaaga ctgaattaaa
    18901 gaatcaaaaa tgtttttttg attttattgc cccccgggat aaagaggata tgattaaacg
    18961 gcataatgaa agaagaaagg gtaaagaggt tgtttccgat tatgatttta actttataga
    19021 tagtgataat agaaaaaaag aggttaatgt taaagttaaa atgatacccg gaactgataa
    19081 aagtgtctgt tccttggtcg atatcactca aaagaagcgg accgaaaact tattaaaact
    19141 gcaagaagag aagctgtctg aaagctataa tcaattaaaa caaagtaggg accaattgga
    19201 aagcacttat ttagatttaa tcgataaaaa tattgaaaat gagaggctct taaacaatat
    19261 tgagaaatta atagacctaa tgagttctct tcatatgtca aatggaaaca acttgaacaa
    19321 ctttctagct gaactgttat atacggcttt agaaatagtt gatgtggcag attacgggag
    19381 tgtctatatc tttaatgaaa atgactgtat agaatacatt gaaacagtcg gccataattt
    19441 aaagaagtta aaccagattg aaattgaagg agaaccgttt aggcataata ataaagaggt
    19501 tgagatagtt gataatatcc tcgataaaaa caaagagtat atgagtgatt atataattca
    19561 aaagataaag gatgcttctt taccaatcaa acagtctata cccgttaccc tccaggaagg
    19621 taataatatt tttggcggga tctgtataga tattgcacgc ggaagcaaaa aagagttttc
    19681 cagggatgat aaacgtttaa taacatcttt taaaaatctg gccaatacct tttttaagtt
    19741 agaaagatat aataggttaa acagccagtt taataaggag ttagtaggtt ctttactgaa
    19801 ctttttaaac cagtatgatc tttataccaa taatcattcg gaaaatgttg cccaactcag
    19861 tttagaaatt gcagaaaaag taggactaag taaggaagag aaaaataata cttactggac
    19921 gggtatgctg catgatatgg gtaagttaat cattccagcc cgtatcttaa ataaagaagg
    19981 tttgttaacc aaagaagaat atgctgtcat aaaagaacat ccaactttat gttacaatgc
    20041 tttaaaagat tctgattttc ttaaagatat tgcccagtat gttttacacc accatgaaag
    20101 ctgggacggg agcggttatc ctgccggttt gcagggagaa gagatccctt taatatcaag
    20161 aataatagca gcagccgatg cctgggatgc catgcgttcc gaacgggctt accgtagttc
    20221 tttaagtaaa gaagaagcta aaaaggagat taagattaac cagggtatac agtttgatcc
    20281 ggacattgca gaattattat tagaaatagt ggagtagccg agcagaaagg aatgcttaaa
    20341 gatgaacaat atcctattaa atatcttatt agtaattttt ttagtatatc tatttaataa
    20401 gttaaaaact aaatatagtg attcacttga tatgccaaac tatcttagct atgaaaattt
    20461 attcgataaa ttagaagtac ccatgctctt attgggaaaa gaaaatgaag ttgttagata
    20521 taatgactct tttattgatc agtttgattt agatgagaag gagatagaag gggaaccatt
    20581 taattctgtt tttgaaatag aaggttcaga aactgggttt aaccatatat tagaaaagtt
    20641 taattcgggt gagaagtata aaggtaaaat aatgtttttt gataaaaagg ataagagata
    20701 tgagtgcgaa ctcaaattat atcccatatt tgagaacgat gaattggtag gtaaacatat
    20761 cattattaat cagatcgata aagaagaaga gaaaatatct tctgctttaa gagaaagccg
    20821 ccgtaagata agggacttac accagaccgc tttaaagatg gagaatgtaa gaactaaaaa
    20881 ggaagtatat gatttaaccg taaaagctgc ggaaaatata ttggattttg atctctgcac
    20941 cctagatata gtggaaggta ataagatggt tgtcaaggcc acatccagta atattatccc
    21001 gggtgaatca atctccagtc ctttagatgt tgactccata gcgacccagg tctacaaaaa
    21061 caaacaggcc ctgttatcac cggatatcca gaaggaagag tttgctaatc ccaccagttc
    21121 caaataccac tctgccttaa ctattccgat cggggaacga ggcatatttc aggcggtttc
    21181 gaccaaggta aatgagttta cgagagaaga tttagaatta gtggaacttc tcatcgctca
    21241 cgccctttcc gctttacaac ggttagaaat ggaccaagaa ataaggtact tgggtttcca
    21301 cgacagccta accggcttat acaaccggca ttatcttaat gaggaaatta atagattaga
    21361 tacaaaaaga cagctcccga tcagtgtaat aatcggagat ttaaacggtc taaaactgat
    21421 caatgatatt ttcggacatg aggaagggga taaattttta aaaaaagcag ctgaattact
    21481 gaaagactgt gtaagagagg aagatatact gggccgctgg ggtggagatg aatttatgat
    21541 tattctgcct aaaaccagct gccaggaggc ggatgagatc ctggccagga taaatcggaa
    21601 agtccaaaaa acatataagg aaaagagacc gatttcaatt tctttagggc gtgctacaaa
    21661 ggatatcaac tttgcagatt cctttaataa agtaattgaa gaggccgact ataagatgta
    21721 taaaaataaa gctattatgc atgaagaaga taataatccc ttagtggaaa tgattaaaga
    21781 aaagatgcag aaaaaggaac taaaaaaagg ggaagtctat agattggaca gccttttaaa
    21841 catgtttaaa aataatccct ttgagagata attttataaa atacgcagaa attcattaag
    21901 ccgggtaaaa ccggcttttt tgaattcatt gcacttttat tcactttgta atataattaa
    21961 atttagcata taaaggaggt aacatgacaa tgcaggaatt attaaatata aaagagctga
    22021 acttaaccct tgataaagaa agaatcttaa gaaatataaa tttaagtttg gaacgagggg
    22081 aagtatttgc agtaattgga ccaaatggat gtggaaagtc ttcacttgca tatactatta
    22141 tgggtctaaa aggatataca caaaaatcag gtgagatcaa ctttaaaggt gaagaaatta
    22201 gtgaactttc tgtaactgaa agggctcgca aaggtattac tttagcctgg caggaaccgg
    22261 cccgttttga aggggtttcc gttagagagt ttctatcgat tggggcccgt tctagaggcc
    22321 gcgagatcag tgaagaagat ctaaactggg ctttaaatga agtagcaatt gtcccgaaaa
    22381 aatatctgga tcgtaacgtt gatgatactt taagtggggg cgaaagaaag agaatagagt
    22441 tagcttccat acttttaatg aagcccgatt tagttatctt agatgaacca gattccggag
    22501 ttgatgtggt agcattaaat aacataggtg atgtaatcaa ggactttaaa gacgaaaata
    22561 tcggtgtttt attaattaca cactccgatg agatgctgga agtagctgat aaggctgctt
    22621 taatctgtgg gggagaagta tttaatgtag gtgatccaca ggaagtaagc gattacttta
    22681 aatatgaatg tatgccctgt gaagatgaga accccgaaga gcatcaggag gtgtatttcc
    22741 gtgaatgatt atgaaatgat ggtagatgcc tataaagagg cgggcggaga agacgtattt
    22801 tcagacagcg ataccgctca catggtatta gaacaggata aggtcttagg caaacacgaa
    22861 gtagaaggtc tcataatgga ggctgaaaaa atagatgata acactgtaga tgtaaagttg
    22921 agagtaaaag aaggatataa gataaaaaat cccgtccata tgtgttttgg agtccttccc
    22981 cgggaaggat tacaaaagat aattatggat gtggagatag aaaaagatgc cgaaatcaag
    23041 gtaatgtcag actgtgtttt tcccaatgct actaaggtga aacatgtgat ggaagcagat
    23101 atccatttag cagaaaattc gaagtacgaa tatgaagaaa accactttca tggagatacc
    23161 ggtggggttg aagtaattgc caaggccgat attataatgg atgaaggcag tgaattattt
    23221 accctattta ccttaagaaa aggtagggta gggaagatcg attttgacta tgaatcagaa
    23281 ctggccgcta atacgaaagt cgaaatgtta actagaatca gcggttatga agatgataag
    23341 gttaaaatta gggaagcggc ccatttgaat gggtacaatg cccgaggtct tttagattct
    23401 aaaatagcga tggtagatga ggcacaggca gaagtatata atgaactgac tgccagtgct
    23461 ccggaagcta aaggccatgt agactgtaca gaaataatta aaaataatgc ggtggcgcgc
    23521 gctatcccca ttgtagaagt taaacatccg gaagccaagg ttacccatga agcagctatc
    23581 ggtagtgtag acagtgacca actgcagacc ttgatggcca ggggtttaga tgaagaagag
    23641 gcttctgatt tgattattca ggggctgtta aaaggataaa aataaacggg gtgaaataaa
    23701 tgagaagaag acctggtata actttaatta taacgtttct atttttactg ctgtttagtt
    23761 tttctttgac agtaggggcg caagattttt atacaatttg taaggaaggg accccggctg
    23821 aaattcagca ggcgatcgat aatggcgctg aagttaacca acagaataaa gaaggaatta
    23881 caccaatgat gacagctgcc agatataaca gtaattataa ggcggttgtt accttatata
    23941 ataataacgc ttacgttaat gcaagagata atgaaggctg gaatgccttg atgtatgctg
    24001 tcagatataa tgataatatt gatgtaacga gaatattact taagatgaac tcaagggtta
    24061 atatagatga tacagagggt actacccctt taatattggc agtaaagtat agaaatgaaa
    24121 aaatggttag attattacta aattatggag ctaatgcaaa taaagcaaat aatagaggcg
    24181 aaactcctct tatgcttgca atacaagatg accttaacgt tgaaatcatc gatgccctct
    24241 tactatcagg ggccaatgtc aatattagag ataaacaggg gcgtactccc ctctactatg
    24301 ttattgataa gggagattat ggtttagtaa gaaggtttat tctctttgga gctaaagtta
    24361 aaattagtga ttatgatgac tttaccccat tgatgatggc tgccaaggaa gcaaaaaatg
    24421 taaagaccct taagatatta aacatagaag atagtaatat caatgcccag gatgaaaatg
    24481 gaatgacggc tttaatgtat gcagcccgtt ataataataa ccaatctgtc atgaactact
    24541 taattgatga gggagcccgt attaatgcga ccgataataa gggcaataat gctctgactt
    24601 atgctgtaag atataacagc tataatatta tcactctgtt gttaaatagg ggagcttcct
    24661 tacaacagga aaatgtagcc ggtaagacac ccctcttttt agctgtggaa cggaaaagag
    24721 atctacagat cttatctctt ttggttagtc gtggtgctaa tgtaaaagat agagataaat
    24781 tcaagggtca gactattttg atggcggccg tagtaaatta tgataacccg gccattatta
    24841 actacttaat tgaccagggg gcaaatccga ccgcccgaga ttttaatgac aaacgcgtag
    24901 ttgattatct ggaagagaat aatgatttaa aaggaacgga tctctactgg agattgaatt
    24961 acttggaacc tgataaaagg cgtaagtcac ttttagattt taaggataaa acaagtgtag
    25021 catttacctc agctgtgata ccttcagccg gccattatat ggttgacagc tggtggccga
    25081 aaggtaccct ctttatggtg ggggaagtcg gttctctaat aaccgctctt gcctctgatg
    25141 atgagggaac ccggggaact gctctaacag tatttggagt tttaaaagta ttagaaatag
    25201 ttgatgtctt aaatgaaacc gaaaaatata atgaagatgt agaagaatac aataaaaagg
    25261 caagggagtt taatgaacaa ctggagaatt aaatttttta aaataaatta tcttacacaa
    25321 ggagtgagag ataatgaaat tagcaaaagg aaatataact gttgtcatgt tgtcacttgt
    25381 cttaatttta accttattat ttgggggagt ggttaatgcc gcccctaaag aagtcgtaat
    25441 tcagacccca cctgtaccgg cagctctgcc tttacttaga ctgcagaaga caggtgaact
    25501 ggataacttc ttaaaatttg atgttaaagt ttcccctgac catcagagag caatttcctt
    25561 aatttcaaaa aacgaaattg actttatggt gaccggggtt aatgtcggtg caaagatata
    25621 taacaagggt atcgatgtca agatggtcaa cgttaatacc tgggctatcg attacctttt
    25681 aacaaatggt tttaaagctg aaagctggtc tgatttaaaa ggtaagaccc taagtctacc
    25741 tttaaaaggt ggaccgctgg actttttagc caggtactta ttaaaagaaa acggacttga
    25801 tccccaaaat gatgttaatt tagtctatag acctctaccg aatggggcta agtactttat
    25861 gtccggccag gtagactcaa ttattctgcc cgagccgctt gtctcagtaa gtttaactaa
    25921 aagcgataag gcacaacttt ccctggatat ccaaaaagaa tggggtaagt tacacaatgg
    25981 tgatgaaagg attccctttg taggattatt tgtctccaat caattcgttc aggaatatcc
    26041 tggattaacc gatataattg caggtaagta tattgagggt gtcaagtatt ataacaataa
    26101 tccggaagaa gctattaaag acgcttctaa ttactttaat gtaccggaac ctgtgatgaa
    26161 aaaggcctgg agtagagttg atctcagtct atatcctgat agtgaaagca gagatttagt
    26221 caatacttat tttaaccaca tactggagat gtatcctgaa atgataggtg gtaatttacc
    26281 agatgaagcg ttttattatt aataaattta aaaaaatatt attgagcacc accggatttt
    26341 tggtggtgct tttaatctgg atttattatt ccagacagat gaacccctta atattacctt
    26401 ctcccctgca gacatttaat gcttttatag atatctatca aagcgggaat ttatttaata
    26461 acttatttat tacgattcgg agaacaataa ttgggtatgg agcagcttta ataattggtt
    26521 ttatgatagc tttaatctta aataaatcta ataccttata tacgataaca agaccgatta
    26581 ttactgttat acaaaccact ccacctgtta tctggctagc gttagcagtt atctggtttg
    26641 ggatagcgga taatcttaca cctatttttt taatttttat agtaactctt cccatcatct
    26701 ttgttaatat cttcgaaggg ttacaggata tagatcaaaa cttaatagaa atggccgaag
    26761 tttataaggc agaccggcaa gagatatttt ttgacatcta tgtaccttcc cttatccctt
    26821 acatattttc agctttaagt atcgggatgg cctttgcctg gaaatcaact atctttgccg
    26881 agtttttagg ttctaatagt ggagttggtt ttgccttaag catggcaaat agtaatttaa
    26941 atactgataa actttttgcc tggggcataa ttttaatcct attaatgtta gctgttgaat
    27001 atctgataat aaagccggtt caaaagaaag ctacaaggtg gaaataaaat ggagaaaatt
    27061 ctagaattca aagatattta taaaaatttt gctgaactaa aggttttaaa agatgtgacc
    27121 ctccatgtca ataaagggga gatagttacc ctattaggcc cctcaggaag cggtaaaacc
    27181 accctattta aaattgcggc cggcctgtta aaacaggatc agggtcaggt aatgagggca
    27241 gacgaactaa gaaccggtta tgtgtttcag gaaccccggc ttttgccctg gcggaatatc
    27301 aaagataact tccatttcgt acagcagaac tacctgccga agaagaaagc ggccagtata
    27361 aggaatagcc tattaaagtt aaccaatcta gaacaaaccc tagataacta tccggctgaa
    27421 ctttccgggg gtatgaaaca aagaatcgag attttaaaag caatttccat aaaacctgat
    27481 tttttaataa tggatgagcc tttaaaatca gtcgataccc agacagaagt taatttaaga
    27541 aacttgcttt taagactcca tatagaaaga aatatcgata tgtttatcat aacccatgac
    27601 ccggaagaag cagccctaat ttcagaccgg atctatgttt tatcggataa accgggtgtg
    27661 ataaaaaaag agtttaaaat tgagaccaaa aaagaagaca ggactttaaa acatgatgag
    27721 atatatagta ctatcgatga gataataaat atttttatgg atttagtaga ggattatcag
    27781 tggaacagag accatgtgga ttctatcgaa taatttttac ttttttaatt aaataagata
    27841 aaatagaaaa aaatattaat tttttaaggg ggcaaaatta tataatgacg gagaaaaaaa
    27901 tcacaataat aggagctgga accggcggtt atgtggcagc cataaaagca gcccaattag
    27961 gcgcagatgt aactttaata gaaaaagaaa atatcggtgg tacctgtctg aattggggct
    28021 gtataccgac taaggcctta gtatattctg cggaaaagta ttctgaaatg caggaagcca
    28081 ggcgatttgg tattgaagta aatgatgtta atttaaatat gaaaagggta atcagaaata
    28141 aggacacaat cgttaaacaa ttggtaggag gagtagaatt tctgctagat aaacacaatg
    28201 taaaggtgta taaaggcgta gcaagcttac tcgatcaaaa tacagtttcg gttaaaacag
    28261 aagatggtag agagattatt gagacagatt atataatttt agctaccgga tcaaaggtta
    28321 aaaaagcccc tatcaaaggt atagatatgg aaggtatttt aaccagtcga gatatcttag
    28381 atttagaaga actacctgaa tctttagcgg taattggtgg gggagtaatc ggaatggaat
    28441 ttgcctttat ctatgctaac tttggggtag atataaaggt tgtcgaatat ttagatgata
    28501 tcttaccggg agttgatcag gaagttactt ccgaacttgg taaagttgcc aaaagagcta
    28561 aaatagatat cacaaccgga gcccaggtca aagaagtggc taaaacagac agcggctatc
    28621 aggttaaata cttaaaagac gaagaaaacc aaactataga agttgacaaa gtactggttg
    28681 caaccgggcg tgaacctagt tttggcggct tagatttaga taaaataggt gttgaaaaag
    28741 aaaataatgc gattaaagtt gatgagcatt taaagactag tgttgataac atctatgcag
    28801 tgggagatgt taccggtaag atccttttag cccatgttgc ttcccatcag ggggtagttg
    28861 cagtagaaaa tattatgggt gaagagaaaa agatggatta tgatgcagtt ccagctgcaa
    28921 tatttacaaa gcccgaaatt gcgactgtcg gtttaacaga agaagaagtg aaagaggcag
    28981 gtattgatta taatataggc aagtttccat ttagggccaa cggtaaggtt aagaccatga
    29041 ataagagaaa tggttttatt aaaataattt ctaaaaaaga cagtggggaa attttaggta
    29101 gttcaataat cggtgtacat gctaccgatt taatacatga actaaccctt gccgtcaata
    29161 aaaatatgac aatcgaggat attatagaaa ccatacatgc ccatcccaca acctcggaag
    29221 taattcatga agcagccctt gattcgatag gaggagcatt acattatgtc agacttaata
    29281 gataaattca gagaaaagat cgaacatgcg aggatagtac attccgattc gtttaatccc
    29341 tggtataatc tagctttaga agaatatttg ttaaataata ctaaaaaaaa tgaagtgata
    29401 ttatacctat ggcagaatga taacaccgtt gtaattggca ggaatcagaa tgcctggaaa
    29461 gaatgtaact gccgcttatt agaagataat gatggtaaat tagcccgccg gctttccggg
    29521 ggaggggcag tctaccatga tttaggcaat ttaaatttta cctttgttat ggataaaaac
    29581 ctttatgata ttcataccca acttgaagta atattgaaag ctgtccggga tagtaatatt
    29641 gaggcagaat tttcgggtag gaatgatttg gtagcatccg gccggaagtt ttccggtaat
    29701 gccttctatt attcaactaa ttcatcttat caccacggga caattttagt tgatactgat
    29761 tttaagaata tggttgaata cttacaggtt tcccaggaaa agatccaatc caaggggata
    29821 gaatctgtga aatcaagagt ggttaactta caggagttaa atccagatgt tacagtcgaa
    29881 caaatgaaga gcgaattaga aaaatacttc aaagaattat atcaggtaga gcctgagcca
    29941 cagactatat caccacatca aaatgaagaa atagctaaac tgcaggaaaa atactcttcc
    30001 tgggaatggc gttacggtca ggctcctgat tttgatatta cttatgagaa ccggtttgac
    30061 tggggcgata tccagttagg tttaagcctc aaaaaaggta caattgaaga ggcaaaaata
    30121 tattcagatg ccatgaatgc tgatctgatt gaaaatattg cagataacct cagtgactgt
    30181 caatttaaaa ttgatgaagt gaataaagca atcagaaatg tagaacatga taaaaaagat
    30241 gaagaagtta ttgatgattt aatagtatgg ttaaaagaaa agagaattta ataatttatg
    30301 atttattctg catgctaaat aagttatata aatgagtaat aagataaaaa aggccgccct
    30361 ttttaggggc ggccttttaa aacttttaat atctatacat ttctgcttca caagaagggc
    30421 agacagctct tacataaccg tctttatctc cttttttggc gctttctgtg atagcttttt
    30481 cttcttttgt atggtattgg gcacagtcat gacagtaaat atcaccacat tcttcacatt
    30541 tatagaatct tccatcttta tggtaagttg ctccacaggc tttacatttt tttgctgaca
    30601 tctttaaacc cccttttgtt aagtacttta tattaattat tataaagtat caaatataat
    30661 aataaatagt aaacactata cacaatttaa attaaaggat tcgattagca ggatttacaa
    30721 tataaatagg gaaatgtata taataacata atatgataaa ggatgatata atataatgga
    30781 acagttaaag gttatgacgg tttttggcac cagaccagaa gcaattaaga tggcacctct
    30841 ggttaaaaaa ctaaaaaaag aagataatat caactgcata gttacagtaa cagctcaaca
    30901 tagagagatg ctcgatcaag ttttagattt atttgaaatt aaaactgatt atgatttaga
    30961 tataatgtct aaccagcaga ccttagtaga tattacggtt aatgtcttaa aaaatctaga
    31021 aactgtcctt aatgaggaag agcctgacct agtattagta cacggagata caacgactac
    31081 ctttatcggg gccttatctt ccttctataa acagattgat atcggccatg tggaagcagg
    31141 cctaaggact ggcaataaat attcgcctta tcccgaggaa atgaatagac atttaactgg
    31201 tgtgttaact gatttgaatt ttgctcccac cataaataat aaggataatc ttttaaaaga
    31261 aaatgtagat gaaaacaaga tatttgtgac cggtaataca gttattgatg ctatccaaga
    31321 gattgtaaaa gaaaattata aatttgataa tgaacaatta aaagatatag attttgataa
    31381 taataaggta atacttttga ccgcccaccg gagagaaaat ttaggagaag gactgactgg
    31441 aattttccag gcagttaatg atattattaa aagtaaccgg gatgctgaag tagtctttcc
    31501 tgttcattta aatccgaaag ttagaaaaac agcctataaa catctagagg ataacaaaaa
    31561 ggttcatcta attgaacctc taggatatct ggaatttgca aatttgatga ataaatcata
    31621 cttaattatg actgattcgg gcggcatcca ggaagaggca cccgggctgg gcaaacctgt
    31681 tttagtttta cgtgatacga cggaaaggca ggaagcaatt caggccggta cggtaaaaaa
    31741 agtaggggta gatagagtaa aaatatttga agaagcagat ttactattga ataataagca
    31801 agcgtatcta aaaatggctg aagcaactaa cccttatggt gatggtaagg ctgtggcaag
    31861 aattatcgac tacattttat ttcactacaa cataaagggg gtaaaacctg agccttttca
    31921 ggttgattaa tatggttaat aatgatgatt tttcaaaaga tagagaggcg atggtaaaaa
    31981 aacagcttat tagaagagga attgaagata agctggtatt agatgcattc cggtcggtac
    32041 cgcgtgaaaa gttcgtacct gaccatgtca aagaatattc ttaccatgat ggtccccttc
    32101 cgattggaga agatcaaacc ataagtcaac cctatatagt agcagaaatg gtcgaagctt
    32161 tagaacttaa tagagacagt atagtactgg aaatcgggac tggttctggc tatgaagcag
    32221 caattttggc agaagtagcc aaacatgttt atacagtcga aagaatccaa agtctggccg
    32281 ataaagcaaa gaaaaaatta gatgaactag gctatgaaaa tatcacaatt gaagttggag
    32341 atggaagttt aggttggccc tctggtgata aagtttttga tggtatagtt gtaagcgcgg
    32401 gagcaccttc cataccgaat agttataaga accagcttaa gacaggtggt tatctggtaa
    32461 ttccggtggg aagccgtgcc ctgcagaatt tatataaagt tagaaaaaac caaaatggaa
    32521 aaatggaaaa acaaagttta ggtcatgtaa ggtttgtacc cttactgggg gaacaggcct
    32581 ggtaaaggag gtaatcgatt tggcagaaaa tattaaagca aaagtcctac aaaattcagt
    32641 aatggtaagt ggacatccca ggagtgggac atcaattact tgccagcttg ttgaaagtgc
    32701 tggagtacat tttccttctg attatgaagg tgatgaatat aatgtgtccg gctactatga
    32761 actagagatc agtaaagatt tatcaaagaa acttatagaa aaagcaatga caattaaaaa
    32821 taccattaag atgaatcagg taatagaaaa gttaaatgaa aacgagggtc tctctggttt
    32881 aaaactggta agagtacccg caatattttt ctacagacat gtggcagaca atcttaaggc
    32941 agtctttatc tttagaaatc cggctgatgt aaaggctagt atgctcagaa ggggcatttc
    33001 aagttttgaa cctgactggt tcgctaataa taatgctcta attgctgcct atgagaacat
    33061 cgaagacagt attttaataa gctatgaaac attactatca gataaagaaa aggttaaagc
    33121 attgttcaac aaaattgatt tagaagttga tgtttcgcta ataaaaaccg gccagagatc
    33181 tcagaaacac agtgatatcg ttttggaaga taaggaaatt aagctgtatg aaaggctaaa
    33241 ggagttagaa gcaaaaaact tttaaaagga gctgatagaa tggatattta tcaatttgcg
    33301 attaattttg agcaggaaaa ccgtgaatat tatgaagaat tagctgagaa aagtgcgagt
    33361 gaaagaataa aaaatgtctt tttagagttg gcttcagaag aagctaaaca cgaaaggatt
    33421 gtaaaacagt taagagataa agaagatgtg ggagacttag aatttgatat tataccaaag
    33481 gtcaataaag tttttgaaga gatcaaggct gatatgccgg acaatgtaat gccaaaagat
    33541 caggttgatg tctacaagaa agcacttaat atggaagaaa aaagcaaaga cttttatgca
    33601 gaaaaagcag aagaagttga aggtgaaaaa gctaaatcag ttttggaaag attggccaaa
    33661 gaagagaaaa aacatgagaa tattgttaga agtattatag aattagtcaa taggcctaat
    33721 acatggcttg aagatgcaga atggtaccat ttagaagact attaatataa tatcaattaa
    33781 gaagcaactg taagcaaaaa aacagttgct tcttctgtct aataatatta caattcaagt
    33841 agaattgagg agggttaaaa gagatggact ttaatatttt gattggggga gaagccggtc
    33901 agggattaaa aacggtggat aatattttag gcaagatatt attcagagaa ggatttaata
    33961 tcttttcctc aaaggacttc atgtccagaa tacggggtgg gcataacttc atgcagctta
    34021 gaattagcga tgaaaaactt tacggccctg ataatgactt agatctatta attgccctta
    34081 ataaagaatc tgtggatatt catagagagc agttaaaaga tgacggtatc atcttaattg
    34141 aaggagacga ggaagtgatc gacggccgta caatcctggt acctgcttct gtaattgcca
    34201 atgatatcaa tccaaagggt attaatacag tatttgtagg tgccgcttta aagataatgg
    34261 atttagattt agacaaggct aagcaggtag taaaagaata ttttgatgaa gatctagtag
    34321 aagataatat caaactgtta gaaagaggtt ataaggcggt tgaccccgtt tatgaaaaat
    34381 taaaagaaaa tgtaagcgat aaaagcgaag aggtatttat agatggcaat tctgctttag
    34441 gttatggtgc gttaacaggt ggattaaggt tttactcagc ttatccgatg tcaccttcta
    34501 ccggaattat gaacttttta gccggacagc aggaaaattt cgatatagtt gttgaacaag
    34561 ccgaagatga gctggctgcc ttgaatatgg ctttaggggg ttcctatagc ggcattagat
    34621 caatgagcgg aacttccggc ggtggactcg ccctaatgaa tgaagcgatt ggtttagcgg
    34681 gaattactga aacccctgta gtggtggcag atgtacagag acccggtccg gctactggac
    34741 taccgacccg aaccggtcag ggagacttac tttttgctat taattcagct caggatgagt
    34801 ttcccttaat ggtaattgcc ccccgtgatc aaaaagacct attctataac ggatttaggg
    34861 ctttaaatat agcagataaa tatcaaattc cggtaataat attgagtgat cagttcaatg
    34921 ctgattcaag taaaaatatc gaggaatttc acttcgattc tttaacaata aatagatatt
    34981 taatttcaga agatgatccg gctgcaaaag actataagag gtacaaattc accgaagatg
    35041 gtatctcacc gcgggcgtat cccggccagt taaaaggaga aacggtctta gtagacagtg
    35101 acgaacatga tgaagaaggt catattgttg aagatggtga aacaaggacc aaaatggtag
    35161 ataaaagggc ccgtaagtta gataaattaa tccaggagga cttacaggaa ccaaaatatt
    35221 tcggagatga agatattgat tatcttctct taacctgggg gtcaagctat gggccgacca
    35281 gagaagcttt ccaacttctc aaagaagatg gtataaaagt tggtctttta agctttaatg
    35341 atgtatggcc tttacccaag gaacagttag aagacctggc ctgtgatggt gttgatttga
    35401 taacggttga actgaattca actgcccaat ttggtacttt aatagcttca gaaacattac
    35461 ttgatgttaa acagagtatt ttaaaatatg acgggagacc ctttaccggt aaagaaatat
    35521 ataatagatt cttagatgag gtgatcgata atggctaata ataaagagtt tggtatttct
    35581 tccgaaacag cctggtgtcc cggctgtggg aactttccac tccgcaaggc tttatcagct
    35641 gctttagaag aaatggaatt agatgtcgac caggtagcta tgtttacagg catcggtcag
    35701 gcggcaaaaa tgcctcatta tatcaactta aatggtttta atggattaca cggccgttct
    35761 ttcccaccgg caaccggtgc acgagtcggc aaccataagc tgaaaataat tgtcgaatca
    35821 ggggacggct gttcctatgg tgaaggtggt aaccacatac ttcacaatat cagaagaaat
    35881 cctgatgtaa cacatttagt ccacaataat caggtttatg gacttaccaa aggacaggcc
    35941 tcacccacta ccgaagaata catgcaaacc gggattcagg ttgaaggtac gaccgctaag
    36001 ccctttaagc ctgtaaagtt tgccgtaggg atgggagcca gttttgtagc gagaagtttt
    36061 gtgggagacc aagatcatct taaagaaatg atccaagcgg ctatgaatca cagagggtat
    36121 gctttaattg atatcttaca accctgtgtt tcatttaata agataaacac ctatcaatgg
    36181 tattccaaaa gggtatataa attagataat aactatgacc cggaagatat cgaccaggcc
    36241 tttaaaaagg caagagaatg gggagataat ataccaatag gtattatcta taaaaacgaa
    36301 caaaagatct ttagagacag attactcttg gatgatgatc aacccctcgt tgaaaaagac
    36361 tttgatcccc aagatgctaa aaagataatc gataacttta aataaatatt aaaaaagagg
    36421 ggggctaccc cctcttttat tataaaggtg ataaaaaatg gatgcgaaag aaagaagaga
    36481 aaagttaatt aataaattaa tagatgaaaa tgaaccggtg acaggtgcaa atctggctga
    36541 tttttttggt gtgacaagac aggtaatagt tcaagatatt gcacttttaa gggcccaagg
    36601 gtatgatata ttagctacct ctcagggcta tatcttgaaa aataatatgg gagttaaaac
    36661 atacagtaaa acaattgcct gtaaacatga catgggtaat gtaaaagaag aactgatgat
    36721 cattgtcaaa catggagcta aaattaaaga tgtcaaggtt gaacacccca tatatggaga
    36781 aattgcaggc atgctcatgc tgcagacttc tcaggatgtc gagaactttt taaataacac
    36841 cgaaaaattt gaggcttccc ttttatcttc tttaacagac ggtgttcatc ttcataccat
    36901 cgaagcaatg aacgaagatg ttttagatga aattgtcaaa gaattgaaag aaaaaggttt
    36961 tttattagaa gaggatctat aatttttttg aaaagggtgt atatacactt gactaatcag
    37021 ctgctgtaaa ttataattta ctggaaagga gtggtaatgt gaataaggag gaattgaata
    37081 aaaagaggct tttttggatt atctggttga tagttttact tctatcagca tattttgttc
    37141 cctttgtatt acttcgagat gtcaatcaga ttgaagcttc ttttctttac tggactattt
    37201 ttgcagtttt agctatcttt tctacaatca aaataacgac ttattggagt gattaattaa
    37261 tgaatacatt ttatgtatat ttagcagtaa taatatatgt attaataggt ttttatatag
    37321 catacaaagc acgccagggt atgaaacaag gggtaattga gtatttttta gataatagat
    37381 caaccagtgg aatagtatct gccttatcat atagtgccac cacttacagt gccttcatga
    37441 tgattgggct ggctggattt acttattcag gtggtgtggg agctttaggc tttgagttaa
    37501 tctatttagc cggtctttcc ttagttgcgt tttttggtcc gagattctgg ttagtaggca
    37561 aaaaatatgg atatgtaact ccctatgaaa tgttgggaga taggtttcaa aataaatgga
    37621 ttgcaattat ttcagccgga gcagcctgtg tatttttgat tccttacctt tctgtgcaat
    37681 taatgggtat cggttatctc ctagaaggtt tatcaggggg cagtattccg tttttagcag
    37741 gtgtcatgat agcggcagtt ttagcaatat catgggccat cattgcaggt atgcgttcgg
    37801 ttacctggac cgactcctta cagtctttga taatgatatt agttagtgtt ttaatccttt
    37861 tttatgtggt atatactcaa ttgggtggat ttttatcatt tttcaaccag ctggaaacag
    37921 agtatgctaa ctttctttca gtacccggac cgggattttt caaattccag acctttttgg
    37981 gtctgacatt accctggttt ttcttctcta tttccaatcc ccaggtgagc cagagattat
    38041 ttatacccaa ttctttaaag gcaatgaaga ctatgattaa agggttcctc atctttggtc
    38101 tcgtttatac tttagtatct gttttatggg gatttagtgc caaactatta ctacctggtt
    38161 tagaacaggc tgacctggcc acacctaccc tgcttggttc cgaacatgta ccgatgttat
    38221 tgggtttaat agcaatgatc ggtattaccg ctgctgcaat ttcaacggtt gactctatca
    38281 tgctgacttt atcatccttg attgtaaggg atttaatgca gtcgcgtgac gaatattcgg
    38341 ataagaagca gcttagaatc ggtaaagcag taatctttgt catcgcagtg attggtatca
    38401 tttttgcagc tcaacagctg gatctaattg ccactttatc agtagcttcc tcagttggtc
    38461 ttatggtagt agtacccacc atctttgggg ctttcttttg gaaaaatgct acggcagccg
    38521 gttctctggc aagtataatt ataggtgctg ttgcagctct ttatttacag tttgccggtc
    38581 ttaaccccct gggtgttcac cccggtgttt ggacctttat cataactgtt gtaacattta
    38641 tcctggtcag tctctttact aacgccccaa aagagaaagc agaggaattt atgggttatc
    38701 tagaccagga gttagcagat aaaaatattc tttaaaaaga ggtaattacg gctatttgtt
    38761 aaatattata aatatacgaa accatttatt tgaggagagg agtagtcaat gcaatataaa
    38821 gatgtgagcc gtgatttgga aaagaaaact aatctattag tggactcaac catgaaatgt
    38881 gtttatggtg ataagcttaa ggatattaaa ggcagcgata aagaagaaaa aagcattagg
    38941 gatatagaat atatcattaa ctacctatca gaagccgctt attttaataa caagggactc
    39001 tttgaagatt tctcactctg gttaaacagc ttatttaaca acatcgactt aggggaagaa
    39061 gtatttaaaa ctacatataa atgtataata aaacaaatag agcatgaatt cgatgaaaag
    39121 gttagtaatt atctaaataa tataataaga gaatctatgc aggaagcatt aaataataaa
    39181 gaagagattg catcttttat aaagaaagac aatccccatc aggagtatgc gaaaagatac
    39241 ttagacttac ttttagaaca taaaaaggaa caagctagta agctaataac tgatgaagct
    39301 ctagatgaga tgggtattgc tgatatttat ttaaatatat ttcaaccagt tcaaaaagaa
    39361 gtggggagac tttggcatgc taacaaagtc tcagttgcaa aggaacatta tattagcagt
    39421 gtaacacagc taataatgtc ccagttgtac ccccactttt tatctattgg aggtaaaaaa
    39481 ggcagtgttg taaccactgc tgtcggaaat gaactacatg aaatcgggat aaggatgatc
    39541 gcagacctat tagaggttga cgggtatgat acaatacact taggagcaaa tactcccaat
    39601 accggtatca ttcaaacctt aaagagcaat aaaacaaagc ttctcggggt ctcggttact
    39661 ctccctctcc acctcaaaga acttgataag ttaattacta tgattagaga tgaacgcgaa
    39721 ttaaataatt taaagattat gatcgggggc tgtgctttta atagaaatcc ggaattggtt
    39781 aatgaatttg atgtagatga atacagtaaa gatgctcaag atgctgtaaa gaaagctaat
    39841 attttaacgg gagtggtgaa ctgatgggtt ttgttgtact tacctccccc gaattgaaga
    39901 taaaaaggat cttatataac gacacttctt tttctttttt aaaagaagat aggatcttaa
    39961 cttcttttgt caataagaat gagcgttcta aactgctgga ctttgtgcat aaaatccagg
    40021 ctgataaggc agagtttggc tatgagatcc acttacaaac taaagaagat agtattttat
    40081 ataatttttc cggtattaaa gcagaaaatg attcaatact cattttagct tcccagttca
    40141 aagaggaaat aataaaccag tatgaccact tcatgaaggt gaataaccaa tatataaata
    40201 agattagaag gctgttaaaa aatcagatta agtctgataa tgaggtggta ctaagcggtg
    40261 acagctctga tatatataac cagattagcc aaatcaataa cgaacttatg gaagtgcaga
    40321 gagaacttac caaaacaaat aaagaattaa aatggcagaa agaaaggtat tttgccaccc
    40381 ttaaaagtat tggtgaggga gtcattgctt taaatgagga aaaaagaatt caattcataa
    40441 atgaagcagc ccaagctata ttgggtataa aagataatat aaaaggtgat tatctacaag
    40501 cacaggatat taagtttcta gaccaaaatg aaaatgatat attagagaac ataattgagc
    40561 gggtatgtaa tactggaggt ttaataaaaa aagaagacct ccttatgaaa acaaaagaaa
    40621 aggaagttcc cattgattta actgtttctc cgattagaat aaatgaagaa aagttattag
    40681 gtatagtaat tgtaataact gacataacct taaagaaaaa acatgaagaa aagttaagaa
    40741 agttggcggt gaccgatcgt ttaaccaata ttatgaaccg ccggatggga ataaaatatt
    40801 tagaaaaaca aattgaaagg ttaaaaagag aagatttccc tttaacggtt tgttttattg
    40861 atgtcaatgg tttaaaaaag ataaatgata attacggcca tttagaagga gaccatcttt
    40921 taaaagaagt ggccgaaatt ttggaccaga atgtaagaag ttcagatgtg gttgctagaa
    40981 ttgggggaga tgaattttta atcatctttt ctaataatgt gatttcagaa gtgcaagagg
    41041 tttgggatag gattgaagag gaagttaaca aacggaataa aaatatggaa aaaccataca
    41101 aaattagttt aagccacggc tttgctcaaa aggtaaaaga cgatggttta agtctcgatg
    41161 atctaataga taaagcagat caaaagatgt ataaggataa acaacagtat tatagtaata
    41221 aagaataata gtcttggggt gaatttaata tgaaaaacaa acaatatata gcatttttaa
    41281 ttttaataat attatttagc ttttctctgg ttggacaggc tgttgataca gctgttacaa
    41341 ttgtgcctca aaaacagttt gtaaaggcag tagctgaaga caaaagtgaa gttaccgtga
    41401 tgataccacc cggttacagt cccggtaatt atgccccccg tccttctgaa ctaacaaaat
    41461 tttccgaagc caagatatat ttttcaatgg gagttcccgc cgatgtacaa aatatcttac
    41521 cgaaagtaaa tcagtttaat gctgatataa agattattaa attagcgaat ttggtgggtg
    41581 aaaagtataa aaaccgtact tttcccaatg gaagtcgcga tccccatatc tggttatcac
    41641 caaaaagggt tatctatatg gttaaaataa taagagatga actaattaaa ctggattctg
    41701 acaatgaaga attttatcga gacaatgcgg aagaatatat tgaaaaaata agagccacag
    41761 atcaaaagat aagggatatc attaaggaaa aagataataa ttatatgctg gtttaccatc
    41821 cctcctttgg atattttgcg gaagaatacg aacttaaaat ggtttcaatc gaagagggtg
    41881 gcaaagaggc aactgccaaa cacttacagg agattataga tttcgccaag gaaaatggga
    41941 ttaataaaat attttatcag gcagagattg acagccgtca aacagaggca atagcggaag
    42001 agatagatgc agagcttatt aaactagatc ccttggcaga agactattta accaacctaa
    42061 ttgaaatggc taaaaagatt agtggagttg aataagtatg gaagatgaaa agataataga
    42121 agtaaaagat ttgtatttat catataatca ggttaatgta ttagaaaatg tgcagttaac
    42181 tgtctataag gggcagtttt tagctataat tggtcccaac ggaggaggca agacaaccct
    42241 gctcaaaata atattgggtt tgattgaacc tgatagcggt gaggttaaaa ttaaaggaca
    42301 tgatattaaa aaaggacgca aactggtcgg ttatgtcccc cagatttcgg actttgataa
    42361 aaactttcct ataaatgttc aggatgtgat cctgatgggc cggatcaccg accaaaaagg
    42421 gcttttcttt aattatagtg aagaagataa aaaaatagcg gaagagataa tgcatgattt
    42481 agatatctat aatattaaag accgccagat cggaagatta tccgggggac aactacagag
    42541 ggttttaatc ggtcgggctc tggcagctga accagagatc ttgcttttag atgagcctac
    42601 ggccagtctt gatgcagagg cgagatcaaa tatatatagt attttaagag agattaataa
    42661 agagattact attattgtag ttacgcacga catttcagct atttcatctc actttgatag
    42721 tgtagcctgt ttaaacagga atctacatta ccatggtgat aagaatatta ctaaagaaga
    42781 tctagatcaa gtttatggtt gtccggttga acttattgcc cacggagttc cccatcgggt
    42841 attagcagac catgaagggg atgatcataa tgattgaagc aattttgcag tataatttcc
    42901 tacaaaacgc ggtttactca gctttgatgg ccagtgtggt ttgtgggatt attggtacca
    42961 ttataattga aaaaaatctc gtgatgatga gtggagggat agcacatact tcatttggtg
    43021 gggttggctt gggttattat cttaatattg agcccatctt tggggcctta ttttttgccc
    43081 ttttatcctc agtaattatt gtcaggatca gaaaaaggac cgaagttcat tctgatacct
    43141 taattggtat catgtgggca atgggcatgt ctttagggat aatgtttatt tcctttatgc
    43201 agggttatcc acccgatatg tcttcttatt tatttggaga tattttgact gtgaccgata
    43261 tggatataaa tataatgttg attctggtca tcgtagtggt ggccgccatc acggccttat
    43321 ttaaccagtg gaaatcttat ctttttgacg aggaattttc ttatgtgaga gggattaata
    43381 cagaatttta tgagtacttt ctttttattt taattgccct tacagttatt gtcttaatta
    43441 gggtaaccgg tataatctta ataatcgcct tattaactgc tccgccggcc atggccaaac
    43501 ttttcaccta tgattttaaa aagataatat atttagcaat tggttttgga gtaattttta
    43561 caatgaccgg actctggctt tcctatatgt taaatattcc ttcgggagca acaattattc
    43621 tcctaattgg ggttacttac tttatcatgg cttctataac gcagagattt aatgtaggca
    43681 gctgactgat caagcacttt ctgatttatt tatttcatct gatttaacta gggcatattt
    43741 ggtggcaaat gagccgaata tttctgtcag ggcagctgtg aacaatagaa tattaaagat
    43801 caactgacca atgttggcct gggtaaattc ctgctggacg gtgtaggcta aagcaattgc
    43861 taccccactc tgcggtagga ggcccagccc tacatatttt tttatgtttt cagaagcatt
    43921 ggaaagaata ccgcctaaag aagctccccc tatttttccc actactctcg tagcagtata
    43981 gaccaaagct aatatgataa aaggtatatc aatacccgag aacctgattt cagtacctgc
    44041 caaaataaag aacatcgcat ataaaggtat tgtaagtgtt tctacatagt cggctatatt
    44101 taagttcttg ctggcaaagt ttctgtaagc aaaaccgatg gccatattgg taatgagagg
    44161 cgataaattc aaaagagtag ccgcggccag cccacttact aaggttgcga tcagggtaag
    44221 gactttttta gttttatctt catatttttc ctgtaatttt accgagatat atccgatcaa
    44281 aatccccgct ataatagagc ctaccacttc tacgagaggg acgagtatca tgtggtttat
    44341 gcttagactt gctcccccac tgtactgatt atatgctatg ggactaacta tactaaataa
    44401 aattaaggcc agagcgtcat ctaatcctac tacagccata atcattgagg tgactggacc
    44461 ttctgcatta tattctctta ttaccataac tgtagcagcg ggagcggtag cggaagcaat
    44521 cgcccctaaa agcagggcaa tatataggtc gtagccgatt aaaagtatac ctataaatac
    44581 cataaaaaag gcccctaaac cctgaatcaa tactatatag gtgatactct tacctaactt
    44641 cttgaagagt ttaagtgata attcactacc aattgtaaag gcgataaagc caagggctag
    44701 ggttgttaca tactcaaaac tgtttataat ataggcatac cagactttat attccaattt
    44761 taaaaatgga attgcattta ttacctcgat actaagcagg atacctaaaa agacataacc
    44821 gactacaatc ggaatattat accgtttggc aaaaataaca ataagaagag cggctagaac
    44881 aaaaaaggcg attagataaa gttctttatc actaacctga tgttctataa accactcttc
    44941 atatttataa atttgagata acatctctgt atccataatt ataccttctt tacacacaaa
    45001 attagattaa gcgaatcttt taataatcac attctccctc agtacataat agataatata
    45061 ataagtctcc ccgggagata tagcccctta agtatccttc atcatttgta acaaatagat
    45121 aagattcctt attgtttaac atgatatcag caatcttatc catgttatct ctttccagga
    45181 ccgtatctgt cttagtaacc atatactctt cgacttttct atactttata tcacttaagt
    45241 tttcggcggc ctgatttcta tcatataaga ttcgctgact attgataaaa ccatactgtt
    45301 cggggataat attttttagg atattcttac tataaatcgt gcctataaag tggttttctt
    45361 catctacgac cggtaagata ggcatttcgg ttttatgcat ataaactatc gcatcttcaa
    45421 cggtatcttc atatgagact gaatttaagg acctcatcat ataatttttt acaatagcca
    45481 tctttagacc tccatttcct ctatgataat attgtcaaca tttttttcta tttcttctct
    45541 attgtgaaga aatactgttt tatgttcagc aatatctaag gcagctgctg cagcataacg
    45601 gttcgcttct atgaaatcaa agccgttttg atagataaaa tcaaagaaag ccgcattata
    45661 ggcatcacca gcccccagta agtttctaat acagtattca cggggtgaaa gcatatagat
    45721 ggtatcatca ttatcaaaaa gtaatctatt agtgtggaag ggcattatta catattgagc
    45781 cccttctttc tgcagctttc tacccatctt tatatagtca tcaagttctt ctaccttgac
    45841 gtccaatatt ttatgggtat gtttaaagta gggaacaaca aattttggtt ctgcttttag
    45901 agcccgattt agggcttccc cactggcttt aagatagaca tctttatcaa gttcattaca
    45961 gatttctact aattcattat agatatcaaa acttactccg gatgggattg aacccgcgat
    46021 cattactaag tcactttctc ggatactcct tttaaagcgc ttttttaatt cttttacact
    46081 tctttcactt actgaataag tatagtcgtt atacagggta ttactgggtg gattttgtgc
    46141 aattatcttt atgttgtttc tcatttcttg cgaggtgtac acatagtttg tggtaacctg
    46201 gaaatcctgt accatctttt cgaagaaaag gccctgttta ccacctataa aacctatatt
    46261 ctgtacatcc gaataaccca agttttttaa attaatagct gctattaatc cttttcctcc
    46321 gggatagact tttaaatttt cattatcata aatatattga tctttcattg gtttatgttc
    46381 atcgatgaaa tattccctat caatggaggg atttaaagtt acagtagtta tcaataatat
    46441 cacctctgcc ttatattcat tattattata tttctataaa taagtcctat ttccttttat
    46501 ttaaataatt gagagctatg atatttaaaa taattggatt agtactgaat ttaaatcggt
    46561 caatttaaaa aaattaagta attttatcaa taaaatcgaa ggaattagag aaaaattaac
    46621 gaagttatat tgatgggagc tgtaaaaata ttcaaacttg tatacggaaa gaggagggat
    46681 atttgcttgc ataaatacct atatatggta taattataaa aattcatgag aagaaaggag
    46741 tagtgagtag ggagaatgga atatagttta cctaatttta cttggttttg gctattggca
    46801 ccgaatttat tgattgtttt actgtcactc ttgcgtttgc ttaaaagaag agggggtgac
    46861 agttaatgga tcctgtatta gttacttttt ctatttatat tggcggtatg cttttgatcg
    46921 gttattatta ctaccgccag aatgaaaaca tatctgatta ctttttgggc ggccggacct
    46981 taaatccctt tgttactgcc ttgagtgcac aggccagtga tatgagcggc tggcttttaa
    47041 tgggttttcc cggtgcgatt tatgcgggtg gttttaatgg aatgtggatt gggatcggtc
    47101 tggcaatagg aacctacctt aattggcagt ttacagctca gagattgaga aggtatacgg
    47161 agatagtaga ctctataaca attgccgatt attttgaaaa caggtttaaa gataacagta
    47221 gagctttaag aatagtaact gctctagcta tattaatatt ttttactatt tatgtatctt
    47281 caggattagt ggccggtggt ctgctttttg agaatatatt aggtgtgaac tatcagctgg
    47341 ctgtagtcct ggcagtcata attattggtc tttatacttt tctaggtgga tttttagcgg
    47401 ttagttggac tgactttgta cagggatctt taatggttat tgccttatta atcgttccga
    47461 ttgcattaat atcaattagg ggtggattaa cagaagtttg gggccagatt gccagtatta
    47521 acccggactt attacatgcc ggtaagattg tctcatatga gtttggatca gaagcgcagt
    47581 ggataacaac ctcagaaaat atgagcttta tctctctcat ctctttattg ggatggggtt
    47641 tgggatactt tggtatgccc catattttgg ttaggtttat ggggattaaa tcagcgaagc
    47701 agattccaat atcccagttt gtgggagtaa cctgggttgt tgtcagttta attggcgctg
    47761 tctttgtcgg aattttaggt attattgccg cacccgaggc tttagaaaat acagaaaccg
    47821 tctttttagt acttttacag aaactattta atccctgggt agcaggtgtg tttttagctg
    47881 cggtcctggc tgctattatg agtacgatag attcacagct attagtttca tccagtgccc
    47941 tggccgaaga cttttataaa tcacttttca ggccgggcgc cagccagaaa gagctgctct
    48001 gggtgagtag actggctgta ctatttatca ccgtattagc cctatttttt gccctatcag
    48061 ggggaacaat tcttgatata gttgcctatg cctgggctgg tttaggagca tctttcggtc
    48121 cggccatctt atttagcctg ttctggaaga agacctcccg ggacggggta ttaggcggaa
    48181 ttattacagg tggtgtggta gtgataattt ggaagaactt ttttgcctat acaggacttt
    48241 atgaaataat accgggcttt ttcctatcgg ccctggtcat ttatgtaata agttactttg
    48301 gtccagatcc attaccagag gtagtagatg aatttgaaag agctatgaag cccttaatta
    48361 atgaatagat aagcgagggg gagtcaaatg cgaattaagg agcatccaat cttagatttt
    48421 gatagaaaag aagagataac atttacttat aatggaaaag aaattaaagc atatgaagga
    48481 gaaactattg cagccgctct ttatgcagct ggcattagag tgcttaaaac aagcagtaaa
    48541 tatgaaagac cgcgcggctt attctgcgca attgggaact gttcttcctg tcatatggta
    48601 gtagacggga cacccaatgt aaaaacatgt ataacaaaag ttgaagaagg tatggtagtt
    48661 gaacaagagg tcggaaaagg tgatgtaagt gattaatact gatgttttag taattggagg
    48721 gggacccgcc ggtatgacag ctgctttaaa agcagctcaa aagggtgcct cagtcattat
    48781 tgtagaaaaa taccacgaac tgggcggaca gctgataaaa cagacccacc gttttttcgg
    48841 ttccaaagaa gaacaggccg gtattagagg tattgccatt gcagatgaat taactgaaaa
    48901 gattgaaagt aatgataaga ttaaagtttt aacagaggca accgcccagg gatattatga
    48961 agataaagta attactgttt cttatcagga acagtttcaa aagattaaac cgaaaaaagt
    49021 aattattgca accggagctt cggaaaagat gttagccttt gaaaacaatg acctgcctgg
    49081 tgtatatggg gcaggtgctg ttcagaccct gatgaatctc tacggaatac tgccgggcga
    49141 aaaagttgta atggtagggg ccggtaatat cggtttgatt gtaagttatc aactactgca
    49201 ggctggagtt aaagtggtag cagtaataga agccgctcct aaaatcggcg gttatttagt
    49261 gcatgcttcc aaaataagga gagcgaaagt tcctatctat acaagccatt ccataaaaag
    49321 ggccatcggg gaaaaatcgg tggaagcagt tgaaatggtc aagttagatg agaactggaa
    49381 catggtcgaa ggatctgaac agataatcga agccgatacg gtctgtttgg cggtgggatt
    49441 acgcccgctg accgatctat tgatacaggc cgaatgtgag atggcctata tccccgaatt
    49501 ggggggcgat gtgccgctga gagatgagaa tcttaagaca accaatgaac agatctttgt
    49561 agccggtgat gcagccggaa tagaagaagc tacggctgcc atgttagaag gtgaattagc
    49621 cggtctaaaa gcggtttctg aaattggtta cacagatccc gatttatttg aagagctcaa
    49681 tcgactaaaa gaagaactaa aaaaattaag aaacggcccg gttggaaaag tcattcgaaa
    49741 agggcttaaa aaagcgaggg gaaaagaaga tgctggaaaa aacaggtatt cccacatccg
    49801 aggatataga taatataaaa ccagacgaag agagactcaa agaaggtcca gtagcagtta
    49861 ttgaatgttt caaagagatc ccctgtgacc cctgctttta cagctgtccc tttggggcaa
    49921 ttaaagagtt tgaagatata aatgataaac cggaattaat cacagagaaa tgtacgggct
    49981 gtggaaactg tatagcaagc tgtcccggtc tggcaatttt tgtggtcgac tataataaaa
    50041 agacagttag tttaccgtat gaatttttac cactaccgga gaagggtgag catgtaaaag
    50101 gcttagaccg gggtggtagg gaagtctgtg aagcagaagt agttaaggtc agggatccta
    50161 aaaagaatga taaaacggct gtcatcacag tcaaagtacc ggaagagtat ttaatggaag
    50221 taagaaatat agaagttggt gatttagatg aatgatgata ctattatttg ccgctgtgaa
    50281 gatattacct taaaagaact tagagatttg atcaaggaag gttacacaac tttagatgaa
    50341 attaaaaggg ccctgcgttt gggtatggga ccctgccgcg gtgatatctg cctggaactg
    50401 gccgcccgcg agatctgtcg gctgacagat aaaacgatgg aagatataaa gatgcccaac
    50461 aataggcctc cccttaaagg tataaaaatg ggcgaattag caggtgatgt cgatgaaaaa
    50521 taaagcaaat attgtaatta taggtggggg agttatcgg
//
LOCUS       DATGCJ010000063         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_141641, whole genome shotgun sequence.
ACCESSION   DATGCJ010000063 DATGCJ010000000
VERSION     DATGCJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141641"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VKN64_03550"
     CDS             complement(<1..200)
                     /locus_tag="VKN64_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA59345.1"
                     /translation="MLSLKEVTKVYENNNKKAVNNLNLEVNKGEIFGFLGPNGAGKTT
                     TIRMITGILDITEGEILVNGFN"
     gene            complement(250..750)
                     /locus_tag="VKN64_03555"
     CDS             complement(250..750)
                     /locus_tag="VKN64_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59346.1"
                     /translation="MKLIGKILVGFALIIVLILAGGALYIDIPVFDNVNEFDNYLASY
                     SQVKYNVAEEFETLNAFNKDSLINKEVNKLKVNGEEVIVSYFRYKDPFTARSSINNYK
                     EMNTGNFQVKTMEINIPFYGKYWIQTSQGITSISWTRNKRVILIRSNSREIAKIVEAD
                     IKSYFK"
     gene            complement(769..1539)
                     /locus_tag="VKN64_03560"
     CDS             complement(769..1539)
                     /locus_tag="VKN64_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59347.1"
                     /translation="MLFSLIKNEYYKLFRKKKIYIFAMIIAAMAFIPVIINTIEEAGI
                     NFTVNTLPLENLSWSTEMIIPIFIAVLVADLITNEYQEGTFKLSLVSSVTRMELLAAK
                     IITLVSVITLLVLFTMMVSYLSGAIFLDWGIPFQFNNVTLTETQGILYTFGLYFLAVV
                     PLSAFGILIMFLGFKLESGGSVVGLTIGIFFIMNIIRQLSKLMRPLVLSNYFNTGYLL
                     SSDGMSDKLIILIIVSLVYGLAFFGLSVSDLIKKDIVK"
     gene            complement(1529..2446)
                     /locus_tag="VKN64_03565"
     CDS             complement(1529..2446)
                     /locus_tag="VKN64_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59348.1"
                     /translation="MDNIILKIENLEKNFGKLKAVKNVNLEVKKGDVFGFLGPNGAGK
                     STTIRMVLGLVNPDKGRIIIDGLEIQKDYKEALKKVGTMVETPSFYEYLTGYENLKLT
                     AKLYQGIKHVDIIKILKKVGLKDRKDNKVKTYSVGMKQRLGLARALINKPQLVILDEP
                     TSGLDPQGKKEIRELIKGLAETEDVTFFICTHQLNEVEQICNRVAILQEGKIIANRHI
                     NKKENEKWIEIYTKQKNEAKMILKQDEKIKNMKISKNGLLIYTKETQIEKINSLLNQR
                     GIKVKYIIPEMESLEEYYLKETGGKFDVI"
     gene            complement(2534..>3771)
                     /locus_tag="VKN64_03570"
     CDS             complement(2534..>3771)
                     /locus_tag="VKN64_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA59349.1"
                     /translation="IRDNINLLKNPQEFKSSIDSLLTEYQGELQIIDPETDMIVLDTK
                     GSEQREFDYSNLNINSNLDSDYFTYLNEVETEQGKYIYSLIFNRTSMFQSVQGTVMRY
                     VALGVIISILLLGFLVYYFSRIISRQILIPLEELNEATKNIAEGNLNYEIDYCGDNEL
                     GNFCQAFETMRLKLKESLEKQSLYENNRKELIASISHDLKTPITSIQGYVEGLIDGIP
                     KDEDSYNKYLQIIKDKSIKLNHLIDDLFYFSKLELDKLNIDTRKYSSREAFNEILHPH
                     VLEFEEINQKLIIEKPIPEVKIKVDKKRINQVVDNIIKNAREFMKEDGKIEINFDVGQ
                     KYFNVYITDNGVGIKEEDQEQIFEKFYRGEKSRSRQFGGTGLGLAICREIIEAHDGKI
                     GVESLRGEGSTFYFKLPFA"
BASE COUNT         1187 a          713 c          424 g         1447 t
ORIGIN      
        1 atattaaacc cattcaccaa tatttcacct tcggtaatat ctaatatacc ggtaatcatt
       61 ctaatagtag tagtttttcc ggccccattt ggccctaaaa atccaaatat ttctccttta
      121 ttaacctcta gatttagatt attaacagcc tttttattat tattttcata tactttggtg
      181 acctctttta aacttaacat tgatctccct ctttcttata attttttact tgctctttct
      241 tataattttt tacttgaagt atgatttaat atctgcttct actatcttag caatttctct
      301 cgaattactt ctaatcaaaa ttactctctt atttctggtc caactaatac tggttatacc
      361 ttgactggtt tgaatccaat acttgccgta aaagggaata ttaatttcca tggtcttaac
      421 ttgaaaattg cctgtgttca tttctttata attattgata ctgctcctgg ccgtaaaggg
      481 atctttgtat ctaaaatagg aaacgattac ctcttctccg tttactttaa gtttattaac
      541 ttctttattg atcaagctat ctttattaaa agcattaagt gtttcaaatt cctccgctac
      601 attgtatttt acttgactat aacttgctaa atagttatca aattcattca cattatcaaa
      661 aacagggata tcaatataaa gggctccccc ggctaaaatt aatactatta ttaaagcaaa
      721 tcccactaaa atcttaccaa taagtttcat caaaagttcc tccttttatt attttactat
      781 atccttttta attaaatcac ttacactcag accaaaaaat gctaaaccgt aaaccaaaga
      841 tacaattatt agtattatca atttatcaga cataccatct gaacttagta agtaaccagt
      901 gttaaaataa ttacttagga ctaagggtct catcaatttc gataactgcc tgatgatatt
      961 catgataaaa aagatgccaa ttgttaatcc tacgactgaa ccacctgatt caagcttaaa
     1021 gcctaaaaac attattaata tcccgaaggc tgaaagcggc accactgcta aaaaatatag
     1081 accaaatgta tataagatac cctgggtttc agttaaagtc acattattaa actgaaaagg
     1141 tatgccccaa tccaaaaaga tagcaccact tagataagaa accatcatcg taaataacac
     1201 caataaagta attacggata ctaatgtaat tatttttgca gctaaaagtt ccattctggt
     1261 cacactgctt actaatgata atttaaaagt accttcctga tattcattgg taattaaatc
     1321 agccactaaa actgcaataa agatcggtat tatcatttca gtactccagg ataaattctc
     1381 aagtggtaaa gtatttacag taaaattgat accggcctct tctatcgtat tgatgattac
     1441 cggaataaaa gccattgcag ctattatcat tgcaaatatg tatattttct ttttcctaaa
     1501 aagtttataa tactcatttt ttatcaaact aaataacatc aaacttacct cctgtctctt
     1561 ttaaataata ttcttctaat gattccattt caggtataat atacttaact tttatacctc
     1621 tttgatttaa taaggaattt attttttcta tctgggtttc ttttgtataa attagtaatc
     1681 catttttact tattttcata ttctttattt tttcatcctg ttttaagatc atttttgctt
     1741 catttttttg tttggtataa atctctatcc atttttcatt ttcttttttg ttaatatgtc
     1801 tattagcgat aattttccct tcttgcaaga tagctacccg attacaaatc tgttcaactt
     1861 catttaactg gtgtgtacag ataaaaaatg tcacatcctc agtttcagct agacctttta
     1921 taagttctct aatttccttt ttaccctgtg gatccagccc acttgttggt tcatctaaga
     1981 taactaactg aggtttgttt attaaggctc tcgctaaccc taatctctgt ttcataccga
     2041 ctgaataggt ctttacctta ttatcctttc tatcttttag ccccactttt tttagtattt
     2101 ttataatatc tacatgtttt attccctgat ataatttagc agtaagttta agattttcat
     2161 aaccagttag atattcataa aaactgggcg tctctaccat ggttccaact ttctttaagg
     2221 cttctttata atccttttgt atctctaatc catcaattat aatcctgccc ttatccggat
     2281 taacaagccc taataccatt cgaattgtag ttgatttacc ggctccattg ggtcctaaaa
     2341 acccaaatac atctcctttt ttaacttcca aattcacatt tttaaccgct ttcagtttac
     2401 caaaattctt ctctaaattt tcgattttta aaattatgtt atccataaaa aaatacctcc
     2461 agtatttgat cttacattta taatactaga ggtatcttaa atcacaatta tgaaattatt
     2521 aaaattttat taactatgca aaggggagtt tgaaataaaa agtggaacct tcacctctta
     2581 ggctctctac acctatttta ccatcatggg cttctattat ttctctacag attgccagtc
     2641 ccaacccggt tcctccaaat tggcgggacc ttgatttttc accgcgataa aacttttcaa
     2701 aaatttgttc ttggtcttct tcttttatcc ccaccccatt atcagtaata tatacattga
     2761 aatacttttg gcctacatca aaattaattt ctatttttcc atcttctttc ataaattcac
     2821 gcgcattttt tattatatta tctactacct gattaattct ttttttatca accttgattt
     2881 taacttccgg gattggtttt tctatgatta gtttctgatt gatttcttca aactctaata
     2941 catggggatg caagatttca ttaaaagctt cgcgactgct gtactttcta gtatcaatat
     3001 ttaatttatc taattctaac ttcgaaaaat agaaaagatc atcaattaga tggttaagct
     3061 taattgattt atctttgatt atctgtaagt atttgttata agaatcttca tcttttggta
     3121 taccatcaat aagaccttct acatatccct ggattgatgt aataggagtt tttaagtcat
     3181 gggaaatact ggcaattaac tcctttctat tattctcata taaagactgt ttttctaatg
     3241 attctttcaa cttcaagcgc atcgtttcaa aggcctgaca aaaattacct aattcattat
     3301 caccacagta gtctatttca taatttaaat taccttcagc aatattttta gtagcttcat
     3361 ttaactcctc taacggtatc aaaatctgtc ttgaaataat tctactaaag taatagacca
     3421 aaaaacctaa taaaaggatg gatattatta cgcccagagc cacatatctc attactgtac
     3481 cttggaccga ttgaaacatt gaggtcctat taaagattaa agaatagata tatttaccct
     3541 gttcagtttc tacttcatta agataagtaa aataatctga atccaagttc gaatttatat
     3601 ttaaattact ataatcaaat tctctctgct cagaaccttt agtatctaaa acaatcatgt
     3661 cagtttcagg atcaataatc tgaagttcac cctgatattc agttaataaa gaatctatag
     3721 aacttttaaa ttcttgtgga ttttttaata aatttatatt atcccttatc c
//
LOCUS       DATGCJ010000064        10444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_632621, whole genome shotgun sequence.
ACCESSION   DATGCJ010000064 DATGCJ010000000
VERSION     DATGCJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632621"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /gene="murF"
                     /locus_tag="VKN64_03575"
     CDS             complement(<1..634)
                     /gene="murF"
                     /locus_tag="VKN64_03575"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMA59350.1"
                     /translation="MFKYKVKEIIRATEGKLLKGNRKQILKGVSTDTRKLNDEDIFIA
                     LQGENFDAHNFIDDNLAKQVKALVIQENIEVECENIIKVDDTTKALQAMAKFHRANFK
                     DVKVIGITGSSGKTSTKDILFNLLNKKYNVKRNEGNLNNHIGVPLTLFRLNGDEDFFI
                     VEMGMSDKGEISLLADISDPQIGLITNVGRAHIEFFDSVEDIARAKGELIE"
     gene            complement(627..2099)
                     /locus_tag="VKN64_03580"
     CDS             complement(627..2099)
                     /locus_tag="VKN64_03580"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMA59351.1"
                     /translation="MYLDKVIDNLDVEYIKGNKMVEINELRYDSRNIEPGDLFICVQG
                     FKTDGHKYIDAALKKEANAVVIDKDLDNYDENITYVKVPNSRIAMPQLAKNFFENPLQ
                     EIDLIGVTGTNGKTTTTYLIKEILNHQGYKTGLIGTIEVFDGEKSIDASRTTPESIDI
                     YRYLAEMRDKGVKYTVMEVSSHALALHRVDTMDFTAGVFTNLTQDHLDYHETMEEYAG
                     EKAKLFKKLKTNGKAVINNDDDFSQFFKDSTTNEIITYGIKNVSDLRAEDLELSLEGV
                     KFTLNNLRYELNLTGKFNIYNSLAAVGAAKSLGISEELIKESLESVKGIPGRFETINI
                     GQDFTVIVDYAHTPDSMINVLEAVKHFEHKDIIVVFGCGGERDKGKRPIMGQIAVEKG
                     DYVVVTTDNPRSEEPEIIIKEILKGIKSSQYDTPYTVEVEREAAIYEAINKARKDDVV
                     VIIGKGHETYQVFADKTIDFNDKVVAKKALKEKMSEFKDV"
     gene            complement(2106..4187)
                     /locus_tag="VKN64_03585"
     CDS             complement(2106..4187)
                     /locus_tag="VKN64_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HMA59352.1"
                     /translation="MDDNKVDNDRKKRIKILFFIFTFIFLLLIARLAWIQIINNEEYY
                     NKALKQRMKELKVNPDRGKIYDINGKELAVSIKSKTVVGLPKQISHPTQTAGQLADIL
                     SIGYETLLNRLKRDAYAVYLERKIPDQKVQEIKDLKLDGITFIDESKRIYPKDKLASQ
                     LVGFVGVDGNGLEGIELTYNKYLTGNAGRILTERDAAGKMIPEGIIDYIPEENGSNLY
                     LTINEAIQHSSERELAKAAEEYKAAGGTIIIMEPETGNILSMANYPTYDPNNFSQYKQ
                     KYWRNRAITDNYEPGSTFKIITTASAIEEGVVNKNDVFFDSGKVKVEREYIQCWEEEG
                     HGKELFSQVVQNSCNPGFVQVGLRMGKDSFYKYIDAFGFGQKTEIELPGEAIGLIPDY
                     EQIGDIELATISFGRGISVTPIQLITAVSAVANDGVLMKPNFIDRITNQKGKVEKRLS
                     PTEVRKVVSSRTAHKTLNLLENVVSNGTGVNAYIEGYRVGGKTGTAQYYNQDKYDSSF
                     IGIFPVNDPQFVVLVVLYGIEGNTYYASQTAAPLFKKIANDLIRYYKLPSQEKQKEPP
                     VNETLKLADYSRQNLYNVKNTLKELGLEYKIVGNGNEIIDQIPKAGSEVYKNSTVFLF
                     TEKSDSIAASYRIMTPDFRGMEKKEALNLAEKMGIKLKFQGQAGQVVGQSVDPGLRID
                     DDKEVILKLKG"
     gene            complement(4198..4662)
                     /locus_tag="VKN64_03590"
     CDS             complement(4198..4662)
                     /locus_tag="VKN64_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HMA59353.1"
                     /translation="MFKRDNKKGYNNNTNNYNHYENGEYAEQNNEDKKNIYIYLILLT
                     ICAIILLVYISYTLRINNLSYRIDQMQNEIQALEEKNHKLGIKLSNASSLSQVDQLAR
                     SELNMVEPDSMQTLVLKTEEVEVANTPERSYFLSEVTDFITNLGTARAYSPE"
     gene            complement(4681..5610)
                     /gene="rsmH"
                     /locus_tag="VKN64_03595"
     CDS             complement(4681..5610)
                     /gene="rsmH"
                     /locus_tag="VKN64_03595"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMA59354.1"
                     /translation="MHNNHKPVLLEETIEYLSIKKDGIYVDGTLGRGGHTAGILENLG
                     EKGLVIALDRDKDAIEAVKDSIESSNLILEHANYINIGDVLDKHEIDEVDGMIFDLGV
                     SSPQLDNSDRGFSYKNNGPLDMRMNRDQKLTAADIVNNYRKDKLEEIIYDYGEENWAA
                     RIAEFIVAYRKKKEIETTFELVDIIKAAIPASARRSGGHPARRTFQALRIATNNELNQ
                     LEDMISSATNRLKKGGRICIISFHSLEDRIVKHKFKDLASDCVCPPELPVCVCDKEPL
                     VKIITKSPIRATEEEVEENYRARSAKLRVAEKI"
     gene            complement(5625..6095)
                     /gene="mraZ"
                     /locus_tag="VKN64_03600"
     CDS             complement(5625..6095)
                     /gene="mraZ"
                     /locus_tag="VKN64_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMA59355.1"
                     /translation="MVDGGKIGVKVINMLIGEYLHKMDSKGRVIIPAKFREELGDNFV
                     ATRGLDDCLFIFPMDEWKNFSEKIASTPVTNKNARFFSRFFISGATESTLDKQGRINI
                     PSNLKEYAGLDKEVVVIGLTNRIELWAKENWDQFLETAEESTDEIASAMEDLGI"
     gene            complement(6224..7399)
                     /locus_tag="VKN64_03605"
     CDS             complement(6224..7399)
                     /locus_tag="VKN64_03605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMA59356.1"
                     /translation="MEQNLNIIHVDMDAFYASVEQLDNPELEGKAVIVGGVGLDNRGV
                     VSTASYEARKFGVHSAMPIAQAKKLCPHGIYLPGRGDRYSELSQQIFEILLKFTPEVE
                     KISIDEAYLNVKGCHRLYGSSAQIAKKIKEAVKNKTGLVCSVGVARNKFLAKIASDLD
                     KPDGLVVIKNDEIDEVLDSLSIKKIPGVGDKTAEKLNEIGIYKIKQLKKLELSELTKL
                     FGKHGKQLFELIRGRDDRKVHTEDKAKSISNETTFHDDIEEMELLLKHLSQLCQKVTR
                     RLRKNKLIGNTVFIKVKDNKFNVRTRRITVQRYIDSTDDLYYLSKQLLEKVKIKNPIR
                     LIGVGIAGLKEKEKEQMSLFAKSENKDEFNLAIDTIKDKFGNRSIRRARDLLEKDNK"
     gene            complement(7417..8439)
                     /locus_tag="VKN64_03610"
     CDS             complement(7417..8439)
                     /locus_tag="VKN64_03610"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HMA59357.1"
                     /translation="MSKLKLGILFGGKSEEHEVSLMSARSVYNNADQTKYEIYPIAVD
                     KKGNWVNKAESRSIIEKDSIEEVPVQTNPIKKGLNNFLKVDLDVIFPLIHGPYGEDGK
                     IQGFFDLLDIPYVGANVLGSAVGMDKGIMKKLFDYNNLRQTEFMTVRKYNDKKYEIIN
                     DFIEKTGLPIFIKPANLGSSIGISKVSSQQDIKEAVEIAFQYDSKIIIEKGIKAREIE
                     CSVLGKNKFKASLPGEIIPSHDFYDYQSKYEDEKTKLIIPADLDDKTIKEVQTTAIKA
                     FEIIEGEGLARVDFFLKDEKVYINEINTIPGFTKYSMYPKLWEASGISYPDLIDDLVN
                     IALEKN"
     gene            complement(8453..9454)
                     /gene="trpS"
                     /locus_tag="VKN64_03615"
     CDS             complement(8453..9454)
                     /gene="trpS"
                     /locus_tag="VKN64_03615"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMA59358.1"
                     /translation="MEKEKRILTGDRPTGKLHLGHYVGSLENRLKLQKEYDTFLIIAD
                     VQALTTNFEEPERLSDDVHQVAEDYLSVGIDPEETTILVQSMIPEIAELTVFYSMIVT
                     VNQLQRNPTIKEESAQYGYDEMTYGFLGYPVSQAADITFCKADLVPVGQDQLPHIEQT
                     RRIVRKFNQLYEPVFKEPEPKISRIPRLVGLDGENKMSKSLDNAIYLNDDEDEVLEKV
                     MSAKTDPARIRATDPGHPEVCTVFQYHKAFNEEDVPEIEERCKAGTIGCVACKKRLAK
                     AINELLEPIRERRQKYLEKPELVDEIIMAGTKHARSIAKETMKEVREAMKLDYFKDK"
     gene            9550..9936
                     /locus_tag="VKN64_03620"
     CDS             9550..9936
                     /locus_tag="VKN64_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMA59359.1"
                     /translation="MAKTRMTKQRKTILEVLKSTDSHPTADWIYEEVKKDIPNISLGT
                     VYRNLNLLAEKGKINVINYANDQSHYDGNTENHYHFRCKKCGNVYDLDLKLFEKEIDK
                     IVDNNTEFEVDTHRLEFYGICPHCRN"
     gene            complement(9941..>10444)
                     /locus_tag="VKN64_03625"
     CDS             complement(9941..>10444)
                     /locus_tag="VKN64_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMA59360.1"
                     /translation="KKMIVTNAAGGLNTTFRPGDLMIVTDHLNFMGSNPLIGENDEEI
                     GPRFPAMTDAHPAHMVDIAEKAADRANIHIMKGVYLAITGPVYETYAMSKFQRLIGAD
                     AVGMSTVPELIAGTHAGLENLAISCITDVVKGKDEESLTHEEVMEVAQKVKPKFKELV
                     NNVIKEL"
BASE COUNT         2994 a         2161 c         1451 g         3838 t
ORIGIN      
        1 tttcaattaa ctctcctttg gcccgggcaa tatcttccac cgaatcaaaa aattcgatat
       61 gagccctccc cacattagtt atcaaaccaa tttgggggtc agatatatcg gccaaaaggc
      121 ttatttcgcc tttgtcgctc attcccatct cgacaataaa aaaatcttca tcaccattta
      181 atcggaataa agtaagggga accccgatat gattgtttaa attcccttca tttcttttta
      241 cattatattt tttatttaaa agattaaata atatatcttt tgtagaagtt ttaccggaac
      301 taccggttat tccaattacc ttaacatctt taaaattggc cctatgaaat ttagccatcg
      361 cctgtagggc ttttgtagta tcatcaactt taattatgtt ctcacattca acctctatat
      421 tctcctgtat caccaaagct tttacttgtt tagccaaatt atcatcaata aagttatgag
      481 catcaaagtt ttcaccttgt aaggcaataa atatatcctc atcatttaat ttccttgtat
      541 ctgtagaaac tcctttaaga atctgttttc tattcccttt tagcaacttg ccttcagtag
      601 cccgaattat ttctttcact ttatatttaa acatctttaa attcactcat tttctctttt
      661 aatgcttttt tagcaaccac tttatcatta aaatcaattg ttttatcagc aaaaacctgg
      721 tatgtttcgt gacccttgcc gataatcact accacatcat cttttcgggc tttgttaatt
      781 gcctcataga tcgcagcttc cctttccact tcaactgtat aaggtgtatc atattgactg
      841 gatttaatac ctttcagaat ttctttaata atgatttcgg gctcttctga acgggggtta
      901 tctgtagtaa caactacata atcacctttc tctacagcaa tttggcccat aatgggtctt
      961 ttccctttat ctctttctcc cccacagccg aatactacga taatatcttt atgttcaaaa
     1021 tgtttgactg cctccaggac atttatcata ctatccggag tatgggcata atcgacaata
     1081 acagtaaaat cttgaccaat atttatggtt tcaaaccgac cgggaatacc ctttacactt
     1141 tctagtgatt ccttgatcaa ctcttctgaa atacccagac tttttgctgc tcccacggca
     1201 gccagactat tataaatatt gaacttacct gttaagttaa gctcatatct taaattatta
     1261 agagtgaatt ttactccctc taaagaaagt tccaaatctt cggcccttaa atcggagacg
     1321 tttttaatgc cataagtaat aatttcattt gtggttgagt ccttaaagaa ctgagagaaa
     1381 tcatcatcat tattaattac agccttacca ttagttttta actttttaaa taatttagct
     1441 ttctcgcctg catattcttc catcgtttca tggtaatcca aatggtcttg ggtaagatta
     1501 gtgaagactc cggctgtaaa gtccatagta tcaactctat gtagggctaa agcatgtgag
     1561 gatacttcca taactgtata cttcacacct ttatcccgca tctcagccag atatctataa
     1621 atatctattg actccggtgt agtacgagaa gcatcaatac ttttctcgcc atcaaaaact
     1681 tcaatcgtac cgattaaacc ggttttatac ccctgatggt ttaatatttc tttgattaaa
     1741 taggtagtgg tggttttgcc gtttgtaccg gttaccccta tcaaatcaat ctcttgtaag
     1801 ggattttcaa agaaattttt agcaagttgg ggcattgcta ttctactatt aggaactttt
     1861 acataagtta tattttcatc ataattatct aaatctttat caattactac cgcattggcc
     1921 tcttttttaa gggcagcatc aatatatttg tggccgtcgg ttttaaaccc ttgtacacaa
     1981 ataaataaat cacccggctc aatattacgg gaatcatatc ttaattcatt aatttctacc
     2041 attttgttgc ctttaatata ttcaacatcc aggttgtcaa taaccttatc taaatacatt
     2101 ataattcacc cttttaattt aagtataacc tctttatcgt catcaattct tagaccagga
     2161 tctacacttt ggccaactac ttgaccggcc tggccctgaa actttaattt aatacccatt
     2221 ttttcagcta gatttaaagc ttcttttttc tccatacctc taaaatcggg agtcattatc
     2281 ctgtaactag ctgctatact atcactcttt tctgtaaaga gaaatactgt tgaattttta
     2341 tatacttccg aacctgcttt aggtatttgg tctattattt cattaccatt acctactatt
     2401 ttatattcca aacctaattc tttcaatgta tttttcacat tatataaatt ttgtcttgaa
     2461 taatccgcta attttaacgt ttcattaacg ggaggttcct tctgtttttc ttgggaaggc
     2521 aacttatagt atctaattag atcattggcg atctttttaa aaagcggagc agccgtctga
     2581 ctggcataat aagtatttcc ctctatcccg tagagtacga ctaaaaccac aaactggggg
     2641 tcattaacgg ggaatattcc aatgaaagaa gaatcatatt tatcttgatt ataatattgg
     2701 gccgtaccag tttttccacc aactctataa ccttctatat aagcattaac cccggttcca
     2761 ttacttacga cattttccag gagattcaat gttttatggg ctgttctact tgaaactacc
     2821 tttctgacct cggtgggaga aagtctcttt tcaactttac ccttttggtt ggtaattcta
     2881 tcaataaaat taggcttcat taataccccg tcatttgcaa cagctgagac tgcggttatt
     2941 agctgaatgg gagttacaga aataccgcgt ccaaaggaga tagtcgccag ttctatatca
     3001 ccgatctgtt cataatcggg aattaagcca atcgcttcac ctggtaattc tatttcggtt
     3061 ttttgaccga aaccaaaagc atctatgtat ttataaaaag aatctttgcc cattctgaga
     3121 ccgacctgga caaagccggg attacaagaa ttttgtacta cctgtgaaaa cagttcttta
     3181 ccatgcccct cttcttccca acattgtata tattctcttt caacttttac cttgccggaa
     3241 tcaaaaaaga catcattttt atttactacc ccttcttcta ttgcagaagc agtagtaata
     3301 attttaaaag tagaaccggg ttcataatta tcggtaattg cccggtttct ccagtatttt
     3361 tgcttatatt gggaaaaatt gttgggatca taagtaggat aattggccat tgatagtata
     3421 ttacctgttt ccggttccat gataataatt gtaccaccgg ccgccttata ctcctcggcc
     3481 gccttggcca attctctttc agaactatgc tggattgctt cattaattgt taaatacagg
     3541 ttactaccat tttcttctgg tatataatca ataatccctt ctgggatcat cttaccagct
     3601 gcatctcttt ctgttaaaat tctacctgca ttaccagtta gatatttatt gtaggttaac
     3661 tcaattcctt ccaatccgtt cccatcaaca cctacaaatc ctaccagctg gctggccaac
     3721 ttatctttag gatagattct tttgctttca tctataaagg taattccatc cagttttaaa
     3781 tctttaatct cttgcacttt ttggtccggt atttttcttt ccaaataaac agcataggca
     3841 tctcttttta acctatttaa taaagtttca taccctatag acaaaatatc ggcaagttgc
     3901 ccggctgttt gagtggggtg ggaaatctgc ttaggcagac ccactaccgt cttgctctta
     3961 atacttacag ccagttcctt gccgttaata tcataaatct ttcctctatc cggattaact
     4021 tttaattctt tcatcctttg ttttaaagcc ttattataat attcctcatt attaataatt
     4081 tgtatccagg ctaatctagc aattaataat aaaaatataa aagtaaaaat gaaaaagaga
     4141 attttaattc ttttttttct atcgttatca accttgttat catccattta gatcatccta
     4201 ttcaggtgag tatgctcgtg cagtacctaa attagtgata aagtcggtaa cttcactaag
     4261 gaaataactc ctttcaggtg tattggctac ttctacttct tctgtcttta gaacaagcgt
     4321 ctgcatagaa tccggttcaa ccatatttaa ttcactgcgg gccaactgat caacctgaga
     4381 aagtgaactt gcattcgaca actttatccc taatttatga tttttttctt ctagagcctg
     4441 tatctcattt tgcatttgat caatacggta acttaaatta ttaattctta aagtataact
     4501 tatataaacc aataatatta tagcacaaat agtcagcaaa attaaatata tatagatatt
     4561 ttttttgtct tcattatttt gttctgcata ttcaccattt tcataatgat tataattatt
     4621 tgtattgtta ttatatcctt ttttattatc tcttttaaac acggctatca cctaccctct
     4681 tcatattttc tcggctactc ttagttttgc acttcttgct ctataatttt cttcaacttc
     4741 ttcttcagtt gctcgaatag gacttttcgt aataattttg accaaaggct ctttatcaca
     4801 aacacaaacc ggaagttcag gaggacatac acaatcactt gccaaatctt taaacttgtg
     4861 tttaactatt ctatcttcaa gtgaatgaaa gctaattata cagatcctcc ctcctttttt
     4921 tagcctattt gtagcacttg aaatcatatc ctctaactgg tttaattcat tatttgtagc
     4981 aattctcagg gcttgaaatg ttctgcgagc aggatgccct cccgaccttc tggctgatgc
     5041 aggaatagct gctttaataa tatcaactaa ttcaaaggtg gtttcaatct cttttttctt
     5101 tctataagct actatgaatt cagcaattct tgctgcccag ttttcttctc cataatcata
     5161 aattatctct tcaagtttat cttttcgata attgttgaca atatctgcgg cagttaattt
     5221 ttgatcccgg ttcatcctca tatcaagggg gccgttgttt ttataactaa aacctctgtc
     5281 agaattgtct aactgagggg aggatacacc gagatcaaaa atcatcccat caacttcatc
     5341 tatctcgtgc ttatctaaaa catcacctat attgatataa ttggcgtgtt ccaatatcaa
     5401 attactgctt tctatactat ccttaactgc ctcaatcgca tctttgtctc tatctaaagc
     5461 tatgactaat cctttttctc ctaagttctc taaaatacca gctgtgtgtc cacctctacc
     5521 taacgttccg tccacataaa ttccatcctt tttaatagac agatattcta ttgtctcttc
     5581 tagtagaact ggtttatgat tattgtgcat ataattggcc tccattagat gcctaaatct
     5641 tccattgcag aagctatctc gtcggtcgac tcttcagcag tttccaagaa ttgatcccaa
     5701 ttttccttag cccataactc aattctattg gtcagaccaa tcacaacaac ttccttatct
     5761 aatccggcat attcttttaa gttggaagga atatttattc taccctgttt atctaaagta
     5821 gattcggtgg ctcccgaaat gaaaaaacgg gagaaaaatc ttgcattttt attagttaca
     5881 ggggttgaag ctattttttc actgaaattt ttccattcat ccatggggaa gataaataga
     5941 cagtcatcta gccctctggt cgcaacaaaa ttatcgccaa gttcttccct aaatttggcg
     6001 gggattatga ctcttccttt actatccatt ttatgtagat attcaccgat taacatattt
     6061 atcaccttaa ccccaatttt accaccatct accactaatc accacttagt atactatttc
     6121 tatgtatata aaaaaaatcc tttaaaataa taaaaaaata ttagtactaa agtactatat
     6181 ttagacaaaa aaatgtgggg ttgcccccac atcggatttt ttcttactta ttatcttttt
     6241 ctaaaaggtc tctcgctctc cttatactcc tgttcccaaa cttatcttta atggtgtcaa
     6301 tcgctaaatt aaattcatct ttattttcac tttttgcaaa taaggacatc tgctcttttt
     6361 ctttttcttt taagcctgct atccccactc caattaatct tatggggttt tttatcttca
     6421 ctttttctaa gagctgtttg cttaaataat aaaggtcatc agtactatct atgtaacgct
     6481 ggacagtaat ccttctagtt cttacattga atttgttatc tttaacttta ataaatactg
     6541 tattgccaat taatttgttt tttcttaatc tccgggtcac tttctgacac agttgagaaa
     6601 gatgttttaa taataattcc atttcttcaa tatcatcatg aaaggtagtt tcgttactta
     6661 ttgattttgc tttgtcctca gtgtgtactt ttctatcatc tcgacctctt atgagttcaa
     6721 aaagctgttt accatgttta ccaaataatt tagttaactc acttaattct aacttcttta
     6781 actgctttat cttatatatc ccaatttcat ttaacttctc agccgtctta tcccccaccc
     6841 ctggtatctt tttaatagat aaagaatcta agacctcatc tatctcatca ttttttatga
     6901 caactaatcc atccggctta tctaaatcag aagctatctt tgctagaaat ttatttctag
     6961 ccactcccac cgaacagaca agtccggttt tgttttttac agcttcttta atttttttag
     7021 ctatttgggc cgaacttcca tataatctat gacaaccttt tacatttaaa taagcttcat
     7081 ctattgatat tttttctacc tcaggggtga attttaataa tatctcaaat atttgctggg
     7141 atagttcaga atatctatct ccccgccccg gtaagtagat accgtgagga cagagtttct
     7201 tagcttgggc aatgggcatc gctgaatgga cgccaaactt tcgggcttca taggaagctg
     7261 ttgatactac gccccggtta tctaagccaa ctcctcccac aataacagct tttccctcta
     7321 attcgggatt atccagttgt tctacagaag cataaaaagc atccatatca acatggatta
     7381 tattcaaatt ttgctccata attaattaaa ccccttttaa tttttctcta aggcaatatt
     7441 aactaaatcg tcgatcaaat cggggtatga tatgccactt gcttcccata gtttgggata
     7501 catgctatat ttcgtaaaac cgggaattgt attaatttca tttatataaa ctttttcatc
     7561 ttttaaaaag aaatctaccc gggccagtcc ctctccttct ataatttcaa aggcttttat
     7621 ggcagttgtc tggacttctt taattgtttt atcatctaaa tcagctggga taatcaattt
     7681 tgttttttca tcttcatact tggattgata atcataaaaa tcatgagaag gtattatttc
     7741 ccccggtaaa gatgctttaa atttattttt acctaaaacc gaacattcta tttctctggc
     7801 ctttatgcct ttttcaataa ttattttact atcatattga aaggcaattt ctaccgcttc
     7861 tttaatatct tgttggctag aaactttaga gatccctata ctggaaccta aattagccgg
     7921 ttttatgaag atggggagac cagttttttc tatgaaatca ttgataattt catatttttt
     7981 atcgttatat tttcttacag tcataaattc agtctgcctt aagttattgt aatcaaataa
     8041 ttttttcatt atccccttgt ccataccaac cgcactacct aatacattag ctcctacata
     8101 ggggatatct aatagatcaa aaaagccctg tatcttacca tcttctccat atggtccatg
     8161 gatcagggga aagataacat ccaaatctac ctttaaaaaa ttatttaaac cttttttaat
     8221 gggatttgtt tggacaggta cttcttctat actgtccttt tctattatac ttctcgattc
     8281 tgccttatta acccaattac cctttttatc aactgcaatt ggataaattt catatttggt
     8341 ttgatcagca ttattataga ctgatctggc tgacatcaaa gatacttcat gttcttctga
     8401 tttacctccg aaaagaatcc ctaattttaa tttactcaaa aaatatcacc tactacttat
     8461 ccttaaaata atctagtttc atagcttctc taacttcttt catggtctct ttggctatac
     8521 ttctggcatg tttggtaccg gccattatta tttcatcaac taattctggt ttttctaaat
     8581 acttttgtct tctttctcta atcggttcta aaagttcatt tattgcttta gcaagacgtt
     8641 tcttgcaagc tacacatcca atagttcccg ccttacacct ttcttcaatt tcaggtacat
     8701 cttcttcgtt aaaggcttta tggtactgaa aaactgtaca cacctcggga tgaccggggt
     8761 cagtcgccct tattcgagca gggtcggtct tagcagacat tactttttcc aatacttcat
     8821 cttcatcatc atttagatat atggcattat ctaatgattt gctcattttg ttttcaccat
     8881 caaggcctac caaccttggt attctactaa tttttggctc cggctcttta aatactggtt
     8941 catataattg gttaaacttc cttacgattc ttctggtctg ttctatatga ggaagttggt
     9001 cctgtcctac cgggacaagg tcagctttac agaatgtaat atcagctgcc tgactaaccg
     9061 gataacctaa aaatccataa gtcatctcat catatccata ttgagccgat tcttctttta
     9121 tagttggatt tctctgtagt tggttaaccg ttacaatcat tgaataaaaa acagttaatt
     9181 ccgctatttc tggtatcata gactggacta atatagttgt ttcttcggga tctattccca
     9241 ctgatagata atcttctgcc acctgatgta catcatctga caatctttca ggctcttcga
     9301 agttagtagt gagagcctga acatcagcta ttatgagaaa agtatcatat tctttttgca
     9361 gctttaatct attttctaaa cttcccacat aatggcctaa atgcaattta cctgttggtc
     9421 tatctcctgt aagtattctc ttttcttttt ccacaattca tcaccttcct gatatcttga
     9481 ctaaatatgt agtatttgat aatatatatt tacgactttg cattactaaa gtaagagagg
     9541 ttatcgaaaa tggctaaaac aagaatgaca aaacaacgta agactatctt agaagtatta
     9601 aagagtacag attcccatcc aaccgctgat tggatttatg aagaggttaa aaaggatatt
     9661 cctaatatta gtttaggaac tgtttatcgg aacttaaatt tactagcaga aaagggcaaa
     9721 ataaatgtta tcaactatgc caatgatcaa agtcattatg atggtaatac tgaaaatcat
     9781 taccatttta ggtgcaaaaa atgtggtaat gtttatgatt tagatctaaa actttttgaa
     9841 aaagaaattg ataaaatagt tgataataat actgaatttg aagttgatac acatcgctta
     9901 gaattttatg gtatctgccc tcactgccgg aattaaatca ttaaagttct ttaatcacat
     9961 tattaacaag ttctttaaac tttggtttaa ctttttgggc aacttccatc acttcttcat
    10021 gtgttaatga ttcttcatct tttcctttta caacatctgt aatacaagat attgctaagt
    10081 tttctaatcc tgcatgggta cctgcaatta gctcaggtac ggttgacatt cccaccgcat
    10141 ctgctccaat taatctttgg aatttactca ttgcataagt ttcataaact ggtcctgtaa
    10201 tagctaaata aacacccttc ataatatgaa tattagccct gtcagctgcc ttttctgcta
    10261 tatccaccat atgagcagga tgtgcatccg tcatagcggg aaagcgcggt cctatttcct
    10321 catcattttc accaataagc ggattagaac ccataaaatt aagatggtct gtcacaatca
    10381 ttaaatctcc gggtctaaag gtggtattca aacctccggc cgcattagta acgatcattt
    10441 tttt
//
LOCUS       DATGCJ010000065         7311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_320848, whole genome shotgun sequence.
ACCESSION   DATGCJ010000065 DATGCJ010000000
VERSION     DATGCJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320848"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1738
                     /locus_tag="VKN64_03630"
     CDS             98..1738
                     /locus_tag="VKN64_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMA59361.1"
                     /translation="MTDRVLTIGIDGGEMSLIDKWIDEGELPNFKKIRENGTSGPLRS
                     TIPPITGAAWSSFQTGTNPGKHGAFNWFKRSEGVYDAEPVTALDIKEPTLWRILNKFE
                     KKVGLIGVPVTTPPEKVENFMIPGLLTTKNASTQSYPADLIDHVKEIAPDFKFSPKEW
                     TRGYIVEDWVDEMVEDIKQKTKLTKQLMKEQDWHFFMVHFMETDQVQHYLWHELDKPP
                     YHIKRVYKAIDNAIGELKEFLDDEDSLLIMSDHGFGELKYNFHIDTWLLHEGYINLKK
                     NFITQFKKLFFKSGFTKEALLPIGEYIYPPLRKLGIVETAVDLASNPMLDWLFISSNN
                     VNWKKSLAYSHSEIGHIFLNIKGRDPEGVIEPEEADQIRDEIIEKLRKFENPFTGKKV
                     TSEIYKGEEVYHGKMTENAPDIVFIPDDMSILGKGAYQFLSNKNVSKSQNQTGHHRMD
                     GIFFAEGPDINQGNEVKGAHIMDIAPTVLYLLDLPLLDSMDGKILTDIIKEEFVEDIE
                     EMYISKSDIDLEKNLRDNLFKDDIEDKKDRLKGLGYVS"
     gene            1752..2714
                     /locus_tag="VKN64_03635"
     CDS             1752..2714
                     /locus_tag="VKN64_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HMA59362.1"
                     /translation="MKILFTFDIKDKYFKSLKESFEGIEIVKSEDIDVLQEEIKDTDI
                     LAAMLMRKFDSDLIEMGNNLKWIQSWSAGVDRFLKEDLFDYLKENDITLTSVRGIHRD
                     SMSEQVLGYLISFSRRLPELLELQKKREWDRLKVDYLKDKTLAIFGLGAVGKEVARKA
                     SVFKMNVIGIKRNPDVNIPHVNNIYGPEQKKEVLDKADYVVVTLPLTDQTRGYFGSEE
                     FAAMQESAYFINVARGEIVKEKELIEALQNETIAGAALDVYQEEPLPKDSPLYELENV
                     ILTPHTSGLFPHYNQEAVKIFKNNLRLFLEDEDLVNVIDPDLQY"
     gene            complement(2689..4329)
                     /locus_tag="VKN64_03640"
     CDS             complement(2689..4329)
                     /locus_tag="VKN64_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA59363.1"
                     /translation="MSERKIKTFLLDGYLDEPSALGVPPYISPHIRYVYGALLSAGLP
                     EEYIDYRTIDQFRTDWDNNIEKLESYDLFIIIAGTTVPGHYLGGRPISLAEIKELGSK
                     VYYPTKVLGGPITLIKDEMDGYDILTRETAALDIYSRLAEMDLDKLRNSAAQYISEWS
                     AKGASLSKLHPSYPNLVIELETFRGCPRENHCAFCSERLKKVHYTRSPQQIIKEVKKL
                     GELGNHHFRLGCQTDILSYAADENGRLNPAKMEELYAGIRKADPHLKVLHLDNINPAT
                     LIHQKEAGHKIIEIITKYNTAGDTAAFGLESADPVVLEKNNIEADPDIAFKAIQLLNE
                     IGGKRVNGVPKLLPGINFLHGLIGERKETFEYNYNFLKKVYEAGLMLRRINIRQVVQI
                     DNYPTEKVDKGKFKEYKKQVNEEINRPMLKRVFPADTILRDVRIEKIEGKISYGRQLG
                     TYPILVAVPGQLEINKFYDVKVIDHGYRSITAFTWPIKISDLSADQLSYIPGIGKKRA
                     SKIFLEEPSSLKDVLNLIEDKETRSLLKKLFNTEGQGQ"
     gene            complement(4354..4695)
                     /locus_tag="VKN64_03645"
     CDS             complement(4354..4695)
                     /locus_tag="VKN64_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59364.1"
                     /translation="MTDRTVKIIRWIARIASVLVIVLGLPFYFGYGNPIPFIEPSYTF
                     MDNLWLTILPLMFIGLGIGWINEKIGGYLVTAPIFIGLLVSFIIQGEMLTLMLFPLIV
                     GMLYLTVYYRK"
     gene            4816..6201
                     /locus_tag="VKN64_03650"
     CDS             4816..6201
                     /locus_tag="VKN64_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMA59365.1"
                     /translation="MSELYNIDQALSLSREEVGENYKNHINPAMVKMLKLLNFDKNYV
                     KADNVEVYDEEGRKFLDFLGGYGSLNLGHNRDEIIEAVNKVTEKPNLLQASLGGIYSA
                     AAHNLAQVTPGDLSISFFGNSGTEAVEGAIKLARAATEKEKIIYCKDSFHGKSYGSLS
                     ITGRDKYQKHFNPLLPATEEVPFGDIKALREKLNSSSNIAAVILEPIQGEGGVIVPPA
                     GYLKEVRDLCNEKEVLMILDEIQTGLGRTGRLFAADHEGVVPDIMCLAKSLGGGIEPV
                     GAYISKPKIWNQAYGSMEKALLHTSTFGGNARASAAVIASINYILTNDLSEQAAEKGE
                     YFLEQLKKFEAEYDLVKEVRGRGLMIGIEFNQPDPGLLDRLSGGLVSKFSAEYMGAMV
                     AGSLNNDYRVITAYTLNNPNVIRLEPPLTVSYEQIDYVVDSLQDIFEKNSNLFNLTMK
                     SAQTAIKNFFN"
     gene            6364..>7311
                     /locus_tag="VKN64_03655"
     CDS             6364..>7311
                     /locus_tag="VKN64_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59366.1"
                     /translation="MQKHSINLVLLVLVFSLLFTPLIEVQASYFTGYLDLDISKKIEP
                     DLSFFIKDNLKEKIFYTVKYDSEPINTYTKIKQGNYLDDSYLVQDLSNQKEYFKVDYK
                     KYNLLYGRYNYQVRTPLFTDYYNHYKGVKFSHQGKVNKLDTFISKTDEISKTDKVLNP
                     NYSVIFLSSRNLKNDSESLYVNILDKDNQLLEIAVLRKGKDYTIDYLNGRISFNPTIF
                     FLNNPEYSYQLIINYKIKGNRREYLKKGYQYSTKIFKKLKLKLYEVSEEEKRNTKGAV
                     IKLIPDSRQRYTLEYQKLKERENNTALSFNNGENYIKDPT"
BASE COUNT         2451 a         1216 c         1448 g         2196 t
ORIGIN      
        1 atataaatct agttattgta ggtatagttt taatgatagt tatttatata agagcgattc
       61 accttagata cagaactgca tagaaaggag atatattatg acagaccgtg ttttgacgat
      121 tggaattgac ggaggagaaa tgtctttaat tgataaatgg atagatgaag gagaactgcc
      181 taactttaaa aagatcagag aaaatggtac ttccggacct ttaagaagta caatacctcc
      241 tatcacagga gcagcctggt cttcttttca aactggcaca aatcccggta agcacggggc
      301 atttaattgg tttaaaagaa gtgaaggtgt atacgatgct gaacctgtaa cagctttaga
      361 tatcaaagaa cccaccttat ggagaatact caataaattt gaaaagaaag tgggcttaat
      421 cggtgttccg gtaaccacac ctccggaaaa ggttgaaaac tttatgattc cgggactttt
      481 gactactaaa aacgcgagta ctcagagtta tcctgctgac ttaatcgatc atgttaagga
      541 aattgcaccg gactttaagt tctctcccaa ggaatggacc cgcggatata tcgtagagga
      601 ttgggtcgat gaaatggttg aggatataaa acagaagacg aagttgacta aacagttgat
      661 gaaagaacag gactggcatt tttttatggt acattttatg gaaacagacc aggtccagca
      721 ttatctatgg catgaactgg ataagcctcc ctaccatatt aaaagggtat ataaagctat
      781 tgataatgcg atcggagaat taaaggaatt tctagatgat gaagactcct tattaatcat
      841 gtcggaccat ggttttggtg agttaaagta taactttcat attgatacct ggcttttgca
      901 tgaaggatat attaatttaa agaagaattt catcactcag tttaaaaaat tattctttaa
      961 atcaggtttt accaaagagg ctttactgcc gattggcgaa tatatttatc ctcccttacg
     1021 taaattaggg atagtagaaa cggccgttga tctggccagt aatcctatgt tggactggct
     1081 ttttatatcc agcaataatg tgaactggaa gaaatctctc gcttattctc atagtgaaat
     1141 aggtcatatc tttttaaata ttaaaggacg tgatccagaa ggagttattg aacctgagga
     1201 agcagatcaa ataagagatg aaataattga aaaattaagg aaatttgaga atccctttac
     1261 cggtaagaaa gtcacttctg agatctataa aggggaagaa gtctaccacg gcaagatgac
     1321 agaaaatgcg cccgatattg tatttatccc agatgacatg tcaatactag gtaaaggtgc
     1381 ctatcagttt ttatccaata agaatgtcag taagtctcaa aaccagaccg gacatcacag
     1441 aatggatgga attttcttcg cagaaggtcc ggatattaat caaggcaatg aggtaaaagg
     1501 ggcccacatc atggatatag cccctacagt actttatcta ttagacttac ctttactgga
     1561 ttcgatggat ggaaagatct taaccgatat aatcaaagaa gaatttgtgg aagatattga
     1621 agaaatgtat ataagcaaat cagatataga tttagaaaag aacctaagag ataacttatt
     1681 taaagatgat atagaggaca agaaggatag actaaaagga ttagggtacg tatcctaggg
     1741 gagtgataga tgtgaaaatt ctttttacct ttgatattaa agataaatat tttaaatcac
     1801 tcaaagaaag ttttgagggt attgaaatag taaagtcaga agatattgat gtactacagg
     1861 aagaaattaa ggataccgat attctggcag cgatgctgat gagaaagttc gacagtgacc
     1921 taattgaaat ggggaataat ttaaagtgga tccagagttg gtctgccggt gtagacagat
     1981 ttttaaaaga agaccttttc gattatttaa aggaaaatga tataacgcta acttcagtgc
     2041 ggggtatcca tagggacagc atgtctgaac aggtcttagg ttatctaatt tctttttcgc
     2101 gccggttacc cgaactactt gaactacaga aaaaaagaga atgggataga ttaaaggttg
     2161 attatcttaa agataaaacc ctggccattt ttggactggg tgcagttggt aaagaggttg
     2221 cccggaaagc aagtgtcttt aagatgaatg taattgggat aaaaagaaat cccgatgtaa
     2281 atattcccca tgtaaataat atttacggcc cagaacaaaa aaaggaagtc ttagacaagg
     2341 cagattatgt agtagttacc ttgcctttaa ccgatcagac ccgcggttac tttggcagtg
     2401 aagagtttgc agccatgcag gaaagcgctt atttcataaa tgtagcccgc ggagagatcg
     2461 taaaagaaaa agagttaatc gaggccttac agaatgaaac catagcggga gcggcccttg
     2521 atgtttacca agaggagcca ctacccaagg attcaccatt atatgaactc gaaaatgtga
     2581 ttttaactcc ccatacttct ggtttgtttc cccactataa tcaggaagcg gttaaaatct
     2641 ttaaaaataa tttgagatta tttttagagg atgaagactt ggtcaatgtt attgaccctg
     2701 accttcagta ttaaaaagtt tttttaaaag actgcgggtt tctttatctt caattaaatt
     2761 taatacatct tttaaagaag agggttcttc taaaaatatt ttagaggccc tcttttttcc
     2821 tataccgggg atataagaaa gctgatcagc tgaaagatca ctgattttaa tcggccaggt
     2881 aaaggctgta attgaacggt agccgtggtc gataaccttt acatcataaa atttatttat
     2941 ttctaattga cccgggacag cgaccaggat aggataggtt cctaactggc gtccgtaact
     3001 tattttgcct tctattttct cgattctgac atctcttaaa atagtatcgg cagggaagac
     3061 cctttttaac atcggcctat tgatctcttc attgacctgc tttttatatt ctttaaactt
     3121 acccttatct actttttcgg ttgggtagtt atcaatttgt acaacctgtc taatattaat
     3181 ccttcttaac attaagcctg cctcataaac cttttttaaa aagttatagt tatattcaaa
     3241 agtttctttt ctttcgccta ttagaccatg taaaaagttg atgcctggta atagtttggg
     3301 cacaccgtta acccgtttac ctccaatttc gtttagaagt tgaatagcct taaaagcgat
     3361 atccggatca gcctcaatat tatttttctc caggacgacc ggatcggccg attctaaacc
     3421 aaaggcggct gtgtcaccgg ccgtattata ttttgtaatt atttctatta ttttatgacc
     3481 cgcctctttc tgatggatta aagtagcagg attaatatta tccaaatgga gaactttaag
     3541 atggggatcg gcttttctaa taccggcata aagttcttcc attttagcag gatttaatct
     3601 accattttcg tcagccgcat aggataagat atcggtctga cagcctaaac gaaaatgatg
     3661 gttacccagt tctcctaatt tttttacttc cttaataatt tgttgaggtg atcttgtata
     3721 atgaactttt tttaatcttt cactacagaa ggcacagtgg ttttcacggg gacagcccct
     3781 aaatgtttcc agttcgataa ccaaattggg ataagaaggg tgtaacttgc ttaagctagc
     3841 tcctttagct gaccactccg aaatatattg ggcagctgaa tttcttaatt tatctaaatc
     3901 catttctgct aaacggctgt agatatctaa agcagccgtt tctcttgtta agatatcata
     3961 accgtccatt tcatctttaa ttagggtgat gggcccgcct aatactttgg ttggataata
     4021 tactttactg cccagttctt ttatttcggc caaagaaatc ggacggccgc ccaagtaatg
     4081 gcctggtaca gttgttccgg cgataatgat aaaaagatcg taggattcca gtttttcaat
     4141 attattatcc cagtccgttc taaattgatc tatggttcta taatcgatat attcttccgg
     4201 gagaccggcc gacagtaaag ccccatatac gtacctgata tgaggggaga tatagggggg
     4261 aacccccagg gcggaaggtt catctaaata accgtccaga agaaatgttt ttatctttct
     4321 ttcactcatc ttaaaaaact ccttaatgca taattatttt ctataataaa ctgttaaata
     4381 cagcattcct acaatcaaag ggaaaagcat caaagtcaac atctctcctt ggattataaa
     4441 actgacgagt aacccgatga aaatgggcgc agtgacaaga tagccgccga ttttctcatt
     4501 tatccagcct atgcccaggc caataaacat gaggggtaaa atagtcagcc atagattatc
     4561 cataaatgta taagagggtt caataaaggg gattggattc ccatagccaa aataaaaggg
     4621 aagacctaat acaataacaa gcactgaagc tattctggct atccatctga ttatcttgac
     4681 agttcggtca gtcattttta aaaaaactcc tttattattt attcctatta taattcatgt
     4741 tataataatt tgtctaatta atagcaggat tttctgtttt aaacataaat atattagata
     4801 gaggaggtca taataatgag cgaattatat aacatagatc aggctttaag tctaagccgc
     4861 gaagaagtag gagaaaacta taagaaccat ataaatccag ctatggtaaa gatgttgaaa
     4921 ttacttaact ttgataaaaa ctatgtaaaa gccgataatg tagaagtata tgatgaagag
     4981 gggagaaagt ttctagattt tttaggaggt tatggttcct taaatttagg acataataga
     5041 gatgaaataa ttgaggcggt taataaggta actgaaaagc ccaatttgct gcaagcttcc
     5101 ttaggtggaa tttatagtgc ggccgcccat aatctagccc aggtaacacc cggcgacctt
     5161 tctataagtt ttttcggcaa cagtggtaca gaagctgttg aaggggcgat caaattagct
     5221 agagctgcca ccgagaaaga aaaaataata tactgcaagg attcctttca cggcaaaagt
     5281 tatggctcac tttcgatcac cggacgtgac aagtatcaaa aacactttaa tcccctcctg
     5341 ccggccacag aagaggtccc ctttggagat ataaaagctt taagagaaaa gcttaacagc
     5401 agcagtaata ttgcagctgt aattttagag cctatccagg gtgaaggagg agtaatagtg
     5461 cctccagccg gatatttaaa agaagtaaga gacttatgta atgaaaaaga agtattaatg
     5521 atcctagatg aaatccagac cggtctgggt agaactggtc ggctctttgc ggccgatcac
     5581 gagggggtcg tgcccgatat catgtgcctg gctaaatcat tgggaggagg tatagaacca
     5641 gttggtgcct atatctcaaa accaaagatt tggaatcaag cctatggcag tatggaaaaa
     5701 gccttactgc atacttccac ctttggaggt aatgcccgcg cttccgctgc tgtaatagct
     5761 tctattaatt atattctaac caatgattta agtgaacagg ccgctgaaaa aggggaatac
     5821 tttttagaac aactaaagaa attcgaggct gaatatgatt tagttaaaga ggttaggggc
     5881 agaggcctga tgattgggat agaatttaac cagcctgatc ccggccttct ggaccgttta
     5941 tcaggtggct tggtcagcaa gttttcagct gaatatatgg gggcgatggt agcaggttct
     6001 cttaacaatg attatagggt gattacggcc tatactttaa acaatcctaa tgtaattaga
     6061 ctggagccgc ccctgactgt gagttatgaa cagattgatt atgtggtcga ctcactacag
     6121 gatatttttg aaaaaaacag taatttattt aacctgacaa tgaaaagtgc ccaaacggca
     6181 ataaagaact tttttaatta atttcagaca aaattcgatg agtttctaca cggcagctga
     6241 ggctgtcgtt ctttttttgc ctttaagtgc taaaaattta caatattaag aaggtattac
     6301 caatcaatat atcaaaggtg ttactgtaca aaaaatattt tatatcgatt aggagtgaat
     6361 ttattgcaaa aacacagtat taatcttgtc ttattggtct tagtcttttc cctattattt
     6421 acacctttga ttgaagtaca ggcttcctat tttacaggtt atctagattt agatattagc
     6481 aaaaagatag agcctgatct ctcatttttc attaaagata atttaaaaga aaagatcttt
     6541 tacaccgtta agtatgatag tgaacctata aatacctata ccaaaattaa gcaaggaaac
     6601 tacttggatg attcatattt agttcaggac ttaagtaacc aaaaagaata ctttaaggtg
     6661 gattataaga agtataactt attatacggc cggtataact atcaggtaag aactcctctt
     6721 tttactgatt attataacca ctataaagga gttaagtttt ctcatcaagg taaagtaaac
     6781 aaattagata cgtttattag taaaaccgat gaaatatcta aaaccgataa agtcttaaat
     6841 cctaattatt ctgttatttt tttaagcagc cgtaatttaa aaaatgattc ggagagcctc
     6901 tatgtaaata ttttagataa ggataatcag ctgctggaaa tagccgtcct gaggaaagga
     6961 aaggattata ctatcgatta tctaaatggg aggatttcat ttaatccaac catatttttc
     7021 ctcaataatc cggaatatag ttatcaactg attattaatt ataaaataaa aggcaataga
     7081 agggagtatt taaagaaagg atatcaatat agtactaaaa tttttaaaaa attaaagtta
     7141 aaattatatg aggtatcaga agaagagaaa agaaatacca agggcgctgt tataaagcta
     7201 atacctgata gcagacaaag atatacatta gaatatcaaa aattaaaaga aagagaaaat
     7261 aatactgcgc ttagtttcaa caatggtgag aattatatta aagacccgac t
//
LOCUS       DATGCJ010000066         7073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_98136, whole genome shotgun sequence.
ACCESSION   DATGCJ010000066 DATGCJ010000000
VERSION     DATGCJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98136"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2934)
                     /locus_tag="VKN64_03660"
     CDS             complement(<1..2934)
                     /locus_tag="VKN64_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMA59367.1"
                     /translation="MIKLFYGKPLNDNNDKISQTITKYMKNNKSENICYIAPTYQMIK
                     DLQSDIFQNNEISTVGPTNFLLFKGLINEILKESTQFQPIINDVQKELILKQVVNNLI
                     DKGEIDYFKNIAKYPGFYEDLLSLIKEISLENEIKEDNFLENIDNLKFQELFKIYNKY
                     YSYLKANDFNDERLQYQKALANIANSSMIDKIDLFIIDGFQYLNLYQKKLINEISELG
                     KDIWLNINYEKNRSGIYNNIDKITAGLKIDKKVKGEANSIENEVFAHLRDNLFSIEYN
                     KIPVDRSLEIVSAEDEESEIRFTARKIKELIYEEEISPDEIGLVVKSQVKYFDLIDEI
                     FSEYKIPYYTSNLKTFKQSGLWILINQLFTLIRNNYDRSSIIKILKSNFTELALGKDE
                     LEKLQLKIWDDGIIRGKDLSYVLNEKSKDYPKPLQAAVKRLINFYKKVRSSNYFKDYS
                     QTILQLLKDLNTVEKIINLNDDDLIVDELKAFKVLESKLKEYEEIITHSLDFEEFVYW
                     FKKIFKEQMIANEDKDNLSRVKVLTPSQARNKSFKYIFIPGLLEGEFPAVNYNKWLVK
                     DKERKILENMGIDLKTKNDLLITESYLFYKSILNSKKKIFLTYPTLGEGEGGAIISSF
                     VEEVRNLFKEDTVHKKIVHNYDFIIEDKDEAFSLQELKDYSVKRLPEAKLKKLIPAFE
                     VESLRNSNQYTNADGLIDAPDILSELNRHFHQDYSYSASALESYVQCPFRFFIEKVLK
                     LEEIKEPEDRLDALQIGDLYHRILFKYFSNNFPGDWKQDLDKYLSGLESSAEKVFAEY
                     EGKRSLSKGVWRVYREEILNNLKLLIEQEYNNDFQTLPYKLEFGFGIAKDYQDSEDNI
                     EDPVEIELIDEKMKLKGKIDRIDSRINQEDLMVYDYKLSDRRGKTKDFFDYNELQLPL
                     YLLALEKMMPDKNLMGGAYYSVRDLNKKGLWKKEFVDYAPKTNRSSTVMDQDNWLRYF
                     ENLKAE"
     gene            3037..3573
                     /locus_tag="VKN64_03665"
     CDS             3037..3573
                     /locus_tag="VKN64_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59368.1"
                     /translation="MRDRFVWTIIKNNWRTLLISLLIIIIGGIIIKNVGEIPYEDQHL
                     MNSTTPIQFNIDGNEMRGFIYLSYWVEAEKNTENMTSYELGEFLQKNGYIELDNYKES
                     LIETLKTYEAEDIKENWMNDYEFFKKRNVYFIEDDEKLMDKMEEIDVNINLKYQLKKI
                     FFVYLDQEKYNEFVDSIE"
     gene            complement(3617..5353)
                     /locus_tag="VKN64_03670"
     CDS             complement(3617..5353)
                     /locus_tag="VKN64_03670"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HMA59369.1"
                     /translation="MDFMQRYKKWLKDDYFDEETKKELEAIKDNEKEIEDRFYKDLEF
                     GTGGMRSKIGAGTNRINKYTIRRATQGFANYIINYYEDEGKNAGAVIAYDPRHKSAEF
                     ALETALVLAANNIKTYLFDSLRPTPELSFAVRELNACGGIVITASHNPPEYNGYKVYG
                     PNGCQLVPDLARKVIAEINEIDNYSMVKTISKEKAIDKDLLEYIGEDIDSKYIEAMID
                     TLPKTELVKEKGQDLNIIYTPLHGSAYKLTERLLKELGFDNLSIVEKQAVFDPDFSTV
                     ESPNPEEYSAFEMALDMAENQDADLIMGTDPDGDRMGAIVKDEKDEYIGLNGNQIGIL
                     MADFLLNNTEVPANGVIIKTIVTTELIRKLAADHGIEVMDTLTGFKYIGELMTAFEEK
                     GNKEFILGFEESYGYLVGDYARDKDAIVATGLTAILALFYKEQGKTLYQRLQEIYEQY
                     GYYKEDLIAIRREGKEGQEKINGTIKNLREKGVKEIADRAVVLWRDYKEGKEFDLKED
                     KTNEIDLPESNVLQYLLEDESLITVRPSGTEPKLKVYFAVNDKTAQAAQQKLEEIRGK
                     FSNRVEEIIENI"
     gene            complement(5711..6796)
                     /locus_tag="VKN64_03675"
     CDS             complement(5711..6796)
                     /locus_tag="VKN64_03675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMA59370.1"
                     /translation="MIPIANPIITEEEKELVNEVMDSGMLIMGEYVKEFEEKFANYSD
                     TEYGIATSSGTTALDVALKSLEIEEGDKIITTPFTFIASSNSILYSKADPIFADVKED
                     TFNIDPDSIRKLLKEHPDTKALLIVHLFGLPCDMDEIMDIVNKHNLLLIEDSAQAHGA
                     TFQGRKVGSFGEVAAFSFYPTKNMTTSEGGIVLTDDEEIMERARLLVNHGSPQKYHHD
                     ILGYNYRMTNISAAIGLIQFKKLEKFNEQRQANAEILTDGLSDLDWLKTPVIPEDRAH
                     VFHQYTIRTDYRNELLEYLKDNEIGYGIHYPMPAYEQSLYQKLGYKDIRCPVAEKLCD
                     QVVSLPVHPALSEEDIDYIIDKLRNFR"
     gene            complement(6824..>7073)
                     /locus_tag="VKN64_03680"
     CDS             complement(6824..>7073)
                     /locus_tag="VKN64_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59371.1"
                     /translation="EKSFIELDYLGRKITRTRRGNGESNKNKQREYEETYENEEEPLK
                     MQLIHFVNSIENKTQPLISGHDGLEALKLTKLIQSKVY"
BASE COUNT         2072 a         1336 c         1053 g         2612 t
ORIGIN      
        1 ttctgccttt aggttttcaa aatatcttaa ccagttatct tgatccatga cagtactgga
       61 acggtttgtc ttgggagcat aatctacaaa ctcctttttc catagcccct ttttatttag
      121 atcacggact gaataatagg cccctcccat taaattcttg tcgggcatca ttttttctaa
      181 ggccaataga taaaggggga gctgtagttc attgtagtca aaaaagtcct ttgtctttcc
      241 ccttctatct gacagcttat aatcatagac catgaggtct tcttgattga tacggctgtc
      301 aattcggtct atcttacctt ttagcttcat cttttcatcg ataagttcaa tctcaaccgg
      361 atcttcaata ttatcttcac tgtcctggta atcctttgca attccaaatc caaactctaa
      421 tttatacggt aaagtctgaa agtcattatt gtattcctgt tcgattaaga gtttaaggtt
      481 atttaatatc tcttccctat atactctcca gacccctttt gaaagagatc gcttaccctc
      541 atattccgca aagacctttt cggccgagct ttctaggccg gataggtact tatctagatc
      601 ttgtttccaa tcacccggaa agttgtttga gaagtattta aaaagtatcc gatggtatag
      661 atctccaatt tgtaaggcat ctaacctgtc ttctggttct ttaatttctt ctaactttaa
      721 caccttttca ataaagaagc ggaagggaca ctgtacataa ctttccaggg cactcgcact
      781 ataactgtaa tcttgatgaa agtgtctatt aagttctgat aaaatatccg gagcatcaat
      841 taaaccgtct gcatttgtat attgatttga atttcttaag ctttcaactt caaaagctgg
      901 aattaatttt tttaactttg cttctggcag ccgcttgaca gaataatctt ttaattcttg
      961 caagctaaaa gcctcatctt tatcttctat tatgaagtca taattatgaa ctatcttttt
     1021 atgaacagta tcttctttaa acaggtttct aacctcttct acaaatgaag aaatgatagc
     1081 gcctccttca ccctctccca gggtgggata ggttaaaaat attttctttt tagagttaag
     1141 gattgattta tagaaaaggt agctttctgt gattaaaaga tcattttttg tttttaaatc
     1201 aatacccatg ttttctaaaa ttttacgttc tttatctttg accagccatt tattataatt
     1261 tacggctgga aattcccctt ctaaaagccc gggtataaag atgtatttaa aactcttatt
     1321 acgggcttga gagggggtaa gcacctttac cctacttaaa ttatccttgt cctcatttgc
     1381 tatcatctgt tctttaaaga tctttttaaa ccagtagaca aactcttcaa aatccaagga
     1441 atgggttatt atctcttcat attcttttaa cttgctttcc agaactttga aggcctttag
     1501 ttcatctacg attaaatcat cgtcgtttaa gttaattatt ttttcaactg tattaagatc
     1561 ttttaataac tgcagtatgg tttggctgta atctttaaaa taatttgaac ttctcacctt
     1621 tttatagaaa ttaattaacc ttttgacggc tgcttgtaat ggcttgggat agtccttact
     1681 tttttcattg aggacatatg ataaatcttt gcccctgata ataccatcat cccatatttt
     1741 aagctgcagt ttttccaatt catctttacc cagtgctagt tcggtaaagt tcgactttaa
     1801 tatctttata atagaacttc ggtcatagtt atttctaatc agggtaaaaa gctgattaat
     1861 taaaatccag agcccgcttt gtttaaaggt ttttaagtta gaagtatagt aaggtatctt
     1921 atactcactg aaaatttcat cgattaagtc aaagtactta acctgacttt ttactaccag
     1981 accgatttca tctggagata tctcctcttc ataaatcagt tccttaattt tacgggctgt
     2041 aaacctgatt tcactctctt catcttctgc cgatacaatc tctaaactcc ggtcaacggg
     2101 tatcttatta tattcaatgg aaaaaaggtt gtctcttagg tgagcaaata cttcattttc
     2161 aattgaattc gcctcacctt tgaccttctt atctattttt aaaccagcag ttattttatc
     2221 tatattatta tagataccac ttctgttttt ctcataatta atatttaacc agatatcttt
     2281 acctaattct gaaatttcat ttatcagttt cttctgatag agattaagat attgaaagcc
     2341 gtcgattata aataaatcta tcttatctat catgctagag tttgctatat tggcaagggc
     2401 tttttgatac tgtagtcttt catcgttaaa atcattagcc ttaagataac tatagtactt
     2461 attatatatc ttaaaaagtt cctggaattt tagattgtct atattttcta agaagttatc
     2521 ttccttaatt tcattttcta aagatatttc tttgatcaaa cttaagagat cttcataaaa
     2581 accgggatat tttgctatgt ttttaaaata atctatttct cccttgtcaa ttaagttgtt
     2641 gactacctgt tttaagatca actctttttg cacatcatta atgattggct gaaactgagt
     2701 actctccttt aatatttcgt ttatcaaacc tttaaataat aggaagttag tgggaccaac
     2761 tgtacttatt tcattgtttt gaaagatatc ggactggaga tcttttatca tctggtaggt
     2821 gggagcaata tagcagatat tttcactttt attgtttttc atatacttag ttatcgtttg
     2881 tgatatctta tcattattat catttaaagg tttaccataa aataatttaa tcataaaatt
     2941 aaacctcact ttttaaatta aataaaggaa tttaaatact taaaaagaat tttattaatg
     3001 aaatatcaaa attttctttt aaaggcgggg attagattga gagacagatt tgtatggact
     3061 attattaaaa ataattggag aacacttctc atttcactac tgattattat aattggcggg
     3121 attatcatta aaaacgtggg agaaatcccc tatgaagatc aacaccttat gaactctacc
     3181 acacctatcc agtttaatat cgatggaaat gagatgcgcg gatttattta tttatcttac
     3241 tgggtagaag ctgaaaaaaa tactgaaaat atgacttcct atgaactagg cgaatttctc
     3301 cagaaaaacg gctacataga actagacaat tacaaagaat cactaattga aaccttaaaa
     3361 acctatgagg ccgaggatat taaagaaaac tggatgaatg attacgaatt ctttaaaaaa
     3421 cgtaatgttt atttcatcga agatgatgaa aagttgatgg acaagatgga agaaattgat
     3481 gttaatatca atttaaagta tcaactcaaa aaaatattct tcgtctatct tgaccaagaa
     3541 aaatataatg aattcgtaga ttcaatagaa taatcttaaa aagaggaggg gcaacccctc
     3601 ctctttatta tttcaactag atattttcta ttatctcttc aaccctgtta gagaattttc
     3661 cccttatctc ctctaacttc tgttgagcgg cctgggctgt tttatcattt acagcaaaat
     3721 atacttttaa ttttggctca gtaccggaag gccggaccgt tatcaggctt tcatcttcta
     3781 ataagtactg taaaacattt gattctggta agtcaatttc attagtttta tcttctttta
     3841 aatcaaattc cttgccttct ttataatctc gccataatac gacggctcta tcagctattt
     3901 cttttacacc tttttctctt agatttttaa tagtaccatt aatcttctcc tgaccctctt
     3961 taccttctct acgtatggct atcaaatctt ctttatagta accatactgc tcatatatct
     4021 cctgtaatct ttggtaaagg gttttaccct gctctttata aaatagcgct aaaatggctg
     4081 ttaaaccggt agcaacgatg gcatctttat ctctagcata gtctcctact aaatagccgt
     4141 agctttcttc gaaacctaag ataaattctt tatttccttt ttcttcaaag gctgtcatca
     4201 gttctccaat gtatttaaat ccagttaagg tatccattac ttctattccg tggtcagctg
     4261 ctaattttct aattaattca gtagtgacta tggttttaat aattactcca ttggcaggta
     4321 cctcggtatt gtttaaaaga aaatctgcca tgagtatacc aatctggtta ccatttaagc
     4381 cgatgtattc atccttttca tcttttacaa tagcacccat ccggtcccca tcgggatcag
     4441 ttcccataat taaatcggca tcttggtttt cagccatatc cagggccatt tcaaaagcag
     4501 aatattcttc cggattggga ctttcaactg ttgagaaatc gggatcaaaa actgcttgtt
     4561 tttctacaat tgagagatta tcaaatccta attccttgag cagtctttcc gttaatttat
     4621 aggcactgcc gtgcagggga gtataaataa tatttaaatc ttgccctttt tctttcacca
     4681 attcggtttt aggcagggta tcgatcatgg cttcaatata tttactatca atatcttctc
     4741 cgatatattc taatagatct ttatctattg ctttttcttt acttatcgtt ttcaccattg
     4801 aatagttatc tatctcattt atctcggcta ttactttacg tgctaaatca ggtactaact
     4861 gacagccgtt tggaccatac accttatatc cattatactc gggtggatta tgactagcgg
     4921 tgatcacaat gcccccgcag gcatttagct ctctaactgc aaaagaaagt tcaggtgtag
     4981 gacgtaaact atcaaataga tatgttttaa tgttatttgc agctagaact aaagcagttt
     5041 ccagggcaaa ctcagctgat ttatggcgtg gatcataagc aataactgcc cccgcatttt
     5101 taccttcatc ttcatagtaa tttattatat agttggcaaa accttgagtg gctctcctga
     5161 ttgtatactt attaatccga ttggtacctg caccaatttt acttctcatt cccccggtac
     5221 caaactcgag atctttataa aaacggtctt caatttcttt ctcgttatct tttattgctt
     5281 ctaattcttt tttggtttct tcatcaaaat aatcatcttt taaccacttt ttatatctct
     5341 gcataaaatc cattataaat tacacctctt tagttggtta atttataccc tcttaatcta
     5401 tactaattca atttttaagt taaaagtcct tttttatttg agcagaaaga gctccctaat
     5461 taggtgggag ctctttaagt atgtgttaat ttaaccagcc tttttaaact tttttaacat
     5521 ttgggagagt tagaagtttt taaaaggctg gttaaatcca aaaatattac atctagataa
     5581 tatacacatt tatagttctc tactaatcaa gaaattcctt cctaaatttg ggtttacatt
     5641 attaaaaagg aaaaccccct catgagaggg agctttatgt actaaattat tgttactatg
     5701 ttggtaaata ttacctaaaa tttcttaatt tgtcgattat ataatctata tcctcttctg
     5761 acaaagcggg atgaacaggc agtgatacga cctgatcaca tagcttctca gcaactggac
     5821 accttatatc cttgtatcct aatttttggt ataggctttg ctcataagcc ggcatcggat
     5881 agtgaatgcc ataacctatt tcattatctt tcagatattc tagtaattca ttacgataat
     5941 cagtcctaat tgtatactga tgaaagacat gtgctcggtc ctcgggaatt acgggtgttt
     6001 taagccagtc taagtcactt agaccgtctg tcagtatttc agcattggcc tgcctctgct
     6061 cattgaactt ctctagtttt ttaaactgaa taaggccaat cgcagctgaa atattagtca
     6121 tccgataatt atatcctaaa atatcatgat ggtatttctg tggtgaacca tgattaacca
     6181 gtaagcgggc tcgttccatg atttcttcat catcggttag aacaataccg ccttcactgg
     6241 ttgtcatatt cttggtcgga taaaaactaa aggctgctac ttcaccaaaa gaaccaacct
     6301 ttctgccttg aaaggtagcc ccatgggcct gagcactatc ctcaataagt agaagattat
     6361 gcttgtttac tatatccatt atttcatcca tatcacaggg aagtccaaat aagtggacta
     6421 ttaataatgc tttagtatcg ggatgttctt tgagcagctt tctgattgag tcgggatcta
     6481 tattgaaagt atcttctttt acatctgcaa agattggatc agcttttgaa taaagaattg
     6541 aattgctgct ggcaataaag gtgaagggag ttgtaataat tttatctcct tcttcaatct
     6601 ctaatgattt tagggctaca tctaaagcag ttgtaccact ggaagttgca ataccatatt
     6661 ctgtatcaga gtaattagca aacttctctt caaactcctt tacatattcc cccattatca
     6721 acataccgct gtccattact tcattaacta gttctttttc ctcttctgtg ataattggat
     6781 tggctattgg tatcatacta aaaatctcct ttcaaaatca attctaatat accttacttt
     6841 gaattaattt agttaatttc aaagcttcta gaccgtcgtg gccgctaatc aaaggctgag
     6901 tcttattttc tatgctgttc acaaagtgaa taagctgcat ttttaatggt tcttcttcat
     6961 tttcatatgt ttcttcatat tctctctgct tattcttgtt agattcgcca ttaccccgcc
     7021 tggttcttgt tatcttccga ccgaggtaat caagttcaat aaatgatttt tca
//
LOCUS       DATGCJ010000067         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_142159, whole genome shotgun sequence.
ACCESSION   DATGCJ010000067 DATGCJ010000000
VERSION     DATGCJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142159"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..486)
                     /locus_tag="VKN64_03685"
     CDS             complement(253..486)
                     /locus_tag="VKN64_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59372.1"
                     /translation="MRDQKAVLRYYLDNGWMVVNQDIFIKLIHENIRDFDILLNDDYQ
                     TSKYYFNIYGELCVIVAGQEFSLSKSTGYDIEF"
     gene            complement(483..1283)
                     /locus_tag="VKN64_03690"
     CDS             complement(483..1283)
                     /locus_tag="VKN64_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA59373.1"
                     /translation="MFKAFYSLSKRPFVKEIETKDLFMSDNFKEVKARLDYLKNTRGI
                     GVVVGEPGSGKTTALRLFADSLNPALFKIIYFPLSTGTVMDFYKGLASGLGEKPKSRK
                     VDLFQQIQQAVTSFYQDQKITPVFILDEMQLAANRFLNDLSILFNFSMDSQNPFLLIM
                     SGLPYFMDKLSLNQNQSLTQRIVMRYYLESLSKEEISDYINHQLKLAGSNHPIFTPQA
                     IEAISLRSRGLPRLVNNLALNSLLMGYQLKADNINEEIVFKACEEAGL"
     gene            complement(1283..2539)
                     /locus_tag="VKN64_03695"
     CDS             complement(1283..2539)
                     /locus_tag="VKN64_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HMA59374.1"
                     /translation="MTDNDREKIALFRFAIISPILNGQVQNQKEYLEEITAKTHNVPY
                     YGPKEYVPKTIQCWLRDYRRGGFNALKPKRRSDYGRSRKVYPELKEKLVKLRKAKPDI
                     SVSLFYDQLIAQKEIKPSDVSYSTIYRIFKREGLLTNTPRKEAHRLRFAHPDVNAMWQ
                     GDFMVGPYLKIGKKKVKTHLFAFIDDCSRIIPYAMFLPTERFSSISKVFSEAILRRGI
                     PQLLYVDNGKIYRSGMLQFACGSLGITLVHTKPYDPASKGKIERVFLTVRKRFIPLLT
                     EKDLTSFENLNECFFDWLEKDYHRKVHSALGITPIEKYMSQIDKVKMVDNPETLKTIF
                     FKRVKRKVKHDRTISLNKMLFEVPPTLIGKRIEVRFNPETFDQVLFYEEGQLIGQAKK
                     VIFADNARIKRKGNISFKNIIKEGDN"
     gene            complement(2696..3208)
                     /locus_tag="VKN64_03700"
     CDS             complement(2696..3208)
                     /locus_tag="VKN64_03700"
                     /inference="COORDINATES: protein motif:HMM:NF040103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HMA59375.1"
                     /translation="MQIIHDFKMRIKDYCNNSMDNDYPVLTDCPVCHDVLIKNGFYKR
                     YVIVSNLSYIIFIRRYRCKHCGLTVSILPSFLLPHFQRSLKDIFNIIYQYLKNRRLLI
                     YRRAVFFYVKRFNKNLNALIIYFRETANGHPCFDNKKAIKLLEMIKECKVPTFAKSFH
                     NHFNKCFMAL"
BASE COUNT         1255 a          590 c          695 g         1160 t
ORIGIN      
        1 ttatagtaaa atagaaatta actttattat tagaatatat atcatattcc tatatagcta
       61 agttataaaa atcattgatc aactaatgta tattgtaaat tagcagatta aaattcccaa
      121 atttatcact ttaaaatagg acaaaaatta ccctgtaaag tgaaaactaa tcaaaataaa
      181 atggtaaaac taagatattt ttcaaaataa attagcaaag aggcatctct aaagttagag
      241 acgccctgag aattagaact caatatcata gccagtggat ttacttaaag aaaattcctg
      301 gcctgcaaca ataacacaga gttcaccata tatattaaaa taatatttgg aggtttgata
      361 atcatcattt aacaatatat caaaatccct gatgttttca tggattaact tgataaatat
      421 atcctggttg accaccatcc agccattatc aaggtaataa cggagaactg ctttttgatc
      481 tctcataagc cggcctcctc acaggcctta aagacgattt cttcattgat gttatcagcc
      541 ttaagctgat aacccatcag aagtgaattt aaggctagat tgttaaccag ccgaggcagt
      601 ccacgggaac gaagtgagat agcttcgatg gcctgaggtg taaaaatggg atgattagaa
      661 ccggctaact taagctgatg gttgatgtaa tcagagattt cttccttgct taaagattca
      721 agatagtatc tcattacgat tctttgggta agggactggt tttgatttaa agaaagctta
      781 tccataaaat aaggcagtcc tgacataatt aatagaaagg gattttgtga atccatagaa
      841 aaattaaata aaatgctgag gtcatttaaa aagcgattag cagccagctg catctcatct
      901 aagataaaga ccggagtgat cttctggtct tgatagaagc ttgttacggc ctgctggatc
      961 tgctggaaga gatcaacctt cctggattta ggcttttcac caagaccgga ggctaaacct
     1021 ttgtagaagt ccataacagt tcctgtagat agtgggaaat agatgatttt aaaaagggct
     1081 gggttaaggg aatcagcaaa aagccttaaa gctgtggttt taccactacc tggttctccc
     1141 acgacgaccc caataccgcg agtgtttttt aaataatcga gtctagcttt aacttcctta
     1201 aaattatcgg acataaaaag atctttggtc tcaatttctt taacaaaagg acgctttgaa
     1261 agcgaataaa aggccttaaa catcagttgt caccttcttt tatgatattt ttaaaagaga
     1321 tattgccctt tcttttaatc cgggcattat cagcaaagat aaccttttta gcctgaccaa
     1381 taagttgtcc ttcttcatag aagagaactt ggtcaaaggt ttcagggtta aaacgaactt
     1441 caatcctttt accgattaaa gttggtggaa cttcaaagag catcttgtta agggaaatag
     1501 ttctatcatg ttttaccttt cttttaaccc ttttaaaaaa gatagttttg agggtttctg
     1561 ggttatcaac cattttcacc ttatcgattt gagacatata cttctcaatt ggagtaatac
     1621 cgagggcgga atgtacttta cggtggtaat ctttttccag ccagtcaaaa aaacactcgt
     1681 ttaggttctc aaaagaagta agatcttttt cagtaagaag gggaataaac ctcttcctga
     1741 cggtaaggaa gaccctttca atcttgccct tgctggccgg atcatagggc ttagtgtgaa
     1801 ctaaggtaat acctagggaa ccacaggcaa actgaagcat acctgagcga taaattttac
     1861 cattatcaac ataaagcagc tgtgggatac ctcttctaag gatagcttcc gaaaacactt
     1921 tgcttatcga ggagaatctt tcggttggta gaaacatagc gtagggaatt attcgggagc
     1981 agtcatcgat aaaagcaaaa agatgggttt taaccttctt tttgccgatc ttaagataag
     2041 ggccgaccat aaaatcgccc tgccacatcg cattgacatc gggatgggca aagcgaagcc
     2101 gatgtgcttc tttacgagga gtattggtca gtagaccttc tcttttgaat atgcgataga
     2161 tagtggagta ggagacatca gaaggtttga tttctttttg agcaatcagt tggtcgtaga
     2221 aaagagaaac tgaaatatcg ggctttgctt ttcttaactt gaccagtttc tccttgagtt
     2281 cgggatagac cttacggctg cggccgtaat cagagcgtct tttaggctta agggcattaa
     2341 atccgccccg gcggtaatca cgaagccagc attggatggt cttgggtaca tattctttag
     2401 gaccgtaata ggggacatta tgagttttgg ctgtgatttc ttctaaatat tccttttggt
     2461 tttgaacctg gccgttaagg ataggagata tgatggcaaa acgaaacagg gctatctttt
     2521 ctctgtcatt atcagtcatt agtttgttcc tcctttttaa gtttagcctt aaagttaaag
     2581 gctaaaaagg gtatattttt gataataaca ttatacttta gaaattaagc cctaatctag
     2641 tgaaagaaaa tgtgttttaa aaaacacttc ccaaataatc acttctatgg taaaattaaa
     2701 gtgccataaa acatttattg aagtgattgt ggaagctctt agcgaaggtg gggaccttgc
     2761 attctttgat catttcaaga agttttatgg cttttttgtt atcaaagcaa ggatgaccat
     2821 tcgcagtttc tctaaaatag atgatgagag cgtttaaatt tttgttaaac cttttgacat
     2881 aaaaaaagac cgctctcctg tagataagga gtcgccggtt tttaagatat tgatagatga
     2941 tattaaagat atcctttaga ctcctctgaa agtgtggcag caaaaaggag ggcaggattg
     3001 aaacggtaag tccgcaatgt ttgcagcggt atcttctgat aaaaattata tagcttaaat
     3061 tagagacaat cacatatctt ttgtagaaac cgtttttaat caaaacatca tggcagacag
     3121 gacagtcagt taaaactgga taatcattat ccatactatt attacagtaa tctttaattc
     3181 tcatcttaaa gtcatggata atctgcatta aaaagcctcc cataccataa taatgtaaaa
     3241 atattttagc acattatttt aggaaaggga agtattaaat acgaaaatat cttgaaaaat
     3301 atatattaaa ataggatatt gtattttgaa agattacagt atatttacaa ctttgtgttg
     3361 atcgaataac cattttgact ccatcttaat agggtggagt tttacgatga actaagtgaa
     3421 taagatggga gtgaaacaag aggcagaaaa taaattttat ttgcgtattt attactaaaa
     3481 aatactaaaa tattacatta ttacaaaata aaagaaaaaa atgaaggatt aacctatttt
     3541 atatagaagt atatatttag aacatatttt aaataaaaaa atatacttat gtttaaaaaa
     3601 atagaaaaat aaggagagga aggttattaa tgaattgcat agatgatttt tattatcttc
     3661 tggtaacatt tataataaat ttatatgaat taactaaaag
//
LOCUS       DATGCJ010000068        13221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_588431, whole genome shotgun sequence.
ACCESSION   DATGCJ010000068 DATGCJ010000000
VERSION     DATGCJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588431"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1731
                     /locus_tag="VKN64_03705"
     CDS             61..1731
                     /locus_tag="VKN64_03705"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA59376.1"
                     /translation="MRSDIEIAQAAEMQPITDIAEKIGLTQDDLELYGDYKAKIKLDT
                     YQRLQSKEDSKLVLVTAITPTPAGEGKTTTTVGLGQALNQKGKDAMIAIREPSLGPTM
                     GVKGGAAGGGYSQVIPMEDINLHFTGDIHAIGIAHNLLSAAIDNHIKQGNELNIDKTK
                     VSWPRVVDMNDRALRDIVLGLGGTGNGEPREDGFIITVASEIMAILCLANDLMELKDK
                     IGNIVIGYSKDDEPITCDDLNVAGAMTALLKDALKPNLVQTLENTPAFIHGGPFANIA
                     HGCNSVMATKMAMKLSDIAVTEAGFGADLGAEKFFNIKSRYADLHPDATVLVATIRAL
                     KMHGGVPKDNLTEENLEALEAGIENLEKHVENINKFNVPLVVAINRFPTDTEAELELV
                     RKKCEEMGVRVALSEVWAKGGEGGLELAEEILDIFENEESNFEFLYSKDLSIKEKIEK
                     IATEVYGADGVDYSNDAASQIETFEKQGYGELPICMAKTQNSISDKPELKGRPTDFRV
                     SVSKVTLSAGAGFIVPSTGPVLKMPGLPKVPSAEDIDIDENGKISGLF"
     regulatory      1782..1882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            1956..2492
                     /locus_tag="VKN64_03710"
     CDS             1956..2492
                     /locus_tag="VKN64_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HMA59377.1"
                     /translation="MKNFSIKKLVYSSFLIALSIILTRLLSLRFAIGGVEGIRIGFGS
                     LPIIFGGIMLGPLAGGIIGAISDLLGYFINPIGAYMPHFTLTSFLTGFIPGIIMYYVF
                     NKNISYFSLLVAIATGQVISSVILVPMFLDMLFGVPFKVTILPKIIGEAVNITVYTYF
                     IKLMSRRNVIKLEIINTN"
     gene            2537..4333
                     /locus_tag="VKN64_03715"
     CDS             2537..4333
                     /locus_tag="VKN64_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HMA59378.1"
                     /translation="MSYQIEVKSNGEQETIECEKGDKLLKVLLQNGYDINAYCGGEGI
                     CGKCKVQLKGDIKEPSSAEIKYLDQKEIDKGFRLSCQYEVESDLEVILEAKEDITVMT
                     GGVITELDIDLDLSKEKISLDKATLKNQTDYLKKIYEKTATNKINYKNLKKIPNLNKK
                     EALTVLKWRDQILNISEDNINENFYGLAVDVGTTTVVIYLLDLNTGKEVDINSFYNPQ
                     KKFGADVISRINYTFNNEDGIEVMQTPVLKAINEGIKTLASNNGITSDDIYKMSIVGN
                     TIMLHLLLGIDALSIAKSPYVPIFTDELDLKPTEVGIEINEHGIIQFLPSISGYVGAD
                     ITGDMLTINIDQLKGNNLMIDIGTNGEIVLNKGERIYSCSAAAGPAFEGANITFGTAG
                     ISGAISTFKIEEKKFKFSTINDNDPIGICGSGLLDMIAEFFKHGFIESSGKIKEKEEL
                     KEWQKEYLDEYKDQRAIKITEKDHSEITIYLTQKDIREVQLAKGAIAAGIEILLNKTE
                     LEADELDRVFIAGGFGNYINPANACFIKMIPQCLEDKIIQIGNGAGTGAKIYLLNKQA
                     KERAVNIKEKVEYIELSVNEEFQTEYINSMSF"
     gene            4348..5031
                     /locus_tag="VKN64_03720"
     CDS             4348..5031
                     /locus_tag="VKN64_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HMA59379.1"
                     /translation="MDTIIKKDIPFKIEFEKFLEKIKLSKDYNDIDTVKKYVKKANEI
                     GKPKAMYREVFINSRGEDYVEVEDQRFESKIMRVNLEDIYKVLIYIVTCGRELEEWAS
                     RQDGDMLEDYWLSMLQEAVLEQARDYIKIEINEKYLPGSSSVMNPGSLIDWSISEQKK
                     LFKLLGNPEKEIGVKLTDSSLMLPKKSVSGLIYPTEKDFENCQVCPRENCPNRSAPYQ
                     PELLKERYE"
     gene            5225..6142
                     /locus_tag="VKN64_03725"
     CDS             5225..6142
                     /locus_tag="VKN64_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMA59380.1"
                     /translation="MSPFSENLRSLRKEANMRQQDLADKLGVAQTTIASYEQGQRFPN
                     HETLIEIADFFNVSIDFLLGREDNKNFDLSKVRKDLEGRLELNEVAQNYFNTLLSGNN
                     QEAEQIIFNALKDDWSLGDVYLNILQPVLFHIGILWETNRIDVFQEHFMSNNIYTILD
                     KLKDRAGNITRENIVKEDKNILLLSVSGEQHQIGLRMINNLLEIKGWNTFYLGINIPS
                     RSVLNAIKNYQADLIMISVTLHEHLNQARNLIQTIRNNKDTRDIKVMVGGAAFRKFSW
                     EDVGADAYALDAKSAVDKVGEIMKESVIK"
     gene            6163..6420
                     /locus_tag="VKN64_03730"
     CDS             6163..6420
                     /locus_tag="VKN64_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HMA59381.1"
                     /translation="MEDKNERYLITFKISSMAFKFENYCKRADLKVKMVPVPSKLSKS
                     CGYACRIKKKDIEDIKELCEDKNIKYSEFYRIDENDQPQKL"
     gene            6637..6933
                     /locus_tag="VKN64_03735"
     CDS             6637..6933
                     /locus_tag="VKN64_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HMA59382.1"
                     /translation="MAILKLDREYMVSSTEQVRNFSKFMDKIKENPIYIMKSNDIEGI
                     VMDIEEYEMLLEKIEYLENKLEDTYIKDKVVTRKQNFNIDDAIDEEEIMKKQED"
     gene            7401..7685
                     /locus_tag="VKN64_03740"
     CDS             7401..7685
                     /locus_tag="VKN64_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HMA59383.1"
                     /translation="MSDDKVRKHIYISGRVQGVGFRANARQKAQAYGIKGWIKNLFDG
                     RVEAVVEGEQDSVQKMVKYLKRGPSFANVTDVEINDEPPTGNFNTFSIKY"
     gene            7699..8301
                     /gene="pncA"
                     /locus_tag="VKN64_03745"
     CDS             7699..8301
                     /gene="pncA"
                     /locus_tag="VKN64_03745"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HMA59384.1"
                     /translation="MNKALLVVDVQNDFYPGGALAVPKANEINSKINSLMESGRYKTI
                     VGTQDWHPKNHRSFASNHNMKPFTEFKAEGLGPVLWPDHCVQGTEGAEFHSDINTDRY
                     NLILRKGYNYKIDSYSAFQENDGTELGLDSYLKGLGIEEVYIVGLALDVCVKFTAQDA
                     VKNGFDTTIILSGSQAIEEEPDKLQEIIDEMKEKGIKFQE"
     gene            8318..8866
                     /locus_tag="VKN64_03750"
     CDS             8318..8866
                     /locus_tag="VKN64_03750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA59385.1"
                     /translation="MARIYGKKIMLREYQEDDLAHMMDWINDPDIVKFLSDRFLYPQS
                     KSQVKDFLEKTMDDSWAGFVISDRETGDYLGQIDFINVDEKNGCAEVALVIGDTNDNS
                     KGIGTEAMQLMMDFGFKNLRLNRIELSCWSYNERALNLYDKLGFTEEGRKRKNRFYDG
                     EYHDEVFFGILREEWQELDDTV"
     gene            8853..9995
                     /locus_tag="VKN64_03755"
     CDS             8853..9995
                     /locus_tag="VKN64_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HMA59386.1"
                     /translation="MTQYNITATTTFGMEGVVKEEVQDLGFKDLAVSNGKVEFKGNEK
                     DICRANLWLRSAERVQIKVGEFEATDFDQLYEKTRDLPWDWFLTKKAHFPVSGKSVKS
                     QLHHVPTCQSIVKKAIVDNLKERYNTEWFSEDGPTYQIEVAMYKDTATLTLDTSGQGL
                     HKRGYRELSSSAPLQETIAAGMIHLSKWDPDRILIDPFCGSGTIPIEAALKAKNIAPG
                     LNREFDFENWHFIKDKDIKEVKDEARARIKKDVEPRLIAGYDKKEDLVSMSRYHAKKA
                     GVSDIIHFQQQDFKDFSTNRNYGYVITNPPYGLRLDNKSKVEKLYKLMGDKLLPLDTW
                     SFYILTAYEDFEKIFGKEASKRRKLYNGGIKVDYYQYYGPWPPRDN"
     gene            10013..10930
                     /locus_tag="VKN64_03760"
     CDS             10013..10930
                     /locus_tag="VKN64_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA59387.1"
                     /translation="MKKFLPYISGLLFSTIFGFSFMFTKEALEVISPFQLMGYRFLTA
                     GLLVNILWLVGILKIDVKFSKIKYLLMVTIAQPVLYFIFETIGMNYTTSSEAGLLVSL
                     IPVVVTILAIFFLNETPSRKQSIFIASSVIGVIFITIMKGTTEVSGNFLGMALIGGAV
                     LMAGAHNILSRKLSVDFKPEVITYFMMTVGALVFNTMLLSTSSTNLSNYFEPLSNIRV
                     LVPVIYLGVLSSVLAFFFVNYTLSKLPASQSAVFANFATVVSILAGVFIRNEPFYWFQ
                     IVGGILIIVGVWGTNYFGKVEEQATELQG"
     gene            10941..11750
                     /gene="dapB"
                     /locus_tag="VKN64_03765"
     CDS             10941..11750
                     /gene="dapB"
                     /locus_tag="VKN64_03765"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMA59388.1"
                     /translation="MSKKRIIVSGAVGRMGKTICKMVIDEDDYELVGAVDVVNQGEDI
                     FNILGIESKKELYITDDLVQEIDDKEAEVVIDFTNPEVVMKNIEKVLQTETNMVVGTT
                     GFDQQDLKQVKEWSNQFNTSIFIAPNFALGAVMMMEFSKKAAKFFDQAEIVELHHDGK
                     MDAPSGTAVTTAEMIAANRSKPKEKVDKKFIEKLEGARGAEFDDIHIHSVRLQGLVAH
                     QEVIFGGKGQTLKIRHDSINRESFMPGIKLAVNKVAELDGLVYGLDKIMDI"
     gene            11778..12803
                     /locus_tag="VKN64_03770"
     CDS             11778..12803
                     /locus_tag="VKN64_03770"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMA59389.1"
                     /translation="MKKYNVAVVGATGAVGTEMLKVLEEYDLPIDNLKVIASPRSKGK
                     EIPFKGEKYTVRTIEKGVFDDIDIALFSIGSSNSNKFVPQAVYEGALVIDNSNAFRMN
                     DDVPLVVPEINPEAIKEHNGIIANPNCSTIQMVVALNPIRKEHVINKIIVSTYQAVSG
                     TGRKAVFELREQSRDYLAGNEVNTDVYSHQIAFNAIPLIDKVNDNGYTEEELKLNRET
                     QKIFANKEIKVTATAVRIPVFYGHSENVHVEFDIPVDLEEIRYLLDNDENIKLMDDPA
                     NDIYPTPIMSENDDSIMVGRLRTDLHNPNSLEMWVVGNNIRKGAALNAVQIADYVIKN
                     NIIDGGE"
     gene            12803..>13221
                     /locus_tag="VKN64_03775"
     CDS             12803..>13221
                     /locus_tag="VKN64_03775"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; diaminopimelate sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMA59390.1"
                     /translation="MSIVVLKFGGSSLKNEKARAAAVDRIEETINKDKKPVVIVSAIG
                     RKGSAYATDTLIKLAEDTFPELAKREKDLIMSCGEIISSVVMVQNIKKKERQARALTG
                     AQAGILTDENYCNSDVININPENILKLINNDIIPVIAG"
BASE COUNT         4638 a         1995 c         2753 g         3835 t
ORIGIN      
        1 tcaatatatt gaaaatatca aagaataaat gatataaaaa ttataaggag tgtgatagtt
       61 ttgagaagtg atattgaaat tgctcaggcg gctgaaatgc agcctattac tgacattgca
      121 gaaaaaatcg gtttaacaca ggatgactta gaactttatg gtgattataa ggccaagatc
      181 aaacttgata cttaccagag acttcagtcc aaagaagaca gcaaattagt attagttacc
      241 gctataactc ctacaccagc cggtgaagga aaaactacaa ccactgttgg tttaggacag
      301 gctttaaatc aaaaaggtaa agatgccatg atagcaatcc gggaaccatc tttaggacca
      361 accatgggtg ttaaaggtgg agctgccggt ggcggctatt cccaggtaat tccaatggaa
      421 gatataaacc tccattttac cggtgatatc cacgcgatag gaatagctca taacctgctt
      481 tcagcagcta tcgataacca tatcaagcag ggcaatgaac taaatattga taagacaaag
      541 gtcagctggc cgagagttgt tgacatgaat gatcgcgccc taagagatat tgtcttgggc
      601 ttaggtggta ccggtaatgg tgaaccgcgc gaagatggct ttatcataac agttgcttcc
      661 gaaataatgg ctatcttatg tctggccaat gacttaatgg aacttaaaga taagatcggg
      721 aatattgtaa ttggttatag taaagatgat gaaccaatta cctgtgatga tctaaatgtg
      781 gcaggcgcaa tgaccgccct acttaaagat gctttaaagc ctaatttagt tcagactctc
      841 gaaaatacac cggcctttat tcacggtgga ccttttgcta acattgcaca cggctgtaac
      901 agtgtgatgg ccactaagat ggctatgaag ttatctgata ttgcagtgac agaagctggt
      961 tttggggcag atctcggggc agagaaattc tttaacataa aatcaagata tgcagactta
     1021 caccccgatg ccactgtatt agttgctact atcagggcgc ttaagatgca cggaggagtg
     1081 cctaaagata acctaaccga agagaatctt gaagctttag aagctggtat cgagaactta
     1141 gaaaaacatg ttgaaaatat taataagttt aatgtaccat tagtggtagc aatcaatagg
     1201 ttcccaactg atacagaagc agaattagaa ttagtgagaa agaaatgtga agagatggga
     1261 gtcagagtcg ccctatctga agtatgggcg aaaggcggcg aaggcggcct tgaattagcg
     1321 gaagaaatcc ttgatatttt tgaaaatgaa gaaagcaact ttgaattcct ttatagtaaa
     1381 gacctttcca tcaaagaaaa gatagagaaa attgctactg aagtctatgg agctgatggt
     1441 gtagattaca gtaatgatgc ggccagtcag atcgagacct ttgaaaagca gggttatgga
     1501 gaactaccga tctgtatggc aaaaacacag aactcaattt cagataagcc ggaactaaaa
     1561 ggtaggccga cggactttag agtatcagta tctaaggtta ccctatcagc cggagcgggc
     1621 tttattgtac catcaaccgg tccagttctt aagatgcccg gtttaccaaa agtaccatcg
     1681 gctgaagata ttgacattga tgaaaatggt aaaatttctg gattgttcta gacaaaaata
     1741 ttaaaatagg aaaaagtaga gagtattaaa aataaatgag tggcagagta gatgccgtgc
     1801 gtatagtgcc ccgggatggg acgttgcctg gggacgaaag ctttccttta aagcgaacgg
     1861 tatggtcatc gcgtccgctg tcaccaaaga agcttctttt tctttggtga ttggttctgc
     1921 catttcactt caaagagaaa ggagcttttt aatttatgaa aaatttctca atcaaaaaat
     1981 tggtatattc ttccttctta atcgcactta gtattattct gacaagactg ctttccttaa
     2041 ggtttgccat cggtggtgta gaagggatta gaatcggatt tggttcccta ccgatcatct
     2101 ttgggggaat tatgttgggc ccattggccg gggggataat aggggctatt tcagatctct
     2161 taggctactt tattaatccc ataggggcat atatgcccca ctttaccctg acctcatttt
     2221 taacgggctt tattccgggg attatcatgt attatgtatt taacaagaat atttcatatt
     2281 tctcattatt ggttgcaatt gccaccggtc aggtaatatc ttctgttata cttgtaccca
     2341 tgtttttgga catgctattc ggggtaccct ttaaagttac tattttgccc aagatcatcg
     2401 gggaagcggt taatattacg gtctatacct acttcataaa attaatgagc aggcggaatg
     2461 tgattaaact ggaaataata aatacgaact gataatatat gtttaatatg gactgaaacc
     2521 agataggggg atagctatgt cataccagat tgaagttaaa agcaatggtg aacaagaaac
     2581 aattgaatgt gaaaaaggcg ataaattatt aaaagtactt ttacaaaacg gatatgatat
     2641 caatgcctac tgtggtgggg aaggcatctg tggtaaatgc aaggtccaat taaaggggga
     2701 tatcaaagaa cccagttcag ctgaaatcaa gtacttagat caaaaagaaa ttgataaagg
     2761 ttttaggtta agctgtcagt atgaggttga gagtgactta gaggttatct tagaagccaa
     2821 agaggatatt actgtaatga ccggaggcgt aattactgaa ctggatattg atctggacct
     2881 tagtaaagaa aagataagtt tagataaagc caccctaaaa aaccaaactg actatctaaa
     2941 aaagatctat gaaaaaacag ccacaaataa gatcaattat aagaacttaa aaaagatacc
     3001 aaatttgaat aaaaaagagg ccttaaccgt tcttaaatgg agagaccaga tccttaatat
     3061 cagtgaagat aatataaatg aaaattttta tggtctagcc gttgatgtgg gtactactac
     3121 tgtagtgatc taccttttag atctaaatac gggtaaagaa gtggatatta actcctttta
     3181 taacccgcaa aagaagttcg gggctgatgt catttcccgt ataaactaca ccttcaataa
     3241 tgaggacggt attgaagtga tgcagacccc tgtactcaag gctatcaatg aaggaattaa
     3301 aaccttagct tccaataatg gtattacttc cgatgatata tataagatga gtatcgtggg
     3361 taatacaatc atgttacacc tcttattggg aattgatgcc ctatccattg ccaagtcccc
     3421 ctatgttcct atctttacag atgaacttga tttaaagcca actgaagtag gaattgagat
     3481 caatgaacat ggtatcatcc agtttttacc ttcaatctcg ggttatgtcg gggctgatat
     3541 aacgggcgat atgttaacca taaatattga tcagttaaaa ggaaataatt tgatgatcga
     3601 tattggtacc aatggagaga tagttttaaa taagggagaa cgcatatatt cctgttctgc
     3661 ggcggctgga ccggcctttg aaggtgctaa tattacattt gggaccgccg gtatttccgg
     3721 ggcgatttcg acctttaaaa tcgaagaaaa aaagtttaaa ttttccacta taaatgacaa
     3781 tgatccgatc ggcatctgtg gctcaggttt acttgacatg atcgctgaat tctttaaaca
     3841 cggttttata gaatcttctg gtaaaataaa agagaaagaa gaattaaaag agtggcaaaa
     3901 agaatattta gatgagtata aagaccagag agcgatcaag ataaccgaaa aagatcattc
     3961 ggaaataact atctatctta cacaaaagga tattagggaa gtacagctgg ctaaaggagc
     4021 aattgcggct ggtattgaaa tacttttaaa taaaaccgaa ttagaggcag atgaacttga
     4081 tagagtcttt atagcaggcg gctttggcaa ttacattaat ccagccaatg cctgttttat
     4141 aaaaatgatc ccgcagtgtt tggaagataa gataatccaa atcggtaatg gggcgggtac
     4201 tggagctaaa atctacttac tgaacaagca agctaaagag agagccgtca atatcaaaga
     4261 aaaagtagag tatattgaac tttccgtcaa tgaagagttt caaactgaat acattaactc
     4321 catgagtttt taaaaaggat gatgataatg gatactataa taaaaaaaga tatacctttt
     4381 aagattgaat tcgaaaaatt tctagaaaag attaaactaa gtaaagacta caatgatatc
     4441 gatacagtca aaaagtatgt caaaaaagcc aatgaaatag gtaaaccaaa ggcgatgtac
     4501 agggaagtat ttattaacag taggggagaa gattatgtag aagtggaaga tcaacgcttt
     4561 gagagtaaaa taatgcgggt taatctagaa gacatctaca aagttttaat ttatattgta
     4621 acctgtggcc gagaattaga ggaatgggcc agccggcagg atggcgatat gctagaagat
     4681 tactggttat ccatgctgca ggaggcggtc ttagaacagg cccgcgacta tataaagata
     4741 gaaattaatg aaaagtactt accgggttct tcctcagtaa tgaatccggg atctttaata
     4801 gattggtcta tcagcgaaca aaaaaagtta tttaaattgc tgggtaaccc cgaaaaagaa
     4861 ataggtgtaa aattaacgga tagttcattg atgctgccta aaaaatcagt ctccgggctg
     4921 atctatccaa cagaaaagga ttttgaaaac tgtcaggtct gcccgcgcga aaattgtccg
     4981 aatcggagcg ctccctatca acctgaactt ttaaaagaaa gatatgaata atttagatat
     5041 ttttattatt aaactctaaa tgctatggag acaataaatc tctatagcat tttttatgat
     5101 aatggaaaaa tataatttat cacaaaaagt gtagtctatt ttacaatttc acaataatga
     5161 tagaaatata ttactatatg tgttataata taataaataa tatttacaat gaggagggaa
     5221 cacaatgtct ccattttcag aaaatttaag aagtttaaga aaagaagcga acatgagaca
     5281 gcaggattta gctgataaat taggtgtagc acaaaccaca attgcaagtt atgaacaggg
     5341 gcagaggttt cctaaccatg aaacattaat agagattgct gattttttta acgtttctat
     5401 agatttttta ttgggtaggg aggataataa aaattttgac ttaagtaaag taaggaaaga
     5461 tttagaaggt aggttagaat taaatgaagt agctcaaaat tacttcaata ctttattatc
     5521 tggtaataat caagaagccg aacaaattat ttttaatgct ttaaaagatg attggtcact
     5581 aggagatgta taccttaata tattacaacc tgtcttattt catataggta tactttggga
     5641 aacaaaccgt attgatgttt tccaggaaca ctttatgtca aataatatct atacaatatt
     5701 agataaactt aaagacaggg ccggtaatat tacaagagaa aatattgtta aagaagataa
     5761 aaatatctta ttattaagtg taagcggtga acagcatcaa attggtttaa ggatgatcaa
     5821 taacctatta gaaattaaag gttggaacac tttctattta ggcattaata ttcccagccg
     5881 cagtgtctta aatgctatta aaaactatca ggccgaccta attatgatct cggtaacctt
     5941 acacgaacat ttaaaccagg cccgtaatct aattcaaact atcagaaata ataaagatac
     6001 aagagatatt aaagtaatgg taggaggagc tgcttttaga aagttcagct gggaagatgt
     6061 aggggctgat gcctatgcac tagatgctaa atcagctgta gataaagtag gagaaataat
     6121 gaaagaatca gtgattaagt aattgacgag gtgataaata tcatggagga taaaaatgaa
     6181 aggtatttaa taacttttaa aatatcttca atggctttta aatttgaaaa ttactgtaaa
     6241 agggctgatt taaaggtaaa aatggtaccg gtgccttcta aattaagtaa atcttgtggt
     6301 tatgcctgcc ggattaaaaa aaaagatata gaagatatca aggaactatg tgaagataaa
     6361 aatatcaaat acagtgaatt ttataggatt gatgagaatg accaacctca aaaattataa
     6421 tttttaatat agttttaatt aatgttctca tattagatct ttcagtcatg aattggaagg
     6481 ttaaatattg aggacatttt tttattttat taaattaaaa aaagttattt agaagtaaaa
     6541 atcataatat tacactttaa aatatgtaaa atagtatgta ttataatatt aagaaaaaaa
     6601 catattacaa tatacgaaat atcggaggtg aatattatgg ctatccttaa attagataga
     6661 gagtacatgg tatcttctac tgagcaagta agaaatttca gtaaatttat ggataaaatt
     6721 aaagaaaacc ctatttatat tatgaaaagc aatgatattg aaggtattgt aatggatatt
     6781 gaagaatatg aaatgttatt agaaaaaatt gagtatttgg aaaacaagtt agaagatacc
     6841 tatataaagg ataaggttgt aactagaaaa caaaatttta atatcgatga cgctatagat
     6901 gaagaagaga taatgaaaaa gcaagaggat taaaattaaa ttactttttc acccagatgt
     6961 aaaagaagat atcgaacaaa tagataatag tattcagcca tatcttaaaa aatctttaaa
     7021 aaagatcaat aaatctccag aattaggtaa accgctggga aatactaata ataaagatct
     7081 ggttggttgt ttaaaaatgt acttctataa taagaaatat ttaaatagta tatgagttaa
     7141 ctaaaagaat cacaaatatt aatttgggca atcggtaaaa gagaagaaga agttgtttat
     7201 ataaatgctt ataaacgtat tttaaagaat ggatttgatt agttttttga tctaatcccc
     7261 attctttttt tattttttaa taaagaattc acattattat taaattgact aaagcctcac
     7321 ctttcttgtt ttagccttat ttatctgtta taattaacaa aaatagtaaa gataaaatgt
     7381 tcattcatgg agggatacac atgagtgatg ataaagttag aaagcatata tacatttcag
     7441 gccgtgtgca aggagttggt tttcgcgcaa atgcaaggca aaaagcacag gcttatggta
     7501 ttaaaggctg gattaagaat ctctttgacg gtagggtaga agctgttgta gaaggagagc
     7561 aagattctgt ccaaaagatg gttaagtacc tcaaaagagg acccagtttt gctaatgtaa
     7621 ccgatgttga aatcaatgat gaacctccaa ctggtaattt taatacattt tcaattaaat
     7681 attaaggggg cttaaaaaat gaataaggcc ttattagttg ttgatgtcca gaatgatttt
     7741 tatcccggtg gtgctctggc tgttcctaag gctaatgaga ttaattctaa gataaattca
     7801 ttgatggaaa gcggtagata taagacaata gtaggaactc aagactggca tcctaaaaac
     7861 cataggagtt ttgccagcaa tcataatatg aagcctttca cagaatttaa ggcggaaggt
     7921 ttaggtcctg ttttatggcc tgaccattgt gtgcagggaa ctgaaggagc tgaattccat
     7981 tcagatataa ataccgatcg atataattta atcttaagaa aaggttataa ctataaaata
     8041 gatagttatt cagcctttca ggaaaacgat ggaactgagc tcggtttgga ttcatattta
     8101 aaaggtttag gtattgaaga ggtctatatt gtcggattgg ctttggatgt atgtgttaaa
     8161 ttcactgcac aagatgcagt taaaaatggt tttgatacaa ctataatctt atctggttcc
     8221 caggcgatcg aagaggaacc agacaaatta caggaaatta tagatgaaat gaaagaaaaa
     8281 ggtattaaat ttcaagaata aaaaatgagg tgattggatg gcacgtatct acggaaagaa
     8341 gataatgtta agagaatatc aggaagatga cttagcccac atgatggact ggattaatga
     8401 tcccgatatt gtaaagttct tatcggaccg ttttctctat ccccaatcta aaagtcaggt
     8461 taaagatttt ttagaaaaaa caatggacga tagctgggcc ggttttgtta tttcagaccg
     8521 tgaaaccggt gactatttag gccagattga ttttattaat gtagatgaga aaaacggctg
     8581 tgctgaagtt gctttagtta ttggtgatac caatgacaac tccaaaggta tcggtactga
     8641 agcaatgcag cttatgatgg actttggctt taagaatttg aggttaaata gaatagaact
     8701 atcctgctgg agctacaatg aaagggctct caatctatat gataaattag gttttaccga
     8761 agaaggcagg aagagaaaaa atagatttta tgatggagaa taccatgatg aagtattttt
     8821 cggtatctta agagaggaat ggcaagaatt agatgacaca gtataatata acagcgacaa
     8881 ccaccttcgg catggaaggt gtagtaaaag aagaagtaca agatttgggt ttcaaagacc
     8941 ttgcagtatc aaatggaaaa gtagaattta aaggcaatga gaaagatatc tgtagagcga
     9001 acctctggct gagatcggcc gaaagagtgc aaattaaagt tggtgagttt gaggccactg
     9061 atttcgacca attatatgaa aaaacaagag atctaccctg ggactggttt ttaacaaaaa
     9121 aagcccattt ccccgtttca ggtaaatcag ttaagtcgca gctgcaccat gtaccaacct
     9181 gtcaatcaat agtaaaaaag gctattgttg ataacttaaa ggaaagatat aatacagaat
     9241 ggttttctga agatggtcct acttaccaga tagaagtggc catgtataag gatactgcta
     9301 ccctgacttt agatacaagt ggtcagggtt tacataaaag aggctatcga gaacttagtt
     9361 cttcggcccc tttacaggag accattgcgg ccggtatgat ccacttaagc aagtgggacc
     9421 ccgaccgaat tttaatagat cccttctgtg gttcgggtac aataccgatc gaagcagctt
     9481 taaaggctaa gaacatagcg cccggcctta acagggagtt tgactttgaa aactggcact
     9541 ttattaagga taaggatata aaagaagtta aagatgaggc cagagccagg attaaaaaag
     9601 atgttgaacc gcgtttaatc gcaggatatg ataaaaagga agacctggtt agtatgagta
     9661 gataccatgc taaaaaagcg ggtgtatctg atatcatcca cttccagcag caggatttta
     9721 aggacttttc gaccaatcga aattatggtt atgtaattac aaatccacct tatggcttga
     9781 gactggataa taagtcgaag gttgagaaat tatataagct aatgggagat aagctgcttc
     9841 ccttagacac ctggtccttt tatatcttga cagcctatga agattttgaa aaaatatttg
     9901 gaaaagaggc ttccaaacgc aggaagttat ataatggagg tattaaagta gactattacc
     9961 agtactatgg tccctggcca cctcgtgata attagacaaa ggagaatatt taatgaagaa
    10021 atttttaccc tacatttccg gactattatt tagtacaatc tttggttttt cttttatgtt
    10081 tacaaaagaa gccttagaag taatttctcc ctttcaattg atgggatacc gttttttaac
    10141 agccggttta ttggtcaata tcctctggct ggtgggaata ttaaagatcg atgttaagtt
    10201 tagtaaaata aaatacctgt taatggtaac aatcgcacaa ccggtcttat actttatctt
    10261 tgaaacaatc ggtatgaact atactacatc ttctgaggca ggacttttag tttcattgat
    10321 acctgtagta gtaactattt tggctatctt tttcttaaac gaaaccccca gccgcaagca
    10381 atctatattt atcgcctcct cggtgatagg tgtcatattt attacgatca tgaaagggac
    10441 cactgaggtt agtggcaact ttctaggtat ggccttaatc gggggagccg ttctaatggc
    10501 gggagctcat aatatattat ctagaaagct ctcggttgat ttcaaaccgg aagttattac
    10561 ttactttatg atgacagtgg gggccttagt atttaacacg atgctgcttt ccacaagttc
    10621 aactaattta tcaaattatt ttgaaccttt aagtaatatc agagtattag tacccgtaat
    10681 ttatttagga gtcttatcat ctgttttggc cttctttttt gttaactaca ctctttctaa
    10741 attacctgct tcccaatcag ctgtctttgc taattttgcc accgttgttt caatcttagc
    10801 aggtgtattt attagaaatg agccgtttta ttggtttcaa attgttggcg gtattttaat
    10861 tatcgtggga gtttggggaa caaactactt tggtaaggtt gaagaacagg caacagaact
    10921 acaagggtga ggtgaaaaag ttgagcaaga aaagaattat tgtcagtggt gctgtcggcc
    10981 gaatgggtaa aacgatctgt aagatggtga ttgacgaaga tgattatgag ctggtcggtg
    11041 ccgttgacgt agtcaatcag ggagaagata tctttaatat tttaggtata gaaagtaaga
    11101 aagaactata tataacggat gacctcgtgc aagaaataga tgataaagag gccgaagtcg
    11161 taattgattt taccaacccc gaggttgtta tgaaaaatat tgaaaaggtc ttacaaaccg
    11221 aaacaaatat ggtagttggt acgaccggct ttgatcaaca agatttaaag caggttaaag
    11281 agtggtctaa ccagtttaat acttccatat ttatagcgcc taactttgct ttaggggccg
    11341 tgatgatgat ggagttttcc aaaaaggccg ctaaattttt tgatcaagct gaaatagtcg
    11401 aactacatca tgatggtaaa atggatgcgc cttctggcac cgctgttaca accgctgaaa
    11461 tgatagcagc taaccgatca aaaccaaaag aaaaggttga taagaagttc attgaaaaat
    11521 tagaaggtgc ccgaggagcc gagttcgatg atattcacat ccacagtgta agattacagg
    11581 gccttgtagc ccatcaggaa gtgattttcg gtggaaaagg tcagacctta aagatcagac
    11641 atgattctat taatagggaa tcctttatgc ccggtattaa attggcggta aataaggtag
    11701 ctgagttaga tggattagta tatgggttag ataaaattat ggatatataa taatgatcat
    11761 aaaggaggag caccctcatg aaaaaatata atgtagctgt agtaggagcc accggagcgg
    11821 tcggcactga gatgctgaaa gttttagaag aatatgatct acctatcgac aatttaaaag
    11881 taatagcttc tccccgttct aagggaaaag agatcccctt taaaggtgaa aagtatactg
    11941 ttcgaaccat agaaaaggga gtatttgatg atattgatat cgcccttttt agtataggta
    12001 gttctaattc caataaattt gtacctcagg ccgtttacga aggggccttg gtgatagata
    12061 acagtaacgc ttttcgaatg aacgatgatg taccattagt tgtaccggag atcaatcctg
    12121 aagctatcaa ggaacacaat ggtattatag caaatccgaa ctgttctacc atccaaatgg
    12181 tggtcgcttt aaatccgatt cgaaaagaac atgttataaa taagataata gttagtacct
    12241 atcaggccgt ttcgggaacc ggtcggaaag cagtttttga actgagagaa caatctagag
    12301 attatctggc cggcaatgag gttaatacag atgtctattc acatcaaatt gccttcaacg
    12361 ccatcccttt aattgacaaa gtaaatgaca acggatatac tgaggaagaa ttaaaattaa
    12421 atcgtgaaac acaaaagatc tttgctaata aagaaatcaa ggtaactgcg accgcagtta
    12481 gaattcctgt tttttacggt cacagcgaaa atgtccatgt agaatttgat atcccggttg
    12541 atttagaaga gatcagatat ctacttgata atgacgaaaa cattaagtta atggacgatc
    12601 cggccaatga tatttatcct actccaatca tgtctgaaaa tgatgatagt attatggtag
    12661 gaaggcttag aaccgattta cacaacccga actcattaga aatgtgggtt gtcggtaata
    12721 atattagaaa aggagcagcc ttaaacgctg ttcaaattgc ggattatgtc ataaaaaata
    12781 atataatcga tggaggagaa taatgtcgat agttgtatta aagttcggtg gttcatcttt
    12841 aaaaaatgaa aaagcaagag ctgctgcagt tgatagaata gaggaaacaa ttaacaagga
    12901 caaaaaaccg gttgtcattg tatctgcaat cggccgaaag ggttctgcct atgcgactga
    12961 tactttaatc aagctggcag aagatacctt tcccgagctt gcaaagcgag aaaaagattt
    13021 gatcatgtcc tgtggtgaga tcatatcttc agttgttatg gttcaaaata ttaagaaaaa
    13081 agaaagacag gcccgtgctt taaccggagc acaagccggt attttgaccg atgaaaatta
    13141 ttgtaattcc gatgtcataa atattaatcc tgaaaatatt ttaaaattaa ttaacaatga
    13201 tattattcca gtgattgccg g
//
LOCUS       DATGCJ010000069         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_321416, whole genome shotgun sequence.
ACCESSION   DATGCJ010000069 DATGCJ010000000
VERSION     DATGCJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321416"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VKN64_03780"
     CDS             complement(<1..383)
                     /locus_tag="VKN64_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA59391.1"
                     /translation="MCQNNYNTASSKGKHLTLDDRKIIAHLYNYQNKSYKEIGEEVNF
                     HRTTISREIDKGKITLKRTQDSYKEVYDYEIAQQVHVNNATAKGPNLKIDKNHEIAQF
                     IEKKVEERYSPQVIAELIKKDDRFE"
     gene            complement(635..>4352)
                     /locus_tag="VKN64_03785"
     CDS             complement(635..>4352)
                     /locus_tag="VKN64_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMA59392.1"
                     /translation="KTDEPEGSKVDIKVKDKNGDVIFEGEPIVDNNGNWSINTETEGQ
                     WTGSSTEFAEGDYEIDATIEDAGGNEAISTGELRVYTSTPKIKAELIGTHNSRKPGEE
                     TSYTLTISNNGKTEVKDMKVTTPLPEGFELDKEAIKINDSILDKDQFEYNGENLIIKR
                     DELKDEATLVIEFSGKIESDGENVLAGTTISTKSKVDYKDILDNVYQEESNNADLEIL
                     KLHSINIKPDRYAAGSPNAQAVFAHKVSNKGNTLEKINLSLQGLEDYEVAWYYDSNEN
                     GTFDQDDELIISSQRKDIKLKALPPEGDSNIILVVNIKEDAVQGEKEFKIKAESSIEN
                     NISDNVNNKLTILDNVPKLNLYDPQNRGAVYTGSNYNLELEYGNTGEDVLSNSKLKIN
                     LSEYVKEAELLNTSEDYSYDSANNTIIVNIDDLQPSEERSISLNITLKDNISQGTEIN
                     TTVDFIQNDRDVIKDSHSIIAANEDPSIIDLSADPETIRGDGQSTSLLTASIKDIMGN
                     FVKDNTEVLFEINNELLSKDNLIKEEGFTTEIEDVGDTDLLRAKVDTKNGLAKINLKA
                     PEISSNEILEIPVNVIAENDDTGRVEETIYIVFSPGVLLGRVIDSDTGLPETGKEVRL
                     IQNNNLIQTTQTDENGRYSFIIEEDGDYEIHVIDKSDESERIKTKKYAEAGNNELTLG
                     PRAIEGRLYYQDTNEPITNTKVELYSPEGKLYGTTKTNDNGQYSFELEEKNTSTSSQN
                     INLQDNNNGKWALKSLDEKVKRGVSDPKAGDIILDADLPVLADNNYGQVLSQKDNETL
                     ENVKVSLINSNTGEKVSLPEINGTNQDNPYYTNAGGEYLFEDVSPGTYYLIAEKEGFY
                     DYQSEEIELSTVSDINNIIKMEPVNDKDLEIEKAADVKYGLPEEEIAYTIRITNNKGR
                     IINELKVTDRLPEELIYIENTANLNGVYEEGEIRWELTDVQPGVTELKYKVKVAKNAE
                     LGKKVVNNVDVNLNGIIINDSHQLQIKPPEILVTKTSNKNQIKIGDFITYQIEVENNT
                     EYDIETFALYDKLPPGFKYIESTASQIKSNSIKTKIKSQGERLIKWDQLSLKANEKLT
                     IRYTLVVGSGVIIGEEYINKAYAKIDGILISNIAQKTVRIKTDSVFNASAIIGKVFFD
                     DNGNGIQDDSEKGLSKVDIISTTGQIITTDQYGRYHLRIASARTNNLGDTVVLKLDKK
                     SLPKNVEIMSNNPVILKISNGMIEKVNFRVKKSK"
BASE COUNT         1190 a          793 c          539 g         1830 t
ORIGIN      
        1 atctcaaaac ggtcgtcctt tttaattaac tctgctatca cttgtggtga atatctttct
       61 tcaactttct tttcaataaa ttgggctatt tcatggtttt tgtcaatctt taaattgggt
      121 cctttagcgg ttgcattatt aacatgtacc tgttgtgcta tctcataatc atatacctct
      181 ttataactgt cttgtgtgcg tttaagagta attttacctt tgtcaatttc tcgacttatg
      241 gtagttctat gaaagtttac ttcttctcca atttctttat agcttttatt ctggtagtta
      301 tataagtgag caattatctt tctatcatct aaagttaagt gttttccttt gctgctagct
      361 gtgttataat tattttgaca catgtcgggg cctccttgtt ttgttttgtg gtgttactca
      421 acattataaa ggaggttacc ggtatgtgtc acatttttat ttgccttacg tgcatttaat
      481 tatacaattc agcatttctt tttaaagtta aaaaccatct aattatattg atgtttttcc
      541 tggttatatt tatctgattt taagaatgat gaaactgagg gggatatccc cctcagttaa
      601 atatcattta tggtgaatag aatatacttt tttattattt agattttttt actctaaagt
      661 ttaccttttc tatcatacca ttcgatattt ttaatattac cggattatta ctcataattt
      721 caacattttt tggtaatgac tttttgtcta actttaaaac tacagtatct cccagattat
      781 ttgttctggc tgaagcaatt cttaagtgat atcttccata ttgatctgta gttattatct
      841 gacctgtagt tgaaattata tctactttag acaagccttt ctcactatca tcctgtattc
      901 catttccatt atcatcgaaa aagactttcc caattattgc tgaagcatta aagacagaat
      961 ctgttttaat tcggacagtt ttttgcgcaa tattagaaat caatatacca tctatcttag
     1021 cataggcttt attgatatat tcttctccaa tgataactcc acttccaacc actaaagtgt
     1081 atctaattgt tagcttttca tttgctttca aactcaattg atcccatttg attaatcgct
     1141 ctccctgaga tttaattttt gtttttatgc tattactttt tatctgagaa gcagtgcttt
     1201 ctatatattt aaatccaggt ggtaatttat cataaagagc aaatgtttct atatcatatt
     1261 ctgtattatt ttcaacttct atttgatagg taataaaatc tcctatttta atttggtttt
     1321 tattagatgt tttggtcact aaaatttccg ggggcttaat ctgaagttga tgactgtcat
     1381 ttataattat tccattcaaa ttaacatcta cattattaac aactttttta cctaactcag
     1441 catttttagc aacctttact ttatatttta attctgtaac gccgggctga acatctgtta
     1501 attcccatct gatttcccct tcttcatata ccccattcaa attggctgta ttttctatat
     1561 atattagctc ttcaggtaat ctatctgtga ccttgagttc gtttattatt cttcccttat
     1621 tatttgttat tcttattgta taagctattt cttcttctgg taatccatat tttacatctg
     1681 ctgctttttc tatttctaag tctttgtcat ttactggttc cattttaatt atattattaa
     1741 tatctgatac tgtacttaac tcaatctctt ctgattggta atcataaaaa ccttcttttt
     1801 cagctattaa atagtaagtg cctggactta catcctcaaa tagatattct cctccagcat
     1861 ttgtataata aggattatcc tgatttgtac catttatttc aggtaaacta accttttctc
     1921 ctgtattact attaattaaa cttaccttta cattttctaa tgtttcatta tctttttggg
     1981 ataaaacctg gccgtaatta ttatctgcta atacgggtaa gtctgcatct aatattatat
     2041 ctcctgcttt aggatctgat acccctcttt ttactttttc atctaatgat tttaatgccc
     2101 atttcccgtt attattatcc tgtaaattta tattttgtga agaggttgaa gtattttttt
     2161 cttccaattc aaatgaatat tgaccattat catttgtttt tgtagtacca tacaatttac
     2221 cttcaggaga atataactca actttggtat tagttatcgg ttcattagta tcttgatagt
     2281 acaatctgcc ttcaattgcc ctaggtccca gagttaattc attattaccc gcctcagcat
     2341 atttctttgt tttaattctc tcactttcat ctgatttatc aattacatga atctcataat
     2401 caccgtcttc ttctattata aagctatagc ggccattttc atctgtttga gttgtttgaa
     2461 ttaaattatt attttgaatt aatctaacct ctttgccagt ttcgggcaat cctgtgtctg
     2521 aatctataac tcttcctaat aagacacctg gagaaaaaac tatataaatt gtctcttcta
     2581 ctctgccagt atcatcgttt tctgctatta cgttaacagg aatctctaag atttcattgg
     2641 aagatatttc aggtgccttt aaattaattt tagctaatcc atttttagta tccacttttg
     2701 ctcttaataa atcagtatct ccaacatctt caatttcagt tgtaaatcct tcttctttaa
     2761 taagattatc tttacttaac agttcattat taatctcaaa taatacttcc gtattatctt
     2821 ttacaaaatt tcccattata tctttaattg aggctgtaag taaagaagta gattgcccat
     2881 ctcccctaat agtttctggg tctgcagaca gatctattat gcttggatct tcattagcag
     2941 caataatact atgactatct ttgattacat ctctatcatt ttgaatgaag tccacagtag
     3001 tatttatttc agttccctga gaaatattat cctttaatgt aatatttaag ctaattgatc
     3061 tttcttcaga aggttgtaga tcatctatat taactataat agtattgtta gccgaatcgt
     3121 aagaataatc ttccgatgta tttagtaatt cggcttcttt gacatattca gatagattta
     3181 tctttaattt tgaattgctt agaacatctt ctcccgtatt accatattcc aattctaaat
     3241 tataattact accggtataa acagctcccc tgttctgagg atcataaagg ttcaatttag
     3301 gtacattatc taaaatagtc agtttattgt taacattatc tgatatattg ttttcaatac
     3361 ttgattcagc tttaatctta aattcttttt ctccttgtac agcatcttct tttatattta
     3421 cgactaagat tatattacta tctccttcag gagggagagc ttttaattta atatcctttc
     3481 tttgagaaga tattataagt tcatcatctt gatcaaaggt gccattctca ttactatcat
     3541 aataccaggc aacttcataa tcttctaatc cctgtaaact taaatttatc ttttctaagg
     3601 tattaccttt attactaact ttatgggcaa atactgcttg tgcattggga ctcccggcag
     3661 catatctatc aggttttata ttgatactgt gtagttttaa tatttctaaa tcagcattat
     3721 tactttcttc ctgataaaca ttatctaaga tgtctttgta atctactttt gatttagttg
     3781 aaatagttgt gccagccaag acattttctc catcagattc aattttgcca gagaattcaa
     3841 taactaaagt tgcttcatct tttaactcgt ctctttttat tattaaattt tcaccattat
     3901 attcaaattg atctttatct aaaatagaat catttatctt tattgcctct ttatcaagtt
     3961 caaaaccttc cggtagagga gttgttactt tcatatcttt aacttcagtt ttaccgttgt
     4021 tactaattgt aagtgtatat gaagtttctt cccctggttt cctgctatta tgggttccga
     4081 ttagttcagc cttgatcttg ggagttgaag tataaactct cagctcacca gtggaaatag
     4141 cttcattgcc accggcatct tcaatagttg catcaatttc ataatctcct tctgcaaatt
     4201 ctgtactgct gccagtccac tgaccttctg tttccgtatt tatactccaa ttaccattat
     4261 tatcaactat aggctctcct tcaaaaataa catctccatt tttatcttta acttttatat
     4321 caactttact accttcaggc tcatctgttt tt
//
LOCUS       DATGCJ010000070         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_499879, whole genome shotgun sequence.
ACCESSION   DATGCJ010000070 DATGCJ010000000
VERSION     DATGCJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499879"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VKN64_03790"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VKN64_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HMA59393.1"
                     /translation="EMVMPGDNIEMNAELITPIAMEEGLRFAIREGGHTVASGVVTEI
                     IE"
     gene            329..478
                     /gene="rpmG"
                     /locus_tag="VKN64_03795"
     CDS             329..478
                     /gene="rpmG"
                     /locus_tag="VKN64_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMA59394.1"
                     /translation="MREKIKLECTECKNRNYTTTKNKQSMRGRLEIKKYCKHCKEHTL
                     HRETK"
     gene            528..728
                     /gene="secE"
                     /locus_tag="VKN64_03800"
     CDS             528..728
                     /gene="secE"
                     /locus_tag="VKN64_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMA59395.1"
                     /translation="MAKLGFLGKVGGFLKKVRLELRKVNWPNREDLQSYTIVVLITVT
                     ALIAFIGVIDVVLTNIITPFIM"
     gene            780..1310
                     /gene="nusG"
                     /locus_tag="VKN64_03805"
     CDS             780..1310
                     /gene="nusG"
                     /locus_tag="VKN64_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMA59396.1"
                     /translation="MEDNTNWYVVHTYSGHEKKVKANLEKRIKTTGMTDKIKEILVPT
                     EEKVRKRGGKKKVVDKKIFPGYIIIHMEMNDETWYVVRNTPGVMGFVSSGTKPLPIEK
                     HEIDHILSGMGLKEEEITIDFEVGDKVMVTDGPFEEFIGIVQEIHPQQQKVKVMVSMF
                     GRETPVELDFDQVEPE"
     gene            1348..1770
                     /gene="rplK"
                     /locus_tag="VKN64_03810"
     CDS             1348..1770
                     /gene="rplK"
                     /locus_tag="VKN64_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMA59397.1"
                     /translation="MAKKVIGQIKLQIPGGQANPAPPVGPALGQHGVNIMDFCKAFNA
                     KTSDKQGTIIPVEITVYADRTFDFITKTPPASILLKQAAGIETASGEPNVDKVATVSK
                     DDVRAIAEQKMKDLNAADIEAAMSMIEGTARSMGILVE"
     gene            1827..2534
                     /gene="rplA"
                     /locus_tag="VKN64_03815"
     CDS             1827..2534
                     /gene="rplA"
                     /locus_tag="VKN64_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMA59398.1"
                     /translation="MAKQSRRYEEALEKVDRNNFYEPQDALELAKNTATANFDESVEF
                     SAKLGVNPKHADQNIRGTLVLPHGTGQEVKIVVFAKGEKAREAEEAGADVVGQEDLAE
                     RIEEGWLDFDLAIATPDMMSVVGPLGRILGPKGLMPNPKAGTVTFDLAESIEEFKAGK
                     LEYRVDSDGNMHLPIGKASFEVDELVDNFKKIMDILMIERPAAAKGKYFRSITVSSTM
                     GPGVKIDPNAALKLIGR"
     misc_feature    2580..2744
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2797..3312
                     /gene="rplJ"
                     /locus_tag="VKN64_03820"
     CDS             2797..3312
                     /gene="rplJ"
                     /locus_tag="VKN64_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMA59399.1"
                     /translation="MARPDKEAAVKELEKEFSKAKSMVVTDYIGLDVAEMTELRAKLR
                     EAGVDYKVVKNTLAKIAANNSDLSEITEFFSGPTAIAFGIEDVVSPAKILDDFAEDHE
                     VLEIKGGYLNGEVISKEKVESLAKIPSREELLAKAFASMKAPITGFVNVLSGNMRNLV
                     SVLSQIKDQKE"
     gene            3336..3704
                     /gene="rplL"
                     /locus_tag="VKN64_03825"
     CDS             3336..3704
                     /gene="rplL"
                     /locus_tag="VKN64_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMA59400.1"
                     /translation="MNKEEIIEAIENMSVLELAELVEELEDKFGVSAAAPVAAAAGGA
                     AGGAEEEEQTEFDVYLEEIGGKKIQVIKAVRELTGLGLKEAKAVVDDAPQNVKEGLSK
                     EEAENMKEALEEAGATVVLK"
BASE COUNT         1500 a          574 c          884 g         1217 t
ORIGIN      
        1 agagatggta atgcctggtg acaacattga aatgaatgca gagttaatca ccccgatcgc
       61 gatggaagaa ggcttaagat ttgcgattcg tgaaggcgga catactgtag cgtctggtgt
      121 tgtaactgaa ataattgagt aggttttaaa cccttaacta aaaaaactag tgtataaaaa
      181 aagcatgtgc gcaatgcaca tgctttttaa aacttgttga cttttacata tatatatgat
      241 aaagtaatta aggctgtaat cacaagaaat taatctattt tatatataaa tacaggaacc
      301 aatattggcc aactgagggg gtggcaccat gcgtgaaaaa attaaattag aatgtacaga
      361 atgtaagaat agaaactata ccaccacgaa aaataaacag agtatgcgtg gtagattaga
      421 aattaaaaaa tactgtaagc actgcaaaga acacacatta catcgtgaga ccaaatagaa
      481 atcaagctta ttaattttta aataactata ataaggacgt gatttaagtg gccaaattag
      541 gttttttggg aaaagtaggt ggctttttaa aaaaagttag acttgaattg agaaaagtta
      601 actggcctaa ccgagaggat ctacaatctt atactatcgt agttttaata acagtcacag
      661 cattaattgc ttttatagga gttattgatg tagttctaac aaatattatt actcccttta
      721 taatgtaatt aaggaggtga ggatcctgta ttcagggtcc ttaaattatg gaagaaaaaa
      781 tggaagataa tactaattgg tatgtagtcc atacctattc aggtcatgaa aagaaagtaa
      841 aggccaattt ggaaaaaaga ataaagacta ccggtatgac agataagatt aaagaaattc
      901 ttgttcccac ggaagaaaag gtaagaaaaa gaggcggcaa gaaaaaagtt gttgataaaa
      961 agatctttcc cggttatatt ataattcaca tggagatgaa tgatgaaacc tggtatgttg
     1021 ttagaaacac cccaggtgtt atgggttttg taagtagtgg aacaaagcct ttacctatag
     1081 aaaaacatga aattgatcat atattatccg gtatgggatt gaaagaagaa gaaattacta
     1141 ttgattttga agttggagat aaagtaatgg tcaccgatgg tccttttgag gaatttatcg
     1201 gtatcgtaca agaaatccat cctcaacagc agaaagttaa agttatggta tcaatgtttg
     1261 gtagagaaac tcctgttgaa cttgattttg atcaagttga accagagtaa tattagattt
     1321 aggagataag aaaggaggaa taatgccatg gcaaaaaaag taataggaca gattaaactg
     1381 cagattcccg gcggacaagc taatccagcc cctcctgtag gacccgcact aggtcaacat
     1441 ggagttaata ttatggattt ctgtaaggct tttaatgcaa agacttctga taagcagggt
     1501 acaattattc ctgttgaaat tactgtatat gcagatagga cttttgactt tattaccaaa
     1561 acaccacctg cttccatttt actaaagcag gctgctggta ttgaaacagc ttcaggtgaa
     1621 cctaatgtag ataaagttgc aactgtaagt aaagacgatg tgagagcaat tgccgaacaa
     1681 aagatgaaag acttgaatgc agctgatatc gaagctgcta tgagcatgat tgaaggtact
     1741 gcccgcagta tgggtatttt agtagagtaa ttcgcgttaa aacagtggga ggtaaaaacc
     1801 gttaatacca caaaggagga gattttatgg caaagcaaag cagaagatat gaggaagctt
     1861 tggaaaaagt ggatagaaat aatttctatg aaccacagga tgcacttgaa ttagcaaaaa
     1921 acacagccac tgcaaatttt gatgaatccg tggaattttc agcgaaatta ggagttaatc
     1981 ctaaacatgc tgatcaaaac attagaggta ctttagtact tccccatgga actggtcaag
     2041 aagttaaaat tgttgtattt gcgaaaggtg aaaaggctcg tgaagctgaa gaagccggcg
     2101 cagatgtagt tggccaggaa gatttagctg aaagaattga agagggttgg ctggatttcg
     2161 atttagctat tgctacacct gatatgatga gtgtagttgg tcctttaggt agaattttag
     2221 gtcccaaagg tttaatgcct aaccctaaag caggcaccgt tacattcgat ttagctgaat
     2281 ctattgaaga atttaaagct ggtaagttag aatatagagt tgacagtgac ggtaatatgc
     2341 atctaccaat cggaaaagct tcttttgaag ttgacgaatt agttgacaac ttcaaaaaga
     2401 tcatggatat tttaatgatc gaaagacctg ctgctgcaaa aggaaaatac tttagatcta
     2461 ttacagtatc ttccaccatg ggtcctggag ttaagattga cccgaatgca gccttgaaat
     2521 taattggtag ataattcatt gacatactta tcaataagtg ttatgcttat tcttggtaat
     2581 aataaaatat agtctaacca tagatagtgg gagtcagcaa tgacttaaac ttttcccgcc
     2641 gaggttatct aagtaaaagc aatgtacatg tatactctac atgtggattg acattacctt
     2701 aaaaatctcc tgcctatcta tggcaggttt ttttatttgt tagactattt taattatcgt
     2761 acagatgggc aaatacaggg aggtgaagtg atatagatgg caagacctga taaagaagca
     2821 gctgtaaaag aattagaaaa ggaattttcg aaagctaaat caatggtagt tactgattat
     2881 attggtttag atgtagcaga gatgacagaa ttaagagcaa agttaagaga agctggagtt
     2941 gattacaaag ttgtaaagaa tactttggct aaaattgcag caaataattc tgatctatcc
     3001 gaaatcactg aatttttcag tggaccgacc gctattgctt ttgggattga agatgtggta
     3061 tctcctgcca agatcttaga tgattttgca gaagaccatg aagtgctcga aattaaaggc
     3121 ggttacttaa atggcgaagt gatcagtaaa gaaaaggttg aatcattagc aaagattcca
     3181 tctagagaag aattacttgc aaaagcattt gctagtatga aggctcccat cactggtttt
     3241 gtaaatgtac ttagtggaaa tatgaggaac ttagtaagtg tattgagtca aattaaggat
     3301 caaaaagaat aaaaattttt taaggaggaa caaagatgaa taaagaagaa attattgaag
     3361 caattgaaaa tatgtctgta ttagaattgg ctgaattagt tgaagaatta gaagacaagt
     3421 ttggtgtaag cgctgctgct cccgttgctg ctgcagctgg tggagccgct ggcggtgcag
     3481 aagaagaaga gcaaactgaa tttgatgttt acttagaaga aattggtggc aagaaaattc
     3541 aggtaatcaa agctgttaga gagttaaccg gtttaggctt aaaagaagct aaagctgttg
     3601 ttgacgacgc acctcaaaat gtcaaagaag gactcagcaa agaagaagca gaaaatatga
     3661 aagaagctct cgaagaagct ggagctactg tagtacttaa gtaagacaaa caaaaagaaa
     3721 gatagtttag atgttactaa aagtgccctt ttattagggc actttttgca gtattaaaat
     3781 atatatttat ttgaccgaaa attacttgac aagatatggg ttctgtgata acattactta
     3841 ttgtcgataa cttttcatat aatggcatat aatatgcata cattttagct tatttttcct
     3901 tatttgatgg atttttatgg acttatttta ttatattatt taataatatt tcattaaatt
     3961 attatacata ctaatttaat tatcaagcat ttaagtaagg tggtgtactt acttaaatgt
     4021 tatatttata aatataagta ttccttgggg gtgcaatgat gtcaacagtg agagagagac
     4081 acagctttgc taaaatcgat gatgcaatgg aattacccaa tttaattcac acacagttaa
     4141 attcatacca gtggttttta gagcaaggct taaaa
//
LOCUS       DATGCJ010000071         7352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_8294, whole genome shotgun sequence.
ACCESSION   DATGCJ010000071 DATGCJ010000000
VERSION     DATGCJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8294"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..2502
                     /gene="recJ"
                     /locus_tag="VKN64_03830"
     CDS             67..2502
                     /gene="recJ"
                     /locus_tag="VKN64_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HMA59401.1"
                     /translation="MNNIWYLEQKENIVHEDKIINQVLLNRGLDTENKVDKFIKAEYS
                     KLADPYLLTDLTKAVQRLIRAINNEERIIIYGDYDVDGITSTSLFIRFFEDINFSNYD
                     YYIPNRLKEGYGLRMDSLDNIDFAKYDLLITVDCGITAVEEVDYLNKQGVDVLITDHH
                     KLGDEIPAAVAVIDPQREESDYFKVLAGVGVVFKFLQAVEREMNLNIIKNHLDLAALG
                     TVADIVELKNDNRIMVKEGLEILKNTKKLGLEKLLSRLKLKGKKINPGQIGYIVAPPI
                     NAIGRMEEPEKGVKLLTTNNKQEAEQISEKLIAINRDRQKKEEEIYKEALAMINKEDI
                     KQQKPIVLHSRDWHRGVIGIVASRLVEKYYLPVILIAIGEDGIGHGSARSISKLDITE
                     GLKYTRDLLENYGGHSMAAGLSIKEDRIDAFKDKLNQYIQEKLSPEDFIPGIKLDSII
                     KSNKINEKFHQKLDLLKPYGVGNPRPKFLLSNIRLKNHYTVGKENKHLKIQLENGISG
                     ICFNMGELDQDIKNKNIDLVAKIYLNNWRGKDEVEIRVEDINVREDHTYFPITFQKNN
                     YTIYDKRGIKRKLKYLEGLKNYRDKIAVFINGSKEIKKLKEELTRAGFEVVINDWRKF
                     NNERADLILFDKVINCKMDKDNHLVLYSIPFSLIDFYKIVKNFDEKIIHLIFNNEDLQ
                     LNERLINQNLPTKKTVKKSQEIISSNFSKDKFPKKEIKLAIKKELNINLNKTAKVLDI
                     LEESKFLKRIDEEINVIGLDNTELDLSNSVYYNNIINIKKRYQNLRRLLRDKNLFKLI
                     NKLTKLEEENDEF"
     gene            2492..3004
                     /locus_tag="VKN64_03835"
     CDS             2492..3004
                     /locus_tag="VKN64_03835"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HMA59402.1"
                     /translation="MNFNDYIRNIPDFPKEGILFKDITPLLKDKDAFTAAIDEMAKEY
                     EDVDIDYVVGIEARGFIIGTPIAYALDKGFIPVRKPGKLPYDKLTASYDLEYGSNELE
                     IHSDAIEEGDNILLVDDLLATGGTTKASVDLIEELGGNIVSIAFLLELVDLNGRKEIE
                     GYDIFTLLQH"
     gene            3042..5186
                     /locus_tag="VKN64_03840"
     CDS             3042..5186
                     /locus_tag="VKN64_03840"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HMA59403.1"
                     /translation="MEIEVLLKKIRSDMDDPDLDIVKNAYEIANEAHEGQYRVSGEPY
                     IEHPINVAYILADLGLDIISISAALLHDVVEDTKFTRDDIEEMFGNEVALLVDGVTKL
                     TKLDFKSKEEHQAESLRKMFLAMAKDIRVVLIKLADRLHNMRTLEHLGREKQIFKSEE
                     TIEIYAPLAHRLGMSRMKSELEDLSFYFLQPDVYDELKKKLKENRSSRNRNIQNAIST
                     LREKIKEQDLNAEIYGRSKHLYSIYQKMQRKEVDFSEIDELTAVRVLVDSVRECYQVL
                     GVVHEIWKPMPGRFKDYIAMPKSNMYQSLHTTVIGPSGDPLEVQIRTHEMHKTAEYGI
                     AAHWRYKEGKTDNDDYEEKLSWLRQLLEWQKDLQEPQEFMKALKIDLFQDEVFVFTPK
                     GDVMSLPRGGTPIDFAYHIHTEVGHNCVGAKVNGKIVPLEYELNNGDIVNIITSKKST
                     GPSRDWLKYVKTSTARSKIKRWFKKQQRDEIIRRGRELLNREFDRQHIDLSSKEKEKE
                     LERIASELGENNKENLMESIGYNNISVKQVINKLDIEEEEEVTLEELQTAPSTKSSDK
                     GVKVKGVDNLKVRTAKCCNPVPGDKIVGYITRGRGISIHRKGCPNLKSLIENEEERII
                     DVEWDQQRSESYVVDLNIEAVDKSALLNDITRLIKNEQIKLLSVNARADKYGKAYIKL
                     SVELSSIEHMKDIMNKIKDISGVLSVQRAKPT"
     gene            5197..5655
                     /gene="dtd"
                     /locus_tag="VKN64_03845"
     CDS             5197..5655
                     /gene="dtd"
                     /locus_tag="VKN64_03845"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMA59404.1"
                     /translation="MIQLRAVVQRVKESKVKVEDEVIGSIEEGLLIFLGIGNDDSMED
                     IDYLIDKIVNLRIFEDMEGKMNLSALDLEKEIMVVSQFTLYGDCRKGRRPSFFDAAPP
                     DKAERLYDVFVSKIKDYEINIETGEFQAMMDVDLVNDGPVTLLIDSKKNF"
     gene            5670..6299
                     /locus_tag="VKN64_03850"
     CDS             5670..6299
                     /locus_tag="VKN64_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA59405.1"
                     /translation="MIISQTVGVNFTNCYLIGKKNSKAIVIDPGDEIDKIMALVEKNN
                     VEVEKIINTHGHFDHISANNELKEKTNAEVYIHEKDKDALVNPEKNLSTHLGQKNLVE
                     LKEADHILREGDTVQIEDYSFEVLHTPGHSPGSISLYDSNKKILISGDTIFSMGVGRT
                     DFPGCSQEELINSIENKLLTLPDETKVYPGHGQETTIGKFKVKVWERLT"
     gene            6411..>7352
                     /locus_tag="VKN64_03855"
     CDS             6411..>7352
                     /locus_tag="VKN64_03855"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HMA59406.1"
                     /translation="MFDNLRNNSKIIVYIVVAAFVVTGGLMGFGSYMSRNNPSGSNRS
                     SQYIATVNDKGITPQQFLSVLRNSASQANNLSQGEVIPFRLNVLKSMIEREILLSEAE
                     KQDINVEISDGEVEERIDEILNQNDLTQEELKDNLESQDYTYEQFKTDIRNSLRNSER
                     ITKVKETTYSNIEVTEEEIKETYEERYADSEEKPELEEVRSDIKESLLASKQNEAYQN
                     WLENKKSEANVTINDPVLYAYNALDQGNYQVAIDSFQNMVNSENSNTSASIYTYLAQA
                     YAGAEQYDKALETFNTALEDYPENWELRLNFGEYYANR"
BASE COUNT         2827 a          962 c         1464 g         2099 t
ORIGIN      
        1 atctaaggta tatgctaccc ccttataaaa tagggggttt tgtatttaaa atgataggtg
       61 ataactttga ataatatctg gtatttagaa caaaaagaaa atatagtgca tgaagataag
      121 ataattaatc aggttctttt aaatagaggg ctggatactg aaaataaggt tgataaattt
      181 attaaggctg aatacagtaa acttgcggac ccctatcttt taacagactt aacaaaagcg
      241 gtgcaaagat taataagagc tataaataat gaagaaagaa ttattatata cggtgattat
      301 gacgtagatg gaatcacctc aacttcacta ttcattagat tttttgagga tataaatttt
      361 tctaattatg attactatat tcccaaccgt ttgaaagaag gttatggttt acgaatggat
      421 agtttagata atattgactt tgcaaaatat gatctattaa taaccgtaga ctgcggcata
      481 acagctgttg aagaagttga ttatctaaat aagcagggtg tagatgtttt aatcacagat
      541 caccataagt taggcgatga aattcccgca gccgttgctg taattgaccc gcagagagaa
      601 gaatctgatt attttaaagt actggccgga gtcggagtag tatttaagtt tttacaggca
      661 gtagaacggg aaatgaacct aaatattatt aaaaaccatc ttgatttagc agcattaggg
      721 actgtggccg atattgtaga actaaaaaat gataacagga taatggttaa agaaggttta
      781 gaaatcttaa aaaacaccaa aaaattaggt ttagaaaaat tattatccag gctcaaacta
      841 aaagggaaga agataaatcc cggtcaaata ggttatatag ttgctcctcc cataaatgcg
      901 attgggagaa tggaagaacc ggaaaaaggt gttaaattat taactactaa taataagcaa
      961 gaagcagagc aaataagtga aaaattaatt gctatcaacc gcgatcggca aaaaaaagaa
     1021 gaagaaatct ataaagaagc tctggctatg attaataaag aagatataaa acagcagaaa
     1081 ccgattgtac tacattctcg tgattggcat cgaggagtaa tcgggattgt ggcctcaaga
     1141 ttggttgaaa aatattatct acctgttatt ttaattgcga ttggggaaga tggtattggt
     1201 cacggttcag cccgaagtat tagtaagctg gatataactg aaggcctcaa gtataccaga
     1261 gaccttctag aaaattacgg cggccattcc atggcggcag gattatcgat taaggaagat
     1321 aggatcgatg catttaagga taaattaaat caatatatac aggaaaaatt gagccccgag
     1381 gattttattc ctggaatcaa gcttgattca attattaaaa gtaataagat taacgaaaaa
     1441 tttcatcaaa aacttgatct tttaaaaccc tatggggttg ggaaccctcg tcccaaattt
     1501 ttactaagta atattagatt aaaaaaccat tataccgtag gtaaagaaaa taaacacctg
     1561 aaaattcaat tggaaaatgg catttcaggc atttgtttta atatgggaga gttagatcaa
     1621 gatataaaaa ataaaaatat tgatttagta gcaaaaattt atttgaataa ctggcggggc
     1681 aaagatgaag tggaaatcag agtagaagat attaatgtta gagaagacca tacctatttt
     1741 cctattactt ttcaaaaaaa taattacacg atctatgata aaaggggtat taaacgaaag
     1801 cttaaatatc ttgaaggatt gaaaaattat cgggataaga ttgcagtttt tattaatggg
     1861 agtaaagaaa tcaaaaaatt aaaggaagaa ctaacaagag cgggatttga ggttgtaata
     1921 aatgattgga gaaaatttaa taatgagcgg gcagatctca tcctatttga taaggttatc
     1981 aactgcaaaa tggataaaga taatcattta gtcctgtatt caatcccatt ttcacttatt
     2041 gatttctata aaatagttaa gaactttgat gaaaaaatta ttcatttaat attcaataat
     2101 gaggatttac agttaaatga aaggctaatt aaccaaaatt taccaaccaa aaagacagta
     2161 aaaaaatcac aagaaataat tagctccaat ttttccaaag ataaatttcc aaaaaaagaa
     2221 attaaattag caataaaaaa agaattgaac ataaatttaa ataaaacagc caaagtatta
     2281 gatatattag aagaaagtaa atttttaaaa agaattgatg aagagattaa tgttataggt
     2341 ttagataata ctgaattgga cttatcaaat tcagtatatt ataataatat tattaacatt
     2401 aagaaaaggt atcaaaattt gagaagatta ttaagggata agaatctgtt taaattaatt
     2461 aataagctta caaaactgga ggaggagaac gatgaatttt aatgactata ttagaaacat
     2521 acccgatttt cctaaggaag ggatattgtt taaagatatt accccactac tcaaagacaa
     2581 agatgcattc acagctgcaa tagatgaaat ggcaaaagag tatgaagatg ttgatattga
     2641 ttatgtggtt ggtattgaag cccggggctt tattattggt acccctattg cttatgcttt
     2701 agataaagga tttattccgg taagaaaacc aggtaaacta ccatatgata aattgactgc
     2761 cagttatgat ctggagtacg gaagtaatga acttgagata catagtgatg caatagaaga
     2821 aggagacaac atcttattag tagatgacct cttagctaca ggtggaacaa ctaaagcatc
     2881 agttgatttg attgaagaat taggtggaaa tatagtcagt attgcttttc ttttagaatt
     2941 agtagatctc aatggaagaa aagagattga agggtatgat atattcacac tcctacagca
     3001 ctaactttag actaaacttt ttatgaaatg aagggattaa tatggaaata gaggttttat
     3061 taaaaaagat aaggtctgat atggatgacc ccgatcttga tattgtaaaa aatgcatatg
     3121 aaattgctaa tgaagctcat gaaggtcaat acagagtttc aggtgaacca tatatagaac
     3181 accccatcaa tgtggcatat atattagctg accttggttt ggatataatc tctatttctg
     3241 ctgccctact gcatgatgta gtagaggaca ctaaatttac tagagatgat attgaagaga
     3301 tgttcggtaa tgaggttgct cttcttgtgg atggggtaac taaattaacc aaattagatt
     3361 tcaagtctaa agaagaacat caggcggaaa gtttaaggaa gatgtttctg gcaatggcca
     3421 aagatatcag agttgtttta attaaactag ctgacaggct tcataatatg aggacgcttg
     3481 aacatttagg ccgggaaaaa caaatcttta aatccgaaga aacaattgag atttatgccc
     3541 ctttagctca tcgtttaggg atgtccagaa tgaagtctga attagaagat ttaagctttt
     3601 attttcttca gcctgatgta tatgatgaat tgaaaaagaa actaaaagaa aataggtcat
     3661 cccgaaatag aaatattcaa aatgctatca gcactctacg tgagaagata aaagagcagg
     3721 atttaaatgc cgagatatat ggtcgttcca agcatcttta cagtatctat caaaaaatgc
     3781 agcgtaaaga ggtagatttt agtgaaatag atgagctaac agctgttagg gttcttgtag
     3841 atagcgttag agaatgttat caggtattag gtgtggttca tgagatatgg aaaccaatgc
     3901 cgggccggtt taaagattat atagcaatgc ctaaatcgaa tatgtatcaa tccttacaca
     3961 ctaccgtaat tggccccagt ggtgacccgc tggaagtaca aatcaggact catgagatgc
     4021 ataaaacggc cgaatatggt attgctgccc actggcgata taaagaaggt aaaaccgata
     4081 acgatgacta tgaagagaag ttatcctggc tcagacagtt gctggaatgg caaaaagact
     4141 tacaggaacc acaggagttt atgaaggcct taaagattga cttattccag gatgaggtat
     4201 ttgtctttac tccgaaaggt gatgtgatgt cattaccgcg aggaggaact cctatagatt
     4261 ttgcctacca tatccatact gaagttggtc ataactgtgt gggagccaaa gtaaatggca
     4321 agatagtacc tttagaatat gaattaaaca acggagatat tgtaaatata attacttcca
     4381 aaaagagcac cggtcccagt cgagactggc ttaaatatgt taaaacttct accgcccgca
     4441 gtaagattaa aagatggttt aaaaagcagc agagagatga aataattagg cggggaagag
     4501 aactattaaa tagggaattt gatcgccagc atatcgatct ttcaagtaaa gaaaaggaaa
     4561 aagagctaga aagaatcgca tccgagttag gtgaaaataa taaagaaaac ctaatggagt
     4621 caataggtta taataacatc tccgtcaagc aggtaataaa taaattagat attgaagagg
     4681 aagaagaagt tacactagaa gaactgcaaa ctgctccttc tactaaaagc tccgataaag
     4741 gagtcaaggt taaaggtgta gacaatttaa aagttagaac agctaaatgt tgtaacccag
     4801 tacccgggga taaaatcgtg ggttatataa cacgtggccg aggtatatca attcatagaa
     4861 aaggttgtcc aaacttaaaa tcacttatag aaaatgaaga ggaaagaata attgatgtag
     4921 aatgggacca acagagatcc gaatcttatg tagtagactt aaatattgaa gcagtagata
     4981 aaagtgccct tttaaatgat attacaaggt taataaaaaa tgaacagatc aagttattat
     5041 ctgttaatgc aagggcagat aaatatggta aagcctatat taaattatct gtcgaactta
     5101 gcagtataga acacatgaaa gatataatga ataagatcaa agatatttca ggtgtattat
     5161 cagtacagag agctaaaccc acttaatata taaggagtga tccagttgcg ggctgttgta
     5221 caaagagtaa aagaaagtaa agttaaggta gaggatgaag taatcggttc tattgaggaa
     5281 ggattgctaa tctttctggg gattggtaat gatgatagta tggaggatat agactacttg
     5341 attgataaaa ttgttaatct tagaatattt gaagatatgg aagggaagat gaatttatct
     5401 gccctcgatt tagaaaaaga aattatggtg gtttcccagt tcacccttta tggggactgt
     5461 agaaaaggta gaagacccag ctttttcgat gcagctcctc ccgataaagc tgaaaggctg
     5521 tatgatgtat ttgtaagcaa aattaaagat tatgagatta atattgaaac aggtgaattt
     5581 caggcaatga tggacgtaga tctcgtaaat gatggaccag taactttatt gatcgatagt
     5641 aaaaagaact tttaatggga ggatttcata tgattatttc acaaacagtt ggtgttaatt
     5701 ttaccaactg ttatttaatt ggaaaaaaga attcgaaagc tatagttatt gatcccggtg
     5761 atgaaattga taagatcatg gctttagtag aaaaaaacaa tgttgaagtt gaaaagataa
     5821 taaatacaca tggacatttt gatcatattt cggctaataa cgaattaaag gaaaaaacaa
     5881 atgccgaagt atatatacat gaaaaagaca aagacgcttt agttaatcct gaaaaaaatc
     5941 tttctactca tttaggacag aaaaatttag ttgaacttaa agaagctgac catattttac
     6001 gggaaggaga tacagtccaa atagaagatt attcttttga agttttacat acgcccggac
     6061 attctcctgg tagtataagt ctttatgata gtaacaagaa aatattaatc agtggagata
     6121 ccatttttag tatgggagtg ggtagaaccg atttcccggg ttgttcgcaa gaagaattaa
     6181 ttaattctat tgaaaacaag ttattaacct tacctgatga aacaaaagta tatcccggtc
     6241 acggccagga gacaaccatc ggaaagttca aagtaaaggt ctgggaacgt ttaacttaat
     6301 tgaatttaag gcgttttatt gttgactttt taaaatcaat aagataaaat tatgaaagat
     6361 gtcaactagc gcttaaaata tatttataca tcttaaggag tgacttatta atgtttgata
     6421 atttaaggaa taatagtaaa attatagtct atatcgtagt agctgctttt gtagttaccg
     6481 gtggattaat gggttttgga tcttacatga gtcgtaataa cccaagtgga agtaatagat
     6541 cctcccaata tatagcaaca gttaatgata aaggtatcac accccagcag tttttatcag
     6601 tactgagaaa ttctgcttct caggcaaata acttaagtca gggtgaggtt ataccattta
     6661 gattaaatgt attaaaatct atgatagaac gagagatctt attatcagag gctgaaaaac
     6721 aggatattaa tgttgaaatc agtgatggag aagtagaaga aagaatagat gaaattttaa
     6781 atcaaaatga tttaacccaa gaagaattga aagataattt ggagagtcaa gactatactt
     6841 atgaacagtt caaaactgat ataagaaata gtttgagaaa ttccgaaaga ataactaagg
     6901 taaaagagac tacttatagt aatattgaag taactgaaga agaaattaaa gaaacatatg
     6961 aagaaagata cgcagatagt gaagaaaagc ccgaattaga agaggttaga tctgatataa
     7021 aagaatcatt attagcaagt aaacaaaatg aggcatatca aaactggtta gaaaacaaaa
     7081 aatcggaagc caatgtaact attaacgacc ctgtcttata tgcctataat gctttagatc
     7141 aaggcaatta tcaggtagct atagacagtt ttcaaaatat ggtaaacagt gaaaatagta
     7201 acacttccgc tagcatatat acttatttag cccaggctta tgcaggggct gaacagtatg
     7261 acaaagcctt agaaactttt aatactgctt tagaagatta tcctgaaaat tgggaattaa
     7321 gattaaactt cggcgaatat tatgctaaca gg
//
LOCUS       DATGCJ010000072        22389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_366073, whole genome shotgun sequence.
ACCESSION   DATGCJ010000072 DATGCJ010000000
VERSION     DATGCJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 22389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22389
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366073"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKN64_03860"
     CDS             complement(<1..412)
                     /locus_tag="VKN64_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59407.1"
                     /translation="MDIIELQEDIYRPEFIYHGNDLGVSYSQEETMIKLWAPTADNVE
                     IEIYNYYKNQEPVEVYDMFEDINGTWTVILEGNYAGKYYLFAVDFGDRVEKTVDPYAK
                     ALAPNSKMGLIVNLNNTNPANWKADSNKKLASPVD"
     gene            complement(448..1851)
                     /locus_tag="VKN64_03865"
     CDS             complement(448..1851)
                     /locus_tag="VKN64_03865"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HMA59408.1"
                     /translation="MNLKEENKIILIYIIIGGLWIYFSDEIIASLFNQGEVLMRIQTY
                     KGLAYVLITGLIFYVLLKNYFKKLRNTREELKKKNEELNKYTSRLEKGQKELQESYED
                     LYDQTEDLKIMIDFINNISKKTFKNTNRFLSTLLRTAYELVPLSDYGSVYKIKNGKIN
                     FVDAIGHDLEKLKELKIDKEVFKTYAAEPQIVDELLMKNDNKLPKEQFNHLIDASQDI
                     KQSLTFYLVVEKDMVAGISLDIDRKSEVEFCDNGVKTLKAFRNLAESFYTFQRYNKLQ
                     GDFQRDIILSMIKFLEIHDKYTGGHSEEVAEYSQEIAEEMDLNESEINDAYWTGLVHD
                     IGKLIVPSVILNKKDDFRKEDYEYIKNHPRWAYKSLSESQRLKDIAEYVLYHHERWDG
                     KGYPEGLEEKEIPLISRIVAVADVYSAMTSDRSYRDALTKEEAIKELKDNKGKQFDPD
                     IVDVFLKIINSDISKAI"
     gene            2001..2978
                     /locus_tag="VKN64_03870"
     CDS             2001..2978
                     /locus_tag="VKN64_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HMA59409.1"
                     /translation="MSLLDSNYTFEDSDIDIISFGELLVDLITEEEEVRLSEAEQFSR
                     YFGGSAANVAVNSQRLGAKTRMLTRIGDDDFGEFLLSVLKRENISNEGIQIDNIRRTP
                     LVFVNKTSGTPAWLAYRDADKYIEFNEKVKELVNKTKIVYLTTFILSQKPSRKTALKV
                     LDKIKKDEDTLLAFDPCYRPILWPEEDDGAQLVKSIISASDFIKPSLDDAEYLFGPDT
                     PENYIEKYLQVGANIVILTMGEDGVLVSDGIEKFRLKVYSKKVVDVTGAGDSFWSGFM
                     IGILDGMSIREAVKLGNATAAFKIRGVGALSQVPEKDYLLKFIKEQEAL"
     gene            2980..4470
                     /locus_tag="VKN64_03875"
     CDS             2980..4470
                     /locus_tag="VKN64_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA59410.1"
                     /translation="MDISEIERVAFLNPQGNFDPKDSYWTEHPDFGGQLVYVKEVCLA
                     LAELGINVDIITRQIKDADWPEFSEPIDRYPESDKVRIVRIPFGGDDFLRKEALWPYI
                     NDYVEGIIDFYEDEDNFPNFVTTHYGDGGLSGVLLKDKKGIPFTFTGHSLGAQKMDKL
                     DVNKVNFTEYNTKFNFHRRIIAERLAMNHADGIVVSTSQERFEQYSHDLYRDAVDTEN
                     DDKFKVIPPGVNTDIFNSEYTEKMKEKLEKYIKRDIDEERRDLPLVISSSRLDQKKNH
                     LGLVKAFAENKQLQKKANLAIALRGIENPFKDYSNADKEEKNILDQVMKIIYNNNCSG
                     KVTMFSLNSQKQLAETYAYFASKKSVFALTSFYEPFGLAPVEAMASGLPAVVTENGGP
                     VEIMNEGEFGVLVDPEEPEDIAAGLLEVLSDEKNWQHYHQKGKKRVMNKYTWEQTAKS
                     YLEFIQDILKTEKEDKEKIDIPDYFTEPDAKNDSRLLDRFLRIWKR"
     gene            4524..6146
                     /gene="glgP"
                     /locus_tag="VKN64_03880"
     CDS             4524..6146
                     /gene="glgP"
                     /locus_tag="VKN64_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HMA59411.1"
                     /translation="MIAKEKRKKVAYFCMEYGINNNFRIYAGGLGILAGDILKAAHEF
                     DYPIFGIGMLWLEGYTKQLIDENGRPYDKYPTNDYIYNHLEDTGVTVTVQVKGNDIKL
                     KVWKMETDYMNPLYLLDSNLEENGKYDWITKRLYAWGEEERIAQEIALGIGGIRAIRA
                     LDLDVDVYHYNEGHAVFAGTEMINEKMEEGLNFDQALEAVRNQTVFTTHTPVKKGNEE
                     HAHHILKDVGAYNGLTEEQMKQIGNDPFSNTVAGLRLAKISNGVAQLHGVTARKMWEH
                     VDNRSPIKAITNGVHRKTWVDSRMFGAFEDGNDLLPVHQQLKSELINYVKDKTGSDLD
                     ANKLLIGFARRAAPYKRADLIFSKMNVIEPYLKDSKIQIIFSGKAHPLDDKGKDLVQR
                     IVKLAEKYPNSVVFLEDYNMEIAKMMTRGVDVWLNNPRRPKEASGTSGMKSAMNGVLN
                     MSILDGWWPEACQHGMNGWQFGDGYVGENQDQHDRDSLYRVLLNEVVPTYYDNKAKWE
                     DMMNNSIESTYEEFSAEKMIENYYLKMYRKIR"
     gene            complement(6279..7088)
                     /locus_tag="VKN64_03885"
     CDS             complement(6279..7088)
                     /locus_tag="VKN64_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMA59412.1"
                     /translation="MEKLIKVGLIQFESLLADIEENKNKSFKLMDKTVKLGAQIICFP
                     EMFNTGYNLDLLGNEIYNLAVTQNSEYIKEYCQYAKNNNVHIILPLTIKDERENIFNS
                     ALMIDNKGKIKGKYDKTHLFLHEKKYYQAGNNFTIFNIDNIKIGILICYDLGFPEAAR
                     YLTLKGADIIFVPSAWRIQDYDIWDLNTRQRALENNIFIVGVNRVGKEADLHLFGNSR
                     IIDPNGEIMMEAPRDEETIIVKELDLSKISKYRNYYQYLKARRNDLYGDFK"
     gene            complement(7106..8092)
                     /locus_tag="VKN64_03890"
     CDS             complement(7106..8092)
                     /locus_tag="VKN64_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59413.1"
                     /translation="MPDSLIEVNKLKKYFTIKKGMIKSSETTVKAVNNITFDIKKGET
                     FGLVGESGSGKSTVGNCITGLYKPTSGEIIYKGQRIDNVPLQKKNKNKAREGIQMVFQ
                     DPTSSLNPRRTIRQILKLPLKKYYNLKDKSEIDKEIYSLAEKVELTSENIDKYPRSLS
                     GGQKQRVAIARTLACNPDFIVLDEPTSALDVSVQAKVINILDHLKTKMNLSYLFITHD
                     LSLMRNIANRTAVMYLGQIFELADNTELFHNPSHPYTRTLLSAIPVITEEEEKIRPKD
                     IEVQGEIPSASDIPKGCSFHNRCSKAFDLCYENEPDMIEISPGHKVKCHLYK"
     gene            complement(8085..9119)
                     /locus_tag="VKN64_03895"
     CDS             complement(8085..9119)
                     /locus_tag="VKN64_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59414.1"
                     /translation="MLKYNNLLEINDLYVNFERYAGKAEVLNGVNLSVNASEKVGLVG
                     ESGCGKSLTVKTIMNLLPEKRIEVEGSIKYKGKDILNLSHESRKKIIGKEVVMIFQDP
                     MSSLNPVFKVGQQMEENLWWNHYLDAKPSSFLKRRFKKEKREKLRNTIIKTLNEVRIP
                     DTERVLNSYPFQLSGGMRQRILIAMALASEPTLLLADEPGTALDVTTQKQIITLLNEL
                     VDKKDLAILMITHNLGLIRETTHRVYIMYGGNIVESAKTKEIFNNPLHPYTEGLLASV
                     PKLTAEGVGEGIEGTVPNYLNIGTNCRFEPRCKYATKRCKNEKPKMFEIKPEHFVACF
                     NPLERGDKHA"
     gene            complement(9106..9963)
                     /locus_tag="VKN64_03900"
     CDS             complement(9106..9963)
                     /locus_tag="VKN64_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59415.1"
                     /translation="MKFNLIENFQKNWYKFYQNKTSVVGLIIVITIVLMAIFAPYVTP
                     YPQQAGVFTDFNNTFKSPSFNYIFGTDEVGRDVFTRIIFGFRFSLILAATVLLIGVPL
                     GTILGIMAGYFGGWVEILIMRFTDMMLSIPPLVMALSISAVLSPNLRNMMIALAAIWW
                     TWYTRLVRSIVVSLRNENFIKALKIMGIGDFHIMFVEILPNCVSQILVKMTLDIAFII
                     QLGAGLSFLGLGAQPPTPALGTMISYGTTHLPTFWWLTVFPALGILFLVFGFNWLADG
                     LKDVLDVEV"
     gene            complement(9965..10987)
                     /locus_tag="VKN64_03905"
     CDS             complement(9965..10987)
                     /locus_tag="VKN64_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59416.1"
                     /translation="MKYFGYLLKRIAYSLIVLLGLSIIIFFISRVLPGDPARMALGPY
                     ASQEQVNILRESMGLDKPLFVQYYTYITSLFRGDFGMSLITKRNVLLDLQYHLPATLE
                     LIFFTIFWVVFFGIPLGVITAANKDSTLDNIFKTFSFAAVVTPGFIVGLAFQLIFGYG
                     LDIMPITGRLSSALASPPNITGLLLFDSFIQGQWEVFFNALRHILLPSLALSMAGIGQ
                     VSRITRATMIEIGEKDYVAVMHSYGVPKSIVNFIYMLKPSFIPSLNVIGMTFASLLGN
                     AFLIEMVFAWPGIAKYGINALLRTDINALMGVVLLIGAFFLLTNFIVDIIMGLLDPRI
                     SLMGGE"
     gene            complement(11079..12641)
                     /locus_tag="VKN64_03910"
     CDS             complement(11079..12641)
                     /locus_tag="VKN64_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59417.1"
                     /translation="MSRRVLSTVVVFILLSALMIFTTGVIAQKNIVGMSLNQEISTID
                     PSKTTDWTESMVIVNLYDSLVTPNPDGTMAPLIAEDWDYTEDGLNYTFDIKKGIKFHD
                     GSELTAEDVKFSFDRMLELKQGYSWLWLDVLNEVKVTDDYTVEITLNRPYSPFVSTLP
                     WLAILNKEQVMANEVEGDMGQDWLLDHDAGSGAYKFKSWERGNQIVFEKFDDYFQGWR
                     DNSIDEVRARVIYSDSTVLSEMKTGDLTIADHYRALETYDRLDNLEGVKIQKAQSGEI
                     FYLKINTQKPPVDNIHVRKALSWAFDYETFNTKIEPGTEQSRGPVPKIIAGHNPDVFQ
                     YEKNLDKAKEELAKSGFEPGELTITYCYVEGFEMERKLGLMYQADLAEVGINLELQPE
                     TWGRITDLASNVESTPHITAVFSAANYPDPDNYLYTAYHSSASGTWMSMEWLQNEEID
                     KMIMEARRTLEKEKRNEIYYEIQDKIVNLAPDIFIYSLLKRYGMQEHLKGFEFVPVMS
                     FEYDFHKLYFEE"
     gene            complement(12688..13623)
                     /locus_tag="VKN64_03915"
     CDS             complement(12688..13623)
                     /locus_tag="VKN64_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HMA59418.1"
                     /translation="MILKQILEIFELLDKPNSNGAEMADFLKERGAENVYVTSIQGKN
                     GYTDGIKIYIAGKNGKKNGGNAPTLGIIGRLGGIGARPLVKGFVSDGDGALTVLSAAL
                     KLIEMKTKGDQLEGDIIITTHICPNAPTIEHKPVPFMGSPIDMRAMNEHEVLPEMEAI
                     LSVDTTKGNKIVNSNGFAISPTIKEGYILKISDDLLTIMEKVTGNLPLTIPVSQQDIT
                     PYGNDLFHINSLYQPSTATKSPLIGVAITTGVPVAGCASGASHFGDIEKAGRFLIETA
                     KAYGNLECEFYDRAEFEKILKLYGKMNRFQTMGIK"
     gene            complement(13640..14293)
                     /locus_tag="VKN64_03920"
     CDS             complement(13640..14293)
                     /locus_tag="VKN64_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HMA59419.1"
                     /translation="MKKYTVGIIRVLSTENEEIKYKHGKIIERSFPMFKTKTFCIENQ
                     PEGINSKAAHDKAVPKIINLGLKISQEVDGIIISCAEDPAVKELDNKLDIPVIGPGRA
                     ASVTSIFYGEKIAVLSLLEDIPSVYKDILKDKLIGIIYNPEIKESMDLVNKNKLNKLI
                     EKVNNFESNFDVLTLSCTGMSTVGVSDILRKKFKIPVIDPLMSAGLYMYYSLKKDTY"
     gene            complement(14420..16072)
                     /locus_tag="VKN64_03925"
     CDS             complement(14420..16072)
                     /locus_tag="VKN64_03925"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HMA59420.1"
                     /translation="MELTVREILNMDFMQDAEIIAGKDSIERFVKYVDVMEVPDIDEW
                     LREGVFLLTTGYNIKDDLELQERMIKKMSEVKAGALCIKEGRYLEKIPDFLINLGEEY
                     NLPIIKIPKDVHYNSIMLPLFSKILGKKNNSISRFENIHNELVKVILKGGGIDLLAQT
                     LHKLIEKPVLIQDKNLKTLALIGEYNEEKKIKKNISITDKKLLNLFTFNRKATRIKYE
                     DATIARVVAPIFVTDKIYGFVSAFEIDNNKLDNIDFRAVELTSTNIALELLKEKDKEA
                     TQNRLKEELIEDLINDNYNDEKTIYQRAKYFGWDLRSDYHVIVIDIDNFEEYYLSLKN
                     KDEDVMQRIKENIKSIIKWELMMLNKDIIIINQSDSFVVLYKSNKVKNELKDKKELEN
                     FCHKIKSSIKQQISHITVSIGIGNLYKGIDGLKKSYKEAFKALSMGKNIYGQNKITRY
                     DDLGIYRVIAKLNDDPDLIDFYEEVIGSLVNNPKNEELVDTVESIIKNFGNKTAAAKG
                     LCIHRNTLNYRIKKIEKILEVDLDNPENYLNLYMALKIKQLL"
     gene            complement(16773..18089)
                     /locus_tag="VKN64_03930"
     CDS             complement(16773..18089)
                     /locus_tag="VKN64_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA59421.1"
                     /translation="MIDIDFLVNSIEKELINLRRKFHSHPETGWLELYTTAEIITYLK
                     ELGYEVKYKEDILNTEYRQGLPNIEDFEKAYKRAVDWGTEPQILEEVKAGMTGAAAIL
                     DTNKEGPITAFRFDIDAIGSEESNADSHRPVRENFRSINPQAVHSCGHDGHIAIGLGL
                     AKLLKELSAKDKLKGKIILIFQPAEEGVRGGKAVAESELLNDVDYFLSGHIGMNADDS
                     NSIICGITGSLYTKKFDINITGEPSHAGGAPEKGKNALLAANNLISSLYSLNQHGKGM
                     SRVNVGKMEAGTYRNLIPANAYLQMEIRGENDEVISYLDNKVKNILKGIEKIYDVNLE
                     LEVVGEAISMESDNELINIVKEAAQDIEDIGNIIDKKSMQGSEDASFLMDKVQKQDGK
                     ACYINFGTELAAGHHQDNFDFDESVLARAVKLYLYSLKELGNIKVL"
     gene            complement(18176..19351)
                     /locus_tag="VKN64_03935"
     CDS             complement(18176..19351)
                     /locus_tag="VKN64_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA59422.1"
                     /translation="MKAYKNLDYIDVENEVVIEDAVIIVENEKFKKVGRDIEIPKEAE
                     VIDLEGKTVLPGIIDAHIHSMLDASGDPHTTMENENDAVTIIKAQKALEKTLKAGVTY
                     VRDLGGAEHYDLGLREAASRGIFNGPRMQVAGRVITMTGGHGYRMGRETDGVADARKA
                     AREQLKAGVDVVKIMSTGGVMTKGVEPGSPQLSFEEIQAAVEEAHKACRKTATHAQGT
                     VGIKNAIRAGIDSIEHGFFLDQEAVDMMKERGTFLVATLVAPYWIIEKGVENGVPEYA
                     VEKSKKVIKKHFESFEFAFKNDVKIAMGTDAGTPFNEHGKNTYELKLMVEAGMPPIKA
                     IKAATLGGAELLDILDDFGSIEEGKVADMVVVKGNPAENIEDIFNVESVFKAGEIVV"
     gene            complement(19394..20359)
                     /locus_tag="VKN64_03940"
     CDS             complement(19394..20359)
                     /locus_tag="VKN64_03940"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HMA59423.1"
                     /translation="MELLQVEHLKKHFSVNDGFWANLFNNDNQVHAVNGVSFTIKKGE
                     TLSLVGESGCGKSTIGRTILGLYEPTEGEISYDGKKINDMSTKEKFGLKEKMQMIFQD
                     PFSSLNPRQKVKDIIKEPIRTHNIVSREEEDDYILNLLDKVGLDGSYLNRYPHQFSGG
                     QRQRIGIARSLSMEPEFIVADEPISALDVSIQAQILNLLMDLQDEFDLTYLFIAHDLS
                     VVKHISDRVAIMYLGKVFEIGDKEKIFSNPIHPYTKGLFNSIPELTTKTKEEFSVLSG
                     DVPDPIELPGGCFFHSRCKYAEDICLREKPELKKIEGREVACHLY"
     gene            complement(20361..21362)
                     /locus_tag="VKN64_03945"
     CDS             complement(20361..21362)
                     /locus_tag="VKN64_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59424.1"
                     /translation="MQKEKILEVNNLRTHFNTKEGVVKAVNGVSFNLHKGEILGIVGE
                     SGAGKSVTGFSLLRLIDSPGEIVEGEIKYKDTDLLKMNKKRMNKIRGEKISMIFQDPM
                     ASLNPVLTIGRQLMEVLQFHLNYSRKEAKNRAIELLEMVGISSPETRLKHYPNQLSGG
                     MRQRVVIAIALASDPEIIIADEPTTALDVTIQAQIIGLMKNLIKDRQTSLIFITHDLA
                     LVSEIADRIMVMYAGDVIEHGRKSKIISNPVHPYTKGLIKSIPMMDRDQKRLEQIKGL
                     MPSPLDLPDGCKFHSRCSRKSHRCRNYNNELIEIEKNHFATCSEARQIKNDLIAGVG"
     gene            complement(21405..22262)
                     /locus_tag="VKN64_03950"
     CDS             complement(21405..22262)
                     /locus_tag="VKN64_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59425.1"
                     /translation="MEDLIYYFKRDKLVIIATIILVLIIAASIFAHIIAPHNPYDLAS
                     VSLMDSMKPPVWMEGGVDKFILGSDLQGRGILSTILYGTRISLLVGFAAVALGGTIGI
                     TLGMIAGYFGGKVDSIIMRMADIQLSFPTMLIAITVMAFLGRGILKLIVVIGLATWVT
                     YARTARGSTLSIKSSEFVEAAKLIGVTNTKILLRHILPNILTPIIVIATVQIAQVIML
                     EATLSFLGIGVPLTEPSLGLMIADGYKRLLSGKWWLVVFPGGFLVMIVLSINIIGDWL
                     RDVLNPKLK"
BASE COUNT         7244 a         4195 c         3244 g         7706 t
ORIGIN      
        1 catcaaccgg actggctaac tttttattgg aatcagcttt ccagttggcc gggttggtat
       61 tattcagatt tacaattaac cccattttac tgtttggcgc taaagccttc gcataaggat
      121 cgacagtttt ttcaactcta tctccaaaat caacggcaaa taggtagtat ttgccggcat
      181 aatttccttc aagtatgact gtccaggtgc catttatatc ttcaaacata tcataaactt
      241 caacaggttc ttgattttta taatagttat aaatttcaat ctcaacattg tcagctgtgg
      301 gtgcccacag tttaatcatg gtctcttctt gcgagtagct gactcctagg tcattaccat
      361 gataaataaa ttcaggtcga tatatatctt cctgcagttc tataatatcc atttattata
      421 actccctcat aaaactatta tttttaatta gattgcttta ctaatatcac tatttattat
      481 ttttaaaaat acatctacta tatcgggatc aaactgttta cctttattat cttttaattc
      541 tttaattgct tcttctttag ttagagcatc acgataagaa cggtcggaag tcatggctga
      601 ataaacatcg gccactgcca ctatccggga gataagaggt atctctttct cttctaaacc
      661 ctcgggatac cctttcccat cccacctttc atggtgataa agcacatatt cggcaatatc
      721 ttttaatctc tgcgattcag atagtgactt atatgcccag cggggatggt ttttaatata
      781 ttcataatcc tcttttctaa agtcatcttt tttatttaaa ataacactcg gaacgattaa
      841 ctttcctata tcatgaacca agcctgtcca ataggcatca tttatttcac tttcatttaa
      901 atccatctcc tcagctatct cctgagagta ttctgcaact tcctcagaat ggccaccggt
      961 atatttgtcg tgtatttcta aaaacttaat cattgataag attatatcac gttggaagtc
     1021 tccctgcaat ttattatacc tctggaaggt ataaaaagac tctgctaagt tgcgaaatgc
     1081 ctttaaagtc tttactccat tatcacaaaa ttctacttct gacttgcggt ctatatcaag
     1141 tgaaataccc gcaaccatat ctttttctac caccagataa aaagttaagg actgtttgat
     1201 gtcttgggaa gcatcaataa ggtggttaaa ctgttctttc ggtaatttat tatcattttt
     1261 cattaaaagt tcatcaacaa tctgtggttc agctgcatag gttttaaata cttctttatc
     1321 tatcttaagt tcttttaact tctctaaatc atgtccgatc gcatcaacaa aattaatttt
     1381 accgtttttg attttataga cactgccata atcgcttaac ggaactaatt cataagcagt
     1441 tcttaacaaa gttgataaaa atctattggt atttttaaag gtttttttgc ttatattatt
     1501 aataaaatct atcattattt ttaagtcttc agtctggtca taaagatctt cataagattc
     1561 ctgcaattct ttttgaccct tttctaaccg ggatgtgtat ttgtttagtt cttcattctt
     1621 cttttttaat tcttctcttg tatttcgcag ttttttaaag tagtttttca ggagcacata
     1681 aaatattaga cccgttatta atacataagc gagcccttta taagtttgga tgcgcattag
     1741 tacttctccc tgattaaaaa gggacgcaat tatctcatcc gaaaagtaaa tccataaacc
     1801 accaattatt atatatatta aaatgatttt attttcttct tttaaattca tttagatcat
     1861 cccatatttt ggctcttata gtaaagataa acactccttc taatttataa taattcaaga
     1921 aatcctatat aataccttca agcaggtaaa taaatatttt tctagaataa tcaattaaga
     1981 aggtaaaaga ggtgaaatta atgtcattac tagattctaa ttatacattt gaggattctg
     2041 atattgatat aataagcttt ggcgaactat tggtagactt aattacagaa gaagaggaag
     2101 ttagactatc agaagcagaa cagttttcac gttacttcgg ggggtcagcc gccaatgtgg
     2161 ccgttaattc tcagcggttg ggggcaaaaa ccaggatgtt aaccagaatt ggtgatgatg
     2221 attttggtga gtttttattg agtgtcttaa aaagggaaaa tattagtaat gagggaatcc
     2281 agattgataa tattagaagg actccccttg tttttgtgaa caaaacgagc ggtacaccgg
     2341 cctggcttgc ttaccgtgat gccgataaat atattgaatt caatgaaaaa gtaaaagaac
     2401 tggttaataa aactaaaata gtatatttaa caacctttat tctctctcaa aaaccttcgc
     2461 gcaaaactgc cttaaaggta ttagataaga taaaaaaaga tgaagatact ttactggctt
     2521 ttgacccctg ttaccgacct attctctggc cggaagaaga tgacggggct caacttgtta
     2581 agtccataat ctcagcctcg gattttatta aaccttcttt agatgatgcc gagtatcttt
     2641 ttgggcctga tacacccgaa aattatatag aaaaatattt gcaagtagga gcaaacattg
     2701 ttatattaac tatgggtgaa gacggtgttt tagtatcgga tggaatcgaa aaattcagac
     2761 tgaaagttta ttctaaaaag gtagtagatg ttactggtgc aggtgactct ttttggtctg
     2821 gttttatgat tggaatatta gacggtatga gtattagaga agctgtgaaa ttaggtaatg
     2881 caactgcagc ttttaagatc agaggtgtag gtgccctttc acaggtacca gaaaaagatt
     2941 atcttttaaa atttattaaa gagcaggagg cattatagta tggatatatc tgaaatagaa
     3001 agagtggctt ttttaaatcc acaaggtaac tttgatccca aagatagtta ctggactgaa
     3061 catcccgact ttggcgggca gttagtctat gtaaaagaag tttgcctggc tttagctgaa
     3121 ctgggaatta atgtagatat tattaccaga cagatcaaag atgcagactg gcctgaattt
     3181 tcagaaccta tagatagata tcccgaaagt gataaagtaa gaattgtgag aatacctttt
     3241 gggggagatg acttcttaag aaaagaagcg ctctggccct atatcaatga ttatgttgaa
     3301 ggtattatag atttttatga agatgaagat aactttccga actttgtgac cactcattat
     3361 ggtgacggcg ggctcagtgg agtcttacta aaagataaaa aaggaatccc cttcactttt
     3421 accggccatt ctttaggtgc acagaagatg gataagttgg atgtaaataa ggttaatttt
     3481 accgaatata atactaaatt caactttcat cgcagaatca ttgccgaaag actcgcgatg
     3541 aaccatgcgg atggcattgt ggtcagcacc tcgcaagaaa gatttgaaca gtactcccat
     3601 gatctatata gagatgctgt agacactgaa aatgatgaca aatttaaagt tatccccccg
     3661 ggagttaaca cggatatctt taatagtgaa tatacggaaa agatgaaaga aaaattagaa
     3721 aaatatatta aaagagatat cgatgaagaa agaagagatt tacctttagt tatctcttcc
     3781 agccgtttag atcaaaagaa aaaccattta ggcttggtca aagcttttgc tgaaaacaag
     3841 cagcttcaga aaaaggcaaa cttagctata gctttacgcg gaatcgaaaa tccctttaaa
     3901 gattattcaa atgccgacaa ggaagaaaaa aatattcttg accaggtcat gaagattatt
     3961 tataataata actgttcggg taaagttact atgttttcct taaatagtca aaaacagtta
     4021 gctgaaacct atgcttactt tgcaagtaaa aaatcggtct ttgctttaac ttccttttat
     4081 gagcccttcg gattagctcc agtggaagca atggcatccg gactgccagc agttgtaact
     4141 gaaaacggcg gcccggttga gatcatgaat gaaggtgaat ttggtgtatt agtcgatccc
     4201 gaagaacctg aagatattgc agccggtcta ctagaagtat tatctgatga aaaaaactgg
     4261 caacattacc atcaaaaagg taaaaaaaga gttatgaaca aatatacctg ggaacaaact
     4321 gctaaatcat atctagaatt tatacaggat atattaaaaa cagaaaaaga agataaagaa
     4381 aaaattgata ttccagatta ttttacagaa ccagatgcca aaaatgattc caggttactg
     4441 gatagatttt taagaatctg gaaaagataa taagaaataa aataaaatat aagtataata
     4501 ttactttggg agagtgattt aatttgatag caaaagaaaa acgaaaaaaa gtagcatact
     4561 tctgcatgga gtacggcata aacaataact tcagaatata tgcaggcgga ttagggattt
     4621 tagccggtga tatattaaaa gcagcacatg agtttgatta ccctattttc ggaatcggta
     4681 tgctctggct ggaaggttac acaaaacagt tgattgatga aaacggtcga ccctatgata
     4741 aatatcctac caacgattat atatataatc atctagagga tacaggcgtg actgttactg
     4801 tgcaggtaaa aggtaatgat ataaaactta aagtatggaa gatggaaact gattacatga
     4861 atccgcttta tctcttggat tctaacttag aagaaaacgg aaaatatgac tggattacaa
     4921 aaaggcttta tgcctggggt gaggaagaaa gaattgccca ggaaattgca cttggtattg
     4981 gaggaattag agcaatccga gctcttgact tagatgtgga tgtctatcat tataatgaag
     5041 gtcatgccgt tttcgccggt acagaaatga tcaatgaaaa gatggaagaa ggtcttaatt
     5101 tcgaccaggc tttagaagct gttagaaatc aaactgtctt tacaactcat actcctgtta
     5161 aaaagggtaa tgaagaacat gcacaccata tcttaaagga tgtaggggct tataatggac
     5221 taactgaaga gcagatgaaa cagatcggta atgacccctt cagtaataca gtggccgggt
     5281 taagattggc taagatctca aatggagttg cccagttaca tggggtaact gcccgtaaaa
     5341 tgtgggaaca tgtagataac aggtctccca ttaaagctat tacaaacggt gttcatcgaa
     5401 aaacctgggt agattcccga atgttcggtg cctttgaaga tggtaatgac ttactaccgg
     5461 tccatcaaca gttgaaatct gaactgatta attatgttaa agataaaacc gggtcggatt
     5521 tagatgctaa taaactatta attggttttg ccagaagggc tgctccctat aagagagccg
     5581 atcttatctt cagtaagatg aatgtaattg aaccatactt aaaggatagt aagattcaaa
     5641 ttatattctc gggtaaggcc caccccttag atgataaagg taaagatcta gtgcaaagaa
     5701 tagttaaatt agctgaaaaa tatcccaata gtgtggtctt tttagaagat tataacatgg
     5761 aaattgccaa gatgatgacc agaggagtag atgtctggct gaacaatcca cgcagaccga
     5821 aagaagccag cggtacttcc ggtatgaaat ccgctatgaa tggtgtgctt aatatgagca
     5881 tcctggacgg ctggtggccg gaagcctgtc aacacggcat gaacggctgg cagtttggtg
     5941 atggttatgt gggagaaaat caggatcagc atgacagaga ttctctctat agagtcttac
     6001 tcaatgaagt tgttcctact tattatgata acaaagcaaa atgggaagat atgatgaaca
     6061 atagtattga gagtacttat gaagaatttt cagctgaaaa aatgatagaa aactattatc
     6121 taaagatgta taggaaaata agataataat taaaaaactc acctctgaaa attgaggtga
     6181 gttttttgtt tgaatatatt tttttatccc aatattttaa acaaaataaa agaaaatttt
     6241 atgcttaata atagcacttt ttataaacag caaatcaact acttaaagtc cccatataaa
     6301 tcgttacgcc ttgcctttaa atattgataa tagttcctat atttcgatat tttagataaa
     6361 tctaattctt ttacaataat tgtttcttca tctctgggtg cttccatcat tatttcgcca
     6421 ttgggatcaa taattctact atttccaaat aaatgaaggt cagcctcttt cccaactcta
     6481 tttactccta ctataaaaat attgttttct aatgctctct gtcgggtatt tagatcccaa
     6541 atatcatagt cctgaattct ccaggcagag ggtacaaaaa taatatcggc accttttaat
     6601 gttaaatatc gagctgcttc tggaaaaccc aaatcatagc atattaatat accaatctta
     6661 atattatcta tattaaagat tgtaaaatta ttaccagctt gataatactt tttttcatgt
     6721 aaaaaaagat gtgttttatc atatttccct tttattttcc ccttattatc tatcataaga
     6781 gccgaattaa aaatattttc tctttcatct ttaatagtta atggtaatat tatatgaaca
     6841 ttattatttt tagcatactg acaatattct tttatatatt cactattttg tgtaactgcc
     6901 aaattataaa tttcatttcc taacaaatct agattatagc ctgtattaaa catctcagga
     6961 aaacaaatta tttgagcccc taatttaact gttttatcca ttaatttaaa tgacttattt
     7021 ttattttctt caatatctgc cagcaaactt tcaaattgta taaggcctac ttttattaat
     7081 ttctccaatt taaaactcac cccatctatt tatataaatg acatttcact ttgtgacccg
     7141 gacttatttc aatcatatca ggttcgtttt cataacaaag atcaaaagcc ttactacatc
     7201 tattatggaa actacatcct ttgggtatat cagaagcact gggaatttca ccctgtactt
     7261 caatatcttt aggccttatt ttttcttcct cttctgtaat cactggaatt gcacttaaca
     7321 atgttctcgt atatgggtga ctaggattat gaaaaagttc tgtattatct gctagttcaa
     7381 atatctgacc caaatacatt acagctgttc tgtttgcaat atttctcatt agactcaaat
     7441 catgagtgat aaacaaatag gacaaattca tctttgtttt taaatgatct aaaatattta
     7501 taactttagc ctgcactgat acgtccaaag cagatgtagg ttcatctaat actataaaat
     7561 caggattaca ggctaatgtc cttgctatag ctactctctg tttttgtcca ccacttaatg
     7621 accttggata tttatctata ttttctgatg ttaactctac tttttccgct aaagaataaa
     7681 tttctttatc aatttcactt ttatccttta aattataata ttttttaaga gggagcttta
     7741 aaatttgcct aatagtcctt cttggattta aagatgatgt agggtcttga aaaaccattt
     7801 gaatgccctc tctagcttta tttttatttt tcttttgcag gggtacatta tcaattcttt
     7861 gacctttata aattatctct cctgaagttg gtttatataa accagttata caatttccta
     7921 cagtgctttt accagaacct gattcgccaa caagaccaaa agtttctcct tttttaatat
     7981 caaaagtaat attattaact gctttgacag tagtttcaga acttttaatc attccttttt
     8041 taatagtaaa atattttttt aatttattca cttcaattaa tgaatcaggc atgcttatct
     8101 cccctttcta aggggttaaa acaggcaaca aaatgctccg gttttatttc aaacatctta
     8161 ggtttttcat ttttgcatct tttagtagca tatttacagc ggggttcgaa cctacaattt
     8221 gtaccaatat ttaagtaatt tggaaccgtg ccttcaattc cttctccgac tccttcggct
     8281 gttaattttg gcactgaagc taataacccc tcagtatatg ggtgtaaggg attattaaat
     8341 atttcttttg ttttagcgct ttctacaata ttgccaccgt acattatata tacccgatgt
     8401 gtagtctctc gaattaatcc caaattatgg gtaatcatca aaattgccag atctttttta
     8461 tcaactaatt catttaataa tgtgattatc tgtttttgtg tagttacatc aagtgcagta
     8521 cccggttcat cagccaaaag aagagttggt tcactggcca aagccattgc aattaatatt
     8581 ctctgtctca tgccaccact taattggaag ggataactat ttaatacgcg ttctgtatca
     8641 ggaattctaa cttcatttag ggtttttatt atagtatttc ttaatttctc tcgcttttct
     8701 tttttaaatc ttctctttaa aaaagaagat ggtttggcat ccaaataatg attccaccat
     8761 aagttttctt ccatttgttg gccaactttg aaaaccggat ttaaagagga cataggatct
     8821 tgaaatatca ttactacctc ttttccaatg atcttttttc tggattcatg tgataaattc
     8881 aagatatcct ttcccttata tttaatacta ccttctactt ctattctttt ttcaggtaat
     8941 aagttcatga tagtctttac tgttaaagat ttgccacaac cactttcacc tactaagcct
     9001 accttttcac ttgcattgac agataggttt acaccattta atacttcagc tttgcccgca
     9061 tacctttcaa aattcacata taaatcattt atttctaata aattattata cttcaacatc
     9121 aagcacatcc tttagaccat ctgccaacca gttaaaacca aatactaaaa ataatattcc
     9181 cagagcggga aagactgtca accaccaaaa agttggtaga tgggtagtac cataagaaat
     9241 catggttcct aaagctggag taggaggttg ggcacctaac ccaagaaaac tcaaacctgc
     9301 tcctaattgg atgataaaag caatatctaa tgtcattttt actaaaatct gagaaacaca
     9361 gttaggtaaa atttcaacaa acataatatg gaaatctccg attcccatta tttttaatgc
     9421 tttaataaaa ttttcattcc gtaatgaaac tacaatagat cttactaatc tagtgtacca
     9481 ggtccaccaa atagcagcta gcgcaatcat catattcctt aaattcggtg ataataccgc
     9541 actaattgaa agcgccataa ctaggggggg aatacttaac atcatatcag taaatctcat
     9601 aattaatatc tctacccagc ccccaaaata accagccatt atacctaata tggttcctag
     9661 aggaacccct attaaaagaa ctgttgcggc taagatcaaa gaaaacctaa aaccaaatat
     9721 tattctagta aatacatctc tccccacctc atcagttcca aaaatataat taaaactggg
     9781 actcttaaat gtattattaa aatctgtaaa tacgcctgct tgttgtggat agggggtcac
     9841 ataaggagca aatattgcca ttaaaactat agttattact attattaagc ccacaactga
     9901 tgttttgttt tgataaaatt tataccagtt tttttgaaaa ttttctatca aattgaattt
     9961 caatttactc ccctcccatt aaactaattc ttggatctaa taagcccata ataatatcaa
    10021 ctataaaatt agttaataag aaaaaggccc ctattaacaa aacaactccc atcaatgcat
    10081 taatatcagt tcttaataaa gcattaattc catattttgc tataccaggc caggcaaaaa
    10141 ccatttctat taaaaaagca tttcctaata aagaagcaaa agtcatccct ataacattaa
    10201 ggcttggaat gaaagaaggt tttaacatat agatgaagtt gactatcgat tttggtactc
    10261 catatgaatg cataacagct acataatcct tttcaccaat ttcaatcatg gtggctcttg
    10321 ttatcctaga gacctgtcct ataccagcca ttgataaagc tagagaaggt agtagtatat
    10381 gtcttaaagc attgaagaaa acttcccact gaccttgaat aaaagagtca aataataaaa
    10441 gcccggttat attaggagga ctggccaacg cagaactaag ccgccctgtt attggcatga
    10501 tatctaaacc atatccaaat attaattgaa atgctaggcc aactataaaa ccaggtgtaa
    10561 caacagctgc aaatgaaaaa gttttaaaga tattatctaa agtactatct ttgttagcag
    10621 cagtaatgac cccaagtgga attccaaaaa agacgaccca aaatatagtg aaaaaaatta
    10681 attctaaagt agcaggaaga tggtattgta aatcaagcaa tacatttctt ttagtgatta
    10741 aggacatgcc aaaatctcct ctaaaaagac tggtaatata tgtatagtat tgaacaaata
    10801 gaggtttatc aagccccata ctttctctta atatatttac ctgttcttgt gaggcatatg
    10861 ggcctaaagc cattctagct ggatccccgg ggagtactct tgaaataaaa aatattatta
    10921 tagaaagtcc tagaagtact attaatgagt atgcaattct tttcaataaa taaccgaaat
    10981 atttcattta aacctcccct tgttcttaat taattttatc agaccgacct aaataaaggt
    11041 cggtctgact tcaaggaaac aattttttaa gctaataatt attcttcaaa atataatttg
    11101 tggaaatcat actcaaaaga cataacaggt acaaattcaa aaccctttag atgttcctgc
    11161 ataccatatc ttttaagtaa tgagtagata aatatatctg gtgctaagtt aacaatttta
    11221 tcttggattt catagtaaat ttcgtttctt ttttctttct caagtgttct tctcgcttcc
    11281 ataatcattt tgtctatttc ttcattctgt aaccattcca ttgacatcca tgtgcctgac
    11341 gccgaagaat gataagctgt atatagatag ttatcaggat ctggataatt agccgcacta
    11401 aatactgctg tgatatgagg ggtactttct acatttgaag ctaaatcagt tattctaccc
    11461 caggtttcag gttgtagttc aagattaatt ccaacttcag ccaggtctgc ttgatacatt
    11521 aaacccaatt ttctttccat ttcaaaacct tcaacataac aataggtaat agttaattca
    11581 cccggttcaa atccactttt agcaagctct tcttttgcct tatctaaatt tttctcgtat
    11641 tggaatacat ccggattatg tccagcaata attttaggta ctggtcctcg cgattgttca
    11701 gttcccggtt ctatttttgt attaaaagtc tcataatcaa aagcccaaga aagagctttc
    11761 cttacatgaa tattatcaac ggggggtttt tgggtgttaa tcttcagata aaaaatttca
    11821 cctgactgtg ccttctggat tttaactcct tccagattgt ctaatctatc atatgtttct
    11881 aaggctctat aatggtctgc aatagtaaga tcacctgttt tcatttctga gagaactgtt
    11941 gaatctgaat aaatcactct agctcttacc tcatcaatag aattatctcg ccaaccttgg
    12001 aaataatcat caaacttttc aaaaactatt tgattacctc tttcccaact cttaaactta
    12061 tatgctccag aacccgcatc atgatctaat aaccaatcct gccccatatc tccttcaact
    12121 tcattagcca ttacctgttc tttatttaaa atagccaacc agggaagtgt ggaaacaaaa
    12181 ggagaataag gtctatttaa agttatttca acagtataat catcagtcac tttcacttca
    12241 tttagaacat ctaaccatag ccaagaataa ccctgtttta attccaacat cctatcaaaa
    12301 gaaaacttga catcttctgc agttagctca ctaccatcgt gaaatttaat tccttttttg
    12361 atatcgaaag tataattgag gccatcttct gtataatccc aatcttctgc aattaaaggt
    12421 gccattgtac catctggatt aggagtaact aaagaatcat aaagatttac gataaccatt
    12481 gattctgtcc aatcggttgt tttagaggga tcaattgtac taatttcttg gtttagactc
    12541 attccaacta tgtttttttg agctatcact ccagtggtaa aaatcattaa agcagataat
    12601 aggataaaaa caacaacagt acttaatact cttctactca taaaattttt cctccttttt
    12661 aatttttaaa atcttacttt aaatttacta ttttataccc atcgtttgaa agcgattcat
    12721 cttcccatac aattttaata tcttctcaaa ttctgcccga tcataaaatt cacattctaa
    12781 attaccataa gctttggctg tttcaattaa aaatctaccc gccttctcga tatctccaaa
    12841 atgacttgcc ccacttgcac aaccggctac tggtactcca gttgtaatgg ccacccctat
    12901 taatggtgat ttagtagcag ttgaaggttg gtataagctg ttaatatgaa ataaatcatt
    12961 tccatatgga gttatatcct gctggctaac cggaattgtt aaaggtaagt taccagtaac
    13021 cttttccatt attgttaata agtcatcact gatttttaat atataacctt cttttattgt
    13081 aggagaaata gcaaaaccat ttgaattcac aatcttatta cctttagtag tatcaacaga
    13141 taatatagcc tccatttcag gtaaaacctc atgttcattc atagcacgca tatcaatagg
    13201 tgaacccata aaaggaaccg gcttatgttc aatagtagga gcgtttggac atatatgagt
    13261 tgtgataata atatctcctt ccagttggtc tccttttgtt ttcatttcaa ttaattttaa
    13321 tgctgctgat aacactgtaa gggcaccatc tccatctgaa acaaaacctt taaccaacgg
    13381 acgagctcca ataccaccta gtctaccaat gattccgagc gtgggtgcat taccaccatt
    13441 ttttttgcca ttcttaccag ctatatatat ttttattcca tctgtataac catttttccc
    13501 ctgaatacta gtgacataaa cattctctgc tcctctttcc tttaaaaaat ctgccatctc
    13561 tgcaccgtta gaatttggct tatctaataa ctcaaatatt tctagtattt gttttaatat
    13621 cattaaatac cccctttctt taataggtat ctttctttaa agaataatac atatatagac
    13681 cagcactcat caatggatca ataacaggaa tcttaaattt ttttcttaaa atatctgaaa
    13741 caccgactgt agacatacca gtacaggata atgtaagcac atcaaaattg ctctcaaaat
    13801 tattaacttt ttcaatcaat ttattcaatt tgttcttatt aactaaatcc atactttctt
    13861 taatttcagg attatatata ataccgatta atttatcctt caaaatgtct ttataaacag
    13921 atggtatatc ttctaataat gacaaaacag caattttttc tccataaaat atagaagtaa
    13981 ctgatgctgc ccgaccagga ccaattacag gaatatcaag tttattatcg agttccttta
    14041 cagcaggatc ttctgcacag ctaataataa ttccatcaac ttcttgggaa atttttagtc
    14101 ctaaattaat gatcttagga acagctttat catgagctgc tttactattt attccttccg
    14161 gttggttttc aatacagaaa gttttagttt taaacatagg aaatgatctc tctatgattt
    14221 taccatgttt atattttatt tcttcatttt cagtgctaag tactcgtata atcccaacag
    14281 tatacttttt catttttttc accttcaaaa aatactattt gtccttatga tattattata
    14341 tagaaataaa tataattaat aaatatacat atcaaccaaa attttattgg attattgggc
    14401 ggactgcaca atatatatat cataataact gttttatctt taatgccata tataaattta
    14461 gataattttc tggattatct aaatcaacct ctaatatttt ttcaattttt ttaattcgat
    14521 aatttaaggt atttcgatga atacagagcc cttttgcagc agctgtttta ttaccgaagt
    14581 tttttattat actttcaact gtgtcaacca attcctcatt tttgggatta ttaactaatg
    14641 aacctatcac ttcttcatag aaatctatta aatcagggtc atcattcaat ttagctatta
    14701 ctctatatat acccaaatcg tcatatctag taattttatt ctggccataa atatttttgc
    14761 ccatactgag tgctttaaat gcttccttat aacttttctt taaaccatca atccctttat
    14821 ataaatttcc tatcccaatt gaaaccgtaa tatgtgaaat ctgttgcttg attgaacttt
    14881 taattttatg acaaaaattc tcaagctctt ttttatcttt taattcattc ttaactttat
    14941 tagatttata taaaactaca aatgagtcac tttgatttat aattataata tctttattta
    15001 acatcattaa ttcccattta ataattgatt ttatattttc ttttatcctt tgcataacat
    15061 cttcatcttt attttttaaa ctaagataat attcttcaaa gttatcaata tctatgacta
    15121 tgacatggta atcagacctt aaatcccagc caaaatattt tgctctttgg tatattgttt
    15181 tttcatcatt ataattatca tttatcaaat cctctattag ttcttctttt aatctatttt
    15241 gagtggcttc tttatctttt tctttaagca attctaatgc aatattagta gatgttaatt
    15301 caactgctct aaaatctata ttgtctaact tattattatc aatttcaaaa gcagaaacaa
    15361 aaccatatat tttgtctgta acaaaaattg gagctacaac tctcgcaata gtagcatctt
    15421 catatttaat tctcgtagcc tttctattga aagtaaaaag gtttagaagt tttttatcag
    15481 taatagatat attctttttt attttctttt cttcattata ttctccaatt aaagcaagcg
    15541 tttttaaatt tttatcttga atcaaaacag gtttttcgat taatttatgt aaagtttggg
    15601 ctaatagatc aataccacca ccctttaaaa tgacttttac caattcatta tgtatgtttt
    15661 caaatctact tatggaatta ttttttttac ctaatatttt actaaacaaa ggtaacatta
    15721 tactattgta atgaacgtct ttcgggatct taattatcgg cagattatat tcttcaccta
    15781 aattaattaa aaagtcggga attttctcta aatatcgtcc ttctttaata cagagagcgc
    15841 ctgctttaac ttctgacatt tttttaatca ttctctcttg taactctaaa tcgtctttta
    15901 tattataacc agtagttagg agaaaaacac cttctctaag ccattcatca atgtctggaa
    15961 cttccatgac atctacatac ttaacaaatc gttcgatgct gtctttacct gcaattattt
    16021 cagcatcctg catgaaatcc atgttaagaa tttctctcac tgtaagttcc ataataatct
    16081 tcctttctac aaattataat ataaatatat tctataaaac tttaaattgc aacattttaa
    16141 catatttttt attaaaatat tataattttt attgcacttc gaacaaatta aaaatacaat
    16201 ttaatcactt tttagtcaat agaaaatttg cacataaagt aaccttactt gaaaaattaa
    16261 ataattaaaa aaagcttcac cttttttgaa catttttaat aaaaaaatgt ccccaaagaa
    16321 aggtaaagct gataaatatt taataaatat ccttaaactt attattaaat taaatagcga
    16381 aaaaatagaa aataaaacta aaaaattacc cagtttaaag gctaaaatat taagttaatt
    16441 ctgttaattt cactttttat agcaaagtat caagtaagat aatcttaaat agatatttta
    16501 agaaattatc taaatcatat aacttaaaaa tttttattgc tttttaataa aaatttactc
    16561 gaatagatta gacacacgga tgatgagatt gtcaaattta aatttaatcc ttttttatag
    16621 acaaaacctt tttccagaat gataaacaat tctaactcaa cagattgagc tattatgtta
    16681 gattgaaata agaaataaaa tttagtaatt aaaacataat aaattgattc actcctatat
    16741 attagaataa aataaataat actttttctt tcttataaaa ctttaatatt tcccaattcc
    16801 tttaaagaat ataaatacaa ctttactgcc ctggctaata cactttcatc aaaatcaaaa
    16861 ttgtcttgat gatgcccggc cgcaagttca gttccaaagt taatataaca ggcttttccg
    16921 tcctgttttt gaactttatc catcaaaaaa ctggcatctt cggatccctg catactcttt
    16981 ttatctataa tattaccaat atcttcaata tcttgagcag cctcttttac tatattaatc
    17041 aattcattat cactttccat cgagatagct tcaccgacta cttctaattc taaattaaca
    17101 tcatatatct tttcgattcc ttttaatata tttttaactt tattatctag ataagagatt
    17161 acctcatcat tttctccccg aatttccatt tgaaggtatg cattagcagg tattagattc
    17221 ctgtaggttc ctgcttccat ttttcccaca tttactctag acataccttt tccgtgctgg
    17281 tttaaagaat ataaggaaga aattaggtta ttagctgcta aaagagcatt tttgcctttt
    17341 tccggagcac ccccggcatg agatggttca cctgtaatat taatatcaaa ttttttagta
    17401 taaagagaac cggtaattcc gcagataata ctattagaat catctgcatt catgccaata
    17461 tgacccgata aaaaataatc aacatcattt aataattcgc tttcagcaac tgctttacca
    17521 cctctaactc cttcttcagc tggttgaaaa attaatatta ttttaccttt caatttatct
    17581 ttggctgata gttcttttaa taatttagcc agaccaagtc caattgctat atgtccatca
    17641 tgaccacagc tgtggaccgc ctgtgggtta atagaacgaa agttctctct aacaggtctg
    17701 tgactatccg cattactttc ctcactgcca atagcatcga tatcaaacct aaaggcagta
    17761 ataggaccct ctttattggt atctaaaata gctgctgcac ccgtcatacc agcttttact
    17821 tcctctaata tttgaggttc tgtgccccaa tcaactgccc gcttgtacgc cttctcaaaa
    17881 tcttctatat taggtaaacc ttgtctgtac tctgtattta aaatatcttc tttatattta
    17941 acctcatatc ccagttcttt caaatatgtt attatttcag ccgtagtata aagctcaagc
    18001 cagccagttt caggatgact gtgaaatttt cttcttaaat tgattagctc tttttcaatc
    18061 gaattaacta aaaaatcaat atcaatcact aatacatccc ccttttccca ataaaaaatt
    18121 atcgcggaaa agatcattaa ctattaaaat aattatttct gttttactat ttctatcaaa
    18181 ctactatttc acctgcttta aagacagact ctacattaaa aatatcttca atgttttctg
    18241 caggatttcc cttgactacc accatatcag ccactttgcc ttcttcgata gaaccaaaat
    18301 catctaatat gtccagtaat tctgctcctc ctaaggtggc tgctttaatc gcttttatgg
    18361 gaggcattcc ggcttcaacc atcaacttta gttcataagt atttttgcca tgttcattaa
    18421 aaggggtgcc ggcatctgtg cccatagcta tcttaacatc atttttaaaa gcaaactcga
    18481 agctctcaaa atgttttttt atcacttttt tagatttttc cacagcatat tcaggaactc
    18541 cgttttccac acccttttca attatccaat aaggtgctac taaagttgca actaggaagg
    18601 tgcctctctc cttcatcata tcaacagctt cttggtccaa aaagaagcca tgttcgatcg
    18661 aatcaattcc cgctctaata gcgtttttta ttccaaccgt tccctgggcg tgggtagcag
    18721 tttttcgaca ggctttatga gcttcttcta cagctgcctg aatctcttca aaagaaagct
    18781 gtggagatcc cggctccaca ccttttgtca taactccccc ggtggacata attttaacta
    18841 catctacacc ggcttttaac tgttctctag cagcctttct agcatcagca actccatcgg
    18901 tttcccgtcc catacgatat ccatgtccac cggtcattgt aataactctt cccgcaacct
    18961 gcatccgggg tccattaaaa atacctctac ttgcagcctc tctcaatcct aaatcataat
    19021 gctctgcccc acctaaatcc cttacatagg tcactcccgc ttttaatgtt ttttccagag
    19081 ctttctgggc tttaatgatt gtaacagcat cattttcatt ttccattgtg gtatggggat
    19141 ctccggatgc atctaacata gaatgaatat gggcatcaat aatgcccggt aaaactgttt
    19201 tgccttctaa atcaataacc tcagcttctt taggtatttc aatatctcta cccacttttt
    19261 taaatttttc gttttcaact atgataacag catcttctat aactacttca ttttcaacat
    19321 caatataatc taaattttta tatgccttca aatttatctc tccttttcta ctctacttct
    19381 tattataaaa aaattaatat aaatgacagg ctacttctct accctcaatc ttttttaatt
    19441 ctggcttttc ccttaaacaa atatcctctg catacttaca gcgggaatga aaaaaacagc
    19501 ctcccggtaa ttctattgga tcaggtacat caccggataa aaccgaaaat tcttctttgg
    19561 tcttagttgt taattcggga atagagttaa ataatccttt tgtataagga tgaattggat
    19621 ttgaaaatat tttttcttta tcaccaatct caaaaacttt acccaaatac attatagcga
    19681 cccggtcact gatatgtttg acaacactca aatcatgggc gataaaaagg tatgttaaat
    19741 caaattcatc ctgtaaatcc atcagcaagt ttaatatttg agcttgaata gaaacatcta
    19801 aagcagagat cggttcatca gctacaataa actctggttc catggaaagg cttctggcaa
    19861 ttccaattct ctgcctttgt cccccactaa actggtgcgg gtaacggtta agataagaac
    19921 catccaatcc tactttatct aataaattta atatataatc atcttcctct tctctactca
    19981 caatattatg ggtccgaatt ggttctttaa ttatatcttt aaccttctgt cttggattca
    20041 gagaggaaaa aggatcctga aagatcatct gcattttttc tttcaaacca aacttctctt
    20101 ttgttgacat atcattaatt tttttaccat cataacttat ttcaccttcg gtgggttcat
    20161 aaagtcctaa aatagttctg ccgatagttg atttaccaca gccgctctcc cctactaaag
    20221 ataaggtctc tcctttctta atggtaaaag aaacaccgtt tactgcgtga acttgattgt
    20281 cattattaaa caaatttgcc caaaatccat catttacgga aaaatgtttt tttaaatgct
    20341 ctacttgcag aagttccata tcagcccact cctgctatca aatcattttt aatttggcgg
    20401 gcttcactac aggtagcaaa atgatttttc tcaatttcaa tcagttcatt attataattc
    20461 ctacaacggt ggcttttcct gctacatctt gaatggaatt tacatccgtc cggcaaatcc
    20521 agtgggctgg gcattaaacc tttaatttgc tctaatcttt tttggtctct atccatcatg
    20581 ggaatagatt tgattaaacc ttttgtatag ggatgaacgg gattgctaat tatcttgctt
    20641 tttcgtccat gttctataac atctccggca tacataacca taatacgatc tgcaatctca
    20701 gaaaccaaag ccaaatcatg tgtgataaat attaaagaag tttgtctgtc cttgattaga
    20761 tttttcataa gccctattat ctgggcctga atcgttacat caagggctgt tgtcggttca
    20821 tcagcaataa taatttcggg gtcactggcc agggcgatgg caatcacaac tcgttgtctc
    20881 atccccccac ttaactgatt agggtaatgt ttaagtcttg tttcaggtga tgatatgcct
    20941 accatttcca gtagctcaat agctctattt ttagcttctt ttcgactgta atttaaatgg
    21001 aactgtaata cctccattaa ctgccgtccg atagttaaaa cgggatttag tgaagccatg
    21061 ggatcctgga aaatcataga aattttttcc ccgcggatct tattcatcct ttttttattc
    21121 atctttaata aatctgtatc tttatatttt atctctccct caactatctc gcccggggaa
    21181 tctattagtc taagtaagga aaaaccagta accgatttac cggcccccga ttctcctact
    21241 attcccaaaa tctcaccttt atggaggtta aaactaaccc cattgacagc tttaacaact
    21301 ccctctttgg tattaaaatg tgttcttaaa ttattaactt caagtatttt ttctttctgc
    21361 atatttactc ctcctctttt ctctactctt atatacagaa tttgttattt taacttagga
    21421 tttaaaacat ctcttaacca atcaccaata atattgatac ttaatacgat catcactaag
    21481 aagccgcccg gaaatactac caaccaccat ttaccactca atagccgttt ataaccatct
    21541 gcgatcataa gtcccaggga aggttcggtt aatggtactc cgatacctaa aaaactgagt
    21601 gtagcctcaa gcataatgac ctgggctatt tggacagtag cgatgacaat gataggggtt
    21661 aatatgttgg gtaaaatatg tcttaataaa attttagtat ttgtaacccc aattaatttg
    21721 gctgcttcca caaattcact actttttatc gaaagtgttg agccccgggc agttcttgca
    21781 taggtaaccc aggtagcaag accaattaca actatcagtt taagaatacc acgtcctaaa
    21841 aaagccatta ctgtaatggc aattaacatg gttggaaaag acagctgtat atctgccatt
    21901 ctcattatta tactatctac tttcccacca aaatagccgg caatcattcc taaggtaata
    21961 ccaattgttc ctcccagtgc tacagcagca aatcctacta acaaggatat ccttgtacca
    22021 tataaaatgg tgcttaagat acctcttccc tgtaagtcag aacctaatat gaatttatca
    22081 actcctcctt ccatccagac cggtggtttc attgaatcca ttagagatac tgatgcaagg
    22141 tcatatggat tatgaggtgc aataatatgg gcaaaaatac tggcagcaat aattaataca
    22201 agaatgatag tcgcaataat aacaagcttg tctcttttaa agtaataaat aagatcttcc
    22261 atgtatatat tacctcctta acccaattca atgcgggggt cgaaaaaata atacagtaaa
    22321 tcgacaatta agtttatagc tgcaaacaag gcagcaacta ccaggatata cgacataata
    22381 actggacgg
//
LOCUS       DATGCJ010000073         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_410741, whole genome shotgun sequence.
ACCESSION   DATGCJ010000073 DATGCJ010000000
VERSION     DATGCJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410741"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /gene="istA"
                     /locus_tag="VKN64_03955"
     CDS             complement(<1..1317)
                     /gene="istA"
                     /locus_tag="VKN64_03955"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HMA59426.1"
                     /translation="MLKMEEIARIKKYRSQGKSKRAICRETGHAWDTVTKYIEQKDFN
                     IYPKKRKPRKTKLDGYRKIIDKWLLEDQDMPRKQRHTATRVHARLQEKYGDDFAASYR
                     TVAYYVKEARARIDQESKACFLDLDHKPGEAQVDFGQANFLEKNKKIKGHYLVMSFPY
                     SNAEYCQVFKGENQECFLEGMKRIFERIGRVPKEIWFDNLSAAVTIKKGQRKMTDRFK
                     RFMLHYGFEAHFCNVNKGNEKGHVENKVGYVRRNLFVPMPGFKDIDEYNGKLLDQCEE
                     HMKEEHYKKEVEIKQLFQTDLENMKLINTVPFDTSKVINIVTDNYGYFRLSDRTYSTG
                     GYYKTSELLARVTHNQVIVMDQNYDIIVKHPRLYGDQQESILWQPYLKLIAKRPNALK
                     YVKFYDTLPYNWQAYLSGLKRDDKKEALRILSEILLEHDLDIAEQAL"
     gene            1669..2220
                     /locus_tag="VKN64_03960"
     CDS             1669..2220
                     /locus_tag="VKN64_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59427.1"
                     /translation="MKKLIIIMLLTSYLISIDAKTLDSPKYYLKTNLGVCTESNILGP
                     ALINEFGSLIDNNLYFTSRLVLSTSFGDDINKGAHFPNPKINYSYYSLGFNLIYSFFP
                     DARFKTGCGIVYGTTKRIEYKEYSNSKALSNYSAIIDPSLGVSLSLDYQFFNTDHFNI
                     GTDIIGQFGSRRVYLIGLYFRMK"
BASE COUNT          886 a          467 c          423 g          996 t
ORIGIN      
        1 aagggcctgc tcagctatat caagatcatg ctcaagcaat atttctgata gtatgcgcag
       61 agcttctttc ttatcatctc gcttaagacc tgataaatat gcttgccaat tgtaaggaag
      121 tgtatcataa aattttacat actttaaagc attaggtcgt ttggctatta acttcagata
      181 gggctgccac aggatagact cttgctgatc accataaagt ctgggatgct taacaataat
      241 atcataattc tgatccatta caattacctg attatgagtg accctggcca acaattcact
      301 tgttttataa tatcctcctg ttgaataagt tctatcagag agtctgaaat aaccataatt
      361 atccgtaact atattaataa cttttgaggt atcaaaaggc accgtattaa tcagtttcat
      421 attttccaga tcagtctgaa atagctgctt aatttcaact tcctttttat aatgttcttc
      481 cttcatatgt tcttcacatt ggtctaacaa tttcccgtta tattcatcaa tatctttaaa
      541 tccaggcata ggtacaaata aattccttct aacataacca actttatttt caacatgacc
      601 tttttcatta cctttattaa cattgcaaaa atgggcttca aaaccgtagt gcaacataaa
      661 tcttttgaaa cggtctgtca ttttacgctg gccctttttg atagtaactg ctgctgataa
      721 attatcaaac catatctctt ttggaactcg cccaattctt tcaaatattc ttttcatacc
      781 ttccagaaag cactcctgat tttcaccttt aaaaacttga cagtattctg cattactata
      841 gggaaaggac attactaagt agtgaccctt tatcttcttg ttcttctcaa gaaaattagc
      901 ctgtccaaaa tctacctgcg cttcacctgg cttatgatct aaatcaagaa agcaggcttt
      961 actctcttga tcaattcttg ctctggcttc ctttacataa taggcaacag ttcgataact
     1021 agcagcaaaa tcatctccgt atttttcttg aagcctggca tgtactcgag tggcagtatg
     1081 acgttgcttg cggggcatgt cttgatcttc taataaccat ttgtcgatta tttttctata
     1141 accatccaat ttagtctttc ttggtttgcg tttcttggga tatatattga aatccttctg
     1201 ctcaatatat ttagtaaccg tatcccaggc atgaccggtt tccctacaaa ttgcccgttt
     1261 tgacttccct tgtgatctat actttttgat tcttgcaatc tcttccattt ttagcatcct
     1321 ttctcttacc ctccatgttt atttttatgg aggtaattta gttaggagct aggattttat
     1381 cttgcaaatt atgacctctt ctatgcaaag agctctattt ttatcttgca atctgctagg
     1441 tttctaattt gcaaaaaaca aatggcagag caaatctaaa aatatattca acttattatt
     1501 aatctaaaaa aatatcataa tttacatata gactattact cggtagttaa caaagcaagg
     1561 acaatatagt catagtaaaa tgatggtagc tttatttgat ttaagcatct taaatataat
     1621 atttattaaa tttagataat ctgaaaattg taattgaaag gctctaacat gaaaaaatta
     1681 attattatta tgttattaac atcctattta attagtattg atgctaaaac tctggatagc
     1741 cctaaatatt atttaaaaac aaatcttgga gtttgtacag aaagtaatat tcttggacca
     1801 gctttgataa atgaatttgg ttctttaatc gataataatt tatactttac atctcgtttg
     1861 gtactatcta ctagttttgg agatgatatt aataaaggtg cgcatttccc aaatccgaaa
     1921 attaattatt cttattattc tctggggttt aatttaattt attcattctt cccggatgct
     1981 cgatttaaaa cagggtgtgg aattgtttat ggaactacaa aaaggattga gtataaagaa
     2041 tatagtaatt caaaagcatt atccaattat tctgcaataa tcgatccttc actaggagtg
     2101 agcttgtctt tagattatca attctttaat acagatcatt ttaatatcgg aactgacatt
     2161 atcggacaat tcggaagtag aagggtatat ttaattgggt tatattttcg tatgaaatga
     2221 tttctataca gagcattcac gctattttta ttatttttga ttaattgttt cagcgctgaa
     2281 ttattatttt ataaacaaag ggatttctta tggtcaggcc gagaatcaaa ttctataatc
     2341 aaagtttgtg actaaaatat tgatcttcca aaagaaaaaa gattatgaaa attgtgagat
     2401 taaattgaaa ttaacagatt tgatttttaa actggaaaag tttcagattt ataaaacaag
     2461 tgcatgtgat tgaaaggtgc tatcgaacta aaaatctaaa gtctctaacg gaagttgcac
     2521 cgaacatcga aagctggagg attttcggag ttgggggagt ttttgagcaa tatttttgcc
     2581 attttcgaac gattatagta aaattgatac gatgtcggtg aaactaagct tgttatattt
     2641 aaataagcta cggtaataaa aacattaata atggaggaaa aattaatatt tacaatgaat
     2701 aagcccggaa tagtatcgtt attctactcc gggcggccaa tcgtcaatct accagaaaaa
     2761 caattggtaa tt
//
LOCUS       DATGCJ010000074         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_322322, whole genome shotgun sequence.
ACCESSION   DATGCJ010000074 DATGCJ010000000
VERSION     DATGCJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322322"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="VKN64_03965"
     CDS             complement(<1..2232)
                     /locus_tag="VKN64_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HMA59428.1"
                     /translation="MTILYNSGSNEYKNWIIEETEFKKENQANFETVFSLGNGYMGLR
                     ASTEESYPGETRGFYIAGVFDEFPGEVTEMPNLPDWIKTQIYVNGKKFKLDIGKTYEY
                     SRCFNLKDGLLSRSFIWESPQGEMIEFYFERFISMNNLHTGVLKIELKPLNFSGEIKI
                     ISGFNGRISNSGTQHLKEGEKRLIDDYIVYKPETQESEINMSLGLNHEFSIDGENIKP
                     TKDFKADRRQIFKTYEFEVREKEKVTLIKRLSAYTNQDKDVKNEENLEEKMIDNLIDN
                     RKTDYQILKDHHLKKWHSIWEQIDVKISGSDYDQLALRFALFHLIQMTPDHDDRISIA
                     AKGLSGEGYKGHVFWDTEVFILPFFIYNKPETAKKLLKYRYNTLEGARQKATQNGYEG
                     AMYAWESARTGEETTPKYGAVDILTGEEIRIWCGEIEQHITVDIQYAFNKYYKVTNDI
                     DFMKSYGFEVFFESARFWASRLEYNQAKDQYEINNVMGPDEYKEHIDNNAFINYMVNW
                     QFNQALEYIRFLNENHKQDLESLKERIDLKDNEIKNWAELKDKIYLPQPGEDNIIPQF
                     EGFENLDEIDLSKYKKGDVGDIFNDLGWEEIINSKVSKQADLVMLTYLLEDKFKEEII
                     RANFDYYEPLTLHDSSLSPGIHAAVAGKLGEIKKAYELFKRAAQIDLGPNMDSSKAGL
                     HSASLGGLWQATIFGFGGLKFEDNILKIKPNLPERWNMLSFKLSFKGNKVKIEINSEV
                     IKID"
     gene            complement(2296..2371)
                     /locus_tag="VKN64_03970"
     tRNA            complement(2296..2371)
                     /locus_tag="VKN64_03970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2336..2338),aa:Gly,seq:gcc)
     gene            complement(2389..2473)
                     /locus_tag="VKN64_03975"
     tRNA            complement(2389..2473)
                     /locus_tag="VKN64_03975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Tyr,seq:gta)
     gene            complement(2636..4306)
                     /locus_tag="VKN64_03980"
     CDS             complement(2636..4306)
                     /locus_tag="VKN64_03980"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA59429.1"
                     /translation="MKSDIQIAQEAEMKPIIDIAEQLGLSKDDLELYGNYKAKLKLDV
                     FKERKDKKDSKLVLVTAITPTPAGEGKTTTTVGLGQALNQRDKKAMIAIREPSLGPTM
                     GVKGGAAGGGYSQVIPMEDINLHFTGDIHAIGIAHNLLSAAIDNHIKQGNELGIDPTE
                     IGWPRVVDMNDRALREVVVGLGGSANGKPREDGYIITVASEIMAILCLANDLMELKEK
                     IGNIVIGYNYEGDPVTAGDLNVQGAMTALLKDAIKPNLVQTLENTPALIHGGPFANIA
                     HGANSVMATKMAMKLSDITITEAGFGADLGAEKFFNIKSRYANLNPDATVLVATIRAL
                     KMHGGVDKDNLEEENLEALAKGMENLEKHVENISKFNVPLVVGINRFPSDTEAELELV
                     REKCEELGVRVALSEVWAKGGEGGLELADEIIDILENEESNFEYLYSKELSIKEKIEK
                     IAEEIYGADGVDYSSTAEKQIANFESQGYGELPICMAKTQNSISDKPDLKGWPTDFTV
                     SVSKVTLSAGAGFLVPSTGPVLKMPGLPKRPSAEDIDIDEDGKITGLF"
BASE COUNT         1290 a          945 c          671 g         1576 t
ORIGIN      
        1 atctattttg attacctcac tatttatttc aatctttact ttgtttcctt taaaagaaag
       61 cttaaagctc aacatattcc accgttctgg taagttgggc ttgattttaa gtatattatc
      121 ttcgaatttg agacctccaa atccaaaaat tgtagcctgc cataatccac ccagtgaagc
      181 tgagtgaaga ccggctttac tgctatccat attcgggcct aaatcaattt gggcagctct
      241 tttaaagagt tcatacgcct ttttgatttc acctaattta ccagcaactg cagcatgaat
      301 accagggctt aaagacgaat catgtaaagt aagtggttca tagtaatcaa agttagctct
      361 aataatctct tctttaaatt tatcttctaa taaataggtt aacattacta aatcagcctg
      421 tttagatacc tttgaattaa taatttcttc ccagcctaga tcattaaata tatcgcctac
      481 atcacccttc ttatatttac tgagatcgat ctcatctaag ttttcaaaac cttcaaactg
      541 gggtataata ttatcttctc ctggttgtgg gagataaatt ttatctttta gctcagccca
      601 gtttttgatt tcattatctt tcaaatcaat tctttccttt aaactctcta agtcttgctt
      661 atggttctca tttaaaaatc taatgtattc taaggcctga ttaaactgcc agttgaccat
      721 ataatttata aaagcattgt tatcaatatg ttccttatac tcatcaggtc ccataacatt
      781 atttatttca tattggtctt tggcctgatt atattcaagt cgtgaagccc aaaagcgagc
      841 actttcaaaa aacacctcaa atccatagct tttcataaaa tctatatcat tggttacttt
      901 ataatattta ttaaaagcat attgaatatc tacagttata tgctgttcaa tttctccaca
      961 ccaaatccta atctcttcac cagttaaaat atccacagca ccatacttgg gagtggtctc
     1021 ttcaccagtg cgggcactct cccaggcata catagcccct tcatagccat tttgagtcgc
     1081 tttttggcga gcaccttcta aagtattata tcgatatttt aagagctttt tggcagtctc
     1141 aggcttatta tagataaaga aaggtaggat aaatacttca gtatcccaaa agacatgtcc
     1201 tttatatcct tcaccactta aaccttttgc tgcaatacta attctatcat catgatctgg
     1261 tgtcatctga ataagatgaa aaagagcaaa cctaagggcc agttgatcat aatcactgcc
     1321 cgagattttt acatcgattt gttcccatat ggaatgccat tttttcaggt gatgatcttt
     1381 aagaatttga taatcggttt tccgattatc tataagatta tcaatcatct tttcctctaa
     1441 attttcttca ttttttacat ctttatcttg gtttgtataa gcacttaatc ttttgattaa
     1501 ggtcaccttt tctttttctc taacttcaaa ttcatatgtt ttaaaaatct gtctgcgatc
     1561 agctttgaaa tctttagtgg gcttaatatt ctcgccatct attgaaaact catgatttaa
     1621 tcccaagctc atattaattt cagattcctg tgtttcaggt ttatatacta tataatcatc
     1681 aattaatctt ttttcacctt cttttaagtg ttgggtacca ctgttactaa tccgtccatt
     1741 aaaaccagaa atgattttta tttcacctga aaaatttaac ggttttagtt ctatctttaa
     1801 aaccccagta tgcaaattat tcatcgaaat aaatctctca aagtaaaatt caatcatttc
     1861 accctgtgga ctttcccaaa tgaaactacg agaaagtaaa ccatctttta aattgaaaca
     1921 acggctgtac tcataggttt tacctatatc taacttaaat ttttttccat tgacatatat
     1981 ttgtgttttt atccaatcgg gaaggttcgg catttcagta acttcaccag gaaactcatc
     2041 gaaaacacct gcgatataaa atccacgagt ttcacctgga taagactctt cagtgctagc
     2101 ccgaagtccc atatagccat tacctaaact gaaaaccgtt tcaaagttag cctgattttc
     2161 ttttttaaat tctgtttctt caataatcca gtttttatac tcattacttc cactgttata
     2221 taatatagtc atagaagcca cctcaaatca cataatcaaa tattttcaat tttcacacta
     2281 tttaatatat aaaaatggag cggaaaacgg gactcgaacc cgcgaccttc tcgttggcaa
     2341 cgagatgctc taccagctga gctatttccg cttggtatgg tgtgataatg gtggagggga
     2401 gaggatttga acctctgtag gcatcgccgg cagatttaca gtctgctccc tttagccact
     2461 cgggcacccc tccatgtatt taattaaaga acttaaaacg ccttaaatat taatatatca
     2521 acgtttcaat ccttatttat agtattatat tttgggctat ttgtcaagag taaaatctaa
     2581 attgataatt taagagttgg ggttaagtgt cttaacttaa ccccacctcc tttatttaga
     2641 acagcccagt aatcttgcca tcttcatcaa tatcaatatc ctcagcagaa ggtctcttag
     2701 gtaaaccggg catcttaagt accggtcctg tggaaggtac taaaaagcct gctccagctg
     2761 aaagggttac cttagatact gaaactgtaa agtcagtcgg ccaccccttg agatctggtt
     2821 tgtctgaaat agagttctga gttttagcca tacagattgg tagttctcca taaccctgac
     2881 tttcaaagtt cgcaatctgt ttttctgctg ttgaactgta atctactcca tctgctccat
     2941 aaatctcttc cgctatcttt tcaatctttt ctttaattga aagctcttta ctgtataaat
     3001 actcaaagtt gctttcttca ttttctaata tatctataat ctcatcagct aactcaaggc
     3061 cgccttcgcc acctttagcc catacttcag atagggctac tctaacaccg agctcttcac
     3121 atttttctcg cactaactct aactcagctt cggtatcaga agggaatcta ttaattccta
     3181 ctacaagggg aacattgaac ttactgatat tctctacatg tttctctaag ttttccatac
     3241 ctttagctaa ggcttctaag ttctcttctt ctaagttatc tttatcaaca ccaccatgca
     3301 tcttcaaggc tctaatcgta gccactaata cggtagcatc tggattaaga tttgcataac
     3361 gggatttaat attaaagaac ttctctgccc ctaagtcagc tccaaagccc gcttcagtta
     3421 tcgtaatatc agatagcttc atcgccatct tggttgccat tacactgtta gcaccatggg
     3481 caatgttagc aaatggtcca ccgtgaatta aggcaggtgt gttctctaaa gtctgtacta
     3541 agttcggctt gatcgcatct ttaagtaatg cggtcattgc cccttgaaca tttagatctc
     3601 cggctgtaac aggatcacct tcataattat agccgattac aatatttccg atcttttctt
     3661 taagttccat tagatcattg gccagacata agatagccat gatttcagaa gctacagtta
     3721 tgatatagcc gtcttctctg ggcttaccat tggctgaacc acccaagccg actactactt
     3781 ctcttaaggc tctatcgttc atatcaacca ctctaggcca gccgatctcg gttggatcaa
     3841 tacctaattc attaccttgt ttgatgtgat tatcaattgc agctgaaagt aggttgtgag
     3901 caataccgat tgcatggata tcacctgtaa agtgtaggtt gatatcttcc atgggaatta
     3961 cctgggaata gccgccaccg gctgctccac ccttaacacc catggtgggt cctaaggaag
     4021 gttctcttat ggcgatcatc gcctttttat ctctttgatt taaagcctga cccagtccaa
     4081 cggttgtagt agttttacct tcaccggctg gagtaggagt aatagctgta accaagacta
     4141 gtttactatc ttttttatct tttctttctt taaagacatc tagttttaac ttggctttgt
     4201 aattaccata tagttctagg tcgtctttgc ttaatcctaa ctgttcggca atgtcaatga
     4261 ttggtttcat ttctgcttct tgtgctattt gaatatcact tttcaaaata tcattccttt
     4321 ctatttatgg tttaataaaa attattaaaa attaataaat gccctgataa tttttttgta
     4381 ctacagcaga atatacataa tttatcattg taatataatc aaaaacaggt aaatttgttg
     4441 ctttttgtac cgcatttgaa taaggaggca aaacactaca tt
//
LOCUS       DATGCJ010000075         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_546308, whole genome shotgun sequence.
ACCESSION   DATGCJ010000075 DATGCJ010000000
VERSION     DATGCJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546308"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VKN64_03985"
     CDS             complement(<1..280)
                     /locus_tag="VKN64_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMA59430.1"
                     /translation="MYKYLTQGLLLGLAYVAPIGMQNLYVINTALSGDKKRTYQVAGF
                     TIIFDILLALACFFGIGLLLKNVPSLKSTILLVGSILVILIGFKLIKEA"
     gene            complement(323..919)
                     /locus_tag="VKN64_03990"
     CDS             complement(323..919)
                     /locus_tag="VKN64_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA59431.1"
                     /translation="MILDIIKQRKSVRKFSDKELSEQKINYILEAGRLSLSGGNEQPW
                     KFGVVKDKSLIKKTAKIAYNQKWIEGAQALIVLCTRIATDREGGRDIQLSRYTEWRTE
                     IETMDKKLYSKLNLEEHQTKIPGTHMVLAATEQGIGSIWISYFKVTELADLLGLPENY
                     LPSEIIAFVYPKKERKRTTKKSLEEVVFFDDDFYNQNN"
     gene            complement(936..1574)
                     /locus_tag="VKN64_03995"
     CDS             complement(936..1574)
                     /locus_tag="VKN64_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59432.1"
                     /translation="MDKKINNIINNTYFIGGSPCSGKSTTAEILSNKYDLKYYKIDDY
                     EFAHLERAEKEKHPTMSKYKQMNWDEIWMRDPKLQANEEFQFYRERFEFVLDGLKKYN
                     ENDKIITEGAALLPELLNELNIDKNRISYMIPEKEFQIKHYTKRTFKDHILKECSQPK
                     KAFENWMERDHIFAQKVKGQAKKFGYDLLEIDGSNTIESNLNIIENHFNLTK"
     gene            complement(1673..>2069)
                     /locus_tag="VKN64_04000"
     CDS             complement(1673..>2069)
                     /locus_tag="VKN64_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59433.1"
                     /translation="NFDLIILACNCLYELATPEQQAKIIQNAYDSLNEGGFIFIDNDN
                     MEGKLDKSWCEIGKEKKIFPNGTCRHGIEFKGAYKNIYVDRAKRIWQAERKVEINYPD
                     GQKEVKKLKQQKHPVSAQEIKKWLVENNF"
BASE COUNT          603 a          384 c          265 g          817 t
ORIGIN      
        1 ccgcttcttt aattaattta aatccgatta agataactaa aatagagcca actaataaga
       61 tggtgctttt tagggaaggg acatttttaa gtaaaagacc aatcccaaaa aaacaggcta
      121 atgctaaaag gatatcaaaa atgatcgtaa aaccggctac ttgataagtc cttttcttat
      181 caccacttaa agctgtattt atgacataga gattctgcat accgatcggc gccacatatg
      241 ctaaacctaa taataagccc tgtgttagat atttgtacat tattaactct ccaattatta
      301 atatttagtt gctattatct tattaattat tttgattata aaaatcatca tcaaaaaata
      361 ctacctcttc taagcttttc ttggttgttc tttttctttc ttttttagga taaacaaaag
      421 caattatttc agacggcaga taattttccg gtaagcctaa taaatcagct aattctgtca
      481 ccttaaaata tgaaatccag atcgaaccaa taccttgttc agtggctgcc agcaccatat
      541 gagttccggg tattttagtt tggtgttctt ctaaattcaa tttagaataa agtttcttat
      601 ccatcgtctc aatttcagtt ctccattcgg tatacctgct taattgaata tcacggccgc
      661 cttctcggtc ggttgctatc ctagtacaca gtacaattaa agcctgcgca ccttcaatcc
      721 atttttgatt ataggctatc tttgctgttt ttttgattaa acttttatct ttgacaactc
      781 caaacttcca gggctgttca ttgcccccag agagagataa acggcccgct tctaagatat
      841 aattaatctt ttgctcagaa agttctttat cagagaattt tcgtacactt tttcgttgct
      901 ttattatgtc taaaatcata aattaccact ccaaattatt ttgtaagatt aaaatgattt
      961 tcaattatat taagattgct ttcaatggta ttacttccat cgatttctaa taaatcataa
     1021 ccaaattttt ttgcctgtcc ttttaccttt tgggcaaata tatggtctct ttccatccaa
     1081 ttttcaaatg cttttttagg ttgcgaacat tcttttaaaa tatgatcttt aaaagttctt
     1141 tttgtataat gtttaatctg aaattctttt tcaggaatca tatatgatat tctgttttta
     1201 tctatattta attcatttaa tagttctggc aagagggctg ctccttcggt aattatttta
     1261 tcattctcat tatatttttt taatccatct aaaacaaatt caaatctttc ccgataaaat
     1321 tgaaactctt cgtttgcttg cagtttggga tcacgcatcc aaatttcatc ccagttcatc
     1381 tgtttatatt tagacatggt ggggtgtttc tctttttcag ccctttcaag atgtgcaaac
     1441 tcatagtcat cgatcttata gtactttaaa tcatatttat tacttaatat ctctgcagta
     1501 gtactctttc cactacaggg agaaccaccg ataaaatagg tgttattaat tatattattt
     1561 atctttttat ccacttacct caccttttct cttttttagc tataaaggta gctcttttag
     1621 cacctttaaa aaattcctta ttctgtttaa gatctgaata cattttaatg atttaaaagt
     1681 tgttttctac taaccacttt ttaatttctt gagctgagac aggatgtttt tgttgtttta
     1741 atttctttac ttctttttga ccatctggat aattgatttc tacttttctt tcagcctgcc
     1801 agatcctctt tgctctatct acatatatat ttttatatgc ccccttaaat tcaatcccat
     1861 gacgacaggt tccatttgga aagatctttt tctctttgcc tatttcacac caggatttat
     1921 ctaattttcc ttccatatta tcattatcta taaaaatgaa accaccttca tttaaagaat
     1981 cataagcatt ttgaattatt ttagcctgct gttctggtgt ggctaattca taaagacagt
     2041 tgcaggctaa aattattaga tcaaaattt
//
LOCUS       DATGCJ010000076         8522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_367112, whole genome shotgun sequence.
ACCESSION   DATGCJ010000076 DATGCJ010000000
VERSION     DATGCJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367112"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VKN64_04005"
     CDS             <1..112
                     /locus_tag="VKN64_04005"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HMA59434.1"
                     /translation="VLGSSVKEDGSIIMENNSMFCGDQIAGWSPKTMKEF"
     gene            131..1450
                     /gene="grdH"
                     /locus_tag="VKN64_04010"
     CDS             131..1450
                     /gene="grdH"
                     /locus_tag="VKN64_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1181..1183,aa:Sec)
                     /transl_table=11
                     /product="betaine reductase selenoprotein B"
                     /protein_id="HMA59435.1"
                     /translation="MPKAIVYLNQFFGQIGGEEVADHEPEIREEKVGAAMLYDQLLDG
                     EVSHTVICGDNFFGSNEEEAIDRILGFLEDKEFDIFFAGPAFQAGRYGTACGAICKAV
                     KEEFDVPVISSMNEENPGVDMFKKDIYIFPGGQSAAKMKEDVTKMVEFGNKVLSGEKL
                     LPAEQEGYFTRGIRHQFFLPDETPAYERAIDMLLKKINNEEFESELPIPELDTVDIAD
                     PIEDLSEATVALVTSGGIVPIENPDNIQSASATRWGKYYIEDVNRLKNRRSEAFRTIH
                     AGYDPAAADTDPNRVVPVDAMRKYEREGKIGELYKYFYSTVGTGTTQGEAARMGQEIA
                     KELLEDGVQAVILTSTUGTCTRCGATMVKEIERAGIPVVQMANLIPVAKTVGANRIVP
                     TISIPYPLGDPSTSEDEQWKLRYHRVGVALDALTTDIDEQTVFDVEI"
     gene            1553..3133
                     /locus_tag="VKN64_04015"
     CDS             1553..3133
                     /locus_tag="VKN64_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HMA59436.1"
                     /translation="MKTRKDNRGNVIKERGKEVFLISLIVVFAIVIWGIAMPENFSNV
                     ANSLFDYLTGNFGWFYLITMSLFVIFCIWMALSKHGKIRLGKPDEEPEFSTISWFAML
                     FSAGMGVGLVFWGVAEPLNHFVNPLNMQGGTAQAADFALKTSFFHWGLHPWASYGVLA
                     LALAYMQFRHDKPAQISSIFIPLIGEAKARGTIGKGIDILAIFATVAGVATSLGMATL
                     QVTSGFSFLFGIPETNLVRLLVIGVITILFMISAITGVDKGIKYLSNVNISLAGLIMV
                     ICLIVGPTVLIINNFTNSFGIYMSSIVRDSFKIGKEPWYGWWTIFYWAWWIAWAPFVS
                     TFIARISRGRTIREFIGGVLFAPTLASFVWFSILGTMGIESGMNVAEEAIEVTETAFF
                     VVMQNFPFGGLISIIAVILLTTFFITSADSATFVLGMLSDKGNQNPTAKRKVTWGFIQ
                     SGLAIALMLSGGLGMLQTGSIVAAFPFAIIMIFAMVAMIKSVKAEHKVEGVADLNKVA
                     DTSLEDLKEADELDEAVN"
     gene            complement(3223..3894)
                     /locus_tag="VKN64_04020"
     CDS             complement(3223..3894)
                     /locus_tag="VKN64_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59437.1"
                     /translation="MIDINNKDIKKKRILKYFIQATKEVIEEKGIENVTIRNVAKRAG
                     YNSATIYNYFDNYKQLIFFASLDFLSNYIKAMPDYIAEGEDEVEKFILMWECFCKYSF
                     ETPQIYYAIFTEELGDHPESLMEKYFQIFPEKLGDPPERLVPMLLDPDLSRRASIASK
                     PLIENDYLSKEKAEEVDNMITYIYHGMLTLIINNRIDYSVDEAVNKITGHIRTIVNNA
                     ICCDK"
     gene            4071..5276
                     /gene="trxB"
                     /locus_tag="VKN64_04025"
     CDS             4071..5276
                     /gene="trxB"
                     /locus_tag="VKN64_04025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMA59438.1"
                     /translation="MSNTYDLIIVGGGPAGMSAAIYGSRSRLDTLVLEAKRKTGGQPA
                     TYHDMENYPGVPETTADELMDNFRNHAEKFDTEFKRGEVTDITVNGFEKVVKTKKGEE
                     YKAKSVVLAMGAEPRKLGIKGEEELKGKGVSYCATCDADIFEGLDVVVIGNGNSAVEE
                     SIYLTKFVNKLTMVVIHDEGVMDAEKILQEKAMENDKIDFVWNSVVEEIKGEEMVEGV
                     VLKNIKTGEKSELDCHGVFFFVGRVPNTDFLEGTVELTEHGYIKTNDEMETNQPGVYG
                     VGDVRNKVVRQVITAAGDGATAAVMAQGYIEEEEYWQNNVVESDEPVLVGFWSVTHQD
                     SMDMMSEIECMNLDEVDCRFVKIDTYKNRLISNRYDIDEIPTVVKIEDGEVKAKLVEP
                     TVDEVQELL"
     gene            5298..5615
                     /locus_tag="VKN64_04030"
     CDS             5298..5615
                     /locus_tag="VKN64_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMA59439.1"
                     /translation="MLELGKENFEDEVLNAEGVVLVDFWSENCDLCLDLMPDVEELAE
                     EYGDQIKFAKLDIKGNRRLAIGQKVLGLPSIVLYEDGEKVEHLSGDELEVEDIREAIE
                     ERL"
     gene            5692..7233
                     /gene="grdC"
                     /locus_tag="VKN64_04035"
     CDS             5692..7233
                     /gene="grdC"
                     /locus_tag="VKN64_04035"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HMA59440.1"
                     /translation="MSFPVIKGSSYILVHTPNILKQGGSTQTTTRAKNPNDEYLNKMD
                     EHLRSFEEFVGYAPNQAYIGNILPNDLEDIETPWYDSDNLLEAKKEGEFGEIMSEEVF
                     IPLVKHVDVFDLVKLTKEFSKRGQELVNEHPILKDMDIDLGEGEELSSVEELVEAHAE
                     PFYLDGKLVGCVKSAHETDINLSAHVIYENLIGKASAVLAMKNLGYRNNIDLSEVDYI
                     LECSEEACGDINQRGGGNFAKAIGEMAGATNATGSDVRSFCAAPAHAMVNAAALVKSG
                     VYDNVAVVAGGSVAKLGMNGKDHVKKDMPILEDGLGGFAILISENDGENPVLRTDIVG
                     RHRVGTGSSPQAVISSLVTDPLDEMDISIKDIDKYSVEMQNPEITKPAGAGNVPESNY
                     KMIAALGVKRGDLERKELMNFVEEHGMPGFAPTQGHIPSGVPFVGPAVKMIEDGDIEN
                     AMIIGKGSLFLGRMTNLFDGVSFVIENNEGLQEKEASVSDEEIKSMIADAMRKMAENM
                     TVESN"
     gene            7244..8383
                     /gene="grdD"
                     /locus_tag="VKN64_04040"
     CDS             7244..8383
                     /gene="grdD"
                     /locus_tag="VKN64_04040"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HMA59441.1"
                     /translation="MADKTKEKVAGIFNELADAIESGQFGEKKKVGLTLLDSEHGPEE
                     LQKAAEMAEKNNSDIEVETIGCDGSKCSCLADAHEAMDNMLDSGEIDASVTLHYNFPF
                     GVSTVGRVITPGRGKEMLIATTTGTSDTDRVASMLKNAVYGIATAKSLGIKEPKLGIL
                     NVDGARLVENNLRELKDNGYDIQFATSVRSDGGVVMRGNDLLMGVPDVMVTDTLTGNM
                     LMKIFSAYTTGGKYEASGFGYGPGVSAEYDQLVGIISRASGAPVIANAIKYMAEVSRG
                     NLLEVAKDEMKRANKAGLADIIEELKSKSETKEVEEVEMPEKKVTGEEIAGIDILEIE
                     SAQQTLWKEDIYAETGMGCTGPVVLVAEEDEEQAREILKEEGFIH"
BASE COUNT         2776 a         1323 c         1917 g         2506 t
ORIGIN      
        1 agtactaggc tcatctgtaa aagaagatgg ctcaataatt atggaaaata attcaatgtt
       61 ctgtggagac caaattgctg gctggtcccc caaaacaatg aaagaatttt aactccggga
      121 ggtgaattag atgccaaaag caattgtata tttaaatcaa tttttcggac aaattggtgg
      181 agaagaagta gcagaccacg aacctgaaat tagagaggaa aaggttgggg ctgctatgtt
      241 atatgatcaa ttattagacg gtgaagtgag ccacactgta atttgtggag ataacttctt
      301 tggttctaat gaagaagaag ctatagatag aattttagga tttttagaag ataaagaatt
      361 tgatatcttc tttgctggac cagctttcca ggccggtaga tatggaactg cctgtggagc
      421 aatttgtaaa gcagttaaag aagaatttga tgtacctgta atttcttcta tgaacgaaga
      481 gaatccaggt gttgatatgt ttaaaaaaga tatttatatc ttcccaggcg gacaaagtgc
      541 tgccaagatg aaagaagatg taactaagat ggttgaattt ggtaataagg ttcttagtgg
      601 tgaaaagctg cttcctgctg aacaggaagg atactttact agaggtatta gacaccaatt
      661 cttcttacca gatgaaacac ctgcttatga aagagctatt gatatgctgc ttaaaaagat
      721 taataatgaa gaattcgaaa gtgaactacc tataccagaa ttagatactg tagatatagc
      781 agacccaatt gaagacttaa gtgaggcaac tgtagcttta gtaacttctg gtggtattgt
      841 accaattgag aatccagata atattcaatc tgcctcagcc actagatggg gtaagtacta
      901 tattgaagat gttaatagac ttaaaaatag aagaagtgaa gcgtttagaa ccattcacgc
      961 cggttatgac ccggctgcag ctgataccga tcctaaccgt gttgtgcctg tagatgcaat
     1021 gcgtaaatat gaaagagaag gcaagatcgg tgagctttat aagtatttct attcaaccgt
     1081 gggtacagga actacccagg gtgaagctgc tcgtatgggt caagaaatcg ctaaagaatt
     1141 actggaggat ggagttcaag ccgtaatact cacctctacc tgaggtacct gtacacgttg
     1201 cggagcaacg atggtaaaag aaattgaaag agcaggtatc ccagtagtac agatggctaa
     1261 tttaatccct gtagctaaaa cggttggagc taatagaata gttcctacta tttctatccc
     1321 ttatccactg ggagatccat caaccagtga agatgagcag tggaagttac gctaccaccg
     1381 ggtaggagta gctttagatg ctctaactac agacattgat gaacagactg tatttgatgt
     1441 agaaatctaa acaaacatcc tcacctcttc tatgccggga gaggtgagga cattttgatt
     1501 tatgcataat tagtatatgt tcaaaaaaat acttacataa agggtgaatt aaatgaaaac
     1561 gcgtaaagat aaccgaggaa acgtcattaa agaaagagga aaagaagtct ttctaatttc
     1621 tttaattgtt gtttttgcca tagttatctg gggtattgca atgccggaga atttcagtaa
     1681 tgttgccaat agtctatttg attacctaac tggtaacttt ggatggtttt atctgattac
     1741 aatgtctctc tttgtaatct tttgcatttg gatggcgctc agtaaacacg gaaagattag
     1801 gttaggaaaa ccggatgaag aacctgaatt cagcacaatt tcttggtttg caatgttatt
     1861 cagtgctggt atgggtgtcg gtttagtgtt ttggggagtt gcagaacccc taaatcactt
     1921 tgttaatccc cttaatatgc agggcggaac tgcgcaagca gccgattttg ctttaaaaac
     1981 ttcctttttc cactggggat tacatccttg ggcaagttat ggtgtacttg ctttagcttt
     2041 agcatatatg cagtttagac atgataaacc agctcagatc agtagtatct ttattccatt
     2101 aattggtgaa gcaaaagcta gaggaacaat tggtaaagga attgatattt tagctatttt
     2161 tgctaccgtt gctggtgtag caacttcttt aggaatggct acattacagg taacaagtgg
     2221 atttagcttc ctatttggta tccctgaaac caacttagtg agattattag ttattggtgt
     2281 tataacaatc ttatttatga tttcagctat aacaggtgtt gataagggta ttaaatatct
     2341 atcaaatgtt aacatttcat tagctggact tataatggta atttgtttaa ttgttggtcc
     2401 taccgtattg ataattaata actttacaaa ttcatttggt atttatatga gtagtattgt
     2461 aagagatagt tttaagattg gtaaagaacc ctggtacggt tggtggacga tcttctattg
     2521 ggcctggtgg attgcctggg caccatttgt ttccacattt atagcccgta tttccagagg
     2581 tagaactatc agagaattta tcggtggagt attgtttgct cctaccttag cttcttttgt
     2641 atggttttca atcttaggaa ccatgggtat tgaatctgga atgaatgttg ctgaagaagc
     2701 gatcgaggtt actgaaacgg ctttctttgt tgtaatgcag aactttccat tcggtggttt
     2761 gatatccata attgcagtaa tattactaac cacgttcttt attacttcgg ctgattctgc
     2821 tactttcgta cttggcatgt tatcagacaa gggtaatcag aacccgacag ctaaacgcaa
     2881 agtcacctgg ggctttattc agtctggttt agctatagca ctgatgttat cgggtggact
     2941 tggtatgctg cagactggct ctattgtggc agcattccca tttgccatta ttatgatttt
     3001 tgcgatggta gctatgatta aatctgtgaa agcagaacac aaagttgaag gagttgcaga
     3061 tcttaataaa gtggcagata cttctctcga agacttaaag gaagctgatg aactggatga
     3121 agcggtaaat taggattgaa aggagaaaac tcctttacaa aataagatat ggtatttgga
     3181 atcaaaaaat cccggctttt tacagccggg attgattcca gattacttat cacagcagat
     3241 ggcgttgttt acaattgtac ggatatgacc ggtaatttta tttactgcct catcaacgga
     3301 ataatctatt ctattattta ttattaaggt taacatacca tggtagatat aagttatcat
     3361 attgtcaact tcttctgctt tttcttttga gagataatca ttttcgatta gtggttttga
     3421 ggcgatagaa gcacgacgtg aaaggtcagg atccaataac atgggaacta atctttcggg
     3481 tggatctcct aacttttccg gaaaaatttg aaagtatttt tccatcagtg attcaggatg
     3541 gtcacccagt tcttctgtaa agatagcata atagatttgg ggagtttcaa atgaatattt
     3601 acagaaacat tcccacatca ggatgaactt ttcaacttca tcttcgcctt ctgcaatata
     3661 atcaggcatt gcctttatgt agttacttaa aaaatctaat gaagcaaaaa atataagctg
     3721 cttatagtta tcaaagtaat tatagatagt ggcactattg tagccggctc ttttggctac
     3781 atttctaatt gtaacatttt caatgccttt ttcttcaata acttctttgg tagcttgaat
     3841 gaaatattta aggattcgtt ttttcttaat atccttatta ttaatatcga tcattgtaaa
     3901 tcaatccttt cttaattaaa aaagtaatca tgattatacc agttactaca gttaggatat
     3961 aataaaatag agataatttc aagacttgtt gactttagga ctttagtttt atataatttt
     4021 aatgtatgtt tgttgtgaat ataattacta atacagaggg ggaaattcaa atgagtaata
     4081 catatgatct aataattgtc ggaggcggcc cagcaggaat gtcagcagct atttatggtt
     4141 cccgttcccg cctcgatact ttggtgttag aagccaaaag aaagacaggt ggacaaccag
     4201 caacctatca cgatatggaa aactatcccg gtgttccgga aactacagct gatgagctta
     4261 tggataactt cagaaaccat gctgaaaagt tcgatacaga gtttaaaaga ggtgaagtaa
     4321 cagatataac agtcaatggt tttgaaaaag ttgttaaaac caaaaagggt gaagaatata
     4381 aagcaaaaag tgtagtttta gcaatgggag cagaaccgag aaaattaggc atcaaaggtg
     4441 aagaagaact gaaaggtaaa ggagtttctt actgtgcaac ctgtgatgct gacatctttg
     4501 aaggtcttga tgtagtagtt atcggtaatg gtaactctgc agtggaagaa tccatttatt
     4561 tgaccaagtt tgttaacaaa ctgactatgg tagttatcca tgacgaaggc gtaatggatg
     4621 ctgagaagat attacaggaa aaggcaatgg aaaatgataa gatcgacttt gtctggaact
     4681 ctgttgtaga agagatcaag ggtgaagaga tggtcgaagg tgttgtactt aaaaacatca
     4741 aaaccggcga aaaatcagaa ctggactgcc acggagtatt tttctttgta ggccgggttc
     4801 ctaatacaga cttcttagaa ggtactgtag aacttaccga acacggctat ataaaaacca
     4861 atgatgaaat ggaaaccaac caacccggtg tctatggagt aggagatgta agaaataagg
     4921 tagttagaca ggtaataact gcggccggcg atggtgcaac cgcggctgtt atggcccagg
     4981 gctatataga agaagaagaa tactggcaaa ataatgtggt agagtccgat gaacccgtac
     5041 ttgtcggttt ctggagtgta acccaccagg atagtatgga tatgatgagt gagatcgaat
     5101 gcatgaactt agatgaagtg gactgtagat ttgttaagat cgacacttat aaaaacagat
     5161 taatctcaaa tcgttatgac attgatgaaa ttccgactgt tgttaagatt gaagatggtg
     5221 aggttaaagc aaaattagta gaacctaccg tagatgaagt acaagagctt ctataaaact
     5281 ttaaggaggt aaataatatg ttagaactag gaaaagaaaa ttttgaagat gaagtactta
     5341 atgctgaggg agtagtttta gttgatttct ggagtgaaaa ctgtgacctt tgtctagatc
     5401 ttatgccaga tgtggaagaa ttagctgaag aatatggtga ccaaattaaa tttgccaagt
     5461 tagatattaa aggaaataga cgcttagcta tcggtcaaaa agtattgggc cttccttcta
     5521 tcgtattata tgaagacggc gaaaaagttg aacatctcag tggagatgaa ttagaagtag
     5581 aagatatcag agaggcaatt gaagagagac tataaatata aagattaaca tacagctctt
     5641 ctctgtaagg ggaagagctt atttacgaaa aaaattatgg aggtggaaac attgagtttt
     5701 ccagtaataa aaggatcaag ttatatactt gttcacacac caaatatttt aaaacaaggt
     5761 ggttcaactc agaccactac ccgtgctaaa aatcccaatg atgaatattt aaacaagatg
     5821 gatgaacatt tacgtagttt tgaagagttt gtcggttatg cccctaatca agcttacata
     5881 ggaaatatct tgcccaatga tttagaagat atcgagacac cctggtatga tagcgataat
     5941 ctcttagaag ctaaaaaaga gggcgaattc ggcgaaatta tgtccgaaga ggtatttatt
     6001 cctttagtta aacatgtaga tgtttttgat ttagtgaaat taactaaaga attttctaaa
     6061 agagggcagg agttagttaa cgaacatcct atcctaaaag atatggatat tgacttagga
     6121 gaaggagaag aactttcatc cgtagaggaa ttggtggaag cacatgcaga accattttat
     6181 ttagacggta agttagttgg ttgtgtaaaa agtgctcatg aaaccgatat aaatttaagt
     6241 gctcatgtta tttatgagaa cctaattggt aaagcttcag ctgttttagc tatgaaaaat
     6301 cttggctata gaaataatat cgacttatct gaagttgact atattttaga atgttctgaa
     6361 gaggcttgtg gtgacatcaa ccagcgcggt ggcggcaact ttgctaaggc gattggtgaa
     6421 atggccggtg ctactaatgc aaccggttct gatgtaagaa gtttctgtgc agccccggcc
     6481 catgcaatgg ttaatgcagc cgctttagtt aaatcaggtg tatacgataa tgtagctgtt
     6541 gtagcaggcg gctctgtggc taaattgggt atgaatggta aagaccatgt taaaaaagat
     6601 atgcccattt tagaagacgg cctgggtgga tttgccattc taatttctga aaatgatgga
     6661 gaaaaccccg tacttagaac tgatatcgta ggacgtcata gagtgggaac cggttcttcc
     6721 ccacaggcag ttattagttc cttagtaact gatccccttg atgagatgga tattagtatt
     6781 aaagatatag ataaatactc tgttgagatg cagaatcctg aaataacaaa accggccgga
     6841 gcaggtaatg taccagaatc aaattacaag atgattgctg ctctcggtgt taaaagaggc
     6901 gatttggaaa gaaaagaatt aatgaacttt gtagaagaac acggtatgcc cggctttgct
     6961 ccaactcagg gacatattcc ttcaggagtt ccttttgtag gtccagccgt gaaaatgatc
     7021 gaagatggag atattgaaaa tgcaatgatt atcggtaaag gtagtctttt cttaggcaga
     7081 atgaccaatc tctttgatgg tgtatcattt gtaatcgaga ataatgaagg actgcaagaa
     7141 aaagaagcgt ccgttagtga cgaagaaatc aaaagcatga ttgctgatgc catgcgcaag
     7201 atggctgaaa acatgactgt cgagtctaat tgaggtgaat gatatggcag ataaaacaaa
     7261 agaaaaagta gctggaattt ttaatgaact ggcagatgca attgaatccg gacagtttgg
     7321 tgaaaaaaag aaggtgggac ttactttact tgatagtgaa cacggtccag aagaactaca
     7381 gaaagcggct gaaatggcag aaaaaaataa tagtgatata gaggttgaga ccatcggctg
     7441 tgatggtagt aaatgctcat gtttagccga tgctcatgaa gctatggata atatgttaga
     7501 ttcaggtgaa attgatgcct ctgtaacact gcattataac tttccttttg gggtatctac
     7561 agtaggtaga gttattaccc caggccgcgg taaagaaatg ttaattgcta ctaccactgg
     7621 gaccagtgat accgaccgcg tagcttccat gttaaagaac gctgtttatg gaatcgctac
     7681 tgctaaatct ctcggtatta aagaaccaaa acttggtatt ttaaacgtag atggtgcccg
     7741 gttagtagaa aacaacttaa gagaactaaa agataatgga tatgatatcc agtttgctac
     7801 ttctgttaga tctgacggag gagtagtaat gcgcggtaac gacctgctga tgggagtacc
     7861 tgacgttatg gttaccgata ctttaaccgg taatatgtta atgaagatct tttccgccta
     7921 tactacaggt ggaaagtatg aagcttccgg ttttggttac ggtcctggtg taagtgctga
     7981 atacgatcag ttagtaggca ttatctccag agcttcggga gctcctgtga tcgctaatgc
     8041 cattaaatac atggcagaag tgagcagagg taatctactt gaagttgcta aagatgagat
     8101 gaaaagggca aataaagcag gacttgcgga tatcattgaa gaactaaaaa gtaagagtga
     8161 aaccaaagaa gtagaagaag ttgaaatgcc tgaaaagaaa gttactggtg aagaaattgc
     8221 tggtatcgat atcttagaga ttgaaagtgc ccaacagacc ctctggaaag aagatatcta
     8281 tgcagaaacc ggtatgggct gtaccggtcc ggtcgtatta gtggccgaag aagatgaaga
     8341 acaggcccgt gaaatcctaa aggaagaagg ctttatacac taaaaatgat ctattttaac
     8401 aatgcagcga ccacttatcc caaaccagaa tcagtatatc aagacgttga cagctttttt
     8461 agaaatgaag gtgtcaaccc gggaaggtcg cagggtgtaa aaacaatgaa tataaaccgg
     8521 ag
//
LOCUS       DATGCJ010000077         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_277749, whole genome shotgun sequence.
ACCESSION   DATGCJ010000077 DATGCJ010000000
VERSION     DATGCJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277749"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1316)
                     /locus_tag="VKN64_04045"
     CDS             complement(162..1316)
                     /locus_tag="VKN64_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA59442.1"
                     /translation="MEKSPRYDSRIIYLLLSYIVWGIFWGAWGVLLPAVKTAIGATDK
                     SLGFALIGITVGALPAMLGTGLIIKRFSKWVLFYVLLFFAGSIAAITLVNTPLTLAFV
                     LFFVGASSGMLDVVMNSGVATLESLTDKDYFNYAHAAFPVAVIFTSPTVGYVRQSGVN
                     KNIILLVMAGIIALVGLINLKLDMKDNKKEEIKEKGFGYSSVIFQKIILLLGAIGLLV
                     HLLENAVEQWSTIYLEQSLMSSPALASLGITGYMAMLFMGRLIAQRITGKYEDKYILT
                     ISSVISVLGFLLVSMAKIPLLVIIGYSIAGLGIAPIIPLIFSMIGKRTSPEKRVKSIS
                     SVTIIAYTGYLISPPIVGYISNIFSLNTAWIFLTFVSILLVFLIRMIKSA"
     gene            complement(1378..2772)
                     /locus_tag="VKN64_04050"
     CDS             complement(1378..2772)
                     /locus_tag="VKN64_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMA59443.1"
                     /translation="MLIFMTYFPVWKEAFILAWPVILNHIFTTAMRTTDMILMGFFGP
                     AAVTAVGLGDVWERIILRIGLGLGTGSISLISQETGTKTEQARKNADEILSQVIFASI
                     IIGIPFILIGWLIPDTLIRVLGAAPKVVKLGAQYLLIIFSAAPFRIISIIAARAMQGT
                     GDTKTPMVVEIIGNIINIALSIVLALGLGPFPNYGVLGVGIGTFTAKLVSALIYVLIF
                     LSKKSRFNLQMPSKDWDLTIIRQLFKVSMPKILQGIYQSLITFPFNSLVLVFGTEAAA
                     AYHISRRIHQQLIAPLHRSYYTVTTIMGGQKLGAGQPEESKKTTRGMLWLTVFTIGSF
                     AVVLFFTAPWLIRIFTDDTATISFGIRFLKALSIGAPILTIYGVFAGMLNGAGNTKTA
                     LFGNLFSQTTFKLGLSYLLSIIFNLGLIGILIGLVIDFTVRALWVGKKYLKGDWVDEA
                     DKMIEERRIKNKGV"
     gene            complement(3042..3329)
                     /locus_tag="VKN64_04055"
     CDS             complement(3042..3329)
                     /locus_tag="VKN64_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HMA59444.1"
                     /translation="MNKEKTQGETEVLKVASKSSPNSVAGAMAGILREKEYVEAQVIG
                     AGALNQAVKAIAICRGYVAPSGIDLICVPAFLDIVIDGEERTAIKFIIEAR"
BASE COUNT         1339 a          771 c          560 g         1281 t
ORIGIN      
        1 agattggttt tgataaatta actacttttt ccaatatgga acctgagttt cataagatat
       61 aatatgtatt tatcattaaa attattaggt gattacaaag tcctgctaaa attagcagga
      121 cttttgcatt tgtatttata atacatcttt tcaatataaa tttaagctga ttttatcatc
      181 cttataagaa aaaccaacaa tatagataca aaggttaaaa aaatccaggc cgtatttagt
      241 gaaaaaatat tagaaatata cccaacaata ggagggctaa tcaaataccc ggtatatgct
      301 attattgtaa ccgaacttat tgattttact cttttttcag gactggtcct tttaccgatc
      361 atactaaaga ttaatggtat aataggagca attcctaaac cggctattga atagcctata
      421 attactaata atggtatctt tgccatagag actaataaaa atcctaatac agatataacc
      481 gatgaaatag ttaatatgta tttatcttca tacttacctg taattctctg ggcgattaac
      541 cttcccataa aaagcattgc catataaccg gttataccca aactagctaa ggcaggtgaa
      601 gacattaaac tctgttctaa ataaattgta ctccattgtt ctacagcatt ttctaataaa
      661 tggaccaata agccgatagc acctagtaaa agaattatct tttgaaaaat aacagagcta
      721 taaccaaaac ctttttcctt aatctcttcc tttttattat ctttcatatc taattttaaa
      781 ttgattaacc ccactaaagc tataatcccg gccatcacca gtaatataat attcttatta
      841 accccgctct gtcttacata accaacggta ggactagtaa aaataacagc tacaggaaaa
      901 gcagcatgag cgtaattaaa gtaatcctta tcagttaatg attctaaagt cgctactccc
      961 gagttcatta caacatccag cataccagaa gaagcaccca caaaaaataa gacaaaggct
     1021 aaggtcaagg gggtattaac taatgtaata gcagcgattg agcctgcaaa gaaaagcaga
     1081 acataaaaca ggacccattt actgaatctc tttataataa ggccagtacc taacatggca
     1141 ggtaaggccc ccaccgtaat accaattaaa gcaaagccca aacttttatc tgtagctcca
     1201 atagctgttt taacagccgg aagtaatact ccccaggcac cccaaaagat cccccatact
     1261 atataactta ataaaagata tataattcta ctatcatatc tcggagattt ttccatttct
     1321 tttattacct ccaaacaaaa aatgaaaaga ttaaaaataa ttttttgaat atagtattta
     1381 taccccttta ttctttattc ttctctcctc aatcattttg tctgcttcat ctacccagtc
     1441 accttttaga tatttttttc ctacccaaag ggctctgacc gtaaagtcaa taactaatcc
     1501 tatcagtatc ccgattaacc ccaaattaaa gattatactc aataaataac tcaatcccaa
     1561 tttaaaggtg gtttggctga ataagtttcc aaatagagct gtttttgtat taccagcccc
     1621 atttaacatc ccggcaaata ctccatagat cgttaaaatg ggagccccga tacttaaggc
     1681 ttttaaaaat ctaatcccaa aggaaatagt agctgtatca tcagtaaaga tccttattaa
     1741 ccagggcgct gtaaaaaata aaacgactgc aaaactaccg attgtaaaga cagttaacca
     1801 cagcatgccc cttgttgttt tcttgctctc ctctggttgg ccggctccta atttttgtcc
     1861 ccccatgata gttgtcactg tataataaga acggtggagg ggtgcgatta actgctgatg
     1921 gatccttctt gaaatatggt aggcagctgc agcttctgtc ccaaagacta aaaccaggga
     1981 attaaaagga aaggtgatca aactctgata gataccctgc agtatcttgg gcatacttac
     2041 cttaaagagc tgcctgatta tagtaagatc ccagtcctta gaaggcatct gtaaattaaa
     2101 tctacttttc ttacttaaaa atattaaaac atagatcaag gcggaaacga gtttagccgt
     2161 aaaggttcct atcccaaccc ctaatacccc ataattaggg aaaggtccta atcctaaagc
     2221 taatactata gataaggcta tattaataat attacctata atctcaacta ccatcggtgt
     2281 cttggtatct cccgtaccct gcatagcccg ggcggcaatt atagaaataa ttcgaaaggg
     2341 agcagctgag aatattatca aaagatactg agcccctaat tttacaactt taggtgctgc
     2401 tcctaaaact ctaattaatg tatccggaat aagccaaccg attaagataa aaggtatacc
     2461 gataataatg ctggcaaaga taacctgact caagatctca tcggcatttt ttctagcctg
     2521 ttcggtctta gtacctgttt cctgtgaaat tagagaaata ctacctgtcc ccaggcctaa
     2581 tccaattctt aagatgattc tttcccaaac atcacccagg cctactgctg ttacagcagc
     2641 tggtccgaaa aatcccatga ggatcatgtc ggtggtccgc atagcagttg tgaatatatg
     2701 gtttaagata accggccagg ccagaatgaa cgcttccttc cagaccggaa aatatgtcat
     2761 gaagattaac atttgctttc catcctttgt ctaacttgaa aattattata acacaaatat
     2821 tttaaatact acaaaattat ttatagaaaa caaaaaaatt ccggctaatt aaagccagaa
     2881 gcaaattaaa gtataaacac cccaatattt atatgcatta aaaaagaact atttatatat
     2941 ttttagataa aatttgtgct ttctatctta aatatataat atactctttt ttaatgatta
     3001 ctacatacac cttcaaaaag aataacattg tagttgatct gttaacgggc ctctataata
     3061 aatttaatag ccgttctttc ttcaccgtct attactatgt ccaaaaaagc cggtacacag
     3121 atgagatcaa taccacttgg tgccacatac cccctacaaa tcgcaattgc tttgactgct
     3181 tgatttaatg cccctgcgcc aattacctga gcttccacat attctttttc tcttaaaata
     3241 cctgccattg caccagcaac tgagttagga ctagatttag atgcaacttt taaaacttct
     3301 gtttctcctt gcgttttttc cttattcatt tgtaattccc ccttgtgggc cattttaatt
     3361 attaagtttt tatatttgcc atactaatat tgattattct tcttaatgga ttttaatgtt
     3421 attttctctg ttgaaatttt tgaagtaggg aggattattg gaaaatatat aggtatctca
     3481 caaaaatatt ggggttctgt catcttttat ctattaataa atcaattttg gattaatatt
     3541 aatatcacct tcttattttc agtctctata tgttaaattc tttaattaac tcttttctcc
     3601 tttatttcaa tagcattctt gtttatagaa gtaaggcaac aatgtgatta tagaaaattt
     3661 acagataatt tgagtatgtt tatataggtt gaaatgtata taatcacaat atagtttcca
     3721 aaaaatatcg ggatttatac aagttattat taatgcttct ttccaatatt ttatacaaaa
     3781 aacccccaaa ccgaataagg tttagggaat atgattatca ttttaattta tatattatct
     3841 agattagaaa cagttggaaa aagaagtcct taaattattc acctataata agcttaattt
     3901 ctgctcccaa atattttact tcctactctt atcatgttgg ccccttcctt g
//
LOCUS       DATGCJ010000078         8768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_546784, whole genome shotgun sequence.
ACCESSION   DATGCJ010000078 DATGCJ010000000
VERSION     DATGCJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546784"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VKN64_04060"
     CDS             <1..304
                     /locus_tag="VKN64_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59445.1"
                     /translation="IYTYEQMQDPKFVMGFGSCTINGGMYWDSYNTIKQLDKYIPVDL
                     YIAGCMPRPEAILEGFMELKNKIETGNADGYLRYKEDYDFYKENQEKVLGEVKRRQ"
     gene            301..783
                     /locus_tag="VKN64_04065"
     CDS             301..783
                     /locus_tag="VKN64_04065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMA59446.1"
                     /translation="MRNETEVIELIKNKLNPLEVKKLREQNLTITLKNDKLVSGLNYL
                     KSHSYIHLATISCVDWIDEDEFELVYHLFSYQDKINISVKTRIDRKKAKFVTIKHIWA
                     VAQYYERDIHDFYGVEFEGNDDMDELILENWDEIPPMRKDFKTREYVDEKYEWREYHG
                     "
     gene            776..1885
                     /locus_tag="VKN64_04070"
     CDS             776..1885
                     /locus_tag="VKN64_04070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMA59447.1"
                     /translation="MDKSKMTEMFFGPNHPGVPGNYGINYYYEGDTVVSAEPNAGHLH
                     RGFEKLMEEREWIKNTPLVCRICVMEPDINEMVYAMGYEAIGEIEVPKRAQYIRSIIL
                     ELARIQAHIFSIGGNCGAIGLYSAMQIGVLARDYILDIFEEITGGRVYHIYIRPGGVR
                     HDVEEETFAKILNLLDDLENNRMPEFQKLIFENPTAQKRWKDTAILNQEDALRLGVTG
                     PALRATGLQHDVRRLMPYGAYDEVDFDIPTGQDADAWTRLKLKRDEIFQSISIIRQLI
                     NNMPDGPVSVKLGNALKYKIPEGEAYVKVESSRGEYGYYMVSDGGLKPYRVHVRGTST
                     PLGLYGVEALLPGTRIEDVPVWAHSLGICPPEFDR"
     gene            1911..3707
                     /locus_tag="VKN64_04075"
     CDS             1911..3707
                     /locus_tag="VKN64_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA59448.1"
                     /translation="MSEDKKSIFSPTVTFKNLFKKPETVKYPKNSKETAKRYRGFHIN
                     DLDKCIGCGTCAEICDNKAIDMVQLADVKTELGQRDERPKIDYGRCCWCGLCVDVCTT
                     GSLEFTQDYTYIDKDPDTFAYIPDHRNINKVEFEVGYERDDVSELLDLDRVEMEELDP
                     EKRVDSFVEIVQGFSKEQAEKEASRCVECDICTEACPASMNIPEYIRGIFDEDLDESL
                     KQIYEDNPLPEVCGRVCTHKCETICSIGHRGEPISIRWLKRYAVDNVDSAEYQKVLGT
                     EKIDQIDKEVAIIGAGPAGLSAAHYLRLMGYQITVYESEEKAGGMMRYGIPEYRLPYE
                     QLDKDINYIKELGVEFVFNTRVGEDISMQEIEDNYDAVFAATGLHNGRSTRIPGTEHN
                     NVYQAIYLLKQITKGKEVPVTERIVVIGGGNVAMDIARSLARIQKQEFGDVDVLVTSL
                     ETEDIMPADQEEIEEAREEGVRFEPGRGPKEIVVNEDGSISQLCTNCCVSVFDDERNF
                     NPSFDNDDIKNFDCDMVVEAIGQGGDNSYIDDNYDIEYEGPRIKVDEEQQTSLPWLFA
                     GGDIVQGPDVITGIKTGHDAAVGIDKYLRGEK"
     gene            complement(3766..5019)
                     /locus_tag="VKN64_04080"
     CDS             complement(3766..5019)
                     /locus_tag="VKN64_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMA59449.1"
                     /translation="MFLLKLAYKNLFRYWKRTLIAAFIIAMAVFIYLTMDSMLLGMED
                     MTYENIIKYESGHLQIANKEYWEDKDELPLENLISLESRFNENIENINGYIFHSPQLR
                     FQAILNSKGDELPVIGYGINPDQALEVFELKENLVAGTFFTKGERKVVLGSTLAELME
                     VDLDDYITLIFKTSTETFNTIDAQVSGLLETTNPNINNNFVYLPLDLAQDSLSVGDSI
                     SRIVIRLNGKNKVNDAKSQMENLLGNNINKIGVYAWDELEVAEVLSVDQVISKFLLAI
                     ILIIGAMGIINTVILAALERMEEIGMMKALGLKEREIVLTFMYESIGIGIIGVVVGTI
                     LGAITIWYLVNFGMDYAIFMEGGGEEIMGDVGFPMVTEIYSSWNLTAFIIVNTFGLIT
                     SALASIFPAYWAAKKNPVKAIEHRR"
     gene            complement(5058..6311)
                     /locus_tag="VKN64_04085"
     CDS             complement(5058..6311)
                     /locus_tag="VKN64_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMA59450.1"
                     /translation="MFLSKLAFKNIFRHKLRTIISIIVIAFAVMIVIFSRGFINGMIH
                     TLYSDSIQYNSGHVHIMRDEYYKKKRLMPLNYPIDGFEKNKLDEMVNRIENIDDVKGV
                     IYRLKFGAMISTGQDLITLQGWGTNPKKEFELTNIEDKIVEGRMVQSGKLEVIMGTEL
                     LEKIDSRVGDKVTILFNTSYSSLKGVTFNIVGKIESGLKLLDQNVFYLPLEEAQRLLF
                     MEDQVTEMLVMASHRNKSEEIKNKVEDLMKENNVQDKYIVMHYEESNVLLSYLNIAQL
                     VYNQIYFLFVILASVVLISTMIMIVKERTKEIGMMLALGLEEKGIMKLFILEGSIIGL
                     IGSSLGAIIGHYLSSILSEIGFDVGQAVNSVSSDIMFNKIIYPTSSVGNSLFAFFAGI
                     VIVILVSVIPARRAAKLEPTEAMKE"
     gene            6525..6986
                     /locus_tag="VKN64_04090"
     CDS             6525..6986
                     /locus_tag="VKN64_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HMA59451.1"
                     /translation="MKTKEKQNIVEEVRQTAEKYFRDGDYYCSESVLTTINDALGQEF
                     RPEIVKLASGFPIGVGKSGCLCGAISGGVMALGLAYGREKPGASMPKSFPNNAALHDF
                     IIEKYGSTCCRALTKDFEDFASKERAEHCIKITGEVAAWVMKNFIENGVIK"
     gene            6997..7914
                     /locus_tag="VKN64_04095"
     CDS             6997..7914
                     /locus_tag="VKN64_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HMA59452.1"
                     /translation="MTKIFKSVPIPIAGLMLGMAGLGNLISTYSIQYRYLAGFISLII
                     GILLLGRLLIDYENVFKELKHPILGSVALCFPMGIMILSTYFLNIFKPAASFLWYSGL
                     ILHIFLLILFSIRLINNINIKKVFPSFFIVYVGIVVASVTAPAFNHIKLGQYIFYFGF
                     ISYLMLLPIILYRVVKIGKIKRPALPTIAIFTAPAGLCLTGYMSVFPQKNIMFLGCLI
                     TLTLLSVIFVIIYLPKLLSKEFYPSFSAFTFPFVITAIGLKLSNNFLLQKYNISYLKY
                     SVILLEIISIILVIFVLFKYTKFLFEKCT"
     gene            complement(7958..8401)
                     /locus_tag="VKN64_04100"
     CDS             complement(7958..8401)
                     /locus_tag="VKN64_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59453.1"
                     /translation="MKENEGLKNNLVMILRLIARIWAIIVLIIAIIFLVGYLYNWVTL
                     GTADPHQVEEAHPPLVFLGIIGLAIALKREKSGSIITILAGILHLGEEIYLWIFNSEV
                     PRLITYIFVDELNYSAQYYLTPYIVVLFIITPGILFLITGFYSNR"
     gene            complement(8532..>8768)
                     /locus_tag="VKN64_04105"
     CDS             complement(8532..>8768)
                     /locus_tag="VKN64_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA59454.1"
                     /translation="IIKDIKKRTNQKIAGILGGLHLKHSSGKEVIEIMNYFDSINFDL
                     LVPIHCTGREAAMLFKDHFEKRVKLSSVGDNFTF"
BASE COUNT         2984 a         1410 c         1484 g         2890 t
ORIGIN      
        1 tatctacact tatgaacaga tgcaggatcc caagtttgtg atgggttttg gttcctgtac
       61 gataaatggg ggtatgtact gggattcata taatacgata aaacagcttg ataagtatat
      121 accggttgat ttatatatag caggctgtat gccccggccg gaagcgatcc tggaaggttt
      181 tatggaactt aaaaacaaga tcgaaaccgg gaacgcggat ggatatttaa gatacaaaga
      241 agattatgat ttctataaag aaaatcagga gaaagttctg ggggaggtta agagaagaca
      301 atgagaaatg aaacagaagt aattgaactt ataaaaaata aattaaatcc tctcgaagta
      361 aaaaagttaa gagaacaaaa tctaactatt actttaaaaa atgataaact ggtttctggt
      421 ctaaattact taaaaagcca cagctatatt catcttgcta ctatcagctg tgtagactgg
      481 atagatgagg atgaatttga actggtctat catctcttct cttatcagga taagataaat
      541 attagtgtta aaaccagaat tgaccgaaaa aaagctaagt ttgtaactat aaaacatatc
      601 tgggcagttg ctcagtacta tgaaagagat attcatgatt tttacggtgt tgaatttgaa
      661 ggaaatgatg atatggatga acttatctta gaaaactggg atgaaatacc tcccatgcga
      721 aaagacttca aaacaagaga atatgtagat gaaaaatatg aatggaggga ataccatgga
      781 taaaagtaaa atgacggaaa tgttttttgg gcctaaccat ccaggtgttc cgggtaatta
      841 tggtataaat tactattatg aaggagatac tgttgtctca gctgaaccta acgccggcca
      901 cttgcaccgc ggatttgaaa agcttatgga agaaagagaa tggattaaaa atactccttt
      961 agtctgccgg atctgtgtta tggagccgga catcaatgaa atggtctatg ccatgggata
     1021 tgaagctata ggagaaattg aagtaccaaa aagagctcaa tacattaggt caatcatcct
     1081 agaactggct agaatccagg cccatatctt ctctattgga ggaaactgtg gggcaattgg
     1141 gctttattca gctatgcaga tcggagtttt ggcccgtgat tatatcctcg atatattcga
     1201 agagattacg ggtggtagag tgtatcatat ctacatcaga ccaggtgggg tcagacatga
     1261 tgtcgaagaa gaaacctttg caaaaatatt gaatctatta gatgacttag aaaataatag
     1321 gatgcccgaa tttcaaaagt taatctttga aaatcccact gcccaaaaac gctggaaaga
     1381 tacagctatt ttaaatcagg aagatgccct aagactaggt gtcacaggtc cagctttaag
     1441 ggcaaccggt cttcaacatg atgtcagaag attgatgcct tatggggctt atgatgaagt
     1501 agattttgac atcccaaccg gccaggatgc tgatgcctgg accagattaa aattaaaaag
     1561 agatgaaatc tttcaaagta taagtattat tagacagtta atcaataata tgccggatgg
     1621 accagttagt gttaaattgg gtaatgcctt aaagtataag atacctgaag gtgaagccta
     1681 tgtaaaggtt gaatcttcac gcggagaata tggttactat atggtctctg acgggggatt
     1741 aaagccctat agagtccatg taaggggtac atcaacccct ctcggtttat acggtgtaga
     1801 agcactgctg ccgggtacta gaattgaaga tgtaccggtc tgggcccata gtttgggaat
     1861 ctgcccaccg gaattcgata gataatattt ctcatagtga ggtgaatttg atgagtgaag
     1921 ataaaaaaag catattttct cccacagtta catttaaaaa tttatttaaa aaaccggaaa
     1981 cggtcaaata ccctaagaat agtaaagaaa ctgcgaagcg ttatcggggc tttcatataa
     2041 atgacttaga taaatgtatc ggttgtggta cctgtgcaga aatttgtgat aataaagcaa
     2101 ttgacatggt tcaattagct gatgttaaga ctgaattagg acaaagagat gaaagaccaa
     2161 agattgatta cggtcgttgt tgttggtgtg gtttgtgtgt ggatgtttgt acgaccggtt
     2221 ctctggaatt tacccaggac tatacttata ttgataaaga tcctgatacg tttgcctata
     2281 taccagatca tagaaatatc aacaaagttg aatttgaagt tggttatgaa agggatgatg
     2341 tatctgagct gctcgattta gaccgggttg aaatggaaga attagatccg gaaaaaagag
     2401 tcgactcttt tgtagaaata gttcagggtt tttccaaaga acaggctgaa aaagaagcat
     2461 cccgttgtgt ggaatgtgat atctgtacag aagcctgtcc ggccagtatg aatataccgg
     2521 aatatatcag ggggatcttt gatgaagatt tagatgaatc attgaaacaa atttatgaag
     2581 ataacccctt accagaagta tgtggacggg tctgtacaca taaatgtgaa actatctgtt
     2641 caatcggaca ccggggagaa ccgatctcta tccgctggct gaaaagatat gcggttgata
     2701 atgttgactc agctgaatat caaaaggtac tgggtaccga aaaaattgat cagattgata
     2761 aagaagtagc tatcatagga gctggaccag ctggtctttc tgccgcccat tatttaaggt
     2821 taatgggtta tcaaataaca gtatatgaat cagaagaaaa agcgggtggc atgatgagat
     2881 atggtatccc ggaatataga ttaccctatg aacagcttga taaagatatt aactatataa
     2941 aagaactggg agtagaattt gtctttaaca ctagagttgg cgaagacatt tccatgcagg
     3001 aaattgaaga taattatgat gcagtctttg ctgctaccgg tctacacaat ggcaggagta
     3061 ctagaatacc aggaaccgaa cataataatg tatatcaggc tatttatctt ttaaagcaga
     3121 ttactaaagg taaagaagtc cccgttacgg aaagaatcgt tgtaattggc ggcggtaatg
     3181 tggccatgga tatcgctcgt tccttagcta gaatacaaaa acaagaattc ggggatgttg
     3241 atgtcttagt tacctcttta gaaactgaag atatcatgcc ggctgatcaa gaagaaattg
     3301 aagaagcccg cgaagaaggc gtcagatttg aaccgggccg gggcccaaaa gaaatcgttg
     3361 taaatgaaga tggaagcatt tcacagcttt gtacaaactg ctgtgtatct gtctttgatg
     3421 atgagaggaa cttcaatcca tcctttgata atgatgatat taaaaacttt gactgtgata
     3481 tggtagtaga ggctatcggt cagggtggtg acaatagtta tattgatgat aactatgata
     3541 ttgaatatga aggacctcga ataaaagttg atgaagagca gcagacctct cttccctggt
     3601 tatttgcagg cggagatatt gtccagggac ctgatgtaat tactggtatt aaaacaggtc
     3661 atgatgcggc agtaggtatt gataaatact tacggggaga aaaataataa ataagtcatt
     3721 gggggagtgt tttcaaactc tccccttttt atttgtaata tatttttacc ttcgatgttc
     3781 aattgctttt accggatttt tttttgcagc ccaataagcc ggaaaaatgc ttgctaaagc
     3841 agaggttatt aaaccaaatg tattgactat aataaatgca gttaggttcc aagaactata
     3901 aatttcagtc accatgggaa aacctacatc acccattatt tcttcaccac cgccctccat
     3961 aaaaatagca taatccatac caaaattaac taaataccag atagttatag ctcccaatat
     4021 tgttccgact actaccccta tgataccaat gccgattgat tcatacataa aagttagtac
     4081 tatttctctt tcctttaaac ctaatgcttt catcattcca atttcttcca ttctttcaag
     4141 tgcggctaat attactgtat ttattatacc catagctcca ataattaata taattgctaa
     4201 aagaaattta gaaatcacct gatccacact tagaacttcc gctacttcta attcatccca
     4261 ggcataaact cctatcttat ttatgttatt acccaataaa ttttccattt gactttttgc
     4321 atcatttact ttgtttttac cattcaatcg tatgactatt ctactaatac tatctcctac
     4381 agacaatgaa tcctgggcaa gatctaatgg taaataaaca aaattattat taatattagg
     4441 attagttgtt tctaaaagac ctgatacctg ggcatctatt gtattaaaag tttcagtaga
     4501 agttttaaat ataagagtta tataatcatc taagtcaact tccattaatt ctgctaaagt
     4561 acttcctaat acaacttttc tttctccttt tgtaaaaaat gtacctgcta ctaagttctc
     4621 tttcaattca aatacttcta aggcctggtc tggattaatt ccataaccaa taaccgggag
     4681 ttcatctccc ttgctattta atattgcttg aaatcttagt tgaggagaat gaaagatata
     4741 tccatttatg ttttcaatat tttcattaaa tctagactct aaagatatta aattttctag
     4801 cggtaactca tctttatctt cccaatattc tttattagct atctgaagat gacctgattc
     4861 gtattttatt atattttcat aagtcatatc ttccatacct aataacatag aatccattgt
     4921 caggtatata aatacagcca tagctataat aaatgcagca attaaagtcc ttttccaata
     4981 acgaaataga tttttataag ctagctttaa taaaaacaaa gaaagacacc ccctacctaa
     5041 ttattaatat atttttatta ttctttcata gcttctgttg gttcaagttt cgccgccctc
     5101 ctggcaggta ttacactaac taatatgact ataacaatac ctgcaaaaaa agcaaataaa
     5161 ctgtttccaa ctgaagaagt tgggtatata attttattaa acattatatc agaactgaca
     5221 ctattcactg cttgcccaac atcaaaacca atttctgata aaatactact cagataatgc
     5281 ccaattatag cacctaaaga acttccaatt aaacctatta tactgccttc caaaataaat
     5341 aatttcatga tacctttctc ttccaaaccg agagcaagca tcatgcctat ttctttcgtc
     5401 ctttctttta caatcataat cattgtactt attaagacta cactagcaag aataacaaat
     5461 aaaaaataaa tctgattata cacgagttga gctatattta aataagaaag taatacattt
     5521 gattcttcat aatgcattac tatgtattta tcttgaacat tattttcttt catcaaatct
     5581 tctactttat ttttaatttc ttcagactta ttacgatggg aagccattac taacatctca
     5641 gtaacctgat cctccataaa taataacctc tgtgcttctt ctaaaggaag gtaaaaaaca
     5701 ttttgatcta aaagtttaag gccactttca attttgccca caatattaaa tgtaacaccc
     5761 tttagagaac tataggaagt attaaataaa atagttactt tatctcccac tctggaatct
     5821 attttctcaa gaagttcagt tcccataata acttctaact ttccggactg taccatccga
     5881 ccttcaacaa ttttatcttc aatattagtt agttcaaact cttttttagg atttgtaccc
     5941 catccctgca atgttattaa atcttgcccc gtactgatca tagctccgaa tttcaatcga
     6001 taaattacac cctttacatc atctatattt tctatcctat ttaccatttc atctaattta
     6061 tttttctcaa acccatcaat tggataattt agcggcatca gccttttctt cttatagtat
     6121 tcatctctca taatgtggac atggccggaa ttatattgaa tagaatctga atacaaggta
     6181 tgtatcatac catttataaa gccacgggaa aaaatcacaa tcattaccgc aaaagcaatg
     6241 acaataatag aaatgatcgt tcttaattta tgtctgaata tatttttaaa agccagtttt
     6301 gataaaaaca tcataaattc tcctctcaat taaataagag tgactgtttg ttcatatgac
     6361 tttatattca tattatactc ttatatatac aaaaaatcaa aacaaatagt ttttgaaaat
     6421 tatttataat aatatcttat taaatttata agattacttg atatttaatc atagttatga
     6481 tattttattt atagttgtga aatattttac taggagtggt taatatgaaa accaaagaaa
     6541 aacaaaatat tgtagaagaa gtaagacaaa cagctgaaaa atattttaga gatggtgatt
     6601 attactgttc ggaatctgtg ctaacaacta ttaatgatgc ccttgggcaa gaattcagac
     6661 cagaaatagt gaaattagcc tctggatttc caattggggt aggaaaatct ggctgtttat
     6721 gtggtgcgat aagtgggggt gtgatggctt taggtttagc atatggaaga gaaaaaccgg
     6781 gagcaagtat gcccaaatcc tttcccaata atgctgcctt acatgacttc attattgaaa
     6841 aatatggatc tacttgttgt agagcactta ctaaagattt tgaagatttt gccagcaaag
     6901 aaagagctga acattgtatt aaaattacag gtgaagtagc agcctgggtt atgaaaaact
     6961 ttattgagaa tggcgttata aagtaggtga aataaaatga caaaaatttt taaaagtgta
     7021 ccaataccaa tagcaggttt aatgttagga atggcaggat tgggtaattt aattagtact
     7081 tatagtattc aatatagata tttagctgga tttatttcat taataatagg aattttatta
     7141 ttaggaagat tattaattga ttatgaaaat gtatttaagg aacttaaaca ccctatttta
     7201 ggtagtgtag ctctctgttt tccaatgggg attatgatat tatctacata ttttttaaat
     7261 atttttaagc cggctgcttc ttttttatgg tattcaggct taatcttaca tatattttta
     7321 ttgatactat tttctataag gttaataaat aatataaata ttaaaaaagt attccctagt
     7381 ttttttatag tatatgttgg gatagtggtt gcaagtgtaa ctgctcccgc ctttaatcat
     7441 attaaacttg gtcaatatat attttacttt ggttttatct catatttaat gttactacct
     7501 ataatattat atagagtagt taaaattgga aaaataaaaa gaccagcttt gcctacaatt
     7561 gctattttta ctgctccagc tggactttgt ttgacaggtt atatgagtgt tttccctcaa
     7621 aaaaatataa tgtttttagg atgtttaatt actttgacat tattatcagt aatatttgtg
     7681 ataatttatt taccaaaatt gttatcaaag gaattttatc ctagtttttc agcttttact
     7741 tttccttttg ttataacagc tattgggtta aaattatcaa ataattttct tttacaaaaa
     7801 tataatataa gctatttaaa atattcagtg atattactag agataatttc tataatttta
     7861 gttatctttg ttttattcaa gtatactaag tttttatttg agaaatgcac ctaatatatt
     7921 aataactgaa tttctgattt tattttaatt ccacttttta tctatttgaa taaaaaccag
     7981 ttattagaaa taaaatacca ggtgttatta taaataatac aactatatag ggagttagat
     8041 aatattgagc agaataattt aattcatcaa caaatatata agtaattaat ctgggaactt
     8101 ccgaattaaa aatccataga taaatttctt cccctaaatg taaaattccc gctaaaatcg
     8161 taataatact gccacttttc tctcgtttta aggcaatagc taatcctatt atacccaaaa
     8221 ataccagagg tggatgagct tcttctactt gatgaggatc tgcagttcca agggtaaccc
     8281 aattatataa atatcccact aaaaaaatta tagcgattat tagtacgatt atagcccata
     8341 tcctggctat caatcttaat atcataacca gattattttt taaaccttca ttttctttca
     8401 ttttattttt ccttctttat taaaagttct aataataaaa aagtctctct tttttctact
     8461 tctattatat caaaaagaga gactttctcc tatccttttt attcaataaa gaaaatgatt
     8521 tttatttgtt tttaaaaagt aaaattatca cctactgaag ataatttaac tcttttttca
     8581 aaatggtcct taaacaacat ggctgcctct cgtcccgtac agtgaatagg aactaaaaga
     8641 tcaaaattaa ttgaatcgaa ataattcatg atttcaataa cttcttttcc agaggagtgc
     8701 tttaaatgca gcccccccaa aattcccgct atcttttgat tggttctctt tttaatgtcc
     8761 tttattat
//
LOCUS       DATGCJ010000079        16256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_277977, whole genome shotgun sequence.
ACCESSION   DATGCJ010000079 DATGCJ010000000
VERSION     DATGCJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277977"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1229)
                     /locus_tag="VKN64_04110"
     CDS             complement(159..1229)
                     /locus_tag="VKN64_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HMA59455.1"
                     /translation="MINPILEKYADVLVDYSVDVEKGDQVLIKGAMVAEPLIKALYIK
                     ILKKGAHPLVVPTFEGQEELFFEYASKHQLDYQSPYQKYVVENVDASISILAHYNTKG
                     MTNVDPEKMKTRSLANRELMEIHMKRAAEGDLNWTICQYPTSADAQEAKMSLHEYEDF
                     VYKACHLYEDDPVAYWQELGKELEGYADYLNDVKELHYKSKDTDIKFNVEGRIWIPDK
                     GQENYPGGEVFTGPVEDSAEGHIRFSYPGIFQGKEIEDIRLTFENGKVVEASAEKGEE
                     LLHTLLDTDEGSRYLGEVAIGCNHGIKQFSRNMLFDEKIGGTVHLALGRSYPETKGEN
                     ESTIHWDMLCDMTDGGQIFADG"
     gene            complement(1246..1617)
                     /locus_tag="VKN64_04115"
     CDS             complement(1246..1617)
                     /locus_tag="VKN64_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59456.1"
                     /translation="MSEERLQILKMLEKGTIDAEEANELLGTLEKRDTVFEKPETSGK
                     KPKFIKILVEENGQEKVNISIPMLLAKTALKIMPKKAKQQMEEQNVNLDEILSAINDE
                     SSKGKIVDINDGGDHVSISLE"
     gene            complement(1634..2011)
                     /locus_tag="VKN64_04120"
     CDS             complement(1634..2011)
                     /locus_tag="VKN64_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HMA59457.1"
                     /translation="MIHNLVGNCPVCGDDMIITELQCPSCETTISGKFKLDKFSRLKK
                     EHLEFIEVFVKSRGNIKEVERELGISYPTVRNKLDEVIHALGHDIEQSPDDEMAEKRK
                     EVLTRLESGEISAAEAVEQLGEI"
     gene            complement(2141..3313)
                     /locus_tag="VKN64_04125"
     CDS             complement(2141..3313)
                     /locus_tag="VKN64_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HMA59458.1"
                     /translation="MNRYFADLHIHIGASKNGEPVKITASNSLTFENIVRESSERKGL
                     DLIGIIDCASPRVQADIEDMLDKGEIKEIDGGGFNFQDEIVIIPGAEVESREANGGMV
                     HYLAYFPFLDNLKEFTTIMKQYITNVNLSSQVTGLTGSQILKIIDATGGIMVIAHAFT
                     PHKSFYGNAVESYQKVFTKKEWDKIPAIELGLSADTDLADRLSELKEKTFLSNSDSHS
                     IPKIAREYNIFKMQELNFKEFKKTLLRKDKRRVVSNFGLDPRLGKYHRSFCPACQQKF
                     TDEKPYSYCPNCGKELIKGVKDRILEISDQEIKHPEYRGKYIYQIPLLDIPGIGKKTL
                     QKLLQEFDTEMDVIHDTTISELKKILNDQLAEKIDLARRGEAKIVSGGGGNYGKMK"
     gene            complement(3306..3851)
                     /locus_tag="VKN64_04130"
     CDS             complement(3306..3851)
                     /locus_tag="VKN64_04130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMA59459.1"
                     /translation="MDKYKEEILESEYIYEGSILNLRKDKVKLYNDHETYREVVEHSG
                     GVAIVPVTEEGKILMVKQYRIAVDEVVLELPAGKLEEDEDVKKCAARELEEETGFRPA
                     DIKELFHFYTTPGYSSEKLHLFIADGLKHYGQNLDLGEYIEIVELEEEEIIPNILNGK
                     IKDSKTIIGLLWYLNKEAKDE"
     gene            complement(3966..4499)
                     /locus_tag="VKN64_04135"
     CDS             complement(3966..4499)
                     /locus_tag="VKN64_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA59460.1"
                     /translation="MNEEIIMAGFGGQGIMLMGKLLSYAGMKEDKEVSWMPSYGPEMR
                     GGTANCTVIVSDKKIPSPMSSKPDTIIVMNLPSLDKFLSQVKSGGTVFMNSSLIEKEV
                     ERDDVKVIKVPANKIANELGNSRVANMVMLGSYLEEKDIVKPATVKKSLEDVLSARNQ
                     DLIDMNVKALDRGKEYK"
     gene            complement(4501..5247)
                     /locus_tag="VKN64_04140"
     CDS             complement(4501..5247)
                     /locus_tag="VKN64_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA59461.1"
                     /translation="MKQVAGYPDALTDKPFHYCPGCTHGIIHRIVAEVIDELDLREDT
                     IGVAPVGCSVLAYEYFNVDMHEAAHGRAPAVATGIKRVHPDKFVFTYQGDGDLASIGT
                     AEAVHAANRGELISTIFVNNAIYGMTGGQMAPTSLEGQKTTTSPYGRDLKEAGHPIKM
                     SEMLTELDGSAYIARVSVHDPKHIRQAKKAIKKAFQMQLDGEGFALVEVLSTCPTNWG
                     MKPQDAIEWLKNNMIETYPLGELKSPEGVE"
     gene            complement(5247..6308)
                     /locus_tag="VKN64_04145"
     CDS             complement(5247..6308)
                     /locus_tag="VKN64_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HMA59462.1"
                     /translation="MGEKTLMKGNEAIAEAAIRAGCRYFFGYPITPQSELPAYLARKF
                     PEVGGTFLQAESEVAASNMIYGAAGAGARVLTSSSSPGISLKAEGISYIAGAELPAVI
                     INIMRGGPGLGSIQPSQADYFQATKGMAHGDFSLAVLAPSSVQEAVDLTMLAFDLADK
                     YRNPVLLVGDGIIGQMMEPVEFKDPVDPEDLPKKEWATDGAKGREPNVINSLALDPQD
                     LEDHNMKLKAKYDKMKEEEVRYEELYTDDADTIIVAYGTTARIASRAIEMARKDGHKV
                     GMIRPITLFPFPTDIINETADRVDNFLAVEMSTGQMVEDVRLAVNGKKPVHFYGRTGG
                     VVPSPEEIVEEIEKIGGEK"
     gene            complement(6333..6557)
                     /locus_tag="VKN64_04150"
     CDS             complement(6333..6557)
                     /locus_tag="VKN64_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA59463.1"
                     /translation="MEKEKHKNVEFDIERCKGCELCVSVCPVSIISMADDINSHGYHY
                     AQVKEQDKCISCGMCATMCPDVVISVYKEE"
     gene            complement(6570..7637)
                     /gene="buk"
                     /locus_tag="VKN64_04155"
     CDS             complement(6570..7637)
                     /gene="buk"
                     /locus_tag="VKN64_04155"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HMA59464.1"
                     /translation="MEMLILTINPGSTTTKLGLYNRTTEIKKLNIDHDIKDLKNFARI
                     SDQLEFRYNYILEFLQKEDIEIKNIAAVVGRGGLLRPIDGGTYRVNEKMKEDLKEAKR
                     GEHASNLGGLLADLLAKKADCPAFIVDPVVVDEMQEIAKISGIPELRRKSILHALNQK
                     AVARKYSEIVNKEYKDINLIVAHLGGGISVGAHKKGKIIDVNNALSGDGPFSPNRSGG
                     VPSYDLFEMCFSGEYEKEEIKKKLIGKGGVAAYLDTSNMLEVENRIDQGDEKAQLIFE
                     AMAYQVAKEIGSMAAVLSGNCEVIILTGGIAYSDRFVHLIENRVRHFSPVKVFAGEEE
                     LEALAAGAYRVLSGIEKAKNY"
     gene            complement(7648..8712)
                     /locus_tag="VKN64_04160"
     CDS             complement(7648..8712)
                     /locus_tag="VKN64_04160"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMA59465.1"
                     /translation="MEELFKRLELDDYEQVVFNYDEASGLKAIIAIHDTTLGPALGGT
                     RIWDYDTEEEALNDVLRLAKGMTYKSAAAGLNLGGGKAVIIGDPKEIKSEELWRAYGR
                     FVQSLNGRYITAEDVNTTVDDMHIVAEETDYVTGLPSTSGDPSPVTALGVFHGIRASA
                     DEVYGTDDLNGKVVAVQGVGHVGYYLVKHLKEAGADVVVTDIKQENIDQVVEDFGVRV
                     VEPGKIYSVDCDIFAPCALGAVINDDTIPQLNCDIVAGAANNVLEEERHGDVLEEKGI
                     VYAPDYVINAGGVINVYDELIGYDRERALKRAKNIYNNIKKVYEISKRDDIPTYKAAD
                     IVAEERINNIGKVRRNFIPR"
     gene            complement(8728..9639)
                     /locus_tag="VKN64_04165"
     CDS             complement(8728..9639)
                     /locus_tag="VKN64_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HMA59466.1"
                     /translation="MLESIEELIDEATKTKPLKLAVVAAADEVVIDSIEKATNLGIVE
                     PILIGDYAKIDEIAQAKGLKDKVEIVGTTSQKKSAHKAMQLIKEGRADYPMKGHISTS
                     IILKALLDKQYGLRGENILSLVTLIYLEKEERFVIMTDGGMNISPSLEDKIDLINNAG
                     RMAHAIGVENPICAVLAAVEAVNPSMEATLEAASLSKMSDRGQIEGITVDGPLAFDNA
                     ISKEAAEHKGIDSPVAGKADILLVPDIEAGNILYKSLVFYGEQPSASVVVGAKVPLVI
                     TSRADRAETKLNSIALGKVMTEDKNNI"
     gene            complement(9644..10711)
                     /locus_tag="VKN64_04170"
     CDS             complement(9644..10711)
                     /locus_tag="VKN64_04170"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMA59467.1"
                     /translation="MELFKEMEKHGHEQVMFLQDNKVNLKAIIAIHNTVLGPSLGGCR
                     MWDYDKEEDMIIDALRLSKGMTYKSGISGVDFGGGKTVIWGDPNKDKSEGIFRALGKF
                     INTLNGRYSTGTDVGTTYDDFVILRKETPYVGALPKEFGGSGDSSIATAYGVFHGIKA
                     TCKYKYGKDDCDGLTVAVQGLGKVGSKLTKHLIDAGCEVFATDINEDYIEEGRELGAK
                     IVEPDEIYDVDCDIFSPNALGSVINEDTIDRLKCDIVAGAANNVLENHDFAHKLQERD
                     ILYAPDYVINAGGLIQVADELGIGGYSEERTMKKCEGIYNKLLQIFEVADEKGITTVE
                     AADHMVEERIEMMKAVHNIRG"
     gene            complement(10940..12700)
                     /locus_tag="VKN64_04175"
     CDS             complement(10940..12700)
                     /locus_tag="VKN64_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HMA59468.1"
                     /translation="MSKKNKEARIKELKNIIRKKNVIINSTHDGLISVDENGRIELFN
                     DAAENMFALDRKEVLNKKVEEVIPNTRLHHVLVNGEAELNQIQHTHETTIITNRVPVK
                     DKEENIIGAVAVFRDITEVKNLAEEITNLREIRSMLEAIINSTQDAISVVDEDGVGIL
                     INPAYTKLTGLTEEDIIGKPANVDIAEGRSMHYKVLETEKPVKGVRMKVGPNKKDVIV
                     DAAPIIVDGELKGSVAVAHDVSEIKKLTEELDQAKQIIRQINAKYSFQDIVGESDTMQ
                     AVINQAEKASKTPITVLLRGESGTGKELFAHAIHNNSERSQQKFVRVNCSALADNILE
                     SELFGYTEGAFTGAKKGGKKGLFEEADQGTIFLDEIGKISMNLQSKLLRVIQEKEILR
                     VGGTRPIDIDVRIIVATNINLEKAIQDGKFREDLYYRLNVFPIFIPPLRERKKDIPFL
                     IHHIITKFNQEYGRSVKGCSKEAINYLKKYEWPGNVRELENIIGRAMINLGLNEDIIE
                     IEHIPDITGASEEAEDLVKSGAQYSLDSNSLKDIVNRTEKQVIQKALKETGGNRKEAA
                     EILGIAVRSLYYKINKYEID"
     gene            complement(12721..13389)
                     /locus_tag="VKN64_04180"
     CDS             complement(12721..13389)
                     /locus_tag="VKN64_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59469.1"
                     /translation="MIDKRVTIFTGNFGSGKTEISLNYALHLREKNNKVVVIDLDTIN
                     PYFRSREKNELMEAEDIEVIYPKELKYADLPIITPDIKKLLQNDEYHGVIDVGGEEDG
                     ATVLGSIADDVRGIDYELNLVINTNRPFTEDVEGIIKMKEKIESASKLDVDNLVCNIN
                     LGEETTIEDVKNGYPLVKEAAQELNLSIKFISVREDLKGQLEKLDYEEEIFTIKRFLK
                     NPWD"
     gene            complement(13395..14393)
                     /gene="argF"
                     /locus_tag="VKN64_04185"
     CDS             complement(13395..14393)
                     /gene="argF"
                     /locus_tag="VKN64_04185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMA59470.1"
                     /translation="MAFNLKGRSLLSLQDFTKEEISYLLTLAKNLKDQKRTGLTGKTL
                     EGKNIALIFEKPSTRTRCSFSVAAKDEGGMPEYLGKNDIQLGKKETVADTARVLGRYY
                     DGIEFRGFKHETVEQLAEYAGVPVWNGLTDQFHPTQILADFLTIEENFDRLEGINLVY
                     VGDGRNNMANSLMMGSAIMGLNYRIVAPKELWPKQELVDKAKELAKNSKATIEITDDP
                     VKGVKDADAIYTDVWVSMGEEEKFAERIEMLKPYQVNMEMIENTGNEDVIFLHCLPAY
                     HNTDTENGQKIYEEYGIKEMEVTDEVFESKYSKAFDEAENRMHTIKAVMVATLGNQ"
     gene            complement(14495..15295)
                     /gene="spo0A"
                     /locus_tag="VKN64_04190"
     CDS             complement(14495..15295)
                     /gene="spo0A"
                     /locus_tag="VKN64_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HMA59471.1"
                     /translation="METDVKEVVIIDQDEEFCQKFETELNQTEDFACAATATDGHEGL
                     QLIFREDPDYVLMDLILPYLDGVSVLDELREEEKIEDVNIIVLSSFLNDQMLKILQNY
                     EIDYFMSKPADFDCIKNRITDLDQDYTLSNYESSLAEQLNSDENEVNMNLEITGLLDE
                     FGVPANIRGYMYLRNAIYLSITDLELVNSITKQLYPKIAENFSTVASRVERAIRHAIK
                     VAWERGNRDKISEYFGYSISETIGRPTNAQFIAKVADDFRLRNRLITN"
     gene            complement(15477..>16256)
                     /locus_tag="VKN64_04195"
     CDS             complement(15477..>16256)
                     /locus_tag="VKN64_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMA59472.1"
                     /translation="EERLDLINRLKRKYGDSIEEILEYKDDLKEKLSELKNQEKIIDE
                     LENKLSKLKNQYDKKAKELSDIRKEAAEKFEEDLKNELKDLAMEKARLKVKFAKAKRT
                     AEGIDDVKFLISTNPGEDLKPLSRIISGGELSRIMLAFKNIMADIDRVETLVFDEVDK
                     GIGGKTAQKLAEKLYRISNKRQVVCVSHLPQVASMGDNHYYINKETNEEKKTVTNIKK
                     LNEKETIEELARMLGGVKLTDTTKRHAREMLKMAEEKQQSI"
BASE COUNT         4443 a         3606 c         2440 g         5767 t
ORIGIN      
        1 agcccagcaa agaatcgttg aagcagctgc agaatactta taaattatga agataactaa
       61 atatcactaa aaaaccggta ggttttatcc taccggttat ttttagatta tttttttata
      121 taaaattaga tcagaaattc accatcttca tagattagtt aaccatctgc aaagatctga
      181 ccaccatcgg tcatatcaca gagcatatcc cagtgaattg tagattcatt ttcacctttg
      241 gtttcaggat aacttctccc taaggcaagg tgaactgtgc caccgatctt ttcatcaaaa
      301 agcatgttcc ggctgaactg ttttataccg tggttacaac caattgctac ttctcctaaa
      361 tagcgagaac cttcatctgt atctaagagg gtatggagta gttcttctcc tttttcagca
      421 gaggcttcta caaccttacc attttcaaag gttaatctaa tatcctctat ttcttttccc
      481 tggaagattc cggggtaaga aaatcttata tgtccttcgg ctgaatcttc gactggtccg
      541 gtgaacactt caccacctgg ataattttct tgacctttat ccgggatcca gattcttcct
      601 tccacattaa atttaatatc agtatctttt gatttataat gaagttcttt tacatcattt
      661 aaataatcgg catatccttc cagttcttta cctaattcct gccagtaggc aacagggtca
      721 tcttcatata agtggcaggc cttataaaca aaatcttcat actcatgtaa agacatcttt
      781 gcttcctggg catcggctga agtagggtat tgacagatag tccagtttaa gtcaccctca
      841 gctgctcttt tcatatgtat ttccattaat tctctattag ccaaactacg ggttttcatc
      901 ttctcgggat caacattagt catccctttc gtattataat gagctagtat cgaaattgaa
      961 gcatcaacat tttctactac atacttttga tagggtgatt gataatcaag ctggtgtttg
     1021 gaagcatatt caaagaatag ttcttcctgg ccctcaaaag ttggtacaac taaaggatga
     1081 gcgcctttct taagaatttt aatatataaa gctttaatta aaggttcagc caccatagct
     1141 cccttaatta aaacttgatc ccctttttct acatctacag aataatcaac aagtacatcg
     1201 gcatactttt ctaaaatcgg atttatcatt aatatccccc tttatttatt ccaaactaat
     1261 tgaaacatgg tcgccgccgt cattgatatc aactatttta cctttgctag attcatcatt
     1321 aatagcagat aagatttcat ctaaatttac attttgttct tccatctgct gctttgcctt
     1381 tttaggcata attttaagtg ctgttttggc taacaacata ggtatagaaa tattaacctt
     1441 ttcttgacca ttttcttcta ctaaaatttt aataaattta ggttttttac cacttgtttc
     1501 gggtttctca aatactgtat cccttttttc gagggttcct aatagttcat ttgcttcttc
     1561 agcatcgatt gttccttttt ctaacatctt taaaatttgt aatctttctt cactcacttt
     1621 tcatacctcc taattatatt tccccaagct gttcaacagc ttctgctgca gaaatttctc
     1681 cgctttctaa acgtgttaat acttcttttc tcttttccgc catttcatca tcggggctct
     1741 gctcaatatc atgccctaag gcatggatca cctcatctaa tttatttctg acagtgggat
     1801 aagagatacc gagttctctc tccacctctt taatatttcc gcgtgattta acaaagacct
     1861 ctataaactc caaatgttcc ttttttagtc tggaaaattt atctaattta aacttgccgc
     1921 tgattgttgt ttcacagctg ggacactgca attctgtaat gatcatatca tcaccacaga
     1981 ccggacagtt acctactaaa ttatgaatca caatttttcc tcctttctta atatattttt
     2041 aacatctacg ttactttcta ttcaatatta tattattaaa cttaataaat gtcaagtaag
     2101 atattaatat ttttaataat tatattaaaa tatttaatat ttacttcatt ttaccatagt
     2161 ttcctccccc accacttact atttttgctt ctccgcggcg ggccagatca attttttcag
     2221 ctaattggtc atttaatatc ttttttaatt cacttatggt agtatcatga ataacatcca
     2281 tttcagtatc aaactcttgt aaaagtttct gtaaagtttt tttaccaata cctggtatat
     2341 ccagtaaggg gatctgataa atatatttac ccctatattc agggtgtttg atctcttggt
     2401 cagaaatttc taatattcta tccttaaccc ctttaattaa ttccttacca caattcggac
     2461 agtaagaata gggtttttca tctgtaaatt tttgttgaca ggccggacaa aaggatcgat
     2521 ggtatttacc caatctggga tctaaaccaa aattagaaac gactcttctt ttatcttttc
     2581 ttaataaagt ctttttaaat tctttaaaat taagttcttg cattttaaaa atattatatt
     2641 cccgcgctat tttaggaata gaatgtgaat cagaatttga taaaaaggtc ttctctttta
     2701 actcactcaa gcggtctgcc aaatctgtat ctgcacttaa acccaattcg atagcaggaa
     2761 tcttatccca ttcttttttt gtgaaaactt tttgataact ttcaactgcg ttaccataaa
     2821 aacttttatg aggggtaaag gcatgggcaa taaccattat accgccggtc gcatcaataa
     2881 tttttagaat ctgagaacca gttaggccgg taacctggga actcaaattc acatttgtaa
     2941 tatactgttt cataatagtc gtaaattctt tgaggttatc taaaaagggg aaataggcta
     3001 agtaatgcac cataccaccg ttagcttccc ggctttccac ttcagctccg ggaataatta
     3061 caatctcatc ttgaaagtta aagcctcccc cgtctatctc ttttatctct cctttatcta
     3121 acatatcttc aatatcagcc tgaacccggg gagaagcaca gtctattatt ccgattaaat
     3181 cgagtccctt tctctcagaa ctctctctta caatattttc aaaggttaaa ctgttggaag
     3241 cggttatctt gaccggctca ccgtttttag aagccccaat gtggatgtgt aaatcagcaa
     3301 agtatctatt catctttggc ttccttattt aaataccata acagtccgat tatggtctta
     3361 ctatctttaa tttttccgtt taatatatta ggaatgattt cttcttcctc taactctaca
     3421 atttctatat attcacctaa atctaagttc tgtccataat gttttaaacc atcggcaata
     3481 aatagatgta gcttttcact actatagcct ggggtggtat aaaaatgaaa tagttctttt
     3541 atatcggcag gtctaaaacc ggtttcttct tctaattccc gggctgcaca tttttttaca
     3601 tcctcatcct cttctaattt accggccggt agttctaaga caacttcatc tacagctatc
     3661 cggtactgct taaccatcag gattttgccc tcttcagtca cgggcacaat tgcaactccc
     3721 ccactgtgtt caactacttc acgataagtt tcatggtcat tatatagttt aactttgtct
     3781 tttcttaagt ttaagattga accttcataa atatactcgc tctctagtat ttcttcttta
     3841 tatttatcca taattttcac tcacctctct gcttgttaaa tcactatcta ttttaacacc
     3901 ttagaacaat taaaaaaagc tcattcccgt gattaaggaa tgagcgtttt ttcattatat
     3961 tttaactatt tatactcttt gcctctatct aaagccttca cattcatatc aataaggtcc
     4021 tgattgcggg cagagagaac atcttctaga gactttttga cagtagccgg ttttactata
     4081 tctttttctt ctaagtacga tccgagcatt accatattag ctacccggga attaccgagc
     4141 tcatttgcta ttttatttgc cggtacttta attactttaa catcatctct ttcaacttct
     4201 ttttcgataa gtgaactgtt cataaagaca gttcctccac tttttacctg ggacaaaaac
     4261 ttatcgagtg aaggtaagtt catgactata atagtatccg gttttgaaga catcggtgat
     4321 ggtatctttt tatctgaaac aataacggta cagttggctg taccacctct catttcggga
     4381 ccataggaag gcatccagga aacttcttta tcttctttca ttcccgcata agaaagcagt
     4441 ttacccatta acattatacc ttgaccacca aaaccggcca taatgatttc ttcattcatc
     4501 ttactccacc ccctcaggtg attttaattc gcccagggga taagtttcga tcatattatt
     4561 tttcaaccac tcaattgcat cctgtggttt cataccccag ttagttgggc aggtagacaa
     4621 tacctcgacc agagcaaagc cttcgccgtc taactgcatt tgaaaggctt ttttaatcgc
     4681 ttttttagcc tgtctaatat gcttgggatc atggacagat accctggcga tataggcaga
     4741 cccatctaat tcggttaaca tttctgacat tttaatggga tgacctgctt cctttagatc
     4801 tctaccgtag ggagaagtag ttgtcttttg gccttctagg gaagtggggg ccatttgacc
     4861 accggtcata ccataaatgg cattattaac aaagatagta ctgattaact ctccccggtt
     4921 agcagcatgt acagcttcgg cagtaccgat tgaagctaag tcaccatcac cctggtaggt
     4981 gaaaacgaat ttatcgggat ggaccctctt aataccggtt gcaacggcgg gagcccttcc
     5041 gtgtgctgct tcatgcatat caacattaaa atattcataa gcaagcactg aacaaccaac
     5101 aggggcaact ccaatagtat cttctcttaa atcaagttca tcaataacct cagctacaat
     5161 tctatgaatt atcccatgtg tacagcccgg gcaataatga aatggtttat cagttaaagc
     5221 atctggatag cctgctactt gtttcattat ttttcacccc cgattttttc tatttcttct
     5281 acaatctctt cgggtgaagg tacgactcca ccagttctgc catagaaatg aactggtttt
     5341 ttaccgttaa cagctaaacg aacgtcttca accatctgtc cggtactcat ttcaactgct
     5401 aagaagttat caactctatc ggcagtttca tttataatat cagttgggaa tgggaacaat
     5461 gtgatcggtc tgatcattcc cactttatga ccatctttac gggccatttc aattgccctt
     5521 gatgcgatac gggcggttgt accataagcc acaataattg tatctgcatc atcggtgtaa
     5581 agttcttcat atcttacttc ttcttctttc attttgtcat atttagcttt taacttcata
     5641 ttatgatctt ctaaatcctg tggatctaag gctagagaat taatcacatt tggttctcta
     5701 cctttggcac catcagtagc ccattctttt ttaggtaaat cttccgggtc aaccgggtct
     5761 ttaaattcaa ccggttccat catttgtcca ataattccat ctcccactaa aagtacagga
     5821 tttctatact tatcagccaa atcaaaggct aacatagtta aatcaaccgc ttcctggaca
     5881 gaactaggag ctaggacagc taaactgaaa tcaccgtggg ccataccctt ggtagcctga
     5941 aaatagtctg cctgggaagg ctggattgaa cctaaaccgg gtcctccccg cataatattt
     6001 ataattactg caggtaattc agcccctgca atataggaaa ttccttctgc ttttaaactg
     6061 attccaggag aggatgaaga agttaatact ctcgctcctg ctccggcagc tccgtaaatc
     6121 atgttactag ctgcaacttc actttctgcc tgtaaaaatg ttccaccaac ttccgggaac
     6181 ttccttgcca gataggccgg caattcactt tggggagtta ttggatagcc aaaaaagtag
     6241 cggcagccgg ctctaatagc cgcttcagct atcgcttcat tccccttcat taaagttttt
     6301 tcacccatat atttaattaa tccccctttt tgttattctt ctttatacac tgatataaca
     6361 acatcaggac acatagttgc acacatgcca cagctgatac atttatcctg ttcttttacc
     6421 tgggcatagt ggtatccgtg actgttaata tcatctgcca tacttattat actaacagga
     6481 catacactta cacaaagttc acatccctta catctttcta tatcaaattc aacattttta
     6541 tgtttttctt tttccatcca gacaccccct tagtagttct ttgctttttc aattccggat
     6601 aatactcggt aagcaccggc cgccagggct tctaattctt cttcacctgc aaaaactttg
     6661 acaggtgaga aatgtctgac cctattttcg atcaggtgca caaaacggtc tgaataggca
     6721 ataccaccgg ttaagattat tacttcacag ttcccactta acacggcagc catactgccg
     6781 atttcttttg ctacctgata agccattgct tcaaatatta attgagcttt ttcatctcct
     6841 tggtcaattc tgttttcaac ttccagcata ttacttgtat ctagataggc tgccactcca
     6901 cctttaccaa tcaacttttt ctttatttct tctttttcat attcacctga gaaacacatc
     6961 tcaaaaaggt catagcttgg aactcctcca ctgcggtttg gactaaaggg tccatcacca
     7021 cttaaggcat tattcacatc gataatttta ccctttttat gggcaccaac tgatattcct
     7081 ccacccaggt gggcaactat tagattgata tctttatact ctttattaac tatttcggaa
     7141 tattttctgg caactgcttt ttgatttaaa gcatgtaaga tactttttct tcttagttca
     7201 ggtattcccg atatttttgc tatttcctgc atctcatcaa ctactacagg atccacgata
     7261 aaggcgggac agtctgcctt tttggcgagg aggtctgcta gcaatcctcc cagattgcta
     7321 gcatgttctc cccttttggc ttcttttaaa tcttctttca ttttttcatt tacgcgataa
     7381 gtaccaccat cgatcggtct aagtaatcct ccccgaccga caacagctgc gatgttttta
     7441 atttctatat cctctttttg caaaaattca aggatatagt tatatctaaa ctctaattga
     7501 tcacttattc tagcaaagtt ctttaaatct tttatatcgt gatcaatatt taattttttg
     7561 atttcagtgg tcctattata taatccaagc ttggtcgtgg tagacccggg atttatagta
     7621 aggattaaca tctccattgt gccactccta tcgagggata aagtttcttc ttactttacc
     7681 aatattatta attctttctt ctgccactat atcagcagct ttataggttg gaatatcgtc
     7741 tcttttacta atttcatata cctttttaat gttattatag atattctttg cacgtttaag
     7801 ggcacgttct ctatcataac cgattaattc atcatataca ttgataactc cacctgcatt
     7861 gataacataa tcaggggcat aaacgatacc tttttcttct aatacatcac catgtctttc
     7921 ttcttctaag acattattgg ctgcccctgc tactatatca cagtttaact gtggaatagt
     7981 atcatcattg ataacggctc ctaaggcaca gggggcaaag atatcacagt ctacactata
     8041 gatctttccg ggttctacaa ccctaacacc aaagtcttct actacttgat caatattttc
     8101 ctgtttaata tctgttacta ccacatcggc acccgcttct ttaagatgtt ttactaaata
     8161 gtagccgaca tgtccgactc cctgaacagc aacgacttta ccatttaaat catctgttcc
     8221 atatacttca tcagcagaag ctctaatacc gtggaaaaca cctaaagctg ttacaggaga
     8281 agggtctcca ctggttgatg gtaaaccggt cacataatcg gtttcttccg ccactatatg
     8341 catatcatca acggttgtat ttacatcttc agctgtaata tagcgtccat ttaggctctg
     8401 aacaaaacgg ccgtaagctc tccagagttc ttcgctctta atttctttgg ggtcaccgat
     8461 aataacggct ttaccgccgc ctaggtttag accggccgca gctgacttat atgtcatacc
     8521 tttggcaagt cttaatacat catttaatgc ttcttcctca gtatcataat cccaaattct
     8581 ggttccacct aaggcaggac ctaaagttgt gtcgtgaata gcaataatag cctttaaacc
     8641 agaagcctca tcataattaa atacaacctg ttcgtaatcg tctaattcca atctcttaaa
     8701 aagttcttcc attaataatc ttcctcccta aatattattt ttatcctctg tcattacctt
     8761 acccagtgca atagaattta atttagtttc ggctctatct gcccgggaag taatcacgag
     8821 aggtactttc gcaccaacaa caacactggc agaaggttgt tctccataaa acacaagtga
     8881 tttatataga atattgccag cctcgatatc aggcaccaat aaaatatctg ctttaccggc
     8941 aactggacta tcaatacctt tatgttcagc tgcctcttta ctgatcgcat tatcaaaagc
     9001 aagaggacca tctacagtta taccctcaat ctgtcctctg tcactcatct tggaaagcga
     9061 agctgcttcc agcgtagctt ccattgatgg attgaccgct tctacagctg ccaggacagc
     9121 acaaatcgga ttttcaacac cgatcgcatg ggccatccgg cctgcattat taatcagatc
     9181 aatcttatct tctaaagatg gactgatatt catgccgcca tcggtcatga tcacaaaacg
     9241 ttcttctttt tctaggtata ttaaagtaac caggcttaat atattttctc cacgtaatcc
     9301 atactgttta tccagaaggg cctttaaaat aatactggta ctgatatgac ccttcatcgg
     9361 atagtctgcc ctgccttctt taattagttg catggcttta tgggcagatt ttttttggga
     9421 agtagtacca actatttcaa ctttatcttt taaacctttc gcctgggcaa tctcatcaat
     9481 cttggcataa tccccgatta aaatcggctc aactatacct aaattagtag ctttttcaat
     9541 actatctata acaacttcat cagcagctgc tacaactgct aatttgaggg gcttagtttt
     9601 ggtagcttca tcaattagtt cttcaatgct ctctaacact ttactacccc cttatattat
     9661 gaaccgcttt catcatctca attctctctt caaccatatg gtcagctgcc tccacagttg
     9721 taattccttt ttcgtcggca acttcgaaga tttgtaatag tttattataa ataccttcac
     9781 attttttcat ggttctttct tcactatagc cgccgatacc cagttcatct gcaacctgga
     9841 ttaaaccacc ggcattaatt acataatcgg gtgcgtatag aatatctctt tcctgtagtt
     9901 tatgagcgaa atcatggttt tctaatacat tattggctgc tccagctact atatcacatt
     9961 ttaatctatc aattgtatct tcattgatta cagaaccgag ggcatttggt gagaagatat
    10021 cacaatcaac atcatagatt tcatcgggct ctacaatttt agcacctaat tctctgcctt
    10081 cttcaatata gtcttcattt atatctgtag cgaatacttc acaaccagcg tcgattaaat
    10141 gtttagttaa tttggagcca acttttccta agccctgtac tgcaactgta aggccgtcac
    10201 agtcatcttt tccatattta tacttgcagg ttgcctttat accgtgaaat actccataag
    10261 cagttgctat tgaagaatca ccgctaccac caaattcttt cggtaaagca ccaacataag
    10321 gagtttcttt tcttaaaatt acaaagtcat cataagtggt tccaacatca gtacctgtag
    10381 aataacgtcc gttaagagtg ttgatgaact tacctaatgc tctaaagatc ccttcacttt
    10441 tatctttgtt gggatctccc catataacgg ttttaccgcc tccaaagtca acacctgaaa
    10501 ttccagactt gtaggtcata ccctttgaaa gacgcagggc atcaataatc atgtcctctt
    10561 ctttatcata atcccacatt ctacaaccac cgagactggg gcctaaaaca gtgttatgta
    10621 ttgcaatgat tgctttgagg tttactttat tgtcctgcag gaacataacc tgttcatgac
    10681 catgtttttc catttcttta aatagttcca ttaaaaatat ttcccccttt aagtaagatt
    10741 aattttgcca ccatatatat tatgcatatt ccgttccaag taattattat cagcattatt
    10801 ttttatgtaa agcctgtata ggctgcagga ataggatttg ttaaaaaagt aaaagttttg
    10861 ctaatatttt cacaactgca aaataaaaca tgcctgcaac ttaatgcagg catgtttttg
    10921 cagtttttat gcttttttgt taatcaatct catatttatt gatcttataa taaaggctcc
    10981 ggacggcaat tcccagtatt tctgctgcct cttttcgatt ccctcccgtt tctttaaggg
    11041 ctttttggat aacctgtttt tccgttctat ttacgatatc tttgagggaa ttactatcaa
    11101 gagaatactg agcacccgac ttaacgagat cttcagcttc ttctgaagca cccgtgatat
    11161 ccgggatgtg ttctatctca attatatctt catttaagcc taagtttatc atggccctcc
    11221 cgataatatt ttcaagttct cttacattac cgggccattc atacttttta agatagttaa
    11281 ttgcttcctt ggaacaaccc ttgacagacc gtccatattc ctgattaaac ttggtaatta
    11341 tatggtggat taaaaaaggt atatcttttt tcctttccct taagggtggg ataaagatcg
    11401 gaaagacatt taaacgataa taaaggtctt ccctaaactt accatcttga atggcttttt
    11461 ctagattgat attggtggcc acaataattc gcacatcaat atcaatcggt cgggtaccac
    11521 ctactcgcag gatttccttt tcctgaataa cccttaagag tttagactgt aagttcatgc
    11581 tgatttttcc tatttcatct aaaaagatag ttccctgatc tgcttcttca aagagacctt
    11641 ttttaccacc ttttttagcg cccgtaaagg caccttctgt ataaccaaaa agttcactct
    11701 ctaagatatt gtcagctagg gcagaacagt ttactcgtac gaacttctgc tgactgcgtt
    11761 cactattatt atgaatagcg tgggcaaaaa gttctttacc ggtaccgctt tctcctctta
    11821 acaggaccgt aatcggcgtt ttagaggcct tttcggcctg attgattaca gcctgcatgg
    11881 tgtccgattc acccacaata tcctgaaatg aatacttggc attaatctgt cttatgatct
    11941 gtttggcctg gtctaattcc tcggttagtt ttttgatctc ggaaacatca tgggcaaccg
    12001 ctacacttcc cttgagctct ccatctacta tgattggtgc agcatcaact attacatctt
    12061 tcttattggg gccgactttc atcctgaccc ctttaaccgg tttttccgtc tctaagacct
    12121 tgtaatgcat actccgtcct tctgcaatat cgacattggc cggtttacct attatatctt
    12181 cttctgttaa gccggttaat ttcgtatagg ccgggttgat taaaataccc accccgtcct
    12241 catcaaccac actgatcgca tcctgtgtgg aattaatgat ggcttccagc atactcctga
    12301 tctcgcgtag attagtaatc tcttccgcta aatttttaac ttccgtgata tctctaaata
    12361 cggcgaccgc cccaataata ttttcttctt tgtccttaac aggtacccgg ttagttatga
    12421 tggttgtttc atgggtgtgt tggatttggt tcagttcggc ttccccgttg acaaggacat
    12481 ggtgcagtct tgtattaggg attacttcct ctactttttt atttaatact tcttttcgat
    12541 ccagggcaaa catattttcg gccgcatcgt taaaaagctc aattcgtccg ttttcatcta
    12601 cggaaatcag accatcatgg gtagaattta ttataacgtt cttttttcta attatatttt
    12661 taagttcctt aattcttgcc tctttatttt tcttactcat attttcactc cagctggaac
    12721 ttaatcccag ggatttttga ggaagcgctt aattgtaaag atctcttcct cataatctaa
    12781 cttctctaat tgccccttta aatcctctct cacagagata aatttaatag acaggttaag
    12841 ttcttgggca gcctctttca cgagaggata accattttta acatcttcta tggttgtctc
    12901 ttcccccagg tttatattac agaccaaatt gtcaacatct aacttagaag ctgactcaat
    12961 tttttctttc atctttatga taccctctac atcttctgta aaaggcctat tggtgttaat
    13021 tactagattt aattcatagt ctataccccg cacatcatcg gcaatactgc ctaaaacagt
    13081 agcaccgtct tcttccccac cgacatcaat cacaccatga tactcatcat tttgtaacag
    13141 ttttttgata tccggtgtta ttatgggtag atcagcatac tttaattcct tggggtaaat
    13201 tacttctata tcttctgctt ccatcaattc atttttttct ctggagcgaa aatagggatt
    13261 aattgtatct agatctataa ctacaacctt attattcttt tctctaagat gtagcgcgta
    13321 atttaaagaa atttcagtct taccgcttcc gaaatttccc gtaaaaattg ttactctttt
    13381 atcaatcatt atatttattg attccccaat gtagcaacca taacagcctt tattgtatgc
    13441 attctatttt ctgcttcatc aaatgcctta gaatacttgc tctcaaatac ttcatctgtt
    13501 acttccatct cttttatacc gtattcttca tatatttttt gaccgttttc agtatcggta
    13561 ttatggtaag ccggtagaca gtgtaagaag attacatctt cattacctgt attttcgatc
    13621 atttccatat taacttgata tggttttaac atttcaatgc gttctgcaaa cttctcttct
    13681 tcccccattg aaacccatac atctgtataa atagcgtcag catctttaac acctttgaca
    13741 ggatcatctg taatttcaat ggttgctttt gaatttttag ccagttcttt tgctttatcc
    13801 actaactctt gtttcggcca tagttcttta ggggctacaa ttctataatt taagcccatg
    13861 atagcactac ccatcattag agagttagcc atattattac gtccatctcc tacatatact
    13921 agatttatgc cttctaatct atcgaagttc tcttcaatag ttaaaaagtc agctaaaatt
    13981 tgagtaggat ggaactggtc ggtaagtcca ttccagaccg gaactcctgc atattccgct
    14041 aactgttcca cggtttcatg tttaaatcct ctaaattcaa taccatcata gtatctaccc
    14101 aatacacggg cagtgtctgc tacagtttct ttttttccaa gttgaatatc attttttcct
    14161 aaatactccg gcataccgcc ctcatcttta gcagcaacag aaaaagaaca tcttgttctg
    14221 gtggaaggtt tttcaaaaat taaggcaatg tttttacctt ccagagtttt accggttaaa
    14281 ccagttcttt tttgatcctt taagttcttg gccaaggtca ataaatagct gatttcttct
    14341 ttcgtaaagt cctgtaatga taataagctt cttcctttga gattaaatgc catcttatct
    14401 cccccatatt attattttta tttatcacac atttcatatt atataataag tgaggtaaat
    14461 attcaataat ttttatacaa ctttgaatat ttattcaatt agttattaat ctattcctca
    14521 gtctaaaatc atcggccacc ttggctataa actgagcatt tgtaggacgg ccgattgttt
    14581 ctgaaatgct gtagccaaag tattcggata tcttgtccct attaccgcgc tcccaggcta
    14641 cttttattgc atgtcggata gccctttcca ctctactggc tacagttgaa aagttctcgg
    14701 caattttagg atataattgt ttagttatag aattgactaa ttccaggtct gttatactta
    14761 aataaatagc atttcttaaa tacatataac ccctgatatt agcgggtacg ccgaactcat
    14821 ctaacaaacc ggtgatctct aaattcatat ttacttcatt ttcatcactg tttaactgct
    14881 cagccaaact gctttcgtaa ttagataatg tataatcttg atctaggtcg gtaatcctat
    14941 ttttaataca gtcaaaatca gctggttttg acataaagta gtcgatttca tagttctgca
    15001 ggattttaag catctggtca tttaaaaagg atgataaaac aataatattc acatcctcta
    15061 ttttctcctc ttctcttaac tcatccagga cacttacccc atctagataa ggtaaaatta
    15121 gatccataag cacatagtcc gggtcttccc tgaagatcaa ttgtaacccc tcatgtccat
    15181 cagttgctgt ggcagcacat gcaaaatctt ctgtttgatt gagttctgtt tcgaactttt
    15241 gacaaaattc ctcgtcttga tcaattatta cgacctcttt tacatcagtt tccataaatt
    15301 tgattcctcc taaaaattat taataaaaat gaaattttat tgtattgtag tgtagtgttg
    15361 attagttttc ccttataata gttacaataa aaaggaagaa tctccttcta aatttggaaa
    15421 tattttaaaa tttagtaaaa attaataaag cagccagtat tattttgaaa ctttacctaa
    15481 atactctgct gcttttcttc agccatttta agcatctcgc gggcatgcct tttggtagta
    15541 tcggttaact taaccccacc taacatcctc gcaagctctt ctatggtttc tttttcattt
    15601 aattttttaa tattggtaac ggtctttttc tcctcattag tttctttgtt tatatagtaa
    15661 tggttatcac ccatactggc cacctgaggt agatgagaaa cacagactac ctgtctttta
    15721 ttggaaattc gatagagctt ttcagctagc ttttgggcag ttttaccccc tattccctta
    15781 tcaacctcat caaataccaa tgtttcgacc cgatcgatat cggccataat atttttgaag
    15841 gctaacatga tccgggacag ttctcccccc gaaattattc tactcaaagg tttaagatct
    15901 tcgccggggt tagtagaaat caaaaatttt acatcatcta taccttcagc cgtccgcttc
    15961 gcttttgcga actttacttt taacctcgct ttttccatgg ccaggtcttt tagttcgttt
    16021 tttaaatctt cctcaaattt ttcggctgcc tcttttctta tatcagataa ttcttttgct
    16081 tttttgtcat attggttctt taatttactt aatttgtttt ctagttcgtc gataatcttt
    16141 tcttgatttt ttagctccga taacttttct tttaagtcat ctttatattc taaaatctcc
    16201 tcgattgaat ctccatattt gcgctttaac ctgttaatca agtccagtct ttcctc
//
LOCUS       DATGCJ010000080         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_412749, whole genome shotgun sequence.
ACCESSION   DATGCJ010000080 DATGCJ010000000
VERSION     DATGCJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412749"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VKN64_04200"
     CDS             <1..1282
                     /locus_tag="VKN64_04200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMA59473.1"
                     /translation="LFFYLGAFSPLQDELEKTLLENFKNTVSIAEINVENYISRAKEG
                     AESLSSRTMIKSMLNKFNRGDISYLEVKQYTQPKYSDGAKVLDHVIKAVRFCNGEKLI
                     AGCDGISGDKSIDMDLKEFNQDVKKTKIYILHKKNLVIINSPIILEQEKLGNDIVVFD
                     LKPLFSNLNKERINYSIFASNEVPNKFVHNNKEVIDYRKILDTNYYLRAQLPQTQLNE
                     GINELLRRVIIGIITALIIITILFRIIIKQTYHKVIDRLKNKIDKITKLSETDYLLKI
                     YNRSKFKDELTKEISRVKRYGSNLSLIMFDVDYFKKINDEYGHDKGDDVLIKITEIIK
                     DIIREADVFARYGGDEFIILTPNTDLKEAGQLAERLRKEVAESEFDQIGSVTCSFGVT
                     EFGSDDDIDSFIKKVDDALYKSKEKGRNKVEKKK"
     gene            1381..>2120
                     /locus_tag="VKN64_04205"
     CDS             1381..>2120
                     /locus_tag="VKN64_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HMA59474.1"
                     /translation="MENKYHKIDNLDKLQPGDHTVLIYEKEEDTHSSLVSFIRASLLR
                     GEKCFYIEGDTNTEFLIKTLKKNMENFTQYIDSSQVQFLTKEETYALSKEFKADKMIK
                     MIKDLTETALEEGYTGLSITGELSWVLNFEGGKEEIIEYEWKLNERVFNEYPVVALCR
                     YNLNKFDQDIIKAVLELHDYIIWQDRIHENPYYIEPDGYKNNDVVDYEIKAWLENIQK
                     YKKRESVFKERLEEKESEYEFLFNQISDA"
BASE COUNT          849 a          243 c          400 g          628 t
ORIGIN      
        1 cctctttttt tatttagggg cattttctcc tttgcaagat gaattagaaa aaacccttct
       61 tgaaaatttt aaaaatacag tttctattgc agaaataaat gttgaaaatt atataagtag
      121 ggccaaagaa ggagcagaaa gcttatcaag tcgaaccatg attaaaagta tgcttaataa
      181 atttaatcgg ggcgatataa gttatcttga agttaagcag tatactcaac ccaaatactc
      241 agatggagca aaagttttag atcatgtcat aaaggcagtt agattttgta atggagaaaa
      301 actaatagca ggttgtgatg gtatatcagg tgataagagt atcgatatgg atttaaaaga
      361 gtttaaccaa gatgttaaaa agacaaagat atatattcta cataagaaaa atcttgttat
      421 tattaattca ccaattatat tagaacagga aaaattaggt aatgatatcg ttgtatttga
      481 tttaaaacct ttattttcta atttaaataa agaaaggatt aattacagta tatttgcaag
      541 taatgaagtg ccaaataaat ttgttcataa caataaagag gtaatagatt acagaaagat
      601 tttagatact aattattatc tcagggccca actgccacaa acacaactta acgaaggtat
      661 aaatgaactc ttaagaaggg tcataattgg aataattacc gcattaatta ttatcacgat
      721 attattcagg ataattatta agcaaactta tcacaaagta attgatagac taaagaataa
      781 gatagataaa attacaaaac tttctgagac agattatctc ctaaaaatat ataaccggtc
      841 taagttcaag gatgaattaa caaaagaaat cagccgagta aaaagatatg gtagtaatct
      901 atctttaata atgtttgacg ttgattactt taaaaagatt aatgatgaat atggtcatga
      961 taagggtgat gatgtattaa taaaaattac tgagattatt aaggatatta ttagagaggc
     1021 agatgtattt gctcgttatg gcggggatga gtttattatc ctaactccaa atacagatct
     1081 caaggaagcc ggacaactgg cagagaggct aagaaaggaa gtagcggagt cggagttcga
     1141 tcagattggt agtgtgactt gtagttttgg tgtgactgag tttggaagcg atgatgacat
     1201 tgattccttt atcaaaaagg ttgatgatgc cctctataag tccaaagaaa aaggtagaaa
     1261 taaagtggaa aagaagaaat aacataaata tcccagatca ggtattatat atgttattca
     1321 agacaattaa tagtaaaata aaaatacttt cctcaaaatt attttggagg ttaaaaatat
     1381 atggagaata aataccataa gatagacaac ttagataagc tacaacctgg tgatcacaca
     1441 gtcttaattt atgaaaaaga agaagataca cattccagcc tggtttcttt tattagggca
     1501 agtttattac gtggggaaaa gtgtttttat atagaaggag ataccaatac agagttttta
     1561 atcaaaactt taaaaaagaa catggaaaac tttactcagt atatagatag ttctcaggtc
     1621 cagtttttaa caaaagaaga aacctatgct ttgagtaaag aatttaaagc agataaaatg
     1681 attaaaatga taaaggatct aacagagact gctttagagg aaggttatac tgggttaagt
     1741 ataactggag agttaagttg ggtattaaac ttcgaaggcg gcaaagaaga aataatcgaa
     1801 tatgaatgga agttaaacga aagggtattt aatgagtacc cggtagttgc tttatgtaga
     1861 tataatttaa ataagtttga ccaggatata attaaagcag ttttagaact gcatgattat
     1921 ataatttggc aagatagaat ccatgaaaac ccctactata ttgaaccgga cggttataaa
     1981 aataatgatg tagtagatta tgagataaaa gcctggcttg aaaatatcca gaagtataaa
     2041 aaaagagaaa gtgtattcaa agagagattg gaagaaaaag aaagtgaata tgaattcctc
     2101 tttaaccaga tcagtgatgc
//
LOCUS       DATGCJ010000081         5570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_458043, whole genome shotgun sequence.
ACCESSION   DATGCJ010000081 DATGCJ010000000
VERSION     DATGCJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458043"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VKN64_04210"
     CDS             <1..544
                     /locus_tag="VKN64_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HMA59475.1"
                     /translation="WPDRADVPRNAARALRYMDEYVTVKQGEVFYITEALAQIEGMER
                     GPAGNTSLAAAFDLAQKMDEDEIVVVQETEYTGAGKHPMAQLNFAKENGIDVRNGDPD
                     EEEAGKSVIIPEHPSQIKARDVDLEKLKRSYLRKVIEDTNVKEIEDIDLEFLADEIKK
                     SKAETKEILEEEFDLTVHGG"
     gene            548..1882
                     /locus_tag="VKN64_04215"
     CDS             548..1882
                     /locus_tag="VKN64_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HMA59476.1"
                     /translation="MKVDLLIAEIGSTTTLVNAFDNIDSANPDFIGQGFAPTSVLEGD
                     VTVGLKGAISDLEKKKGIPKLNWEEFMATSSAAGGLKMTVHGLVKDMTVKAAEEAALG
                     AGAVIKQVTAGKLRDSQLEELRQIQPNIILLAGGVDYGEEEVILDNAEKLSSLDIDVP
                     YIYAGNKVLQNEVKELFEKKGKHIIIADNVYPEIDTLNIEETRNIIHDVFAEHIVRAP
                     GMSKIKPMLSIDMMPTPGAVMKASQLLKDDIGNLVTIDVGGATTDVHSVTEDSLNVRE
                     LLISPEPEAKRTVEGDLGVYLNAPHVYNLLDDRIDLEKREDLAPVPRDKEEERYIFHL
                     AKQAALTAIERHSGKYRDLYGPSGRSTVAEGKDLTAVKWIIGTGGALTRLTDSKQILE
                     SLKDFNKRALLPPKSAEALIDRDYIMASMGLMSKKYPKAALTLLKASLDFKN"
     gene            1912..2286
                     /locus_tag="VKN64_04220"
     CDS             1912..2286
                     /locus_tag="VKN64_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HMA59477.1"
                     /translation="MPIKREDDFEERRSHLKDLSDEELKERFWELAEQIVDPLIDLAD
                     THTSASIERSVLLRMGFNSVDAKAIVNQVVKADLLSKGAGHVVYKLAQKEDRPVKEVG
                     LDIRDEKYEKEELQALFDGGEK"
     gene            2286..4490
                     /gene="oraE"
                     /locus_tag="VKN64_04225"
     CDS             2286..4490
                     /gene="oraE"
                     /locus_tag="VKN64_04225"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HMA59478.1"
                     /translation="MDLKPEKKLDIKEILKDIENYRPRRKGWTWRNKVEDQEIGPFEY
                     KETSERLDNSVPLPAARSFSNIDPQPEPVITTEIASGRFEDDIRRMRMAAWHGADHIM
                     VIRTAGQSHFDGLIEGTPEGVGGIPITRKQIRATRKALDYIEDEVGRPINFHSYVSGV
                     AGAEIAVLFAEEGVNGAHQDPQYNVLYRDVNMYRSFVDAAVAKKIMTSTDMVQIDGAH
                     NANATARQAWKVMPELFVQHAINSMFSVKAGMPKDKISLSTVPPVAPPAPDLRLNLPY
                     AIALRELFDEYKFRAQQNTKYMESSIREATVTHTLNLLLSELTSADLQSTITPDEGRN
                     VPWHYFNVQAVDTAKQAFAGMDGLKELVELKTEEGYLKDKSRELKERAVLFMEEILDV
                     GGYFKAVSEGFFVDSGEYPERNGDGIVRQLDGGVAANSIVPRDDDYMAPVCDHFGYNN
                     LPDGLDKPCDLIGGCTLHTPEKIDFIDELDPEDNVEKRLENTKDFRENDKIRPEVQWL
                     GDGWVNVTMFIPEEETIAEYAALEIAKKMNLNKPEVIHKEVMQPAEGTLCEVKGQVDF
                     SVDKNELEIPEKEETLPYDDIRGFVDENPISVVAATVGKDEHSVGMREIIDIKHGGIE
                     KYGIDVTYLGTSVPISKVVDAAIETNADAILISTIISHNEVHRTNMKKLNDLCVEKGI
                     RDDVILIAGGTQVNNDMAIKAGLDAGYGRGTHGDDVATFLVKTLRGDYDEKK"
     gene            4477..>5570
                     /gene="alr"
                     /locus_tag="VKN64_04230"
     CDS             4477..>5570
                     /gene="alr"
                     /locus_tag="VKN64_04230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HMA59479.1"
                     /translation="MKKSKLTRPVWAEVNLDNIKFNLNQVKKNVPEETLIMAVVKADA
                     YGHGVLPVAEAAVEAGADRLAVALPEEGRELRKADYELPIQILGEVLPEQVSILVDND
                     LIPTISKLETVELLNELAGKKDIIKKVHVKVDTGMGRIGVFPDNAVDFIKEVMNFENI
                     EVEGLMTHFAKADEEDKDYTYNQWDQFQMVIDRLAEENIEIPIKQAANSATIIDLPHM
                     SLNMVRPGIMMYGLRPSHEVDQDFKLKPALSWKAKIVYLKEVPPGTGISYGAIYITEK
                     NAKIATIPMGYADGYSRLLSNKGEVLIKGQRAPIRGRVCMDQFMVDVTDIDDVKIGDE
                     VVLIGKQGDEEFSATEMADIIGTINYEITC"
BASE COUNT         1923 a          923 c         1288 g         1436 t
ORIGIN      
        1 ctggccggat agagcggatg ttccacgcaa tgcggcccgt gctttaagat atatggatga
       61 atacgtaacc gttaaacagg gagaagtctt ctatattact gaagcactag ctcaaattga
      121 aggtatggaa agagggccag ccggaaatac ttccctggca gccgcctttg atttagctca
      181 gaagatggat gaagatgaaa tagttgtagt acaggagact gaatataccg gagccggtaa
      241 acatcctatg gcccagctta actttgctaa ggaaaatgga attgatgtta gaaacggaga
      301 tccggatgag gaagaggccg gcaagtcggt tatcattccc gaacatcctt cgcaaatcaa
      361 ggctagagat gttgacttag aaaaacttaa gagatcctat ttaagaaaag taatcgaaga
      421 tacaaatgta aaagagattg aagatattga tcttgagttt ttagctgatg agattaagaa
      481 atcaaaagca gagaccaaag aaatattaga agaggaattt gatttgaccg tacatggagg
      541 ttaaaagatg aaagtagatc tattaatagc tgaaataggc agtactacta ccttagtaaa
      601 tgcttttgat aatatagata gtgcgaaccc tgattttatc ggtcagggtt ttgcaccaac
      661 ctctgtttta gagggtgatg taacggtagg attaaaaggc gccatttcag atttagaaaa
      721 gaaaaaggga ataccgaaat taaattggga agagtttatg gctacaagca gtgctgccgg
      781 tggacttaaa atgaccgtcc acggccttgt aaaagatatg actgtcaaag ccgctgaaga
      841 agcagcttta ggggccgggg cagttataaa gcaggtcact gctggtaagt tgagagattc
      901 acaattagaa gaattaagac aaattcaacc taatataata ctattggccg gtggagtaga
      961 ttatggagaa gaagaagtaa tactagataa tgccgaaaag ttgagttcgc ttgatatcga
     1021 tgtaccctat atttatgcgg gaaataaggt tttacaaaat gaagttaaag aactttttga
     1081 aaaaaagggt aaacatataa tcatcgcaga caacgtttat cccgagattg ataccttaaa
     1141 tattgaagaa actagaaata taatacatga tgtttttgct gagcatattg taagggcccc
     1201 gggtatgtct aagattaaac cgatgttatc gatagacatg atgcctaccc ccggagcagt
     1261 gatgaaagcc tcccaactgc ttaaagatga tattggtaat ttagtaacca tcgatgtggg
     1321 aggagccaca accgatgttc attcggtcac cgaagattct ttaaatgtga gagaactttt
     1381 aattagccct gaaccagaag caaaaagaac cgtggaaggt gatctgggtg tttatctaaa
     1441 cgcccctcat gtatataact tacttgatga tagaatagat ttagaaaaaa gagaagacct
     1501 tgcccctgtt ccgcgcgata aggaagaaga aagatatatc ttccatttag caaaacaggc
     1561 tgccctaacc gcaattgaaa gacattccgg taagtaccgc gacctttatg gtccgagcgg
     1621 ccgttcgact gtagcggaag gcaaggacct tactgctgta aaatggatta ttggaaccgg
     1681 tggtgcgcta acaaggctta ctgatagtaa gcagatactg gaaagtttaa aagattttaa
     1741 taaaagagct ttactgcctc ctaaatcggc agaagcccta atcgaccgcg attacatcat
     1801 ggcatcaatg ggtttaatga gcaaaaaata tccaaaagca gctttaactt tactcaaagc
     1861 aagtctagat tttaaaaatt agagcatttt ttaaggaaag gaagtgatct gatgccgatt
     1921 aaaagagaag atgactttga agaaagacgc agtcatctta aagatttatc ggatgaagaa
     1981 ttaaaagaaa gattttggga actagcagaa caaatagttg atcctttaat tgatttagca
     2041 gatacacata cttctgcttc tatcgaaaga tcagtcctac tgagaatggg ttttaacagt
     2101 gttgatgcaa aagcaattgt taaccaggta gtaaaggcag atcttttaag caagggagca
     2161 ggtcacgttg tatataagtt ggcccaaaaa gaagatagac cagtcaagga agtcggttta
     2221 gatattagag atgaaaagta tgagaaagaa gaactacagg cactatttga tgggggtgag
     2281 aagtaatgga tttaaaaccg gaaaaaaaac ttgatataaa ggaaatactc aaagatatag
     2341 aaaattaccg ccctcgtcgt aaaggctgga cctggcgtaa taaggtggaa gaccaggaga
     2401 taggcccttt tgaatataag gagacttctg aaaggttgga taatagtgtt ccactaccgg
     2461 ccgccagaag ttttagtaat atcgatcccc aaccagaacc agtaattaca accgaaattg
     2521 cctccggtcg ttttgaagat gatattagaa gaatgagaat ggccgcctgg catggagcag
     2581 accatatcat ggtcattaga actgccggtc aaagccactt tgacggccta attgaaggaa
     2641 ccccggaagg tgtgggggga attccaatta ccagaaaaca gattagggcc acccgtaaag
     2701 cccttgacta tattgaagat gaagttggcc gtcccattaa cttccactct tatgtaagtg
     2761 gagtagccgg ggcagagatc gccgtattat ttgccgaaga aggtgtaaac ggggctcacc
     2821 aggatcctca atataatgtg ttatacagag atgtaaatat gtatagatcc tttgtagatg
     2881 ctgccgtagc caaaaagata atgacctcta ccgatatggt gcagattgat ggggcccata
     2941 atgcaaatgc gaccgccaga caggcctgga aagtcatgcc tgaacttttt gtccagcatg
     3001 cgataaactc catgttttct gtcaaagctg gtatgcccaa agataagatt tctttatcga
     3061 cagtaccacc tgtggcacca ccagcaccag atttaagatt gaatctacca tatgcgatcg
     3121 ccttaagaga actatttgat gaatataaat ttagagccca gcaaaatacc aaatatatgg
     3181 aatcttctat tagagaagcg accgtaaccc atactttaaa cctattatta tcagaactaa
     3241 catcagctga tctacaaagt accattactc ctgatgaggg tagaaatgta ccctggcatt
     3301 actttaatgt gcaggccgta gatacagcca aacaggcttt tgctggtatg gacggactaa
     3361 aagaattagt tgaactaaaa accgaagaag gttacctaaa agataagtcc agagaattaa
     3421 aagaaagagc tgtactcttt atggaagaga ttcttgatgt cggcggttac ttcaaagcag
     3481 tatcagaagg attctttgta gactccggtg agtatccgga aagaaatgga gacggaattg
     3541 taagacagct agatggtgga gttgcagcca attcaatagt tccacgtgat gatgattaca
     3601 tggctcccgt ctgtgaccac tttggctata ataatctgcc agatggttta gataagccct
     3661 gtgatttaat cggtggctgt acattacaca cacctgaaaa gatagatttc atagatgaat
     3721 tagacccaga ggataatgta gaaaaaagat tagaaaacac aaaagacttc cgggaaaatg
     3781 ataagatcag accagaagtg cagtggctgg gtgacggctg ggtaaatgta acgatgttta
     3841 ttccagaaga agaaaccatt gcagaatacg cagctttaga gatagccaag aagatgaact
     3901 taaataaacc ggaagtgatt cacaaagaag ttatgcagcc ggctgaaggt accttatgtg
     3961 aagttaaagg tcaggtagac ttctcagtcg ataaaaatga attagaaatt cccgaaaaag
     4021 aagagacctt accatatgat gatattaggg gtttcgttga tgaaaaccca atcagtgttg
     4081 tagctgcaac cgtaggtaaa gatgaacact ctgttggtat gagagagatc attgatatca
     4141 agcacggtgg aattgaaaag tatggtatag atgtaactta tctcggaact tctgtaccga
     4201 tttctaaagt tgtagatgca gctatagaaa caaatgctga cgcgatttta atcagtacaa
     4261 taatcagtca taatgaagtt catagaacta acatgaagaa gctaaatgac ctttgtgtag
     4321 aaaaagggat cagagatgat gtaatcttaa ttgccggtgg tacccaggtt aataatgata
     4381 tggctataaa agccggtctc gatgccggtt atggtcgcgg tactcacggt gatgatgttg
     4441 cgacattctt agtaaaaaca ttaagagggg actatgatga aaaaaagtaa acttactaga
     4501 cccgtttggg cagaagtaaa cttagataat atcaagttta atcttaatca ggttaaaaag
     4561 aatgtacctg aagagaccct gataatggca gttgtaaaag ctgatgccta tggacatggt
     4621 gtcctaccgg ttgcagaggc agctgttgag gcaggcgctg acagattggc agttgctctg
     4681 ccggaagaag gtagggaact tagaaaagct gattatgagc tccctatcca aattttaggg
     4741 gaagttttac cggagcaggt ttctatctta gttgataatg atttgatacc cactatttca
     4801 aaactagaaa cagttgaatt actaaatgaa ctagctggaa aaaaggatat tataaaaaaa
     4861 gtacatgtaa aagtagatac aggtatggga aggattggcg tctttcccga taatgcagta
     4921 gattttatca aagaggtaat gaactttgaa aatattgaag ttgaagggct catgactcac
     4981 tttgcaaaag cagatgaaga agacaaagac tatacttata atcagtggga tcaattccag
     5041 atggttatag accgattagc agaagaaaat attgaaatac ctattaaaca ggcggctaat
     5101 agtgctacaa tcattgatct acctcatatg tccctaaata tggttagacc cgggatcatg
     5161 atgtacggtt tacgcccttc acatgaggta gatcaagact ttaaattaaa gccggcctta
     5221 agctggaaag ctaaaatagt gtatctaaaa gaagtcccgc ctggaactgg aattagttat
     5281 ggtgcaatct atattacaga aaaaaatgca aaaatagcta cgataccgat gggttatgcc
     5341 gatggttatt cgagacttct ttccaacaaa ggggaagttt taattaaagg ccagcgtgca
     5401 cccattcgag gtagagtatg tatggaccaa tttatggttg atgtaacaga tattgatgat
     5461 gttaaaatag gagatgaagt tgttttaata ggtaagcaag gagatgaaga attttctgcg
     5521 acagagatgg ctgatataat cggaacaata aattatgaaa ttacctgtga
//
LOCUS       DATGCJ010000082        25360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_368403, whole genome shotgun sequence.
ACCESSION   DATGCJ010000082 DATGCJ010000000
VERSION     DATGCJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 25360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25360
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368403"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VKN64_04235"
     CDS             complement(<1..953)
                     /locus_tag="VKN64_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA59480.1"
                     /translation="MESHYNFEPIEGKWQKDWQEKSLYKTKNKDEKDNYYVLEMFPYP
                     SGKLHMGHVRVYSIGDVIARFKRMKGYNVLHPMGWDAFGLPAENAAIKHGNIHPREWT
                     WENIESMKEQMRAMGLSYDWDREVATADPDYYKWTQWFFVKMFENDLAYRKEQAVNWC
                     PSCDTVLANEQVVNNSCERCGTEVEQKELEQWFLKITEYADELLEGHDKLDWPERVKT
                     MQKNWIGRSEGMSINFPIEEIGEELEVFTTRPDTIYGATYMVLAPEHPYVDELISGKE
                     NEDKIRDFIKKVQKQKEMERTSPESEKEGIFTGSYATNPMTG"
     misc_binding    complement(1012..1220)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1269..1631)
                     /gene="rsfS"
                     /locus_tag="VKN64_04240"
     CDS             complement(1269..1631)
                     /gene="rsfS"
                     /locus_tag="VKN64_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMA59481.1"
                     /translation="MPEENYKRIAELAAEAADDKKAFDIEILNVEGLTIIADYFVLCS
                     GNTDKQVQAIARAVEEKLDEEGVYPKQIAGKDNGRWILMDYADVIVHIFHKREREYYE
                     LDRLWADAKKILKKEETS"
     gene            complement(1635..2807)
                     /locus_tag="VKN64_04245"
     CDS             complement(1635..2807)
                     /locus_tag="VKN64_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HMA59482.1"
                     /translation="MLERLKNINWKIVGIFLLIVIISLSLYILLNDEFSLVNLNPFRK
                     SKMNILIAGYDSSINGPPRADTIIVASVNLDTNNIGLLFIPRDTRVKVNGHGYDRINA
                     SHAYGGIDLLKETVEDFLNIKLDYYVETDFQGFAEIINLIGGIDITIEKPLHYVDKAG
                     GLYIDLPAGNVHLNGEKALEYVRYRGTRGDIGRVARQQKFIQAVMEKAVSSKVLLNLP
                     SIYKEVMDSVNTNIPLKDVTPFAKLAKKTNLNNLKTEMVPGRPEYINRASYWIPTEED
                     LKIMVNNLIRSKEYIQNDQYQITLLNGNGVSGVAGEAAEELEKFGFNIKEVANAKDFN
                     YQETIIYYQNGNKKVARGLKDVMGGKIEKNEEIEVDFQIILGQNFVENQIAKKGGN"
     gene            complement(2808..3374)
                     /gene="yqeK"
                     /locus_tag="VKN64_04250"
     CDS             complement(2808..3374)
                     /gene="yqeK"
                     /locus_tag="VKN64_04250"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HMA59483.1"
                     /translation="MVEIKKLKEKLKKTKNFRIKHTQAVEETALKIAKRYPVEIDKVK
                     IASLLHDFARHYEDEKLLKMVEENNIEVDDLEKEIPALLHSPVGAYLAEHKFGIKDED
                     ILNAIRFHTIGRTGMSRLEKIIFVADVIEPGRNFPGVDLIREKIDKDLDEAVILVCDF
                     TIKYNIDRKRVIHPNTLYTRNSLLKGAK"
     gene            complement(3380..3973)
                     /gene="nadD"
                     /locus_tag="VKN64_04255"
     CDS             complement(3380..3973)
                     /gene="nadD"
                     /locus_tag="VKN64_04255"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HMA59484.1"
                     /translation="MDFAIIGGTFNPVHLGHLICAERAYLEFNLDKVIFMPAGNPPHK
                     SEEKIDSAEDRYNMLKLALANNNHFEISEWEIKKQEKSYTADTLEHFKDKYNVERVNL
                     IIGTDSLAEIFNWHQPEYILKNSNLIVAKRNDYSFTKIMKDQRLRKYEDHIHILDNSL
                     IEISSTMIRNFVREGKSIRYLTPDKVIEYINKNNLYR"
     gene            4103..4615
                     /locus_tag="VKN64_04260"
     CDS             4103..4615
                     /locus_tag="VKN64_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMA59485.1"
                     /translation="MIYQFKNNKPKVDDTAFIAPGAKIVGDVELKENTSIWYNAVLRA
                     DLNQMILGKGTNIQENSALHVDTDKPLYIGDYVTVGHSATVHGCKVKNNCLIGMGAVL
                     LNNAVINENSIVAAGSLVTENKEFPAGSLIMGSPAKVVRELTEKEIKSIQESAEHYMK
                     LAQEHKESLK"
     gene            complement(4657..5148)
                     /locus_tag="VKN64_04265"
     CDS             complement(4657..5148)
                     /locus_tag="VKN64_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMA59486.1"
                     /translation="MSNKELEVIKEAILNENEGYQVYSMAAEKINDKEISKSFEKLAN
                     EELKHIDWLKDLYESFEGKRDIEFSIDEISAPDKERLFKMEKIDKENLSFALSVFGIG
                     VKMEKEAIEYYKNAASDTESEEAKNLYKKIIEWEYQHLNFFQEQYDMLREDWWAEQSF
                     EPF"
     gene            5434..5844
                     /locus_tag="VKN64_04270"
     CDS             5434..5844
                     /locus_tag="VKN64_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HMA59487.1"
                     /translation="MEQSLKIAKAALRLAISDRQEENRLKKEYLDNKGIKTAAIDFGG
                     EYNKSVKKIIENAVIAARREDLINETHSNQGAVAGATHEALKQIMGSAVGLNIGGKIG
                     IARREDHLVVAIFFGVGMLNLNEVAVGLSHRTIN"
     gene            6066..7061
                     /locus_tag="VKN64_04275"
     CDS             6066..7061
                     /locus_tag="VKN64_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59488.1"
                     /translation="MVINGKKSLFVVSIFISLTLLFMFSFQMSAQAQDKVFKIGISQF
                     VEHPALDSAREGFIESLSEAGFVEGENIEINLENAQADFSTTQTIAQKFKGQNLDLVL
                     AIATPNAQSAAGVLTDTPLIITAVTDPVAAGLVEDLENKSKPITGTTDMNPVAKQLEL
                     IKQFVPSAEKIGILYNSGEVNSVVQVDIAKEAAADMGLEIVEGTVTNTSEVTLATSSI
                     VGKVDAIYLPTDNTIASAVPSIMKIAKENNVPVFAAEKGQVENGAIATRGIDYFTLGK
                     ETGQIAARVLNGEDPVSIPITGSKNLQLIVNKKSVEEFNLEIPSSLEEEIDILIE"
     gene            7090..7980
                     /locus_tag="VKN64_04280"
     CDS             7090..7980
                     /locus_tag="VKN64_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59489.1"
                     /translation="MDIGFLITSLEQGLIYGIMAIGVYITFRILDFADLTVDGSLTLG
                     AAVSSRLIILGYNPFLTIVFATIAGGIAGSFTGFLNTRLKIAPLLSGILTMTCLWSIN
                     LRIMGRPNIPLLSDNTIFDLFKVPYTVSNYEELILILIVVIAVLITLILFLKTKLGYA
                     VRATGDNPVMVRSMAINTENIKMIGLILANSFVALSGSLVAQYQSFSDISMGIGTIIA
                     GLASVIIGEVIIGKDSLNFIIPGIIIGSILYRASISFALMSGFAASDLKLLTALIVIV
                     FLSAPKIKSIVGGKLNVTTD"
     gene            7964..8764
                     /locus_tag="VKN64_04285"
     CDS             7964..8764
                     /locus_tag="VKN64_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA59490.1"
                     /translation="MLRLINVSKTFNINTCNETKALIDVNLEVKNSDFITIVGGNGAG
                     KSTLLNSIAGVFNLTEGDIILSDKNITDMPEYKRANFIGRVFQDPLMGTSPEMTIEEN
                     LSMALNKNSKLSLKWAINKYKRKKIKEHLSEIDLGLEDRLNTKVKLLSGGQRQALTLL
                     MATIIEPEILLLDEHTAALDPATAIKIKDLTEQIIDNHNITTLMITHDMKDALRFGNR
                     MLMMNRGKIILDVNGKTKENMTINHLLEIFQKKHKEGFYNDRSILSLA"
     gene            complement(8756..10441)
                     /locus_tag="VKN64_04290"
     CDS             complement(8756..10441)
                     /locus_tag="VKN64_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HMA59491.1"
                     /translation="MVKHRWWKESVVYQIYPRSFNDSNNDGIGDIPGIIEKLDYIKKL
                     GIDVIWLCPIYESPNADNGYDISDYRAVMDEFGDLEDLEKLIAEIHKRDMKIIMDLVV
                     NHTSDEHEWFIKSKKSKDNPYRDYYIWKEGKEDNQPPNNWGSFFGGSTWTYDEKTEEY
                     YLHLFTEKQPDLNWENKEVRDNIYEMMRWWLDKGIDGFRMDVINMISKDQSFPDGKKG
                     PNDDYGDGTPYFINGPRLYQYIKEMGENVLFDYDIMTVGETFNFGVENAKKFAKADGK
                     DLLNMVFHFELMSVDRQDDNKWVKKEIDLTEIKDIYKKWNQELYEKGWNAIYLGNHDQ
                     PRIVSRFGDDEEYREESAKMLATMMMTMPGTPYIFQGEEIGMTNVAFDDIGKYRDVET
                     INYMRIQTRKGRSKDKVMEDIHRVSRDNSRTPMQWNDNLNAGFTKSTPWINVNPNYKE
                     INVKNALKDEDSIFYHYQEMIKLRKQNPALIYGRYLPILEEHKQIFAYRRIFEDDEFL
                     VVLNFFGNDVEVSLPAEINLDNYQLVMKNYENKNKKQNSLTLKPYESLVFKLS"
     gene            complement(10468..12783)
                     /locus_tag="VKN64_04295"
     CDS             complement(10468..12783)
                     /locus_tag="VKN64_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HMA59492.1"
                     /translation="MEQLTNNIVSRNRWSIEETKFDSEQIINTGSIFLIGNGYLGYRG
                     TLVEWDSDQYVGCFITDTYDKADDKWKELSNAPNPLYTKVEVGGETLSLFNEETKKNH
                     DIKLDLKHGIYKRETNWQTKEGIEVNVKTARFASLDNIHLIPMQYSITTDKDTNIKFK
                     TGIDGEVWDLNGNHLIDHDTDFQDGLLSVQSYTQEMGIELDVMEGIKFLNDVEYKENI
                     VREDQSVFRVIEFEIKKNETITFYKVGTIYSSNDIKNPYKTAIADCKKAVKSGYEVLK
                     KANKEKWEEIWDKIDIKIEGDPINQTVLRFNLYHNIIATPLHSDELPIGARGLSCQAY
                     QGAAFWDQEIFNLPMFLFTKPDIAKNLLIYRYKTLNGARQKAKYLGYKGAFYAWISGK
                     TGQELCPSYFFKNVLTGRKIHNHFNDWQIHVSPDIAYMVWYYYQVTGDFDFIKNYGAE
                     IVFEVARFLYSHSYYKMDKNRFEFIRLLGPDEYHENVDNNVYTNYISKKALKIALTFY
                     FKLEKENPEVLKHLKEKINLKEKEIIEWEKMEERIYIPTVDRETKLIEQFDGYFDLED
                     VTPDVLEDRLIDEQEYWGWPNGVAVETQVIKQADVIQLFNITNEYSKEVMEKNFKYYE
                     PRTQHGSSLSPSAYSIIASKVGLNKEAFDLFEKSLFIDIGNTNKAISGGTFIGGIHTA
                     ACGAAWQIVVKGFAGMEVLKDGILFKPNIPDKWDSFAFDLTYQNQVIRVKIDKSKIYL
                     NSVTSNKKAVNFTIDKKTYKINPEEYLEIKY"
     gene            complement(12820..13686)
                     /locus_tag="VKN64_04300"
     CDS             complement(12820..13686)
                     /locus_tag="VKN64_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59493.1"
                     /translation="MNKFRENPIRRSLFYLVILIIVIYLLFPFYWALNSSLKSESQLS
                     MTPATFVPRDPQTNEIDFYLTNYEGVFASDKFVRGLFNSLVVATSTTILALTAGSFAA
                     FALGKLRFKGKTPSLYIILAMTMFPQVTVLSGLYAIINALNLGARFSMILTYMLFTLP
                     FTTWVLTAFFRELPIEIMQSAQVDGATPFQTFRLILLPLTAPALVTTGLLSFIAAWNE
                     YLFALTFTTIDPAARTVPVAIQYFSGQFARQEPFGEIMAAAVVVTVPIVVLVLIFQRR
                     IVAGLTAGAVKG"
     gene            complement(13683..14750)
                     /locus_tag="VKN64_04305"
     CDS             complement(13683..14750)
                     /locus_tag="VKN64_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59494.1"
                     /translation="MAKGSSDLSKNEQKLAYRLLIPAFLILIIIAVYPLGQVFYASFT
                     DKEFASSEQTNFIGLENYDKLLSLTIKELPPKINEETGEPIIDEETGKVEYESPIRVL
                     PRKPYLYRPLNEFNIFGNRYVIGSADTDFINSLWNTIIFTVASVFLETLLGLGIALVV
                     NSEFKGRGVMRAVMLVPWAVITVVSARMWEWMFEPSRIGFFNMLFEKFGFSSGNTAFL
                     SMSEYQLPSLIAVDVWKTTPFMALLILAGLQLIPNELYEAAEVDGANKIRQFFTITLP
                     LLKPSLAVALIFRTLDALRVFDVFQVMLAQKRYSMASYNYFQLISARNMGMASAIGVI
                     IFILIFAFAVLYIKMLGVETE"
     gene            complement(14833..16116)
                     /locus_tag="VKN64_04310"
     CDS             complement(14833..16116)
                     /locus_tag="VKN64_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59495.1"
                     /translation="MKRSLTILLVLGLLFTMSFNALAQDDVELTIAGGSVGIELELTK
                     KAARMFEEKHPGVTVNVLSTPDLANDRLGLYLQFFEAESPKVDVYQIDVIWPGDLAQH
                     FLDLYEYGAEDVVDDHFQAIVENNTVDGELVAMPWFTDAGLLYYRTDLLEKYDLEVPE
                     TWEELERAAKTIQTGERAAGNQDFWGYVWQGNAYEGLTCDALEWVYSNGGGTIVNKDK
                     EITINNEKAIEAVNMAADWVGHISPPGVTGLVEESTRKMFEAGNAAFMRNWPYAYSLG
                     NEPEKVISGKFDVAPLPAGDSGNGAATLGGWNLAASKYSNHPEWAAKLALFMAGPEVQ
                     KLRATEGSFNPTIKSLYEDEDVLEANPFFGKLYDVFVNAVARPSTQTAPKYNQTSELF
                     FQSVHSVLTGEEDAETAFAYLELDLQDLLGYEIAE"
     gene            complement(16199..17194)
                     /locus_tag="VKN64_04315"
     CDS             complement(16199..17194)
                     /locus_tag="VKN64_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA59496.1"
                     /translation="MSANMKDVAELAGVSLSTVSRVINDSNLVSKETTLKVKKAINEL
                     DYQINESARALRTNISNLVGIVGAGMDNPFLMKVLKGIEAEAIKENYNIIYGDSDGEL
                     EQELYYINMLKQKKIDGLIIITAKFSDELKEAIRASNIPTVFASGYISEPDIAAVTVN
                     NVKAAEDVSDYLFSLGDYFAIIRGPFRDSVASEERMQGVKNKYNEKEIELKNKYVEEG
                     DFTYESGYSAAETLFKNNDDIKSIFAFSDQMAIGAMRYCFDNGIKIPEDVSIVGFDDI
                     QAARFIRPSLSTVFQSGTELGTESIRILLKMMKGEKVENNKIFVPHKLIKRESSK"
     gene            17430..18728
                     /locus_tag="VKN64_04320"
     CDS             17430..18728
                     /locus_tag="VKN64_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59497.1"
                     /translation="MKGKISTGLTLFLLVSLLLFSLQASAQDGKVTVMGVWGGGELDA
                     FNKMVEPFEAASGIDVQYEGTRDLPTLLTTRLEAGNPPDIVAVSGPGMMKDLAEDGDL
                     VDLTNVFNMNYMEEDFDQKWIDLATYEGGMYAIFIAADAKSLVWYNPNVFEENGYEIP
                     ETWDEMDQLAQQMQEDGITPWSIGLESGAASGWPGTDWIEDIMLRTTSPETYDKWVAH
                     DIPWTDDRVKNAFEIFGKYAKNSDMAYGGSTAVLSTNFGDAPNPLFSDPPRAGLHRQA
                     SFITSFIKENNPELEAGQDFDFFPLPPIDEEYGTPALGAADMLAMINDNQDARQFMQY
                     MTTPGAQSIWVSELGKLGTHNRINPNVYPDDITRQMAAFLNNAETFRFDASDSMPASI
                     GSGAFWQAILNYVSGDNLDSVLQDLENKADEAYNSGEATD"
     gene            18752..19618
                     /locus_tag="VKN64_04325"
     CDS             18752..19618
                     /locus_tag="VKN64_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59498.1"
                     /translation="MQERSKKKWVALFVGPAIFVVGLMLLYPSIHTFVISFFGPDSEA
                     FVGIKNYIYAFTNDMMLVSFRNNLLWLIFMTSFTVGLGLLFAILSDRVKYEKIAKSII
                     FMPMAVSFVGAGVVWKFVYTYRPPGSNQIGLLNQILQSLGMEPIGWLIQGPWINNLAL
                     IFVGIWMWTGFCMVILSASYKNIPQEQIEAAKVDGANEWQVFRFVIFPYMKSTIAVVA
                     TTMVVNVLKIFDIVYVMTNGNFGTEVIANRMYKEMFQYRNFGRASAIAVVLFMLIVPV
                     IFANIKRMRGEG"
     gene            19618..20469
                     /locus_tag="VKN64_04330"
     CDS             19618..20469
                     /locus_tag="VKN64_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59499.1"
                     /translation="MNDNKLAKTIVNVLLITIMLIWILPTIGLLITSVRPASEVSNSG
                     WWTVLNSPLKFTQFTLENYDQVLSVNNMNIAFRNSFIIAIMGTFLPVTIASFAAFGLA
                     KLRFRGRMVIYGIIVGLLVIPLQMTFIPILRIFNFLNISGTFVGLWIAHTGYGMPFAV
                     FMLYNFFSNLPDDLFEAAYIDGASMWTVYYKLALPLSVPALASLIIFQFLWTWNDLLV
                     ALIYLGGTQDVAPVTVRLANMVGSYGQNWHVLTAGAFISIIVPLTLFFSLQRYFIRGI
                     LAGSVKG"
     gene            20485..23553
                     /locus_tag="VKN64_04335"
     CDS             20485..23553
                     /locus_tag="VKN64_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HMA59500.1"
                     /translation="MLPDKIDLKLRDLKKEMFENKYAIKKWNKQEVKRVSESEYEPIE
                     GQKEFLTGDQSYWDNTGKIFFFQREVKLPQKLEKENLYLHLDISGECTLFINGEVYRG
                     INEKNIKLPKTDSNTYHFKILATHDVHLSVRHQRLFNIPYPPHIFRNSFLFNKNKDIE
                     KLYVLLKNIKKTIDVLESKREQLILQNLLEDTLNQIDFFTNKRKEFLTSINKSYQSIV
                     NELQNIENSNVGKVFLLAHSHLDLAFKWTITDSIRKLERTISTTVNLMDSYDNYYFIQ
                     SQSILYEYLKEYYPNLFQKVKEKYRENKFIVEGAFYVESDLNLPHGESLIRQILYGKK
                     FYQDKFDNNTNLAWLPDCFGFSAILPQILKKSEINFFLTTKLQWNETNQFPYNLFKWE
                     GIDGSQISSYLLSDEYGGNLEPKKIYKAWENRKQKDLDEVLSLYGFGDGGGGNNEVQL
                     NNYEALEKIPFLPKIETSDIHNHLNTIFNKNELPTWKDELYLEKHRGTYTSQAELKKY
                     NRKLEFKLRNMELLYSSAKLNGYEKEYNLEKLWKILLKNQFHDILPGSCVHDVYEDAV
                     EDYERLDTGLEDIKNELFAFFSNKYRLDHNQFIIFNPLSFERNDLIEIKKEELEKEYD
                     KEYDSVTINNNKYPVQKSNNSYYFKPNHLSPLSFTKLTFSNNKNIKYENHLNSSQYSI
                     ENKYIKVTLDKKGQISSIFDKEKEIEYIQANQKANQLKLYDDKSTYFDTWDISISEQN
                     KRIIDNIKDIKVKAAGPYFHSIEIKRGFYNSSIRQEMILYENKRKIDFKTEVNWQESQ
                     KLLKTAFPIKIKNSQALFDLSMGHIERANYKNTSWERAKKEVPAHKWVNLNNKDISVS
                     LLNDCKYGHEINENVINLTLLKSGIFPDPKADIGKHEFTYSLLLNPDSLDLVNLEREA
                     LAMNNKPIYCNINNNNEEDNKVKKLFDIKNDHILLDSYKISENKDGFILRVHEFKGIS
                     NQLEIKFLNKIKEVYETNLLEKNVEEISIENNNLTVKINPFEIKTFRIKF"
     gene            complement(23614..23754)
                     /gene="nrdD"
                     /locus_tag="VKN64_04340"
     CDS             complement(23614..23754)
                     /gene="nrdD"
                     /locus_tag="VKN64_04340"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMA59501.1"
                     /translation="MSKKQKCEVYSRVVGYLSPVSEWNDGKQEEYKDRKTYSVNDKLK
                     SS"
     gene            complement(23770..24483)
                     /locus_tag="VKN64_04345"
     CDS             complement(23770..24483)
                     /locus_tag="VKN64_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HMA59502.1"
                     /translation="MNNNIKISGIQKTSLIDYPDYISTVLFTQGCNMDCPYCHNPELI
                     PKENENEYMNTAYFWEYLDKRSDLIDGVVITGGEPLIQDSIYNFIKDIKKYDLLVKLD
                     TNGTNYHLLEKLIDEDLIDYVAMDIKGPLEEYEKFCGIELNKKVIDNIKKSKNLILDS
                     DIDYELRTTVVPGLHSKEVLEKIADSILGVKNYYLQNFRSEKVNSSNYEEKRSFSEDE
                     LKDFVKVFKNKDIKAKIRN"
     gene            complement(24473..>25360)
                     /gene="nrdD"
                     /locus_tag="VKN64_04350"
     CDS             complement(24473..>25360)
                     /gene="nrdD"
                     /locus_tag="VKN64_04350"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HMA59503.1"
                     /translation="CCRLRLDNRELRKRGGGLFGANPMTGSIGVVTLNMPRIGYLAED
                     KEDYMNRVYELMDIAKESLEIKRKVLENLTEQGLYPYSKFYLRNIKKRFDEYWKNHFN
                     TIGINGMNESLENFMGENITEEKAHQFTIEVMEKMKSRMREYQEETENLYNLEATPAE
                     STSYRLARLDKERFGNDIIAANESRVRENDAEPYYTNSTHLPVGYTDDIFDALEIQDD
                     FQIEFTGGTVFHGFLGERLNGIKSTKKLVKRIAANFKLPYYTITPTFSICPVHGYLPG
                     EHKYCPKCEAEERAKASEQ"
BASE COUNT         8114 a         4679 c         3947 g         8620 t
ORIGIN      
        1 ccggtcatcg ggtttgtagc ataactaccc gtaaatatac cttctttttc cgattcaggt
       61 gaggttcttt ccatctcttt ttgtttctgt acctttttaa taaaatctcg aattttatct
      121 tcattttctt taccggaaat caattcatct acataaggat gttccggggc taaaaccata
      181 taggtggctc cataaattgt atcagggcga gtagtaaaga cctcaagctc ttccccaatt
      241 tcctcgatgg ggaagttaat actcatcccc tcacttctac cgatccagtt tttctgcata
      301 gttttaactc tttccggcca gtctaattta tcatggcctt ccagcagttc atccgcatat
      361 tcagtaattt ttaaaaacca ctgttctaat tctttttgtt ctacctctgt tccacatctt
      421 tcacagctgt tattgactac ctgttcattt gccaagaccg tatcacagct gggacaccag
      481 ttaacagcct gttcttttct ataggctaaa tcattctcaa acatctttac aaaaaaccac
      541 tgggtccatt tatagtaatc agggtcagca gtcgcaactt cccggtccca atcataactg
      601 aggcccattg ctctcatctg ttctttcata ctttcaatat tttcccaggt ccattccctc
      661 gggtgaatat taccatgttt aatagctgca ttttcagccg gtaggccaaa ggcatcccaa
      721 cccatagggt gaagtacatt atatcctttc atgcgcttaa aacgggctat tacatcccct
      781 atagaataaa cccttacatg acccatatgt aatttgcctg agggataggg aaacatctct
      841 aatacatagt agttatcttt ttcatcttta tttttagttt tatacaaaga tttttcctgc
      901 caatctttct gccattttcc ttcaatcggt tcaaaattat agtgactttc cattacaatc
      961 tcctccctga aatatatgat tattatttaa aaataaaaaa actcctcccg gtcaagggac
     1021 gagttagtta cccgtggtac caccctaata gatagtaatt atccactcaa aaaagttaac
     1081 gcctttttta cgatactgct tacttgcttc aacagtatag gttccggaac gagttcgact
     1141 tttttcatta ctgcttttca gctgccagca gctctctttt aaatgaaaag aagtctaatg
     1201 ctttccttca taaccttatt tatcattgtt tatctttgta aaaattatat taatttatta
     1261 acttaaagtc aagaagtttc ttcttttttt aatattttct tggcatcagc ccatagcctg
     1321 tccagttcat agtattctcg ttctctttta tggaaaatat ggacaattac atcggcataa
     1381 tccattaata tccagcgtcc gttgtcttta ccggcaattt gtttgggata taccccttct
     1441 tcatccagtt tttcttccac agcacgtgca atagcctgga cttgtttatc cgtattaccg
     1501 gaacataaaa caaagtaatc agctatgata gttagtcctt ctacatttaa gatctctata
     1561 tcgaaagctt ttttatcgtc agctgcttct gctgctaact ctgctattct cttataattt
     1621 tcttctggca ttaattaatt tcctcctttt tttgctattt gattttctac aaagttctgt
     1681 cccagaatta tctggaagtc tacttctatt tcttcattct tttctatttt gccgcccatt
     1741 acatccttta gtcctcgggc taccttttta ttaccattct ggtaatatat aatggtttcc
     1801 tgataattga agtcctttgc attagctact tccttaatat taaagccaaa tttttctaac
     1861 tcttcggctg cttcaccggc taccccagaa acaccgttac cattgagaag cgttatctgg
     1921 tattggtcgt tttgaatata ttctttactt ctaattagat tattaaccat aatctttaaa
     1981 tcttcttccg taggtatcca gtaactagcc ctattaatat actcaggtcg gcctggcacc
     2041 atctcggttt ttaaattatt taagttagtt tttttggcta acttagcaaa gggagttaca
     2101 tcttttaagg gaatattagt attaacagaa tccattactt ctttataaat cgagggtaaa
     2161 ttaaggagaa cttttgatga tacagctttt tccataactg cctgtataaa cttttgctgt
     2221 ctggcaaccc tgcctatatc tcctctggta cccctatatc taacgtattc taaagccttt
     2281 tcaccattta gatggacatt tccggccggt aaatcaatat ataagccgcc ggctttatca
     2341 acataatgga gaggcttttc tattgttata tcaattcccc caattaagtt tataatctct
     2401 gcaaaacctt gaaaatctgt ttcaacatag taatccagct ttatatttaa aaaatcttct
     2461 acagtttctt tcagtaagtc aataccccca taagcatgtg aggcattaat cctatcataa
     2521 ccatggccat ttaccttcac cctggtatca cgcgggataa atagtaggcc gatattgtta
     2581 gtatctagat tgacactggc cacgataatt gtatccgccc ggggtggacc attaattgaa
     2641 gaatcataac cggctatcaa aatattcatt ttagattttc ggaaagggtt taagtttact
     2701 aaactaaact catcatttaa taatatatat agagataaac ttataattac aattaaaagg
     2761 aaaataccaa caattttcca gtttatattt tttaatcttt ctaacattta tttggctccc
     2821 ttcaagagtg agttccttgt ataaagtgta tttggatgaa ttactcgttt tcggtcaata
     2881 ttatatttaa ttgtaaaatc acagactaat atgactgctt catctaaatc tttatctatc
     2941 ttctctctta ttaaatcaac tccagggaaa ttcctgcctg gttctattac atctgctaca
     3001 aatatgattt tttctaacct gctcatcccc gttctgccaa tcgtgtgaaa ccttattgcg
     3061 tttaaaatat cttcatcttt aatcccaaat ttatgttctg ctaaataagc acccaccgga
     3121 gaatgaagta aagcggggat ctctttttct aagtcatcaa cttcaatatt attttcttca
     3181 accattttta gcagtttttc gtcctcataa tgacgggcaa aatcatgtaa tagagaagct
     3241 attttaactt tatctatctc aactggatat ctctttgcta tttttaaagc tgtttcttct
     3301 acagcctgag tatgttttat ccgaaagttt ttagttttct ttaatttttc ttttaatttt
     3361 tttatttcta ccatttatgt cacctataga ggttattttt attgatatac tcaatcactt
     3421 tatcaggtgt gagatatcta attgacttcc cttcccttac aaaattcctt atcattgtag
     3481 aggaaatttc aatcaaagaa ttatccaaaa tatgtatgtg atcttcgtac tttcttaatc
     3541 tctgatcttt cattatcttc gtaaaagaat agtcattacg ctttgcaact attaaattag
     3601 aatttttaag aatatattca ggttgatgcc agttgaatat ttccgccaaa gaatcggtcc
     3661 caattattaa attaaccctc tcaacattat atttatcttt gaaatgctct aatgtatcag
     3721 ctgtatagga tttttcctgt tttttgattt cccattcaga aatttcaaag tggttgttat
     3781 tggccagggc caattttaac atattatacc tgtcttcagc tgaatctatc ttttcttctg
     3841 atttatgggg aggattaccg gcaggcataa aaataacctt atccagatta aattccagat
     3901 aggctctctc tgcacatatt aaatgtccga ggtgaaccgg attaaatgtt cctccaataa
     3961 ttgcaaaatc cataatctac tccttccatt ttacttattt aattataata aataaattta
     4021 gtatattcaa gtaaattaat taactaagaa ggattaactt tacttgtgtt gaaataatga
     4081 aaaatataag gaggtaatag agatgatcta tcaatttaaa aataataaac caaaagtaga
     4141 cgatacagct tttatcgcac ccggtgcaaa aattgtagga gatgttgagc taaaagaaaa
     4201 tacgagcatc tggtataatg ccgttttaag agctgattta aaccaaatga ttttgggaaa
     4261 aggtacaaat atacaggaaa attcagcttt acatgttgat actgataaac ctttatatat
     4321 aggtgattat gtgacagtcg gtcacagcgc cacagtacac ggctgtaaag ttaaaaataa
     4381 ctgtctaatt ggcatggggg ccgttttatt aaataatgct gtaatcaatg aaaattcaat
     4441 tgtagcagcc ggcagccttg taacagaaaa taaagaattc ccggctggaa gcttaattat
     4501 gggttctccc gcaaaggtag taagagaatt aactgaaaaa gaaattaaaa gtattcaaga
     4561 atcagccgaa cactatatga aattagcgca agaacataaa gaaagtttaa aataaactaa
     4621 agccctgctt aataaaaagc agggctttca tcttctttag aagggttcaa aactttgttc
     4681 agcccaccag tcttctctca gcatatcata ctgttcctgg aagaagttta aatgttgata
     4741 ctcccattca attatctttt tataaagatt tttggcttct tctgattcag tatcactggc
     4801 agcgttttta taatattcaa ttgcttcttt ttccatctta acaccaattc caaagactga
     4861 aagagcaaat gacaaatttt ctttatctat cttttccatt ttaaataatc tttctttatc
     4921 cggggctgat atttcatcaa tagaaaattc aatatcccgc tttccttcaa aactttcata
     4981 aagatctttt aaccaatcga tgtgctttaa ttcctcattg gctaattttt caaaggattt
     5041 acttatttcc ttgtcattta ttttttccgc cgccatggaa taaacctgat aaccttcatt
     5101 ttcatttaaa atagcttctt tgattacctc taattcttta ttactcatta ataatccccc
     5161 tttataattt ccttataaaa cctccctgca gcctacttgg tgtttttctt tttacaatga
     5221 tcaaaataat tattcaattt ttatatttac ctttaaaatc tttagttaag agcaaggatc
     5281 tgacactaat aaatttttgt tacttaactt gtttatctta ggtaaaatat atcagataaa
     5341 gggggtattt tcaagtaaat taaattacat ccaattgaca taaatttata aataaaatat
     5401 aattaaagta aatataagta ggaggcttac aaaatggaac aaagccttaa gattgccaaa
     5461 gcagctttaa gattagctat ttcagaccgg caggaagaaa acagacttaa aaaagagtat
     5521 cttgataata agggtataaa aactgcagca attgattttg gtggtgaata caacaaatct
     5581 gttaaaaaaa ttattgaaaa tgcagttatt gctgcccgcc gcgaagattt aatcaatgaa
     5641 actcattcca accagggagc agtagcagga gcaacccatg aagctttaaa acaaatcatg
     5701 ggaagtgcag tcgggttaaa tatcggaggg aaaattggta ttgcccgcag ggaagatcac
     5761 ctggtagtag ccatcttttt tggagttggc atgcttaact taaacgaagt tgctgttggt
     5821 ttaagtcaca gaactattaa ttagctataa ttctacttta atttcttgac aattgctcaa
     5881 ataatgtgta taatgaaaaa aatcaaaaac tggtaatatg gtagtatggt aggagaaata
     5941 ataggaaatt tctatgtatt tctatgtctt taaacactcc ttccaggagt gtttttttat
     6001 ttattttctc aaatttctct ttccaaaaaa ccaaacacca aaaacaaaat aaggagagat
     6061 gcaaaatggt aattaatggt aagaaatctc tatttgtagt atctattttc atttcattaa
     6121 ctctactttt catgttttca tttcaaatgt cggctcaagc tcaggataag gtatttaaaa
     6181 taggtatatc tcaatttgtt gaacaccctg ctttagattc agctcgggaa ggttttatcg
     6241 aaagtttaag tgaggctgga tttgtagagg gagaaaatat tgagattaat cttgaaaatg
     6301 cccaggccga tttctcaaca actcaaacca tagctcaaaa attcaaagga caaaatcttg
     6361 atcttgtctt agcaatagca actcctaatg cacaatccgc agcaggggtt ttaaccgata
     6421 ctcctctaat tattactgct gttacagatc ctgtggcggc cggactggtt gaagatttag
     6481 aaaataaaag taaaccaata accggaacaa ctgatatgaa tcccgttgct aaacagctgg
     6541 aattaattaa acaatttgta ccatccgcag aaaagatcgg tattttatat aatagtggtg
     6601 aagtcaactc agttgtacag gtagatatag cgaaagaagc tgcagctgat atgggcctcg
     6661 aaattgtaga aggtacagta actaatacca gtgaagtgac cttagcgacc tcttctattg
     6721 tcggtaaggt agatgccatc tacttaccca cagataacac aattgcatca gctgtgccct
     6781 caattatgaa aatagctaaa gaaaataatg tccctgtctt tgcagctgaa aaaggtcagg
     6841 tagaaaatgg agcaatcgca actcgaggta tagattattt cacattaggt aaggaaacag
     6901 gtcaaatcgc agccagagta ctaaatggag aagatccagt ctcaatcccg attaccggtt
     6961 ctaaaaacct tcagctaata gtaaataaaa agtcagttga agagttcaac ttagaaatac
     7021 caagtagcct agaagaagaa atagatatac taattgaata atataggata agacattgga
     7081 ggttttaata tggacatcgg ctttttaata acttctttag aacagggttt aatctatggg
     7141 attatggcta ttggagttta cattactttc agaatactgg actttgcaga cctaactgta
     7201 gacggcagcc tcactttagg ggctgctgtc tcctcccgac ttatcatttt gggatataat
     7261 ccattcttaa caattgtatt tgctacaatt gcaggaggta ttgcaggttc atttacaggg
     7321 tttttaaata cgagacttaa aatcgctccc ttgttgtcag gaatcttaac aatgacctgt
     7381 ctctggtcaa taaatctaag gataatggga cggcccaata ttcctctttt atctgataat
     7441 acaatttttg atttgtttaa agttccctat acagtctcaa attatgaaga attaatttta
     7501 atattaattg ttgtaatagc agtattgatt acccttatct tatttttaaa aacaaaattg
     7561 ggttatgctg ttagagcaac tggagacaat cccgtgatgg taagaagtat ggcaatcaac
     7621 actgaaaata tcaaaatgat cggccttatt ttagctaata gttttgtggc tctttcaggt
     7681 tctttagtag ctcaatatca atccttttcc gatataagca tggggatagg tacaattata
     7741 gcaggactag cctcagtcat aatcggggaa gtgattatcg gtaaggacag tttaaatttc
     7801 attattccgg gtattatcat cggttctatc ttatatagag ccagtatttc attcgcattg
     7861 atgagcggtt ttgctgcttc tgatcttaaa ttattaaccg ctttaattgt tatagtattt
     7921 ctttcagcac caaaaattaa gagtatagtg ggaggtaagt taaatgttac gactgattaa
     7981 tgtaagtaaa acttttaata taaatacatg taatgaaaca aaggctttaa tagatgtaaa
     8041 tttagaagtc aaaaatagtg atttcattac aatcgtggga ggcaatgggg caggtaagtc
     8101 taccctttta aacagtatcg ccggggtatt caacttaacg gaaggtgata ttatactgtc
     8161 cgataaaaat attaccgata tgccggaata caaacgggct aattttatcg gccgggtctt
     8221 tcaggacccc ttgatgggta cttcacctga gatgactata gaagaaaatt tatcaatggc
     8281 ccttaataaa aattctaaac tttctttaaa atgggccatt aataaatata aaagaaagaa
     8341 gattaaagaa catctatctg aaattgacct cggtctcgag gatagactaa acactaaggt
     8401 aaaactactt tcaggcggcc aaagacaggc tttaacccta ttaatggcta caattataga
     8461 acctgaaatc ttgcttttag atgaacatac tgcagcatta gatccggcta cagccatcaa
     8521 gattaaagac ctaaccgaac aaattattga caatcataat atcacaactt taatgattac
     8581 acacgatatg aaagatgcct taagatttgg aaatagaatg ctcatgatga accgcggtaa
     8641 aattatcctt gatgttaacg gtaagaccaa agaaaatatg accatcaacc atctattaga
     8701 aatcttccag aaaaaacata aagaaggctt ctataatgat aggtctatct taagtttagc
     8761 ttaatttaaa aacaagactc tcatatggtt ttaaagtcag agagttttgt ttcttatttt
     8821 tgttctcata atttttcata actagctgat agttatctaa atttatttct gctggtaaag
     8881 atacctcaac atcattacca aagaagttta aaacaacaag aaactcatca tcttcaaata
     8941 tacgtctata agcaaaaatc tgtttatgtt cttctaatat gggtaggtat ctgccgtata
     9001 ttaaagcagg gttttgcttt cttaatttta tcatttcttg ataatggtaa aatattgaat
     9061 cctcatcttt tagagcattt ttaacgttta tctccttata atttggatta acatttatcc
     9121 agggggtact ttttgtaaag ccagcgttta gattatcatt ccactgcata ggggttctag
     9181 aattatctct gcttacccta tgtatgtctt ccatcacttt atctttagat ctccctttcc
     9241 tggtttgaat cctcatataa ttgatggttt caacatctct gtactttcca atatcatcaa
     9301 aagctacatt tgtcatcccg atttcctctc cctgaaaaat gtagggggta ccgggcattg
     9361 tcatcatcat ggtggccagc attttggccg attcttctct atactcctca tcatcaccaa
     9421 atcgagatac aattctcggt tgatcatggt tgccaagata tattgcattc caaccttttt
     9481 catataattc ttgattccat tttttatata tgtcctttat ttcggtaagg tctatttctt
     9541 ttttaaccca tttattatca tcctgacggt ctacggacat caattcaaag tgaaaaacca
     9601 tgttaaggag atcttttcca tccgctttag caaacttctt ggcgttttct accccaaaat
     9661 taaaggtttc tcccacggtc attatatcat aatcaaatag tacattttct cccatctctt
     9721 taatgtattg ataaagtctt ggaccattaa taaaataggg agtaccgtcc ccataatcat
     9781 cattggggcc ttttttacca tcagggaaac tttggtcttt agagatcata ttgattacat
     9841 ccatcctaaa accgtctatg cctttatcta accaccatct catcatctca tatatattat
     9901 ctcttacttc tttattttcc cagtttaaat caggttgttt ttctgtaaat agatgtagat
     9961 agtattcttc tgttttttca tcataggtcc aggttgaacc accaaaaaat gacccccagt
    10021 tattaggagg ttggttatct tctttgccct ctttccaaat ataataatcc cggtatggat
    10081 tatccttaga ttttttagat ttgataaacc attcatgttc atctgatgta tgattaacca
    10141 ctaaatccat gattatcttc atatctcttt tatgaatttc agcaatcaat ttttctaagt
    10201 cctctaaatc accgaattca tccattactg ccctgtaatc actgatatca tagccattat
    10261 cggcattagg tgactcgtat atagggcaga gccaaattac atcaattcct aactttttaa
    10321 tataatctag tttttctatt atgccgggga tatctcctat tccatcatta ttgctgtcat
    10381 taaaactgcg cggatatatt tgatagacaa ccgattcttt ccaccatctg tgtttaacca
    10441 ttatattttc cctgcctttc aaacttgtta gtatttgatt tctaaatatt cttcaggatt
    10501 tattttatag gtttttttgt cgattgtaaa attaactgct tttttatttg atgtaacaga
    10561 atttaaatat atcttgcttt tatctatttt aacccttatt acctgatttt gataagttaa
    10621 atcaaaggca aagctatccc atttatctgg gatatttggt ttaaacaata taccgtcctt
    10681 taatacttcc atcccggcaa aaccttttac aactatttgc caggcagcac cacaggcggc
    10741 ggtatggatt cccccaataa aggttcctcc actgattgcc ttatttgtat taccaatatc
    10801 aataaacaaa gatttttcaa ataagtcaaa ggcttcttta ttcaatccca ctttggaagc
    10861 aataattgaa taagcagaag gacttaaaga ggaaccatgc tgtgttcttg gttcataata
    10921 cttgaagttt ttttccatta cttctttact atactcattt gtgatattaa aaagctgaat
    10981 gacatccgcc tgttttatta cctgagtttc aacggctacc ccattgggcc agccccagta
    11041 ttcctgttca tcaattagtc tgtcttctag cacatcaggg gtaacatctt ctaaatcaaa
    11101 gtaaccatca aactgttcaa tcaatttggt ttccctatct actgtaggga tatatattct
    11161 ctcttccatc ttttcccatt ctattatttc tttttctttt aaatttatct tttcttttag
    11221 atgcttaagg acttcagggt tttctttttc taatttgaaa taaaaagtaa gagcaatttt
    11281 taatgccttt ttacttatat agttagtata tacattgtta tccacgtttt catggtattc
    11341 atccggccct aacagcctga taaattcaaa tctattttta tccatcttat aataactatg
    11401 agaatacaag aagcgggcta cttcaaaaac tatttcagcc ccataatttt taatgaagtc
    11461 aaaatcacct gtaacttgat agtaatacca aaccatataa gctatatcgg gggaaacatg
    11521 aatctgccag tcgttaaaat gattgtggat cttacggcct gtcaatacat ttttgaaaaa
    11581 ataggaagga cagagttctt gcccggtctt accacttatc caggcataaa aggccccttt
    11641 ataaccaaga tatttagcct tttggcgggc gccatttaga gttttatatc gataaattaa
    11701 caagtttttg gcaatatcag gtttggtaaa taaaaacatc ggcagattaa agatttcctg
    11761 gtcccaaaaa gcagctccct gataggcttg acaggataaa ccgcgggctc caatgggaag
    11821 ttcatcagaa tgtaggggtg ttgctataat attatggtat aaattaaacc ttaaaactgt
    11881 ctgatttatg ggatcacctt caatttttat atctatttta tcccaaattt cttcccattt
    11941 ttccttgttt gctttcttga ggacttcata gcccgacttt acagcctttt tgcaatcagc
    12001 tattgcagtt ttataaggat tttttatatc attactgcta tagatcgttc ctaccttata
    12061 aaaagtaata gtttcattct ttttaatttc aaactctata actctaaaga cagattgatc
    12121 ttctcgtaca atattttctt tatattcaac atcatttaaa aacttaatcc cttccattac
    12181 atcaagttca attcccattt cttgagtata tgactgcaca gataacaaac catcttggaa
    12241 atctgtatca tggtctatta aatgattacc gtttagatcc caaacctcac catcaatacc
    12301 tgtcttgaat ttaatgttag tatctttatc agtagtaata gaatactgca tcgggataag
    12361 atgtatatta tccaggctag caaatctggc tgtttttaca ttaacttcta taccctcttt
    12421 tgtctgccag ttggtctccc tcttatatat cccatgttta agatctaatt taatatcatg
    12481 gttctttttg gtctcttcat taaaaagaga aagagtttct cccccaacct caactttggt
    12541 atataaagga ttgggggcat tagacaactc tttccatttg tcatcagctt tatcataggt
    12601 gtcagtaata aaacaaccta catattgatc agagtcccat tctactaagg ttcctctata
    12661 ccccaaatag ccgttaccaa ttaggaaaat actgcccgta tttataattt gttcgctatc
    12721 aaatttagtt tcttcgattg accatctatt tctactaact atattattag ttaattgttc
    12781 catactaaat tttcctcctt gattgtttgg ttatattatt tatcctttta cagctccggc
    12841 agttagtcca gctactattc ttcgctggaa gatcaatact aggaccacaa tgggtactgt
    12901 aaccactaca gcagctgcca tgatttcccc aaatggttcc tgccgcgcaa actgaccgga
    12961 aaagtattga atagctaccg gaacggttct agctgctgga tcaattgtgg taaatgttaa
    13021 ggcaaatagg tattcattcc aggctgcaat aaatgataaa agacctgtag ttactaaagc
    13081 tggagcagtt aagggcaata gtatcaacct aaaggtttga aaaggtgtag caccatcgac
    13141 ctgagctgac tgcatgatct cgatcggtaa ttctctaaag aaagcagtta aaacccaggt
    13201 ggtaaagggt aaagtgaata acatataagt taagatcata ctgaatctag ccccaaggtt
    13261 taaagcattt atgatagcat ataagcctga taaaacagtt acctgtggga acattgtcat
    13321 agccaggatt atatataagg aaggggtttt ccctttaaat cttaatttac ctaaggcaaa
    13381 agctgcaaag gaaccagctg taagtgctaa aatagtagta ctggtagcaa caactaaact
    13441 gttaaataac cccctcacga atttatcact ggcaaataca ccttcatagt ttgttaaata
    13501 aaaatcaatt tcattggtct gaggatcacg cggaacaaag gtagcgggcg tcattgataa
    13561 ttgactttca gattttaagg aagaatttaa agcccagtaa aagggaaata ataaataaat
    13621 tactataatt aagattacta aatagaacaa agaccttctg atgggatttt ctctaaattt
    13681 attcattcgg tctcaactcc taacattttg atgtacaaga ctgcaaatgc gaatatcaaa
    13741 ataaatataa taacaccaat tgctgaggcc attcccatat tacgagcact tattagttga
    13801 aagtagttat aacttgccat tgaatatctt ttttgtgcca gcataacttg gaaaacatca
    13861 aatactctta aagcatctaa tgttctaaat attaaggcga cagctaagga aggctttaat
    13921 aaaggtaatg ttatagtaaa gaactgtcta attttattag caccatctac ctcagccgct
    13981 tcatatagtt cattgggtat cagctgaagc cctgctaaaa ttaaaagcgc cataaaaggt
    14041 gttgttttcc atacatctac agcaatcaat gagggtaatt gatattctga cattgataaa
    14101 aaggcagtat taccagatga aaagccaaat ttctcaaata acatattaaa aaatcctatc
    14161 ctactaggct caaacatcca ttcccacatc cgagccgata caactgtgat gacagcccag
    14221 ggaactaaca taaccgctct cattactccc cgacctttaa actcgctgtt tactactaaa
    14281 gctataccta aacctaatag tgtttctaag aagactgaag caaccgtgaa gataatagta
    14341 ttccaaagtg aattgataaa atctgtatcg gcagatccta ttacatatct attaccaaat
    14401 atattaaatt catttaatgg tctatataaa taaggtttgc gcggtaatac tctgatagga
    14461 ctttcatatt caacttttcc cgtttcttca tcaataatag gctcccctgt ttcctcattt
    14521 attttagggg gcaattcttt aattgttaaa cttaacaatt tatcataatt ttctaaacca
    14581 ataaaattgg tttgttcaga actggcaaat tccttatcag taaaactggc ataaaagacc
    14641 tgacctaagg gataaacagc tatgataatc aagatgagaa aagctggtat tagtagtcta
    14701 taagcaagtt tttgttcatt tttggacagg tcactggaac cttttgccat gatattttac
    14761 ctcctcttaa tttaagaatc gaggacctcc ccttaggagg tcctctttaa tattatccaa
    14821 ttgattaact ttttattcag caatttcata tcctaagaga tcttgcaagt ccaattctaa
    14881 ataagcaaaa gcagtttcag catcttcttc accagttaat acactgtgca ctgactggaa
    14941 gaagagttca gatgtttggt tatattttgg agcagtttga gtagaaggac gtgctaccgc
    15001 attgacaaaa acatcatata gcttaccaaa gaatggatta gcttctaaaa catcttcatc
    15061 ttcatatagg gatttaatag ttgggttaaa ggaaccttca gttgcacgta atttttgcac
    15121 ttctggtcca gccataaata gagctaactt ggcagcccat tcaggatggt tactatattt
    15181 actggctgct aagttccagc cacctaaggt agcagcacca ttgccgctat ctccagccgg
    15241 tagaggtgca acatcaaatt taccggaaat tactttttcg ggttcattac ctaaagaata
    15301 agcatatggc cagttacgca taaacgcagc attgcctgct tcaaacattt tacgtgtact
    15361 ttcttcaact aaaccggtaa ctccaggagg agaaatatgt cctacccagt cggcagccat
    15421 attgacagct tcaattgctt tttcattatt tattgtaatc tctttgtctt tatttacaat
    15481 tgtgccgccg ccattagaat agacccattc taaagcatca caggttaaac cttcataagc
    15541 attaccctgc catacataac cccagaagtc ctggttacca gcagctcttt caccggtttg
    15601 aatggtttta gctgctcttt ctagttcttc ccaggtttct ggaacttcta gatcatattt
    15661 ttctaataga tctgttctat aatagagtaa gcctgcatct gtaaaccagg gcatagcaac
    15721 caattcaccg tcaacagtat tattttcaac tatggcttgg aagtggtcgt ctacaacatc
    15781 ttcagcacca tattcatata agtcaaggaa atgctgagct aggtcaccag gccagataac
    15841 atcgatctga taaacatcaa cttttgggct ttctgcttca aagaactgta gatataaacc
    15901 taaacggtca ttggcaaggt caggtgtact taatacatta acggtaacac ctggatgttt
    15961 ttcttcaaac attctagctg ccttttttgt taactccagt tcaataccca cagagccacc
    16021 cgcaattgtc aattctacat catcctgcgc taaagcattg aaactcattg tgaatagtag
    16081 accaagtact aacagaatgg ttaaacttct tttcattaaa ttattcctcc ttttaaaatt
    16141 ttatagtatc ttgtttttta aaatgtgatt tcttttcaaa atcacctcct aaaacttatt
    16201 atttactgct ctctcttttt attaatttat gggggacaaa aattttatta ttttctactt
    16261 tttcaccttt catcattttt aataatattc taatactttc ggtacctaat tcagttccgg
    16321 attgaaaaac agtacttaga gaaggtctaa taaaacgtgc agcttgaata tcatcaaacc
    16381 caacgataga tacatcttca gggattttga tcccattgtc aaaacagtat ctcatcgcac
    16441 caatcgccat ctgatcactg aaagcaaaga tagattttat atcatcattg tttttgaaaa
    16501 gggtctcagc tgcagaatag ccgctttcat aggtgaaatc accttcttca acatacttat
    16561 tttttagttc aatttctttt tcattatatt tattttttac tccctgcatt ctttcttcac
    16621 tagctactga atctctaaat ggacccctaa taattgcaaa ataatcccca agactaaata
    16681 aataatcact gacatcttca gctgccttaa cattattaac tgttactgct gctatatcag
    16741 gttctgaaat atagccggag gcaaatacgg ttggtatatt actggcccgg attgcttctt
    16801 ttagctcatc ggaaaatttg gctgtaataa tgataagtcc atcaatcttt ttttgtttta
    16861 acatattaat atagtatagt tcctgttcta actcaccgtc agaatcacca tagataatat
    16921 tatagttctc cttaatagct tctgcctcaa tacctttgag tactttcatc agaaaaggat
    16981 tatccatgcc tgctccaaca atacccacca aattacttat atttgttctt aaggctcgcg
    17041 ccgattcatt aatttgataa tctaattcat ttattgcttt tttaactttt aaagttgtct
    17101 ctttactaac tagattactg tcattgataa ctctggacac cgttgataaa gaaaccccag
    17161 ccagttctgc cacatccttc atatttgcac tcataattac tcccctcatt tttgaaaacg
    17221 atttctgaaa accatttcat taattattta tttcaatgta aaatttaata ttcctgctta
    17281 aaatttaaat tatttttaat tttttataat tttcatacaa ttatccagcc tgatagtgaa
    17341 tttattcagt ttgcagggtt tttttaattt ttcaagaata taagatatac gatggaacaa
    17401 aattaaagaa agaaaggggt tttaaaacaa tgaaaggaaa aatttccact ggtttgacat
    17461 tgtttttact ggtaagtctc ttactcttct ctcttcaggc ttctgcacaa gatggtaaag
    17521 ttaccgttat gggagtctgg ggtggtggtg aactcgatgc atttaataaa atggtcgaac
    17581 cattcgaagc agcctcaggc attgatgtgc agtatgaagg tacaagagat cttcctacac
    17641 tccttacaac aagactcgaa gcaggtaatc cacccgatat tgttgctgtc tccggtccag
    17701 gtatgatgaa agatctggct gaagatggtg acctagtcga tcttacaaat gtatttaaca
    17761 tgaattatat ggaagaagat ttcgaccaaa aatggattga cctagcaact tatgaaggcg
    17821 gtatgtatgc aatctttatt gctgcagacg ctaaaagctt agtctggtac aatccaaatg
    17881 tctttgaaga aaacggctat gaaattcctg aaacctggga tgaaatggat caattagctc
    17941 aacagatgca ggaagatgga attactccct ggtctatcgg attagaatca ggcgctgcta
    18001 gtggttggcc cggcacagac tggattgaag atattatgtt aagaaccact tcacccgaaa
    18061 cttatgataa atgggtagct catgatattc cctggaccga tgatcgtgtc aaaaacgcat
    18121 ttgaaatatt cggtaaatat gctaaaaact cagatatggc ttacggcgga tctactgcag
    18181 ttttatcaac taactttggc gatgcaccca atccactatt cagtgaccca ccaagagccg
    18241 gactacaccg tcaggcaagc tttatcacca gcttcattaa agaaaacaat cctgaattgg
    18301 aagcaggaca agacttcgat ttcttcccac tacctccaat tgatgaagaa tatggaacac
    18361 ctgccttagg tgctgcagat atgttagcaa tgattaatga taatcaggat gcaagacagt
    18421 tcatgcagta tatgaccact ccaggcgctc agtcaatttg ggtaagtgaa ctaggtaagt
    18481 taggtaccca taatagaatt aatcctaatg tatatcctga tgatatcaca agacagatgg
    18541 cagccttctt aaataacgct gaaacattta gatttgatgc ttctgactcc atgccagctt
    18601 caattggttc tggggcattc tggcaagcta ttctcaacta tgtaagtgga gataatctgg
    18661 acagtgtatt gcaagatcta gaaaataaag ctgatgaagc atataattca ggtgaagcaa
    18721 cagactaaag ttaagccggg aggtttaata tatgcaggaa agaagtaaga aaaaatgggt
    18781 ggccctcttt gtagggcctg ccatttttgt tgttggactg atgttactct atccctctat
    18841 tcatactttt gtaatcagtt ttttcggacc agattcagaa gcatttgtcg gtattaaaaa
    18901 ttatatttat gcctttacca atgatatgat gcttgtctct tttagaaata atctcttatg
    18961 gttaatattt atgacatcat ttaccgttgg tttaggcctt ttgtttgcta ttttatccga
    19021 tagagttaaa tacgaaaaga tagcaaaatc tataatcttt atgccgatgg ctgtttcctt
    19081 tgttggcgct ggtgtagttt ggaagtttgt ttacacttac cggcctccag gttctaacca
    19141 aatcggtcta ctcaatcaaa tattgcaatc tctcgggatg gagccgatcg gttggctaat
    19201 acagggaccc tggattaata atttggcctt gatatttgtc ggtatctgga tgtggaccgg
    19261 attctgtatg gttattttat ctgcatccta taaaaatata ccgcaggaac aaatagaggc
    19321 cgctaaagtt gatggtgcaa atgaatggca ggtctttagg tttgttatct tcccctatat
    19381 gaaatctacc attgctgttg tagcaactac catggtagtt aatgtactga aaatatttga
    19441 tattgtttat gttatgacaa atggtaattt tggtaccgag gttattgcga acagaatgta
    19501 caaagaaatg ttccagtaca gaaacttcgg tagagccagt gcaatagctg ttgttctctt
    19561 catgttaata gtgccagtaa tctttgcaaa tattaagaga atgcgggggg aaggataatg
    19621 aatgataata agttagcaaa aacaattgtt aatgttttat taataactat catgttgatc
    19681 tggatcttac ctactatcgg attattgatt acttctgtaa gacccgcttc tgaggtaagt
    19741 aatagtgggt ggtggaccgt tcttaacagc cctctcaaat ttactcagtt tacccttgaa
    19801 aactatgacc aagtcttgtc tgttaataat atgaatatag catttagaaa cagtttcatt
    19861 attgctataa tgggaacatt tttaccagta actattgcat cctttgctgc ttttggactg
    19921 gctaaattaa ggttcagagg gcgaatggtt atctatggta tcatagtagg attactagta
    19981 ataccacttc aaatgacctt tataccaatt cttagaatct ttaatttctt aaatatatca
    20041 ggtacttttg taggtttatg gatagctcat accggctatg gaatgccctt tgcggttttt
    20101 atgttatata atttcttttc caacctgccg gatgatctat tcgaggcagc ttatatagat
    20161 ggggcttcaa tgtggacggt ctattataaa ttggctttac cgctgtccgt acctgcctta
    20221 gcctcactaa tcatattcca atttctctgg acctggaacg acttattggt tgccttgatt
    20281 tatttaggcg gtactcagga tgttgctcca gttacagtaa gattggctaa tatggttggt
    20341 tcttacggtc aaaactggca tgtactaaca gccggtgcct ttatttcaat catagtaccc
    20401 ttaacactat tcttctcact acaacgttac ttcattagag gtatattagc aggatccgtg
    20461 aaaggataaa aaggagaaat aaatatgtta ccagacaaaa tagatttaaa attaagagat
    20521 ctaaaaaaag aaatgtttga aaacaaatat gcaataaaaa aatggaataa acaggaagta
    20581 aaaagagttt cagaaagtga atatgaacca attgaagggc aaaaagaatt tcttaccggt
    20641 gatcaaagct attgggataa caccggtaag atttttttct ttcaaagaga agtaaaatta
    20701 cctcaaaagt tagagaaaga aaatctttat cttcatcttg atatcagtgg tgaatgtact
    20761 ttatttatta atggagaggt atataggggt attaatgaaa aaaatataaa acttcccaaa
    20821 acagatagta atacttatca ctttaaaata ttagcaactc atgatgtaca cttatcggta
    20881 agacatcagc gtttatttaa tatcccctat cctcctcata tctttaggaa ctcattttta
    20941 tttaataaga ataaggatat tgaaaaactg tatgttttac taaaaaatat taaaaaaact
    21001 attgatgttt tagaatctaa aagagagcaa ctcattttgc aaaatttatt agaagatact
    21061 ttaaatcaga ttgacttttt tacaaataaa agaaaagaat tcctaacttc aattaataaa
    21121 tcatatcaat ctatagtaaa tgagctacaa aatatagaaa attctaatgt gggtaaagta
    21181 tttttgcttg ctcattccca tctagatttg gctttcaaat ggactattac agattctatc
    21241 cgtaaacttg aaaggactat tagcacgacc gttaatttaa tggattcata tgacaactac
    21301 tattttatcc aaagtcagtc tatcttatat gaatatctaa aagaatatta tcccaactta
    21361 tttcaaaaag ttaaagaaaa gtatcgggaa aataaattta tcgtggaagg ggctttctat
    21421 gtagaatcag acctcaatct cccccacggc gaatcattaa tccgacagat cctatacggt
    21481 aaaaaattct atcaagataa atttgataat aatactaacc tagcctggct gccggactgt
    21541 tttggcttca gtgccatctt acctcaaatt ctaaaaaagt cggaaattaa tttctttctt
    21601 accactaaac tacagtggaa tgaaactaac caattccctt ataacctttt caaatgggag
    21661 ggtatcgatg gctcacaaat ctcttcttac ctcctctccg atgaatatgg aggtaattta
    21721 gaaccaaaaa aaatatataa agcctgggaa aatagaaagc aaaaagattt agatgaagta
    21781 ttatccctat atggatttgg tgatggaggt ggaggaaaca acgaagtaca gttaaataac
    21841 tatgaggcct tagaaaagat accattccta cctaaaattg aaaccagtga tattcataat
    21901 caccttaata ccatatttaa taaaaatgaa ctccccacct ggaaagacga attatatcta
    21961 gaaaaacacc gcggtactta taccagtcag gccgagttga aaaaatataa tagaaaatta
    22021 gaattcaagc tgagaaatat ggaacttctc tattcatctg ccaaacttaa tggttatgaa
    22081 aaagaatata atttagaaaa gttatggaag atcttattaa aaaatcagtt tcatgatatt
    22141 ttaccgggct cctgtgtgca tgatgtatat gaagatgcag tggaagatta tgaacgatta
    22201 gatacaggtt tagaagatat taaaaatgaa ctttttgcat ttttctcaaa taaatataga
    22261 ttagatcata atcaatttat aatcttcaat cctctctctt ttgaaagaaa tgatttaatt
    22321 gaaataaaga aagaggagtt agaaaaagaa tatgataaag aatatgattc tgtgactatt
    22381 aataataata aatatccagt acaaaaatct aataatagtt attactttaa acccaatcat
    22441 ttgagccctt tatcttttac aaaattaaca ttttcaaata ataaaaatat caagtatgaa
    22501 aatcatttaa attcaagcca atactcaatt gaaaacaaat atataaaagt aactcttgat
    22561 aagaaaggac aaattagctc catatttgat aaagaaaaag aaatagaata tattcaagct
    22621 aaccaaaaag caaatcagtt gaaactatat gatgataaat ctacttactt tgatacctgg
    22681 gatatttcaa tttcagaaca aaataaaaga attatcgata atatcaaaga cataaaagtt
    22741 aaagcagccg gaccctattt tcactcaatt gaaataaaaa gaggttttta taactcttct
    22801 attagacaag aaatgatttt atatgaaaat aaacgcaaga ttgactttaa aactgaagtt
    22861 aactggcagg aaagtcaaaa attattaaaa accgcttttc ccattaaaat taaaaatagt
    22921 caggcccttt ttgacctgtc aatgggccat attgaaaggg caaactacaa aaatactagt
    22981 tgggaaagag caaagaaaga agtgcctgcc cataaatggg ttaacttaaa caataaagac
    23041 atcagtgtaa gcttattaaa cgactgtaaa tacggtcatg aaataaatga gaatgtaatt
    23101 aatcttactc ttttaaaaag cggtatcttc cctgatccta aggcagatat aggtaagcac
    23161 gaatttacct attctttatt gttaaacccc gattcacttg atttagttaa tttagaaaga
    23221 gaagctcttg ccatgaataa taaaccgatt tattgtaata taaataataa taatgaagaa
    23281 gataataaag tcaaaaaatt atttgatatt aaaaatgatc acatattact ggatagttat
    23341 aaaatatcag aaaataaaga tgggtttatt ctcagagtac atgaatttaa aggaataagt
    23401 aaccaattag aaatcaaatt tttaaataag attaaggaag tatatgaaac gaacctatta
    23461 gaaaaaaatg ttgaagaaat ttcaattgag aataataatt taactgttaa aataaatccc
    23521 tttgaaataa aaacatttag gattaagttc taagtgaaat aagaaaggct ccctcgcggg
    23581 agcctcttat ttatctcata ttaatttatt aagttaggat gactttagct tatcattcac
    23641 actatatgtt ttacggtcct tatactcttc ttgcttacca tcattccact ccgatactgg
    23701 agaaagataa ccaactaccc ttgaatacac ttcacacttt tgttttttac tcactctact
    23761 tccccctttt taatttctta tttttgcctt aatatcttta tttttaaata ctttaacaaa
    23821 atctttgagt tcatcttctg aaaaacttct tttctcttca taattagaac tattaacttt
    23881 ttctgacctg aaattttgca gataataatt ttttactcct aaaattgaat cagctatttt
    23941 ttccaatact tcttttgaat gcaaccccgg tacaacagtt gttcttaatt cataatcgat
    24001 atctgaatct aaaattaaat tcttgctttt ttttatatta tctattactt tcttatttaa
    24061 ttcaattccg caaaactttt catattcctc taaaggtcct tttatatcca ttgctacata
    24121 gtctattaaa tcttcatcga ttaatttttc taacaaatga taattagtac cattggtatc
    24181 taactttact aataaatcat attttttaat atctttaata aaattataaa tagaatcctg
    24241 aatcaaaggt tcgcccccag ttatcacaac accatcaatt aaatcacttc ttttatctag
    24301 atactcccaa aaataggctg tattcatata ttcattctca ttttctttgg gaattaactc
    24361 aggattatga cagtaaggac agtccatatt acacccctgt gtaaataata cggtcgaaat
    24421 gtaatcggga taatctatca aagaagtttt ctgaataccc gatattttaa tattattgtt
    24481 cactggcctt cgccctttct tccgcctcac acttgggaca atatttatgt tcgcccggta
    24541 gatagccatg tacaggacag atactaaaag taggagttat tgtataatat ggtaatttaa
    24601 aattcgctgc aattcttttt actaattttt tagtactttt gatgccgtta agcctttctc
    24661 ctaagaaacc atggaataca gtaccacccg taaattcaat ttgaaaatca tcctggattt
    24721 caagagcatc aaatatatca tcagtataac ctactggtaa atgagttgag ttagtataat
    24781 atggttctgc atcattttcc cttactctac tttcattagc cgcaattata tcattaccaa
    24841 atctttcttt atctaacctt gccaatctat aggaagtaga ttctgcgggt gtagcttcaa
    24901 ggttgtataa attctcggtt tcttcctgat attctcgcat tctacttttc attttttcca
    24961 ttacttcaat tgtaaattga tgggcctttt cttcagtaat attttccccc ataaagtttt
    25021 ctaaggattc attcatacca ttaattccaa ttgtattgaa atggtttttc caatactcat
    25081 caaacctctt tttaatgttt cttaaataaa atttagaata gggatacaat ccttgttcag
    25141 ttaagttttc tagtaccttt cgcttgattt ctaaactttc tttagctata tccattaatt
    25201 cataaactct attcatataa tcttctttat cttcagctag atatccaatc cgcggcatat
    25261 ttaaggttac tactccaatt gaacctgtca ttggattagc tccaaagagg cctccccctc
    25321 tttttcgcaa ctctctgtta tctaatctaa ggcggcagca
//
LOCUS       DATGCJ010000083         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_458321, whole genome shotgun sequence.
ACCESSION   DATGCJ010000083 DATGCJ010000000
VERSION     DATGCJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458321"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /gene="aroC"
                     /locus_tag="VKN64_04355"
     CDS             complement(<1..1096)
                     /gene="aroC"
                     /locus_tag="VKN64_04355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMA59504.1"
                     /translation="MFKYLTAGESHGKEITAIIKQVPAGLELKKDLIDQELIRRQGGY
                     GRGGRMDIEKDRVEITSGVRHGTTLGSPITLVIKNKDWENWQKEMSPEPADEKAKEAV
                     TKPRPGHADLAGALKYNTKDVRNILERASARETAIRTAVGSVCKQFLSEFDINIYSHV
                     IQIGPVKASSWEHLVVTDKKSGEYDQEKLGKYFKKVENSPLRCGNKEVEKEMIKLIDD
                     TKQKGDSIGGVIEILVFGLPVGLGSHVHWDEKLDGKLAQALISIQAIKGVEFGAGFKS
                     ARLKGSDVHDEIYYEQDSLQFYRSSNQAGGIEGGISNGEPLLIRMAMKPIPTLKNPLH
                     SVDLNSKETRFASKERSDVCAVPSASIVGEA"
     gene            complement(1097..1366)
                     /locus_tag="VKN64_04360"
     CDS             complement(1097..1366)
                     /locus_tag="VKN64_04360"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMA59505.1"
                     /translation="MEEELNELRKEIDKLNKELLKILSARGEIVKKIGRIKRKKDISL
                     VDSAREDEILKKIKSQNEGPYSSAQIGNIFQEIFKQSVELQKEDS"
     gene            complement(1368..2243)
                     /locus_tag="VKN64_04365"
     CDS             complement(1368..2243)
                     /locus_tag="VKN64_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HMA59506.1"
                     /translation="MKTYEQVTIIGTGLIGGSLAAALKRENICKRIVGVDVNSDSLKE
                     AEDKGIIEQGYLDIKKGVKSSDLIIIAVPVGNINSILNEIQEHAQQGTLIFDVGSIKG
                     DVIKQANQLVKVRPDLYFIGGHPMAGSEKSGVKWSDPDIFKDAPFILVPLKETPEKQV
                     ANLSDIINKIGGIVKIMGSEEHDKMVGYISHLPHLIAFALVNNIESCHNHQEIFELSA
                     GSFKDATRIAVSPPDLWADICLSNRAKLEPIFEEFLTELEQVKKMLTKGDKEGLLNYF
                     EKAGQCKSDLLQEEK"
     gene            complement(2243..3283)
                     /gene="aroF"
                     /locus_tag="VKN64_04370"
     CDS             complement(2243..3283)
                     /gene="aroF"
                     /locus_tag="VKN64_04370"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HMA59507.1"
                     /translation="MIIVMKTNSTNKQKDHVVEHLKKADYGVHMSEGIQRTIIGAIGD
                     EDDKEARMMKIRAIEGVEKVIPIMEPYKLTGKNFKPKKTVIDIDGVKIGGEEVVVMAG
                     PCAVENEEQIIKTAQAVKKAGAKILRGGAFKPRTSPYSFQGLKEEGLELLAKARDKTG
                     LKVVTEVIDVNKIDLVNEYADIIQIGARNMQNYPLLKAVGKLDTPVMLKRNMSSTIKE
                     WLLAAEYIMKEGNYQVILCERGIRTFSTNTRNTLDLSSVPLLHELSHLPIVVDPSHGT
                     GKWSLVSPMARAAVASGADGLLVEVHPNPEAALSDGAQSLTFDNFNQLMVEVRKMAEV
                     LKRKVGTQEVLA"
     gene            complement(3288..>4121)
                     /gene="hisC"
                     /locus_tag="VKN64_04375"
     CDS             complement(3288..>4121)
                     /gene="hisC"
                     /locus_tag="VKN64_04375"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMA59508.1"
                     /translation="NGSDEIIDHIMTVFVEKGDEVILADPSFIKYKLAVQASGGISKE
                     IPLTEDYRHDLDRMAEAVTEKTKVIFICDPNNPTGTTVGRKKLLDFIDEVPSDILLVV
                     DQAYYEYVTETDYFKGLELIDQYSNLILLRTFSKIYGMAGFRVGYGIANPQLVELLNR
                     VRSPFNVNKVAQIAAVAALNDQQHVQNSRACNEKGKNYLYNSLDELGLQYIPTQGNFM
                     LINTEQNAVEVFEKLMQQGVIVRPGNIFGLPTWIRVTIGQKEDNQEFIKQLKNIISKG
                     A"
BASE COUNT         1073 a          957 c          682 g         1409 t
ORIGIN      
        1 ctgcttctcc tacgatactg gccgaaggga cagcacaaac atcacttctc tctttactgg
       61 caaatcgagt ttctttactg tttagatcaa ctgaatggag tgggtttttt aaggtaggta
      121 taggtttcat ggccattctg attaataaag gttccccgtt tgaaattccc ccttcaatac
      181 cgcccgcttg attacttgaa cggtaaaact gtaaactatc ttgttcataa tagatctcat
      241 catgaacatc tgatccctta agtctagcac tcttaaatcc ggccccaaac tccacacctt
      301 taatcgcctg gatactgatc aaagcctgag ctaactttcc atccaatttc tcatcccagt
      361 ggacatggct gcctaatcca accggtaaac caaaaaccaa tatttcaatt actcctccga
      421 tggaatcgcc tttttgtttt gtatcatcga tcagtttgat catttctttt tccacttctt
      481 tgttgccgca gcggaggggc gagttctcta cttttttaaa atatttacct aacttttcct
      541 ggtcatactc tccagatttt ttatctgtta caactaaatg ttcccaggaa gaagccttaa
      601 ccggaccaat ctgtattaca tggctataaa tattaatatc gaattcagat aaaaattgtt
      661 tgcagaccga tcctaccgca gtccgaatgg cagtctcccg ggcactggcc ctttctagaa
      721 tatttcttac atctttcgta ttgtacttaa gggcaccggc caaatcagca tggcccggac
      781 ggggtttggt tacagcttcc tttgctttct catcagcggg ctctggtgac atctcttttt
      841 gccagttttc ccaatccttg ttttttataa caagtgtaat agggctgccg agggtggtac
      901 cgtgcctaac tcccgaggtg atctcaaccc ggtctttttc aatatccatc ctaccgcccc
      961 gaccataacc gccctgacgg cggatgagtt cttgatcaat taaatctttt tttaactcta
     1021 aaccggccgg tacctgtttt atgatagctg taatctcttt accatgtgat tcaccggctg
     1081 tcaaatattt aaacatttat gaatcctcct tctgcaattc caccgattgc ttaaaaatct
     1141 cttgaaaaat attgccgatt tgggctgaag aatagggacc ttcattttga ctttttatct
     1201 tctttagtat ttcatcttct ctagcgctgt ccacaagaga aatatctttt tttcgtttta
     1261 tccgaccgat cttttttact atttcgccgc gagccgatag aatttttaaa agttctttgt
     1321 ttaatttatc gatttctttt cttaattcat ttaattcctc ttccatctta tttctcctcc
     1381 tgtagtaaat cagatttaca ctggcctgct ttttcaaaat agttcaacaa tccttcttta
     1441 tctccttttg tcagcatctt tttaacttgt tctaattctg ttaaaaattc ttcaaagatc
     1501 ggctctaact ttgctcggtt agataaacag atatctgccc agagatccgg tgggctgact
     1561 gcaattcggg ttgcatcttt aaagctgccg gctgaaagtt caaatatttc ctgatgatta
     1621 tgacagcttt ctatattatt gaccaaagca aatgctatca aatgaggtag atggctgata
     1681 tatcccacca tcttatcatg ctcctctgaa cccatgattt tgacgattcc accaatttta
     1741 ttaattatat ctgacaaatt agcaacctgt ttctcgggtg tctcttttaa gggaaccaag
     1801 ataaaaggtg catctttgaa tatgtcagga tcagaccatt taaccccact tttttctgag
     1861 ccggccatcg ggtgaccgcc gataaagtaa agatccggtc ttaccttaac gagttggtta
     1921 gcctgtttaa taacatcacc tttgatcgaa cctacatcaa atatcaaagt accttgttgg
     1981 gcatgttctt gtatttcatt taaaatagag ttaatatttc caaccggaac agctataatt
     2041 attaggtctg aacttttaac acctttttta atatcaagat atccttgttc tatgatacct
     2101 ttatcttcgg cctcttttaa agaatcagaa ttaacatcta ccccgacaat cctcttgcaa
     2161 atattttctc tttttaaagc tgcggccagg gaaccaccaa ttaacccggt accgattatg
     2221 gttacctgct catatgtttt catcaggcta gaacctcctg ggttcctacc tttcttttta
     2281 ggacctctgc catttttctt acttccacca ttaattgatt aaagttgtca aaggtaaggg
     2341 actgggcccc atctgaaaga gcggcttccg gatttggatg gacctctact aacagaccgt
     2401 ctgctccact ggcaacagcg gctcgggcca tcggtgagac caaactccat ttaccggtcc
     2461 cgtggctggg atctaccaca attggtaagt ggcttaactc atgtaataga ggtactgaac
     2521 ttaaatcaag ggtattcctg gtattggtac tgaaggtgcg aatacccctt tcacatagga
     2581 taacttgata gtttccttcc ttcataatat attcagccgc cagtaaccat tcttttatgg
     2641 tagaagacat attccttttt aacattacag gggtgtccaa ctttccaact gcctttaata
     2701 aggggtaatt ctgcatgttg cgggccccga tctgaataat atctgcatat tcattgacca
     2761 gatctatctt attaacatct atcacttcag tcacaacctt taagccggtt ttatcacggg
     2821 ctttggccaa taattctaaa ccttcttctt ttaaaccctg aaaactatag ggggaagttc
     2881 tgggtttaaa agctccaccg cgtaagatct tggcacctgc ctttttaacc gcctgggcgg
     2941 tttttataat ctgttcttca ttttcaactg cacagggtcc ggccatcaca actacttctt
     3001 cacccccaat ttttactcca tcaatatcta tgactgtctt ttttggtttg aagtttttac
     3061 cggttaactt gtagggttcc ataatgggaa taaccttttc taccccttcg atagccctta
     3121 ttttcatcat acgggcttcc ttatcatcct catctccaat tgctccaatt atggtccttt
     3181 gaataccttc tgacatatgt acaccataat ctgccttctt taaatgttca actacatgat
     3241 ctttctgctt attggtacta ttggttttca taacaattat catattatca ggctcctttc
     3301 gagattatat ttttaagttg tttaataaat tcttgattgt cttctttctg accgatagta
     3361 accctgatcc aggtgggtaa accaaaaata ttgccgggcc ttacgataac tccctgctgc
     3421 ataagttttt caaaaacttc aactgcattc tgctcagtat ttatgagcat aaagtttccc
     3481 tgggtaggta tatattgaag tccaagctca tctaaggagt tatataaata gtttttccct
     3541 ttttcattac aagctctgct gttttgcaca tgttgttggt catttagggc ggcaacagcc
     3601 gctatttgag ctaccttgtt gacattaaat ggacttcgaa ctcgatttaa tagctcgacc
     3661 agctgagggt tagctatgcc ataaccgacc ctaaagccag ccattccata aatcttggaa
     3721 aatgtcctta aaagaattaa atttgaatac tgatctatca attccaatcc tttaaagtaa
     3781 tcagtttctg tcacatattc atagtaagcc tggtcaacaa caagcaaaat atcagatggg
     3841 acctcatcaa taaaatctaa taactttttc ctgcctacag tagtcccggt cgggttattg
     3901 ggatcacaga taaatattac tttggttttt tctgttactg cctcagccat cctgtccagg
     3961 tcatgcctat aatcttctgt caggggaatt tctttactaa tccctccact ggcctgaacg
     4021 gccaatttgt attttataaa ggaaggatca gctaaaatta cttcatctcc cttctcgaca
     4081 aaaaccgtca ttatatgatc gattatctca tccgaaccat t
//
LOCUS       DATGCJ010000084         9519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_101521, whole genome shotgun sequence.
ACCESSION   DATGCJ010000084 DATGCJ010000000
VERSION     DATGCJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101521"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..840
                     /locus_tag="VKN64_04380"
     CDS             151..840
                     /locus_tag="VKN64_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HMA59509.1"
                     /translation="MLEEIKKRVLTANLSLPKYDLVTFTWGNVSEIDRENDLVVIKPS
                     GVPYEELTEEKLVVVDLKGEIVEGDLNPSSDTATHLELYKNFPDIGGIVHTHSPWATS
                     WAQAGKGIPCFGTTHADHFYGEIPCTRKMSNPEIEEEYEKNTGRVIVETFDQIDENEI
                     PAVLVNSHGPFVWGEDAMAAVKNSVVLEEVAKMAYRTVQLDDHRSSINPTLLDKHFLR
                     KHGKESYYGQD"
     gene            877..2553
                     /locus_tag="VKN64_04385"
     CDS             877..2553
                     /locus_tag="VKN64_04385"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HMA59510.1"
                     /translation="MSKKYSIGIDFGTKSGRAVLVDVDTGEEVTTSVLEYPNGVIDER
                     LPLEDEEVELGIDWALQDPNDYMKVIYETIPEVIQKAKIAPEQVIGLGIDFTACTMLP
                     VDEKGEPLCNKDEFKNKPNAWVKLWKHHAAQPHANKLNEVAKKRGEDFLNRYGGKISS
                     EWLFPKIWQTLDEAPEIYEAADRFMEAADWLIMQLTGVEKRNISTTGYKAIWSKESGY
                     PDKEFFKALDPRLENVVEEKLSNDIYDLGGKAGNLKEEIAKKINLTPEVAVAIANVDS
                     FVSVPAVTVSEPAKMVMVMGTSICHMVIDNKEQFVPGMTGVIRDGILPGFYGYEAGQS
                     AVGDIFEWYVSEGIPAYFAKEAKENDKNIYEYLEEKAAELKPGESGLLALDWWNGNRS
                     VLVDADLTGMILGLHLNSKPGEIYRALIEATAFGTNKIIKTFEASGVEINELYGCGGL
                     VKKNETLMQIYADVTSKPIKIAASEQTPALGSAMHGAVAAGSKKGGYDDINEASKKMA
                     HLEDKIYQPNQENVKIYEKLYQEYEGLHDYFGRGENDVMKRLKNIKQQIE"
     gene            2602..3909
                     /locus_tag="VKN64_04390"
     CDS             2602..3909
                     /locus_tag="VKN64_04390"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HMA59511.1"
                     /translation="MIEDLLNSSFTCSCGKEHHINIKEIIVGRDILNNIPKTLPALTD
                     KKKVLLVSDNNTYQAAGEKASRILKEAGFKVNNLILTGNQVKPNPTNLFKIIREYTED
                     QYIIACGSGSINDLTGYASYKMGEPYSVLATAPSMDGYASSVASITAEGVKQTYNTAP
                     AEAIFADIEVLRKAPLDLILAGYGDLLGKTTSLLDWKLADILFNEYYCEKAVSIVKNE
                     LKNIIQLLKKGNIRAAENIETLIKGLIYSGIAMLMVDSSRPASGSEHHISHYLEMFGE
                     KENIDLPYHGIKVGIGTLFTTSFYLKLKKLDFSKLEIRLNKDKREEEIKKIYGDRAQP
                     ILDNLYERWNKESITKDEIKNKEYDIKNLIDHNIEIIKKARKILTENNFLQADKTENI
                     SKKLLKNAIRYGFEIRTRYTVTTFLNQVEKLDEFVDALLEYYH"
     gene            4092..5249
                     /locus_tag="VKN64_04395"
     CDS             4092..5249
                     /locus_tag="VKN64_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HMA59512.1"
                     /translation="MGMTSRQRINTALNHKEPDRIPVDLGSTIVTSICKGAYIDLMKE
                     LDFKIEENEVEIMDVVQQLPFLDERLLNWAEVDAVPLMTNGPSDWELEIKEERDYYTF
                     VDQWGAKLYRPKDGFYYDYREFPIKESSLKALEEMAWPEPNDPARMKGIKEKAERLYK
                     ETDYAIVGSPIFGGGIFEHPARVRGMEEFLMSTVNNKKFAEAIMEKFTQLYIQATENF
                     LSEVGEYIDVFAYWDDVGMQNGPMIRPSFYQKYVKPRQKRLFDTVRKMSDAKIFLHTC
                     GASSQFIPDFIDIGVDILNPIQVSAKGMDPKKLKKEYGKDLTFWGGGVDTQNVLPFGS
                     KKDVRKEVKNRIEELAKDGGFVFAAVHNIQNMVPPANIIEMFETLKKNWEY"
     gene            5470..6468
                     /locus_tag="VKN64_04400"
     CDS             5470..6468
                     /locus_tag="VKN64_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMA59513.1"
                     /translation="MFKKVSIILLAVSLLIGLMAFPAMAQEESDDPVEIYFFPGGSPG
                     GTFATVVYNGAKKAEEILGDRVKVHYRWSEWSPQQMVSQFQQAVAANPDGIAIMGHPG
                     DEAYRPFVEEAREKGIIVTSQNTTLPELESKYKGEGFGYVGQELYPSGQTLGEGCVAR
                     FGLGEGDKALVWGLKSKPTRGQRTQGVIDALEDAGLEVDYMEISAEVDKDASNGIPVI
                     VGYLQSNPDCDLIVTDHGNLTSSLQTYIESAGLGPDDIIGAGFDLSAATAEGIKNGYT
                     DLVLDQQPFLQGFLPIYQIYLTEKWGFSGLHIDTGAGLVHEGNIDMIAPLAEEGIR"
     gene            6581..7333
                     /locus_tag="VKN64_04405"
     CDS             6581..7333
                     /locus_tag="VKN64_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA59514.1"
                     /translation="MSLVNMKNIHKHYGGVTALRDINFEVGSNEVVGVMGDNGAGKST
                     LIKIITGLHPFDKGEMYIKDKKIDPRKYSVKKAHEIGIETVYQEQALAEKQSLWRNIF
                     LGRQKTNKLGFIKVKEEKEETNEIMRELMGFTGIGANPDSNIKTLSGGEKQGVAIGRA
                     MHFDADLVILDEPTMALSLQEVEKVLNFIRDIKKRGKACIYISHNVANVYPVSDRFVV
                     LDRGEIVGRYEREKISLQDLNKELVELTKVEG"
     gene            7342..8313
                     /locus_tag="VKN64_04410"
     CDS             7342..8313
                     /locus_tag="VKN64_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59515.1"
                     /translation="MNSNSKLLSKEKILKYRTQVGLIGIAIIVLLLFMVTSPDVFLSP
                     QIYKSFLSTVPFVGVMALGLTMILAMGEIDLSFPSVMALSGFVFAIVFKTTGSIFIAL
                     IATIATGAVVGLLNGLLITKIGIPSIIVTIGMQFFIRGFSNILSQGRAYPMDLSSSIF
                     YKIFAGNIFGLPSQSYWFIGLGIFLWFILFRHKFGEHILFTGDNEDATKMMGINVDRV
                     KIATFILMAVLASFAGVLDIFRMRTWWPTMGEGYLMMVVATVFVGGTSMHGGDATILG
                     TLIGAFLIGSLEAGIVAAGLSGFWTRFIYGLLIIAAVTLHTVLKERK"
     gene            8416..9519
                     /locus_tag="VKN64_04415"
     CDS             8416..9519
                     /locus_tag="VKN64_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMA59516.1"
                     /translation="MANEYLPKYIKIKNDIEDMINEGKIKPGSKLPTENELSAKYGVS
                     RHTVRKALNILRQEGLLYRKQGVGTFYKNNSNKTTKNIGFISISLHAYIFADMLSSVD
                     KILYENGYQILLGNSMDNHKREKEILKEFIKKNVDGLIIEPAKSARNYPNTSLLKRFV
                     NNNIPVTILDSKFEEDNFNNITVDDVHGGILATEHLLEMGHKNIAMIYKGLHKPALGR
                     FRGYKQALKKHNIPVYNNYVKQYLNSEFEDRTNFKKEIEKITKELIKADPQPTAIFCF
                     NDQVAILVKEILQKQGFEVPADISIVGFDNSKQVNLENITSVAHPKEKVGEKAAKITL
                     ERINDGKFEYCEDVMFKPKLVKRGSVKRIQKAG"
BASE COUNT         3328 a         1494 c         1931 g         2766 t
ORIGIN      
        1 ttggcttttt tgtttataat tacaactaaa ataagattaa aagcggctat ttaaattaat
       61 attctactta tatttttgaa ttttgaaaaa attaaaaaag ggttaaagtc tctttttgtt
      121 taataatatg ttataaagga ggttctaaaa atgctggagg aaataaaaaa aagggttcta
      181 acagccaatt tatcactacc aaaatatgat ctagttacct ttacctgggg taatgtcagc
      241 gaaattgacc gtgaaaatga tttagtagta attaaaccca gtggggtacc ctatgaagaa
      301 ttaacggaag aaaaattagt ggtcgttgac cttaaagggg aaatagtgga aggtgattta
      361 aatccttctt cagataccgc tacccacctt gaactatata aaaactttcc agatattggt
      421 ggtatagtac acacccattc gccctgggca accagctggg ctcaggccgg taagggaatc
      481 ccctgttttg gtactaccca tgctgatcac ttttatggtg aaatcccctg tacaagaaaa
      541 atgtctaacc cggaaattga agaagaatat gaaaagaata ccggccgggt tatcgttgaa
      601 acctttgatc aaatcgatga aaatgagata ccagccgtat tagtaaatag tcatggcccc
      661 tttgtctggg gagaagatgc tatggccgca gttaaaaact ctgttgtctt agaagaagtt
      721 gctaagatgg cctatagaac tgtgcaatta gatgaccata gatcttctat taatcctacc
      781 ctacttgata aacactttct tagaaaacat ggtaaggaat catattatgg ccaagattaa
      841 tttttgatta aaatatgaaa actaggaggg acaaagttga gtaaaaaata ttcaattgga
      901 attgatttcg gcacaaaatc aggtagagca gttttagttg atgtagatac gggtgaagaa
      961 gtaactactt cagtccttga atatcctaat ggggttatag atgaacggct acctttagaa
     1021 gatgaagaag ttgaactggg tattgattgg gctttacagg accccaatga ttatatgaag
     1081 gttatctatg aaaccatacc tgaagttatt cagaaagcaa aaattgcccc cgaacaggta
     1141 attggactgg gtatcgattt tacagcctgt actatgctgc ctgttgatga aaaaggtgaa
     1201 ccactttgta ataaagatga atttaaaaat aaaccaaatg cctgggttaa attatggaaa
     1261 caccatgctg cccaacccca tgctaataaa ttgaatgaag ttgctaaaaa aagaggagaa
     1321 gactttttga atagatatgg gggaaagatc tcttcggaat ggctatttcc taaaatctgg
     1381 cagactttag atgaggctcc tgaaatctat gaggcggccg accgttttat ggaagctgct
     1441 gactggttaa taatgcagct aaccggggta gaaaaaagaa atatttcaac taccggttac
     1501 aaagcaatct ggagtaaaga aagcggttat cccgacaaag aattctttaa agctttagat
     1561 ccccgcttag aaaatgtagt agaagaaaaa ctttcaaacg acatttatga tttaggcgga
     1621 aaagcaggta acctaaaaga agaaattgcc aaaaaaatca acttaacacc tgaggtagca
     1681 gttgccatcg ctaatgttga ctcttttgtt tcagttccgg ctgtaacagt atctgaaccg
     1741 gctaagatgg taatggtgat gggaacttca atctgtcata tggtaattga taacaaggaa
     1801 cagtttgtgc cgggcatgac cggggtaata agagatggta tcttaccggg cttttatggc
     1861 tatgaagcag gccaatctgc tgtaggtgat atctttgaat ggtatgtaag tgaaggtatt
     1921 ccagcctact ttgcaaaaga agcaaaagaa aatgataaaa atatctatga atacttagaa
     1981 gaaaaagcgg ctgaactaaa accgggtgaa agtggactat tagctcttga ttggtggaac
     2041 ggcaaccgtt cagtcttagt tgacgccgat ttaaccggta tgatcttagg tcttcacttg
     2101 aattcaaaac caggtgaaat atatagggca ttaattgaag ctactgcttt tggaaccaat
     2161 aaaataatca agacctttga agccagtgga gttgaaatta atgagctata tggctgtgga
     2221 ggtttagtaa aaaagaatga gacattaatg cagatctatg ctgatgtcac tagtaaaccg
     2281 atcaaaatag ccgcctctga acaaactccc gctctgggtt cagctatgca cggcgcagtt
     2341 gcggcaggta gtaaaaaggg tggatatgat gatataaacg aagcaagtaa aaaaatggcc
     2401 catcttgaag ataaaatcta tcaacccaat caagaaaatg tcaaaatata tgaaaaatta
     2461 tatcaagaat atgaagggct gcatgactac ttcggtcggg gagaaaatga tgtgatgaaa
     2521 aggttaaaga atatcaaaca acaaattgaa taattgaatt aaaagataaa gtaaacttta
     2581 tttcaaaagg agagttttct aatgatagaa gatttactaa acagttcttt tacctgtagt
     2641 tgcggtaaag aacaccatat aaatattaaa gaaataatcg ttggcagaga tatattaaat
     2701 aatataccaa agactcttcc tgctttaact gataaaaaga aagtattatt ggtcagtgat
     2761 aataatactt atcaggcggc aggcgaaaaa gccagccgaa ttttaaaaga agctggcttt
     2821 aaagtaaata acttaattct tacaggtaat caagtaaaac caaaccctac aaatcttttt
     2881 aaaataatca gggaatatac cgaagatcaa tatatcattg cctgtggctc aggttctatc
     2941 aatgatctaa ccggatatgc cagttataaa atgggggaac cctattcagt attagccact
     3001 gccccatcta tggatggtta tgcctcttca gttgcttcta taacagccga aggtgtaaag
     3061 caaacctata atacagctcc tgctgaagcg atctttgctg atattgaggt cttaagaaaa
     3121 gcaccacttg atctaattct ggccggctat ggtgatctac tgggtaagac cacctcatta
     3181 ttagattgga aactggctga tattttattc aatgaatatt attgtgaaaa agcagtcagt
     3241 attgtcaaaa atgaattgaa aaatatcatt caacttctca aaaagggaaa cataagagca
     3301 gctgaaaata ttgaaacttt gattaaagga ttaatctatt ctggtattgc gatgcttatg
     3361 gttgatagct cacgaccagc ttccggtagt gaacaccata tttctcatta cttggaaatg
     3421 ttcggtgaaa aagaaaatat agatctacca taccacggta ttaaagtagg aatagggaca
     3481 ttatttacca cttcatttta tcttaaatta aaaaaactgg acttttccaa actggagatt
     3541 agattgaata aagacaagag agaagaagag ataaaaaaga tctatgggga tagggcccag
     3601 ccaattctag ataatctata tgaacgctgg aataaagaat ctatcacaaa agatgaaata
     3661 aaaaataagg aatatgatat aaaaaatcta attgatcaca atattgaaat aattaaaaaa
     3721 gcaagaaaga tactaacaga aaataatttt ctgcaggccg ataaaactga aaatatctca
     3781 aaaaaactgt taaaaaatgc tataaggtac ggctttgaaa ttagaacaag atatactgta
     3841 acaacatttt taaaccaggt ggaaaaatta gatgaatttg ttgatgcctt actagaatat
     3901 tatcattaaa ccagcataaa tattttaaaa agtaataaag ttgtaaattt tcagaataat
     3961 ttaaactttt atgaaggagt tttaaattta atctataatg aataaaatag aacatatata
     4021 ttttttgaat aaatttgtct gcacaaccat acaaaactat ataagagatt tattacggag
     4081 gaggagctat catgggaatg acttcccgac aaagaataaa tacagcctta aaccataaag
     4141 aaccagaccg gattccggtt gatcttggtt caactattgt aacttcaatc tgtaagggtg
     4201 cttatattga cctaatgaag gaacttgact ttaagattga agaaaatgaa gtggaaataa
     4261 tggatgtagt acagcagtta ccttttttgg atgaaaggtt actaaactgg gctgaagttg
     4321 atgcagtacc cttaatgaca aatggtcctt ccgattggga attggaaatt aaggaagaaa
     4381 gagattacta tacatttgtt gatcagtggg gagctaagct atacaggcct aaagatggat
     4441 tctattatga ttaccgggag tttcccatca aggaatcttc tcttaaagcc ctggaagaaa
     4501 tggcctggcc tgaacccaac gatcccgccc gtatgaaagg tatcaaagaa aaggctgaaa
     4561 gattatataa ggaaaccgac tatgccattg ttggttcacc catttttggc ggcggtatct
     4621 ttgaacatcc cgcccgggtg aggggtatgg aagagttttt aatgtcgaca gttaataata
     4681 agaagttcgc ggaagctatt atggaaaaat tcacccagtt atatattcag gccactgaaa
     4741 acttcctatc agaagtagga gaatatatag atgtatttgc ttactgggat gatgttggca
     4801 tgcaaaatgg gcccatgatc cggcctagtt tttatcaaaa atatgttaaa ccccggcaaa
     4861 aaaggctttt tgatacggtt cgtaaaatga gtgatgcaaa aatatttctt catacctgtg
     4921 gggctagttc tcaattcata cctgatttta ttgatattgg agtagatata ttgaatccaa
     4981 tccaggtttc agcaaaaggt atggatccca aaaagttaaa aaaagaatat ggaaaagatc
     5041 taaccttttg gggaggggga gttgataccc aaaatgtcct tccctttggc agtaaaaaag
     5101 atgtgagaaa agaagtcaaa aacaggattg aagagttagc taaagatgga ggctttgtat
     5161 ttgcggctgt acataatata caaaatatgg ttccacccgc caatataatt gaaatgttcg
     5221 aaaccctaaa aaagaattgg gaatattaat actcaaaaat aacagctaat atttccaaat
     5281 aatttttact atatttgtct atacaaacat acattagatt taaatattat agagtttgta
     5341 gtaattaata gacatcaaga gaaagatacc attattttta gcttaaccat aaagggggtg
     5401 tagattgact tactaaccac taattgattg gcattaggag acttttaata aaaaagggag
     5461 gtttattaaa tgtttaaaaa ggtaagcatt atcttgctag ctgtttcatt attaatcggc
     5521 ctcatggctt ttccagcaat ggcccaggaa gaatctgatg atccggtaga aatatatttc
     5581 ttccccggtg gttctccagg tggtacattc gcaactgttg tctataatgg cgctaaaaag
     5641 gccgaagaga tcttaggtga tagagttaaa gttcactaca gatggtcaga gtggagtcca
     5701 caacaaatgg tttctcagtt ccaacaagct gttgctgcta atccagatgg gattgctatc
     5761 atgggtcacc cgggcgatga agcatacaga ccatttgtcg aagaagcaag agaaaagggc
     5821 attattgtta ccagtcaaaa tacaacctta ccagaattag aatccaagta taaaggtgaa
     5881 ggttttggtt atgttggtca ggaactatat ccttccggtc agaccctggg agaaggttgt
     5941 gttgcaagat ttggtctagg tgaaggagac aaagccttag tatggggttt aaaatcaaaa
     6001 ccaacccgtg gacaaaggac tcaaggtgta attgatgcac ttgaagatgc aggtttagag
     6061 gtcgactaca tggaaatttc agccgaagtt gataaagatg cttccaacgg tattcccgta
     6121 atagttggtt atctacagtc taatccagat tgtgacttaa ttgttactga tcatggtaat
     6181 ctaacctcat ctctacaaac atatatagaa tcagctggct taggaccaga tgatattatt
     6241 ggagcaggat ttgacctttc cgctgcaact gcagaaggta ttaaaaatgg ttatacagac
     6301 ttagtattag atcagcagcc cttcttacaa ggtttcctac caatttatca aatttacctt
     6361 acagaaaaat ggggattctc aggcttacac atcgatacag gtgctggctt agtacatgaa
     6421 ggtaacatag acatgatcgc tccactagca gaagaaggta taagataaat taacatatag
     6481 aacactaaga agtgatgggg cctttttagg ccctgtcact tataatataa ctagttaatt
     6541 caattaatat atacacctac ttattaggag gttattaact atgagtttgg ttaatatgaa
     6601 aaacatacat aaacattatg gtggtgtaac ggcccttaga gatattaatt ttgaagtagg
     6661 ctccaatgaa gttgtgggag ttatgggtga taacggggca ggtaaatcca ctttgatcaa
     6721 aattatcacg ggcttacatc cctttgataa aggtgaaatg tatataaaag acaaaaagat
     6781 agatcccaga aaatactcgg ttaaaaaggc ccatgaaatc gggattgaaa ccgtttatca
     6841 ggaacaggcc ttagctgaaa agcagtctct ctggcgtaat atctttttgg gtcgtcaaaa
     6901 aacaaataaa ttgggcttta ttaaagtaaa agaagagaag gaagagacca acgaaataat
     6961 gagagagttg atggggttta caggaattgg tgctaaccct gattcaaaca taaaaaccct
     7021 ctctggtggt gaaaaacagg gtgttgcaat cggtcgtgct atgcactttg atgccgattt
     7081 agttatcctc gatgaaccaa ccatggcctt atccttacaa gaagttgaaa aggttcttaa
     7141 ttttattcgc gatataaaaa agagagggaa ggcatgtatc tatatttcgc ataatgtagc
     7201 aaatgtttac cctgtatctg accgttttgt agttttagat cggggcgaaa ttgtcggtag
     7261 atatgaaaga gaaaagattt ctctacaaga tctaaataaa gaattagtcg aacttactaa
     7321 agtagaaggt taggtgatat aatgaattct aactctaaat tattatctaa agaaaaaata
     7381 ttaaaataca ggacccaggt tggccttata ggtatagcta ttattgtatt actattgttt
     7441 atggtaacaa gtccagatgt ctttttatca ccacagatct ataaatcatt cctctctact
     7501 gtgccttttg taggtgttat ggctttaggc ttgaccatga tcttagcgat gggagagata
     7561 gatctttcat tcccttctgt aatggcccta tcaggctttg tctttgcaat agtttttaaa
     7621 actaccggca gtatatttat tgctctaatt gcaactattg caaccggggc ggtggtcgga
     7681 cttttaaatg ggctcttgat aacgaaaatc ggtatcccct cgatcatcgt tacaattggt
     7741 atgcagtttt tcatcagagg attttcaaac atactatcac agggtagagc atatcccatg
     7801 gacttaagta gcagtatctt ttataagatc ttcgccggta atatatttgg gttaccttct
     7861 caatcttact ggtttatcgg cttaggtatc tttttatggt ttatcctatt taggcataaa
     7921 ttcggtgaac acatcctttt taccggtgat aatgaagatg ccaccaagat gatgggtatt
     7981 aatgttgacc gggttaaaat tgctactttt attttgatgg cagttttggc ttcctttgcc
     8041 ggtgtactgg atatttttag aatgagaact tggtggccga ccatggggga aggatactta
     8101 atgatggttg tcgctactgt atttgttggt ggaacttcca tgcatggtgg agatgccact
     8161 atcctgggta ctttaattgg tgccttttta attggttctc tcgaagctgg tattgtagca
     8221 gctggcttat caggcttttg gacccgtttt atttacggtc ttctcatcat agctgctgta
     8281 acacttcata ctgtacttaa agaaaggaaa taactatctt atataagaaa gaaaaatata
     8341 ctcttgttta aatattctat ctagatttac agaacgacta tatttgatgt aaaatgactt
     8401 tgaaggtgat aatttatggc aaatgaatac ttaccaaaat atataaaaat caaaaatgat
     8461 attgaagata tgataaacga aggcaaaatt aaaccgggta gtaaattacc aactgaaaat
     8521 gagttatcag ccaagtatgg agttagtagg catactgtac gtaaggcttt aaacatatta
     8581 aggcaggaag gactattata tagaaaacaa ggggtaggca ctttctataa gaataattcc
     8641 aataaaacaa ctaaaaatat tggatttatt agtatcagtc tgcacgccta tatctttgcc
     8701 gatatgttaa gctcggttga taagattctt tatgagaatg gctatcagat cttattgggt
     8761 aattcgatgg acaaccacaa aagagaaaaa gagatcttaa aagaattcat aaaaaagaat
     8821 gtggatgggc tgatcataga acctgctaaa agtgctcgaa attaccccaa tacttcctta
     8881 ttaaagaggt tcgtaaataa taatattccc gttacaattt tagacagtaa atttgaagaa
     8941 gataacttta ataatattac tgtagatgat gtacacggcg gtatcctggc aaccgagcac
     9001 ctcttagaga tgggtcataa aaacattgct atgatatata aaggtctaca taagccggct
     9061 ttggggcgtt ttaggggcta taaacaggct ttaaagaagc acaacatacc tgtctataat
     9121 aattacgtaa aacagtactt aaactctgag tttgaagaca gaactaattt caaaaaagaa
     9181 atagagaaaa ttaccaaaga attgatcaag gctgaccccc aaccaacagc tatattttgc
     9241 tttaatgatc aggttgccat ccttgttaaa gaaatactgc aaaaacaagg atttgaagta
     9301 ccagccgata tttcaattgt tggttttgat aattccaaac aagttaattt agagaatata
     9361 acctctgtgg cccacccaaa agaaaaagta ggagaaaaag cagccaaaat aaccttagaa
     9421 agaattaatg atggtaaatt tgaatactgt gaagatgtta tgtttaaacc caagttagta
     9481 aagagaggtt ctgtgaaaag aatccaaaaa gcggggtga
//
LOCUS       DATGCJ010000085         9852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_101827, whole genome shotgun sequence.
ACCESSION   DATGCJ010000085 DATGCJ010000000
VERSION     DATGCJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101827"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VKN64_04420"
     CDS             complement(<1..659)
                     /locus_tag="VKN64_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMA59517.1"
                     /translation="MTNYKYYTNNSIKATTGLVAAYNMIPISGQKIVDISGNGNDASR
                     NGTPLSTKEGVKFSGANDEYYGASSISPSFDGGKQVSIAVRFKPDNVSGTNKYIVSIP
                     ESTVGNNGINIRQNNDAIITDLLQNGSGDSVEATAVLTTNKWYTAILTFDGSTQKLYV
                     NGSLADSSDTAYALEAASDEINIGDFGTFDGAFEGEISDVRIYNRALSEQEVKDYHNS
                     F"
     gene            complement(672..2027)
                     /locus_tag="VKN64_04425"
     CDS             complement(672..2027)
                     /locus_tag="VKN64_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMA59518.1"
                     /translation="MGKIFEFDARKGSLINEVDNSIGVLTVGDGGFKKTEKGLAMNFD
                     GSSTNIEYSEIELTSGDFTIEAIVKFNELNVSIDTKTILSLYQGGDNSIALNTEFGGD
                     KIQFFSRGGDSFIGSTSMKIGTYYHIIIVNEGGIVSIYLNGTFDGSGTPSISYPTEVC
                     TLYVGTERSSYYHNGYIVKCVLHNNPFSLTDIHNSYNDFLRSSPITKGNASTKYVLSK
                     PNDLSHERNTHYTGDEYIQVMEFTDNASDTSSLKLNNGGDFAVVDWTGNSDFQEISTS
                     SETESNVIPSTVKIFAKNGVLTYFESADNDFDFNLNILPSSLTDFRCYGNNTVSGDLA
                     GLPSTLTYFRCDGNNIVSGDLADLPSTLTYFICYGSNTISTYTSGHTFNSGIDYFLLL
                     PAAGYGLDSTEVDNLLIDLDNSGMSSGTIDISGNNAARTSASDAAVTSLEGKGVSVTT
                     N"
     gene            complement(2028..3173)
                     /locus_tag="VKN64_04430"
     CDS             complement(2028..3173)
                     /locus_tag="VKN64_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HMA59519.1"
                     /translation="MKESLYHIDNRCVFRETFNDEFTVRKNGRVSTNVSFKNGIANFN
                     GVSSRIVCNGILLNGTYSIRIKVKDITLSNQYLCDFRLNSGTGYIHLLQNGNFDMSSG
                     TTYSNGSLSSTYSTSTTELVISGITVDVTQISIGRRYTADFEYFFGGMDLFEIYKGAL
                     TAEEVSLLYNGKVDKELNTGTTLLDYQSTNGVIENSGKQSELTPTDVEVVKNGSFYTS
                     KFNGSSSNISIGVIDNTGNRTFTGWINPYSLGENNAGHIYSDQDYNNLYLSSGKLKLN
                     RNGSPSVAESSDIEFNKMLFFAITSTSTGVTNIYIGDKNTAPVLSGSADQEVGSPIAS
                     STVTYIGNRHAGDRAFDGLIPKLRIEEGILSLEQITQHWAETRKEVM"
     gene            complement(3170..3763)
                     /locus_tag="VKN64_04435"
     CDS             complement(3170..3763)
                     /locus_tag="VKN64_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59520.1"
                     /translation="MDKISRNMIKASYEHGLVGCIGLVKSETWLSKAIRFWMNLYRKK
                     VGKPGLKVYSHSFNIISSNGYLKVTEALAKGITESHFLDAYPKGTWNNILILKPKKPL
                     TLKEKKKFCKISRKYNDHPTRYQFTNFLSQMIWIVFGVWIGEDNKGNDKRFYCTEYSG
                     TVYNELRGYFEYKNRVNPVDLQVSDKFDHVPMQKDLV"
     gene            complement(3739..4194)
                     /locus_tag="VKN64_04440"
     CDS             complement(3739..4194)
                     /locus_tag="VKN64_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59521.1"
                     /translation="MPVILDYNNCVQIIDALVLDKGINDIIKEDFVIQGETKKIRFSI
                     LSNAIADLQEVIKAGINVMHGVSIIGYQEIEQDGETIIQVITNQVPSTLEEFKSTLLL
                     SLSVDWTQKKRNSYTFETDPVIFENIVNYVADAMVSAYSDEGWTKFQEI"
     gene            complement(4227..4958)
                     /locus_tag="VKN64_04445"
     CDS             complement(4227..4958)
                     /locus_tag="VKN64_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59522.1"
                     /translation="MKYPGLGRNVNVEQLSPYIASNYISNLNGDFTASPVIGTTKIMI
                     SGLTPHNSNVILGRFFRYDGIQVTSLDYNSFSIDGSMIDLPNIDRPFKSGDEVYCILN
                     EGRLRGFDNSLDIYKMLNQNPDKRDICSVEPVFKETNLAVGTHFKEVYPDHYELEFHT
                     SITDGTGATVKVYYSLDEDDTVPDTDGSVGGNWKEITSSVFGSAVTGTDINEIGGISS
                     KRGRFLIEVDIANATNVVKFLIKKI"
     gene            complement(4962..5090)
                     /locus_tag="VKN64_04450"
     CDS             complement(4962..5090)
                     /locus_tag="VKN64_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59523.1"
                     /translation="MECAFVNRFQEKDIEIFHTKELVPYEVAKLGLKAVNDFYNRL"
     gene            complement(5282..5665)
                     /locus_tag="VKN64_04455"
     CDS             complement(5282..5665)
                     /locus_tag="VKN64_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59524.1"
                     /translation="MSKTKEMKITIKTLIESKEALSFIFNNCALPGAISYDIMLLIDE
                     VNNHYDKHDSVKKSLMEKYGKKKGDKIVIEPSSKGYNNFIKEYEELLDKTIEIKDVKI
                     PLAVLKNQEKIKPQYFSSLAWAIKK"
     gene            complement(6186..6566)
                     /locus_tag="VKN64_04460"
     CDS             complement(6186..6566)
                     /locus_tag="VKN64_04460"
                     /inference="COORDINATES: protein motif:HMM:NF022769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMA59525.1"
                     /translation="MRYSITGELKTGIEENEIRKKDGTTFVSYEFTLKVLEKDKERFY
                     RFKVMEFVLGKIGRICKGMMVEVDFNISCREYNGRWFTNLYAWNVTQDSDFNMDEEAK
                     RVEEEDKDNDLFKTNNDIEDDLPF"
     gene            complement(6757..7203)
                     /locus_tag="VKN64_04465"
     CDS             complement(6757..7203)
                     /locus_tag="VKN64_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59526.1"
                     /translation="MIEQKLRLEADYLARAILKNKGSFNSNNIKRASEELLLSKAWLG
                     KMLSELNYVSPYKNDGKRKDVGDIEKADSLYEGDFSEFNSLSQIEKVDIIREKIKGMI
                     NDIKDIGIVKNREYNIARTNVYNHLCLTCFFLGYELERIKLKNDNK"
     gene            complement(7216..7548)
                     /locus_tag="VKN64_04470"
     CDS             complement(7216..7548)
                     /locus_tag="VKN64_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59527.1"
                     /translation="MKKDEKKLREELKMDAEVKGENSELPLQNDIQRAFFDRVAHDMG
                     DVVGKLHNNSQELASFLYALEGDLLSIDIKGTEKGDYLSVFSCLMGNLYDITKFQKEI
                     ITRLKGFI"
     gene            7826..8119
                     /locus_tag="VKN64_04475"
     CDS             7826..8119
                     /locus_tag="VKN64_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59528.1"
                     /translation="MRSKKTNFINDFEATNLEIVKPYQKKGGEIRWLRCKTPNGKNIA
                     VEEGEVVELHNTYGGVLLAGDGKELQEVKEGMECVLSTKIKFAREAGELMVLK"
     gene            8193..8699
                     /locus_tag="VKN64_04480"
     CDS             8193..8699
                     /locus_tag="VKN64_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59529.1"
                     /translation="MIINKFKTLFLKTGGEYKELPLVGEPARVQYERKKFGKIDNVKG
                     YLTGKIRTINLPPQKDNIIYIVSFEVLTENPERADLFTPFLKVKSTNSLEAYNGLFRK
                     KESTPLFTVIEDRGYGIKYEIYFGTRNECEKMAFMKNNEAMNQYHGFSCSKDMLDGNQ
                     KPYNYYVK"
     gene            8784..9068
                     /locus_tag="VKN64_04485"
     CDS             8784..9068
                     /locus_tag="VKN64_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59530.1"
                     /translation="MNNNKLTKGDEIELVFIKNEKGKRPISRLDNGKICILDRDTKGF
                     FPVGSKWVCQIIKVEDKKVIFAPLYMIATPRETALEIAEKSKSLAAKFSK"
BASE COUNT         3212 a         1916 c         1354 g         3370 t
ORIGIN      
        1 atgaaagaat tgtgatagtc tttaacttct tgttctgaga gggctctgtt gtaaatacga
       61 acatcagaga tttctccttc aaatgcacca tcaaaagttc caaaatcacc tatatttatt
      121 tcatcacttg ctgcttcaag agcatacgct gtatcagatg aatctgccaa agaaccatta
      181 acatacagtt tctgagtaga accatcaaaa gttaatatag cagtatacca tttattcgta
      241 gttaaaacag cagttgcttc tacactatca ccacttccat tttgtagtaa gtcagtaata
      301 atggcatcat tattttgtct tatatttata ccattgtttc caacagtact ttctggaatg
      361 gaaacaatat acttattagt tccacttaca ttatcaggtt taaacctcac agcaatgctt
      421 acctgtttac caccatcaaa acttggactt atactacttg ctccatagta ctcatcattt
      481 gctccactaa atttaacacc ttcttttgtc gataatggtg ttccattcct acttgcatca
      541 ttcccattac cactaatgtc aacaatcttt tgtccggaaa ttggtatcat gttgtaggct
      601 gcaactaaac cagttgtagc ttttattgaa ttgtttgtat aatatttata atttgtcata
      661 atataccacg tttaatttgt tgtcacactt acgcctttac cttcaagact tgtaacagca
      721 gcatcacttg cacttgttct agctgcgttg tttccactta tgtctattgt tccactgctc
      781 ataccactat tgtccaaatc tattaataag ttatcaacct ctgtgctgtc caatccataa
      841 ccagcagcag gaagtaataa gaaataatct attcctgaat taaaagtatg accacttgtg
      901 tatgtgctaa tagtattaga accatagcaa ataaagtatg ttagggtaga tggtaagtca
      961 gccaggtcac cactcactat gttattccca tcgcaacgaa agtatgttag ggtagatggt
     1021 aagccagcta agtcaccact cactgtgtta ttcccatagc aacgaaagtc tgttaagcta
     1081 cttggtaata tgtttaaatt aaaatcaaag tcgttatcag cactttcaaa ataagttaaa
     1141 actccatttt ttgcaaatat cttaacagta gaaggaataa cgttactctc tgtttctgaa
     1201 gatgttgaaa tctcttgaaa atcactatta cctgtccagt caacaactgc aaaatcccca
     1261 ccattgttga gtttaagact agaagtatca ctagcattat cagtaaactc cattacttgt
     1321 atgtattcat cacctgtata atgtgtgttt ctttcgtgtg acaaatcatt aggctttgat
     1381 aaaacgtatt ttgttgaagc gttaccctta gtaataggag agcttcttaa gaagtcattg
     1441 tatgaattat gaatatctgt taaagaaaaa ggattgttat gcaatacaca ctttactata
     1501 taaccattat gataataaga tgatctttca gtaccaacat ataaagtaca aacttctgtt
     1561 ggatagctta ttgaaggagt tccagaacca tcaaaagtac cgtttaaata tatactaaca
     1621 ataccacctt cattaacaat aataatatga taataagtac caattttcat agatgtagat
     1681 cctataaaag aatcaccccc tcttgaaaaa aactgtattt tatcccctcc aaattctgta
     1741 tttaaagcaa tactattatc accaccttga tataatgata atattgtttt tgtatcgata
     1801 gaaacattta attcattaaa ttttacaata gcttcgattg taaaatcacc agatgttaac
     1861 tcaatttcag aatattctat attagtacta cttccatcaa agttcatagc caatcctttt
     1921 tcggtctttt taaaaccacc atcacctaca gtcagaactc ctatactgtt atctacttca
     1981 ttaataagac taccctttcg tgcatcaaat tcaaaaatct tgcccattta cataacctcc
     2041 ttccgtgtct ctgcccaatg ttgcgttatc tgttcaagac taagtattcc ttcttcaatt
     2101 cttaatttag gtattaaacc atcgaaagcc ctgtccccag catgacgatt accaatataa
     2161 gtaacagttg aactagcaat aggagatcct acttcttgat cagcactacc gcttaataca
     2221 ggggcagtat ttttgtcccc aatataaata ttggttaccc cggtacttgt tgatgttatt
     2281 gcaaaaaaca acattttgtt aaattcaata tcactactct ctgcaactga cggactacca
     2341 tttctattta attttaattt accacttgat agataaagat tgttgtaatc ttggtcacta
     2401 taaatatgcc cagcgttatt ttctcccaaa ctataagggt taatccaacc cgtaaatgtc
     2461 ctattaccag tattatctat aacaccaatg cttatattac tagaactacc gttgaacttt
     2521 gaagtataaa aactaccgtt tttaacaacc tcaacatcag taggggtaag ttcagattgt
     2581 ttgccactgt tctcaattac cccgttagtt gattggtaat caagaagagt tgttccagta
     2641 tttaactctt tatcaacctt accattatat aacaaactaa cctcttcagc agttaaagca
     2701 cctttataaa tttcaaataa atccataccg ccaaaaaaat attcaaaatc ggctgtatac
     2761 cttctgccaa tgcttatttg tgttacatct acagtaatac cagaaattac aagttccgtt
     2821 gtagaagttg aataagttga agataatgac ccattagaat aagtagttcc tgaactcata
     2881 tcaaaattac cattttgaag taaatgaata tatcctgttc cagaattaag cctaaaatca
     2941 caaagatatt ggttagataa tgtaatatct tttactttaa ttctaatact ataagttcca
     3001 tttaacaata ttccattaca aacaattcgg cttgaaaccc cattaaaatt agcaattcca
     3061 ttcttaaaag aaacattagt ggatacacga ccgtttttac ggacagtaaa ctcatcatta
     3121 aaagtttccc tgaaaacaca cctgttatct atatggtata aagattcttt catacaagat
     3181 ctttttgcat tggcacatga tcaaacttat ctgaaacttg taaatcaacc gggttaacac
     3241 ggttcttgta ttcaaaataa ccccttaact cgttataaac agtaccagaa tattcagtgc
     3301 aataaaacct tttgtcattg cccttgttat cttcccctat ccaaacgcca aaaacaatcc
     3361 atatcatttg ggaaagaaaa ttggtaaatt gatatcgggt agggtgatca ttatatttcc
     3421 tggatatctt acagaatttt ttcttctctt ttaatgtaag gggtttttta ggttttaata
     3481 tcagtatatt gttccaggta ccttttggat aagcatctaa aaaatgactt tcggtaatac
     3541 cttttgctaa agcttccgtt actttcaaat aaccattgct tgaaataata ttaaaagaat
     3601 ggctatacac tttcaaccct ggctttccaa ccttttttcg atataagttc atccagaacc
     3661 ttattgcttt agaaagccac gtttcactct ttacaaggcc tatacaacct actaaaccat
     3721 gttcataact tgccttgatc atatttcttg aaattttgtc caaccttcat ctgaatatgc
     3781 tgaaaccatg gcatctgcta cgtaatttac aatattttca aatatcaccg gatctgtctc
     3841 gaaagtataa ctattccttt tcttttgtgt ccaatctaca gataaagaca acaacaaagt
     3901 cgatttaaat tcttctaacg tagagggtac ctggttcgta ataacctgaa tgatagtttc
     3961 cccatcttgt tcaatctctt ggtaaccaat tattgaaacc ccatgcataa cgtttatccc
     4021 ggccttgata acttcttgaa ggtctgcaat ggcatttgaa agtatagaaa acctaatctt
     4081 cttggtttca ccttgtataa caaaatcttc tttgataata tcatttatac ctttatctaa
     4141 aacaagggca tctattattt gtacacaatt gttataatct aatataaccg gcactgtgct
     4201 tatacttggt tttgcttttg gaaatatcat atttttttaa ttaagaattt aacaacatta
     4261 gtagcattcg ctatgtctac ttctatcaaa aaacgccccc ttttactaga tattccgcct
     4321 atctcgttaa tatctgtacc tgtaacggca ctaccaaaaa cacttgaggt aatttctttc
     4381 caattgccac caacagaacc atcagtgtcc gggacagtat cgtcctcgtc caaactgtaa
     4441 taaaccttta cagtggcacc agtgccgtca gtaatagaag tgtggaactc tagttcataa
     4501 tgatcagggt aaacctcttt aaaatgtgta ccaacagcaa gattcgtctc tttaaacacg
     4561 ggctcgacac tacatatatc ccttttatcc gggttttggt taagcatctt atagatatca
     4621 aggctgttat caaaacccct taacctcccc tcgtttaata tgcaataaac ctcgtccccc
     4681 gatttaaaag gcctatcaat gtttggcaaa tcaatcatac tgccatctat tgaaaaagaa
     4741 ttataatcaa gcgaggttac ttgaatacca tcataacgaa aaaaacgccc gaggataaca
     4801 ttactgttat ggggcgttaa tccacttatc attatttttg tcgtgccaat tactggtgaa
     4861 gctgtaaaat caccatttaa atttgatata taattactgg caatataagg ggataattgt
     4921 tcaacgttta cattcctacc taaacctggg tatttcatac tttataatct gttgtaaaaa
     4981 tcattaacgg ctttcaaacc aagcttggca acttcgtaag gtaccaattc cttggtatga
     5041 aatatctcaa tgtctttttc ttggaaacgg ttgacgaaag cacattccac accttctata
     5101 aaaatccttt tctgatagta ttttccgttt ttcttgtagt ctgaatttac tttgtcggtc
     5161 tctatttcta cagacctttt ctttatgctc ttgacagtat attgaaaatt aaggtttgtc
     5221 ctatgtacaa caggattgcc tatttttatc caacattttg gatatgaatt tattatatct
     5281 tttatttctt gatcgcccat gccaaagaac taaagtattg cggtttaatt ttttcttggt
     5341 tctttaaaac tgcaagaggg atttttacat cctttatctc gatagtttta tccagcaatt
     5401 cttcatattc ttttataaaa ttattataac ccttgctgct tggttcaatt actattttgt
     5461 cgcctttttt cttgccatat ttctccatta ggcttttttt gaccgaatca tgcttatcat
     5521 aatgattgtt gacttcatca atgagcaaca tgatatcata cgaaatagcc ccaggaaggg
     5581 cacaattatt aaaaataaat gacaaggctt ctttagactc tataagggtt tttatggtta
     5641 ttttcatttc ctttgtttta gacaaaaata ataattttat tcaataatac aaatatcctc
     5701 ccctttctag tcaagatatt aatattgaag ggaaaacctt ttaatatctt atactagttt
     5761 atatcaagat aataataata cttgatatct gtcttttctt ttccgatccc atctagtaag
     5821 gagggtattt attttttttt atcttgactt accagttgtc gggagtagga cagtgctgga
     5881 ttatgatacc ctagaaaaaa aagactgtag gtttttttat gtcctgatag acaaaggata
     5941 aaactcttgg tcctcataac ccttggaata caaatataaa aaaaatcccg aaagaacaat
     6001 gccttccggg atttattact cccttcccct ttaggttatg agggccatta ggggcctaag
     6061 aatgttgcaa atataactaa tttaaaccaa ataaaaaaac atctaactct tcatttcgtt
     6121 cgttataaat tatcatacca acaaaccctg tgttttttat taagaaattt ttcttttctt
     6181 caatactaga atggtaaatc atcttcaatg tcattattag ttttaaacaa atcattatct
     6241 ttatcttctt cttctaccct ttttgcttct tcgtccatgt taaaatcact atcctgggta
     6301 acgttccaag catataggtt agtaaaccat ctaccattat attcccggca agaaatatta
     6361 aaatctacct ctaccatcat ccccttacat atccggccaa tcttcccaag aacaaattcc
     6421 attaccttaa aacgataaaa cctctcttta tctttttcca atacttttaa cgtaaattca
     6481 taagaaacaa aagttgtacc gtcttttttt cgtatttcat tttcttcaat acctgttttt
     6541 aattctcctg ttattgaata cctcatacaa catgtaatta atatttagaa ttatattcat
     6601 ctattgcctt atctatggct tcctttaaat aagcggacat atcacctacc gccctcttga
     6661 acttgtcttt tttgtccaaa cttatccgaa attttataaa tccgctttcc tctttttcct
     6721 tcggctttaa ttttactttc atatattata tataaattat ttgttatcat ttttaagttt
     6781 tattctttct aattcataac caaggaaaaa acaggtaaga cataaatgat tataaacatt
     6841 cgtcctggct atgttatatt ccctgttttt tacgatacct atatccttaa tatcattaat
     6901 catccctttt atcttttccc taataatatc taccttctca atttgtgata aactattaaa
     6961 ctcagaaaaa tcaccctcgt aaagactatc ggccttttca atatcgccca catccttcct
     7021 tttcccatca ttcttatacg gggacacata atttaattcc gataacatct tgccaagcca
     7081 agcttttgac aataataact cttctgaagc cctctttata ttattgctgt taaaagaacc
     7141 cttgttcttc aatatagcac gtgccaaata atcagcctct aaacgtaatt tttgttcaat
     7201 catgataatc cttttttaaa taaaaccttt taacctagtt attatctctt tctgaaattt
     7261 tgttatgtca tataaattgc ccatcaaaca agaaaaaaca ctcaaataat cccccttttc
     7321 agtacccttt atatctatag aaagcaaatc cccttctaat gcatataaaa aagaagctag
     7381 ttcctgtgaa ttgttgtgca acttccctac aacatccccc atatcatggg ccaccctgtc
     7441 aaaaaatgcc ctctgaatat cattttgcaa gggcaattca ctattttccc ctttaacctc
     7501 agcatccatt ttaagttctt ctcttaattt cttttcatct tttttcataa caaatcattt
     7561 taaatgttta atactcaatt taacaatccc tacaaatgta attatttatt tgtacccaca
     7621 caaggttttt tcaaaaaaaa tgttcctaca atgccccaag gtaaaccccg aaaatttatc
     7681 ccccctgtgc ccacacctgt tttcgctata tagtaataaa tagggggggt accccccttc
     7741 aaccctcccc acctcttgca gaatcaaaat tttcttattg tttaacaaaa aattattaat
     7801 tatgaagaca tttgaaatcc aaaacttgag gtcaaaaaag acaaatttta ttaatgattt
     7861 tgaggcaaca aacctcgaaa ttgtaaaacc ctatcagaaa aaaggcggag aaatccgttg
     7921 gttacgatgt aaaactccaa acggcaagaa tattgctgtt gaggaaggtg aagtagtaga
     7981 actacacaac acctatggtg gtgttctatt agccggtgac ggcaaggaat tacaagaagt
     8041 aaaagaaggc atggaatgtg tgttaagcac taaaattaaa ttcgctcgtg aagccggaga
     8101 actgatggta ttgaagtaaa aggagggggg gcatcccctc ttttttttta actttgtctc
     8161 ccccccacag gggatatata acaagataaa aaatgataat aaacaaattt aaaactctct
     8221 tcctaaaaac aggaggggag tataaagagc ttcctctcgt gggggaacct gccagggttc
     8281 aatacgagag gaagaaattc ggcaagatag ataacgtaaa aggttatctt accggaaaga
     8341 ttaggacaat taatttgcca cctcaaaagg ataatattat ttatattgtc tcttttgagg
     8401 tattgacaga aaaccctgaa agggcagatc ttttcacgcc ctttttaaag gtaaaatcta
     8461 caaattcctt agaggcttat aatggcctct tccgtaaaaa ggaatctaca cccttgttca
     8521 ctgtaataga ggacagggga tatggtatca agtatgaaat atattttggt accaggaatg
     8581 aatgtgaaaa aatggctttc atgaaaaaca atgaggccat gaaccaatat catggttttt
     8641 catgttcaaa ggatatgtta gatggtaacc aaaagccata taactattat gtaaaataat
     8701 aaaataaata ccatgaaaaa aattgattta ttaaaaatca tagtttttct tattataata
     8761 ggaatgactt taataacaca aatatgaaca acaataaact gaccaaagga gacgagatag
     8821 aactcgtttt cataaaaaat gaaaagggca aacgccctat aagccgtctt gataacggca
     8881 aaatttgtat actcgacagg gatacaaaag gatttttccc tgtagggtca aaatgggtat
     8941 gccaaataat aaaggtggaa gataagaagg ttatctttgc ccctttatac atgatagcta
     9001 ccccaagaga aacagcattg gaaatagccg aaaaatcaaa gagcttagcg gctaaattct
     9061 ctaagtaaca cttcaaggga aagcataatc cctatgcttt cccttgcttt taatttgtta
     9121 ctttaaaccc cctttttttg ataaactatg aaatagagag tacttacaat ctgtaagtat
     9181 attattatgg aaaaaatcct tagaagcctt atcccactag gctttatcta caatattgac
     9241 tataatggca tcatcgtatc aaaaagtatc tataaagatt tggtatttgt gataaccatt
     9301 acaaaagaaa acccttattg ccttcagctt acatgttatc catcaattgc ctcaaaaaga
     9361 aagcatcaca aggtgcctga cttaactttt gacataatta actataacct ggtagaagta
     9421 tacaaagaag tggaaaaatg tatgttcaaa tatcttgcat ataacaattc ccttatgtta
     9481 tacgacaagg aaaacccttt atgacgcacc agcaggggcg tagagacacc cccctgtccc
     9541 ctcttcttca tcctcatgtt ttacttgttg ctattattgc ttatagttat tgtttatagt
     9601 gttagatatt atatggggat aagtttttat taattatttg ataaggtctt gtattttgtt
     9661 tttgctttat taaggttatt tttatagctt tatttagtta ttttggtaat ttatgctatt
     9721 aatatttttt atttgaatat tatccttaaa taaactgtaa taatgatatt ttgtataatc
     9781 caaaggtgga atagtaaaca aaacttctac taaataccca gaagggtatc aagtataaaa
     9841 tataaagtgc ca
//
LOCUS       DATGCJ010000086        13671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_56175, whole genome shotgun sequence.
ACCESSION   DATGCJ010000086 DATGCJ010000000
VERSION     DATGCJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13671
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56175"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VKN64_04490"
     CDS             complement(<1..215)
                     /locus_tag="VKN64_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HMA59531.1"
                     /translation="MKAKQIAGAKAAEYVKDGMIVGLGSGSTAAWATREIGARVKDGL
                     NIRAIPTSEETRQLAEELDIPLTTLSEV"
     gene            complement(302..2311)
                     /locus_tag="VKN64_04495"
     CDS             complement(302..2311)
                     /locus_tag="VKN64_04495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMA59532.1"
                     /translation="MKKKITVLAIDEQVSKFFKSELEKIFSNLFIIDYRYPDMEFVPP
                     ITDTDLILYTDPEILNIMVDKIRCDAPTLMMKRTITKEALNKIREIPAGKKVLVSNIN
                     QYMANETMALLYQLGITDVKFYPYYEGKSEYPESVEYIIVPEDEPYEFLPDIEAEIIL
                     IGNRVFDISNVLDILSILRIDSVKAENIIKKYLIKVPTIWYGFDYGWENRRVLLNQWE
                     LLLDELMSGVIVTDETNQIELINYKAKNILKLEKENVVGKDINILINKVNNLDFILEE
                     KEVKDKLVKFKGKELIFTVKSVEFDDQFYGKIIIIAPYREMVKTQQKVHKQLVGKGHY
                     TEHTFQDIIGDSTLIKKAKEISRKIARTDSTVLLLGDSGTGKELFAGAIHNYSHRSRK
                     PYVAINCATLPENLLESELFGYEEGAFTGARKGGKIGLFEQAAGGTIFLDEIGDLPLN
                     LQARLLRAIEEKEIMRLGGDSIINIDTRIIAATNQDLFQLMEEGKFRKDLFYRLNVFQ
                     IKIPSLTERLEDIPTLIDYYLEQWGEYKEITSDLEEFIKKYEWPGNVRELKNTIKYMV
                     TVGEEQLSFANLPDYLKTADNSPKKDALLLKRLYHLAQDGNDTGRRSLTEAFKHNYYE
                     ISEMEVRKILNNLKENDMIKVARGRAGTSITESGLDYLNNKGYIS"
     gene            2521..3945
                     /gene="glnA"
                     /locus_tag="VKN64_04500"
     CDS             2521..3945
                     /gene="glnA"
                     /locus_tag="VKN64_04500"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HMA59533.1"
                     /translation="MFNSFEELKRYVEKNNVKTLDFKIPELKGSWKHLSVPAQRLKPE
                     IFEEGFGFDGSNYGYANVEKSDMVFKPDYRTAFEDPFWEEKTLAFLGRIYEVGKDGDI
                     PFNGESRNVLLRSLEYMRENDYADQFILGPEFEYYIFDEVYYWNQNHNTGFNVYSQQA
                     AWDRDNYDGFKVNPKRGYHVDAPHDRDKDLRAAISTALEDLGIEVKYHHHEVGAPGQH
                     EIETERVPAEKMADDTMKIKYFIKNMAVQWGKTATFMPKPLYGEAGNGFHVHMQLLKE
                     GETIFSSQESEYAGLSKKALYFMGGVLKHTPALMGLAAPSTNSYRRLVNGYEAPVAIC
                     FSKANRSAVIRIPGYATDPSDRRFEFRPGDASGNSYLTYAALLMAGLDGIENEIDPIE
                     EGYGPIDKNIYDLTEKEKADLEFLPKDLEEAIDSLENDHDFLLKGDVFSEKLLEAWLE
                     SKKHECQYIYNRPTPDEYKLYFDV"
     gene            complement(4005..4646)
                     /locus_tag="VKN64_04505"
     CDS             complement(4005..4646)
                     /locus_tag="VKN64_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA59534.1"
                     /translation="MKIKWWGHATFTITTFQGTKIVTDPYNEDLPYKIVNDKADIVTV
                     SHDHFDHNAVDLVPGSPSIVRDISGYSDKEIKIKGIKSYHDDQSGNERGENIIFIYNF
                     NDIKLSHLGDLGHPLNEEQKEALKDVDVLLIPVGGHFTIDADQAYEIVKQINPKVIFP
                     MHYKTDILDLPIKGVDKFLKHFDHDSIVRVNGPEIEFSSLPFEQKVYVPEYVK"
     gene            complement(4761..5204)
                     /locus_tag="VKN64_04510"
     CDS             complement(4761..5204)
                     /locus_tag="VKN64_04510"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMA59535.1"
                     /translation="MAKSSAETVKEYLNELPEGKREVVKKLRQIVSDNLPNGYQERMN
                     WGMISYEVPLTKYPSTYNKKPLLFAAISAQKNYFSLYLTPVYQSKELEKELKREYKKA
                     DLKVNMGKSCLRFKKLSELPLEFIGELISRIEVEDFIRIYEESRK"
     gene            complement(5220..5774)
                     /locus_tag="VKN64_04515"
     CDS             complement(5220..5774)
                     /locus_tag="VKN64_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59536.1"
                     /translation="MRIIKKYRIYLFLFIASLLTVIIHEYGHFLVGTVLGYNMGFDFN
                     GSRPLIGTYANNTHYFLVIIGGISFTIVQSYLAFYLMEKLNYKDLYYLVLTPFLYRLI
                     PYLISVIYPNRLLLQDEARVGNLLGINLYIIPIIIMFVLLFNCYRANKKYKISFKKHI
                     KLSFISIISFIIIIRLNQLLLVSV"
     gene            complement(5897..6265)
                     /locus_tag="VKN64_04520"
     CDS             complement(5897..6265)
                     /locus_tag="VKN64_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA59537.1"
                     /translation="MEFLFNTIEVADLDQSYEFYSEILKLEVVRRIEPEEGVKIIFLE
                     DGEENTVELIERSGESKADKAKESKIKMGFGVEDLDQTIRFLQEKGIEIKEGPVQIKG
                     GSFINIEDPDGVMIGLYEFK"
     gene            complement(6284..6868)
                     /locus_tag="VKN64_04525"
     CDS             complement(6284..6868)
                     /locus_tag="VKN64_04525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA59538.1"
                     /translation="MADIDQDKIFFTDKQIVDIEEKNNMGRILDIGGGGEAVMAQVYG
                     DNVVAIDIDEEELLESPENQALKIVMDARNLYFLENQFEMVTSFFTLMYLENNDIGQV
                     FEEVKRVLKPNRSFLIWDLTIPARQSHKEEVYAAQVEVNFNENTINTGYGARWQDNEQ
                     SMETFINLAKDYNFSVLEKRQGDKFFFVELRNNK"
     gene            complement(6861..7160)
                     /locus_tag="VKN64_04530"
     CDS             complement(6861..7160)
                     /locus_tag="VKN64_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMA59539.1"
                     /translation="MKNNIFVRIDRRVSEGIIEEPVFNLHLKYIEELKDNGFLIAGGF
                     EQKDGGMVIFTAASEAEAEKISRDDPIIKKGYYDFDLYNWDIKYISNIEEVLASG"
     gene            complement(7175..8080)
                     /locus_tag="VKN64_04535"
     CDS             complement(7175..8080)
                     /locus_tag="VKN64_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA59540.1"
                     /translation="MNKTKGILLTIFAAVGFGFQGFLAKTAYANGFNPYSFTLYRSLF
                     AAVALFIFIKLYNLDMEVTKAQYKTLFKVSLFGYSIMLVILYISFLYMPTGLAMTLHF
                     VYPVFVMLSSVFLYKEKIGPKKVLALILSISGIYFLMGFGSFSNISMLGFWLALISGI
                     LYSYYILMVSNSNIKDLNSFVITFYLSLFNTFIVFALTLVTGNISLNYNYKGLLSTIM
                     VALICNIFGMVSFQAGLKYITATTSSILSTFEPITSLIVGILVFNEFLAWYHFAGSIL
                     IIVSVILVSLAEKRLAEVADKSKIS"
     gene            complement(8123..8671)
                     /locus_tag="VKN64_04540"
     CDS             complement(8123..8671)
                     /locus_tag="VKN64_04540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMA59541.1"
                     /translation="MNLYFIRHAETIYRSEDEDFNRPLSTKGKKDAEKLVNIFKDISI
                     DKVYASPYFRAIDTVKPLAEDNELSIELMDNLRERKVADHYIDDFFEFAAKQWNDFDY
                     SLPGGESFNEVQERGLKVLNKIVNNIKQGNIIVAGHGTWLGVILNYFDDSFDYEHWRN
                     IRMPDIYLFKYLNRNLKEIKKI"
     gene            complement(8668..9207)
                     /locus_tag="VKN64_04545"
     CDS             complement(8668..9207)
                     /locus_tag="VKN64_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMA59542.1"
                     /translation="MQLNKIIYKKDNLDFPPKTFEREAVRGVILKDNELLMLYSQKYG
                     DYAFPGGGVEKGETRFQALKREIIEECGIIISTDIDSLGKIIEYNKAPVEGYDILKMT
                     SYYYLTDIKEKLGYSDLEDYELELELETRWVDINKAIANNIEIMESYKETPFWALREV
                     KALQYIKDNIIIDREGEDN"
     gene            complement(9232..9762)
                     /locus_tag="VKN64_04550"
     CDS             complement(9232..9762)
                     /locus_tag="VKN64_04550"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA59543.1"
                     /translation="MKEVGLIDKISFNLMAAEYKIKSIFSPPEEILKQTNIQPGDHVV
                     DYGCGPGRFTIPLAQMVGNKGKVFAVDINPIAFEKVKNKAKTENLENVEYIDGHNIKM
                     IEKESIDVVILYDTLHDLNDMRGTVKSISGMLKTGGYLSFKDHTLKKQCIKEMTANLP
                     LNLERENEKTIEFKKV"
     gene            complement(9845..10117)
                     /locus_tag="VKN64_04555"
     CDS             complement(9845..10117)
                     /locus_tag="VKN64_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59544.1"
                     /translation="MFYNFWALVTVVLALLISYYTFRNDIQQKIISFAQLLVWTVSLI
                     SLVIGLSNLFEIKVLGSLVSFVILANLYMAVVNIGVRLQKRISRNI"
     gene            complement(10299..11102)
                     /gene="proC"
                     /locus_tag="VKN64_04560"
     CDS             complement(10299..11102)
                     /gene="proC"
                     /locus_tag="VKN64_04560"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMA59545.1"
                     /translation="MKLCIIGLGKIGTALMQGILNSGLYEADELIGCDIKAKETKNNE
                     DYGGIETTDDNVYGVEQADAVLLAIKPQVIDHVLEEISEAAKGKLIISIAAGISNSHL
                     KEKLPLDVRTIRVMPNTPVLVKSGISAIAPGRNAAEEDIKLVNRIFAGVGEVVEVEEN
                     LMDAITGLSGSGPAFIYIMIEALADGGVLTGISREMALKLTAQTMLGSAKMVLETENH
                     PGVLKDMVTSPGGTSIRGIEVLEKHGVRGILIDAVKEAALRSKELNTLD"
     gene            complement(11111..12367)
                     /locus_tag="VKN64_04565"
     CDS             complement(11111..12367)
                     /locus_tag="VKN64_04565"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HMA59546.1"
                     /translation="MSIREEVVSQAKLAEEASLKTANISTEVKNKALYNIAEGLMNKQ
                     SEILAANNKDMEKAREQNLSKAFLDRLSLDEDRIKGMADGLMEVAELDDPIGDILEMK
                     KRPNGLQIGKMRVPLGVIGIIYEARPNVTVDTAALCLKSGNTVLLRGGSNAFNSNKIL
                     TEIIQANAHEAGMPAGTVQLIQTTDRAAVKELLTLNDYLDLLIPRGGAELINRVINES
                     KIPVIQTGVGNCHIFVDETADIKKAAEIIVNAKTSRPAVCNAAESLLIHRELANDFIP
                     RIAKVLSEYDVEIRGDDTAVKIENSIKKADESDWGKEYLDYIISLKVVDNIDHAVENI
                     NKYGTKHSEAIITEDYTNAQQFLNEVDAAAVYINASTRFTDGNQFGLGAEIGISTQKM
                     HARGPMGLNELTSTKYVIYGQGQIRE"
     gene            complement(12385..13218)
                     /gene="proB"
                     /locus_tag="VKN64_04570"
     CDS             complement(12385..13218)
                     /gene="proB"
                     /locus_tag="VKN64_04570"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HMA59547.1"
                     /translation="MQENDFKRVVVKIGTSCLTHDNGKLNYTAIEKYLRQLVDLKNMG
                     KEVLLVSSGAIGAGIGELKLNLPCSMAEKQGMAAVGQGLLISLYNKILRQYGELGGQV
                     LLTSSDLKEKERAKNIYNTLEVLLKNDIIPIINENDTVATDEIRFGDNDTLSARVSRL
                     IKADLLILLTDVEGMYNKIPCDTEEDLELINKVENITPDIEEKAGDEGSIVGTGGMKT
                     KLEAAKIVNEAGITMVIGPAYRDNVVSKIIEMVKEDSEYKIGTTFLPNDKQCKRKISN
                     H"
     misc_binding    complement(13284..13529)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4180 a         2732 c         1964 g         4795 t
ORIGIN      
        1 acctcagata aagtagttaa aggaatatct aattcttctg ccaactgtct ggtctcttct
       61 gaagtaggta tagccctgat atttaaacca tcttttaccc tggctccgat ctctctagtt
      121 gcccaggctg ctgtagaacc acttcctaaa cctacgatca ttccatcctt aacatattcc
      181 gctgcctttg ctcctgcaat ctgtttagct ttcatccttt aatcccccta ttattaattg
      241 aaatagatat ttttttcttt atattaatta tatacctttt taacattaat ttctttaaaa
      301 attaagaaat atatcccttg ttatttaaat aatctaaacc gctctcagta attgatgtac
      361 cggctctacc ccttgctact tttatcatat cattttcttt taaattattt aaaatttttc
      421 taacttccat ctctgaaatt tcataataat tatgtttgaa agcttctgtt aaactccttc
      481 tacccgtgtc attaccatct tgagctagat ggtaaagcct tttcagtaat aatgcatctt
      541 ttttaggaga gttatcggct gtttttaaat aatccggtaa gttagcaaaa gacaattgct
      601 cttccccaac ggtaaccatg tattttatgg tgttttttaa ttccctaaca ttaccgggcc
      661 attcgtattt tttaataaat tcttctaaat cacttgttat ttctttatat tcaccccact
      721 gttctaaata gtagtctatt aaggtaggta tatcttctaa cctttctgtt aaagaaggta
      781 ttttaatctg aaagacatta agcctgtaaa aaagatcttt tctaaacttt ccttcttcca
      841 tcaattgaaa taggtcttga ttggtagccg cgataatgcg ggtatcaata tttatgatcg
      901 aatctccacc caatcgcatg atctcctttt cttcgatagc cctcaataag cgggcctgta
      961 aatttagtgg taaatcccct atttcatcta aaaagatagt acccccggcg gcctgttcga
     1021 aaaggccaat cttaccacct ttgcgggctc cggtaaaagc tccttcttca tatccaaata
     1081 gctctgattc caagaggttt tccggtaaag tagcacagtt tatagctaca tagggttttc
     1141 ggctacggtg cgaataatta tgaatagcac cagcaaatag ttctttgccc gtaccgctat
     1201 cacctaaaag taatacagtt gaatcagtcc gggctatctt tctacttatc tctttggctt
     1261 ttttaatcaa agtactgtcg cctatgatat cttgaaaggt atgttcggta tagtggcctt
     1321 taccgactaa ctgtttatgt actttttgtt gggttttaac catctcccgg tagggagcaa
     1381 ttataattat cttgccgtaa aactgatcat caaattctac agatttaact gtaaaaatta
     1441 attctttccc tttaaactta accaacttgt ccttcacttc tttttcttct aagatgaagt
     1501 ccaaattatt tactttattt atcaaaatat ttatatcttt accgaccaca ttttcttttt
     1561 ctagttttaa aatatttttt gccttataat taattaactc tatctgattg gtttcatcgg
     1621 tcactattac accgctcatt aattcatcta aaagcagttc ccactgattt aaaaggaccc
     1681 gccggttttc ccagccgtaa tcgaatccat accagattgt gggtaccttt attaaatatt
     1741 tttttataat attctcagcc ttaacactgt cgattctcaa aatagatagg atatccaaca
     1801 cattggaaat atcaaagact ctattgccga ttaatattat ttccgcctcg atatcgggga
     1861 gaaattcata gggttcatct tccggaacga tgatatattc cactgattca ggatattcac
     1921 ttttcccttc atagtatggg taaaatttaa catcggtgat gcctagttga tataataagg
     1981 ccatagtctc attggccata tattggttta tattactaac caatactttc ttgccggccg
     2041 gtatttctct tatcttattt agtgcttctt ttgttatcgt cctcttcatc attaaagtag
     2101 gtgcatcaca tctaatttta tcgaccatta tattcaatat ttcagggtct gtatagagta
     2161 ttaaatctgt gtctgtaata ggtggaacaa attccatgtc aggataccta taatcaataa
     2221 tgaaaagatt agaaaaaatc ttctccaatt cacttttaaa aaatttagat acctgttcat
     2281 cgattgctaa tactgtaatt ttctttttca taaatatcac cttttcaatt taataatggg
     2341 atagataata attattattt taatatatcc cattcaacta tattatatat taaacatgag
     2401 acctcatatt cccttttaat aaggctttct ttttatttta aatatggcat ataatatgca
     2461 ttatacataa ttgaataatg tctggaatta ttttattatt tttaacaggg ggttaaatta
     2521 atgtttaata gctttgaaga acttaaaaga tatgtagaaa aaaataatgt gaaaacccta
     2581 gattttaaaa taccggaact aaaaggaagt tggaaacatc tctcagttcc cgcccaaaga
     2641 ttaaaacccg aaatttttga agaaggattt ggttttgacg gttcaaatta tggatatgcc
     2701 aatgtggaaa aaagcgacat ggtctttaaa ccggactaca gaaccgcttt tgaagatcct
     2761 ttctgggaag aaaaaacact cgcttttctg ggcaggatct atgaagttgg taaagacggt
     2821 gatatcccat ttaacgggga atcacgcaat gtattattaa ggtcattaga gtatatgaga
     2881 gaaaatgatt atgctgacca attcatatta ggacctgaat ttgaatacta tatcttcgat
     2941 gaagtatact actggaacca aaaccataat actggtttta atgtctactc ccagcaggca
     3001 gcctgggacc gtgataatta tgatgggttt aaggtcaacc ccaaaagggg ttaccatgtg
     3061 gatgctcccc atgataggga caaagattta agagctgcta tctccacagc cttagaagat
     3121 ctcggtattg aggttaagta ccaccatcat gaagtaggtg cacccggtca gcatgaaatt
     3181 gaaacggaaa gagtaccggc tgaaaagatg gctgatgaca ccatgaaaat taagtacttt
     3241 attaaaaaca tggcagtaca gtggggtaaa acagctacct ttatgcccaa acctttatat
     3301 ggggaagcag gcaacggatt tcatgttcac atgcagttat taaaagaagg ggagaccatc
     3361 ttcagcagcc aggaaagtga atatgccggc ttatctaaaa aagcacttta tttcatgggc
     3421 ggagtattaa aacatacacc agccttgatg ggtttagccg ctccctcaac taactcttac
     3481 cgcagactag ttaatggtta tgaagcaccg gttgcaatct gtttttctaa agcaaaccgt
     3541 tcggctgtaa tcaggattcc gggctatgca actgatcctt ccgaccgaag attcgaattc
     3601 aggcccggtg atgcctcagg taattcatat ctcacctatg cggccctctt aatggcgggc
     3661 ttagatggaa ttgaaaatga aattgatcct attgaagaag gttacggccc gatcgataaa
     3721 aatatttatg acctaaccga aaaagaaaaa gctgacctgg aatttttacc aaaagacctg
     3781 gaggaagcaa ttgattctct agaaaatgac cacgacttct tgcttaaagg ggatgtcttt
     3841 tcggaaaaac tgcttgaagc atggttagaa agtaaaaagc acgaatgtca atatatttat
     3901 aataggccaa ctcccgatga atacaaatta tactttgatg tttaaagaaa ggaagggttc
     3961 cgaaaaatga ttcggaaccc tttttatact ttattcttat ttaattattt tacatactct
     4021 ggaacataaa ctttctgttc aaaaggtagt gaactaaact caatctctgg tccattcact
     4081 cgcacaatgc tgtcgtgatc aaaatgcttt aagaacttat ctacaccttt tattggtaga
     4141 tcaaggatat cagttttata atgcatcgga aaaatcactt tcgggttaat ttgctttact
     4201 atctcatagg cctggtcagc atcaattgta aaatgtccgc ccaccggaat taaaagtaca
     4261 tcaacatctt ttaaagcttc cttttgttct tcatttagag gatgacctaa gtctcctaaa
     4321 tgacttaatt tgatatcatt gaaattatag ataaatatta tattttcgcc tcgttcgttg
     4381 cctgactggt catcatggta actttttatt cctttaatct ttatttcctt atcactataa
     4441 cctgaaatat ccctaactat agaaggactg ccgggcacta aatctacagc gttgtgatca
     4501 aaatgatcat ggctgacagt cacaatatcg gctttatcat taactatctt atatggtaaa
     4561 tcttcgttat agggatctgt tacaatttta gtcccttgga aagttgtgat agtaaaagtc
     4621 gcgtgacccc accatttaat cttcactttt atctacctcc ttatctattg aaatattcaa
     4681 cttgaatctg tacaaattca gtctgaaatt taatttatgg acttcatata atttattcaa
     4741 taaaaaagaa tatatcctcc ttatttcctg ctctcttcat atatcctaat aaaatcttcc
     4801 acctcaattc tacttattaa ttcaccaata aattctagtg gaagctcaga gagtttctta
     4861 aaccgcagac aggattttcc catattaact ttcaaatcag cctttttata ctcccttttt
     4921 aattcttttt ctaattcttt agattgatat actggtgtta aatagagtga aaagtagttt
     4981 ttctgggcag aaatggctgc aaataataaa ggttttttat tataagtaga gggatatttt
     5041 gtcagaggaa cctcataact aatcatcccc caattcatcc tttcctgata tccatttggt
     5101 aagttatccg atacaatttg tcttaacttt tttactacct ctctttttcc ttccggtaat
     5161 tcatttaaat attcttttac agtttcagca ctgcttttgg ccatcatttc acctcttctc
     5221 tatacactta ccaacagcag ctggtttaag cgaattataa tgataaaaga aatgatactt
     5281 ataaaagata gtttgatgtg ctttttaaaa ctaattttat actttttatt ggctcggtag
     5341 cagttaaata ataatacaaa cattattata ataggaatga tatagaggtt aatccccaat
     5401 aagttaccta ctctagcctc atcctgaagg agtaatctat ttggatagat aacacttatt
     5461 aaataaggta tcagtctata tagaaaggga gttaagacaa gataatataa gtccttataa
     5521 tttaattttt ccattaaata aaaggctaga taagattgaa ctattgtaaa agaaattccg
     5581 ccaataatga ccagaaaata atgagtatta ttggcatagg tcccaattaa aggccgtgaa
     5641 ccgttaaaat cgaaccccat attatatccc aaaactgttc ccactaaaaa atggccgtat
     5701 tcatggatga taacagttaa taaagaggca ataaataaaa ataagtatat tctatatttc
     5761 ttaattattc gcattcaaat cacctattat atcattattt atttaaaaca aatttgaatc
     5821 atcgttaaaa taaaacaatc aaaataaaaa gagttaacaa attcagttat ttattaggaa
     5881 tttgtttagt taataattat ttgaattcat ataaaccgat cataacacca tcagggtctt
     5941 ctatattgat aaaactacca cctttaatct ggacaggacc ttccttaatt tcgattccct
     6001 tttcttgcaa gaatctaatt gtctggtcaa ggtcttctac cccaaagccc attttaatct
     6061 tggattcttt ggctttgtca gctttgcttt caccgctacg ttctattaat tctaccgtgt
     6121 tttcttcacc atcttctaaa aatataattt tcacgccttc ttccggttct attcttctta
     6181 ctacttccag ttttaaaatt tcagaataaa actcataaga ctgatcgagg tcagccactt
     6241 caatcgtatt aaaaagaaat tccataaaat acaccctctc tgattactta ttattcctta
     6301 attcaacaaa gaagaactta tccccttgtc ttttttctaa aacagaaaag ttataatctt
     6361 ttgctaagtt aataaaagtt tccatactct gctcattatc ctgccagcgg gctccataac
     6421 ccgtattaat agtattttca ttaaagttta cttccacttg tgcggcataa acctcttctt
     6481 tatgcgactg ccgggctgga atagtaaggt cccatattaa aaagctgcgg tttggtttta
     6541 aaaccctttt tacctcttca aatacttgac caatatcatt attttccaag tacatgagag
     6601 taaaaaagga agtaaccatt tcaaattgat tctctaagaa gtacagatta cgggcatcca
     6661 taactatctt caaagcctga ttttcaggtg attccagcag ttcttcctca tcgatatcaa
     6721 tggccacaac attatctcca taaacctgag ccattactgc ttcaccacca ccgccaatgt
     6781 cgaggatcct gcccatatta ttcttctctt caatatcaac tatttgcttg tctgtaaaaa
     6841 atatcttatc ttgatcaata tcagccactc gctaatacct cctcgatatt tgatatgtat
     6901 ttaatatccc aattatataa atcaaagtcg taatatcctt ttttaataat gggatcatct
     6961 ctacttattt tttcagcctc cgcttcacta gcagctgtaa atattaccat gccgccgtcc
     7021 ttttgttcaa atcctcccgc tatcagaaaa ccattatctt ttaactcttc tatatatttt
     7081 agatgtagat tgaacacagg ttcttctatt ataccttccg atactcttct atctattcta
     7141 acaaagatat tatttttcaa tatatttggc tcctttaaga aattttagat ttatccgcaa
     7201 cttctgccag ccttttttca gccagagaaa ctaaaataac ggatacaatt attaaaatac
     7261 tcccggcaaa atggtaccag gccaaaaact cattaaacac taagatacct actattaaac
     7321 ttgtaatcgg ttcaaaagta cttaagatcg atgaagtagt ggcagttatg tatttcaggc
     7381 cggcctgaaa ggaaaccatt ccaaagatat tacaaattag ggcgaccata atagtactaa
     7441 gcaggccttt ataattgtaa tttaaactta tattacccgt aactaaagtt aaagcaaaga
     7501 cgataaaggt attaaataag gaaagataaa aggtaatcac aaaactattt agatccttta
     7561 tgttactatt tgaaaccatt aagatgtaat aactatagag aatacctgat atcagggcca
     7621 accagaaacc aagcatgctt atatttgaaa aagaaccaaa acccattaaa aaatatatac
     7681 cggaaataga taagataagg gcaagcactt ttttgggccc gatcttttct ttatataaaa
     7741 agactgaact taacatcaca aacaccggat aaacaaaatg gagagtcatg gcaaggccgg
     7801 tcggcatata taaaaaagat atatataaga tgaccagcat gatggaataa ccgaacaggc
     7861 tgaccttaaa caatgtctta tattgggcct tcgttacctc catatcaagg ttatatagtt
     7921 ttataaaaat aaataacgca actgcagcaa ataatgacct atacaaggtg aaactatagg
     7981 ggttaaatcc attggcatag gcggttttgg ctaaaaaacc ctgaaatcca aaacctactg
     8041 ctgcaaatat tgttaataaa ataccttttg ttttattcat atttttccac ctaatattat
     8101 aaatttttaa ttcgttatgc tactaaattt tcttaatctc ttttaggttt ctatttaaat
     8161 atttaaataa atatatgtca ggcattctta tatttctcca atgctcataa tcaaaactat
     8221 catcaaagta atttaagatc actcccagcc aggtcccatg accggctact atgatattac
     8281 cctgcttaat gttattaact atcttattta atacttttaa acctctctcc tgtacttcat
     8341 taaaagattc accgcctggt agagaataat caaaatcatt ccactgcttg gctgcaaatt
     8401 caaaaaaatc atctatgtaa tggtccgcaa cttttctttc tcttaaatta tccatcaatt
     8461 caatactgag ttcattatct tctgctaatg gttttacggt atctattgct ctaaaataag
     8521 ggctagcata tactttatca atgcttatat ctttaaatat atttactaat ttttcagcat
     8581 ctttttttcc tttggttgaa agcgggcgat taaagtcttc atcttctgat ctataaatcg
     8641 tttcagcatg tctgataaaa tacaagttca attatcttca ccttctctat ctattataat
     8701 attatcttta atgtattgta gtgctttcac ttcccgtaag gcccaaaaag gtgtctcttt
     8761 ataactttcc ataatttcta tattatttgc aatagcttta ttaatatcta cccaccttgt
     8821 ttctaattcc aattccaact cataatcttc taaatctgaa taacctaatt tttctttaat
     8881 atcagttaga taataatatg aggtcatttt taagatatca tatccctcaa caggggcctt
     8941 attatattca ataatttttc ccagggaatc aatatctgtc gatattatta tcccacattc
     9001 ttctattatt tctcttttta aggcttgaaa acgggtttca cctttttcta ctcccccacc
     9061 tgggaaagca taatcaccgt atttttgaga ataaagcatc aggagttcat tatcttttaa
     9121 tatgactccc cgtactgctt ccctctcaaa tgtttttggg ggaaaatcta aattatcttt
     9181 cttatatata attttattca gctgcattca tcttcctcca cttaacagtt tttatacttt
     9241 tttaaattca attgtttttt cattttcacg ctctaaattt aatggcaaat tagcagtcat
     9301 ttctttaatg cactgctttt ttaaagtatg gtctttaaaa gatagatatc cccccgtttt
     9361 taacatgccg gatatgctct tgacagttcc cctcatatca tttaaatcat gtagagtatc
     9421 atacaggata actacgtcta tactttcttt ttcaatcatt tttatattat ggccgtcaat
     9481 atattctaca ttttctaaat tttcagtttt agctttattc tttactttct caaaagctat
     9541 aggattaata tcaactgcaa ataccttacc cttattacct accatctgag ccaaaggaat
     9601 tgtaaaacgg ccggggccac aaccataatc gaccacatga tcacccggct gaatattagt
     9661 ttgttttaat atctcttctg gaggagaaaa tattgacttt atcttatact cagccgccat
     9721 taaattaaaa ctgattttat ctatcaaacc aacttccttc attttttgtt ctctcctttt
     9781 taagttatag gataaaataa ggaatttaaa aagttggaaa atgcctaatg ccttttgtat
     9841 atctttaaat atttctgcta attctttttt gcagccttac ccctatattg acaacagcca
     9901 tatagaggtt agccagaatt acaaaagaaa ctagactgcc cagaaccttt atttcaaata
     9961 aattagaaag tcctatcaca agactaatta aggaaactgt ccatactaat aattgggcaa
    10021 aagagataat tttttgttga atatcatttc taaaggtata ataactaatt aaaagggcta
    10081 agaccaccgt cactaaagcc caaaaattat aaaacatatt tgaatctctc cctgtttatt
    10141 attgttttta ataatttcag atataatatc tcttaattat tcttattctc tatttttaaa
    10201 tcttttcctt ttattaattc atcatgatta tctattttaa taagataagg gttattgata
    10261 gataaattaa catctacaca ataacccctt tagactgact aatctaatgt atttaattct
    10321 ttggaacgaa gggcagcttc cttcacagca tcaattagta tgcccctaac tccgtgtttt
    10381 tctaatactt ctatacctcg aattgaagtc ccccccggtg aggtaaccat atcttttaaa
    10441 acaccgggat ggttttctgt ttctaaaacc atcttggcag aacctaacat ggtctgagca
    10501 gttaatttta aggccatctc cctggaaatg ccggttagta ctcctccatc agctaaagct
    10561 tcgatcatga tatagatgaa agcaggtccg ctaccgctta aaccggttat ggcatccata
    10621 agattttctt ctacttctac tacttccccg actccggcaa atatcctatt aaccaactta
    10681 atatcttctt cagcggcatt tcggccgggt gcaatcgctg aaataccgga cttgactaag
    10741 accggagtat taggcattac ccggatggtc ctcacatcta atggtaattt ttctttgagg
    10801 tgagaattgc tgatcccggc cgcaatagaa attatcagtt tgcctttagc tgcttcactg
    10861 atctcttcta agacatggtc tattacctga ggtttaatgg ctaatagtac ggcatccgcc
    10921 tgttctactc catatacgtt atcatctgta gtttctatac caccataatc ttcattattt
    10981 ttagtttctt tagcttttat atcacagccg ataagctcat ctgcttcata aagtcctgaa
    11041 tttaatatac cctgcattaa agctgtacct attttaccga gtccgattat acataatttc
    11101 atctttatca ctactccctt atttgacctt gaccgtaaat cacatattta gttgaggtta
    11161 attcattaag tcccatcgga ccacgggcat gcatcttctg ggtgctaatt ccaatttcgg
    11221 ctcccagacc aaactgatta ccatcggtaa atctggtaga ggcatttata tatactgcag
    11281 cagcatcaac ttcatttagg aactgttggg cattagtata atcttccgta ataattgcct
    11341 ctgaatgttt ggtgccatat ttgtttatat tttctactgc atgatctata ttatccacaa
    11401 cttttagtga tataatgtag tctaagtatt ctttgcccca atcactttca tcggcttttt
    11461 taatactgtt ttcaatcttg actgcggtgt catcgcctct tatttctaca tcatattcgg
    11521 ataaaacctt ggctattcgg gggataaaat cattcgctag ttctcgatgg attaataagc
    11581 tttctgctgc attacagacc gccggacggc tggttttggc attaactata atttcagctg
    11641 cttttttaat atcagcagtt tcatcaacaa aaatatgaca gttacctact ccggtctgaa
    11701 taactggaat cttcgattca ttaataaccc ggttgattag ttctgcccca ccgcgcggga
    11761 ttaataaatc aaggtaatca tttagagtca ataactcctt taccgctgcc cgatccgtag
    11821 tttgaattaa ttgtaccgtt ccggccggca tacctgcttc atgggcatta gcctgaatta
    11881 tctcagtcaa aattttattg gaattaaagg cgtttgaacc tccccttagt aggactgtat
    11941 tacctgattt aaggcataag gccgctgtat caacggtcac atttggtctt gcttcataaa
    12001 tgataccaat tacacctaag ggaaccctca tcttacctat ttgtaagcca ttgggtctct
    12061 ttttcatttc tagaatatca ccaatcggat catctaattc agctacttcc attaatccat
    12121 cagccattcc tttaattcga tcttcatcaa gagataaccg atctaaaaat gctttagaaa
    12181 ggttctgttc tctagccttt tccatatctt tattattagc cgctaaaatt tcagattgtt
    12241 tattcattaa accttctgct atattatata gggctttatt tttaacttcc gttgatatgt
    12301 tagcggtctt taaagaggct tcttctgcta atttagcctg acttactact tcttctctaa
    12361 tactcataat attctctcct ccttttagtg attagaaatt tttcttttac actgtttatc
    12421 attcggtaaa aaagtagtcc ctattttata ctcactatcc tctttaacca tctcaatgat
    12481 tttactaact acgttatcac ggtaagcggg accgatgacc atcgtgatcc cagcctcatt
    12541 aacaatttta gccgcttcta gttttgtttt cattcccccg gttccgacaa tactgccttc
    12601 atcaccggcc ttttcttcaa tatccggtgt aatattttct actttattga tcagttctaa
    12661 atcctcttcc gtatcacagg gaatcttatt atacatgcct tccacatccg ttagtaagat
    12721 caacaggtct gctttgatta gtcggctgac ccgggctgat agggtatcat tatcaccaaa
    12781 cctgatctca tcagtggcaa ctgtatcatt ttcattaata attggtatta tgtcattttt
    12841 aagtaatact tccaaggtgt tgtatatgtt tttagctctt tctttttcct ttaaatcact
    12901 ggaggtcagt aaaacctggc cgcctaattc cccgtactgt cttaatattt tgttatataa
    12961 agagattaat agtccctggc cgacggcagc cattccctgc ttttcagcca tgctgcaggg
    13021 taagttcaat ttgagttctc caatcccggc cccgattgca cctgacgata ccagtaaaac
    13081 ttcttttccc atgtttttta gatctactag ctgccttaaa tacttttcaa ttgccgtata
    13141 gtttaatttg ccgttatcat gtgtcaaaca gcttgtcccg atcttaacca ctactcgctt
    13201 gaaatcattt tcttgcacag tactcactcc tcattgataa tatttaaaaa attcttagag
    13261 aatttaaaaa accccgttcc ttgagtagga acgaggttga attcgcggta ccatccttgt
    13321 tgactttatg tagtccactt aattacagtt acaaaagtac taaaacttct ataacctgtc
    13381 atgataacgg ttgactgccg gcaaagctta ctaatttcag cttccaactc ccaggcgggt
    13441 ttcattaaaa tgaatattac cgggcttcca ccatccccga ctctctcgta aatatcactt
    13501 taattactat tcctgttcac tgtcaataaa ctatatttta ttttgattac ttataataat
    13561 acataatgaa tataaatatg tcaagtaatt taggaattta tacctaaaaa ttcatttttt
    13621 tgaaaattaa gtagctattg ataaatctat tttccaattt ccaattataa t
//
LOCUS       DATGCJ010000087         7202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_591279, whole genome shotgun sequence.
ACCESSION   DATGCJ010000087 DATGCJ010000000
VERSION     DATGCJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591279"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VKN64_04575"
     CDS             <1..318
                     /locus_tag="VKN64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMA59548.1"
                     /translation="LKFGEAQEIVQYLRRKFDLIFIDAAKGQYLNLFKIAEEKITDNG
                     VIICDNVLYKGLVRDNQRVDHKIRTMIVNMRKFLIYLKNNKRFKTEILDIDDGITISS
                     RRN"
     gene            321..1544
                     /locus_tag="VKN64_04580"
     CDS             321..1544
                     /locus_tag="VKN64_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HMA59549.1"
                     /translation="MDKVELLAPAGNLEKLKVAALYGADAVYCGGFSYGLREGADNFS
                     YEELKEGTEFLHARDKQIYVTVNMIPHNEDLEGLPKYLLQLEEIGVDALIISDPGVLN
                     IIREENIEIPLHLSTQANAVNYASVNFWHELGMDRIILARELSQAEIKEIRDNTEISL
                     EMFIHGAMCISYSGRCLLSNYFVNRDANRGQCAHSCRWKYYLMEEERPGEYYPVFEDK
                     NGTHIMNSKDLCLIEYIPEVIETGVDSLKIEGRMKGMHYVANVVSTYKKAIDVYYADP
                     DNYEFQEKWLKDLRKVSHRGYTTGFFLHPPTEEDQNYESSAYKRTFDFMGVVRGYDAE
                     KKEVLIEVRHKFFKGDTIEILKHNGQVQTETVDYIINEEGEKVEDAPHPKEIIRVPIE
                     NEYEELEVVRREVRE"
     gene            1544..1765
                     /locus_tag="VKN64_04585"
     CDS             1544..1765
                     /locus_tag="VKN64_04585"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HMA59550.1"
                     /translation="MEKNTHRIVAQVPQEEIVFVDMIFKSFEGIAMLTVSKEEPGEIY
                     LDVTDGTEDLVIEILEDLNNEFPVQIKSK"
     gene            complement(1819..2304)
                     /locus_tag="VKN64_04590"
     CDS             complement(1819..2304)
                     /locus_tag="VKN64_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMA59551.1"
                     /translation="MKNFVKKSKNNKGFSLIELLVVIVIAGIVITSASSLFVSAFNYF
                     TQNKEKAENQRELRFITNYISENIKFSNDVSIKSSTSTPPSGYKGIGLDNNYVKIFNS
                     DGTSRKLSNIIIDNLTFDPSGKSLDVEIEKNNYSIDTKVYLNNHKLSGTTSGIFIEFE
                     E"
     gene            complement(2330..2692)
                     /locus_tag="VKN64_04595"
     CDS             complement(2330..2692)
                     /locus_tag="VKN64_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMA59552.1"
                     /translation="MKFYDEGFTLVEVLVTLLLLGIIAVGIFPVFGNIFSSIFSAGDK
                     TNATYVNKEKIIDSIKNENNIKDSNLSIIVSTDPNDTSDDYEISSNGALQEVTTEYSK
                     PNIDTKEDVTMQYYITTP"
     gene            complement(2718..3839)
                     /locus_tag="VKN64_04600"
     CDS             complement(2718..3839)
                     /locus_tag="VKN64_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59553.1"
                     /translation="MKFIEKIKKDDGFAMVFGLILILVVITMATVLINISINEYSEAE
                     RNKNEIKAYFLARSGAEIMANKLEKESSIRTYADSNLVGEEFTIENDTNNEVISTISL
                     SKDAEGNYNITSTAEVQGVTETIKVSVSKLKLFQNAIFAKQNIDLSALDLLDVLTDGS
                     SSVESNGTVKDPNDFLKDENIIEYSDKKFTEPTFPSPKNGKIKNDTISESGTFKKIKY
                     NNGELTIDFSDDKNNNEINIVVDSMTFKASMNFVDNDDGENVSDAVLNIFVNKDITFQ
                     TPNVDTKQFNIFLKSGAEMTLVGNPDTTGEAVYVYGPNANMYMNSAKTSFKGAVIVDR
                     IYRNTSQPAFKGVFEHKKPTNLDIFDDYVKSYQVSDWRR"
     gene            4070..4564
                     /locus_tag="VKN64_04605"
     CDS             4070..4564
                     /locus_tag="VKN64_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HMA59554.1"
                     /translation="MKKLYPDIYYESIFDIDIQDFKDQDIKGIIVDIDNTIVPWNDKE
                     LSDEVFNWFKKLIKEGYKLCLISNGMSKRVEYFSNQLDIPAIGTAVKPRKKAFRKALN
                     ILDVKKNKIAVIGDQIFTDVLGGNRMGFTTILVDPLNENEFITTKIMRFLERLVIKRG
                     MYHD"
     gene            4582..5274
                     /locus_tag="VKN64_04610"
     CDS             4582..5274
                     /locus_tag="VKN64_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HMA59555.1"
                     /translation="MIYRSLKKMLTIVVIALFILVSYVSVLKAEDFNELGNRVLQYGD
                     EGSDVAILQQRLSHLEFYKGKIDGIYGKKTEEAVQRLQKAYGLTVDGIAGKSTYKYLP
                     NNNIYHRMDITRDQIILLARVIHGEARGESFAGKVAVGAVILNRVDDKNFPSSIREVI
                     LQRGQFSSLIDGQANLFPDQKSINAARAALVGYDPTHESLYFYNPDVATNISWISKRP
                     ITLKIGDHVFAR"
     gene            complement(5330..5581)
                     /locus_tag="VKN64_04615"
     CDS             complement(5330..5581)
                     /locus_tag="VKN64_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMA59556.1"
                     /translation="MKSTGIVRKIDDLGRMVIPIELRKTMNINKKDPMEIFVEDDKII
                     LKKYEPACIFCGSADNTFEYKGRTICQSCMEKMEEKAKE"
     gene            5755..6201
                     /locus_tag="VKN64_04620"
     CDS             5755..6201
                     /locus_tag="VKN64_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMA59557.1"
                     /translation="MSEKNNFEKFKKILIENNYRLTKQRKELLKVFLKYKEKHFNAEQ
                     LLDILKNRDESIGLATVYRNLELFHRLGILKKLDFNDEFKYYELNLIEKHHHHLICIN
                     CDRIIEFNDEALSDFEQQLEEEYDFEIKDHIIKFYGVCKKCSEEGE"
     gene            6205..6438
                     /locus_tag="VKN64_04625"
     CDS             6205..6438
                     /locus_tag="VKN64_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59558.1"
                     /translation="MAEDQEKMIICPLCGHNFTKGESQKGCEGCPLHKNNCGLIRCPN
                     CGYEFPEVDDLSLMDRIRKFFKINYDKDDKGGQ"
     gene            6441..7133
                     /locus_tag="VKN64_04630"
     CDS             6441..7133
                     /locus_tag="VKN64_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMA59559.1"
                     /translation="MEELQSCLKDLTVFSKLKPDELELVCQSSYEKHYQKGEIIFFEN
                     DEYGGLYLLVSGRVKLSMLSPEGKEKVLNILQEGDIMGEISFFDLDPHPITAEAMEKA
                     RIVIIPREKLEEIITDKPGLALKIIESLAKKTRLLTSQVRELVFHDAAGRMASLLRRF
                     ADDFGVEINGGKMIDLILTHKEIANLIGSSRVTVTKIMNKFIDEGIIKMYKRKIVIID
                     EEKLGKKLHTSI"
BASE COUNT         2496 a         1021 c         1296 g         2389 t
ORIGIN      
        1 ttaaagttcg gtgaagccca ggaaattgta caatatttgc gtagaaaatt cgatttaatt
       61 tttatagatg ctgccaaggg gcagtatcta aatttattta aaatagcgga agaaaagata
      121 accgataacg gtgttataat ttgtgataat gtattgtaca aaggtctggt cagagataat
      181 caaagagttg atcataaaat taggacgatg attgtaaata tgagaaagtt tttgatctac
      241 ttgaaaaaca ataagcggtt taaaaccgaa attttggata tagatgatgg aattactatc
      301 agcagcagga ggaattgata atggataaag ttgaactact ggccccggcc ggcaatctag
      361 aaaagttaaa agtagcagcc ctttacgggg cagatgcagt atactgtgga ggttttagtt
      421 atggcttaag agaaggtgct gataatttta gttatgaaga gctaaaagaa ggtactgaat
      481 tcttacatgc aagagataaa cagatctatg ttacggttaa tatgatccct cataatgagg
      541 atctggaagg tctaccaaag tatttactcc aattagaaga aatcggagta gatgccttaa
      601 ttatttccga ccctggggta ttaaatatta ttagggaaga aaatatagag ataccattac
      661 atttaagtac ccaggctaat gcagttaatt atgccagtgt taacttctgg catgagttag
      721 gtatggatag gattatctta gcccgggaat taagtcaagc tgagataaaa gagattagag
      781 ataatacgga gatatcatta gagatgttta ttcacggggc aatgtgtatt tcctactctg
      841 gtcgatgtct gttaagcaac tattttgtca acagagatgc caaccgtggt caatgcgccc
      901 attcctgccg ctggaaatat tatctgatgg aagaagagag accgggtgaa tattaccctg
      961 tttttgaaga taaaaatggt acccatatca tgaattctaa agatttatgt ctgattgaat
     1021 atataccgga agtcattgaa acaggtgttg atagtttgaa gatcgaggga cggatgaaag
     1081 gtatgcatta tgttgctaat gtggtatcta cttataagaa agcaattgat gtctattacg
     1141 cggatcctga taattatgaa ttccaggaaa aatggttaaa agatttaaga aaggtaagtc
     1201 acagaggtta tacaaccggc tttttcctac atcctcccac tgaggaagat caaaattatg
     1261 aatcctctgc ttacaaaagg acctttgatt tcatgggggt tgtgagaggt tatgatgctg
     1321 aaaaaaagga agtattaata gaagttaggc ataagttctt taagggagat accatagaga
     1381 tcttaaaaca caacggccaa gtacagactg aaactgttga ttatattatc aatgaagagg
     1441 gagaaaaagt ggaagatgct cctcatccga aagaaataat cagggtgcca attgaaaatg
     1501 aatacgaaga attagaagtt gttagaagag aggtaagaga gtaatggaaa aaaatacaca
     1561 ccggattgta gcacaagtac cgcaagaaga gattgtcttt gttgacatga tctttaaatc
     1621 ctttgaaggt attgcgatgc ttacggttag taaggaagaa ccaggtgaga tataccttga
     1681 tgttactgat gggacagagg acttggtgat tgaaatttta gaggatttga ataatgaatt
     1741 ccctgttcaa attaagagca aataaaaaac ctcagccacg ttttttttaa actatggctg
     1801 agattataaa tctttttttt attcttcaaa ttctataaaa atacctgaag tagtaccact
     1861 caatttgtga ttatttaaat atacttttgt atcgatacta taattatttt tttcaatttc
     1921 aacatctagt gatttacctg atggatcgaa agtaaggtta tcgattatta tattagataa
     1981 ctttcgtgaa gtaccatcac tattaaatat tttaacataa ttattgtcta agccaatacc
     2041 tttataacca ctcggtggtg ttgaggttga acttttaatt gaaacatcgt tggaaaattt
     2101 tatattttca ctaatatagt tcgtaataaa tcttagttct ctttggtttt cagccttttc
     2161 tttattttga gtaaaataat taaatgctga tacgaataat gaagaagcag atgtaattac
     2221 aattccagca ataactatta ccactaataa ttctattaaa gaaaagcctt tattattttt
     2281 tgatttttta acaaaatttt tcataatata caactcccta aattaataac taaggtgtgg
     2341 taatataata ttgcatagtg acatcttctt ttgtatcaat attaggttta ctatattcag
     2401 tagtgacttc ttgcaatgca ccattactac ttatctcata atcatcactt gtgtcattag
     2461 gatcagtaga aacaattata gataaattac tatctttaat attattttca tttttaattg
     2521 aatcaataat tttctcttta tttacataag tggcattagt tttatcccca gcactaaata
     2581 tactgctaaa aatgttgcca aaaacaggaa agattcctac agcaattatt cctaataata
     2641 ataaagtaac taaaacttcg acaagtgtga agccttcatc ataaaatttc atattatctc
     2701 acttcctgat ttaagaatta tcttctccag tctgacactt gataggattt cacataatca
     2761 tcaaagatat ctaaattagt tggtttttta tgttcaaata ctcctttaaa agctggttga
     2821 gatgtgtttc tatatattct atctacaata acagcacctt taaaggaagt tttagcacta
     2881 ttcatataca tattagcatt gggaccatat acatagacag cttcacctgt agtatcagga
     2941 ttaccaacca aagtcatttc cgctccgctt tttaaaaaaa tattaaactg tttcgtatct
     3001 acattaggtg tttgaaaagt aatgtcttta tttacaaaga tatttaatac agcgtcactt
     3061 acattttctc catcgtcatt atctacaaaa ttcattgaag ctttaaaagt catactatca
     3121 acaacaatat taatttcatt attattttta tcatctgaaa aatcaatagt taattctcca
     3181 ttattatatt taattttttt aaatgtaccg ctttcagaaa tagtatcatt ttttatttta
     3241 ccattctttg gtgaagggaa ggtaggttcc gtaaatttct tatcggagta ttcaatgata
     3301 ttttcatctt ttaagaaatc attgggatct ttaactgttc catttgattc aaccgaactt
     3361 gagccatcgg ttagtacatc tagtaaatca agtgcgctta agtctatatt ttgtttagca
     3421 aaaatagcgt tttggaataa ttttaattta ctaactgaaa ctttgattgt ttcagttact
     3481 ccctgtactt ctgcagtaga ggtgatatta taatttcctt cagcgtcttt acttaaagat
     3541 atagtactta taacttcatt attggtgtcg ttttcaatag taaattcttc ccctaccaaa
     3601 ttactgtctg cataagtacg aatagaactt tctttttcta gtttgtttgc cataatttca
     3661 gctccagaac gggctaaaaa atatgctttg atttcatttt tgtttctctc tgcttcactg
     3721 tattcattta ttgatatatt tattaatact gttgccatag taatcacaac taaaattaga
     3781 attaaaccaa acaccattgc aaaaccgtca tcttttttaa ttttttcaat aaatttcata
     3841 ttaatcagtc ctttactaaa aggatataaa gattatttaa ttttttaaat atttttatac
     3901 ttattgtaat aatatgacat attaacgcaa ctgtcaatat agtatttaac aattatctat
     3961 atagcagcag taattaaaaa attgtatata tctataatat ttgacaagta gtacttaatt
     4021 agttatgatt actttatgaa acaagttgta aatttcgggg gctgttatct tgaagaaatt
     4081 atatccagat atctactatg aaagtatatt tgacattgat attcaagact tcaaagatca
     4141 ggatattaag ggtataattg tcgatataga taatacaatt gtcccttgga atgacaaaga
     4201 attaagtgat gaagtattta actggtttaa aaaactaatt aaagaaggtt ataaattatg
     4261 ccttatatct aacggaatga gcaaaagagt tgagtacttc agcaatcaat tggacatccc
     4321 ggccattgga acagctgtga agcccagaaa aaaagcattt agaaaagctt taaatattct
     4381 tgatgttaag aaaaataaga ttgcagtaat tggggatcaa atatttactg atgttctggg
     4441 cggcaacagg atgggtttta ccactatttt agtggatccc ttaaatgaaa atgaatttat
     4501 aacaacaaaa atcatgcgtt tcttggaacg acttgttata aaaagaggga tgtaccatga
     4561 ttgattattg gagggataat tatgatttac agaagtttga agaagatgct gacgatagtt
     4621 gtaatagcat tgtttatatt agtgagttat gtgagtgtac tgaaagcgga agactttaat
     4681 gagttgggaa atagagttct acagtatggt gatgaaggtt cagatgttgc aattttacag
     4741 cagagattaa gtcatttaga attctataaa ggtaagatag atggtattta cggtaaaaag
     4801 acagaggagg ccgtgcaacg gctccaaaaa gcttatggac taaccgtaga cggaattgcg
     4861 ggtaagtcca cttataaata tcttcctaac aataatatct accatagaat ggatattaca
     4921 agggatcaga tcatactctt agcgagagtt atccacgggg aggcccgcgg agagagtttt
     4981 gccggcaaag ttgccgtggg agctgttatc ttaaacagag ttgacgataa aaatttccca
     5041 agttcaatta gggaggtaat tttacagaga ggtcagttca gcagtctcat agatggacag
     5101 gccaacctat ttccagacca aaaatcaata aatgctgctc gagcagctct ggttggttat
     5161 gatcctaccc atgagtcact atatttttac aatccagatg ttgcgaccaa tataagctgg
     5221 atatcaaaaa gaccgataac attaaaaatt ggagatcatg tatttgcaag atagatatat
     5281 aaaaaaagta gcaccctgtt tttgatacca ggtgctactt ttttgtattt cattcttttg
     5341 ctttttcttc cattttttcc atacagcttt ggcaaattgt tctaccctta tattcgaaag
     5401 tgttatcagc acttccacag aatatacaag cgggctcata tttttttaga ataatcttgt
     5461 catcttcaac aaagatttcc atgggatctt ttttattaat attcatagtt ttacggagtt
     5521 caatcgggat caccattcta ccaaggtcat ctatctttct aacaatacca gtagacttca
     5581 aattcccacc tccttaatta gaaaacagta ttttattttt atattgtgta ataattatac
     5641 tatttattac atataaagtc aatagtttac attttattag tattcatgat attattataa
     5701 tagaatcttt aaaatgtttg ccttgttgta attttccaaa ggaggaagcc ccttatgagc
     5761 gaaaaaaata attttgaaaa atttaagaag attttaatag agaataatta tagattgacc
     5821 aaacaaagaa aagaattatt aaaagttttt ttaaaatata aagaaaagca ctttaatgct
     5881 gagcaacttt tagatatact taaaaataga gatgaaagta ttggtttagc gactgtctat
     5941 aggaacctgg aattatttca ccgtcttggt attttaaaaa agcttgattt taatgatgag
     6001 tttaaatatt atgagttgaa tttgattgaa aagcaccatc atcatttaat ctgtataaat
     6061 tgtgatcgaa tcatcgaatt caatgatgaa gcactcagtg attttgaaca gcagttagaa
     6121 gaagaatatg attttgaaat caaggatcac attattaaat tttatggtgt ttgtaaaaaa
     6181 tgctcagagg agggtgaata gttaatggca gaggatcaag agaagatgat tatctgtcct
     6241 ctctgcggac ataattttac caagggagaa tcacaaaaag gttgtgaagg ctgtcccctt
     6301 cataaaaaca actgtggatt aattcgctgt ccgaactgtg gatatgaatt tccggaagta
     6361 gatgatctat cattaatgga tagaataagg aagttcttta aaataaatta tgataaggac
     6421 gataaaggag gccaatagat atggaagaac tgcagagctg tctaaaagac ttgacagttt
     6481 tttcaaaact aaaaccagat gaactcgaac tagtttgtca gagttcttat gaaaaacatt
     6541 accaaaaagg agaaatcata ttttttgaaa atgatgagta cggtggttta tatttacttg
     6601 tttcaggtag ggtaaagctt tccatgcttt ccccggaagg caaagagaag gtgttaaata
     6661 tcctgcagga aggcgatatc atgggtgaga tctcgttttt tgatttagac cctcatccaa
     6721 ttactgctga ggcaatggaa aaggccagga tagtaattat tccgcgtgaa aaactggaag
     6781 aaataattac agataaaccc ggtttagcct tgaagataat tgaatcactg gccaaaaaga
     6841 ccagactgct taccagtcag gtaagagaat tggtctttca tgatgcagcc ggccgcatgg
     6901 cgagtttatt aagaaggttt gctgatgact ttggggttga aattaacggt ggtaagatga
     6961 tagatttaat tttaactcat aaagagatag cgaacttaat cggttcatcc cgggtgactg
     7021 taacaaagat tatgaataaa tttatagatg aaggtataat taagatgtat aagagaaaga
     7081 tcgttattat tgatgaagag aagttaggaa agaagttaca tacatcaatc taatcaggga
     7141 ctgatctagt tgtctaataa agataataaa tacaagatgc tcaaagcaga tttatcatta
     7201 at
//
LOCUS       DATGCJ010000088         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_458824, whole genome shotgun sequence.
ACCESSION   DATGCJ010000088 DATGCJ010000000
VERSION     DATGCJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458824"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VKN64_04635"
     CDS             <1..1038
                     /locus_tag="VKN64_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59560.1"
                     /translation="RNSNYWKRDPQGKPLPYIIRWVRVISESQETQALMFEKGQTHAL
                     GVRGIDFDRFKESADEGNYHMVNGGPTFGSNFLIFNMNPRNPKLEEQPWKYEWFTNLH
                     FRRAVAYSMDKDTMIDQALAGYGSKQWSPISEPNKVYLNEEVKKYPYSLEKAREELEA
                     GGFSWDDNGNLVDKDGRRVEFNMVTNAGNTQREAILNIVATELRELGMKINSTPIEFN
                     KLVNQLMTEWNFDTIIIGLTGGVEPHSGANVWPSHGHLHMWNPQQEEPATEWEARIDE
                     LFEKGASATKVEERVKYYDEFQEIIAERVPVIFTVTPNALYAVRNTLKNTEPTAYGEM
                     WWNISEQYITD"
     gene            1147..2115
                     /locus_tag="VKN64_04640"
     CDS             1147..2115
                     /locus_tag="VKN64_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59561.1"
                     /translation="MRTYILRRLLQAIPLLLAISLLSFFIMQLSPGSYLDTMKLNPDV
                     SEELIENMKRQYGLDKSVPEQYFYWLWQILQGNFGESFTYQVPVTHVIKTRVVNTMIL
                     SVAAMFIAWGFSIPIGIYSATHKYTVGDNIFTSLAFVGLSIPNFFFALLLLFLIVKFN
                     VPLPINGMTSIMHDFMSPMGKIIDILKHLLVPAVVLGTASMATLTRIMRGQMIEAMRS
                     DYIRTAKAKGLKNRKVVYKHAFRNAINPMITIFGFQISMLLSGAALTETVTGWPGLGK
                     MMLTAVRSKDLYLAMAGLMIGSVLLIIGNLIADVLLAVSDPRIRYS"
BASE COUNT          728 a          358 c          449 g          671 t
ORIGIN      
        1 agaaactcca attattggaa aagagaccca cagggtaaac ctcttcccta tataatcaga
       61 tgggtaagag taatctctga aagtcaagaa acccaagctt taatgtttga aaaaggtcaa
      121 actcatgccc ttggtgttag aggtattgac tttgatcgct ttaaagaaag cgcagatgaa
      181 ggtaactacc atatggttaa tggtggacca acctttggat caaacttctt aatatttaat
      241 atgaacccac gtaatccaaa attagaagaa caaccctgga agtatgaatg gttcacaaat
      301 ctacatttca gaagagctgt tgcttattct atggataaag atactatgat cgatcaagct
      361 ctagctggtt atggttctaa gcaatggagt ccaatcagtg agccgaacaa agtatattta
      421 aatgaagaag ttaaaaaata tccatacagt ttagaaaaag ctagagaaga acttgaagct
      481 ggtggatttt catgggacga taatggtaat ttagttgata aagatggtag gcgtgtagaa
      541 tttaatatgg ttacaaatgc tggtaatact caaagagaag ctattttgaa tattgttgcg
      601 accgagttaa gagaattggg tatgaagatc aattctaccc ctatcgaatt caataaatta
      661 gttaatcaat taatgaccga gtggaacttt gatactataa taatcggtct aaccggtgga
      721 gtagaaccac atagtggggc aaatgtatgg ccatcccacg gacacttaca catgtggaat
      781 ccacagcagg aagaacctgc taccgaatgg gaagctcgta ttgacgaact gtttgaaaaa
      841 ggtgctagtg ccactaaggt ggaagaaaga gttaagtatt atgatgagtt ccaggaaatt
      901 attgcagaaa gagttcctgt tatcttcacc gttactccta atgctcttta tgctgtaaga
      961 aatacactta agaatacaga accgactgct tatggagaaa tgtggtggaa tatttctgag
     1021 cagtacatta ctgattaata aggactaaca atttaaatta aaatagagag aaaggggata
     1081 tcccctttct ctcttcaagt taatatattt ttttattttt attttattga aaggagtacc
     1141 caattaatgc gcacatatat tttaagaaga ctcttacagg ctataccttt acttctggca
     1201 atatccctgc tctccttttt cataatgcag ttgtctcccg gtagttattt agatactatg
     1261 aaactaaatc ctgatgtatc ggaagaactc attgaaaata tgaaaagaca gtatggtcta
     1321 gataaaagtg taccggaaca gtatttttac tggttgtggc agatcttgca gggtaatttt
     1381 ggagaatcat ttacttatca agtccctgtt acccatgtta ttaaaacgcg ggtagtaaat
     1441 acaatgattc tatccgtggc ggcaatgttt attgcctggg ggttttcaat tcccattgga
     1501 atttattcgg ccacccataa atatacagtc ggagataata tatttacaag ccttgctttt
     1561 gtgggattat cgattcccaa tttctttttt gcacttttac ttttattttt aatagttaaa
     1621 tttaacgttc ctttgcccat aaacgggatg acaagtatta tgcatgattt tatgtcacct
     1681 atgggtaaaa ttatagatat attaaaacat ttgctggtcc cagctgttgt actgggtact
     1741 gccagtatgg caactttaac ccgtataatg aggggtcaga tgatcgaagc aatgaggtca
     1801 gattatatac gtacagctaa ggcgaaaggt ttaaaaaata gaaaagttgt ctataaacat
     1861 gccttccgaa atgcgattaa tccaatgatt acaattttcg gttttcaaat tagtatgctt
     1921 ctcagtgggg cagccttaac agaaacggtc actggatggc cgggcctggg taaaatgatg
     1981 ctaactgctg tccgctcaaa ggacctctat cttgcaatgg caggtcttat gatcggaagt
     2041 gtcttactta tcattggtaa tttaatcgca gatgtcttat tggcagtcag tgatccacgc
     2101 ataagatata gttaaggagg gtggaaatgg ataaaataaa tcaaaaaccc agaaatttaa
     2161 aatcaggcaa taataatgaa tatgaatttt ggaatgaaac ctggaa
//
LOCUS       DATGCJ010000089         7563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_279179, whole genome shotgun sequence.
ACCESSION   DATGCJ010000089 DATGCJ010000000
VERSION     DATGCJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279179"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VKN64_04645"
     CDS             <1..530
                     /locus_tag="VKN64_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59562.1"
                     /translation="LGVIEAGNFQGLHDTLDKYLDLVSIITKVPRIELNRMGSSGFGT
                     GGVPTGIALRTIYQSFIGKTNEKSNLIKSGLENTAKKLFKMAEVDNIDTKFEYQEFKT
                     KIHIRSGLPSDDKEKMEVQEKEINNKIKSRETVMQERGIEDVQKEKELIKNELSEDDI
                     YSDRIAQELSIEEDI"
     gene            530..1399
                     /locus_tag="VKN64_04650"
     CDS             530..1399
                     /locus_tag="VKN64_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59563.1"
                     /translation="MIYKKAEIIQKEYRKQYLKKITEFETNYDKILYKYLDRIRKTFF
                     DNSNGSGQIKQRSKNKVKLSLKVLNNWLEIETENILDGCISKTLKIAVLGQLKTLQYV
                     LKYINKTDNISKYRSNLQVIKNEYSSEKIKKIKNYIWKREWEDNLNIDDRLRNLNKKH
                     NKEIDAVINSDHKTVSAYNEIKNTTTNPAKQTYKYNISRLALTETNSGYRYVQKEIGE
                     KSNLVEGIKWNLSASHPKYDYLEICERYALKDHSGLGTGVYRPQNLPILPHPNCLCYL
                     TYFYKNNIIITHL"
     gene            1642..2283
                     /locus_tag="VKN64_04655"
     CDS             1642..2283
                     /locus_tag="VKN64_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59564.1"
                     /translation="MDKVLEILKNNDIEVTEEIKESLNNLAEEGNETKTPLDQTEVNK
                     IVKDQLDQERSAHESEIEKLKTEMKDLVDPEKVEEYESKIKGLEESKDRLKSGLIKDY
                     ELKMAALKEGVEDIEYFEYLVEKNQVKDKLRLNDDNILNVTDQDGNFLTEGGKKVGVE
                     MIISEMKEEKPDLFTGGGKKNTSGPTNPDSSVDLDEYRRENTKKIAEEMGYAK"
     gene            2299..2871
                     /locus_tag="VKN64_04660"
     CDS             2299..2871
                     /locus_tag="VKN64_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59565.1"
                     /translation="MTLKPRESEKTEFIQILKSKHARYIDGMVVVDKDSAVDEYIPPG
                     AALGQITSSGKYGPVTRDKVDTGGADTTDNLIPLKNLDNNKWSNWQVGDEVICDPGET
                     NEETAVITSIDCEIGELTVDGITVEHAEDVIVQKNDGSSKAEIVCLELIDVSLEDAVI
                     GGLLHGAVFSDRMPNYDSIVAEDLPQINFE"
     gene            2886..3932
                     /locus_tag="VKN64_04665"
     CDS             2886..3932
                     /locus_tag="VKN64_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HMA59566.1"
                     /translation="MNKNQLIEIIEDRLGIDYLGFIEEIGEPEKFIGNKYLPAKNEHD
                     YDWVYHIFDNTVAMAKMMARGDAETPIVGGPAVKKVAGSVAPFGQKFEVNKSILNKIF
                     NPRNDNELKSNLRRILDQSARNVRSAQARREWLRFQVLAKGSITLQDRNENNVISVDF
                     GLPGNHKIDSSALEGDAWDGSSPKPLSDLINACDIYYETNDEMPGEILLRKAQAKQIT
                     GSAEVAGEFSENATRISLGEVNDYLAELGYPPIKVHDVKVNTEGPNGRPTNSEYLIPD
                     NRVVLVKKAGGQEIKDTGRLVMGPVSENNFKPGIFTTMYEELDPMRYWHFMKAEMWPA
                     VYNPEYILFMDVQA"
     gene            3957..4226
                     /locus_tag="VKN64_04670"
     CDS             3957..4226
                     /locus_tag="VKN64_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HMA59567.1"
                     /translation="MLVKADWKVGYPTGKERVIYSPADKFKCDEDWGKKKAAAGKITV
                     IKEEIINKEMSVKQLKDKAKKLSISGYSSMKKKELIEHIKGHTDG"
     gene            4219..4503
                     /locus_tag="VKN64_04675"
     CDS             4219..4503
                     /locus_tag="VKN64_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59568.1"
                     /translation="MDKVQKLRHLVWDIGKNVFTDDELLKEFLEDCSGNVYNAAALVL
                     DVVRADPERITEYRRGGVSVSKQNLDKAVSKYEEIGGSLIMTVKEKRVFE"
     gene            4500..4835
                     /locus_tag="VKN64_04680"
     CDS             4500..4835
                     /locus_tag="VKN64_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59569.1"
                     /translation="MRIPRDKSFVEVEIERYTGGSTDLTSGQWQDAELVLVADAEIDI
                     QPKTGSETDSFSGSSYNKRLAGFIGIDDITFSTGFSMIMQGDLVDQKYKVVDIKDWLT
                     HYELQLEQL"
     gene            4852..5277
                     /locus_tag="VKN64_04685"
     CDS             4852..5277
                     /locus_tag="VKN64_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59570.1"
                     /translation="MNINIILNPAVLRIAGDEQLEGILGEVSVVKGSKKHNKMENPCF
                     TVENRYLEVDPGDDKYQGSFLITFYADDYKTGNADIELLGQVVDMIKEIFNYNPFNLN
                     NYKNYYLEVSNISKAEYNNNFPNQHYMVIEVEYKVVKTG"
     gene            5288..5704
                     /locus_tag="VKN64_04690"
     CDS             5288..5704
                     /locus_tag="VKN64_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59571.1"
                     /translation="MAGNLTNYCEELALDLLFRNTGISPPDTYLGLSTSEILEETLLD
                     DIVEESDANYERKELLFTSPSQSNETAVVKNSNKIVFGPWSADALNPITYAYICDVQI
                     GIEGNILAYFNLPNTRQPAAGESLTILVDDCTLKIG"
     gene            5714..6502
                     /locus_tag="VKN64_04695"
     CDS             5714..6502
                     /locus_tag="VKN64_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HMA59572.1"
                     /translation="MVIKWESVDGKIIEFTNKNKYILEKKTGFSYPPNDLKLVKSPYQ
                     IGSTLIGQVIEPRVIEIEFTIITEDKELLLQRKREVINAFNPLKGKGKLTYDDQDNTY
                     QIKAVSEQSPKFLKNRRTYQDIYLKLHAPDPRWYNPDEFTAVLTQNTEIVNNGDTQTP
                     CIFKLNGPMSNPMIINKTTGQKISINHTTSSGEKVVINTKFGSKSIVLYDSLERKRKA
                     FSLLSFDSQLFGLVRGSNIIYFRASNMNEESNGIINYKERYLRI"
     gene            6522..6929
                     /locus_tag="VKN64_04700"
     CDS             6522..6929
                     /locus_tag="VKN64_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59573.1"
                     /translation="MAGSLTNAGETLALNKVFRDNDDIYVGLATSTINEADDLATIIE
                     EDDANYIRKLITFTSPVDDGTGSFKIENDVLVEFPVFSDDADNNITYAFITKEFTATS
                     GDIIAFADLGQGKQPGAGGKLYIPAGGLEITLD"
     gene            6945..>7563
                     /locus_tag="VKN64_04705"
     CDS             6945..>7563
                     /locus_tag="VKN64_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59574.1"
                     /translation="MALGDIILEYTMGTSRIENLELFDNGKKLAMSDYNDDVIIWDLE
                     KDEIITRVSNTHDLYDFKVSNDGSMVVSNNGGDIKVWDIETGNIITTFTSHSHEVRQL
                     TISNDDTRVISACNNEVYVWEIATNNILTTFTDYEVAIYALDISPDGTKIASGGYNNK
                     IKIWNINTGNIAHTIDDPQDDINSLHFHPDGDKIIACIENHKIKIY"
BASE COUNT         2900 a         1077 c         1640 g         1946 t
ORIGIN      
        1 aactaggagt aatagaggca ggtaactttc agggtttgca cgacacatta gataagtact
       61 tagatttggt aagtataata acaaaggtcc cgcgtataga gctaaacaga atggggagta
      121 gtggtttcgg gaccggcggt gttccgacag gaattgcctt gaggacaata taccagtcat
      181 ttatagggaa gactaatgaa aaatcaaact taataaaatc aggcctggag aacacagcaa
      241 aaaaattatt taagatggct gaagtagata atatagatac aaagtttgaa tatcaagagt
      301 tcaaaacgaa gatacatata agatcaggac ttccttctga tgataaagaa aaaatggaag
      361 ttcaggaaaa agagatcaac aataaaataa aatcaagaga aacagtaatg caggagaggg
      421 gaatagaaga tgtacaaaaa gaaaaagaac tgataaaaaa tgaattaagt gaggatgata
      481 tatacagcga cagaatagca caagaactat cgatagaaga agatatataa tgatatataa
      541 aaaggcagaa attatccaaa aagaatacag gaaacagtat ctaaagaaaa taactgaatt
      601 cgaaacaaat tatgacaaaa tactgtataa atacttagat agaatcagga aaactttctt
      661 tgataactca aatgggagtg gtcagataaa gcagagatca aaaaataaag taaaacttag
      721 tttgaaagtt ttaaacaact ggctggaaat agaaacagaa aatatattag atggctgtat
      781 atcaaaaact ttaaagatag cagtattagg ccagcttaaa acattacaat atgtattaaa
      841 gtatataaat aaaacagata acatatcaaa atatcgcagt aacttacagg ttatcaaaaa
      901 tgaatatagc agtgaaaaaa taaagaaaat taagaattat atctggaaac gggagtggga
      961 agataatcta aatatagatg acaggttgag aaaccttaat aagaaacaca ataaagaaat
     1021 agacgctgta attaattctg atcataaaac tgtatcagca tataatgaaa ttaaaaatac
     1081 aaccacaaat ccggctaagc aaacctataa atataatatt tcaaggctgg ccttaacaga
     1141 aactaacagc ggatataggt atgttcaaaa agagataggc gaaaaatcga atcttgtaga
     1201 ggggataaag tggaatttat cggccagcca ccctaaatat gattatttag agatatgtga
     1261 aagatatgcc ctaaaagatc attcgggttt aggtacagga gtgtataggc cgcaaaatct
     1321 tcccatatta ccgcatccaa attgtttatg ttatctaaca tatttttaca aaaacaatat
     1381 aattattaca catttataac atatttggct gttaaactaa aaatacaaaa ataaaagatg
     1441 gttgataaag actgctttca ctcccagatg aaagtaaaaa cagttaatat aattctattt
     1501 cccccttacc ccgagtgatc ggggtttttc tctgccagca gaatataatg tgaaacagga
     1561 aagtgtccgc aaggacactt ttattgtttt ttcttttttt ctttttcttt tttaattttt
     1621 tccaaaaagg agagtgcaaa tgtggacaag gtattggaaa tcttaaaaaa taatgatata
     1681 gaagtaactg aagaaataaa agagagctta aataatttgg ctgaagaggg taatgaaaca
     1741 aaaacccctt tagatcaaac agaagtaaat aaaattgtca aagaccagtt agaccaggag
     1801 agatctgcac acgaaagtga gatagaaaaa ttaaaaacag agatgaaaga cttagtagat
     1861 cccgagaaag tcgaggagta tgaaagtaag attaaaggcc tagaagagtc aaaggacaga
     1921 ttaaagagcg gactgatcaa ggattacgaa ttgaagatgg ctgctttaaa agagggagtc
     1981 gaggatatcg aatactttga atacttagtt gaaaaaaatc aggttaagga caagctgcgg
     2041 ttaaatgatg acaatattct taatgttacc gatcaggacg gcaatttttt aaccgaaggt
     2101 ggtaaaaagg tcggtgtgga aatgataatt tctgagatga aagaagaaaa accagatctt
     2161 tttaccggtg gaggtaaaaa aaacacatcc ggtccgacca atccagacag cagtgttgac
     2221 cttgatgaat ataggagaga aaacacaaag aagatagcag aagagatggg atatgcaaaa
     2281 taatagggag tggtactttt gactttaaaa ccgagagagt cagaaaaaac tgaatttata
     2341 cagatattaa aaagtaagca tgcgagatat atcgatggaa tggtagttgt agataaagat
     2401 tctgcagttg atgaatatat accacctggt gcagccctgg gtcagattac ctcaagcggt
     2461 aagtatggac ctgttacaag ggataaagtt gatacaggag gagctgatac taccgataat
     2521 ttaataccgt taaaaaactt agataataat aagtggtcta actggcaggt tggtgatgag
     2581 gtaatctgtg acccgggcga aacaaacgaa gagacagctg tgataacatc tatagactgc
     2641 gaaattggag aactaacagt cgacggtatt acggtcgaac acgcagaaga tgtaatcgta
     2701 cagaaaaatg acggtagttc caaagctgaa atagtatgtt tagaattaat cgatgtaagt
     2761 ctagaagatg ctgtgatagg aggtctatta catggggcgg tattttcaga tagaatgcct
     2821 aactatgaca gtatcgtggc agaagaccta ccgcaaatca actttgagta aaggagtagt
     2881 ggctaatgaa taaaaatcag ctgatagaaa taattgaaga caggctaggt atcgattacc
     2941 tgggctttat agaagaaata ggagagccgg agaaattcat cggtaataaa tacctgccgg
     3001 caaaaaatga acatgattat gactgggttt atcacatatt tgacaataca gttgcaatgg
     3061 ccaagatgat ggccagggga gatgcagaaa ctccgatcgt cggcggaccg gctgttaaaa
     3121 aggttgcagg ttcagtcgct cccttcggac agaagtttga agtcaataag tcgatattaa
     3181 ataagatttt caatccgaga aatgataatg aattaaagtc taacttaagg agaatactgg
     3241 atcaatccgc aaggaatgta aggtctgccc aggcgaggag agagtggctg aggtttcagg
     3301 tgttagccaa ggggtcaata accttacagg atagaaatga aaataacgta atttctgtcg
     3361 acttcgggtt acccggcaac cataaaatag attcttcagc cctggaagga gatgcctggg
     3421 atggaagctc tccaaaaccg ttgagtgact tgatcaatgc ctgtgatata tattatgaaa
     3481 caaacgatga aatgccgggt gaaatattgt taagaaaagc acaggccaaa cagatcacgg
     3541 gttcagccga agtagcagga gagttttctg aaaatgctac caggatatca ttaggagaag
     3601 taaatgatta tctcgctgaa ttagggtatc cgccgatcaa agtccacgat gttaaggtca
     3661 atactgaagg gcctaacggc agaccgacca acagcgagta cctgatacct gataataggg
     3721 ttgtccttgt caaaaaagcg ggaggccagg agataaaaga taccggaagg ttagttatgg
     3781 ggccggtttc tgagaataac tttaaacccg gtatatttac gacaatgtat gaagaattag
     3841 acccgatgag gtactggcac tttatgaaag cagaaatgtg gcctgcggtg tataaccccg
     3901 aatatattct tttcatggat gtacaggctt aataatggga gtggtaaaat tatatgatgc
     3961 tggtaaaagc agactggaaa gtaggttacc ctaccggtaa agagagagta atatacagcc
     4021 cggcagataa atttaagtgt gacgaagatt ggggtaaaaa gaaagcggct gccggtaaga
     4081 ttaccgttat caaagaagaa ataataaaca aagagatgtc ggtaaaacag cttaaagata
     4141 aagctaaaaa actttcaatt agcggttact cctcgatgaa aaagaaagaa ttaattgaac
     4201 acataaaggg acatactgat ggataaagtt caaaaactca ggcatttagt atgggatata
     4261 ggtaaaaacg tatttaccga tgatgaacta ctgaaagaat ttcttgaaga ctgcagtggt
     4321 aatgtctata atgctgcagc gcttgtgctt gatgtggtaa gggctgatcc cgagaggatc
     4381 acagaatata ggcgcggtgg ggtgagtgtt tccaagcaga acttagataa agcagtcagc
     4441 aagtatgaag agatcggtgg aagtctaatt atgacagtaa aagaaaagag ggttttcgag
     4501 tgaggatacc ccgcgataaa agttttgttg aggtggaaat agagagatat acaggcggca
     4561 gcacagatct tacttccggc caatggcagg atgctgaatt agtacttgta gctgatgcgg
     4621 agattgatat ccagccgaaa actggtagtg aaacggacag ttttagtggt tcatcttaca
     4681 ataaaaggct ggccggattt ataggaatcg atgatataac tttcagtaca ggattttcaa
     4741 tgataatgca gggagacctt gttgatcaaa aatataaggt ggttgatata aaagattggc
     4801 tgacacatta cgaactgcag ttagaacaac tgtgattaag taggtgtaaa cttgaatata
     4861 aatattattt taaatccggc tgttttgagg atagccggcg atgaacagct ggaaggtata
     4921 ttgggagaag ttagtgtagt taagggatct aaaaagcaca acaagatgga aaacccatgt
     4981 tttacagtag aaaatagata tttagaggta gaccccggtg atgataaata ccaggggtct
     5041 tttttaatta cattttacgc tgatgattat aaaacaggca atgcagatat agagcttttg
     5101 ggacaggtag tagatatgat aaaagaaatc tttaactaca atccttttaa tttaaataat
     5161 tacaaaaact attatttgga agtcagcaat attagtaagg cagagtacaa taataacttt
     5221 ccaaaccagc actacatggt aatagaagta gaatacaagg tagtaaagac agggtaggag
     5281 tgtaaagatg gcaggaaatc tgacaaatta ctgtgaggag ttagcacttg acctcctttt
     5341 tagaaataca ggaatttcac caccagatac ttatttgggc ctctcaacat cagaaatatt
     5401 agaagagact ttattagacg acatagtaga agaaagcgat gcaaattacg aaagaaaaga
     5461 gctgcttttt acttcaccat cacaaagtaa tgaaactgca gtagttaaaa acagcaacaa
     5521 gatagtattc ggaccctgga gcgcagatgc gttaaatcct ataacatatg cttacatctg
     5581 tgacgtacag ataggaattg agggtaatat tttggcttac tttaatcttc cgaacacacg
     5641 gcagcctgca gctggggaat cgctgacaat attggtagat gactgcactt tgaagatagg
     5701 atagggagct tagatggtta taaaatggga atctgtagat ggaaaaataa tagaatttac
     5761 aaataaaaat aaatatatct tagaaaagaa aactgggttt agttacccac ccaatgattt
     5821 aaaattggtg aaatctcctt atcaaattgg cagcacatta attgggcagg tgatagaacc
     5881 gagggttatc gaaatagaat ttacaataat aacagaagac aaagaactat tattacagcg
     5941 aaaaagagaa gtaattaatg cttttaaccc gctcaaggga aagggcaaat tgacatatga
     6001 tgatcaagat aatacttatc agattaaggc agtatccgag caatcaccaa agtttcttaa
     6061 aaacaggaga acttatcaag acatatattt aaaattacat gcccctgatc cccgctggta
     6121 taatccagat gaattcacag ctgtattaac tcagaacacc gagatagtaa acaacggaga
     6181 tactcaaaca ccttgtatat tcaaattaaa tggaccgatg agtaacccta tgatcataaa
     6241 taagacaaca gggcaaaaga ttagtataaa tcatacaacc agtagtggag aaaaggtggt
     6301 tatcaacact aaatttggaa gtaaaagtat tgtcctttat gacagtctcg aaagaaaaag
     6361 aaaagccttc tctctactgt cttttgattc tcaacttttt gggcttgtaa ggggcagtaa
     6421 tattatatat tttagagcat ccaatatgaa cgaagaaagt aatggaataa taaattacaa
     6481 ggaaagatac ctcagaatat aaataaatta ggagtgagaa aatggctggc agtttaacaa
     6541 atgctggaga aaccctggca ttaaataaag tatttagaga taatgatgat atttatgtgg
     6601 ggttagctac ttctacaatt aatgaagctg atgatttagc aaccataata gaagaagatg
     6661 atgcaaatta cataagaaaa ttaattactt ttacatctcc agttgatgat ggtacaggaa
     6721 gctttaaaat agagaatgat gtcctggtag aatttccggt tttcagtgat gatgctgata
     6781 ataacataac ttatgccttt attaccaagg agtttacagc tacttctgga gacataatag
     6841 cctttgcaga tttgggtcag ggcaagcagc cgggagctgg tggaaaactt tatatacctg
     6901 caggtgggtt agagataaca ttagactaaa aggagattta taatatggct ttaggagata
     6961 ttatattaga atatacaatg gggacaagta gaattgagaa ccttgaactt tttgataacg
     7021 ggaaaaaatt agcaatgagt gattataatg atgatgtaat tatatgggat ttagaaaaag
     7081 atgaaataat aacaagagta tcaaatactc atgatttata tgattttaaa gtatctaatg
     7141 atggaagtat ggtagtatcg aataatggtg gtgatattaa agtttgggat atagaaactg
     7201 gaaatatcat tacaaccttt acaagccaca gccatgaagt aaggcaatta actatctcca
     7261 atgatgatac ccgggtcata tctgcctgca ataacgaagt atatgtatgg gagattgcaa
     7321 caaataatat actaactacc tttacagatt atgaagtagc gatctatgct ttagacatat
     7381 caccggacgg aaccaaaata gcatcaggtg gatataataa caagataaag atttggaata
     7441 ttaatacagg aaatattgct catacaattg atgacccaca ggacgatatt aactctctgc
     7501 atttccaccc agatggtgat aaaataatag cctgcattga aaatcataaa ataaaaattt
     7561 atg
//
LOCUS       DATGCJ010000090         8739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_102232, whole genome shotgun sequence.
ACCESSION   DATGCJ010000090 DATGCJ010000000
VERSION     DATGCJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102232"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VKN64_04710"
     CDS             <1..924
                     /locus_tag="VKN64_04710"
                     /inference="COORDINATES: protein motif:HMM:NF021143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HMA59575.1"
                     /translation="GSRDYFDGSIDNAGSYGYYWFSSVDDTDVRFLYFNSSDVLFFPG
                     LRASGLSVRCLRDSGYSSSPTSLNTDYIKIKTNPQAFDLTNLDLTNVTFEHITPFSCG
                     DDITFTYNGTEVTYGTVATSGECWMDRNLGASQVATAYNDSDSYGDLVQWGRPDDGHQ
                     ERTSATTSVLADSDHPGHSNFITNGSSPYDWRDPQNDNMWNTDGSGSNNGETCPEGWR
                     VPSESEWSSVSSNWSNRSDAFNSALKLPSAGTRTCLSASLGNVGSDGNYWSSSVDGTG
                     ARYLFFYSSDVYFHSTNRANGLSVRCLRDSD"
     gene            1380..2636
                     /locus_tag="VKN64_04715"
     CDS             1380..2636
                     /locus_tag="VKN64_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA59576.1"
                     /translation="MDFIKRKLYKDLKKHLSKKEITLLVGARQVGKTTLLLKLKDELE
                     KENTLYISLDEDKEFFTDQKKFLNYLNLELGNSGYVFIDEIQRKENAGLFLKGIYDYV
                     QRKESLNYKFIVSGSGSLELKEKVHESLAGRKRMFELGSVSFKEFVNYRTNYKYENKL
                     KDYFEIKTEEAFNFFKEYFNFGGYPRVVTSSKMKEKREIMKEIIESYLEKDIKNILNL
                     RKEDVFRKLIRILSSQVGSLLNYNRLASEVGVDVKTLKRYLYYGEKTFVLKLLSPFYK
                     NKRKEIVKSPVVYFKDVGFRNYLFGSFGVEREDNKGLIFENFVYTLLFHELQFSDAEI
                     LFWRTQTGAEVDFIVKRGQKKIPIEVKYSELSQPQIGKSLHSFIKKYNPQKALIVNLN
                     FEKEIKEKETIVKFIPFYKLITEKII"
     gene            2729..3352
                     /locus_tag="VKN64_04720"
     CDS             2729..3352
                     /locus_tag="VKN64_04720"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HMA59577.1"
                     /translation="MNNFKMKVIKSSNKIKNCFLNTLFPPKCIGCKKEGSYICKDCEI
                     FISESTLICPICGRESFKGETHKKCKNNSLDGLVTIWDYEGIIGKGLRLVKYKEITDI
                     LNEFIKRAFKIIVRDKERFSSFLKLTNQNTAIIPLTNKKMFNIIAKEVGSYTNSKVIK
                     KEKVKNFENVIIVSDIFNKGEKIKKKAKRLKEKGFKKVWGFALTRTV"
     gene            3492..4094
                     /locus_tag="VKN64_04725"
     CDS             3492..4094
                     /locus_tag="VKN64_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59578.1"
                     /translation="MKKKILIKIIITFFIITAGVFIFYNWTGKTIKKEKNLGWNNSSH
                     LNSWIEEVRGEEAKEKLNSLPEELFTEKYEDEEFNFSFKYPKDFNIGKFKNGEDSKII
                     LVQYPKKKIGVQIIITPLDEKTELTKELINERVPSYTVENPQPVEIDGKKRGLVFKSK
                     NDNFGESREIWFIFRDILYQISSYQSLDPFIKTFFETFQF"
     gene            4164..6221
                     /locus_tag="VKN64_04730"
     CDS             4164..6221
                     /locus_tag="VKN64_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMA59579.1"
                     /translation="MKNHTKLLTILLLIISGLFTASTIYAGTISKPANNLGLIGYWSM
                     DEGRGNIVSDFSGNSNDGTLEGNPQWVDGRHGKALDFDGSNDYVAISNYHYESDNIYT
                     ISVSSWIKANSDGIIASFDRNEYWRFEVGGNAASSGYVGMSFNGDGGQVDGFDSNAQV
                     ADGNWHHVVFTFDNGGAKLYVDGEQENSITSGSYIGTGGTRYGFIGVGSEAESFDGSK
                     GPTDYFPGIIDETRIYNRALSQSEIQDLYNRGLAKITQPDRTDLVAHWKFDEGRSQTT
                     GDSSFNTNSGILQNGPTWTDGRYGQALDFDESGGNGEGEYVDINSLSNLNNQEEITSM
                     AWVKPKPSSDDRAPITRIDRFYFQHYSDDRLATYWYGWNDAGYHYSSQDSVPTGQWTH
                     VAVTWDQESGVIFYINGEKDNQISNSGIGDSTNWLKIGQESSSRRFNGIIDDVRVYDQ
                     ALSEDEIRGIYESTQHKVNVSQNNKLTDGLVGMWSFDGPDLSGSTANDVSGNSNDGTL
                     NGEPQPVIGKIGQALEFDGSDDYVEVTSDASAAGLIPDHITISFWSKRYNGGNEYSCE
                     VVVNNSYYLRMDNDNNIDFRIWTDLDGDSGNFSGGSFNFREWYHVVGVFNGEIMDVYV
                     DGELRTSKNHQGVLDKSSSPLFFSGRTSGGSYIMEGLLDSVRIYSRALSEKEVQRLYR
                     MGR"
     gene            6239..8437
                     /locus_tag="VKN64_04735"
     CDS             6239..8437
                     /locus_tag="VKN64_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMA59580.1"
                     /translation="MKNYTKLLIILFLILGGLFATSFVYAGTISKPSNNLGLVGYWSM
                     DEGRGNTVSDFSGNGNDGTLEGDPQWVDGRHGKALDFDGSSDYVAIDNLYYNSGGEIP
                     YLTVSAWVKIPEGGGSWSILDYDRSEYFTFAEGRLVTDGTVEFDTTSSGGSVDDFGGT
                     TNIEDGNWHHVAAVFDSSDTNDKKIYVDGELDNEKNAYGDGVALGTGVTRYGFIGDGS
                     EASSFDGGRNSLYYQGKVDEIRLYHRALSDSEIQDLYNRGLAKITQPDRTGLVAHWKF
                     DEGRGQTTGDSSFNTNSGTLNLGTSGNTSVANAWTDGRYGQALQLDGSGTVDGTTYGD
                     HVEIPESITRTDNYPDGVTYSIWLKADNDAVDRMALFRGASTIRHIEIYSDSKKFRTE
                     AAKQNGYSFGTGNFPDEIKGKWSHFTIVFANNEANRPVRWYQNGTLFYTGDMTSGDYP
                     DTEYFSFGSIGRSTGNTSYTYAKSFDGTIDDVRIYSKALTEDEIEGLYESTQHKVNVS
                     QNNKLTDGLVGMWSFDGPDLSGSTANDVSGNSNDGTLNGEPQPVIGKIGQALEFDGDY
                     IQLPSSLENELDGNPEATLSMWIKLNSDQNSASNSGIIQLSGYDNSNGNLYYYHDSSR
                     EGGIWLDVFRTNRVFTGDWQPSFPGIVWHNLVVTTSPGTNGWKMYLNGDLKYQTTGQS
                     EVSVKSSLFGGFRVGQNSGGRSLRGKIDNVRIYNRALSQKEIQRLYRIGR"
     gene            8476..>8739
                     /locus_tag="VKN64_04740"
     CDS             8476..>8739
                     /locus_tag="VKN64_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59581.1"
                     /translation="MLYFQILIAGFLGGVVRGIVGYIKHQFSYTRVEFKLLYFLNIMF
                     LSGVVGLIVTAAIHQAGITFFKVGSFSPAIAVIIGYAGGDFIEN"
BASE COUNT         3031 a         1115 c         1831 g         2762 t
ORIGIN      
        1 ggttctcgcg attactttga tggttccatc gacaatgcag gtagctacgg ctactattgg
       61 ttttcgtcgg tagatgatac ggatgtaagg tttttgtact tcaatagcag tgatgtcctt
      121 ttcttccccg gccttcgagc ctccggtctc tctgttcgtt gcctcaggga ttcgggttat
      181 tcttcctctc ctacctccct caatactgac tatattaaaa taaagacaaa ccctcaggct
      241 tttgatctta ctaatcttga tttaactaat gttacttttg aacatataac acctttttcc
      301 tgtggtgatg atataacctt tacctataat ggcacagaag ttacttatgg cacggtagcc
      361 acttccggtg aatgctggat ggatagaaat ctaggtgcct ctcaagtagc aacagcctat
      421 aatgacagcg attcctatgg agatctagtc caatggggac gtcctgatga tggacaccaa
      481 gaaaggacta gtgctacaac ctctgtttta gctgattccg accaccctgg ccatagtaat
      541 tttattacaa atggatcttc cccttatgat tggagagatc cccaaaacga taatatgtgg
      601 aatacggacg gatcgggttc aaacaatggt gaaacttgcc ctgaaggctg gcgggtacca
      661 agtgaaagtg aatggagttc agttagttca aactggtcaa atagaagcga tgcctttaac
      721 tctgctttaa aacttccttc tgctggtact cgcacttgcc ttagtgcttc cctcggcaat
      781 gtaggtagcg acggcaacta ttggtcttcg tcggtagatg gtacgggtgc aaggtacttg
      841 ttcttctata gtagtgatgt ctatttccac agcaccaacc gagccaacgg tctttccgtt
      901 cgttgcctca gggattctga ttagattttt ggcttttgga tttggcctaa aatacaagtt
      961 tcaagcattt taaaacaaaa aaagaataaa ttaaagcaaa aggacgaccc ggccgtccta
     1021 agaataatgg atgattttaa aaagaaagta ataaaaaata gtaataaaat aaaaaaatgc
     1081 tttttagaca ctctatttcc tccaaggtgt gtaggatgca atagagaggg gagttacatt
     1141 tgtgagaatt gtgaattttt tgtttcggag aattctttgg tttgccccga gtgtggagag
     1201 atgagtttta ccggaaaaaa ataaaataat aagaatattc ccagaataac aaggttattt
     1261 aaaggcttga ctcccagaat aacaaggtta taataggaat aaagtaattg ttttaattaa
     1321 ttattatttt ctataaaata ataatttaat gggaataaag taaacaaaaa gtatttttta
     1381 tggattttat taagcgaaaa ctatataaag atttaaagaa acatttgtct aaaaaagaaa
     1441 ttactcttct tgtgggagcg agacaagtag gcaaaacaac tcttctttta aaactaaaag
     1501 atgagttgga aaaagaaaat actttatata taagcttaga tgaagataaa gaattcttca
     1561 cagatcaaaa gaagttttta aattatctta atttagaatt gggaaattcg ggttatgttt
     1621 ttattgatga aattcaaaga aaagaaaatg cagggctttt tttaaaagga atttatgatt
     1681 atgttcaaag gaaagaaagt ttaaattaca agtttattgt ttccggatct ggaagcttag
     1741 agttaaaaga aaaagtgcat gaatccttag caggaagaaa gagaatgttt gagcttggat
     1801 cggtttcatt taaagaattt gttaattata gaacgaatta taaatatgaa aataaattga
     1861 aggattattt tgaaattaaa accgaggagg cttttaattt ctttaaggaa tattttaatt
     1921 ttggaggata tccgagagta gttacaagta gcaagatgaa agaaaaaaga gaaataatga
     1981 aagaaattat tgaaagctat cttgaaaagg atattaaaaa tattttaaat ttaagaaaag
     2041 aagatgtttt tagaaagtta attagaatac tctcttctca agtcggtagt cttttaaatt
     2101 ataatagact agcttcagaa gttggagtag atgttaaaac cttgaagagg tatctttatt
     2161 atggagagaa aacgtttgtt ttaaaacttc tttcgccttt ttataaaaat aagagaaagg
     2221 agattgtaaa atctcccgta gtttatttta aagatgtcgg cttcaggaat tatttgtttg
     2281 gatcattcgg agttgagagg gaagataata agggattaat ctttgaaaat tttgtataca
     2341 ctcttttatt tcatgaatta caattttcag atgcagaaat tcttttttgg cggacccaaa
     2401 cgggagcgga ggtagatttt attgtaaaaa gaggacagaa aaagattcct atcgaggtaa
     2461 aatactccga gctttctcaa cctcaaatag gaaagtctct tcacagtttt attaaaaagt
     2521 ataatcctca aaaagcttta attgtaaatc ttaattttga aaaggaaatt aaagagaaag
     2581 aaacgattgt taaattcatc cctttttata aattaataac ggagaagatt atttagtggg
     2641 aatcttgcct tctgtgaaac ttttatgaac aagatgaaca ctttagtatt aaagtgaaac
     2701 aaaaacacac cccgctgtcc taaggataat gaataatttt aaaatgaaag taataaaaag
     2761 tagtaataaa ataaaaaatt gctttttgaa tactctattt cctccaaaat gtataggatg
     2821 taaaaaagaa ggaagctata tttgtaaaga ttgcgaaatt ttcatctcag aaagcacact
     2881 tatttgtcca atatgtggaa gggaaagttt taaaggagaa actcataaaa aatgtaagaa
     2941 taatagttta gatggacttg ttactatctg ggattatgaa ggaataattg gtaaaggatt
     3001 aagattagtt aaatacaaag aaataactga tattttaaat gaatttataa aaagagcttt
     3061 taaaattata gtaagagaca aagagagatt ttcttctttt ttaaaattaa ctaatcaaaa
     3121 tactgctata atacctctta ctaataaaaa gatgtttaat attattgcaa aagaggtggg
     3181 aagctatact aatagcaaag ttataaaaaa agaaaaagta aaaaactttg aaaatgtaat
     3241 tattgtaagt gatatcttta ataagggaga aaagataaaa aagaaagcta aaagactaaa
     3301 agaaaaaggt tttaaaaaag tatggggttt tgctttaaca agaacagtat aaaaaaatta
     3361 agtttgattt tataaataat aaaaagataa taaagtataa ttatttttag attataaaca
     3421 tcacaacaca tagtttgcag tagtggataa tatagttaaa tagaattaat ttaagagcaa
     3481 aataagatag catgaaaaaa aagatattga taaaaattat tattaccttc tttattatta
     3541 ctgctggagt gtttattttt tataactgga ctgggaagac tattaaaaaa gaaaagaatt
     3601 tggggtggaa taattcctct catcttaact cttggataga ggaagtaagg ggagaagaag
     3661 cgaaagagaa attgaactct ctaccagagg agctgtttac agaaaaatat gaagatgaag
     3721 aatttaattt ttcttttaaa tatccgaagg attttaatat aggaaaattt aaaaatggag
     3781 aggatagtaa aataatatta gtgcaatatc caaagaaaaa aataggagtt caaataatta
     3841 ttactcctct tgatgaaaaa actgaattaa caaaagaatt aattaatgaa agagtgcctt
     3901 cctatacagt agaaaatcct cagccagttg aaatagatgg taaaaaaaga ggattggtat
     3961 ttaaaagtaa aaatgacaat tttggagaga gcagggagat ttggtttata tttagagata
     4021 ttttatatca aattagttcc tatcaatcac tagatccatt tattaaaaca ttctttgaaa
     4081 cttttcagtt ttaaatttct aaattgataa taaatataaa aagaatatag aaaattcaat
     4141 ataaaaaaag agatagataa aagatgaaaa accatacaaa gttactaaca atacttcttt
     4201 taataattag tgggctattt acagcttcta ctatttatgc tggtactatt tccaaacctg
     4261 ctaataatct tggtttaatt gggtattggt ctatggacga aggtagaggg aatatagtta
     4321 gtgatttttc cggtaatagt aatgatggaa ctttggaggg taatcctcag tgggtagatg
     4381 gtagacatgg taaggcactg gattttgatg ggagtaatga ttatgttgct ataagtaact
     4441 atcattatga atcggataat atatatacta tttctgtatc cagctggatt aaagctaatt
     4501 ctgatgggat tatagcgtcc tttgatagaa atgaatattg gcggtttgaa gtaggaggaa
     4561 atgccgcttc tagtggatat gttggaatga gttttaatgg ggacggagga caagtagatg
     4621 gatttgatag caatgctcaa gtagcagatg gaaactggca tcatgtagtt tttacttttg
     4681 ataatggagg cgcaaaactt tatgttgatg gagaacaaga aaattcaata acttcggggt
     4741 cttatatagg aacaggtgga acaagatatg gttttattgg agtaggatca gaggctgagt
     4801 catttgacgg ttctaaagga cctactgatt attttcctgg tattatcgac gaaacccgta
     4861 tctacaaccg agctttaagc caaagtgaaa ttcaagacct ctataatcgt ggtttagcta
     4921 aaattactca acctgataga actgatctag tagctcactg gaaatttgat gaaggccgta
     4981 gtcaaactac tggtgatagt tcttttaata ctaatagtgg gattttgcaa aatggtccta
     5041 cttggactga cggtcgctat ggtcaagcat tagattttga tgaatctgga ggaaatggag
     5101 agggtgaata tgtagatatt aattcactca gtaaccttaa taatcaagaa gagataactt
     5161 caatggcttg ggtaaaacct aaaccatcaa gtgatgatag ggctcctata actagaatag
     5221 atcgtttcta ttttcagcac tatagtgatg atagattagc tacctattgg tatggatgga
     5281 atgatgcggg ttatcattac tcttcacaag atagcgtacc tacaggtcaa tggactcatg
     5341 tagctgttac ttgggatcaa gaaagtggtg ttatttttta tataaatggg gagaaggata
     5401 atcaaattag taattcagga attggagatt ctactaattg gttgaaaata ggtcaagaaa
     5461 gttcaagtag aagatttaat ggaattatag atgatgttcg tgtttatgac caagccctat
     5521 ccgaagacga aataagagga atctatgaga gcactcagca taaagtcaat gtttctcaaa
     5581 ataataaatt aactgatggc ctggtaggca tgtggtcctt tgatggccct gatctttctg
     5641 gttctactgc taatgatgtt tctggtaata gtaatgatgg cactctaaac ggtgaacccc
     5701 aacctgttat tggtaaaata gggcaagcgc ttgagtttga tgggagtgat gattatgtgg
     5761 aagtaacatc tgatgcgagt gcggctggat tgattccgga tcatattaca atatcatttt
     5821 ggtcaaaaag atataatggt gggaatgaat attcttgtga agtagtagta aataattcat
     5881 attatttaag aatggacaat gataataata tagactttag aatatggaca gatttagatg
     5941 gagatagcgg caactttagt gggggcagtt ttaattttag agaatggtat catgtagtag
     6001 gtgtttttaa tggagaaatt atggatgtct atgtcgatgg agaattaaga acttccaaaa
     6061 atcatcaagg agttttggat aaatcttcaa gtcctctttt tttcagtgga agaacaagcg
     6121 gaggtagtta tattatggaa ggattattag atagtgttcg gatctacagc cgcgctctaa
     6181 gcgagaagga agttcaaaga ttatatagaa tgggaagatg aagctacaaa ttaaaaaaat
     6241 gaaaaattat acaaagttac taataatact ttttttaata cttggcggac tatttgctac
     6301 ttcttttgtc tatgctggta ctatttctaa accttccaat aatcttggct tagttgggta
     6361 ttggtctatg gacgaaggta gaggtaatac agttagtgat ttttccggaa atggtaatga
     6421 tgggactttg gagggtgatc ctcagtgggt agatggtaga catgggaagg ctttggattt
     6481 tgatggtagt agtgactatg tagcgattga taatttatac tataattctg gaggcgagat
     6541 accatatctg actgtttcgg catgggttaa aatacccgaa gggggaggga gttggtctat
     6601 attggattat gatagaagtg agtattttac atttgcagaa ggacgactgg ttacggatgg
     6661 gacagtagaa tttgatacca ctagctctgg cggatcggta gacgattttg gaggtacaac
     6721 taatattgag gacggaaatt ggcatcatgt agcagcagta tttgatagtt cggacactaa
     6781 tgataaaaaa atttatgtag atggagagtt agataatgaa aaaaacgcat atggcgatgg
     6841 ggttgcttta ggtacagggg ttaccagata tggttttatt ggagatggtt ccgaagcttc
     6901 atcttttgat ggtggtagaa acagcctgta ttaccaaggg aaagttgatg aaattcgctt
     6961 atatcatcgt gctctctccg actccgaaat tcaagatctc tataatcgtg gcttagctaa
     7021 aataactcaa cccgacagga ctggtctagt agctcactgg aaatttgatg aaggccgtgg
     7081 tcaaactact ggtgatagtt cttttaatac taatagtggg actcttaatt tgggaacttc
     7141 aggtaatact tctgttgcta atgcttggac cgatggtcgt tatggtcagg cattacagct
     7201 tgatggaagt ggaacagtgg atggaacaac ttatggagat catgtagaaa taccggagag
     7261 cattactaga actgacaatt atccagatgg tgttacttat agtatttggt taaaagcaga
     7321 caatgatgct gtagatagaa tggctttgtt tagaggagct tctacaatta gacatattga
     7381 gatatatagt gattcaaaaa aatttagaac agaagctgct aaacagaatg gatatagttt
     7441 cggaacgggt aatttccctg atgagataaa aggtaaatgg agtcatttca caattgtgtt
     7501 cgctaacaat gaagctaata ggccggtaag atggtatcaa aacggaacat tgttttatac
     7561 gggagatatg actagtggtg actatccaga cactgaatac tttagttttg gaagcatagg
     7621 tagatcaact gggaatacta gttatactta tgcaaaaagt tttgacggca ccattgatga
     7681 tgtccgcatc tatagtaagg ccttgaccga agatgaaata gaaggactct atgagagtac
     7741 tcagcataaa gtcaatgttt ctcaaaataa taaattaacc gatggtctgg taggcatgtg
     7801 gtcctttgat ggccctgacc tttctggttc tactgctaat gatgtttctg gtaatagtaa
     7861 tgatggcact ctaaacggtg aaccccaacc tgttattggt aaaatagggc aagcgcttga
     7921 gtttgatgga gattatattc aactaccctc ttctttagaa aatgaacttg atggaaatcc
     7981 tgaagctaca ttaagtatgt ggataaaact aaattctgat caaaatagtg catctaattc
     8041 cggaataata caattaagtg gatatgataa tagtaatgga aatctttatt attatcatga
     8101 ttcttcaaga gagggaggta tttggttaga tgtctttaga acaaataggg tttttactgg
     8161 tgattggcag ccctcctttc caggaatagt gtggcataat ttagttgtaa caacaagtcc
     8221 aggtactaac ggttggaaga tgtatttaaa tggggattta aaatatcaaa ccacaggaca
     8281 atctgaagtt tctgtaaaat cttctttatt tggggggttt agagtgggtc aaaacagtgg
     8341 ggggaggtct ttgagaggta aaatcgataa cgttcgcatc tataaccgtg ctttaagcca
     8401 aaaagaaatt caaagattat ataggatagg gagataatgt aaaattattt attattcatt
     8461 aaatattaaa aatttatgct ttattttcaa attttaatag caggtttttt aggaggagta
     8521 gtaagaggga tagttggcta tattaaacat caattttctt atactagggt agaatttaaa
     8581 ttgctttact ttttaaatat tatgttttta tctggagtag tagggcttat tgttactgca
     8641 gctatccacc aggctggaat tacttttttt aaagtaggct ctttttctcc tgcaatcgct
     8701 gttataattg gttatgctgg aggtgatttt atagaaaat
//
LOCUS       DATGCJ010000091        12275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_414078, whole genome shotgun sequence.
ACCESSION   DATGCJ010000091 DATGCJ010000000
VERSION     DATGCJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414078"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1162
                     /gene="ftsZ"
                     /locus_tag="VKN64_04745"
     CDS             83..1162
                     /gene="ftsZ"
                     /locus_tag="VKN64_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMA59582.1"
                     /translation="MFDLGTEMEQFANIKVIGVGGGGNNALNRMIEEGLEGVDFTAIN
                     TDAQALMSSNASMTIRIGQSITKGLGAGAHPDIGREAAKENIEEIREAVKGADMVFIT
                     AGMGGGTGTGASPIIAQAAKEMGALTVGVVTKPFTVEGKKRMDNAVEGIKELKDSVDT
                     LIVIPNDRLLDVAEPNTSLIDAFKIADNVLRQGVQGISDLITITGIINLDFADVKTIM
                     KDAGTALMGIGMASDEGRAQNAAKKAIESPLLEASIEGAQGVLINITGGNDLGIHEAN
                     EAAKVIQEVSDPDANIILGAVIDEEYQDEVKVTVIATGFDREATQKPRKQQTQQEQDE
                     DLETDFDMDNFEGDELDIPAFLRKK"
     gene            1247..2056
                     /gene="pgeF"
                     /locus_tag="VKN64_04750"
     CDS             1247..2056
                     /gene="pgeF"
                     /locus_tag="VKN64_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HMA59583.1"
                     /translation="MFTKKVKEGLTYLEFERLKQYGFEVVFTTRLGGFSIPPYESLNL
                     GLHTDDKKEHVLKNRAKVYDTLKFNPDNIIYAEQIHSNDIRLVTTDDKGKGIRDHNNS
                     IKNMDGLIATKKDMILGGHFADCVPIYIMDKDKGYFSLIHSGWKGTYQEILTKTLNFF
                     KDSLASKDEDLLVVLGPSISKNNYEVGFDLINKFKEKFAFPPSYFEQIDNNYFLDLKE
                     LNRLIALENGILNKNLFVSKLCTYKNKNLFYSYRRDQGKTGRMAAFISLLS"
     gene            2067..3002
                     /locus_tag="VKN64_04755"
     CDS             2067..3002
                     /locus_tag="VKN64_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMA59584.1"
                     /translation="MKKDKDTSENRRKFISWKYFVFIVVIIIGLTSFFIFKDKNLKPL
                     TVDYKEIVAGFETEALVIRNEKTYKAPLSGELTILLNEGERAPYGEKIAYIENGEIKY
                     NIYTEQPGIISYAYDGLEERLKFGKITPQVLSEYDQFERDYHQYVSGNKVQKGDKLYR
                     NINNYEQYLLIEVDKERAEKFNQNEVVFVDHNQNNKDGKLIKSKIKKIYKHNNKYFLL
                     VLLDKYLEMWNNTRWVNIKLIKNIYRGLAVPSSAVFKTTAGSQVLLYTFDHEVVLKDV
                     EIVESTEDWTIVENLEIGDQVITNPQNANYGRNDN"
     gene            3004..3702
                     /locus_tag="VKN64_04760"
     CDS             3004..3702
                     /locus_tag="VKN64_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMA59585.1"
                     /translation="MSVNIEKNLKKVYDNIEKNAEKANRDPSEIQLVAVGKNHPPKKI
                     ISAMKAGHYVFGENRVQELLDKHELFFDQEIKWHFIGHLQKNKVKYLMKIKQLDMIES
                     IDSIEIAKAVNKEAKEAERNIKVLLQVNIAEDPNKFGFSKENITEAIDEVSQLDNIIL
                     AGLMTITPYYNEAEDARDDYKKMKQLLDELNQKSYNLQELSMGMSNDYHVAVQEGATI
                     IRVGTAIFGKRKYY"
     gene            3735..4022
                     /locus_tag="VKN64_04765"
     CDS             3735..4022
                     /locus_tag="VKN64_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HMA59586.1"
                     /translation="MSMLIWLISTVFTVLNWLIIFRVIISWVRPDVNNSQWRKFLRIV
                     YNITEPILAPIRRLIPTGSIGIDFSPLVAIFALQIIRNFVISLLRSMMFGI"
     gene            4043..4831
                     /locus_tag="VKN64_04770"
     CDS             4043..4831
                     /locus_tag="VKN64_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030095 - chloroplast photosystem II
                     [Evidence IEA];
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosystem II S4 domain protein"
                     /protein_id="HMA59587.1"
                     /translation="MFDKEKLLSHLHREDDRKLGDFILDKIEKVLERKSIERTNFLNP
                     YQRKVALGLIEQINGVNHLTEGGYKKAERQRIVIFPDYLFPDHVESSIKVIKVEGNFN
                     FKNITHKDFLGAILGLGIKREMVGDILVLDEFAQIIIAEEIYDFVDLHFKKVNEVPIE
                     ISKITKDDIVIPTQNTKDIPTTVASLRLDAVASAGFGDSRSKISRDIKNKRVKLNFKV
                     VTDPASEVEVGDLISIRNRGRVEVAERRGLSNRNRIKLLLKRYT"
     gene            4872..5378
                     /locus_tag="VKN64_04775"
     CDS             4872..5378
                     /locus_tag="VKN64_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HMA59588.1"
                     /translation="MKINPLDIYNKEFTVSALGYNKSQVEEFLDDLGVEYERMMQEMK
                     DLREKNEKLQEKLKSYENIEENLKNTLKSIEEIADKQTQQAREEANMIIEKANMEAKK
                     MRQEVKKDLAEEYKKLEKLKETKTFFKIRLRNLLESQLEVLEDEEELDLDKYQEEIDE
                     LPETAELD"
     misc_binding    5416..5612
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5667..8456
                     /gene="ileS"
                     /locus_tag="VKN64_04780"
     CDS             5667..8456
                     /gene="ileS"
                     /locus_tag="VKN64_04780"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HMA59589.1"
                     /translation="MSYKETINLPDSDFPMRANLSERELKFQKIWEENEVYERAIENR
                     KGKEPFILHDGPPYANGDIHIGHALNKVLKDIVTRFSTLRGYYSPYVPGWDTHGLPIE
                     HKVTKEMGDKAKELSVAELREKCKEYALKYVGRQREQFKRLGIWGEWDNPYITLNPEY
                     EVKQIEVFGEMAKKGLFYKGKKPVHWCPSCETALAEAEVEYHTDRTPSIYVKFPFAEE
                     VEVGGVNLSPADSYMVIWTTTPWTIPSNLAITLHPDSDYVVVEVAGDKLVMAEKLVDE
                     VMDKVGIEEYEVISETFKGTELENVNTNHPFMDRKSLIILGDHVTLDEGTGCVHTAPG
                     HGHDDFIVGQEYDLEVYAPMDSRGYFTEEAEDFEGLHYDEANIKVTDKLKEKDLLMEL
                     NFIDHQYPHCWRCKGPLVFRATDQWFASIEDIKEETLQAIHDVKWFPSWGEKRMENMV
                     AERTDWCISRQKKWGVPIPIFYCDDCGESIINDETLEAVKEMFAKEGSNSWYENEAAD
                     ILPDGYKCPNCASSNLSKEEDIMDVWFDSGSSHKAVLELRDNLNWPAQVYLEGTDQYR
                     GWFNSSLLTAVATEGKAPYEEVVTNGFTVDEKGNKMSKSQGNVISPHEIIDQYGADIL
                     RLWVASANFKDDVRVSNKILKQNSEVYRRIRNTFRFILGNTNDFVKEENYVEFEDRHE
                     IDRWIGIKVKELAEKVTKSYRNYEYHKVYHDVHNFCTVEMSSLYMDIVKDRLYTDGTE
                     SHSRRSAQSALYDIMMTLVKIISPVLVHTAEEVWQEALDRAEIADSVFLTDWPEFDEK
                     YEDKAFMDKWDELLNIRKDVSKALEIARNDKIIGNSLEARVEISSLNDNVNELLEEEY
                     EKLADFFIVSQVELKHELDEVTHVGENTQIKVYVDEAYGEKCERCWKYSTTVGEDDQY
                     EDVCHRCATVLKEEQ"
     gene            complement(8502..9860)
                     /locus_tag="VKN64_04785"
     CDS             complement(8502..9860)
                     /locus_tag="VKN64_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMA59590.1"
                     /translation="MELRLTENSKIRAKKIFILALPAILEMSLNTMLGVADTLMISRI
                     IGKEGLAAVGFANQIFFTLIFIFSSFNAGATAMIARSYGERNYKRLNKIMGQNLTLNT
                     SIGLVVFFLTFIFAGNILSVYDITPQVKSLGVTYLKIVAYSQLFMFISFAMAASLRGA
                     GDTKTPMYITGSANILNIIGNYVLMTGFWIFPDMGIAGAALSTTISRILASIAYIFIV
                     FNIKEKLQLKFENLKLTKEILQPLWNFSYAAGLEQLSMQLAFFLNGIIVSMLTTTSEA
                     AFRILINIESISFMPAIGTSIATATLVGKHLGENRPEESLKTGFTAAGMGVIWGLFMG
                     IIFLLFPEPIIRIFTTETVVVEASLKTLRIAGINQAPLAFMIIMAGALRGTGDTKGVM
                     IITAMRLWLMFVPLTYLFTILLQQGVTGVWYAEISSFVVFSFVIFKRFKAMEWTEIRM
                     FA"
     gene            10058..10972
                     /locus_tag="VKN64_04790"
     CDS             10058..10972
                     /locus_tag="VKN64_04790"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMA59591.1"
                     /translation="MKEIEITVENTYEGMRLDKYISKIKKDFSRNQIQSLIDKNLVEV
                     NDNLEKKSYQVKTGDEIVIYKEEPKVLDLPKKEMDLDIIYEDEDIILINKPVGLIVHP
                     VKGNRTDTLVNALLAHTEKLGNINGTLRPGIVHRLDKNTSGVILVAKNQQSLNNLQKQ
                     FKERKTKKIYRTILKGTLPYNSGTIDAPIGRNPKNRTKMAVIKENSKKAVTEFKVMKR
                     YNNYTYVEADLKTGRTHQIRVHFAHLGFHIIGDQKYGSKEEKFALKHQLLHAYKIGFY
                     HPQSNEWVEYKADLPEHFQNILKKLKNL"
     gene            10992..12176
                     /locus_tag="VKN64_04795"
     CDS             10992..12176
                     /locus_tag="VKN64_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HMA59592.1"
                     /translation="MSYSNKLSDNVYWVGVNDRETDFFENLWPLPYGISYNSYLIVDE
                     KVVLVDTVKIDYQERYLEKLMDLTDGRPIDYLVINHMEPDHSGSIKELMRLFPDMKII
                     GNNKTTGFLKGFYGITENIKEVDNEETLDIGEKTLKFFITPMVHWPETMMTYLEEENI
                     LFSGDVFGAFGAINQSIFDDEIETDFFEDEIIRYYSNIVGKYSPIAAKHIGSLGDLEI
                     DTVASTHGPVWRENPDYIINKYYDMAKFKADKGAVVIYGTMYGNTKKIAEEAAAGLSA
                     AGVKDVKLYDASRTHKSYLISDIWKYKGVIFASCSYNNELFPPVNSLVEALENRGIQN
                     RYFGFIGTYSWSGGSLERMNQFAEDCNIEVVEPKINNQYAPTADNLDKAFKLGKNVGE
                     KI"
BASE COUNT         4558 a         1777 c         2477 g         3463 t
ORIGIN      
        1 aaaagaaaaa gaaataacag aatctttctt ttcaaaaatt aaaagttggt tccaagattt
       61 cttttaaaag gaggtatata acttgtttga cttaggaaca gaaatggaac agtttgctaa
      121 tataaaagtt atcggagttg gcggcggtgg taacaacgcc ttaaatagaa tgattgaaga
      181 aggattagaa ggggtagatt ttacagcaat caatactgat gcacaagcct tgatgtcttc
      241 taatgcttcg atgaccatcc gtatcggcca gagcattaca aaagggttag gagcaggcgc
      301 tcatccagat attggtagag aagctgctaa agaaaatatt gaagaaattc gggaagcagt
      361 caaaggagct gacatggtat ttataactgc cgggatgggg ggcggaaccg gaacaggagc
      421 ctcaccaatc attgcccagg cagctaaaga gatgggggcc ttaacggtcg gtgtagtaac
      481 caaacccttt actgtagaag gcaaaaagcg aatggataat gcggttgaag gtataaaaga
      541 attaaaagat agtgtagata ccttgattgt aataccaaat gatagattgc ttgatgtggc
      601 agaacccaat accagtttaa tagatgcctt taaaatagca gataatgtat tgagacaggg
      661 agtccagggg atttctgatc taatcacaat aactggaatt atcaaccttg acttcgctga
      721 tgtcaagacg atcatgaaag atgccgggac cgctttaatg ggcattggaa tggccagtga
      781 cgaaggtcgg gcccaaaatg cagctaaaaa agcaattgag tctcctctct tagaagcttc
      841 tatcgaaggt gcacaagggg tattgatcaa cataacagga ggcaatgacc tgggtataca
      901 tgaagctaat gaagcggcaa aagtaattca ggaagtctct gacccggatg caaacataat
      961 attaggggca gttattgacg aagagtacca ggatgaagtg aaagtaaccg taatagctac
     1021 cggatttgac cgggaagcta cccaaaagcc gcggaaacag cagacccaac aggagcaaga
     1081 tgaagattta gaaaccgatt ttgatatgga taactttgaa ggtgatgaac tggatattcc
     1141 cgccttctta agaaaaaaat agatttgaat aaattctgct ataaatataa aagtctcggt
     1201 gtatccacat cgggattttt atatttaatt aattaaaggt gatgagatgt ttactaaaaa
     1261 agttaaagag ggtttaacat atttagagtt cgaaaggctt aaacagtatg gttttgaagt
     1321 agtatttact acaaggctcg gtggtttttc catacctcct tatgaaagtt taaacttagg
     1381 acttcatacc gatgataaaa aagagcatgt attaaaaaac agagcaaaag tatatgatac
     1441 tttaaaattt aatcccgata atattattta cgccgaacag attcactcca atgatattag
     1501 attagtaact acagatgata aaggaaaagg aattagagat cataataatt ctataaaaaa
     1561 tatggatggt ttaatagcaa ctaaaaaaga catgatttta ggtggacatt ttgcggattg
     1621 tgttcccata tatataatgg ataaggataa agggtatttt tctctgattc attccggctg
     1681 gaagggaact tatcaagaaa tattaacaaa aacccttaac ttttttaagg atagccttgc
     1741 cagtaaagat gaggatttac tggtagtatt agggccttca atttcgaaaa ataattatga
     1801 agtaggtttt gatttgataa ataagtttaa agaaaaattt gcttttcctc cctcatattt
     1861 tgagcaaata gataataatt attttttaga tttaaaggaa ttaaatagat taattgcctt
     1921 agaaaacggt atattaaata aaaatttatt tgtttctaaa ttatgtactt ataaaaacaa
     1981 gaatttgttt tactcttacc gcagagacca gggtaaaaca ggcagaatgg cagctttcat
     2041 atctctttta tcataaaggt gattaaatga aaaaagataa agataccagt gaaaatagaa
     2101 gaaaatttat aagttggaaa tactttgtat ttatagtagt tattattata ggattaactt
     2161 cattttttat ctttaaggat aaaaatttaa agcccttaac cgttgattat aaagaaattg
     2221 tggccggttt tgaaactgaa gccctggtta taagaaatga aaaaacttat aaagcgccgc
     2281 taagtggaga attaactatt ttgctcaatg aaggggagag agccccttat ggtgaaaaga
     2341 tagcctatat agaaaatggt gaaattaaat ataatatata tacagaacag cctggtatca
     2401 ttagctatgc ttatgatggt ttagaagaaa gattaaagtt tggcaagata actccgcagg
     2461 tcttatctga gtatgaccaa tttgaaagag attaccatca atatgtttcc ggtaacaagg
     2521 tacaaaaagg tgacaaactt tatcgtaata tcaacaatta tgaacagtat ttattgattg
     2581 aggtggacaa agaaagagcc gaaaaattta atcaaaatga agttgttttt gttgaccaca
     2641 accagaataa taaagatggt aaattaataa aatccaagat taaaaagata tataaacata
     2701 ataataaata ttttttatta gtacttctag ataagtattt agaaatgtgg aataatacaa
     2761 gatgggtgaa tattaaacta atcaaaaata tttatagggg tttagctgta ccttcttctg
     2821 ctgtctttaa aacaacagct ggtagtcaag tattacttta tacttttgac catgaagtag
     2881 tcttaaagga tgttgaaata gttgagagta ctgaagactg gacgatagtc gaaaacttag
     2941 agattggaga tcaggtaata accaatccac aaaatgcaaa ctacggaagg aatgataatt
     3001 aagatgtcag taaatatcga aaaaaatctt aaaaaagtat atgataatat tgaaaaaaat
     3061 gctgaaaaag caaatagaga tccttctgaa atacagttag tagcggttgg aaaaaatcat
     3121 ccccccaaaa agataataag tgctatgaaa gccgggcatt atgtatttgg ggaaaacagg
     3181 gttcaggaac ttttggataa acacgaatta tttttcgacc aggaaataaa gtggcacttt
     3241 attggacatc tacaaaaaaa taaggtaaaa tacttaatga agattaaaca attagatatg
     3301 atagaatcta ttgatagtat tgagattgcc aaagctgtta ataaagaagc taaagaagct
     3361 gaaagaaata tcaaagtact tttacaggta aatattgcag aggaccccaa taaatttggc
     3421 ttttcaaaag aaaatataac tgaggcaata gatgaagtct cccaattaga taatattatt
     3481 cttgcgggct taatgaccat tacaccttat tacaatgaag cagaagatgc ccgagatgat
     3541 tataaaaaaa tgaagcaatt attagatgaa ttgaaccaaa aaagctataa tttacaagaa
     3601 ctatcaatgg gcatgtcaaa tgattatcat gttgcagtcc aggaaggcgc aactattata
     3661 agagtcggaa ctgcaatatt tgggaagaga aaatattatt aaatatagat agagaaacaa
     3721 aaggagctgt tataatgagt atgttaatat ggttaataag tacagtattt acagtattaa
     3781 actggcttat tatatttcgt gtaataattt cctgggtaag accggatgtc aataatagcc
     3841 aatggagaaa atttttaaga atagtatata atattactga acctatatta gctcccatac
     3901 gtaggttaat accaactgga agtattggga tagatttttc tcctctagta gctatttttg
     3961 ccttgcagat tataagaaac tttgtaatca gtctactgag gagtatgatg tttggaattt
     4021 aattaagagg tgaatgcagt atatgtttga taaagaaaaa ctactttccc atcttcaccg
     4081 tgaagatgat cggaaactag gagattttat actagataag atagaaaaag tattagagag
     4141 aaaatcgata gaaaggacca acttcttaaa cccttaccaa agaaaagtgg ctttggggtt
     4201 gattgaacaa ataaatggag ttaatcattt aacagaaggt ggttataaaa aagcggaaag
     4261 acagagaatc gtaattttcc ccgattatct ttttcccgat catgtagaat cttcaataaa
     4321 ggtaattaaa gttgagggaa atttcaattt taaaaatatt actcataaag attttctggg
     4381 agctatttta ggattgggca tcaaaagaga gatggtcggc gatattttag tattagatga
     4441 atttgcccaa atcataattg cagaggaaat ttatgatttt gttgatctgc attttaaaaa
     4501 ggttaatgag gtccctattg aaataagtaa aattactaaa gatgatatag tgatccctac
     4561 ccaaaataca aaggatatcc cgactacggt tgcctcgttg aggctagatg cagtggcgag
     4621 tgcaggtttt ggagacagcc gcagtaaaat ttcgcgggat attaaaaata aacgagtaaa
     4681 gttaaatttc aaggtcgtga cagaccctgc cagtgaagta gaggtcggtg atcttatttc
     4741 gatccgtaat aggggtcgag tagaagttgc cgaaaggaga ggcctttcaa atagaaatag
     4801 aattaaatta ttattgaaaa ggtacactta aagataaaaa aatccaaata atattaaaag
     4861 gaggagcaag tatgaaaata aacccgctag atatttataa taaagaattt acagtatctg
     4921 ctttgggtta taataaaagt caggtagaag aatttcttga tgatctagga gtagagtatg
     4981 aaagaatgat gcaggagatg aaagacctgc gtgaaaaaaa tgaaaagtta caggaaaagc
     5041 taaaaagcta tgaaaacatt gaagagaacc tgaaaaacac tttgaaaagt attgaagaaa
     5101 tagctgacaa acaaacccaa caagcacgtg aagaagctaa tatgataatt gaaaaagcaa
     5161 acatggaagc caaaaagatg agacaggaag ttaaaaaaga cctagcagaa gaatacaaaa
     5221 aactagaaaa attaaaagaa acaaagactt tctttaagat tagattacgt aatcttttag
     5281 aaagccagtt agaggtttta gaagatgaag aagaactaga tttagataaa tatcaagaag
     5341 aaatcgatga acttcccgaa acagcagaac ttgactaagt ctaataaata atttataata
     5401 taagaaaatt aaaatttaaa agttcagacg gagacagtag ttactattat attaatttga
     5461 gcgagccggg ctggtgggag ccggtaatcc ctaatagtaa tgaagatcac tcttgagccg
     5521 gttaattgaa tgaagtagat taactcgtat gatctgcgtt aaagaatttt aagttaaaat
     5581 tagggtggta ccgcggtctt ttcgcccctt gcaagggtgg aatggccttt ttttattgca
     5641 ataaatcttt aggaggtttt aataaaatga gttataagga aactattaat ctaccagatt
     5701 ctgactttcc gatgagagct aatttaagcg aaagagaact gaaatttcaa aaaatatggg
     5761 aagaaaatga agtctatgaa agagctatcg aaaacagaaa aggaaaagag ccgtttattt
     5821 tacatgacgg cccaccttat gcaaatggag atatacatat cggacatgcc ttaaataagg
     5881 ttttaaaaga tattgttact cgtttttcta ctttaagagg ctattattct ccctatgtac
     5941 cggggtggga tacacatgga ctaccaattg agcataaagt taccaaagaa atgggcgaca
     6001 aagccaaaga attatcggtg gctgagctaa gagagaaatg taaagaatat gctttaaaat
     6061 atgtaggacg tcaaagagag cagtttaaac gccttggtat ctggggagaa tgggataatc
     6121 cctatatcac attaaatccc gaatatgaag tcaaacagat tgaagtattc ggtgagatgg
     6181 ccaaaaaggg attattctat aaaggcaaaa agccggtcca ctggtgcccc agctgtgaaa
     6241 cagccctggc tgaagctgag gttgagtacc atactgaccg gacaccttcc atttatgtga
     6301 agtttccctt tgcggaagaa gttgaagtcg gaggcgtaaa tttaagccca gccgatagct
     6361 atatggtaat ctggactaca actccctgga ctataccttc taacttagcc ataacactgc
     6421 atcccgattc tgattatgta gttgttgaag ttgcgggaga taaattagta atggccgaaa
     6481 agctagtgga tgaagtaatg gacaaggtgg gtatcgaaga atatgaagtg ataagtgaaa
     6541 cctttaaagg tactgagtta gaaaatgtta atacaaacca tccctttatg gatagaaaat
     6601 ctctcataat cttgggcgat catgtcaccc ttgatgaagg tacaggctgt gtgcatactg
     6661 cacccggtca cggtcatgat gactttattg ttgggcagga atatgaccta gaagtttatg
     6721 ctcccatgga ttctagaggt tactttacgg aagaagctga agattttgaa gggttacatt
     6781 atgatgaagc aaatattaaa gttacagata aattaaaaga aaaagatctg ttgatggaac
     6841 ttaatttcat tgatcatcag tatccacact gctggagatg taagggcccg ttagtcttta
     6901 gagcgaccga tcagtggttt gcttccatcg aagatataaa agaagagacc ctgcaggcta
     6961 ttcatgatgt taaatggttc ccctcctggg gagaaaagag aatggaaaac atggtagccg
     7021 aaaggacgga ctggtgtatc tcccgacaga aaaaatgggg agttccgatt ccgatcttct
     7081 actgtgatga ttgtggcgaa tccatcataa atgatgaaac tttagaggca gttaaagaaa
     7141 tgtttgccaa agaagggtca aactcctggt atgaaaatga agcagccgat atattaccgg
     7201 atggttataa atgtccgaac tgtgctagca gtaacctttc caaagaagaa gatatcatgg
     7261 atgtctggtt tgattccggt tcatcccata aagcagtcct tgaattaaga gataatctaa
     7321 actggccagc ccaggtttac ctggaaggta cagaccagta tagaggttgg tttaattcct
     7381 cattactgac tgcagttgcg acagagggta aagctcctta tgaagaggta gttaccaatg
     7441 gatttaccgt cgatgaaaaa ggtaacaaga tgtccaagtc ccaggggaat gtaatttcac
     7501 cccatgagat catcgatcaa tatggagcag atatcttgag attatgggtg gcatcggcta
     7561 actttaagga tgatgtaagg gtatcaaaca agatattaaa gcagaattcg gaagtatatc
     7621 gaagaattag aaacaccttt agatttatcc tcggcaatac caatgatttt gttaaagagg
     7681 aaaattatgt agagtttgaa gatagacatg aaattgatag atggatcggt attaaggtaa
     7741 aagaactggc agaaaaagtt actaaatcat accgcaacta tgaataccat aaagtatacc
     7801 acgatgtgca taacttctgt acagtcgaga tgagttcctt gtatatggat atcgtaaaag
     7861 atagactata taccgatgga actgaatcac acagcagaag gtcagcccaa agtgctttat
     7921 atgatataat gatgacctta gtaaagatta tatccccggt cttagtccat acagccgaag
     7981 aagtatggca ggaggcctta gatagggctg aaatcgctga cagtgtcttc ttaaccgatt
     8041 ggcctgaatt tgatgaaaaa tatgaagata aagcctttat ggataaatgg gatgaattat
     8101 tgaatatcag aaaagatgtt tcaaaagcac tagaaattgc cagaaatgat aaaataatag
     8161 ggaactcttt agaagcaagg gtagaaatca gttctttaaa tgataatgta aatgaattat
     8221 tagaagagga gtatgaaaaa ctggctgatt tcttcattgt ctcccaggtt gagttaaaac
     8281 atgagttaga tgaagtcacc catgtaggtg aaaacacaca aatcaaggta tatgtagatg
     8341 aagcctatgg agaaaaatgt gaaagatgct ggaagtacag tacaaccgta ggagaagatg
     8401 atcagtatga agatgtctgt catagatgtg caaccgttct aaaagaagaa caataataaa
     8461 aagcaaaaag aggggaggac tcccctcttt ttttatttgt attaagcaaa cattctaatt
     8521 tcagtccatt ccattgcttt aaatctttta aatatcacaa atgagaaaac aacaaaagaa
     8581 cttatttctg cataccagac cccagttacc ccctgctgca ataatattgt aaagaggtaa
     8641 gttaagggca cgaacataag ccagagccgc atcgcagtaa taatcatgac tcctttagta
     8701 tcaccggtac cccttaatgc ccctgccatg ataatcataa aggctaaggg ggcctgatta
     8761 atgcccgcga tccgtaaggt ttttaaagat gcttcgacca ctaccgtttc ggttgtaaag
     8821 attcgaataa ttggttctgg aaataacagg aagataatcc ccataaaaag tccccagata
     8881 actcccatcc cggccgcggt aaagcctgtt tttaaagatt cttccggtct attttcaccc
     8941 agatgtttac caactaaggt agcagtggca atagatgttc cgatagccgg cataaaagag
     9001 attgactcta tatttattag gatccgaaaa gcagcttctg aggtcgttgt taacattgat
     9061 acaattattc cgtttagaaa gaaagctaac tgcatcgata gctgttctag acccgccgca
     9121 taactaaagt tccaaagcgg ctgcaatatt tcttttgtta gttttagatt ttcgaatttc
     9181 aactgtaatt tttccttaat attgaaaacg ataaaaatat aggcgattga ggctagaatt
     9241 ctagaaatag ttgttgatag agcggcccct gcgattccca tatcagggaa gatccagaaa
     9301 ccggtcatta atacataatt acctataata tttagtatat tagctgaacc agtgatatac
     9361 atcggtgttt tcgtatcacc agcacctctt aaactggcag ccattgcaaa tgaaataaac
     9421 ataaatagct gactgtaagc tacaatcttt aagtaggtta cccctaagga cttaacctgg
     9481 ggagtaatat cataaacact taaaatattg ccggcgaaaa taaatgtcaa gaaaaatact
     9541 accagaccaa tagaggtatt taaggtgagg ttctgtccca ttattttatt taatctttta
     9601 taatttcttt caccataact tctagctatc attgcggtag ctccggcatt aaaagaagaa
     9661 aatatgaaga tcaaagtaaa gaaaatttgg ttggcaaagc caactgcagc cagaccctct
     9721 ttaccaataa ttctgctgat cattaatgta tcggctaccc ccagcattgt atttaaactc
     9781 atctctaata ttgccggtaa ggccaggata aatatctttt tagccctaat ttttgagttt
     9841 tctgttaatc ttaattccat gattttaact ccttacaaca attacaaaat ttactttttt
     9901 taatttgaac atagtaatga taacacaact taatataaag ataaagtact ttaattaaat
     9961 ttttaacaaa attaataaaa ggcgttaagt tgtttataaa aagggtttat gataaaatat
    10021 aaaaaaatat gatttaagca attaggggca aataaaaatg aaagaaattg aaattacagt
    10081 agaaaatact tatgaaggaa tgagattgga taaatacatc tctaaaatta aaaaggattt
    10141 tagtagaaat cagattcaaa gtttaataga taaaaatctt gtagaggtaa atgacaacct
    10201 agagaaaaaa agttaccaag taaaaaccgg tgatgaaatt gttatatata aagaagaacc
    10261 gaaagtatta gacttaccta aaaaggaaat ggacctagat ataatttatg aagatgaaga
    10321 tattatttta attaataaac cggtcggttt aattgtgcat cccgttaaag gtaatagaac
    10381 agatacccta gtcaatgcat tattagcaca tacagaaaaa ctaggtaata ttaatgggac
    10441 cttaagacca ggtattgtcc acaggctaga taaaaacacg agtggagtta ttttagttgc
    10501 taaaaatcag cagagtctta ataatttaca aaaacagttt aaagaaagga agaccaaaaa
    10561 gatatatagg acaatattaa aggggaccct gccttataac agtgggacga ttgatgcacc
    10621 gattggcaga aaccctaaaa atagaaccaa gatggctgtg atcaaagaaa acagtaaaaa
    10681 ggctgtaact gaattcaagg taatgaaaag atataataat tatacttatg tagaagcgga
    10741 tttgaaaacc ggtcgtaccc accagattag agttcacttt gctcatctag gttttcacat
    10801 tataggggac caaaagtacg gttcaaaaga agaaaagttt gctttaaaac accaattatt
    10861 acatgcttat aaaataggtt tttatcaccc acaaagtaac gaatgggttg aatataaagc
    10921 agatttaccg gaacactttc aaaatatttt aaaaaaatta aaaaatttgt aaaaatctaa
    10981 ggagggcaaa aatgagttat tctaacaagt tatctgataa tgtttattgg gttggggtaa
    11041 atgatagaga aacagatttt tttgaaaatt tatggcccct accatatgga atttcctata
    11101 attcctatct aattgttgat gaaaaagttg tattagtcga taccgttaaa attgattatc
    11161 aggaaagata ccttgaaaag ttaatggatc taacagacgg cagaccaatt gattatctag
    11221 ttataaacca catggaacct gaccactccg gttcgatcaa ggaattaatg aggttattcc
    11281 ctgatatgaa gattattggt aataataaga ccactggatt tttaaaaggt ttttatggaa
    11341 tcaccgaaaa tattaaagag gtagataatg aagaaacttt agacatagga gaaaaaacat
    11401 taaagttttt tattacacct atggtacatt ggcccgaaac aatgatgacc tatctagaag
    11461 aagaaaatat attattctcc ggtgatgtat ttggtgcctt tggtgctata aaccaatcaa
    11521 tctttgacga tgaaatagag actgatttct ttgaagatga aattataagg tattattcta
    11581 atatagtagg gaaatattct ccgattgcag ccaaacatat tggcagctta ggagatttag
    11641 aaattgatac cgtagcttct acacacggtc cggtttggag agaaaaccca gattatatta
    11701 tcaataaata ctatgatatg gctaaattta aagctgataa gggtgcagta gttatctatg
    11761 gaacaatgta tggtaatact aaaaagattg cagaagaagc tgcggctggg ttaagtgcag
    11821 ccggagtaaa agatgtaaaa ctttatgatg cttcccggac ccataaatcc tatttaataa
    11881 gtgatatttg gaagtataaa ggggttatat ttgccagctg cagttataat aatgagctat
    11941 tcccgcctgt caattcttta gttgaagcac ttgaaaatag gggaattcaa aatcgttact
    12001 ttggtttcat agggacctac agctggagcg ggggttctct tgaaagaatg aatcaatttg
    12061 ctgaggattg taatattgag gtagttgaac ctaaaataaa taatcaatat gctcctacag
    12121 ccgataattt agacaaagca tttaagttag gtaaaaatgt aggagagaag atataataag
    12181 ggggtatatt taaaagaatg gatgttaaag agactataga aaaaagaaga tcttacagat
    12241 cattagattc cgttgaaatc actgatgaat taatt
//
LOCUS       DATGCJ010000092         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_234815, whole genome shotgun sequence.
ACCESSION   DATGCJ010000092 DATGCJ010000000
VERSION     DATGCJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234815"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VKN64_04800"
     CDS             <1..376
                     /locus_tag="VKN64_04800"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMA59593.1"
                     /translation="ANYVKIADIKEVLKQKYELTPAQVMGNGPAKYYKVKGYKGKLGF
                     ISPFGHNICSDCNRLRLTADGKVRSCLLKEEETSLYNEKKLVGRNKIKEIFLKSIQKK
                     SPHYGYEEVFANNNKNMNSIGG"
     gene            381..866
                     /gene="moaC"
                     /locus_tag="VKN64_04805"
     CDS             381..866
                     /gene="moaC"
                     /locus_tag="VKN64_04805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HMA59594.1"
                     /translation="MSEFSHIDQEGKAKMVDITEKEKTNRKALAKGKIKMKENTLSMI
                     KNENIQKGPVLETARIAAIMAVKNTARTIPMCHPIAIGGIDVNFVYKDNNSIEISVTV
                     KTYDKTGVEMEALNGVSTAALTIYDMCKAVDKEIVIGNIRLIEKTGGKSGHYQRKDGE
                     N"
     gene            868..1311
                     /locus_tag="VKN64_04810"
     CDS             868..1311
                     /locus_tag="VKN64_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HMA59595.1"
                     /translation="MDGKVKAVCISREKGTKKEDIKEAKLIENYGIKEDAHAGKWHRQ
                     VSLLGEESINKMKEMGLDYNLNYGDFAENITTEGIKLYELPIGTKLKVGENVVLEVTQ
                     IGKECHHGCEILKIVGKCIMPEEGIFTKVLKGGKIKAGDPIKIDK"
     gene            1431..1673
                     /locus_tag="VKN64_04815"
     CDS             1431..1673
                     /locus_tag="VKN64_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMA59596.1"
                     /translation="MKATGNLRRVDEMGRVVIPKKLRDSLNLQSEDSIEIFVEEDKIV
                     LQKYEPGCLFCDSTESTIEYMGKQICLDCLKEMDNS"
     gene            1736..1933
                     /locus_tag="VKN64_04820"
     CDS             1736..1933
                     /locus_tag="VKN64_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HMA59597.1"
                     /translation="MANCQNHQANMDYCNCTYEPCPRKGKCCECIRYHKKMGELPACF
                     FPDEAEKTYDRSIEHFVEIHS"
     gene            2297..>3441
                     /locus_tag="VKN64_04825"
     CDS             2297..>3441
                     /locus_tag="VKN64_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HMA59598.1"
                     /translation="MEKLYLLDGHSLAHRAFYALPLLTTESGEYTNSVFGFSRMLFKI
                     IDEEKPDHIAVAFDLKGPTFRHEEYEDYKAGRKKTPDELIPQIDLIKEVLKSLNIPII
                     EKEGYEADDVIGTIAKEAAEKDIDVKIVTGDRDALQLIDERINVIYTRKGITDIVEYN
                     IDKVKEKYELSPEQLIDMKGLMGDSSDNIPGVPGIGEKTAIKLLKEFDSMENVLENIE
                     KVSGKKRKENLRKYSEQAKMSKKLGKIVTDLSVDLEFEDLKLEEPNFNQVIQLFKRLE
                     FNSLLDRFEDEIEEKLKEISYSSIDKAEDVQEIVENIKESKKFAFTIDEDEDELLIKL
                     AGQIYKFKPFSEYKDNFKDIFEDIEIKKLSFNSKENILYLIKNDVNC"
BASE COUNT         1371 a          421 c          670 g          979 t
ORIGIN      
        1 agccaattat gtaaaaattg ctgatataaa agaagtatta aagcaaaaat atgaactgac
       61 accagctcaa gtaatgggta atggacctgc taaatactat aaagtaaagg ggtataaagg
      121 taaattaggt tttatctcac cgttcggtca taatatctgt tcggactgta atagattgag
      181 attaactgcc gatggtaagg tccgttcctg tttattaaaa gaagaggaaa cttccctcta
      241 taatgagaaa aaattagtag gtagaaacaa gattaaagaa atattcttga aatctattca
      301 gaaaaaaagt ccccattatg gatatgaaga ggtatttgca aataataata aaaatatgaa
      361 tagtattgga ggttaattaa atgagtgaat ttagccatat tgaccaagag ggtaaagcta
      421 aaatggttga tataactgaa aaagaaaaaa caaaccgtaa agctcttgcc aaaggaaaaa
      481 ttaaaatgaa agaaaacact ctctcaatga ttaaaaatga aaacatacaa aaaggccctg
      541 ttttagaaac ggcccggatt gctgcaatta tggctgtaaa aaacactgcc aggacaatac
      601 caatgtgtca cccgatagca atcggtggta ttgatgtaaa ctttgtatat aaagataata
      661 attctatcga aatatcagta actgtaaaga cttatgataa aacgggggtt gaaatggaag
      721 ccttaaatgg agtttctaca gctgccctaa ctatctatga tatgtgtaaa gcggttgata
      781 aagagatagt tatcggtaat attagattaa tagaaaagac cgggggtaag agcggccatt
      841 accaaagaaa agacggtgag aattaagatg gatggtaaag taaaagcagt ttgtatcagt
      901 cgggaaaaag gtaccaaaaa agaagacatc aaagaagcta agttaataga aaattatgga
      961 ataaaagaag atgcccatgc gggtaaatgg cataggcagg tcagtttact gggtgaggaa
     1021 agtatcaata agatgaagga aatgggattg gattataatt taaattatgg tgattttgct
     1081 gaaaatataa caacagaagg aattaaatta tatgaactac caattggtac taagttaaaa
     1141 gttggagaaa atgttgtatt agaggttaca cagatcggta aggaatgtca ccacggttgt
     1201 gaaatattaa aaatagtagg taaatgtata atgcctgagg aaggtatttt tactaaagta
     1261 ttaaaaggcg gaaagataaa agctggtgat ccgataaaaa tcgacaaata atactagaga
     1321 gaattatcaa taaaaagggc cttcatttat caaaatatat gaaaatatta ataaccattg
     1381 cacctatttc ctaaagatgt taatatatag aaaaaattgg agtgagaaaa atgaaggcaa
     1441 caggaaactt aagaagagtc gatgaaatgg gcagggtagt aatacccaaa aaattaagag
     1501 atagtttgaa tttacaaagt gaggattcga tcgaaatatt tgttgaagag gacaaaatag
     1561 tattacaaaa gtatgaaccg ggctgtcttt tttgtgatag tactgaaagt accattgaat
     1621 atatgggcaa acaaatatgt ttggactgtc ttaaagagat ggataattct tgacaagtta
     1681 tggttgagat tatacattta agatgagaaa aaatatataa aatagaggtg attatgtggc
     1741 aaactgtcag aaccatcaag ctaatatgga ctattgcaac tgtacatatg aaccctgtcc
     1801 ccgcaaagga aaatgttgtg aatgtataag ataccataag aaaatggggg aactacccgc
     1861 ttgtttcttc cccgatgaag cggaaaagac ctatgatagg tcaattgaac attttgtaga
     1921 aattcattct taaataacat tcccggtgaa caaccgggat tatctttttt cagaatttta
     1981 taaagattag aaattaggat atttacttta ctttttatat taaatggagt aaaatactca
     2041 tactaacagg aattttatag aggtaaatta aaaccgctat acttcaattt tataacagcg
     2101 gtttttttat agaatatata ttaagtgggt aatggaaagt acataataat ataattatat
     2161 aaattaaatt gaaataacta tcaccctaca atccgggata ttatatctaa atctctgatt
     2221 aaattatact cttctcatct tagcagggat tttctcttct ttcaggaata taatatctta
     2281 gaggagatga tatataatgg agaagttata tctactagat ggacacagtc ttgcccaccg
     2341 agccttctat gctttacctc tcttaaccac cgaatcaggt gaatacacaa attccgtatt
     2401 tggattttcc agaatgcttt ttaaaattat agatgaagaa aaaccggacc atatagctgt
     2461 tgcgtttgat ttaaaaggtc ctacttttcg ccatgaggag tatgaagact ataaagctgg
     2521 tcgtaaaaaa actcctgatg aactaatacc ccaaattgat cttattaagg aagtattaaa
     2581 atctttaaat attcctatta tagaaaaaga agggtatgaa gcagatgatg taattggtac
     2641 aattgccaag gaagctgccg aaaaggatat agatgttaaa atcgtaacgg gagacagaga
     2701 tgctctccag ttaatagatg aacgcatcaa tgtaatttac acccgaaaag ggataactga
     2761 tattgttgaa tataatattg ataaggtgaa agaaaagtat gaactgagcc ccgaacaatt
     2821 gatagacatg aagggtttaa tgggagatag ctctgataat attccgggtg ttcccggtat
     2881 cggagaaaag accgctatta aattattaaa agaatttgat agtatggaaa atgttttaga
     2941 aaatatagaa aaagtttccg gtaaaaagag aaaagaaaac ttaagaaaat attctgaaca
     3001 ggccaagatg agtaaaaagt taggtaaaat agtaaccgat ctttctgtag acttagaatt
     3061 cgaagattta aaattagagg aacccaactt taatcaagtt atacaactat ttaaaagact
     3121 cgaatttaat tctcttttag atcgttttga ggatgagata gaagaaaagt taaaggaaat
     3181 aagttatagt tcgatagata aagctgaaga tgtccaagaa attgttgaaa atatcaaaga
     3241 gagtaaaaaa tttgctttta ccattgatga agatgaagac gagttattga tcaaattagc
     3301 gggacagatt tataagttta aaccctttag tgaatataaa gataacttca aagatatttt
     3361 cgaagatata gagataaaga agttgtcttt taacagtaaa gaaaatatat tatatcttat
     3421 taaaaatgat gtaaactgta a
//
LOCUS       DATGCJ010000093         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_234911, whole genome shotgun sequence.
ACCESSION   DATGCJ010000093 DATGCJ010000000
VERSION     DATGCJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234911"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VKN64_04830"
     CDS             complement(<1..437)
                     /locus_tag="VKN64_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59599.1"
                     /translation="MSKKSLKVYTLLIIILTISAVISVYLPQGDIIDIQQQLPASKGI
                     VALVNALIMIVLYGGLGYVGLRLSLSLGFPELWDQNVNIKQRFLLPAIYGIIAGILLI
                     LFDYIFSFYNGIGKLVHPPFPTSFFASLSAGIGEEIIFRLFFIP"
     gene            complement(464..928)
                     /locus_tag="VKN64_04835"
     CDS             complement(464..928)
                     /locus_tag="VKN64_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59600.1"
                     /translation="MKKLLLFLFILIIVSAALNGLWEYGQCGIFYTINGEASFEHYNL
                     LIGRIILDIGVTLLLYIFVSIVNFEWKWFANWDLKDTFIILLFALFVSFYVEVNALYL
                     GRWGYSNIMPLLIGTDVGLTPILQWLITMPVSLLIVRLFMRGRVQTSSRYRF"
     gene            complement(1047..1370)
                     /locus_tag="VKN64_04840"
     CDS             complement(1047..1370)
                     /locus_tag="VKN64_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59601.1"
                     /translation="MLTKRLKIAVGVGAILGIFCIIGVGYRQGYAGNGLYLFAMWYNR
                     IIMGLLIGFAGNWMKDRYIIRGLILGTLVTTAFFLSTAFRDWPGFLAGIAYGVIIDYF
                     ATKYS"
     gene            complement(1448..1573)
                     /locus_tag="VKN64_04845"
     CDS             complement(1448..1573)
                     /locus_tag="VKN64_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59602.1"
                     /translation="MEFVGFLALPAFVFALSALSQAGKLKDRVTELEKELQKLNK"
     gene            complement(1590..2804)
                     /locus_tag="VKN64_04850"
     CDS             complement(1590..2804)
                     /locus_tag="VKN64_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59603.1"
                     /translation="MKDSLKVAKWEIKTIIKNKTFIIMTFFVPLIILIIGVSVGYFSA
                     RTEETDKLVVGINNSSKVITNNELEALETGNVDFIFYDNLDEGEIAQEIENNNLSSFL
                     VIPQGVLETNTVNYFFSDIQGIETDRIKELINPIIVEARIERSGYRTEVINNLVKEID
                     INSKSLSLEGNANSNVLEMFLPFGLAMLMVLASIFSGASLMQSIIKEKSNRIVEIILS
                     SINSRDLMMGKVLGYGVIGLIQITIWAVSALTVLKFVFEFSLTSLFTVKIMYMFIFFV
                     LGFILISAINAIVGSGMKEAQSGSQSTGFMVMIPMIPLYFSSLIINNPEGLLSYIFTY
                     IPVTSATTMLIRMGFSNPPLIEILGVMVLLAVANYLFIKLAAKIFRVGMLMYGKNANL
                     NELFKWARSSDY"
     gene            complement(2794..3705)
                     /locus_tag="VKN64_04855"
     CDS             complement(2794..3705)
                     /locus_tag="VKN64_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA59604.1"
                     /translation="MSEYVMNINNIEKSFQNTKAVDKVSFEVKKGEVLGLLGPNGAGK
                     TTIIRMIMDIFQPDTGEIKLNRDLIPDQKKIGYLPEERGIYDSTKVLDTILYFADLKG
                     MNKKEAKSEALRWLELFDLEEYKNNKIEDLSRGMQQKVQFIISVIHKPKLLILDEVFS
                     GLDPVNQELFKEIISNLTKDGTTILLSSHRMNLVEEVCSRIFMINKGKRVLYGNIDEI
                     KDGYNEKKVILKSKSDVGFIKNIVNVSDIKIENNEVTFNIKNDVNIKEFLSFLSKKID
                     VDEISIVKPPLHEIFVNAAKGGNNIEG"
     gene            3967..4314
                     /locus_tag="VKN64_04860"
     CDS             3967..4314
                     /locus_tag="VKN64_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin-associated ferritin-like protein"
                     /protein_id="HMA59605.1"
                     /translation="MSEYHELEEKMDEKTLDTARILNSLKEEVEAVNWYNQRAAATSD
                     PDVKAILEHNRDEEIEHAAMALEWLRRNVPAWEEELGTYLFTEAPITEIEEEGEGEEE
                     SEDSGGLDIGNMK"
     gene            4337..5128
                     /locus_tag="VKN64_04865"
     CDS             4337..5128
                     /locus_tag="VKN64_04865"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HMA59606.1"
                     /translation="MDLLKRDLAPLTEKAWEEIDDRAAEVFKTQLTARKSVHVEGPKG
                     IDFNAISEGKLGIIKGNKDEVRSSKYKVTPLLEARYTFELNRWEMDNLERGAKDVDLG
                     PLEEAVEKMAEFEDNAVYNGFEAGNIKGLMDHAKKSLSFGKDDSSIMDAITEGMIMLQ
                     DVYADKPYSLIVGKEAYSRLQKQVKGYPLIRRVEELIGSEVVFSEEIEGALLIPYDHD
                     DLELTIGQDFSIGYEEHDSKNVQLFISESFMFRVIDPEIIVKYTV"
     gene            complement(5201..>5436)
                     /locus_tag="VKN64_04870"
     CDS             complement(5201..>5436)
                     /locus_tag="VKN64_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59607.1"
                     /translation="EKNGYLEPFVGIYKKNIKVEVLDLIREEKLNFDNLFEKIKINRI
                     AEDKIEEIADTDRIFFNINKKEDLIKYKNFKAK"
BASE COUNT         1931 a          949 c          767 g         1789 t
ORIGIN      
        1 ggaatgaaaa acaacctgaa tataatctct tccccgatcc cggctgataa agaagcaaag
       61 aaagaagttg gaaagggtgg atgcacaagt ttcccaatcc cattataaaa gctaaatata
      121 taatcaaata atattaataa aattccagct attataccat aaatagctgg aagtagaaaa
      181 cgctgcttaa tatttacatt ttgatcccaa agttctggaa accccaagct taaagatagt
      241 cttaaaccta cgtaacctaa accgccgtat aggactatca taattagagc attaactaag
      301 gctactatcc ctttactggc cggtaattgt tgttgaatat caataatatc accttgaggt
      361 aaatagacag atatcacagc cgagatagtt aaaataataa tcaataaggt atataccttg
      421 agtgattttt tactcaatac gttcacccca tagtaatttt aagttaaaat ctatagcggc
      481 tagaagtttg cacccttcct ctcataaata atctaacaat taagagagaa actggcatag
      541 tgattaacca ttgaagaatg ggagttaaac cgacatctgt accaatcaat aagggcataa
      601 tattagaata gccccatcta cctaaatata aagcatttac ttccacataa aagcttacaa
      661 aaagagcgaa taataaaatt ataaaggtgt ctttaagatc ccagttcgca aaccatttcc
      721 attcaaaatt tacaattgaa acaaaaatat ataataataa agttactcct atatcaagaa
      781 tgattctacc aatcagtaga ttataatgct caaatgaagc ctcaccgttg atggtataaa
      841 agatgccaca ctggccatat tcccaaagtc catttaatgc tgcagataca ataattaata
      901 tgaataaaaa aagtagtaac tttttcaata tgaaatcctc cctagggtaa gttttatatt
      961 tctatattaa tataattata gaagagtccc ctatttcctt tatgaaatta tattacaatt
     1021 cattaatttt aaacccagtg aactgattaa gaatattttg tggcaaagta atctataatt
     1081 acaccatagg cgatcccggc caaaaagccc ggccagtctc taaaagcagt agataaaaag
     1141 aaagcagttg ttactaaagt acctaaaatt agacctctta ttatatacct gtctttcatc
     1201 cagttaccgg caaagccaat taataagccc ataataatcc tattatacca catggcaaag
     1261 agatataatc cattaccggc ataaccctga cggtaaccga cccctattat gcagaaaatt
     1321 cccaagatag ccccaacacc tacggctatt tttaatcttt ttgtaagcat aataatgcct
     1381 ccattaattt gatttatttt aacctctgct ttcttatttc tctaatttgg tatagtctcc
     1441 tttattatta tttatttaat ttttgtaact ctttttctaa ttccgtaact ctatctttta
     1501 acttaccggc ctgtgataaa gctgataaag caaatacaaa ggccggcaaa gctaaaaaac
     1561 ctacaaattc cattttttca ccttctttct taatagtcag aactccttgc ccatttaaaa
     1621 agttcattta aattggcatt tttcccatac attaacatac ccactctaaa gatcttagca
     1681 gcaagtttaa taaatagata atttgcaaca gctaataaaa ccataacacc caaaatctct
     1741 attaagggag ggttagagaa acccatcctt ataagcatag tcgtggccga agtaactgga
     1801 atatatgtaa aaatataact cagaagacct tctgggttat ttattatcaa gctagaaaaa
     1861 tacaagggga tcataggaat cattaccata aaacccgttg attgagaacc ggattgggct
     1921 tccttcatac cggaaccaac aatggcattg atagcagaaa ttaagataaa gcccaatacg
     1981 aagaaaataa acatatacat tatttttact gtaaataagg atgttaaaga aaattcgaat
     2041 acaaacttta atacagttaa ggctgaaacc gcccaaatcg taatctgtat taaaccaata
     2101 accccataac ctagtacttt tcccatcatt aaatcccgcg aattaataga agatagaata
     2161 atctcaacta ttctattgct tttttcttta attatactct gcatcaggct ggcacctgaa
     2221 aagatagatg ctaataccat gagcatggca agtccgaaag gcaaaaacat ctccaaaaca
     2281 ttagaattag cattaccttc taaacttaag gatttagaat taatatctat ttctttcacc
     2341 aagttattta ttacctctgt cctataaccg gacctttcaa tacgggcctc cacaataatt
     2401 ggatttatta gttctttaat gcggtcagtt tctattccct ggatatcact gaaaaaataa
     2461 ttgactgtat tcgtttctaa tacaccttgt ggtattacta aaaaactact taaattatta
     2521 ttttctattt cctgagctat ctctccctca tctaaattat cataaaaaat aaaatcaaca
     2581 ttgcctgttt ctaaagcttc taattcatta tttgttatta ccttagagct attattaatg
     2641 cctaccacta atttatctgt ttcttcagta cgggcagaaa aataaccaac actaacccca
     2701 ataatcaata ttattagggg aacaaaaaag gtcattataa taaaggtttt atttttaata
     2761 atagttttga tttcccattt agctactttg agactatcct tcaatgttat tacctccctt
     2821 cgctgcatta acgaatattt catgtagagg gggtttaact attgaaattt catcaacatc
     2881 aattttcttg gaaagaaatg ataaaaattc tttaatatta acatcatttt ttatattgaa
     2941 agtcacttca ttattttcta ttttaatatc agaaacattt actatatttt taataaaacc
     3001 aacatcactt ttggacttca aaattacttt cttttcatta tatccatctt taatttcatc
     3061 tatattacca tataaaactc tttttccttt attgatcata aaaatacggc tgcatacttc
     3121 ttctaccaga ttcatcctat gagaggaaag caatattgtc gtaccatctt tagttaaatt
     3181 actgattatt tctttaaata attcctgatt tacaggatct aaaccagaaa aaacttcatc
     3241 taagatcaat aatttaggtt tatgtataac tgaaattata aattgaactt tctgctgcat
     3301 tcctcttgat aagtcttcta ttttattatt tttatactct tctaaatcaa acaattctaa
     3361 ccatcgcaag gcttcacttt tagcttcttt tttattcatg ccttttagat ctgcaaaata
     3421 aagaatagta tctaatactt tggtcgaatc ataaatacct cgctcttccg gtaaatagcc
     3481 gatctttttt tgatcaggaa ttagatctct gtttaatttt atttctccag tatcaggctg
     3541 aaatatatcc attatcattc ttataatagt agttttaccg gctccattcg gtcctaataa
     3601 ccccaatact tctccttttt ttacctcaaa ggaaacctta tctactgctt ttgtattttg
     3661 aaatgatttc tctatattat ttatattcat cacatattct gacatgctta tacctccaaa
     3721 gaaatatttt tcactatcaa ttttattcta attgaatatt atttgataat caagcatttt
     3781 aattaatatt aattaattct taatattttt aagataatta taataaatct ctattttgta
     3841 aaatgcttac ttttttaaag taaatatatc taatataatt cttgttgaat gaaggatatt
     3901 cgtcctttta attgaataat atacattaga aaagtggaac aaaatataaa ggaggttata
     3961 aatgtaatgt ctgaatatca tgagttagaa gaaaagatgg acgaaaaaac ccttgataca
     4021 gctagaattt taaatagttt gaaagaggaa gttgaagcag tcaactggta taaccagcgt
     4081 gcagctgcta ctagtgatcc cgatgttaaa gcaatattag aacacaaccg cgatgaggag
     4141 attgaacacg cggccatggc tttagaatgg ttgagaagaa acgttcccgc ctgggaagaa
     4201 gaattaggta cttatctctt caccgaagca ccaattactg aaattgaaga agaaggtgaa
     4261 ggtgaagaag aatcggaaga ttcaggtgga ttagacattg gtaacatgaa ataaatatcc
     4321 ttaaggaggt atgaaaatgg atttactaaa aagagatctt gctccattaa cagaaaaagc
     4381 ctgggaagaa attgatgata gagcagccga agtatttaaa actcaactaa ctgctcgtaa
     4441 atcagttcat gtagaaggtc ccaaaggtat agattttaat gcaatttctg aaggtaaatt
     4501 gggaataatc aaaggcaata aagatgaagt aagaagcagc aaatataagg taactcccct
     4561 tttagaagcc cgttatacat tcgagttaaa tagatgggaa atggataatc tagaaagagg
     4621 ggcaaaagat gtagacttag gaccactcga agaagccgta gaaaaaatgg cagaatttga
     4681 agataatgct gtatataatg gatttgaagc aggtaacatc aaaggcctaa tggaccatgc
     4741 caaaaaatca ttatccttcg gcaaagatga ttcttcaata atggacgcca ttactgaagg
     4801 catgataatg ctgcaggatg tttatgcaga taagccttac agcttaatcg ttggtaaaga
     4861 agcttattca aggttgcaaa aacaggttaa gggttatccc ctcatccgca gagttgaaga
     4921 attaattggc tcagaagtag tcttcagtga agaaattgag ggagcgcttt taatccctta
     4981 tgaccatgat gatttagaat taacaatagg tcaggatttc tcaattggat atgaagaaca
     5041 tgatagtaaa aatgtacaac tattcatctc cgaatccttc atgttcagag taatagatcc
     5101 tgagattatt gttaaatata cagtttaacc tcaaataata aattgattaa taatagatga
     5161 agtaaaggac agcacgttta gtgctgccct ttttaatgtc ttattttgct ttgaagtttt
     5221 tatatttaat caaatcttct tttttattga tgttaaaaaa tattctatcc gtatctgcta
     5281 tctcttctat tttgtcttct gctatcctat taatctttat cttttcaaac agattatcaa
     5341 aatttaattt ttcttctctt attaagtcca atacttcaac ttttatattt tttttataaa
     5401 tacccacaaa gggttctaaa tatccgttct tttccg
//
LOCUS       DATGCJ010000094         7039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_102862, whole genome shotgun sequence.
ACCESSION   DATGCJ010000094 DATGCJ010000000
VERSION     DATGCJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102862"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VKN64_04875"
     CDS             complement(<1..1059)
                     /locus_tag="VKN64_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA59608.1"
                     /translation="MLSELNIKNFALIDELNIELKPGFSVLTGETGAGKSIILDALDI
                     LMGARAYKEVIRTNESSAYIEALFYPDQKQELDEILSENGISPEDEMLLISREINEDG
                     RNKGRINGQLVTVSLIQEIAPYLVDIHGQHEQQSLLNEDTHLMILDQYIKNDIKDLKR
                     EVKDYFSEIQNIKNKLSNLDIDEQSRARKIDLYQFQIDEITKANLETGEDEELFKKYK
                     KLSNMEDIYALCGEIDSLINAEDYSQSGLMDKVGHYMKNLEEYKEYDDSLADFHQSLQ
                     NIYYELEELSFRLDDYISSAEFDKNELNKVEERLDLINRLKRKYGDSIEEILEYKDDL
                     KEKLSELKNQEKIIDELEN"
     gene            complement(1076..1528)
                     /gene="argR"
                     /locus_tag="VKN64_04880"
     CDS             complement(1076..1528)
                     /gene="argR"
                     /locus_tag="VKN64_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HMA59609.1"
                     /translation="MKNKRQLKILNLIKNEVISTQEELVERLEEEGIKVTQATISRDI
                     KKLGLIKIPDEDGGYKYALANQKSSAENKYWLKKMFNNLVADIDYSDNVILLKTVKGT
                     AGGLGEALDNANWRHIIGSVAGDDTLLLIVKPSNKTEEVFEDLKKLMV"
     gene            complement(1540..2406)
                     /locus_tag="VKN64_04885"
     CDS             complement(1540..2406)
                     /locus_tag="VKN64_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HMA59610.1"
                     /translation="MGNIKTIGLVLNTEKDNSLEVSKRLIELIKEKDLDYLLEKEGAK
                     ELDLNHKRASYDFLRKKADLIIVFGGDGTFLHTSLNFIGTGIPLMGINLGRIGFLTEI
                     ETNELGEALDHIIADNYRIENRNTLEVCLKRAGEDVQKKYAVNDVVINRAADGEMLKV
                     DMHINKEFVNSYRGDGIIVSTPTGSTAYSFSAGGPIINPQVKALLITPLCPHAVHVKP
                     MVISDDEIIEIDVEGQKDKIFLTTDGRDSIKIKQDDIVRIKSSQQEISLIKFPDRTFY
                     TILRNKMRVGLV"
     gene            complement(2415..3230)
                     /locus_tag="VKN64_04890"
     CDS             complement(2415..3230)
                     /locus_tag="VKN64_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HMA59611.1"
                     /translation="MASKERIDVLLTNRDFFPSRNKAQRAIMAGEVYVDNKLVDKPGT
                     RVLADAEIEIKGDSLPYVSRGGLKLEKALKIFDVNPNGKEAIDIGASTGGFTDCLLQK
                     GAKKVYAVDVGYGQLAWKLRQDERVEVIERTNFRYIEPEKLPVKVPLIVTDVSFISLR
                     LIIPKALEFLTAEGDIIALIKPQFEAGKDKVGKKGIVNDKKIHQEVITKLSQFFDEHD
                     LILKDLDFSPITGASSKNIEYLLHLQNTQGYENDLFKWGKKIEEVIDRAHREL"
     gene            complement(3209..5122)
                     /gene="dxs"
                     /locus_tag="VKN64_04895"
     CDS             complement(3209..5122)
                     /gene="dxs"
                     /locus_tag="VKN64_04895"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HMA59612.1"
                     /translation="MSELLDRVESPEDLKDFSHKELNKLSKEIRNFLLETISETGGHL
                     ASNLGVVELTVALHHHLKSPIDKIVWDVGHQSYTHKILTGRKDKMHTIRQKGGLSGYL
                     KRSESEHDIIEAGHTSTSISSALGLALARNKLNSTERIYSVIGDGALTAGMALEALNN
                     AGHLGVDMNVILNDNNMSISPNVGALSNYLTKVRTDPVLSKLKEDIEFIIERIPKIGG
                     GVSKSVDRVKKGLKYVFITGILFEELGFTYLGPIDGHNIEDLVRNFKKADDIKGPVLI
                     HVNTTKGKGYNLAEEKPSEYHGVSPFDISNGEAKSCKTYKTYSQVFGHTAVKIREKLG
                     NQVVGITAAMASGTGLDIFEKEYPDEFYDVGIAEQHAVTLSAGMARGGLRPLVSIYST
                     FMQRAFDQVVEDVALQNLPISLFLDRAGLVGDDGETHHGAFDLTYMRMIPNMVVMAPR
                     DENMLQHMIWTSVKHEGPTSVRYPRGEGYGVCLDEKLKQLEIGKAEIMTDPGDILILA
                     VGSMVYPSLEAVKDLKKMGINAEVIDARFIKPLDENLLRKKFVNYNKVITVEENVLNG
                     GFGSAVLEFIENENIKTISVKRMGLPDEFVTHGTQAELRAMYNLNSAGIAQTTADFFK
                     GNLEGEVWHPKNA"
     gene            complement(5242..6132)
                     /locus_tag="VKN64_04900"
     CDS             complement(5242..6132)
                     /locus_tag="VKN64_04900"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HMA59613.1"
                     /translation="MKKINEIIKTEKAKIDKKIAAKIDNYRAKSNSSLKNIIDSLEYS
                     LKAGGKRIRPILTVKVAEMLKGDLETAYDLGIALEFIHTYSLIHDDLPAMDDDDYRRG
                     QLSNHKVFGEGMAILAGDALLTLAFEVLSDLNLPAEDKVKIINLTAQMAGFRGMVGGQ
                     SLDLEFEDIRVDFDNLNNIHLLKTGALFKAAILGGAYCAQTTEKEMDALESYATKLGL
                     LFQITDDILDKTGDSEVLGKEVGSDEKSNKSTYVSLLGLKEAKSKAKETAESAKKELE
                     PFENSQFLIKLIDFILYRES"
     gene            complement(6202..>7039)
                     /locus_tag="VKN64_04905"
     CDS             complement(6202..>7039)
                     /locus_tag="VKN64_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA59614.1"
                     /translation="LGEKDASGRRKPIPIDGSEFEVEADTVIAAIGQSTDAPENVPTN
                     RWGDVDAKDENYHVEDNIFSAGDCVTGPATVVEAIGAGRQTADSIEAFLNGEEYEKDY
                     IINVSRGHWQDLTQDELVLVKEPVERERATLEYISEDKRVSTFAETNKTLTEEEVKKE
                     AERCIECSCSYKNDCQLKDYSEEYKAHPEAMVGEKVRFEYDMRHPEIIRDPGKCIKCE
                     ACIKVCKEVVNEYLLEFKERGYYTRVGTEFGKPLPLDCTDCGECIDICPVGALDWKNK
                     KR"
BASE COUNT         1923 a         1591 c         1087 g         2438 t
ORIGIN      
        1 gttttctagt tcgtcgataa tcttttcttg attttttagc tccgataact tttcttttaa
       61 gtcatcttta tattctaaaa tctcctcgat tgaatctcca tatttgcgct ttaacctgtt
      121 aatcaagtcc agtctttcct ccaccttgtt tagttcattt ttatcaaatt ccgcactgga
      181 tatataatca tcgagtctaa aacttaattc ttctaactca taatagatat tttgtaggct
      241 ttgatggaag tcagccaggc tatcatcata ctccttgtat tcttctaaat tcttcatgta
      301 atgcccaacc ttatccatca gaccggactg actatagtcc tctgcattta taagactatc
      361 aatttcaccg cagagagcat agatatcttc catattagag agcttcttat actttttaaa
      421 aagctcttca tcttctccgg tctctaaatt tgcttttgta atttcatcaa tctgaaactg
      481 ataaagatct atctttcttg ccctactctg ctcatctata tcgagattac tcagcttatt
      541 ttttatattt tgtatctcgc tgaagtaatc cttaacttct cgtttaagat ctttaatatc
      601 atttttaata tactggtcca gtatcattag atgagtatcc tcatttaaaa gactctgctg
      661 ttcgtgttgg ccgtggatat caactaaata tggtgcgatt tcttgaatta aggaaactgt
      721 taccagttga ccattaatcc ggcctttatt acgaccatct tcattaatct ctcttgagat
      781 taagagcatc tcatcttctg gactgatccc attttcactt aatatttcat ctaattcctg
      841 tttttgatcc gggtaaaaaa gagcttctat gtaagcagaa ctctcattgg tcctaattac
      901 ttctttatag gctcttgccc ccattaagat atcaagtgca tcaagtatta tcgattttcc
      961 tgcccctgtt tcaccggtta acacactgaa accgggcttt aattctatat taagttcatc
     1021 aattaaagcg aaatttttta tattcagttc agataacatt aatgtcctcc cattattaaa
     1081 ccattaattt ttttaaatct tcaaagactt cttccgtctt attggacggt ttaacaatta
     1141 aaagaagggt atcgtctccg gccacactac ctataatatg acgccagttt gcattgtcta
     1201 aggcctctcc caaaccaccg gctgtacctt ttacagtttt taaaaggata acattgtcac
     1261 tgtaatcgat atcggctacc agattattga acatcttttt taaccagtat ttgttttcag
     1321 cacttgactt ctgattggca agtgcgtact tatagccgcc gtcttcgtcc ggtattttaa
     1381 ttagaccaag ctttttgata tctcttgata tcgtagcctg tgtgacctta ataccttctt
     1441 cctccaatct ctcaactaat tcttcctgag tactaattac ctcattttta attagattta
     1501 agattttaag ttgtcttttg tttttcatcg gtttcccccc taaactaatc ctactctcat
     1561 cttgtttctt aatattgtat agaatgtcct atctggaaat ttaatcaaag aaatttcctg
     1621 ttgtgaagac ttgatccgta caatatcatc ttgtttgatc ttgatggaat ctcttccatc
     1681 ggttgttaaa aatatcttat ccttttgacc ctcaacatcg atttcgataa tctcatcatc
     1741 ggatattacc atcggtttca catggaccgc atgtgggcaa agtggagtaa tcaaaagggc
     1801 cttaacctgg ggattaatga tgggaccacc ggccgaaaaa gaataggccg tcgaacctgt
     1861 tggtgtggat acgataattc catctccccg gtaagaattt acaaactctt tatttatatg
     1921 catatcaacc ttcaacatct cgccatcggc tgccctatta atgacaacat cattgacggc
     1981 gtatttcttt tgaacatctt caccagctct ttttaaacat acttctaagg tattcctgtt
     2041 ttcaatcctg taattatcag caataatatg atcaagagct tcccctaatt cattggtctc
     2101 gatctcggtt agaaaaccaa ttctacccag attaattccc atcaagggaa taccggtccc
     2161 aataaaattt aaagaagtat gtaaaaaggt gccgtctccc ccaaaaacaa ttattaaatc
     2221 ggcttttttg cgcaggaagt cataactagc tcttttatgg tttaaatcta gttctttagc
     2281 tccttctttt tctaaaagat agtctaggtc tttttcttta attaactcaa ttaacctttt
     2341 tgatacctct aaagaattat ctttttctgt atttaaaaca aggccgatag ttttgatatt
     2401 acccatctcc tttattagag ttctctatga gctctatcta ttacctcttc gatcttttta
     2461 ccccatttaa acagatcatt ctcatatccc tgtgtatttt gcaggtgcag caagtactct
     2521 atatttttac tggaagcccc cgtaatggga gaaaaatcta aatcctttaa tattaagtca
     2581 tgttcatcaa aaaactgact taatttagtt attacttcct gatgtatctt tttgtcattt
     2641 acaatacctt ttttaccaac cttatccttg cctgcttcaa actgtggttt gatcagggcg
     2701 atgatatctc cctcggctgt taaaaactcc agggcttttg gtatgataag tctcagagaa
     2761 ataaaggaca catctgtaac gattaaaggc acttttacag gtaacttttc gggttctata
     2821 tagcggaagt tggttctttc aatcacttca acccgctcat cctgtcttaa cttccaggcc
     2881 agctgtccat agccaacatc gacagcatat actttttttg cgcccttttg caggaggcag
     2941 tctgtaaatc ctccggtcga agccccgata tcgatggctt ctttcccatt tggatttaca
     3001 tcaaatattt ttaaagcttt ctcaagtttt aacccacccc ggctgacata agggagtgag
     3061 tcacctttta tctctatttc ggcatccgct aaaaccctgg taccgggttt gtctaccagt
     3121 ttattatcaa catatacttc tcccgccata atggctcgct gggccttgtt gcgggaggga
     3181 aaaaaatctc tattggttaa aagaacatct atgcgttctt tggatgccat acttcaccct
     3241 ccagattgcc tttaaagaaa tctgcagtgg tctgggcaat tccggccgaa ttaagattat
     3301 acatggccct taactcagct tgggtcccgt gggttacaaa ttcatcaggt aaacccattc
     3361 tctttacgga gattgtctta atattttcat tttcaataaa ttcaagtact gcactaccga
     3421 agcccccgtt gaggacgttt tcctctacag ttattacctt attgtaattg acgaacttct
     3481 ttctcaacag gttttcatcg agaggtttta taaaccttgc atcaataacc tcggcattta
     3541 tccccatttt ctttaagtcc ttgacagcct ctaaagaggg ataaaccatc gagcctaccg
     3601 ctaagattaa aatatcgccg ggatccgtca taatttctgc ctttccgatc tctaactgct
     3661 ttagcttttc atcgagacag actccataac cctctccgcg tggatagcgt acagaggttg
     3721 gcccttcgtg cttaacagaa gtccagatca tatgctgtaa catgttttca tcacgaggtg
     3781 ccatcacaac catattggga atcatgcgca tataggttaa atcaaaggca ccatggtgag
     3841 tttccccatc atcaccgacc agaccggccc ggtctaaaaa gagagaaatc ggtaagttct
     3901 gtaaggcaac atcttctacc acttggtcaa aggctctctg cataaaggtc gaataaattg
     3961 aaactaaagg tcttaaaccc cctcttgcca ttccggctga aagggtcaca gcatgctgct
     4021 cggcaatacc aacatcataa aactcgtctg gatattcttt ttcaaaaata tctagtccag
     4081 ttcccgaagc cattgcagct gtaataccaa ccacttgatt tcctaacttc tctcttatct
     4141 taacagccgt atgtccaaat acctgactat aggtcttata tgtcttacag ctctttgctt
     4201 ccccattact gatgtcaaaa ggagaaactc cgtggtattc actcggcttt tcttcggcta
     4261 aattgtagcc tttaccttta gttgtattaa catgaattaa gaccggtcct ttgatatcat
     4321 cggccttctt aaagttgcgt accaggtctt caatattatg accatcaatc ggacctaaat
     4381 aggtaaaacc gagttcttcg aataagatac cggtaataaa tacgtacttt aaaccttttt
     4441 tgactctatc gacagactta gaaactcctc ccccaatctt gggaattctt tcgattataa
     4501 attcaatatc ttcttttagt ttacttaaga cagggtcggt tctcaccttt gtcagatagt
     4561 tagataaagc ccctacatta ggtgaaattg acatattatt atcatttaaa attacattca
     4621 tgtcaacacc aagatgtccc gcattattta aggcctctaa agccatacca gcagttaggg
     4681 ccccatcacc tatgacggaa taaatgcgtt ctgtactatt gagcttatta cgggccaaag
     4741 ctaagcctaa agcagatgaa attgaagttg aagtatggcc ggcttcaatt atatcatgct
     4801 cactctcgct tctttttaga taaccgctta aaccaccctt ctgacggatg gtgtgcatct
     4861 tatctttacg accggttaag attttatggg tataactctg atgacctaca tcccagacga
     4921 tcttatcaat gggactcttt aaatggtgat gtaaagccac cgttaattct actaccccta
     4981 aatttgaggc cagatggccg cctgtttcag atattgtttc taaaagaaaa tttcttattt
     5041 ctttcgataa tttatttaat tctttatgag aaaagtcctt taaatcctca ggactctcca
     5101 ctctatcaag cagttcactc atttctactt ccccctacag ttcttcacat tatattataa
     5161 ttattatata ttttacacaa attgttctgt ataaatttga aaagcataat aattataaca
     5221 taactaaagt atacctgcaa tttaactctc tctatataag ataaaatcga taagtttaat
     5281 caaaaattgg ctgttttcaa agggctctaa ttccttttta gctgactctg cggtctcttt
     5341 tgcctttgat tttgcctctt tcagcccaag aagagaaaca taagtacttt tattagattt
     5401 ttcatcacta cctacttctt tgcctaaaac ttcagaatca cctgttttat ccaatatatc
     5461 atcggttatc tggaataata agcctaattt agtggcataa ctttctaagg catccatctc
     5521 tttttcagtg gtctgggcac agtatgcacc acctagaata gcggccttaa ataaagcacc
     5581 tgtttttaaa aggtgaatat tatttaaatt atcaaaatca accctgatat cttcaaactc
     5641 taaatcaagg gattggcccc caaccatacc tctaaaaccg gccatctggg ctgttaagtt
     5701 aataatcttc actttatcct cagctggtaa gttgagatct gacagcacct caaaggccag
     5761 ggttaaaagc gcatcacccg ctaaaatagc catgccttct ccaaatactt tatggttact
     5821 taactgaccc cttctgtaat catcatcgtc catagccggt aggtcatcat ggatcaaaga
     5881 ataggtatga ataaattcta aggcaattcc cagatcataa gcagtttcta aatctccctt
     5941 taacatctct gccactttta cagttaagat cggtctgatt ctcttcccac ctgcttttag
     6001 ggaatactct aaggagtcta taatattttt taaagatgaa ttagatttag cacgataatt
     6061 atctatcttt gcagcaatct ttttatcgat cttagctttt tctgttttaa ttatttcatt
     6121 tattttcttc atcgatacca ccttttagct ttaaaaagag agaagggcaa attacaccct
     6181 tgctctcttt atctttattt tttatctttt cttgtttttc cagtccaggg caccaaccgg
     6241 acagatatca atacattctc cacaatcagt acaatccagt ggaagtggtt taccaaattc
     6301 ggtacccact cttgtatagt aacctctttc cttgaattct aagaggtatt cgttcactac
     6361 ttctttacag accttgatac aggcctcaca tttaatacat ttgccgggat ctctgatgat
     6421 ctcgggatgc ctcatatcat attcgaagcg gaccttttct cccaccatag cttcagggtg
     6481 agctttgtat tcttcggaat aatctttgag ctgacaatcg tttttataag aacaactgca
     6541 ttcaatacag cgttctgcct cttttttgac ctcttcttct gttagggttt tattggtttc
     6601 agcaaaagta cttacccttt tgtcttcaga aatatattct aaggtagccc gctctctttc
     6661 gaccggttct ttaaccagga ccagttcatc ttgtgttaag tcctgccagt gtccgcggga
     6721 cacattaata atataatctt tttcatattc ttctccattt aagaaggcct caatactatc
     6781 agcagtctgt cggccggctc caattgcttc tacgaccgta gctggaccgg ttacacagtc
     6841 tccggctgaa aagatattgt cttctacatg gtaattttca tcttttgcat ctacatctcc
     6901 ccaacgatta gttggtacat tttcaggtgc atctgtactc tgtccgatag cagctatcac
     6961 tgtatcagct tctacttcaa attcactacc atcaatcgga atcggtttgc ggcggccgga
     7021 agcatctttt tctcccagt
//
LOCUS       DATGCJ010000095         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_637989, whole genome shotgun sequence.
ACCESSION   DATGCJ010000095 DATGCJ010000000
VERSION     DATGCJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637989"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2624)
                     /locus_tag="VKN64_04910"
     CDS             complement(<1..2624)
                     /locus_tag="VKN64_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMA59615.1"
                     /translation="MFKNHIKILDKYIIKEIALPFLASVIIITIILLGNFLFQLTDLI
                     IVKDVPIPLVLELLLYNLPDIIVRTFPIAVLFSTMTGIGRLNRENEITAFRMGGISLY
                     RLIIPVLILGIVVSGLTFVFNEEVVPWSNHKAKNIIRNTILKESMPNTQEEVFFKGPK
                     GRLFYVKEYKEEEDLLQNIIIYNIKKNQDEENFPEVITANKGVINENVWVLQNGIIHE
                     YNSEGNIILESQFKQMEFQITEEMQEMYGNQKTTDEMSRKELAKNIKLFRKSGINVNS
                     LLVDYHLKLAQPLVAFIFVLIAVPLSLTNKKSRAVNITFTIIIIFGYYLLQSVCRSLG
                     RNELLPPLLAAWLPNIIFLLLGLLILFLQESFEKLLYKILPGLLVLFLIFTIIPINST
                     AAAEELTLNSKSVSYDPKQNMVKIEGEVNGKYENIYLRSELINVKVKGEGEKIFKQPE
                     NIELLPGDITGCDFEEPHYYFDARRIEISPNEYLKAYHVVFRELGGKLPLFYWPYLYI
                     SLEDDEQNFFPRVGYNSRRGWFLKTTYNYYLDRLDLPGSLYMDYYTKTGFAGGLKQYL
                     VHSSNHKSYLYYYTQENNINLPNLFNWEGEFYHQYKGENWQEEFSYFYQEYDEKEELE
                     TKLSFSNSIDDRNINIDTYYDSVDYYESNDNDIKSYGADISFRDRYFDSLDVDLGYDI
                     DYDYDPEDGLEEYKERTADIVNDWGNDWETGIEYEYEEAIEPEAEKQTRWGGKGYISK
                     AYSEYKYTLLWERYDPNFSAEDEVAFYKDPEFTAEYTPAGDFEYLFQFGHYFEADSQI
                     EGNRARLELIYDNNLNIGSFNSLNINQKFHSNLYKVEYLGEMYSPMQLVSDSEFALTT
                     ELGDHLTFNNKFNMVRYSG"
     gene            complement(2717..3460)
                     /locus_tag="VKN64_04915"
     CDS             complement(2717..3460)
                     /locus_tag="VKN64_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HMA59616.1"
                     /translation="MKKCFILILVFMFVFTSLTFSAALKVDNLDINFNIEPETIKNNS
                     FVPIEQFKELENFSMYSIEENRLLIIYNSNYYAFSLNNKEIRSNTGNFNICCKPIKLN
                     EHVLVPYELVEKIFAKEISETNDNKKLIDIELRLTQQKIDDKDVLDIEIILNNQAEEK
                     ITLEFSTSQKYNILIKDKNNDIIYNWEKGKMFTQAFTYNTIEKNSSIKFAEKIDVTDL
                     KAGTYNLIISMENNNYEIKNKEVEFIIDK"
     gene            complement(3480..>4404)
                     /locus_tag="VKN64_04920"
     CDS             complement(3480..>4404)
                     /locus_tag="VKN64_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMA59617.1"
                     /translation="NVEMIPIRVFKNKEAQTSDIAEAIEYSIDQNVDIINMSFGGSNT
                     YNTLSNTIGDSLKNASEEGIILIAAAGNTGGQILYPASAPETIAVGATDINNNIASYS
                     NTGPELDLVAPGGNGNGILSTSGYYQNGTEYTNEYVLMEGTSMATPYVSGVASLLLSQ
                     GVSPDNIKDRLTSTAVDLLPSGKDSTYGYGLVDAYGALLDKRLKKPYIFAATKENGSI
                     DIKSEFETVDRDNTYKLDEIVEDKVYIIGWRDVNDDKIINKGDYYGEYEKEINLLENS
                     LYNNIDIEMYYIPQNDNTNIEVMNMTEVKSE"
BASE COUNT         1352 a          777 c          600 g         1675 t
ORIGIN      
        1 cctgaatatc ttaccatatt aaatttattg ttaaaagtaa ggtgatctcc tagttctgtg
       61 gttaaagcaa attcgctatc agatacgagc tgcataggac tatacatttc tcccaaatac
      121 tctaccttat aaaggttaga atggaatttt tgattaatat ttaaggagtt aaaactacca
      181 atatttaaat tattgtcata aatcaactct aaacgtgctc tattaccttc tatttggctg
      241 tcagcctcaa agtaatggcc gaactggaat aagtactcaa aatcaccggc cggtgtatat
      301 tcggccgtaa attcggggtc tttataaaag gctacttcat cttcagccga aaagttagga
      361 tcatatctct cccacagtaa agtatacttg tattcactgt aagctttact gatataacct
      421 ttacctcccc atctggtttg tttttcagcc tctggttcaa tggcttcttc atactcatat
      481 tcaatcccgg tttcccagtc attaccccaa tcatttacta tgtcagccgt tctttctttg
      541 tattcttcta gaccatcttc ggggtcataa tcataatcta tatcataacc tagatctaca
      601 tctaaagaat caaagtatct atcacgaaaa gagatatcag caccatagga ttttatatca
      661 ttatcgttgc tttcatagta gtcaacactg tcataataag tatctatatt gatatttcta
      721 tcatcaatag aattactaaa agaaagtttg gtttctaatt cttctttttc atcatattct
      781 tgatagaagt aagaaaactc ttcctgccag ttttctcctt tatattgatg gtaaaattct
      841 ccttcccagt taaatagatt aggtaagttt atattattct cctgggtata gtaatataga
      901 taacttttat ggtttgaact atgaactaag tactgtttaa ggccgcctgc aaagccggtc
      961 ttcgtataat agtccatata gaggcttcca ggtagatcaa gtcggtctaa atagtaatta
     1021 taggttgtct ttaaaaacca gcctctacgg ctgttataac cgactctcgg gaagaaattt
     1081 tgttcatcat cttctaatga tatatataaa taaggccaat aaaaaagggg cagtttgccc
     1141 cctagttctc tgaatacgac atggtaagcc tttaaatatt cattaggaga aatttcaatc
     1201 cggcgggcat caaagtaata atgaggttct tcaaaatcac agcctgttat atctcccggt
     1261 agtagctcaa tgttttcagg ctgtttaaat attttttctc cttcaccttt aactttaaca
     1321 ttgatcagct cggatcttag ataaatgttt tcatattttc catttacttc tccctctatt
     1381 tttaccatgt tttgttttgg atcataactt acagatttac tgtttagagt caactcctca
     1441 gcggcagcag ttgaatttat cggtatgatt gtaaaaatca aaaataatac taataaaccg
     1501 ggtaaaattt tatacagtaa tttttcaaat gattcctgta agaataagat taataaacct
     1561 aacagtaaga atattatatt gggcagccag gctgctaata aaggaggtag tagttcattt
     1621 ctccccagtg atctgcagac ggactgcagt aagtaataac caaatattat gatgattgta
     1681 aaggtgatat taacagccct gcttttttta ttggttaaac ttagcgggac agcaattaat
     1741 acaaatataa aagcaaccag gggctgtgct aactttaagt ggtagtctac aagcagtgaa
     1801 ttaacattga tcccgctttt tctaaatagt ttaatatttt tggccagttc ttttcggctc
     1861 atttcatcgg tcgttttttg gttaccgtac atttcctgca tttcttccgt tatttgaaat
     1921 tccatctgtt tgaactggct ttctaatata atattaccct cactattata ctcgtgtatt
     1981 atcccatttt gtaaaaccca gacattttca ttgataacac ccttattggc agtaattact
     2041 tccggaaagt tttcttcatc ttgatttttc ttgatgttat atatgataat attttgtaat
     2101 aagtcctctt cttctttata ttcttttaca taaaaaagtc tgcctttcgg gcctttaaaa
     2161 aatacttctt cctgggtatt tggcatactt tcctttaaga ttgtatttct tattatattt
     2221 tttgctttat ggttagacca cggtactact tcttcattaa agacaaaagt aaggccgcta
     2281 acaacaatac ctaatatcaa tacaggtatt attagccgat aaaggctaat accgcccatc
     2341 cgaaaagcag taatctcatt ttccctgttt aatcttccga tcccggtcat tgttgaaaac
     2401 aatactgcaa tagggaaagt ccttactata atatcaggta gattatataa taataattct
     2461 aatactaagg gtataggtac atctttaact attatcaggt ctgtaagttg gaagagaaag
     2521 tttcccaata gtattattgt tattattatt acagaagcta aaaagggcag agcgatttct
     2581 tttattatgt atttatctaa tattttaata tgatttttga acattaaaac ccctcataat
     2641 tttctaaata actatattta ttaatcattc ttatttattt tatcataaat ttcttttgtt
     2701 agcatttatc tatactttat ttatcaatta taaattcaac ttctttattt tttatctcat
     2761 aattattatt ttccattgat atgattaaat tataagtacc agcttttaaa tcagtaacat
     2821 ctatcttttc tgcaaacttt attgaactat ttttttcaat tgtattataa gtgaaggcct
     2881 gggtaaacat cttgcctttt tcccagttat atataatatc attattttta tctttaatta
     2941 aaatattgta tttttgactg gtgctaaatt ctaaagtgat cttttcctca gcttgattat
     3001 ttaaaataat ttcaatatct aatacatctt tatcatcaat tttttgttgg gttagtctta
     3061 attcaatatc tattaatttc ttattatcat ttgtctcact aatttcttta gcgaagatct
     3121 tttctactaa ttcataagga accaatacat gttcgtttaa ttttatcggc ttacagcaga
     3181 tattgaaatt cccggtattt gacctgatct ctttgttgtt taaagaaaat gcataatagt
     3241 tggagttata aattattaac aatctgtttt cttctataga atacatcgaa aagttttcta
     3301 attctttaaa ctgttcgatt ggtacaaaac tattgttttt tattgtttca ggttcaatgt
     3361 taaaatttat atctaaatta tcaactttta aagcagcaga aaaagtaaga cttgtaaaaa
     3421 caaacataaa tactaatatt aatataaaac attttttcat aagccattca tctcctgtat
     3481 tactcacttt tgacttcagt catattcatt acttcgatat ttgtattatc attttgtgga
     3541 atataataca tctcaatgtc aatattgtta taaagggagt tttctaaaag atttatttct
     3601 ttttcatatt ctccgtaata atctccttta tttataattt tatcatcatt aacatctctc
     3661 caacctataa tatatacctt atcttccaca atctcatcta atttataagt attatctctg
     3721 tctacagttt caaattcact ttttatatca attgaaccgt tttctttggt agcagcaaat
     3781 atataaggtt ttttaagcct tttatctagt aaagccccat aggcatctac taaaccatac
     3841 ccatatgtac tatctttgcc tgaagggagg aggtccacag cagtagatgt aagtctatcc
     3901 ttaatattat caggacttac tccctggctt aataataatg atgctactcc actgacataa
     3961 ggtgtagcca ttgaagtccc ttccattagc acatattcat tagtgtattc tgttccgttc
     4021 tgataatatc cgctggtact taaaattcca ttaccatttc cacctggggc aaccaggtct
     4081 aattcaggac ctgtatttga ataagaggca atattattat ttatatcggt ggcaccaact
     4141 gctattgttt ctggagcaga agcgggataa agaatttgac cacctgtatt tcctgctgct
     4201 gcaataagaa tgattccttc ttcagaagca ttttttaatg aatctccaat agtattactt
     4261 aacgtattat aggtatttga accaccaaaa ctcatattga taatgtctac attctgatca
     4321 attgaatatt ctatagcttc tgctatatca ctggtttgag cctctttatt tttaaatact
     4381 ctaatcggaa tcatttcaac atta
//
LOCUS       DATGCJ010000096         8031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_369826, whole genome shotgun sequence.
ACCESSION   DATGCJ010000096 DATGCJ010000000
VERSION     DATGCJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369826"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1019
                     /locus_tag="VKN64_04925"
     CDS             3..1019
                     /locus_tag="VKN64_04925"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HMA59618.1"
                     /translation="MKGIKSNIKAEGSYTKMKEKSVGKDTTTGHWELAGLVSERPFPV
                     YPNGFPDEVIKSFEKKIGKEVLGNKPASGTVIIEELGEEHLNTGKPIVYTSADSVFQI
                     AAHVDIISVEELYEMCEIAREILQGEHGVARVIARPFVGELGSFTRTERRKDFSLAPP
                     RATVLDKLKARGISVMAVGKIEDIFSNKGITDSNHTVDNMDSVDAVIDYLKEDKKSFI
                     FANLVEFDMVYGHRRNAKGYAKALKDFDIRLPEIIDNMKEDDLLMITADHGCDPTFKG
                     TDHTREYVPLLVYGDKIKEDYQLPIRESFADIAATVAEVFNLEDFKNGQSFLSNIIKG
                     DNND"
     gene            1012..1824
                     /locus_tag="VKN64_04930"
     CDS             1012..1824
                     /locus_tag="VKN64_04930"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMA59619.1"
                     /translation="MIDKIEEAADYIEGEIKIEPEIALILGSGLGVLAEEIEDSTVIK
                     YEDIPNFPVSTVEGHAGQFVIGTLEDKKVMAMQGRFHYYEGYSMQEIALPIRVMYKLG
                     INNLVVTNAAGGINRNFDPGNFMVIQDHINLMGDNPLKGENFKEFGPRFPDMSEIYNE
                     DLRELAEKVAVENKIKTVKGIYVGLEGPSYETPAEIRYLRGTGADAVGMSTVPETITA
                     NHMGMNILGISCITNMAAGVLPKPLDHKEVIEIADKVKPKFSTLIKGILRKM"
     gene            1841..3148
                     /locus_tag="VKN64_04935"
     CDS             1841..3148
                     /locus_tag="VKN64_04935"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HMA59620.1"
                     /translation="MRVYDLILKKREGHELTKEEIDYLIKGFTNGNIPDYQMSAFAMA
                     VYFNGMTDRETADLTMAMVESGDQIDLAKIEGTKVDKHSTGGVGDTTSLVLVPLVSAA
                     GIPVAKMSGRGLGHTGGTIDKLESIPGFKTDLSMDKFINNVNNIKAAIAGQTGNLTPA
                     DKQLYALRDVTATIESIPMIASSIMSKKIAGGADGIVLDVKVGSGAFMKTLSNAKKLA
                     EIMVNIGQHLGRNTIAVLTDMEQPLGYAVGNNLEVKEAINTLKGEGPSDLRELCLTLG
                     ANMLKLAGKVDGFKEGYEILVEKLTKGEAFNQFKKIVENQGGDLSYIENPAKFKKADH
                     VFEVKAKSTGYLNQLKALDIGLAAMLVGAGREKKGDEILNEVGVILHKKYGEHVNKDE
                     IIASLHYNKDDDNLIEAKEKLKNSFVIKDEVPKQRDLIFEIVD"
     gene            3179..4498
                     /locus_tag="VKN64_04940"
     CDS             3179..4498
                     /locus_tag="VKN64_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HMA59621.1"
                     /translation="MSKFDGERLPVDIFQIDQERMRQGWYSDVYFRNVSRILTKLSEE
                     GYRFKDEDIGNLEVEMQIFPRRKPFCILGGIDEALSILKKSTGYEKEDGEFVQTFDEL
                     EVMACHDGEKGYYKGNPARVDPVMKIRGRYRDFAHLETPIIGSLTEASRIATNVYQVM
                     EAARGKDILFFPARFAHYKLQALHGYAYSLGVNAYNEDYGQNMTRYVSTDEQGAWWGG
                     KGGGTISHSYIATFLGNTTEAMLQFSRLMPKDVNRIALVDFDNDCVNTTLKVLKAMFD
                     KYIELKEAGKEEEASKYKLFGVRPDNSSSLRDKSIEPLGDKKLDNGVNARLVFKLRKE
                     IDRAYKDWVLKENRVEEAKQFCEDVKIIATGGFKPKKINEFEESQVPVDIYGVGSWLL
                     SNSSVEGTKNDFTADVVRVKKDGEWIDMAKVGRKACNNECMELVTEY"
     gene            4522..7128
                     /locus_tag="VKN64_04945"
     CDS             4522..7128
                     /locus_tag="VKN64_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMA59622.1"
                     /translation="MDLIISHENTDIDGLAGMIAASKLYTEAKVVVSENMTTLAQRFL
                     ALYKDEFEIFRYDDIDYEGIDRVIVIDTHEYDRLGKLTELINWDQVEVIVYDHHPHQK
                     LDWIDIDLSENVGSATSIIVNRLKRENIKLNRSEATLFALGISADTGNFMHLNTESED
                     LIAFAYLLDMGANKKAINEYLLETLDKNQKRVFELLIKHRKDIKIKDKDITLFTLKYP
                     RYVTGLNNVVEKIKLLYQLSVVFVLVEMEGKVIIIGRSSDENVNLEEIFSELGGGGHS
                     GAGSVSIKTDLEIARNKLETAIRDKIKVAVKVKEVMHEPTKVHESTKLIDVKKKMKKH
                     NITGVLVTNDEDKVKGIFTTRDLRRLNDEDELYRVPVKGYMTKKLFFIQPDDTIHKAQ
                     ERMVKKDIGRLPVKDDKGAVVGIITRRDILNQYYEEETENQNQNRYVSSLVSITPEKL
                     KIPDKLDQLSDEIRKLFKNIGKIAQDKDSRIFLVGGMVRDLLLEQENKDLDFVVDGNI
                     EGLIREIAAKYDVKYKYNENFQTGSMVIDNQYDLDFAVSRNELYTHPGALPKVQKADI
                     FDDLFRRDFTINALAIALHPAEYGYLYDFFKAREDVENRILKVLHRFSFLDDPTRIIR
                     GIRLVLQLDLDIEEETKALMQEAIRLSRFSDVTFPRIYKELRFLFAANPGLKFIELLR
                     EVPFLRLLYDDYKIPVNIEEKWQRADRYLAYFENNNYNVKKWPVYFSLILKDIPRSII
                     ERWSLNLKAKNIILFKPEEYEIDKITDSAGRVELYNNLKQFTNEELVLLLAEHDNEQL
                     QEKIFYYLENISDINIAIDGNDLIELGYQEGPIISKALRLIKRQVLKGNLQSRDKQLK
                     YAKKLKKKLERK"
     gene            7135..7776
                     /locus_tag="VKN64_04950"
     CDS             7135..7776
                     /locus_tag="VKN64_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMA59623.1"
                     /translation="MSNIMELILVIPVLLLSLSIHEYSHGYVSYLLGDPTPKANGRLT
                     LNPIAHLDLVGSLVLLITRRIGWAKPVPIDPRYYKNPRKGLMLVGIAGPGSNLILATI
                     FALIFRTVMAFASINSVGAMGTQVSNLQYVVLRFLILAVIVNLSLGFFNLLPIPPLDG
                     SNILRGLIPRDLDKYLNKLQGPIGMILLLVLAYTGILWGMISPFVNFFLGILI"
     gene            7798..>8031
                     /locus_tag="VKN64_04955"
     CDS             7798..>8031
                     /locus_tag="VKN64_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMA59624.1"
                     /translation="MDYEVQLDAFQGPLELLYQLVKKNKIEISQISLAKITGEYLDYI
                     DQHIMDLEYTSEFLVIAAELIEIKAKYLLPKTDE"
BASE COUNT         2668 a         1303 c         1837 g         2223 t
ORIGIN      
        1 ccattaaagg gatcaaaagt aatattaaag cagaaggtag ttatactaaa atgaaagaaa
       61 aatcggtcgg gaaagataca actaccggcc actgggaatt agccggtttg gtctctgaac
      121 ggccttttcc ggtttatccg aatggttttc cggacgaagt aataaaatct tttgaaaaaa
      181 agataggaaa agaagtatta ggtaataaac cggcttctgg aactgtaatt atagaggaat
      241 taggagaaga acatttaaat actggtaaac cgattgtcta tacctcagct gatagtgttt
      301 tccaaattgc ggcccatgta gatataattt ctgttgaaga actctatgag atgtgtgaaa
      361 tagcccgtga gatactgcag ggtgaacacg gagtagcgag ggtaattgcc cgaccctttg
      421 tgggagaact gggcagcttt accagaacag aacgcagaaa agacttttct ttggctccac
      481 cgcgtgcaac tgtgcttgat aagttaaaag ctcgtggaat ttctgtgatg gcggtgggca
      541 agatagaaga tattttttcc aataaaggga ttacagattc gaaccacacc gtagataata
      601 tggactcggt tgatgccgta attgactatt taaaagaaga taagaagtcc tttatctttg
      661 caaacctggt tgagtttgat atggtttatg gacacaggcg taatgcgaag ggttatgcta
      721 aggccttaaa agattttgat atcagactac cagagatcat agataatatg aaagaagatg
      781 atctattgat gataacagcc gaccacggct gtgacccaac ctttaaaggg actgaccata
      841 ccagagaata tgttccttta ttggtctatg gagataaaat aaaagaggat tatcagttac
      901 ctataagaga atcttttgct gatattgcag ctactgtagc tgaagtattt aatttagaag
      961 atttcaagaa cggccaaagt tttttaagta atataataaa gggggataat aatgattgat
     1021 aagattgaag aagctgctga ctatatcgaa ggtgaaatta agatagaacc agagattgct
     1081 ttgattttag gttcgggctt aggtgtatta gcagaagaaa ttgaagacag tacagttatt
     1141 aaatatgagg atattccgaa ctttccggtt tcgaccgtag aaggccatgc cggccagttt
     1201 gtaattggaa ctttagagga taagaaggta atggcaatgc agggtcgttt ccattactat
     1261 gaaggttaca gcatgcagga gattgcttta ccgataagag ttatgtataa attaggtatt
     1321 aacaatctcg tcgtaaccaa tgcggccggt ggtatcaata gaaactttga ccccggtaac
     1381 tttatggtca ttcaggatca catcaattta atgggggata atccccttaa gggagagaac
     1441 tttaaagaat tcggaccgcg ttttcctgat atgtccgaaa tatataatga ggatttaaga
     1501 gaattagctg aaaaagtggc agttgaaaac aagataaaaa cggttaaagg tatttatgtc
     1561 ggactggaag gtcccagcta tgaaacaccg gctgaaatac gctatttaag aggaacggga
     1621 gccgatgcag ttgggatgtc aactgtaccg gagaccataa ctgcgaacca tatgggaatg
     1681 aatatcttag gtatctcttg tataacaaat atggcggctg gtgtattgcc gaaaccgctt
     1741 gaccataaag aagttattga gattgcggat aaggttaaac ctaagttttc aactttaatt
     1801 aaaggtattt taagaaaaat gtaaaccggg aggttttaat gtgagggtat atgacttgat
     1861 tttaaagaaa agagaaggtc atgaactaac aaaagaagaa atagactatt taattaaggg
     1921 gtttacaaac ggtaatattc ccgattatca gatgtcagct ttcgcaatgg cggtctattt
     1981 caacggaatg accgaccgtg aaacagctga tctaacgatg gctatggttg aatcgggtga
     2041 tcaaatagat ttagcaaaga tagagggaac caaagtagat aaacacagta ccggtggggt
     2101 aggtgacacc acctccctgg tattagtacc cttagtttca gccgccggca tcccggtcgc
     2161 caagatgtcg ggccgggggt taggacatac cggtggtacc atcgataaat tagagtcgat
     2221 tcccggtttt aagaccgatt taagtatgga taaatttatt aataacgtca ataatattaa
     2281 agctgctatc gccggtcaga ccggtaattt aactccagct gataaacagc tttatgcctt
     2341 aagggatgta acagctacca tagaatctat cccgatgatt gccagcagta tcatgagtaa
     2401 aaagattgct ggtggggctg atggtatagt tcttgatgtc aaagttggct ccggtgcctt
     2461 catgaaaact ctgtccaatg caaaaaaatt agcagagatc atggtaaata taggtcaaca
     2521 tctaggccgt aatactattg ccgttcttac tgatatggaa cagcccttag gttatgcggt
     2581 cggcaataat cttgaggtta aagaagctat taatacttta aaaggtgagg ggccgagtga
     2641 cttaagagaa ctttgtttaa cacttggtgc aaatatgctg aaactggccg gtaaagtaga
     2701 tggttttaaa gaggggtatg aaattttagt agagaaatta accaagggag aagcctttaa
     2761 tcagtttaaa aagatagtag aaaatcaggg tggagatctc agttatattg agaaccctgc
     2821 taagtttaaa aaagcagacc atgtatttga agtaaaagcc aaaagtacag gctatttaaa
     2881 ccagcttaag gcccttgata tcggtttggc tgccatgtta gtgggagccg gccgtgagaa
     2941 aaagggagac gaaattttaa atgaagtagg cgttatcttg cataaaaaat atggtgaaca
     3001 tgtaaataag gatgaaataa ttgcaagcct gcattataat aaagatgatg ataacttaat
     3061 tgaagcaaaa gagaaattaa aaaattcatt tgtgattaaa gatgaagtac ctaagcaaag
     3121 agacttaatc tttgaaatag ttgattaaac tgtttgtaat gaacaaggag tgaaaataat
     3181 gagtaaattt gatggggaga gactcccggt agatatcttt caaattgacc aagaaaggat
     3241 gagacagggt tggtattctg atgtatactt tagaaatgta agtagaatat taactaagct
     3301 ctcagaagaa ggatatagat ttaaagatga agatattgga aatttagaag tggaaatgca
     3361 gatttttccg aggcggaagc ccttctgtat tttaggtggt attgatgaag ctctttccat
     3421 tttaaaaaaa tcaaccggtt atgaaaaaga agatggagag tttgtacaaa cttttgatga
     3481 gttagaagta atggcctgtc atgatggtga aaaaggctat tacaagggta atccggcccg
     3541 ggtagaccct gtaatgaaaa tcaggggcag gtatcgagac tttgcccacc tggagactcc
     3601 cattattgga tctttaaccg aggcttcccg gattgctacc aatgtctatc aggtaatgga
     3661 ggccgcccgg ggcaaagaca tattgttttt cccggcccga tttgcccatt ataaactaca
     3721 ggccttacat ggttacgctt attccttagg cgtcaatgcc tataatgaag actatgggca
     3781 gaacatgacc cgctatgtat cgaccgatga acagggtgcc tggtggggcg gtaaaggtgg
     3841 tggaacgatc agccactcct atatcgccac ctttttaggt aatacaacgg aagcaatgct
     3901 gcagttttca agactgatgc ccaaggatgt taaccggatt gccttggttg attttgataa
     3961 cgactgcgta aataccacgt taaaggtcct taaggccatg tttgataaat atattgaact
     4021 gaaagaagcc ggtaaagaag aagaggcttc aaaatataag ttgtttgggg taaggcctga
     4081 caactcttcc agtttaagag ataaaagtat cgagccctta ggagataaaa agctggataa
     4141 tggggttaat gcgagattgg tctttaagtt gagaaaagag atcgaccgcg cctataaaga
     4201 ctgggtctta aaagaaaaca gggtggaaga ggccaagcag ttctgtgaag atgtaaaaat
     4261 tatcgccacc ggtggcttta aacctaaaaa aataaatgaa tttgaagaat cccaagtacc
     4321 ggtagatatt tacggagtag gcagctggct tttatcgaac agttcagtgg aaggaactaa
     4381 aaatgacttt acagccgatg tagtccgggt taaaaaagat ggtgaatgga tagatatggc
     4441 gaaagttgga cgtaaggcct gtaataatga atgcatggaa ctggtcactg aatattaata
     4501 agtaggtttg gggttgatta tatggattta atcatctcac atgaaaatac agatatagac
     4561 ggtttagcag gcatgattgc tgcctctaaa ctttataccg aggccaaagt ggtcgtctct
     4621 gaaaacatga ctactttagc ccagcgtttt ttggccctgt ataaagacga gtttgagatt
     4681 ttccgctatg atgatataga ttatgaaggt attgaccgcg tgattgtgat cgatacccat
     4741 gagtacgata gattgggtaa gttgaccgag cttattaact gggatcaggt tgaggtgatc
     4801 gtctatgacc atcaccccca tcagaaactg gactggatag atatagattt atcggaaaat
     4861 gtcggttctg ctacctcgat aattgtgaac cgattaaaaa gggaaaatat taagttgaat
     4921 agatcagaag ctaccctgtt tgccctcggc atttcggccg atacaggtaa cttcatgcat
     4981 ctaaatacag aatcagaaga tctaattgcc tttgcttatc tattggatat gggggcaaat
     5041 aagaaggcga ttaacgaata ccttttagaa actctcgata aaaaccaaaa aagggttttt
     5101 gaactattaa ttaaacacag gaaggatatt aaaataaagg ataaagatat caccttattt
     5161 accctaaagt atccccgtta tgtgaccggc ctaaataatg tagtggaaaa aataaaactg
     5221 ctttatcagc tttccgtggt ctttgtctta gtagagatgg aaggcaaagt gataattatc
     5281 ggccgcagta gtgatgaaaa tgtaaacctg gaagaaatat tctctgagct aggtgggggc
     5341 ggacattccg gagctggttc agtatctatt aagaccgatt tagaaattgc ccgcaacaag
     5401 ttagaaacag ctattagaga taaaataaaa gtagcggtta aggtaaaaga agtgatgcat
     5461 gaaccaacca aagtacatga aagtactaaa ttgatcgatg ttaaaaagaa aatgaaaaaa
     5521 cacaatataa ccggtgtctt ggttactaac gatgaagata aggttaaggg tatatttacc
     5581 acccgcgact tacgccgctt aaatgatgaa gatgaacttt atcgagtccc ggtcaaggga
     5641 tacatgacga agaaactctt ttttatccag cccgatgata cgattcataa ggctcaggag
     5701 aggatggtta aaaaggatat cggccgcctc ccggttaaag atgataaggg agctgtagtc
     5761 gggatcatca ccagaaggga tatcttaaat cagtattatg aagaagaaac cgaaaatcaa
     5821 aatcaaaata gatatgtaag ttccctggtt tcaattacac cggagaaatt aaaaattccc
     5881 gataaacttg accaattatc agatgagatc agaaaacttt ttaaaaatat cggtaagata
     5941 gcccaggata aggactcccg catcttttta gtgggaggca tggtgcgcga cctcttattg
     6001 gagcaagaaa ataaggatct tgactttgta gtagatggga atattgaagg tttaatcaga
     6061 gaaattgctg ctaagtacga tgttaaatat aagtacaatg aaaactttca gaccgggagt
     6121 atggttatag ataaccagta tgatttagac tttgcggttt cccgcaatga actatatacc
     6181 caccccggtg ctttaccgaa agtacagaag gcagatattt ttgatgacct attccgccgg
     6241 gactttacaa tcaatgcttt agcgatcgcc ctccatccgg ctgagtacgg ttatttatat
     6301 gacttcttta aagcgcgcga agatgtggaa aataggatac tgaaagtcct gcatagattc
     6361 agctttttag acgaccccac ccggatcata agggggatca ggctggtatt gcagcttgat
     6421 ttagatattg aagaagagac caaggccttg atgcaggagg cgatcagatt aagtcgtttt
     6481 tccgatgtaa cctttccccg tatctataaa gagttaagat tcctgtttgc agccaacccg
     6541 ggcctgaaat ttattgaact tttaagggaa gtgcctttct taaggctgct atatgatgat
     6601 tacaaaatac ccgttaatat cgaagagaaa tggcagcggg ctgatagata ccttgcatat
     6661 ttcgaaaata ataattataa tgtaaaaaag tggccggtct atttttcctt gatcttaaaa
     6721 gatataccgc gttctattat tgaacgctgg tcacttaatt taaaggccaa aaatattata
     6781 ttatttaaac cggaagaata tgaaatagat aaaataacag acagtgccgg ccgggttgaa
     6841 ttgtataata atttaaagca gttcaccaat gaagaactag ttttactgtt ggcagaacat
     6901 gataatgaac agctacaaga aaaaatattt tactatttag aaaatatctc agatattaac
     6961 atcgcgattg atggtaatga tttaatcgaa cttggttatc aggaaggacc gataattagt
     7021 aaggcactgc gtttaattaa aagacaggtt ttaaaaggta acttacagag tagagacaaa
     7081 cagttaaagt atgcgaaaaa gttaaagaaa aaactggaga ggaagtaata aaagatgtca
     7141 aatataatgg aactaatttt ggtaataccg gtactcctat tatctttatc gatacacgag
     7201 tattcacacg gttatgtttc ttatttattg ggagatccca ctcctaaagc aaacggacgc
     7261 ttaaccttaa atccgattgc ccaccttgat ttagtaggaa gtttagtgtt gttaattact
     7321 aggaggatcg gctgggctaa accagttccg attgatccta gatactataa aaacccgcgc
     7381 aaagggttga tgttggtcgg tatagctggc cctggttcta atttgatttt ggcaacaatt
     7441 ttcgccctga tctttcgaac cgtgatggct tttgccagta ttaattcagt cggtgcgatg
     7501 ggcacccagg tcagcaacct tcaatatgtg gttttaaggt ttttaatcct ggcagttatc
     7561 gtaaacttga gtttaggatt ttttaaccta ctgccgatcc cccccttaga tgggtccaat
     7621 atattaaggg gacttatccc cagagactta gataagtatc taaataaatt acagggtccg
     7681 atcgggatga tattactctt ggttttagcc tataccggaa tcctgtgggg tatgatcagt
     7741 ccctttgtga acttcttttt aggtatatta atataataaa ttagataggt ggaaaatatg
     7801 gattatgaag ttcaactgga tgctttccag ggacctctgg agctcttata ccagctggtc
     7861 aagaaaaaca aaatagaaat cagccagatc tcgctggcca agataaccgg tgaataccta
     7921 gattatattg accaacatat catggatcta gaatatacca gtgaattttt ggtcattgca
     7981 gccgaactga ttgagataaa ggctaagtac ctattaccaa aaacagacga g
//
LOCUS       DATGCJ010000097         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_325276, whole genome shotgun sequence.
ACCESSION   DATGCJ010000097 DATGCJ010000000
VERSION     DATGCJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325276"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VKN64_04960"
     CDS             complement(<1..1047)
                     /locus_tag="VKN64_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HMA59625.1"
                     /translation="MTNNIPRPEYPRPQFKRDNWQNLNGNWNFAFDDENRGKKENWFN
                     KEKLGKEITVPFTYESKKSGIHDRSSHEYIWYQRKFKVPDKKKDKKVILHFAGSDYMT
                     KVWINGNYIGKHQGGYTSFSFEITNSIVYGNENNIVVKVEDQMSKSQPRGKQSWKAEN
                     FECWYSRTTGIWQTVWLEFVSKEIYLQDIQMTPDIDDKVIELEYYFSKEKLVEDYRIQ
                     TTIKYKDKLIDKFSYTIKDKQHKKMIKVENNDFRMKLWEPDNPNLYDINFKIRDDKNQ
                     IIDQVNSYFGMRKISIKDGQILLNNEPFYQKLILDQGYWPDTITTPPSDEAIKKDLKL
                     AKKMGFNGVRKHQKI"
     gene            complement(1072..1902)
                     /locus_tag="VKN64_04965"
     CDS             complement(1072..1902)
                     /locus_tag="VKN64_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59626.1"
                     /translation="MEDNNSPTTKVIRYSFSYLLAVLFIFPIIWMLFASLKPSGFHVA
                     NIIEWFLPPYSIVNYFDVIDRAPVIRWMLNSLVVASSVTFLVIIFDSLAAFALSVIDF
                     KYKNVVFVLFTVGLMVPTEANVVALFDIVNKLGLLNSYPGLIFPRVAIPLGIIILKEF
                     FDAIPRDLIDSARIDGCGLFRIYFSIFMPLSKAAIASIGIFTFIQAWNSFLWPFIAIM
                     SEEMFTLPVGVPTFTDAYTPDYSIPMTVNMIASTPAIIAFLLFQKQIIKGIKMTGIKG
                     "
     gene            complement(1904..2791)
                     /locus_tag="VKN64_04970"
     CDS             complement(1904..2791)
                     /locus_tag="VKN64_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59627.1"
                     /translation="MDAYKKLVKKYNFLKALPFILPFTIVYLIFLIFPIFRGLWISLH
                     RWTMVRKLDFVGLDNYLYMIKDPAFWESLGNTLLFVVLSTPAIVITGLVFALICNQKI
                     KGQLFLKISFFLPYVLSVAVVSSIMVYLFQPHSGLLNSVLQNLGVDQTIYWLGQDTLA
                     WFVIVFATLWWTVGFNMILYLAALQDIPKSFYEAAKVDGATKFQELWHITLPLLKPIT
                     WVVTLLQIIFSFKIFAQVWLITGGGPGTATRPIVQYIYETSFKENSLGYGASMAFTFF
                     IVLVAVSVFQIYFRRRKEG"
     gene            complement(2892..>3407)
                     /locus_tag="VKN64_04975"
     CDS             complement(2892..>3407)
                     /locus_tag="VKN64_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59628.1"
                     /translation="PLHSEYMENVNLFRNKKAATLLTGVWITGTLESTEGLNFGVIPM
                     PTMLDERASWADSHTLMLPYSPNQSPEKQKAALTFAKWVTDNGYLWAKAGHIPSKNTI
                     KENEKFQEMPYRMDYVEAADYVVFDKAGSHTWGIRSKMTTALSKVWKDEITPEEAVDM
                     AIENIDRLISR"
BASE COUNT         1109 a          617 c          520 g         1161 t
ORIGIN      
        1 aattttttga tgttttctca ctccattaaa acccatcttt ttggctaatt ttaaatcttt
       61 tttgatagct tcatcggaag gtggtgtagt tatagtatca ggccagtaac cctgatctaa
      121 aatcaatttt tgatagaatg gctcgttatt taataatatt tgaccatctt taattgaaat
      181 ttttctcatg ccaaaatagg aattaacttg atctataatc tggttcttat catcccttat
      241 tttaaagttt atatcataca gattaggatt atcaggttcc cataatttca tcctaaaatc
      301 attgttttcg acttttatca tctttttatg ttgtttgtct ttaatagtat aactaaattt
      361 atctattaat ttatccttat atttaattgt ggtctgtatt cgataatctt caactaattt
      421 ttctttggaa aagtaatatt ctaattcaat aaccttatca tctatatctg gggtcatttg
      481 tatatcttgt agatatatct ctttactaac aaattctagc cagacagtct gccagatccc
      541 ggttgtcctt gaataccaac actcaaaatt ttcggctttc cagctctgtt taccgcgagg
      601 ttggctttta ctcatttgat cttctacttt tactactata ttgttctcat ttccataaac
      661 aatagaattt gttatctcaa aagaaaagga agtatagcct ccttgatgtt ttccaatata
      721 attgccgttt atccagacct tagtcatata atcagagccg gcaaaatgta atatcacttt
      781 tttatctttt ttcttgtcag gaaccttaaa ttttctttga taccatatat attcatgtga
      841 tgatctgtca tggataccac tttttttact ttcataagta aaaggaactg tgatttcttt
      901 gccaagcttt tctttattaa accaattttc tttttttcct ctattttcat catcaaaagc
      961 aaagttccag tttccattta aattctgcca attatctctc ttaaactgag gtctggggta
     1021 ttcaggtctt ggtatattgt ttgtcatatt taagttccct ccttagcatg attaaccttt
     1081 gataccggtc atttttattc ccttgataat ctgtttctgg aataaaagga atgcaattat
     1141 agcaggggta gatgctatca tatttactgt catcggtatt gaataatcag gtgtataggc
     1201 gtctgtaaag gttggtactc ccactggtaa tgtgaacatt tcttctgaca taatagctat
     1261 aaagggccag aggaaactat tccaggcctg tatgaaagta aatataccaa tagaagcgat
     1321 agctgcttta gataagggca taaagataga aaaatagatc ctaaacagcc cacagccgtc
     1381 aatccgggca ctatctatta agtcccgcgg aatcgcatca aaaaattcct tcaagataat
     1441 tatgcctaaa ggtatcgcca cccgtggaaa gattaaaccc ggataggaat ttaataaacc
     1501 caacttattt actatatcaa ataaagctac cacatttgcc tcagttggaa ccattaaacc
     1561 tactgtaaat aatacaaaca caacattttt atatttaaaa tcaataaccg ataaggcaaa
     1621 ggctgctaat gaatcaaata ttattactaa aaatgttaca ctagaggcca caactaaact
     1681 attcaacatc catcttatta cgggtgctct gtcaataaca tcaaagtagt ttacaattga
     1741 atagggtggt aaaaaccatt caattatatt ggctacatga aaacctgatg gttttagaga
     1801 agcaaagagc atccaaataa tgggaaagat gaataataca gctaataaat aggaaaaaga
     1861 atatctaatt actttggtgg ttggagaatt attatcttcc atattaccct tcctttcttc
     1921 ggcggaaata tatttggaat acagaaacag ctacaaggac aatgaaaaag gtgaaggcca
     1981 ttgatgcacc ataacccaaa ctgttttctt taaaactagt ttcataaata tactgtacta
     2041 tcggtcttgt ggctgttcca ggtccaccac cagtaattaa ccaaacctgc gcaaatattt
     2101 taaaagaaaa aattatctgt agtaaagtta caacccaggt aataggtttt aaaagtggca
     2161 gtgtgatatg ccatagttct tgaaattttg tagcaccatc aaccttggct gcttcataaa
     2221 aggacttagg aatatcctgt aaggcagcta gatataatat catattaaat ccaactgtcc
     2281 accagagcgt agcgaaaact attacaaacc aggctaaagt atcctgacct aaccaataaa
     2341 tcgtttggtc tactcccaga ttttgtaata cactatttaa taatccactg tgcggttgaa
     2401 acagataaac catgatagaa gatactactg ctactgagag aacataaggt aagaaaaaag
     2461 aaatctttaa aaataattgc ccctttattt tttgattaca aataagtgca aagaccaagc
     2521 ccgtaatgac aatagcaggt gttgatagaa caacaaataa cagggtgttt cccaaggatt
     2581 cccaaaaagc tgggtcttta atcatatata aataattatc tagacctacg aaatctagtt
     2641 ttctgaccat tgtccagcgg tgtaaactaa tccaaagccc ccgaaagata ggaaatatta
     2701 aaaatattaa atatacgatc gtaaaaggta atataaaggg tagggccttt aaaaagttat
     2761 attttttcac aagcttctta taagcatcca aacatatacc tccttttcaa ctccgtatta
     2821 aaaaagaaaa agggggtggg tatataaaaa tatataccca cttatctatt ttatctttaa
     2881 ttagttagtt tctatctgct aattaaccta tcaatatttt caattgccat atcaactgct
     2941 tcttcaggag ttatttcatc tttccatact ttacttaagg ctgtagtcat cttagatcta
     3001 ataccccagg tatgtgaacc agctttatca aatactacat aatcagctgc ttctacataa
     3061 tccattctat aaggcatctc ttggaatttt tcattttctt ttattgtgtt tttacttgga
     3121 atatgtccag ctttagccca gagatatcca ttatcagtaa cccatttagc aaatgttaag
     3181 gctgcttttt gcttttctgg actttggtta ggtgaataag gtagcatcaa agtatgtgag
     3241 tcagcccaag aagcacgttc atctaacatg gtgggcattg gaataacacc aaagtttaat
     3301 ccttctgtac tttctaaggt acctgtgatc caaactcctg ttagaagggt agctgccttt
     3361 ttgtttctaa atagatttac attttccatg tactctgagt gaagtgg
//
LOCUS       DATGCJ010000098         7503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_235371, whole genome shotgun sequence.
ACCESSION   DATGCJ010000098 DATGCJ010000000
VERSION     DATGCJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235371"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VKN64_04980"
     CDS             complement(<1..348)
                     /locus_tag="VKN64_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA59629.1"
                     /translation="MKLNIIADSSCDLPEKYLKKIKLAPMTLKFKDEVVKDRVDISPS
                     DFYNKLKSINYSPGSAAPSAGDFLQHLKKDHDNFIIAISSALSSSYNNALVAKDLFLD
                     KVNDGVVHVFDTLN"
     gene            complement(564..1334)
                     /locus_tag="VKN64_04985"
     CDS             complement(564..1334)
                     /locus_tag="VKN64_04985"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMA59630.1"
                     /translation="METIKNYLYKRNEIIFAYLYGSFVKGTNNKESDLDIAIYIDENQ
                     KPASSKYGYRSYLISEIQAFKTKDIDLIILNESPNLIAFNILKEGKLLFSKSNKIHNA
                     FKKRIMDNYTDRNKYNLFKTKLNKLNRNIELLNQYKGIPFSNLNNNINKIWTIERGIQ
                     LCLKLMLDIGKYILLRKDIAGKDNEEILQKLIEQNIIPKRLGENIIEMEIMNLIENHN
                     SLEEKQLNYLLTNGIDDFEKIAFYINRYLELENEDIKN"
     gene            complement(1418..1741)
                     /locus_tag="VKN64_04990"
     CDS             complement(1418..1741)
                     /locus_tag="VKN64_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMA59631.1"
                     /translation="MYKGNAKLTRDKESNSNSKGIIVMAVLLVTLIIIISIFSYKALT
                     KKEIYYKDIEIKNGQTLWEIAESEFGNDIDVRKYVYKIKQINNLKNSNIHPGQIIKIP
                     VEREA"
     gene            1939..2595
                     /gene="lexA"
                     /locus_tag="VKN64_04995"
     CDS             1939..2595
                     /gene="lexA"
                     /locus_tag="VKN64_04995"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HMA59632.1"
                     /translation="MVDLESLSPRQREIYTYIVNEIDKKGYPPSVREIGKAVGLKSPA
                     SVHNHLKTLEELEFIRRDPSKPRAIEILNKSNKENEFADRFEKEMIHVPIVGKVTAGA
                     PILAEENIEDYFPIPTGFIKVRGKQLFMLEIEGESMINAGINDGDYVIAQQQNTANNG
                     EIVIALIDNRATVKRFYKKSDYVELRPENPDYDPILVKNVKLMGKVVGLFRNLEKSNF
                     "
     gene            complement(2592..3215)
                     /locus_tag="VKN64_05000"
     CDS             complement(2592..3215)
                     /locus_tag="VKN64_05000"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMA59633.1"
                     /translation="MIFNQNGKVNDSLFNYPLDDYEEFKNKALQCERCRLRESCTQVV
                     MGEGSLENKIMFIGEGPGASEDKKGRPFVGRAGRLLDKILKSVNIKRKDIYITNIVKC
                     RPPENRNPTENEMEACSPILKTEIKYIKPKVIVPLGSVALKYLVGPQRKITEDRGNWF
                     NKGELYFLPTFHPAYLLRNANMKRPVWNDFKKIKKAIERINELYDID"
     gene            complement(3233..3862)
                     /locus_tag="VKN64_05005"
     CDS             complement(3233..3862)
                     /locus_tag="VKN64_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59634.1"
                     /translation="MITILNHNKLILSIIIILLFSVAFTTNAAEEPCWKNLKNESVQE
                     IKQNPKDIKANYNHIISLVNLGEIKRAYKTIDKFEKDFNENDFIKQISPYFQELCKYP
                     DNILLLNYTAFYGVVIQEYDIAIKYFQRILKLTPNNYNIRNFLSASYYELEKFDLALK
                     EANKALKSKDNEFSHLLLGAIHYKNGNVLKAVSEFSKSGSLGREILNNN"
     gene            complement(3891..5060)
                     /locus_tag="VKN64_05010"
     CDS             complement(3891..5060)
                     /locus_tag="VKN64_05010"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HMA59635.1"
                     /translation="MENSNRIKNLPPYLFAEIDKMINNAKAEGMDVINFGIGDPDQPT
                     PENIVNKMKEAIQDPTTHSYPSYEGLVEFRTAVSRRYKKRFDVELDPATEVVSLIGSK
                     EGIAHLPLCYINPGDYSLVPDPGYPVYKTSILLADGNPIMMPLKEENDFLPELAAISE
                     EDAERAKIMFLNYPNNPTGAMASFEFFEEVIAFAKKYNIIIAHDAAYTDIYLDEEVPH
                     SILEVEGAKEVAIEFNSLSKPYNMTGWRIGWACGKEDVIESLGRLKTNIDSGIFEAIQ
                     YAGIEALYNSEDSIKEVTELYKERRDLLVEGLSELGWKVKPNKATFYLWIEVPENITS
                     SEFSKKLFKKTGVFFTPGIGWGDYGENFLRIALTVNQERIKEALQRIKENGFVYN"
     gene            complement(5063..5929)
                     /gene="dapF"
                     /locus_tag="VKN64_05015"
     CDS             complement(5063..5929)
                     /gene="dapF"
                     /locus_tag="VKN64_05015"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMA59636.1"
                     /translation="MKVKFLKMHGLGNDFIMINDLDVRLDNYSKLAVNLCDRHFGIGA
                     DGIILIQQSDLTHADFKMRIFNPDGSEAEMCGNGIRCFAHYLHINDLTEKKKLSIETL
                     AGLIKPEIIDYGPHESTVKVNMGEPAYSLESLDINKELLDDDIDKLWDYPLIIEGKEY
                     ELNGASMGNPHMVIYVDDLKDINLKKLGPIIEENPLFKKGTNVEFVKIVSKNELDIKV
                     WERGAGITLACGTGACATAAVSIDQDRVNNNVKINLPGGPLKISKNENNEMMMTGPST
                     FVFKGEIMTGGI"
     gene            complement(5919..6176)
                     /gene="hfq"
                     /locus_tag="VKN64_05020"
     CDS             complement(5919..6176)
                     /gene="hfq"
                     /locus_tag="VKN64_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HMA59637.1"
                     /translation="MKNKVNIQDTILNHVRKQSIGVKIYLMNGVQLKGKVIGFDDFTI
                     LLEVENDLKVIYKHAVSTIEPQENIKDVIFNNYRDKKKNES"
     gene            complement(6196..7140)
                     /gene="miaA"
                     /locus_tag="VKN64_05025"
     CDS             complement(6196..7140)
                     /gene="miaA"
                     /locus_tag="VKN64_05025"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMA59638.1"
                     /translation="MANKYDKVVTVLGPTAAGKTDLSLKIAEEFKAEIISADSMQIYK
                     EMDIGTAKVSQNIQAKIKHHQIDIINPDEDYSVADYQEDVDPIIADIFNRNKLPLMVG
                     GTGLYIKAVLEGFSLPDMEPDEELRAKLRQEAKEKGNQYVHDKLKEIDPKLGQKLHPN
                     DLRRVIRGIEIYKLTGHNKSYYKKKQEEAPPRYQSIKIGLTRNRQELYDRINKRVELM
                     IEEGLVEEAKKIFNSYNLDKKSTARQAIGYKELFKYFKGKYDLEEAVRRIKRNTRHYA
                     KRQLSFFKRDDEIKWYNLTEISKKNLFKKVLEYLHNNL"
     gene            complement(7140..>7503)
                     /locus_tag="VKN64_05030"
     CDS             complement(7140..>7503)
                     /locus_tag="VKN64_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59639.1"
                     /translation="NNQSTFSNLGIEFEQFGPKSIIVNAIPTYLKEKNQKSLVRDIID
                     EIVKQNKNVEKSQLIEEALKYIACRSAVKAGEQLTNNQITYIVNNLFKLENYDRCPHG
                     RPILLILSKEEIDKEIGR"
BASE COUNT         2305 a         1286 c         1050 g         2862 t
ORIGIN      
        1 attgagagta tcgaagacat ggaccacacc atcatttact ttatccaaaa acagatcttt
       61 agcgactaaa gcattattat agctgctgct taaagcagag gaaattgcaa tgataaaatt
      121 atcatgatct ttttttagat gctgaagaaa atcaccggcg gaaggagcag ctgaaccagg
      181 actgtaattt attgatttta atttgttata aaaatcggac ggactaatat caacacggtc
      241 cttaacaact tcatctttaa attttaaagt cattggagct aattttattt tctttaaata
      301 tttttctggt agatcacaag aactatctgc aattatattt aatttcaaat tcaaaacccc
      361 caaatatatt tttaaataaa aaaagaccct atttcattat tataaaataa aaatagaggt
      421 ttttaaagta ttttggccta tatttaggta aaaattaggt ataatttcgt aaaataaaca
      481 ttttacgaaa taaataaaaa atataaagat agcttgccct aactttcaca tggtcttctc
      541 gtttatattt tggtctatgg agattaattt ttaatatctt cattttctaa ttctaaatat
      601 ctattaatat aaaaagcaat cttctcaaaa tcatcaatac cattcgtcaa taaataattg
      661 agctgctttt cttctagact gttatgattt tctatgagat tcataatttc catttcaata
      721 atattttctc ccaatctttt gggtattata ttttgttcaa ttaacttttg taaaatctct
      781 tcattatctt tcccagctat atcttttctt aataaaatat atttacctat atctaacatt
      841 agtttaagac aaagttgaat tcctctctct attgtccata tcttattaat attattattt
      901 aaattactga agggtattcc tttgtattga tttaaaagtt caatatttct atttagctta
      961 tttaatttag ttttaaataa gttgtattta tttctatcag tataattgtc cattattctt
     1021 tttttgaaag cattatgtat tttattagat ttagaaaata ataattttcc ttctttaagt
     1081 atattgaaag ctattaaatt tggactttca ttaaggataa tcaaatcaat atctttcgtt
     1141 ttgaaggcct gtatttcaga gattagataa cttctataac catatttact actggcaggc
     1201 ttttgatttt catcaatata tattgctata tctaaatcgc tttctttatt atttgtgcct
     1261 tttacaaaag atccatatag ataagcaaat attatttcat tccttttata taaataattt
     1321 tttattgttt ccaaacttca cacctcatta atattctatc aaaaactatt aaaaaaagaa
     1381 atgtttgacc ttatttgaag cgattcgaac atacatacta tgcttctcgt tcaactggaa
     1441 tttttattat ttgccccgga tgaatattgg aatttttcag attattaatc tgctttattt
     1501 tataaacata ttttctaaca tcaatatcat taccgaactc actttcggct atttcccaga
     1561 gtgtttgccc atttttaatt tcaatatctt tataataaat ttcttttttt gtgagagctt
     1621 tataagaaaa tatactgata ataattatta gagtaaccaa taagactgcc ataacaataa
     1681 tcccctttga atttgagtta ctttctttat ctcttgttag ctttgcatta cctttataca
     1741 ttttatccgc ctccaataca tatgttctta tttaagatta taccaaaaca tatgtttgaa
     1801 gtcaaataat attgtcagat attagaacga ttgtttgctt tatgggggtt tgtgtgttat
     1861 aatttaagca gtaatagtaa attgtcataa aattttacat ttatatatat ttaaaattaa
     1921 atttctggag ggaaaaatat ggttgatctt gaaagtttaa gcccacgaca gagagaaatt
     1981 tatacttata ttgtaaatga gatagataaa aaaggatatc caccctctgt tcgtgaaatt
     2041 ggaaaagccg tggggctaaa atcacctgct tctgtacata atcatttaaa aacattggaa
     2101 gaattagagt ttatcaggcg agatccttcc aaaccaagag caattgaaat tttaaataaa
     2161 agtaataagg aaaatgaatt tgcagataga tttgagaaag aaatgataca tgtaccaatt
     2221 gtagggaaag ttacagctgg tgctcctatt ttagcagaag aaaatattga agactacttt
     2281 cctattccga caggctttat caaggtgagg ggaaaacaat tatttatgct ggaaattgaa
     2341 ggggaaagta tgatcaatgc tggtattaat gacggagatt atgtcattgc ccaacagcaa
     2401 aacactgcta acaatggaga aatagtaata gccctgattg ataacagggc tacagtaaaa
     2461 agattttata aaaaaagcga ttatgtagag ttaagaccgg aaaatccgga ctatgatcct
     2521 atattagtta aaaatgtaaa attaatgggt aaagttgttg gtttattccg taatttagaa
     2581 aaaagtaatt tttaatctat atcatagagc tcatttattc tctcgattgc ttttttaatc
     2641 tttttaaaat cattccagac cggtcttttc atattggcat ttcttagaag ataggcaggg
     2701 tgaaaagtag gtaaaaaata taattcacct ttattaaacc agtttcccct gtcttcagtt
     2761 atttttctct gtggtccaac taaatatttt aaagcaaccg aacctaaagg tacgattact
     2821 ttaggtttta tgtattttat ctcggttttt aaaatgggtg aacaggcttc catctcattt
     2881 tcagttggat tcctattttc aggtggtcgg cattttacaa tattggttat atagatatct
     2941 tttcttttaa tgttgacaga tttaagtatt ttatcaagta accggccggc tcttcctaca
     3001 aagggtcttc cctttttatc ttcacttgca cccggccctt ctccgataaa cataattttg
     3061 ttttcaagtg agccttctcc catcaccacc tgggtacagc tctctcttaa tctacatctc
     3121 tcacattgaa gagccttatt tttaaattct tcgtagtcat caagcggata attaaaaaga
     3181 ctatcattta cctttccatt ttgattaaat atcataacta agccgctcct atttaattat
     3241 tatttaaaat ttctctacct aaacttcctg atttggaaaa ttcacttacc gcctttaata
     3301 cattgccatt tttatagtga atagctccta aaagaagatg tgagaattca ttatcctttg
     3361 attttaaagc cttattggct tcctttaatg cgagatcaaa tttttctaat tcgtaataac
     3421 tggctgagag aaagttccgt atattatagt tgttcggtgt taatttcaat attcgctgga
     3481 agtattttat tgctatgtca tattcctgga taactactcc ataaaaagca gtgtagttta
     3541 aaagcaagat attgtcggga tatttacata attcctgaaa ataaggagat atctgcttta
     3601 taaaatcatt ttcattaaaa tctttttcaa atttatcgat agttttatag gctcttttga
     3661 tttctcccag atttactaat gaaataatgt gattataatt agcttttata tccttggggt
     3721 tttgtttaat ttcttgaaca ctttcatttt taagattttt ccaacagggt tcttccgctg
     3781 cattagtagt aaaagcaact gaaaacaata atataattat tattgataaa attaatttat
     3841 tgtgatttaa aattgtaatc aacctcttct aatttgaaat taaactcatg ctagttatag
     3901 acaaacccat tttctttaat tctctgtaaa gcttctttta ttctttcctg attaactgtt
     3961 aaagcaatcc gcaagaagtt ttctccatag tctccccagc cgataccagg tgtaaagaat
     4021 acgccggtct ttttaaataa ttttttagag aattcagaag aagtaatatt ttctggcact
     4081 tctatccata gatagaaagt tgctttattg ggtttaacct tccaaccaag ttcagataaa
     4141 ccttctacca gtaggtctct tctttcttta tatagttcag taacttcttt gatactgtct
     4201 tcagaattat ataatgcttc tatgcccgca tattgaattg cttcaaatat acctgaatct
     4261 atatttgttt ttaatcgacc cagggactct ataacatctt cttttccaca agcccaacca
     4321 attcgccagc ctgtcatgtt atagggtttt gaaagggaat tgaattcgat cgctacttct
     4381 ttggcgcctt caacttctaa tatactatga ggaacttctt catccagata aatatcagta
     4441 taagctgcat catgtgctat tattatatta tattttttgg caaaagcaat aacttcttcg
     4501 aagaattcaa aacttgccat tgcaccggtc gggttattag gataatttaa aaacattatt
     4561 ttagctcttt ccgcatcttc ttctgaaata gcagcaagtt cgggtaagaa atcattttct
     4621 tcttttaaag gcatcatgat cgggttcccg tctgccagta atatacttgt tttatagact
     4681 ggatagcccg gatcaggcac taaggaataa tcacccggat taatataaca aagtggtaaa
     4741 tgggcaattc cttctttgga accgattaat gaaactactt cggtggcggg atctaactcc
     4801 acatcaaatc tctttttata tcttctgctt accgctgttc tgaactcaac taaaccttca
     4861 taagaaggat aactatgagt tgttggatcc tgtatagctt ctttcatctt atttacaata
     4921 ttttcaggtg taggctggtc gggatcgccg ataccaaagt taataacatc cattccttct
     4981 gctttggcat tatttatcat cttgtctatt tcagcaaata aatatggtgg taagttttta
     5041 atacgatttg aattttccat aattagattc ctcctgtcat tatttcacct ttaaatacaa
     5101 aggtggaagg acccgtcatc atcatttcat tattttcatt tttagatatt ttaagtggtc
     5161 ctcctggtag gttaattttt acattattat taactctatc ttgatcaatg ctgactgctg
     5221 cagttgcaca ggcaccagta ccacaggcta aagtgatacc tgctcctctt tcccaaactt
     5281 taatatctaa ttcatttttg cttacaattt ttacaaattc aacattggtg ccctttttaa
     5341 ataatggatt ttcttcaata ataggcccta atttttttaa gttaatatct tttaaatcat
     5401 cgacatagat taccatatgg ggattaccca ttgaagcccc atttaactca tattctttac
     5461 cttcaattat aaggggataa tcccataact tgtcaatatc atcatctaat agttctttgt
     5521 taatgtctaa actttctaaa gaataggctg gttctcccat gtttaccttg acagtacttt
     5581 catggggacc ataatcaatt atttcgggtt tgattaatcc tgctaaagtt tctatagata
     5641 acttcttttt ttcagttaaa tcattgatat gtaagtaatg agcaaaacat cttataccat
     5701 taccacacat ttcagcttca ctgccatccg gattaaagat tctcatttta aaatcagcat
     5761 gagtaagatc ggactgttgg attaatatta ttccatctgc cccaattccg aaatgcctat
     5821 cacataaatt gactgccagt ttagagtaat tatctaacct tacgtccagg tcatttatca
     5881 ttataaaatc attacctaaa ccgtgcattt ttagaaactt aactttcatt cttcttctta
     5941 tcccggtaat tattgaatat tacatcttta atattttctt gaggttcaat tgtagaaact
     6001 gcatgtttat agattacttt cagatcattt tctacttcta aaagaatagt aaagtcatca
     6061 aaaccaatta cttttccttt taattgaact ccgttcatta aatagatttt aacaccaata
     6121 ctttgttttc ttacgtggtt taaaattgta tcctggatat tcaccttatt cttcatacca
     6181 attaatcccc cttatttaaa gattgttgtg caggtattct aatacctttt taaaaagatt
     6241 ttttttactt atttccgtga gattatacca tttaatttca tcatctcttt taaagaaact
     6301 caactgtctt tttgcataat gcctggtgtt tcttttaatg cgtctaactg cttcctcaag
     6361 atcatatttt cccttaaaat atttaaaaag ctctttatag cctattgcct gacgggcagt
     6421 agacttttta tcaagattat aggaattaaa gatcttttta gcctcttcaa ctaaaccctc
     6481 ttcaatcatc agttctactc ttttgtttat tctatcatat agttcttgtc tatttctagt
     6541 taaaccgatt ttaattgact gatagcgggg aggggcttcc tcttgttttt tcttataata
     6601 actcttatta tgaccggtca gcttatatat ttcaatacct ctgatcaccc tccgtagatc
     6661 attgggatgt aatttttggc ccaacttagg atcgatttcc tttaatttat catgtacata
     6721 ttgattgcct ttttcttttg cttcttgtct aagtttggcg cgtagttctt catcgggctc
     6781 catatccggt aaagaaaagc cttccaagac tgctttaata taaaggccgg ttcctcctac
     6841 catcaagggc agtttattcc tattaaaaat atctgcaatg attggatcaa catcttcttg
     6901 gtaatcagcg acagaataat cttcatctgg gttaattata tcaatttgat ggtgtttaat
     6961 tttagcttga atattctgag aaactttggc tgtaccgata tccatctctt tatagatctg
     7021 catggaatcg gccgagataa tttctgcctt aaattcttca gcaattttta aagaaagatc
     7081 tgttttaccg gccgccgtag gtcctaaaac agttaccact ttatcatatt tattagccat
     7141 tacctaccaa tctccttatc gatctcttct ttggatagga taagtagtat tggtctgccg
     7201 tgtggacacc tatcgtaatt ttctaacttg aaaaggttgt ttacaatata agttatttga
     7261 ttattggtca actgttcacc ggcttttaca gcactgcggc aggctatata ctttaaagcc
     7321 tcttcaatca attgggattt ttccacattt ttattctgct ttacaatttc atctatgata
     7381 tctctgacca aagatttttg atttttttct ttcagataag tgggaattgc atttacaatg
     7441 atgctttttg ggccgaactg ttcgaactct atgcctaagt tactaaaagt agattggttg
     7501 ttc
//
LOCUS       DATGCJ010000099         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_146504, whole genome shotgun sequence.
ACCESSION   DATGCJ010000099 DATGCJ010000000
VERSION     DATGCJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146504"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..555
                     /locus_tag="VKN64_05035"
     CDS             202..555
                     /locus_tag="VKN64_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMA59640.1"
                     /translation="MSEKDFWEEREEMNKKIYEADNIEIKRFFNLDTNVYKEGALSTK
                     NKEMLGLASSMVLRCDDCIRYHIDRCYEEGVSKDEFFELFSVVLIVGGSIVIPHLRRA
                     VKRLEELFEGEGEVK"
     gene            552..1421
                     /gene="rnhA"
                     /locus_tag="VKN64_05040"
     CDS             552..1421
                     /gene="rnhA"
                     /locus_tag="VKN64_05040"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HMA59641.1"
                     /translation="MIQFEPIGKIITPYKERKNIPRQPDMDAEGEFILEIDKNLQEGL
                     EGLEKYDYIIVVFYLDKIEDKDPELIINPTYSTSNKMRGLFATRTPNRPNKIGISTVG
                     LKYIEDNKVYISGIDAVNGTPILDIKPYYRSLDSKDEYYNYNKDEIIIYTDGACYKNP
                     GPGGYAAVILDEDKEIEIMGYEPDTTNNRMELRAVIEALKVVRKNKKIVLYSDSNYVV
                     KGINEWIKNWKKRGWRTSSDKPVKNLDLWKKLDKLFDKKNVKLKKVAGHSGNEYNEKV
                     DSLAKEEIEKNIK"
     gene            1439..>2178
                     /locus_tag="VKN64_05045"
     CDS             1439..>2178
                     /locus_tag="VKN64_05045"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMA59642.1"
                     /translation="MLSYKEKRKEAKDYILQRVDIKPEIGLILGSGLGEYSEQLENQT
                     KLKYDDIPGMPVSTVEGHAGEFVFGEKHGKKIVAMNGRVHYYEGYSMKEIAFPVWVMK
                     ELGIKKMIVTNAAGGLNTTFRPGDLMIVTDHLNFMGSNPLIGENDEEIGPRFPAMTDA
                     HTAHMVDIAEKAADRANIHIMKGVYLAITGPVYETYAMSKFQRLIGADAVGMSTVPEL
                     IAGTHAGLENLAISCITDVVKGKDEESLT"
BASE COUNT          851 a          257 c          466 g          604 t
ORIGIN      
        1 aaaaaagaag aactaagtaa aaaattagat gaattttatg aggaaagaca agaggaatca
       61 tgatatttaa caaacttata accaaaagat ttctatattt gcaaataacg aataatcatt
      121 atatacttaa aaaaaaatat cggaaatgct atttagataa tgtaaataat attgaaatat
      181 aggccagagg aggcagaaat tgtgagtgaa aaagattttt gggaagaaag agaagaaatg
      241 aataaaaaaa tatatgaagc ggataatata gagataaaaa ggttttttaa tttggataca
      301 aatgtatata aggaaggtgc cctcagtaca aaaaataaag agatgttagg tttagcttct
      361 tctatggttt taagatgtga tgactgtata agataccata ttgaccgttg ttatgaagaa
      421 ggagttagta aagatgaatt tttcgaacta tttagtgtgg ttttaatagt cggaggttca
      481 attgtgatcc ctcatctgag aagggctgtt aaacgactag aagaactatt tgaaggtgag
      541 ggggaggtta aatgattcaa tttgaaccga ttgggaagat aattactccc tataaagagc
      601 gtaaaaatat tccccggcag cccgatatgg atgctgaggg agaatttatt ttagaaatag
      661 acaaaaattt acaagaaggg cttgaaggac tagagaagta tgattatata attgtggtat
      721 tttatcttga taaaatagaa gataaagacc ccgaactaat aatcaatccc acttattcca
      781 caagtaataa gatgagggga ttatttgcta ctagaactcc taatcgaccc aataaaattg
      841 ggatcagtac agttggactt aaatatatcg aagataataa agtatatatt tctggtattg
      901 atgcagtaaa tgggactccc atcttagata taaaaccata ttatagatcc ttagattcaa
      961 aagatgaata ttataattac aataaagacg agataataat ttataccgat ggggcctgtt
     1021 ataaaaatcc agggccgggt ggttatgcag cagttatctt agatgaagat aaagaaattg
     1081 agattatggg ttatgaaccc gataccacta acaacagaat ggaattaagg gcagttatcg
     1141 aagccttaaa ggtggtaaga aagaacaaaa agatagtact ttattctgat tcaaactatg
     1201 tagttaaagg tataaatgaa tggattaaaa actggaaaaa acgaggttgg cgtactagta
     1261 gtgataaacc ggttaaaaat ctagatttat ggaaaaagct agacaaatta tttgataaaa
     1321 agaatgtcaa gctaaaaaag gttgcaggac attctggtaa tgaatataat gaaaaagtag
     1381 atagtttagc aaaagaagaa atagaaaaga atataaaata acttggggga atttattaat
     1441 gcttagttat aaagaaaaga ggaaagaagc gaaagactat attcttcaaa gagttgacat
     1501 caaaccggag ataggactaa ttttaggttc tggcctggga gaatacagtg agcagttaga
     1561 aaatcaaaca aaattaaaat atgatgatat tcctgggatg cctgtttcaa cggtagaagg
     1621 tcatgctggt gaatttgtat ttggagaaaa acatggtaaa aagatagtag ctatgaacgg
     1681 ccgagtacat tattatgaag gatatagtat gaaagagatc gctttcccag tttgggtcat
     1741 gaaagaactg ggcattaaaa aaatgatcgt tactaatgcg gccggaggtt tgaataccac
     1801 ctttagaccc ggagatttaa tgattgtgac agaccatctt aattttatgg gttctaatcc
     1861 gcttattggt gaaaatgatg aggaaatagg accacgcttt cccgctatga cggatgcaca
     1921 tactgctcat atggtggata tagcagaaaa ggcagctgac agagctaata ttcatattat
     1981 gaagggtgtt tatttagcta taacaggacc agtttatgaa acttatgcaa tgagtaaatt
     2041 tcaaagatta atcggagcag atgcggtggg aatgtcaacc gtacctgagc taattgcagg
     2101 tacccatgca ggattagaaa acttagcaat atcttgtatt acagatgttg taaaaggaaa
     2161 agatgaagaa tcattaac
//
LOCUS       DATGCJ010000100         7639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_12247, whole genome shotgun sequence.
ACCESSION   DATGCJ010000100 DATGCJ010000000
VERSION     DATGCJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12247"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VKN64_05050"
     CDS             <1..833
                     /locus_tag="VKN64_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59643.1"
                     /translation="FFVSYNGRDAALARDSLIKNFLDRYYPSDRDIVPEEAGSNFMDN
                     SKITGTYYTNRSNFTDYQSVVRIITQTNIRLTQAGNLTYNFMGENHQLKQIAPRVFYD
                     KDLGNKLYVPEGEEINRLFTNGPNGLLKAQWYETLNFNLIFLIGYALFGIVFSIVLIK
                     SLFKRYIRNKYILEKITVVIFTVAAIGFFIAIGIFLANTHPLYNVPYLFLEVTPAFDN
                     ILLFLWIIPVLAVILVVMNVRIWFKGRWNVIQKGIYTIYTIWSIGITWWFYYWNLLQL
                     "
     gene            864..1700
                     /locus_tag="VKN64_05055"
     CDS             864..1700
                     /locus_tag="VKN64_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA59644.1"
                     /translation="MELSVLVDNNTIIDHYFKGEPGLSYYIKEGETHILFDVGYSDIF
                     IENAHQMGLDLNQVDYLIFSHGHIDHTGGLDPLIKHYFETKSESDKGYPEIVAHPDCF
                     HDKYYGNNTSIGVNVSEKVINQLFKVTKSKEAIWITEKLVYLGEVERTNDFEAKEPIG
                     YQDKIAEGHEDYIRDDTALAYLSEEGLVIITGCSHAGICNIVEQAKKVTGIDNVVDII
                     GGFHLMEPDKKQLSKTKEYLEKLNLMQLHACHCTDLNSRVHLAEISDLKEVGVGMRLE
                     YK"
     gene            1741..2106
                     /locus_tag="VKN64_05060"
     CDS             1741..2106
                     /locus_tag="VKN64_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59645.1"
                     /translation="MRLIFPSYYSMIVGLLMISQWLFFILSGNLPEFSITPIEISIHI
                     FVEILTALALLVSGYRLLNKKKYSIKLNILAQGMLIYAIINSSGYFAQLGQWIFVVMF
                     AVLLILSVVSLKQLMKGVK"
     gene            2107..2919
                     /locus_tag="VKN64_05065"
     CDS             2107..2919
                     /locus_tag="VKN64_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59646.1"
                     /translation="MNEEQFNIPDIILYIILGLLIFASLSGIFREGLYQDNLFVQSAW
                     YGTDLITFILAMPIFVISIILYKKKTLFGLLLIFAMLFYSLYNYAFYLFSAALNDMYL
                     VYVAIFALSFYSILLLVRKINIEKVAASYKESTPTKLIGGYMVIVGILLGVFHIGLSL
                     SYVFTDQVPAIVTNVAHPTNVIAALDLSLVVPIALIGGISLLKKQAWGYVLAVVWNFK
                     GFIYMTALTSASVVAYMNGATTSLAELFLWVPIAVGCIIMAYISLKNIETID"
     gene            2968..3723
                     /locus_tag="VKN64_05070"
     CDS             2968..3723
                     /locus_tag="VKN64_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMA59647.1"
                     /translation="MKRVEFKNKRGLTLIGDLYKAKSDHIVIMAHGFMNDRSSQGRFE
                     QVAERLNEASYNILKFDFSGCGESDDDIIELDKEVEDLLSAVEFVKEKGFKNIALFGN
                     SLGGLVCLKAYSSDISTMVLTGPITDSMNYDWEKFYSKEKMEELKEKGYLTMGNQKTG
                     IRKISQDMLDAFSEIDQEEILKGIDCPVLIIHGDRGEEEKELLDRSKKALKMLPNSSK
                     LKVVEDAAHGIRDQYHIVVELTVDWILNNFEIE"
     gene            3866..4348
                     /locus_tag="VKN64_05075"
     CDS             3866..4348
                     /locus_tag="VKN64_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59648.1"
                     /translation="MKKKLLGYVGRFTLLHVITYTVAGVIFMNVFNYAEVFETSQVFA
                     HFRSVDSPIMQAAPFLQIIRGGFFALILYPFYEVIVNHKRGWLVLFGVLWGLTYIGSV
                     EATLGSIEGLIYTKATLVEHFVGTPEVTIQMLVFSWLFVKWEKRSIKKEKTRQKSKNP
                     "
     gene            4428..5084
                     /locus_tag="VKN64_05080"
     CDS             4428..5084
                     /locus_tag="VKN64_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA59649.1"
                     /translation="MTGEKIKLLIADDHPFFRQGISLFLDDTSEIEIKAEADNGKIAL
                     DTIKKDNDFDVILMDLQMPEMDGIEATAQIKSDYPSIKVLILTSFNSWSKVYEALKAG
                     ADGYIMKDSKPHELVAAIKAVNTGGVYYGAEVAEQLLKRVNQTSSEAENEELIEPLTD
                     RELEVLALLGRGLSNQEIADQLVVSIKTVKTHVSNILAKLEVDSRTQAAVFAIRKGLI
                     "
     gene            5085..6266
                     /locus_tag="VKN64_05085"
     CDS             5085..6266
                     /locus_tag="VKN64_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMA59650.1"
                     /translation="MVVTINKNEISSLEKYKNIHDNKQASYWQNLKVSSLFGLLFGIV
                     FLIVTLITRDYTGWDIYIIPILCAVISGMAGLLATFLERYFLNRGLENALIRKTIIFS
                     VVITLTITLTIVVFTQNSVANFFETLRNNMIWGIVFGVIFAVVIAALEYYNWKVEQKM
                     AILELENKYLEDLAAKDTMLKEATKNLLVSKERNKMARELHDSISQDIHGLSYGLSSL
                     KNQMKTLDIEDEKVFKILNHLEKTVENTSEELKYMIEELKPAALEKNSLKDALLTHLD
                     LFEARQDIEVKKEIDDIKELSPEQEYSVYRIVQEALANIQKHAKADKVIVYFKKLPDN
                     NFGKAKLTIEDNGQGFDSDKIKKGNGLENMELRSKQIGGNFQVKSKTGAGTQIKVEFN
                     Y"
     gene            6284..7153
                     /locus_tag="VKN64_05090"
     CDS             6284..7153
                     /locus_tag="VKN64_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HMA59651.1"
                     /translation="MDFLKYVKNNKITLLDGAMGTELKKRDVMGNFKNNIENQSIVKE
                     IHESYIKAGSNAIITNTFSMNSLYLDSKDADVGVELINKKGVEIAQEAAGDDIFVLGD
                     ISSTGKLLKPYGEYEENAFIDVYKQKAEILAENGVDAFIIETMVDLREAICALKACKQ
                     VSELPVIVSISFDRVEQGGRTVMGNTAREVVENLSKEKADIIGTNCGSLSPKQMAEIV
                     KMMRTETDLPIMVEPNAGQPKLKGEETVYDMQPNDFTAGILACIEAGANIVGGCCGTS
                     PAHIDDLNKKLFG"
     gene            complement(7228..7398)
                     /locus_tag="VKN64_05095"
     CDS             complement(7228..7398)
                     /locus_tag="VKN64_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HMA59652.1"
                     /translation="MGKDKRFELATSQGITPNTYQVIVDKETGVNYFYVRGGGLTVLV
                     DADGKPIVTKDN"
BASE COUNT         2662 a         1074 c         1520 g         2383 t
ORIGIN      
        1 gttttttcgt atcctataac ggtagagatg ctgctttagc cagggattcc ttaataaaga
       61 actttttaga tagatactat ccatcagata gagatatcgt tcctgaagaa gcaggcagta
      121 actttatgga caattctaaa ataaccggta cttattatac gaatagaagt aattttacag
      181 attatcaatc tgttgtcagg attatcacac agactaatat tagattaact caggccggca
      241 atcttactta taactttatg ggggaaaacc atcaacttaa acagatagct cctagagttt
      301 tttatgataa agaccttggt aataaactat atgtgccgga aggtgaagaa atcaaccgac
      361 tttttacaaa cggcccgaat ggtttgttaa aggctcaatg gtatgaaaca cttaacttta
      421 acctaatatt tctaataggt tatgcgctgt ttggaattgt atttagtatt gtcttaatca
      481 aatcattgtt taaaagatat attagaaata agtatatttt agagaagatc acggtagtta
      541 tatttacagt ggccgcaatc ggtttcttca tagcgatcgg aatattttta gcaaacactc
      601 atcccttata taatgtaccg tatctattct tagaggtgac acctgctttc gataatattt
      661 tgcttttcct ctggataata ccagttttag ctgtgattct tgtcgtaatg aatgtgagaa
      721 tctggtttaa agggagatgg aatgtaattc agaaaggtat ctataccatt tataccatct
      781 ggtcaatagg tataacttgg tggttttatt actggaactt acttcaatta taaagaggga
      841 acatatttgg aggtgaaata ttgatggaat tatcagtctt agttgataac aacaccataa
      901 tagatcatta ttttaaaggg gaacccggcc tctcctatta tataaaagaa ggggagaccc
      961 atatcttatt tgatgttggt tattccgata tatttattga aaatgcccat cagatgggcc
     1021 tggacttaaa tcaggtggat tatttaatat tttcacacgg acatattgat catacaggtg
     1081 gacttgatcc attaatcaaa cattactttg aaactaaaag tgaatccgat aaaggatatc
     1141 cagaaatagt tgctcatccc gactgcttcc atgacaaata ctatggcaac aatacttcaa
     1201 ttggcgttaa tgtgtcggaa aaggttatca accagctttt caaggtcacg aagagtaaag
     1261 aagcaatttg gattactgaa aaattagtat atctaggtga ggttgaaaga actaatgatt
     1321 tcgaagcaaa agaacctatc ggctatcagg ataaaatagc ggaaggccat gaagattata
     1381 tcagggatga tacagcctta gcttatttat cagaagaagg attagtaata ataaccggtt
     1441 gttcccatgc tggaatctgt aatattgttg aacaggcaaa aaaggtaacc ggtattgata
     1501 atgttgtgga tattatcggc ggttttcact tgatggaacc ggataaaaaa caattaagta
     1561 agacaaaaga gtatttagaa aaactaaatt taatgcaatt acatgcctgc cactgtactg
     1621 atttaaatag cagagttcat ctagcagaga tatctgattt aaaagaggtc ggggtgggta
     1681 tgaggttaga atataaataa aaagaagaat tttaaagttt agattatttt gaagggagct
     1741 ttgcgattga tatttccatc ctattactcg atgattgtag gcttattaat gataagccag
     1801 tggttatttt ttattttaag cggtaattta ccggagttca gtataactcc aattgagatc
     1861 tcaatccata tttttgttga gatattaaca gccttagctt tattggttag cggctatcgt
     1921 ttattaaata aaaagaaata ttcaattaaa ttgaatatat tagcccaggg catgttaata
     1981 tatgcaatca ttaatagttc gggctacttt gcgcaattag gtcaatggat atttgttgtt
     2041 atgtttgcgg tccttttaat tttatctgta gtgagtctaa aacaactcat gaagggagta
     2101 aaataaatga atgaagagca gtttaatata ccagatatta ttttgtatat aattttgggt
     2161 ttattaatat ttgcatcttt aagcggtata ttcagggagg ggctatatca ggacaatctt
     2221 tttgttcaat ctgcctggta tgggactgac ctaattacct ttatcttagc gatgccgata
     2281 tttgtaatat cgataatatt atataaaaag aagaccctct ttggcttatt attaatattc
     2341 gccatgttgt tttactcctt gtataactat gccttttatc tttttagtgc tgccctaaat
     2401 gatatgtact tagtatatgt ggctatcttt gctctctcat tttacagtat tttattattg
     2461 gtcagaaaaa taaatattga aaaggtagct gcgagttata aagagagtac acctacaaaa
     2521 ttgatcggcg gttatatggt tattgtagga atactattag gcgtatttca tattggctta
     2581 tctttaagtt atgtatttac cgaccaggta cctgctattg taacaaatgt tgcccatccg
     2641 accaatgtaa tagctgctct tgacttatca ctggtagtac cgattgcact aatcggcggt
     2701 atttctttat taaagaagca agcctggggt tatgtattgg ctgtggtctg gaactttaag
     2761 ggctttatat atatgactgc cctcacctca gcttcggtag ttgcatatat gaatggggca
     2821 accacgagtt tagccgagtt gtttctctgg gttccaattg cagttggctg tataatcatg
     2881 gcctatattt ctttgaaaaa tattgaaact atagattaaa taatatataa tcttgtgaaa
     2941 aaggacaaca caaggagtat tcaaaatatg aagagagttg aatttaaaaa taaaaggggt
     3001 ttaaccttaa tcggagattt atataaagct aaatcagacc atattgtgat catggcccac
     3061 ggttttatga atgacaggtc ttcccagggg cgttttgaac aagttgcgga gagattaaat
     3121 gaagcaagct ataatatatt aaagtttgat ttcagcggct gtggggaaag tgatgatgac
     3181 ataatagaac tggataagga agttgaggat cttttatcag cagtagaatt cgtgaaagaa
     3241 aaagggttta aaaatattgc ccttttcggt aatagcctgg gtggactagt ctgtctgaaa
     3301 gcatatagct cggatatctc taccatggtc ttaaccggtc ctattacgga cagtatgaac
     3361 tatgactggg aaaaatttta ttctaaagaa aagatggaag aattaaagga aaaaggttat
     3421 ttaacaatgg gtaatcaaaa aacagggatt agaaaaataa gtcaagatat gctggatgct
     3481 ttttctgaaa tcgaccagga agaaatatta aaaggtatag attgtccggt cctaatcatc
     3541 catggagacc ggggagaaga agaaaaagaa cttttagacc gctctaaaaa agctttaaag
     3601 atgctaccca atagttctaa attaaaagtg gttgaagatg cagcccatgg aataagagac
     3661 caataccaca tagtggtgga actaaccgta gattggatat taaacaactt tgagattgaa
     3721 taataattaa atattaatga ctaagactaa aggctgatat tgaatcagtc ttttttcatt
     3781 tttatcaata cttatgcctg attaaataaa atagattaac tgatactatg tttatagaat
     3841 aagaataaaa gaaaggttga taaaaatgaa aaagaaatta ttgggttatg ttggtaggtt
     3901 cactttgtta cacgtaatta cttacacagt cgcgggagtt attttcatga acgtttttaa
     3961 ctatgcggaa gtttttgaga cttcccaggt ctttgcccac tttcggtcgg ttgattcacc
     4021 gataatgcag gctgcccctt ttttgcagat tatcagaggt ggtttctttg ctttaatcct
     4081 ttacccattc tatgaggtaa ttgtaaacca taaaagaggc tggttagtat tatttggagt
     4141 tctctggggt ttaacctaca taggttctgt agaagcaact ttaggctcaa tcgaaggttt
     4201 aatttatact aaggcaactc tagtcgaaca ttttgtgggg acaccggagg taacaattca
     4261 gatgctggtt ttttcctggt tatttgttaa atgggaaaaa agaagtataa aaaaagagaa
     4321 aacaagacag aagtccaaaa acccctaacg gtcgaaaact aaaaaagtga aaaatcatga
     4381 taaagttgat ataatataga aaagttcttt gaattaggag agataatatg acaggtgaaa
     4441 aaatcaaact gttaatagca gatgaccatc cttttttccg ccagggaatc agcctctttt
     4501 tagatgatac gtctgaaata gagattaagg cagaagcaga taacggaaag attgctctgg
     4561 acactattaa aaaagacaat gattttgatg taatattaat ggatttacag atgccggaaa
     4621 tggatggtat tgaggctact gcccaaatta aaagtgatta tccctctatt aaagtgttga
     4681 tcttaaccag ctttaacagc tggagtaagg tatatgaagc tttaaaagcc ggagcagacg
     4741 gctatataat gaaagattct aaaccgcacg aattagttgc tgctatcaaa gcagtgaata
     4801 cgggcggagt atattatggg gccgaggtag ctgaacagct cttaaaaagg gttaaccaaa
     4861 ctagtagtga agcagaaaat gaagaattaa tagagccttt aactgatagg gagctggaag
     4921 ttttagccct attaggtaga gggctttcta atcaagagat agctgatcaa ttggtagtta
     4981 gtattaaaac agttaagacc catgtctcaa atattctggc taaactggaa gttgacagcc
     5041 ggacccaggc agctgtattt gcgatccgga aaggtttaat ctaggtggtg gtaacaatca
     5101 ataagaatga aattagttct ttagaaaaat ataaaaatat tcatgacaat aagcaggcca
     5161 gttattggca aaacttaaaa gtcagttctt tatttggatt attattcggt atagtattct
     5221 taattgtaac cttaataacc agggattata ccggttggga catctatatt atacctatat
     5281 tatgtgctgt tatctcaggt atggccggtc tattagctac ttttttggaa agatactttc
     5341 ttaatagagg attagagaat gctttgataa gaaagaccat catattctcg gttgtaatta
     5401 cattaactat tactctgacc attgttgtat tcactcaaaa tagtgttgca aacttttttg
     5461 agactttaag aaataatatg atctggggaa ttgtctttgg agttatcttt gcagttgtga
     5521 tagctgccct ggaatattat aactggaaag tagaacagaa gatggccatc ctggaattag
     5581 agaataaata cttagaagat ctagcagcta aggatacgat gttaaaggaa gccacaaaaa
     5641 atctattggt ttccaaagaa agaaataaga tggcgcggga gctgcatgat tcaatatcac
     5701 aggatatcca tggtctatcc tatggattaa gttcactcaa aaaccagatg aagaccttag
     5761 atattgaaga tgaaaaagtt tttaaaatat tgaaccatct ggaaaaaaca gttgagaata
     5821 catcagaaga gttaaaatat atgattgaag aactaaaacc agccgcctta gagaaaaaca
     5881 gtttaaaaga tgccttatta actcacttag acttatttga ggcgagacag gatatagaag
     5941 tgaaaaagga aattgatgat ataaaggaac taagcccgga acaagaatac tcggtatata
     6001 ggatagtcca ggaggctctg gctaatatac aaaaacatgc taaagcagat aaggtaatag
     6061 tatattttaa aaaactgcca gataacaatt ttggaaaagc aaaattgacc attgaagata
     6121 atggtcaggg atttgatagt gataagatca aaaaaggaaa tgggcttgag aatatggaat
     6181 taagaagtaa gcagattggt ggtaattttc aagtaaaatc taaaacagga gccggaacac
     6241 agatcaaagt agaatttaat tattaaaaga aggtgggtta ataatggatt ttttaaaata
     6301 cgtaaaaaat aataagataa ctcttttaga tggggcgatg ggcactgaac ttaaaaaaag
     6361 agatgtaatg ggtaacttta aaaataatat agaaaatcag tcgatagtta aagaaattca
     6421 tgaaagttat attaaggccg gtagtaatgc aataataaca aatacctttt ccatgaatag
     6481 tctatatctt gatagtaaag atgccgatgt tggggtggaa ctaattaata aaaaaggtgt
     6541 agagattgcc caagaagcag ccggtgacga tatttttgta ttaggagata ttagttccac
     6601 cggtaaatta ttaaaaccct atggtgaata tgaagaaaat gcatttatcg atgtctataa
     6661 acaaaaggcg gagatattag ctgaaaatgg tgtagatgcc ttcataatag aaactatggt
     6721 ggatttaaga gaagctatct gtgcactaaa agcctgtaaa caagtatctg aactacctgt
     6781 aattgtttcg atttcctttg atagggttga acagggcggt cgaactgtaa tgggtaatac
     6841 agcccgtgaa gtggtagaaa acttaagtaa agaaaaggca gatatcatcg gtactaactg
     6901 tggatcgtta agtcctaaac agatggctga aatagtaaag atgatgagaa ctgaaactga
     6961 tttaccgatc atggtagaac caaacgccgg ccaaccaaaa ttaaaagggg aagagacagt
     7021 ctatgatatg cagccgaatg actttacagc cggtatcctg gcctgtatag aggcgggagc
     7081 taatattgta ggaggttgtt gtggaactag tcccgcacat atagatgacc ttaataaaaa
     7141 gttatttgga tagtgttttt aatagtaaag ttctaataaa ctttaaaggg aactccataa
     7201 agagttccct taataaatat atgttactta attatcctta gtcactatag gtttaccatc
     7261 agcatctact aatacagtga gtccaccacc acgaacataa aagtagttta caccggtttc
     7321 tttatctacg ataacttgat atgtatttgg tgtaattcct tgtgaagttg caagttcaaa
     7381 tcttttgtct ttacccataa tttataaaca ccaccttatt tattttaaaa aaataacaat
     7441 atctaccatt ttatattata ttatattaat atgcaagaaa ttttcttaga tttaatatat
     7501 tataattgga aattggaaaa tagatttatc aatagctact taattttcaa aaaaatgaat
     7561 ttttaggtat aaattcctaa attacttgac atatttatat tcattatgta ttattataag
     7621 taatcaaaat aaaatatag
//
LOCUS       DATGCJ010000101         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_191416, whole genome shotgun sequence.
ACCESSION   DATGCJ010000101 DATGCJ010000000
VERSION     DATGCJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191416"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1421)
                     /gene="ltrA"
                     /locus_tag="VKN64_05100"
     CDS             complement(120..1421)
                     /gene="ltrA"
                     /locus_tag="VKN64_05100"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HMA59653.1"
                     /translation="MVRDKWYTLYGHLLSFNRLKKAFRKVKKNGGAPGTDGQTIAQFD
                     DDIIENLEEIKEELQEKSYHPSPVKRVYIDKGNGEKRPLGIPTVKDRVVQQVLKDVIE
                     PIFEPIFLECSYGFRPEMSAHMAIKQVKEYLKEGYVWVVDADIKSYFDTINHKLLIDA
                     VAEKISDGSILNLIGMFLESEIKDGEGLTIPEMGTPQGGVISPLLANIYLHKFDKEMT
                     ERGHKLVRYADDWIILKRTMLAGRRIMSGAKAYLQEELKLKMHPKKSKLIDARKESFE
                     FLGYYFRIYEDKGQGKDNVIFGPSYKSIDKFKDKIREITRRNQTTSAKAIIDRLKPKL
                     RGWGNYFGIGWVKSLFRKLDSWIKRRIRMIQLRSWKNPGKLVAILKNKGWKGKIKRIS
                     MTSWKNSSCQIVHFIMDNEWFQDNGWVSLTDVRQKIMSQNG"
     gene            complement(2149..3030)
                     /locus_tag="VKN64_05105"
     CDS             complement(2149..3030)
                     /locus_tag="VKN64_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59654.1"
                     /translation="MKRNWLVVLVVAFVLMFSVGVLAQENPAGTGDGIWTSSEYWEEY
                     DYGTEYNRTGDDGIIWSNLVDPQNPNSDFVNLVGLDRSGYTTDVNRARGDNELDNDQK
                     LEINLPVFAYIPCYLEITLTGNEGKSSLQSFGPDATATRSTLSGVTGPPPGYLGYQLL
                     FDNEIGGFIDADWKSLGAGKNAEIEPGSDIYIAGCDIFVVEVYGNEAFTYEVQSSPLM
                     GMGGATLNMDIRTSMNLGDSWPYEATFDSVDEVAFDKDYDAGNGGTFLHNFRVPYTRN
                     SVHGSYEGDVIFRVVSR"
     gene            complement(3095..3925)
                     /locus_tag="VKN64_05110"
     CDS             complement(3095..3925)
                     /locus_tag="VKN64_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59655.1"
                     /translation="MRKIYILLTIVFLILLSFPALSIENIYLGSFSPEKELAIEFTVN
                     FKNDDSFENKIIIEFKDAEFISQNSIIPNSRITSNFRTGIISFQKDQTIISMNQLKKE
                     TDYYILPLNLKLFPQDKPGLFENTILIKNTNGDLLYEKNIIFEVNPWTIFELSKEKYL
                     RIMNSNNNQKLKSNGEVIIKVASNSSWELYGLLNDEAKEFADKISLFIKQDDNYRIVK
                     KGPFQIHSKSQLITQGTKTVSEENYWEEIEILMEIDEIKNIKSGLKIFPVIFYLYSIN
                     "
     gene            complement(4032..4358)
                     /locus_tag="VKN64_05115"
     CDS             complement(4032..4358)
                     /locus_tag="VKN64_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59656.1"
                     /translation="MEENMGAFVCDKNVFNRILRLEKRRGDKYLFLVITFDTYIENNK
                     FKKIFDNLISLLRSKDVITKNKDQILILLNNANLLYGEDIVNRFNIMFEKNHPEIKPV
                     FTLKNF"
BASE COUNT         1379 a          943 c          518 g         1663 t
ORIGIN      
        1 gacttacacc tattatcaga ataatttacg aagcacacaa ataaatcgag taggggagat
       61 ttccactccc ctctcacaga accgtacgta cgggccaacg tatacggctc ttccaaagat
      121 tatccgtttt gggacattat cttttgacga acatcagtaa gacttaccca accgttatct
      181 tgaaaccact cattatccat tataaaatga actatctgac aactggagtt tttccagctt
      241 gtcatagata tcctctttat cttccccttc cagcctttgt tctttaaaat cgctacaagc
      301 tttcccgggt ttttccagct tctcagctga atcattcgga ttcttctttt tatccaacta
      361 tccagctttc taaataatga tttaacccat ccaatgccaa agtagttacc ccaacctctc
      421 aatttaggct tcagcctatc tatgattgct ttagcactgg tagtttggtt tctccttgta
      481 atttccctga tcttgtcctt gaatttatct attgatttgt acgaagggcc aaaaataaca
      541 ttatcttttc cttgaccctt gtcttcatat attctaaaat agtatccaag gaactcaaag
      601 gactccttac gggcatcaat tagtttactc tttttagggt gcattttaag cttcagttct
      661 tcttgaagat aagctttagc tccagacatt atacgtcggc ctgccagcat tgttcttttt
      721 aatattatcc agtcatctgc atatctcact agtttatggc ctcgttcagt catttcctta
      781 tcaaacttat gaagatatat atttgcaagt aatggactaa ttacgccgcc ctgaggagtt
      841 cccatttctg gaattgttaa tccttcccca tctttaatct cactttctaa aaacatcccg
      901 attaaattta atatcgaacc atcacttatc ttttcagcaa ccgcatctat taatagttta
      961 tggttgattg tatcaaagta tgatttgata tcggcatcta ctacccatac ataaccttct
     1021 tttaaatatt ccttcacctg tttgatggcc atatgtgcac tcatttctgg tctaaatcca
     1081 taagaacatt ctaagaaaat cggctcaaat atcggttcta tcacatcctt tagtacctgt
     1141 tgaactaccc tatctttaac tgtgggtatt cccagcggcc gtttctcccc attaccttta
     1201 tctatataaa ctctcttaac tggggaagga tgatagcttt tctcctggag ttcttctttg
     1261 atttcttcta gattttctat gatatcatca tcaaattgtg ctatggtttg accgtctgta
     1321 cctggagcac ctccattctt tttgacctta cgaaatgctt tctttaatct gttgaatgaa
     1381 agtaaatgtc cgtatagagt ataccacttg tctcttacca tgttttgcct ccaattctga
     1441 aacacatttc catatctcct taagctgtag actccaattt tcattttgca taaatgtcca
     1501 tcgttagtcc ttaacgcctc attggctagg cacaattcat accatttaac cctcaaaact
     1561 actgaattga ggtcaatgca actctacctc ttaccctaca actcaaagct atatttccat
     1621 gtcttttaat cttggttcct gccttcgcct ctgctgattt aatcaccagc aggatactct
     1681 tttaaagtat ttattcggat tttatcctcc acaccgttgc cggctttcat aggtccgaat
     1741 ttcatcgcta ctatgcattc atctgaactc tcatattaat ttggaaaacc ttcggggtct
     1801 ccttatggtt caccatttcc tctaggataa tatgagcact acctctctgg atcatttaga
     1861 taacttccct tctaacacaa ccatcctaac atttatcagg tttccaacta aggacttcac
     1921 catattttgc aagttcatcc cctgatacct gccaacactg gttcgcttac gctttgttca
     1981 gaagtttgcc tgagaacctt tcgaaccctg tttcacaaca ccgctctgtc catcagctaa
     2041 ctatttcgtt ggattcatag tacaggactc taacctgcta gttatctaaa ctgccagaca
     2101 tacaaaaagg gctgatatta agatcagccc ttttaaatgt aaatatactt acctacttac
     2161 cactctaaat attacgtcac cttcataact accatgaaca ctatttcttg tataaggtac
     2221 tctaaagttg tgtaggaatg ttccaccatt accagcatca tagtctttat caaatgctac
     2281 ttcatctaca ctatcaaatg tagcttcata tggccaagaa tccccaagat tcatagaagt
     2341 tcttatatcc atatttaaag tagctccacc cattcccatt aatggtgaag attgaacttc
     2401 ataagtaaaa gcctcattac cataaacttc tactacaaaa atatcacaac cggctatata
     2461 tatatctgaa ccaggttcta tttctgcatt tttacctgct cctaaagact tccaatctgc
     2521 atctataaat ccgcctattt cattatcaaa cagtaattga taacccaaat aaccaggtgg
     2581 aggaccagta actccactaa gtgtacttct tgtagctgta gcatctggac caaagctttg
     2641 taaactagat ttgccctcat tacctgttaa agtaatttct aaataacaag gaatataagc
     2701 aaaaaccgga agattaattt caagtttttg atcattatct aattcattat ctcctctagc
     2761 tcgattaaca tcagttgtgt aaccagacct atctaaacct actagattta caaaatctga
     2821 atttggattt tgtggatcta ctaaattcga ccaaattata ccatcatcac cggttctatt
     2881 atattcagtt ccataatcat attcttccca atactcacta gatgtccaaa taccgtctcc
     2941 agttcctgca gggttttctt gagctaatac accaacagaa aacatcaata caaaagctac
     3001 caccaacact actaaccaat ttcttttcac ttctaactcc cccttattat tattgtttat
     3061 aatgattgac tgtttttata cagtctatta tcacctaatt tattgaatat aaataaaata
     3121 tcactggaaa aattttaaga cctgatttaa tattttttat ttcatctatt tccataagaa
     3181 tttctatttc ttcccaataa ttttcttctg aaactgtttt agtcccttgt gtaattagct
     3241 gagatttgct atgtatctga aaaggacctt tttttactat tctataattg tcatcttgtt
     3301 ttataaaaag acttatttta tccgcaaatt cttttgcttc atcatttaat aaaccataca
     3361 actcccaact agaattggaa gccactttta ttattacttc tccattactt tttaactttt
     3421 gattattatt actattcata attcttaaat atttttcctt ggataattca aatattgtcc
     3481 agggatttac ttcaaaaatt atattctttt catataatag atctccattt gtatttttaa
     3541 ttaatattgt gttttcaaac aaacctggtt tatcttgtgg aaacaatttt aagtttagag
     3601 gtaaaatata ataatcagtt tcttttttta attgattcat agatattata gtttgatcct
     3661 tttgaaaact tattatacca gtcctaaaat ttgatgttat acgagaattg ggtattatag
     3721 aattttgact tataaattca gcatctttaa attctataat aattttattc tcaaaactat
     3781 catcattttt aaagtttaca gtaaactcta tagctaattc tttttcagga gaaaaactac
     3841 ctaaataaat gttttcaata cttaaagctg gaaatgataa taatataaga aatactatgg
     3901 ttaaaagtat ataaattttt ctcactttcg cccctcctca tatacaatct ttttaatcca
     3961 atattataat aacatttgaa tatatatatt atttttaaaa attctcgaaa atacatctaa
     4021 aaaatatctt tttaaaaatt ttttaaagta aaaacaggtt ttatctcagg atgatttttc
     4081 tcaaacataa tattaaatct atttacaata tcttctccat aaagaagatt agcattattc
     4141 aatagaataa gaatctgatc tttgtttttg gtaattacat cttttgatct taataatgaa
     4201 atcaaattat caaatatttt tttaaactta ttgttttcaa tatatgtatc aaaagtaata
     4261 accaagaaca aatatttgtc tcctcttctt ttttctaatc ttaatattct attgaaaaca
     4321 tttttatcac aaacaaatgc acccatattt tcttccacac taatcacctg cttttaaatt
     4381 tatttacatt ctatacataa tataacatct tctattagat ttaaactcat tattatttat
     4441 aaaactctgc taaaattatt ctttatctaa cttttaccta taaaataaaa aaactccgca
     4501 aat
//
LOCUS       DATGCJ010000102         6791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_592714, whole genome shotgun sequence.
ACCESSION   DATGCJ010000102 DATGCJ010000000
VERSION     DATGCJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592714"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VKN64_05120"
     CDS             complement(<1..744)
                     /locus_tag="VKN64_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HMA59657.1"
                     /translation="MKAEENNHHEKMVQDFKKRFYISLVVTIPILILSPMIQSFLNIE
                     FKFTGSNYILFILSTFIFFYGGWPFLKGLYDELSDKQPGMMTLIALAISVAYIYSSAV
                     VFGLAGRFFFWELATLIVIMLLGHWIEMRSVMSASRALEELARLMPDTAHKIEGDEVK
                     DVPIKELNVGDKILIRPGEKIPADGEIDDGSSYINESMLTGESEPVEKSEGEEVIGGS
                     INGDSSLEVSIKKAGDDSYLSQVINLVEEA"
     gene            complement(858..1424)
                     /locus_tag="VKN64_05125"
                     /pseudo
     CDS             complement(858..1424)
                     /locus_tag="VKN64_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            complement(1437..1940)
                     /gene="msrB"
                     /locus_tag="VKN64_05130"
     CDS             complement(1437..1940)
                     /gene="msrB"
                     /locus_tag="VKN64_05130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMA59658.1"
                     /translation="MLNNNYIVLISLCLLLLMILSPTVSAIKVDGEYPIELFDDFWQE
                     KLSGGQYNILKNSGTEPAFNNKYYRNKEEGLYVCAACGNFLFSSDHKYESGSGWPSFY
                     DVYSDQSVVTRPDNSLGMQRTEVICARCGGHLGHLFEDGPEPTGLRYCINSAAMKFYQ
                     YAYFAHG"
     gene            complement(1945..2247)
                     /locus_tag="VKN64_05135"
     CDS             complement(1945..2247)
                     /locus_tag="VKN64_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HMA59659.1"
                     /translation="MNEDEKFKKAKERVKEIKDFYGHLLVYVVVNIGIFLINYIVSPG
                     TYWFYWVLIGWGIGLAVHWIQVFGIGKFLDKKWEEKKVKEIMKEMEDSEESQKEKE"
     gene            2411..3649
                     /locus_tag="VKN64_05140"
     CDS             2411..3649
                     /locus_tag="VKN64_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HMA59660.1"
                     /translation="MKIREFQFDKDFERIFNIWREVGWINDEERKREALKYFVKAGKG
                     KVTELNNEVEGYVNSTPGYYQYLDTQFDLHALSAVTISRVARKQGIGTKATAEAIAES
                     AASGAHISALGMFEQGFYDKLGYGTGSYEHLVSFDPAHLDIPGKAPIPARIKVEDYKK
                     VHQAKLERTKRHGYCTLLPPEVTRAEMFFGDKEPFGLGYKDDKGNITHHIWVIPSEEL
                     HGPYFIRWMTFNNLEQFFDLMNLIKNLGDQIHLVKIWEPAGIQFQNLIKKPIKDFHVT
                     KNSDYQVINKAVAYWQIRILDLKSCINNTSLQGEPVEFNLDLKDPIEKYLDKSSPWQG
                     LSGQYTLKISKNSAIKEGTNVDLPTLKTDINTFTRMWIGAVRPSLLPYTNSFEAPAEL
                     IDKLDRLFITLPTPKIDWDF"
     gene            3741..4055
                     /locus_tag="VKN64_05145"
     CDS             3741..4055
                     /locus_tag="VKN64_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59661.1"
                     /translation="MLTHFLESNENIKYVNGPTLAEIPTGRRTINLNNINKENIGFLN
                     EYYQGMDQLSSLYKKEDKFYASFSLFPTNDFNGNDLNQIENYLKENNIKYFVSGDLYI
                     QQ"
     gene            4088..5644
                     /locus_tag="VKN64_05150"
     CDS             4088..5644
                     /locus_tag="VKN64_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMA59662.1"
                     /translation="MPPTALILILFVFKTQMGNLKATFFSILPAGIAALWTLGIVGWS
                     GLEVSIITVLAPIFTIVIGSADGLHFVSHLQESLKEGKDKITSIIKTLKMVGIPMIIT
                     TVTSMVGFLSLLVMNTQAIYDLALFASLGIFLAGIATWFVLPLILSGNIDVPYKVKSK
                     KLFTSIIKRLWGKASIAILILLLLIAAFGIQHIKTEFNMPMIYRDFTSVHKSFEKIKE
                     ITGGSLPLYLLINTEENPLEEKYAQSILKIQKDLENKNLAAKTMSVYDIYSLIYSNFI
                     NTEKREYPNMTRIKMINRMAFSEGGYNPTQQFINHAENYTRVFIFPKNLQNKTLEDIS
                     NFIGNKKEEFEGMNMSLTGVQYLMHDLNQNMLNNQKNTLILAFILVFFLLLISLRKII
                     PSIISILPIGFTVIILFGFLGISNISLNLFTATIFSITIGVGIDYAVHFTSVWMSFKK
                     KGYNTQEAVDKAYDYTSRPIMANAFGLSLGLSALLLSPLRIHLYVSFLMWVSMISGVF
                     FKPVLLAHYS"
     gene            5738..6631
                     /locus_tag="VKN64_05155"
     CDS             5738..6631
                     /locus_tag="VKN64_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMA59663.1"
                     /translation="MNIELKKVELTNGETLGYRKRAGGNKLLLLLHGNMTSSKHWDLF
                     LENFNEEYTLIAPDMRGFGISTYNNTIDSIEDFVSDLELFVDELNLKEFSLMGWSTGG
                     CTAMQYTANNQEKVNKLILLESISSRGNPLFELDEEGNIIPEKRIATMEAVANDEHNI
                     KPTLNAIENENKAFMKSVWNTLIYNNNKPSEERYDEYLEDMFTQRNLVEVYHALNHFN
                     ISDKHNGLAEGTGEAAQITIPTLVLYGENDNIIPANMPDEIVEDIGENAELEILKNSG
                     HSPLIDDLEQLIEKVNQFLKK"
BASE COUNT         2294 a         1190 c         1076 g         2231 t
ORIGIN      
        1 agcttcttct actaaattaa tcacctgaga taaataactg tcatcaccgg cttttttaat
       61 tgaaacttcc agagaactat ctccattgat tgaaccaccg atgacttctt cgccttcact
      121 tttttcaacc ggctctgatt ccccggttaa cattgattca ttaatataac tggaaccatc
      181 gtctatttct ccatcagccg gtatcttttc tcccggccta attaatattt tatctcctac
      241 atttaattct tttatcggga catctttaac ctcatcgccc tcaattttat gagccgtgtc
      301 cggcattaaa cgggctaact cttctaaagc ccgtgaggca ctcatcactg aacgcatctc
      361 tatccagtga cctaagagca taatcactat taaggtagcc agttcccaga aaaagaacct
      421 ccctgctagt ccaaatacta ccgcactgct gtatatatat gctaccgaaa tcgccaaggc
      481 aattaaggtc atcatacccg gttgtttatc actaagttcg tcatatagac cttttaaaaa
      541 gggccaacct ccataaaaga atatgaaggt tgaaaggata aataatatat agttactacc
      601 ggtaaactta aactcgatat ttaaaaatga ttggatcatg ggagaaagaa tcaaaattgg
      661 tattgtaact actaaagaaa tatagaacct ctttttgaag tcctgtacca tcttttcatg
      721 atgattgttc tcttcagctt tcattacttt ttaacctcca atatcaatcc tgtttttaac
      781 ttatcaattt cgagtctttt tatcaagttt atgttttaca aattttctaa ttggaagtta
      841 tattaaaatt attattttta attattaatt gcgtctttga tagtttgaat ttgctcggga
      901 tagattggag atagatatga gttgtccaat atttctacca tctcttccga agtgataaat
      961 ccagatcgat aagcgtttag tttagcagct atatgagaat ctattaaagc ttcttcatta
     1021 tcaaataggg aaagtgtttt ttgatagagt tgattactgg ttctcagttg gtatttttga
     1081 tggtagtttt ctgctatata gaatttttct aattcttctg gactgaaaaa gagattatcg
     1141 gcttctcttc ttttttgagc caattcttta atatactctt cttgtttctc atttgcataa
     1201 aatacaatat atctatactg tcctgtggtt ggtctaacat ttgaactggt aatttcaata
     1261 taatgatcaa ctaaatcctg ataacttatt ttttgtgggt caaatattac cttggctgtt
     1321 tcagcatggt ctcctatgtt attataagta ggattatcag tagttccacc tgcataacca
     1381 acatgagtgt gtacaacacc gtctaaggct ccaaactgag cctctacacc ccagaatcag
     1441 ccgtgggcaa aataggcgta ttgataaaac ttcattgcag cggagttaat acagtatcta
     1501 agcccagttg gttcgggtcc atcttcaaat aggtgaccta aatgaccacc acagcgggca
     1561 cagataacct ctgttctctg catacctaag ctattatcag gtcttgtaac cactgactgg
     1621 tctgagtaaa catcataaaa gctgggccaa ccactgcctg attcatactt atggtctgaa
     1681 ctaaaaagaa aattaccgca ggctgcacaa acatataagc cctcttcttt attcctataa
     1741 tatttgttat taaaagccgg ttcagttcca ctatttttta atatattata ttggccgcca
     1801 gacaatttct cctgccaaaa atcatcaaat aattctatcg ggtactcacc atcaacttta
     1861 attgccgaaa cggtcggact taagatcatt aacaacaata aacacaagga tattaaaaca
     1921 atataattat tgttcagcat ttttttactc cttttctttt tgggattctt ccgaatcttc
     1981 catttctttc ataatttctt taaccttttt ttcttcccac tttttgtcta aaaatttacc
     2041 aatgccaaat acttgaatcc agtgaactgc cagtccaatt ccccaaccaa tcaaaaccca
     2101 ataaaaccag tatgttcccg gtgatactat ataattgatg agaaatattc caatattaac
     2161 aaccacatat accaataaat ggccgtaaaa atcttttatt tctttaactc tttccttcgc
     2221 ttttttgaac ttttcatctt cattcatgac aaccgcctcc tccttttatt cctatagtaa
     2281 tagtttcttt tattattaat aaaatccttt attttttaat attattaaat atttgtaaaa
     2341 aaaaggtgtt ttttattatc ttaaagaata ttaatattac agatatatta ttatgtaaga
     2401 ggtgatgttc atgaagataa gagaatttca atttgataaa gattttgaaa ggatttttaa
     2461 tatctggcgg gaagtcggct ggataaacga tgaagaaaga aaaagagagg ccttaaaata
     2521 ctttgtcaaa gcaggcaagg gaaaggtaac tgaattaaat aatgaagtag aaggatatgt
     2581 taactctacc cccggttatt atcaatatct tgatacacag ttcgatctac acgctttaag
     2641 tgctgtaacc ataagcagag tggctagaaa gcagggcatc ggaaccaaag ctacggcgga
     2701 agctattgca gaatcagctg cctctggtgc ccatatctca gctttaggta tgtttgaaca
     2761 gggtttttat gacaaattgg gttatgggac aggtagttat gaacaccttg ttagttttga
     2821 ccccgctcat ttagatatac ccggtaaagc ccctattcct gcccgaatca aagtagaaga
     2881 ttacaaaaag gtacatcaag ctaaattaga acgaaccaaa agacatggct actgtacact
     2941 tttgccacct gaagtaacaa gagcagaaat gttttttgga gataaagaac catttggctt
     3001 aggatataaa gatgataaag gtaatattac acaccatatt tgggtaattc ctagtgaaga
     3061 actacacggc ccttatttta ttagatggat gacttttaat aacttagaac agttctttga
     3121 tttgatgaat ttaattaaaa atctaggtga ccagatccat ttagttaaaa tctgggaacc
     3181 agctggaata caatttcaaa atctaataaa aaaaccgata aaagacttcc atgtaactaa
     3241 aaatagtgat tatcaggtaa ttaataaagc agtagcatat tggcaaatta gaatattaga
     3301 tctaaaaagt tgtatcaaca atacttcttt acagggagaa cctgttgaat tcaatttaga
     3361 tttaaaagat cctattgaaa aatatctgga taaaagctca ccctggcagg gtttatccgg
     3421 ccaatatact ttaaaaataa gtaaaaattc agctattaag gaaggtacta atgtagatct
     3481 gccaacactg aagacagata taaatacctt tacaagaatg tggattgggg cagttagacc
     3541 gagcctttta ccatatacaa actcctttga agcaccggca gaattaattg ataaattaga
     3601 tcgtttattt ataactcttc caacccccaa aattgattgg gatttttaaa ttaaataaga
     3661 aatactaatt ttagagttct gtttaacagg actctttttt tatttgacaa aataattttc
     3721 ttgtttttaa aatatagtaa gtgcttactc actttttaga gtcaaatgaa aatattaaat
     3781 atgttaatgg acctaccctc gctgaaatac caaccggtag acgaacaata aatttaaata
     3841 atattaataa agaaaatatc ggttttctta atgaatatta tcaaggaatg gatcagctat
     3901 caagtctata taagaaggaa gataaattct atgcctcttt ttcgcttttt cctacgaatg
     3961 attttaatgg aaatgattta aaccaaatcg aaaattattt aaaagaaaat aatataaagt
     4021 acttcgtttc cggcgatctc tatattcagc aataatcatc gattatatct tattaatttt
     4081 actttttatt ccaccaacag ccctcatcct tatcctgttt gtctttaaaa cccagatggg
     4141 caacttgaaa gcaaccttct tttctatcct gccggctgga attgccgccc tctggacctt
     4201 aggaatagtc ggctggtcgg gcttagaggt ttccataata actgtattgg caccaatatt
     4261 tacaattgtt attggtagtg cggatggact gcattttgtt tctcatctcc aggaatcttt
     4321 aaaagaaggt aaagataaaa ttacaagtat cataaaaaca ttaaaaatgg ttggtatccc
     4381 gatgataata actacagtta cttctatggt cgggttttta tcacttttgg tcatgaatac
     4441 ccaggccatt tatgaccttg ccttatttgc ttcattaggc atatttttag ctggaattgc
     4501 cacctggttc gtactccctt taatcctatc tggaaatatt gatgtccctt ataaggttaa
     4561 aagtaaaaaa ttattcacct caattataaa aagactctgg ggtaaagcta gtatagcaat
     4621 tttaattttg ctgctcttaa ttgctgcctt tggaatacaa cacatcaaaa ctgaattcaa
     4681 tatgcccatg atttatagag atttcacctc tgttcataaa agctttgaaa aaatcaaaga
     4741 aataacagga ggcagtttac cactttatct tttaatcaat accgaagaaa acccactgga
     4801 agaaaaatat gcccaatcaa ttctaaaaat ccaaaaagat ctagaaaata aaaatttagc
     4861 agctaaaaca atgagtgttt atgatattta ttccctgata tacagcaact tcattaatac
     4921 tgaaaaaaga gaatatccca atatgacgag aattaagatg ataaaccgga tggctttttc
     4981 agaaggtgga tataatccaa ctcaacagtt cataaaccac gctgaaaatt ataccagggt
     5041 gtttatcttc cctaaaaact tacagaataa aacattagaa gatatttcaa acttcatagg
     5101 aaataaaaaa gaagaatttg agggaatgaa tatgagctta accggggtcc aatacttaat
     5161 gcatgatctt aatcaaaata tgttgaataa tcaaaaaaat acattaattc tggcctttat
     5221 tttagtattt ttcctccttc taatttcact tagaaaaata attccctcta ttatatcaat
     5281 attaccgatt ggttttacag tcattatcct tttcggattt ctaggtataa gtaatatatc
     5341 attaaatcta ttcacagcaa ctatttttag tatcacgatt ggagttggta ttgattatgc
     5401 tgttcatttt acttcggtct ggatgagttt taaaaaaaag ggctataaca ctcaagaggc
     5461 agtggataag gcttatgact atacctcaag gccgatcatg gccaatgcct ttggcttatc
     5521 attaggttta agtgctttat tattgtcacc tttaagaatc catctttatg tctcattttt
     5581 aatgtgggtt tcaatgatct caggcgtttt ttttaagcct gtccttcttg cccactattc
     5641 ttaaaaaaat taaataagca ggttaaacca aaattttatc taaattatat attagataat
     5701 actttaggaa ggtataatta agggagggaa actttaaatg aatattgagt taaagaaagt
     5761 tgaactaacg aacggagaga ctttaggtta taggaaaaga gccggaggaa ataaattgct
     5821 tctcttatta cacggaaata tgacctcatc taaacactgg gacctctttt tagaaaattt
     5881 taatgaagaa tataccttaa ttgctcccga catgcggggt tttgggatct caacctataa
     5941 taataccatt gattcaatag aagactttgt aagtgattta gaattatttg ttgatgaact
     6001 taatttaaaa gaattcagtt tgatgggttg gtcgaccggg ggctgtacag ccatgcaata
     6061 tactgccaat aatcaggaaa aagttaataa attgattctc ttagaatcga tcagttcacg
     6121 cggcaatcca ttgtttgagc tcgatgaaga aggtaatata atacccgaaa aacggatagc
     6181 cacaatggaa gcagttgcta atgatgaaca caatattaag cctactctca atgcaattga
     6241 aaacgagaat aaagccttta tgaaaagtgt ctggaacact ttaatctata ataataacaa
     6301 accgtccgaa gaaagatatg acgaatatct tgaagatatg tttacacaaa gaaatttagt
     6361 ggaagtctac catgctttaa accactttaa tatcagtgat aaacacaatg gacttgcaga
     6421 aggaactgga gaggccgccc aaataacaat tccaactttg gtcctttacg gggaaaacga
     6481 taacataata ccagccaata tgccggatga aatagtagaa gatattggtg aaaatgctga
     6541 gttagaaata ttaaaaaatt ctgggcactc cccccttatc gatgacctgg aacaattaat
     6601 agaaaaggta aatcaattcc tgaaaaagta agtttaatct ataaatagtt aatctcccat
     6661 catctatggg agattagctt ttttattcta ataatttatt ttttagctat tataaaaaat
     6721 ctatgctctg aaacttcaaa ataattattg gactgaataa actcgtgtat ttcttttaat
     6781 ttaggtttaa a
//
LOCUS       DATGCJ010000103         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_12691, whole genome shotgun sequence.
ACCESSION   DATGCJ010000103 DATGCJ010000000
VERSION     DATGCJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12691"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VKN64_05160"
     CDS             <1..205
                     /locus_tag="VKN64_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59664.1"
                     /translation="PFGDKTIDVIKKTDDYLKQLAQNWDGKIIITSDHGMHSTKEGRD
                     HGSVLYQDMFVPYLITEGGNSNE"
     gene            198..713
                     /locus_tag="VKN64_05165"
     CDS             198..713
                     /locus_tag="VKN64_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMA59665.1"
                     /translation="MNKKMIIIVVILMAATVGVTAFLNRGSIAEKENKQAEALVTFKE
                     AGNEIETVNMSYVKKQGEKTFSKELDTSDSGPVEHTYTGVPLKNVIKEVGINLEDKSQ
                     VIVKAVDGYTVALTAKEVREDDNVYLVYKSDGNLLKSKKEGGTGPYRLVIRNDQFGQR
                     WCKFVTEVNVK"
     gene            710..1399
                     /locus_tag="VKN64_05170"
     CDS             710..1399
                     /locus_tag="VKN64_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59666.1"
                     /translation="MSQPIRAKNLSFSYSKDGKKIIDNINLQVNTGEILAVVGLSGSG
                     KTTLCYCLSGIIPHLYNGELSGEVYINDQLIKEMNMPQIATKVGVVFQNPETQIFFPM
                     VENELAFGPENLCIKREQIGQRIEEVLSLLDMEDKRYDKTEHLSGGQKQLIALASVLT
                     LKPEILIFDEVMAQIDQKGKQAIKDIILQLKKEGKTVIMVEHNLDNIDIADRKLVLKN
                     GRLKKFEGSLQ"
     gene            1380..2057
                     /locus_tag="VKN64_05175"
     CDS             1380..2057
                     /locus_tag="VKN64_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59667.1"
                     /translation="MKEVCSSNPFIEVNKVTFSYKKSDIILNNITTSFDKGEFAALIG
                     PNGSGKTTFGKLMTGILSANEGSVFVEGQNVEEISLGNIGKKIGYLFQQPARQLFNPT
                     VKEEIGFAHSFMGKPKEVVEQKVNEIIEEFSLQGLEDASPYRISRGERQRVALASIFI
                     NEPSFLILDEPTTGLDIVRKEQLAKLLKNLQSRGIGIIVISHDKDFINKNADRKIELY
                     GGELHEN"
     gene            2047..2775
                     /locus_tag="VKN64_05180"
     CDS             2047..2775
                     /locus_tag="VKN64_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HMA59668.1"
                     /translation="MKIDPRTKLVMVFSLSTLAVFVVDILLLSIVLIISIIVSNFFKE
                     DWGFLNRACKFIVLLVGIAFLQSIFSPSGEILFAIKDFTLLTTGGLAKGIRVILRMLV
                     IIISATIMKGSSPREIIQGLIQWKVPYEIAFMVSLAIRFIPILGQEAKDAFTAIQLRG
                     IEIDKLSLTKRFKVYSYMFMPVLSGVIHRAKELSTSLEARAFRAYPERTSYIKLELSK
                     NDLLIMSASIVFTITMLVIYILLR"
     gene            2781..>3135
                     /gene="mobB"
                     /locus_tag="VKN64_05185"
     CDS             2781..>3135
                     /gene="mobB"
                     /locus_tag="VKN64_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HMA59669.1"
                     /translation="MKLFSVFGISLTGKTTTIENIIKELKKRRYSIGSVKEIHYEDFA
                     IDKEGTNTYRHSQAGAELVTARGYNETDILYKERLSLQKILRNYSQDYVILEGVEKGN
                     FPKIITAKESEEIEER"
BASE COUNT         1128 a          438 c          647 g          922 t
ORIGIN      
        1 accattcgga gataaaacaa ttgacgtaat taaaaagacc gatgattatt taaaacagct
       61 ggcacaaaat tgggatggta agataataat aacatctgac catggtatgc attctactaa
      121 agaaggtagg gaccatggtt cagtccttta tcaggatatg ttcgtaccct atctgattac
      181 ggaaggaggc aacagtaatg aataaaaaga tgatcataat tgtagttatc ctaatggcag
      241 ctacggtagg tgttactgcc tttctaaata ggggtagtat tgcagaaaag gaaaacaagc
      301 aagcagaagc tttagttact ttcaaagaag cgggcaatga aatagaaacg gttaatatga
      361 gctatgtgaa gaaacagggt gagaaaacat ttagtaaaga gttagatacc agtgattcag
      421 gcccggtaga acatacttat acaggtgtac ccctcaaaaa tgtaatcaaa gaagtgggta
      481 tcaacttaga agataaaagc caggtaattg ttaaggctgt agatggttat acagttgctt
      541 taactgcaaa agaagttagg gaagatgata atgtttattt agtatataaa agtgatggta
      601 atcttctgaa aagtaaaaaa gaaggaggca ccggccctta tagattggtt attagaaatg
      661 atcaatttgg acaaagatgg tgcaagtttg taacggaggt aaatgtgaag tgagccaacc
      721 cataagagct aaaaatctat ctttcagtta ttcaaaagac ggtaaaaaga taattgataa
      781 tataaattta caagtaaata cgggagaaat attagctgta gttggtttaa gcgggagtgg
      841 taaaacgacc ttgtgttact gcctcagcgg tataatacct catctatata acggggaatt
      901 atcgggagaa gtatatataa atgatcaact gatcaaagaa atgaatatgc cccaaatagc
      961 gactaaggta ggtgttgtat ttcaaaatcc ggaaacccag atattttttc cgatggtgga
     1021 aaatgaactt gcttttggtc cggaaaacct ttgtattaaa agagagcaaa tcggtcaacg
     1081 gatagaagag gtactttcct tactagatat ggaagataag aggtatgata aaaccgaaca
     1141 tctttccggg ggacaaaagc agttaatagc tttggcttct gttttaacct tgaaacctga
     1201 aatcttgatc tttgatgaag ttatggccca aattgatcaa aagggaaaac aagctattaa
     1261 ggatattata ctacagttaa aaaaagaagg taaaactgta attatggtag aacataattt
     1321 agataatatt gatatcgcag accggaaact tgttttaaag aacgggaggt taaaaaagtt
     1381 tgaaggaagt ttgcagtagt aacccattta tagaagtgaa taaggtaacc ttttcttata
     1441 aaaaatccga tattatatta aataatatta caacctcctt tgataaaggt gaatttgctg
     1501 cccttatagg acccaacggg agtggtaaaa ccacctttgg taaattaatg acaggaattt
     1561 tgtctgcaaa tgaggggtca gtttttgtag aagggcaaaa tgtagaagaa atctccctgg
     1621 gaaatatcgg taaaaaaatt ggataccttt ttcaacaacc agcccgccag ttatttaacc
     1681 ctaccgtaaa agaagaaatt ggcttcgccc attcttttat gggaaagccg aaagaagtag
     1741 tagaacaaaa ggtaaatgaa attatagaag aattttcatt acaggggtta gaagatgcct
     1801 ccccttacag aataagtagg ggagaacgac agagggttgc cctagcctca atttttatta
     1861 atgaaccttc ctttttaatt ttggacgaac ctacaacagg tttagatata gtgagaaaag
     1921 aacaactagc aaagctctta aaaaatcttc aatcccgggg cattgggatt attgtaatca
     1981 gccatgataa agattttatt aataaaaatg ctgacagaaa gattgaactt tacgggggtg
     2041 agctgcatga aaattgatcc ccgcacaaag ttagttatgg tattttcatt atcaacatta
     2101 gctgtttttg tggtggatat acttttatta tcaattgttt taataatatc tattatagta
     2161 tctaactttt ttaaagaaga ctggggattt ttaaataggg cctgtaaatt tatcgtatta
     2221 ttggttggta ttgctttttt acaaagcata ttttcaccat ctggtgaaat attatttgcc
     2281 ataaaagact ttactttatt aacgacaggt ggacttgcta aaggaattag agttatcttg
     2341 agaatgttgg ttattattat ttcagcaaca ataatgaaag gatcaagtcc gcgtgaaatt
     2401 atccaggggt taattcagtg gaaagttcct tatgaaattg cttttatggt atccttagca
     2461 atcagattta ttcctatctt aggacaggaa gcaaaagatg cctttactgc tattcaactc
     2521 aggggaatag agattgataa attatcactg acaaaaaggt ttaaagtgta ttcatatatg
     2581 ttcatgccgg ttttatccgg agtgatccat agggcaaaag aactttcaac ttctctagag
     2641 gcaagagcat ttagagctta tccggagaga actagttata taaaattaga gttatctaaa
     2701 aatgatttac taataatgtc tgcaagtatt gtttttacaa tcaccatgtt agtcatatat
     2761 attttactga ggtgaatttt atgaaattat tttcggtttt cggaattagt ctgaccggaa
     2821 aaactacaac aattgaaaat atcattaaag agttaaagaa aaggcgatat tcgatagggt
     2881 cagttaaaga gattcattat gaagattttg ccatcgataa agaaggcact aatacatatc
     2941 ggcatagcca ggcaggggct gaacttgtca ctgcccgcgg ttataatgaa acagatatac
     3001 tttataaaga gagactatct ttgcagaaaa ttcttagaaa ttactctcag gattatgtaa
     3061 ttttagaagg agtagaaaag ggtaactttc ctaaaattat tacggccaaa gaaagcgaag
     3121 aaatcgagga aagac
//
LOCUS       DATGCJ010000104         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_57757, whole genome shotgun sequence.
ACCESSION   DATGCJ010000104 DATGCJ010000000
VERSION     DATGCJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57757"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VKN64_05190"
     CDS             complement(<1..330)
                     /locus_tag="VKN64_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA59670.1"
                     /translation="MNNTSNKEEVSNKSEKIVQIKNLKKYFEIGGGIFTRSNEVVKAV
                     DGLNFDIYQGETLGLVGESGCGKSTTGRLLLRLLEPTEGKIFFEDYELTALSGKPLKE
                     KRKDIQIV"
     gene            complement(323..1303)
                     /locus_tag="VKN64_05195"
     CDS             complement(323..1303)
                     /locus_tag="VKN64_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59671.1"
                     /translation="MENKLLDVKNLKIHFKTREGLVKAVDGIDFSVEKNEIVSIVGES
                     GCGKSVTARSIMGLIGRKKSEIVKGEIYFKGENLLDKDEESMRKLRGNKISIIFQDPM
                     TTLNPVYKVGSQIAEVSLIHENIDEKSAWEKAIDMLKRVGIPSSDKRAEQYPHQFSGG
                     MRQRAIIGMGLACEPDLLIADEPTTALDVTIQAQILDLLQDLKEETNAAIILITHDLG
                     VVAEISDKVVVMYAGMVAESAPVRKLFNNPSHPYTKGLLDSIPVPGKKERLTPIEGQP
                     PDLHDIKPGCRFAHRCDFAEGKCFEEIPPLRAIEDDHYIACWQEVDLKDE"
     gene            complement(1316..2155)
                     /locus_tag="VKN64_05200"
     CDS             complement(1316..2155)
                     /locus_tag="VKN64_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59672.1"
                     /translation="MKNIWYRFYKNKLAFAGFVILMIFVILAVFAPLIAPYDPFKMNP
                     DKMLKAPSLNHWFGTDQFGRDILSRVIYGSRISFKVGIISVGISLLIGTTMGIFAGFY
                     GGIIDGIISRIMDVMFSFPHILLALVIMAILGANLTNLMIAIGIVYIPIFGRIARGSV
                     MSIKDNLYIEAADSIGVHNFIIMWRHILPNILAPVIVQTTLSFGFAIRAEAALSFLGL
                     GVEPDVPSWGIMLNEGKDWMEMAWWVAVYPGVAATLAILSFNVLGDGLRDALDPKLKN
                     ESE"
     gene            complement(2171..3112)
                     /locus_tag="VKN64_05205"
     CDS             complement(2171..3112)
                     /locus_tag="VKN64_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59673.1"
                     /translation="MWKYILKRILIAIPVLFGISIIAFFLVRVVPGDTVTAMLGTNYN
                     PAQAEILRAKYGLDQPLVVQYIKWIGNVLKGDFGQSFFTNQPVFESIVERLPVTLELM
                     GMSLFYSLFIAIPLGTIASVKRNTSVDYGASFLGLLGVSVPNFWLGTLFILLFSLKLG
                     WFPSGGFVSLFEAPIQNLRHMIMPSIALGASVGAVVMRMTRSSMLEVLGQEYMEMARA
                     KGVKKKALITQHALKNALIPVVTVIGIQMGYLLGGSVVIEKIFSLPGVGQLALQAITN
                     RDYALMQGTVLFVASGFVIINLIVDIIYSFLNPKIRY"
     gene            complement(3193..4725)
                     /locus_tag="VKN64_05210"
     CDS             complement(3193..4725)
                     /locus_tag="VKN64_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59674.1"
                     /translation="MKKKITAILLFSILSVMLFTVGTLAQDVQKGGEIIITDLSFKDA
                     KTLDPHVASAAGSMRYIENMYNTLLKYEKGSYGKIAKDLVKEYSISDDGQVYTFTLHE
                     GIKFHNGDPLTSEDVKYSINRIIESEVRAAHFEAMEEFQIVDEQTFKIILNKPVAPFT
                     TFLAYPMNAIVNKTVVEANDGNIDSVDAGSGPFKLVEWKKDQHLKLEKFADYFKEGLP
                     YLDKVTLRPIPDETARTTALRNEEVDMILQVSPKEKMKLENQDGINVVSKPGTYWEYL
                     GLNTTEGPLSNKKVRRAIAYTVDRDQLNKMVKFGEATPLTGGPIPPGHWAYPDLEMYT
                     EPNLDKAKQLLSEAGYEDGFEAELIALPNKSQLDAAQIIKQQLAEIGISIEVRSLESG
                     VYFDKLGSKDFDMTVIGWVGFVDPDEFLFNIFHTGEKWNQQGYSNEKVDKLLEEGRRT
                     IEREERKEIYEEAVRLIAEDAPMVFLYVNPQISAFVDEVKNFDINPTVTTISLETTWL
                     DR"
BASE COUNT         1651 a         1114 c          762 g         1742 t
ORIGIN      
        1 gacaatttgt atatccttac gcttttcttt taaaggtttc ccacttaaag cggttagttc
       61 atagtcttca aaaaagatct taccttcggt gggttctaaa agccttaaca acaaccggcc
      121 cgttgttgat ttaccacaac cagattcacc gacaagacct aaagtttcac cttggtatat
      181 atcaaagttt agaccatcca ccgcttttac tacttcattt gatcttgtaa atatacctcc
      241 ccctatttca aaatatttct ttaaattttt tatctgaact atcttttcac ttttatttga
      301 aacttcttct ttattgcttg tattattcat cctttaaatc cacctcctgc caacaggcaa
      361 tataatggtc gtcttcaatt gcacgaagag gtggtatttc ttcaaaacat ttaccttcag
      421 caaaatcaca tctatgggca aacctacagc ccggtttaat atcatgtaaa tcgggcggtt
      481 gtccctcaat tggtgttaat ctttctttct taccgggcac tggaattgaa tcaagcaatc
      541 cttttgtata aggatgagaa ggattgttaa atagctttct aaccggagca ctttctgcca
      601 ccattccagc atacatcaca accaccttat cacttatttc ggctactact cccaaatcgt
      661 gggtgattaa gataatagcc gcatttgttt cttcttttaa gtcctgtaat agatctaaga
      721 tctgggcctg aattgtaaca tcaagagcag ttgtcggctc atcagcaatt aaaagatccg
      781 gctcacaggc taaacccatg ccgattattg ctctctgtct cattccaccg ctaaattggt
      841 ggggatactg ttctgccctt ttatctgatg atggtatacc aacccttttt aacatatcaa
      901 ttgctttttc ccaggcactt ttttcatcta tattctcatg tattaaggac acttccgcaa
      961 tttgtgagcc tactttatag acaggattta gggtagtcat ggggtcctga aaaataattg
     1021 aaattttatt tccccttaac ttacgcatgc tctcttcatc tttatctaac aggttttctc
     1081 ccttaaaata gatctctccc ttgacaattt cacttttctt tctaccgatt agtcccataa
     1141 tagagcgggc ggttacactt ttcccacaac cggattctcc tactatggag acaatttcat
     1201 ttttttctac tgaaaagtct attccatcaa cggcctttac caggccttcc ctggttttaa
     1261 agtgtatctt taaattttta acgtcaagta atttattttc catgtcctca tccccctact
     1321 ccgactcatt ctttaatttt ggatctaagg catctcgtag tccgtctcct aatacattaa
     1381 aactcaagat tgccagagtg gcagcaactc caggatatac ggctacccac caggccattt
     1441 ccatccaatc tttaccttca tttaacatga taccccagga aggaacatca ggttctactc
     1501 ctaatcctag aaaactcagg gcagcttccg ccctgattgc aaaaccaaaa gaaagggtgg
     1561 tttgcactat aacaggggct aatatatttg gtaatatatg tctccacata attataaagt
     1621 tatgtactcc tattgaatcg gccgcttcta tatatagatt atctttaatg gacatgaccg
     1681 aacccctagc aatccgacca aaaatcggga tatatacaat tccgattgca atcattaaat
     1741 tagttagatt tgctcctaaa atggccatga ttacaagagc taataagatg tggggaaagg
     1801 aaaacattac gtccattatc ctcgatatta tgccgtcgat tatccccccg taaaaaccgg
     1861 caaagatgcc cattgtggtg ccgattaaaa gtgaaatacc tacagatata atgccaactt
     1921 taaaagatat tcttgaacca taaatcaccc tacttaaaat atccctgccg aactgatctg
     1981 taccaaacca gtggtttaaa ctgggcgctt tcaacatttt atcagggttc attttaaaag
     2041 gatcataagg ggcaattagt ggtgcgaaaa ctgccaggat aacaaatatc ataagtatta
     2101 caaaacctgc aaaggcaagt ttgtttttat aaaatctata ccatatattt ttcaactttt
     2161 atccccctca ttaataacga attttcggat ttaaaaatga atagataata tcaactatta
     2221 aattaataat aacgaaacca cttgctacaa aaagaactgt tccctgcatc aaagcatagt
     2281 cccggtttgt tatggcctgc agtgctaatt gtccaactcc aggtaatgaa aatatttttt
     2341 caattaccac tgaacctcct aataaataac ccatctgaat accgataaca gtaacgaccg
     2401 gtattaaggc atttttaagt gcatgttggg taattaaggc tttctttttt acccctttag
     2461 cacgggccat ttccatgtat tcctgtccca atacttccaa catggaggac cgcgtcattc
     2521 tcattacaac agctcctact gaagctccta aagctataga gggcattatc atatgacgga
     2581 gattttgaat cggagcctca aaaaggctga caaaaccacc ggaaggaaac catcctaact
     2641 ttaatgagaa taaaagtata aataaagtac ctaaccaaaa gtttggaact gaaaccccta
     2701 aaagtcctaa aaaacttgct ccataatcaa ctgatgtatt tcttttcact gaagcaatag
     2761 ttcctaacgg aatagctata aatagagaat aaaaaaggct catacccatt aattctaaag
     2821 ttacaggcag cctttctaca atagactcaa aaacgggttg attagtaaaa aatgattggc
     2881 caaaatcacc ctttaaaaca ttgccaatcc acttaatata ttgtactacc aagggctggt
     2941 ctaacccata ttttgccctt aatatttctg cctgggctgg attatagtta gttcctaaca
     3001 tagcggtaac tgtatcgccc ggcacaactc taaccaaaaa gaaggctatt attgaaatac
     3061 caaataaaac tggtatcgct attaatattc tttttaatat atatttccac atataaaatt
     3121 atcacctcta gctatataat aatattggcc ggttctttat ggaaccggcc aatttctaat
     3181 taattatcta acttatctat ctaaccaggt agtttccaat gaaattgtgg ttacagtcgg
     3241 attaatatca aagtttttaa cttcatcgac aaaggcagaa atttgaggat ttacatataa
     3301 gaagaccatc ggtgcatctt ccgcaattaa acgtacagct tcttcatata tttctttcct
     3361 ctcttctctc tcaatggttc ttctaccttc ttctaatagt ttatctactt tctcattaga
     3421 ataaccctgt tgattccatt tttcgccggt atggaatatg ttaaataaaa actcatcagg
     3481 atctacaaat cctacccagc caattacagt catgtcaaaa tcttttgatc caagtttatc
     3541 aaagtaaaca ccggactcta gtgagcgaac ttcaatacta atacctattt ccgctaattg
     3601 ctgtttgata atctgggctg catctaattg actcttattg ggtaaagcta tcagttcagc
     3661 ttcaaagcca tcttcataac ccgcttcact taataactgc ttagctttgt ctaaattagg
     3721 ttcggtatac atctccaaat caggataagc ccagtgtccg ggaggaatag gtccaccagt
     3781 tagtggtgtt gcctctccaa atttaaccat cttatttaac tgatctctat cgactgtata
     3841 agcaatagct ctcctaactt tcttattaga taatggacct tctgtggtat ttaaacctaa
     3901 atattcccag taagttcccg gcttagaaac cacattaata ccatcttgat tttctaattt
     3961 cattttctct ttaggagata cctgtaagat catatctact tcttcatttc ttaaagctgt
     4021 tgtacgggct gtctcatctg gtattggtct taaagtaact ttgtctaagt atggtaaacc
     4081 ttctttaaag taatcagcaa atttttccaa ttttaaatgc tggtcctttt tccattcaac
     4141 caatttaaac ggaccactac ctgcatcaac cgaatctatg ttaccatcat tagcttccac
     4201 tacagttttg ttcacgattg cattcatggg ataagctaag aaagtggtaa agggagctac
     4261 cggtttattc aagataattt taaaggtctg ttcatccact atttgaaatt cttccatcgc
     4321 ttcaaagtgg gctgccctaa cttcgctttc aatgatacga ttaattgaat acttaacatc
     4381 ttctgaggtc agaggatcac cattatggaa tttaatacct tcatgtaggg tgaaagtata
     4441 aacctggcca tcatctgata tactatattc tttaactaaa tcttttgcaa tcttaccata
     4501 agaacccttt tcatacttaa gtaatgtgtt gtacatattt tcaatgtatc tcatcgaacc
     4561 tgctgcagaa gctacatggg ggtcaagagt tttggcatct ttaaaagaaa gatcagttat
     4621 tataatctcc ccaccctttt gtacatcctg ggctaaagtt cctactgtaa ataacattac
     4681 tgataagatt gaaaacaata aaatcgctgt aatctttttc ttcatttaat atcctcctat
     4741 ttatgaattt tctgaattaa aaattaatct actcccctgc tttttaaaat actcccttca
     4801 gaaaatttct ctaccaccac ctttttatta attatcatcc tttttaaata tgagtaatgt
     4861 tatcaagtat aattattaat catttgatta tacttattca acattacaat gtataatcct
     4921 ccctcaagaa caataatata aaatagttag ttaatataac agcgtttacc aaattatact
     4981 tgccaaatga cataaatctt cattatatat ttatgcaaaa agtataccaa agttacttcc
     5041 ctattttcac aataatcata caatttttct atataattaa tgggattata catattccca
     5101 attaaaataa tcccattttt aataaaatga gactataaac aaatataatt aaaattatta
     5161 ttggacatat gcctactaaa agtataaaat atatgaaaag aagtattgag aagggagaat
     5221 aaaaaatgat caaaattggc ctcctcggtc taggaacggt tgggtccgg
//
LOCUS       DATGCJ010000105         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_326034, whole genome shotgun sequence.
ACCESSION   DATGCJ010000105 DATGCJ010000000
VERSION     DATGCJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326034"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VKN64_05215"
     CDS             <1..300
                     /locus_tag="VKN64_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HMA59675.1"
                     /translation="WSIALLGIIFKVFFINKLKIISVIMYLAMGWLIIFAIKPLIVAL
                     STKSLVFMIIGGLFYTVGILFYALQSMGFKYSHSIWHLFVLAGSIFHFIMIYLLN"
     gene            313..1023
                     /locus_tag="VKN64_05220"
     CDS             313..1023
                     /locus_tag="VKN64_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA59676.1"
                     /translation="MQKENILKILKEDPIRNMSIIGFVENNPLKAVYNKGDTYIVLAE
                     SDHLWAYISSTKHNELKKLIKKVEVPTNYYANIEDWMLPLIKGDREVEWKLKTKRYYF
                     PADIEIKDPVHEVSNLKEEDIEVILTHSHYSEHLSHELLKQRIEEGISAAIRAEDKLV
                     AWGLTHDDKSLGFLHVIKEYRNKGYAQDIGRYLINKKRELNEPAYINIEPDNYKSISL
                     SEGMGFRYDRNISWVKLK"
     gene            1042..1959
                     /locus_tag="VKN64_05225"
     CDS             1042..1959
                     /locus_tag="VKN64_05225"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HMA59677.1"
                     /translation="MIAVIGSTNIDIVYEVDHFTKPGETQTALSLNYYFGGKGANQAA
                     AVARISENKTLFSTSVGEDGEGKEVVQNFQELKIEGYNIHQNKKTGRAFIEVDKRGEN
                     RIVITPGANQLHSPEIVEEFLNKTNQQIDYCLIQNEIPVDTIEKALSIMKKEDIKIIY
                     DPAPKEKTDIDWLVGIDFLTPNETEFKYLKDNVDIKADTFEKEALKFKEKAKIKNLII
                     KRGNKELIIVNNENGIIKVKPFKVKAVDTTAAGDIFNAGLAVALSEGLSLKEAARYGS
                     AAAAVSVTKKGAQSSIPNKDEIAEMMAGN"
     gene            1982..2482
                     /locus_tag="VKN64_05230"
     CDS             1982..2482
                     /locus_tag="VKN64_05230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA59678.1"
                     /translation="MVKLTELSLEDDQKVYDMLQDIESEENGFHNSAKGLTFREFKDY
                     LIANKRMSEGMNLKEGYVPQTIYWLWVDDKPVGMAKLRHYLNENLREKGGHAAYAVRK
                     SERGKGYGKFILKELISKAKEMDIEDLLLTVDDVNIPSRKVIEANGGELEKIKDGECY
                     YWIKNI"
BASE COUNT          960 a          339 c          520 g          687 t
ORIGIN      
        1 tggtcgatag ccttattagg tataatattt aaagtctttt ttataaacaa gttaaaaata
       61 ataagtgtaa ttatgtactt ggcaatggga tggttaatta tctttgccat taaaccttta
      121 attgtagctt tgagtacaaa aagtctggta ttcatgataa tcggtggatt attttatacg
      181 gtcggcattc tattttatgc cttacagagt atgggcttca aatacagcca ctcgatttgg
      241 cacctatttg ttcttgccgg cagcatcttt catttcataa tgatatatct attaaattaa
      301 caggtgataa aaatgcaaaa agaaaatata ctaaaaatac tgaaagaaga tccaataaga
      361 aacatgagta ttattggttt tgttgaaaac aaccccctta aagcggttta taacaaaggg
      421 gatacatata tagtgttagc tgaaagtgac catttatggg cttatatttc cagtactaaa
      481 cataatgaat taaaaaagct aataaaaaaa gtggaagttc ctaccaatta ttacgcaaat
      541 attgaagact ggatgttacc actcataaaa ggagatagag aagttgaatg gaagttgaag
      601 accaaacgct actattttcc cgccgacatc gagataaaag atcctgtgca tgaggttagt
      661 aatttaaaag aagaagatat agaggtaata cttactcatt ctcattattc cgagcattta
      721 tcacacgaac ttttaaaaca gagaattgaa gaggggatct cagctgccat ccgggcagaa
      781 gataaactgg tggcctgggg tttaacccat gatgataaat cactgggatt tttacatgtt
      841 atcaaggaat acagaaataa agggtatgcc caggacatcg gaaggtattt gattaataaa
      901 aaaagagaac taaacgaacc tgcctatatt aatattgaac ctgataacta caagtcaatc
      961 agcctatcag aaggtatggg ttttagatat gatcgaaata tcagctgggt taagttaaag
     1021 tgatttaaag ggagtgaaac aatgattgct gtaattggca gcacaaacat tgatatagta
     1081 tatgaagtcg accactttac taaaccgggt gaaacacaaa ctgccttaag tttaaactat
     1141 tatttcggcg gcaaaggggc caatcaggcg gccgcagtcg ctagaataag tgaaaacaaa
     1201 accctgtttt ctacctcagt gggagaagat ggagaaggaa aagaggtagt ccaaaacttt
     1261 caagaattaa aaatagaggg atataatatc catcaaaaca aaaaaacggg tagagccttt
     1321 attgaagtag ataaaagagg tgagaatagg attgttatca cccctggtgc aaatcaatta
     1381 cattcacctg aaatagtgga agagttttta aacaaaacaa atcaacagat cgattattgt
     1441 ctcattcaaa atgagatacc tgtcgatacc attgaaaaag cattatcaat aatgaaaaaa
     1501 gaagatatta agataattta cgatcctgct cccaaggaaa aaacagatat tgattggttg
     1561 gtaggtattg attttttaac tccgaatgaa actgaattta aatatttgaa ggataatgtg
     1621 gatataaaag ctgatacctt tgaaaaagaa gctttaaaat tcaaagagaa ggctaaaata
     1681 aaaaacctta ttataaaaag aggtaataaa gaattaataa tagtgaataa tgaaaacggg
     1741 ataataaaag ttaaaccctt taaagtaaag gcagttgata ccacggcagc cggtgatatt
     1801 tttaatgctg gtttggcagt ggcattaagt gaaggacttt ctttaaagga agcagcccga
     1861 tacggatcag ctgcagcagc tgtatcagta accaaaaagg gtgcgcaaag ttcaataccg
     1921 aacaaagatg aaattgcaga aatgatggct ggtaattaaa aaaatggagt tgaaaataaa
     1981 aatggtaaag ttaactgaac tgagtttaga ggatgaccaa aaagtatatg atatgcttca
     2041 ggatatagag agtgaggaaa atggttttca caatagtgct aaaggtttaa cttttagaga
     2101 atttaaagat tatttaatag ctaataagag aatgtcagaa ggaatgaatt taaaagaggg
     2161 ttatgtgccc cagaccattt attggttgtg ggtagatgat aaaccggtcg gaatggctaa
     2221 actcaggcat tatttaaatg agaatttgag ggaaaaaggc ggccatgctg cttatgcagt
     2281 gcgaaaatca gagcggggca aaggttacgg taagtttatt ttaaaagaat taataagcaa
     2341 agcaaaagaa atggatatag aagacctttt gttaaccgtt gatgatgtta atatcccttc
     2401 ccgcaaagta attgaagcaa acggcgggga gttagaaaaa attaaagacg gcgaatgtta
     2461 ctactggata aaaaatattt aaaatgtcag agggtgataa agatga
//
LOCUS       DATGCJ010000106         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_191978, whole genome shotgun sequence.
ACCESSION   DATGCJ010000106 DATGCJ010000000
VERSION     DATGCJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191978"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VKN64_05235"
     CDS             complement(<1..417)
                     /locus_tag="VKN64_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HMA59679.1"
                     /translation="MQGTIIFGLIFAILIAIFAIQNASIVALNILMWEFEISLAVVVL
                     GSIIFGALVIGIFSYFKQFQLKRKNKNLKLEIQSLKEELDDLKEEKRRLERYSPESSV
                     VDSSQKEESQEEKPKEKRGFFGLGKKSSKEKEAKSEE"
     gene            complement(433..1299)
                     /gene="secF"
                     /locus_tag="VKN64_05240"
     CDS             complement(433..1299)
                     /gene="secF"
                     /locus_tag="VKN64_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HMA59680.1"
                     /translation="MDILGKRRIWYAISIAILVIGLLFLAINGLNFGIDFQGGSLLEF
                     RFDQEISNQEVRNVLQKINIAPDAKIQQSDTQGIKGVLIRAQKLSPKQISDIEEGLRN
                     EYPSTDMLRSESVGAVIGQELRTKAILALLIASVAIVLYISVRFEFRFAIVSIITLMH
                     DVLITLGIFAILGSEVNTPFVAALLTIIGYSINDTIVIFDRIRENMKMMHKTPFKELA
                     NQAVVDTLPRSINTSLTTLITILAIYLFGGASIKVFMLALFIGMFAGTYSSIFVASPL
                     LVTWTHRITNKK"
     gene            complement(1305..>2250)
                     /gene="secD"
                     /locus_tag="VKN64_05245"
     CDS             complement(1305..>2250)
                     /gene="secD"
                     /locus_tag="VKN64_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HMA59681.1"
                     /translation="IVELPAVDNPNEAIETIGRTAVLTFRNPAGQVLMTGEFVKNATA
                     DYDQYGRPVVSFEMNSEGADKFENITRQYMGQKIGIYLDDELLTNPTVQSVISGKGQI
                     TGYDSVEAAQKDAILIREGSLPVPVEVVQSRTVGPALGEISIRRSIIAGLIGLALVAV
                     YMMFYYRFPGVLAAAVLVIYGILLMGTLAGLQATLTLPGIAGLILSIGMAVDANIIIF
                     ERIKDERKSGKTLRASIDAGFKRAYKTIIDANVTTLITALILAYYTSGTVRGFAVTLG
                     IGVLVSMFTAFFITRNVIDLFTHSNLLQSDAAFGAKRR"
BASE COUNT          716 a          463 c          322 g          749 t
ORIGIN      
        1 ttcttcgctt ttggcttctt tttctttact agatttttta cccagtccaa aaaagcctct
       61 tttttctttg ggcttttctt cttgactttc ttctttttga gaagaatcta caacactact
      121 ttccggagaa tatctttcta atctcctttt ttcttctttg agatcatcta attcttcttt
      181 taaagactga atttctaatt ttaaattctt attttttctt tttagctgaa actgtttaaa
      241 gtaactgaaa ataccaatta caagggctcc aaaaattatc gaaccaagta caacaactgc
      301 cagagaaatc tcaaactccc acattaatat atttaaagca actatggaag cgttttggat
      361 tgcaaatata gcaattagaa ttgcaaatat caatccaaaa ataatggttc cctgcatatt
      421 taaaacccct ttctattttt tatttgtaat cctgtgtgtc caggttacaa ggaggggact
      481 ggctacaaat atagaagaat atgtaccagc aaacataccg ataaataatg caagcatgaa
      541 tactttaatg gaagccccac cgaataaata tatagctaat attgttatta aagttgttaa
      601 agatgtattt attgatcttg gtaaagtatc tacaacggcc tgattagcca gttctttaaa
      661 gggtgtcttg tgcatcatct tcatgttttc tcttattcta tcaaagataa ctattgtatc
      721 atttatagaa taaccaataa ttgttagtag tgcggccaca aagggtgtat ttacctcact
      781 gcccaaaatg gcaaaaatac caagtgtaat taaaacatca tgcatcaacg taattattga
      841 aactattgcg aacctaaatt caaaacggac acttatatat aagacaatag caacagaagc
      901 tatcagtaaa gcgagtatag ctttagttct taactcctga cctataactg ccccaacgga
      961 ctcagatctc aacatatcgg tagaaggata ttcgtttctt aagccttctt ctatatcact
     1021 gatctgctta ggtgacaatt tctgtgctct aatgagaaca ccttttatac cctgagtatc
     1081 agactgctgg atcttagcat ccggagcaat atttatcttt tgtaatacat ttcttacttc
     1141 ttgattgctt atttcttggt caaatctaaa ttctaataac gaaccaccct gaaaatctat
     1201 accaaagtta agtccattta tagctaaaaa taatagtcca attactagta ttgctatgga
     1261 aatagcatac caaattcttc tttttcccag tatatccatc ttctttacct cctctttgca
     1321 ccaaatgcag catcgctttg tagtaaatta gaatgagtaa ataaatctat aacatttcta
     1381 gttatgaaga aggcagtaaa catacttact aatacaccta ttcccaatgt aactgcaaat
     1441 cctctcactg taccacttgt atagtaagca agaattaaag cagtaattaa ggtggttaca
     1501 ttagcgtcaa taattgtctt atatgccctc ttgaaaccag catctatgga agctcttaag
     1561 gttttaccgg acttccgttc atcttttatt cgttcaaaga taataatgtt ggcatcaaca
     1621 gccataccta ttgataagat aagtccggcg atacctggta aggtaagggt agcttgtaaa
     1681 cctgccagtg tacccattaa taaaatccca taaattacta aaactgcggc tgcaagtaca
     1741 cctgggaatc tataataaaa catcatatat accgccacta aagccagccc tattaaacca
     1801 gctattatac ttcttcttat cgaaatttca cccaaggccg gtccaactgt cctactctgc
     1861 actacttcaa ccggaacggg taaggaaccc tctctaatta aaatagcatc cttttgagcg
     1921 gcctcaacag aatcataacc tgtaatctga cctttaccag aaataacact ctgtacagtt
     1981 ggatttgtta ataattcatc atctaaatat atacctattt tttgtcccat atactgtctg
     2041 gtaatatttt cgaacttgtc tgcaccttca ctattcatct caaaagaaac aactggtcta
     2101 ccatattggt cataatcagc agttgcattt tttacaaatt cacctgtcat caatacttga
     2161 ccggctggat ttctgaaggt caagaccgca gttctaccga ttgtttcaat tgcttcattt
     2221 ggattatcaa cagcagggag ctctacgatt
//
LOCUS       DATGCJ010000107        19340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_57930, whole genome shotgun sequence.
ACCESSION   DATGCJ010000107 DATGCJ010000000
VERSION     DATGCJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 19340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19340
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57930"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VKN64_05250"
     CDS             <1..962
                     /locus_tag="VKN64_05250"
                     /inference="COORDINATES: protein motif:HMM:NF021622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59682.1"
                     /translation="YNIQSFEFGEGLIQSDSLQDFYDRLASLLSKRILARARKGFNKS
                     YHNQNRKLNYIRGRIDSSNLVSKTWEVKFNCLYQLHLADNKENKILLWTLYKIIKNGI
                     CSPDTISTVRKAFRSLQSFAQLESYSAKDCLDINYNRLNQDYEILHALSRFFLSERGP
                     TYEAGTNRMFPFLIYIPSLFEMFIAEWLNQKLPDTHDIKSQEKFSIDKDRNIFFKMDI
                     VLYNSFDKSVTCIIDTKYKNTKSPSMSDISQIVTYALSKNCDEAILLYPTDEIDEIDQ
                     KLNNIRIRSLSFALDDNLDKEGNIFLKKIILKKGGTYISEFKN"
     gene            982..2283
                     /locus_tag="VKN64_05255"
     CDS             982..2283
                     /locus_tag="VKN64_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMA59683.1"
                     /translation="MKKTPNLFDYAQKELSHDAFFCWLADCANSNIKEYNDIGKRFLN
                     LLIDKISLEKDSFNDLNLDDKNIEVFNQKSNMDIIITIKPRNNKNNIMWVILIENKIK
                     SKQHSGQLVRYREKITNDYDIILDSKDYILDEKNLGCVYLRPEYQANYLPVLASGFLP
                     IHGNELLDIFEDTKLVEEDTLINSYYRYLKEKINRKSWVDLYREILKDSKFRKVILNG
                     SENRLEYLKNKYDTESKEYIFLDQLKEKEYNEFVEEGYFLDKHYVWVNNQSGGFPCLY
                     FGEIIYEPDSYQSLKFKGDRRIRIKLQFESYNKGKNMRVGAVVKTIKKSHIDYGIVKG
                     INKDEVSNLQTNIRKELWDKAQNYNKIKDQLFVRPPRFGHGGHMTFAVLKDLDNIEID
                     KDKIKDNDINIYNNGNKIAFDDVIDILIEGVKGLEYINEEL"
     gene            2296..2580
                     /locus_tag="VKN64_05260"
     CDS             2296..2580
                     /locus_tag="VKN64_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59684.1"
                     /translation="MLNEIKNILDLCDTRESVIPPTELYNEGWLLRLLINWFSKSHNV
                     SHELAFIDQDTRWYSELLLPSQFLARSRGDKLAESYTHADGLIGHFKIGN"
     gene            2641..3060
                     /locus_tag="VKN64_05265"
     CDS             2641..3060
                     /locus_tag="VKN64_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59685.1"
                     /translation="MNSQLSKGVTNASYFDQASRNVACIAEVLNKSNIDIDQIDSLGF
                     YVIAPEEKLNEKTNLTKYTDEDNVDNKVKRRVNEYEGESGKRKGEWYNKWFLPTKNKA
                     KIECLSWEEVIRFIKKNDKNYGKDLNVFYKKCLKYND"
     gene            complement(3635..4246)
                     /locus_tag="VKN64_05270"
     CDS             complement(3635..4246)
                     /locus_tag="VKN64_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59686.1"
                     /translation="MKKLLSLLIVTLFVFSITFVGSTMYLPESIDDDMIENSIEVAKE
                     SETPEDLIVDNGYRFDLGGSISGKGMGSVDVLTPRFDLLELAFKKAKKYKEPKEEEIK
                     TILNDETFKLMSFLASEDKPYEDDLHIVIKKQNEEIVQPVDFNLQFVDYDMDSDLYFS
                     YQVAVFNLDELNSNNGQINETIEVILISNLGEIKGKVDLSSVK"
     gene            4905..5195
                     /locus_tag="VKN64_05275"
     CDS             4905..5195
                     /locus_tag="VKN64_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59687.1"
                     /translation="MKKGHFYIYNPIRNRREPEFYVNNIKVEKDKIEISIEGDYRKIE
                     WIAYDSVSKKNEVFAEGKRFQISDLSYKSTYIRAKITSKAGDILTQPFGIVY"
     gene            complement(5257..6198)
                     /locus_tag="VKN64_05280"
     CDS             complement(5257..6198)
                     /locus_tag="VKN64_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMA59688.1"
                     /translation="MSNREKLAVGADIGGTKTNIAIITKNGSIKELKKIQTTAENPEA
                     IKDSLIKNITSLINGYDNIIGIGIASAGRVIFKKKIIGYATDNLKDWTEYPLVEQLKN
                     EFELPVFIDNDVNAALLSEIKISDSLMEKYNNIVFITIGTGLGGALAIDGKIIRGSTG
                     SAGEFGHMLLYPGGKPCNCGKYGCAEQYISGKAFKKRLKEKFKENLVSFDKGDLKDDI
                     IQREIKRDNVLYRSTLREMTEDLALLLENLKNSFDFELCIFGGSFSAYSEIMLEIIRK
                     EFEKYNHKYYKNPDFIFSEMGNKAGVIGAGLLVFEQI"
     gene            complement(6219..7076)
                     /locus_tag="VKN64_05285"
     CDS             complement(6219..7076)
                     /locus_tag="VKN64_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HMA59689.1"
                     /translation="MNMNNNKGVIPRIRSEYLSLPPSEKKVADYVLDNPDKIIYLSVS
                     ELADEVDVSDSTIIRFCKNVGFKGYQEFKLFVAQDLVVPIENINEDIQLDDDLETIVN
                     KIGFTNKKAIEETLSITNLKALQEAIDAIVKARKLEFYGVGASGITGHDAKYKFLRIG
                     KDVDCYTDAHLQAMSAATLSDQDVVVGVSHSGSTKDVVDSCTVAHEAGATVICITGHN
                     RSPITKVADIKLLTATRERPLASGALRSKIAQLQVLDMLYTGVSLQIQDQTMEYTEKT
                     AKAVLDKLY"
     gene            7406..8563
                     /locus_tag="VKN64_05290"
     CDS             7406..8563
                     /locus_tag="VKN64_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HMA59690.1"
                     /translation="MQLKPTFTDYINGVISDFEEQNPDVKVKWVDVPWADMEKKILSA
                     TAANNAPDVANLNVPFSMKLAELDALVDMDSNLDQETIDKYFPTVWEANTFEGETHAI
                     PWYLSTSLTMYNTEIFKEAGLDPNNPPQYYDELLEMGRTVKEETVKYLLFPPIAETGR
                     YLEYMVREGIPLTNEDGTAAFNTEKSVELLQMWTTMYEEGIIPEETVTEGHNAAVDVY
                     QSGQAAILLTGPQFLSRIEENAPEVFEVTEVAPEISGNADRVGVAVMNIAVLEQTEHK
                     QAALDFAAFITNNKNQLDFAKIVNILPSTKEAAQDPYFTDVDEGSVKDRAKKVSAQQL
                     DRATNLVPPMENQGELNEVINQMVQNALLGEKTPAEALNDAEAEWNNIVNN"
     gene            8627..9487
                     /locus_tag="VKN64_05295"
     CDS             8627..9487
                     /locus_tag="VKN64_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA59691.1"
                     /translation="MKLRKTYTPYLFLIPALIFMGIFLFYPIVDVFRLSFTDYNMITE
                     AKFLGTQNYENLFNDPLFWKTLKNSFIYLIGVVPLLVILPIFIAVLVNRQLKGIKWFR
                     AAYYIPVVTSMVVVGIMWKWLYKGNGLLNYLLMSIGLINDPISWLTSTKYALYSVMAV
                     TIWKGLGYYMVIYLAGLQSIPEELYEVSKIDGANWWQKHLYVTIPLLKPSILLVTILS
                     SIAAMKVFTEVYVMTKGGPINSSKTLVFYIYEKAFENLNLGYAAAMGFVLFIVIFILS
                     YFNFKILGQE"
     gene            9506..10342
                     /locus_tag="VKN64_05300"
     CDS             9506..10342
                     /locus_tag="VKN64_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA59692.1"
                     /translation="MFNKYIDFLKTLLLYILLSVIAIITIGPFLWLLSTALKSGGENI
                     FSYPPSFIPKNPTLINFKKVWNAINIGRFFLNSTFVALATIVINIFVSALAAYPLARM
                     KFKFKNIIFYGILGTMMVPFQILMISIYILSVRTGMTDSYAGLILPVAVNAFGIFLLR
                     QAFITIPVDIEEAAIIDGCNSFQIWYKILLPLIKPSIATLAIFTFMMSWSRFMWPLIV
                     INNPDYYTLPVGISYLSGLFSSNWRLIASGSLISIVPIIILFLFLQRYFIKGAMAGAV
                     KG"
     gene            10349..11704
                     /locus_tag="VKN64_05305"
     CDS             10349..11704
                     /locus_tag="VKN64_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA59693.1"
                     /translation="MKDTLTLKENINIIDHYDVIVLGGGTAGSVAGSAAARLNAKTLI
                     IESEGALGGTSTVGQVTPMMPVEIEGNPDSSAFNKEIKKRLIAEGYGAKDSNGNDGWF
                     NPEMLKHTLEDIYLEYNGDILYDCEFVKPIVENDTIKGVIVYNKNGLEAYTADQFIDA
                     TGDALVAYRADVPCKEGSKEDKYQAVSLRFTVSNIELKKFTNFLSELGQDYGLEYPLV
                     ETAMVWGEGFALEKIFLEALESGDLKKEDGKYFQAFSIPGMPTSMYFNCPEIPGKINS
                     LDPKELTEARIKGRKMMKRLYNFFVDYLPGFNTSFIASEASIIGIRESRRIVGEYVLD
                     SEDYQKRRKFPDAIANSAYPIDIHGDELELKEVKKGEYYQIPYRTLIPKNITNLLVTG
                     KSISSTFTTQAAIRIQSICRATGEAAGIAAAMAVKENITVRDIDGKKIKEQMLNWGAD
                     I"
     gene            11726..13261
                     /locus_tag="VKN64_05310"
     CDS             11726..13261
                     /locus_tag="VKN64_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HMA59694.1"
                     /translation="MSDKIMFIPLDERPCNYKYPELIYKITLDNLIKPPLSILGNKKE
                     PVAVDKLQKWILNNLEGISHLILSVDMLVYGGIIPSRIHQLDEKEIFSRLKFIEKIKD
                     INPDMKIFAYNLITRVPSYNSDDEEPDYYKYHGADIAKYGYYSDLIKQKLAEKKQKEE
                     FEEIKSNIPDKVINDYLNRREKNHALNKKVIDYVENNLIDFLIFPMDDNSEYGFCAKE
                     RRKIIEKVYKKELLDKIYSYPGADEVGTVLTARTFLDKNNYRPKVYIKYSSEKGRNII
                     PYLEDRPLEQTVKYQILAANGIVVDNSQEADYVLVVNTPTKKILNSIKGWQSILNMDD
                     MIDPTRNLNEVVESINYYLSKDLPVAVADVAVVNGSDQYLMQLLKNYNLITKLSAYGA
                     WNTSSNTIGSVVAHANILNYYQKDNSLTDKQKDLSNRYLFLRYLEDWGYQHVVRTEVN
                     NKLDEYGLEYFNLKDKTDFISNLIKDKLYKFRNENLDKFSYDFTIDMPWNRMFEVDIK
                     IEK"
     gene            13288..13953
                     /locus_tag="VKN64_05315"
     CDS             13288..13953
                     /locus_tag="VKN64_05315"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HMA59695.1"
                     /translation="MKNLKENSLIVSCQALADEPLHGSEIMAKMALAAKRGGASGVRA
                     NSPEDIKAIKNKIELPIIGLWKNKLINYEVYITSKYQHAKAVLKAGADYVAIDCTDRR
                     RPEPLSEIFNKLRTKFPDKGIVADISSTEDFKDIEELAPDYISTTLSGYTEKTQNRTR
                     PDIDLVEKLNMISNIPILAEGNYKNGKQAKKALKAGAYAVVIGAAITRPQVITAHIIE
                     NMN"
     gene            13974..17159
                     /locus_tag="VKN64_05320"
     CDS             13974..17159
                     /locus_tag="VKN64_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HMA59696.1"
                     /translation="MRDFLKEINNKMNHKIKDIKNWQAREAIYKETGKYKFISDWDNF
                     QLGDSWSEEEEITTFFKTKVFIPEIDFENKLELNIETGGEAALYINDELIQGLDRNHS
                     YVTISKDYSSKEVELFIEAALDRINKIYVPSPDYKHVLNKAALLSVNKSAETFYYNIL
                     SLIELIETLEERNKKDVLVQLKDIFSTLKEDYRSYPKSKIDFNILNKKYSKLINNIDY
                     DTTYKIACFGHAHIDVAWLWQFKETVRKSARSFSTAVNLMEEYPEFKFIQSQPKLYQY
                     MKEYYPNLYEKIKDKIADKNWQVEGGMWVEADTNLLSGESLVRQFLYGKKFIREEFNV
                     ESKVGWLPDVFGYTASLPQIMKKSEVDYFMTSKISWNDTNNFPYSIFRWEGIDGTDVL
                     THVPRVLLPYTYNGEVYADKILKVRDNYEMQKETEIEEFKYGDSSNYFKQDRVIPDDQ
                     LIYIYGYGDGGGGVTPSFINKIKRFDKLPYMPKLEFAQPREYFENLEKDMQINNREYP
                     TWKGELYLEYHRGTYTTQAEIKRQNRYLEDNIREAEILNTITERYPQNKFENLWKKIN
                     LNQFHDIIPGSSIKEVYEDVNQIYAEVTDEINNILKNNLSSLGNNIEIKSKDNSKYYL
                     IWNGLDYKRSSYIKFSTEEFDKDIEVFDYITGKKLKNNSKEDLKEKKLFEIYIEDVPP
                     FGYRIIEIKENKEPNNDMTSKLNKNNIDKTLENSYYKIKFKEGLITSLFDKRREVELI
                     KEGKVGNELQFFVDKPDKYEAWEQDADFEEHRIYDELELDSFEIKEYNTQKVAQLKWN
                     FRKSVFKQNILIYDNLDRIDIKNKVAWRDREILVKAAFPLNILSNEARYEIAYGNITR
                     PTTNNTSYEKAQFEVPGHRWADLSEPGLGFSIINDCKYGYDIKGSLMRLTLLKSPNYP
                     DETADYGIHEFTYSLYTHDGIYYNSNLLKHADDLNRPFLTKNIEINNDVKDNSNLSYS
                     FAKIIKGNSRLEVIKRSEQNPDKVILRFFEPYGGKDKVEVELTKEIKNVETVNLIENK
                     INDKVKYEKNNLNFEMDPFEIKTIKLELIDN"
     gene            17178..17903
                     /gene="nagB"
                     /locus_tag="VKN64_05325"
     CDS             17178..17903
                     /gene="nagB"
                     /locus_tag="VKN64_05325"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HMA59697.1"
                     /translation="MRVLIEKDYNEMSKKAAYLVKKQINDKPDSVLGLPTGSTPLKMY
                     KVLIEMYRRGEVDFSEVTTFNLDEYYGLPFDHSQSYHYYMWNNFFDHINIKDENVHIL
                     DGMTENIDQERIEYETKIKNAGGINLQVLGIGPNGHIGFNEPSETLNDKTHLVTLTEE
                     TRESNSRFFDSKDEVPKKALTMGISTILKADKILIMASGEGKASAIKKTLSGQINTCT
                     PSTLLQTHSNVTVVIDQGAAIYL"
     gene            18240..19094
                     /locus_tag="VKN64_05330"
     CDS             18240..19094
                     /locus_tag="VKN64_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMA59698.1"
                     /translation="MWAENNFQQLSFDDILLNMPKYLKNFLKDSWADVFQEIIFPAID
                     EKRFSVLYSDKPSRPNSPVNVIIGSLILKEIFQLSDKELLGAIYFDDRFQYALRLTSE
                     EKPPISINTFSNFRRRVYEFEQETNRDLIKEEVKSISNLIAKKLKIDNKKVRVDSFMF
                     ASSCRNLSRLELIYTVNRQMAKKLKQEDEDIIPHGFHQYLKDGHKKNTIYTTHDNEAE
                     SKLEFLLKHSKTFYDLILESSLDIYKSQEFKNLKRLLNKQTKSNDKDDDDNNYTPKEN
                     GEIKPDSL"
BASE COUNT         7294 a         2688 c         3141 g         6217 t
ORIGIN      
        1 cttataatat acaaagtttt gaatttggag aaggattaat acaatcagat agtttacaag
       61 acttttatga taggttagca agtttacttt caaaaagaat attggcgaga gcccgtaaag
      121 gttttaacaa gtcataccat aatcagaata gaaaactaaa ttacataaga ggtcgtattg
      181 actccagtaa tctagtatct aaaacatggg aggttaagtt taactgtctt tatcaactcc
      241 atttagctga caataaagaa aacaaaattt tattatggac actttataaa attataaaaa
      301 atgggatctg cagtccagat acaatttcta cagtcagaaa agctttccgt tctttacaga
      361 gttttgcaca attagaatct tattcagcca aagattgttt ggatatcaat tataatagac
      421 tcaaccagga ttatgagatc cttcatgctc tttcaaggtt tttcttaagt gaaagaggtc
      481 caacttatga agctggtact aatcggatgt ttcctttttt aatatatatt ccttctctat
      541 ttgaaatgtt tatcgcagag tggttaaatc aaaaattacc tgatactcat gatataaaat
      601 ctcaagaaaa atttagtatt gataaagata gaaatatatt tttcaagatg gatatagttt
      661 tgtataattc atttgataaa tcagtaactt gtataattga tacaaagtat aaaaatacta
      721 aaagtccttc tatgagtgat atatctcaga ttgttactta tgcattaagt aaaaattgtg
      781 atgaagctat tcttttatat cctactgatg aaatagatga aattgatcag aagttaaaca
      841 atataagaat aagatcattg agttttgctt tagatgataa cttagataaa gaaggaaata
      901 tatttctcaa aaaaataatt ttaaaaaaag ggggaacata tatatcggaa tttaaaaatt
      961 aataaatatc cgatataaat tatgaaaaaa acaccaaatt tatttgatta tgctcaaaaa
     1021 gaattatcac atgatgcatt tttttgttgg ttagccgact gtgcaaacag taatattaaa
     1081 gaatataatg atattggtaa aagattttta aatttgttga ttgataaaat aagtttagag
     1141 aaagattcat ttaatgatct aaatttagat gataagaaca ttgaagtttt taatcaaaaa
     1201 agtaatatgg atattataat aactataaaa ccaagaaaca ataaaaataa tataatgtgg
     1261 gttattttga tagaaaataa aataaaatca aaacagcatt ctggtcaatt agtaagatat
     1321 agagagaaaa tcaccaatga ttatgatatt attttagatt ccaaagatta tatattggat
     1381 gaaaaaaatt tgggttgtgt atatctccga cctgaatatc aggctaacta tctacctgta
     1441 ttagcatctg gatttttacc aatccatggg aatgaattgt tagacatttt cgaagataca
     1501 aaattagttg aagaagatac attaataaat tcatattata gatatctaaa agaaaagatt
     1561 aatagaaaaa gttgggtaga tttatataga gaaatcctaa aagacagtaa atttagaaaa
     1621 gttattttaa atggttcaga aaaccgatta gaatatttaa aaaacaaata tgatactgaa
     1681 agtaaagaat acatattttt agaccaactg aaagaaaaag aatataatga attcgttgaa
     1741 gaaggatatt ttcttgataa acactatgtt tgggttaata atcagagcgg aggattccca
     1801 tgtttatatt ttggggagat catttatgaa cccgatagtt atcaaagcct taaatttaaa
     1861 ggagatagaa gaataagaat taaactccaa tttgagagtt ataataaagg taaaaatatg
     1921 agagtaggag cagtagtaaa gactattaaa aaaagccata ttgattatgg tatagtaaaa
     1981 ggaattaaca aagatgaagt tagtaactta caaactaata ttagaaaaga attatgggat
     2041 aaagcccaaa attataataa aattaaagat cagttatttg tgagacctcc aagatttgga
     2101 catggtggcc atatgacttt tgctgtactc aaagacttag ataatattga aatagataaa
     2161 gataaaatta aagataacga tattaacatt tacaataatg gaaataaaat agcttttgat
     2221 gatgtgattg atatattaat tgaaggtgta aaaggtttag aatatataaa tgaggaatta
     2281 taagggagta gagaaatgtt aaacgagatt aaaaacatat tagatttatg cgataccagg
     2341 gagagtgtaa ttccacctac tgaattatat aatgaaggat ggcttttaag acttctcatt
     2401 aattggtttt ctaagtctca taatgtttca catgagttag cttttattga tcaagataca
     2461 agatggtatt cggaattatt gttaccttca caattccttg ctagaagtag aggggataaa
     2521 ttagcagaaa gttatactca tgcagatgga ttaattggtc attttaaaat tggtaattaa
     2581 ggtaaaggag atatactact taacgaagac gctaaacaat ttatagtttt ggaggcaaaa
     2641 atgaatagtc aattatcaaa aggtgttaca aatgcaagtt attttgatca agctagtaga
     2701 aatgtagctt gtatagctga agttttaaat aaatccaata tagatattga tcaaatagat
     2761 agtttaggat tttacgtaat tgcaccagaa gaaaaattaa atgaaaagac aaatctaacg
     2821 aaatatacag atgaagataa tgtagataat aaggtaaaaa gaagagtaaa tgagtatgag
     2881 ggtgaatcag gtaaaaggaa gggagaatgg tataataagt ggtttttacc aaccaaaaat
     2941 aaagctaaga ttgaatgttt aagttgggaa gaggtaataa gatttattaa aaagaatgat
     3001 aaaaactatg gtaaagattt aaatgttttt tataaaaagt gcttaaaata taatgattaa
     3061 atttttcata ataataatca tctaggaaaa atacaggtat gcctaataat atcaaaacaa
     3121 gtaaaaatga gtttcctttt caacttaaca cttacagaat ttttaactaa agatctcttt
     3181 aagctcaaat ttttatagta tttcctatag agatctatta agaatataat caagcaaagc
     3241 aatctttcgg agtccatcca cattaaaata taatttacat tttttaaaaa aatatagtga
     3301 tatgagagta acacataaac ttcatctttc atatatagga acttgattat ttttaattga
     3361 gactgatctt agtgggtaat tttccagata aaaaagtatt taaattataa cgattgatga
     3421 aatttttaaa atattaatac aagaaataaa tttgttatga ggtgatctct ccaaaaaaaa
     3481 tgtgaaaata gcaaatccat ctatattaaa attattagga ataatagttg aaataacaga
     3541 aatattgaat ttaaatttaa taaaaaaata tgaatagaag tggttagcat taatcacttc
     3601 tactcattaa aatgcttgtg attttgagtt tgttttactt aacagaactc aaatctactt
     3661 taccttttat ttcacctaag ttagatataa ggataacttc aatggtttca tttatttgtc
     3721 cattattact atttagttca tcaagattaa atacagccac ttggtaacta aaatataaat
     3781 cagaatccat gtcataatcg acaaattgta gattaaagtc tactggttga actatctctt
     3841 cattttgttt ttttataact atatgaaggt catcttcata cggcttatct tcacttgcta
     3901 aaaaactcat taatttaaaa gtttcatcat ttaatattgt tttaatttct tcttctttag
     3961 gttctttata tttttttgct tttttaaaag ctaactctaa taaatcaaac ctgggtgtta
     4021 atacatctac agaccccatt ccttttccac ttatagaacc tcccaagtca aatctataac
     4081 cattatctac tatcagatct tcgggtgttt cagattcttt agccacttct atactatttt
     4141 ctatcatatc atcatcaata ctttcaggta aatacatagt tgagccaaca aaagtaatac
     4201 taaagacaaa taaagtaaca attaataagg ataataattt tttcatattt tatcactcca
     4261 ataatgatat gtaatagata ccagctcttt aattagggct gttattttct attaaagaag
     4321 gaaatagggt ttgtagctaa agatacttgt gatgtgttag ggatagtaga gcacagacaa
     4381 tcaatcagag gattagatga tgaaaagggt gcaagtatta tttgtacccc tgcaggaaaa
     4441 taaaaacaat taatagttaa cgaacctagc atgtataccc ttgtttttaa atctagaaaa
     4501 cctaatgcta aaaaatttaa gcgttggccg acatgttaag taatattttt atttagagaa
     4561 aaagtttcta agctaacaag cagatgtaaa tctaataatt cgagataaat tgttaaaaaa
     4621 taaaataaaa gtaaaaaaat agtaaaatat ttttttaaac tatttaatca ttatctattc
     4681 tgtggtataa tagtattagt gaggttaaat actgttataa caaggattaa agaaatttaa
     4741 gtataagcaa aattgatcgg ttaattgata aaacaaaaat tcaaatcatg ataaattaat
     4801 aattaaataa aaaaagtcca catatggtgt tgtcgcacct gtgaagtggt cattataata
     4861 tctttttatt aaaagaaaat acagctcaaa aggttaaaga agcaatgaaa aaaggtcatt
     4921 tctatatcta taatccaatc agaaatagaa gagaaccaga attttatgtt aataatataa
     4981 aagttgaaaa agataaaatt gaaatctcaa tagaaggtga ttatagaaag attgaatgga
     5041 tagcttatga cagtgtgagt aaaaaaaacg aagtttttgc agaaggaaag aggttccaaa
     5101 tttctgatct ttcttataaa tctacttata tcagagcaaa gattacaagt aaagcaggag
     5161 atatactcac ccagcctttt ggtatagttt attaaaaaga aatagtttga ataacaaaat
     5221 tggtaaaaac ttttagatat ataataattt tatttattat atttgttcaa agactaagag
     5281 acctgcacca attactccag ctttatttcc catctcagaa aatataaaat cagggttttt
     5341 ataatattta tgattatatt tttcgaattc ttttcttata atttctaaca taatctcact
     5401 atatgctgaa aaactaccac cgaaaataca gagctcaaaa tcaaaactgt tttttaagtt
     5461 ttctaatagt aaagcaagat cttcagtcat ttctcttaat gtagatctat ataagacatt
     5521 atcccttttt atttctcttt gaataatatc atcctttaga tcacctttat caaaggaaac
     5581 aaggttttct ttaaactttt ctttcaatct ctttttaaac gctttccccg aaatatattg
     5641 ttctgcacag ccgtatttcc cgcagttaca gggtttaccc cctggatata ataacatatg
     5701 accgaactcc ccagctgaac ctgtactacc gcgaattatc ttaccatcga tagctaatgc
     5761 tccacctaat ccagtcccga tcgttataaa tactatatta ttatattttt ccattaatga
     5821 atcacttatt tttatctcag acaaaagggc agcatttaca tcattatcaa taaaaacagg
     5881 cagttcgaat tcatttttta attgttcaac aagtgggtat tctgtccagt cttttaagtt
     5941 atcagttgca taaccaataa tttttttctt aaaaattacc cgaccggcag aagctatacc
     6001 aattcctata atgttatcat atccatttat tagggatgtt atatttttaa ttaaactatc
     6061 tttaattgct tctggatttt cagcagttgt ttgaatcttt tttaactctt ttattgaacc
     6121 gtttttagtt ataatagcga tattagtctt agttccacca atatctgcac ctactgccag
     6181 tttttctcta tttgacatta tagcttcccc ctaactgttt aatataattt atccaggaca
     6241 gctttagctg ttttttcagt atattccatt gtttgatctt gaatctgtaa ggatacaccg
     6301 gtatatagca tatccaatac ttgcagttgg gcgatcttgg aacgtaacgc accactggct
     6361 aaaggtcttt cacgagtagc agtcaatagt tttatatctg caacctttgt aatcggagat
     6421 ctattatgtc cagttataca aattacagtt gccccggctt catgtgcaac cgtacaggaa
     6481 tcaacaacat ctttagtact accagagtgg gaaacgccca ccaccacgtc ttgatcagat
     6541 aaggtagcag ctgacatagc ctgaagatgt gcatcagtat aacagtctac atctttacca
     6601 attcttaaaa acttatattt tgcatcatgc ccagttattc ctgaagcgcc tacaccataa
     6661 aattcgagtt tgcgggcctt tacaatcgca tcaatagcct cttgaagggc tttaagatta
     6721 gtaatactta gagtttcctc gattgccttt ttattggtaa aaccaatttt gttaactatt
     6781 gtttctaaat catcatccag ttggatatct tcattaatat tttcaatggg aactaccagg
     6841 tcctgagcta caaatagctt aaactcctga taacctttaa accctacatt tttacagaat
     6901 ctaataatag ttgaatcact aacatcaacc tcatctgcaa gctcacttac tgacagatag
     6961 attattttat caggattatc tagtacataa tctgcgactt tcttctcgga gggaggaaga
     7021 gagagatatt cggatcttat gcgaggtatt acacccttat tgttattcat attcattatt
     7081 ttcaccccaa ataattattt aattgtatat aatgaattat aagtaatttt aaattcaaag
     7141 tcaatgaaaa ttcggaattt tattcctgta taatcttgac attcggaaaa ttactacata
     7201 taattaactt acaatgattc ttatttcact tagggggtga attgtaagaa aagtattttt
     7261 attcattatt ttcaaaaaaa tttaaaggaa ttaaagggag gatattatgt ttaaaaaatt
     7321 aattacgtta tctttattaa tcgtttttct agttagtatg tcaagtttag caatggccca
     7381 ggaaaaagaa attgaattct ggactatgca gttaaaacca actttcactg attatatcaa
     7441 tggtgtaatt tctgattttg aagaacaaaa tcctgatgtt aaagttaaat gggtggatgt
     7501 cccttgggca gacatggaga aaaaaatatt atctgctacc gcagctaata atgctccaga
     7561 tgtggccaat ctaaatgttc ctttcagtat gaagttggct gaattggatg cattagtaga
     7621 tatggactct aatttagacc aagaaactat cgataagtat tttccaaccg tatgggaagc
     7681 aaatactttt gaaggtgaaa cccacgcaat accttggtat ttatcaacaa gtttaacaat
     7741 gtataatact gaaatcttta aagaagctgg tcttgatcca aataacccac cacagtatta
     7801 tgatgaattg ctcgaaatgg gtcgcactgt taaagaagag actgttaaat atcttctatt
     7861 tcccccaatt gctgaaactg gccgttattt agaatatatg gtccgtgaag gaatccccct
     7921 aacaaacgaa gacgggacag ctgcctttaa tacagaaaag tccgttgaat tgttacaaat
     7981 gtggacaaca atgtatgaag aaggcatcat tccagaagaa actgttactg aaggtcataa
     8041 cgcagctgtt gatgtatatc aatctggtca agctgcaatt ttattaactg gtcctcagtt
     8101 cttaagcaga attgaagaaa acgctcctga agtatttgaa gttacagagg tagcacctga
     8161 aatctcaggt aatgctgata gagttggagt tgctgtaatg aatatcgcag tacttgaaca
     8221 gaccgaacac aaacaagcag ctttagattt cgctgctttt attacaaaca ataaaaatca
     8281 attagacttt gctaagattg ttaatatctt accatcaact aaagaagcag ctcaagatcc
     8341 atactttaca gatgtagatg aaggttctgt taaagacaga gctaaaaaag ttagtgctca
     8401 acaattagat agagctacaa acttagttcc tccaatggaa aaccaaggag aactaaacga
     8461 agttattaat cagatggttc aaaatgctct attaggtgaa aagactcctg cagaagcttt
     8521 aaatgatgca gaagcagagt ggaacaatat agtaaataac taatatacaa gtaacaggta
     8581 tcgcactaat taagtgcgat acctgtaatt tataggagag atatttatga aactaagaaa
     8641 aacatatact ccatatcttt ttttaatacc tgctttaatt tttatgggaa tatttttatt
     8701 ttatcctatt gtagatgtct ttagacttag ttttacagat tataatatga ttactgaagc
     8761 aaaattttta ggtactcaaa attatgaaaa cttatttaat gatccactgt tttggaaaac
     8821 cttaaaaaat tcttttatat atttaatagg agtagttccc cttttagtta ttttaccaat
     8881 ttttattgct gtcttagtta acagacagtt aaaaggtata aaatggttta gggccgccta
     8941 ttatatcccg gttgtaacct ccatggtagt agtgggaatc atgtggaaat ggttatataa
     9001 aggtaatgga ctcttaaact atttgttgat gtctatcggc ttaataaatg atccgatcag
     9061 ttggttaacc agtacaaaat atgcccttta ttcagtaatg gctgttacaa tatggaaggg
     9121 cttaggatat tatatggtta tctatttggc aggcttacaa agtatccccg aagaactata
     9181 tgaagtttcc aaaatcgatg gggcaaattg gtggcaaaaa cacttatatg ttacaatccc
     9241 tttattgaaa ccaagtattt tacttgtaac aatattatca tcaattgctg ccatgaaagt
     9301 ttttacagaa gtttatgtaa tgaccaaagg tggaccaata aatagttcga agactctggt
     9361 attttatata tatgaaaaag cttttgagaa cttaaactta ggttatgcag ctgccatggg
     9421 ttttgtatta tttattgtta tatttatact ctcatatttt aactttaaaa tactggggca
     9481 agaataatga tgaggaggga aaacgatgtt taataagtat atagattttt taaaaactct
     9541 tttattatat attttattat ctgttatcgc tattataact atcggcccct ttttatggct
     9601 attatccaca gccctcaaat cgggaggaga gaatatattt tcttatccac ccagttttat
     9661 accaaaaaat cctaccctaa taaattttaa gaaggtatgg aatgctataa acataggtcg
     9721 tttcttttta aatagtacct ttgttgctct tgcgacaatt gttataaata tatttgtctc
     9781 agctttagct gcctatccgt tagctagaat gaaatttaaa ttcaaaaaca taatatttta
     9841 cggaatttta ggaacaatga tggttccttt tcagattctc atgatttcaa tctatatttt
     9901 aagtgtaaga acaggaatga cagatagtta tgccggttta atattaccgg tagcagtaaa
     9961 tgcttttggt atctttttat taagacaggc ttttatcaca ataccggttg atattgaaga
    10021 ggctgctata atagatggtt gtaatagttt tcaaatctgg tataaaatcc ttttaccact
    10081 tattaaacca tcaattgcca ctctggccat ctttaccttt atgatgtcct ggagccgttt
    10141 tatgtggcct ttaattgtaa tcaataatcc cgattactat acacttccgg ttgggatatc
    10201 ctatctttcc ggactgtttt catctaactg gcgtttaatc gcttccggtt cattgatttc
    10261 aattgtaccg attattatat tattcctctt cttacagcgc tattttataa aaggtgctat
    10321 ggcaggggca gtaaagggat gattctagat gaaagacacc ttaacattaa aggaaaatat
    10381 taatataata gaccattatg atgtgattgt acttggcgga ggtaccgctg gttcagtagc
    10441 tggttccgca gctgccaggt tgaatgctaa aactttgatc attgaaagtg aaggagcctt
    10501 aggaggtact tcaactgttg gtcaggtaac accgatgatg cctgtcgaaa tagaaggtaa
    10561 tcctgacagt tctgctttta ataaggaaat taaaaaacgt ttgattgcag aaggttatgg
    10621 agctaaagat tcaaatggta atgatggttg gtttaaccca gaaatgctaa aacacacatt
    10681 agaagatata tatttagaat ataatggtga tattctttat gactgtgaat ttgtgaaacc
    10741 aattgttgaa aatgatacaa taaaaggagt catagtttat aataaaaatg gtttagaagc
    10801 ctatacagca gatcagttta tagatgcaac cggtgatgcc cttgttgcat atagagctga
    10861 tgttccttgt aaggaaggat ctaaagaaga taaataccaa gctgtttctc ttagatttac
    10921 ggtaagtaat attgaattaa agaaattcac aaacttttta tctgaacttg gtcaagatta
    10981 tggacttgaa tatccattag ttgaaacagc tatggtctgg ggggaagggt tcgctcttga
    11041 aaaaatattt ctagaagctc ttgaatctgg tgatctaaaa aaggaagatg gtaaatactt
    11101 tcaggctttt tctatccctg gaatgccaac ctctatgtat tttaattgcc cagagatccc
    11161 aggtaagata aacagccttg atccaaaaga attgaccgag gcaagaatta aaggtagaaa
    11221 aatgatgaaa agattatata atttctttgt agattatcta cccggtttta acaccagctt
    11281 tattgcaagt gaagcctcga ttatcggaat cagagagagc agaagaattg tgggggaata
    11341 tgttttagat agtgaagact atcaaaaaag aagaaaattc ccggatgcga tagcaaattc
    11401 agcctacccg attgatatcc atggagatga acttgaactt aaagaagtta aaaaagggga
    11461 atattatcaa attccttatc gaacattaat tcccaaaaat attacgaact tacttgttac
    11521 cggtaaatca atttcttcaa cctttacaac ccaagcagct attcgaattc agtcaatctg
    11581 ccgcgctaca ggtgaagctg ccggtatagc agctgcaatg gctgtaaaag aaaatattac
    11641 tgttcgagat attgatggca aaaagataaa agaacaaatg ttaaactggg gagcagatat
    11701 ttaaaaagaa aaggtggttt tacttatgtc agataaaata atgtttatac ctctagatga
    11761 acgcccatgt aattataaat atcccgaact aatttataaa atcacattag acaatttaat
    11821 aaaacctcca ctttcaattc taggcaataa aaaagagccg gttgctgtag ataagttaca
    11881 gaaatggata cttaataatt tagaaggaat atcacatcta attctttctg tagacatgtt
    11941 agtttatggg gggataatac cttcgagaat ccatcagctg gacgaaaaag aaatttttag
    12001 tcgcttaaag tttattgaaa aaattaaaga tatcaatcct gatatgaaga tctttgccta
    12061 taatttaata actagagtcc cctcctataa tagtgatgat gaagagcctg attactataa
    12121 atatcacggt gctgatattg caaaatatgg atattattct gatttaataa aacaaaaatt
    12181 agcagaaaaa aaacaaaaag aggaatttga agaaattaaa agtaatattc ccgataaagt
    12241 aataaatgac tatttaaaca gaagagagaa aaaccatgcc ttaaataaaa aggttattga
    12301 ctatgttgaa aataatctga ttgatttttt aatatttccc atggatgata attcagagta
    12361 tgggttttgt gcaaaggaac gtagaaaaat tatcgaaaaa gtctacaaaa aagaattact
    12421 agataaaatc tattcttatc cgggagcgga tgaagttgga acagttttaa ctgcgagaac
    12481 ttttttagat aaaaataatt accgaccaaa agtttatata aaatattctt ctgagaaagg
    12541 tagaaatatt ataccttatt tagaagatag acctttagaa caaaccgtta aataccaaat
    12601 attagcagct aatggtattg tagttgataa cagccaagaa gcagattatg ttcttgtcgt
    12661 taatactcct accaaaaaga tcttaaattc gatcaaaggc tggcagtcaa tattgaatat
    12721 ggatgatatg atagatccta ctcgcaattt aaacgaagta gttgaatcca ttaactatta
    12781 tctatcaaaa gatttaccgg ttgctgtagc agatgtggca gttgtaaatg gttccgacca
    12841 atacctgatg cagttactaa aaaactataa tttaatcact aaattatctg cctatggagc
    12901 ctggaatact tcttctaata caattggctc tgtagttgcc cacgctaata tcttaaatta
    12961 ttatcaaaaa gataacagtc taaccgataa acaaaaagat ttatcaaata gatatctgtt
    13021 tttacgttat ttagaagatt ggggctacca gcatgtagta aggacagaag ttaataacaa
    13081 gctagatgaa tatggcctgg agtattttaa tctaaaagac aaaaccgact ttatttcaaa
    13141 tcttatcaaa gataaattat ataaatttag aaatgaaaat ttagataaat ttagctatga
    13201 ctttactatt gatatgccct ggaatcgaat gtttgaagta gatattaaaa tagagaaata
    13261 agtattttat ggaggtattt aaatctaatg aaaaacttaa aagaaaattc tttaatagtt
    13321 tcttgtcagg ccttagctga tgaaccacta catggttctg aaattatggc taaaatggca
    13381 ttagccgcaa aaagaggtgg agcttctggg gtcagggcta attcacctga agatattaaa
    13441 gctatcaaaa ataaaattga actccccata atcggcctct ggaaaaacaa attaattaac
    13501 tatgaagtat atataacatc gaagtatcaa catgcaaaag cagttttaaa agcaggtgct
    13561 gactatgtgg caatagattg tacagaccga agaagaccgg aaccgctttc tgaaatcttt
    13621 aataaactga gaactaaatt tccagataaa ggtatcgtag ctgatatttc ttcaacagaa
    13681 gattttaaag atatagaaga actagctcct gactatatct ctactactct ctcaggctat
    13741 actgaaaaaa ctcaaaacag aaccaggcct gatatagatt tagtagaaaa attgaatatg
    13801 atctcaaata tacctatcct ggctgaaggt aactacaaaa acggtaagca ggctaaaaaa
    13861 gctttaaagg caggtgccta tgcagttgtg attggagctg caataacaag acctcaggtt
    13921 attactgctc acattataga aaatatgaat taaatttaaa agggatggta tttatgagag
    13981 attttttaaa agaaataaat aataagatga atcataaaat aaaagatatt aaaaattggc
    14041 aggcccggga agctatttac aaggaaaccg ggaaatacaa atttattagc gattgggata
    14101 attttcaatt aggtgatagt tggtctgaag aggaagagat tactactttc tttaaaacta
    14161 aagtttttat cccggaaatc gattttgaaa ataaattaga actaaatatt gaaacaggtg
    14221 gcgaagcggc cctatatatt aacgatgaac ttatccaggg acttgatcgt aaccattcat
    14281 atgttaccat tagtaaagat tacagtagta aagaagtaga actttttata gaagccgccc
    14341 tagatagaat caataaaata tacgtacctt ctcctgatta caagcatgta ttaaataaag
    14401 cagctctttt atcggttaat aagtcagctg aaacttttta ttataatata ctctcattaa
    14461 tagaattaat agaaacctta gaagaaagaa ataaaaaaga tgttttagtt caactgaaag
    14521 acattttctc aactttgaaa gaagattata gaagttatcc gaaatctaaa attgatttta
    14581 atattttaaa taagaaatat agtaaactaa taaataatat tgattacgat acaacctata
    14641 aaattgcctg ttttgggcac gctcatatcg atgtcgcctg gctttggcag tttaaggaaa
    14701 ccgttcgtaa atcagctcga agtttttcta cagcggttaa tttaatggaa gagtaccctg
    14761 aatttaaatt tattcaatct caacctaaac tatatcagta tatgaaagaa tattacccca
    14821 atctttatga aaaaataaaa gacaaaattg ctgataaaaa ctggcaggtc gaaggtggca
    14881 tgtgggttga agccgatact aatcttttaa gtggagaatc tttggtaaga cagtttttat
    14941 atggaaaaaa atttattaga gaagaattca acgtagaatc taaagtgggc tggttaccag
    15001 atgtttttgg ttatacagct tctttacctc aaattatgaa aaaatccgaa gtagattatt
    15061 tcatgacatc aaaaatatca tggaatgata ccaataattt tccttattca atcttccgct
    15121 gggaaggaat cgatggtaca gatgtcttaa cacatgtacc tcgtgtatta ttaccttata
    15181 cctataatgg tgaagtctat gccgataaaa tattaaaggt tagagataac tatgaaatgc
    15241 agaaagaaac cgaaatagaa gaatttaaat atggtgattc atcaaattac tttaaacagg
    15301 atagagtaat tcccgatgac caattaattt atatctatgg ctatggtgac ggtggtggag
    15361 gagttacccc tagtttcata aataagatta agcgatttga taaattacca tatatgccta
    15421 aacttgaatt tgcacaaccc cgtgaatact ttgagaattt agaaaaggac atgcagataa
    15481 ataataggga atacccaacc tggaagggtg aattatacct ggaatatcat agagggacct
    15541 ataccactca agcggagata aaacgtcaaa ataggtacct agaggacaat ataagagagg
    15601 ctgaaatact taatacaatc acagaacggt atccccagaa caaatttgaa aatttatgga
    15661 aaaaaatcaa tttaaaccag ttccacgata ttattccagg ttcttcaatc aaagaagttt
    15721 atgaagacgt caatcaaatc tatgcagaag ttactgatga aataaacaac attttaaaaa
    15781 ataacctaag ttcattgggt aataatattg aaattaaatc aaaagataat agtaaatatt
    15841 atttaatttg gaatggactt gattataaaa gaagtagtta tataaagttt agtacggaag
    15901 aatttgataa ggatattgaa gtttttgatt atataaccgg aaaaaaactt aagaataata
    15961 gcaaagaaga tctgaaggaa aaaaaactat ttgaaatata tatcgaagat gtacctccat
    16021 tcggctacag gataatagaa ataaaagaaa ataaagaacc aaataatgac atgacttcta
    16081 aattaaataa aaataacatt gataaaactt tagaaaatag ttattataaa attaaattca
    16141 aggaaggcct aattacctca ctttttgata aaagaagaga agtggagtta attaaagagg
    16201 gtaaagtagg taatgaatta cagtttttcg ttgataaacc ggataaatac gaggcctggg
    16261 aacaggatgc agacttcgaa gaacatagaa tatatgatga attagaatta gatagttttg
    16321 aaattaaaga atataatacc caaaaagtgg cacaactgaa atggaacttc cgcaaatcag
    16381 tatttaaaca aaatattctt atttatgata accttgatcg aatcgatatc aaaaacaaag
    16441 tagcctggcg agacagagaa atactagtca aagctgcttt cccacttaat attctatcta
    16501 atgaagcccg atatgaaata gcctatggta atattacaag acccacaaca aataatacta
    16561 gttatgaaaa agctcaattt gaagttccgg gacatcgttg ggccgactta tctgaaccgg
    16621 gtttgggttt tagcataata aacgattgta aatatggtta tgatattaag ggtagtttaa
    16681 tgcggttgac tttgttaaag tcaccgaatt atcctgacga aactgccgat tacggtatcc
    16741 atgagtttac ttattctctt tatactcatg acggtattta ttacaacagc aacttactaa
    16801 aacatgctga tgatttaaat agaccatttt taacaaaaaa cattgaaata aataatgatg
    16861 ttaaagataa tagtaatttg tcatatagtt ttgctaaaat cattaaaggt aactccaggc
    16921 ttgaagtaat caaaagatca gaacaaaatc cagataaagt tattttgaga tttttcgaac
    16981 catatggtgg aaaagataaa gttgaagtag aattaactaa agaaataaaa aatgtagaaa
    17041 ctgttaatct aattgaaaac aaaattaatg ataaagtaaa atacgaaaaa aacaatttaa
    17101 attttgaaat ggatcctttt gaaataaaaa caatcaaatt agaacttata gataattaat
    17161 tagttaaggg tgataagatg agggtattaa ttgaaaaaga ttataatgaa atgagtaaaa
    17221 aagctgccta tttagttaaa aaacaaataa atgataaacc cgatagcgta ttaggactac
    17281 cgactggaag tactccttta aagatgtata aggtactaat agagatgtat agaaggggag
    17341 aggtagactt ttcagaagtt actacattta atctagatga atattatggc ttaccatttg
    17401 atcattcgca aagctaccat tactatatgt ggaataactt ttttgaccat ataaacatta
    17461 aagatgagaa tgtacatatt ttagatggta tgacggaaaa tatagatcaa gaacgtattg
    17521 aatacgaaac caaaataaaa aatgcaggtg gaatcaatct acaggtctta ggtattggcc
    17581 caaacggaca tatcggattt aatgaacctt ctgaaaccct aaatgataaa acacatctcg
    17641 ttactttgac tgaagaaact agagaatcaa atagtcgctt ttttgatagt aaagatgaag
    17701 tgcctaaaaa ggctttaact atgggaattt ctacaatact taaagcagat aaaatattaa
    17761 taatggcttc cggagaaggt aaagcctctg caatcaaaaa gactctaagt ggacaaatta
    17821 atacctgtac accttcaacc ctcctccaga ctcattcaaa tgtgaccgta gtaatagatc
    17881 agggtgcagc tatttacttg taaaataaat ctaactttaa aaatgaattt aattgcttat
    17941 aataaaaaag ctaactggta attccagtta gtttttttat ttaagttcct ttttatttta
    18001 ataaagatag ttaattaaat aagttatttt gttaatatac ttcccatatt aaaaattttc
    18061 atgttattta tggttccaac acaaaaatca tataatattt tccaattata gataaaaata
    18121 actataatgt ttctcttaaa aaagtctgat tttgttcaat aaaaacacct ataatctatg
    18181 ttatatcaat ggttatatga tataatataa gtagattata taaaaaacga ggtgttttta
    18241 tgtgggctga aaataatttc caacagttgt cttttgacga tatattattg aatatgccta
    18301 aatatcttaa aaatttcctt aaagatagct gggctgatgt ttttcaagaa ataatattcc
    18361 ctgcaattga tgaaaaacga ttttctgttc tatacagtga taaaccttca agacctaatt
    18421 cccctgttaa tgtaattata gggtccttga tcttaaaaga gatttttcaa ctttctgata
    18481 aagaacttct aggtgctatt tattttgatg atcgttttca gtatgcacta cgtttaacta
    18541 gtgaagaaaa accacctatt tctataaata ccttcagcaa ttttagaaga cgggtctatg
    18601 aattcgaaca agaaaccaat cgtgacctca taaaagagga agtaaaaagt atttctaatt
    18661 taattgcaaa aaaattaaag attgataaca aaaaagttag agttgattct tttatgtttg
    18721 cttcttcttg taggaactta tcccgattgg aattaatata tactgtcaat agacaaatgg
    18781 caaaaaagct aaaacaagaa gatgaagata ttataccgca tggatttcat caatatttaa
    18841 aagatggtca taaaaaaaat actatttata caacacacga taatgaagca gagtctaaat
    18901 tagagttttt attaaaacac tctaaaacct tttatgatct tattttagaa agcagtttag
    18961 atatttataa atcacaagaa tttaaaaatt taaagaggct acttaataaa caaactaaaa
    19021 gcaatgataa agatgatgat gataataact acacaccaaa agaaaatggt gagattaaac
    19081 ctgacagtct ttaaaaccct tttgatcctg atgccactta ccggtttaaa tatgatgata
    19141 atatcgggta cactggtaat gtagtagaag cttttgatgg tgaaaacaat attattaaaa
    19201 attatgatct aaagcccaat acatacagtg atcaaaaatt tagtagggac actattaaaa
    19261 cactttctaa aacaacagaa agaaaagaac ctttaaagat gttaatagac ggaacatatt
    19321 tttctataga actagctaaa
//
LOCUS       DATGCJ010000108         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_280511, whole genome shotgun sequence.
ACCESSION   DATGCJ010000108 DATGCJ010000000
VERSION     DATGCJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280511"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..170
                     /locus_tag="VKN64_05335"
     CDS             9..170
                     /locus_tag="VKN64_05335"
                     /inference="COORDINATES: protein motif:HMM:NF018571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59699.1"
                     /translation="MEGNDSVSSNRVVKFDNKKKISDTDIGNKLKGKVDNLMELLKAY
                     NKGIIKEKY"
     gene            complement(259..456)
                     /locus_tag="VKN64_05340"
     CDS             complement(259..456)
                     /locus_tag="VKN64_05340"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMA59700.1"
                     /translation="MKKNVGKSDKIIRYILGIVIIAIGIMYNSWWGAIGIIPIFTAAS
                     GWCPLYSIINTTTKKENKKIG"
     gene            complement(673..748)
                     /locus_tag="VKN64_05345"
     tRNA            complement(673..748)
                     /locus_tag="VKN64_05345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Thr,seq:tgt)
     gene            937..1233
                     /locus_tag="VKN64_05350"
     CDS             937..1233
                     /locus_tag="VKN64_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HMA59701.1"
                     /translation="MANKYRSNKIKKEHTIIEDILPLLESISIYPEIKSIIPGRINQR
                     SSSSGQKAYLQIKYNTNSGLKLLAKNSSSVQEVFVVTKDTDKTMTRLKKLEFTN"
     gene            1280..1356
                     /locus_tag="VKN64_05355"
     tRNA            1280..1356
                     /locus_tag="VKN64_05355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1314..1316,aa:Pro,seq:ggg)
     gene            1432..2763
                     /gene="rimO"
                     /locus_tag="VKN64_05360"
     CDS             1432..2763
                     /gene="rimO"
                     /locus_tag="VKN64_05360"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMA59702.1"
                     /translation="MIKVSFVTLGCPKNEVDTDYMMGITFNDDKYKYEEQPKEADVIV
                     INTCGFIEDAKEESIDTILQAAEYKKDGLIDALIVTGCLTQRYKTELMEEIKEIDAVL
                     GTGTFDQIKETIDDVLNNKKRYNRIDKPVFDFNQNLERFNKQNHYSYVKIAEGCNNNC
                     SYCSIPFIRGPLNSRKMEDIIKEIDILVENGAKEIILTAQDTTEYGKDLYGERKLVDL
                     LKEVVKIKDLKWVRLLYSYPEHISQELIDVIKNNDKICNYLDIPVQHSSNKIRKQMRR
                     EGTKEELLNVINDVRKNINDVALRTSLIVGFPGETEDDFNNLLSFIKEVKFDRLGVFQ
                     YSREEGTKAALMANQIDKEIKQKRYNKIMEIQQNISYNKNKELIDRGLDVIIDEEIDD
                     EFIGRTVYDAPEIDNTVYVKGKNISIGDIVKVKITNAFEYDLMGEKIYEST"
     gene            2750..3304
                     /gene="pgsA"
                     /locus_tag="VKN64_05365"
     CDS             2750..3304
                     /gene="pgsA"
                     /locus_tag="VKN64_05365"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HMA59703.1"
                     /translation="MNLPNKLTLLRIILVPIFIVFLLMRKNIPGAEVFSLAVFILAAI
                     TDSLDGYIARKQQIVTKFGKIVDPVADKLLISGALIAFVALDEISLWAAILIIGREFA
                     VTGLRVIAAKDGVIISASIWGKIKTTIQITAVIAVIADPKIITLPYNIPNILIWVAVL
                     ITLYSGYKYFQAVNIDFFVEEDHH"
     gene            3309..3767
                     /locus_tag="VKN64_05370"
     CDS             3309..3767
                     /locus_tag="VKN64_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HMA59704.1"
                     /translation="MKAIKNKIIEVVASCFYIGYIPGAPGTYGSLFALLLISQFNVLT
                     KNISVIAFIIIGLLSSTLMEKHTGKKDDQRIVIDEFVGMLITFYFIEPNFTYLIVGFI
                     LFRLYDIYKPYPIKKIQNLPSGWGIMLDDILAGVYARIVLHIFVVLQIGF"
     gene            3822..5048
                     /locus_tag="VKN64_05375"
     CDS             3822..5048
                     /locus_tag="VKN64_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HMA59705.1"
                     /translation="MNVEIISIGDELLDGSVQERNANFISNKLKKYNIIPHHICIVGD
                     NIIKILNLIKTAKKRSDIIFITGGLGPTGDDITRNAVSKAFDIELEFHEDIKKRIEKR
                     LREFHVDHTDNNNQQAMIPKGAKVLPNERGTAPGFIINKDNTTCICMPGVPDEMKPMF
                     KKQVISFLDLANKEIYKEELFFKGIGEAQLEDKIYSSVKVPEDIYLSFVVKKTHILVK
                     VNGKDKKLVQDTTDKIKDKLGQNIFKDLNLIETIYNIMKNNKLTLSTAESCTGGLLGN
                     RITNLSGSSIYYLGGIVAYSNELKINMLNIDKEIIEIKGAVSSETAESMADNIKEITG
                     SDYGIGITGIAGPTGGTKAKPVGLVYIGVSGKNETKVKEYQLKGKRKQIKYLSSEFAL
                     KDLKYLLYREGYHEKE"
     gene            5035..>6146
                     /locus_tag="VKN64_05380"
     CDS             5035..>6146
                     /locus_tag="VKN64_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA59706.1"
                     /translation="MKKNKLLVLMLILTFVVFSSIAVNAQSEQLDLLSRSIKNYHLSN
                     YQEALDLNTQINVDELTTSAKIDTLYYRTLIKLALGNISTAKEDLSQLNEMGYDFGMI
                     HYELARIYMNFYNNFDNAYYQTALEEFKKARALGINSPQFHRDYAMAFMGINNKQRAV
                     QQLEMAIIENGSVDVLLNIANLHKELGNYDKAADYYNQVIEMDNKNVQAYGELGDVLI
                     RQEKYAEAAEVLQNGVELNPDSLVVNFQLGKAYYYTENYEQGEQYLTKVIELKQNYYR
                     AHYYLGKLYEKQENYQNALYYLKEAVKYNPNFADAYISLGDINLKQGKNYQAIANYST
                     AIEQNPEYPPGHFHLALAYIESDMKEAAISELRKTL"
BASE COUNT         2280 a          936 c         1106 g         1824 t
ORIGIN      
        1 ttgcggtaat ggaaggtaat gattccgttt catcaaatag agtcgtaaag ttcgataata
       61 aaaagaagat ctctgataca gatataggaa ataaattaaa aggtaaagtt gataatttaa
      121 tggaactact caaagcatat aacaagggga ttattaaaga aaaatattaa aaaatcattt
      181 gtaaaagtta atattaaaaa aagttaatat taaaataagt taatgggcca gtttataaac
      241 tgacccattt cttatatact aaccaatttt tttattttcc ttttttgtag ttgtattaat
      301 tatagaatag agtggacacc aacccgaagc agctgtaaag ataggtataa ttccgattgc
      361 tccccaccaa ctattgtaca taataccaat tgcgataatc acaattccca aaatatacct
      421 gattatctta tcggacttgc caacgttttt cttcatgata agttccctcc tccttacttt
      481 caactatcta tattttacca gataattaaa tatagataag taaggtactt aactagacca
      541 aaaatattta cacaattatg tattaacaat ataaacgttt aacaacaaaa aaacccgggt
      601 agaacccgag tttataatat aatgatatta ttcattatta taataaaata aaaaattaac
      661 caaaaataaa aatggagctg atgaccggac ttgaaccggt agcctgctga ttacaagtca
      721 gctgctctac caattgagct acatcagctt gaataatttt tatgctgttt tcaatttcat
      781 acagcaatta ttataactta gaattgttgt caggtcaagg gttaaatctt aattttttaa
      841 tattttccca aaattttttc tctaactata ttatacacca tatttgtttg taatatcacc
      901 cctacaatga tataattttt tcaggaagtg ataaagatgg ccaataaata tagaagcaat
      961 aagatcaaaa aagaacatac aattatagaa gatatactac cacttttaga aagtatatca
     1021 atatatccag aaataaagag tataattccg ggcagaatta accagagaag cagtagtagt
     1081 ggtcaaaaag catatttgca gatcaagtat aatacaaata gcggtttaaa actgcttgct
     1141 aaaaacagct cttctgtaca ggaagtattt gtggtaacta aagatactga taaaactatg
     1201 acaaggctta aaaaattaga attcaccaac taaatccttg actttttagt aattgtggga
     1261 tataattttt aacagtttgc gggttgtggc gcagcttggt agcgcgcacg cctggggggc
     1321 gtgaggtccc gggttcaaat cccggcaacc cgaccatttt atttatatga attttaacaa
     1381 gcccggcctg ggcttttttt atatttaaaa gaagaaaaag ggtgtatatt aatgataaaa
     1441 gttagttttg taactttagg ttgtcccaaa aatgaagttg atacagatta tatgatgggc
     1501 ataaccttta atgatgataa atataaatac gaagagcagc cgaaagaagc agatgtaata
     1561 gtaattaata cctgcggctt tatcgaggat gccaaagaag agtcaatcga caccatttta
     1621 caggcggctg aatacaaaaa ggatggatta atagatgctt taatagtaac gggctgtcta
     1681 acccaacggt ataaaactga gttgatggaa gagattaaag aaattgatgc ggtcttagga
     1741 actggtacat ttgatcaaat aaaagagacc attgatgatg tattaaataa taaaaaaagg
     1801 tacaatagaa ttgataaacc ggtttttgac tttaatcaaa acttagaaag atttaataaa
     1861 caaaaccatt attcctatgt taagatcgcg gaaggctgta ataataactg cagctactgt
     1921 agcatccctt ttattagggg tcctttaaac agccgcaaaa tggaagatat aataaaagaa
     1981 atagatatat tagtagaaaa cggggctaaa gaaattattt taaccgccca ggatacaacc
     2041 gaatatggaa aagaccttta cggggaacgt aaactcgttg acttattaaa agaagttgtt
     2101 aaaataaaag atcttaaatg ggtcaggtta ctttacagtt atcccgaaca tatctcacaa
     2161 gaattaatag atgttatcaa aaacaatgac aagatatgca actatttaga tatcccggtc
     2221 cagcattctt ccaataagat tagaaaacag atgaggagag aaggaactaa agaagagttg
     2281 cttaatgtaa ttaatgatgt tagaaaaaat ataaatgatg ttgccttaag gacctcactt
     2341 attgtcggtt ttcccggtga aacagaagat gactttaaca acctgctttc ctttatcaag
     2401 gaagttaaat tcgacagatt aggggttttt caatattcga gagaagaagg aactaaagcc
     2461 gctttaatgg ctaatcaaat agataaagag ataaaacaaa aaagatacaa taaaataatg
     2521 gaaatacaac aaaatatatc atataataag aacaaagaat taatagacag aggactggat
     2581 gttataatcg atgaagaaat tgacgatgaa tttataggca gaactgtcta tgatgcaccc
     2641 gaaattgata acactgtcta tgttaaaggt aaaaatataa gtataggaga tattgttaag
     2701 gtcaaaatca cgaatgcttt tgaatatgac ttaatgggag agaaaatata tgaatctacc
     2761 taataaatta acccttttaa gaattatttt agtccccatc tttatagttt ttttactgat
     2821 gagaaaaaac ataccggggg cggaggtatt ttctttggct gtatttatcc tggcagccat
     2881 taccgacagt ctggatggat atatcgcaag aaaacagcag attgtaacga agttcggcaa
     2941 gatcgtagac ccggttgctg ataagctatt aatctcaggc gctttaattg cctttgttgc
     3001 cctagatgaa atttctcttt gggctgctat attaattata ggccgcgaat ttgctgtcac
     3061 cggtttaaga gtaatcgcag ctaaagatgg tgtaataatt tcggccagta tctggggtaa
     3121 aataaaaacc actatccaaa ttacagccgt tatagctgta attgcagatc ctaaaattat
     3181 taccttgccc tacaatatac ctaatatctt aatctgggta gccgtattaa tcaccttata
     3241 ttccggctat aaatacttcc aggcagtcaa cattgatttt ttcgttgagg aggatcacca
     3301 ctaaaacaat gaaagctatt aaaaataaga taatagaagt agttgcaagt tgtttttaca
     3361 taggatatat accgggtgca cccggaactt atggttcctt attcgccctg cttttaatca
     3421 gtcagtttaa tgtcttaact aaaaatattt ctgttattgc ctttataata attggtctgc
     3481 tttcatctac ccttatggaa aaacatacgg gcaaaaagga cgaccaaaga attgttatcg
     3541 atgaatttgt gggcatgtta ataacctttt attttattga acccaatttt acttatttaa
     3601 tagtcggctt catcttattt agactctatg atatatataa accatatcca attaaaaaaa
     3661 tacagaactt accctcaggc tggggaatta tgctggatga tattctggcc ggtgtctatg
     3721 ctagaatagt cttacatata tttgttgtcc ttcaaatcgg tttttaaaaa ccgatttttt
     3781 ttattaatta aatatgatat aatttattcg aggtgaatac attgaatgta gaaattataa
     3841 gtatcgggga tgaacttctg gacggatctg tccaggaacg taacgccaac tttatcagta
     3901 ataagttaaa aaaatataat ataatacctc accatatatg tatagtggga gataacataa
     3961 tcaaaatatt aaatctaata aaaacagcaa agaagaggtc tgatattatc ttcatcacag
     4021 gtggtttagg gcccaccggt gatgatatta ccagaaatgc ggttagtaag gcctttgata
     4081 tagaattaga atttcatgaa gatattaaaa agaggatcga aaaaagatta agagaatttc
     4141 atgtagacca tacggataat aataaccaac aggccatgat tcctaagggt gcaaaagtat
     4201 tacccaatga gcgaggaacg gcccccggtt tcataatcaa taaggataat acaacctgta
     4261 tctgtatgcc gggtgtgccc gatgagatga aaccgatgtt taaaaaacag gtaatatcat
     4321 ttcttgattt agcaaataag gaaatatata aagaagaatt gttctttaaa ggaatagggg
     4381 aagcccaact agaagataaa atatatagct cagttaaagt ccctgaagat atctatctca
     4441 gttttgttgt aaagaaaact catattttag taaaagtgaa tggtaaagat aaaaaattag
     4501 tccaagatac tactgataaa attaaggata agttagggca aaatatcttt aaagatttaa
     4561 acttaattga aaccatctat aatatcatga aaaacaataa attaacctta agtacagccg
     4621 aatcctgtac tggcggcctg cttggtaata ggatcaccaa tttatccggc agttcgatct
     4681 attatctggg cggaattgta gcttacagta atgagttaaa aatcaatatg cttaatatag
     4741 ataaagaaat tatcgaaatt aaaggtgcgg taagctctga aactgctgaa agtatggcgg
     4801 ataatattaa agaaattaca ggctctgact atggtatagg tattacgggt atagcgggcc
     4861 caaccggtgg gacaaaagcc aaaccggtag gacttgtcta tataggagta agtgggaaaa
     4921 atgaaactaa agtcaaagag taccaattga aaggaaaaag aaaacaaatc aagtatttaa
     4981 gtagtgagtt tgccttgaaa gatttaaaat atctgttata tagggagggt tatcatgaaa
     5041 aagaataaac ttttagtttt gatgttaatt ttaacttttg tcgtgttttc tagtattgca
     5101 gtcaacgctc agtcagaaca gttggattta ctaagtagga gtataaaaaa ttaccatctc
     5161 agtaactacc aagaagccct ggatttaaat acccagatca atgtagatga actaaccact
     5221 tcagctaaaa tagacactct ttattaccgg actttaatca agcttgcttt aggaaatatc
     5281 tccaccgcaa aagaagattt aagtcagctt aatgagatgg gttatgattt tggaatgatc
     5341 cactatgaat tagccagaat atatatgaac ttttataata actttgataa tgcctattat
     5401 caaactgctc tagaggaatt taaaaaggca agggcactcg gtataaattc tcctcagttt
     5461 caccgggact atgcgatggc ctttatgggt atcaacaata aacaaagggc cgtacagcag
     5521 ttagaaatgg caattatcga aaatggatca gttgatgttt tacttaatat agctaacctg
     5581 cataaagaat tgggtaatta tgataaagca gccgattact ataaccaggt tattgagatg
     5641 gacaataaga atgtacaagc ctatggtgaa ttaggagatg tcttaatcag acaggaaaaa
     5701 tatgctgaag cagccgaggt gctgcaaaat ggggttgaac taaatcccga ttctctcgtt
     5761 gttaacttcc aattaggtaa agcatattat tatacagaaa actatgagca gggtgaacag
     5821 tatttaacca aggttatcga acttaaacaa aactattata gagcccacta ttatttaggt
     5881 aagctatatg aaaaacaaga aaattatcaa aatgctttat attatctcaa ggaagcagtc
     5941 aagtataatc ccaatttcgc ggatgcctat atctcgttag gagatataaa cttaaaacag
     6001 ggtaaaaatt atcaggccat agccaactat tctacagcga ttgaacaaaa ccctgagtac
     6061 ccgcccggac actttcatct cgctttagcc tatatagaat ctgatatgaa agaggccgcg
     6121 atttcagaat taagaaaaac ccttca
//
LOCUS       DATGCJ010000109         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_505372, whole genome shotgun sequence.
ACCESSION   DATGCJ010000109 DATGCJ010000000
VERSION     DATGCJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505372"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VKN64_05385"
     CDS             <1..769
                     /locus_tag="VKN64_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMA59707.1"
                     /translation="VGKSTVTANLAIALQKEGKQVGIIDADVHGFSIPRIIGLEEQVK
                     VEQESELIHPAEKMGVKVISMGSFVHEKNPVIWRSPLLLGALQQFFKDVLWGDLDYLL
                     LDLPPGTGDMSLNIMQKLPHARTMIVTTPQITATNVAGRVGHMAEKMDSDVLGIVENM
                     AYYECSECGNKEYIFGKGGGEELAELLGVDLLGQLPLMTHVREGGDKGNPVVDEDSND
                     DISQEFLKIAQEVIKRKKEFDPSLKPMNLEGGPGGNQ"
     gene            complement(844..1653)
                     /locus_tag="VKN64_05390"
     CDS             complement(844..1653)
                     /locus_tag="VKN64_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMA59708.1"
                     /translation="MLEWLAGFSPITQALFATLFTWFLTALGAALVFFFTDVKRKILD
                     AMLGFAAGVMIAASYWSLLAPAIEMAENQGYIPWVPAVFGFLLGGAFLYLVDKTLPHL
                     HLGLDVEEAEGIHTTWQRSVLLVLAITLHNIPEGLAVGVAFGAASFNLPSASLAGATA
                     LALGIGIQNFPEGTAVSVPLKREGFSAVKSFNYGQLSGVVEPIAGIIGAWAVLSMRPL
                     LPYALSFAAGAMIYVVIEELIPESQSEGTDIATLGAMVGFSIMMLLDVALG"
     gene            1794..3080
                     /locus_tag="VKN64_05395"
     CDS             1794..3080
                     /locus_tag="VKN64_05395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HMA59709.1"
                     /translation="MKLLQEFINQEIGLANGCTEPAAVAYSVARAADFLEGDLEKIVV
                     EMDGFVYKNGMRTGIPGTNRYGGNQLAAALGFFIENSADKKLTLLNGLKEETLQKADS
                     IIDKIELKVIKGQRDLLIKTKLIGDNVVKSKIEDDHTNLTEINVNGETIYKNTNQASE
                     DNIEFNLKNEIESMNVNETIKLVERQYDSSMEEYLKQGLKINQKLAEAGMKDEGCGVG
                     RTYKKAAADDFVRSSAAEIAAAVDARMGGAQLPALTSSGSGNQGIFITLGTYLSGQNM
                     EKEETEIFKATLIAHLIAYYIKTKMGKLSSLCGLFTAAAPGMLAGLLYLVKKNEMIED
                     GINNLFSDSGGILCDGAKTSCAFKAISAYELAQRHFKFLDSGIECPLPTGYVNSDLKK
                     TLEHQYALVNPGGVSVNDALIETIKANFAKKGVDKK"
     gene            3077..4423
                     /locus_tag="VKN64_05400"
     CDS             3077..4423
                     /locus_tag="VKN64_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA59710.1"
                     /translation="MKIAVVGGVAAGTSAAAKASRTAEDAEIVLFEKGEDISYAGCGL
                     PYYISEVTKSRGKVVINTPEEFEEKYGVDVRIKHEVIDIDKEKKVLTYKDLENDTKGE
                     YQYDKLIITTGAKPIIPPIDGIDLENIQPLRSVKDADQIKEKMKDAEKVTIIGAGLIG
                     LEMAESFSELGKEVTVVELMNKILPQFSQDITKYVDNHLREQGVNLVLGDGVNSFKGD
                     EKVERIITSSGQKIDTDFVLLSLGIKPEVNLAKQAGIGIGKTGAIEVNERMETNVEDV
                     YAAGDCAETINLLTDQRVWIPMGSTANKQGRVAGENAAGGDNHHLGVFQTAITKIFDY
                     TVAKTGIDEKEAAENGYDPVVVTITGANHAHYFPNFKKMKIRGVFDKKSHRIIGAQIE
                     GESKVDKRIDVISTAIYSELRAEDLFQIDLAYAPPYSTPKDPVAVLGMVAEKKLNQ"
     gene            complement(4488..5012)
                     /locus_tag="VKN64_05405"
     CDS             complement(4488..5012)
                     /locus_tag="VKN64_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMA59711.1"
                     /translation="MKLMKNKGLITVIALLLLTAGTLFYVNEIQAASDDVKVGAQVGM
                     KAPDFTLEDMSGEKISLYDLQGQKVFLNFWASWCPPCKAEMPAIQKLYKNNENIKVLT
                     VNIQEGKDKVFDYLMKNNFSFTTLLDKSGTVASSKYLVRGIPTTFILDKDSIIQARHS
                     GALTYEQMQDLVSK"
     gene            complement(5025..>5403)
                     /locus_tag="VKN64_05410"
     CDS             complement(5025..>5403)
                     /locus_tag="VKN64_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMA59712.1"
                     /translation="GIIKLKWLYYEKKLNITKRSNKYVQSFILGVGIALAWTPCIGPI
                     LSSILILAGNSQNLMTGGFLLFIYSFGFALPFLITALAAKKILPAIKKMNRYLNYINM
                     VSGALIIILGILIFTNNLNIIGF"
BASE COUNT         1879 a          921 c         1128 g         1475 t
ORIGIN      
        1 agtcggtaaa tctactgtca ccgccaattt agcaattgcc ctgcaaaaag aaggtaagca
       61 ggttggaatt atagatgctg atgtacacgg atttagtatt ccgcgtatca tcgggttaga
      121 agaacaggta aaagtggaac aagaatctga attaattcat cccgctgaaa agatgggagt
      181 taaagttatc tcaatgggtt cttttgtcca tgagaaaaat cccgtgattt ggaggagccc
      241 cttactctta ggagccctac agcagttctt taaagatgtg ctctggggtg atttagatta
      301 cctcttactg gacttacctc ctggtactgg tgacatgagc ttaaatatca tgcagaagtt
      361 acctcacgcc agaactatga ttgtaacaac tcctcaaata accgctacca atgtggccgg
      421 acgtgttggt catatggcag aaaagatgga ttcagacgtt ttaggtattg tagaaaatat
      481 ggcctactat gaatgctcag aatgtggtaa taaggaatat atctttggta aaggtggcgg
      541 ggaagaactc gctgaattac tgggtgtaga cctcttagga caattaccgc tcatgacaca
      601 cgtaagagaa ggcggagata aaggtaatcc ggttgtagat gaagatagta atgatgatat
      661 aagtcaggaa tttttaaaga tagcccagga agttatcaaa aggaaaaaag aatttgatcc
      721 cagcttaaaa ccgatgaacc tggaaggtgg acccggtggt aatcagtaat ttcggaaaaa
      781 aataagaagc tagagcacct ggattcgcca ggtgcttttt ctttggtgta tttgttatgg
      841 tctttaacct aaagcaacat ctaaaagcat cattatcgaa aaaccaacca tggcccctaa
      901 agttgcaata tcagtacctt cagattgaga ttctgggatt aattcttcaa ttacaacata
      961 aatcatggca ccggctgcaa aggataatgc ataaggcagt aggggacgca tacttaacac
     1021 tgcccaggcg ccaatgatac cggcaattgg ttcaacaaca ccggacaact gtccataatt
     1081 gaagcttttt acggccgaga atccttctct ttttaggggc acagatactg cagttccctc
     1141 tggaaagttt tgaataccga tacctaaagc taaggcagtt gctccagcca gagaggctga
     1201 ggggagatta aaagaggcgg ccccaaaagc cactcccaca gctaaacctt ctggaatatt
     1261 atggagtgtg atagctagaa ctaataatac actccgctgc caggtagtat ggataccttc
     1321 tgcttcctcc acatccaagc ctaaatggag atggggaagg gttttatcta ctagatacaa
     1381 gaaggctcct cctaatagaa aaccaaatac ggccggaacc cagggaatat atccttgatt
     1441 ttctgccatt tcgatggcag gtgctaaaag tgaccaataa ctagctgcaa tcattacccc
     1501 ggctgcaaaa cccagcattg catcaagtat ttttctttta acatcggtaa aaaagaacac
     1561 caaggctgcc cctagggcag ttagaaacca ggtgaaaagg gtggcaaaaa gagcttgtgt
     1621 tataggacta aaaccagcta accattctaa catttggata aacctcccag ttttttatta
     1681 tatatattaa taacacattt acattctatg tatataagat taaaggattt ttatatttta
     1741 atcaagaata aagataatta agaaaaaaag aatataggag ggcggaaaat cagatgaaat
     1801 tactgcaaga gtttatcaat caggaaatag gtctggctaa cggctgcaca gaaccggcag
     1861 cagtagccta ttcagtagct agagcagcag attttttaga aggtgatttg gaaaaaatcg
     1921 tagtagagat ggatggtttt gtatataaaa acggtatgag aaccggtata cctggcacta
     1981 atagatacgg tggtaatcaa ctagcagcag ccctcggatt ttttatagag aactcagccg
     2041 ataaaaaact cactttatta aacggcttaa aagaagagac tttacaaaag gcagactcta
     2101 ttattgataa aatagaatta aaagtaatca aaggtcaaag agacttactt attaaaacaa
     2161 aacttattgg cgataatgta gtaaaaagta aaatagaaga cgaccatact aatttaactg
     2221 agataaatgt taatggagaa acaatatata aaaataccaa tcaggctagt gaagataata
     2281 ttgaattcaa tctaaaaaat gaaatagaaa gtatgaatgt aaatgaaacg ataaaattag
     2341 tagaaagaca gtatgatagt tcaatggaag agtatttaaa acagggctta aagataaatc
     2401 aaaaactggc ggaagccggt atgaaagacg aaggttgtgg agtaggcaga acatataaaa
     2461 aagctgcagc cgatgatttt gtgagaagtt cagcggcaga aatagcagcg gcagttgacg
     2521 ccaggatggg cggagctcag ctgcctgctt taacttcatc cggcagcggt aaccagggga
     2581 tatttatcac actcggtacc tatctttccg gtcagaatat ggaaaaggaa gaaacggaaa
     2641 tctttaaagc aacccttatc gcccacttaa tagcatatta tataaaaaca aaaatgggta
     2701 agttatcttc tttatgcgga ctttttacag ctgctgcccc gggaatgtta gctggtttat
     2761 tgtatcttgt taaaaaaaat gaaatgatag aagacggtat aaataattta tttagcgatt
     2821 cggggggcat tctctgtgat ggagctaaga ccagttgtgc ctttaaagcg atcagtgctt
     2881 atgaattagc acagcgacat tttaaatttt tagatagtgg tattgaatgt ccacttccca
     2941 ctggatatgt taacagtgat cttaaaaaga ccttagaaca tcaatatgca cttgtaaatc
     3001 cggggggagt ttcggtaaat gatgctttaa ttgagacaat taaggctaat tttgctaaaa
     3061 aaggagttga taaaaaatga aaatagcagt tgttggaggt gtagcggcag gaacaagtgc
     3121 tgcagcaaaa gcaagtagga cggctgaaga tgcagaaata gttctattcg aaaaagggga
     3181 agatatttct tatgcaggtt gtggactgcc ctactatatt tctgaagtaa ctaaatccag
     3241 aggtaaggta gtaataaata ctcccgaaga atttgaagaa aaatatggag tagatgtacg
     3301 aataaaacat gaagttattg atatcgataa agagaaaaaa gttttaactt ataaagacct
     3361 agaaaatgat actaaaggtg aatatcaata tgataaattg attattacca caggtgccaa
     3421 acccataatt ccaccgattg atggtataga tttagaaaat attcagccgc tccgttcagt
     3481 aaaagatgcc gatcagatca aagaaaaaat gaaagatgca gagaaagtaa caattatcgg
     3541 agccggtcta attggtcttg aaatggctga atctttttca gaattaggta aagaagtcac
     3601 tgttgtggaa ctaatgaata aaattttacc tcagtttagt caagatataa ccaaatatgt
     3661 agacaatcac ttaagagaac agggagtaaa tcttgttcta ggtgatggag taaactcttt
     3721 taaaggtgat gaaaaagttg aacggataat cacaagttcc ggccaaaaaa tagatactga
     3781 ttttgtcctt ttatctttag gtattaaacc agaagtaaat ctggctaaac aggccgggat
     3841 tggaattgga aaaacaggtg caattgaagt aaatgaaaga atggaaacca atgtagaaga
     3901 tgtttatgca gccggggact gtgctgaaac aataaatcta ttgacagacc aaagggtctg
     3961 gatcccaatg ggctcgaccg ctaataagca gggccgagtt gccggtgaaa atgcggccgg
     4021 gggagataac caccacttag gggtattcca aactgcgatc actaaaatat ttgattatac
     4081 agttgctaaa actggaattg atgagaaaga ggcggctgaa aatggttatg atcctgtagt
     4141 cgttactata actggtgcta accatgcgca ttacttcccg aactttaaaa agatgaagat
     4201 taggggtgta ttcgataaaa agagccatcg tattattgga gctcaaatag aaggagagag
     4261 caaggtcgac aaacgtatag atgtaatcag tacggccatc tatagcgaac ttagggctga
     4321 agatctcttt cagattgatc tagcctatgc cccaccttat tccactccga aagatccagt
     4381 agcggtctta ggcatggtgg cagagaaaaa gttaaatcaa tagataaata taaaaaaaag
     4441 aaagtgaccc gatattttgg gtcacttttt tttacattat tagatcatta tttacttact
     4501 aagtcctgca tttgttcata ggttaaagcc ccactgtgtc tagcctgtat aatactatct
     4561 ttgtctaata taaaggtagt agggataccg cggaccagat atttagaaga agcaactgta
     4621 ccgcttttgt ctaaaagggt agtgaatgaa aaattatttt tcattagata atcaaaaact
     4681 ttatctttgc cctcctgaat attaacggtc agcaccttta tattctcatt atttttatag
     4741 agtttttgaa tggccggcat ttctgcctta cagggcggac accagctggc ccagaaattt
     4801 aaaaatactt tctgcccctg taagtcatat aagcttattt tttctccact catatcttcc
     4861 aaggtaaaat caggtgcctt cataccgacc tgagccccaa ccttaacatc atcagaggct
     4921 gcctgtattt cgttaacata aaacaatgta cctgccgtta ataacagtaa agctattacg
     4981 gttattaaac ctttgttctt cattaatttc atagtaaaga cctcctaaaa gccaattata
     5041 tttagattgt ttgtaaaaat taaaatacct aaaattataa tcaatgcacc actcaccata
     5101 tttatataat ttaaatatct attcattttt ttaatcgccg gtaaaatctt tttggctgct
     5161 aaggctgtga ttaaaaaggg taaagcaaag ccaaaagagt aaatgaataa taaaaagccg
     5221 ccggtcatta agttctgact gttaccggct aaaattaaga ttgaagagag aatgggtcca
     5281 atacaaggag tccaggccaa agctattccc actcccagga taaaggactg cacatactta
     5341 ttagatcttt tggtaatatt tagctttttc tcataataaa gccattttaa tttgataata
     5401 ccg
//
LOCUS       DATGCJ010000110        11376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_192304, whole genome shotgun sequence.
ACCESSION   DATGCJ010000110 DATGCJ010000000
VERSION     DATGCJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192304"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..802
                     /locus_tag="VKN64_05415"
     CDS             185..802
                     /locus_tag="VKN64_05415"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMA59713.1"
                     /translation="MYLDLIKKRIKNSQIKPLGVKAKFAVLVPLLRLEDKLHLIYEIR
                     SEEIGTQPGEVSFPGGRIEEGETAREAAVRETEEELGLDKKQIEVFGQMDYLIPPFNI
                     ALYPFTGFLKVDSLADLAINKAEVQEIFTVPLNYFLENEPETHVVNCRHEINENFPYH
                     LIRNGKEYDWREGEYPVNFYKYKDHVIWGMTARFTTNLIDKIKNQ"
     gene            822..1229
                     /locus_tag="VKN64_05420"
     CDS             822..1229
                     /locus_tag="VKN64_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMA59714.1"
                     /translation="MEKKLYRSRESRVIGGVCGGIGEYFEIDPVIVRLVFLILFFVFG
                     VGLITYIIAWIIIPDKPLDQAEVEYEIDEEAIKKQKEKRMKVLGYILFGLGIFFILEL
                     WFDINFELGAEFWSIGLILIGLVLIFKHQSKNK"
     gene            complement(1232..2602)
                     /locus_tag="VKN64_05425"
     CDS             complement(1232..2602)
                     /locus_tag="VKN64_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMA59715.1"
                     /translation="MGTDPIVPLLFRLSIPSIIAMAIQALYNVVDSIYVGRLGTDPLS
                     ALSLSFPLQMIIIAIAVGTGVGTSSLISRRLGQGEREEAANAAEHVILISLFYSIIMA
                     LIGYFLSDRLFVIFTNDANLINLGTQYISIILMGSIALFFPMISNNILRGEGNTFIPM
                     VVMVIGAVINIILDPFLIYGLWIFPRLGIQGAAIATVTARLLSGIFLVFMLFSDKNEL
                     KMDLKTFEFDFNVIVEIYKVGFPAMIMQFLASFMLAGMNKILASFTTTAIAAAGIYFR
                     LQSFVFMPVFGLNQGYMPIVGYNFGHNNPKRMMKTIKLAGVIAFVFTTAGFIIFQTIP
                     TQLITLFNKDPELIRIGTTALKRISIAFPIIGPAIIISTTFQAMGKGLPSLFFSFLRQ
                     IIVLLPMMYLLGQWYGLNNLWYAFPISEVISVIPAAIWLKLTLNRVTSEMERRNNMPG
                     YSKDIG"
     gene            2725..3063
                     /locus_tag="VKN64_05430"
     CDS             2725..3063
                     /locus_tag="VKN64_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HMA59716.1"
                     /translation="MKEETTFETFNQLDIRVGKVKKVKDFPKANKPAYKLWVDLGEEI
                     GVKKSSAQITDLYSKEELKNTKVIAVVNFPPLQIADFISEILVLGIYREDGVVLLSPD
                     KEVEIGSKIG"
     gene            3085..4275
                     /locus_tag="VKN64_05435"
     CDS             3085..4275
                     /locus_tag="VKN64_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA59717.1"
                     /translation="MLDKKLIEEKAENIQTELIEWRRRIHQNPELGFKEYQTSDFIAA
                     KLNEYGIEYERVCETGIVGLIKGNKAGKTLAIRSDIDALALTEKNEVSYKSKNDGVMH
                     ACGHDGHTAILLGTAKVLNEMKDQIKGNIKLIFQPAEEGPGGAKPMLEAGVLEDPAVD
                     FILGLHINPGIESGKVGLKDGRIQAAPDYFEIKIKGKGGHGARPNEAIDSITIGSQIV
                     MGIQQIKSRETDTLKPLVISIGSFHSGEAFNAIPEEAVITGTVRSFDNDLRKMVKERM
                     EEIVKNITKAYRADYDFKYSFEYPPGYNNKKLTDLMREVIIEDLGEEYLYEIPEPSMG
                     GDDFAYFSQEVPGLYMRLGTRNEEKGTIHPLHSQNFNLDEDILKQGVKLFVYAANKIL
                     NNGQ"
     gene            complement(4258..5187)
                     /locus_tag="VKN64_05440"
     CDS             complement(4258..5187)
                     /locus_tag="VKN64_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMA59718.1"
                     /translation="MAYNIVISQILSLFLLIFLGYVLRKMNFINEKLNKGLSRILVDV
                     ALPALIISSMMVEINRELINYVKIFTIITAAIYLLIIILSEVIGKMAKLPLAKNTVFK
                     FILAFGNVGYMGIPIITTLFPENGIIFNSINLIFFNVLVWTYGVYLFNRKEEENYDFE
                     YKSLLNNGVIAIIIGFFLLFTGLRPPSFITGSLEMLGNITFPVSMLVIGSSLVNANFK
                     KLFLDKKLILLTIMRLILYPILTLLALSFFDLKPIIYQTSVILVAMPAPAQVVLFAEK
                     FDGDCKYASEGVFITTLFSVITIPFFLYLLTVI"
     gene            5223..7871
                     /locus_tag="VKN64_05445"
     CDS             5223..7871
                     /locus_tag="VKN64_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMA59719.1"
                     /translation="MYHIINQSVKGAIKINNLVTTIKDSCHECYACVRNCPVKALRVK
                     DGQSEVIKDRCINCANCVTICSQGAKEVKNHKEHIGSLINKEDMDLVVGLAPSFPAFD
                     TELNFEEWEEILIKSGFKEVYEVAWGAQLMINEYKEYLKKDDEPVISTACPVVVNLVR
                     KYYPELVNYLAPIVSPMGALVKYLRATVESDTKVVLIGPCHAKKMEFANNEFVEGVLT
                     YTEAFDMFENHFDIYKNKDKYDNKEEEEEDLIIDSYSRNIPIAGGLKEALEGKTDDRF
                     LKVEGGKKLNALFDMMSAGEISPKFVDALFCDGCINGVDLTEKSHFKKEIAVNKFIDH
                     NKRVDTKKYSINISNEINYQVSFEADYQKLEEPSEEEIWKILAKTNKYTEEDLLNCGA
                     CGYDSCKEKAKAVYQGLAEVEMCLPYLLNEKRNEVQDIRDLNLELDSIINSSYDGMLV
                     VDEDLNIERVNSSYLNMINLTEEELMDMDIRDLEEKKIIYPSAALLVFKEKRNITIVQ
                     NTSRKRLLTTATPVFSDNKIVRVIVNARDLKELDMAIDSEYSENEEVKLSLNKEDSKF
                     DDMTHIVCKSDDMKRILSIAEKIGNTNSSVLISGDSGVGKEVVARYIHQLNEKRDNFV
                     KINCGAIPERLLESELFGYETGAFSGARREGKPGLIEKANEGTLFLDEIGEMPLNMQV
                     KLLQVIQEHRVTRIGGVEQIEVDFRLITATNRDLKEMVKEGHFREDLFYRLNVIPINI
                     PPLTERKEDILPLFEYYVEVFSEKYDKEVKFGPEVKQILLTYEWPGNVREMINLLERL
                     IVTSEDTQIVTKDKIKGHLNPKNQTGSEQDIVVKDIVPLKEAVSLVEKELLKMARQEN
                     KSTYEIAEMLGVNQSTIVRKLNKYFE"
     gene            8248..8658
                     /gene="nuoE"
                     /locus_tag="VKN64_05450"
     CDS             8248..8658
                     /gene="nuoE"
                     /locus_tag="VKN64_05450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMA59720.1"
                     /translation="MKSDQFLAKAHDIQDTYGYIPEGEIDKLAKEFDVPRAKVYGVIR
                     FYSMFYTEPTGKYIIRVCDSLSCHINDSEDIVNTIQNYLGIEDGETTDNKKFTLEVVE
                     CLGHCGEGPVMMVNDKIYTHVNENMAIDILKDCT"
     gene            8679..9911
                     /locus_tag="VKN64_05455"
     CDS             8679..9911
                     /locus_tag="VKN64_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HMA59721.1"
                     /translation="MEERYFLKGDELKSVSEYDFPALKKALAMDQKEVIDEIDEAKLL
                     GRGGAGFPTGLKWKFAYQEESDTKYFICNGDEGEPGTFKDRYLMEDRPLKVLEGIMIG
                     AYAVGANKGYIYIRGEYAKSIKTFNKVIEDAYNEGILGENIFDSNFSFDLQLVKGAGA
                     YVCGDETSLINSIEGKRGRSRIKPPYPIQEGLFGKPTVVNNVETLTTAGEIINQGSAV
                     YKALGTENSKGTKLVCLSGDINEPGVYEVEFGSMTIKDIIEEFGKGIKNNGQLKFVIP
                     GGISTSILNKDQVDIPYTYEDIEEAESSLGSGALIVVAEGHDLVDLMLNASRFYMDET
                     CGTCFPCREGNRQVHHMLEEAKEKGIDQEKTDLIKDIGKTIRLASRCGLGQSSLNFIG
                     SVVDEYPGELLQGGANNG"
     gene            9904..>11376
                     /locus_tag="VKN64_05460"
     CDS             9904..>11376
                     /locus_tag="VKN64_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HMA59722.1"
                     /translation="MVNLEIDGQQVEVEEGTTILEASEKANIKIPTLCYEENLSIYGG
                     CRLCVVEIEGEDTLKPSCATMAEDGMKIRTHSPQVREVRKNLFELIIASHHIDCNLNC
                     LTCSRSQNCDLREIAADIGVSEIRLPPIDVGYDVDESSYSIVREPNKCITCGRCIRKC
                     DEIQSVGIFTTANRGPHTVVTTFEDKGIGNVECTNCGQCILACPTGALHEVYHYEHVW
                     DALHDEDKHVVVQTAPATRVAIGEPFGADPGTIATGQMVAALRRLGFDKVFDTNFTAD
                     LTIMEEGTELIERIKEGGTLPLFTSCSPGWIKFIEHQYPEFLDHVSSCKSPQQMFGAI
                     AKTYYAERNNIDPEDLVVVSVMPCTAKKFELDREEMSQDVDYSLTTRELARMIKQAGI
                     DILNLENEDYDELLGVSSGAADIFASSGGVMEAALRTAYEIITGEELGKVDFEQVRGL
                     EGLKEGSVKINDMEVKVAVAHGLGNARRILERMKDGEEYHF"
BASE COUNT         4132 a         1588 c         2305 g         3351 t
ORIGIN      
        1 tagttattcc cgaacacgta tggaatgtat tcaaaaggcc tatctataat gtagacctat
       61 ggcattttga gtaaatacag gtcataatta aatatatcaa ttaaaagaca gccccttacg
      121 ggttgtcttt tttctttgaa atatattata atataataaa tgcaatatct atgaggtgaa
      181 atttatgtat cttgacttaa taaaaaaacg cattaaaaat agccaaataa aaccattggg
      241 tgtaaaggca aaatttgctg ttttggttcc tttgcttaga ctagaagata aattacattt
      301 aatttatgag attaggtcag aagagattgg tacccaacca ggtgaggttt catttcctgg
      361 cggtagaata gaggaaggtg aaacggcccg cgaggctgcc gtgagagaaa cggaagaaga
      421 acttggactt gataaaaaac aaattgaagt atttggacag atggactatt tgataccgcc
      481 ttttaatata gcattatatc catttacagg gtttttaaag gtcgatagtc tggctgattt
      541 agccataaat aaggcggaag tacaagaaat attcacagta cctttaaatt actttttaga
      601 aaatgaacca gagacccatg tagtcaactg tagacatgaa ataaatgaaa actttcctta
      661 tcatttaatc cgcaatggta aagagtacga ctggcgggaa ggagagtatc cggtcaactt
      721 ttataaatat aaagatcatg ttatttgggg tatgacagcc cgttttacaa ccaatttaat
      781 tgataaaata aagaatcaat gaattttaaa ggagggttat gatggaaaag aaattatacc
      841 gttcccgtga aagtagagtt atcgggggag tatgtggtgg aataggtgaa tactttgaaa
      901 ttgatccggt tatagtgagg ctcgtatttt taatactctt ttttgttttt ggtgtgggtc
      961 taataaccta tatcatagcc tggattataa tcccggacaa accattagat caagcagaag
     1021 tggaatatga aatagatgaa gaagcaataa aaaaacaaaa agaaaaaagg atgaaagtcc
     1081 tcggctatat cttgttcggc ctcggaatat ttttcatcct ggaactatgg tttgatatca
     1141 attttgaatt aggagctgaa ttttggtcaa tcggtttaat cttgattggg ttagttttaa
     1201 tctttaaaca ccaatctaaa aataaatagg tttaccctat atcttttgaa tagccgggca
     1261 tattatttct tctttccatc tcagaggtaa cgcgatttaa ggttagtttt aaccagattg
     1321 cagcaggtat tacactaatt acttctgaaa tcggaaaggc ataccataaa ttattgagtc
     1381 cataccattg acctaaaaga tacatcatag gtagtaaaac gataatctgt cgtaaaaagg
     1441 agaagaacag actaggtagt cctttaccca tcgcttgaaa agtggttgaa attataatag
     1501 cgggaccaat aatcggaaag gcaatactaa ttctttttaa ggctgtagtt ccgattctta
     1561 ttaattccgg atctttgtta aataaagtaa tgagttgagt tggtattgtt tggaatatta
     1621 taaaaccagc ggtggtaaag acaaatgcta taactcccgc taatttaata gttttcatca
     1681 ttctttttgg attattatga ccaaagttat agccgacgat cggcatataa ccctggttta
     1741 aaccaaaaac aggcatgaag acaaaagact gaagtctaaa gtaaataccg gccgctgcga
     1801 tcgcggtggt ggtaaaggaa gccaagattt tattcattcc ggctaacatg aaactagcta
     1861 aaaactgcat tatcatggct gggaaaccga ctttatatat ttcaacaata acattaaaat
     1921 caaattcaaa ggtttttaga tccatcttaa gttcattttt atcactaaac agcataaata
     1981 ctaaaaatat tccacttaat aatctagcag taacggtagc tatcgctgct ccctggatac
     2041 caagacgagg gaatatccag agtccgtaga ttaaaaaggg atctaagatg atattgataa
     2101 ctgctccgat taccattaca accatgggaa taaatgtatt accttctccc cttaaaatat
     2161 tgttagagat catggggaag aataaggcaa ttgagcccat aagaattata gagatatatt
     2221 gggtacctaa atttattagg ttggcatcat tagtaaaaat aacaaagagt ctatctgata
     2281 aaaagtaacc gattaaggcc attattatac tgtaaaacag tgagatcaat attacgtgtt
     2341 cagccgcatt ggcagcctct tctctttcac cctgaccgag ccgccttgag attaaagaac
     2401 tggtgcctac tccagttccc acagcaattg cgatgataat catctgcagg gggaaactta
     2461 aggataaggc tgataaggga tcagtgccca gacggcccac ataaatactg tccacaacat
     2521 tatataaagc ttggatggcc attgctatta tagagggaat tgataatcta aataataagg
     2581 gtacaatcgg atctgtaccc attctttttg aacttcgttt ttgcacagaa atcacctcaa
     2641 gaaaatcctg ttaatctgtg taaatgatat attattatta atttattgtc aatgagatta
     2701 ccttaaaata aggggaataa aaaaatgaaa gaagaaacta cttttgagac ctttaatcag
     2761 ttagatatca gagttggtaa agtaaagaaa gttaaagact ttcccaaggc caacaaacct
     2821 gcctataaat tatgggtaga tttaggtgaa gaaataggag taaaaaaatc tagtgcccag
     2881 ataactgacc tgtattctaa ggaagaatta aaaaatacaa aggttattgc agttgttaac
     2941 tttcctcccc tgcaaatagc tgatttcatt tcagaaatat tagtattagg tatctataga
     3001 gaggatggag tagtactttt aagtcctgat aaagaagtgg aaataggttc aaaaatagga
     3061 tagataaaga aaggaagttt taacttgcta gataaaaaat taattgaaga aaaggcagaa
     3121 aatatacaga ccgagctaat agaatggaga agaaggatcc atcaaaatcc agaacttggt
     3181 tttaaagaat atcaaacctc tgattttata gcagctaaac taaatgaata tggtatcgaa
     3241 tatgaacggg tctgtgaaac gggtatagtt ggacttatta aaggaaataa agctggcaag
     3301 accctagcaa ttaggtccga tatcgatgct ttagccctga cagaaaaaaa tgaagtaagt
     3361 tataaatcaa aaaatgatgg ggttatgcat gcctgtggac atgacggcca tactgctatt
     3421 cttttaggaa cagcaaaggt tttaaatgaa atgaaggatc aaattaaggg taatatcaaa
     3481 cttatctttc aaccggctga agagggtccg ggtggggcaa aaccgatgtt agaagcgggg
     3541 gtattagaag atccagccgt ggacttcatt ttaggcctgc atattaatcc tggcattgaa
     3601 tcgggtaaag taggtctaaa agatggtaga attcaagctg cccccgatta ttttgaaata
     3661 aaaatcaaag gaaaaggtgg acatggggcc cgcccgaatg aagctattga ttctatcaca
     3721 attggttctc aaatagtaat gggaattcaa cagattaaaa gcagagaaac agatacttta
     3781 aagcctttag tgataagtat tggttctttt cactccggtg aagcttttaa tgcaatacca
     3841 gaagaagctg tcattaccgg gacagttcgt tcctttgata atgatttaag gaaaatggta
     3901 aaagagagaa tggaagaaat agtaaaaaat attaccaaag cctacagagc ggactatgat
     3961 ttcaagtatt catttgaata tccgcccggt tataataata aaaaactgac cgatttaatg
     4021 agagaagtaa taatagagga tttaggagaa gagtatcttt atgagatacc ggaaccttct
     4081 atggggggag atgactttgc ctacttttca caagaagtac caggacttta catgaggtta
     4141 ggaaccagga atgaagagaa aggtaccata catccacttc attcccaaaa ctttaattta
     4201 gatgaagata ttttgaaaca gggagttaaa ctttttgttt atgcagctaa taaaatttta
     4261 aataacggtc aataaataga ggaagaatgg tattgtaata actgagaata aagttgtaat
     4321 aaatactcct tcggaagcat atttacaatc accatcaaat ttttctgcaa acaatactac
     4381 ctgggcaggg gctggcatag ctactaatat tacacttgtt tgatatataa tgggttttaa
     4441 atcaaaaaat gaaagggcta ataatgttaa tatcggatat aatattaatc tcataatggt
     4501 taacaatata agttttttat ctaaaaataa ttttttgaag ttagcattaa ccagtgaact
     4561 accgatgact aacattgaaa caggaaatgt aatattaccc agcatttcaa gtgaaccggt
     4621 aataaaagaa ggtggtctta gaccggtaaa taataagaag aaaccaataa taatggcaat
     4681 taccccatta tttaaaagac tcttgtattc aaaatcataa ttttcttctt cttttctatt
     4741 gaataaatat actccgtatg tccacactaa aacattaaaa aagattaaat ttattgagtt
     4801 gaatataatt ccgttttcgg gaaataaagt tgtaattatc gggatgccca tataacctac
     4861 attcccgaat gcaaggatga atttgaatac agtgttttta gctaaaggca gtttggccat
     4921 tttaccgata acctctgata agataataat caaaaggtat atagctgcag taataatagt
     4981 gaaaatttta acgtaattta ttagttccct attgatttct accatcatcg aagatataat
     5041 taaagcaggt agtgccacat ctactaaaat acgcgacaag cctttattta atttttcatt
     5101 tataaaattc atctttctca atacatatcc aagaaatatt aaaagaaaaa ggcttaatat
     5161 ctgagagatt actatattat atgccatttt ttaacctact ttcctaatat tatttcaaat
     5221 aattgtatca tattataaat cagagtgtca aaggggcgat taaaattaat aatttagtaa
     5281 ctactatcaa agattcctgt catgagtgtt atgcttgtgt aaggaactgt cctgttaagg
     5341 ccctgagagt aaaagatgga caatcagaag taattaaaga tcgctgtata aattgtgcta
     5401 actgtgtaac tatttgctca cagggggcaa aagaggtaaa aaatcataag gaacatattg
     5461 gttcattaat taataaagaa gatatggact tagtggttgg tctagcacct agctttccag
     5521 cttttgatac cgaattaaat tttgaagaat gggaagagat ccttattaaa tcaggcttta
     5581 aagaggtata tgaggtagcc tggggagctc agctaatgat taatgaatat aaagagtact
     5641 taaaaaagga tgatgagcca gtaattagta cagcctgccc ggttgttgta aatttagtca
     5701 ggaaatatta tcctgaatta gtaaattatt tagcaccgat tgtatctcct atgggtgccc
     5761 tggttaaata tttaagagca actgtagaat cagatacgaa agtagtatta attggtccct
     5821 gtcatgctaa aaaaatggaa tttgccaata atgaatttgt ggaaggtgtt ttaacatata
     5881 cggaagcatt tgatatgttt gaaaaccatt ttgatattta taaaaataaa gataaatatg
     5941 ataacaaaga agaggaagaa gaggatttaa ttattgatag ttattcacgg aacataccaa
     6001 tcgccggtgg tttgaaagaa gcgttagaag gtaaaacaga tgaccgcttt ttaaaagtag
     6061 aaggcgggaa aaagttgaac gcgctctttg atatgatgtc agccggagaa attagtccta
     6121 aatttgttga tgccttattt tgcgatggct gtataaatgg agttgattta acggaaaaga
     6181 gtcattttaa gaaagaaata gccgttaaca aatttattga tcataataaa agagttgata
     6241 ctaaaaagta tagtattaat attagcaatg agataaatta ccaggtgagc tttgaagcgg
     6301 attatcaaaa attagaggaa ccctcggaag aagagatctg gaaaatttta gctaaaacca
     6361 ataaatatac cgaggaagac ctcttaaact gtggggcctg tggctatgat tcttgtaaag
     6421 aaaaagccaa agcagtttat cagggtttag cggaagtgga aatgtgttta ccatacttac
     6481 taaatgaaaa aagaaatgaa gtgcaggata tcagggatct taatctggaa ctagatagca
     6541 taataaattc ttcctatgat ggaatgctgg ttgttgatga agacctcaac atagaaagag
     6601 taaactcttc ttatctaaat atgataaatt taaccgaaga agaattaatg gatatggata
     6661 taagagattt agaagaaaag aagataattt atccttccgc agctttatta gtttttaaag
     6721 aaaagagaaa tattaccatt gtgcaaaaca ccagtagaaa aaggcttctt acgactgcaa
     6781 ctcccgtatt ttctgataac aaaatagttc gggtcattgt aaatgcaaga gatttaaaag
     6841 aactagatat ggccattgat agcgagtatt cagaaaatga agaggttaaa ctatctttaa
     6901 ataaagagga tagtaaattt gatgatatga cccatattgt ctgcaaatca gatgacatga
     6961 aaaggatctt aagtatagca gaaaaaatag gtaacacgaa ttccagtgtt ttgatctcag
     7021 gagattcagg tgttggaaaa gaagttgttg cccgttatat tcatcagtta aatgaaaaga
     7081 gagataattt tgttaagata aattgtggcg ccattcccga gagactatta gaatctgaac
     7141 tatttggtta tgaaacaggt gcctttagcg gtgcgagaag agaaggtaaa cctggtttaa
     7201 ttgagaaggc aaatgaaggg accctgtttt tagatgaaat aggtgaaatg cctttgaaca
     7261 tgcaggtgaa attactgcag gtcatccagg aacatagggt aacaagaatt ggtggagtag
     7321 aacaaattga agtagatttt agacttatta cagctacaaa tagagatcta aaagaaatgg
     7381 ttaaagaagg acattttcga gaggatcttt tctacagatt aaatgtaata ccaattaata
     7441 tccctccttt aactgagaga aaagaagata tattacccct ctttgaatat tatgttgaag
     7501 tttttagtga gaaatatgat aaagaagtca aatttggacc ggaagtaaaa cagattttat
     7561 taacctatga atggcctggt aatgttcgag aaatgattaa cttacttgaa aggctgattg
     7621 taaccagtga ggacactcaa attgttacaa aagataagat aaaagggcat ctaaatccca
     7681 aaaatcaaac tggaagtgaa caggatatag tcgtaaaaga tatagttccc ttaaaagaag
     7741 cagtgagttt agtagaaaaa gagcttctca aaatggcccg tcaagaaaac aaatcaactt
     7801 atgagattgc tgaaatgtta ggagttaatc agtctacaat agtaagaaaa ctaaataagt
     7861 attttgaata atgcattcct gcatcatatt aatgcatatc tgcattaatc atggttagtg
     7921 gaatgctagt ttttttagag atatttgtga aaaaaatcat ttatagtgat gcaaaaatgc
     7981 attattttaa acaaataaac tcttaatagt agtattcaag atcaaaacag gcaatatatt
     8041 gtgcatttat gtacaaaacc gagtacgaat ttggctgaaa tcaggtaaaa accctctttt
     8101 ttacgaaaaa attaagtttt tttaatcatt ttgtgcaatt tggcacaaga aatgcatata
     8161 aatataaagt ggaaagataa aaaatgaagg taacattttt ttaaaaactt aagagaaaac
     8221 tttcacaaaa aggagggtaa acagtaaatg aagagtgatc aatttttagc caaggcacat
     8281 gatattcagg acacttatgg ttatattccg gaaggtgaaa tcgacaaatt agccaaagaa
     8341 tttgatgtac cacgtgcgaa agtatatggt gttattcgct tttattcaat gttctatact
     8401 gaaccgactg gaaaatatat aataagggtc tgtgacagtc tatcctgtca tattaatgat
     8461 tctgaagata ttgtaaatac cattcagaat tatttaggaa ttgaagatgg tgagacaact
     8521 gataataaga agtttacact ggaagtagtt gaatgtttag gccactgtgg tgaaggtcct
     8581 gtgatgatgg taaatgataa gatttatacc catgtaaatg aaaatatggc tattgacatt
     8641 ttaaaagatt gtacataaaa ggagggaagc tgataaaaat ggaagaaaga tacttcttaa
     8701 aaggtgatga attaaaaagt gtttcagaat atgactttcc tgctttgaaa aaagcccttg
     8761 caatggatca aaaagaagtt atagatgaaa tcgatgaagc aaaattattg ggaaggggtg
     8821 gagcaggatt tcccactggt ttgaaatgga agtttgccta tcaggaagaa tcagatacta
     8881 aatactttat ctgtaacggt gatgaaggag aaccgggtac atttaaagac cgttacttaa
     8941 tggaagatag gcccttaaaa gtattagaag gaattatgat tggcgcttat gctgttggtg
     9001 ccaataaggg atatatctat attagaggtg agtatgctaa atccataaaa acatttaata
     9061 aagtaataga agatgcctat aacgaaggta ttttaggtga gaacattttt gatagtaatt
     9121 ttagttttga tttacagctg gttaaaggag caggtgctta tgtatgtgga gatgaaactt
     9181 ctttaattaa ttcaattgag ggtaaaagag gtcgttcgcg gatcaaacct ccttatccca
     9241 ttcaagaagg attatttggt aaaccgactg ttgtaaataa tgtagagact ctaactactg
     9301 cgggagaaat aattaatcaa ggttcagctg tttacaaagc attaggaacc gaaaacagta
     9361 aaggaacaaa actagtttgc ctaagtggag atatcaatga accgggtgta tatgaagttg
     9421 aatttggcag tatgaccatt aaagatataa ttgaagaatt tgggaagggg attaaaaaca
     9481 atggtcaact gaaatttgtt atacccggtg gtatctctac ttctattctc aataaggacc
     9541 aagttgatat tccttatact tatgaagata ttgaagaagc agaaagcagt ttgggatcag
     9601 gagccttaat tgttgtggct gaaggccatg atctagttga tttaatgctt aatgcttcca
     9661 ggttttacat ggatgaaacc tgtggaacct gtttcccctg tagagaaggt aatagacagg
     9721 tacaccatat gttggaagaa gctaaagaaa aaggaatcga ccaagaaaaa actgatctaa
     9781 ttaaagatat aggaaagaca ataagattag catctcgctg tggattagga cagagttcat
     9841 tgaactttat cggttcagtg gttgatgaat atccaggcga actgctgcaa ggaggtgcaa
     9901 ataatggtta atttagagat agatggacaa caagtagaag tggaagaagg aactaccata
     9961 ttagaagctt ctgaaaaggc taatattaaa ataccaactc tttgttatga agaaaatctc
    10021 tcgatttatg gtggatgtag gctatgtgta gttgaaattg aaggggaaga tactttaaaa
    10081 ccttcctgtg ctacaatggc tgaagatggt atgaaaatta gaactcactc tccccaggta
    10141 agagaggtta ggaaaaatct ctttgaatta ataattgctt cacaccatat tgactgtaat
    10201 cttaactgtt taacctgtag tcgttctcag aactgtgact taagagaaat tgctgctgat
    10261 atcggagtaa gtgaaataag gcttccacct attgatgttg gatatgatgt tgatgaatcc
    10321 agttattcta ttgtacggga acctaataaa tgtattacct gtggtaggtg tatcagaaaa
    10381 tgtgatgaaa tacaaagtgt tggtatattt actacagcca atagaggccc tcatactgta
    10441 gtaaccacct ttgaagataa gggtataggt aatgtagaat gtacaaactg cggccaatgt
    10501 attttagcat gtccaaccgg agctttacat gaagtttacc attatgaaca tgtttgggat
    10561 gcattacatg atgaagataa acatgtagtt gttcagactg caccagctac aagggtagca
    10621 ataggagaac ccttcggagc tgatccagga acgattgcaa ccggtcaaat ggttgctgct
    10681 ttaagaaggc taggttttga taaagtattt gatacaaact tcactgcgga tttaacaata
    10741 atggaagaag gaacagaatt aatcgagaga attaaagaag gtggaacatt accgctcttt
    10801 acttcttgta gtccaggctg gattaaattc atagagcatc aatatccaga atttttagat
    10861 catgtatcaa gctgtaaatc acctcaacag atgtttgggg caatagcaaa aacttattat
    10921 gcagaaagaa ataatatcga tccagaagat cttgttgtag tatctgtaat gccctgtact
    10981 gctaaaaagt tcgaattaga tagggaagaa atgtcgcaag atgttgatta ttccttaact
    11041 acaagagaat tagctcgtat gattaaacag gcaggtatcg atattttaaa tctagaaaac
    11101 gaagattatg atgaattact gggtgtctct tcgggagcgg ccgatatatt tgcttctagt
    11161 ggtggtgtta tggaagctgc cctcagaact gcctatgaaa taattactgg agaagaacta
    11221 ggtaaagttg attttgaaca ggttagaggt cttgaaggtt taaaagaagg gtcagttaaa
    11281 ataaatgata tggaagttaa agttgctgtc gcccatggct taggtaatgc aaggcgaata
    11341 ttagaaagaa tgaaagatgg agaagagtat cacttt
//
LOCUS       DATGCJ010000111         9117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_104357, whole genome shotgun sequence.
ACCESSION   DATGCJ010000111 DATGCJ010000000
VERSION     DATGCJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104357"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2330)
                     /locus_tag="VKN64_05465"
     CDS             complement(<1..2330)
                     /locus_tag="VKN64_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HMA59723.1"
                     /translation="MSKKKGRYIIVALILIISLGVGVFVGAITWIIKDTPDISNYKGS
                     SETTIIYSADGDLLTKLYRENRIYVSIDKIPAHLKEAIVAIEDKNFYVHHGVDFFGIV
                     RAFVANIRAGYRAQGASTITQQLARNALLHQRKTYYRKIQEAYLALQFERMYTKPEIL
                     EMYLNEIFMGHSAYGMEAAAQQYFGKSVSELSLSECALLAGLPRAPNYYSPYNNKDAA
                     LERRNIVLNKMVELGYISEESAQEAKNQELALESTLYETDEDTAPYFVRYVRDTLLDM
                     YGANMVYGSGLKVYTTLDTDMQEIANKSVENALEQKYIPTVQRDNAADPNQPQMALIT
                     LDAKTGAIKAMIGGRGNDQFNRATQAVRQPGSAFKPFVYTTAVKNNYSPGDVINDMPM
                     PNPKKNNQNQKEIWPTNYENKYRGMVSLRSGLVHSINVAAVKLMQKVSVNDTISTARQ
                     MGISTFEKADNVEDHYSLALGGLNRGVKPIEMATAYGIFANSGIRTEPFAITRVLDSN
                     NRVIYEAHPKKQIILSPETSYLMTDMLRSVINEGTGWRADLDRPVAGKTGTTNNYTDA
                     WFVGFTPSLVTSVWIGEDNLQQMVYDQRDENGNLIFTENGRARTISSAEAAQLWGDYM
                     REVVKGREVSYFSEPQNIKSVSIDPVTGKLPNKYTPRITEELFKTENAPRQAENLHGP
                     TETVKIDKTTGMLATENCPADNVVEYTYIEKSGVRVGPTEIHFSERNDGSKNADDLVK
                     GVYMVNEGEPIQKINHELGIPQTDGNGNTVYQTKPT"
     gene            2509..3729
                     /gene="tyrS"
                     /locus_tag="VKN64_05470"
     CDS             2509..3729
                     /gene="tyrS"
                     /locus_tag="VKN64_05470"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMA59724.1"
                     /translation="MDINKQLEILKQGVSDLISVEDLKKKLKEAEQEDRPLRVKLGLD
                     PTAPDIHLGHTVVLKKLKQFQDLGHEVYLIIGDFTGRIGDPSGKSKMRKQLTKEEIEA
                     NARTYEKQFSKILDEDKTKLVFNSSWLGEMDFADVIRLSSKYTVARMLERDDFHKRYE
                     DNKPIGIHEFFYPLMQGYDSIAIEADVELGGTDQRFNLLVGRNLQKEYGQEPQVLVMM
                     PILEGLDGTEKMSKSLDNYIGIEDGPSEMYGKVMSIPDDLIVRYYELVTNVDVDKVKQ
                     IKEDIEVNNVNPMQHKKDLAYTIVKEYHGEAKAQEAAQEFENVFSKGGMPEDIPEVEI
                     SQDDLEDGELWIVKIVAATGMVDSNSEARRMIKQGAVRINDKKYEKINLDLEVEDGMI
                     VQIGKRRYAKLRVK"
     gene            3745..6369
                     /gene="alaS"
                     /locus_tag="VKN64_05475"
     CDS             3745..6369
                     /gene="alaS"
                     /locus_tag="VKN64_05475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMA59725.1"
                     /translation="MTGDELRQKYLDFFKTKDHLILPSAPLVPQDDPSLLWINAGMAP
                     FKPYFDGRKEPPENRIATSQKCIRTNDIDNVGRTDRHQTFFEMLGNFSFGDYFKEEAI
                     VWGWEFVTEELGLDPDKLWITIYKDDEEAHDIWKDIVGLEEERIIRMGKKENYWQIGT
                     GPCGPCSEIHYDRGPEYGTSEEDVIGGEGDRYLEIWNLVFTQYNYTEDGEYLELPNKN
                     IDTGMGLERVASILQGVESNFETDLLYPMIEYLEADSGVPYKKNEETKMAYRLIADHI
                     RGVTMAIFDGVLPSNEGRGYVIRRLLRRAVRYGGKLGYEEPFLFKMVPVVIDTMSGGY
                     PNLEEKEEQIIKFVKAEEERFLNTLEQGLGILSEMIDEMEEEGKTELSGQKAFRLYDT
                     FGFPLDLTKDVLKENDLIVDEDGFEAEMAKQKQRAREAREEVGFGSSKEDELLKEISG
                     EIDKTEFVGYKHMSNQAQILKIIKDGELVEKLKEGEEGEIILDKSSFYAESGGQIGDK
                     GTIASSDSKAVVKDTVSKLDFFLHFSEVQSGSLEVGDRVTLEIYEDNRRATMRNHSAT
                     HLLHQSLRDVLGDHVNQSGSLVEPERFRFDFTHFSALTPEELEEIEYRVNEQIINNLD
                     VKIEEMDIDKAKEKGAAALFTEKYGQKVRVVQMGDFSIELCGGTHVNRTGDIGLFKIV
                     NEGSIASGIRRIEAVTGFKALEYVNEREELLTKSAELLNAEAKEVPNKVNKLQDNIKE
                     LEKEIRSLKDKIAHSKTTDVMDEVEEINGVKVLTHKMQDVDNNALRKMADKLQNKMES
                     GIIVLASALDNKVLFVAKITQDLVDQGYNAGDIIGQVAQVAGGGGGGRPDMAQAGGSK
                     VDKIDEALAKAKEIIKEK"
     gene            6452..6682
                     /locus_tag="VKN64_05480"
     CDS             6452..6682
                     /locus_tag="VKN64_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HMA59726.1"
                     /translation="MRFKPTKEDMSEAGYVIKRVSKALEEKGYNPVNQIVGYILSGDP
                     AYITSYKDARTMIRTIDRDEIIEELVKNYLNR"
     gene            6751..7185
                     /gene="ruvX"
                     /locus_tag="VKN64_05485"
     CDS             6751..7185
                     /gene="ruvX"
                     /locus_tag="VKN64_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMA59727.1"
                     /translation="MRKLGIDYGSKYIGLAVSDRSNTIAHSKEVIKRTDLKKDLATIK
                     DYISDYEIDEIVVGMPTSLNGTRGPRAEKTQQFINFLNNHLDIEVTVWDERFTTLIAD
                     QSMIDADISRRKRKKMVDKIAAALILQNYLDYLNQKDGEEDE"
     gene            7178..7441
                     /locus_tag="VKN64_05490"
     CDS             7178..7441
                     /locus_tag="VKN64_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HMA59728.1"
                     /translation="MNEEGSYYVDEEAGELVLKNEGVEERFVIEDEVHFDGSKYLVLV
                     PADDDEDKEALVLKMVQEGDQSYLSIIEDEEEFQNVRERYLSK"
     gene            7438..8454
                     /gene="mltG"
                     /locus_tag="VKN64_05495"
     CDS             7438..8454
                     /gene="mltG"
                     /locus_tag="VKN64_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMA59729.1"
                     /translation="MKKAGIVFLIFIILITVSLKIEDLTSAVNKTDEENILIEIKKGA
                     SAQQIVDTLYMHGLIKSRLIFRAYIHLTETDQKLQAGYYYLNRSMDMFDIIDQLKKGG
                     NAVYKVTIPEGFTVQQVIERLAEKSKNNLEAYTEVLEDHRFEYTFIPEESDRLLYKLE
                     GYLYPNTYTIPLGFNAAETINVILHSFKNNVKAKIEESELNNKYDLNQILTIASLIER
                     EAKFDDEKPIIASVIYNRLNQDMKLQIDATVQYVLGESKERLLYEDLEVESLYNTYLH
                     KGLPPGPICNPGQASIEAALNPSETDFLFYFAENNGRHIFTESYEEHIRKQREIKNEG
                     SSDG"
     gene            8447..9070
                     /locus_tag="VKN64_05500"
     CDS             8447..9070
                     /locus_tag="VKN64_05500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMA59730.1"
                     /translation="MVENKIFTLWEDSEKLKKLEARANKEHVPIISREIANILKSIIA
                     IKEPKLILEIGTAIGYSTLWMAEYTEPETEIVTIERDEERFKLAKKNIKEFGFDKKVN
                     LKFGEAQEIVQYLRRKFDLIFIDAAKGQYLNLFKIAEEKITDNGVIICDNVLYKGLVR
                     DNQRVDHKIRTMIVNMRKFLIYLKNNKRFKTEILDIDDGITISSRRN"
BASE COUNT         2992 a         1556 c         2007 g         2562 t
ORIGIN      
        1 tgagttggtt ttgtctggta aacggtatta ccgttgccat cggtttgggg gatacctagt
       61 tcgtgattaa tcttttgaat aggttctcct tcattaacca tatatacacc cttaacaagg
      121 tcatcagcat tcttggaacc gtcatttctt tcactgaaat gtatttcagt cggacctact
      181 ctaacaccac ttttttcaat gtaagtgtat tcaactacat tatcagccgg acagttttcc
      241 gtggccagca tcccggttgt tttatctatc ttaacggttt ctgttgggcc gtgtaaattc
      301 tccgcctgac gcggtgcgtt ttcagtttta aataactctt cggttatacg gggcgtatat
      361 ttattgggca gtttaccagt aaccggatca atggagaccg atttaatatt ttgaggttca
      421 ctgaaataag agacttcccg tcccttgact acttcccgca tataatctcc ccataactga
      481 gctgcctcag cactagagat agtgcgggcc cgaccatttt cagtaaagat caagttgccg
      541 ttttcatccc tctgatcata gaccatttgc tggagattat cttccccgat ccagacactt
      601 gtgaccaggc tgggagtgaa tcctacaaac caggcatctg tatagttatt ggtcgtaccg
      661 gttttaccgg cgaccggtct atccaagtca gccctccagc cggtaccttc attaataact
      721 gaccttaaca tatcggtcat caagtaactt gtctctggac ttaaaatgat ctgtttttta
      781 ggatgtgctt catagattac ccggttatta ctatccaaaa ccctggtaat ggcaaatggt
      841 tcggttctga tcccgctgtt ggcaaagata ccgtaggcgg tcgccatttc aatcggctta
      901 acacctctgt ttaaaccacc cagtgccagt gaataatgat cttccacatt atccgccttt
      961 tcaaaggttg agatacccat ttggcgggcg gttgatattg tatcattaac acttaccttc
     1021 tgcattagtt taactgcagc tacattaatg gagtgaacga gaccggatct caaactaacc
     1081 atgccgcggt atttattttc atagtttgtc ggccagattt ctttctggtt ttgattgttt
     1141 ttcttcggat taggcatcgg catgtcattt atgacatcac cgggtgaata attattttta
     1201 acagctgtgg tatagacaaa tggtttaaag gccgaaccgg gctgtctaac agcctgagtg
     1261 gctcgattaa actgatcatt acccctacca ccaatcatag cttttattgc acctgttttg
     1321 gcatcaagcg taattaaggc catctgaggc tgattggggt cagctgcatt atctctctgt
     1381 acagttggta tatatttttg ttcaagcgca ttttcaacag atttattggc aatctcctgc
     1441 atatctgtat ccagggtggt atatactttc aggccactac cataaaccat attggcccca
     1501 tacatatcta ataatgtatc acgaacatat ctcacaaagt agggggctgt atcttcatca
     1561 gtttcataca aggtagattc cagggccagt tcttgatttt tggcctcctg ggcactttct
     1621 tcggagatat agccgagttc taccatttta tttaaaacta tgttgcgtct ctccagagcg
     1681 gcatccttat tattataggg tgaatagtag ttaggtgccc tcggtagtcc tgctaacagg
     1741 gcacattcac ttaaagaaag ctcggaaaca cttttaccga agtactgttg ggcagccgcc
     1801 tccataccat aggcgctgtg gcccataaag atttcgttta aatacatttc taagatttca
     1861 ggcttggtat acatcctttc gaactgtaag gctagatagg cctcctgaat cttacggtag
     1921 taggtttttc tctggtggag cagggcgttt ctggccagct gctgtgtgat agtactggct
     1981 ccctgggccc gataacctgc cctgatgttg gctacaaagg ctctcactat accaaagaag
     2041 tcaactccgt ggtgtacata aaagttttta tcttctatag ccactattgc ttctttaaga
     2101 tgggccggaa ttttatcgat tgatacatag atcctgtttt ctctatatag ttttgtaagt
     2161 aagtctccgt ctgcactata gataatagta gtttcactgg accctttata attgctgata
     2221 tcgggtgtgt ctttgattat ccaggtgata gcacctacaa agaccccgac tcctaaggat
     2281 attattaaaa tgagtgctac aattatatag cggcccttct ttttactcat aatattttac
     2341 cacctctcaa ttgcacatta tatcataaat tttaatatct tggtagcact tgttagaaac
     2401 aatccaatct gttataatta taaacagttt atattaaaca ttttaatagt agatagtaat
     2461 aaattcaagg ggaggtgtcc aatctgacat caatttgtca gattaaatat ggatattaac
     2521 aagcaattag aaatattaaa acagggtgta agtgatttaa tatcagtcga agacttaaaa
     2581 aagaaattaa aagaagccga acaagaagac agacctttaa gggttaaact gggtttagat
     2641 cccacagccc ctgacataca tttgggtcac accgtagtct taaagaaatt aaaacagttt
     2701 caggacttag gacatgaagt ttatctaatt attggagact ttacgggccg gatcggtgat
     2761 ccgagcggca agtcaaaaat gagaaaacag ctgaccaaag aagaaattga agccaatgca
     2821 cgtacatatg aaaaacagtt ttccaagatt ttagatgaag ataagacgaa attagtcttt
     2881 aactccagct ggctgggtga gatggacttt gcagatgtaa ttagactttc ttccaaatat
     2941 actgtagccc gtatgctgga aagagatgat tttcataaaa gatacgagga taataaaccg
     3001 attggtatcc atgaattttt ctatccctta atgcagggtt atgattcgat cgcaattgaa
     3061 gccgatgtag agttgggtgg aaccgaccag aggtttaacc ttttagtggg cagaaactta
     3121 cagaaagaat atggacagga accacaggtt ctagtcatga tgcctatctt agaaggttta
     3181 gacggaaccg aaaagatgag taagagcctt gataactata tcggaattga agatggacca
     3241 tctgaaatgt acggtaaggt aatgtcaatt ccggatgact tgattgtaag gtactatgaa
     3301 ctggttacca atgtagacgt agataaggtc aaacagataa aggaagatat agaagtaaat
     3361 aatgtaaacc cgatgcagca taaaaaagac ttagcctata caattgttaa agaataccac
     3421 ggggaagcta aggcacaaga ggccgcccag gagtttgaaa atgtcttttc caaaggtgga
     3481 atgccggaag atattccgga agttgaaatc agtcaagatg atttagaaga cggtgaactc
     3541 tggatcgtta agatcgtagc tgcgaccggt atggtcgatt ccaacagcga agcccgccgc
     3601 atgatcaaac agggtgcggt cagaattaat gacaaaaagt atgaaaagat aaatcttgat
     3661 ttagaagtag aagacggcat gatcgtgcag atcggtaaga gaagatatgc caagttaaga
     3721 gtaaagtaaa aagggggatt taagatgaca ggagatgaat taagacagaa ataccttgat
     3781 ttttttaaaa ctaaagatca cttaatttta cccagtgctc ccctcgtacc tcaggatgat
     3841 ccgagcttgc tctggatcaa tgccgggatg gctcccttta agccgtactt tgacggcagg
     3901 aaagaacctc cagaaaatag aattgccact tcacagaagt gtataaggac caatgatata
     3961 gataatgtgg gacgtactga ccgacatcag accttctttg agatgttagg taacttttcc
     4021 tttggagact actttaaaga agaggctatc gtttggggct gggaatttgt cacagaagaa
     4081 ttaggattag atccagataa actctggatt acgatatata aggacgatga agaagcccat
     4141 gatatttgga aagatattgt cggcttagag gaagaaagaa tcattagaat gggtaaaaaa
     4201 gaaaattact ggcagatcgg aaccggaccc tgtggtcctt gttctgagat ccattatgat
     4261 aggggacccg aatatggaac cagtgaagaa gatgtgatcg ggggcgaagg agaccgctac
     4321 ctagagatct ggaacctcgt atttacccag tataactata ccgaagatgg tgagtattta
     4381 gaactaccca ataaaaatat tgataccggt atgggattag aacgggttgc ttctatctta
     4441 cagggggtag agtccaactt tgaaaccgac ctcctgtatc ccatgataga atatttagaa
     4501 gctgacagcg gtgttcccta taagaaaaat gaagagacca agatggctta ccgcctcatc
     4561 gctgaccaca tcaggggagt aactatggct atctttgatg gtgtgcttcc ttctaatgaa
     4621 gggaggggct atgtaattag acgactgctc agaagggcag tccgctacgg cggtaaatta
     4681 ggttatgaag aaccattttt attcaagatg gtgccggttg tgatcgatac gatgtccggc
     4741 ggctatccga acttagaaga aaaagaagaa cagatcatca aatttgttaa agcggaagaa
     4801 gaaaggtttt taaataccct tgaacagggt ttaggtatcc tttccgaaat gatagatgag
     4861 atggaagaag aagggaaaac cgagctatca ggtcaaaaag ccttccgtct atatgatacc
     4921 tttggttttc cacttgatct aaccaaggat gttcttaaag aaaatgattt aattgttgat
     4981 gaagacggct ttgaagcaga gatggccaaa cagaagcaaa gggctcgcga agccagagaa
     5041 gaagtgggct tcggcagcag caaggaagat gaactcttaa aagagataag cggtgaaata
     5101 gataagaccg agtttgtggg ctataagcat atgtccaatc aagctcagat cttaaagatt
     5161 attaaagatg gtgaattggt agagaaatta aaagaagggg aagaaggcga gattatttta
     5221 gataagtcct ccttctatgc agaaagcggt ggccagatcg gtgacaaggg tacaattgcc
     5281 agcagtgact ccaaggctgt tgttaaagat acggtcagta aactagactt tttcttacat
     5341 ttttcagaag tgcagtcagg tagtttagaa gttggcgaca gggttaccct agaaatctat
     5401 gaagataacc gcagggcgac catgcgcaac cactccgcta cccacctcct acatcagtcc
     5461 ttaagagatg tattaggtga ccatgtaaac cagtctggtt ctttagtcga acctgaaagg
     5521 tttaggtttg actttacaca tttctctgcc ctcacacccg aggagttgga agagatcgaa
     5581 tacagagtta atgaacagat tatcaataac ttagatgtta agatagaaga gatggatatc
     5641 gataaagcga aagaaaaagg ggcagctgcc ctctttactg agaaatatgg tcagaaggta
     5701 agggttgtcc agatgggtga tttcagtatt gaactatgtg gaggtaccca tgtcaacagg
     5761 accggtgata tcggattatt taagatcgtt aatgaaggat ctattgcctc cgggatcaga
     5821 aggattgaag cagtgaccgg ctttaaggcc ttagaatatg taaatgagag agaagaactg
     5881 cttaccaaat cagccgaact tctcaacgct gaagcaaaag aagtacccaa taaagttaat
     5941 aagttacaag ataatattaa agaattagaa aaagagatta gatccttaaa agataaaata
     6001 gctcattcta agaccactga tgttatggat gaagtggaag agattaatgg ggttaaggtt
     6061 ttaactcata agatgcagga tgtagacaat aatgccctca gaaaaatggc tgataaattg
     6121 caaaataaaa tggaatcagg tataattgta ttagcctcag ctttagataa taaggtatta
     6181 tttgtagcca aaatcaccca ggatttagtt gaccagggct ataatgccgg tgatattatc
     6241 ggtcaagttg cccaagttgc cggtggtggt gggggcggac gtcccgacat ggcccaggcc
     6301 ggcggttcta aagtagacaa gattgatgag gctttagcta aagcaaaaga gattattaaa
     6361 gagaagtaat taaatttcaa ttttagtact aatactatta atatgggaag tggtataaat
     6421 gcctgaagat aataataaaa atgatcagac gatgcgtttt aaaccgacaa aagaagatat
     6481 gagtgaagct ggttatgtca taaaaagggt ttctaaagcc ctagaagaaa agggctataa
     6541 tccggtcaac cagattgtcg gttatattct ttcaggtgat cccgcctata ttacaagtta
     6601 taaagatgcc cggaccatga ttagaaccat cgaccgggat gaaataattg aagaattagt
     6661 aaaaaactat ttgaatagat aattatcaaa ataggtgctt ctaaaaaata tttttggaag
     6721 caccatttta acactttgga ggaatattaa ttgcgaaaat taggaattga ttacggcagt
     6781 aagtacatcg ggttggctgt gagtgaccgt tccaatacta ttgcccatag taaagaagta
     6841 attaaaagaa ctgatctaaa aaaagatcta gccacaatta aggactatat cagtgattat
     6901 gaaatagatg agattgtggt aggaatgccg acttctttaa acggaactag aggtccccgg
     6961 gcggaaaaaa cacagcagtt tattaacttt ttaaataatc atttagatat tgaagtaaca
     7021 gtctgggatg aacgattcac taccctgata gctgaccaga gtatgatcga tgccgatatc
     7081 agccgtcgga aaagaaagaa gatggtcgat aagattgcgg ctgctttaat actgcagaat
     7141 tacttagatt atcttaatca aaaggatggt gaagaggatg aatgaagaag gtagttatta
     7201 tgtagatgaa gaagcaggtg aattagtatt aaaaaacgag ggtgtagaag aacgttttgt
     7261 tatcgaagat gaagtacact ttgacggcag caagtacctt gtcttagtac ctgctgatga
     7321 tgatgaggat aaagaagctt tagtcttaaa gatggtacag gaaggtgacc aaagttattt
     7381 aagtattatt gaagacgaag aagaatttca aaatgttaga gagaggtatc taagcaagtg
     7441 aaaaaagccg gtatagtatt tttaatcttt attatcttaa taaccgtttc tttaaagatc
     7501 gaagatttaa ccagtgcggt taataaaacc gatgaagaga atatcttaat agagattaaa
     7561 aaaggggcgt cagcacagca gattgttgac accctttata tgcatggttt aatcaaatca
     7621 aggctgatct ttagagccta catccatctg accgaaaccg accagaaact ccaggccggt
     7681 tattactatc ttaataggtc gatggatatg ttcgatatta ttgaccaatt aaaaaagggc
     7741 ggtaatgcgg tctacaaggt tactataccg gaaggattta cggttcagca ggtaatagaa
     7801 agactggctg aaaaaagtaa gaataattta gaagcttata ctgaagtttt agaagaccac
     7861 aggtttgaat atacatttat tccagaagaa agtgatagat tattatataa attagaagga
     7921 tatctttatc ccaacactta tacgatacct ttaggcttta atgcagccga gactattaat
     7981 gtaatattac attcctttaa aaataatgtt aaagcaaaga tagaagagag cgagttaaat
     8041 aataaatatg atctaaatca aattctaaca attgcttctt taatcgaaag ggaagctaag
     8101 tttgatgatg aaaaaccgat tatagcctct gttatctata ataggcttaa tcaggatatg
     8161 aagttacaga ttgatgcaac ggtccagtat gtattaggag aaagtaagga gagattatta
     8221 tatgaagact tagaagttga gtctttatat aatacttatc tgcacaaggg tttacccccg
     8281 ggaccgatct gtaacccggg ccaggcttct attgaggcgg ctttaaatcc aagtgaaact
     8341 gactttctat tctattttgc tgaaaataac ggccgacata tctttacaga gagttatgaa
     8401 gaacatatcc gaaaacaaag agagatcaaa aatgagggat cgtccgatgg ttgaaaataa
     8461 aatatttaca ctatgggaag atagtgaaaa attgaagaag ttagaagcca gggcgaataa
     8521 agaacatgtt ccaattatat caagagagat tgccaatatt ttaaagtcaa taattgcaat
     8581 taaagaaccg aaattaattt tagaaatagg gacagcaatc ggttattcaa ccctctggat
     8641 ggccgaatat acagagcccg agacagagat cgttacgatt gaaagagatg aagagaggtt
     8701 taaactagct aaaaagaata ttaaagagtt tggttttgat aaaaaagtaa acttaaagtt
     8761 cggtgaagcc caggaaattg tacaatattt gcgtagaaaa ttcgatttaa tttttataga
     8821 tgctgccaag gggcagtatc taaatttatt taaaatagca gaagaaaaga taaccgataa
     8881 cggtgttatt atttgtgata atgtattgta caaaggtctg gttagagaca atcaaagagt
     8941 tgaccataaa attagaacga tgattgtaaa tatgagaaag tttttaatct atttgaaaaa
     9001 caataagcgg tttaaaaccg aaattttgga tatagatgat ggaattacta tcagcagcag
     9061 gaggaattga taatggataa agttgaacta ctggccccag ccggcaattt agaaaag
//
LOCUS       DATGCJ010000112         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_192357, whole genome shotgun sequence.
ACCESSION   DATGCJ010000112 DATGCJ010000000
VERSION     DATGCJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192357"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VKN64_05505"
     CDS             complement(<1..1496)
                     /locus_tag="VKN64_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMA59731.1"
                     /translation="MENKFKIVFALIVVISLTLVLSSCGFPVYFNHYSVSGTVQDSEG
                     KPIMNAAIKFDNGYSSTSSDEDGYWQKSGLGGSVIIRAEKAGHSFTPSQYPVNENETN
                     INFTSNQVVPEKYEISGKVEDGKGNGIGDIVIRFSDGYAPTTTDSSGNWEKDNLSGDI
                     TVTPDHNKYVFDPTSRSVSGPDSYVNFVARESSKTYSISGKVVDQQDNGISDVIIRFS
                     GGFESIKTDEEGNWEKDGLEGEVTITPEKRDYTFDPESRTVSGSDKYLNFVGTYADKS
                     YDIRGQVADNQDNGIEDVVIRFSGSYSPVTTDADGNWEKENLEGEVTVTPEKTDYSFD
                     PASRTVSGPDSYMDFVGTYADQPYDISGQVVDNQDNGIEDVVIRFSGSYSPVTTDADG
                     NWEKENLEGEVTVTPEKTDYSFDPASRTVSGPDSYMDFAGTYTNTDYYNVEGYVYLED
                     STEGVPGVEITFESMNLDKQYSTVVTDHEGKWSKTNLQGQVKIIPELEG"
     gene            complement(2322..>2629)
                     /locus_tag="VKN64_05510"
     CDS             complement(2322..>2629)
                     /locus_tag="VKN64_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59732.1"
                     /translation="LNKKLEDPCVFAGEEDNDNIYVKSDTTIVDDNGAYNLNQVQSGN
                     LYIYGWRDVNENGTIDDGDYYGRSNLLDIEGSSNYNVDIDMYYIDGSSGINLAVQGF"
BASE COUNT          859 a          504 c          324 g          942 t
ORIGIN      
        1 ccttctaatt caggtattat ttttacttgt ccctgtaaat tggttttcga ccatttacct
       61 tcatgatcag taactactgt gctatattgt ttatctaaat tcatgctttc aaatgtaatt
      121 tctactccag gtacaccttc agtactatct tcaaggtaaa cataaccctc aacattatag
      181 taatctgtat ttgtataggt gcctgcaaag tccatgtagc tgtccggtcc acttaccgtt
      241 cggctagccg gatcaaagct gtaatctgtt ttctccggtg taacagtgac ttcgccttct
      301 aagttctctt tctcccagtt accatcagca tctgtggtca caggtgaata actaccgctg
      361 aatctaatca ctacatcttc tataccgtta tcctggttat ctactacctg gccgcttatg
      421 tcatagggct gatccgcata agttcctaca aagtccatgt agctgtccgg tccacttacc
      481 gttcggctag ctggatcaaa gctgtaatct gttttctccg gtgtaacagt tacttcgcct
      541 tctaagttct ctttctccca gttaccgtca gcgtctgtgg tcacaggtga ataactaccg
      601 ctgaatctaa tcactacatc ttctatacca ttatcctggt tatctgctac ctggcccctt
      661 atgtcatagg acttatccgc ataagttcct acaaagttca aatatttatc tgaaccgcta
      721 actgttctac tctctggatc aaaagtataa tctctttttt ctggagttat agtaacttct
      781 ccttcaagac catctttttc ccaattaccc tcttcatctg tttttattga ttcaaatcca
      841 ccactgaatc taataataac atcagagatt ccgttatcct gctgatctac tactttacca
      901 cttatactat aagttttaga cgattctctg gccacaaaat ttacataact atctggcccg
      961 ctaactgaac ggctggtcgg atcaaataca tatttgttat gatcaggtgt aacagtaata
     1021 tctccactta aattatcttt ttcccaattg ccagaagaat cagtcgtggt tggtgcataa
     1081 ccatcactga atcttataac tatatctcct ataccattgc cctttccgtc ttcaaccttt
     1141 ccacttattt catatttttc gggaacaact tgattactgg taaaatttat attagtttca
     1201 ttctcattaa caggatattg agatggagta aaactgtgtc ctgctttttc tgcccttata
     1261 attacagaac ctcctagacc tgatttctgc caataaccat cttcatcgga agatgttgaa
     1321 ctataaccat tgtcaaattt aatagcagca ttcattattg gttttccttc ggaatcctgt
     1381 actgtacctg aaacactata atgattaaaa tatactggaa aaccacaact gcttagaact
     1441 aaagtgagag aaataactac aattaaagca aaaacaattt taaacttgtt ttccatagaa
     1501 aaaagcctcc cttaatttgg aatttaaaaa taaaaatgat aattttttta aaatttctta
     1561 attataaaca tatctatgta tatctgaacg tataatatat aattcaacaa ttgacaagat
     1621 tatccttcat cagacttaga tgtttttgat atataaagat aataagacta ttttaaacat
     1681 ttgaaaaact cattaaatga taactcatta aggtatatgt tctaataaaa tatattcatt
     1741 taatatggtt tttaatttca gaactcctaa agtgtaatat tttaactata taatattaat
     1801 aggtttccta tcatatcaat aatttataaa agaatatatt aatttttaaa atgatacttt
     1861 ttgtgcaggc ttgaaattag aaaatcatat aaatcttcat atattgggtt ttagcatcta
     1921 aaaagagtag ttcatcagta ttttttgcac aaaaagaaaa ttgcaaatat taggagctct
     1981 gaatttaaat ataattagaa tatatcataa taagaagtta acttcaataa cagttagtac
     2041 agaaattata aaattatgaa tgaatattat gaacacaaag tttccaaaaa caagttatca
     2101 atattcacaa ttactaattc ttggaaaaat ttataaatta acaataatat aataaatact
     2161 agttttgatt attttgatgg ttgaacataa cttgacaact caaatatatt tcaatacttt
     2221 ataaaataag aaggaaaata aatacactaa agaaacagaa tttatttctt tagctattat
     2281 tcccccatta tatttacttt atattttttt gatataattt attaaaaacc ctgtacagca
     2341 aggttaatgc cagatgaacc atctatataa tacatatcta tatctacatt ataattggag
     2401 ctaccttcta tatctaaaag attcgatctg ccgtaataat caccatcatc aattgtacca
     2461 ttttcattca catctcgcca tccatatata tataaatttc ctgattgaac ttgatttaga
     2521 ttatacgccc cgttgtcatc cactatagtt gtatcacttt taacatatat attatcatta
     2581 tcctcttcac ctgcaaaaac acagggatct tccaactttt tattaagta
//
LOCUS       DATGCJ010000113         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_415944, whole genome shotgun sequence.
ACCESSION   DATGCJ010000113 DATGCJ010000000
VERSION     DATGCJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415944"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..991)
                     /locus_tag="VKN64_05515"
     CDS             complement(269..991)
                     /locus_tag="VKN64_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HMA59733.1"
                     /translation="MSEEINVLVWSEYRQARDDEEVKKIYPAGINNFIAEFIKDDCIK
                     TSTAQLDQKDHGLTKEKLDETDVLIWWGHIAHDEVRDEIVERVYKKVLEGMGLIVLHS
                     AHFSKLFKKLMGTSCNLKWRVAEEVERLWITAPGHPIVKDIEDEYIELEKEEMYGEYF
                     DIPQPDDIIFISWFAGGEVFRSGLTYKRGQGNIFYFRPGHEEYPTYHNQKIQKVIKNA
                     VHWANNSHIRKINYGNSPNQIK"
     misc_binding    1148..1368
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1409..2329
                     /gene="cysK"
                     /locus_tag="VKN64_05520"
     CDS             1409..2329
                     /gene="cysK"
                     /locus_tag="VKN64_05520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HMA59734.1"
                     /translation="MNKDVIDLIGNTKMVKIDEMNKETNSDIFIKLEGTNPGGSIKDR
                     PVKKMFELAEKRGNLKPGDTVIEPTSGNTGIAIALISKVKGYNAILVMPETMTEERIN
                     LMKMYGAEIILTDGSKGMNGSIELAEKLRSENDYLMLDQFKNEGNYLAHYQNTGEEII
                     EQLMDIDIFAAGVGTGGTVTGVGKKLKEYNKGIKVVALEPANSAVLSGEEPGSHKISG
                     IGAGFIPEIMDTSVIDEVIKITDDEAFDHTKQLLDKEGLFTGISTGANIAGALKLAKR
                     YSNKKIVTVSPDNGWKYMSVLDYAKEDHND"
     gene            2322..2882
                     /gene="cysE"
                     /locus_tag="VKN64_05525"
     CDS             2322..2882
                     /gene="cysE"
                     /locus_tag="VKN64_05525"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HMA59735.1"
                     /translation="MIKYIKELVNYTLKKDPAAKSFWQILLLYPSVHAQIMYKVAHKL
                     YNGERYFTARLISQLARFLTGIEIHPGAELSNKVFIDHGHGVVIGETAEIGEDVVIYH
                     GVTLGATGKEKNFKRHPTIKNHVMVGAGSKVLGPITVHDGAKIGANAVVLDEVHRNQT
                     VVGMPAKAENKLRIIKDEEYKKVSAK"
     gene            complement(2903..3859)
                     /locus_tag="VKN64_05530"
     CDS             complement(2903..3859)
                     /locus_tag="VKN64_05530"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HMA59736.1"
                     /translation="MENKQLKTGKIGIVGVGSVGSTTAYALMIQGIGSEIVLVDRNKD
                     KAAGEAMDIMHGQSFVKPIEVRAGEYSDLKDAKVIIITAGAPQKEGETRLDLVQKNTN
                     IFKDIIGKITKYNQDAILLVVANPVDILTYVTLKLSGFPKNRVLGSGTVLDSARFRSL
                     ISSQCKIAAKNVHGYVIGEHGDSEVPVWSLTNIAGTQIDKYCPVCDQNCDGVVKERIS
                     EQVKNAAYDIIEKKGSTYYAVALGVAQITQAILRDEDTVLTVSSLLQGEYGIEDVSLS
                     LPTVINDKGIRNVLELDLNEEEQEAFIKSANLMKEVISNLNM"
BASE COUNT         1235 a          732 c          685 g         1236 t
ORIGIN      
        1 tgaaagttgg aatttcagtt ccgtataaat tggatagata aaataatgca gctaaagcaa
       61 aaataatcca aataaaaaat atgaatcttt tcattttttt caccccgaga ctatatatat
      121 ttatataaaa tagttagatt agttatgggg tataatgatt tattttttat atatttaatc
      181 ttagtataca caaattatag catattttaa caaaataatc tatatttata caacaaaagt
      241 tattattaaa tgtgaaattt attataacct actttatttg attcggtgag tttccataat
      301 taattttcct tatatgggaa ttattagccc aatgtacggc atttttaata accttttgaa
      361 ttttttgatt gtgataagta ggatattctt catgaccggg acgaaagtaa aaaatattac
      421 cctgacccct tttataggtt aagccgcttc taaaaacctc tccccctgca aaccaactta
      481 tgaagataat atcatctggt tgaggtatat caaaatattc tccatacatt tcttcttttt
      541 ctaattcaat atattcatcc tcaatatctt tgactatagg gtggccggga gcagtaatcc
      601 agagcctctc tacttcctca gcaactctcc attttaaatt acaggaggta cccattaact
      661 tcttaaataa tttagagaag tgagctgaat gtaaaacaat cagccccatt ccttctaaaa
      721 cttttttata taccctctct actatttcat cccttacttc atcatgggct atatggcccc
      781 accagatcag gacatcggtc tcatctaatt tttctttagt tagtccgtga tctttctgat
      841 ccagttgggc agttgatgtt tttatacaat catctttgat aaattcggca ataaaattat
      901 taataccagc tggatatatc tttttcactt cttcgtcatc tcttgcctgc ctgtattcac
      961 tccagaccaa tacgttaatc tcctcagaca taattttgcc cccttatatt agctaatttc
     1021 cttgttttta ttttaccatt tacctaaata tttttacaat ttagactttc ttttatttcc
     1081 tgcccatata cttgacaata ttagtcaact atattatact tattaacaga taatcagata
     1141 ttgatattta aaagctgtga acaaggaaag tattgaaaac agaaattttt gagagaggaa
     1201 accagctgct gaaaggtttc taaatttctt tttcgggaag tgcccttggg agctgtggac
     1261 cgaaccaagt aggttacaac ggtttttcac ccgttatcgt gaaagggtat gaaagtacct
     1321 aattaagaat aaatgtaaag tggaaccgcg agtttaaccg cctttatcaa aagggcggtt
     1381 ttttgtattt taaaggagga aaaaaagaat gaataaagac gttattgacc tgattggtaa
     1441 taccaagatg gtaaaaatag atgaaatgaa caaggagaca aattctgata tttttattaa
     1501 attagaagga actaatccgg gtggttcaat taaggaccgg cccgttaaga aaatgtttga
     1561 actggcagaa aaacgtggaa accttaaacc cggtgataca gttattgaac cgaccagcgg
     1621 caataccggg atagctatcg ctttaattag caaagttaaa ggctataatg caatcctggt
     1681 catgcccgaa accatgactg aagaacggat aaatttaatg aagatgtatg gagccgaaat
     1741 aatcttaact gacggtagta aaggaatgaa cggctcaatc gaactggccg aaaaactgcg
     1801 cagtgaaaat gactacctga tgctagacca atttaaaaat gaaggcaact atctagccca
     1861 ttatcaaaat acgggagaag aaattattga acagttaatg gatatagata tctttgcagc
     1921 cggtgttggt accggtggga ctgtgaccgg ggtcgggaaa aagctaaaag aatataataa
     1981 gggtataaaa gttgtcgcct tagaaccggc taattcagct gtcctttccg gtgaagaacc
     2041 cggttctcat aaaatatctg gtattggagc cggttttata cctgagataa tggatacttc
     2101 tgttatcgat gaagtaataa aaattactga tgatgaagcc tttgatcata caaaacaatt
     2161 actggataaa gaaggtcttt tcacaggcat ctccactggc gctaatatag ccggtgcttt
     2221 aaagttagcc aaaagatata gcaataaaaa gattgtaacc gtctcgccgg acaacggctg
     2281 gaagtacatg tcagttcttg attatgcaaa ggaggatcat aatgattaaa tatataaagg
     2341 aacttgtgaa ttacacgtta aaaaaagacc ctgctgccaa gtctttttgg cagatcttat
     2401 tactctaccc ttctgtacac gctcagatta tgtacaaggt agcccataaa ttatataacg
     2461 gtgagagata ttttacagcc cgcttaatct cacagctggc cagattttta accggtatag
     2521 aaatccatcc gggtgctgag ttgagtaata aagtatttat tgatcatggt catggagttg
     2581 ttatcggaga aactgccgaa atcggtgaag atgtggttat ctaccacggg gtaaccttag
     2641 gcgctaccgg taaagaaaaa aatttcaaac gccacccaac gattaaaaac cacgtaatgg
     2701 taggagcagg ctctaaagtt ctcggaccta tcaccgtcca tgatggtgca aaaataggag
     2761 ccaatgctgt tgttttagat gaagtgcatc gaaatcaaac agtggtcggt atgccggcca
     2821 aagctgaaaa caagttaagg ataattaaag atgaggaata taaaaaagtg agtgccaaat
     2881 agttcgcact cacctttttt atttacatat tcaaatttga aattacttct ttcatcaagt
     2941 tagctgactt gatgaaggct tcctgttctt cttcatttaa atcgagctcc aatacatttc
     3001 tgattccttt gtcattaatt acagtcggta aacttaagct tacatcttca attccatatt
     3061 ctccctgcag caggctggaa accgttaata cagtatcttc gtcccttaaa atagcctgag
     3121 taatctgagc tactcccaga gcaaccgcat aataggttga accttttttc tctataatat
     3181 cgtaagcagc attttttacc tgttcggaaa ttctttcctt aacaacacca tcacagtttt
     3241 gatcacaaac cggacagtat ttatcgatct gggttccagc tatattggtc agactccaga
     3301 ccggtacttc actgtcaccg tgttccccga ttacataacc gtggacgttt ttagctgcaa
     3361 ttttacattg gctgctgatt aaggatctaa aacgtgcact atcaagaact gttcccgaac
     3421 ctaatactct attttttggg aaacctgata attttaaggt tacataggtt aagatatcaa
     3481 ccgggttggc tactacaagt aaaattgcat cttgattata cttggttatt ttgcctatta
     3541 tatctttgaa aatattggta tttttctgta ccaagtctaa tctggtttcg ccttcctttt
     3601 ggggggcgcc ggctgtaatg attattactt tagcatcttt tagatcacta tactcaccag
     3661 ctctaacctc aatgggttta acaaaggact gtccgtgcat gatgtccata gcttctccgg
     3721 ccgctttatc tttatttcta tcaacaagaa caatctcaga gccgatcccc tggatcatga
     3781 gggcataagc agtggtagaa cccacactac cgactcctac aattccaatc ttacctgttt
     3841 ttaattgctt attttccata atattatcac ctacaatcta tattaata
//
LOCUS       DATGCJ010000114         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_415981, whole genome shotgun sequence.
ACCESSION   DATGCJ010000114 DATGCJ010000000
VERSION     DATGCJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415981"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..2179)
                     /gene="thrS"
                     /locus_tag="VKN64_05535"
     CDS             complement(230..2179)
                     /gene="thrS"
                     /locus_tag="VKN64_05535"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMA59737.1"
                     /translation="MSAINVKLPDGSILEYEEPKTVKEVAFSIGKGLGRDAIGGVVDG
                     QEVDADYLIEDDIQLNIITIDSEEGLDIYRHTSSHVMAHAVQRLYPEAKLAIGPTIED
                     GFYYDIDMEETLNPDDLEEIEEEMVKIIEEDLEIKRVEKPKEKALKEMKEKGERYKVE
                     LIEEIEDDYVSFYTQGEFIDLCRGPHLPSTGWINKEGIKLLNTAGAYWRGDENNKMLQ
                     RIYGTSFRKEKELEKHLKQLEEAKKRDHNKLGRELDLFMTSDIVGQGLPLLKPKGAKV
                     VQLLQRFVEDEEEKRGYRMTKTPFMAKKDLYEVSGHWDHYKDDMFIIGDEDKEDEEVL
                     ALRPMTCPHQFTIYNSKLRSYRDLPIRYSETSTLFRNEASGEMHGLIRVRQFTISEGH
                     LIVRPDQLEEEFKGVINLIDYIMDTLGIREDIWFRFSKWDPSNKGKYIDNPEAWKDSQ
                     EKMKNILDKLDLDYVVADGEAAFYGPKLDIQFKNVHGKEDTIITVQIDFALPERFDMT
                     YVDKDNEKKRPYIIHRTSIGCYERTLAMLIEKYAGAFPTWLAPTQVVVLPVSDDQADY
                     AYDIKDKLFADGVRVEVDDSNEQLGYKIRQAQLEKIPYMLIVGSREAAEGTVSVRDRD
                     EGDLGAVEFTEFKDKILKEIENKVK"
     misc_binding    complement(2258..2465)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2553..3503)
                     /locus_tag="VKN64_05540"
     CDS             complement(2553..3503)
                     /locus_tag="VKN64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMA59738.1"
                     /translation="MKFKTLLFLIIFIILIPMISNIYWPSEDNLPALVFVYDDGHQED
                     IELALPVHQKYEVPAVSAVNSSTIGNVNFLDKQDLLRLQNNGWEIASHGKYHTALIYY
                     SLKEGLNKCDQKITINNSNIIEPRYSYYIYDYIQKHGEVINFKKRLKTEGDAYFKLAD
                     ELEHQYNKDSSYIMLTKKAMKKEIVESKKKLTAMGLDVNNFVYPYNGYTDPARSLVKD
                     HYIFARGGPQNGQAFLDRFINYFPLQQYNLKGISFENNEIDRQELAEILKTGAKEKSL
                     LIFYAHTGNQNFSTERLEYIIKTAQKLKYNITTFNELMRK"
     gene            complement(3558..>4059)
                     /locus_tag="VKN64_05545"
     CDS             complement(3558..>4059)
                     /locus_tag="VKN64_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMA59739.1"
                     /translation="INIPIFNIKIQGLLPSRRDELAESIAVSIEDNLLSIDNILEEFD
                     HDLIKEELNLIIEDTINKKINENFKYVMPKMLKDISREIIVDIIKEEVHNNFEDWMET
                     LGERIKEEIDIKEMIEEKIKSFSLITLEDLVLDIAERELKHIEYLGGVIGFIIGLGQL
                     ILIMFI"
BASE COUNT         1101 a          868 c          675 g         1415 t
ORIGIN      
        1 cgcaagtcgt tattattact gattttatgc acctccactt ttttttggac aataaaaaac
       61 gggtggtaga aaccacccgt ttgcatacac ataaagttag gagacataaa acgcctcaag
      121 taatacctac taactattaa ccaataatcc tattattggt gagaaacagt ggtttctact
      181 ttattgtcga attaaattgt accacaggtc gttatagtct gtcaacactc tacttcactt
      241 tattttctat ttcctttaat atcttatcct taaactcagt aaattcaacc gcaccgaggt
      301 ctccctcatc tctatctcgg actgatacag taccttcggc cgcttctcgt gaaccgacga
      361 tcaacatata gggtatcttc tctaactgag cctgtcttat cttatagccg agctgttcat
      421 tactgtcatc aacttcaacc cggaccccgt ctgcaaacag cttgtctttt atatcatagg
      481 cataatcggc ctggtcatcg gaaaccggta agacaaccac ctgagtagga gccaaccagg
      541 tcgggaaggc accggcatac ttttcgatta gcatggctaa ggttctttca taacagccga
      601 tagaagtcct atggatgata tagggtcttt tcttttcatt gtctttatct acataggtca
      661 tatcgaatct ttcaggtaga gcaaagtcga tctggactgt tatgatggta tcttctttac
      721 cgtgcacatt tttaaactgg atatccaatt tcgggccgta aaaggcggct tccccatcgg
      781 ccactacata gtctaagtcc agtttatcta agatattttt catcttctcc tgagagtctt
      841 tccaggcctc agggttatct atatacttcc ctttattaga aggatcccat ttggagaacc
      901 taaaccaaat atcttctctg atccctaagg tatccattat ataatcgata aggttaatta
      961 cacctttaaa ctcctcttct aattgatccg gtcttacaat caagtgacct tctgagatcg
     1021 taaactgtct aaccctgatc agaccatgca tctcaccgga agcttcatta cgaaagaggg
     1081 tagaagtttc ggagtacctg atcggtaggt cccggtaact tcttaacttg gaattataga
     1141 tggtaaactg gtggggacag gtcatcggcc ttaaggccag cacttcctca tcctctttat
     1201 cttcatcacc gataatgaac atgtcatctt tataatggtc ccagtgaccg gatacttcgt
     1261 agaggtcttt tttggccata aagggtgttt tggtcatcct gtaaccgcgt ttttcttctt
     1321 catcttcgac aaaacgctgc aggagctgaa ctaccttggc cccttttggt tttaagaggg
     1381 gcagtccttg acctactata tcagaggtca taaaaaggtc taattctctt cctaatttat
     1441 tgtggtctct ttttttggct tcctctaact gtttaaggtg tttttctagt tctttttctt
     1501 ttctaaaaga tgtaccgtaa attctctgca gcatcttatt attttcatca ccgcgccagt
     1561 aagcaccggc ggtatttaat aatttaatgc cctctttatt gatccagccg gtggaaggaa
     1621 ggtgtggtcc tcggcagagg tcaataaact ctccctgggt atagaaactt acatagtcat
     1681 cttcgatctc ttcgataagc tctaccttgt atctttcacc tttttccttc atctctttta
     1741 aggctttttc tttcggtttc tctactcttt taatttctag atcttcttca ataatcttga
     1801 ccatctcttc ttcaatctct tctaagtcat cgggattgag agtttcctcc atgtcgatgt
     1861 cataataaaa gccgtcctca atggttgggc cgattgccag ttttgcctca ggatagaggc
     1921 gctgtacggc atgggccatc acatgggaac tggtatgtct atatatatcc aggccttcct
     1981 ctgagtcaat tgtaattatg ttcaactgaa tatcatcttc aattaaatag tcagcatcta
     2041 cttcttgccc atcaactact ccgccaatcg catctctgcc cagacctttc ccgatagaaa
     2101 aggcgacttc tttaacggtt ttcggttctt catactctag aatacttcca tcaggcagtt
     2161 ttacattaat cgcgctcaac ttaattacct ccttattttt tctgttaaaa atctttaaaa
     2221 aatgttataa aaataaaaaa agacttaact catctactct agagacgaat taagccgcgg
     2281 ttccactcta attggttata cccactcaaa aatgttaacg gttttaaacc ggatagaagt
     2341 aattcttttt cttcctatca gctccaaggt ggtcttcagc ttattaaagg ttcagatact
     2401 ttcagcctct ggtatcattc tcttgcgaac tttaaaaagc ttactctcct cttctctgcc
     2461 tttagattaa tttttaactt ttcttaatta tattatttta gatatatgat gtcaagaaat
     2521 taacaccttt gttttattaa aaattaggag cactattttc tcattaattc attaaatgtg
     2581 gtaatattat actttagttt ttgagcggtc ttgataatat actctaatcg ttcggtactg
     2641 aaattttgat tacctgtatg ggcataaaag attaaaagcg atttttcttt agccccggtc
     2701 ttaagaattt cagctaattc ctggcggtcg atctcattat tttcaaaaga aatgcccttt
     2761 aaattatact gttgcagggg aaagtagttg ataaagcgat ccagaaaagc ttgcccgttt
     2821 tgggggccac cgcgggcaaa tatgtaatgg tctttgacaa gggagcgggc cgggtctgta
     2881 tagccgttat aggggtatac aaaattattt acatctagac ccatggctgt aagtttcttt
     2941 ttggattcta ctatctcctt tttcatcgct tttttggtta gcattatata actggagtct
     3001 ttattgtact gatgttccag ctcatctgct aatttgaagt aagcatctcc ctctgttttt
     3061 aagcgttttt tgaaattaat tacctcaccg tgtttttgaa tataatcata gatataatag
     3121 gaatacctgg gctcaataat attagagtta ttaatcgtta tcttttgatc gcacttatta
     3181 agaccttctt taagactgta gtagattaaa gcagtatggt atttaccgtg gctggctatc
     3241 tcccagccgt tattttgcag ccttaataaa tcttgcttat ctaaaaaatt tacattgcca
     3301 atcgtagaag aatttacggc tgaaacggcc ggcacctcat atttttgatg gaccggtagg
     3361 gccagttcaa tgtcttcttg atgcccatca tcatagacaa aaaccagggc cggcaggtta
     3421 tcctcactgg gccaataaat attactgatc atcggaatta agataataaa tatgattaga
     3481 aataagagag ttttaaactt catttttccc cctcaaaaga tctgtctaaa caacttttgc
     3541 ttttatacat taatacatta aataaacatg atcaatatta gctgtccgag tccgataata
     3601 aaaccgataa ctccacccag atattcaatg tgctttaact cccgctctgc tatatctaaa
     3661 actaggtcct ctaaagtgat cagggaaaat gatttaatct tttcttcaat catctctttg
     3721 atatcaatct cttctttaat cctttcccct aaggtctcca tccagtcctc gaagttatta
     3781 tggacctctt ctttaataat atcaacgatg atctcacggc tgatatcttt taacatctta
     3841 ggcattacat atttaaagtt ctcatttatc ttcttattaa tcgtatcttc gattattagg
     3901 ttcaactctt ctttgattaa atcatggtca aactcttcta agatattatc tattgataag
     3961 aggttatctt cgatcgaaac cgctatactt tcagccagtt catctcgtct cgatggtaaa
     4021 agtccctgta ttttaatatt aaatatcggt atattaatg
//
LOCUS       DATGCJ010000115        43918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_236805, whole genome shotgun sequence.
ACCESSION   DATGCJ010000115 DATGCJ010000000
VERSION     DATGCJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 43918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43918
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236805"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1253)
                     /locus_tag="VKN64_05550"
     CDS             complement(279..1253)
                     /locus_tag="VKN64_05550"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HMA59740.1"
                     /translation="MSEKETILKVENLKKYFPQRSGFFKQKVEYLKAVDDISFELKAN
                     ETLGLVGESGCGKSTTGRTILRVYEPTGGRVFFRGQDVAHVEETELQKLRKDMQMIFQ
                     DPFASLNPRMTVKNIISEGMRIHGVGNKQSRTERVEELMSIVGLRPEYMFRYPHEFSG
                     GQRQRIGVARALALDPKMIICDEPVSALDVSIQAQVINLLMDLQEEFDLSFIFIAHDL
                     SVVEHISDRVAVMYLGKIVEMTNSDKLYEEPLHPYTKGLLHSVPEPNPKVPMGEVSLK
                     GDIPSPVNPPSGCSFRTRCPHAYDRCAKEEPEFREYEEGHYAACHLLE"
     gene            complement(1253..2257)
                     /locus_tag="VKN64_05555"
     CDS             complement(1253..2257)
                     /locus_tag="VKN64_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59741.1"
                     /translation="MSSQSNQKEKLLEMKNVTTSFDTDDGKVTAIEDISFDLYKGESL
                     GILGESGCGKSVTSLSIMRLLPQDVGYVENGEIIFQGENLLDIKEEKMRSYRGNKISM
                     IFQEPMTSLNPVFKVKKQLVEVYTTHQDVNSQEAYNKALDILDLVGIPDQESVIEKYP
                     YQLSGGMRQRVMIAMALACQPALLIADEPTTALDVTIQAQILKLMKELKQEINSSIML
                     ITHDLGVIAQMVERVLVYYAGQIVERAQIDDLYENPKHPYTKGLIKSIPKIGKKINRL
                     DTIQGMVPSPFEFPQGCRFNPRCEHAMDICKEKEPPEVDVGDRHTAKCWLHVDEGESK
                     "
     gene            complement(2296..3507)
                     /locus_tag="VKN64_05560"
     CDS             complement(2296..3507)
                     /locus_tag="VKN64_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59742.1"
                     /translation="MKKNENQKEVVKKAKKSSLVKDAWKRLMRNRLAVLGMIMLAVLV
                     LVAIFAPIIAPFNPTKIYFGDEGVPPTWWGSEEGEQAQPAVSEKESTTQTGQQETADQ
                     GSWTSGEAEEESSQSQSGSDSGWSSGSEPATEESESSDSSAGGWTSSSKSSTSDEAFK
                     QETSQGQTEKTASDRQRSDNFYLLGTDQMGRDLFSRIVYGARLSLMVGFVSVGISLFV
                     GTILGLVSGFYGGKIDTLIMRFMDILLSFPYILLAIFIVAILGRGLPNAMIAIGIVGI
                     PRFARVVRGQVLSVKEEEYVMAAKTMGANNFWLISRHIFVNSLSPLIVQTTMSFATAI
                     LNAAALGFLGLGAQAPTPEWGVMLSDARSAMLTAPWIITFPGIAIIFSVLGFNLLGDG
                     LRDSLDPRQKK"
     gene            complement(3532..4533)
                     /locus_tag="VKN64_05565"
     CDS             complement(3532..4533)
                     /locus_tag="VKN64_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59743.1"
                     /translation="MLRYIIKRLIYIIPVLLIMSFLIFGMVNLIPGDPAQIMLGQHVS
                     QEALEAARERMGLNDPFLTRYGRFLTEIVRLDFGESLISKRPVSTVLKEKLPATIELS
                     TFAMIYALIVGGLAGVISAWKQNSIFDYISITIALFGISMPIYWLGFMLILFFAVFLN
                     ILPTAGRLAAEIDPSVLSSHFYLLESLFTGKWNIFISAVKHIILPGIALGTIPMAIIA
                     RMTRSSLLDVLRQDYMRTARAKGLRFRDVFIHAIKNAAIPITTVFGMQFGRLMAGAVL
                     TETVFAWPGMGKWLLDGILQRDFPVVIGATLTVTFLFVLVNLITDLTYSLLDPRIKY"
     gene            complement(4657..6237)
                     /locus_tag="VKN64_05570"
     CDS             complement(4657..6237)
                     /locus_tag="VKN64_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59744.1"
                     /translation="MSRRTLLVLGLVAALVVGLFGTALAQEPQKGGTLVFGRGSDAVR
                     LDPADVTDGESVKVITNVFEGLVKYKEGSTEVKPALATQWRVSEEGKVWEFKLREGVT
                     FHDGSEFTADDVVFTFRRQMNEDHPYHKGDFAYWGYMYNMVESVEKINDYQVKITLSR
                     PYAPFIYNLATFPAFIVSEEAMKEKGVEGFRTSPVGTGPFEFVNWEHGSTITLERFEN
                     YWGENAYLDKVVFSVVPENTTRVMGLKGGDLDMIDGLGPQNIESVRSDDNLKLLTQPG
                     MNVGYLAMNNLKEPFDNKLVRQAINLAVDKEAIIEGAYKGTAEPAKNPLPPTLWGYND
                     EVEDYGYNPEKAKELLEKAGYPDGFSAELWYMSNPRPYMPNGKKVAQAVQSNLADIGV
                     DIELVTYDWGTYLEKTENAEHDMALLGWIGDNGDPDNFIYVLLDKDNTKKGSAGNIAF
                     YRSDELHNILIQAQQTMGDQRLRAGMYKAAQHVIHEDAPWVPLSHVMKAMGTAQDVNG
                     FVIHPTGGVYLQNTWIEQ"
     gene            complement(6452..6823)
                     /locus_tag="VKN64_05575"
     CDS             complement(6452..6823)
                     /locus_tag="VKN64_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMA59745.1"
                     /translation="MATYQYEFKKKRKGLLAKLLITMVALFITAYIIPGMSIQGLFAG
                     FVAALILGIVNVVVKPIFIVLTLPLTILTMGLFLLVVNGLMLMLSASLVPGFMVAGFW
                     SAVFGAILLSIVTWFLNSILD"
     gene            complement(6834..7916)
                     /locus_tag="VKN64_05580"
     CDS             complement(6834..7916)
                     /locus_tag="VKN64_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HMA59746.1"
                     /translation="MKTVLIMAGGVGTRFWPVSRMDNPKQFLKLTSDEKTMLQCTVDR
                     VREMVDIDRVFIATNDRYVDKIAKQLPDLPRENIIVEPCKRNTAPCIGLASIYINRKY
                     PESTMVVLPSDHVIKDNDNFIDILDSAADYAKQGENIVTLGIKPNRPETGYGYIHRGE
                     EVHNIKGNKIFKVKEFTEKPDYEKANDFFESGNYYWNSGMFVWKTKTILNKIKEHLPE
                     IYETLSKIAKAIGTDNEEDVIDEQFRCITGISIDYGVLEKENEIFVIPADFGWNDVGS
                     WTALGEVGKKDQDNNIVNANSEHVSIDTEDTIIYSEDKLVTSIGVDNLIIVNTEDAVL
                     VCNKDNAQDVKKLRKLLKDKGLDKYL"
     gene            8212..9063
                     /locus_tag="VKN64_05585"
     CDS             8212..9063
                     /locus_tag="VKN64_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59747.1"
                     /translation="MKKLLILLFAFILLFIPVRTNAYEVDFNLESKLGDLVVTNTPPV
                     GNIEENNLSLNTIMLKANFNQYIFREWADFYSISLGTNLLDTSTEDTEIIYYEESLYN
                     KVDKLKGNTELNSFYFDLLLANYLLDYSTPRESYLILIGYEYDNFNYETKENIKIASH
                     DIQTHIPYIGFIYNRNFTRNIKNRFIFKFSPYTYTNNSSKNYNNTYINDSTSDGNYFS
                     LKNNVRYKINKDMYFTGGGQFKVLKTTGSGTRYYYSGVNEGISYNINSETEVEEYSLN
                     IGIQYLF"
     gene            9328..9471
                     /locus_tag="VKN64_05590"
     CDS             9328..9471
                     /locus_tag="VKN64_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59748.1"
                     /translation="MNFGKKIKCKVCGTRNWSGNEYCEKCSAKLDYNNIENIQEKVRK
                     KIA"
     gene            9650..10798
                     /locus_tag="VKN64_05595"
     CDS             9650..10798
                     /locus_tag="VKN64_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMA59749.1"
                     /translation="MDNIYLDNAATTKVIPEVLEKICEVLETNFANPSSLHGLGIDSE
                     KKIKNARSKIANALNIKPAELIFTGSGTIANNIAIQGTVNRLKKFGKKIITSKIEHKS
                     VLEIFKHFEKEGFEVIYLPVDQNGMIDLDKLKETIDKETILVSIMAVNNELGSYQPLK
                     EISNIINKYDNLYFHVDGVQALGKVSLDLKGLNIDLFTVSSHKVHGPKGVGALYLNND
                     IIIDKILFGSSQEKGFYPGTENTAGIAGFGKAVEHLPTQPEIKQIKSLRNMLIDKIKA
                     EIPDIIINTPSGENAAPHILNVSFKNIKGEVLVHSLEKDGIFVSTGSACTSRKDSISH
                     VIQALNLPEGYRDGSIRFSFSQYNTKQEVEYTFKSIKENVANLRKIMG"
     gene            10803..11987
                     /gene="thiI"
                     /locus_tag="VKN64_05600"
     CDS             10803..11987
                     /gene="thiI"
                     /locus_tag="VKN64_05600"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HMA59750.1"
                     /translation="MSNLYLIRYGEIGLKGANRNIFEDKLMSNIKKAVEWEDEDLNIY
                     KTPGRIFLETSADKKIVKERLLKIAGLVSFSPVKKLPLDYAQIKKEGLAAAEKFIADK
                     DRITFRISARRSNKEFEYDSMELQYDLGEYVLENIGEDKIEVDLHEPDLDINVEIRKK
                     HAYVYSETFEGMGGLPVGTTGNVGLMLSGGIDSPVAGYMGLKRGVRLIPVYFHSFPFT
                     SDRAKEKVIDLAEVLGQYQNDIDLHVVNFTEILKEINEKCPEKLITIIMRRLMIKITE
                     KIIEKQNGKAIITGESIGQVASQTLEAIKVTNHGPDLPILRPLLGLNKVQIIKLAKEI
                     GTYDISIQPYEDCCTVFVPDSPETRPTLQQIKEGEADLHVEDLIEEAVANVEVINIQP
                     YS"
     gene            complement(11991..12788)
                     /locus_tag="VKN64_05605"
     CDS             complement(11991..12788)
                     /locus_tag="VKN64_05605"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HMA59751.1"
                     /translation="MIDIHNHLLPEVDDGAKSQSETLEIIKTAEEYEIHKIVATPHYM
                     EESYQIEGETIKKKVRELNELLNKKDINVEILAGHEIYITPDLIKKFKNKDILSINNS
                     KYLLLEMPMNYIPEYLDQVFYDLSVLGYKPVIAHPERNEEVIKNPDILYEWVKDGIIF
                     QLNAGSLFGVYGNNVKKTAFELVDNYLVQVIASDCHGSSKVNLEWLARAKRKLEDICG
                     SYTVQFFTNAEYIINDEEIIYEEPTHFKSKSIWDIFNKINVFSGNEK"
     gene            complement(12804..14084)
                     /gene="purD"
                     /locus_tag="VKN64_05610"
     CDS             complement(12804..14084)
                     /gene="purD"
                     /locus_tag="VKN64_05610"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMA59752.1"
                     /translation="MKVLVVGNGGREHALIWKIYQSEKVDKIYVAPGNDGMTDLAERV
                     DIAASDITALADWAYEHKIDLTVVGPEKPLVKGIVNEFEKRGLRIFGPNKLAARVEGS
                     KVFAKNILEKHNIPTAEFEVFTEPAEAMEFLKEADYPLVIKAEGLAFGKGVVIATNKS
                     QAEEAITRIMEDKVFGDAGSRIVIENFLEGEEISVMVFTDGKNCVPLEFAQDHKAVFD
                     GDEGPNTGGMGSYSPLPFVNEKLKKRIHNEILKPALEAFNEEGLNYKGILYINMILTD
                     MGPEVLDFNARFGDPESQVILPRMNNDIIDIFNKTIDEELEDVEIEWDTRAAVCVILT
                     SAGYPLTFDTGKEIKGLDQINRYDNLLVFHAGTTKIGDRYYTAGGRVLGLTVLGDNLD
                     DAISLIYEYIDEVYFEDMHYRTDIGVSRRESINY"
     gene            complement(14149..15411)
                     /locus_tag="VKN64_05615"
     CDS             complement(14149..15411)
                     /locus_tag="VKN64_05615"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMA59753.1"
                     /translation="MAVKVVIGAQWGDEGKGKVTDKLAETADYVVRFSGGNNAGHTVI
                     VGDEKYELHLIPSGILYPEKKSVIGNGVVIDPVALNEEIAGLKERGITFDNFHISEQA
                     HVVMPYHKTFDGLEEERKGDNKIGTTKKGIGPTYTDKAARRGIRICDLTDEEVFKEKL
                     KNILEYKNLIISKVYDKPKFNYEEILEQYLEAAKKIKPYVTDTSLLLDEAYKKDKNIF
                     FEGAQGTLLDIDYGTYPYVTSSNPTSGGVCTGAGFGPTKIDDIIGVAKAYLTRVGAGP
                     FPTELFNENGTFLLEKGHEYGVTTGRPRRCGWLDLPLLKYAVRVNGLTELVLTKVDVL
                     SGLEQIKVCTSYKEGDCTISQAGLKGERAPQYESFAGWEKDISGLTDFDKLPKEAKNY
                     INMIEKEIEIPITVIGTGPERDDAIIRK"
     gene            complement(15430..16725)
                     /gene="purB"
                     /locus_tag="VKN64_05620"
     CDS             complement(15430..16725)
                     /gene="purB"
                     /locus_tag="VKN64_05620"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMA59754.1"
                     /translation="MLDRYSRAEMKNIFSEENKFQKWLDVELAVVKARQKLGEVPEEA
                     YQKIADNASFEVARIKEIEKETRHDMLAFIETVNENLGNEAKYIHEGITSNDIKDTGL
                     ALQLSEALDLILKDLRKLKRSIKEKALLHKKTVMIGRTHGIHAEPITFGLKLAVWFKE
                     IERHEKRFNQLAEVIKVGQISGAVGTYATINPEVEKLVMQELNLDTAMITNQILQRDR
                     HADLMSKLSLAAASLEKFATEIRNLQRTDILEVEENFSKGQKGSSAMPHKKNPIVTER
                     ISGLARVIRGNLIPAFENVNLWHERDLTHSSVERIILPDSFILLDYMINKFKDVLDNL
                     KVNEETMQINMNKTNGLIYSQKVMLALIDKGLKRERAYELVQRNALKAWENNESFQKK
                     IENDGEVMTYLNKEELNKIFDYENFIKEVDYIFERGKLK"
     gene            16889..17926
                     /locus_tag="VKN64_05625"
     CDS             16889..17926
                     /locus_tag="VKN64_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59755.1"
                     /translation="MENIKLIDHKKIRICCDHEQQSIELGRESINHGFLKTLPGPSLP
                     VFLYLITHLDNNNSIKTNPALMAGFLALDREQIKEGLKILENKDYITLKEKKEGNYTY
                     KIKLNLDKIVGSCYCESDESQDEDLKEYRQEVINHNNLKEEAYKKALISFLPKGVEHS
                     LIEEEIEHWLNDFDKDMIRELIRRVDKWINNNNKSSKEGFYYLHGIIDDWYNKEIFDY
                     DRLKYFDRMYRETNELAKCYGLKNWYNVSESQMEIFYDWLNDNNGINLSVAKFAIKEA
                     FKRKSDGQPSLKYIEDNFISPLKENNIRNINQAKKVLNNNSFQAEQTDQTNKGSETSW
                     ETFEWDIEDLN"
     gene            17948..19330
                     /locus_tag="VKN64_05630"
     CDS             17948..19330
                     /locus_tag="VKN64_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB family ATPase"
                     /protein_id="HMA59756.1"
                     /translation="MALEQNYPEQLEDNVEQEFQLLSILLQYPDKIDEVADILETRHF
                     LNPKAQLIYDILLKQFERDNQISKTKLFLKLKNENIVKRPEEMIENLTSGFNTPEELK
                     PTIDIIKKNHQKDLLIRASKKIDKLATQTELDISEYQSRAQEIIFTATNESSDLDKHI
                     YTMEEALLESFEAYLNRKEKKVDVGLQTGFMSLDNLIGGFKKGHLNVIAASTSMGKTA
                     FAINIAKNVLKRNEPTAIISLEMEAPEIVDRMIIQEAQVNGWKYNQGETTDEEDKRIS
                     KALDDLHEFPLAISDERGLNVAQIRARLRKFKAQLDELSLVIIDYLQMIQLPEEHAQN
                     TARAVGEIVLQLRNLASELEVPIVLISQISRSFTSRQDKRPVLSDLRDSGNIEEVADG
                     VIFLYRHAHTSAAAREKAEAEGTEQDTEIIIAKQRTGQTGSVKLLFEEDYISFVDPEN
                     VSFEGMIPNE"
     gene            19323..19496
                     /locus_tag="VKN64_05635"
     CDS             19323..19496
                     /locus_tag="VKN64_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59757.1"
                     /translation="MSKDLDLKIIYEFTDKYEQLKKEGKISEKQFNEILSLLDNLEKF
                     SKEELDDKLEDIT"
     gene            complement(19479..20573)
                     /locus_tag="VKN64_05640"
     CDS             complement(19479..20573)
                     /locus_tag="VKN64_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59758.1"
                     /translation="MLTKKSLIFILTITLLFTTTTALASSYQFGGEVSTGLNSVFFDE
                     TNINSIYETINLNLDFNNANFEITGSNNNGKQEFKINLKKAYVRHKFKGTRVTLGRQP
                     ISWSFGSLINPIDYSLGAEALDEETSAKYVDAVEFHYPINWYSNVSFVTEFNNSYDKY
                     GFRARTLIKDYDVSFNYVTQDAVESDINRWGLSLKGDIKNAGVYGSYTNINYKTPMVS
                     DVNTQAFMVGTDYSYFLNQGFGNRLYMQGEYHLVEKNEGLAVLIESLAGAGIGLDYRK
                     FLGGDEYLDIFLTNVNYSINDFSSIGFFTLTSLEDGSTALIPNYSNQLSTNTTLNINV
                     SYLSGSETSDFGAGRGLPKAALNIELSYVF"
     gene            complement(20594..21334)
                     /locus_tag="VKN64_05645"
     CDS             complement(20594..21334)
                     /locus_tag="VKN64_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HMA59759.1"
                     /translation="MKFKKVTALIILSLFIFSSSALAITAEEIINKRDDNEYLKQARV
                     ESTMIINNAGRELEKKMVSLIEGDKALVRFTNPMDRGTKFLKREDNLWMFFPDAEDVV
                     KLSGHMLERGMMGSDFSYKDMMESEKLTELYNFELVGEVEYNGRPCYKIEAIKEEGAE
                     VSYYKRIGWIDKERFIGLREELYAESGKLLKVAEVTEVEEIEGRWYPIRTIMENKLRE
                     NTSTTFIIDSIEFNPEIPEGTFSLESLQ"
     gene            complement(21350..22609)
                     /locus_tag="VKN64_05650"
     CDS             complement(21350..22609)
                     /locus_tag="VKN64_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMA59760.1"
                     /translation="MKFLITLAGRNLFRNKMRTIVSITAIAIAVMVVIFARGLVMGVL
                     NSSFSLYVQYDSGHIRIIDEKYDQKEKLLSLIHPVDGFNSKGIMPMVEKLEAEEGVEL
                     AIPRLRFGAMASPDEEIVRMLGWGVLPEKEIEFTEIESKLVEGRMIEMGQREVVMGTG
                     LMDSLNKNVGDKVTILYKNSFDAFKGSTFKIVGKVDHYLPLINDRLFYLPIDQAQRIL
                     LMQDMATEILLKTKSMSAAQSIEPQIEQLIQSNDGRGKYAVQTWGEANPLLKLFDVAK
                     YIYNVIYILLLLLAAIVVINTMVMIVKERTQEIGMMQALGLKSREILTMFMIEGTFMG
                     LVGSLIGSVSGGALTKYLAVHGIDYTQAVEGVIDEVFMNPMIYPVYNLENVIFGFILG
                     TIVTALTCIIPARRAANLEPNDALRDI"
     gene            complement(22625..23866)
                     /locus_tag="VKN64_05655"
     CDS             complement(22625..23866)
                     /locus_tag="VKN64_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMA59761.1"
                     /translation="MFLTKLAIKNLARHKKRTAITALVISFGIFIFLLSDSLMIGLRD
                     ISLDNNIELETAHLQIMEEDYWDEKEEAPLDYLLNENNEVINQIDNKYSNIAQHTARL
                     NFSATINNGQEEYPIQVIGIQPKKEIDIFELEEYILKGEMLSSGQKAMIGKSLANMMG
                     FEVGDYVTLLFKTEEGTFNTIDAELQGVLDTPNPMINEMYVYIPLSLAQDSLNLENKV
                     SKYLFRLENRDQSVEMAANLNEDFKQAGSSIKAYNWEQLSKEVLAMVRQGNIENLVML
                     AIIILLATVGVINTVILAALERLEEIGMMKALGLKAREIIYIFVIESAGIGVIGSTIG
                     IIFGAIGVYLLKNIGISISAMTGGTTSFGMPIAGKIYGGWHPEAFLFIFIFGIIISTL
                     ASILPARWGAKKDPVKAIYHR"
     gene            complement(23853..24563)
                     /locus_tag="VKN64_05660"
     CDS             complement(23853..24563)
                     /locus_tag="VKN64_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59762.1"
                     /translation="MPLLKLENVKKTYHQGKIDVPAIRGISIEIEKGEFTTIFGPSGS
                     GKTTLLNIIGCLDKPSAGEIHYNNTKKLSELSKNELSDFRRYNLGFIFQGHNLIPVLT
                     AFENVEFAIRIIDEMTKKERREKVLDILDSVGLEGLEDRRPNELSGGQKQRVAIARAL
                     VKNPKLVLADEPTANLDSETSEEVLDIMLKMNQKLGTTFIFSTHDPQVMKYARRLIEI
                     RDGKVTTDKKGEDLDVPH"
     gene            complement(24567..25193)
                     /locus_tag="VKN64_05665"
     CDS             complement(24567..25193)
                     /locus_tag="VKN64_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA59763.1"
                     /translation="MPTTTFFNLPEEKKKRVINAAVDEFAQNGYTKSSITRMVNKAQI
                     AKGSFYQYFEDKEDLYRHILKKAVEKKFKYVQKELNDYEGDDFFEYWRRLNIAGLKYA
                     EDNIKLAEVASDIVKNKDQKFYRSIIDEYKNLGEGMFERLLKKAVEAGQVRKDINITY
                     TAGLLYRASFFITDYFFEKKDIKQLDEFIPLIDNLIEIIQYGIKKEGG"
     gene            complement(25506..25976)
                     /gene="rplI"
                     /locus_tag="VKN64_05670"
     CDS             complement(25506..25976)
                     /gene="rplI"
                     /locus_tag="VKN64_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMA59764.1"
                     /translation="MEIILREDVDKLGASGDIVEVADGYARNYLIPRGYAVRATESEK
                     KRLKHEIERAKKRKAERTEEAQELKAKLEDERFIFPVKAGESGKLFGSVTTKDIAEAA
                     EEEGYEIEKRNISLDENIKELGVHKVPVKLFDDIYANLIVEVVEAQEDEEEKEE"
     gene            complement(26001..26891)
                     /locus_tag="VKN64_05675"
     CDS             complement(26001..26891)
                     /locus_tag="VKN64_05675"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HMA59765.1"
                     /translation="MLNQKIKDGIKAVIYLLVLTLINIYIPFLSVFVLVVWPIPVAVI
                     TLKYDLNTTAAVIAIMAILSGLLFGPMLGLMAVIGYGLVGFVIGNCIKEGIKPLRTLL
                     FTIGAVLVSHLLLLYVSRYMLGIDFQSIINEVTRIIGQTGNMSGMEDMISSQIQLLQR
                     IFPALIGISAIITGSLNYYVTNWYIRKTGYKRTTYKNIKYWFLPRWFISLGIVVSLIL
                     QRNIFFVNLNMLLLFLALIQGYAVAIYFIDKKTDSSLFKVIFTAVVLFIPIFPPAVIL
                     LGLVDFWFDFRKLKNNKKQA"
     gene            complement(26982..27194)
                     /gene="rpsR"
                     /locus_tag="VKN64_05680"
     CDS             complement(26982..27194)
                     /gene="rpsR"
                     /locus_tag="VKN64_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMA59766.1"
                     /translation="MGRGRNRSCFFCAKDKEIDYKDLKTLNRYITDRGKIVPRRITGN
                     CSKHQRDITKAIKRARKLALLPYVKE"
     gene            complement(27210..27650)
                     /gene="ssb"
                     /locus_tag="VKN64_05685"
     CDS             complement(27210..27650)
                     /gene="ssb"
                     /locus_tag="VKN64_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMA59767.1"
                     /translation="MLNHIVLIGRLVRDPELRYTSNGTPVANFTLAVERNYRNQQGET
                     EVDYIKIVTWRKLAENVAHHLGKGRLVAVEGSLQIRKSQGKNDNRTYINPEVIANNVK
                     FLDWPSDNNKGKSSRSDYQKQEPEQEDNNMDDQDLSDDDFEVPF"
     gene            complement(27668..27964)
                     /gene="rpsF"
                     /locus_tag="VKN64_05690"
     CDS             complement(27668..27964)
                     /gene="rpsF"
                     /locus_tag="VKN64_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMA59768.1"
                     /translation="MDKVRNYETAIVLRPDLDEEKREAIIERIKSVITDNDGEINNID
                     EWGNRQLAYEIEDYRTGYYAFINFNSSPDVLDQLEHNYRILGEIIRSLIIRKDD"
     gene            complement(28110..29204)
                     /gene="ychF"
                     /locus_tag="VKN64_05695"
     CDS             complement(28110..29204)
                     /gene="ychF"
                     /locus_tag="VKN64_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMA59769.1"
                     /translation="MEIGIVGLPNVGKSTLFNALTKAGVSAENYPFCTIEPNVGVVEV
                     PDKRLDDLYELFSPPKKIPATIKFVDIAGLVEGANKGEGLGNKFLSHIREVDAIAQVV
                     RCFEDENVTHVDKDLNPVKDIEVINTELIMADLETVEKRKEKTERMLKSGEDKYKKEY
                     EILEKVEKGLNEGIPARNMELDQKERELVDELFLLSDKKVIYIANISESDINKEDNEY
                     LTRIREYAKEDNTEVVPVSAKIEADIAELEDEEEAQLFLDDLGLEESGLNKVIKSCYR
                     LLDLITFFTAVQKEIRAWTVKKGTKAPQAAGKIHTDMERGFIKAEVISYEKLMEAGSI
                     KNAREEGLLRIEGHDYIIQDGDFCYFKFNV"
     gene            complement(29214..29393)
                     /locus_tag="VKN64_05700"
     CDS             complement(29214..29393)
                     /locus_tag="VKN64_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HMA59770.1"
                     /translation="MADLKVGDRVKMKKPHPCGSNSWKVIRVGMDIKIECENCNHIVM
                     MPRRKFEKNLKEIIK"
     gene            complement(29409..29951)
                     /locus_tag="VKN64_05705"
     CDS             complement(29409..29951)
                     /locus_tag="VKN64_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMA59771.1"
                     /translation="MLEDEYINRLDDELQKKYKEASDLLDIGEFEEAKERFNEILEVE
                     EKFLPILNKLAVIEIYNDNLDKAKEILNEILQEDPEYVPAITNLGSIAKKKGDLDKAE
                     RFYEKAIEIDPDYGNAYNNLGVIYREQGKYTKSVRYLKKARKRGSYSFKFEDKPLYKN
                     KGCLFFVGVIILAILYFVLT"
     gene            complement(30057..31499)
                     /gene="gcvPB"
                     /locus_tag="VKN64_05710"
     CDS             complement(30057..31499)
                     /gene="gcvPB"
                     /locus_tag="VKN64_05710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HMA59772.1"
                     /translation="MAEKLIKDYSSPGRKGYSLPPLEVPELDLEEQLGSDYIRDELDF
                     PEVSEVDVVRHYTALSELNYGVDSGIYPLGSCTMKYNPKRNEDIARISKLTNLHPYQA
                     QEDVQGSLEILYQLKQDLAEISGMDEMTLQPASGAHGEFTGLLIIRKYFEDKGIDKSK
                     VIVPDSAHGTNPASATMAGFDIVEIDSNEEGMVDIEKLKAAVDDDVAALMLTNPNTLG
                     IFEKDIQQIADIIHGVGGLLYYDGANLNAVMGYARPGDMDFDVLHLNLHKTFSTPHGG
                     GGPGSGTVGVKEFLKPYLPRPVLQKKEGKYCWDYDRPKSIGKIHGFYGNYGVLIRAYA
                     YIKTLGGSGLKKTTENAVLNANYMKSQLKEEFLLPYEANSLHEFVLSGIKQKEKGGDT
                     LHIAKRLLDYGQYAPTIYFPLVVKEAMMIEPTETENLETLNRFIDTLKTISKEVEENP
                     EVVKNAPHNTSVRKLDEVKAAREPDLGWQN"
     gene            complement(31500..32840)
                     /gene="gcvPA"
                     /locus_tag="VKN64_05715"
     CDS             complement(31500..32840)
                     /gene="gcvPA"
                     /locus_tag="VKN64_05715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HMA59773.1"
                     /translation="MDYVSNTTQEREYMLKEIGVEAVEEIFDSIPKEIKLERELDVAE
                     GMSELELKRHMHEISEVNNSLENYTSFLGAGAYDHYIPGIIDHLISRQEFYTAYTPYQ
                     AELSQGTLQVIYEYQSMISELTGMGVANASLLDGGSAMGEAVLMASRISRNKKILISS
                     AVHPSYKEVAKTYGEPQNLEFVEIGLDGSITDLTELESNLDDDTAVIVVQYPNFYGSI
                     EEMKKIQELKEQYKRVLLLVVANPIALGTLTPPGAFDADIVVGEGQSLGNTLNYGGPY
                     LGFLACKDDRKFIRQTPGRIAGATDDAEGNRGYVLTLQTREQHIRREKATSNMCTNEA
                     LNAAISTIYMSTMGRQGLKEVAEQSFTKAHYLAEKIDELDKFEVVNIDNYFHEFWLKT
                     KLPVSKVLTELKENKILGGIDLSRFDEEEGILMSVTEKRTKDELDELVQLLGAM"
     gene            complement(32844..33227)
                     /gene="gcvH"
                     /locus_tag="VKN64_05720"
     CDS             complement(32844..33227)
                     /gene="gcvH"
                     /locus_tag="VKN64_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMA59774.1"
                     /translation="MSIPNDLFYTENHEWISVDGKDAVVGITDHAQEELGDIVFVELP
                     SVEEEVDQFEEFGVIESVKAVSDVFMPASGVIVEVNEELMDRPELINEDPYGSGWLIK
                     IEIKDKDELEELMDSTEYEAFLEEE"
     gene            complement(33246..34325)
                     /gene="gcvT"
                     /locus_tag="VKN64_05725"
     CDS             complement(33246..34325)
                     /gene="gcvT"
                     /locus_tag="VKN64_05725"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMA59775.1"
                     /translation="MKSTALYEEHQSLDAKMIDFGGWQMPVQYSGIIDEHKAVRNKAG
                     IFDVSHMGEILISGKEALDLVQKIITNDASKLTNGDVLYTPMCYQDGGIVDDLLVYRI
                     DVDKFLLVVNASNTTKDFRWVEKNAELFEQAEVEDKSDYYGLIALQGPISREIIQPLL
                     EEDISNLKYYTFIETDIAGVEAIVSRTGYTGELGFEVYLKAEDAVQVWNAMLKEGSDK
                     GLKPAGLGARDTLRLEKALCLYGNDIDETTNPLEANLGWTVKFDKEDFNGKEALEKVK
                     EDGVKRTLVGFIMQERGIPRHGYEIKVDGEVVGEVTSGSYSPTLDENIGLAYVDKKLA
                     EEDTELDVCIRKREVKAKIFKTPFI"
     regulatory      complement(34409..34504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(34543..34983)
                     /locus_tag="VKN64_05730"
     CDS             complement(34543..34983)
                     /locus_tag="VKN64_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMA59776.1"
                     /translation="MFNKEEDVKRDEISTIFGQQTEFDGKLKTKGSIRVEGSLTGTLE
                     ADGDVFVGESGEVNSNIEARKVVIAGKVEGNIIAKDKLEILETGTLEGDIKAKKLVVE
                     EGAKFIGKSAPLENSTKKQSFTQSKEDKNKKKNEKNVEKKSKEV"
     gene            complement(34989..36020)
                     /locus_tag="VKN64_05735"
     CDS             complement(34989..36020)
                     /locus_tag="VKN64_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMA59777.1"
                     /translation="MNGSLWKNIKEFLSEKLYITVTPSSTRNKIKNFSFRRVIPIIII
                     LLLVIGIGILSCNYLYYKGKYKQTQANFQEFKNSVNIEELQEENKRLKKNLLVLSQNT
                     EELMASLRSIKEANQEIRYILGEDTNINEDNDFDEEINITSILRTNSNILKQGIPMGG
                     SELNLYYHETDKLIEQMKTEINIAEDTISKQRSVQNELKDEAVEYKDMRSSLPNLWPL
                     ADSGNCYISSNFGWRIDPISNQQEYHEGLDIAVWYNTPVVATAPGKVTFVGWNGGYGL
                     SVKVEHGHGYSTYYAHLNKSKVSKGDKVTEGEVIALTGNSGKSTGPHLHYEVRINGVP
                     KNPRKFIGR"
     gene            complement(36114..36800)
                     /locus_tag="VKN64_05740"
     CDS             complement(36114..36800)
                     /locus_tag="VKN64_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HMA59778.1"
                     /translation="MIGTIVNVIAILIGSLVGFLVKGRLPERFQNIVIQGISLAVLII
                     GLQMAFSVESTKGTLVVIFSLVIGGIIGEILKVESRLEGFGEKIQERFGSDDGLFVQG
                     FVQSSLIYCVGAMAIMGAIQDGLNNDPSILFTKSLLDGTASVAFSATFGIGVMFSSIP
                     VLLYQGSISLLASWAQKLLNQDVVAMMTATGGLLIMAIGLNMLLSAEIKIGNLLPSIF
                     VAIVIAQFVF"
     gene            complement(36806..37465)
                     /locus_tag="VKN64_05745"
     CDS             complement(36806..37465)
                     /locus_tag="VKN64_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HMA59779.1"
                     /translation="MFIIVVGGGKVGSYLIKELLEKDHKVHTVENNTDNCKRLKQKYG
                     VKVFCGDGTNENLLEEAGINEADVVLAVTGDDQDNLVICQMAERKFDVPRTFTRVNTP
                     GNERLFDWLGVNVAVSSASILTALVDQEMTLQDLRYILNKNHDKIELVRIPVGDNCDY
                     LNKKLREIDFPLESVLVTILRGDESLVPRGDSIIKKDDLIVALTRREYKEELIKMFNG
                     G"
     gene            complement(37513..38361)
                     /locus_tag="VKN64_05750"
     CDS             complement(37513..38361)
                     /locus_tag="VKN64_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMA59780.1"
                     /translation="MNKKRLGKGLDALLSKSQGEKREHEIKDIDIEKIKPNPYQPRDR
                     FTEESLDELANSIANKGVLQPITVREVQYDNFELVTGERRWRAAKKAGLDTIPAIIRE
                     FKDDEMMEVALIENLQREDLNPIEEAYAYQKMIEEFDITQKEVAERVSKSRSAIANTL
                     RLLNLPPSVQEIVSRETISMGHARALLPLESKKQVDLANKIVEEDLSVRKTEELVKKY
                     QNKDKKEKTKKNKKELAPKWVKARELLAERLGLDVDIKDKVSKKVVTINCKDYKEMEK
                     IIDKLS"
     gene            complement(38354..39115)
                     /locus_tag="VKN64_05755"
     CDS             complement(38354..39115)
                     /locus_tag="VKN64_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA59781.1"
                     /translation="MARKIAIVNQKGGVGKSTTAINLGASIAERGREVLIVDVDPQGN
                     ATSGLGLEKSDLEYSIYDALINDTDLNDIIQPTGHHNFYILPANIDLAGAEIELVSVM
                     SRESRLKKKIEESDIEYDYIIFDCPPSLGLLTLNALTASDSLIVPIQCEYYALEGLGQ
                     LMQTIELVQSNLNPDLTLLGVVLTMNDARTNLSSQVIEEVKKYFGKDVFDTIIPRNVR
                     LSEAPSFGEPIITYDPSSRGAHAYKELPKDVIARE"
     gene            complement(39129..39947)
                     /locus_tag="VKN64_05760"
     CDS             complement(39129..39947)
                     /locus_tag="VKN64_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMA59782.1"
                     /translation="MDFPFLGKKSQNNKIEKIKTDKIVPNPHQPRSEFSEKNLQKLAE
                     SIENFGIIQPLIVSEKDGKYQLIAGERRLRAAKLIDLEQVPAIVNNYKEEEVAEISLV
                     ENIQRQDLNFLDEALAYYSIMNKFDLSQTEMADKVGKSQGTIANKLRILKLPEEILAL
                     IRKYNLSERHARALLKVDDNEKQKEFIKEAKEKDLSVRGLNRKIDKYLKKQKEPDSKN
                     VKTYYADMRLLKNTLNSAIDQLRKAGIEVKVDKQDDKEYIRYNIEIPKKQEENK"
     gene            complement(40002..40724)
                     /gene="rsmG"
                     /locus_tag="VKN64_05765"
     CDS             complement(40002..40724)
                     /gene="rsmG"
                     /locus_tag="VKN64_05765"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMA59783.1"
                     /translation="MNKNSLKDYLDRGFKQIGLELNKNQKEQILKYIDFLIQENKKYN
                     LTSITEPKEIVKVHFLDSAGLFLKSDIGGKVIDIGTGAGFPGFVFKILKPDVKLTLLE
                     ASLKKANFLKMLQVELELYEDIEVLHARAEDLGQNKDYRFQYDYVTSRAVAPLNILLE
                     YTAPFCKNEGKILLYKGPSYQDELKEAHKAIQKLNLKYMNSYKLDIPFLDGTRYILEF
                     VRKNELSDKYPRRAGIPKKRPL"
     gene            complement(40717..42642)
                     /gene="mnmG"
                     /locus_tag="VKN64_05770"
     CDS             complement(40717..42642)
                     /gene="mnmG"
                     /locus_tag="VKN64_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMA59784.1"
                     /translation="MENNYNYNKVFEKEYDIIVIGAGHAGCEASLAPARMGFDVLTMT
                     VNLDHVAFMPCNPSLGGPGKGHIVREIDALGGEMAINMDETMLQIRMLNTAKGPAVHG
                     LRGQADKKKYHTRMKQVLEEQEHLDLKQEIAETLVVEEGEIKGVISKTGILFKTKKVV
                     MTTGTFLKGKVIIGEATFNSGPNQQYPANKLSESLKDLGFNLRRFKTGTPPRVNKRSI
                     DFSKMHEQTGDEGLSFSFDSPPLERDQVSCYLTYTNEETHKIIRDNKMRTPLFSGVIE
                     GVGPRYCPSIEDKVVRFPDKDRHQLFLEPEGELTGEYYISGLPTSLPEDVQYEILKTI
                     PGLEKAEIMRAAYAIEYDCLDPSELQLSLETKRINGLYSAGQINGTSGYEEAGGQGII
                     AGINAALSLKGEEPLILDRSQAYIGVLIDDLVTKGTEEPYRIMTSRAEYRLILRQDNA
                     DLRLKEIGYNLGLISEERYQKFLNKKHDIERTLEFLRDVQVTPTPENRERLKSLDSGA
                     LKKPVKLAKLLKRPEVEYDDLKFFAPNLPEVSQEIKEQVTIQTKYKGYIDRQESQIEQ
                     FKKMEDKLIPEDINYDELENLRLEAREKLDKIRPRSLGQASRISGVSPADISVLMIYI
                     EQQEREQKSNESEQENE"
     gene            complement(42663..>43918)
                     /gene="mnmE"
                     /locus_tag="VKN64_05775"
     CDS             complement(42663..>43918)
                     /gene="mnmE"
                     /locus_tag="VKN64_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMA59785.1"
                     /translation="LTKADSHTAHHGYVVDPATKDTIDEVLAIIMVHPKSFTGENTVE
                     FDCHGGMVPLESVLELVLDQGARMAEPGEFSRRAFMNGKLDMAQAEAIIDVINSKTEK
                     SLSIAMEQLKGGLSEKVNDIKDEVITLLAHLEASIDFPEDEIEGFDSSQLEDKVKNIE
                     KPIEKLIKTAKQGKLYQEGVKTVIVGKPNVGKSSLLNYFLQENRAIVTDVPGTTRDVI
                     EEYINLDGIPLKIIDTAGVRESDDVVEQIGVEKTRNSLQKADLVIMMLDVSQGLTEED
                     LKVYELIKEQPTIIVINKTDLEQSIDLEEMKKEFKEHPLLWISVKEEEGIEELKNTIL
                     EEILHEQINVDSDVMVTKVRHRDALNKALDSIRKVKQSLDEGMPHDFLTIDLKDVLFY
                     LGTITGETVTDDIIDQIFSDFCLGK"
BASE COUNT        13258 a         9048 c         6641 g        14971 t
ORIGIN      
        1 gaattcgctt cccaactaaa tatatccttg gaagaattag atcgtgttta tgatgatttg
       61 gataacaatg gagacgatga tgaacaagaa gatatagatg atgatataga tgataatctt
      121 gatgatgacg aagacgatga tgatcaggat taagaaaatt tttctctgtt ataattatcc
      181 cttattttta aaaataaggg atttttattt gcattaaaaa atctcctgca gcatatcatg
      241 ccacaggaga tctatttaca acaaatttta aattgtcttt actctaataa atgacaggcc
      301 gcatagtggc cttcttcata ttctctgaat tctggttcct ctttcgcaca cctatcataa
      361 gcatgagggc atctggttct aaagctgcaa ccagaaggag gattaaccgg acttggaata
      421 tcacctttca gtgaaacttc acccatcggt acttttggat tcggttcagg cactgaatgt
      481 aataatcctt ttgtataagg atgaagcggt tcttcatata atttatcact attggtcatt
      541 tcaactatct tacctaagta cattaccgct accctatcac tgatatgttc tacaacagat
      601 aggtcatgag ctataaagat aaaggataga tcgaattctt cctgtaagtc cattaataga
      661 ttaataacct gagcctgaat agaaacgtct agagcagaga cgggctcatc acagataatc
      721 atcttgggat ctaaagctaa tgctcttgct acaccgatcc gctgtctctg tccacctgaa
      781 aactcatgag gatatctaaa catatattca ggtcttaaac ctacgataga catcagttct
      841 tctacccgtt ccgtgcggct ttgtttatta ccaacaccat gaattctcat accttcagat
      901 ataatattct taactgtcat acgagggttc agggaagcaa atgggtcctg aaagatcatc
      961 tgcatatctt ttcttagctt ctgtagttct gtctcttcta catgtgcaac atcctgcccc
     1021 ctaaaaaata ctcttccacc agtgggttca taaactctca ggatggttct gccggtagta
     1081 gatttaccac agccggattc acccactaat cccagagttt catttgcctt tagctcgaag
     1141 ctaatatcat caacagcttt taaatattca actttctgtt tgaaaaagcc tgaacgctgg
     1201 gggaagtatt tttttagatt ctctactttt aagattgttt ctttttcact cattacttac
     1261 tctccccctc atcaacatgt aaccagcatt tggcggtatg cctatctccc acatcgacct
     1321 caggcggttc tttttcctta cagatatcca ttgcatgttc acatcttgga ttaaatctgc
     1381 agccttgggg aaattcaaaa ggagaaggta ccataccttg aatagtatca agtctattaa
     1441 ttttcttgcc tatcttgggt attgatttga ttaacccttt tgtataagga tgttttggat
     1501 tttcatataa atcatctatt tgagcccttt caacaatctg tcctgcataa tatactaaaa
     1561 cacgttctac catctgagca attacaccca ggtcatgggt gataagcatg atagatgaat
     1621 taatttcctg tttcaattct ttcataagtt taagtatttg ggcctgaatc gtaacatcta
     1681 atgcagttgt aggttcatcg gcaatcagta aggcaggctg acaggctaaa gccattgcaa
     1741 tcataaccct ctgcctcata ccaccactga gctgataagg atatttctct atgacgctct
     1801 cttgatcagg aatacctacc agatccagaa tatcaagtgc tttattataa gcttcttggc
     1861 tgtttacatc ctggtgagtg gtataaactt caaccaactg ctttttaact ttaaataccg
     1921 gatttaatga agtcatcggt tcctgaaaga tcatcgaaat cttattaccc cggtaacttc
     1981 tcattttttc ttctttaata tctaaaaggt tttctccctg gaaaatgatc tctccatttt
     2041 caacataacc aacatcctgg ggaagcagcc tcatgatgga aagagaggta acactctttc
     2101 cacaacctga ttcacccaga attcccagag attcaccttt gtataaatca aagctaatat
     2161 cttctatagc tgtaacttta ccatcatctg tatcgaaact agtagttaca tttttcatct
     2221 ccaataattt ttctttttga ttactctgac tactcataaa cccacctcaa ttttctatat
     2281 atcattagtt atcccttact tcttctgcct tgggtcaagg ctgtctctta aaccatcacc
     2341 taagaggtta aatcctaata cactgaagat aatagcaata cccgggaatg taataatcca
     2401 gggtgcagtc agcattgcag aacgtgcatc agaaagcata actccccatt cgggtgtggg
     2461 tgcctgagca ccaagaccta aaaatcctaa ggccgccgca tttaaaatag cagtagcaaa
     2521 actcattgtg gtctgaacaa ttaaaggaga taaagagtta acaaagatat gtctggatat
     2581 taaccagaaa ttattggctc ccatcgtctt agcggccatt acatattctt cctctttgac
     2641 ggagagcacc tgtcctctga caactcttgc aaacctgggg ataccaacaa taccgatcgc
     2701 tatcatcgcg tttggtagac cccgacctaa aattgctact ataaagatcg ccaatagaat
     2761 atatggaaag gataataata tatccatgaa tctcataatt agggtatcta ttttaccacc
     2821 ataaaaccct gatactaagc ctaaaatagt accaacaaat aatgatatac caactgaaac
     2881 aaaaccaacc attaatgaca atctagctcc gtaaacaatc ctagaaaata ggtctctacc
     2941 catctgatct gtacctaaaa gatagaaatt atcggagcgc tgacggtcac tagcagtctt
     3001 ttcagtttga ccctggctag tttcttgttt gaatgcttca tcactggtac tgctttttga
     3061 ggaagaagtc cagccgccgg ccgaactatc agaactttca ctttcctcag tcgcaggttc
     3121 actaccggaa gaccaacccg aatcagagcc cgactgactt tgcgaggatt cctcttcagc
     3181 ttcaccggaa gtccaacttc cctgatcagc tgtttcctgc tgaccggttt gagtcgtgct
     3241 ctctttttca ctaacagccg gttgggcctg ttcaccttct tctgatcccc accaggtagg
     3301 tggtacacct tcatctccaa agtaaatctt agtaggatta aaaggagcaa ttatgggagc
     3361 aaagattgct accaaaacta aaacagccag cattatcatc cctaggactg ccagacgatt
     3421 tctcatcaag cgtttccagg catctttgac caaactagat ttcttagctt tttttacaac
     3481 ttctttttgg ttttcattct ttttcataat ttacaataac tcctcaattt atcaatattt
     3541 aatacgcgga tccaataaag aataagttaa atccgttatt agattaacca gtacaaatag
     3601 gaaagtaacg gttaaagttg ctccaattac aacgggaaaa tccctctgta aaattccgtc
     3661 taataaccat ttacccatac cgggccaggc aaagacggtt tcagttaata ccgctccggc
     3721 cattagtcta ccaaactgca taccaaaaac agtggtaatg gggatggcag cattcttgat
     3781 agcatggata aatacatctc taaatcttaa acctttagcc cgggcggtcc tcatgtaatc
     3841 ctgtcttaac acatctaaaa gagaagacct tgtcatacgg gcgataatag ccatgggaat
     3901 tgttcccagt gctataccgg gtaatataat atgtttaaca gcagaaataa atatgttcca
     3961 tttacctgta aataaacttt ctaataaata aaaatgcgaa cttaaaactg aaggatcaat
     4021 ctcagccgcc aatctaccgg ccgtgggtaa gatattaaga aaaactgcaa agaataaaat
     4081 aagcataaat cctaaccagt aaatcggcat tgatataccg aacaatgcaa tggtaatcga
     4141 gatataatcg aagattgaat tctgtttcca ggctgaaatt actccggcca aacctcctac
     4201 tattaaagca tatatcatgg caaaagttga tagttcaatc gtagccggta atttttcttt
     4261 taagaccgtg gaaacaggtc ttttggaaat tagggattct ccaaaatcca atctgacaat
     4321 ctcagttaaa aatctaccgt atctagttaa aaagggatca tttaatccca tcctctcccg
     4381 ggccgcttcc agtgcttctt gtgaaacatg ctgtccaagc ataatttggg ccggatcacc
     4441 gggaattaaa ttaaccatac caaatattaa aaaagacatt attaataata ctggtattat
     4501 ataaatcaat cgttttatta tataccttaa catgtaagaa ctctcctaat tctaaattat
     4561 gttgtattaa ttaaaaaata tagtaaacct gctgattgaa aagaacatac aaccagcagg
     4621 taatctttca aatgttgaaa ataaccttac ttttaattat tgttcaatcc atgtattttg
     4681 taaataaact ccaccggttg ggtgaattac aaatccgttt acatcttgag ctgtacccat
     4741 cgctttcata acgtgactta atggtaccca gggagcatct tcatgaatta catgttgagc
     4801 tgccttatac ataccagctc ttaatctttg atctcccatg gtctgctggg cttgaattaa
     4861 gatattgtgg agttcatcac ttctatagaa ggcaatatta cctgcactac cttttttggt
     4921 attatcttta tcaagtagaa cgtaaatgaa gttatcaggg tctccattgt caccgatcca
     4981 acctaataat gccatatcat gctcagcatt ttcagttttc tctaaataag taccccaatc
     5041 atatgtgact agctcaatgt caacaccaat gtctgcaaga ttactttgta ctgcctgagc
     5101 tactttctta ccgttaggca tatatggtct tggattactc atataccata attcagcaga
     5161 aaatccatct ggatagcctg ctttttctag taactcttta gctttttcag gattataacc
     5221 atagtcttct acttcgtcgt tataacccca gagagtaggt ggtagtgggt tcttagcagg
     5281 ttctgcagta cctttataag caccctctat tattgcttct ttatctacgg ctaagtttat
     5341 agcctgtctt actaatttat tgtcgaaagg ttcctttaaa ttattcattg caagataacc
     5401 aacattcata ccgggttggg ttaagagctt caagttatca tcagatctaa ctgattcgat
     5461 attttgtggg ccaagaccgt caatcatatc aaggtctcca cctttaagac ccattactct
     5521 tgtagtattc tcaggtacta cactgaatac taccttatct aagtatgcat tttctcccca
     5581 gtaattttca aacctttcta atgtaatagt tgaaccatgt tcccagttga cgaattcaaa
     5641 tggtccggtt ccaactggac ttgtcctaaa tccttctaca cccttttcct tcatagcttc
     5701 ttctgaaact ataaaagcag ggaatgtagc caagttatag atgaatggag catatggtct
     5761 agaaagggta atcttaactt ggtaatcatt gatcttttcg accgactcta ccatattgta
     5821 catgtaaccc caataagcga agtcaccttt atggtatggg tggtcttcat tcatctggcg
     5881 tctgaaggtg aatactacat cgtcagctgt aaattctgaa ccatcgtgga atgtaacacc
     5941 ctctcggagt ttaaattccc aaactttgcc ttcttcgcta acacgccatt gtgtagcgag
     6001 ggcaggttta acttcagtac taccttcctt gtacttaact agaccttcaa aaacatttgt
     6061 aataacttta actgattcac catcagtcac atcggcagga tctaaacgta ctgcatctga
     6121 ccctctaccg aagacaagag ttccgccttt ttgcggctcc tgtgctaatg cggtcccaaa
     6181 taaaccaaca actaatgctg ctactaatcc taatactaat agagttcttc tgctcaactt
     6241 ataatctcct cctctatact atttatgcta attcttatat ataaaaatgg taacaactgc
     6301 tactgtatat gtcaagtgta tgagctcttt aaaaattttt agaaaattta ttaaaacaaa
     6361 aatagccgat tcaaccccat cctattacta aagatgaagt atctatcggc tattttctta
     6421 atagttaaaa ttataatttt ttattgtatt actagtctaa aatactattt agaaaccagg
     6481 tcacaatgct caataaaatt gctccaaaaa ctgcgctcca gaaaccggcc accataaaac
     6541 cgggtactaa tgatgctgaa agcatgagca tgaggccgtt aacaaccagc agaaataatc
     6601 ccattgttaa aatagttaag ggtaaggtta aaacaatgaa aattggttta actactacat
     6661 ttacaatacc caagatcaag gctgctacaa agccggcaaa aagtccttga atggacattc
     6721 cgggtataat atatgctgtg ataaagagag ctaccatcgt aattaataat ttcgccagta
     6781 aacctttcct tttcttttta aattcatatt ggtaagtggc catgaaatcc cccctataga
     6841 tatttgtcta aacctttgtc ttttaagagc tttctcagct tttttacatc ctgagcatta
     6901 tctttattac agactaaaac agcatcttcc gtatttacta tgattaaatt atctacacct
     6961 atggaagtaa ctaatttatc ttcactatag ataatggtat cttctgtatc aatactcaca
     7021 tgctcggagt tggcatttac aatattattg tcctgatcct ttttgcctac ttctcctaag
     7081 gctgtccagc ttcctacatc attccagcca aaatcagccg ggattacaaa gatttcattc
     7141 tctttttcta agacaccata gtcaattgaa attcctgtta tacatctgaa ctgttcatca
     7201 ataacatctt cttcattgtc tgtcccaatt gcttttgcta tttttgaaag tgtttcatat
     7261 atttcaggta gatgctcttt gatcttattt aaaatggtct tggtcttcca gacgaacatg
     7321 ccgctattcc agtaataatt accgctttca aaaaaatcgt ttgccttttc ataatcaggc
     7381 ttttctgtga attccttgac cttaaatatc ttattaccct taatgttatg gacctcttca
     7441 ccccggtgga tatatccata gcctgtttca gggcggttcg gctttatacc cagggtaaca
     7501 atattttctc cctgcttcgc ataatcagct gcgctatcta atatatctat aaagttatca
     7561 ttgtctttta taacatggtc agagggtagt actaccatcg tagattccgg atatttacgg
     7621 ttaatataaa tagacgccag gccaatacaa ggggccgtat ttcttttaca gggttctact
     7681 ataatatttt cacgcggtaa atcaggtaac tgcttggcga tcttatcaac atagcggtca
     7741 ttggtagcaa taaagaccct atcaatatct accatctctc ttacccgatc tactgtgcac
     7801 tgcagcattg ttttttcatc actggttaat tttaaaaact gcttgggatt gtccatacga
     7861 cttaccggcc aaaagcgggt ccccactccc ccggccataa ttagtactgt cttcattctt
     7921 acaacactcc atttatatta ttttcttgtc taatactaat attttaaatc aagaaatcta
     7981 ctatcattac ctcaataata tattacaata gttgcaaaca gattccttct tttaatcata
     8041 ataaacaatt taacagccat tttcaatcca ttttgccatt tgtttttagt ttttaaattt
     8101 tacctggctt aaaataatat ttaaggtaaa tctttaattt aaagtaaaaa gaaggaatta
     8161 ttatattttt gtacaattat tatctataac ctaagaaagg agaagtttga gatgaaaaaa
     8221 ttactgatac tcctttttgc ctttatctta ttatttatac ctgttagaac gaatgcttat
     8281 gaggttgact ttaatctgga aagcaagtta ggcgacttgg tggttactaa tactcccccg
     8341 gttggtaata tagaagagaa caacctctct ttaaatacta tcatgttgaa agcaaatttc
     8401 aaccagtata tttttagaga atgggctgat ttttattcta tttccctggg tacaaactta
     8461 ttagatacca gtactgaaga tactgaaata atctattatg aggaaagttt atataataag
     8521 gtagataaat taaagggaaa tacagaatta aacagtttct attttgacct gttattggct
     8581 aattaccttt tagattactc gacaccaagg gaaagttatt taatcctgat cggatatgaa
     8641 tatgataact ttaattatga gactaaagaa aatattaaaa ttgctagtca tgatatccag
     8701 actcatatcc cttatatagg ttttatctat aatagaaact ttacaagaaa tataaaaaac
     8761 agatttatct tcaaattttc tccctatacc tatacaaata attcgagtaa aaactataat
     8821 aacacttata taaatgatag taccagtgat ggtaattact tctcacttaa aaataatgtg
     8881 cggtacaaaa taaataaaga tatgtatttt acgggtggtg gccagtttaa agtattaaaa
     8941 accactggtt ctggcaccag atattattat agtggggtta atgaaggtat ctcctataat
     9001 ataaactctg aaacagaagt tgaagagtat agtttaaaca ttggaattca atatcttttc
     9061 taaaatatta aaatgaagct cccttacagg gggctttttt ttatgcctaa actttttaat
     9121 tatcattaac tagtcagtaa aaacaatact ttttcaacta ttttaactga gtcctgaata
     9181 actaatctct ataatccata taaaagataa actatatcct attttattaa aaatgttaaa
     9241 taaatgttaa cacacttgac atttagtctt aattccttat aatagttaga ggaaataatt
     9301 gaatacaatt gaaaggaggt catctagatg aacttcggta aaaagataaa atgtaaagta
     9361 tgtggaacaa gaaattggtc cggtaatgag tactgcgaaa agtgcagtgc gaaactagac
     9421 tataacaata ttgagaacat ccaggaaaaa gtaagaaaga agattgctta acttgcctat
     9481 tagttagcta tccttttatc tacccttatt ttctcctctc tcttttccct tcttctttcc
     9541 tacctacata atctttaata ttccaatcct aatcaaacat tgacaagact cgggtattaa
     9601 tttacaatta ttacagtaga taatattaaa aattgaggtt gggatattta tggataatat
     9661 ttacttagat aacgctgcca ccaccaaagt cataccggaa gtattagaaa aaatctgcga
     9721 agtattagaa actaactttg caaacccttc ttctctacat ggattaggta ttgattctga
     9781 gaaaaaaatt aaaaatgcga gaagcaagat agctaatgct ttaaatataa aaccagctga
     9841 attaattttt acggggagtg gaactattgc caataatatt gctattcaag gtacagttaa
     9901 tagattaaag aaatttggca aaaaaattat tacttccaaa atagaacaca aatccgtcct
     9961 ggaaatattt aagcattttg aaaaggaagg ttttgaggta atctatctcc cggttgacca
    10021 aaatggaatg attgatcttg ataaattaaa agagaccatt gataaagaaa ctatcctggt
    10081 cagtataatg gcagttaata acgaattagg gtcataccaa cccttaaaag agatctcaaa
    10141 tattatcaat aaatatgata acctctattt tcatgttgac ggtgtccagg ccctgggaaa
    10201 agtttctctt gaccttaagg gcttaaatat agatcttttc actgtcagca gccataaagt
    10261 tcacgggcca aaaggggtag gggctctcta tctcaataat gacattatca ttgataagat
    10321 actttttggc agtagtcagg aaaaaggttt ctacccgggc acggaaaata cggcgggtat
    10381 agcaggcttt ggcaaagcag tagaacacct accgactcaa ccagaaatta aacagataaa
    10441 atctttacgt aatatgttga tcgataaaat aaaagcagag atccctgata tcataataaa
    10501 tacaccttca ggagaaaacg ctgcccctca tatactaaat gtttctttta aaaatattaa
    10561 aggagaagtt ctggtacata gtctagaaaa agatggaatc tttgtctcaa ccggttctgc
    10621 ctgcacctcc cgcaaggatt cgatcagcca cgttattcaa gccttaaatc tcccggaagg
    10681 atatcgggat ggttcaatta gatttagttt tagccaatat aataccaagc aggaagtaga
    10741 atatactttt aaatcaataa aagaaaatgt agccaactta agaaagatca tggggtgaaa
    10801 aaatgagtaa tttatattta atacgttatg gagagatagg tttaaaagga gccaaccgaa
    10861 atatatttga agataaatta atgtccaata taaaaaaagc agttgaatgg gaagatgaag
    10921 accttaatat ttacaaaaca cccggccgta ttttcttaga aacttctgct gacaaaaaga
    10981 tcgtcaaaga aagactttta aaaatcgccg gtttggtctc tttttcgcct gtgaaaaaac
    11041 tgcctttgga ttatgcacaa atcaaaaaag aagggctggc tgcagcagaa aaatttattg
    11101 ctgataagga tagaataact tttagaatta gtgcgcgaag aagtaataag gaatttgaat
    11161 atgattccat ggagcttcaa tatgatttgg gggaatatgt tttagaaaat attggggaag
    11221 ataagatcga agtggactta cacgaaccgg atttagatat aaatgttgaa attaggaaaa
    11281 aacacgctta tgtttattca gaaacctttg aaggtatggg cggactcccg gttggcacca
    11341 ccggtaatgt tgggctgatg ctttccggcg gtatagacag ccctgtagcg ggctatatgg
    11401 gccttaaaag aggagtaaga ctaatacccg tctattttca cagctttccc tttacaagtg
    11461 atcgggccaa agaaaaagta attgatctag cagaagtatt aggccaatat caaaatgata
    11521 tagatttaca tgtggttaac tttacagaaa ttttaaaaga aataaatgag aagtgtccag
    11581 aaaaattaat cacaatcata atgaggagat tgatgattaa aattaccgaa aaaataatag
    11641 agaagcaaaa tggaaaggcg ataataaccg gagaaagcat aggtcaggtg gccagtcaaa
    11701 ctttagaagc cataaaagtt accaatcatg ggccggatct gccgatacta agacctttat
    11761 tgggacttaa caaggtccag attatcaaac tggctaaaga aattggcacc tatgatattt
    11821 cgattcaacc ctatgaagac tgttgtactg tttttgtgcc agatagtccc gaaaccagac
    11881 caaccctgca acaaataaaa gaaggggaag ccgaccttca tgtcgaggac ctcattgaag
    11941 aggctgtggc aaatgtggaa gtaattaata tccaacctta ttcttaagga ttatttctca
    12001 ttaccgctga aaacatttat tttattgaaa atatcccaaa tagattttga tttaaaatgg
    12061 gtaggctctt catagataat ttcttcatca ttaattatat attcagcatt agtaaagaac
    12121 tggacggtat agcttccaca tatatcttcc agttttcttt ttgccctcgc caaccattcc
    12181 aggttaactt tcgaactgcc gtgacagtct gaggcaataa cctgtactaa ataattatct
    12241 accagttcaa aagcggtctt ttttacatta ttaccataaa ctccaaatag actgccggca
    12301 tttaactgaa aaatgatgcc gtctttaacc cattcatata aaatatcagg atttttaatc
    12361 acttcctcat ttctttccgg atgggcaata accggcttat aacctaaaac actcaaatca
    12421 taaaatactt gatctaaata ttcggggata taattcatag gcatctccaa tagtagatat
    12481 ttgctgttat tgatgcttaa aatatctttg tttttaaact tctttatcaa atcgggagtt
    12541 atataaattt catgtcctgc taaaatttcc acattaatgt cttttttatt taacagttca
    12601 tttaattctc ttactttctt ttttatggtc tcaccctcaa tctgataact ttcttccata
    12661 taatgagggg ttgccactat tttatggatt tcatattcct ctgcggtctt aataatttct
    12721 aaagtttctg actgggattt agcaccatca tcaacctcag gcagaaggtg attatgaata
    12781 tcaatcataa aactcaccct tttttagtaa tttatggatt ctctcctgga aactccaata
    12841 tctgtcctat agtgcatatc ttcaaagtaa acttcgtcaa tatattcgta aattaggcta
    12901 atagcatcat ctaagttatc acctaataca gttaatccca agactctacc accggccgtg
    12961 tagtatctat cgccaatttt agttgtacca gcatgaaaga ccaataaatt atcataacgg
    13021 ttaatctgat ctaaaccttt aatctcttta ccggtatcaa aagtgagagg atatccggct
    13081 gaagttaaaa ttacacagac agctgctctg gtatcccatt ctatttcaac atcttccaac
    13141 tcttcatcaa ttgtcttatt aaagatgtca attatgtcat tgttcatcct tggtaagata
    13201 acttgtgact cgggatctcc aaaacgggca ttaaagtcta atacttccgg ccccatatcc
    13261 gttaatatca tgtttatata caagatacct ttatagttta aaccttcttc attaaatgct
    13321 tctaaagcag gttttaatat ttcattatga atacgctttt taagcttttc attgacaaaa
    13381 ggtaaaggtg aataagaacc cataccaccg gtatttggac cttcatcacc atcaaataca
    13441 gctttgtggt cctgggcaaa ctctaaggga acacagtttt tcccgtcggt aaagaccatt
    13501 acagaaattt cttcaccttc cagaaagttt tcaatgacaa tccggctgcc ggcatcacca
    13561 aaaaccttat cttccataat cctggtaatt gcttcttcag cctgagattt attggtggca
    13621 ataactactc cttttccaaa agcaagtcct tctgctttta ttacgagcgg ataatctgcc
    13681 tcttttaaaa attccatggc ctcggctggt tctgtaaata cttcaaattc agctgttgga
    13741 atattatgtt tttctaaaat atttttggca aataccttgc tgccctcaac tcttgcagca
    13801 agtttattgg gcccaaagat tcttaaacct cttttttcga actcattaac aatacctttg
    13861 accagtggtt tctctggccc tactacagtt aaatcaatct tatgctcata agcccaatcg
    13921 gcaagcgcag taatatcact ggcggcaata tcaactctct ctgccaggtc agtcatcccg
    13981 tcattacctg gggcaacata gattttatct actttttcac tttgatagat cttccatatc
    14041 aaagcatgtt cacgtccacc gtttccaaca actaatactt tcatggttta cactccttat
    14101 attaataatt ggagaatagt ctctcccttg tttagattaa aactaatctc attttcttat
    14161 aatagcatca tctcgttcag gtccggttcc aataactgtt atcggtattt caatctcctt
    14221 ttcaatcata tttatataat ttttagcttc tttaggtagt ttatcaaaat cagttaaacc
    14281 agatatatct ttttcccaac ccgcaaaact ttcatattgg ggagctcttt ctccctttaa
    14341 accagcctgg gaaatagtgc agtcaccttc tttataggaa gtacacactt taatttgctc
    14401 taaaccgctt aaaacatcta ctttagttaa tactaattca gttaaaccgt taactctaac
    14461 cgcatacttt aacagcggta agtctaacca gccgcatctt ctgggtcggc cggttgttac
    14521 cccatattca tggccttttt ccagtaaaaa tgttccgttc tcattaaaca gttcggtagg
    14581 gaatggtcct gcccctaccc gggttaaata tgcctttgct acccctatta tatcatcaat
    14641 tttggtagga ccaaaaccag ctccggtaca aactccaccg gaagtaggat ttgaagaagt
    14701 aacatagggg taagtaccat aatctatatc taagagggta ccctgggctc cctcgaaaaa
    14761 gatattctta tcttttttat aggcttcatc taaaagcaag gatgtatcgg ttacataagg
    14821 cttgatcttt ttagcagctt caagatactg ttctaagatc tcctcataat taaattttgg
    14881 tttgtcatat actttactaa ttatcaaatt cttgtattct aagatgtttt taagtttttc
    14941 cttaaaaact tcttcatcag ttaagtcaca gattctaatg ccccggcggg cggccttgtc
    15001 ggtataggta ggccctatcc cttttttggt tgtccctatc ttattatctc ctttgcgttc
    15061 ttcttctaag ccgtcgaatg ttttatgata gggcataact acatgggcct gttcactgat
    15121 atgaaaatta tcaaaggtaa tacctctttc ctttaatcca gctatttctt cattaagagc
    15181 aactggatct attacaacac cattaccgat cacacttttt ttctcaggat aaagaattcc
    15241 agaaggaatt aaatgtagtt catatttttc atctcctact attacggtgt ggcctgcatt
    15301 attacctccg ctgaatctta ctacatagtc ggctgtttca gctagtttat ctgttacttt
    15361 tccttttcct tcatctcccc attgtgcacc tattactact ttaactgcca tatctatgct
    15421 taagtatatt tacttaagct tccccctttc aaatatgtaa tcaacttctt ttatgaaatt
    15481 ctcataatca aatattttat ttaactcttc tttatttaag tatgtcataa cttccccatc
    15541 attttcaatc tttttttgga aactttcatt attttcccaa gcttttaggg catttctctg
    15601 aactaactca taagccctct ctctttttaa acctttatcg attaaagcca gcatgacctt
    15661 ctgcgaataa attaagccat tggttttatt catattgatt tgcatggttt cttcattaac
    15721 tttcagatta tctaacacat ctttgaactt attaatcata taatctaata ggatgaaact
    15781 gtcgggcagg ataattcttt ccactgaaga atgggttaaa tctctttcat gccagaggtt
    15841 gacattttca aaggccggga ttaaattgcc cctaattact ctagccagac cgctgatcct
    15901 ttcagtcacg atcggatttt tcttgtgggg catagcagaa gaaccctttt gaccctttga
    15961 gaaattctct tcaacttcta aaatatcggt cctctgtaga tttctaatct cggtggcaaa
    16021 cttttctaaa gaagcagccg ccaaactcaa cttactcatt agatcggcgt gccggtctct
    16081 ctgtaggatc tgattggtaa tcatagccgt atctaaattt aactcttgca tgaccagctt
    16141 ttctacttcg ggatttattg tggcataggt gccaaccgca cccgaaatct gtcccacttt
    16201 aataacttct gccagctgat tgaatctttt ctcatgacgt tctatctctt taaaccaaac
    16261 cgctaatttt aagccgaaag taatcggttc agcgtgaatt ccatgggttc tcccgatcat
    16321 taccgttttt ttatgtaata atgctttttc ttttatcgat ctttttaatt ttcttagatc
    16381 ttttaaaatt aaatctaagg cttcacttaa ctgtaatgct aagccggtat cctttatatc
    16441 attggaagta atcccttcat gaatatactt tgcttcattt cccaagttct cattaaccgt
    16501 ttcgataaag gctaacatgt catgtcgagt ctctttttca atttctttta tccttgcaac
    16561 ttcaaagctg gcattgtctg ctattttttg gtaggcttct tcaggtactt cccccaattt
    16621 ttggcgggct tttaccacag ccagttcgac atccagccac ttttggaact tgttttcttc
    16681 actaaatata tttttcatct ccgcgcgcga gtatctatca agcaagctaa tcttcctttc
    16741 tgtatttatc ttatccgact taatattagc aaatatggtt atgggtgtca agttagtaaa
    16801 tatgcatcaa tgaatagcag ttaaccaaaa ttttgcattg tattttcagc ccaaaagatg
    16861 tataattttt tacgagggtg agtaaaaatt ggaaaatatt aaattaatcg accacaaaaa
    16921 aattagaata tgctgtgacc atgaacaaca gagtatagag ttgggaagag aaagtataaa
    16981 tcatggtttt ttaaaaaccc tgccgggccc ttcactaccg gtctttttat atcttataac
    17041 ccatcttgat aataataatt ctataaaaac taatcctgca cttatggcag gatttttagc
    17101 cctggaccgt gaacagataa aagaaggctt aaagatattg gaaaataaag actatataac
    17161 tttaaaagaa aaaaaagaag gtaactatac atataaaata aagttgaatt tagataaaat
    17221 tgttggttcc tgttactgcg aaagtgatga aagtcaagat gaagacttaa aagaatacag
    17281 acaagaagta ataaatcata ataacttaaa agaagaagca tataaaaaag ccctgatcag
    17341 ttttttacca aaaggagtgg aacattcttt aatagaagaa gaaatcgaac actggttaaa
    17401 tgattttgat aaagatatga taagagaact catcaggcgg gtcgataaat ggataaataa
    17461 taataataaa agtagtaaag aaggctttta ttatctccac gggatcatcg atgactggta
    17521 caataaagag atctttgatt atgataggtt aaagtatttt gatagaatgt acagggaaac
    17581 caatgaactg gccaaatgtt acggccttaa aaactggtac aatgtgagcg aatcacaaat
    17641 ggaaatcttt tatgattggc ttaatgataa taatggaatt aatctttccg ttgccaagtt
    17701 tgccatcaaa gaggccttta aaagaaaaag tgatggacaa ccttccttaa aatatattga
    17761 agataacttt atcagcccct taaaggaaaa caatataaga aatattaatc aggcaaaaaa
    17821 agttttaaat aataattcat ttcaagctga acaaaccgat caaacaaata aagggtcaga
    17881 aaccagctgg gaaacctttg agtgggatat tgaagacctt aattaacgtt atacaggagg
    17941 aaaaaagatg gctttagaac aaaattatcc cgaacaatta gaagataatg ttgaacagga
    18001 atttcaactg ctctcaatat tacttcagta ccctgataaa attgatgaag ttgccgatat
    18061 tttagaaacc agacactttt taaatccaaa agcccaacta atctatgata tattattaaa
    18121 acaatttgaa cgggacaacc aaatttcaaa aaccaaatta tttttaaaac ttaaaaatga
    18181 aaatatcgtg aaaagaccgg aagaaatgat cgaaaattta acttcaggtt tcaatacccc
    18241 ggaagaactg aaacccacta tcgatattat aaagaaaaac catcaaaaag accttttaat
    18301 ccgggcctct aaaaaaatag ataaattagc aacacagact gaattagata tctcagaata
    18361 tcagtcaagg gcccaggaaa tcatctttac ggccactaat gaatcttctg accttgataa
    18421 acatatctat acaatggagg aagccctact agagtccttt gaagcttatc taaatagaaa
    18481 agaaaaaaag gttgatgtgg gattacagac cggctttatg tccctcgata atttaatcgg
    18541 tggttttaaa aaaggtcatc taaatgtgat cgctgcttct accagtatgg gtaaaactgc
    18601 ttttgcaatt aatatagcta aaaatgtcct gaagagaaat gaaccaactg ctatcataag
    18661 tctggaaatg gaagcaccgg aaattgtaga taggatgatt attcaggaag ctcaagttaa
    18721 cggctggaag tataatcagg gtgaaacaac cgatgaagaa gataaaagaa tttcaaaagc
    18781 tctagatgat ttacatgaat tcccactggc tatctcagat gaaaggggtc taaatgtagc
    18841 tcaaattaga gcccgtttaa gaaagtttaa agcccaattg gatgaattaa gtctggttat
    18901 tatcgattac ctccagatga ttcagctccc ggaagaacac gcccaaaata cagcccgcgc
    18961 tgttggtgaa atagtgctgc agttaagaaa tttagcatct gaattagaag ttcccatcgt
    19021 acttatctca cagatcagta gaagctttac ttcaagacag gataaaagac cggtcctctc
    19081 tgacttaaga gactccggta atattgaaga agtggccgat ggagtaattt tcttatacag
    19141 acatgcccac accagtgcag ccgcccggga aaaagcagaa gcggaaggta cagaacagga
    19201 tacagaaatt attatagcaa aacagcgtac cggtcaaacc ggttctgtta aactgctatt
    19261 tgaagaggat tacatctctt ttgttgatcc cgaaaatgta tcttttgaag gcatgatacc
    19321 aaatgagtaa agatctcgac ttaaaaatta tctatgaatt tacagataaa tatgaacaat
    19381 taaaaaaaga agggaaaatc tcggaaaaac agtttaacga gattctttcc cttcttgata
    19441 atttagagaa gttctctaaa gaagaacttg acgataaatt agaagacata acttagttct
    19501 atatttaaag ccgctttggg caaacctctg cccgctccaa aatcgctggt ttcagaacca
    19561 cttaaataag aaacatttat atttaaggta gtattagtac tgagttggtt actataattt
    19621 ggaatcaagg cagtactgcc gtcttctaaa gaagttaatg taaaaaagcc gatcgagctg
    19681 aagtcattga tactataatt tacattggtt aaaaatatat cgagatattc atctccacct
    19741 aaaaatttcc tgtaatctaa accaattcca gctcctgcta gagattcaat taagactgca
    19801 agcccttcat ttttttctac taaatgatac tctccctgca tataaagtct attaccaaaa
    19861 ccctggttta aaaagtagct gtagtcagtc ccaaccataa aagcctgtgt attaacatcg
    19921 gagaccatag gagttttata gtttatattt gtataggaac catatacacc agcatttttt
    19981 atatcacctt tcagtgaaag cccccaccta tttatatcac tttcaactgc atcttgagtt
    20041 acataattaa aactcacatc ataatcctta attaaagtcc tagccctaaa tccatattta
    20101 tcataactat tattaaattc agtcacaaaa gaaacattgc tgtaccagtt gatgggataa
    20161 tgaaattcaa ctgcatcaac atacttggca gaagtttctt catcgagggc ttctgcgcct
    20221 agactgtaat caattggatt aatcaaagaa ccgaatgacc aggagatagg ttgtctaccc
    20281 agggttaccc tcgttccttt aaatttatga cgtacataag ctttttttaa gttgatttta
    20341 aattcttgtt ttccattatt attgctacct gtaatctcaa agttggcatt attaaagtct
    20401 aaattgagat taattgtttc ataaatactg ttaatgttcg tctcatcaaa aaatactgaa
    20461 tttaaaccag ttgagacttc tcctccgaac tgataagaag aggccagagc tgtcgtggtg
    20521 gtgaaaagta aagtgattgt taatatgaag ataagtgatt ttttagttaa caatttctaa
    20581 acctcccctt tcattattgt aaggattcta atgagaatgt tccttctgga atttcgggat
    20641 tgaattcgat agaatctata ataaaggtag tactggtgtt ttctctcaat ttattttcca
    20701 tgatagttct aattggatac catctaccct caatttcttc tacttcagtt acttctgcta
    20761 ctttaagtaa cttgccgctt tctgcataaa gttcttctct taatccaata aatctttctt
    20821 tatctatcca accaattctc ttatagtagg atacttctgc cccttcttcc ttgattgctt
    20881 caatcttata acagggtctg ccattatatt ctacttctcc taccagttca aaattataca
    20941 gttctgtaag tttttcggat tccatcatat ccttatagga gaaatcacta cccatcatcc
    21001 ctctctccaa catgtgtccg gatagtttta ccacatcctc agcatctggg aaaaacatcc
    21061 agagattatc ttctcttttt aaaaattttg tgcctctatc catcgggtta gtgaatctaa
    21121 cgagggcttt atctccctca attaaggaaa ccatcttctt ttctaattcg cggccagcgt
    21181 tattaatgat catagttgat tctacccgcg cctgttttag atattcgtta tcatcccttt
    21241 tgttaataat ttcttcagcc gttatagcca gagctgaaga actgaaaata aatagggata
    21301 aaataattaa agctgtcact tttttaaatt tcataatatt acacctccat taaatatctc
    21361 ttaaagcatc attaggttct agattcgccg ctcttctggc gggaattata caggttaaag
    21421 ctgtcacaat tgtacctagg ataaatccaa agattacatt ttctaaatta tagaccgggt
    21481 aaatcatcgg attcataaat acttcatcga taacaccttc aacagcctgt gtataatcaa
    21541 ttccatgtac agccagatat tttgttaatg ctccacctga tacagaacct attaaactac
    21601 caacaagtcc cataaatgta ccttcaatca taaacatagt taaaatttct ctgcttttca
    21661 agccaagagc ttgcatcata ccaatttcct gggtccgctc ttttacaatc atgaccattg
    21721 tgtttatcac aacaattgca gctaataata ataataatat atagatgaca ttgtagatat
    21781 atttagcaac atcaaataat tttaaaagag gattggcttc tccccaggtc tgaactgcgt
    21841 atttgcctct cccatcattg gattgaatta attgttctat ctggggttct attgactggg
    21901 ccgctgacat cgatttggtt tttaaaagaa tttcagttgc catatcctgc attagaagaa
    21961 ttctctgggc ctgatctatt ggtaaataaa ataatctatc attgattagg ggcaagtaat
    22021 ggtctacttt acctactatt ttaaatgtgg agcctttaaa ggcatcaaag gaatttttat
    22081 ataatattgt aaccttgtct cccacatttt tatttaaaga atccatgagt cctgtgccca
    22141 tcacaacttc tctttgcccc atttctatca ttctgccttc aactaatttg ctttctattt
    22201 cagtaaactc tatttctttc tctggtaata ccccccagcc taacattcgg acaatctctt
    22261 catcggggct tgccatcgca ccaaatctta atcgcgggat tgctaattcc acaccctctt
    22321 ctgcttccag cttctctacc ataggcatga tacccttgct gttaaatcca tcaacagggt
    22381 ggatcaaaga taagagcttt tctttttggt catacttttc atcgataatc ctaatatgac
    22441 ccgaatcata ctgaacatat aatgaaaagg aagagtttaa aactcccatt acgagacctc
    22501 tcgcaaatat aacaaccatt actgcaattg caatcgctgt aattgaaacg atggttctca
    22561 ttttattcct aaagagattt ctaccggcca gggttattaa aaatttcata tattataacc
    22621 tcccttatct gtggtatatt gccttgactg gatctttttt tgctccccat cttgccggta
    22681 agatactggc taaggtagaa attataatcc cgaagataaa aatgaataaa aaagcttccg
    22741 ggtgccagcc gccgtatatt ttaccggcta tcggcattcc aaaggaggta gttccccctg
    22801 tcattgcaga aatacttatt ccaatatttt tcaacagata aactccaatt gctccgaata
    22861 ttattccaat tgttgagcca ataactccaa taccagctga ttctataaca aagatatata
    22921 ttatttcacg agcctttaat cctaaggcct tcatcatacc gatttcttct aatctttcta
    22981 aagctgctaa gattaccgtg tttataaccc ctactgtggc cagtaaaatt ataattgcta
    23041 acataacaag gttttctata ttaccctgtc taaccatggc caggacttcc tttgataact
    23101 gctcccagtt ataggctttt atacttgatc cggcctgttt aaaatcttca tttaaatttg
    23161 ctgccatttc aacagattga tccctgtttt ctaatctaaa caaatacttg ctcaccttat
    23221 tttctaaatt caaggagtcc tgagctaaag atagaggtat atatacatac atttcattaa
    23281 tcatcggatt aggggtgtca agcacacctt gcagttcggc atcaatggta ttaaaagtac
    23341 cttcttcagt cttaaataat aaggttacat aatctcccac ttcaaaaccc atcatattag
    23401 ccaaactttt cccgatcatc gctttctgcc ccgaacttaa catttcaccc tttaaaatat
    23461 attcttctaa ttcaaatata tcaatttctt ttttaggttg aatcccaatt acttgaatcg
    23521 gatattcttc ctgcccatta ttaatcgtag cagaaaaatt tagacgggca gtatgttggg
    23581 caatattcga atatttatta tctatttgat taataacttc attattctca tttaaaagat
    23641 aatcaagagg ggcttcctct ttctcatccc aataatcttc ttccataatc tgcagatgag
    23701 cagtttcaag ttcgatatta ttgtccaaag atatatctct tagcccaatc attaaggaat
    23761 cagataataa gaaaataaag ataccgaagg atataactag ggcggtaatt gctgtccttt
    23821 ttttatgtct agctagattt ttgattgcta gtttagtgag gaacatccag atcctctccc
    23881 tttttatcag tagtcacttt accgtctctt atttcgatta atcttctagc gtacttcata
    23941 acctgaggat catgggtaga gaatataaag gtagttccta acttttgatt catcttgagc
    24001 attatgtcca atacttcttc actagtttca gaatctaagt tggcggtggg ttcatctgcc
    24061 aagacaagtt tgggattttt aactaaagcc cgggcaatag ctactctctg tttttggccg
    24121 ccggacagtt cattcggtct tctatcttct aaaccttcca acccgactga atccagaata
    24181 tccaatactt tttctctgcg ttctttttta gtcatctcat caataattct gatggcaaat
    24241 tcgacatttt caaaggctgt tagaaccggt attaaattat gaccttgaaa gataaaaccc
    24301 agattgtacc ttctgaaatc agataattca tttttggata gctccgataa tttttttgtg
    24361 ttattataat gaatttcacc agcagacggc ttatccaaac aaccaattat attaagtaat
    24421 gtggttttac cggagccaga aggtccaaaa atagtggtga attctccttt ttcaatttct
    24481 attgagatcc ctctgatagc aggaacatct attttcccct ggtggtaagt ctttttaaca
    24541 ttttcaagct taagtaatgg cattttttaa cctccctctt ttttaattcc gtattgaatt
    24601 atttcaatta aattgtcaat aagtggtata aattcatcta actgctttat atctttcttt
    24661 tcaaaaaagt aatcggtaat aaaaaaactt gccctatata aaagaccggc tgtataagtt
    24721 atattaatgt cttttctaac ctggccggct tccacagctt ttttcagtaa tctttcaaac
    24781 ataccttctc ctagattttt atattcatca attattgatc ggtaaaattt ttgatcttta
    24841 ttttttacaa tatcacttgc cacctctgct aatttgatat tatcttcagc atactttagt
    24901 ccggcaatat ttaatctacg ccaatactca aaaaaatcat cgccttcata atcatttaat
    24961 tccttttgta catatttgaa ctttttttca acagcctttt ttaaaatatg tctatataag
    25021 tcttctttat cttcaaaata ttgataaaaa ctgccttttg ctatctgcgc tttattcacc
    25081 attctagtga tacttgactt tgtatatcca ttttgtgcaa actcatcgac agcagcatta
    25141 atgacccttt ttttcttttc ttccggtaga ttaaaaaaag ttgtggtcgg caattacttt
    25201 acctccctta tttatgactt tatattcatg tgactttcta ttcatattat aaacctataa
    25261 ggctgacatg tcaattaaaa tgattaaaag aattacattt atttaagtaa aattgaatta
    25321 ttgagaaaat aattcacttg cctattagaa aatatagtct tactttgctg tcatttcagt
    25381 atttaatata atttgcacat gttttttatt gtttttatca tttatcaaat aattcacctc
    25441 taaacaacaa aaacccccat cgctgggggt tataattcaa aatataatat tttgctttgg
    25501 ctgaattatt cttctttttc ttcttcatct tcttgagctt ctactacttc aacgattagg
    25561 ttagcataaa tatcatcaaa taatttaaca ggtaccttat gcacacctaa ttcctttatg
    25621 ttttcatcga gagagatatt tctcttttcg atttcataac cttcttcttc cgcagcttca
    25681 gcaatatctt tagtagtaac tgaaccgaat aacttgccgc tttcacccgc tttaacagga
    25741 aagataaatc tttcatcttc gagtttagct ttaagttctt gcgcttcttc agttctttca
    25801 gcttttcttt tcttagcacg ttctatctca tgctttaatc ttttcttttc tgattcggtt
    25861 gctctaacag catagcctct tggaatcaga tagtttctag cataaccatc ggctacttct
    25921 acgatatcgc cggaagcacc taatttatct acgtcttctc ttaaaataat ctccattata
    25981 taaaacctcc tttaacttcg ttaagcctgt tttttattat ttttaagttt cctgaaatcg
    26041 aaccaaaaat ctaccagccc gagcaagatt actgcaggtg gaaaaatcgg gataaataat
    26101 acaactgctg taaatataac tttaaataaa gaactatctg tctttttgtc aataaagtaa
    26161 atagctacag catacccctg aatcagggct aaaaacaata gtaacatatt taaatttaca
    26221 aaaaatatgt tcctctgcaa tattagggaa acaacaatac ccaatgaaat aaaccaacga
    26281 ggtaaaaacc agtactttat atttttataa gtagttcgtt tataaccagt ctttctaata
    26341 taccagtttg taacataata atttagacta cctgttataa tagcagatat acctatcagg
    26401 gccgggaata ttctctgcag caattgaatt tggctggaaa tcatatcttc cataccactc
    26461 atattaccgg tctgtccgat aatcctggtt acttcattta ttatagactg aaagtcgata
    26521 cccaacatat accgtgaaac atataaaagt aagagatggg ataccaatac agcacctata
    26581 gtaaataaca aggttcgtaa aggttttata ccttccttga tacagttacc aataacaaaa
    26641 ccaaccagac cgtaaccaat tactgccatc aaacccaaca taggtccaaa caataggccg
    26701 cttaagatag ccataattgc tattacagca gctgttgtat ttagatcata cttcaaggta
    26761 ataacagcca ccggtatcgg ccaaactact aagacaaaca cactgagaaa aggtatatat
    26821 atgttaatta aggttaaaac taatagatat attacagctt ttataccgtc ttttattttc
    26881 tgatttaaca tattatatta cacctctata tctcatcgac aaggtattgt ttaatattta
    26941 aagggaggtt atatttaaat acctcccaat taattttcaa tttattcttt tacataaggt
    27001 aataacgcta attttcttgc tcttttaatt gcttttgtaa tatctctttg gtgtttggaa
    27061 caattacctg taattctcct gggtacaatt ttacctctat ctgttatata tctatttaag
    27121 gtttttaaat ccttataatc tatttcttta tctttagcac aaaagaaaca tgatctattt
    27181 cttcctcttc ccattattaa acctccttct taaaatggta cttcaaaatc atcatctgat
    27241 aaatcttgat catccatgtt attatcttct tgctctggtt cttgtttttg ataatctgag
    27301 cggctgcttt tacctttatt attatcacta ggccagtcta aaaatttaac attatttgct
    27361 ataacttcgg gattgatata tgtcctatta tcatttttac cttggctttt tctaatttga
    27421 agtgatcctt ctacagcaac cagtctacct ttgcccagat gatgggccac attttcagcc
    27481 agcttacgcc aggttacaat cttaatgtaa tcaacttcag tttcaccctg ttgattacga
    27541 tagtttcttt ctacagctaa tgtaaaattg gcaactggag taccattact ggtatatctt
    27601 aattccggat ctctaactag ccttccaatt aatacaatat gatttaacac ttaagccacc
    27661 ccttaaatta gtcgtctttt ctaattatta atgaacgaat aatctcacct aaaatacggt
    27721 aattatgctc tagttgatct agcacatcag gtgaggaatt aaagttaata aaagcatagt
    27781 aaccagtacg ataatcttcg atctcatatg ctaactgtct gttaccccat tcatcgatat
    27841 tattaatctc gccatcatta tcggtaataa cagacttaat tctttcgata atagcttctc
    27901 ttttttcctc atccaaatct ggtcttaaaa caattgctgt ttcgtaattt ctaactttat
    27961 ccatcttttt cttcaccccc tccatacggt ctaagcggct tcatatttgt ctttatgaag
    28021 catggattca tttatatgaa tttatataac acaattcttt accattatag cattgaagta
    28081 ttgaaattgc aacttctaac gagataatat taaacattaa atttgaaata acagaagtcg
    28141 ccatcctgaa ttatataatc atgtccctcg atcctcaaca gaccctcttc tctcgcattt
    28201 ttaatagagc cggcttccat taatttttca tagctaataa cttctgcctt aataaaaccg
    28261 cgctccatat cagtatggat tttcccagcc gcctgtgggg ccttggtccc ttttttaacg
    28321 gtccaggctc ttatttcttt ttgaacggct gtaaaaaatg ttatgagatc taatagccta
    28381 taacaggatt taatcacttt atttaaaccc gattcttcta aacccaggtc atctaaaaat
    28441 aactgggctt cctcctcatc ctctagctca gctatatcgg cctcaatttt ggcacttacc
    28501 gggacaactt ccgtattatc ttcttttgca tattccctaa ttctggtcaa atattcatta
    28561 tcttccttat taatatcact ttccgaaata tttgctatgt aaataacttt tttatcggac
    28621 aacaaaaata attcatctac taattctctt tctttttggt ctaattccat atttctggcg
    28681 ggaattcctt catttagacc tttttcaacc ttctctaaaa tttcatactc ttttttatat
    28741 ttatcctcgc ctgacttcaa catccgttct gtcttttctt tccttttttc cacggtttct
    28801 aaatctgcca taattaattc cgtatttatt acctctatat ctttaaccgg atttaagtcc
    28861 ttgtccacat gagttacatt ttcatcttca aagcacctga caacctgagc tattgcatca
    28921 acttctctaa tatgggacaa gaatttattg cccaatcctt ctcccttatt agcaccttcc
    28981 acaagtccag ctatatcaac aaacttaatt gtagctggga tctttttggg tggtgaaaat
    29041 aattcataga ggtcatcaag ccttttatca ggaacttcta ctactcccac attaggttct
    29101 atagtacaaa atggatagtt ctcagctgaa actcctgctt tagttagtgc attaaataga
    29161 gtagattttc ctacatttgg tagaccaact atccctattt ccataatatc cccctatttt
    29221 ataatttcct ttagattctt ctcaaatttc cgcctgggca tcatcacaat atgattgcaa
    29281 ttttcacatt cgattttaat atccatgccg actctaataa ctttccagct gtttgagcca
    29341 cagggatgtg gttttttcat cttcacccta tcgccaactt tcaaatcagc catctaagat
    29401 cacctcgatt aagtaaggac aaaatataat attgcaagaa ttattactcc aacaaagaac
    29461 aagcaaccct tgtttttata taagggttta tcctcaaatt taaatgaata tgaacctctt
    29521 ttacgggctt tctttaagta tctaacagat tttgtatact taccctgttc tctgtatatt
    29581 acacccaaat tattataggc attaccataa tcggggtcaa tctcaattgc cttttcataa
    29641 aatctttcgg ctttatccaa atcacccttc tttttagcaa tgcttcccaa attggttata
    29701 gcgggtacat attcaggatc ttcttggaga atttcattaa gaatctcttt agctttatct
    29761 aaattatcat tataaatttc aatcacagct aacttattta aaattggtaa aaatttctct
    29821 tctacttcta aaatctcatt gaacctttct tttgcttcct caaattcacc aatatctaat
    29881 agatcagaag cctctttata ttttttttgt aattcatcat caagtctatt aatatattca
    29941 tcttctaaca tatcttatcc cctcatcttt ttattctttc aatattatat aatattaaag
    30001 agtgtaaagc aatcttatag aagaaaaagc ggcccgaagg ccgcctcata acttctttaa
    30061 ttttgccatc ctaaatctgg ttcccgtgct gctttaacct catccaactt ccggactgaa
    30121 gtattatgag gagcattttt tactacttcg ggattttctt caacttcttt agaaattgtc
    30181 tttaaggtat caataaacct atttaatgtt tctagattct ctgtttcagt cggttcaatc
    30241 atcatggctt ccttaacaac caaggggaag tagatggtag gagcatactg tccataatcc
    30301 aataagcgtt tggcaatatg gagtgtatct ccaccttttt ccttctgttt tatacctgaa
    30361 agcacaaatt catgtaaact atttgcttca taaggtaata agaattcttc ttttaattgg
    30421 cttttcatat agttcgcatt taatacagca ttttcggttg tctttttaag tccacttcca
    30481 cctagcgtct taatataggc ataagctctg attaaaacac catagttgcc gtaaaagccg
    30541 tgaattttac ctattgattt aggcctatca taatcccagc aatatttacc ttcttttttc
    30601 tgtagtacag gccgtggtag ataaggtttt aagaattcct taactcccac agtaccagaa
    30661 ccaggacctc cccctccgtg aggagtagag aatgtcttgt gtagattcaa gtgaaggaca
    30721 tcaaagtcca tgtcgcctgg ccttgcataa cccataacag cattcagatt agctccatca
    30781 taatagagta gtccacctac gccatgaatt atatctgcaa tttgttgtat atccttttca
    30841 aaaataccaa gtgtattagg attggtaagc attagagcag ctacatcgtc atcaactgct
    30901 gcttttaatt tttcaatatc taccatacct tcttcattag aatcaatttc aactatatca
    30961 aaaccggcca ttgttgcact ggcaggattt gtaccatgag ctgaatcagg cacgataacc
    31021 ttagatttat caatcccttt atcttcaaaa tatttacgaa ttattaataa accagtaaat
    31081 tctccgtgag ctccggaggc gggctgtaag gtcatctcat ccataccact tatctctgct
    31141 agatcctgtt ttagttgata gaggatctca agtgaaccct gcacatcttc ttgagcctga
    31201 taaggatgta gattagttaa tttagagatt ctggcaatat cttcattcct tttcggatta
    31261 tatttcattg tacaggaacc aagtgggtaa atccctgaat caactccata attcagttca
    31321 gataaagctg tataatgtct aactacatca acttcactta cttctggaaa gtccagctcg
    31381 tctcttatat aatcacttcc cagttgttct tctaaatcta attccggtac ttctaaagga
    31441 ggtaatgaat aaccttttct gcctgggctt gaataatcct tgataagttt ttcagccatt
    31501 tacatcgccc ccaataattg aactaattca tctaattcat ctttagtcct tttttcagtt
    31561 acactcatta atattccttc ttcttcgtca aatctggata agtctatccc acccaggatc
    31621 ttattttctt taagttcagt caatacctta gaaaccggta gtttagtttt tagccaaaac
    31681 tcatggaaat aattgtcaat attaactact tcaaacttat ctaattcgtc tattttttca
    31741 gctaaataat gagcttttgt aaaggactgt tcagccactt cttttagtcc ctgtctgccc
    31801 atggtagaca tgtagatcgt tgaaatagct gcattgagag cctcatttgt acacatatta
    31861 gaagtagctt tttctctcct tatatgttgt tccctggtct gtaaggtaag cacataacct
    31921 ctattacctt cagcatcatc agtggcacct gcaatccggc ccggcgtctg tctgataaat
    31981 ttacgatcat ctttacaggc tagaaatcct aggtaagggc ctccataatt gagagtatta
    32041 cctagactct ggccttcccc taccactata tcagcatcaa atgcaccagg tggggttaaa
    32101 gttcctaagg caattggatt tgctacaact aataataata ctctcttata ctgttctttt
    32161 aactcttgta ttttcttcat ctcttcaatg gaaccataga aattagggta ttgtacaacg
    32221 atgacagctg tatcatcatc gagattggat tccaattcag taagatctgt tatagaacca
    32281 tctaagccaa tttctacaaa ttctaaattc tgtggttccc cataagtctt tgctacctct
    32341 ttataagaag gatgaacggc actagagatt aagatttttt tatttctact gattctagat
    32401 gccatcaaga cagcttcccc catcgcggaa cctccatcta aaagggatgc attggcaaca
    32461 cccataccgg taagttcact gatcatactc tgatattcat atattacctg aagagttccc
    32521 tggcttaatt ccgcctggta aggtgtatag gcagtataaa attcctgacg ggagatcagg
    32581 tgatctatga tcccgggaat atagtgatca taggcccccg cccctaagaa agaagtataa
    32641 ttttcaagtg aattatttac ttcagatatt tcatgcatat gtctttttaa ttctaactct
    32701 gacataccct ctgccacatc taattctctc tctaatttaa tttctttcgg aatcgagtca
    32761 aatatctctt ctactgcctc cactccgatc tcttttaaca tatattctct ctcttgtgta
    32821 gtattggaga catagtccat ttactattcc tcctctagaa atgcttcata ttctgtgcta
    32881 tccatcaatt cctctaattc gtctttatct ttaatttcaa tcttaattaa ccagccactg
    32941 ccataaggat cttcattaat taattccggt ctatccatta attcttcatt tacttctact
    33001 attacaccac ttgccggcat aaatacatca gatacagcct ttacagactc aattacacca
    33061 aattcttcaa attgatctac ttcttcttct actgatggta attctacaaa tacgatatct
    33121 cctaattctt cctgggcgtg atcagtaata cccacgactg catctttccc gtctactgaa
    33181 atccattcat ggttttcagt gtaaaataaa tcgttcggaa tgctcattaa aaattccccc
    33241 tttttttaaa taaatggtgt cttaaaaatt ttcgctttaa cttctctttt ccttatacag
    33301 acatctaact cagtatcttc ctctgctagc tttttatcaa cataggcaag tccaatattt
    33361 tcatcaagtg taggtgaata actaccactg gtaacttctc ctactacttc accatcaacc
    33421 ttaatttcat aaccatgtct tggtataccg cgttcctgca taataaagcc tactaaagtt
    33481 ctcttcacac catcttcttt cactttctct aaagcttctt taccattaaa atcttcttta
    33541 tcgaatttta cagtccaacc tagattagct tctaatggat ttgtagtctc atcaatatcg
    33601 tttccatata ggcaaagtgc tttttctaat cttaaagtat ctctcgctcc taaaccagca
    33661 ggttttaagc ccttatcact accttctttt aacattgcat tccagacctg aacagcatct
    33721 tctgctttta aatatacttc aaagcccaac tcgccggtat atccggtcct ggaaacaatc
    33781 gcttcaaccc ctgcaatatc agtctctata aaggtatagt actttaagtt actgatatcc
    33841 tcctctagta aaggttgaat tatttcccgg gaaataggac cctggagtgc aattaaacca
    33901 taataatcag atttgtcttc aacttctgct tgttcaaata attcagcatt cttttcaacc
    33961 catctgaaat ctttggtagt atttgatgca ttaacaacta atagaaattt atccacatcg
    34021 atcctgtaga ctaaaagatc atctacaatc ccgccatctt gataacacat tggcgtataa
    34081 aggacatcac cattagttaa tttagaagca tcattagtaa ttatcttttg aactaaatca
    34141 agtgcctctt ttcctgaaat taatatctca cccatatgag aaacatcaaa aattcctgct
    34201 ttattcctta ctgctttgtg ttcatcaata atgccggaat attgaactgg catctgccag
    34261 cctccaaaat caatcatctt tgcgtccaaa ctttgatgtt cttcgtaaag tgcagtactt
    34321 ttcacatttt cacctcctaa ttttaataat aaaaaaagag gagcttaagt ttccttaaac
    34381 cccccctgtt ctggtaagtc cagagtttgg tcccttcaga tttcttctgc ctgagagttt
    34441 cggctctagt aatgagcttt gctccttcgg cgcctttttt caaggtctct catctgaatt
    34501 tcaacccaaa tcatattatt ttcttttttt ctattatata ttttatactt cttttgactt
    34561 tttttcaaca tttttttcat ttttcttttt atttttatct tcttttgatt gggtaaagct
    34621 ttgttttttg gtagaatttt ccaggggagc gcttttgcca atgaattttg cgccttcctc
    34681 tactaccaac tttttagcct ttatatcgcc ttctaaggtg ccagtttcaa gaatttcaag
    34741 cttatccttg gcaattatat tcccttcaac cttgccggct ataacgacct ttctggcctc
    34801 tatgttggaa tttacttcac ctgattctcc cacaaaaaca tcaccatctg cctctagagt
    34861 accagtaaga gaaccttcga ctcttatcga tccttttgtc ttaagttttc catcaaactc
    34921 agtctgttga ccaaaaatag tgctaatttc atctctttta acatcttctt ctttgttaaa
    34981 caataggatt acctcccaat aaattttctg gggtttttag gtaccccgtt tattctcact
    35041 tcataatgta ggtgggggcc agtacttttg cccgaattac cagttaaagc aataacttca
    35101 ccttctgtga ccttgtctcc tttactaact ttacttttgt ttagatgcgc ataatatgta
    35161 ctataaccat gtccgtgctc tacttttaca cttaaaccat aaccaccatt ccagccgaca
    35221 aaagtcacct tacctggagc agttgccact acaggagtgt tataccagac tgctatatct
    35281 aaaccctcgt ggtattcttg ttgatttgaa atcggatcga ttctccaacc aaaatttgaa
    35341 gatatgtaac agtttccact atcagcaagc ggccacagat tagggagcga agacctcata
    35401 tccttatatt ctaccgcttc atcttttagc tcattttgga cagatctttg ttttgaaata
    35461 gtatcttctg ctatattgat ttctgtcttc atttgttcga taagtttatc tgtttcatgg
    35521 tagtataggt ttaattcgct gccgcccatc ggaatacctt gttttaaaat attactgtta
    35581 gttctcagaa tacttgtaat attaatttct tcatcaaaat cattatcttc attgatattg
    35641 gtatcttcac ctaatatata ccttatttcc tggttggctt cctttatact ccttaaagaa
    35701 gccattaatt cttccgtgtt ttggcttaaa accagtaaat ttttcttcaa tcttttattc
    35761 tcttcctgta gttcttcgat atttacgcta tttttgaatt cttgaaagtt tgcttgggtt
    35821 tgtttatatt tccctttata ataaaggtaa ttacaggaaa gtataccaat accaataact
    35881 aataataata tgatgataat aggaataaca cgtctaaaag agaagttttt gattttatta
    35941 cgcgtagaag aaggggttac tgttatgtag agtttttcgc ttagaaattc tttgatgttc
    36001 ttccaaagcg aaccattcat aaaattcctc cccttactat atttgtagtt atttgctcaa
    36061 attagattat atcataaaat ttggattaag aaaagtatat ctaattatta aatttaaaat
    36121 acaaactggg ctataacaat tgccacaaag attgaaggta ataagttgcc aatctttatt
    36181 tcagcggaaa gaagcatatt aagcccaatt gccataatca aaagtcctcc cgtagctgtc
    36241 atcatggcga caacatcctg gttgagaagc ttctgtgccc aggatgccaa taatgaaata
    36301 gaaccctggt ataaaaggac cggaatacta gaaaacatga ctccaattcc aaatgtggct
    36361 gaaaaggcca cggaagctgt tccatctaat aaggattttg taaataagat agacggatca
    36421 ttatttaatc catcctgaat tgcgcccatg attgccatgg cacccacgca gtaaatcaag
    36481 ctgctctgca caaacccctg tacaaataag ccatcatcac tgccaaatcg ttcctggatc
    36541 ttttctccaa acccttctaa tctactttct acttttaaga tttctcctat tattccaccg
    36601 attactagac tgaaaattac taccagcgtc cctttggtgc tttctactga aaaagccatc
    36661 tggagaccga tgattaaaac tgccagactt atcccctgaa taactatatt ttgaaatctc
    36721 tccggcagtc ttccttttac taggaatcct actaagcttc ctattaatat cgctattaca
    36781 ttaactattg tgcctatcat atcaattaac ctccgttaaa cattttaatt aattcttctt
    36841 tatactccct cctcgtcaga gcaactatta gatcatcttt tttaataata gagtctccac
    36901 gcggaactaa gctttcatcc cctcttaaga ttgtaactaa aaccgattct aaggggaagt
    36961 ctatctccct taatttttta tttaaataat cacagttgtc accaactgga attctcacta
    37021 attctatttt gtcatggttc ttatttaaaa tgtatctaag atcctgtaag gtcatttcct
    37081 ggtctacgag agccgtcaaa atagaagcac tgcttacagc cacattaact cccaaccagt
    37141 cgaaaagcct ttcattgccc ggggtattaa ctctggtgaa tgtccgcgga acatcgaatt
    37201 tacgttcggc catctgacaa ataacaagat tatcttgatc atcacctgtc acagctaaaa
    37261 ctacatcagc ttcatttata cccgcttctt ctagtaaatt ttcattagtg ccatctccac
    37321 agaatacttt tactccatat ttttgtttca accgtttaca gttatctgtg ttattctcta
    37381 ctgtatgtac tttgtgatct ttctctaata attccttgat tagataagat ccgaccttgc
    37441 cgcctccaac tactattata aacatttttt cacctcaatt agtaattgtt tcacgtgaaa
    37501 cattatctgc tattaactca acttgtcaat tatcttttcc atctctttat aatctttaca
    37561 atttattgta acaacttttt tgcttacttt atccttgatg tcaacatcaa gccccagtct
    37621 ttcagctaac agctctcttg ccttgaccca tttgggcgct aactcttttt tattcttttt
    37681 agttttctct tttttatctt tattctgata tttttttact agttcttctg tttttctaac
    37741 agatagatct tcttcaacta ttttatttgc caaatccacc tgttttttgc tttccagagg
    37801 taataatgct ctggcatgtc ccattgaaat tgtttcacgt gaaacaattt cctggacaga
    37861 ggggggtaaa tttaacaatc ttaatgtatt tgcaattgct gaccggctct ttgataccct
    37921 ttctgccact tctttttggg taatatcaaa ttcttcgatc attttttgat aggcataggc
    37981 ttcttcaatc ggattaagat cttccctttg taggttctca attaaagcca cttccatcat
    38041 ctcatcatct ttgaactccc tgatgatggc tggaattgta tcaagcccgg cctttttagc
    38101 ggcccgccat cttctctcac cagttacaag ttcaaaattg tcatattgaa cttctctgac
    38161 tgtgatcggc tgcagaactc ctttgttcgc aatggagtta gccagctcat caagcgattc
    38221 ttctgtaaaa cgatctctgg gttggtaagg atttggctta atcttttcta tatctatatc
    38281 ttttatttcg tgttctctct tttcaccctg actttttgat agaagtgcgt ctaacccttt
    38341 tcctaatctt ttcttattca cgtgcaatca catcctttgg taattcttta taggcatgag
    38401 ctccccggga actgggatca taagtaataa ttggttcccc aaagctgggg gcctcgctaa
    38461 gccttacatt cctgggaatt atagtgtcaa aaacatcttt tccgaagtat tttttgactt
    38521 cttcaatgac ctgacttgaa agattagtcc gggcatcatt cattgttaaa acaacaccta
    38581 ataaggttaa atcagggttt aagttgctct gtactaattc tatagtttgc atcagttggc
    38641 ccaatccttc taaggcataa tattcacact gaatgggaac aatcaatgaa tcagaagcag
    38701 ttaaagcatt taaagttagt aaaccgaggg aaggggggca gtcaaatatt atataatcat
    38761 attctatatc agattcttca atcttctttt ttaagcggct ctccctggac ataactgaaa
    38821 ctaattcaat ttcagcccca gcaagatcta tattcgcagg aagtatatag aagttgtgat
    38881 gacctgtcgg ttgaataata tcatttagat cagtgtcgtt aattaaagca tcgtaaatac
    38941 tatattcaag atcacttttt tctaacccca atccactggt agcattaccc tggggatcaa
    39001 catctactat taatacttcc cgccctcttt ctgcaatgct ggcccctaag ttgatagctg
    39061 tggtgctttt accgacacca cctttttgat taactatagc tattttcctt gccaagtaaa
    39121 aaaccccctc atttattttc ttcctgcttt tttggtattt cgatattata ccttatatat
    39181 tccttatcat cttgtttgtc tacctttact tcaattccgg ccttcctcaa ctgatctata
    39241 gcagaattta aggtgttttt taacaacctc atatctgcat aataagtttt tacattttta
    39301 ctatctggct ctttttgttt tttaagatat ttatctatct tcctgtttaa acccctaaca
    39361 gaaagatctt tttctttcgc ttcttttata aattcctttt gtttttcatt atcatccact
    39421 tttaggaggg cacgggcatg tctttccgat aaattatact tcctgattaa ggctaaaatt
    39481 tcttccggta gttttaatat tcttaatttg ttggcgatag tgccctggct tttcccaact
    39541 ttatcagcca tctccgtttg actaagatca aatttgttca taatactgta ataagctagg
    39601 gcttcgtcta aaaagttgag atcctgtctc tgtatatttt ccaccagcga tatttcagca
    39661 acttcctctt ccttataatt gtttacaatg gccgggacct gctctaaatc aattaactta
    39721 gcagccctca gccttctttc gccggcgatt agttgatact tgccgtcttt ttcactcact
    39781 attaaaggct gaataatccc gaagttctcg attgattcag ctaatttctg taagttcttt
    39841 tcactaaatt cagacctagg ctgatgggga ttgggtacta ttttatcggt ttttattttt
    39901 tctattttat tattctggct ctttttgcct aaaaaaggaa agtccattta tttccctcca
    39961 ttacttttat aattattctt tataatcaat acttttcctg cctaaagagg gcgtttttta
    40021 ggaattcccg cccgcctggg gtacttatct gaaagttcat tttttctcac gaattctaag
    40081 atatatctag ttccatctag aaaagggata tctaatttat agctattcat atatttcaaa
    40141 tttaatttct ggattgcttt gtgggcctct tttaattcat cttggtaaga aggtccctta
    40201 tataatagga ttttaccctc gttcttacag aaaggagctg tatattccaa taatatatta
    40261 aggggggcca cggcccggga agttacataa tcatattgaa atctgtaatc cttgttttgg
    40321 cctaaatctt ccgctcttgc atgtaaaacc tctatatctt cataaagttc taattctacc
    40381 tgcagcattt ttaagaaatt agcctttttt aagctagctt ctaaaagagt tagcttaaca
    40441 tcgggtttta aaattttaaa aacaaaaccg gggaaaccag ccccggtgcc aatatcaatt
    40501 accttacctc caatatcgct tttgagaaag agtccagccg aatccaaaaa atggaccttg
    40561 acaatttctt ttggctctgt aatagaagta aggttatatt ttttattttc ttgaattagg
    40621 aagtctatat actttaaaat ctgctctttt tggtttttat ttaattctaa acctatttgt
    40681 ttaaaacccc tatctaaata atctttcagg ctgtttttat tcattttcct gttcgctttc
    40741 attagatttt tgctcgcgtt cctgctgttc aatatagatc atcagtactg atatatcagc
    40801 aggagagacc cctgagatcc gggaagcctg tcctaaagat ctcggtcgta ttttatccaa
    40861 cttttctcta gcttccagtc ttaagttttc taattcatca tagttaatgt cttctggaat
    40921 taatttgtct tccatcttct taaactgttc aatctgagat tcttgccggt caatatatcc
    40981 tttatactta gtttggatag taacctgttc tttaatttct tggcttactt ctggtagatt
    41041 gggagcaaag aatttaagat catcatattc aacttccggc cttttaagca gtttggccag
    41101 cttaacgggc tttttaagag caccgctgtc taggcttttt aatctttccc tgttttccgg
    41161 ggtaggagta acttgaacat ctcttaaaaa ttccagggtg cgttcaatat catgtttttt
    41221 attcaaaaac ttctgataac gttcttctga aattagtcct aaattatagc cgatttcttt
    41281 caacctaaga tcagcattat cctgccttaa aattaaacga tattcagccc gggaagtcat
    41341 tattctataa ggttcctctg tacccttggt cactaaatca tcaattaaga ctcctatata
    41401 agcctgagaa cgatctaata tcaatggctc ttcacctttt aatgataaag ctgcatttat
    41461 gccggcaata attccctggc cgccggcttc ttcataaccg gaagtcccat taatctgtcc
    41521 ggccgaatag aggccgttaa tccttttcgt ttcaaggctt aactgcagtt cactggggtc
    41581 aagacagtca tactcaattg cataagctgc cctcattatc tcagcttttt ccaagcccgg
    41641 aattgttttt agtatctcat actgaacatc ttccggtaga cttgtcggta ggcctgaaat
    41701 ataatattca ccggttaatt caccttccgg ttctaaaaat aattgatgac ggtctttatc
    41761 agggaatcta acaactttat cttcgataga aggacagtat ctaggaccga ccccttcaat
    41821 aacaccacta aataggggag tcctcatctt attatcacgg ataatcttgt gagtttcttc
    41881 atttgtatat gtcaaataac aggaaacctg atctctctca agcggaggag agtcaaaaga
    41941 aaaggaaaga ccttcatctc cggtctgttc atgcatcttt gaaaaatcta tactcctttt
    42001 attaacacgc gggggagtcc cggttttaaa ccttcttaag ttaaaaccta aatctttaag
    42061 tgattcagat aacttattag caggatactg ctgattaggg cctgaattaa aggtcgcttc
    42121 ccctataatc actttacctt taaggaaagt accggtagtc attacaacct tttttgtctt
    42181 gaataatatt ccagttttgc taataactcc ctttatttct ccctcttcca ctacaagggt
    42241 ctcagcaatc tcctgtttta aatcaagatg ttcttgttct tcaagaacct gcttcatacg
    42301 agtatggtat tttttcttat cggcctgacc tcttaagccg tgtacagccg gacctttcgc
    42361 cgtattcagc attctaattt gcagcatggt ctcatccatg ttaattgcca tttctccgcc
    42421 taaggcatca atctctctta cgatatgtcc tttacccggt ccccctaaag aagggttaca
    42481 gggcataaag gcaacatgat caagattaac ggtcattgtt aaaacatcaa aacccatgcg
    42541 ggccggggcc aaagaagcct cacagccggc atgtccggca ccaatgacaa ttatatcata
    42601 ctctttttca aacactttat tataattgta attattttcc atttttataa aacactcctt
    42661 acctatttac ccaaacagaa gtcactgaaa atctggtcta taatatcatc agtaacggtt
    42721 tcacctgtaa tcgtgcctag atagaagaga acatccttta aatcaattgt taaaaagtca
    42781 tggggcattc cctcatctaa ggactgcttg acctttctaa tcgaatctaa ggccttatta
    42841 agagcatccc gatgacggac cttagtaacc ataacatcac tatcaacatt aatctgttca
    42901 tgtaagatct cttctaaaat agtatttttt aattcttcga taccttcttc ctctttaaca
    42961 gaaatccaga ggagaggatg ttccttaaat tcttttttca tttcttctaa atcaattgac
    43021 tgctctaaat cagttttatt aattacaata atcgtaggtt gctcttttat taattcataa
    43081 acttttaaat cttcctcagt taagccctga ctgacatcca gcatcataat tactaggtca
    43141 gccttttgga gagaattcct ggttttctca actccgatct gttctacaac atcatcagat
    43201 tctcttactc ccgcggtgtc tataattttt aaggggatcc catctaagtt aatatactct
    43261 tcaattacat cccgggttgt gcccggcaca tcagtaacaa ttgccctatt ttcctgaagg
    43321 aaataattga gcaaactgga tttacctaca ttaggttttc ccacaataac agtttttacc
    43381 ccttcctgat aaagcttacc ctgcttggca gttttgatca gtttttcaat cggcttttcg
    43441 atatttttta ctttatcttc taattgagaa gaatcaaatc cctcgatctc atcttctgga
    43501 aaatctatgg aagcttccag atgggctaat aatgtgatca cctcatcttt aatatcatta
    43561 accttttcag atagtccgcc ttttaactgt tccatcgcta ttgaaaggct tttttctgtt
    43621 ttggaattta ttacatcaat aatagcttcc gcctgggcca tgtctagttt tccattcata
    43681 aaagcccggc gtgaaaattc gcccggttcg gccattcttg ctccctgatc taagaccaat
    43741 tccaggactg attctaaggg aaccatacca ccgtggcagt caaattcaac cgtattttct
    43801 ccggtaaagg atttggggtg aaccattatt atagccaata cttcatcaat ggtgtcttta
    43861 gtggctggat ctactacata accatggtgg gcggtatgag aatcggcttt ggtcaaac
//
LOCUS       DATGCJ010000116        12601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_147711, whole genome shotgun sequence.
ACCESSION   DATGCJ010000116 DATGCJ010000000
VERSION     DATGCJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147711"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VKN64_05780"
     CDS             <1..90
                     /locus_tag="VKN64_05780"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMA59786.1"
                     /translation="LLEPVTGNTDYNHLSVRSAASIIMDRLLS"
     gene            132..506
                     /gene="rplS"
                     /locus_tag="VKN64_05785"
     CDS             132..506
                     /gene="rplS"
                     /locus_tag="VKN64_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMA59787.1"
                     /translation="MNIIDNINKENMRDDIPEFIAGDNVRLQIKVVEGGKERLQQFEG
                     VVISRKGSGITETFTVRKFSHGVGVERTFPVHSPRIQNFEVIRKGDVRRAKLYYLRER
                     KGKKARIKEKKAKREQSEEREI"
     gene            569..1429
                     /gene="ylqF"
                     /locus_tag="VKN64_05790"
     CDS             569..1429
                     /gene="ylqF"
                     /locus_tag="VKN64_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HMA59788.1"
                     /translation="MNIYPGHMAKAKRVLKENLKNVDLVVEVLDARIPYASHNKQLKE
                     IIENKEKIIVLNKKDLAEQKYTDEWQNTIEKTVNCVKVNAVKKSGIKKLINLMEEKQT
                     EINQKRRQKGIKERPVRAIIIGIPNVGKSALINAIGGRSSAKTGNTPGVTRGKQWIKV
                     TDKIELLDMPGILFPKAEDEDIAYKLVLTGAIALKKVDTELAAYKLLKYLRQLERLSV
                     VEKTYDIEIYQGEHPYDILPKIGRVRGCLMSGGKVDRNRAAKLLVNDYQNGKLGRVSL
                     EKPGSDNFEF"
     gene            1410..2213
                     /locus_tag="VKN64_05795"
     CDS             1410..2213
                     /locus_tag="VKN64_05795"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMA59789.1"
                     /translation="MIILNFEEMTIKEIKNFINEFENIEKIDNGIIEKLNSDSRKGVN
                     KIAARIEKQKEKKQEKIIQWHQQNNHLYNLKKEGYQLVAGIDEAGRGPLAGPVVSSAV
                     ILDSKEPILGLTDSKKLSLKERERLFDIILAKAVAVGLGIVSNTIIDRLNIHQATFLA
                     MRRAVLDLEKQPEYLLIDGSYTIPDINIQQKAIVSGDLKVNSISAASIIAKVTRDRIM
                     DELHNSYPKYNFLSNKGYGTKEHIDVIKESGPCPYHRFSYKVVDKNKSE"
     gene            2219..2686
                     /locus_tag="VKN64_05800"
     CDS             2219..2686
                     /locus_tag="VKN64_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HMA59790.1"
                     /translation="MNTKYLTRGALIAGLYVIITYILSPVSFGPLQFRAAEALTVLPI
                     LFPEAIPALFLGVLLSNIIGGLGMVDIIGGSLVTLLAAYGTYYFRGTFLAYLSPIVLN
                     GFLISIYLHLLFEIPYWVTVVQISISEAVVVLLLGYPLIQVLKKRFDFNQHNL"
     gene            2757..3278
                     /locus_tag="VKN64_05805"
     CDS             2757..3278
                     /locus_tag="VKN64_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMA59791.1"
                     /translation="MLSEKMEEQLNEQINAEIYSGYLYYAMEAYFEDEDLTGFANWMH
                     IQAQEELLHAQKFFNFINERGGRVSLDAIEKPKADWDSPLEAFKDAYEHETKVTDRIN
                     KLYDLAVKEKDHATASFLNWFIDEQVEEEASADEIVNKLKRIGESGQGIFMLDRELGS
                     RTLDTAEYAEESE"
     gene            3377..3685
                     /locus_tag="VKN64_05810"
     CDS             3377..3685
                     /locus_tag="VKN64_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59792.1"
                     /translation="MGLFDKIKDFFTPSSDSKIVKLYVKDKKCGNKMRVVLRKGYDIQ
                     RIYNDEVEGKYKINKVIVCDKCYNNVVIDVTFDKNYKIINQSIENGEFITEEEFLKDQ
                     "
     gene            3702..4211
                     /locus_tag="VKN64_05815"
     CDS             3702..4211
                     /locus_tag="VKN64_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMA59793.1"
                     /translation="MLLNYPNTLTVVRLLLIPVYLYFFLQGEYILSGVFFSLSGVTDF
                     LDGYLARKYNMVTDLGRLLDPLADKLTLISILAVLIYMDLLPRVITITLLTREVFVLF
                     GSGIMYLMGKDLINPTRLGKISIFLLYVAIAASLLDIKFFDMLLFYVVIPINIISAVD
                     YIRNAYQKM"
     gene            4228..5511
                     /locus_tag="VKN64_05820"
     CDS             4228..5511
                     /locus_tag="VKN64_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA59794.1"
                     /translation="MNILLKNIKEIYSPSQDLSEYKHIEIEGKEIKRVIKDDDIVSND
                     HDRVIDCSDMVVLPGLINTHTHSSMSLLRGYADDFKLDKWLSDNIWPAEAKLTREDIY
                     WGAMLAVVEMIKTGTTTFTDMYFQMDKVAEVVKKSGIRAVLSEGLIEDNDGMEGLNDS
                     VDFALKWQNKADGRIKTTLGPHSPYTCSADYLEKIRDKALANNLLIHVHISETDDELG
                     FMKDKYGLSTVELFDKINLFDTKVIAPHCVHLSESDIEILADKDVGVVYNPASNMKLS
                     SGIAPIKKIMDKGINVSYGTDSAASNNNLDLIEEARLGSYLQKVATGDPTVLPIEENM
                     KMLTVNGAKTLGIPNLGEIKEGYLADLVLINVKNTAHQYPSYNYLSNIFYAGNGCDVN
                     TVIVNGEIVMENRVLIKINEEKVYKKVEQLTQKFR"
     gene            5536..8292
                     /locus_tag="VKN64_05825"
     CDS             5536..8292
                     /locus_tag="VKN64_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HMA59795.1"
                     /translation="MKKGFRYLFSIIFVVFIALLIAIFAGANFFTEWAWFNQLGYLKA
                     FLLMFFSNFGLRVLIGVIFAVFVYINLSFTKKLFYKYFKKEKVDSENVETLFKEEGQG
                     FLNWLDKRKLNWLYIIISLVLGFLFSSVSSELWKIVLKYFNQTPFGTTDPIFGRDIAF
                     YVFSLPFYSFIKEMGMVLIVLTIIVVGVIYFIASGISSFSEVKVKLSNRAKGHITILL
                     SAFLLLKAWDYRLAMYELLYSSRGVAFGASYTDINANLLGLRILFVIAIAVALGLLVS
                     LFRKNYKLLAWGLGIWIVTSLIFGGIYPGFVQRFQVEPNEINRERPYINHNIQMTLDA
                     YDLNDIQTTDFEVEDGLTYEDLQENDEIVKNIRLWDHRPLLTTYNQIQTLRQYYDFQN
                     IDVDRYNIGGDYRQVMLGAREMNQDLLSGQAQTWINKKLKYTHGYGVTMSPVNMVTSE
                     GLPEFFMSDIPTQVNVDIPLENAAIYYGEKTDEYVIANTDSTEFHYPQGDDNVYINYD
                     GTGGVLAGSFIKKAVFALRFNELKFLLNDDINPDSRVMYHRNIKERVRRVAPFLQYDG
                     DPYLVVSDGRLFWIQDAYTTTDRYPYSQPVRGVGNYIRNSIKVVIDAYNGTMNYYVVD
                     DEDPIAKTYQKIFPDVFLDGEEMPEDIRDHIRYPKDLFSIQTQVYATYHMQDPVVFYN
                     KEDLWNIPTENYAGSSIQMEPYYITSKLPGNDKLEFILMLPFTPANRNNMISWIAGRS
                     DGENYGELLEYQFPKDTLVFGPNQIESRIDQDSEISQRLSLWSQRGSNVIRGNLIVYP
                     IERSILYVEPIYLQAESGEIPELKRIVVSYGGEIVMDETLDGALAKIFGEKPQEEVPE
                     EVPQSQQMLPEDMQGLAQRAYEIFQEAQQAQQEGNWSEYGNLLEELNNVLNELNDLSQ
                     QTAE"
     gene            8310..9599
                     /locus_tag="VKN64_05830"
     CDS             8310..9599
                     /locus_tag="VKN64_05830"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMA59796.1"
                     /translation="MLKTSKFGEYKTENDIDVYINNTDKFKTNYIHIDIIRPLSNEKE
                     VSQNALIPYILYRGSQKYPTNQSISHRLDDLYGADLNVSVARRGENQLLRFSVEVIND
                     KYVEAKESLIKKAIDFLYELIFRPLVEKGDFKEEYINKGQSFLKEKILSLKNDKGSYV
                     IERCYQEMCRGEDFSLYTLGTISGLEQSRQDNLYQYWQDILIESPIELFVIGDIGDSD
                     GLYKYINQKFTDHRDKKLPLNNTFIKTKDFKLKELEEEDEITQSRFCAGIRIPITRSH
                     PLYFALIVYNGMLGGFSHSKLFTTVREKRGLAYYINTRLESTKGLILINAGIDMVDYP
                     IVKEIIEEKIEEMQNGEFEEENIEWSKKKLINRYKTLSDNNHSIVDAFLLGLINGKKD
                     SLNYVINSIKQVKREDIIKVANQVNLTTYYLLRPGSA"
     gene            9602..10888
                     /locus_tag="VKN64_05835"
     CDS             9602..10888
                     /locus_tag="VKN64_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMA59797.1"
                     /translation="MIYKNFSNIGEEYYEKKLDSGLNVVLIPREKFNKKHAILSVDVG
                     SVDLKIKDNEENVYEYPKGIAHFMEHILFENREGSIIDKFSQIGSSVNAYTGFNNTNY
                     YFNTTSNFFNSLKYLFGFVLNPHFDDERIKHEKSVINQEINMYQDSPSWQSFYQLLQS
                     LFYEYPIKYNIAGTQKSIEKINKKTLIDFFEKLYNLNNMTLIVIGAVQPDKLFNWLEK
                     EKNITKQGNKKFVLEKIYPKEPNGIRLEEKDIAMSISRPIISLGYKENRCFQNKDKII
                     KQDLMTNMILEMIFGKSSDHYHALYNQDLIDENFSFNYMREKNTGFAKIYAETNSVDK
                     TISGITDIINNWEREINSEDFEVVKHKYIGRYFRSLNSFETLSSQIMKYLNLDQNYLD
                     IIQMIKDISLKEVREQYKNLLEEKEHVKIIIRTVHK"
     gene            11080..12567
                     /locus_tag="VKN64_05840"
     CDS             11080..12567
                     /locus_tag="VKN64_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HMA59798.1"
                     /translation="MQRDRWSNRAAFVLAAIGSAAGLGNAWRFPYMAYSNGGGAFLIP
                     YFVALLTAGVPLIVLEFAMGQKTQRGAPGALAKVGRKFEGFGWWAVLSGTIITSYYAG
                     VMAWAWDYLAGSFTLGWAENASDYFYNEVLQITSGPGVLGGFSVPVLIGLVITWIVIY
                     LILRKGAESVGKVVLITVPLPVLLMIILMLRAITLPGAMEGINYFLEPNFSMLTNLSV
                     WIDAYSQVFFTLSLGFGIMIAYASYMPEDSDIINNALITVFANSGISFLAGIAVFGTL
                     GYMAQTQGVPISEVATAGVGLAFVVYPNAISLLPGGSFVVGFFGFVFFAMLLSLGIDS
                     AFSIVEAGVAALKDKWGGTKRTVTLFYSIFLFVVGLLFATRAGLYWLDIVDHYVNTYG
                     LVVAGLAESLIIGWFYDPNELRGYFNSVSEYQFGSWWNVMIKYVTPIMLVVLLVNEFI
                     ADIGTAYGGYELRYLIYGGWAGVIGLLLVASFILSRQRGLDDAEI"
BASE COUNT         4332 a         1714 c         2565 g         3990 t
ORIGIN      
        1 cttttagaac cggttacagg taatacagat tataatcatc tttcggtcag aagcgctgct
       61 tctataatta tggataggct tctgtcctaa aaagtatatt ccaatatatg ttactgaaag
      121 gaggtagaca aatgaatatt attgataata taaataagga gaacatgaga gacgatatcc
      181 cagaattcat tgctggagat aatgttcgtt tacagattaa agtcgtggaa ggcggcaaag
      241 aaagattaca gcagttcgaa ggtgtagtta tttcccgtaa aggcagtggg attactgaaa
      301 cctttacagt tagaaagttt tcccacgggg taggggtcga aagaactttc ccggtccatt
      361 cacctaggat tcaaaatttt gaagtaatca gaaaaggtga tgtaagaaga gctaaattat
      421 attacttaag agaaagaaaa ggtaaaaaag ctcgcatcaa agaaaagaaa gcaaaaagag
      481 agcaaagcga agaaagagag atataatgac tttaaaaggg tgagggattt tatccagcac
      541 cctttattta ttattggagg cagtaaatat gaatatctat cccggccata tggctaaggc
      601 taaaagggta ttaaaagaaa atttgaagaa tgtagacctc gtggtggaag tattagatgc
      661 gcgtattccc tatgctagtc ataataaaca gttaaaagaa attattgaga acaaggaaaa
      721 aattattgta ttaaataaaa aggatttggc cgagcaaaaa tatacggatg aatggcaaaa
      781 cacaattgaa aaaactgtca attgtgtaaa ggtaaatgct gtaaaaaaat ctggtattaa
      841 aaagttaatt aatttaatgg aagaaaaaca gaccgagatt aatcaaaaaa gaaggcaaaa
      901 aggcatcaag gaaagaccag taagagccat cattattggg attccgaatg tggggaagtc
      961 tgctttaatt aatgctattg ggggaagatc atctgctaaa actggtaata cacccggtgt
     1021 aaccaggggt aaacagtgga ttaaagtgac cgataaaatt gagcttttag atatgcccgg
     1081 tattttattt ccgaaagcag aggatgaaga tattgcctat aagctggtct taaccggagc
     1141 catagcttta aaaaaagtgg atacagagtt ggccgcttac aaactattaa agtacctgcg
     1201 tcagttagag cggttaagtg tcgttgagaa aacttatgat attgaaatat atcaggggga
     1261 acatccctat gatattcttc ccaagatcgg gagggtaagg ggatgtttga tgagcggtgg
     1321 taaggttgat agaaataggg ctgcaaagct tttggtcaat gattatcaga atggtaaatt
     1381 aggaagagtt tcacttgaaa aaccgggaag tgataatttt gaattttgaa gagatgacga
     1441 taaaagagat caagaatttt attaatgagt ttgagaatat tgaaaagatc gataatggta
     1501 tcattgaaaa gttaaatagc gatagccgca agggtgtcaa taagattgcc gctcggatag
     1561 aaaaacaaaa agaaaaaaaa caggaaaaga taattcagtg gcaccagcag aataaccatc
     1621 tatataacct aaaaaaggaa ggttatcaac tagtggctgg tattgatgaa gccgggcgcg
     1681 gcccgttagc cggtcctgtg gtgtcttcag ctgtcatttt agattcaaaa gaacctattt
     1741 taggtttaac agattctaag aaattaagtt taaaagaaag agaacggctt tttgatatca
     1801 tattagccaa agcggtggca gtcgggttag ggattgtaag taatactatt attgatcgtt
     1861 tgaatattca tcaggctaca ttcctggcga tgagaagggc tgttttagat ttagaaaaac
     1921 agccggagta cttattgata gacggcagtt atacaatacc ggatataaat attcagcaaa
     1981 aagcaattgt aagtggagat ttaaaagtaa attcgatcag tgcagcttct attattgcta
     2041 aagtaaccag agatcggatt atggatgaat tacataatag ttatccaaag tataattttt
     2101 taagtaataa aggatatggt actaaagaac atattgatgt aatcaaagaa tctgggccct
     2161 gtccttacca tagattttcc tataaagtag tcgacaaaaa taaaagtgag tgattttgat
     2221 gaatactaag tatttaacta ggggagcctt aatagcaggc ctgtatgtta taattaccta
     2281 tattttatca cctgttagct ttggtccctt acagttcaga gccgctgagg ccttaactgt
     2341 tcttcccatc ttatttccgg aagcaatccc cgctttattt ctgggtgtat tactttctaa
     2401 tattatcgga ggtttgggga tggtagatat tatcggaggc agtttagtaa ctttattagc
     2461 tgcttatggg acctattatt tccggggaac ttttttagct tacctaagcc caattgtatt
     2521 aaatggattt ttaatcagta tctatttaca ccttttattt gaaataccct attgggttac
     2581 tgtagtacaa atttcaatca gtgaagcagt agttgtactg cttttaggtt accctttaat
     2641 acaggtctta aaaaagcgtt ttgacttcaa tcagcacaat ttgtaaatct tgcaataaat
     2701 aacaaattaa gttataattt aagtatgaat aatttaatat agtaggaggg atattaatgt
     2761 taagtgaaaa gatggaagag cagcttaatg aacaaataaa tgcagagatc tactctggtt
     2821 acctctatta tgcaatggaa gcatactttg aagatgaaga tttaactggt tttgcaaact
     2881 ggatgcatat acaggcacag gaagagttac tacacgccca aaaattcttt aatttcatta
     2941 atgaaagagg tggacgtgta agtttagatg caattgaaaa acctaaagca gattgggact
     3001 ctcctctgga agcatttaaa gatgcttatg aacatgaaac aaaagttact gacaggatta
     3061 ataaattata tgaccttgca gttaaagaaa aagatcatgc aactgcttct ttcctcaact
     3121 ggtttatcga tgaacaggtg gaagaagaag cttcagctga tgaaatagta aataaattaa
     3181 aaagaattgg cgaatccggc caaggaatat tcatgctgga tagagaatta ggtagtagaa
     3241 ctctagatac agctgaatat gcagaggaaa gtgaataaag atataataaa attcagtata
     3301 tgtcatgagt ttcaattgag gaactcatgg catatttttg tataaattaa atccctatta
     3361 tctggaggta tttaagatgg gcttatttga taaaatcaaa gattttttta ctccatcctc
     3421 ggattcaaag atcgttaaac tatatgtaaa agataaaaaa tgcggtaata agatgcgggt
     3481 ggtattgagg aagggttatg atattcagag aatttacaat gatgaggttg agggcaagta
     3541 taaaattaat aaagtcatag tttgtgataa gtgttataac aatgtagtta tagatgttac
     3601 atttgataaa aattataaga taattaatca gagtattgaa aatggtgagt ttattaccga
     3661 agaagagttt ttaaaagatc aatagttttg tggaggtttc tatgctatta aattatccga
     3721 atacattaac cgtagtcaga ttactattaa ttcctgttta tttatatttc tttttacagg
     3781 gagaatatat attaagcgga gtattttttt cgctttctgg tgtaacagac tttctagatg
     3841 gttatctggc tagaaaatac aatatggtaa cagatttagg tcgattactt gatcccttgg
     3901 ctgacaagct caccctgatt agtatattag cggttttaat ttacatggat ttattaccca
     3961 gggttattac tatcacttta ctgaccaggg aagtatttgt tttatttggc tctggtatta
     4021 tgtatcttat gggtaaagac ttaatcaatc ctacccgctt gggtaaaata tctattttct
     4081 tattatatgt ggctattgca gctagcttat tagatattaa attttttgat atgctcttat
     4141 tttatgttgt aatacctatt aatattatct ctgctgtaga ttatattcgc aacgcctacc
     4201 aaaaaatgta attataagga gattaatatg aatatattat taaaaaatat taaagagatc
     4261 tatagtcctt cccaagatct cagtgagtat aagcatatag aaatagaggg taaggagatt
     4321 aaaagagtta tcaaagatga tgatattgtc agtaatgatc atgatcgtgt aatagactgt
     4381 agtgatatgg ttgtattgcc cgggttaata aatacccata cccatagttc gatgtccctt
     4441 ttgagaggtt atgctgacga ttttaagctg gataagtggc ttagtgataa tatctggccg
     4501 gccgaagcaa aactgacaag agaggatatc tattggggtg ctatgctggc agtggtagag
     4561 atgatcaaga cagggactac cacctttaca gatatgtatt ttcaaatgga taaggtagca
     4621 gaagtcgtta aaaaatcagg catcagggcc gttttaagtg aaggattaat cgaagataat
     4681 gacggaatgg agggtttaaa cgattcggtt gattttgctt taaaatggca aaataaggct
     4741 gacggtagaa ttaaaacaac ccttgggcct cactcacctt atacctgtag tgccgattac
     4801 ttagaaaaga taagagataa agcccttgct aacaatttat taatacatgt ccatatcagt
     4861 gaaacagatg atgaactagg ctttatgaaa gataaatatg gtttaagtac agtggaatta
     4921 ttcgataaga ttaatttatt tgatacaaaa gtaattgctc cccattgtgt acatctaagt
     4981 gagagcgata ttgaaatact ggctgataaa gatgtgggag ttgtttataa tccagccagt
     5041 aatatgaaat tgagcagtgg gattgctcct attaaaaaaa ttatggataa aggtataaat
     5101 gtttcttatg gaaccgatag tgcggccagc aataataatt tagatttaat agaggaagcc
     5161 cgcctcggtt cttatctaca aaaagtagca accggtgatc cgacggtttt acccattgaa
     5221 gagaatatga aaatgctaac tgttaatggg gctaagactt taggaatacc gaacttagga
     5281 gagatcaagg aaggttattt agcggattta gtattaatta atgtgaaaaa tacagcccat
     5341 cagtatcctt cctataatta cctatctaat atattttatg ctggcaatgg atgtgatgtg
     5401 aatactgtta ttgtaaatgg tgagatagtt atggaaaatc gtgtattaat caagataaat
     5461 gaagaaaaag tatataaaaa agtagaacag ttaacacaga aatttcgtta atataataga
     5521 agggagtggc tttaagtgaa aaaaggtttt agatatttat tctcaataat atttgttgtt
     5581 tttattgctt tattaatagc catatttgcc ggtgctaact tttttacaga atgggcctgg
     5641 tttaatcaac ttggttatct aaaagcgttt ttattgatgt ttttctctaa ttttggttta
     5701 agagtattga taggtgttat ctttgccgtt tttgtttata ttaacctttc ctttacaaag
     5761 aagttatttt ataagtattt caaaaaagaa aaggtggaca gtgaaaatgt agaaacctta
     5821 tttaaagaag aagggcaggg ttttttaaac tggttagata aaaggaagtt aaactggtta
     5881 tatataatta tcagtttagt cttaggattc ttattcagtt cggttagttc agagctctgg
     5941 aagatagttc ttaagtattt caaccagacc ccgtttggaa ctactgatcc aatctttggc
     6001 agagatattg ctttttatgt attcagcctc ccattttaca gcttcattaa agaaatgggt
     6061 atggtcttaa tcgtcctgac aataattgtc gttggtgtaa tctactttat agcttccggt
     6121 ataagttcct tttcggaagt taaagttaag ttatcgaatc gagctaaagg tcatataact
     6181 atcctactct cagccttctt actactcaag gcctgggatt accgtttagc aatgtatgag
     6241 ttgttatact cctcccgggg agtggccttt ggagcgagtt atacagacat taatgctaat
     6301 ttattgggct taagaatact attcgtaatt gcgatagcgg ttgctcttgg tctgttagtt
     6361 agtctattta gaaaaaatta caaattatta gcctggggcc ttggaatctg gattgtaact
     6421 tctttaatat tcggtggtat ttatcctggt tttgttcaga gatttcaggt agaaccaaac
     6481 gaaattaata gagaacgtcc ctatataaat cacaatattc aaatgacctt agatgcttat
     6541 gatttaaatg atattcaaac cacagatttt gaggttgaag atggtttgac ctacgaagat
     6601 ttacaagaaa atgatgagat cgttaaaaat attagattat gggaccatag accattacta
     6661 accacctata atcagattca gactttaaga cagtactatg attttcaaaa tatagatgtt
     6721 gataggtata atataggtgg ggattatcgt caggtaatgc taggtgccag agagatgaac
     6781 caggacttat tatccgggca ggctcaaacc tggattaata aaaaattaaa gtatacacat
     6841 ggttatggtg ttacaatgag tcctgtaaat atggttacta gtgagggatt acctgagttt
     6901 ttcatgagtg atatacctac tcaagttaat gtagatattc ctttagaaaa tgctgcaatc
     6961 tattatggag aaaaaacgga tgaatatgta atcgctaata ctgattctac tgagtttcat
     7021 tacccacagg gagacgataa tgtttatata aattatgatg gaaccggtgg agtattagcg
     7081 ggcagtttta tcaaaaaagc tgtgtttgcc ctaaggttca atgaacttaa gtttttatta
     7141 aatgatgata taaatcctga cagtagagtt atgtatcaca gaaatattaa ggaaagagtg
     7201 agaagagttg ctcctttctt gcagtatgat ggggacccct acctggtagt ttctgatggt
     7261 agacttttct ggattcaaga tgcttacaca actacagata ggtatcctta ttctcaacct
     7321 gtgagagggg taggcaatta tattagaaat tcaattaaag ttgttataga tgcctataat
     7381 ggaactatga attattatgt tgtagatgat gaggatccga ttgcaaaaac ctatcaaaag
     7441 atattccctg atgtattttt agatggggaa gagatgccgg aagatataag agatcatatc
     7501 agatatccaa aagatctctt cagtattcaa acccaggttt atgcgactta ccatatgcag
     7561 gatccggttg tcttttataa taaggaagat ctctggaata ttcccactga aaattatgca
     7621 ggcagcagta tccagatgga accctattat ataacaagta agttaccggg taatgataaa
     7681 ttggaattta ttttaatgct tcctttcact cctgcaaaca gaaataatat gatttcctgg
     7741 atagcaggga gatctgatgg tgaaaattac ggggaactgt tagaatatca attcccaaaa
     7801 gatacccttg tcttcggacc taatcaaatt gaatcaagaa tagatcagga ttcagagata
     7861 tctcaaaggc tttccttatg gagtcaaagg ggatcaaatg taattagagg taatctgata
     7921 gtttatccaa ttgaaagatc tatattatat gttgaaccga tttatctaca ggctgaatcg
     7981 ggtgaaattc cagaacttaa aaggattgta gtaagttatg gtggagaaat agtaatggat
     8041 gaaaccttag atggtgcctt agcgaagata tttggagaaa aaccacaaga agaagttccc
     8101 gaagaggttc ctcaaagtca acagatgtta ccagaagata tgcagggact tgctcagaga
     8161 gcctatgaaa tattccaaga agcccaacag gctcaacaag agggtaactg gtcagaatat
     8221 ggtaacttat tagaagaatt aaataatgta ctaaatgaat taaatgatct cagtcaacaa
     8281 acagcagagt aattaggaaa ggataatgta tgcttaaaac aagtaaattc ggtgaatata
     8341 aaacagaaaa tgatatagac gtatatataa ataatactga taaatttaaa actaattata
     8401 ttcatataga tataatacga cctctttcta atgaaaaaga ggtctcccaa aatgcgctaa
     8461 tcccctatat tctttaccgc gggtctcaaa agtatcccac caatcaatct atatcacata
     8521 gattggatga tctatatggg gcagacttaa atgtatctgt ggcccgccgc ggtgagaacc
     8581 aactactaag gttttcggta gaagtaataa atgacaagta tgttgaagca aaagagagct
     8641 taataaaaaa ggcaattgat ttcctttatg aactgatttt tagaccttta gtagaaaagg
     8701 gggacttcaa ggaagaatat attaacaaag ggcagtcttt tttgaaggaa aaaatactat
     8761 ctttaaagaa tgataaaggt agttatgtta ttgaaagatg ttaccaggag atgtgtagag
     8821 gggaggattt ttccctctac acccttggta caattagtgg gttggaacaa agcaggcagg
     8881 ataatttgta tcaatattgg caagatatat taatagaaag tccaatcgaa ttatttgtca
     8941 tcggtgatat tggtgattct gatggactat ataaatatat caatcaaaag tttacagatc
     9001 acagagataa aaaattacct ttaaataaca cttttattaa aactaaagat tttaagttaa
     9061 aagagctgga agaagaggat gaaataactc aatctagatt ttgtgcgggt attagaatac
     9121 ctataaccag aagtcatccc ctctattttg ctcttattgt atataatggg atgttaggtg
     9181 gttttagcca ctctaagctc tttacaaccg ttagagaaaa gaggggctta gcctactata
     9241 tcaatacccg cttagaatct acaaagggcc ttatcttaat aaatgcaggc attgatatgg
     9301 tggattaccc gattgtaaaa gagataattg aagaaaaaat agaagagatg caaaatggtg
     9361 agtttgaaga ggaaaatatt gagtggtcta aaaagaaatt aattaatagg tataaaacgt
     9421 taagtgataa taaccacagt attgtggatg ctttcctatt aggtttaatc aatggtaaaa
     9481 aagattcatt aaattatgtt attaattcta tcaaacaggt aaaaagagaa gatataatta
     9541 aagttgctaa tcaggttaat cttacaacat attatctact tagaccagga agtgcttaag
     9601 aatgatatat aaaaatttta gtaatattgg tgaagaatac tatgaaaaga agttagattc
     9661 cggcttaaat gtggtattaa taccgcggga gaagtttaat aaaaaacatg cgatattatc
     9721 ggtagatgta ggttctgtag atttaaagat aaaagataat gaggaaaatg tctatgaata
     9781 tcccaaaggt attgcccatt ttatggaaca tatacttttt gaaaatagag aaggtagtat
     9841 aatagataag ttttcccaga ttggaagtag tgttaatgcc tatactggct ttaataatac
     9901 aaattattac tttaatacca ccagtaattt tttcaactcc ttaaaatact tatttggttt
     9961 tgttttaaat cctcattttg atgatgaacg gattaagcat gaaaaaagcg ttataaatca
    10021 ggaaatcaac atgtatcagg atagtcccag ctggcagtcc ttttatcaat tattacaatc
    10081 tcttttttac gaatatccaa ttaaatataa tattgcagga actcaaaaga gtatagagaa
    10141 aataaataaa aagacattaa ttgatttttt tgaaaaatta tataatttaa ataatatgac
    10201 cttaattgtc atcggggcgg ttcagcctga taagttattt aattggctgg aaaaagaaaa
    10261 aaatattact aaacaaggaa ataaaaaatt tgtattagag aaaatttatc ctaaagagcc
    10321 caatggaatt cgtctggaag aaaaagatat cgcaatgagt atctcccgcc cgataattag
    10381 tttaggttat aaagaaaacc gctgttttca aaataaagat aagattatta aacaggatct
    10441 tatgacaaat atgatcttag aaatgatttt tggtaaaagc agtgatcatt accatgcttt
    10501 atataatcaa gatttaattg atgagaactt ttcttttaac tatatgagag agaaaaatac
    10561 ggggtttgct aagatatatg cggaaaccaa ctccgttgat aagacaattt ctggtataac
    10621 cgatatcatc aataattggg aacgcgaaat aaatagcgaa gattttgaag tagttaaaca
    10681 taaatatatt ggaagatatt tccgttcttt aaattctttt gaaactcttt cttctcaaat
    10741 tatgaagtat cttaatcttg accaaaatta tttggatata attcaaatga tcaaagatat
    10801 aagtcttaaa gaagtaagag agcagtataa aaatttacta gaggaaaagg agcatgtgaa
    10861 aataatcatt agaacagttc ataagtaatt gacaaaataa tgcttgaaaa ctttagtttt
    10921 tggtgttata atcacaagaa aataataatg ggaaggggtg ataattaagg agatagtagt
    10981 agagcaactt ttattttcat tattattatt ttttaaagag aatattgttt aaaaagtgag
    11041 tatcaaaaag ttaagagaag aaaggagtag atagtagata tgcaaagaga ccgatggagt
    11101 aatcgtgcag cttttgtact cgcagctatt ggttctgcag ctggtttagg taatgcttgg
    11161 cgttttcctt acatggctta ttctaatggg ggtggagctt tcttaatccc ttactttgta
    11221 gctttattaa cagcaggggt accccttatc gtattagaat ttgccatggg gcaaaagacc
    11281 caaagaggtg ccccaggtgc cctagctaaa gttggtagaa aatttgaagg ctttggttgg
    11341 tgggccgttt tatccggtac tattattact agttattatg caggagttat ggcctgggct
    11401 tgggactatt tagctggttc ctttacacta ggttgggcag aaaatgcaag tgattatttt
    11461 tataatgaag tactgcaaat aacatctgga ccaggtgtct taggaggttt ttcagttccc
    11521 gttctgattg gtttagtcat cacctggatc gtaatttatc tcatacttag aaaaggtgct
    11581 gaaagtgttg gtaaagtggt tctaataaca gttcctttac ctgttctttt aatgattata
    11641 ttaatgctta gagcaataac tttaccaggc gctatggaag gaattaatta cttccttgaa
    11701 cctaatttct cgatgttgac gaatttaagt gtttggatcg atgcttattc tcaggtattc
    11761 tttacactta gtttaggttt tggtattatg attgcctatg ctagttatat gcctgaagat
    11821 tctgatatta ttaataatgc tcttataact gtatttgcaa actctggtat tagttttcta
    11881 gcaggtattg ctgtatttgg taccctaggc tatatggctc aaactcaggg agtaccgatt
    11941 tccgaagttg ctactgctgg tgttggatta gctttcgtgg tttatccaaa tgcgatatca
    12001 ctgttgccag gtggttcatt cgtggtcgga ttctttggtt tcgtcttctt cgcaatgctg
    12061 ctttccttag gtatagactc ggccttctcc attgtagaag ccggtgtagc agcacttaaa
    12121 gataaatggg gcggaaccaa aaggacagtt acactatttt atagtatatt cctatttgta
    12181 gtaggactat tatttgccac tagagctggt ctgtattggc ttgatattgt agaccattat
    12241 gtaaatactt atggcctcgt tgtagctggt ttagctgaat cattaattat tggttggttc
    12301 tatgacccca atgaattaag gggatatttc aactcagttt ctgaatatca gttcggcagc
    12361 tggtggaatg tgatgatcaa atatgtaaca ccgattatgt tagttgtact attagtcaat
    12421 gaatttattg ctgatattgg tactgcatat ggcggatatg aactaagata tctcatttac
    12481 ggcggctggg ctggagtaat tggtctatta cttgttgctt catttatcct atcacgtcaa
    12541 agaggtttag atgacgcaga aatctaaaaa ataagtattt agaagggagt aatattatga
    12601 g
//
LOCUS       DATGCJ010000117         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_639991, whole genome shotgun sequence.
ACCESSION   DATGCJ010000117 DATGCJ010000000
VERSION     DATGCJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639991"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..2340)
                     /gene="gyrB"
                     /locus_tag="VKN64_05845"
     CDS             complement(433..2340)
                     /gene="gyrB"
                     /locus_tag="VKN64_05845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMA59799.1"
                     /translation="MDLFDNDRYNADKIEVLEGLEAVRKRPGMYIGSTGIKGLHHLVW
                     EVVDNSIDEFLAGFGNKIKVTVHEDNSVSVEDWGRGIPADLHKDTGRPAVEIVLTTLH
                     AGGKFNNNSYKVAGGLHGVGVSVVNALSEELKIETHWEGHKYVQHYKRGLPQDNLAKD
                     GKTDKSGTKITFKPDREIFDTTKFKFETLSHRFKQSAYLNKDLTLVLEDERNDKKEQY
                     QYDGGIKAFIEYLNKNRDLLHDEIIYNEDSIDEYELEFAIQYNQGYSERIFSYANNIN
                     TVDGGYHLTGFKSALTKAVNNFAKKNNLLPKSVNGLNGHDVREGITAVINVKLPNPQF
                     EGQTKSKLGNSELRSIVEKSAYDYLSLYLDTNPELGKAIVNKALKSVRARQASKKARE
                     LTKRKSVLSSSSLPGKLADCASKKAEDSEIFIVEGDSAGGSAKQARNRHNQAILPLKG
                     KILNVERARLDKILNNTEIASIITALGTGVGEDFDLSNLRYEKIIIMTDADIDGAHIS
                     TLILTLFYRYMQDLIEEGHVYLARPPLYKVTYRKKSQYIFNEQGLEEYREEVGSGNFS
                     IQRYKGLGEMNPDQLWDTTMSPETRKLQKVEINDEIEADDVFTKLMGSNSKLRKKFIF
                     KNADLATEIDI"
     gene            2552..>2983
                     /locus_tag="VKN64_05850"
     CDS             2552..>2983
                     /locus_tag="VKN64_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMA59800.1"
                     /translation="MSEKISSIAIEEKMKEAYLNYSLSVIVSRALPDVRDGLKPVHRR
                     ILYAAEELGLTHDKDHKKSARIVGEVLGKYHPHGDQTVYNAMVRMAQNFNQRYTLIDG
                     HGNFGSIDGDSPAAMRYTEAKLTEMSEYLLKDIKKETVNFSD"
BASE COUNT          923 a          529 c          479 g         1052 t
ORIGIN      
        1 agaggttaag tcaaaataaa tttgtttgtg ataatcaaca tctacaaaat gctgattata
       61 atgcaagtat caatatagct aaaagagcta tccaatgata gcatacaggg taacctgtag
      121 cccctattgt ttttacagta gggatagggt ttttagtcgc tgcccttcaa ctttctctat
      181 tcttgaatca cccacatgtg agatagagaa ggtgtaaatc ttgttaaaga ctgtactgag
      241 tacggttttt aatataacaa gctccttttt aatcttattt ttgattaggt gagagaagtt
      301 gactggactc cctcttttct ttcttcttat ctttattata taatttttaa aggaaaaaat
      361 aaaaataact attaagaatg agaataagac aataaaagat cccacaattt aaaaaattgc
      421 gggattaaat ttctagatat ctatttcggt tgccaggtca gcgtttttga atataaattt
      481 ctttctcaat tttgagttgg aacccattaa ttttgtaaag acatcatcag cttcaatttc
      541 atcattaatc tctaccttct gaagtttacg ggtttcaggt gacatggtag tatcccaaag
      601 ttgatcagga ttcatttctc ctaaaccctt atatctttga atactgaaat taccggagcc
      661 aacttcttct ctatattctt caaggccttg ttcgttaaaa atatactggc ttttttttcg
      721 atatgtaact ttatataaag gtggacgggc taaatagaca tgtccttctt caataagatc
      781 ctgcatatat ctataaaata aggttaagat tagtgtagag atgtgagcac catcgatatc
      841 tgcatctgtc atgataatga tcttttcata tctgagatta cttaaatcaa agtcttcccc
      901 tacacctgtt cctaaggcag taatgataga agctatctca gtgttgttta aaatcttatc
      961 taagcgagcc ctttccacat taagtatttt acccttaagt ggtaaaatag cctgattatg
     1021 acggtttcta gcctgtttag cggagccccc ggctgaatcc ccctcaacga taaatatttc
     1081 gctgtcttct gcctttttac tggcacagtc ggctagttta ccaggaagag aacttgaaga
     1141 aagtactgac tttcttttag ttaattcacg ggcttttttg gaagcctgac gtgccctcac
     1201 agattttagt gctttattta caatagcttt acccaattca gggttagtat caagataaag
     1261 gctgagatag tcataagcgc ttttttctac aatgcttctc aattcactat tacccagttt
     1321 ggatttggtc tggccttcaa actgagggtt tggtaatttc acgttaatga ctgcggttat
     1381 accttcccgt acatcatgac catttaggcc gttaacgctt ttaggaagga gattattctt
     1441 ttttgcaaaa ttattaaccg ctttagttaa ggcagattta aaaccagtta gatggtaacc
     1501 tccatcaact gtattaatat tattagcata ggaaaaaatc cgttctgaat atccctgatt
     1561 gtattgaata gcaaattcta attcatattc atcaattgaa tcttcattat agatgatttc
     1621 atcatgaagt aagtctctat ttttatttag atactctata aatgctttta ttccaccatc
     1681 gtattgatat tgttcctttt tatcattacg ttcatcttct aaaacaagag ttaaatcttt
     1741 atttaaatag gcagactgtt taaatctatg ggataatgtt tcaaacttaa atttagttgt
     1801 atcaaaaatt tctcgatctg gtttaaaggt aatctttgta cctgatttat cggttttgcc
     1861 atctttagct aaattatctt gaggtagtcc ccttttatag tgctggacat atttatgacc
     1921 ttcccaatgg gtttcaatct ttaattcttc cgaaagagca tttactacgg aaactcctac
     1981 tccgtgtagt ccaccagcaa ctttataact gttattatta aacttaccac cagcatgtaa
     2041 ggtagtgagt acaatttcta cagccggacg tcctgtatct ttatgtagat cagcgggaat
     2101 acctcttccc cagtcttcta cacttacact gttatcttca tgtacagtaa ctttaatttt
     2161 attgccaaaa ccggctaaaa attcatcgat actattatcc actacttccc agacaagatg
     2221 gtgtagtcct ttaataccgg tagagccaat atacataccg ggtcttttcc gcactgcttc
     2281 cagaccctct aatacttcta ttttatctgc attatatcta tcattgtcaa ataagtccac
     2341 gaattcatcc accctttctt catattaacc attattatta taacaaagag tttatggatt
     2401 taccacccat tcttgcaata atgtggctag atggtataat atcaaatagg ataaagattt
     2461 taaagtacta atttttgctt aaatgttatt atatttattt atatattttt aatataattt
     2521 ttaaatatct aaattttgaa aggggtatat tatgtctgag aaaatttcat caattgcaat
     2581 tgaagaaaaa atgaaagaag cgtatcttaa ttattcactt agtgttatag tttcaagagc
     2641 cttacctgat gtaagagatg gtctaaaacc ggtacataga agaatcctct atgctgctga
     2701 agaactaggt ttgactcatg ataaggacca taagaaatca gctagaattg taggtgaagt
     2761 attaggtaaa taccatcctc atggtgacca aaccgtttat aatgctatgg taaggatggc
     2821 ccagaatttt aaccaaagat atactttaat cgatggacat ggtaactttg gttcaatcga
     2881 tggagactca cctgcagcaa tgcgttatac agaagctaaa ttaacagaga tgtctgaata
     2941 tcttttaaag gatataaaaa aggaaactgt aaatttcagt gat
//
LOCUS       DATGCJ010000118         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_104928, whole genome shotgun sequence.
ACCESSION   DATGCJ010000118 DATGCJ010000000
VERSION     DATGCJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104928"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1125
                     /locus_tag="VKN64_05855"
     CDS             97..1125
                     /locus_tag="VKN64_05855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMA59801.1"
                     /translation="MHFKKVLSIVMILTLLVAFPVAAQDNEGTSNTGEESSSQPEVAA
                     VVNGEDITMQELDQFAGTQNLVMTLYRQNPEFTQVLLQTEAGQNVITEFQKVKLDQLI
                     TQKLLAQEAKEREIALSEEEKTEIFNKQLEGIKQQNNIDDAQLEEALQQQGIESIDAY
                     RERFFQVNQESLLINKLQEDIQGQAEVTEEEMKEYYDNNKSNFEYQEQVKASHILLEE
                     QETAEEVMTKLDEGGDFAELAKEYSTGPSAEKGGDLGYISKDQNIAQKFKEAVFALDS
                     GEMSQIVETQYGFHIIKATDRREAGVRGFEEVKDQIKQQLQNQKGQQIWDEFVRKLRD
                     EAEIEKKL"
     gene            1321..2376
                     /locus_tag="VKN64_05860"
     CDS             1321..2376
                     /locus_tag="VKN64_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMA59802.1"
                     /translation="MENTKSKLEELNIKRYLIYLFIFIVISVLTLYYIYRIFGQETSF
                     LIFKDFPAPIIIGLFSLLLIYFVLDGFRLYFILRTLEIKISYWHIFKIVFINLFISNI
                     TPFASGGGVAQVYFLNKKGVPLGNATAATTIRTVLASVFVFVSGPLILLTRERIFNQF
                     SGVPFFGYLLAFFGLYLFIFYVAIFRNRLIKKTVFVTMRFLKGKKLLSKKKYNSTLKY
                     LLKHIEIFGEKLALFLKGKTRYVVLSFFFTALFLLSEFTFAILLLEGMGYQFNYLTII
                     LMQMVVIFFMYFAPTPGATGVAEGGFSLLFTRIVEKGDILPLIFSWRLFTKYIGIAIG
                     IIVFYLMLFKGAKPDEE"
     gene            2366..>2875
                     /locus_tag="VKN64_05865"
     CDS             2366..>2875
                     /locus_tag="VKN64_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59803.1"
                     /translation="MKNKTKVFFIILIAAIIILFTYKIYAPFSIQDFNAVNYKSISQV
                     KREVKDNSFSFAVLGNIKNSISIFDKGILPRLRSDRPDFIISTGNNVVDSGEGKYRVL
                     YRTLQRMKIPFITSVGENEIEEEGHKNFYKYFGPFYFSFNLDDSYFIFLDTTGHSSES
                     WQREWLNKEL"
BASE COUNT          997 a          436 c          548 g          894 t
ORIGIN      
        1 tcgagtcctg ctcggggagc catttttttg accgcatgca ataaaaaact aaataatatt
       61 tattaattaa actcatgagg agagtgaatt aaatttatgc atttcaaaaa agtattatca
      121 atagtgatga tattaaccct actagttgca tttccagttg cagcacagga taatgaagga
      181 acatccaata caggtgaaga aagcagcagc cagcccgaag tagctgccgt agttaatggt
      241 gaagacatca ccatgcagga gcttgatcaa tttgctggca cccaaaatct cgtaatgact
      301 ttatatagac agaatcctga attcacccag gtacttttac agactgaagc tggacaaaat
      361 gtaattactg aattccaaaa agttaaactt gaccaattga tcactcaaaa actactagcc
      421 caagaagcaa aagaaagaga gattgcttta tcagaagaag aaaaaacaga gatctttaat
      481 aaacagcttg aaggaatcaa acagcagaat aatatagatg atgcccagtt agaagaagct
      541 ttacaacaac agggtattga atctatagat gcttatagag aaagattctt ccaggtaaat
      601 caagagagtc ttctaatcaa taaattacag gaagatattc aaggtcaggc agaagttacc
      661 gaagaagaaa tgaaagagta ctatgataat aataaatcta attttgagta tcaggaacag
      721 gtaaaggcaa gccatattct acttgaagaa caggaaactg ccgaagaagt tatgactaaa
      781 ttagatgaag gcggagactt tgctgaatta gccaaagaat attcaaccgg tccctctgcg
      841 gaaaaaggtg gagacctagg ctatatcagt aaggatcaaa atattgctca gaaatttaaa
      901 gaagcagtat ttgccctaga ctccggtgaa atgagtcaga tagtagaaac ccagtacggc
      961 ttccatatta tcaaagcaac tgatagaaga gaagctggcg taagaggatt tgaagaagta
     1021 aaagatcaga tcaaacaaca attacaaaat caaaaaggac aacaaatctg ggatgaattt
     1081 gtaagaaaac tcagagatga agctgaaata gaaaagaaac tataaattaa gataaagtaa
     1141 gttcaataat aaaaagacta ggaggtgtct gtaagggcgc ctcctttttt taatgtttaa
     1201 aatgttaaat tttgcaggtt taaaagagat atatgtagaa actaattaat agaggttaag
     1261 aaataaaata gtgaaataga ttagatattc ttttaggtaa cctaggagct ggaggtaatt
     1321 atggaaaata caaaaagcaa attagaagaa ttaaatataa aaagatacct tatctacctg
     1381 tttatcttta ttgtaatatc agttttaacc ttatattaca tatatagaat atttggccag
     1441 gaaaccagtt ttttaatttt taaagatttt ccggccccca tcattatagg attattttct
     1501 ttactattaa tatattttgt tctggatggt tttaggcttt actttattct tagaacctta
     1561 gagataaaga ttagttattg gcatattttt aagatcgtct ttattaatct ttttatttcc
     1621 aatattactc cctttgccag cgggggaggg gtagcacagg tttatttttt aaataagaag
     1681 ggtgttccct tgggtaatgc gacagctgct accacaatca gaacagtatt agcctctgta
     1741 tttgtttttg taagtggccc tttaatactt ttaaccagag aaaggatatt caaccagttt
     1801 tcgggtgtcc ccttctttgg ttatctatta gcattttttg gactctatct ttttattttt
     1861 tatgtagcta tatttagaaa tcggttgata aagaaaacag tatttgtaac gatgcgcttt
     1921 ttgaaaggga aaaagctgct ttcaaaaaag aaatataata gtactttgaa gtacttacta
     1981 aagcatatag agatttttgg agaaaaatta gcattatttt taaaagggaa gactagatat
     2041 gtagtactct cctttttctt taccgccctg tttttattgt ctgaattcac ctttgcgata
     2101 cttttattag aaggtatggg ttatcagttt aattatctaa ccataatttt aatgcagatg
     2161 gtagtcatat tttttatgta ctttgcccct actcctggtg ctaccggggt agctgaaggc
     2221 ggtttttccc tgctgtttac cagaattgtg gagaagggag atatcttacc cctgattttt
     2281 tcctggcggc tgtttaccaa gtatatcggg attgcaattg gaataatagt attttacctt
     2341 atgttattta aaggggccaa accagatgaa gaataaaact aaggtgtttt tcataatctt
     2401 aatagcggct ataataattc tttttaccta caagatctat gccccgtttt ctattcagga
     2461 ttttaacgct gttaattata aaagtattag ccaggtaaaa agggaagtaa aagataattc
     2521 tttttccttt gcagtactgg gcaatatcaa aaattcgatc agcatctttg ataaaggcat
     2581 tttacccaga ttacgatctg atagaccgga tttcatcatt tccaccggta ataatgtggt
     2641 tgacagtgga gaaggtaaat atagagtttt atatagaacc ttgcagagaa tgaagatacc
     2701 ttttattacc agtgtgggag aaaatgagat cgaggaggaa gggcataaga acttctacaa
     2761 atacttcgga cccttctatt tttcttttaa tctcgatgat tcctacttta tctttctcga
     2821 tacgaccggc cacagttctg aaagctggca gcgggaatgg cttaataagg aatta
//
LOCUS       DATGCJ010000119         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_506263, whole genome shotgun sequence.
ACCESSION   DATGCJ010000119 DATGCJ010000000
VERSION     DATGCJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506263"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..1872
                     /locus_tag="VKN64_05870"
     CDS             589..1872
                     /locus_tag="VKN64_05870"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMA59804.1"
                     /translation="MIKIGLLGLGTVGSGVYKIISENRESIEQKVGTELEIEKILVND
                     LSKTRNVKIDNELLTDNADDILENTDINLVVELIGGEEPAYQYVMQAVNNKKSVITAN
                     KLIIAKHGSEILKAASQNDVQISFEGSVAGGIPIIRPLKESLVASNIHNIYGILNGTT
                     NYILTKMTEEEKEYQDVLKEAQELGYAESDPYSDVSGLDAAYKIAILSSITFETYVDI
                     NSVYSEGIEDITLTDIQVADELGYVIKLLAIGKKQEDGIDIRVHPAFISKNHPLALVN
                     GVYNAIYLHGEIVGDIMSYGKGAGQMPTGSAVVADIIQTAKDIHYNRPNVYADKSTLK
                     HKIVNIDEIKNLFYLRVLVNDKPGVMSEVTGILGKNKVSLASVLQKHRLTPVVPLDLV
                     THPVKEKNLNNSLAELKSLKNVKSIESVIRVIEDF"
     gene            complement(1962..2201)
                     /locus_tag="VKN64_05875"
     CDS             complement(1962..2201)
                     /locus_tag="VKN64_05875"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA59805.1"
                     /translation="MSKNRRNRDRKRRFKRSSNQTNKIINIDQEKCIGCGQCVSACPF
                     NAIETNEEGAAYVIPNKCRGCKQCIRMCPVQAISI"
     gene            complement(2338..>2694)
                     /locus_tag="VKN64_05880"
     CDS             complement(2338..>2694)
                     /locus_tag="VKN64_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59806.1"
                     /translation="GLYGGMNQQSVEPHRWEMEPFNGGWSSSLFISQDPIAIDSVALD
                     FLKTETDLLPYSDNYLHEGALANDPPSGVKYDPENNGTRLDSLGVHEHWNNDKEKLYS
                     KNINPESKGIELIKMK"
BASE COUNT          916 a          452 c          423 g          903 t
ORIGIN      
        1 cacccttttg tacatcctgg gctaaagttc ctactgtaaa taacattact gataagattg
       61 aaaacaataa aatcgctgta atttttttct tcatttaata tcctcctatt tatgaatttc
      121 ctgaatttaa aatttatcta ctcccctgct ttttaatact cacttcagaa aatttttcta
      181 ccaccacctt ttttaaaatt attatttttt aattatgagt aatgttgtca agtataatta
      241 ttaatctttt gattatactt attcaacatt acaatgtata atcctcccta atagataata
      301 atataaacta gttagttaat ataacagcgc ttaccaaatt atacttgtca tatgacataa
      361 atcttcatta tatatttatg caaaaagtat accaaagttg ctttcctatt ttcacaataa
      421 tcacacaatt tttctatata ataaatggga ttatatatat tctcgattaa tataatccca
      481 tttctattat tatgagacta taatcatata attgaaatta ttattggaca tatgcctatt
      541 aaaagtgtaa aatatatgaa aagaagtatt gagaagggag aattagatat gattaaaatt
      601 ggcctcctcg gattaggaac agtcggctcc ggggtatata aaataataag tgaaaataga
      661 gaaagtattg agcaaaaagt aggcactgaa ttagaaattg aaaaaatact tgttaatgat
      721 ctcagtaaaa caagaaatgt taaaatcgat aatgaacttt taacagataa tgctgatgat
      781 attttagaaa ataccgatat taatctggtt gtggaattaa taggtggaga agaacccgct
      841 tatcaatatg ttatgcaggc tgtgaataat aaaaaaagcg taattaccgc caataagtta
      901 attattgcca aacacggaag tgaaattctc aaggcagcca gtcaaaatga tgtgcagatc
      961 agttttgaag gtagtgtagc aggcgggata ccgatcatcc gtcccttaaa agaatctctt
     1021 gttgccagta atatccataa tatatacggg atattaaacg gcactaccaa ctatatatta
     1081 actaaaatga cagaagaaga aaaagaatac caggatgttt taaaagaagc tcaagaatta
     1141 ggttatgcgg aaagtgaccc ctattctgat gtcagcggac ttgatgctgc ctataaaata
     1201 gccatcttat cttctattac ttttgaaaca tatgttgata ttaactctgt ttattcagaa
     1261 gggatcgaag atattacttt aactgatatt caggtggctg atgaattggg ttatgttatt
     1321 aaactattag ctatcggcaa aaaacaggaa gatggaattg atattagggt ccatcccgcc
     1381 tttatctcaa aaaatcaccc acttgcttta gttaatggtg tatataatgc aatttatctg
     1441 catggtgaaa ttgtagggga tataatgtct tatggaaaag gggccggtca aatgccgacc
     1501 ggaagtgctg tggtggcaga tattattcaa actgccaaag acatccatta taatagacct
     1561 aatgtttatg cggataaatc aaccttaaaa cataagattg ttaatatcga tgaaataaaa
     1621 aacctgtttt atttaagggt attagttaat gataaacccg gtgtaatgtc tgaagtaacc
     1681 ggtattttag gtaaaaacaa ggttagtttg gcctctgttc tgcaaaaaca tcgcctaaca
     1741 cccgttgtac cattagattt agtcacacat cccgtcaaag aaaaaaatct gaataactcc
     1801 cttgcagaat tgaaaagctt aaaaaatgta aaatcaattg aaagtgtaat aagggttatt
     1861 gaagactttt aatatatatt tttaaaagcc ccatttaagg ggcttttttt aattaaagta
     1921 taagtatctc atttctctct ataaatttta aattttttta tctatattga gatagcctgc
     1981 actggacaca ttctaataca ttgtttacat cccctgcact tatttgggat cacataggct
     2041 gctccttcct cattcgtttc gatagcatta aaaggacagg ctgatacaca ctgaccacaa
     2101 cctatacact tttcttggtc aatatttata attttattgg tctgattact gcttctttta
     2161 aaccttctct ttcggtctct atttcttctg tttttggaca cggctacccc tccttcattt
     2221 aactgtgaaa attatataat agttatgaac atatgtcaaa tattgccggt gaaattttac
     2281 ccataaaaaa actcataggc ttttgcttag cctatgagct taaaagttaa atattattta
     2341 tttcatcttt atcaattcaa ttcctttgct ctcgggatta atatttttac tatacagttt
     2401 ctctttatca ttattccagt gttcatgcac acccaaacta tctagccgag tcccattgtt
     2461 ctctgggtca tatttaactc cagaaggagg atcattggcc agagctcctt catgtaagta
     2521 gttatctgag taaggtaata aatcagtctc ggtctttaaa aaatccaggg ctaccgagtc
     2581 aattgcaatc gggtcctgtg aaataaataa actagaagac cagccgccat tgaatggttc
     2641 catctcccat ctatggggct caactgactg ctgattcata ccaccatata aacc
//
LOCUS       DATGCJ010000120         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_506271, whole genome shotgun sequence.
ACCESSION   DATGCJ010000120 DATGCJ010000000
VERSION     DATGCJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506271"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1370
                     /locus_tag="VKN64_05885"
     CDS             240..1370
                     /locus_tag="VKN64_05885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMA59807.1"
                     /translation="MFDYISSYLYPIARENKEDFKLHKFKQNNKRILLISAFLIFEQL
                     FYAVFISKSGTILNTVYYGTALIMFIYTSISAYFYFKQPKRVSFIHKLYQVGIGVIGL
                     TVAVIRIGILPNYIVRLPTIYIAVVYGMAVIFYFNYIESFFLYLYGALLLVYIASTYQ
                     PILLESNLVVDALSNIIIAWLASMIIFKKFVNEFINQKKIEDKNEKLNKVNAKLKEIS
                     DKDRLTGIYNRRKLEEILSDVYYKAERYNKNFSLILFDLDHFKKVNDNYGHQVGDKVL
                     KEISKILLNSIRKVDYFGRWGGEEFLIILPETNLQAAEKLAERLRGNIEQSSFSKVDN
                     LTSSFGVASYSEEKGIEDIIQEADQALYRAKEKGRNRVEKIA"
     gene            1398..2495
                     /locus_tag="VKN64_05890"
     CDS             1398..2495
                     /locus_tag="VKN64_05890"
                     /inference="COORDINATES: protein motif:HMM:NF010321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HMA59808.1"
                     /translation="MPSLNLMIKPVSSNCNMKCKYCFYHDLASNRERASYGKMDEKLL
                     ELIVKKAFTEAEGSVSFSFQGGEPTLAGLDFYKTFMDYLKKYNKDNIDVNLALQTNGI
                     KIDQKWAAFLSENDWLVGLSIDGYKDLHNYFRIDRIEEGTYKQVIKTSELFKKCNVDF
                     NVLTVITDQLARHGKKVYNSYKKHNFKFLQFIRCLEPLDGKKEKYSLTPENYELFLNN
                     IFNEWYQDIKKGNYISIRFFDNVVRMLMGRKPEACEMKGHCSFQNIIESDGSIYPCDF
                     YVVDEWKLGNIKNMSFTEVYESKKAKAFIKESTDLPKKCEQCKWLKLCRNGCKRNRDE
                     NNLNNYCTSFYNFYEKNINKLLELKRIFLNN"
     gene            2751..3541
                     /locus_tag="VKN64_05895"
                     /pseudo
     CDS             2751..3541
                     /locus_tag="VKN64_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(3610..4041)
                     /locus_tag="VKN64_05900"
     CDS             complement(3610..4041)
                     /locus_tag="VKN64_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA59809.1"
                     /translation="MFNGVLFSILSGILVSLQGIFNTRASEHIGLWHTNTLVHGSGFI
                     LALIILIAVNKVTFANIKNVNPYYLLGGVLGVLIVFSVMKGISALGASYAITILIVTQ
                     ILANAIINYFGIFGEHVITFSWTQIIGLLMMIGGIFLYQLT"
BASE COUNT         1563 a          558 c          779 g         1352 t
ORIGIN      
        1 acagatatta cttccggtct ccaatgtgga attaactacc tgcaaagagg gtattaaaaa
       61 aatataagat gatagttata acccccctta tgaaattaag gggggttaaa tttatgtctt
      121 ataattcaaa aagttttgaa atattgatca tatttttata taatgaaaaa caagtttatt
      181 tttaagaaca ttttttaaaa atcaagaatc cgagtgttat tttgattagg gggattttta
      241 tgttcgatta tatcagcagc tatctatatc caatagcgag agaaaataaa gaggacttta
      301 aattacataa atttaaacaa aataataaga ggattttatt aatatcagct tttttaatat
      361 ttgaacagtt attttatgct gtttttataa gtaaaagtgg taccatttta aatacggtct
      421 attatggaac tgctttaatt atgttcatat atacctcgat aagtgcctac ttctatttta
      481 aacaacccaa acgagtttct tttatacata aactctatca agtcgggatt ggtgtgatag
      541 gtctaactgt agcagtaatt agaataggta ttttgccgaa ttatattgtt agattaccaa
      601 caatttatat agcggtggtc tatggtatgg cggtcatatt ttattttaac tatatagaaa
      661 gctttttctt atacctatat ggagctctat tattagtcta tattgcttca acatatcagc
      721 cgatattatt agaaagtaat ctagttgttg atgcgttgtc caatatcata attgcctggt
      781 tagcttcgat gatcatcttt aaaaaatttg ttaatgaatt tattaatcaa aagaagattg
      841 aagataaaaa tgaaaaattg aataaggtca atgccaaatt aaaggaaatt tctgataagg
      901 atagattaac tggtatttat aaccgaagaa aattagaaga aatattatca gatgtttatt
      961 acaaagccga aagatataat aagaactttt ctttaattct atttgattta gatcatttta
     1021 aaaaagtaaa tgataactat gggcatcaag taggagataa ggtattaaaa gaaatcagta
     1081 aaattctttt aaatagcata aggaaggttg attattttgg ccgctgggga ggagaagaat
     1141 ttctaattat tctccccgag actaacttgc aggcggccga aaaattagct gaaagattaa
     1201 ggggtaatat agaacaaagt tctttttcca aagtggataa cctgactagt agttttggag
     1261 tggcttccta ttcagaagaa aaaggaattg aagatataat tcaagaagca gatcaggctt
     1321 tatatagagc taaagaaaaa ggccgtaacc gggtagaaaa aatagcctga agaaaaatat
     1381 tttggaggtt taaattcttg ccatctttaa atttaatgat caaaccggtt tcaagtaact
     1441 gcaacatgaa atgtaaatac tgtttttatc atgatttagc ttccaatcga gaaagagctt
     1501 cttatggaaa gatggatgaa aaattattgg aactaattgt aaaaaaagcg ttcactgaag
     1561 ccgagggatc agtcagtttt tcttttcagg gaggagaacc aactttagct gggttagatt
     1621 tttataaaac tttcatggat tatctgaaaa aatataataa agataatata gatgttaacc
     1681 ttgccctaca aaccaatggg ataaaaatag atcaaaagtg ggcagccttt ttatctgaga
     1741 atgattggtt agtaggctta tcgattgatg gttataaaga cttacataat tattttagaa
     1801 ttgatagaat tgaagaggga acctataaac aagtaataaa aacctctgaa ctttttaaaa
     1861 agtgtaatgt cgattttaat gtactgaccg ttataactga ccaactggct cgtcatggta
     1921 aaaaagtcta taatagttat aaaaagcata attttaagtt tttacagttt atacgctgtc
     1981 tagaacccct ggatgggaaa aaagaaaaat attcacttac ccctgagaat tatgagttgt
     2041 ttttaaataa tatatttaat gagtggtatc aggatatcaa aaaggggaat tatattagta
     2101 taagattttt tgacaatgtt gtgagaatgt tgatgggaag aaaaccggaa gcctgtgaaa
     2161 tgaaaggaca ctgcagtttc caaaatataa ttgaatcaga tggcagtatc tacccctgtg
     2221 atttctatgt agtagatgaa tggaagttag gaaatattaa aaacatgagt tttacggaag
     2281 tatatgaaag taaaaaagct aaagccttta ttaaagaatc gactgatttg ccgaaaaaat
     2341 gtgaacagtg taaatggctt aagttgtgta gaaatggttg taagaggaat agagatgaaa
     2401 ataatctcaa taattactgc acttcttttt ataattttta tgaaaaaaat ataaataaat
     2461 tattagaatt aaagagaata tttttgaata attaagtacc tggctttaaa aagagtcagg
     2521 tttttatttt atttcaaaat aaaatcatat ataatagatt taattaggag atgttaagga
     2581 ggataaaaat gaagatagtg atgttagaat caattggaat atcagaaaat gtacataagg
     2641 aatataaaga gaaattagag aatttaggcc atgaatttga atattatgat aatagggtag
     2701 aataagatag cgagataatt aaaagagttc aagatgcaga aataataatt attacaaact
     2761 taccattatc agaaaaagtc ataaattcat gtccgaacct gaagtttatc tcggttgcct
     2821 ttctaggagt agagcataat gctcttgaag cttgtaatga aaatgatatt gtagttagta
     2881 attcttccgg ttattctgac caatctgtgg ctgaattagt ttttgggttg acgatttctc
     2941 tcatgagaaa tgtcatgaaa ggagataggg caaccagaaa acagaaaacg agagctggaa
     3001 tcataggtaa tgaattagct aacaagaaat ttggtattgt aggtacaggt aacataggaa
     3061 cccaggtggc tacaatagtt gatgcctttg gttgtgagtt attaggaaat gatattcaag
     3121 aaaatgtaaa ggtaaaaaaa ttaggtttgc aatatgtggg cctggcggaa ctaatgaaga
     3181 aaagtgatat agtttcggtt catgtacccc ttatggaatc cacgctaaat ttaattggtg
     3241 aaaaagaaat aaatttaatg aaaaaactgc ctatctaatc aattgtgcgc ggggtccgat
     3301 agttgatagt gaggagttag cagagtcttt aaataatgat aaaatagcgg gagccggtat
     3361 tgatgtattt gagatggagc ctcccattcc caaagatcac ccacttttaa acgctaaaaa
     3421 cactatttta actccccatg tagcttttgc taccgatgag gcttttataa aaagggccga
     3481 gatagttttt aataatatta ccaaatggtt agaaaatgat cctcaaaata ttgtaaatta
     3541 aaaagccaat tattgataaa gcccctcact gaattcagga ggggctttct aagtgttatc
     3601 ttttatctat tatgttagtt gataaaggaa gataccaccg atcatcatca gtagaccgat
     3661 aatttgagtc caggaaaatg ttatcacatg ctctccaaag atgccaaagt aattaattat
     3721 agcattggca aggatttggg tcactattaa gatggtgata gcataactag cccctagagc
     3781 cgaaattcct ttcatcacac taaaaacaat taagaccccc aaaactccgc ctaataagta
     3841 ataagggttt acatttttaa tattagcaaa ggtaacttta tttaccgcaa tgagtataat
     3901 taaagccaga ataaacccgg atccgtgtac taaagtgttg gtatgccaga ggcctatgtg
     3961 ctcactggca cgggtattaa aaattccctg taatgagact aaaattccgg aaagtatgga
     4021 aaacaaaact ccattaaaca tttttttatc ctctcccatt caaattattt aaaccagatt
     4081 tttcaggctt aatatactaa attaccataa tgctatatga ccatcggttc ttttttcagt
     4141 accacctttt aaaacaccag tttcatggtc tctaaagata atctgtcctc tgccaaaact
     4201 gccagaatct aaggataaac taacttcatg tcctttgcgt tggagtgcct ga
//
LOCUS       DATGCJ010000121         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_281829, whole genome shotgun sequence.
ACCESSION   DATGCJ010000121 DATGCJ010000000
VERSION     DATGCJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281829"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..610)
                     /locus_tag="VKN64_05905"
     CDS             complement(113..610)
                     /locus_tag="VKN64_05905"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HMA59810.1"
                     /translation="MQSKNVLITGATGNVGHALLKKLTNEDNIKIFAAGRHVEKTKDV
                     IDVDNISYRCPDFKDKNTYKSSLQDIDTAFLMRPPAISKVKKYIKPYLVKIKEKNIKQ
                     VVFLSLQGADKNILVPHHRIEKYIKELEIPYTFLRPSFFMQNLSTTHKEEIKARSQLL
                     PPNQK"
     gene            complement(627..998)
                     /locus_tag="VKN64_05910"
     CDS             complement(627..998)
                     /locus_tag="VKN64_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA59811.1"
                     /translation="MINLTHCCIITDDVNELTNFYERILKIEADRYGENYSEIEAGDT
                     QLAIFDVFEQEGLAPGTARVRSNESLILEFNVEDLEAEYKRIKGMGIEMAKDITTQPW
                     GNTSFYFRDPDGNLINFYTQS"
     gene            complement(998..1798)
                     /locus_tag="VKN64_05915"
     CDS             complement(998..1798)
                     /locus_tag="VKN64_05915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA59812.1"
                     /translation="MIRKLNKDDQGSLMEYISEEPALNLFIIGDIENYGFQEDFQDIW
                     GDFNKKDEYRAVLLRYYDSFIIYAKSDYDVKRIANIIKDHKAKMVSGKKECVEELDNY
                     LNISQKRDTYFCKLDKVGQLYQGPLLEKVEKTQLEDVKLLWELHNRIDEFDGLDELDR
                     KIKKYKDKTGRGYHIYDEKREKIISCAETTAENSMSAMIVGVATDQDYRNQRLASAVM
                     SKLCEEVLNEGKELCLFYDNPSAGKIYKRIGFTDIGIWSMWSLNKEED"
     gene            complement(1820..>2228)
                     /locus_tag="VKN64_05920"
     CDS             complement(1820..>2228)
                     /locus_tag="VKN64_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMA59813.1"
                     /translation="EEYNIKLKEHDNIICGKSLGGLTAFYTAYDSELFNSIISHSGTF
                     IWGYPDARERDFLFEEIDNSNFSLKNIYLDVGTLEDEFVPMWFMSLIDVNKRMKNSIE
                     DKCERLFFNIFNGGHDYYCWRKNTVKALLKFYN"
BASE COUNT          637 a          457 c          315 g          819 t
ORIGIN      
        1 cccttcaact ttctctattc ttgaatcacc catatgtgag atagagaagg tgtaaatctt
       61 gttaaagact gtactgagta cggtttttaa tttaacaagc tccttcctaa tcttattttt
      121 gattaggtgg gagaagttga cttcttgctt taatctcttc tttgtgagtt gtagaaagat
      181 tctgcataaa aaaactcggc cttaagaatg tataaggaat ctctaactct tttatatatt
      241 tttctattct gtgatgggga accaaaatat tcttatccgc cccctgtaaa gatagaaata
      301 ctacctgttt aatatttttc tccttgattt taactaaata aggtttgata tactttttaa
      361 cctttgaaat agccggtggt ctcattaaaa aggcagtatc aatgtcttgt agggagcttt
      421 tatatgtatt cttatcctta aaatcaggac atctataact tatattatca acatcgatca
      481 catcttttgt tttttctaca tggcggccgg ctgcaaatat ttttatatta tcttcatttg
      541 ttaatttttt aagtaaagca tgccctacat tcccggttgc tccggtaatt aatacatttt
      601 tcgactgcat aagatgactc ctttatctag ctttgcgtat aaaagtttat caagttccca
      661 tctggatctc taaaataaaa ggacgtgttg ccccagggtt gagtagtgat atccttggcc
      721 atttctatcc ccataccctt tatcctcttg tattcggctt ccagatcctc aacattaaat
      781 tctaagatga gactttcatt tgaccttacc cgggcagttc ccggtgcaag accttcctgt
      841 tcaaaaacat caaaaattgc cagttgggta tcacctgctt cgatttcact atagttttct
      901 ccatatcgat ctgcttcaat ctttaatatc ctctcataaa agttggtcaa ttcatttaca
      961 tcatcagtta tgatgcagca gtgagttaag ttaatcatta atcttcctcc ttatttaaac
     1021 tccacatact ccagatcccg atatctgtaa agccgatccg cttatatatc tttccggcac
     1081 tgggattatc gtagaaaagg caaagttctt taccctcgtt taatacttct tcacacagtt
     1141 ttgacattac tgccgaagct aaacgctgat tgcggtaatc ctgatcggtc gcaactccaa
     1201 ctatcatagc tgacatgcta ttttctgctg tagtttcggc acagctgata atcttttctc
     1261 tcttttcatc atagatatgg tagcctcggc ctgttttatc cttatacttt tttatctttc
     1321 tatctaattc atctaagcca tcaaactcat cgattctatt atgtagttcc caaaggagtt
     1381 ttacatcttc taattgtgtc ttttctacct tctccaataa gggaccttga tagagttggc
     1441 ccactttatc aagtttacaa aaataggtgt ctcgcttttg agagatatta agataattat
     1501 ctaattcttc tacacattct ttcttgccgc tgaccatttt tgctttatga tctttgataa
     1561 tattagcaat tcttttcaca tcataatcag atttagcgta aatgataaaa ctatcataat
     1621 atctcaaaag aactgccctg tattcatctt ttttattgaa atcaccccag atatcctgaa
     1681 aatcttcttg aaatccatag ttttcaatat ctcctataat aaaaaggtta agggccggtt
     1741 cttcgcttat atattccatt aaactccctt gatcgtcttt atttagtttt cgaatcatga
     1801 acttaacctc cagtctgaat taattataga atttaagtaa ggcttttaca gtatttttac
     1861 gccagcagta ataatcatgt ccaccgttaa agatattaaa aaataatctt tcgcatttat
     1921 cttcaataga atttttcatc cttttattca catcaatcaa actcatgaac cacataggta
     1981 caaattcatc ttctaaagta cctacatcta gataaatatt ctttaaacta aaattggagt
     2041 tatctatctc ttcaaataga aagtcccttt cccttgcatc gggataaccc cagataaagg
     2101 taccggaatg actgataata ctattaaata gctctgaatc ataggcagta taaaaggcgg
     2161 ttaaaccccc caggctcttg ccacagataa tgttatcgtg ttcttttagc ttgatattgt
     2221 attcttct
//
LOCUS       DATGCJ010000122         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_461998, whole genome shotgun sequence.
ACCESSION   DATGCJ010000122 DATGCJ010000000
VERSION     DATGCJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461998"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VKN64_05925"
     CDS             complement(<1..87)
                     /locus_tag="VKN64_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA59814.1"
                     /translation="MKAVITGGGTGGHIYPALSVADKLSENNW"
     gene            complement(98..1192)
                     /gene="spoVE"
                     /locus_tag="VKN64_05930"
     CDS             complement(98..1192)
                     /gene="spoVE"
                     /locus_tag="VKN64_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HMA59815.1"
                     /translation="MNKKSADFILLFAVLTLLAIGIIMIFSASSIRGEDMFNDSYYFL
                     KRQAVFAVIGLIAMAIVMNIDYHIFEKYAKYIILFAIILLALVLIPGIGKKVGGSRRW
                     LGIAGLGGQPSEVAKLALIMYIAQFYTRKNNLIKSFKKGILPILIVTGIIFMLILIEP
                     DLGTASLILAIVFVMLFANGAKIWHLIGLGMVSVPAVFYFIYSEPYRKERFLAFLDPW
                     SDPLDTGYHIIQSLLALGSGGLFGVGLGNSHQKFLYLPEPGTDFIFAIIGEELGFLAT
                     LLVILLYLVVAWKGLKISYNTKDRFGSILAMGITVMIIVQAILNIAVVTASMPITGIT
                     LPFISYGGSSLVMFLFSVGILLNISKNQES"
     gene            complement(1215..2585)
                     /gene="murD"
                     /locus_tag="VKN64_05935"
     CDS             complement(1215..2585)
                     /gene="murD"
                     /locus_tag="VKN64_05935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMA59816.1"
                     /translation="MENFINKTVTIIGLSERTGLAVAEKMIDLGADVIISDIKSKDDL
                     KPQLSILSKYDNITYDFGGHSEEILDTDLIVLSPGVPTDLDIIKKAVQRNIEVISEIE
                     LAYRLTDANIIGITGTNGKTTVTSLTGHILKQNLDCKVRVAGNIGSPLVTEITGLSEN
                     DWLVVELSSFQLEKIKKFKPHIGVFLNFSPDHLDRHNTVENYWNAKKRIFENQNSDDI
                     AVINYDDNYLRDNLDDIKSNTFKVTNNFDIEQGIFYNKKSIYQIQKSVAGKSKILETK
                     DIPLIGSHNILNTAFAVLIAKLFYIDNEKIKKAVQSYQAFAHRLEVIYDQEYKVIDDS
                     KATNPHATINAMRSIEMPIILIAGGQDRKANFADFAYAVKQRVKKVILLGETKKEIKG
                     ELDNLNYNNYKLVDNMQEAVQEAFNEIKTYDTVLLSPGCPSWDMYESYKKRGLDFQQE
                     AQKFLK"
     gene            complement(2598..3563)
                     /gene="mraY"
                     /locus_tag="VKN64_05940"
     CDS             complement(2598..3563)
                     /gene="mraY"
                     /locus_tag="VKN64_05940"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMA59817.1"
                     /translation="MSYLMKSLIFFAAPLLWVLLLTPYFIKLLKKYELGQNVREVGPE
                     SHYEKEGIPTMGGLLIVTGIIIPVILTGSFSINNIIFILILVSTAIIGFSDDFIKISK
                     DRSLGLTARQKIFLQFIIGLVLGLYVYFYLPEARNILIPFIKIDFNMGAYIIPVIILT
                     YLSAVNAVNLTDGLDGLAASITAVVALTLYIILFFSFFNQVSLLSLSITGACIGFLWY
                     NSKPASIFMGDVGSLSLGAALAVLFSYTGLEIVLIFVGGIYVLEALSVIIQVIYFKVT
                     KGERIFLMSPLHHHYELKGLSESKIVYRFTIVSIMFSLFSILITI"
     gene            complement(3568..>4008)
                     /locus_tag="VKN64_05945"
     CDS             complement(3568..>4008)
                     /locus_tag="VKN64_05945"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMA59818.1"
                     /translation="KLTGLRSEIKRLDGITFINDCYNANPLSMKNAVKMLKNIEGNKK
                     IAVLGDMLELGDIENEAHLEVGNYIADKKVDFLLTTGELGKFIAEGAQNKGMDKENIF
                     HFEDKTKISEKLKEISRTNDVILLKGSRGMRMEIIYQDFSKDKE"
BASE COUNT         1304 a          790 c          571 g         1343 t
ORIGIN      
        1 ccagttattt tcactcaact tatcggctac cgataaagca ggatatatat gacccccggt
       61 accgccccct gtaattactg ctttcatgat ttaccaccta agattcttga ttttttgata
      121 tgtttagtaa gataccgact gaaaataaga acattactaa agaacttccc ccataactga
      181 taaagggaag ggttatacct gttatcggca ttgaggctgt cactacagca atatttaaaa
      241 ttgcctgcac aataatcatg acagtaatac ccatcgctaa tattgagcca aacctatcct
      301 tggtattata gcttatcttt aatcctttcc aggctaccac taaatataat agaattacca
      361 ggagagtagc taaaaaacct aactcttccc ctattattgc aaatataaag tcggtacccg
      421 gttccggtaa atataaaaac ttttgatgac tattacctaa acctacccca aataggcctc
      481 ccgaccctaa tgctaataag gactggataa tatgatagcc ggtatccaga ggatctgacc
      541 agggatctaa gaaagctaga aatctttctt tccggtaagg ttccgaataa ataaaataga
      601 aaactgccgg tacactgacc atacctaacc ctattaaatg ccatatcttt gctccgttag
      661 caaagagcat cacaaagaca atagctaaaa ttagggaggc ggttcctaaa tcgggttcta
      721 ttaagataag cataaatata ataccggtaa caattaagat cggtaaaata ccttttttaa
      781 aacttttaat caaattattc tttctggtgt aaaactgggc aatatacatt atcaaagcca
      841 gcttagcaac ttcagaaggt tgaccaccta aacccgcaat tcctaaccac cggcgggaac
      901 ctcccacttt ttttccgata ccggggatta gaacaagagc cagtaatatt attgcaaata
      961 gtataatata tttggcatat ttttcaaata tgtgataatc aatattcata acaattgcca
     1021 ttgcaataag accgatgaca gcaaatacgg cctgtctttt taaaaagtaa tagctgtcat
     1081 taaacatatc ttctcctctt atagaactgg cactgaatat cattataata ccaatcgcta
     1141 agagagttaa tactgcaaat agcaatataa aatcagcaga tttcttattc attctggtca
     1201 ccttcttttt taactcattt taaaaatttt tgggcttctt gttgaaagtc gagacctctt
     1261 tttttataac tttcgtacat atcccaactg ggacagccgg gtgataataa aactgtatca
     1321 taagttttta tctcattaaa agcttcctgt accgcttcct gcatattatc aactaattta
     1381 tagttattat aatttaaatt gtctaattca cctttgattt ccttttttgt ttcccctaat
     1441 aagataactt tttttacccg ttgttttact gcataagcaa aatccgcaaa atttgccttg
     1501 cgatcctgcc cacccgcaat taaaattatg ggcatctcta tcgaccgcat ggcattaata
     1561 gtagcatgcg gattggtcgc tttcgaatca tcaatcacct tatattcttg gtcataaatt
     1621 acttctaagc ggtgggcaaa agcctgatag ctctgtactg ccttttttat cttttcatta
     1681 tctatataaa aaagtttggc aatcaaaaca gcaaaggcag tatttaagat attatgactg
     1741 cctatcaaag gtatatcttt ggtttcaagt attttactct tacctgcaac tgatttttgt
     1801 atttggtaga tgcttttttt attataaaaa atcccctgtt ctatatcaaa attatttgtt
     1861 actttaaagg tatttgactt tatatcatct aaattatctc ttaaataatt atcatcataa
     1921 tttattactg ctatatcatc agaattctga ttttcaaaaa ttctcttttt ggcattccag
     1981 taattttcaa ccgtattatg tctatcgaga tgatcaggag aaaaatttaa aaatactcca
     2041 atgtggggtt taaacttttt tattttttct aattgaaatg aacttaactc cactactaac
     2101 caatcgtttt cacttaatcc ggttatttct gtaaccaaag gagagccaat attaccagct
     2161 actctaacct tgcagtccaa gttttgtttc aaaatatggc ccgttaaaga ggttacagtc
     2221 gtcttgccat tagtacccgt gataccaatt atattggcat cagtaagtcg ataggcaagt
     2281 tcgatctcgc ttatgacctc aatatttctt tggaccgctt tttttataat atctagatca
     2341 gtgggcaccc cggggcttaa gactattaaa tcagtatcaa gtatctcttc gctgtgacct
     2401 ccgaaatcat atgtaatatt gtcatattta cttaggatag ataattgggg ttttaaatca
     2461 tccttacttt tgatgtcact tatgataaca tctgccccta aatctatcat cttttcggcc
     2521 actgccaggc ctgttctttc acttaatcca attatagtaa ccgttttatt aatgaaattt
     2581 tccatagttt ttaactccta tatcgttatt aatatactaa ataaagaaaa catgatagaa
     2641 acaatcgtaa acctgtacac tatcttgctt tcacttaacc cttttaattc ataatgatga
     2701 tgtaagggag acattaaaaa gattctttct ccctttgtaa ctttaaagta tataacttga
     2761 ataatgacag aaagggcttc tagaacatag attcccccca caaatattaa gactatttcc
     2821 aggcctgtgt atgaaaataa gactgccaag gcagccccga gagacagtga accgacatct
     2881 cccataaata ttgaggcggg tttcgaatta taccataaga aacctataca ggctcctgtg
     2941 attgaaagtg ataataatga tacttgattg aaaaatgaaa aaaataaaat gatatataag
     3001 gttaaggcaa ctaccgctgt tattgaggcc gctagaccgt ccagcccatc cgttagattg
     3061 actgcattaa ctgccgagag gtaggttaga ataataacag gaattatata ggcacccata
     3121 ttaaaatcaa tcttgataaa cggtattaaa atattccttg cttctggtag ataaaaatag
     3181 acatatagtc ctaaaactaa tccaataata aactgcagga atattttttg acgggctgtt
     3241 aaacctaagg acctatcttt acttatcttt ataaaatcat cactaaaacc gataattgct
     3301 gttgagacta atattaatat aaaaataata ttattaattg aaaaggaacc tgttaatata
     3361 accggtatta taattccggt cacaatcaag aggcctccca tggtgggaat accctctttt
     3421 tcataatgtg attcaggccc tacttcccta acattttgcc ccaactcata tttttttaat
     3481 aattttataa agtaaggagt taacagaagt acccaaagga ggggagctgc aaaaaatatt
     3541 aatgatttca ttaaataaga cattatatca ttccttatcc ttactaaagt cttgatatat
     3601 aatttccatt ctcatcccac gtgaaccttt cagtaatatc acatcatttg tgcggctgat
     3661 ctcttttaac ttttcactaa tttttgtttt atcctcaaaa tggaaaatat tttctttatc
     3721 catgccctta ttttgggcac cttccgcaat aaatttaccc agttcaccgg tcgttaataa
     3781 aaaatccacc tttttatcag ctatataatt accaacttca agatgagctt cattttcaat
     3841 atctccaagt tccagcatat ctcctaaaac cgcaattttt ttattacctt ctatattttt
     3901 caacatttta actgcattct tcatagaaag tggatttgca ttgtaacaat cattgataaa
     3961 ggtaatacca tctaaccttt ttatctcaga cctcaaaccg gtcaattt
//
LOCUS       DATGCJ010000123         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_237625, whole genome shotgun sequence.
ACCESSION   DATGCJ010000123 DATGCJ010000000
VERSION     DATGCJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237625"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VKN64_05950"
     CDS             <1..570
                     /locus_tag="VKN64_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HMA59819.1"
                     /translation="QKAVDEGTEPNEIINKGLINGMNIVGKRFKAGDMFVPEVLMSAK
                     AMKAGMNIVKPLLLEGEMPSEGKVLLGTVSGDLHDIGKNLVGMMMESGGLEIVNLGTD
                     IEPDEFAQQVREHEPDVLGMSALLTTTMLAMRDTIEILEEEGLRDSVKIMVGGAPVTK
                     EFADEIGADGWAPDAASAKDLAFDLISEK"
     gene            592..2043
                     /locus_tag="VKN64_05955"
     CDS             592..2043
                     /locus_tag="VKN64_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HMA59820.1"
                     /translation="MQSNYRSKATHNLEVLSEDQIKRILGAAKEILERTGVVYYDDEA
                     IETMKKAGCFVDGNRVRIPTRLVEWALRTVPSKVTLSNSRTGKRVMELTGNNAYFGTG
                     SDTPFFIDPYTQDRIPASKETVAMATKTIDALENMDFVMSLGIVQDVPQLVYDRHQFQ
                     AMVNNTSKPIVITANDVEGYADIIEMCEVIAGGEEELRRHPFMTLYAEPISPLQHAED
                     AAQKLILAGKKQLPVVYTPCIMAGGTVPATLAGAMANGLAESLSGLVLNQLTNEGNPF
                     IMGGVFTIMDMNTTIFAYGAPEFDLMMAALADVSNYLDLPMFGTAGCTDSKVVDEQAA
                     IEDALSILMTAQSGANLNHDVGYIEHGNASALENLVINNDLIGFARRIVQGIEVNEDT
                     LAVDLIDKVGPGGHFLGEEHTMKYFKEETYYPDLFKRQFFENWEEEGRKTLFDRANEK
                     VIDILENYEPEPLPKDVQKKLQDIVDRAESKLD"
     gene            2072..>3741
                     /locus_tag="VKN64_05960"
     CDS             2072..>3741
                     /locus_tag="VKN64_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HMA59821.1"
                     /translation="MANLALGIDTGGTYTDGVIFGMDSREIYSKTKVLTTRHDLSIAI
                     ENCLEGLLDNMEQELKNDDIKMVSLSTTLATNAIVEGQGAEVGLITIGREIEDKIPTP
                     YYFNLAGGCNIKGKIKKEVDLKRARIEVDRLKSKVDAFAVSGYLSIRNPKQENEVKKL
                     IKEVTGYPVVCAHELSSELGFKERTVTAVFNARLMPLITKLIQAVKASLDKRDIEAPL
                     MVVRGDGSLINEEKAREKPVETSLSGPAASIIGAKTLTDITDGIVVDMGGTTTDIAIL
                     KDGKPRLTKKGAKVGGWLTRVKAADITTIGIGGDSFIQVSKDRVLSIGPQKVFPLSWI
                     VKKHDHLHHELSEIKERNYYPLSSQPTTILTYVKDPENLELTDTEKEILKLIREKPHT
                     LYYISKKLDKSPDILPWDHLVKVGSVHRASLTPTDLLHIKGNYKQWDVDAANLGIRIM
                     ADRYETDIETFMQDVFKEIYYQIGSVITEVLISEEGQEISLKHSDSKYFLKKMINHIN
                     QKEEKVKFNLKCNLPMIAVGAPVEAYFPQIAERMNTELILPDYAEVANA"
BASE COUNT         1254 a          671 c          863 g          953 t
ORIGIN      
        1 caaaaagcag ttgatgaagg tacagagccc aacgaaataa ttaataaggg cttaataaat
       61 ggaatgaata ttgtaggtaa aagatttaaa gcaggcgata tgtttgtacc agaagttctg
      121 atgtctgcga aagcgatgaa agcaggtatg aatattgtaa aaccactact tctagaaggt
      181 gaaatgcctt cagaaggcaa ggtactatta ggtaccgtat caggtgacct acatgatatt
      241 ggtaaaaacc tagtaggtat gatgatggaa agtggtggat tagaaatagt taacctcggt
      301 acagatattg agcctgatga gttcgctcaa caggtaagag aacatgaacc agatgtattg
      361 gggatgagtg ccctcttaac taccacaatg ttagcaatgc gtgatacaat cgaaatctta
      421 gaagaagaag gtttaagaga ttcagtaaaa attatggtag ggggagcccc agttacaaaa
      481 gaatttgcag atgaaattgg cgcagacggc tgggcacctg atgctgcctc agcgaaagat
      541 ttagcctttg acttgataag tgaaaagtaa agaaaggatg gtgaaaagta gatgcaaagt
      601 aattataggt ctaaagcgac acataattta gaagttcttt cggaagacca aataaagaga
      661 attctcggtg ccgctaaaga gatattagaa agaaccggag tagtatatta tgacgatgaa
      721 gcaattgaaa ccatgaaaaa agcaggctgt tttgtagacg gcaatagagt aagaatacca
      781 accaggttag tggaatgggc tttaaggacc gttccttcaa aagtgacttt atctaatagc
      841 agaaccggta aaagagtaat ggaacttacc ggtaataatg catacttcgg caccggttca
      901 gatacaccgt ttttcataga tccctatact caggatcgga ttccggccag taaagaaaca
      961 gttgccatgg ccaccaagac aattgatgcc ttagaaaata tggactttgt aatgtccttg
     1021 ggtattgtgc aggatgtacc acagttagta tatgatagac accagttcca ggctatggtt
     1081 aataatactt ccaagccgat cgttattacc gccaatgatg tagagggtta tgctgatatc
     1141 atcgaaatgt gtgaggttat cgcaggtggg gaagaagagt taagaagaca tccctttatg
     1201 accctttatg ctgaacccat ttcaccttta cagcatgcgg aagatgccgc ccaaaaatta
     1261 attttggccg gcaaaaaaca attacccgtt gtttatacac cctgtattat ggccggtgga
     1321 accgtaccag ctactttagc aggtgcaatg gccaatggct tagctgaaag tctgagcgga
     1381 ttagttctaa accaattgac aaatgaaggg aaccccttta ttatgggtgg cgtatttact
     1441 atcatggata tgaatacaac catctttgcc tatggggcac ctgaatttga cttgatgatg
     1501 gcagctttag cagatgtatc aaactacctt gatctaccga tgtttggtac agccggctgt
     1561 actgattcca aagtagttga tgaacaggct gctatcgaag atgccctgtc gatattaatg
     1621 acagcgcagt ccggggctaa cttaaaccat gatgtaggat atattgaaca tggtaatgcc
     1681 tccgccttgg aaaatctggt tattaacaat gacctaattg gttttgcccg ccggatcgta
     1741 cagggtatag aagttaatga agataccctg gccgttgatt taatcgataa agtcggaccg
     1801 ggcggacatt tcctcggaga agaacacacc atgaagtact ttaaagaaga aacttactac
     1861 ccagatctct tcaaacgtca gttcttcgaa aactgggaag aagaaggtag aaaaactcta
     1921 tttgatagag ccaatgaaaa ggtaattgat atcttagaaa actatgaacc agaaccatta
     1981 ccaaaagacg tacagaagaa gttacaggat attgttgata gagctgaaag taaattagat
     2041 taataaatag taataaaagg gggatctttc aatggcgaac ctggctttag gaatagatac
     2101 cggaggaaca tatacagatg gagtaatctt tgggatggac tcccgtgaaa tatattctaa
     2161 gacaaaggta ctgacgacca gacatgacct cagtatagct atcgagaact gtctggaagg
     2221 ccttttagat aatatggaac aggaacttaa aaatgatgat atcaaaatgg tctcactctc
     2281 tactaccctg gctactaatg cgatcgtgga aggtcagggg gccgaagttg gtttaatcac
     2341 aatcggccgc gagattgaag ataagatacc aacaccctat tacttcaact tagccggcgg
     2401 ttgtaatata aaaggcaaga taaaaaaaga agtagactta aaaagagccc gcattgaagt
     2461 tgaccgtctt aaatcaaagg tagatgcctt tgctgtttct ggttacctat cgataaggaa
     2521 tcccaaacag gaaaatgaag taaaaaaact tatcaaagaa gtgaccggct atccggtagt
     2581 ttgtgctcat gagctaagtt ctgaattggg ttttaaagaa agaactgtga cagccgtctt
     2641 taatgccagg ttgatgcccc tgatcacaaa attaatacaa gctgtcaaag ctagtttgga
     2701 taaaagggat attgaggcac ccctgatggt tgtcaggggt gatggctccc tgatcaatga
     2761 agaaaaagcg agagaaaaac cggtggagac ttctctctcc ggaccggcgg ccagcattat
     2821 tggtgcaaag accctgaccg atataactga cggtatcgtt gttgacatgg gggggacaac
     2881 aaccgatatt gcgattctaa aggatggtaa gccccgcctc actaaaaagg gggccaaggt
     2941 tggtggttgg ctgaccaggg ttaaggctgc tgatataact acaatcggaa ttggagggga
     3001 tagtttcata caggtttcta aagatagagt cttatcgata ggaccgcaga aggttttccc
     3061 tttaagctgg atcgttaaaa agcatgacca tcttcaccat gagttaagtg aaattaaaga
     3121 aagaaactat tatcctctct ccagtcagcc aactacaatc ttaacctatg taaaggatcc
     3181 cgagaatcta gaactaaccg atacagaaaa ggaaatttta aagctgatca gagaaaaacc
     3241 acatacctta tattatattt caaagaagct ggataagtcc ccagatattc tgccctggga
     3301 ccatctggtc aaggtcggat ctgtacatag agcttcttta accccgaccg acctcctcca
     3361 tattaagggg aactataagc agtgggatgt tgacgccgct aatttaggta tcaggatcat
     3421 ggctgataga tatgaaaccg atatagaaac ctttatgcag gatgtattta aggagatata
     3481 ctatcaaatt gggtccgtga ttacagaagt cctgatttca gaagaaggtc aggagatttc
     3541 actaaaacac agtgatagta aatatttttt aaagaaaatg atcaatcata tcaatcaaaa
     3601 agaggaaaag gttaaattta atttaaaatg caacttaccg atgatcgcgg taggtgcacc
     3661 cgtagaagcc tacttccctc agattgccga aaggatgaat actgaattga tcttaccgga
     3721 ctatgccgaa gtagcaaatg c
//
LOCUS       DATGCJ010000124         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_282251, whole genome shotgun sequence.
ACCESSION   DATGCJ010000124 DATGCJ010000000
VERSION     DATGCJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282251"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VKN64_05965"
     CDS             complement(<1..488)
                     /locus_tag="VKN64_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMA59822.1"
                     /translation="MKYKPYMGKMLSIDLSTESYEIIETPKNILEEYIGGKGLGVKLY
                     QKYIEPDTKPLSPDNVIFYLTGPLTGTLAPAFRSCLVTRSPLTNTWLDSYFGGHFGQE
                     IKYAGYDALIIKGKAERLTYITIDENEIKFHNADQLKNIKVSETTKRLKDKHGDNFRI
                     SC"
     gene            complement(485..883)
                     /locus_tag="VKN64_05970"
     CDS             complement(485..883)
                     /locus_tag="VKN64_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA59823.1"
                     /translation="MKKIVVNPDLCTGCEICALICSYVHNEGFNPNRACLRINHFDNG
                     VFNSIEFCQQCEDAVCSKVCPEDALTVDREKGYVTFDEERCIGCGKCVKHCPFDMITI
                     NPATNKAFKCDMCEYCVDYCPNDALGVFER"
     gene            complement(901..2079)
                     /locus_tag="VKN64_05975"
     CDS             complement(901..2079)
                     /locus_tag="VKN64_05975"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HMA59824.1"
                     /translation="MLNIYDIKTFEMPTKLVSGVGSRKKLNELLKELEISKPLIVTDQ
                     GIREVGILDKILKIISKDNFDYQIYDKVSINPDVATVDKGYEFYEDHNCDGIIGLGGG
                     SSIDTAKGIGLLDKHGGSILEYECGKKPLEKRIAPLIAIPTTAGTGSEGTMWAVITDH
                     EREVKYNVGGPLLAPQIALVDPELHLTLPPHITAGTGMDALCHAIECYTSHFAQPLTD
                     SVALLAIEYCADYLRRAFANGDDLEARYYMAEAATLAGFSYGSESAGAVHAMTQSLGG
                     IKPDIPHGQAVACLLGPVMMYNWMGEPEKFRRIAIALGKNVRGMSTRDAALEAVNAVF
                     ELTDDLEIPTLQDFGISKDEIPKLAKAAEEDPQTVGNPRDINKEGYEDIYRYTYELAE
                     "
BASE COUNT          790 a          509 c          430 g          916 t
ORIGIN      
        1 atacaggaga tccgaaagtt atccccatgt ttatctttta atcttttagt agtttcactt
       61 actttaatat ttttaagttg atcagcattg tggaatttaa tctcattttc atcgatcgta
      121 atataggtta atctttctgc tttaccctta ataattaatg catcataacc agcatacttt
      181 atttcttgac cgaaatggcc gccgaaatag gagtcaagcc aggtatttgt taagggggac
      241 ctggtaacca gacagctccg aaaggcggga gctaaagtcc ctgttaaagg cccggtcaga
      301 tagaatatta cattatctgg actcaagggt tttgtatccg gttctatata tttttgatag
      361 agttttaccc ccagtccctt gccgccaatg tattcttcta atatgttttt aggagtttcg
      421 attatttcat aactctctgt acttaagtct attgataaca tcttacccat atatggtttg
      481 tatttcatct ttcaaacacc ccgagtgcat cgtttggaca gtaatcaaca cagtattcac
      541 acatatcaca tttaaaagct ttgttagtag cgggattgat ggtaatcatg tcaaaaggac
      601 agtgttttac acacttacca catccgatac agcgttcttc atcaaatgta acgtagccct
      661 tttctctatc tactgttaag gcatcttctg gacatacctt gctgcagact gcatcttcac
      721 actgctgaca gaactctata gaattaaata ccccattgtc aaaatggttg attctgagac
      781 aggcccggtt agggttaaaa ccttcattat ggacatatga acagatcaaa gcacatattt
      841 cacagcccgt acaaaggtcg ggattaacaa ctattttctt catcttatta cacctacttt
      901 ttattctgct aattcgtaag tatatctata tatatcttca taaccttctt tatttatatc
      961 tctagggtta ccaacggttt ggggatcttc ttccgctgct tttgctaatt ttggaatttc
     1021 atctttgctt atcccaaagt cctgtaaggt gggtatttct aaatcatctg ttagttcaaa
     1081 gacggcatta acagcttcta aagctgcatc acgggtactc attcctctaa cattcttacc
     1141 taaggcgata gcaattcttc taaatttttc aggttctccc atccagttat acatcattac
     1201 aggtcctaaa agacaggcta cagcttggcc gtgtgggata tctggtttaa taccacctaa
     1261 ggactgggtc atggcgtgga ctgccccagc actttcactt ccataggaga aaccggctaa
     1321 agtagctgct tctgccatat agtagcgtgc ctctaaatca tcaccgttgg caaaggctct
     1381 ccgtagataa tcggcacagt attcaattgc taataaggcg acggagtctg ttaaaggttg
     1441 ggcaaaatga gaagtataac attcgatcgc atgacagagg gcatccatac ctgtaccggc
     1501 cgtgatatga ggtgggagag ttaaatggag ttcaggatca actaaggcaa tctgcggtgc
     1561 gagtaaagga ccacccacat tatactttac ttctctttca tggtcggtaa taactgccca
     1621 catagttcct tcactaccgg ttcctgctgt agttggaatg gcgattagag gtgcaatcct
     1681 tttttccagg ggttttttgc cacattcata ttctaagatt gaaccgccgt gtttatctaa
     1741 gagtcctatt cccttggcgg tatcgattga actacctcct cctaaaccaa taattccatc
     1801 acagttatga tcttcataaa attcataacc tttgtctact gtggctacat caggattaat
     1861 ggaaacttta tcatagattt gataatcaaa attatctttt gaaataatct ttaatatctt
     1921 atcaagtata ccaacttccc taattccttg gtctgtaaca atgaggggtt ttgaaatttc
     1981 taattcttta agcagttcat taagtttctt tcggctgccc acaccactaa ctagcttagt
     2041 aggcatttca aaagttttga tatcataaat atttaacatt aataatcacc tttcaaaaga
     2101 atattttata ataaaagata aagattatta ttttattagg cgattatgta ttctattaga
     2161 ttgaaaattg ttgaaattat atgagagagc atacaatgaa ttatcagcat gacttatcat
     2221 atttagaaca cccccatttt tagtactaaa aatcattatt acatttgtga catattattt
     2281 attttaatta ttaggatata taattataca ggtagtttaa aaaaataatt aatattcaaa
     2341 atattagaaa atttgtgttt aaaaaaatag atttcttatt tattttcttc aagaaaatta
     2401 ttttcagtcc ttcaagaaat tttgtgagtc tatttatttt ccttatcaag ttatctttaa
     2461 gatagtttca ataaataaag acgccagtat taactgacgt ctttaataaa atgattattt
     2521 tcttataagt tattatcttt aaatatgatt attctccaat tgttaattga gcactgcgat
     2581 caccggaaat actacagtaa gccattatct ttacttcatc accactgctt agaccgggca
     2641 tataa
//
LOCUS       DATGCJ010000125         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_552320, whole genome shotgun sequence.
ACCESSION   DATGCJ010000125 DATGCJ010000000
VERSION     DATGCJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552320"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="pyrF"
                     /locus_tag="VKN64_05980"
     CDS             complement(<1..642)
                     /gene="pyrF"
                     /locus_tag="VKN64_05980"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMA59825.1"
                     /translation="MIKLKYFIDKLVNNIKKADSRICVGLDPHLDLIPEFLLDKAREE
                     YSDQKEAAAKAVLKFNKKIIDEVVEHTAVVKPQIAFYEKMGLKGLKTLNKTVEYAKEK
                     GLLVLLDAKRNDIGSTADAYAEVYLDNENIDAITINPYLGFDGVKPFLKYKDKGAFAL
                     VKTSNQSAGDIQDIESIKGKKIYEHMGQLVKNWGKEYLGKSKYSNLGAVVGATY"
     gene            complement(617..1915)
                     /locus_tag="VKN64_05985"
     CDS             complement(617..1915)
                     /locus_tag="VKN64_05985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMA59826.1"
                     /translation="MKILIKNGHVIDILEGLDGKYDILIEDGKISQIEDEIKAEKAEI
                     IDAKNKLIFPGLIDMHTHLREPGYEEKETIKTGAEAAAAGGFTTIACMPNTNPTIDNP
                     ATVEYVKSIGDKAIVDILPIGSITKKREGKELAELGFLAEAGVKAVSDDGNPVMNSEI
                     MRRAMEYGDNFNLKIISHCEDLNLASEGVMNEGYYSTILGLKPIPAAAEETMIARDIM
                     LAEFTGASLHIAHLSTKRGLELVKDAKKRGVNVSAEVTPHHFSLTDEAVKGYDPNTKV
                     NPPLRSQEDVKALKTGIAEGVIDIIATDHAPHTYEEKLGEFNYAANGISGLETALALA
                     NTYLIKENIIDYKVLLKLMYEKPIELFELDKVGIKKGKLADLIIFDPDKKWEVRIDEF
                     KSKGKNTPFAGHKLTGKNEITFSSGKLVYDNRGDKAEILY"
     gene            complement(1899..>2536)
                     /locus_tag="VKN64_05990"
     CDS             complement(1899..>2536)
                     /locus_tag="VKN64_05990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMA59827.1"
                     /translation="DVVVIRHSAPGAAEFLANNIEASVLNAGDGAHAHPTQGLLDLYT
                     MKSYKGSFKDMKVLIIGDISHSRVARSNIQGLKELGAEVRVTGPATLIPREIEKMGVK
                     VYKNLDKALEGVDVVNILRIQLERQESGLFPSIGEYRKFYGMNKKRLDSLKNDVLIMH
                     PGPMNRGIEIESEVADSEQSVITEQVTNGVAVRMALLYLLAGREIKDEDLN"
BASE COUNT          687 a          556 c          350 g          943 t
ORIGIN      
        1 ataagtagct cctacaactg cccccaagtt agaatattta cttttaccca aatattcttt
       61 accccaattt tttacaagtt gacccatatg ctcataaatt ttcttgcctt ttatactttc
      121 aatatcttga atatcaccag cactctgatt agaagttttt accaaggcaa aagctccctt
      181 gtctttatat tttaaaaatg gttttacacc atcgaaccct aaatagggat taattgtaat
      241 ggcatcaata ttttcattat ccaaatatac ttcagcataa gcatcagctg ttgatccaat
      301 atcatttctt ttagcatcaa gcagtactaa cagccctttt tctttagcat attctactgt
      361 cttattcagg gtttttagac ctttaagtcc cattttttca taaaatgcaa tctgtggttt
      421 tacaactgct gtatgttcaa ccacttcatc gatgattttt ttattaaatt ttaatactgc
      481 ctttgcagca gcttcttttt gatctgaata ttcttctcta gccttatcta aaagaaattc
      541 tggtattaag tccagatggg gatcaaggcc gacacaaatt ctgctgtcgg ccttttttat
      601 attattaaca agcttatcaa taaagtattt cagctttatc acccctgttg tcataaacca
      661 atttgcctga actgaatgtt atttcatttt tcccggttaa tttatggcca gcaaatgggg
      721 tgtttttacc ttttgattta aactcatcaa tccttacttc ccacttttta tcaggatcaa
      781 agataattaa atcagctaat ttcccttttt tgatcccgac tttatctagt tcaaataatt
      841 ctattggctt ttcatacatt aattttaaaa gaaccttata atcaataata ttctctttta
      901 taagatatgt attggcaagg gcaagagcag tttctaaacc ggaaattcca tttgcagcat
      961 aattgaattc accaagcttt tcttcataag tgtgaggagc atggtcagtg gcaatgatat
     1021 caattactcc ttcggctatt cctgttttta aagctttaac atcttcttga cttctaagag
     1081 gagggttaac ttttgtatta gggtcatatc cttttacagc ttcatctgtc aaggaaaagt
     1141 gatgtggtgt tacttcagca ctgacattga cgcctctttt tttagcatcc ttaacaagtt
     1201 ctaaacctct tttagtactt aaatgggcta tatgtaggga agcccctgta aattctgcta
     1261 gcataatatc acgggctatc attgtctctt ctgcagcagc gggaataggt tttaatccta
     1321 atattgttga atagtatcct tcattcatta ccccttcact tgctaaattt aaatcctcac
     1381 aatgacttat tatcttcaaa ttgaaattat ctccatattc catggctctt ctcatgattt
     1441 cactattcat tactgggttt ccatcatcag agactgcctt tactcccgct tctgctaaaa
     1501 agccaagttc agccagctct tttccttctc gtttttttgt aatacttcct attggcagaa
     1561 tatcaacaat agctttatca ccaattgatt ttacatattc tactgtagct ggattatcaa
     1621 ttgtagggtt cgtattgggc atacaggcaa ttgtggtaaa cccacctgcc gcagcagctt
     1681 cagcccctgt tttaattgtt tctttttctt catatccggg ttctcttaga tgggtgtgca
     1741 tatctattag acccggaaaa atcaatttgt ttttggcatc gattatttct gctttttcag
     1801 ctttaatttc atcttctatt tgagaaattt taccatcttc aattaaaata tcatatttcc
     1861 catctaaacc ttctaaaata tcaattacat gcccattttt aattaagatc ttcatctttt
     1921 atctccctcc cggctaataa ataaagtaat gccattctaa ctgcaactcc atttgttacc
     1981 tgctcagtaa taactgattg ctcactatcg gcaacttcac tttcgatttc aatacctctg
     2041 ttcattggac cgggatgcat aattaaaaca tcgttcttta aactatcaag cctcttttta
     2101 ttcatcccat aaaactttct gtattctcct atcgagggga ataatccact ttcctgtctt
     2161 tccagttgga tccttaaaat atttactacg tctacccctt ctaaagcctt gtccagattt
     2221 ttatagacct ttacccccat tttttctatt tccctcggaa ttaaagtggc tggaccggta
     2281 acccttactt ccgcacccaa ttccttaagc ccctgaatat ttgatcttgc caccctactg
     2341 tgactaatat ctcctataat aagaactttc atatctttaa agcttccttt ataagatttc
     2401 attgtataaa ggtccagtaa accttgagtt ggatgggcat gtgccccatc cccggcatta
     2461 aggacacttg cctcaatgtt gtttgctaaa aattcagctg ccccgggcgc actatggcgg
     2521 atcactacta catctg
//
LOCUS       DATGCJ010000126        52041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_149365, whole genome shotgun sequence.
ACCESSION   DATGCJ010000126 DATGCJ010000000
VERSION     DATGCJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 52041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52041
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149365"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VKN64_05995"
     CDS             complement(<1..1132)
                     /locus_tag="VKN64_05995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HMA59828.1"
                     /translation="MKPAVKFIHTADLHLGSRLQLNTDNEREDTYKEAVYNSFKKIID
                     DAIDENVHFVLIAGDVFDNEARSVKATRFFKNQCERLNNNNIDMFLINGNHDPYRMDG
                     ELLELPDNVKICDSEEVSLFEVKKEDKLIARILGQSYRGKAESRKMYTYYTTPDQSVY
                     NIGLLHTQLDPNNTNYVPVSKNDLMDNDHIDYWALGHIHDKRIINDHIPYIIYPGIPQ
                     GRDIGETGPGGYFLINLEPENTYYQFKEVSEYTFKRIELNLSNLPEATLTELHYYLQD
                     ELEELYSKLDKDIKGYIMRIILKGRTELYEVLRENDEEAINDLLLNVRSHFKNKSPSF
                     WIDNIIDHTSKKIPNIEEIMTEDPFIEEFNSFLHSLKEDNKER"
     gene            complement(1133..1702)
                     /locus_tag="VKN64_06000"
     CDS             complement(1133..1702)
                     /locus_tag="VKN64_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA59829.1"
                     /translation="MSLMQGVEFSHWLFRNFLEDNKVLVDATCGNGKDTIFLAEEVSP
                     QGVIYAFDIQKEAVDITKEKFENIEYEFDIQFIVDGHENIDNYIEQKIDGIIYNLGYL
                     PGSEKDIKTEKYTTIKSLNKAIDMLVEGGLIVIVIYSEHEGGGEEKKAVLDFAVNLDY
                     KSYNVLHYHFINQKKNPPEVVAIKKRSGK"
     gene            complement(1873..1995)
                     /locus_tag="VKN64_06005"
     CDS             complement(1873..1995)
                     /locus_tag="VKN64_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59830.1"
                     /translation="MFNIYKKKVKKERHNHQFNSNNYRDKVIDDLLRNNGFYFF"
     gene            complement(2202..2276)
                     /locus_tag="VKN64_06010"
     tRNA            complement(2202..2276)
                     /locus_tag="VKN64_06010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2241..2243),aa:Arg,seq:ccg)
     gene            complement(2316..4241)
                     /locus_tag="VKN64_06015"
     CDS             complement(2316..4241)
                     /locus_tag="VKN64_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMA59831.1"
                     /translation="MVVVNIDSISKSFGTQEVLNDFSMSVNKGEKVALIGANGSGKTT
                     IFKIINGQEDLQEGNLSLRNDVSVGLLDQIPEFNLENTIYEELLTVFNDLRKMEEKLQ
                     KLEKEISTFQKEDKDHHKLERLMDEYSALSHEYEMKGGYGYENKIIQVASGIGFSKKE
                     LDKKISYLSGGEKSRVGLIKLLLKEPDLLLLDEPTNHLDIDSIQWLEQFLIDYEGAVI
                     IISHDRYFLDQVIERIVEIDNGSDEKYYGNYSYYLEERKRRYEQKMREYKNQQKKIKK
                     LEESIHQLRVWGRSRDSEKMFKRAKAMEKRLERIDEMDKPTLHGKKMNLDLEINRRSG
                     DEVLKVNNLKKSFDDNTILKSLDLDIYWQDKAVIIGANGSGKTTLLDLINGDIEPDKG
                     EIKIGASVKMGYYRQEFEKFDPEDDLVTTLVKSCSMKISEARNALGAFLFEDNDVFKK
                     VKDLSGGEKSRLRLLQLMNGNYNFLILDEPTNHLDLPSREVLEEVLQDYPGTLLVVSH
                     DRYFINKIIDHTYELENKKLKKYLGNYDYYKEKKQEIKEDSNKNKENKESNKKDNYYF
                     RQKERERKERKRKRKLNKIEDTIEEKEKKVKELEHKMADPDNIDDFKKLNSLKKEHQV
                     LKDELEQLYEEWEKYLN"
     gene            complement(4254..5504)
                     /locus_tag="VKN64_06020"
     CDS             complement(4254..5504)
                     /locus_tag="VKN64_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HMA59832.1"
                     /translation="MKVGMYSLFSPIHDEDAISQTLEQFVKDLSKNIEVEEINDEILS
                     SDKKEEYDLITVFVKTGGTENIFKEIFDKLEGPVILVATSLHNSLAASMEILSWVKSQ
                     DRRGKILHGKPTEIAKEMENLIKIKETKDKLANSRLGLIGEPSDWLIDSHIDKQAVQD
                     RWGLEVIEIPIQEVLDEYHNIHEERAQNLINEFTDKATSIEEPSENDLMKAAKVYFAL
                     KNIVNRYDLNSLTIRCFDLVTELETTGCLALSLLNNEGIVAGCEGDIPATVSMMITYY
                     LTGNIPFMANPVSLNIKNNKVKFAHCTVATKSVNDYIIRSHFETGIGVGIQGEIKKGN
                     ITVFKLGGKKLDHIAFQNAQLINNLNSEFACRTQVLLDFDGDDISYFLKNPIGNHHIL
                     VEGNHYEILNQFVESLEFDTILHW"
     gene            complement(5535..5825)
                     /gene="yhbY"
                     /locus_tag="VKN64_06025"
     CDS             complement(5535..5825)
                     /gene="yhbY"
                     /locus_tag="VKN64_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HMA59833.1"
                     /translation="MLTGKQRSFLRGEANNLDPYVHIGKEGLNEAVYEKIDEVLDDHE
                     LVKIRVLDNSLEDINEVAHEVCDQLGAELVQVIGNVFIIFRRNMEEPVYNLP"
     gene            complement(5837..7120)
                     /gene="obgE"
                     /locus_tag="VKN64_06030"
     CDS             complement(5837..7120)
                     /gene="obgE"
                     /locus_tag="VKN64_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000496111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMA59834.1"
                     /translation="MFVDEVTFKVKSGDGGNGVVSFRREKFEAMGGPDGGDGGHGGDV
                     ILKVDEGLNTLADFKYNNFYKAENGTHGRGKNQRGKDGKDLYLKVPPGTLVYNDDTNE
                     LLADLKEEGQEFVLAEGGEGGRGNARFKKSTRKAPKFSENGEKGETVKARLELQLLAD
                     VGLVGYPNVGKSTLISNVSAAKPEIASYHFTTVHPNLGVVSYDEYKSYVMADVPGLIE
                     GAHKGVGLGDEFLKHLERTRLLIHVIDVSGIEGRDPIEDFEKINQELKQYDTDLSKLP
                     QIVALNKIDLKEARENLPEVKKELEDRGYKVFPISAVTGEGIDKLKYYIGQKLESLPE
                     KEKEIKAKEDVLIKPEFMDESEMQLKQLADNKYEVVGKLIDELIEKTDFNNDASVQRL
                     LRIVRHHNIDEIMKKEGIEDGDTVIIGPIEFEYME"
     gene            complement(7199..7477)
                     /gene="rpmA"
                     /locus_tag="VKN64_06035"
     CDS             complement(7199..7477)
                     /gene="rpmA"
                     /locus_tag="VKN64_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMA59835.1"
                     /translation="MSQKKGVGSSKNGRDSISKRLGVKEFDGEFVPAGSILVRQRGTK
                     FKPGLNVGRGKDDTLFSKVDGYVKFERKGRKSRQISVYTEDQVEVLIS"
     gene            complement(7525..7836)
                     /gene="rplU"
                     /locus_tag="VKN64_06040"
     CDS             complement(7525..7836)
                     /gene="rplU"
                     /locus_tag="VKN64_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMA59836.1"
                     /translation="MYAIIKTGGKQYKVKEGQMIKVEKLDVEEGDQVTFDRVLQYSDQ
                     EGYKFGKPYLEGAEVSGKVLEQGKNKKVTVLKYKPKRRYYKKTGHRQPYTKVLIENIE
                     A"
     gene            complement(7969..8925)
                     /locus_tag="VKN64_06045"
     CDS             complement(7969..8925)
                     /locus_tag="VKN64_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HMA59837.1"
                     /translation="MSHFDFEISEDNFRVVTLKAADQNNKNVIKIAPGLGNNMFKYQY
                     NDLDLIYYDPDLPLDHFLNGNPIIYPLPNRVNNCHYEFKGKKYWQEKNSKPIFLHSLV
                     YDEKWSYKEPEILADHIKLETFIYIDKDHPVYQGFPFEHQLKIIYKLFNNKLRLKYEV
                     NNKDSKELPYGICFHTYFNYLKQRANSYIKAPAETYMELDADQIPTGKLLGVEETDYD
                     FNDYRSLKDINIDGAITELQDKPKIKFEDKLIIEFDYSKEFSHIQFYTPLNKKFFCLE
                     MQTCSADAHNLDAAGFKKAAHLLTVKPNEKSSGWLDYKPKLC"
     gene            complement(8940..10625)
                     /locus_tag="VKN64_06050"
     CDS             complement(8940..10625)
                     /locus_tag="VKN64_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMA59838.1"
                     /translation="MSRQIIINSCIREKRAAIKENNNLIDIMFERDTYDQIVYNIYKG
                     RVKDVLPGMQAGFVDIGLEKNAFLHISDVYPLLNKDQEKRWRKNELSIKEVLQPGQKI
                     MVQVVKEPIGTKGAKVTCKVTLPGRYLVLMPFENRTGISRRIDDTKERNRLKDIAENL
                     IGNKYGLIVRTNTEGKEREIIENDFNFLIHLWENIWKDYKRAKPPELIYKDAELIKQI
                     VRDYLSPDVNKIVIDDEEEYEKLNELAETSVPGSHQKIYLYERETPIFETYQIENEIT
                     KIWQRKVWLNSGGYVIIDNTEALVSIDVNTGKFTGKKNLQDTVFKTNVEAAREIARQL
                     RLRDIGGIIIIDFIDMSIEENQKEVLRVLEEELAKDRTKTALLGLTKLGLVEITRKKV
                     REEIGELFQKDCPYCEGTGLVMSETTMAMNVIRELKKLSKEEKVEALLIELHPDVAAV
                     LIGAGGDKLTELEEELNMDIYIRGNNDYHIENYEIISKGSKQDLKRMALPVNKGETYD
                     IYIEDTHIHNENDGIARIKGYIIIVKNAGHLKEEEVEVKINEVHRTFAKAELL"
     gene            complement(10622..11332)
                     /locus_tag="VKN64_06055"
     CDS             complement(10622..11332)
                     /locus_tag="VKN64_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HMA59839.1"
                     /translation="MYIRAEYEKLDALKYISHLELMNTFRRTFRRAKIPVSYSQGYNP
                     HIIFSMSQPLPVGMVGYHEYFDLELKEKIRFDLMKKQINEKLPGGLRVLDMIEIHEKE
                     KSLQAIVNTARYRYKMSFKKEVQQKEILKEFLGEKEIEITRFRRKKDDRLIDLAPMIH
                     AGQVVEDGIWEFTISTGSSGNVRPQEVIRALKIKSEGSVEEIPLVNIERLGMFIRKKD
                     KLYKPINRQVLKKEVREN"
     gene            complement(11316..13196)
                     /locus_tag="VKN64_06060"
     CDS             complement(11316..13196)
                     /locus_tag="VKN64_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HMA59840.1"
                     /translation="MNVREQIYDRLIEVENPERYIGNEYNIIKKEWDADKIKVLTAFP
                     DAYEIGMSHIGLKIIYHLLNKEDNIICERTFSPWPDMEELLRKEELPLFSLESGRSVD
                     EFDILAFTLQYEMSYTNILTILELAGLNFYSKNRKASDPLVIGGGSIVSNIEPVAPFF
                     DLLFIGEAEEHIVDIINEYDDLKEQGYKKEEILKQLSHKPGVYVPSLYEVSYDEEGHI
                     TNIDPKQKGIKPRIKRQLVSNLDEAFYPEDFLVPYMEIVHDRATIEVARGCTKGCRFC
                     SAGITYRPVRERKKETLLKQIDNILDSTGYDEISLTSLSTVDHSQIEELVKELTKKYE
                     ERKISISLSSLRVDKFSVQLANEVQKVRKTGLTFAPEAGTKRLRDVINKGVSESNLYE
                     AVEAAFKEGWSTVKLYFMIGLPTERKEDLEGIVKMVKNVLKKGKKIRRDNKKRMKPIR
                     INVSVSTFIPKASTPFQWEPMVDKEELKDKINFLRSKLNTKGINFSWNEEDLSLLESI
                     FSRGDRRLSEVLISAYQKGARFDGWGDFFDFDIWLEAFKENNLDPDFYLRRRNKDEIL
                     PWSHIDMGLAPKFLKREHKKALKNETTSDCRFANCKGCNICINLDVDLDIIGNDYNVY
                     KS"
     gene            complement(13226..14362)
                     /gene="rodA"
                     /locus_tag="VKN64_06065"
     CDS             complement(13226..14362)
                     /gene="rodA"
                     /locus_tag="VKN64_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMA59841.1"
                     /translation="MHFEKKLFKNLNLYIPITVILLIVIGMFAISSAVELNKTGSSGS
                     IFLKKQIIAAVLGVITAIVIQFFDYRIFKEYAQLIFGSMIIVLFMTHLYGANIAGGRR
                     WLNLGFISIQPSELAKIALILVLATILDQRKGLNSFKDFILPFGYFLIPFLLIVFEND
                     LGTALVLLVIFVVMLFVAGANKKFMYVFFGGGFFGTIGVIASHIFLGTPLPFLKEYQL
                     NRLIAFVNPSIDPQGIGYNIIQSKIALGSGRLTGKGLFAGTQNQLNFLPEKHTDFIFS
                     VIGEEFGFIGVFIVIILFLLLFWQLFNIAFQAKDRYGKLVVTGIISMFFFHVVENIGM
                     TMGVMPITGIPLPFISYGGTSMVTSIVAIGIALNVNIRKKKLHF"
     gene            complement(14376..14651)
                     /gene="minE"
                     /locus_tag="VKN64_06070"
     CDS             complement(14376..14651)
                     /gene="minE"
                     /locus_tag="VKN64_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HMA59842.1"
                     /translation="MTIGLLEFLGFKKKKKKDESSKNVAKERLQFILVQDRIKLSPSE
                     MESLKKDLLKVMKKYVDVDDQEIEMEINREEQMMAMVANFPLRRNNQ"
     gene            complement(14664..15458)
                     /gene="minD"
                     /locus_tag="VKN64_06075"
     CDS             complement(14664..15458)
                     /gene="minD"
                     /locus_tag="VKN64_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HMA59843.1"
                     /translation="MAGKSLVITSGKGGVGKTTTVANIGTALAMMDYKVCLIDADIGL
                     RNLDVVMGLENRIVYDIVDVVEENCRLEQALIRDKRHKNLFLLPAAQTRDKTAVQPNQ
                     MESLVEDLKKEMDYVVIDSPAGIEQGFKNAIAGADFALIVTTPEVSAVRDADRIIGLL
                     EAEGLRNPEVIINRINTDMVSRGDMMNIEDIIEILAVKLKGIVPEDNIIIVSSNRGEP
                     VVLKENPKAGQAFKNIARRITGEEVNFISLEDNSFFTKLKKIFSFS"
     gene            complement(15480..16094)
                     /gene="minC"
                     /locus_tag="VKN64_06080"
     CDS             complement(15480..16094)
                     /gene="minC"
                     /locus_tag="VKN64_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HMA59844.1"
                     /translation="MKSPITFKVAKDGIVLTLDPEANFNRIKEALRDHVNSATNFYAG
                     ADLYINIAESSFKIDQLNEIVNIARGYKDVKNIIFTSDVVKRDTVEVNNEGETYLINR
                     TLRSGQKIKKSGNVVIIGDINPGAEVIAGGNIIVFGKIRGVVHAGASGSTEASVTALK
                     LEPTQLRISKKIARPPEESSNKKSLKPERAFIQDGQIMVEDFEI"
     gene            complement(16154..18133)
                     /gene="mrdA"
                     /locus_tag="VKN64_06085"
     CDS             complement(16154..18133)
                     /gene="mrdA"
                     /locus_tag="VKN64_06085"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMA59845.1"
                     /translation="MEDRIKVFKIILIIIFVLLIARAFQIQVLNGDYYYNLSEGNRTS
                     FRPISAPRGKIIDSDGDILVNNKLSHNLYLLPNEIPPQVKVDSLLSSVANLTSMSYEM
                     LQNNYNFSKQKMNNQNALILKRNINKEDVVILLENQDELPGILVKESSMRDYVYKDLA
                     AHIIGYVGEISKAELRHYQSQGINSYHVQDMIGKSGLEKEYESYLKGVDGIRQIEVNN
                     LGEMVKELGTKPPVAGNNIVVNFDLDYQKEVEKLLKEHIERLMKEAEEDPERYEPTGG
                     SAVVLNPNNGKVLAMASYPDFNPNRFSTGLTSQEYQVLSNNPMKPLLNRNIMAAVPPG
                     SLFKLVTGSAAVRFLNVNAETEFVDENGLFYIPGWSRPFKNWHPGGEGELDFTKAIAR
                     SNNIIFYKLGYRLYEEHQGEKLVSTALDYGMGQKTEIDLPEEKKGIVPRGALDGNSGN
                     WYPGDSVNLSIGQGGLLTTPIQLVNYIAAIANKGTLYQPFLVDKIKDANGDIVYDQNS
                     KVIRKLDYGDKLFEILHEGMQEVTMSSYGTARSTFRDFPINVAGKTGTAQTGTEDVSH
                     GWFGGFAPAENPEIAVLVFLENGSSSTYTLPIAGDIIKEYFGIETEVENGESEQTEQT
                     TDEDTEQDNSEQNQTTEQEENGNLLEYIRDVFSSD"
     gene            complement(18123..18623)
                     /gene="mreD"
                     /locus_tag="VKN64_06090"
     CDS             complement(18123..18623)
                     /gene="mreD"
                     /locus_tag="VKN64_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HMA59846.1"
                     /translation="MNRNIIFVLILIIGLVLQITILSLYTPWDIKPDLILVLIVVISI
                     LDGPKTGLLMGIIGGLLQDIFLGEFISVNTIIKAPLGFISGFIEGHFFKGNYLLAPFI
                     VFIASIANYFLTIVLSEELIFSINYWENFRSVIIPSSIYNAILALIVYFILYRLFYYG
                     ENYYGR"
     gene            complement(18635..19459)
                     /gene="mreC"
                     /locus_tag="VKN64_06095"
     CDS             complement(18635..19459)
                     /gene="mreC"
                     /locus_tag="VKN64_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMA59847.1"
                     /translation="MNNKGRLIFAAILIVLLALIGTLYFFNINISYFQYIESGIYNLV
                     SPLIEFFSGIYYETVSYWHGLLNVDEVMEKNKRLERELASLKLQNLMLKGFQRENERL
                     RKLLSFKEHVSFETLGSTVIGFSPSLWENKLIINRGSNDGVRKKMPVISYNGALVGRV
                     DYVGSSSSQVSLIYDPEFVVGGIVQRNESRTIGIVKGQLNNNKMNIMEKISWDADIQK
                     GDIIVTSGFSNNYPKGLPIGKVQEINPDNYGLSQSADIKLFSTINTIEEVLIITDF"
     gene            complement(19472..20509)
                     /locus_tag="VKN64_06100"
     CDS             complement(19472..20509)
                     /locus_tag="VKN64_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMA59848.1"
                     /translation="MFNFLSAPFSRDMGIDLGTANTLVYVKGKGIIIREPSVVALKEK
                     DKKILAVGNEAKNMIGRTPGNIKAVRPMKDGVIADFDVTETMLRHFIRKAHKRNRLVR
                     PRIIICVPSGVTEVEKRAVIDAALQAGARSAFLIEEPMAAAIGAGLPIHEPTGNMIVD
                     IGGGTTETAVISLGGIVTSSSIRIGGDEMDEAISQYIKKKYKLMVGERTAEQIKIDIG
                     TATGDNGGLTKEIKGRDLVNGLPKTVTINSEEIKDALKEPVNNILDAVKRTLEKTPPE
                     LASDVMGRGIILTGGGALLHGIDELLIDTTQMPVYLAEEPLDCVVNGTGNALEELDEI
                     KDLLITPKKLS"
     gene            complement(20533..21192)
                     /gene="radC"
                     /locus_tag="VKN64_06105"
     CDS             complement(20533..21192)
                     /gene="radC"
                     /locus_tag="VKN64_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMA59849.1"
                     /translation="MPVDKRPREKMIKYGSNSLTTEELMALIIRTGYQRETAIDLASN
                     LLNKSKGLKGIIDFTPEEFMQYKGIGKAKSTQLCAVIELSKRISSCNYQGEILLNSPK
                     EAVSLLMPMMRHLKQEHLYALLLDVKNKIIAKTLISKGGLASSTVHPREVFKKAIKKS
                     CASLIIAHNHPSGDPTPSDNDIEVTKRLLSAGEVLGIDLLDHLIIGDGKYISLKAKNL
                     F"
     gene            complement(21269..21850)
                     /locus_tag="VKN64_06110"
     CDS             complement(21269..21850)
                     /locus_tag="VKN64_06110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMA59850.1"
                     /translation="MIDKKIILASSSDRRKELLTRLGIKYTAMPSKIDEDGYDYEHPD
                     KLVQELSLAKASNVANVVENALIIAADTVVAHNNKILGKPEDEEDAKRMLQLLENDKH
                     EVFTGLALISADDEMHFLDYDVTEVFMRKVEKEEIERYIKTGEPMDKAGSYGIQGKGG
                     IFVNKIVGSYFTVMGLPIHLLSMALKSFSIEII"
     gene            complement(21850..22368)
                     /locus_tag="VKN64_06115"
     CDS             complement(21850..22368)
                     /locus_tag="VKN64_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HMA59851.1"
                     /translation="MRTRKIVIIALLVGLGLVLHLVENMLPLSFIIPGAKLGLANIAN
                     LLGLIIFGFIAGLQILLLRIFLGSILAGTFMTINFYLSLTGGLLGYLIMSLFFYYFRN
                     KFSIIGVSLGGAVFHNVGQIITAYLIISNDAIFYYLPYLILLALPTGVGIGLIVIFTY
                     NYLPRGLKRIDV"
     gene            complement(22473..22892)
                     /locus_tag="VKN64_06120"
     CDS             complement(22473..22892)
                     /locus_tag="VKN64_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HMA59852.1"
                     /translation="MEKINKYLTFYDRILIVIIIVVSLIMIAYPFISAYFNNNEDTES
                     YIYIKDSSQEITRIPFSDTYQEEPIIIEVTGPLGTSVIEAHNGRVRLKEAPEEDPAKI
                     CEKTGWISKPGPSIICVPNKVSIWIEKSNSELDAVSW"
     gene            complement(23038..24048)
                     /locus_tag="VKN64_06125"
     CDS             complement(23038..24048)
                     /locus_tag="VKN64_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HMA59853.1"
                     /translation="MDPVYLYSILSMGGIGAVLALGLGIASKKFHVEVDERIEEVEEV
                     LPGANCGACGYAGCASFAEAVVEGDAEISGCPVGGEVVAEKIAEILGVEGENTNETVK
                     AQLLCGGGIKETKKLSEYEGIKTCKAAESINGGTKSCQYACLGFGDCAAVCPVDAITM
                     SDNGLPIIDKETCIGCGKCVEACPKNIITMAPEDKLNHIRCSSHEPGKVVSKICEVGC
                     IGCSLCARACPVDAITMEDNLAVIDYDKCINCGICSEKCPTGTIEFNGRLIEKVTITE
                     DCVGCTRCAKACPVDAIEGELKEVHEIDQDQCVQCGLCVEVCKVDGAINVIYKDEVED
                     ED"
     gene            complement(24073..24663)
                     /gene="rsxA"
                     /locus_tag="VKN64_06130"
     CDS             complement(24073..24663)
                     /gene="rsxA"
                     /locus_tag="VKN64_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HMA59854.1"
                     /translation="MEELRVVVLVFISTVLINNFILVKFLGICPFLGVSKQVETAFSM
                     GMATTFVMTLTAAATWLINTYILEAFNLPFLRYVSFIIVIASLVQFVEMFIRKSSPVL
                     YKALGIFLPLITTNCAILGLALLIPLNGYNFITSVVFGLGAGIGFTLAIVLMAGLREK
                     LEFGDVPETLQGVPITLILAGLMAMAFMGFSGLISI"
     gene            complement(24663..25262)
                     /locus_tag="VKN64_06135"
     CDS             complement(24663..25262)
                     /locus_tag="VKN64_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HMA59855.1"
                     /translation="MLTRLFKDFKNGLWRENPIIVLVIGMCPTLAVTNTVENGLAMGI
                     ATSFVLLSSEIVISLFKNYIPNNVRIPSFILIIATFVTFTDYFLKAFFPDIAASLSLF
                     IPLIVVNCLILGRQEAFASKNPTHRAIADALGMGIGFTWVLVVLSAMREFFGMGSIFG
                     IEILGSWYQPMVIMILPAGAFISLGILVGIMNAIKRRGN"
     gene            complement(25262..25819)
                     /locus_tag="VKN64_06140"
     CDS             complement(25262..25819)
                     /locus_tag="VKN64_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HMA59856.1"
                     /translation="MDTKISKLVLTLTVIGIISAVALSFVYQWTNPHIQQHQAEARQR
                     AIRAVIPEAEEFEEVEKKGNTFYEGFDSGGNRVGVAMIASGPGYQNVIEIMIGVNINE
                     EKIYGIKILSHEETPGLGARITGEEYKSNFVDKPFGDYTVVKRDTQTDLEVEAIAGAT
                     ISSRSVTKIVENAVKQIQDAYGGGA"
     gene            complement(25819..26727)
                     /locus_tag="VKN64_06145"
     CDS             complement(25819..26727)
                     /locus_tag="VKN64_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HMA59857.1"
                     /translation="MNNEQLLVTPNPHIKDATSVEEIMWTVVYALIPAVLAATYFFGF
                     RVLLIVAVSILGAVLTEYIFLKVRKKEIVINDGSAVLTGLLLALTLPPSIPLWTAFLG
                     SVVGIGLGKQVFGGLGYNVFNPALIGRAFLTASYPVLMTTWTVDGTSTATPLSQMAGG
                     EIVRIWNLFVGSTGGSLGETSSLALLLGAAYLIYKGYINWRIPTGMISAVIIMTFLAG
                     ANPVEHLFAGSLILGAFYMATDMVTSPVTKRARWVFGIGAGLLVAIIRLWGGYPEGVM
                     YSILFMNTTVPLLDRYLRPRTLGVVK"
     gene            complement(26739..28064)
                     /gene="rsxC"
                     /locus_tag="VKN64_06150"
     CDS             complement(26739..28064)
                     /gene="rsxC"
                     /locus_tag="VKN64_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HMA59858.1"
                     /translation="MSVLTFKQGIHPEYHKELTKEKPLRDAKRPQEVVIPLQQHIGAP
                     LEPLVEKGDHVKRGQKIGDAEGFVSAPVHASVSGEVKAIENVRTPSGQKTLSIIIEAD
                     EEDEVDDSLKPDKAIEDLTSEDIRHVVREAGIVGLGGAMFPTHVKLAVPDDKEVEYFI
                     LNGIECEPFLNVDNRMMIERSSDIVEGMKLMMKAAGVEKGFIGIENNKPRAISNMKKA
                     VEEEDNIDIEVFKTKYPQGGEKMLIKAALGREVPAGGLPLDVGCIVNNVATAVAVFEA
                     VNEGKPLIERTITVTGEGIMEPCNLYYRIGTRIKDLIEEAGGFEGDPAKIILGGPMTG
                     SAQHSTEVPTVKGTSGIIVLHKDSVAKFDPKPCIGCARCVDSCPMYLLPLKLSDLSQY
                     ERYDELDEYNVQSCIECGSCSYICPADRPLLDYIRIGKMEDMKLKKSSQ"
     gene            complement(28147..28674)
                     /locus_tag="VKN64_06155"
     CDS             complement(28147..28674)
                     /locus_tag="VKN64_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMA59859.1"
                     /translation="MAKKKKSNNRSLTLVIIVMAIICVFVGYLVGLNLFRWMKGGPEQ
                     VAQTENESQQEAQQEESSNEVDQTTEQSEPETESQQQEATEDNTSETAQTEQTEEVSS
                     GNYSNVYKIQVGAFSQRSNAESFKKELEDEGYNVIIKEASTFKVRVIGEESREATEQI
                     EEELIELGYDTFIVK"
     gene            complement(28667..30019)
                     /locus_tag="VKN64_06160"
     CDS             complement(28667..30019)
                     /locus_tag="VKN64_06160"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HMA59860.1"
                     /translation="MLKRNGNMNPYEFLLQFKKYGKKGGFQPGLERVNKLLSYLDNPQ
                     DKVNYIHIGGSNGKGSTAAILTSIFKEAGFKVGTYISPPLVHFNERFKVNEIPITTNE
                     LKEIVSELKDIFADPAKDIKIEEPSFFEIITVIAFQYFYKKEIDIGILEVGLGGRLDA
                     TNAVSSPLLSIITNISYEHADILGPEIKDIAFEKAGIIKKGVPVITGAENKEALDVFK
                     KIAAQMKAKLTIMEKAANYELIDKNLKQQTFNLRYNGETFDDIKLNILGEHQVKNAAL
                     AVRSLNLLPARYKINKEKIYKGLDRCRWPGRLEMMNRNPNIILDGAHNVDGMKRLVQF
                     IDDNIKKDKKLFFLISILKDKDYKEMFNIINTLDHKMELSITKNQNERSLKPTLLKNY
                     ADTLNLKNKVYNNIQKAVKQTYKTMDEDDTFCITGSLYTVAEARFFTHLLIEGGLKDG
                     "
     gene            30095..30529
                     /locus_tag="VKN64_06165"
     CDS             30095..30529
                     /locus_tag="VKN64_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HMA59861.1"
                     /translation="MNKILIVSLISLFLAQFLKIFTIKPSSFTNLFGSGGFPSSHSSF
                     VATLSTLIGLKHGFNSDLFAIVLIFSLIVTYDASGVRRAVGEQANVLNNLIQHLNVKN
                     IERDPEILKEDLKELIGHTPLEVFAGVLLGVSVAFISYYYFF"
     gene            complement(30526..31782)
                     /locus_tag="VKN64_06170"
     CDS             complement(30526..31782)
                     /locus_tag="VKN64_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59862.1"
                     /translation="MKYLSLLKYITGVNKIYIVGGAVRDYLLNKEIKDIDLIVMSDFE
                     NIIDRFTNETDRKLIVLDKKRSIYRIVTDDNKFIDFTTPVGQDLYQDLGCRDFTINSM
                     ALDLRDVSYKNEKIFMDSKDIIDPFEGRSDLENKIIRMVSEDSIKEDPLRILRAFRFS
                     NNLDFLIEEKTYEIIKENINITLKPANERIKEELYQLYSNYLKPARIKLLLESNILKV
                     LFGVDLKKDRKIRKKLLKQLHFVKKNRYLENIKIESYLFNLILFFMFLILKNKVTLKQ
                     LEDRLINYTFNKQGIDLVIDHLHFIKNLLRDYKSYLNNDILLYEELFSKSIDIKVSKY
                     LLLSLFHVKKDKKEKELVFKVLNKVAEMQKRTAEKLISGNDIKKVLNIAEGKLVGEIL
                     EDIEGKQALGVFKKREQLLDYIKKNY"
     gene            complement(31798..33768)
                     /gene="tkt"
                     /locus_tag="VKN64_06175"
     CDS             complement(31798..33768)
                     /gene="tkt"
                     /locus_tag="VKN64_06175"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMA59863.1"
                     /translation="MLKEVANIVRGLSVDAVEKANSGHPGMPVGCAEIGSLLYGEVLK
                     HDPASPNWPDRDRFVLSAGHGSMLLYSLLHLSGYDLSLEELKNFRQLESKTPGHPEYS
                     ETEGVETTTGPLGQGFANAVGMAIAERHLASKFNKGDLDIVDHYTYTIMGDGCMMEGI
                     SSEAASLAGHLGLGKLIAIYDDNDISIEGSTDITFTESVPDRFKAYNWHVIEDVNGHD
                     INEMRKALMKAKAEPTKPTLIAAKTHIAYGAPNKQDSAEAHGAPLGEEDIKGLKEFFG
                     LPTDKEFYVSDNVKDYFSERKEILEERKEAWDYKFAEWKEKYPKLYKEWKKAYNLEVK
                     EDLEDIVHNLDIDTPIATRKASGATLRTIADRLDYLIGGSADLAPSNKTYLDKYEEFQ
                     PNSHSGRNFRFGVREHAMGGVVNGMALHKGVRPFAATFLVFSDYMRPAIRLAALMKQP
                     VIYVFTHDSIYIGEDGPTHQPVEQLEALRAIPHLQVYRPADAEETKEAWLKAVKRTEG
                     PTALVLTRQSLPEIAKENGIKNFEKGGYLVRKGKGDYRFTFIASGSEVSLAQKIADKL
                     KEKDIESRIVSVPERSILESQDRAYIKELMGNPKDKKVVIEAGIGSGWYRFLDQDDTV
                     ISQEEFGASAPGKEVAAHFGFDADKIIKRLID"
     gene            complement(33820..34689)
                     /locus_tag="VKN64_06180"
     CDS             complement(33820..34689)
                     /locus_tag="VKN64_06180"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HMA59864.1"
                     /translation="MKNPVPKVAAIHDISGYGRGSLTTVIEVLATMGIQPCPLPTAVL
                     STHTGGFKDYKFIDLTEELPDYINHWESLAIDFDAIYSGFLGSVHQIEIVIDFIKRFK
                     RDEQYVIIDPVLGDNGETYDTFGPKMVNNMRKLVKKADLITPNITEAKFLLKKDKNGI
                     NSDEKAKEWLRDLSSFGPDMVVITSVPGYKSKEKTSVLAYQKKEDRYWKVDCDYIPAN
                     FPGTGDLFTSILVGSILSGDSLPMAIDRAVQFISMAVRTTYGYDFPEREGVLIERVLA
                     NLNLPITGKSYEI"
     gene            complement(34704..35948)
                     /locus_tag="VKN64_06185"
     CDS             complement(34704..35948)
                     /locus_tag="VKN64_06185"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HMA59865.1"
                     /translation="MKTLALILAGGRGSRLDILSEHRAKPSVPFAGKYRLIDFVLSNC
                     VNSGIYNVGVLTQYLPLSLNDHIGIGKPWDLDRKYGGITILQPYTGKEGGWYKGTAHA
                     IYQNMSYLRSQDPEYVLILSGDHLYKMNYDKMVEDHIDRNADLSIAAQRVPQEDASRF
                     GILDTDKTGKITDFLEKPENPPNNLASMGIYVFTKDALIKALNKYCSAEKSDFGHHVI
                     PPMIKNNEVYAYEYEGYWRDVGTLKSFWETNLSLTESIPELNLYDEDWKVHTKSVEKP
                     PVKFGPNSKSKNSLLSNGAIINGIVENSVISPGVYIEENVVVRNSIIFNDTRIRKNTI
                     VDKCIIDKEVDIGENCHIGFGDDLTPNEEKPDLLFNGLNIIAKRAVIPNNVQIGRNCR
                     VMSYTREKDFDNKNIESGSTIG"
     gene            36196..37509
                     /locus_tag="VKN64_06190"
     CDS             36196..37509
                     /locus_tag="VKN64_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA59866.1"
                     /translation="MEKTNLKLLKNKDFILLFLGGFISRIGNGIHYIALVWFVLDITG
                     SGSATGLILLLSTLPGVIIGPFSGVIADKFDRKKLIVYMDILRGLVVLWLGYTVYIGT
                     ANFFHIGLATVLIAVCTSFFNPAVTASIPNIVDDELLQKANSLNHFSINFTQIIGAAV
                     GGILIAIFGIAGVFFINGISFLLSAFSEIFINIPEVKIEEEIEKAPTIINEIKFGFNF
                     LWQKKELVSLFTVALFLNFISTGLIVLGLPFVYKELLGVNSQLYGYAQSIFPAGAVIG
                     SIILSQIPEIRNYFKVLFYSLSSQSMLMIIVAVPLLPYFLANASVMTIYYSLMLILFV
                     VGILNAVVNVPISILLQRLIPDNLRGRITGLLSTMSQALVPISLALTGYLLDLVPVYT
                     LFLGAGIASLGLSLYIAKIPAMQKLNYKEEKVEPNMEVSTNTLDA"
     gene            complement(37487..38530)
                     /locus_tag="VKN64_06195"
     CDS             complement(37487..38530)
                     /locus_tag="VKN64_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMA59867.1"
                     /translation="MVSLLFISSGLFLGWSLGANDAANLFGTAVSTKMVRFTTAAIII
                     SVFVIIGAVVSGSGTTETLGTLGAVNTMPGAFTVALAAGLTVYLMTNYELPVSTTQSI
                     VGAIIGWNIFSGYSTELGVLSKIVGTWVFSPILSALFAMLFFKLAQYIVNNYSLHLFK
                     IDMYTRLGLILVGAFGAYSLGANNIANVMGVFTQTSTLEGIKIGFITLSDTQVLFLLG
                     GISISVGVFTYSKRVMTTVGKSIFKISPVASLIVVLSSSTVLFIFASEGLRSILLSLG
                     LPPLPLVPVSGSQAVVGAVMGIGLAKGGRNVNYKLLGKIGLGWILTPLMALIIAYIAL
                     FFMQNVFMHQVYL"
     gene            complement(38563..39216)
                     /locus_tag="VKN64_06200"
     CDS             complement(38563..39216)
                     /locus_tag="VKN64_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMA59868.1"
                     /translation="MKKFVFNKSKELQKDIDEYLDTVEEALLIFERDINRYFDGHYDL
                     FEENLEDILGIENRADDILKDIKYKLYTYMLIPEARGDVLSLLEDMDNEVDLCKKVLM
                     QFSIEKPEIREFVLAEFKDLAKQSLNSSEELVKGVRTYFEEISMVNDYVNKVHFYEHE
                     ADKIEERIKRKVFNTDEIDDFCKKVHLRHFSERIAYFSDLAEEISEKLSVAAIKRSI"
     gene            complement(39223..40188)
                     /locus_tag="VKN64_06205"
     CDS             complement(39223..40188)
                     /locus_tag="VKN64_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA59869.1"
                     /translation="MDNKKFNIFTNKYSVVLLAIAACILWGSAFPSLKVSYSILELEN
                     SGPFNKMLFASYRFMLASFMLILFIVIKSGIRRLKLNKANYIHLISLGLIQTFAQYAF
                     FYNGLANTTGVKASILVTSGIFFTVIASHFIYIDDKLNKVKTFGLLLGITGVIIINLS
                     RGNLNFVFNFTGEGFIIMSGVMSTIAIFYVKYLSDEIDIMIITAYQMLFGSVTLFLIA
                     LSGADLRSINFDLKSGMLLIYLAFISAAAFGLWYTLIKFNKVGYITIYKFIVPVSGVF
                     LSAFFIISETININAVLALILVSLGIVIINMPQNIFNRKVKAKRG"
     gene            complement(40190..41089)
                     /locus_tag="VKN64_06210"
     CDS             complement(40190..41089)
                     /locus_tag="VKN64_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA59870.1"
                     /translation="MKLAENDIKIYIILAIGVFIISFSGIIIKVTTAPPLIIAFYRVF
                     LTSIFLTPVFFTKYRDSLKFFLDIRPALVGFVLALHFYAWISAVHLTNVANAVIFIAI
                     QPLFTLLLEYLFAKEDVRKGAVAGVIFALIGSIIISFSDLYIILTRFYGDMLAILAAF
                     LAACYLFLGRSFRKEIKYIPYIYILYSYTAFFLFIFVLLSGNSFTGYGNMNYIYILIL
                     ALGPTLIGHSVLNLSIRYVPATIVSLAILGEPIMTTVLAMIILGERLTTATMIGGVLI
                     LYGIFRAINSNQKQKEKIAGGKT"
     gene            complement(41102..43438)
                     /locus_tag="VKN64_06215"
     CDS             complement(41102..43438)
                     /locus_tag="VKN64_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HMA59871.1"
                     /translation="MLTYHRLKSEKPPLYDYEPWRITEQEFEVENNHHNETIFSLGNG
                     YMGLRGTLEEDYTGTSDSTTPGFYINGIYEEEEIIYGEFAPKQPQNYQTMINLMDWKT
                     INLYIEDEKFNMLEGKVEGYERYLDLKKGLLKRSLVWTSPNGKKINIEIERIISLAHK
                     HIGTIKYAVTPLNFNGKIKIESSLIGDVQNSHHLRDRSPLLIEQKGIDSQFSYLMVTT
                     KNSDISIGSTIYNKLMGIEEKFLLREENIEGKNIKEVYEIDAKQDKTYRLEKYAAFYT
                     SRDEIEKEEILAQCYQSAKRAKAMGFDEIKASQIEYLKDYWQYSDVKIEGDEALQQAF
                     RFNALQILQSTGKDGKTNIAAKGLTGEYYEGHYFWDTETYIIPFFLYSHPEIAKQLLN
                     YRYEILDSARDNAERMKLKGTLYPWRTINGEEASGNFLGSTVQYHINADIAFAIKNYV
                     QATGDKDFLYNKGAEILFETARMWLSLGHYEELKEGQFCINEACGPDEYKPAVNNNCY
                     TNYMAKFNLEYAVEVYQELKEENRDKLNKIKKAINLLPDEIREWQESAEKMFLPYNEE
                     LGINPQDDSFLYKEEIDIESIPIDELPLVKNWHPLTIWKYQILKQADVILLMLFMGDK
                     FSLEQKKRNYDYYEPKTTHDSSLSPSIYSIIASEIGYHDDAYDYFKQTARLDLDDYNE
                     NAYQGLHTACMGSNWMVLVQGFAGMRTYEGELIFKPYLPKQWDGYEFKIKYRGALLKV
                     HVKEDEIEYNLLEGDSIQFKHKDREIKLDKDNDHILLN"
     gene            complement(43456..43926)
                     /locus_tag="VKN64_06220"
     CDS             complement(43456..43926)
                     /locus_tag="VKN64_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HMA59872.1"
                     /translation="MVKVALLTIRDSKHNEKKEEETRKIVRNLASQIEGEKVYFKSID
                     NRYEKTKEELFDLSVNREAELILTCGGTGLSPKDLVPEATKDVIEKEVPGIPEKMRRI
                     VSEYRPSACIFRGTAGINKESLIINLPGDPKLVKDCLDSIIDVIPRGINMLKNK"
     gene            complement(43950..44957)
                     /locus_tag="VKN64_06225"
     CDS             complement(43950..44957)
                     /locus_tag="VKN64_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HMA59873.1"
                     /translation="MRQLDERKNNRNKLIIITVLTFLIMFVSLFIGRFNIPFMKFING
                     FNLNEMEQSVFYYIRLPRILLVMGVGAALSVAGASYQSIFRNPLVSPDILGVSAGASF
                     GAALGMILSFQSFSSIYLLAFVFGVIAVFLTYFISNLGKTNMIMMMVLSGIVVGSLFN
                     AFISILKYMADPYEKLPGIVFWLMGGFSRTGWEEVKFAAPFIIIGIIILVLIRWYLNV
                     MSMGEEEAISMGINVKLVRNVMVLFSTLMVAASVATVGQVSWVGLVVPHIARFIVGAD
                     HRYMVPASGLLGASFLLLMDDIARSLTGAEIPISIITALLGAPFFAYLLISQKNSGWN
                     R"
     gene            complement(45017..45721)
                     /locus_tag="VKN64_06230"
     CDS             complement(45017..45721)
                     /locus_tag="VKN64_06230"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HMA59874.1"
                     /translation="MKKIGLLGGMGWYSTSLYYNRINKYINRIRKNNINPEIIIYSIN
                     FDQIVKLLEKDDWDSIAEIMINKANKIEEGGADFLVITSNTIHKIEDEIQNNINIPIL
                     SIIDVVGKNLKKTWCKRPGLLATKYLINNGFYQDELETEFAQKVVTPDPREIDELNEI
                     IINELVCNNINISSRYYINSIIDNLMFQDVDAIILGCTEFSSLIDIIAAREVRIIDSL
                     ELHVREIVHYTLRKNL"
     gene            complement(45718..46968)
                     /locus_tag="VKN64_06235"
     CDS             complement(45718..46968)
                     /locus_tag="VKN64_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA59875.1"
                     /translation="MITISKKSVAVLGAGNRGKDVYGKLIKQNKNLQISAVAEPLEDR
                     RKKFAKEHKIPPNYQFESWEDLLFEERLADGLIISTGDKIHFEPLKKALSKGYKVLCE
                     KPITDNFNELKTLQEDYSEDSEQVMVSHVLRYTPFFKKIKELLNKNIIGRIRFINLIE
                     NIGFFHYGHSYVRGNWRNHEVSAPIILAKSCHDLDILYWLMEVGYKNIYSDSSERYFD
                     KKYDLPQAGERCLTCPVEDNCPYSAKKLYFAVPFGWPVNVITDDFSEEGITKALKETN
                     YGKCVYKSDNDQLDVQTLLLKFKNEITAHFTLTAFSNDMTRKINIFGTHGEIFGDLEK
                     GIIEVKPFLDKHYKINIEYKGGHAGGDKKLIESFAKFLSNKLDKNDSNLMSALESHFA
                     ALAAEKSRKIKSAVKLDEFRGYKK"
     gene            complement(47026..48591)
                     /gene="purH"
                     /locus_tag="VKN64_06240"
     CDS             complement(47026..48591)
                     /gene="purH"
                     /locus_tag="VKN64_06240"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMA59876.1"
                     /translation="MTEIKRALLSVSNKTGIKDFAESLAAQDIEIISTGGTANRIAEA
                     GIDVINISDVTDFPEMMDGRVKTLHPNIHAGILARRDNEADLTELKSLDIKTIDLIVC
                     NLYPFAQTIDKKEVELKEVLENIDIGGPTMIRAAAKNFPDVLVIVDPKDYSLVAKKLK
                     DNQKIDKSFRMKMAYKAFNHTAEYDSIIQKYFFDNLEEKEEWPDILLEPYYKKQKLRY
                     GENPHQSAVFYQARKVNEPSITNAKKLSGKALSFNNINDTNGAIELVKEFQDKPTAAV
                     IKHTNPCGLASADDIITAFKRAHAGDPLSAFGSIVSLNRSVNKELAEEIVSGEKYIEV
                     ITAPDYSKEAVDILTNRWESIRILKTDDLYINHKEPGYELKKVTGGLLMQDRDLEMVS
                     SDDLQLVTEKGVESNLIKDLLFAWKAVKHVKSNAIVMAKEEMLVGVGAGQMSRVDAMI
                     IAGRKAQGRQQGAVVASDAFFPFPDAVEKAAEYGIKAIIQPGGSIRDDQVIQKADELG
                     IAMYFTNIRHFRH"
     gene            complement(48597..49217)
                     /gene="purN"
                     /locus_tag="VKN64_06245"
     CDS             complement(48597..49217)
                     /gene="purN"
                     /locus_tag="VKN64_06245"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMA59877.1"
                     /translation="MNDKIFNLAVLASGRGTNLQSIIDKLHLSEKPIEIKSVISNKKE
                     AYALKRAKNCGIKVYLIDENRYNNKKDYEDKVIEAIQKSNIDLIVLAGYMKVLSARFV
                     NTFKNKIINIHPSLLPAFPGLNAQKQAVDYGVKVSGCTVHFVDEGVDSGPIIKQKAVP
                     VKDSDDEESLADRILKHEHEILPAVIELIAEDKVKLKDDNTVLVKE"
     gene            complement(49210..50217)
                     /gene="purM"
                     /locus_tag="VKN64_06250"
     CDS             complement(49210..50217)
                     /gene="purM"
                     /locus_tag="VKN64_06250"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMA59878.1"
                     /translation="MGLSYKDAGVDIKKGEEAVKQINEMVKSTYNENVVTELGGFGGL
                     FNLDLKEYNEPILAAATDGVGTKLKLAFLADKHDTIGIDLVAMSVNDLVTLGAKPLFF
                     LDYLATGNLDVKQFNEVVAGIVQGCKEAGCALLGGETAEMPDFYPDGEYDTAGFCVGI
                     VDKKEVIDGSEIKKGDTLIGIASNGIHSNGYSLVRKLFIKNNEENEQSLLENLLKPTK
                     IYVKPILELIDKYNIKGIAHITGGGLKENVPRILPTGYTAEIATSTFPTPEIFKKIQK
                     KGNIESDEMYRTFNMGLGMVLVVNKKDIDKVLKTLKELGETAYEIGEIDKGDKGLILN
                     E"
     gene            complement(50201..51640)
                     /gene="purF"
                     /locus_tag="VKN64_06255"
     CDS             complement(50201..51640)
                     /gene="purF"
                     /locus_tag="VKN64_06255"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMA59879.1"
                     /translation="MGNCNREKALKKSDKMEEECGIFGIFSPEKRDDISRLNYLGLHA
                     LQHRGQESAGICVNDENKLRVHKGMGLVSNVFDSDILGDLTGNISIGHVRYSTTGSSL
                     LVNAQPLLTNSIKGDLALAHNGNLINGRELKDNLESHGSIFHSTLDTEVIAHLVARSF
                     KDDIIEALIQSLQQIKGAFSLIAMTNDSLIAARDPHGFRPLILGKKGDSIILSSETCA
                     LDIIGAEFIREVKPGEVLIIDEDGIKSQRYSEDKPKAFCVFEFIYFARPDSIIGGENV
                     YLARKRMGRRLAREMNMEADLVVPVPSSGVAAAQGYSLESGISYADGILRNRYVGRTF
                     IQPTQEMRDLKVRMKLNPINKILEGKDIILIDDSIVRGTTSRQIISRLRETDVNKIHM
                     AIASPPVKHPCFYGLDTSRRKELIARKKETEEIAEYIGADSLHYLSLEGILSVLEADD
                     YGYCSACFNGKYPTAEKEKREVDKNGFIL"
     gene            complement(51610..>52041)
                     /locus_tag="VKN64_06260"
     CDS             complement(51610..>52041)
                     /locus_tag="VKN64_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMA59880.1"
                     /translation="LALKEDKIDGYVPEIDLKLEKRVQRLVREAVKEGIIKSAHDCSE
                     GGLAVALAESCFENELGIDVDLKDNISDKALLFGESQSRIVVSIDPADSELLNEIADN
                     NSIPLKQIGQTKRSIFKFNDLIQLNVKEMKKNWETAIEKKL"
BASE COUNT        15705 a        10680 c         7268 g        18388 t
ORIGIN      
        1 ttctttcctt attatcttct tttaaactgt gcaaaaatga gttaaattcc tcaataaagg
       61 gatcttccgt cataatttct tctatattgg gaatcttttt agaagtatgg tctataatat
      121 tatctatcca aaaagaaggc gatttgtttt taaaatgact tcttacgttt aacaagagat
      181 catttattgc ttcttcatca ttttctctta agacctcata taactcagtc ctacctttta
      241 gaattattct cattatatag cctttaatat ctttatccaa tttgctgtat aattcttcta
      301 attcatcctg caaataataa tgtaattcag ttaaagtggc ttccggcaaa ttacttaaat
      361 tcagttcaat tcttttaaag gtatactctg atacttcctt aaactgataa taggtatttt
      421 cgggttctaa gttaattaag aaatatccac ccggacctgt ttcaccaata tccctgccct
      481 gcggtatacc agggtaaatt atatatggta tgtggtcatt aataattctt ttgtcatgga
      541 tatggcctaa agcccagtaa tcaatatgat cattatccat taaatcgttt tttgaaaccg
      601 gtacgtagtt tgtattattg ggatctaatt gggtgtgaag taagccaata ttataaacac
      661 tttgatccgg ggtagtataa taagtataca ttttacgaga ttctgctttc ccccgataag
      721 attgtcccag aattcgggca attaatttat cttctttttt tacttcaaac aagctgacct
      781 cttctgaatc acagatttta acattatccg gcagttctaa gagttctcca tccatgcgat
      841 agggatcatg gttaccgttt attaaaaaca tatcaatatt attattattt aatctttcac
      901 actgattttt aaaaaaacgg gtagctttaa ctgatcttgc ttcattatca aagacatcac
      961 cggcaattaa tacaaaatgt acattttcat caattgcatc atcaattatt tttttaaagg
     1021 agttatagac cgcctcttta tatgtatcct ccctctcatt atcagtattt aattgtaatc
     1081 gactgcccaa atgcaggtca gcagtatgaa taaacttaac agctggtttc atttatttac
     1141 cgctcctttt tttaatagcc acaacctcgg gaggattctt cttttggtta ataaaatggt
     1201 aatgtaaaac attgtatgat ttataatcta aattgacagc aaagtctaaa acagcctttt
     1261 tttcttctcc gccaccttca tgttcagaat agattacaat tacaattaga ccgccctcaa
     1321 ctagcatatc aattgcttta tttaatgatt ttatcgtagt atatttttct gttttaatat
     1381 ctttttcaga tccaggtaga taacctaagt tatatattat accatctatt ttttgctcta
     1441 tataattatc tatgttttca tgtccatcca caataaattg aatatcaaat tcatattcaa
     1501 tattttcaaa cttttctttt gttatatcca ctgcctcttt ttgaatatcg aaagcataaa
     1561 ttactccttg tggacttact tcttcagcaa gaaatatagt atctttacca ttaccacaag
     1621 tagcatcaac taatacttta ttatcttcca aaaaattcct aaagagccag tgtgaaaatt
     1681 caaccccctg cattaaactc atatttttaa cctccaaaaa atcttattgt atattattat
     1741 ttctggaaaa agaagaaaac acctgtttga tttaatacta aaaaccctta ttatatttca
     1801 ctttttttga aaaaaataaa aaaactgccg ggaaatcccg acagcctttt aaatactctt
     1861 aaatttttaa ttctagaaga aataaaatcc gttgtttctc aacaggtcat caattacttt
     1921 gtctctgtaa ttgttactgt tgaattggtg attgtgtctt tctttcttca cctttttctt
     1981 atagatatta aacattttaa atgtccccct ttcatatatt tggtagggaa catcctcgct
     2041 tcaactctac tttgttccat ttataatttt taaaaactac tctgtatatc tcgtgttctt
     2101 aaggctagta taacttaata taataaaaaa tgcaatacct atttttgttt aattttaaaa
     2161 attctaggat aatttttgtt aattttacac aaaaataaaa atggtacccc tagcaggaat
     2221 cgaacctgcg cactcggctc cggaggccga tgctctatcc actgagctac aggggtctat
     2281 gacaattaag attataatat aattttgcta gaaaatcaat ttaaatattt ttcccactct
     2341 tcatataact gttccagctc atcttttaac acttgatgct cttttttaag ggaatttaac
     2401 tttttaaagt catcaatatt atcgggatca gccattttgt gttctaattc ttttactttc
     2461 ttttcttttt cttcgattgt atcctctatt ttatttagtt tccgcttcct ttttctttct
     2521 tttctttctc gttctttttg tctaaagtaa tagttatctt tcttattact ttctttattt
     2581 tccttattct tattactatc ttcttttatc tcctgttttt tctctttata gtaatcataa
     2641 ttacccaaat actttttaag ctttttattt tccagctcat aagtatgatc aattatctta
     2701 ttaataaagt acctgtcatg ggaaaccacc aataaagtac cgggataatc ctgtaaaact
     2761 tcctctagta cttctctaga gggtaaatca aggtggttgg ttggttcatc caaaattaaa
     2821 aaattataat taccattcat taactggaga agtcttaacc tgcttttttc tcctccactt
     2881 aaatctttta cctttttaaa aacatcatta tcttcaaata gaaaagcacc taaagcattg
     2941 cgggcttcac taattttcat cgaacatgat ttgaccaggg tagtaacaag atcatcttcc
     3001 gggtcaaact tttcaaattc ctgtcggtaa taacccatct tgacactggc cccaatttta
     3061 atttcacctt tatcaggctc tatatcacca tttatgaggt ctaaaagggt tgttttacct
     3121 gaaccattcg cgccaataat aactgcctta tcctgccaat atatgtctaa atcaaggctc
     3181 tttaagatgg tattgtcatc aaatgatttt tttaaattat taacctttaa aacttcatcc
     3241 ccactgcggc ggttaatctc taaatctaaa ttcatttttt tgccgtgtaa ggtcggttta
     3301 tccatttcat caattctttc cagtcttttt tccatggctt tggccctttt aaacatcttt
     3361 tcactatcac gggatctacc ccagacccgc agttgatgaa tggattcttc taattttttt
     3421 atctttttct gctgattttt atactctcgc atcttttgtt catatcttct ttttctttct
     3481 tctagataat aactataatt accatagtac ttttcatcac tgccattatc tatctctaca
     3541 attctctcaa ttacctgatc aagaaagtac ctatcgtgag agataattat gacagcacct
     3601 tcatagtcaa ttaaaaactg ttctaaccac tggatagaat caatatctag gtggtttgta
     3661 ggttcatcta ataataagag gtccggttct tttaataata gtttgattaa accaaccctg
     3721 gacttttctc ccccacttag atatgatatt tttttatcta actctttttt agaaaaacct
     3781 atccctgagg ctacctggat tatcttattc tcatatccat atccaccttt catttcatac
     3841 tcatggctta aagcactgta ttcatccatc agtctttcta atttatggtg gtctttatct
     3901 tctttttgaa aggtactaat ttctttttct aatttttgta atttttcttc catctttcgt
     3961 aaatcattaa atactgtgag tagttcctca tagatagtat tttctaagtt aaattcgggt
     4021 atctgatcta aaagacctac acttacatca tttcttaaag ataagttacc ctcttgtaag
     4081 tcttcctgac cgttaataat cttaaaaatt gtagttttac cagacccatt tgcccctatt
     4141 agggccacct tttcaccttt gtttactgac atagaaaagt catttaaaac ttcttgggta
     4201 ccaaatgatt tagatataga atcaatattt actactacca taattatcct ccactaccag
     4261 tgtaatattg tatcgaactc caaactctct acaaattgat ttaatatttc ataatggttt
     4321 ccttctacaa gtatatgatg gtttccaatc ggattcttta aaaagtaact gatatcatcg
     4381 ccatcaaaat ctaataggac ctgggtccta caggcaaatt cactgtttaa attattaatt
     4441 aattgcgcat tttgaaatgc tatatgatct agtttttttc caccaagctt aaatactgta
     4501 atatttcctt ttttaatttc gccctgaatt ccgactccaa taccggtctc aaagtgagaa
     4561 cggataatat aatcatttac agacttagtg gccactgtac agtgtgcaaa cttaacctta
     4621 ttatttttta tatttaagga aaccggattt gccataaaag gaatattacc agttaaataa
     4681 taggttatca tcattgaaac tgttgctggt atatcaccct cacagcctgc cacaataccc
     4741 tcgttattca gtaaagaaag ggctaaacaa cctgtggttt ccaactcggt taccagatca
     4801 aaacagcgga tagttaaaga attaagatca tatctattta ctatattttt aagggcaaaa
     4861 tatactttag ccgctttcat cagatcattt tcagaaggct cttctatcga ggtggcttta
     4921 tccgtaaatt catttattag attttgagcc ctttcttcat gtatattatg gtattcatcc
     4981 agtacttcct ggatggggat ctcaataact tctaagcccc atctgtcttg tactgcctgt
     5041 ttatctatat gactatctat taaccaatca gaaggttctc cgataagtcc cagtcggcta
     5101 tttgctaact tatctttagt ttcctttatt ttgattaggt tctccatctc tttagctatt
     5161 tcggttggtt tcccgtgtag aatcttacct cttctatctt gcgattttac ccaacttaaa
     5221 atttccatcg aagcagccag tgaattgtgc aaagaagtgg ctactaaaat gacagggcct
     5281 tctaatttgt caaatatctc tttaaagata ttctctgtac caccggtttt gacgaaaaca
     5341 gttattaaat catactcttc ttttttatct gaacttaaaa tctcatcatt aatttcttca
     5401 acttcaatat tttttgaaag gtctttaaca aactgttcta aagtttgaga aattgcatct
     5461 tcatcatgaa taggggaaaa cagtgaatac attcctactt tcatctaaat catcctttca
     5521 aactcatttt tatcttatgg cagattatag acaggttctt ccatgtttct tctgaaaata
     5581 ataaaaacat taccaattac ctggaccagt tctgctccta attgatcaca tacctcatgt
     5641 gctacctcat taatatcttc taaagagtta tccaatactc taattttaac taactcatga
     5701 tcatctaata cttcatctat tttttcatag acggcttcat ttaatccttc tttgccaata
     5761 tggacatatg gatctagatt attggcttct cctcttaaaa aacttcgttg tttacctgtt
     5821 aacattacat ttcctcctat tccatatatt caaattcgat gggaccgatt ataacggtat
     5881 caccatcttc aataccttct tttttcatta tctcatctat attatgatgc ctgactattc
     5941 ttaataatct ctgaacagaa gcatcattat taaaatcagt tttttctatt aattcatcga
     6001 tcaatttacc aacaacttca tatttattat cagccagctg ttttaactgc atttcacttt
     6061 catccatgaa ttcaggtttt attaatacat cttcctttgc ttttatttct ttttcttttt
     6121 ctggcaagct ttctaacttt tgtccaatat aatatttaag tttatcaata ccttcacctg
     6181 ttacggctga aatgggaaat accttatatc ccctgtcttc taattctttt ttgacttctg
     6241 gtaggttttc tcgtgcttct tttaaatcta ttttatttaa agctacgatc tgaggaagtt
     6301 tagataaatc ggtatcatac tgttttaatt cctggtttat tttttcgaaa tcttcaatgg
     6361 ggtctctacc ctcaatacct gaaacatcta tgacatggat taatagtctt gtacgttcta
     6421 gatgttttaa aaattcatct cctaaaccaa cacctttatg ggctccttca attaaacccg
     6481 gcacatcagc catcacatat gatttatatt catcataaga aacgacacct aaattaggat
     6541 gaactgttgt aaagtgatag gaagctatct ccggtttagc agctgaaacg ttagaaatta
     6601 aggtggattt acctacattt ggataaccca ctagtcctac atcagctaat aattgcagtt
     6661 ccagtcttgc tttcacggtt tccccttttt caccattttc ggaaaatttt ggggccttac
     6721 gagtagattt tttaaaacgg gcattacctc taccaccttc accgccttct gctaatacaa
     6781 actcctgacc ttcttctttt aggtcggcta aaagttcgtt tgtatcatca ttatagacta
     6841 aagtgcccgg tgggactttt aaatagaggt cttttccatc tttacctctc tggtttttac
     6901 cgcgcccgtg ggtcccattt tcggctttat agaaattatt atatttaaaa tcagcaaggg
     6961 tatttaaacc ttcatctact tttagtatta catccccacc gtggccgcca tcaccaccgt
     7021 caggtccacc cattgcttca aatttttctc ttctaaaact tacaacacca ttacctccat
     7081 ccccgctttt tactttgaat gttacttcat ctacgaacat tatatcacct accttatttt
     7141 tagataaaaa taaaaccccc agcacaaaag tactgagggg tttgatctgc aaggatgatt
     7201 aacttattaa aacttccacc tgatcttctg tataaacgct aatttgacga gattttcttc
     7261 cttttctctc aaatttaaca taaccatcaa ctttagaaaa gagggtatca tctttacctc
     7321 ttcctacatt taatccaggt ttgaatttag ttcccctctg acggactaaa atactacctg
     7381 caggaacaaa ttcaccatca aattctttga ctccaagccg cttcgatatg ctatctcgac
     7441 cattcttaga acttccaaca cctttctttt gagacattaa aactcccccc tttcagtatg
     7501 ttgaattttt ttctctataa aaaattaagc ttcaatgttc tcaattaaaa cttttgtata
     7561 aggctgacga tgtccggttt tcttataata acgtctcttt ggcttatatt ttaaaacagt
     7621 aacttttttg tttttgccct gttctaaaac tttaccggaa acttcagcac cttctaaata
     7681 tggtttgccg aatttatatc cctcttgatc agaatactgt aagaccctat caaatgtaac
     7741 ctggtctcct tcttcgacat ccaatttttc aacttttatc atttgtcctt ctttaacttt
     7801 atactgtttt ccaccagttt taattattgc atacattact acattacacc tcccttacct
     7861 cagactcgcc ggaaattagg tagtctcaca tgagacattt cagacccact ccgtgcggtt
     7921 ttggtaatat acctctacaa gaggtacaca tcttaccgac aatttatttt agcatagttt
     7981 aggcttatag tcaagccaac cagagctttt ctcatttggt tttacagtta agagatgggc
     8041 agctttttta aaaccagccg catctaaatt atgggcatca gcagaacaag tctgcatttc
     8101 aagacagaaa aactttttat ttaacggagt ataaaactgg atatgagaaa attcttttga
     8161 ataatcaaat tcaattatta atttatcctc aaattttatt ttaggcttgt cttgcagctc
     8221 tgtgattgca ccatctatat ttatatcttt taaagaccta tagtcattaa aatcataatc
     8281 agtttcctct accccgagaa gtttaccagt ggggatttga tcagcatcca actccatata
     8341 agtttcagcc ggagctttta tataagaatt agccctttgt ttgagataat taaagtaagt
     8401 atgaaaacag atcccgtaag gtagttcttt ggaatcttta ttgtttactt catactttaa
     8461 tctcaattta ttattaaaaa gtttataaat gatttttaat tgatgttcaa aaggaaaacc
     8521 ttgatagacg ggatgatctt tatcgatata tataaaggtt tctaatttaa tatgatccgc
     8581 caagatctca ggttctttat agctccattt ttcatcatag actaaagaat gaagaaatat
     8641 tggtttactg tttttttcct gccaatattt cttgccttta aattcataat ggcagttgtt
     8701 tacccgattt ggtaaaggat aaataatggg gttaccattt agaaaatggt ctaaaggcag
     8761 gtcgggatca taatatatta aatccaggtc attatactga tacttaaaca tattattacc
     8821 aagaccaggg gctattttaa tcacattttt attattttga tcagctgcct taagggttac
     8881 tacccggaaa ttatcttcag aaatttcaaa atcaaaatga ctcatttcaa caccccattt
     8941 taaagcaatt ccgcctttgc aaaggttcta tggacttcat tgatttttac ttcaacctct
     9001 tcttctttca gatgtccagc attttttact ataatgatat aaccttttat tcttgctata
     9061 ccatcatttt cattatggat atgagtatct tctatatata tatcataggt ctcaccttta
     9121 tttaccggta aagccatcct ttttaagtcc tgtttgctac ctttgctgat tatttcataa
     9181 ttctcaatat gataatcatt attacctctt atataaatat ccatattgag ttcctcttcc
     9241 aattcagtca gcttatctcc tccggccccg attaaaacgg ccgctacatc gggatgaagt
     9301 tctattaata atgcttcaac cttttcctct ttagacagct tttttagttc tcttattaca
     9361 ttcattgcca tagttgtttc agacataacc aagccagtac cctcacagta agggcaatct
     9421 ttttgaaata attccccgat ctcttccctt acttttttcc tggtgatttc tactaaacct
     9481 aatttagtta ggcccagcag ggcagttttg gtgcggtctt tggccaactc ttcttctaat
     9541 acccttaaaa cctctttttg gttttcttca attgacatat caataaagtc aattattatg
     9601 ataccgccaa tatctcttaa cctcaattga cgggctattt cgcgggctgc ttctacattg
     9661 gttttaaaga ctgtatcctg taaattcttt ttgccggtga atttaccggt attgacatct
     9721 attgaaacca atgcttcggt attatcaatt attacataac cgccagaatt taaccatacc
     9781 ttccgctgcc agatttttgt gatttcattt tctatctgat aggtttcaaa aataggggtt
     9841 tctctctcat ataaatatat tttttggtgt gaaccaggta cacttgtttc agccagttca
     9901 tttaattttt catattcttc ttcatcatcg atcactattt tattaacatc aggactcaga
     9961 taatccctta caatctgttt aataagttca gcatctttat agattaattc aggtggtttt
    10021 gctcttttat agtctttcca aatattttcc cataagtgga ttaaaaaatt aaagtcattt
    10081 tcaatgattt ctctttcttt accttctgta ttggttctta caattaagcc atatttattt
    10141 ccaattaaat tttcagctat atctttgagc cgatttcttt ccttggtatc atcaattctt
    10201 cttgaaatac cagttctatt ttcaaaaggc attaatacaa gatagcgtcc cggtaaagtt
    10261 actttacagg ttaccttggc ccctttggtt ccgattggtt cttttaccac ctggaccatg
    10321 attttttgac cgggttgaag gacttccttt attgataatt catttttcct ccaccgtttt
    10381 tcctggtctt tatttaataa tggataaaca tcacttatat gcaaaaaagc atttttttca
    10441 agaccaatat caacaaaacc ggcctgcatt ccgggtaaaa catcttttac tctaccttta
    10501 taaatattat atactatttg atcataggta tctctttcaa acattatatc gatcagatta
    10561 ttattttctt tgatagccgc tctcttttct cttatacatg aatttattat tatctgtcta
    10621 ctcaattctc tctcacctct ttttttaaca cctgtctatt tataggttta tataacttat
    10681 ctttctttct aataaacatt cccaatcttt ctatgttaac caggggaatt tcttctactg
    10741 acccctcact ttttatcttt aaagctctta tcacttcctg gggtcgtaca tttccactac
    10801 tacctgtact gattgtaaat tcccagatgc catcttctac tacctgtcca gcatgaatca
    10861 tgggggctaa atcaattaat ctatcatctt tttttctgcg aaaacgtgta atttcaattt
    10921 ctttttcccc taaaaattct tttaaaattt ctttttgctg aacttctttt ttaaaggaca
    10981 tcttatatcg atatctagcc gtattaacaa tagcctgtaa ggacttctct ttttcatgga
    11041 tttcgatcat atctaaaacc cttaatcccc caggtagttt ttcattaatt tgtttcttca
    11101 ttaaatcaaa tcgaattttt tctttcaact cgagatcaaa atattcatga tagccgacca
    11161 tccccaccgg taaaggctga gacatggaaa atataatatg gggattatag ccttgtgaat
    11221 acgatactgg gatcttagcc cttctaaatg tccttctaaa agtattcatt aactctaggt
    11281 gggaaatata tttaagggcg tctaattttt catattcagc tcttatatac attgtaatca
    11341 ttccctatta tatctaagtc aacatctaaa ttaatacata tattacaacc tttacagtta
    11401 gcaaatcgac agtctgaggt ggtttcgttt tttagagctt ttttatgttc cctttttaaa
    11461 aacttgggag ctaatcccat atcaatatgg ctccagggca gtatttcatc cttatttcgc
    11521 cttctcaaat aaaaatcagg atctaaatta ttttctttaa aagcttccag ccagatatca
    11581 aagtcaaaaa agtctcccca gccatcaaaa cgggcacctt tttgataggc acttattaat
    11641 acctctgata gccttctatc tccgcgtgaa aatatacttt ccagcagaga aaggtcttct
    11701 tcgttccagc tgaagtttat accctttgta tttaatttag atcttaaaaa gtttatttta
    11761 tctttcagtt cttctttatc caccataggt tcccactgaa aaggagtgga ggccttggga
    11821 ataaaggtgg atacactaac attaattcta atcggtttca tcctcttttt gttgtctctt
    11881 ctaatctttt tccctttttt taaaacgttt ttaaccatct taactatacc ttctagatct
    11941 tcttttcttt cagttggaag cccaatcata aagtaaagct taacagttga ccaaccttcc
    12001 ttaaaagcag cctcgacagc ttcataaaga ttactttcag atacaccttt attaattaca
    12061 tcccggagtc tttttgtgcc agcttccggg gcaaaagtaa gtcccgtctt tcttaccttt
    12121 tgaacttcat tggccagctg cacggaaaat ttatcaactc taagtgaaga taaagagata
    12181 ctaatctttc tttcttcata tttttttgta agttccttta ctaactcttc tatctgggaa
    12241 tggtcgactg tacttaaaga tgttaaagaa atttcatcat aacctgtact gtctaaaata
    12301 ttatctattt gttttagtaa ggtctctttt tttctttctc ttacaggtct ataagtaatc
    12361 ccggctgaac aaaacctaca ccctttggta cagccccttg ctacttcgat agttgcccta
    12421 tcatgcacta tttccatata gggaacaagg aagtcttctg gataaaaagc ttcatctaaa
    12481 ttagatacta gctgtctttt tatcctgggc ttgattcctt tttgcttagg gtcaatattt
    12541 gttatatggc cttcttcatc ataactgact tcatataaag aagggacata aacaccgggt
    12601 ttatgagata attgttttaa aatttcttct tttttatacc cctgttcttt taaatcatca
    12661 tattcattaa taatatcaac tatatgttct tcagcttccc caataaaaag taggtcaaaa
    12721 aagggcgcta ccggttcaat attgcttact attgaacctc ccccgataac tagagggtcg
    12781 cttgccttac ggttcttact atagaaattt agtccagcca attctagaat tgttaatata
    12841 ttggtataac tcatttcata ctgtaatgta aaagccagga tatcgaactc atcaacactt
    12901 ctacccgact ctaaagaaaa taggggtaac tcttcttttc ttaaaagttc ttccatatca
    12961 ggccagggag aaaaagtcct ttcacaaata atattatctt ctttatttaa taaatggtat
    13021 attatcttta aacctatatg agacatccca atctcatagg catctggaaa tgcggttaat
    13081 acctttattt tatctgcatc ccactctttt tttattatat tatactcatt acctatatat
    13141 cgttctggat tttcaacttc aattaatcta tcatatattt gctctcttac attcatctac
    13201 aattactcct taaaatttta ctacgtcaaa aatgtagttt tttctttcta atatttacat
    13261 tcaaagctat gccaattgcc actattgaag tgaccatcga tgtacctcca taactgataa
    13321 aaggaagcgg aatacctgtt atcggcataa cacccattgt cattccaata ttttctacaa
    13381 catgaaagaa gaacatcgag attatcccag taaccactaa cttaccatat ctatctttgg
    13441 cctgaaaagc aatattaaat agctgccaga atagtagtaa aaagagtatt atcactataa
    13501 atactccgat aaagccaaat tcttccccaa tgacagagaa aataaaatct gtatgttttt
    13561 ccggtagaaa attaagctgg ttttgggtgc cggcaaatag gcccttacct gttaaacggc
    13621 cagatcccag agctatttta gactgaatta tattatatcc tatcccctgg gggtcgatac
    13681 taggatttac aaaagctatt agtcgattta actgatattc ttttaaaaac ggtaagggag
    13741 tgcccaaaaa tatgtgtgaa gctatcactc ctattgttcc aaaaaaacct cccccgaaaa
    13801 atacatacat aaacttttta tttgcaccag caacaaaaag cattactaca aagattacca
    13861 ataatactaa tgctgtccct aaatcatttt caaatactat gagcaaaaaa gggatcaaaa
    13921 aataaccgaa tggtaaaata aaatccttaa atgaatttaa tcctttcctc tgatctaaaa
    13981 tcgtggctaa tactaaaatt aaggcgatct ttgccaattc agaaggctga atactaataa
    14041 atccaaggtt tagccatctt ctcccacctg caatattggc tccgtataaa tgagtcataa
    14101 ataaaacaat aatcatggaa ccaaatatta attgggcata ctccttgaaa atacggtaat
    14161 caaaaaattg gattactata gctgtaataa cacccaatac agctgcaata atctgctttt
    14221 tcaaaaagat agagccgctt gaaccagttt tatttaattc cacagcacta cttatggcaa
    14281 acatcccgat tacaattaat aaaataaccg ttattggtat atataaattt aaatttttaa
    14341 ataacttttt ttcaaaatgc atagtttact tccttctatt gattattcct cctgagtgga
    14401 aagttagcaa ccattgccat catctgttct tctctattaa tttccatttc aatttcttga
    14461 tcatctacat caacatattt tttcataact ttaagtagat cttttttaag tgactccatt
    14521 tctgatggag ataacttaat gcgatcctga actaagataa attgaagtct ttcctttgca
    14581 acatttttac tggattcgtc ttttttcttt ttctttttga aacccaagaa ttctaataaa
    14641 ccgattgtca ttaccccctt atattaagaa aaactaaata tttttttaag ttttgtgaaa
    14701 aaggaattat cctcaagtga aatgaaatta acttcttctc ccgttattct acgcgctata
    14761 tttttaaaag cctgtccggc ttttggattt tcttttagaa caactggttc acctctatta
    14821 gaagaaacaa taataatatt gtcttcaggg acgatacctt ttaatttaac agcaagtatc
    14881 tcgataatat cttcaatatt catcatgtcc ccccgactga ccatatcagt atttattcta
    14941 ttaatgataa cttctgggtt tcttagccct tcagcttcta ataacccaat aattctatca
    15001 gcatctctta cagctgaaac ttccggtgta gtgacgataa gagcaaaatc cgcccctgca
    15061 atcgcattct taaatccctg ttcgataccg gcgggagaat caatcaccac ataatccatc
    15121 tcttttttaa ggtcttcaac cagagattcc atctgattgg gctgtactgc tgttttgtct
    15181 ctagtttgag ctgccggtaa taagaataaa tttttatgtc tcttgtctct aattaaagct
    15241 tgttctaatc tgcagttttc ttctacaaca tctacaatgt cataaactat tctgttttct
    15301 aaccccataa ctacgtctaa atttcgtaaa ccaatatctg catcaattag acatactttg
    15361 tagtccatca ttgctaatgc tgtaccaata ttagcaaccg ttgttgtctt accaacgcca
    15421 ccttttcccg aagtaattac taatgatttt cctgccaatt tattccctcc atttttttat
    15481 tatatttcaa aatcttcgac cataatttgt ccgtcctgaa taaaagccct ttctggtttt
    15541 aaactctttt tatttgaact ttcttcaggt ggtcttgcaa tttttttaga aattctaagt
    15601 tgagttggtt ctaattttaa agcagtgaca gaagcttctg tagaaccgct agcaccggcg
    15661 tgaaccacac ctctgatttt tccaaagaca ataatattac caccggcgat cacttctgca
    15721 ccgggattta tatcaccaat tattactaca ttccctgatt ttttaatctt ttggccagat
    15781 ctaagagttc tgttgattaa gtaagtttcc ccttcattat ttacttctac tgtatctctt
    15841 tttactacat cactggtgaa tataatgttt ttaacatctt tataacccct tgcaatattg
    15901 actatctcat ttaactgatc aatcttaaaa ctactttcag ctatatttat atataaatcg
    15961 gcccctgcgt aaaaattggt agcactgtta acatgatccc ttaaagcctc tttaatccta
    16021 ttaaaattag cttcgggatc aagagttagc actattccat ctttagcaac tttaaaagta
    16081 attggtgatt tcataattaa aatctttcct ttctcattat aataaattca ctagttaaag
    16141 ataaaatcct tttctaatct gaggaaaata catctcttat atattccaat aaattaccat
    16201 tttcctcttg ttctgtagtt tggttttgct ccgagttatc ttgttctgta tcttcgtctg
    16261 tggtctgctc ggtttgttct gattctccat tttcaacctc ggtttcaata ccaaaatact
    16321 cttttataat atcgccggct atggggagag tataagtact tgaactacca ttttctaaga
    16381 ataccaaaac agcaatttct ggattttccg cgggagcaaa accgccaaac caaccatggc
    16441 tgacatcctc tgtcccggtt tgggctgtac ctgttttccc ggccacattt atggggaagt
    16501 ctcgaaaagt actcctggct gtcccataag aagacatggt aacttcctgc ataccttcat
    16561 gaagaatctc gaataattta tctccgtagt ctaactttct aatcacctta gaattttgat
    16621 cgtaaactat atccccattt gcatctttaa ttttatctac taaaaagggt tggtataagg
    16681 tccccttatt tgcaatagct gcaatgtaat taacaagctg aataggggtg gttaataacc
    16741 caccctgtcc gattgataag tttaccgaat cacctgggta ccagttacca ctgttaccat
    16801 ctaaagcgcc ccgggggacg atcccttttt tctcttctgg taggtcaatt tcggtttttt
    16861 gtcccatccc ataatccaag gcagtactaa ctaatttttc cccctggtgt tcttcatata
    16921 acctatagcc taatttataa aatatgatat tattagaacg ggctatagcc tttgtaaagt
    16981 caagttcgcc ttccccaccg ggatgccagt ttttaaaggg tcttgaccag ccggggatgt
    17041 aaaataaacc gttttcatca acaaattctg tttctgcatt tacatttaaa aatctcactg
    17101 cagcgctgcc ggtgacaagt ttaaatagag aacccggagg cacagcagcc ataatatttc
    17161 tatttaaaag aggtttcata ggattatttg agagaacttg gtattcctga gaagttaacc
    17221 ctgtagaaaa acgattaggg ttaaaatcgg gataactggc catagccagc actttaccat
    17281 tattgggatt taatacaaca gctgatcctc ctgtcggttc ataccgttct ggatcctctt
    17341 ccgcctcttt cataagcctt tcaatatgtt cttttaagag tttttcaact tctttttgat
    17401 aatcaaggtc gaaattaaca acaatattat taccagctac cggaggttta gtaccaagtt
    17461 ccttgaccat ttcccccagg ttattaactt caatctgcct tataccatca acacctttaa
    17521 gatatgattc atactctttt tcaaggccgg atttaccaat catatcctgg acatggtaac
    17581 tgttaatccc ctggctttga tagtgtctta actctgcttt actaatttcc cctacatatc
    17641 caataatatg agcagctaaa tctttataaa catagtctct catagaagat tctttgacta
    17701 aaattcccgg taactcatct tgattttcca gtaatatcac cacatcttct ttatttatat
    17761 ttcttttcaa gatgagggcg ttttgattgt tcatcttctg tttactaaaa ttataattat
    17821 tttgcaacat ctcataggac attgaagtta aattggcaac actactcagt aatgaatcaa
    17881 cctttacttg cgggggaatt tcatttggaa gtaaatagag gttatgagac agcttgttgt
    17941 taactaatat atctccatca ctatctatta ttttaccacg gggggcacta atagggcgaa
    18001 aggaggtcct attcccttca gataagttat agtaataatc accatttaaa acttgaattt
    18061 gaaaggctct cgcgattaga agcacaaata ttattattaa tataatttta aaaactttta
    18121 ttctatcttc cataataatt ttcaccataa taaaatagtc tatataaaat aaaatacacg
    18181 atcaaagcca aaatggcgtt gtaaattgaa ctgggaataa tcacgcttct gaaattttcc
    18241 cagtaattga tactaaatat taattcttct gataaaacaa tcgtcaaaaa gtagttagca
    18301 atagaagcaa taaagacgat aaaaggagcc aataaataat tacctttaaa gaaatgacct
    18361 tctataaaac cacttataaa accaagtggg gccttaataa tggtattaac tgagataaat
    18421 tcacccagaa aaatatcctg cagtaatccg ccaataatcc ccattaacaa accggtctta
    18481 ggaccatcaa gtatagatat tacaactatc aggaccaaaa tcaaatccgg tttaatatcc
    18541 cagggagtat atagggataa aatagtaatc tgcaggacta atccaattat taagattaaa
    18601 acaaagatta tattgcgatt caaaaagagc cctcctaaaa atcagtgatg atcaatacct
    18661 cttcgatggt atttatagta ctaaacagct tgatatccgc actctgggat aagccataat
    18721 tatcagggtt tatttcttgt accttgccaa tgggtaaccc ttttggataa ttattggaga
    18781 acccggatgt tactataata tctccttttt gtatatcagc atcccaggag attttctcca
    18841 ttatattcat tttattatta tttaattggc cctttacaat gccgattgtc cttgattcat
    18901 ttctttgtac aattccacca actacaaatt ccggatcata tattaaagaa acctgggaac
    18961 tgctggaacc tacatagtct actcttccca ccagtgcacc attgtaacta attacaggca
    19021 tcttttttct gaccccatca ttagaaccgc gattaataat taacttattc tcccacaaag
    19081 aagggctaaa accaataaca gttgacccga gagtttcaaa ggaaacatgt tctttaaaag
    19141 atagaagttt tcttaatctc tcattttctc tttgaaaacc tttcagcata agattttgta
    19201 attttagaga agcaagttcc ctctctaacc ttttgttttt ttccatcact tcatctacat
    19261 tcaacagccc atgccaataa gaaactgttt catagtaaat tcccgaaaaa aattctatca
    19321 aaggactgac taaattatat atcccgcttt ctatatactg aaaatatgaa atatttatat
    19381 tgaaaaaata aagggttcct attaaggcta ataatactat taaaatcgcg gcaaatatca
    19441 atctaccttt attattcaat gtctattccc cctatgataa tttttttggg gtaattaaaa
    19501 gatccttgat ttcatctaat tcttctaaag cattaccggt accgttaact acacagtcca
    19561 gtggttcttc tgccagataa accggcatct gagtagtatc gatcaataat tcatcaatac
    19621 cgtgtaataa ggctcctcca cctgttagga ttattccacg tcccatcacg tcagaagcta
    19681 attcgggtgg agttttttct aaagttcttt taaccgcatc taaaatatta tttactggtt
    19741 ctttgagagc atcttttatt tcttctgaat taattgtaac agtttttgga aggccattaa
    19801 cgagatctct acccttgatc tccttggtta atcctccatt atcaccggta gcagttccta
    19861 tatcgatttt aatctgttcg gcagttcttt caccgaccat cagtttatac ttctttttaa
    19921 tatattgaga aatggcttca tccatttcat caccgccgat tctaattgaa ctacttgtta
    19981 caataccacc taaggatata acagcagttt cagtggttcc accaccgata tcaactatca
    20041 tgttcccggt gggttcatga atcggtaaac cggctccaat agcagcagcc atcggctctt
    20101 ctattaagaa cgcactcctt gcaccggctt ggagggcggc atcaataaca gctctctttt
    20161 ctacttctgt aacaccagaa ggaacacata ttataatacg aggtctaacc aacctatttc
    20221 ttttatgagc ttttctaata aaatgtctta acatggtctc tgttacatca aaatctgcaa
    20281 tgaccccatc tttcatcggt ctaactgctt ttatattacc gggagtccta ccaatcatat
    20341 tttttgcttc attaccaact gctaatatct ttttgtcttt ttcttttaaa gccacgacag
    20401 atggctcacg gatgataata cctttacctt ttacatatac caaagtatta gcagtcccta
    20461 gatctattcc catatcgcgg gaaaaaggtg cacttaaaaa attaaacaac tttattcctc
    20521 cttttatata tttcaaaata aattttttgc ttttaaacta atatattttc cgtctcctat
    20581 tattaaatgg tctaataaat caattcctaa aacttcccca gctgaaagta atcttttagt
    20641 aacctctata tcattatcac tgggtgtggg atcaccactg ggatggttat gtgctatgat
    20701 aagggaagca cagctttttt taatagcctt tttaaaaact tcacgggggt gaaccgtgga
    20761 actggcaagt cccccttttg agatcaaagt tttcgctata attttatttt ttacatctaa
    20821 aagtaaggca tatagatgct cctgtttaag gtgcctcatc atcggcatca agagtgaaac
    20881 agcctctttc ggtgaattta aaagtatttc cccttgataa ttacagctgc ttatcctttt
    20941 acttaactct attactgcac ataattgtgt actttttgcc ttcccaatac ccttatactg
    21001 cataaattcc tcgggagtaa aatcaataat tccttttagg cccttgctct tatttaaaag
    21061 attactggcc agatcaatcg cagtttccct ttggtatccg gttctaataa ttaaagccat
    21121 cagttcttca gtggtaagac tattactacc atattttatc atcttttctc ttggtcgttt
    21181 atctactggc atctctttaa tagtgtattt tgtgttggat tgaattggta aataagctcc
    21241 tttctatatt ttattattat aacctcaatt aaattatctc tatactaaag ctttttaagg
    21301 ccattgataa cagatgaatt ggtagtccca tcacagtaaa gtaagaacca actatcttat
    21361 ttacaaagat tcctccctta ccctggatac cgtaagaacc agctttatcc attggttctc
    21421 cggtctttat atatctttct atttcttctt tttctacttt tcgcataaat acttccgtta
    21481 catcataatc taaaaaatgc atctcatcat cagctgagat cagggccagg cctgtaaaaa
    21541 cttcatgttt atcattttct aataactgca gcatcctttt ggcatcttct tcatcttcgg
    21601 gtttacctaa gatcttgtta ttatgagcaa ctacagtatc agctgctatt attaaagcgt
    21661 tttctacaac attggccaca ttagaggcct tagccaaaga tagttcttgt actaatttat
    21721 caggatgctc ataatcatac ccatcttcat ctattttact gggcatggct gtatatttga
    21781 tccccaaacg ggtcaataac tcttttcttc tatctgacga agaagcaagt ataatctttt
    21841 tatctatcat taaacatcaa tcctttttag tccgcgtgga agataattgt aggtaaagat
    21901 tacaatcaat ccaattccta ccccagtagg taaagcaagt agtattaaat aaggcaggta
    21961 ataaaagata gcatcatttg atatgatgag ataagccgta attatttgtc ctacattatg
    22021 aaaaacggct ccccctaaac tcacaccaat tatactaaat ttatttctga aataataaaa
    22081 aaacaagctc attattaaat aacctaaaag tccgcccgtc aggctcaaat aaaagttgat
    22141 agtcataaaa gtaccggcca aaatagaacc caaaaaaatt cttagtaaaa gaatttgtag
    22201 gccggcaata aaaccaaata ttataagccc cagtagatta gcaatatttg ctaatcctaa
    22261 ttttgctccg ggtatgataa acgataacgg cagcatattt tcaactaaat gtaatactaa
    22321 acctaatcca actaataggg caataattac tatttttctt gtccgcattt aatttaaacc
    22381 tcacttcgac tttctacaga aaaatttaaa ggtttatttt aattatttca acaaaaaata
    22441 cttatttcct ttaatctgta taaatttata ctttaccagg aaaccgcatc aagctcacta
    22501 ttgctttttt ctatccagat cgaaacttta tttggtacac agatgatgga agggcccggt
    22561 ttagatatcc agcctgtttt ttcacagatc ttagccggat cttcctctgg cgcttctttg
    22621 aggcgaactc tcccattatg agcttcaatt acagaagtac ccagaggacc agtgacttct
    22681 ataattattg gttcttcttg gtaagtatca gaaaaaggta ttcgcgtaat ttcctgtgaa
    22741 ctatccttaa tatatatgta tgattctgta tcttcgttgt tattaaaata agcactgata
    22801 aaaggataag caatcattat aagggaaact acaattatta ttacaattaa tatcctgtca
    22861 taaaaagtta aatatttatt aattttttcc aattaaatcc cctctcctaa tcgttatcat
    22921 tttaacattt ttttatgaaa atgtctatct ttatagggaa ttaaaaaagt tacttgcaaa
    22981 gtaaaaaccg agcttattca gctcggcttt agtatattat taaaattata ttttctttta
    23041 atcttcatcc tcaacttcat ctttgtaaat aacattgata gcaccatcta ctttacagac
    23101 ttctacacat aaaccacatt gtacacattg gtcctgatca atctcatgaa cttcttttaa
    23161 ttctccttca atcgcatcga ccggacaagc ttttgcacat cttgtacagc ctacacagtc
    23221 ttctgtaata gtaacttttt ctatcaacct accattaaat tcaatggtcc cggtcggaca
    23281 tttctcagaa caaattccac agtttataca tttatcataa tcaataactg ctaggttatc
    23341 ttccatcgta atcgcatcga ccggacaggc cctcgcacag agtgaacagc caatacagcc
    23401 aacttcacaa atcttagata caactttgcc cggttcatgg gaagaacatc ttatgtgatt
    23461 taacttatct tcaggtgcca ttgttattat gtttttggga catgcttcta cacattttcc
    23521 acaacctata caggtttctt tatctataat gggtaaacca ttatcactca ttgtaattgc
    23581 atctaccgga caaactgctg cacaatcacc aaaacctaga caggcatatt gacagctttt
    23641 tgtaccaccg ttaatggatt cagctgcttt acaggtttta ataccctcat actcggagag
    23701 ttttttagtt tcttttatac ctccaccaca taaaagctgt gcttttacgg tttcattagt
    23761 attttctcct tcaactccca gtatctcggc aattttttca gcaactactt ctcccccgac
    23821 aggacaaccg ctgatctctg catctccttc aacaacggcc tctgcaaaac ttgcacaacc
    23881 tgcgtaacca caggcaccac agttagcacc cggcagaact tcttctactt cttcaattct
    23941 ttcatcgact tctacatgga atttcttgga agcaattcct aaacctaatg ctaaaactgc
    24001 tccaatacct cccatgctta atattgaata taaatagaca ggatccactt tgtcacctcc
    24061 aacaagtatg aattagattg aaattaagcc tgaaaaaccc ataaatgcca tagccattag
    24121 tccagctaat atcaaagtaa tgggcacacc ctgcagtgtt tccggtacat ctccaaattc
    24181 taatttttct cttaaacctg ccatcaaaac tattgccagg gtaaaaccaa tcccggcccc
    24241 taatccaaat actacacttg taataaagtt atatccattc aatggaatta aaagtgcaag
    24301 acctaaaatt gcacagttag tagtaattag tggtaaaaat ataccaaggg ctttatacaa
    24361 gaccggactt gattttctga taaacatctc tacaaattgt actaaagaag cgatcactat
    24421 tataaaagat acatatctta aaaacggaag attgaaagct tccagtatat atgtattaat
    24481 caaccaggta gcggccgctg ttaaggtcat gacaaaagtt gtggccattc ccatactaaa
    24541 ggcagtctct acttgttttg aaactcctag aaaggggcat atacctaaaa actttactaa
    24601 aataaagtta tttatcaata cagtactaat aaataccagt actacaactc ttagttcttc
    24661 cattaattac ccctcctttt aattgcattc ataatcccaa ctaatattcc taaactaata
    24721 aaagcaccgg ctggtaagat cataattacc atcggttgat accaactgcc tagaatttct
    24781 ataccaaaga tagaacccat accaaagaat tctctcatag cgcttaaaac caccaatacc
    24841 caggtaaaac cgattcccat acctaaggca tcggcaatag cccgatgtgt cgggttttta
    24901 gatgcaaaag cttcctgccg tcctaatatc aaacagttaa caactattaa tggtataaac
    24961 aaactcaagg aagctgcaat atcaggaaaa aatgctttta agaaataatc agtaaaagtt
    25021 acaaaggtag caattattaa aataaaactg ggaattctca cgttgtttgg tatataattt
    25081 ttaaataatg aaattacaat ctcagaagac aaaagaacaa atgaggtagc aattcccatt
    25141 gccaaaccat tttcaactgt atttgtaacg gctaatgtgg gacacatacc gatcactaat
    25201 actataattg ggttttctcg ccaaagacca tttttaaaat ccttaaataa tcttgttagc
    25261 attaagcccc acctccatat gcatcttgta tttgtttaac agcattttcc acaattttag
    25321 ttacactgcg ggatgatatt gtcgcacccg cgatcgcctc aacttcaaga tctgtttgcg
    25381 tatctctttt tacaaccgta taatcaccaa agggcttatc tacaaagttg cttttatact
    25441 cttctcccgt aatcctagcg cctaaaccgg gagtttcttc atggcttaaa atttttatac
    25501 catagatctt ctcttcattt atatttacac ctatcattat ttcaataaca ttctgatagc
    25561 ctggtcctga agcaatcatc gctactccta cacgattacc gcctgaatca aaaccttcat
    25621 aaaaagtatt cccttttttt tctacttctt caaactcttc ggcctctggt attactgctc
    25681 ttatagccct ttgtctggct tcagcttgat gttgttgtat atggggatta gtccattgat
    25741 agacaaaaga taaagcaaca gcagaaataa taccgataac agtcaacgta agtactagtt
    25801 tactgatttt agtatccatt acttcaccac tcccagggtc ctaggtctta aatatctatc
    25861 cagtaaagga acagtggtat tcataaatag aattgaatac attacacctt ctggataacc
    25921 accccatagt ctaataatcg caacaagcag accggctcca attccaaaaa cccatcttgc
    25981 tctcttggta acaggtgaag taaccatatc agtagccata taaaaagcac ccaggataag
    26041 actaccagca aaaagatgct caaccgggtt tgctcctgct aagaaagtca taataataac
    26101 agcagaaatc ataccggttg gtattctcca gtttatgtat cctttatata ttaagtaggc
    26161 tgcccctaat aataaggcaa gagaggaagt ttctcctaaa gaacctcctg tactaccaac
    26221 aaaaagattc cagattctta ctatttcccc gccagccatt tggcttaagg gagtagcggt
    26281 ggaagttcca tcaaccgtcc aggttgtcat aagtactgga taagaagcag ttaaaaaagc
    26341 ccttcctatt aaagccggat taaagacatt ataaccgagg cctccaaata cctgtttacc
    26401 cagacctata cctactacac tacccaaaaa ggcagtccat aatgggattg agggaggcag
    26461 ggttaatgct aaaagtaatc cggttaaaac tgcacttcca tcgttaatta caatttcttt
    26521 ttttctaact tttaaaaata tatactcagt taatacagcc cctaaaatgc ttacagccac
    26581 aattaataga actctaaatc cgaaaaaata tgtagcggct aaaacagcag gaataagagc
    26641 atatacaact gtccacatta tctcttcaac tgaggttgca tctttaatat gtggatttgg
    26701 tgttacaagc agttgttcat tattcataac tatcctcctt actgtgatga ttttttcaat
    26761 ttcatgtctt ccattttccc aattctaatg taatctaata gtggtctatc tgctggacat
    26821 atatatgaac aggagccaca ttcaatacaa ctttgtacat tatattcatc tagttcatca
    26881 tatctttcat actgtgataa atcagatagt ttaagtggta ataaatacat gggacaggaa
    26941 tcgacacatc ttgcacaacc aatacatggt ttaggatcga acttagccac actatcttta
    27001 tgtaatacta taattccaga agtacctttt acagtaggaa cttcagtcga atgctgcgct
    27061 gaaccggtca ttggaccacc caaaataatt ttggcagggt ctccttcaaa tcccccggct
    27121 tcttctatca gatctttaat acgagtccca atccgatagt aaagattaca gggttccatg
    27181 ataccctctc cggtcaccgt aatagtcctt tcaattaagg gtttcccctc attaaccgct
    27241 tcaaatacgg ctacagctgt ggcaacattg tttacaatac agcctacatc taaaggtaaa
    27301 ccaccggcag gtacttctct acccagagct gctttaatca gcattttttc tccaccctgc
    27361 gggtactttg ttttaaatac ttctatatca atattgtctt cctcttcaac cgcttttttc
    27421 atattcgata ttgctctcgg cttattattt tcaattccga tgaaaccttt ctcaactccg
    27481 gctgccttca tcataagctt cataccttct actatatctg atgaacgctc aatcatcatc
    27541 ctattgtcta catttaaaaa aggctcacat tcaataccat ttaatatgaa atattctact
    27601 tctttatcat ccggcaccgc tagcttaaca tgggtgggaa acatagctcc cccaaggccc
    27661 actatgccgg cctccctgac aacatgtcta atatcttctg atgttaaatc ttcaatagcc
    27721 ttatcgggtt ttaaagagtc atcaacttca tcttcttcat ctgcttcaat gataattgat
    27781 aaggtctttt gaccggaagg tgttcttaca ttttcaatcg ccttaacttc acctgaaaca
    27841 gaagcatgta caggtgctga aacaaatcct tccgcatctc caatcttttg tcccctttta
    27901 acatggtcac ccttttctac taaaggttct aaaggggcac caatatgttg ttgtaaaggt
    27961 attactactt cctggggtct tttagcatcc cgtaatggtt tttctttagt taattcttta
    28021 tgatattcag gatgaatccc ctgtttgaat gtaagtacac tcattttttc acctctaatt
    28081 tagtatatat ttgcttgtaa ataaacaact acgttaattc tataatatgt aagtaaaatc
    28141 tgcaaattat tttacaataa aagtatcata tcctaattca attaattctt cttcaatctg
    28201 ctccgtcgct tctctcgatt cctcacctat aactctgact ttgaaagtag acgcctcttt
    28261 tattataaca ttatatccct cgtcttcaag ttccttttta aaactttccg catttgacct
    28321 ttgtgaaaaa gcacctacct ggatcttata tacattacta taattaccac tggatacctc
    28381 ttctgtttgt tcggtttgtg ctgtttctga agtattatct tctgtagcct cttgttgttg
    28441 ggattcagtc tccggttctg actgttctgt agtctggtct acttcattgg aactttcttc
    28501 ttgctgggcc tcctgttgag actcgttttc ggtttgggct acttgttcag ggccaccttt
    28561 catccatcta aatagattta accccactaa gtatccaaca aaaacacata tgattgccat
    28621 gacaataatt acaagtgtta aagacctatt attagatttt ttctttttag ccatccttta
    28681 aacctccctc tattaacagg tgagtaaaaa atcttgcttc tgctacggta tataaagaac
    28741 cagttataca aaatgtatca tcttcatcca ttgttttata tgtctgcttc acagcttttt
    28801 gaatattatt ataaacttta tttttgagat tgagagtatc agcataattt tttaataaag
    28861 tcggttttaa agacctttca ttttgatttt tagtaattga tagttccatt ttatgatcta
    28921 atgtattaat tatattaaac atctctttgt agtctttatc ttttaatatg ctaattaaaa
    28981 agaataactt cttatccttc tttatattat catctataaa ttgtactaat cttttcatac
    29041 catctacatt atgagccccg tctaaaataa tattggggtt tctattcatc atttctaaac
    29101 gaccgggcca tctacaccgg tctaaacctt tataaatttt ttctttatta attttatatc
    29161 tagctggaag taaatttaaa gatcttactg ccaaagcagc atttttgact tggtgttccc
    29221 ccaatatatt aagttttata tcatcaaatg tttccccatt atatcttaaa ttaaatgtct
    29281 gctgttttaa attcttatca attaattcat aattggccgc cttttccatt atagttagtt
    29341 tggctttcat ttgggcagca atttttttaa aaacatccaa tgcttcctta ttttcagctc
    29401 ccgtgataac aggaactccc ttttttatta tacctgcttt ttcaaaggca atatctttaa
    29461 tctcaggccc taaaatatca gcgtgttcat aacttatatt tgttatgata cttaagaggg
    29521 gactgctaac agcattggta gcatccaatc tcccccccaa acctacctct aaaattccaa
    29581 tatctatctc ttttttataa aaatattgaa aggctatcac tgtaattatc tcaaaaaagc
    29641 tcggctcttc aatttttata tctttagctg gatcagcaaa gatatctttt aattcagaga
    29701 ctatctcttt tagttcattt gttgttatgg gaatctcatt aactttaaat ctttcattaa
    29761 agtggaccaa cggtggtgag atataagtac cgaccttaaa gcccgcttct ttgaagatac
    29821 tggttaaaat agcagctgtt gaaccctttc cattcgaacc tccaatatgg atgtaattta
    29881 ctttatcttg cgggttatct aaataagaaa gaagtttatt aactctttct aagcccggtt
    29941 gaaatccacc ttttttgcca tactttttaa attgtaataa aaactcataa ggattcatat
    30001 taccattcct tttcaacatt actttttaaa aactccttga agcttagtat acatttatta
    30061 taaaataaac tataacaaaa aggaggttaa ccgggtgaat aaaatactaa tagtctcatt
    30121 aatatcttta tttttagcac agtttttaaa aatatttact attaaaccct cttcttttac
    30181 caacttattt ggttcaggtg gtttcccaag ttcccactcc tcttttgtgg ccactctctc
    30241 taccctaata ggtctaaaac acggctttaa ttctgatctt tttgccattg tcttaatttt
    30301 ttctttaata gtcacttatg acgcgagtgg agtaagaaga gctgtcggag aacaggccaa
    30361 tgtattaaat aatttaatcc aacacttaaa tgtaaaaaat attgaacgcg atccggaaat
    30421 actaaaagaa gaccttaaag agttaatcgg acatactcct ttagaagttt ttgcaggtgt
    30481 gctattgggg gtttcagttg cttttataag ttattattac tttttttaat agttcttttt
    30541 tatataatct aatagctgtt ctcttttttt aaaaactccc aaggcctgtt ttccttctat
    30601 atcttctaaa atctctccca ctaatttacc ttctgcaata tttaatacct ttttaatatc
    30661 attaccactt atcaacttct cagcagttct tttctgcatt tctgctacct tatttaaaac
    30721 tttaaaaacc aattcttttt cttttttgtc tttttttaca tgaaaaaggc ttaatagtag
    30781 atattttgaa actttaatat ctatactttt tgagaacaat tcttcataca ataatatatc
    30841 attattaaga taagatttat agtctcttag taaatttttt ataaaatgaa gatgatctat
    30901 tactagatct atgccctgtt tattaaaagt atagttaatt aatctatctt ccagttgttt
    30961 taaagtaact ttatttttta aaatcaaaaa catgaaaaat aggataaggt taaataaata
    31021 actctctatt tttatatttt ctaagtatct atttttttta acaaaatgta gctgttttaa
    31081 taattttttt cttattttgc gatctttttt taaatctacc ccaaagagga ctttcaaaat
    31141 attactttct aatagtaatt taatcctggc aggctttaaa tagtttgaat acaattgata
    31201 tagttcctct tttattctct cattggctgg ttttaatgtt atattaatat tttcttttat
    31261 tatttcataa gttttttctt ctattagaaa gtctaaatta ttggaaaatc taaaagccct
    31321 taaaatccgc agggggtctt cctttataga atcctcgctt accatcctaa ttatcttatt
    31381 ttcaagatca cttcttccct caaaaggatc gattatatcc ttactatcca taaaaatttt
    31441 ctcattttta taacttacat ctcttaaatc taatgccatc gaattaatag taaaatccct
    31501 gcagccaaga tcttgataaa gatcctgacc tacaggggtt gtaaaatcaa taaatttgtt
    31561 atcatcagtt acaattctat agatactgcg ttttttatct aaaacaatca acttgcgatc
    31621 tgtttcatta gtaaaccgat ctataatatt ttcaaaatct gacataacaa ttaaatcaat
    31681 atcttttatt tctttattta ataaatagtc tcttactgct ccacccacta tatatatttt
    31741 gttaactcct gtaatatatt ttagtaaaga aagatatttc attaattttc acctatctta
    31801 atcaattaat ctttttatta ttttatctgc atcaaatcca aagtgggctg ctacttcctt
    31861 gccaggcgca cttgctccaa actcttcttg gctgatcacg gtatcatctt gatctaagaa
    31921 tctataccag ccgctgccaa tacctgcttc tataacaact tttttatcct taggattacc
    31981 cattaattct ttaatatagg ctctatcttg tgattctaag atactgcgtt ctggcacaga
    32041 gactatcctg gattcaatat ctttctcttt taatttatct gcaatctttt gggcaagaga
    32101 gacttcactg ccgcttgcta taaaggtaaa cctataatct cccttacctt ttctaactaa
    32161 ataaccgcct ttttcaaagt ttttaatacc attttctttg gctatttcag gtaagctctg
    32221 tcttgttaag accagggcag tcggtccttc ggttcttttt actgccttaa gccaggcttc
    32281 ttttgtttcc tcagcatcag ccgggcgata aacctgaaga tgcgggatcg ctcttaacgc
    32341 ttccagttgt tctaccggtt gatgagtcgg accatcttcc cctatataga tggaatcatg
    32401 ggtaaagacg taaataacgg gttgtttcat taaagctgca agcctaatag ccggcctcat
    32461 atagtctgaa aatacgagga atgtggcagc aaatggtctt acacccttat ggagtgccat
    32521 tccatttact actccaccca tggcatgttc ccttacgccg aaacgaaaat tacggccgga
    32581 atgagaatta ggttgaaact cttcatactt atctagataa gtcttattag agggagctaa
    32641 atcagctgaa cctccaatta aataatccaa tctatcggcg attgttctta aagtagctcc
    32701 agaagcttta cgggtggcaa tgggtgtatc aatatctaaa ttatgaacta tatcttccag
    32761 gtcttcttta acttctagat tataagcttt cttccattct ttatagagtt tgggatactt
    32821 ttctttccac tcagcaaatt tataatccca ggcttctttt ctttcctcta aaatctcttt
    32881 tctctcagaa aaatagtctt tcacattatc tgagacatag aattccttat cagttggtag
    32941 accaaaaaat tcttttaaac ctttaatatc ttcttcccct aaaggtgctc catgggcttc
    33001 ggctgaatcc tgtttattag gggccccata agcaatatgg gttttggctg caatcaaagt
    33061 aggcttagtc ggttcagctt ttgctttcat taaagccttt ctcatttcat ttatatcatg
    33121 tccattgaca tcttcgatta catgccagtt ataggcctta aacctgtccg ggactgattc
    33181 cgtaaatgtg atatcagtcg acccttctat tgaaatatcg ttatcatcat agattgcaat
    33241 taatttacct aagccaagat gaccggccag ggaagcagct tctgaactaa taccttccat
    33301 catacagccg tctcccatta tggtataagt ataatggtca actatatcta aatcaccttt
    33361 attaaattta gatgctaaat gcctttctgc tatagccatc ccgactgcat tggcaaaacc
    33421 ctgacctaaa ggtccggttg tagtttctac accctcggtt tctgagtatt cggggtgacc
    33481 gggggtcttt gattccaact gtctaaaatt ctttaactct tctaaactca aatcataacc
    33541 acttaagtgt aaaagagaat ataacaacat tgaaccatga ccagccgaca aaacaaaacg
    33601 atccctatca ggccaatttg gacttgcagg atcgtgtttt aaaacttcac cataaagtaa
    33661 tgatcctatt tcagcacaac cgaccggcat accggggtgt cctgaatttg ctttctcaac
    33721 tgcatcaaca gacaaacctc ttactatatt ggcaacttct ttaagcacat taaaacctcc
    33781 tgttgtatta gtctattaat tattataata ttcagagggt taaatttcat agcttttacc
    33841 ggtaatcggt aaatttaaat ttgctaaaac cctttctatt aaaacaccct ctctttctgg
    33901 aaaatcatag ccataagtgg tcctcacggc cattgaaata aactgtacag ctctatcaat
    33961 agccataggc aagctgtctc ccgaaagtat actaccaacc aatatactgg tgaataaatc
    34021 accggtgcct ggaaagttag ccgggatata atcacagtct actttccagt aacgatcttc
    34081 ctttttttga tacgccaata ccgaagtttt ttctttactt ttataacctg gtacactggt
    34141 gataactacc atatctggtc caaaagaaga aagatctctt aaccattctt ttgctttttc
    34201 atcgctgttt ataccgttct tatctttttt tagtaaaaac tttgcttccg taatattagg
    34261 ggtaataagg tccgcttttt tgactaattt tctcatgtta ttgaccattt ttggaccaaa
    34321 agtgtcatac gtctctccat tatcccctaa tactgggtct ataataacat attgttcgtc
    34381 tcttttaaat cttttaatga aatcaataac aatttctatc tggtgaacag aacctaaaaa
    34441 accactgtat atcgcatcaa aatctatagc tagagattcc cagtggttta tataatcggg
    34501 taattcttcc gttaaatcaa taaatttata gtctttaaag ccaccggtat gagttgacaa
    34561 gacagcggtg ggaagtgggc agggttgaat tcccattgta gccagtactt caattactgt
    34621 ggttaatgaa cctctcccat accctgatat atcatgtata gccgctactt tcgggactgg
    34681 atttttcatc ttcttcctcc ttactaacct atcgtactac cactttctat atttttatta
    34741 tcaaagtctt tttcccttgt ataagacata actctacagt ttcggccgat ctgaacatta
    34801 ttagggataa cggctctttt agcaatgata tttaatccat tgaagagtaa atcaggcttt
    34861 tcttcatttg gtgttaaatc atcaccaaat ccaatatggc agttctctcc tatatcaact
    34921 tctttatcaa taatacattt atcaactatt gtgttttttc taatccgtgt gtcattaaag
    34981 atgattgaat ttcttactac tacattttct tcaatataga caccggggga tattacagaa
    35041 ttctctacaa taccgttgat aatagcaccg tttgataaga ggctattttt tgatttgcta
    35101 ttggggccga actttacagg tggtttttcg acactttttg tatggacctt ccagtcttca
    35161 tcataaagat ttaattctgg aatagattcg gtcaaagata aattggtctc ccaaaaggac
    35221 tttaaagtcc ccacatctct ccaataacct tcatactcat aggcataaac ctcattattt
    35281 ttaatcatcg gtggaataac atggtggcca aaatcagact tttcagcact acagtattta
    35341 ttaagagcct taattaaagc atctttagta aaaacataga tccccatgga agccaagttg
    35401 tttgggggat tttcgggctt ttctaaaaaa tcagtgatct taccagtctt atcggtatct
    35461 aaaataccga acctactggc atcttcctgg ggtactcttt gggcagcaat cgataaatcc
    35521 gcattacgat ctatatggtc ttctaccatt ttatcataat tcatcttgta taaatgatct
    35581 cctgataata ttaaaacata ctcggggtct tgactcctta aataactcat attttgatat
    35641 atagcatggg cggtcccttt ataccagcca ccttctttac cggtataagg ttgaaggata
    35701 gtaatacctc catatttacg gtctaagtcc catggtttac caattccgat atgatcattg
    35761 agagaaagcg gtaaatactg agtaaggact cctacattat atatacccga atttacacag
    35821 ttacttaata caaaatcaat tagtcgatac ttgcctgcaa aagggacact cggttttgcc
    35881 ctatgttcag atagtatgtc taatctactg ccccttccac ctgcaagtat taaagcaagt
    35941 gttttcaatt taatcccccc tggattgtgt tatataaatg ttatttactt tttactcctt
    36001 ataattttat attttctaca ataaaccctt aaatccttta ttcaataaac taaaatatag
    36061 ttagcataac ttaataaaat taattaaaat tattaaatat ataaatattt atgtttaaat
    36121 ttttaataat tttcttgaca atattaaatt ttttaagtat aattattata gaaattaatt
    36181 aatggaggta taaaagtgga aaaaactaat ttaaaacttt taaaaaacaa agatttcata
    36241 ttattatttt taggaggttt tatttcccgc ataggtaatg gaattcatta tattgcccta
    36301 gtctggtttg tattagatat aacaggcagt ggttctgcta ccggcctgat attactttta
    36361 tctactttac ccggtgtaat aattggacca tttagcggag ttatcgctga taaatttgat
    36421 agaaaaaaat taatagtata tatggatata ctgagaggtc tcgtggtact ctggcttggt
    36481 tacaccgttt acataggaac agctaacttt ttccacatcg gtttagcaac agttttaata
    36541 gcagtctgta cctccttttt taatccagct gttaccgctt caattcctaa tatcgtggat
    36601 gacgagttat tacaaaaagc taattcgctt aaccatttca gcattaattt tactcagata
    36661 attggagctg cagtgggtgg tatattaatt gcaatctttg gaattgccgg tgtctttttt
    36721 ataaatggta tttcattttt gctttcagct ttctcagaaa tctttattaa tattccggaa
    36781 gtaaaaattg aagaagaaat agaaaaagcc cctacaatta taaatgaaat caagtttgga
    36841 tttaacttct tgtggcagaa aaaggaactt gtatcacttt ttacagtagc attattttta
    36901 aatttcatct caaccgggtt gatagttcta ggtctaccct ttgtttataa agaactattg
    36961 ggagtaaaca gtcaactata tggatatgcc caatctatct tcccggccgg tgctgttatt
    37021 ggttcaataa ttttaagtca gatccctgaa attagaaatt attttaaggt actcttctat
    37081 tccctcagct cgcagagcat gctgatgata atagtagcgg ttcctctatt accatacttt
    37141 ttagccaacg catctgtaat gacaatttat tattctttaa tgttaatctt atttgttgtt
    37201 ggaattttaa atgcagtagt caatgtaccg ataagtatct tgttacaaag attaataccg
    37261 gacaatttaa ggggaagaat tactggacta ctctcaacca tgtcacaggc tttagtaccc
    37321 atttcattgg ccctaacagg ctacttactt gatctagtgc ctgtttatac attattttta
    37381 ggagcgggta tagcttcttt aggtctctcc ttgtatatcg ctaaaatacc cgctatgcag
    37441 aaattaaatt acaaagagga aaaggtggag cccaatatgg aggtttctac aaatacactt
    37501 gatgcataaa aacattctgc atgaagaata gggcaatgta ggcaattatt aaggccatta
    37561 agggagttaa gatccagcct aaccctattt tacccaacag cttataattt acgtttctcc
    37621 ctccttttgc cagtccaata cccatcacag ctccgacaac agcttgtgac cctgatacag
    37681 gcacaagggg taggggcggc aatccaaggg ataataagat agatctcaga ccctctgagg
    37741 caaaaatgaa taagacggta gaacttgaca gaactactat cagtgaggcc accggggata
    37801 ttttaaaaat actcttccca acggtggtca ttactctttt agaataggta aatacaccga
    37861 ccgaaataga gatcccccct aataggaata aaacctgagt atcagaaagg gtaataaaac
    37921 ctatttttat tccttctaag gttgaggttt gtgtaaaaac tcccatcaca ttcgctatat
    37981 tattagcgcc taaactgtag gccccaaagg ctcctaccaa tattaatccc aatctggtat
    38041 acatatctat tttaaataaa tgcaagctat aattattaac aatatattga gctaatttaa
    38101 aaaacagcat cgcaaacagg gccgataaaa tcggagaaaa aacccaggta ccaacaattt
    38161 tgcttaaaac accaagttca gtactataac cactaaatat attccaacca ataatagctc
    38221 ccactattga ttgggtggta gaaacaggca gttcatagtt cgtcattaaa taaacggtta
    38281 aaccagcagc aagagcaact gtgaaggcgc ccggcattgt atttactgcc cctaatgttc
    38341 ccagggtttc agtagttcca gacccactta ccactgcccc gataataaca aaaacactga
    38401 taataatagc cgcggtggta aaacggacca tcttggtaga aacagctgta ccaaataaat
    38461 ttgccgcatc gtttgctcct aacgaccagc ctagaaataa tccactggaa ataaataata
    38521 aagataccat atgtctagat cctccctgct cttacagtat ttttatatag atctttttat
    38581 tgcagctaca gaaagttttt cactaatttc ttctgctaga tcagaaaaat aagcaatcct
    38641 ttcggaaaaa tgacgcaagt gtactttttt acagaagtca tctatctcat cagtgttaaa
    38701 gactttcctt tttatccgtt cctctatctt gtcggcttca tgttcataaa agtgaacctt
    38761 atttacataa tcattcacca tagaaatttc ttcaaaataa gtccttaccc ctttaaccag
    38821 ttcttccgaa gaattaagag attgtttggc cagatcttta aattctgcta aaacaaattc
    38881 tctaatctca ggtttttcaa tcgaaaactg cattaagact ttcttgcaga ggtcaacctc
    38941 attatccata tcttccaaca atgataagac atcgccccgt gcttccggaa tcagcatata
    39001 ggtatagagt ttatacttga tatcttttaa gatatcatca gctctattct ctatacctaa
    39061 aatatcttct aaattctctt caaaaaggtc ataatgacca tcaaaataac ggttaatatc
    39121 tctttcaaaa atgagtaaag cttcttcaac tgtgtccaga tattcatcaa tatctttttg
    39181 taattctttg gacttattaa aaacaaactt tttcaatttt gcttaccccc tttttgcttt
    39241 tacctttcta ttaaaaatgt tttgaggcat attgataatc acaattccta aagaaaccag
    39301 gatcaaagcc aggacagcat taatatttat tgtctctgaa ataataaaga aggccgataa
    39361 aaacacacca ctgaccggga caataaattt atatattgta atatatccca ccttgttaaa
    39421 tttaatcagg gtataccaca aaccaaaggc agcggctgaa atgaaagcca gataaattag
    39481 aagcatccct gattttagat caaaattaat tgatcttaaa tccgccccgc ttaaagctat
    39541 caaaaataaa gtaactgaac caaataacat ttgataggcc gtaataatca tgatatctat
    39601 ctcatcagaa agatacttga cataaaatat ggcaatagtg ctcattaccc cggacatgat
    39661 aataaaacct tcacctgtaa agttaaagac aaagtttaaa tttcctctgc ttaaattgat
    39721 gataatcaca cctgtaattc ccaaaagaag tccgaaagtc ttaaccttat tcaacttgtc
    39781 atcaatataa ataaagtgag aagcaattac cgtaaaaaat ataccggaag tcactaatat
    39841 agaagcctta actcccgtgg tattagccag gccattatag aaaaaagcat actgggcaaa
    39901 ggtttggatc aaacctaaag aaattaaatg tatatagttt gctttattca atttaagtcg
    39961 tctaatgcct gatttgataa ctataaataa gatcaacata aaagaggcca gcataaatct
    40021 ataggaagca aataacatct tattaaaagg accgctgttt tctaattcta gtatagagta
    40081 acttaccttt aaagagggaa aagcgctccc ccatagtata caggcagcaa ttgctaatag
    40141 tacaacagaa tatttatttg taaatatatt gaatttttta ttatccatct tatgtttttc
    40201 cacctgcaat cttttctttt tgcttttgat tagaattaat ggctctaaat ataccgtata
    40261 aaattagtac tccccctatc atggtagccg tcgttaatct ttcacctaga atgatcattg
    40321 ctaacactgt ggtcataatc ggttctccca ggatagctaa agatacgatg gtagcaggta
    40381 catatcttat tgacagattt aaaaccgaat gtccaataag ggtcggcccg agagctaaaa
    40441 ttaatatata gatataattc atattaccat agccggtaaa agaattacct gagagtaaga
    40501 caaatataaa taagaagaag gccgtatagg aatatagaat atagatatat ggtatatact
    40561 taatctcttt tctaaaactc ctacctaaaa ataggtaaca ggccgctaaa aatgctgcca
    40621 aaattgccaa catatctcca tagaaccggg ttaatattat ataaagatca gaaaaactaa
    40681 taattatgct cccgatcagg gcgaatatca caccggcaac tgcccctttg cggacatctt
    40741 ctttggcaaa taagtattct aagagtaatg taaataatgg ctggattgca ataaagatta
    40801 cggcattggc cacgtttgtc aggtgaacgg ccgagatcca ggcataaaaa tgtaaggcca
    40861 atacaaaacc aactaaagcc ggccttatat caagaaaaaa tttcaaagag tccctgtatt
    40921 tagtaaagaa tacgggcgtt aaaaatattg aagttaagaa tactctataa aaagcaatta
    40981 ttaagggtgg agcagtggta actttaataa ttatacctga aaaagaaatt ataaagactc
    41041 caatagccag tattatatat atttttatat cattttctgc taatttcata atatttagct
    41101 cctaatttag taagatatgg tcattatcct tatctaattt aatttctctg tccttgtgtt
    41161 taaactgaat agagtcacct tctaataagt tatattcaat ttcatcttct tttacgtgca
    41221 ctttcagcaa ggctccccta tactttattt taaattcata accatcccat tgtttaggta
    41281 aatacggttt aaaaattaat tcaccttcat aagtcctcat cccggcaaaa ccctgtacta
    41341 ataccatcca attactgccc atacaggctg tgtgtagtcc ctgataggca ttttcattat
    41401 agtcatctag atccaatctt gccgtttgtt taaagtagtc ataagcatcg tcatggtaac
    41461 ctatttcaga ggcaataata ctgtatatag aaggagataa ggaggaatca tgtgttgtct
    41521 ttggctcgta ataatcatag tttcttttct tttgttctaa actaaactta tctcccataa
    41581 aaagcataag caggatgaca tcagcctgtt tgagaatctg gtacttccaa atagttaaag
    41641 gatgccagtt tttaactaaa ggcagttcat caatggggat cgattcaata tcaatttctt
    41701 ctttataaag aaaagaatca tcctgtggat tgatacctag ttcttcatta tagggtaaaa
    41761 acatcttttc agctgactct tgccattctc taatttcatc gggcaacagg ttaatcgcct
    41821 ttttaatctt gtttaattta tctcggtttt cttcttttaa ttcttgatag acttcaacag
    41881 catactctaa attaaatttg gccatatagt ttgtataaca gttattgttc acggcaggct
    41941 tgtattcatc aggaccacaa gcctcgttga tacagaactg accttctttt agttcttcat
    42001 agtggcctaa actcaaccac attcgggctg tttcaaataa tatctctgct cctttattgt
    42061 acaagaaatc tttatctcct gttgcctgga catagttttt gatagcaaat gcaatatcag
    42121 cattgatatg atactggaca gtagagccca aaaagttgcc ggaggcttct tccccattga
    42181 tcgttctcca aggatagaga gttcctttta acttcattct ttcagcatta tcacgggctg
    42241 aatccaatat ttcatagcga taatttaata actgcttggc tatttcggga tgactgtata
    42301 agaaaaacgg tattatataa gtttcggtat cccaaaaata atgtccttca tagtattcac
    42361 cagttaaacc tttggccgca atatttgtct taccatcttt accggtagac tgcaagatct
    42421 gtaaagcatt aaatctaaaa gcctgctgta gggcttcatc tccttcaatt tttacgtctg
    42481 aatactgcca gtaatcttta agatattcta tctgactggc ttttatctca tcaaagccca
    42541 tagctttggc tctttttgct gactgataac attgagccaa tatctcttct ttttcgatct
    42601 catctcttga ggtataaaag gctgcatact tttctaatct atatgtttta tcctgcttag
    42661 catcgatctc atacacttct ttaatatttt tcccttcaat attttcctcg cgtaacaaga
    42721 atttttcttc gattcccatc agtttattat agatagtact ccctattgaa atatcagaat
    42781 ttttagtagt taccatcaga taggaaaact gactatcaat tcccttttgc tcaattaaaa
    42841 gagggcttct atctctcaaa tgatgagaat tttgcacatc acctattaaa gaactctcga
    42901 tttttatttt accgttaaaa tttaaaggag taaccgcgta cttaatagtt cctatatgtt
    42961 tatgggcaag agaaataatt ctttctattt ctatattaat ctttttacca ttgggtgaag
    43021 tccacactaa agatcttttt aataacccct tttttaagtc aagatatctt tcataaccct
    43081 caactttacc ctctaacata ttaaacttct catcttcaat atataaattt attgttttcc
    43141 agtccatgag attgatcatc gtctgataat tttgaggttg tttgggtgca aactccccat
    43201 aaattatctc ttcttcttca tatatcccgt taatataaaa acccggtgtt gtagaatcgg
    43261 aggttcctgt ataatcttct tctaaagttc ctcgtaaacc catataacca tttcccaaag
    43321 aaaagatagt ctcattatga tggttgtttt caacttcaaa ttcttgttca gtaattctcc
    43381 agggttcata gtcatataaa ggtggttttt cgctttttaa tctatggtaa gttaacatat
    43441 aatagacctc cttatctact tattttttaa catattaatt cctcgcggaa tcacatcaat
    43501 aatagaatct aagcagtctt taaccaactt ggggtcaccc ggcagattaa taatcaagct
    43561 ttctttatta attccggcag tacctctaaa tatacaggca ctgggcctgt attcagagac
    43621 aatccgtctc atcttttccg gaatacccgg cacctctttt tcaattacat ctttagtagc
    43681 ttctggtact aaatctttag gagatagtcc ggtacctcca catgttaaaa ttaattctgc
    43741 ttctctattt acacttaaat cgaataattc ttctttcgtt ttttcatatc tattatcaat
    43801 agatttaaaa tatacttttt ctccttcgat ttgagatgct aaatttctga cgatttttct
    43861 cgtctcctct tcttttttct cattatgttt actatctcta atagttaata gtgcaacttt
    43921 taccaattat attcaccctt tcaaaagtat tatctattcc atccactatt tttctgtgaa
    43981 attaagagat aagcaaaaaa tggtgcacct aataaggcgg taatgatact aattggtatt
    44041 tctgctcctg ttaaagacct cgctatatca tccattaaaa gtaaaaaact ggctcctaat
    44101 aggccagaag ctggtaccat atagcggtga tcggccccca caataaatct ggcaatatga
    44161 ggtactacta atcccaccca gcttacttga ccaacagtag ccacagaagc agctaccatt
    44221 agggtactaa ataaaaccat aacatttcta acaagtttca cattaatacc catcgatatg
    44281 gcttcttctt cccccattga catcacattt aaataccagc ggatcaatac taaaattatt
    44341 atacctataa taataaaagg ggcggcaaac ttgacttctt cccaaccggt tcggctgaat
    44401 ccgcccatca gccaaaagac tatccccggc agtttttcat atgggtcagc catgtatttt
    44461 aaaattgaaa taaaggcatt aaataatgaa cccaccacaa tcccggataa gaccatcatc
    44521 attatcatat tagttttacc taaatttgaa ataaaatatg ttaaaaatac cgctatcaca
    44581 ccaaacacaa atgctaataa ataaattgaa ctaaaagatt ggaaggataa aatcataccg
    44641 agggcggccc caaaacttgc tccggccgaa acacccaaaa tatcaggtga aaccagtgga
    44701 tttctaaaaa tactttggta actggcccct gctactgaca gggcagcccc tacacccatt
    44761 accaataata tacggggtaa tcttatgtaa tagaaaacgg actgctccat ttcatttaaa
    44821 ttaaacccat tgataaattt catgaagggt atattaaatc tccctataaa taaagaaacg
    44881 aacattatca aaaatgtcag aacggtgatt attattaatt tatttctatt gttttttctc
    44941 tcgtctaatt gccgcatacc ttaccccacc taaatgtaca atttcttttt tatagtttat
    45001 attatatctt ttctatttac aaattttttc gcagtgtata atgtactatt tcacggacat
    45061 gcagctcaag gctatctata attctaacct cacgcgcagc tataatatca attagggaag
    45121 agaattcagt acaacctaaa attattgcat ctacatcctg gaacattaaa ttatcgatta
    45181 tgctattgat atagtatctc gaactaatat ttatattatt acagaccagt tcatttatta
    45241 tgatttcatt tagttcatca atctcccggg gatcaggggt tactactttt tgggcaaact
    45301 ctgtttctaa ctcatcctgg taaaaaccat tattaattaa atatttagtg gccagtaaac
    45361 caggcctttt acaccaggtc tttttgaggt ttttacccac tacgtcaatt atacttaaaa
    45421 tagggatgtt tatattattt tgtatctcat cttcaatttt gtgaattgta ttggaagtta
    45481 tcactaaaaa atcagcgccg ccttcttcaa ttttatttgc tttattaatc atgatctcag
    45541 cgatagaatc ccaatcatct ttttcaagta gtttaactat ttgatcaaaa ttaattgaat
    45601 aaattattat ttcagggtta atattattct ttctgatccg gtttatatat ttatttatcc
    45661 tattataata aagggaagtt gaataccaac ccatcccacc caaaagacct atcttcttca
    45721 cttcttgtac cccctaaatt catctaattt aactgcactt ttaatttttc ttgacttttc
    45781 tgctgctaaa gcggcaaagt gactttctaa ggctgacatc aaattactat catttttatc
    45841 taatttattt gataaaaact ttgcaaaaga ctcgattagc tttttatctc ccccagcatg
    45901 tccaccttta tactcaatat ttattttata atgcttatct aaaaaaggct ttacttcaat
    45961 aatccccttt tctaaatcac caaatatttc accatgagtt ccaaaaatat ttatctttct
    46021 agtcatatca tttgaaaaag cagtcaaagt aaaatgggca gtgatttcat ttttaaattt
    46081 taataatagg gtttgtacat ccagctggtc attatcactt ttatagacac atttaccata
    46141 attcgtctcc tttagagcct ttgtaatacc ctcttcacta aaatcatctg taattacatt
    46201 taccggccaa ccgaagggaa cagcaaagta taacttctta gctgaatagg ggcagttatc
    46261 ttcaaccgga catgttaaac aacgctcccc tgcctgaggt agatcatatt ttttatcaaa
    46321 atatctttct gatgaatccg aataaatatt tttatatccc acttccatca gccaatacaa
    46381 aatatctaaa tcatgacagc tttttgctaa aataataggg gcgctcacct cgtggttgcg
    46441 ccagttaccc ctgacataac tatgaccata gtgaaaaaat ccaatatttt caattagatt
    46501 aataaatctt attctcccga ttatattttt atttaatagc tcttttatct ttttaaaaaa
    46561 aggtgtatat cttaatacat gggagaccat aacctgttca ctatcttcac tgtaatcttc
    46621 ttgtaatgtt tttagttcat taaagttatc agtaattggc ttttcacata gtactttata
    46681 gcctttcgac agagcttttt tcaagggttc aaaatgtatt ttatccccgg ttgaaattat
    46741 tagaccgtcc gctaatctct cttcaaataa taaatcctcc cagctttcaa attgataatt
    46801 gggaggaatt ttatgttctt ttgcaaattt ttttctgcgg tcttctaggg gctctgcaac
    46861 tgctgatatc tgtaaatttt tattttgttt aattaatttc ccataaacat ctttacctct
    46921 gttcccggct ccgagaacag ctacactctt ttttgaaatt gtaatcaccc ctcagcagaa
    46981 atactattat taatattctt tagattttaa atatttcctg cctctctagt gacgaaagtg
    47041 gcgtatattt gtaaagtaca tcgctattcc gagttcatca gctttttgaa taacctggtc
    47101 atctctgata gaaccaccgg gctgtatgat ggcctttata ccatattcag ccgccttttc
    47161 aaccgcatct gggaacggaa agaaagcatc ggaagctact actgccccct gctgtctacc
    47221 ctgggctttc cgcccggcga ttatcatggc atctacacgg ctcatctgac cggctccgac
    47281 tcccaccagc atttcttctt tagccatcac aattgcattt gatttaacat gttttactgc
    47341 tttccacgcg aataataaat ccttaattaa attactttct acaccttttt ctgttactag
    47401 ctgcaggtca tcagaactaa ccatctctaa atctctatcc tgcattagta agccgccggt
    47461 tactttcttt agctcataac ctggttcttt atggtttata tacaaatcat cagtttttaa
    47521 aattctaata ctttcccatc tatttgttaa aatatcaacc gcttccttac tataatcggg
    47581 agctgtaata acttcgatat acttttctcc actaactatt tcttctgcta attctttgtt
    47641 aactgaccga tttaaggaaa ctatggaacc gaaagctgac aggggatctc ccgcatgtgc
    47701 tcttttaaaa gctgttatta tatcatcggc tgaagccaga ccacaggggt tagtgtgttt
    47761 tattaccgct gccgtcggtt tatcctgaaa ttctttgact aattctatag ccccattggt
    47821 atcatttata ttgttaaaag ataaggcctt tccacttaat tttttagcat tagttataga
    47881 cggttcattt acctttctgg cttgataaaa tacagccgac tggtgggggt tttcaccata
    47941 acggagtttc tgctttttat aatatggttc aagtaatata tcaggccatt cctctttttc
    48001 ttctagattg tcaaagaaat atttttgaat gatagaatca tattctgcag tatggttaaa
    48061 agctttataa gccatcttca ttctaaaaga cttatctatt ttttgattgt cttttaactt
    48121 ttttgcaact aaactatagt cttttggatc tactattact aaaacatctg gaaagttttt
    48181 agctgcggcc ctaatcatag tcggaccacc aatatctata ttctctaata cttccttaag
    48241 ttcaacctct tttttatcga tcgtttgggc aaaaggatat agattacaaa caatcagatc
    48301 aatagttttt atatctagac ttttcaattc tgttaaatct gcttcgttat ctcttcttgc
    48361 caggatacct gcgtggatat tagggtggag ggttttaacc cggccgtcca tcatctccgg
    48421 aaaatcagtg acatctgaga tgttaataac atctatgccc gcttctgcaa tcctgttggc
    48481 agttccccct gttgagataa tttcaatatc ctgggctgct aaactttcag caaaatcttt
    48541 aatacccgtt ttatttgaaa cacttagcaa ggctctttta atttccgtca attaaatcac
    48601 tcctttacca aaactgtatt atcatctttt aatttaacct tatcttcagc tattaattca
    48661 ataacagccg gtaatatttc atgttcatgt tttaagatcc ggtctgctag actttcttca
    48721 tcatcgctgt ctttgactgg tactgccttt tgtttaatga tcgggccgga atctacacct
    48781 tcatcaacaa aatgaacagt acacccgctt accttaactc cataatctac agcctgtttt
    48841 tgggcattta gacccgggaa agcaggtagt aaagagggat gaatattaat tattttattt
    48901 ttgaaagtat ttacaaatct tgccgacaat actttcatat aaccggctaa tactattaaa
    48961 tcaatattgc ttttttgaat tgcctcaata accttgtctt catagtcctt tttattatta
    49021 tacctatttt catcaattaa ataaacttta attccacaat ttttcgctct ttttagggca
    49081 taagcctctt ttttattact gataacgctt tttatctcaa ttggtttttc gcttaaatgt
    49141 aatttatcta ttatggactg caggttggtg ccgcggccgg aagccaaaac cgctaagtta
    49201 aatattttat cattcattta aaatcaatcc tttgtcacct ttatctattt cacctatttc
    49261 ataggctgtt tctcctaatt ctttcaatgt tttaagaact ttatctatat cttttttatt
    49321 aactacaaga accataccca gacccatatt aaaggtccta tacatctcat ctgactctat
    49381 attacctttc ttttgtattt tcttaaaaat ttcaggggtg ggaaaagtac tagtagcgat
    49441 ctcggctgta tatcctgttg gtaaaatacg gggcacattt tcttttaagc ctccccccgt
    49501 aatatgggct atccctttta tattatactt atctattagt tctaatattg gttttacata
    49561 tattttagtc ggttttaaaa ggttttctaa taaactctgt tcattttcct cattgttttt
    49621 aataaagagc ttcctaacca gggaatatcc attactatgg atcccattgg aagcaattcc
    49681 aatcaaagta tctccttttt taatctcaga cccatcaatt acttcttttt tatctactat
    49741 gcccacacaa aaaccggccg tatcatattc accatcggga tagaaatcgg gcatctcggc
    49801 cgtctctccc cctaataaag cacagccagc ttctttacaa ccctgcacaa taccggctac
    49861 tacttcatta aactgtttca catccaaatt accggttgcc aaataatcta aaaaaaacag
    49921 gggctttgca cctaaggtaa ctaggtcatt tacactcata gctaccaggt caataccaat
    49981 cgtgtcatgt ttatctgcca aaaatgcaag cttcagtttc gtacctaccc catcggtagc
    50041 tgccgctaat atgggttcat tatattcctt taaatcgaga ttaaataaac caccaaatcc
    50101 acctaactct gttactacat tctcattata ggtggatttg accatttcat taatttgttt
    50161 aactgcttct tccccttttt taatatcgac cccggcatct ttataggata aacccatttt
    50221 tatctacctc tctcttttct ttttctgcag tcggatactt accattaaag caggcactgc
    50281 agtatccata atcatctgct tcaagaactg aaagtatacc ttctaaactt aagtaatgta
    50341 aactgtcagc ccctatatat tccgcaattt cttcggtttc tttttttctt gcaatcaact
    50401 ctttacgacg ggaagtgtcc agaccgtaaa aacaggggtg tttaacgggc ggggaggcaa
    50461 tcgccatatg tattttatta acatctgttt cccttaatct actaattatc tgcctacttg
    50521 tagtaccccg gacaatagaa tcatcaatta aaataatatc ttttccttct aatattttat
    50581 taattggatt tagtttcatt cttaccttca aatctctcat ctcctgggtg ggctggataa
    50641 aggtccttcc cacataacgg tttcttaaaa taccgtctgc ataagaaata cccgattcta
    50701 aagaatatcc ctgggcagct gccactcccg aactaggtac gggaaccacc aggtctgcct
    50761 ccatgttcat ttcccgggcc agtcttctcc ccattctttt ccgggcaaga tagacatttt
    50821 ccccaccaat tatactgtca ggccgggcaa aatatataaa ttcaaataca cagaaggctt
    50881 ttggtttatc ttcactgtat ctttggcttt taatcccatc ttcatctatt atgaggactt
    50941 ccccgggttt aacttctcta ataaattcag cgcctataat gtcaagggca caggtctcag
    51001 aggataatat aatgctgtca ccctttttcc ctaaaatcag gggcctgaaa ccatgggggt
    51061 cccgggcagc aattaagcta tcattggtca tcgcaatcaa actaaatgcg ccctttatct
    51121 gctggagact ctgtattaag gcctcaataa tatcatcctt aaatgatctc gctaccagat
    51181 gggcgattac ttctgtatca agagttgaat ggaagatgga accgtgtgat tctaaattat
    51241 cttttagttc ccgaccatta attagattac cattatgagc tagggctaag tcccctttaa
    51301 tactatttgt taataggggt tgggcattta ctaaaagtga ggagcccgtg gtagaatatc
    51361 taacatgacc tatactaata ttgccggtta aatctcctaa tatatcgcta tcaaatacat
    51421 tactgaccag ccccatgcct ttatgaaccc ttaatttgtt ctcatcattt acacagatac
    51481 cggcactttc ctgcccccgg tgctgtaagg catggagtcc gagataattt aaacgggaaa
    51541 tatcatctct tttttcaggt gaaaaaatac cgaagatacc gcactcttct tccatcttat
    51601 cacttttctt taaagctttt tctcgattgc agtttcccaa ttttttttca tctccttaac
    51661 attcaactga attaaatcat tgaacttgaa tatacttctc ttagtttggc caatttgttt
    51721 taaaggaata ctattgttat cagctatttc attaagaagt tcactatcag cggggtcaat
    51781 acttactact attcgactct ggctctcacc aaataatagt gctttatcac tgatattatc
    51841 ctttaggtca acatctatac ccagttcgtt ttcaaaacag gattcagcca aagcaaccgc
    51901 caaaccacct tctgaacaat catgggctga ttttatgata ccttccttaa cagcctctct
    51961 tactaatctt tgcactcttt tttctaactt taaatctatt tccggtacat atccatctat
    52021 cttatcttct tttaaggcaa g
//
LOCUS       DATGCJ010000127        28519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_417792, whole genome shotgun sequence.
ACCESSION   DATGCJ010000127 DATGCJ010000000
VERSION     DATGCJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 28519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28519
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417792"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..130)
                     /locus_tag="VKN64_06265"
     tRNA            complement(41..130)
                     /locus_tag="VKN64_06265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Leu,seq:taa)
     gene            complement(180..1505)
                     /locus_tag="VKN64_06270"
     CDS             complement(180..1505)
                     /locus_tag="VKN64_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMA59881.1"
                     /translation="MDYTHDLLEIQHWMMVHHKSYKVIFIIGLLLLFTFLIHTFTEKY
                     RLPSVAGYVIIGAIFSASVVEHIPFFSTEFTEWYTYLINSFNFVTILAVSFISFTIGT
                     SLSLKVLKKLESEFTLIILLESIGAFVLVTMSMLYIGKPLYLAILLGAFATATAPAAT
                     VLILREYGVQGEFSATLMIVLALDEALALVIFSFVEPISLISIDPDQTLNVLNGFIIP
                     TVEILSATGLGLLFGYLTQRYICFDHSKKQKVFLILLTIVGGSALSIALHLSPLIYNL
                     FVGFAFCNFAEKHIGVADLIDTLTMPLYAIFFILAGTKIQITNIFTSSFMIIAIIYTV
                     ARLTGKIGGATLGARLANAPQSVQKYIGFGLMPQIGVALDLAYVVQRDFAHIQNTEVD
                     VAVIIFNIILFTSLFTEISGSAATEYALFRSGEIKEPEEEEPKFSNPLT"
     gene            complement(1520..3118)
                     /locus_tag="VKN64_06275"
     CDS             complement(1520..3118)
                     /locus_tag="VKN64_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMA59882.1"
                     /translation="MLTKIEKLWQQLYGEEKEYLNKLKELLDKRKDEVDYQPNNENWH
                     QEGIVYSLYVDLFADDFQGLIDKLDYLDELGVNTLWLLPILESPMVDQGFDISDYYQI
                     REELGTNQQFFDFLEKAHAKNIKIVFDIAVNHSSDQHEWFKDARQSKEARYRNFYIWN
                     EDKQKYTETRLLLKGISNSNWTYNEATDDYYFHRFYDIQPDLNYKNPAVLYEMLKAFT
                     YWRAKGVDGFRMDAAPFLWKKEGTDCENLEETHKILKIFRAAFDYIAKGTVMIAEANQ
                     MPSDVIEYLGEGDECHVVYNFPIMPRIFLSIAEQESDYLEKQLEIIGKLEEPPGTAWF
                     TFLRCHDELTLEFVTAKERELMNEHYLKDERWTFREGEGISGRLYDLLDKDVSKVLLA
                     YSILFSIKGTPIIYFGDEIGMENDLDFYNKMNEKYGYKDSRYLNRGPFNQEKKTTALK
                     QPESDPGLIYRGLKEMIRLKNENLKLFKSEVEYETKGNLLISKRQAEDKKLTIVNNLT
                     EEEKSFKDYQLKPYDYLWILEENT"
     gene            complement(3133..4038)
                     /locus_tag="VKN64_06280"
     CDS             complement(3133..4038)
                     /locus_tag="VKN64_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HMA59883.1"
                     /translation="MSKAVIEMGTNTTRLLIAEQQEQKIKELKKDLITTRLGEGVDSN
                     KTLSENAIKRGLKALKSLQREIDQYEVSSVKLVGTSALRDVNNADKFQKLIKEEIGYD
                     LEIISGHKEAELILKGVTYGYNYSDYLIIDIGGGSTEFIWKSEEDKKTKMDSLNIGAV
                     RLTERFIDNPHQEWSRSDIKNIAEYVEQELEKELDILPGVQNLIGVGGTITTLAAILL
                     KLEEYDQQAIHDYILNYPDINKVLDRLRKLDLEKRKKVKGLNPDRADIIIAGIVILNE
                     IMKTLRTLKLRVSDYDILHGLIIES"
     gene            complement(4031..4573)
                     /locus_tag="VKN64_06285"
     CDS             complement(4031..4573)
                     /locus_tag="VKN64_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HMA59884.1"
                     /translation="MALKKSETYHIIKKQIEREPAGLVEVSINCPFGYPAVVKVYPFF
                     NKRVFPTTYWLTCPYLNKEISKLEDQSWIEKIQDKVNNDNALIEKLDKAHANYAQIRM
                     AMLSQEEIDQIKEISEDIIYTLKHSGVGGIKEKKGIKCLHTHTADYLVNKENPVGKIV
                     FDKTGWPDKCKICEELKKDE"
     gene            complement(4758..5126)
                     /locus_tag="VKN64_06290"
     CDS             complement(4758..5126)
                     /locus_tag="VKN64_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HMA59885.1"
                     /translation="MSVEVGSTVKGEVTGITNFGAFIELPNGETGLVHISEIANTYVK
                     DIGNFLEEGEEVKVKVINIDDDGKIGLSIKQLKDPSDRIDHAPNKSFEEKMDEFLQQS
                     SERQSDLKKRVEKRSDSGRN"
     gene            complement(5211..7364)
                     /locus_tag="VKN64_06295"
     CDS             complement(5211..7364)
                     /locus_tag="VKN64_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HMA59886.1"
                     /translation="MGKIAKKISDKLEIKQKSVKSVIDLIEDGNTIPFIARYRKEQTG
                     GLDETQIRDVNEEYEYLTHLFERKEEIKNTLSKKEKLTEEIEEKLEQAETLQEVEDIY
                     RPFKEKRETRATKATDRGLKPLAEYILAGYEKNKLNQKAEEFIDEEKEVENVEDAIQG
                     ASDIIADMVSNNNVVRKNLREEMKERSLITSQKDEDIEDEKNKYELYYDYKEKIAKIP
                     DHRVLAIDRGESDEVLKVSIEHDEEKMLEIIKDTILSEEMAVESEILDDTIKDSFKRL
                     IEPSLNRELRNLLTERAQAGAIEIFKDNLRSLLLTPPYKGHKILGIDPGFRTGCKAAA
                     IDEMGNFLEYKTIFPHPPQNQKEKAAQKVKNLLNEYNIDTIAIGNGTASRETEMFVTE
                     MGGDIDFKYIIVNEAGASVYSASEEAIKEFPDLDVSVRGAISIARRLQDPLSELVKID
                     PRSIGVGQYQHDVNQTELKESLDQVVESVVNQVGVNLNTASASLLQYVSGINSSVADK
                     IVNYRQENGKFKKRKEIKDVYGVGAKTFEQASGFLQFFSKKDPFAYTFIHPESYEEAE
                     LILEKLGYQPADILAEDKLKQLREDINDLNKKEVISQSEIGEFTLDDIIKNLKKPGLD
                     PRDELPDPIFKNEVLKFEDLEVGMVLKGTVRNVVDFGAFVDIGVKQDGLIHISELSYK
                     YVDHPLNVISVGDTVRVKIIDIDEKRKRISLSRKL"
     gene            complement(7364..7708)
                     /locus_tag="VKN64_06300"
     CDS             complement(7364..7708)
                     /locus_tag="VKN64_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMA59887.1"
                     /translation="MSFETFFKKAMVYLLIIVIVVFAFKLMMNIRKVNQMEDRLNSLQ
                     QQVQNQIEENKELKEEIERVRSPEYVEKVAREELGLVKPGEILLIPVEEEQDNNQEEN
                     TQQQNSEEQDDN"
     gene            complement(7726..8238)
                     /locus_tag="VKN64_06305"
     CDS             complement(7726..8238)
                     /locus_tag="VKN64_06305"
                     /inference="COORDINATES: protein motif:HMM:NF021439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HMA59888.1"
                     /translation="MDIMETAAELMAVSARTAPKTAGQDYVEIKVIGGDKIEKLADEM
                     INYGKESGKGNFDRDGENVRNSEQVLLLSLNEAAVIGLDCGACGFDKCEELKLKSGPE
                     FDGPICAWRLMDLGIALGSAVKTASLLNVDNRIMYRIGVAAKKMGLIEGEIVVGVPIS
                     ASGKNIYFDR"
     gene            complement(8314..9423)
                     /gene="ugpC"
                     /locus_tag="VKN64_06310"
     CDS             complement(8314..9423)
                     /gene="ugpC"
                     /locus_tag="VKN64_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HMA59889.1"
                     /translation="MASVTFENVTKRFGDVVAAKDQDLEVKDKEFLVLVGPSGCGKST
                     DLRMIAGLEEITEGKIKIGDTVVNDIPPKDRDIAMVFQNYALYPHMDVYDNMAFGLKL
                     RKFPKDEIERRVQEAAEILSIEPLLDRKPKQLSGGQRQRVALGRAIVREPKVFLMDEP
                     LSNLDAKLRVQMRTELSKLHDRLKTTVIYVTHDQTEAMTMGDRIVVLKDGYIQQVDSP
                     LELYNHPNNMFVAGFIGSPAMNFMDAKIEKDGDEYFADGFGAFRIKVPEQFTDRIKDY
                     AGKRVVFGIRPEDLTDARITHDLDITPENSFEAEVEVVEPMGSEIYLYLAVDEHEMIA
                     RVDAESDAEVGDKIKLGVYTKKMHIFDNETEEAIF"
     gene            complement(9427..10443)
                     /locus_tag="VKN64_06315"
     CDS             complement(9427..10443)
                     /locus_tag="VKN64_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA59890.1"
                     /translation="MPLTLKDIAEMVGVAESTVSRAINGKPGVGEKTRKEILKIAREY
                     NFKPNKLAQGLAKQETHILALLLPGLSQGNYSQIIENIESVAEAAGYQIILCNTDNDL
                     EKEKSYLDLLEEKRVDGAIILGGEIADQHIVNTKLRGHDNIVLVNRLCEEILIPTILI
                     DSSQGAYLATNHLLDLGYENIALITGNSNDYIESEKIKGYKNALVDHGLNFNSNLVIE
                     TDGTREAGYQAFLNLVDLQTPPDSFFVTDELLSVGLVEAIKMGGYFIPQDYPVVSYGD
                     TIINSIVEPELTRVKEPLSQAGRMAAEVLINLINNEEAEEEIKVLKPELKKCESTKIN
                     KGRN"
     gene            complement(10535..11110)
                     /locus_tag="VKN64_06320"
     CDS             complement(10535..11110)
                     /locus_tag="VKN64_06320"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA59891.1"
                     /translation="MDEHGRLFNNIAPYYQLFFKYQVWSYGNIVDENISLIKDEKIDT
                     ILDIGCGTGAFGYVFQEKGYSVTGIDVAPEMVKRGINNDLYCEKGDVNEGLSYSDNSF
                     DMVISAMVAHGLDEKTRQKFFNETKRIAKNVVLIQDYKFQRKFYVDIIEKLEGGGYFN
                     YIENGLKQFNSIFDNLSIKEVSRNLNWYISK"
     gene            complement(11131..11781)
                     /gene="pgmB"
                     /locus_tag="VKN64_06325"
     CDS             complement(11131..11781)
                     /gene="pgmB"
                     /locus_tag="VKN64_06325"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HMA59892.1"
                     /translation="MAEIKGFIFDLDGVITDTAEYHYLGWKKLAEEEGLAFDREFNEK
                     FRGVSRMECLDLLLEANDRKVTPEERKELAERKNNYYREYLKSISQDNLLEGIEEILK
                     RVKKDGYKMAIASASKNTKIVVGKLDIKDMFDTISDGYSVKNSKPAPDLFLHTAEKMD
                     LKPAECVVFEDAEAGIDAALAANMTAVGIGPDSRVGHAHYRFDSVKDVDLDEIIGS"
     gene            complement(11796..12371)
                     /locus_tag="VKN64_06330"
     CDS             complement(11796..12371)
                     /locus_tag="VKN64_06330"
                     /inference="COORDINATES: protein motif:HMM:NF021952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase"
                     /protein_id="HMA59893.1"
                     /translation="MIKNSSFKDEYFIILKPEGDVLDLAQYLQDKLHSQFDLYPDDMY
                     PEIHITLDRIYKDRVEEAIEIINSLTLKYAKIDILVKNFDCYSFKENNFLVLNVEENK
                     QLVQFSNELHDKLAEKNISTIANYEDWKFHISLASTVFTEERQNFDQAFQNLCYRFEG
                     IHTPRECKAEVIEIWRPTLDEDIKCLKQFQL"
     gene            complement(12364..13635)
                     /locus_tag="VKN64_06335"
     CDS             complement(12364..13635)
                     /locus_tag="VKN64_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59894.1"
                     /translation="MLKLENINFSYAKQKILHEINFHLKEHEFIGIIGPNGSGKSTLL
                     KNISKLLDPDEGYIYLDKNLLNDYSYQELAKKMAVVPQDTNVSFNFTVYDLVMMGRNP
                     YQDRWGRVSKEDKKKVEEALQLTDTAKFKDKSIKNLSGGERQRVIIARAIVQEPELLL
                     LDEPTASLDINYQRNIFDLISDLNEELEMAVLAVSHDLNLISQYCERLILLHKGKIYS
                     MGEVEEVITKANISEVYNTDVDIKYNPITERPYVIIIPRQKGSQQMRAHDFKVHLICG
                     GGTGNDIMNILNDLNIGVSTGVLNRGDSDWESAKRFNFEVVEIPPFAPIDERAVQENK
                     EKMQKADLIIISDTPFGWGNLDNIKVLADFNDKDILFMMNNDLQKRDFTEGKFTELYK
                     DIKENNNVNEIDSNKKLCNFINSKYEEILYD"
     gene            complement(13642..14655)
                     /locus_tag="VKN64_06340"
     CDS             complement(13642..14655)
                     /locus_tag="VKN64_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HMA59895.1"
                     /translation="MEKKTNYLYIILAIIALLFVVGIISLSFGSINLSPLKVLKIIFF
                     GEDVKQSLNVIIKQIRLPRIILSFLVGAGLSIAGVVFQGVIRNPMVDPYIVGISAGAG
                     TGVTLAIVLNLQISFLFFNTIPLMAFFGSLITVYIVYNLAKTKDKVPVVTFLLAGVAV
                     GFVLNAVMSFFMVIGTRDLSKIVYWLLGSLSTASWQDIRLMLPYFVVGNFIIIFFLKD
                     LNLILLGERDAQHLGVDVEKSKRYLIIGASLVTASVVSVSGSIGFIGLIVPHIARMLV
                     GPDHKKLFPTAAILGGIFLIVSDDLARILLAPMEIPVGIITALTGGPYFIYLLRKQKK
                     EIW"
     gene            complement(14769..17084)
                     /locus_tag="VKN64_06345"
     CDS             complement(14769..17084)
                     /locus_tag="VKN64_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HMA59896.1"
                     /translation="MSISYEAGEDFYKNWIIEETAFKKENQAKFETIFSLGNGYMGLR
                     ASAEETYPKERRGFYIAGIFDEFSGEVTEMPNLPDWIRTEIYINGELFKLDKGQIFEY
                     SRKLNLKDGLLIRDFHWESPNGEMVEFSFERFISLNNLHTGVSKIEIKPLNFSGEIKL
                     ISGFDGRVSNSGTQHLKEGEKRLIEDYIVYQPETQQSEIKMSFGLHHDFYKNKEKFEP
                     QKDFKAERRRIKRVYEFNLKQKEKATIIKRLSAFTNKDKDFREDEDLEDKMIDNLINI
                     RDTAYKELKENHLQKWRSVWEESDFKISGSDFDQLALRFAIFHLIQMIPVHDDRISIA
                     AKGLSGEGYKGHVFWDTEVFILPFFIYTHPEVARNLLKYRYHTLAGARQKAQNNGYQG
                     AMFAWESAETGQETTPKYGAVDILTGEEIRIWCGEIEQHITVDIQYAFDQYFQATGDI
                     EFMKEYGFEVFLASTRFWASRLEYNQGQDRYEINNVMGPDEYKEHIDNNAFINYMVHW
                     QFEKALAYIKYLKKEEEAYLNEIKDELNLKEEEIKDWANKRDNIFIPKSDDNNIIPQF
                     EGFQDLKEIDLTKYKQGDVGDIFDDLGWEEITESKVSKQADVVMLTHLLEEVFDEEII
                     KANFDYYEPLTLHDSSLSPGIHAAVAAKLGEIDKAYNLFQRAARIDLGRNMASSDAGL
                     HAASLGALWQSVVFGFAGLRLKGDVLQIDPHLPNRWNMVSFNINVKQNKYKVSITQEN
                     IKIEAINIKEIIPIQVNNQVYEIEDSQKIKY"
     gene            complement(17150..17225)
                     /locus_tag="VKN64_06350"
     tRNA            complement(17150..17225)
                     /locus_tag="VKN64_06350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17190..17192),aa:Gly,seq:gcc)
     gene            complement(17248..17332)
                     /locus_tag="VKN64_06355"
     tRNA            complement(17248..17332)
                     /locus_tag="VKN64_06355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17296..17298),aa:Tyr,seq:gta)
     gene            complement(17512..17706)
                     /locus_tag="VKN64_06360"
     CDS             complement(17512..17706)
                     /locus_tag="VKN64_06360"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA59897.1"
                     /translation="MSDKPEIKGRPTDFTVSVSQVTLSAGAGFLVPSTGPVLKMPGLP
                     KRPSAEDIDIDENGKITGLF"
     gene            complement(17643..17891)
                     /locus_tag="VKN64_06365"
     CDS             complement(17643..17891)
                     /locus_tag="VKN64_06365"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA59898.1"
                     /translation="MSIYTVKNSEEFSIKEKIDKIAKVIYGADGVDYSSAAEKQISTF
                     EKQVMVIYRSVWPRPKTPYLISQRLREDRLTLRSQSPR"
     gene            complement(18928..19209)
                     /locus_tag="VKN64_06370"
     CDS             complement(18928..19209)
                     /locus_tag="VKN64_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59899.1"
                     /translation="MDKANPLDLINSEKTAIDASKLKSYERAIPKSHFDENDCGNINW
                     GTKFDSLKKQDILVQLKVYLTVDTKSELTIAVNFPRIMKLNVIRHKKGK"
     gene            complement(19971..21164)
                     /locus_tag="VKN64_06375"
     CDS             complement(19971..21164)
                     /locus_tag="VKN64_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMA59900.1"
                     /translation="MDQLERKLFATSEEISQELIELRREIHKNPELELDCYETSKLLL
                     EKLKGLNLDIKHGIANTGITALLKGKKKKPVLAIRADIDALPIEEETGLPFASQNKGK
                     MHACGHDGHATLVYGAAKILSEHRDLLEGSIKFIFQPGEEYPGGAKIMIEEGVLENPK
                     VDALISGHIFPGIKNGKIGIRYGALTARNDEFTIDIMGEGGHGAHPDSTQDTIVTSSL
                     FIVELQTIVSRITDPVKPLVINVGEISGGGGHNVVPKKITLKGTIRSIDKKTRDIAIE
                     KIEKLLEGLKMSYNIKYNFELVPGEPPLYCDENLTKNTENILMKILQDKDLVKIDEPS
                     LGSDDCAFFSERIPVVYIRYGSFDEKKGYTNDLHTPSFDFDENLLLKTSKILSYLSIN
                     LLKKI"
     gene            complement(21223..22569)
                     /locus_tag="VKN64_06380"
     CDS             complement(21223..22569)
                     /locus_tag="VKN64_06380"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HMA59901.1"
                     /translation="MEKEDILKIVEEEDIQITRLLYIDNDGIIRGYSSKKESLESAVN
                     QGFCIASGMPYFTAFDELVDGSNLGAVGEFRIVPDLDTFKVLPYARKNAALICDIKEQ
                     TLEDSGVCARTILKNIIEDSEFEPYAAFENEFYFLKHDEQGELQPADNSICYTTKGMN
                     EMNEITLDVLEALDKQNIEIEKYHPEYGPGQQEYCLKYDKILNMADKQIFFRDTIRGI
                     AEKHDLISTFMPKPFNGLAGSGAHINLSLWKNGENIFYDENDAHALSETAYYFIGGVL
                     KHIKAVCAFTASTITSYKRLQPHNWASAYVCYGHNNREAAVRIPSTDFNRQMQSKDST
                     RIEFKPNDPSSNPYLALAAVLTAGLDGIKNKIDPGESVEIDPSNLSEEEKETKNVSSL
                     PRDLSRALDALEEDKLFREVFGETMISEYLKMKRQDWSDFMNHVTSWEKDKYVEIF"
     gene            complement(22591..23706)
                     /gene="alr"
                     /locus_tag="VKN64_06385"
     CDS             complement(22591..23706)
                     /gene="alr"
                     /locus_tag="VKN64_06385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HMA59902.1"
                     /translation="MEGYRTWLEINKEAIYENVDKIKGDFLKDQYLMGVVKGNANGYG
                     TVLYSQTLQKAGVDWLGLTNIKEALTILKEGIKLPILILCDIPYSYINIALKKQIRLT
                     VYDKRTVKRIKNKAKNLNLSAKVHIKINTGLNRIGVSPDNFTKLAETIKNSPHMEIEG
                     AFTQFSTTHHFKKEKTRKQLNLFLGLIEEAKNIGIDIPILHAASTPSTLKMPETHLDM
                     VRVGIGLAGLYPSKEYRKIIDLKFPLAWKSKVCQVKLVKQGEYVGYDDGYRCQKKTLI
                     ATIPVGTADGLTIKLAGRGYILIDGQRYDIVAVSMNHIMVDLKEVDGKIKKGDEVVII
                     GKQKNSFIDPAHIAKKINLDMEEYLCHINPMLPRIYY"
     gene            complement(23724..24806)
                     /locus_tag="VKN64_06390"
     CDS             complement(23724..24806)
                     /locus_tag="VKN64_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA59903.1"
                     /translation="MNVKDIPVIDNHSHLFDVSDKNREMINILSLSLNEIPEKQLKNS
                     LIYHKYISELKDLLDFEGGYDDVLTKRKGLLETDYKGYVKRLCQDVNMHTFIIDLGYK
                     PASVSLEDFENILPCDVKYMYRIESIVDELWEDKVSFKKAEERFYDEIEKAKSTLDVV
                     AIKSIIGYRTGLNIEKKERSLMKKENYTEKEFRDFFFISALEKAGQLNIPMQVHASFG
                     ESNIDLLKNNPLKLKSIIDDPKYSKTDIVLVHGGYPYSFEAGYLAAMYPNIYLDMSEM
                     VPFVPLGMKKGLRDMIDMCPFNKLMFGSDGFVLPDIHWLAARMAKKEMLNIVKELIEE
                     DYFNEKYGQEILEMVFWKNAKKVYNI"
     gene            complement(24827..26161)
                     /locus_tag="VKN64_06395"
     CDS             complement(24827..26161)
                     /locus_tag="VKN64_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMA59904.1"
                     /translation="MSVKDLIDRDEKVIGTSMKIRLFPLVADESKGSTITDVDGNDYL
                     DFSANWAVANTGYGHPEIVKSVEEQIEKNSFASFTTTITEPSVKLAEDLIKKVPGDFK
                     KKVWFGLSGSDANDCISKLAPIANDRARLISYFGAYHGQTMGSLSLSGHTAQAKFIGS
                     GNVTKIPYPYCYRCPFDKKESECNLECLKFLENQVFTSVSPPEDTSAILLEALQCDGG
                     IVPAPDKYLQELRKICNKHGIYLIFDEVKIGVGRTGKFFGFEKSDVVPDAVVFGKPIA
                     SGMPLSGVIGRSEILDAGVATHLFTTAGNPVSAAAGLATINVVEKEKLMNKAQEVGEF
                     LHDKLQDLKNKYEIIGDIRGRGLVQGVELVKDRKTKEPASRETAKISFRAFQNGLIAP
                     YVGIHSNVLEFTPPLVITKDEISKGIDKLDLAIKEVKEGKITEDDLARYAGW"
     gene            complement(26308..27654)
                     /locus_tag="VKN64_06400"
     CDS             complement(26308..27654)
                     /locus_tag="VKN64_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMA59905.1"
                     /translation="MSSEKNLLEVKPLRNLKFWHIWALGVGSVVGDGIFLLLGEGIQT
                     AGPASIVSFFIAGVLQLFLMLALVELAVGMPSAGAMGPWVKRFFGPWWGFLAGFSWAF
                     AWIIVGGAISLALGRFLSYYIPINELVLAAIAVSIFAILNILGSLIAARAQMWMTFGL
                     VGVMAGLVVFGFPEAIKGIPENFTPFLPEGLESMFLVIPMGAYAFMGTATLTTAGAEC
                     EDVRDLPRALVWASVTFIVLYIGAQFIALGTVPWQELSMAESPFVTAAARIFGSVGGG
                     IINFAAILAASTCLLMGTFYSASRILFSEARKKRMPKFFGKLHPKLRTPIWGIVFIWA
                     ANIAFIIIAKFNTDFVYVTLTMQFTVAVFISMLLSIFSAIKYRKEFPEEVSNLSFNMP
                     LPKLTFTIAIGGTLFTMYYAFKGSPLVIPLSLVWIIPLYIWFKTRPEEAKKADITA"
     gene            28025..>28519
                     /locus_tag="VKN64_06405"
     CDS             28025..>28519
                     /locus_tag="VKN64_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59906.1"
                     /translation="MFKNQMSNQQFKSFIKKNLIEYYDKSNFYDYITSKVNIIHKIYI
                     TDLTAVRTILLDTFQPRGQKPWDPICLFRTYWLICQYGSGSISQWIETLKNPFWAILS
                     GFTPGDIPGIGTLYDFEKRILDFDMGQRSKRLQKKYNFNRKPTQKLKKNQKQKPKKAG
                     IVKNM"
BASE COUNT         8627 a         5757 c         4014 g        10121 t
ORIGIN      
        1 atgtaaaata ttcctcctgt gtataaatag tttttattag tggtgccggg gatcgggctc
       61 gaaccgatac gggaaaataa ttcccaccgg attttaagtc cgatgcgtct gccaattccg
      121 ccaccccggc gggctgcaac catagttgca acattaaaga ttataatata aatgataggt
      181 catgtcaagg ggttagaaaa tttcggttcc tcttcttccg gctcttttat ttctcccgac
      241 ctaaacaagg catattctgt ggcggcagat cccgatattt cagtaaagag gcttgtaaat
      301 aatatgatat taaagatgat taccgctaca tctacttcag tattttgaat atgggcaaaa
      361 tctctttgta caacataggc caaatctaaa gcaacaccga tctggggcat cagcccaaac
      421 ccgatatatt tttgtacact ttgaggtgca ttggccaatc ttgctcctaa agttgcacca
      481 ccgatcttgc cagtaagtcg ggcaactgta tagataatag caattatcat aaaggaactg
      541 gtaaatatat tggtaatctg tatcttagta ccagccaata taaaaaatat ggcataaaga
      601 ggcattgtca gggtatctat taaatctgca accccgatat gtttctctgc aaagttacaa
      661 aaagcaaagc ccacaaacag attatagatt aaaggagata agtgcagtgc gatagaaagg
      721 gctgaacccc ctacaatggt taataagatt aaaaatacct tttgtttttt tgaatgatca
      781 aaacatatgt agcgctgggt taaataacca aaaagaagcc ccaaaccagt tgctgagagt
      841 atttcaactg tcggtataat aaacccgttc aaaacattta aagtctgatc ggggtcaatt
      901 gatatcaaag aaattggttc aacaaaacta aaaatcacta acgctaaggc ctcatctaag
      961 gccaatacaa tcattaatgt agccgaaaat tctccctgca ccccatattc ccttaagatc
     1021 aagacggtcg ctgccggagc agttgcagta gcaaaggccc ccaataaaat agctaaatag
     1081 aggggtttac caatatataa catgctcatc gtaactaata caaaggcacc gattgactca
     1141 agtaaaataa tcaaggtaaa ttcggactca agttttttaa gtacctttaa agaaagtgaa
     1201 gtaccgatcg taaaggaaat aaaggaaaca gctaaaattg taacaaagtt aaaggaattg
     1261 atcagatatg tataccactc tgtaaattca gtactaaaaa aggggatatg ttcaactaca
     1321 ctggcactaa atattgcccc tattattaca taaccggcta cactgggcaa ccggtacttt
     1381 tcagtaaagg tatggattaa gaaagtaaaa agtaataata ggccaataat aaatattact
     1441 ttgtagcttt tatgatgaac catcatccaa tgttgaattt caagtaggtc atgtgtataa
     1501 tccatttaaa acatcctttt taagtatttt cttctaatat ccataagtaa tcatagggtt
     1561 ttagctgata atccttaaaa cttttctctt cttctgttag attattgaca attgttaatt
     1621 ttttatcttc ggcctgcctc tttgagataa gtagattacc tttagtctca tattctactt
     1681 cagatttaaa gagcttcaga ttctcatttt ttagtcttat catttctttt aggccccgat
     1741 aaataaggcc ggggtcactt tcgggctgct ttaaagcggt agtttttttc tcctgattaa
     1801 aaggccctct gtttaaataa cggctgtcct tatatccata cttttcattc atcttattat
     1861 agaaatctag gtcattttcc atgcctattt catccccaaa gtagataata ggggtacctt
     1921 taatagaaaa caggattgaa taggctaaaa ggaccttgct gacatcttta tcaagaaggt
     1981 catatagtcg ccctgagatg ccttctccct cacgaaaggt ccatctttca tcttttagat
     2041 aatgttcatt catcaattct ctttctttgg cagtcacaaa ttccaaggtt aattcatcat
     2101 gacacctcaa gaaagtgaac caggcggtcc cgggcggttc ctctaactta ccaatgatct
     2161 caagctgttt ttctaaatag tcactttctt gttctgcgat ggaaagaaaa attctgggca
     2221 ttatcggaaa attatatact acatgacatt catcaccttc acctagatac tctattacat
     2281 cggacggcat ctgattggct tccgctatca tgacagtgcc ctttgctata taatcaaagg
     2341 cagctctgaa tatttttaat atcttatggg tctcttctaa attttcacag tccgtaccct
     2401 cttttttcca taaaaaggga gcagcatcca tgcgaaaacc atctaccccc ttggctctcc
     2461 aataggtaaa ggcctttaac atctcataaa gaacggctgg atttttataa tttaaatcgg
     2521 gttgaatatc ataaaatcta tggaagtaat aatcgtcagt tgcttcattg taggtccagt
     2581 tactattaga gatccctttt aaaagcaggc gggtttcagt atatttttgt ttgtcttcat
     2641 tccagatata gaagttgcgg taacgggcct cttttgattg tcgggcatct ttaaaccatt
     2701 catgttggtc ggaagaatga ttaaccgcta tatcaaatac tatttttata tttttagcat
     2761 gggctttttc taagaaatca aaaaactgct ggttagtacc gagttcttct ctaatctgat
     2821 agtaatcaga aatatcaaag ccctgatcta ccattgggga ttccaaaatt ggcagaagcc
     2881 agagggtatt tacccctaat tcatctaaat agtccaattt atcaattaaa ccttgaaaat
     2941 catctgcaaa aagatctaca taaagtgagt aaactatccc ttcttgatgc caattttcat
     3001 tgttcggctg atagtccact tcatctttac gtttatctaa aagttctttt agcttattta
     3061 aatattcttt ttcttcccca taaagttgct gccataattt ttctatcttt gttaacattt
     3121 cttccacctc aattaagact ctattattag cccgtgtaat atatcataat cacttaccct
     3181 taatttaagg gttctaagag ttttcatgat ctcatttaag attacgatcc ccgcgattat
     3241 aatatcagcc cggtctggat tcagaccttt gacttttttt cttttttcta aatcaagttt
     3301 tcttaaccta tctaatactt tattaatatc gggataattt aaaatataat catgaatggc
     3361 ttgttgatca tattcttcta attttaataa gatagctgcc agagttgtaa ttgtgccgcc
     3421 aacccctatt aaattttgca ctcccggcaa aatatccagc tctttttcta actcttgctc
     3481 aacatattcg gcgatatttt ttatatcact tctgctccac tcctgatgag gattatcaat
     3541 aaatctttct gttaatctaa cagccccgat atttaaacta tccatttttg ttttcttatc
     3601 ttcttcactt ttccagataa attcagtact tcccccaccg atatctataa ttaaataatc
     3661 tgaataatta tatccatagg ttacaccttt gagaataagt tcagcttctt tatggccgga
     3721 aataatttct aagtcataac cgatctcttc tttaattaac ttctgaaact tatcagcatt
     3781 atttacatcc cttaaggcgc ttgtcccgac cagtttgaca gaagatacct catattggtc
     3841 tatttccctt tgtaaggatt taagggcttt caggcccctt tttatcgcat tttcacttaa
     3901 ggtcttatta ctatctacac cttcacctaa ccgggttgta attaaatcct ttttaagttc
     3961 ttttatcttt tgttcttgct gctcggcaat taacagccgg gtcgtatttg ttcccatctc
     4021 tataactgct ttactcatct ttttttaact cctcacaaat tttgcatttg tccggccaac
     4081 ctgttttatc aaatactatc ttaccaaccg gattctcttt attaaccaaa taatcggctg
     4141 tatgggtatg taagcatttt atgccttttt tctctttgat accgcctaca ccggaatgtt
     4201 ttaaggtata tatgatatct tccgatattt ccttaatttg atctatttct tcttggctca
     4261 acatcgccat ccttatttga gcataattcg catgtgcttt atctaatttt tctattaaag
     4321 cattatcatt atttacttta tcttgtattt tttctatcca actttgatct tcaagcttag
     4381 atatctcttt atttaaataa ggacaggtca accaatatgt agtaggaaaa accctcttat
     4441 taaaaaaagg gtacactttg actacagccg ggtacccaaa agggcagttt atagatacct
     4501 caaccaggcc ggctggttct ctttcaatct gtttttttat tatatgataa gtttcacttt
     4561 tctttaaagc cataaatacc taccctattt tatatatttt cactttggat tataccataa
     4621 tttcataaaa tcgcaaaata tacaaagaaa aaaccccaca aatttgtggg gtattggttg
     4681 ttttaagtta tgacaaaaat aaaaacaccc ccacatgggg atgttcaatt acaaactata
     4741 tatataaatt aaattaatta atttcggccg ctatcacttc gcttctcgac tctctttttt
     4801 aaatcgcttt gacgctcact gctttgttgt aaaaactcat ccattttttc ttcaaaagac
     4861 ttgttaggag catggtcgat tctatcagaa ggatccttta actgtttgat tgaaagacca
     4921 attttaccat catcgtcaat gttgataacc ttaactttca cttcttcgcc ttcttcaaga
     4981 aaattgccaa tatccttcac ataggtattt gctatttcag agatatgaac tagacctgtt
     5041 tcaccatttg gtaattcaat aaaagctcca aaatttgtaa tacctgttac ttcaccttta
     5101 actgtacttc caacttcaac ggacatattt aaaaaaattc ctccttgata tttaatgtaa
     5161 actaattata ttacagatgc acagccgtgt caaatgatat tacgacaaaa ctatagcttt
     5221 ctagacaaag aaatacgttt tcttttctca tctatatcaa tgattttaac ccttacggta
     5281 tcacccactg aaattacatt taatggatga tctacatatt tatagcttag ttctgaaata
     5341 tggataagcc catcctgttt aaccccgata tccacaaagg caccaaaatc taccacattt
     5401 ctgactgtcc ctttcaaaac catacctact tctagatctt caaactttaa aacttcattt
     5461 ttaaagatag gatctggtaa ctcatctcta gggtctaagc ccggtttttt aaggttttta
     5521 attatatcgt ctaaagtaaa ttcacctatc tcactttgac tgatcacttc ttttttattt
     5581 aaatcattta tatcttccct cagttgtttt aatttatctt ccgcgagaat atcagccggt
     5641 tggtacccta atttttctaa aatcaattca gcctcttcat aactctcggg gtggataaag
     5701 gtataggcaa aagggtcttt tttactgaaa aactgtaaga acccagaagc ctgttcaaat
     5761 gttttggctc ctacaccata tacatcttta atttctttcc tctttttaaa tttaccattt
     5821 tcctgcctat aattaacaat tttatctgct acagaactat ttattcccga aacatactgt
     5881 aagagggagg cagaagccgt atttaaattt accccaacct gattaacaac cgattcaact
     5941 acctggtcta aggattcctt taattcagtt tggtttacat catgctggta ctgtccaact
     6001 ccgatggagc ggggatcaat ttttaccagt tctgaaagcg ggtcctgcag tcttctggca
     6061 atagatatgg cacctctcac actcacatcc agatctggaa actccttaat tgcttcttca
     6121 gaagcagaat agacagaagc acctgcttca tttacaatga tatatttgaa atctatatct
     6181 cctcccatct cggtcacaaa catctctgtt tcgcgtgaag ctgtaccatt gccgattgca
     6241 atcgtgtcta tattatattc atttaaaagg tttttaacct tttgagctgc cttttctttt
     6301 tggttttggg gtgggtgagg gaaaatcgtt ttatattcta aaaagttacc catttcatct
     6361 atggcagctg ctttacaacc cgtacggaaa ccgggatcaa tacctaagat tttatgaccc
     6421 ttatagggag gtgtcaacag gagagatctt aagttgtctt tgaaaatctc gattgccccg
     6481 gcctgggccc gttcggttaa aagatttcta agttcccggt ttaaagaagg ttcgattagt
     6541 cttttaaaag agtctttgat tgtatcatct aatatttcag actcaactgc catttcttct
     6601 gataagatag tatctttaat tatctctaac atcttttctt catcatgttc aatacttacc
     6661 tttaagactt catcactttc tccccggtca atcgctaaca ctctatgatc aggtatctta
     6721 gcaatttttt ctttatagtc ataatatagt tcatatttat ttttttcatc ttcaatatct
     6781 tcatcttttt gagatgtgat taaacttctt tctttcatct cttctcttaa atttttccgt
     6841 accacattat tattcgatac catgtcggcg ataatatcag aagcaccctg aatagcatct
     6901 tcaacatttt ctacttcttt ttcttcatcg ataaattctt ctgccttctg gttaagttta
     6961 tttttttcat aaccggctaa gatatattct gctaatggtt ttaaaccgcg gtcagtggct
     7021 ttagtagccc tggtctctct tttttcttta aagggcctat agatatcctc aacttcctga
     7081 agggtttccg cctgctctaa tttttcttca atttcttccg ttaatttttc ttttttagat
     7141 aaagtatttt ttatttcttc tttacgttca aataagtggg ttaaatattc atattcctca
     7201 ttgacatctc taatctgggt ttcatccaga ccaccggtct gttcttttct atatctcgca
     7261 ataaaaggaa ttgtattacc atcttcaatt aagtctatta ctgatttaac cgacttttgt
     7321 tttatctcta atttatctga tatttttttt gcaattttac ccatcaatta tcatcctgtt
     7381 cttctgaatt ttgttgttgt gtgttttctt cttgattatt atcttgttct tcctcaacag
     7441 gtattaaaag aatttcaccc ggtttaacca agcccagttc ttcccgggca actttttcaa
     7501 catattcagg gcttctaacc ctctctattt cttcttttaa ctccttgttt tcttctattt
     7561 ggttttgtac ctgctgctgt aagctattta atctatcttc catctggttt accttacgga
     7621 tattcatcat cagtttaaag gcaaagacca caatcacaat gattaaaaga tagaccatag
     7681 cttttttaaa aaaagtttca aaactcattt tatctgctcc ttaatttatc tatcaaaata
     7741 tatgttttta ccggaagcgg aaatggggac accgaccact atctctcctt caattagacc
     7801 catcttttta gcagctaccc caattctata cataatccta ttatctacat ttaataggct
     7861 ggctgtttta acggctgatc ctaaggcgat tcctaagtcc attaatcgcc aggcacagat
     7921 cggaccatcg aactctggtc ctgattttaa cttcagttct tcacatttat caaaaccaca
     7981 ggctccacaa tcaagaccta taacagccgc ctcattaagg gaaagtagaa gtacctgttc
     8041 actatttctt acattttcgc catctctatc aaagtttcct tttccactct ccttaccata
     8101 gtttatcatt tcatctgcaa gtttttcaat cttatctccg ccgattacct taatttctac
     8161 ataatcctga ccagcggttt ttggtgcggt tctagctgaa acagccatta attcagccgc
     8221 agtttccatt atatccatat atttttaccc ccttataatg attaaagcag ccggttttac
     8281 ccggctgcaa gtaattttta agatgtttat tttctaaaat attgcttctt cggtttcatt
     8341 atcaaagata tgcatcttct tagtatatac acctaatttg attttatcgc caacttcagc
     8401 atcactttct gcatctaccc tagcaatcat ttcatgctca tcaactgcga gatagagata
     8461 tatttcagaa cccatcggct ctacaacctc tacttcagct tcaaaagagt tttcaggtgt
     8521 tatatctaga tcatgtgtaa ttctggcatc tgttaaatct tctggtctaa ttccaaaaac
     8581 aactcttttg ccagcataat ctttgatcct atctgtaaat tgctccggaa ctttaattct
     8641 aaaggcacca aagccatctg caaagtattc gtcaccatct ttttctattt tagcatccat
     8701 gaagttcata gcgggactac cgataaagcc ggctacaaac atattattgg gatggttata
     8761 tagttctaac ggtgaatcta cctgctggat atagccgtct ttaaggacaa cgattctatc
     8821 tcccattgtc atcgcttcag tttgatcgtg agttacatag ataacagtag tttttagtct
     8881 atcatgtaac ttacttaact ctgtcctcat ctgtacacgt aacttagcat ctaagttaga
     8941 aagaggctca tccatcaaga ataccttagg ttctcttaca atagctctac ctagagcaac
     9001 acgctgtctc tgaccacctg ataactgttt gggtttacgg tctaagaggg gctcaatact
     9061 taaaatttca gctgcttctt ggactcttct ctctatctca tctttgggga attttcttaa
     9121 ttttaatcca aaagccatat tatcatatac atccatatgg ggataaaggg cataattttg
     9181 gaataccata gcgatatctc tatccttggg tggaatgtca tttactactg tatctcctat
     9241 tttaatctta ccttctgtaa tttcttctaa tccggcaatc attcttaagt cagtcgactt
     9301 accgcaaccg gacggaccaa ccagaactaa aaactcctta tctttaactt ctaagtcctg
     9361 atcctttgcc gcaacaacat caccaaatct ttttgtaaca ttttcaaaag taacactagc
     9421 cattaattaa ttcctccctt tattaatttt tgtggattcg catttcttta attcgggttt
     9481 taaaacttta atttcttctt cggcctcttc attatttatt aaatttatta atacctctgc
     9541 agccatgcgg ccggcctgac tgagaggttc tttaactctt gtcagttctg gttctactat
     9601 cgaatttata atagtgtctc cataagatac aaccggataa tcctggggga taaagtagcc
     9661 gcccattttt atagcttcta ctaaaccgac tgaaagtaac tcatcagtta caaaaaaact
     9721 atccggcggg gtctgtaaat caaccaggtt taaaaaggct tgatagcctg cttctctggt
     9781 tccgtccgtc tcaattacta aatttgaatt aaaattcaag ccgtggtcta caagggcatt
     9841 tttataacct tttatctttt cgctttcaat atagtcatta ctattacccg taatcagggc
     9901 tatattttca tatcctaaat ctaatagatg gtttgtggca agataggccc cctggcttga
     9961 atctataaga atagtaggaa ttaaaatctc ctcacatagt ctattaacta gtacaatatt
    10021 atcatggccc cttaacttag tattaactat gtgttgatca gcaatttcgc ctcccaagat
    10081 gattgctcca tctacccttt tttcttctaa taaatctaaa tatgattttt ccttttctag
    10141 atcattatca gtattacata agattatttg ataaccagct gcttctgcta ccgattctat
    10201 attttcaatg atctgagaat aattgccctg agaaaggccc ggtagtaata aggccagaat
    10261 atgggtttcc tgtttggcca gtccctgagc taatttatta ggtttaaaat tatattctct
    10321 cgcgattttt aaaatctctt ttcttgtttt ttctcccacg cccggtttac cattaatagc
    10381 tctcgaaacc gttgactcgg ccactccaac catctctgct atatccttta aggtcaacgg
    10441 caaagttaaa acctcctttt tacgcaaggg cttgcgcaac tctatataat tatttcaaca
    10501 taaaaagcaa aaatccttct taattttaaa aaaattattt agaaatatac caattaaggt
    10561 tgcgagaaac ttcttttatg gagagattgt caaagatgga gttgaattgt tttaagccgt
    10621 tttctatata gttaaaatat ccgccgcctt ctaatttttc aattatatcg acgtaaaatt
    10681 tacgttgaaa cttataatcc tggattaaaa ccacattttt agcgattctt ttggtttcgt
    10741 taaaaaactt ttggcgggtc ttttcatcaa gtccatgtgc taccatggct gaaattacca
    10801 tgtcaaagga gttatctgaa taagaaaggc cttcattgac atcacctttt tcacagtaaa
    10861 gatcattatt tatgcccctt tttaccattt ccggggccac atctatcccc gtgacagaat
    10921 agcccttttc ctgaaataca taaccaaagg ctccggttcc acatcctata tccaaaatgg
    10981 tatctatctt ttcatctttt attagactaa tattttcatc tactatattg ccgtaggacc
    11041 agacctggta tttaaagaaa agttggtagt aaggggctat attattaaac aaccggccat
    11101 gttcatccat atcgtaacca cctattcttt ttaagaccct attatctcat ctaagtctac
    11161 atctttcaca ctatcaaagc gataatgagc atgacctacc cgagagtcgg ggccaattcc
    11221 cactgcagtc atgttagctg ctagcgcggc atctataccg gcctcggcat cttcaaatac
    11281 tacacattcg gccggcttca aatccatctt ttcggcagta tgaaggaaaa gatcaggggc
    11341 cggtttcgaa ttttttacac tatagccatc agaaattgta tcaaacatat cttttatatc
    11401 taacttaccc actactatct ttgtattttt actggcggag gcaatcgcca tcttatagcc
    11461 atcttttttg actcttttta atatctcttc gatcccttct aataaattat cttgcgagat
    11521 actttttaga tactctctgt aataattatt ttttctttct gctagttctt ttctttcttc
    11581 tggagttacc tttctatcat tggcctcaag taataagtca aggcattcca tacgggaaac
    11641 acctctaaat ttttcattaa attcgcggtc aaaggctaag ccctcctcct cggccaactt
    11701 cttccagcct aaataatggt attcggctgt atcggtaatg acaccgtcta gatcaaatat
    11761 aaatcctttt atttcagcca ttattgtcta cctccctata attggaattg ttttaaacac
    11821 ttgatatctt cgtctaaggt tggtcgccaa atttcaatca cttccgcctt acattctctg
    11881 ggggtatgga ttccttcaaa tctgtaacaa aggttctgaa aggcttgatc aaagttctgt
    11941 ctttcttcag taaaaacagt gctggctaag gaaatatgga atttccaatc ctcataattg
    12001 gctattgttg aaatattttt ttcagctaat ttgtcatgta gctcattact aaactggacc
    12061 aactgcttat tttcttccac atttaaaacc aaaaaattat tctccttaaa agaataacag
    12121 tcgaagtttt taaccaaaat atctatcttt gcatacttta aggtgagaga attaatgatc
    12181 tcaatagctt cctcaactct atccttatat atacggtcta aagtaatgtg aatctcaggg
    12241 tacatgtcat ctggataaag gtcaaattgg gaatggagct tatcctgtag gtattgagct
    12301 aagtctagta catctccctc cggttttaat ataataaagt attcgtcttt aaaagaagaa
    12361 tttttaatca tatagaatct cctcatattt tgagttaatg aaattacaga gttttttatt
    12421 actgtctatt tcattaacat tgttattttc tttaatatcc ttatataatt cagtaaactt
    12481 accttctgta aagtcccttt tttgcagatc gttgttcatc ataaataaaa tatctttatc
    12541 attgaaatct gctaatacct ttatattatc caaattaccc caaccaaatg gagtatcaga
    12601 aatgataatc aggtctgctt tctgcatctt ttctttattt tcttggactg ccctttcatc
    12661 tatcggggca aagggcggaa tttcaaccac ttcaaaatta aatctctttg ccgattccca
    12721 atcagagtcc ccgcggttta aaacccctgt gctaacccca atattgaggt catttaaaat
    12781 attcatgata tcattgccgg ttccaccccc acagatgaga tgtactttaa aatcatgggc
    12841 tcgcatctgc tgactgcctt tttgccgggg gataatgatt acatagggtc tctctgtaat
    12901 aggattatat ttgatatcaa catcagtgtt gtacacttca ctgatatttg ccttagtaat
    12961 tacttcttct acttctccca tactgtaaat cttgcctttg tgtaagagaa ttaacctctc
    13021 acagtactgg ctgatcaaat tcagatcatg ggaaaccgct aaaacggcca tctctaattc
    13081 ttcatttaaa tcagagatca aatcaaagat atttctctgg taatttatat ccaaactggc
    13141 ggttggttca tccaaaagta aaagttccgg ctcctggaca attgctcggg caatgattac
    13201 ccgctgtctt tccccaccac ttaagttctt aatagattta tccttaaact tggctgtatc
    13261 ggttaattgt aaggcttctt ccactttctt tttgtcttcc ttacttaccc gcccccagcg
    13321 gtcctgatag gggtttctac ccatcataac gagatcatag acagtaaaat taaagctgac
    13381 atttgtatcc tgggggacca ctgccatctt cttggccagt tcttgatatg aataatcatt
    13441 taaaagattt ttatcaagat aaatatagcc ttcatcagga tccagtaact tactgatatt
    13501 cttaagtaac gtagacttac cggaaccatt cggcccgata ataccaataa actcatgctc
    13561 ttttaaatga aaattaattt catgtagtat tttctgtttt gcataggaaa aattaatatt
    13621 ttctagcttt agcaatcttc atcaccatat ttcttttttc tgttttctta gtaaatatat
    13681 aaagtaaggg ccgccggtaa gggctgtaat tattccgacc ggtatctcca tgggagctag
    13741 taagatgcgg gctaaatcat cacttacaat taagaatatc ccgcctaaga ttgcagctgt
    13801 tggaaacagc tttttatggt caggtccaac cagcattctg gcaatatgag gtacaattaa
    13861 accaataaaa ccgatactac cgctcactga aacaacagaa gcagtaacca gggaagcccc
    13921 gattatcaga taccttttgc ttttttcaac atctacacct aaatgctggg catccctttc
    13981 ccctaataat atcaaattta gatcttttaa aaagaaaatt attatgaaat tgcctactac
    14041 aaaatagggt aacattaacc ttatatcttg ccagctagca gttgataaac tacccaataa
    14101 ccagtaaaca atcttactta agtccctggt ccctataacc ataaaaaaag acatgaccgc
    14161 atttaagaca aaaccgaccg ctaccccggc taaaagaaag gtaacaaccg gtaccttatc
    14221 tttggttttc gctaagttat aaacaatata aaccgtgata agggaaccaa aaaaggccat
    14281 cagtggtatt gtattaaaga aaagaaaact tatctgcaaa ttcaaaacga tagccagggt
    14341 gacacctgta ccggccccgg ccgaaatacc aacgatatag gggtctacca tcgggtttct
    14401 gataacaccc tgaaagacta ctccagcaat agaaagaccg gctcccacta aaaaactcaa
    14461 tataatcctc ggtaatctga tctgctttat tattacattt aaggactgtt ttacatcttc
    14521 cccgaagaat attattttta aaactttaag aggggaaaga ttaattgaac caaaacttaa
    14581 tgatattatc cctactacaa atagtaaggc aattattgcc aatattatgt ataaataatt
    14641 tgtctttttc tccataaagt atgcctccac tattaatact taacatatac tataacacaa
    14701 atttgtcatt atatccacat tttagagagt acaagaggac atcctaacgg atgtccttta
    14761 tgggtggatt aatatttaat tttttgagaa tcttcgatct catatacttg attattaacc
    14821 tggattggta ttatttcctt aatattgata gcttctattt taatattttc ctgggtaata
    14881 gaaaccttgt atttattctg ttttacatta atattaaaac taaccatatt ccatctattt
    14941 ggtaagtgag gatcgatttg aagaacgtct ccttttaatc taagtccagc aaatccaaat
    15001 acaacagact gccataatgc cccgagagaa gcggcatgta agccggcatc actacttgcc
    15061 atatttctgc ctagatcaat tcgggcagcc ctctggaaaa gattatatgc cttatcaatt
    15121 tcacctaatt tagcggcaac tgcagcgtga attcctggac ttaaagaaga atcatgtagg
    15181 gttaggggtt cataataatc aaagttggct ttaatgattt cctcatcaaa tacttcttct
    15241 aaaagatggg ttaacataac tacatcagcc tgtttagaaa cttttgactc agtaatctct
    15301 tcccaaccta agtcatcaaa tatatctccc acatccccct gcttatactt tgttaagtct
    15361 atttctttta aatcttgaaa cccttcaaat tggggaataa tgttattatc atcggacttt
    15421 ggaataaaga tattatctcg tttgttagcc cagtctttta tttcttcttc ttttaagtta
    15481 agctcgtctt ttatttcatt tagataagct tcttcttctt tttttagata ttttatatag
    15541 gcaagggcct tttcaaactg ccagtgaacc atataattga taaaagcatt attatcgata
    15601 tgttctttgt attcatccgg gcccatcaca ttattgattt catatctatc ctggccttga
    15661 ttgtattcca aacgggaagc ccaaaaccta gtacttgcaa ggaaaacttc aaaaccatat
    15721 tctttcatga attcaatatc tccggtcgcc tggaaatatt gatcaaaagc atattgaata
    15781 tcaacagtga tatgctgttc aatttctcca caccagattc taatttcctc tcccgttagg
    15841 atatctaccg ctccatattt aggtgtggtc tcctggccgg tttcagcact ttcccaggca
    15901 aacatagcac cttgataacc gttattttgc gctttttgac gagctcccgc aagtgtatga
    15961 taccgatatt ttaatagatt tctggctact tcgggatggg tgtagatgaa gaaaggtaaa
    16021 atgaatactt cagtatccca aaatacatga cctttatatc cttccccact taagcctttg
    16081 gcggcaatac ttatcctgtc gtcatggaca gggatcatct ggattaaatg aaatatagca
    16141 aaacgtaggg ctaattggtc aaaatcacta cctgaaattt tgaagtcgct ttcttcccag
    16201 actgaacgcc atttctgtaa gtggttttcc tttaattctt tataagctgt atctctaata
    16261 tttattaaat tatctatcat tttatcttct aaatcttcat cttctcgaaa atctttatcc
    16321 ttattagtaa aagcgcttaa tcttttaatt atggtggctt tttctttttg ttttaagtta
    16381 aattcatata cccttttaat ccttctgcgc tccgctttaa aatctttttg gggttcaaat
    16441 ttctctttgt ttttataaaa atcatggtgt aaaccaaagc tcatctttat ttcagactgc
    16501 tgggtttcag gctgatatac gatataatct tcaattaatc ttttttcacc ttcttttagg
    16561 tgttgagtcc cactattgct aacccgtccg tcaaagcctg aaataagttt aatctctccc
    16621 gaaaagttta acggttttat ctcaatctta gaaacaccgg tgtgtagatt atttagcgaa
    16681 ataaatcttt caaaggaaaa ttcgaccatc tcaccatttg gactttccca atggaaatct
    16741 cggataagca aaccgtcttt taaatttagt ttgcgactat attcgaatat ttgtccctta
    16801 tctaatttaa atagttcacc attaatgtaa atttcagttc taatccagtc cggcaagttc
    16861 ggcatttccg ttacttcacc ggaaaattca tcaaatatac cggcaatata gaaaccgcgt
    16921 ctttccttag gatatgtttc ttctgcactg gctctaagtc ccatatatcc atttcctaga
    16981 ctaaaaattg tttcaaattt cgcttgattt tcttttttaa aggctgtttc ttcaataatc
    17041 cagtttttat aaaaatcttc accagcttca taggaaatag acattataac accccaatta
    17101 atatttaaaa tatagttttt gcaattttca cactatttaa tataaaaaat ggagcggaaa
    17161 acgggactcg aacccgcgac cttctcgttg gcaacgagat gctctaccag ctgagctatt
    17221 tccgcctggt ttatataaaa tatataatgg tggaggggag aggatttgaa cctctgtagg
    17281 catcgccggc agatttacag tctgctccct ttagccactc gggcacccct ccaaatatgt
    17341 gttatttaat ttaaaaacgc tttaaaactt aatatgtcag cgtttcaatc cttatttata
    17401 gtaatatatt ttagcctatt tgtcaagagt aaaatccaaa ttgcccctta aaaatacctg
    17461 caatttgaga gggtggggtt aagctattaa acttaacccc ggttcattta tttagaacag
    17521 tccagttatc ttaccatttt catcgatatc tatatcttca gcggaaggtc tcttcggtaa
    17581 accgggcatc ttaagtaccg gtccggtcga aggtactaaa aagccggccc cggctgaaag
    17641 ggttacctgg gagactgaga ccgtaaagtc agtcggtctt cccttaatct ctggcttatc
    17701 agatatggag ttttaggtct tggccataca gatcggtaga tcaccataac ctgtttttca
    17761 aaggtactga tctgtttctc ggccgccgaa ctgtaatcta ctccatccgc tccatagatc
    17821 actttagcga tcttgtcgat cttttctttg atggaaaact cttcactgtt cttcactgta
    17881 tagatactca aaataaatct ccccgtaatc ataaaatatc tgtgctcttc ttgcctagtc
    17941 catgataaat tcatgatctt atattatgtg ttctttatca aattgagtaa ggctagtttt
    18001 tttaattctt ctgctttatt ttgtacttta acctaaatac ttattccata tattactact
    18061 tcgtcacttg cattttcgat ttcctcaatc ttattttcag gtgcattatt ggtaatgctg
    18121 attttcacat cattatttat aattttgaca agataagtag ttactactcc attttttctg
    18181 tgagataatc aattaaggct ttttcataat tcccttttaa tacaatcaga aaaatataaa
    18241 tagataaatc aaaattttat ctaataatat gaatattttt aaatatattt atttacaact
    18301 tatttcatct aaattaaaat ctacttaccc tagaaactgt tgtaatattt ttaaaaaaat
    18361 taaattagac tcccttttct tttccttgat tatttaatct ttctaacgta atcttggcag
    18421 tgattaaagt gttacagttt aataaaatat ggttttttac tttcttttta cccttgactc
    18481 tgatattttt caaacttaga ttttctttta accttgtaaa gttacgttct acgctagtcc
    18541 ttttataata tatcttctgt caggttttag atctacgata tcatgacaaa atatatctga
    18601 gataaatttt gggtctcgtc ttagttacat gtccataatt agaattagaa caccaggcag
    18661 atccatggac acaatttact ttactgcagg catcaagaca tctaaattta ttaacttcat
    18721 tgtaataacc tcagtaaacc actttatggt cagcagttca gcagggaatg caatcaaaat
    18781 cgtgattcag cgggaggctg tttagcattt cttttgttga taggtacaca ggctgttcat
    18841 tgtaattaaa tagtatattt tatagatact attaaaataa taatacgagt ccatggtccg
    18901 atatttagtc tgttcacatt taattcttca cttacctttt ttatgtctga ttacatttag
    18961 tttcattatt cgtggaaaat taacagcaat tgtaagttca cttttagtat caacggtcag
    19021 gtatactttc agttgaacca ggatatcttg tttttttaag ctatcaaact tagttcccca
    19081 atttatatta ccacaatcgt tttcatcaaa atgagatttt ggtatagctc gttcatatga
    19141 ttttaatttg gaagcatcaa tagctgtttt ttcagagtta attaggtcta atggatttgc
    19201 tttatccaat aattgaaaat ttttttctaa acaatcagac tttactaatg tttcaaatta
    19261 ataggacaag gtaacttaac ttggcacttt accgtaaaat ccaaaaccaa agacataact
    19321 gaaggcagaa tagctcttaa acctgcgaac atggtcagct ctataggcat attttcaacc
    19381 ccttgagcca aaaatacact aataattggt tggatagagt attaattgga ccccttttta
    19441 aattattcga caacttatta gtggtttgaa taagtcaata ttttcaaaaa tcaattctaa
    19501 tcttgttttt ctttgatgtt tgaataaatc ttctaaggaa aataagctca tttgtcgaat
    19561 ataggtaata gtttagcttc tttttgcgtg tatttttatt tgctattaaa tatattcaac
    19621 aaaaataaag gtgaaacctt ttttacgccc caataattac tgtcatttca aggcttattt
    19681 ttatgaaact tgcttattta atgaaaaata atatctacaa aggaaaatcc cctttcagta
    19741 aacagttttt ttatttaatc tgtctaccta aggggatgca aattaaaaaa ggggctcctt
    19801 ttataattag ctcgaaatct taaagttatt aatccttaaa aaattccaac ctaactagga
    19861 gttttaatta tttattttta ataagtaatt ttaaataaac ttattgtcct aattacaaag
    19921 taataactat gttcttatat tatttagtta atattattat atttaattga ttatatcttt
    19981 tttaacagat taatagacag ataacttaaa attttagatg tttttaataa taaattctca
    20041 tcaaagtcaa aagaaggagt atgtaaatca tttgtataac cttttttttc atcaaaacta
    20101 ccatatctaa tataaacaac tggtatcctt tcagaaaaaa atgcacaatc atctgatcct
    20161 aatgaaggtt catctatttt tactaaatct ttatcttgta aaatcttcat tagtatattt
    20221 tctgtatttt ttgttaaatt ttcatcacaa taaagtggtg gctctccagg aactaattca
    20281 aaattatatt taatattata tgacatttta agtccctcta atagcttttc tattttctct
    20341 atagctatat ctcttgtctt cttatctata cttctaattg taccttttaa agtaattttt
    20401 ttaggtacaa cattatgtcc accacctcca gatatctcgc cgacattaat cacaagtggt
    20461 ttcacaggat cagttatgcg agaaacaata gtctgtaatt caactataaa taacgaggaa
    20521 gttacaatag tatcctgggt actatcagga tgtgccccgt gtccaccttc ccccattata
    20581 tctattgtga attcatcatt tctggctgtt aaagcaccat atctgattcc tattttacca
    20641 ttttttattc ctggaaaaat gtggccggaa atcaaagcat ctactttagg attttctaat
    20701 actccctctt caatcataat ctttgctccg ccaggatatt cttctcctgg ttgaaatatg
    20761 aattttatag atccttcaag taaatctcta tgttcactta aaatttttgc agctccatag
    20821 accaaagttg catgtccatc atgtccacaa gcgtgcatct tacccttgtt ttgagaagca
    20881 aatggtaatc ctgtttcttc ttcaataggt agggcatcta tatcagccct tattgctaat
    20941 actggttttt tcttcttccc ctttaataac gctgtaatac ctgtattagc tataccatgt
    21001 tttatatcta aattcaatcc ttttaatttt tctaataata atttagatgt ttcataacaa
    21061 tctagttcta gttctggatt tttatgaatt tctcttctca gctcaattaa ttcctgagaa
    21121 atctcttcac ttgtagcaaa tagttttctt tctaactgat ccacttctat atcctcctat
    21181 tatgcctttt ttcaataaat atattcccta ttaattttat atttaaaata tctctacata
    21241 tttatctttt tcccaggaag taacatgatt catgaaatct gaccagtctt gccgtttcat
    21301 ttttaagtat tcactgatca tagtttcacc aaatacctcc ctaaaaagtt tatcttcttc
    21361 aagggcatcg agagctctac ttaaatcccg tggtagagag ctaacgtttt tagtttcttt
    21421 ttcttcttca ctcaaattac taggatcaat ttctacggat tcccccggat ctattttgtt
    21481 tttgatacca tctaaaccag cagttaaaac tgctgctagg gctaaatagg gattactaga
    21541 aggatcattt ggcttaaatt ctattctagt actatcttta gactgcatct gacgattgaa
    21601 atctgtagaa ggaattctta ctgccgcctc tctattatta tggccataac aaacataagc
    21661 agaagcccaa ttatgtggtt gtaatctttt atatgaagtt atagttgatg cagtaaatgc
    21721 acaaactgct tttatatgct ttaaaactcc acctataaaa taatatgccg tttcacttaa
    21781 agcatgtgca tcattttcat cataaaatat attctctcca tttttccata aacttagatt
    21841 tatatgtgct ccgctacctg caagaccatt aaatggtttt ggcataaaag tagaaatcaa
    21901 gtcatgtttt tctgcaatgc ctcttattgt atctctgaaa aatatctgtt tatcagccat
    21961 atttaaaatc ttatcatatt ttaaacagta ttcttgttga cccggaccat attcaggatg
    22021 atatttttca atttcaatat tctgtttatc taatgcttct aatacatcta aagtgatttc
    22081 attcatttca ttcatgcctt tggtagtata acaaatacta ttatcagctg gttgcaattc
    22141 tccttgttca tcatgcttta gaaaatagaa ttcattctca aatgctgcat atggttcaaa
    22201 ctcactgtcc tcaataatat tttttaatat tgttctagca catactccac tatcttcaag
    22261 agtttgctct ttaatatcac aaattaaagc tgcatttttt ctcgcatatg gtaatacttt
    22321 aaaagtatca agatcaggaa caatacggaa ctctcctact gcccccaaat tactaccatc
    22381 tactaactca tcaaaggcag taaaataagg cattcctgaa gcaatgcaga aaccttgatt
    22441 aacagcagat tctaaacttt cttttttaga agaatagcct ctaataatac catcattatc
    22501 aatataaagt aatcttgtta tttgtatatc ttcttcttca acaattttta aaatatcttc
    22561 tttttccaat ttaatcatcc tcctattaat ttaataatat atccttggta acattggatt
    22621 aatatggcac agatactctt ccatatctag atttatcttt ttagcaatat gagcaggatc
    22681 tataaatgaa tttttttgtt taccaattat aacaacttca tctccttttt ttattttacc
    22741 atcaacttct tttaaatcca ccattatatg gttcatagaa acagcaacta tatcatatct
    22801 ttgaccatca attaagatat agcctctacc tgctaatttt atagttaatc catctgcagt
    22861 gccaacaggt attgttgcaa tcaaagtttt tttctgacat ctatatccat catcatagcc
    22921 tacatattcg ccttgcttta ccaatttcac ttggcaaact tttgatttcc aggcaagagg
    22981 aaattttaaa tcaataattt ttctatactc tttgctcgga tataggccag ccaaacctat
    23041 tccgactcta accatatcca aatgagtctc gggcattttt agagttgaag gagttgatgc
    23101 tgcatgtaat attggaatat caataccaat attttttgct tcttctatta gccccaagaa
    23161 taaatttaac tgctttcttg ttttctcttt tttaaaatga tgggtagtag aaaattgagt
    23221 aaaagctcct tctatttcca tatgaggtga attttttata gtctcagcaa gttttgtaaa
    23281 attatcaggt gaaaccccta tcctatttaa tcccgtatta attttaatat ggaccttagc
    23341 tgataaattt aagttttttg ccttgttttt aattctttta actgtcctct tatcgtatac
    23401 agtcaatcga atctgttttt ttaaagcaat attaatatat gaataaggga tatcacataa
    23461 gattaaaatt ggtaatttga ttccttcttt tagaatagtc aaagcctctt ttatattcgt
    23521 taatcctaac caatcaacac cagccttttg aagggtttga gaatataaaa ccgtcccata
    23581 tccgttagca tttcctttta ctacccccat tagatactga tcttttaaaa aatcaccttt
    23641 gatcttatct acattttcat atattgcttc tttgtttatt tccaaccagg ttctataacc
    23701 ttccataagt cgccaacacc tttttatatg ttataaacct ttttagcatt tttccagaat
    23761 accatttcta atatttcctg tccatatttt tcattaaagt aatcctcttc aataagttct
    23821 ttaacaatat ttaacatttc cttttttgcc atccttgctg ccagccaatg aatgtcaggt
    23881 aaaacaaatc catctgaacc aaacatcagt ttattaaacg gacacatgtc tatcatatcg
    23941 cgtaatccct tcttcatccc taaaggaaca aaaggaacca tttcggacat atctagataa
    24001 atattaggat acatagcagc aagataacct gcttcaaaac tataaggata accaccatgt
    24061 acaagtacaa tatccgtctt gctatatttg ggatcatcaa taatactttt tagttttaat
    24121 ggattatttt ttaagagatc tatattagac tctccaaaag aagcatgaac ctgcattgga
    24181 atatttaatt gtccagcttt ctctaaagct gaaataaaaa agaaatctct gaattctttt
    24241 tcagtataat tttctttttt catcaatgac ctctcttttt tctcaatatt aagacctgta
    24301 cgatagccaa taatgctttt aatagctacg acatcaagtg ttgatttagc tttctctatt
    24361 tcatcataaa atctttcttc tgctttttta aaggaaacct tatcttccca taattcatca
    24421 acaatacttt caattctata catatattta acatcacaag gtaaaatgtt ttcaaaatct
    24481 tccaaactaa cacttgcagg cttataaccc aaatcaatta taaaagtatg catatttaca
    24541 tcttggcata atcttttcac atatccttta taatcagttt ccaataatcc tttacgttta
    24601 gttaatacat catcataacc tccttcaaaa tcaagcagat cttttaattc agatatatat
    24661 ttgtgataaa taagagagtt tttaagctgt ttttctggta tctcattaag cgataaactc
    24721 aatatattaa tcatttcacg atttttatca ctgacatcaa atagatgact atgattatca
    24781 attacaggaa tatcttttac attcattaac atcaacctcc attgatttac catcctgcat
    24841 aacgtgctaa atcatcttca gtaattttac cttcttttac ttcttttata gctagatcta
    24901 acttatctat tcctttgctt atctcatctt tagtaataac aaggggtgga gtgaattcta
    24961 atacattaga gtgaatacct acataaggtg caattagtcc attttggaaa gccctgaaac
    25021 taattttagc agtttctcta ctagcaggtt cttttgtctt cctatctttt actagttcga
    25081 ctccttgaac aagtcctcta cctcttatat ctcctattat ttcatattta ttttttagat
    25141 cttgtagctt atcgtgtaaa aattctccta cttcttgtgc tttgttcatt aatttttctt
    25201 tttcaacaac attgattgtt gctagtcccg ctgcagcact tactggatta cctgctgtcg
    25261 taaatagatg tgtagccaca cccgcatcta aaatttcact tcttcctata actccactta
    25321 acggcatacc agaagcaatc ggttttccaa aaactactgc atcaggaact acatcagatt
    25381 tttcaaatcc aaaaaactta cctgtccttc ctactccaat ttttacttca tcaaatatta
    25441 agtaaatacc atgcttatta caaatttttc ttaattcttg taaatatttg tcaggggcag
    25501 gcacaatacc cccatcacat tgaagagctt caagtaagat agcacttgta tcttcaggcg
    25561 gagatacaga agtaaatact tgattttcta aaaattttaa acattcaaga ttgcattcac
    25621 tttctttttt atcaaatggg caacgataac aatatggata tggtattttt gttacatttc
    25681 cactgccaat aaactttgcc tgagctgtgt gtcctgaaag ggataatgag cccattgttt
    25741 gtccatgata tgccccaaaa tatgatatta accgagctct atcattggct attggagcca
    25801 gttttgaaat acagtcatta gcatcagacc ctgataaccc aaaccatact ttctttttga
    25861 aatctccggg cacttttttt attaaatctt cagctaattt taccgacggc tcggtaatag
    25921 ttgtggtaaa agaagcaaaa ctattttttt caatttgctc ttcaactgat ttaactattt
    25981 ctggatgtcc atatcctgtg ttagctacag cccaattagc agaaaagtct aaataatcat
    26041 ttccatccac atctgttatt gtactacctt tagattcatc tgctacaagg gggaataatc
    26101 ttatcttcat tgaagtacct attacctttt catctctatc aattaaatct tttacactca
    26161 tttttactta atcctccttc ttactcttta atgagttaag atttattaca cttttttata
    26221 atctattttc taataaatat aaggttaaat tataaaacag taggaaataa gcgggaagat
    26281 ttagaactct tcccgcgatt tttaatttta tgctgtgata tctgcttttt tagcttcttc
    26341 tggtctggtc ttaaaccaaa tataaagtgg aataatccaa acaagtgata atggtataac
    26401 taaaggagaa cctttaaatg cataatacat ggtgaataag gtaccgccta tagctatagt
    26461 aaatgtgagt tttggtaatg gcatattaaa tgataaattt gaaacttctt ctggaaattc
    26521 ttttctgtat tttatagcag aaaagattga taataacata gatataaaaa cagcaacagt
    26581 aaattgcatt gtcaatgtta catatacaaa atctgtgttg aattttgcaa ttataataaa
    26641 tgcaatatta gctgcccaaa taaatactat gccccatatt ggtgtcctta attttggatg
    26701 tagcttccca aaaaatttgg gcattctttt ttttctagct tcactgaaaa ggatacggga
    26761 agcagaataa aatgtcccca tcaatagaca agttgaagct gccagtatag ctgcgaaatt
    26821 aataattcct cccccaacac taccaaatat tctagctgca gcagttacaa aaggagattc
    26881 agccattgat aattcttgcc aaggcactgt tcccaaggca ataaactgag cgccgatata
    26941 tagtacaata aatgttacac tcgcccaaac taatgcacga ggtaaatctc taacatcttc
    27001 acattctgca ccggcagtag ttaatgttgc agttcccata aaggcatatg cacccattgg
    27061 tattactaaa aacattgact ctaatccttc aggtaaaaat ggagtgaaat tttcaggtat
    27121 tccttttatt gcttctggaa atccaaaaac aactaatcca gccatgaccc caaccagtcc
    27181 aaatgtcatc cacatttggg ctcgagcagc tattaaacta ccaagtatgt ttaatatagc
    27241 aaatattgat actgctatag cagccagcac aagttcatta attggaatat aataagataa
    27301 aaatctaccg agtgctaaag aaatagcacc accaacaata atccaagcaa atgcccagga
    27361 aaaacctgca agaaaacccc accagggacc aaaaaacctc ttaacccagg gccccatagc
    27421 acctgcagaa ggcatcccta cagctagttc aacaagtgca agcattaaga ataactgtaa
    27481 aactccagct ataaaaaatg acacgataga agctggtccg gcagtttgta taccttcacc
    27541 taataagaga aaaatcccat cacctacaac agaacctact ccaagagccc aaatatgcca
    27601 aaactttagg tttctaagag gttttacttc aagaagattt ttttcactac tcaattaaac
    27661 tcctcctttg attaatgtat tattgcaaaa ttattataaa acacattaga cataaaacta
    27721 tgctaatcta ctaacttctc aactttcact ttctgattaa ataccaaaaa tatttttaat
    27781 ctgaaatatt atttctaaat cacctcctta tggtaatttt aatcttctcg gctaaatcat
    27841 cactctatat ataagggctc acggccctta cttacttttt tcacctccac aagaactgct
    27901 aatatcccta ttaatttagt actttagacc tattgacaaa ctcgatttct gatttttggc
    27961 gcagccacca taattgaaat ataggggatt ttttacccta aatctcaatt aaagggtgat
    28021 cactgtgttc aaaaaccaaa tgtctaatca acaatttaaa tcttttatca aaaagaatct
    28081 tattgaatat tacgacaaat ctaacttcta tgattatatc acttcaaagg ttaatattat
    28141 ccacaaaatc tacattactg atctcaccgc tgtaagaact atacttttag acacctttca
    28201 acctagaggt caaaaaccat gggaccccat ctgccttttt agaacttatt ggctgatttg
    28261 ccaatatggc agcggctcta tctctcaatg gatagaaaca cttaaaaacc ctttctgggc
    28321 tatcctttca ggctttactc ccggcgatat tcccggaata ggtaccttat atgactttga
    28381 aaaaagaata ctcgattttg atatgggcca gagaagcaaa cgactgcaaa aaaagtataa
    28441 ttttaaccgt aaacctactc aaaaacttaa gaaaaatcaa aaacaaaaac ctaaaaaagc
    28501 aggtatagtt aaaaacatg
//
LOCUS       DATGCJ010000128        15646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_60719, whole genome shotgun sequence.
ACCESSION   DATGCJ010000128 DATGCJ010000000
VERSION     DATGCJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60719"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VKN64_06410"
     CDS             complement(<1..1528)
                     /locus_tag="VKN64_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HMA59907.1"
                     /translation="MNRYIKTIVVLLIFLALFSVPISAERPPIMMVDEIKSGQSGYAK
                     TVFEGTKVEEFPIEVINIVEGQSIDTDLILIKASGEKIEKSGGIASGMSGSPVYINDK
                     LVGAISYKWQNADHEYALVTPILEMMDINKEEEQTSVIEGIQAASTPIMIGGLSGRAL
                     KRLKTGLDIADSRFVSSSSSTSKGTEGFKLEAGSALAVQLVRGDVSVASIGTVTLVDQ
                     GEVYAFGHPFMNKGKSNYLMSGAYIDAIIPSRTQPFKVGSPLDELIGVIEDDRNAGIF
                     GRLNKFPNIIPLTVKVEDKNLNQTKEVRTQIINDEKMLDELGSSIILQSIDSTLDRIG
                     YGTSNIKITIMGSNLPDGKIVKENMYYSNNDIAVTTLNDYFRMLDMITRNPFKEINIF
                     DMKVDVEISKDHDIALIQEAELLNKEVYPGSELDVKVVLRPYRADSISRELSIKIPED
                     INTGMATLAISGGYGGLLNRRDPQEDNGNDLNHTVIEGYKSFDEMIKDYLEAPMNNEL
                     T"
     gene            complement(1556..2575)
                     /locus_tag="VKN64_06415"
     CDS             complement(1556..2575)
                     /locus_tag="VKN64_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMA59908.1"
                     /translation="MGLSKKIGIDLGTANVLIYEKGKGVVVQEPSVVAIDKNNDKVLA
                     VGQEARRMLGRTPGNIVAIRPLKDGVIADFEVTELMLKHFISKVVKRKFFFKPTIMIC
                     IPEGITGVEKRAVMEAASQVGSRDTYLIEEPLAAAIGAGLPVEDPSGSMIIDIGGGTT
                     EIAVISMGGIVVSESLRIGGDKFDEALVRYIRDKYNMVIGEKTAEEIKMEIGSAWIDG
                     ETKDYEVRGRDLMSGMPKNLTISSPETVEAFKENITTIVESVRHVLEQTPPELSSDIM
                     DKGIILTGGGSLLHGMDTLLSKETKIPVSPAASPLSCVAEGTGIALEEIDRLKEILTS
                     SEMLK"
     gene            complement(2649..3932)
                     /locus_tag="VKN64_06420"
     CDS             complement(2649..3932)
                     /locus_tag="VKN64_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMA59909.1"
                     /translation="MGSEAVSELARIEEWMVQNNVADKEIFVLGILIFLSLLVIFVAK
                     KYQIPIVVGYVFLGILLSTDVIEWMPFLSQAQKEWYNYSLESISYVTNIALAFIAFTI
                     GSELSVKLLKKMGKSIVYIVLLEATAAFTLVFLAVIALGKPIYIGMLLGGIASATAPA
                     ATVMVLKEYNAEGPLTSMIMAIVGIDDAIALILFSIVEPIAYARHYGQETLSFLSSIS
                     QPLMEITFSVLLGIALGYVGQKIIVNVEDKTKKIMTLVTNIIVASAIAIYFDFSPLIT
                     NMVVGFTYRNFARKNLGIADYLETMTTPVYAIFFILAGTEIQLSNVASVSFLALAFTY
                     LIARMAGKVGGASLGAIIGGAPEKIKKYVGLGLFPQSGVAIALAYMVQQDFASDPQVG
                     TLIFNTLLFTAALTEVIGPFATKYAVFKAGEAKAS"
     gene            complement(4022..5269)
                     /locus_tag="VKN64_06425"
     CDS             complement(4022..5269)
                     /locus_tag="VKN64_06425"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMA59910.1"
                     /translation="MAKFVINGGNTLEGEVKINGAKNAALPILAATLLADSPSILKDI
                     PDLRDVSNLCEIIKGMGARVERSGHTVEIDPNGLDTYQTDKELAKKLRASYYILGVML
                     AKHGEARTSLPGGCNIGNRPIDLHLKGFKALGADVSLNHGIVEVKADKLVGDKIYLDY
                     PSVGATINIMLAASLAEGKTVIENAAREPELVDLANFLTVMGAKIKGVGTDIIKIEGV
                     DSLEGKEHRIIPDRIEAGTYMIAAALTKGDVFVKNVLTEHIKSLIAKLHEMGLEMVED
                     ISGVRVKGNGRLDSIDVKTLPYPGFPTDLQSQMMVLLTQTDGTSIIKETVWENRFMHV
                     DELKRMGADITIDGHSALIRPSRLSGAEVTVTDLRAGAALILAGLIGDGQSEVRKIHH
                     VERGYEGIEKKLSSLGADIERVK"
     gene            complement(5842..6267)
                     /locus_tag="VKN64_06430"
     CDS             complement(5842..6267)
                     /locus_tag="VKN64_06430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HMA59911.1"
                     /translation="MAPKNKILLEIITPKKTVYKEEVDGVEAPAVDGLIGILPRHAPL
                     ITALKTGVIRVKIDNKERFISVSEGIMDVKPDQVNIVARTAELPEEIDLERAKQAKER
                     AEERLEETSSKIDHYRAEGALERALARIKAAEKHHSEYY"
     gene            complement(6270..7727)
                     /gene="atpD"
                     /locus_tag="VKN64_06435"
     CDS             complement(6270..7727)
                     /gene="atpD"
                     /locus_tag="VKN64_06435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMA59912.1"
                     /translation="MSDDQNLGKVVQVIGPVVDVEFPSGNLPEIYNAVEVIRDEEEDE
                     TLVIEVMQQLGDNRVRCVAMDSTDGLVRGMQAVDTEGPISVPVGEEVLGRMFNVVGDT
                     IDNEGEVGTDERWPIHREAPGYEEQQTSTELFETGIKVIDLLAPYTRGGKVGLFGGAG
                     VGKTVLIQELINNIALEHGGYSVFSGVGERTREGNDQWLEFQEADILDKVALVFGQMN
                     EPPGARMRVGLTGLTLAEYFRDELGQDVLLFIDNIFRFIQAGSEVSALLGRMPSAVGY
                     QPTLAYDVGALQERITSTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQI
                     VEKGIYPAVDPLDSTSNIMDPRIIGEEHYGVARDVQEILQEYQDLQDIIAILGMDELS
                     EEDKIVVNRARRLERFLSQPFFVAEQFTGQPGTYVELDDTIRGFKEIVNGKHDDLPEE
                     AFYMVGTIDEAVDKAEKLRAEGKARKEKSEKKQGE"
     gene            complement(7741..8622)
                     /gene="atpG"
                     /locus_tag="VKN64_06440"
     CDS             complement(7741..8622)
                     /gene="atpG"
                     /locus_tag="VKN64_06440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMA59913.1"
                     /translation="METMRDIKRRINSVKNTKKITRAMKMVAAAKLQNSQKYARQAKP
                     FFTKTRKILSDVYKFSGIKDEHPLLKKSEGDKKLLIMISGDRGLCGAYNAKVIEEAED
                     FLNKNSDTEIYALGSKARDYFKKRNYDFIKEYEQLEDYPSFYFARQLTDEIIEIFKGK
                     KFNQINVIYTHFESALTQHVKNITLLPIDAEEIDKELSDKGEKASEEHVDYIYEPSAK
                     EILDLLLPKYVNNTMYSILLESKASEFGSRMTAMDNATENANEMIDDLTLEYNRARQA
                     AITTEITEIVGGAEALK"
     gene            complement(8657..10270)
                     /gene="atpA"
                     /locus_tag="VKN64_06445"
     CDS             complement(8657..10270)
                     /gene="atpA"
                     /locus_tag="VKN64_06445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMA59914.1"
                     /translation="MELRPEEISSIIKEEIKNYDKKIESVGVGTVLDVGDGIAHVYGL
                     EDAKSNELLEFPNGVYGMALNLEEDNIGVVLLGNETKISEGDTVKRTGNVVEVPVGEE
                     LLGRIVNPLGMPLDGKGEINTEKTRPVEKKAPGVIERQPVEEPLQTGLKAIDSMVPIG
                     RGQRELIVGDRQTGKTAIAIDTIINQKDQDVICIYVAIGQKESSIARLTEELQERGAM
                     DYTIVVSASASKPAPIRYLAPYAGCAMGEHFMYEQDKDVLVVYDDLSKHAVAYRAMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDERGAGSLTALPIIETQAGDISAYIPTN
                     VISITDGQIYLESELFFSGVRPAINVGTSVSRVGGNAQIQAMKDIAGTLRLDLSQYRE
                     LEAFAQFGSDLDEATQQKLARGERIVETLKQPQYTPMPVEKQIIIIYTVVKGHLDDIP
                     VSNIERFEEEFLTYIDNNHEYLQQKLKKEEELTEEIENKLVSVIEEFKEIFEVKEESI
                     VSTEDELMQESEETEETAEIEEEEVEEEA"
     gene            complement(10277..10837)
                     /gene="atpH"
                     /locus_tag="VKN64_06450"
     CDS             complement(10277..10837)
                     /gene="atpH"
                     /locus_tag="VKN64_06450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMA59915.1"
                     /translation="MKKNEIARQYSKALYEIGDEKDNLFELYDELKELNKLIKENENL
                     SEALFHQRVLVEEKQRVFKRVFEDKLSDDIYKFMMLLIEKRRIYFLENIVAEFRKQVY
                     KHENIITVEVTTAVEMSSETKDKLKAKLNEHVDKKIEMEEHCDPDIIGGMVLKIGDYL
                     IDGSIKSKLESLEEQIKKIPLQEIGV"
     gene            complement(10834..11334)
                     /gene="atpF"
                     /locus_tag="VKN64_06455"
     CDS             complement(10834..11334)
                     /gene="atpF"
                     /locus_tag="VKN64_06455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMA59916.1"
                     /translation="MVEINTTLLWQVFNFFVLMWLLKRYLYGPVKEILDKREKTIKNE
                     IEEAEEKHAEAMELKEEYKSELRRAREKSSDIIEKAERRARERAKEIINNAKEQAEKI
                     EKDKMREIQQAKVEARKEIRDEFADVTLQAAAQMIGRELDREKHEELMAQYIETLDQE
                     KLGDVQ"
     gene            complement(11443..11700)
                     /gene="atpE"
                     /locus_tag="VKN64_06460"
     CDS             complement(11443..11700)
                     /gene="atpE"
                     /locus_tag="VKN64_06460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMA59917.1"
                     /translation="MVEFSPQVIDTIIRAAALLGAGFAMIAGIGPGIGQGYAAGKAVE
                     SVARDPEARGNIITTMLLGQAVAESTGIYSLVIAIVLIFTI"
     gene            complement(11702..12145)
                     /locus_tag="VKN64_06465"
     CDS             complement(11702..12145)
                     /locus_tag="VKN64_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HMA59918.1"
                     /translation="MKYINDPRQMQIEIIKRTYLISSIPILYSLISGNFETLLGFIFG
                     LVISTLMLRLKAINIQKALDMQEGKAESFIRNRYFIEYGIYFVVLIVARKNPSLNFLA
                     AAIGLFMMKFTVIMWAILDMARGGLNKKLEEYKDLKDDKFEKGGK"
     gene            complement(12168..12422)
                     /gene="atpE"
                     /locus_tag="VKN64_06470"
     CDS             complement(12168..12422)
                     /gene="atpE"
                     /locus_tag="VKN64_06470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMA59919.1"
                     /translation="MIEFSPETIEMIIRSVSVIAAGLALIAGVGPGVGMGFAAGKATS
                     AVRRQPEARGAIVTTMLLGQIISGSTGIYSLVIAIYLVFT"
     gene            complement(12461..13120)
                     /gene="atpB"
                     /locus_tag="VKN64_06475"
     CDS             complement(12461..13120)
                     /gene="atpB"
                     /locus_tag="VKN64_06475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMA59920.1"
                     /translation="MNPGPKVVTHLFGKSYLPITDTLIVGWVSVVLIIILAKYLTSEL
                     KIRPGKKQNIAEFLITAIHDQIEPMLPGEGWRFLPFIATIFIYVGVGNLIGLLPGVAS
                     PTGDLNTTLGLALVVFLTSHIEGMREKGVWGYIKSFGQPVIFLLPLNVVGELAKPISH
                     SFRLFGNIVGGGIIITLIYQAAPGLIPVPIHAWFDFFIGLIQALIFGMVAIAYISVAK
                     S"
     gene            complement(13145..13387)
                     /locus_tag="VKN64_06480"
     CDS             complement(13145..13387)
                     /locus_tag="VKN64_06480"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HMA59921.1"
                     /translation="MKPQDWRKIMRTMGLLTQLGIVIIVNIGVGFYLGLKLDQWLGYT
                     YLFKILGIIIGIGSGFYSDYKLILSSMEDDEDDEQD"
     gene            complement(13626..14675)
                     /locus_tag="VKN64_06485"
     CDS             complement(13626..14675)
                     /locus_tag="VKN64_06485"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HMA59922.1"
                     /translation="MISKFILIFVSSLIISYLLTPLVKKMANVINGFDHPSKRRINTK
                     PIPNTGGIAIFTAFIISYFLFNDHSLSTYGIMFGATFIFILGLIDDIFEISPLVKLLG
                     QIGAASILIYYGVQINFITNPLGGMIYLGYFSFPVTILWVVGITNTVNLIDGLDGLAA
                     GISIIATMTLFAVAMQEYRLIPAVLAIALAGSLSGFLKFNFHPAEIFMGDSGSMFTGF
                     ILAAISATGALKSAAAVTIVVPILALGVPIFDTIFAIIRRVYHNKPIGEADRGHIHHR
                     LIALGWSHRQAVIIVYGSSLMLGATALIINGLNIKNGMILFGLAITGVIYGAWKLGIF
                     STELSPDSQALEESN"
     gene            complement(14701..15330)
                     /gene="upp"
                     /locus_tag="VKN64_06490"
     CDS             complement(14701..15330)
                     /gene="upp"
                     /locus_tag="VKN64_06490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HMA59923.1"
                     /translation="MSETILIEHPLIQHKLTLIRDKTTGPKEFRELVDEVSTLMVYEV
                     TRDLPLEDVEVETPICKGTFKMIEGKKLGIVPILRAGLGMLNGVLQLIPTAKVGHVGL
                     YRDPDTLEPVEYYCKLPTDVTERDLLVVDPMLATGGSACAAIDFVKDRDPRNIKLVVL
                     IAAPEGIERVQKEHPDVDIYTAAIDEKLNDHAYIVPGLGDAGDRLYGTK"
BASE COUNT         4581 a         3442 c         2447 g         5176 t
ORIGIN      
        1 atgttaactc attattcatg ggtgcttcaa gataatcttt gatcatttca tcaaagcttt
       61 tatatccttc gattacggta tggtttaaat cattaccatt atcttcctgc ggatctcgcc
      121 tgtttagaag acctccatag cctccactaa tggccagggt tgccataccg gtattaatat
      181 cttccggtat cttaattgaa agctctcgac taatagaatc cgctcgatat ggtcttaata
      241 ctactttaac atctaattca ctacccggat atacttcttt atttagtaat tctgcttcct
      301 gtattaaggc tatatcatgg tctttgctaa tttccacatc aaccttcata tcaaatatat
      361 ttatttcttt aaatggattc ctggtaatca tatcaagcat tctaaagtaa tcgtttaaag
      421 tagtaacagc aatatcatta ttactgtaat acatgttttc cttgacgatc ttaccatctg
      481 gtaaattgga acccatgata gttatcttaa tattagaagt accataacca attctatcta
      541 aggtactatc aatcgactgt aaaattatac tgctgcccag ttcatccagc atcttctcat
      601 cattgatgat ctgggtccta acttctttag tttgatttaa atttttgtct tccactttaa
      661 cagttaaggg tattatattg ggaaatttgt ttaatctccc gaaaataccg gcattccgat
      721 cgtcttctat aacaccaata agttcatcta gtgggcttcc caccttaaag ggctgagtcc
      781 gactgggaat aatagcatca atataggctc cactcattaa ataattgctc ttacctttat
      841 tcataaaagg gtgaccaaat gcataaactt ctccctggtc aactaaggtg accgtaccaa
      901 ttgaagcaac actaacatca cctctaacta gctgtacagc cagggcacta ccggcttcta
      961 atttaaagcc ttccgtccct ttgctggtac ttgaagaact gcttacaaaa cggctgtccg
     1021 cgatatctag tccagtcttt aacctcttta aagctcgtcc gcttaagccc ccaatcatta
     1081 ttggggtact agctgcttgg atgccttcta taactgaggt ttgctcttct tctttgttaa
     1141 tatccatcat ttccaatatg ggtgtaacaa gtgcatattc atggtcagca ttctgccatt
     1201 tataactaat agcccccact aatttatcat taatatagac gggactacca ctcataccgg
     1261 aagcaattcc acctgacttt tcaatctttt caccagaggc tttaatcaat attagatcag
     1321 tatcaatact ttggccttca actatattta taacttcaat agggaactct tctactttag
     1381 taccttcaaa tacagttttt gcatagccgg actgaccgga tttaatttca tccaccatca
     1441 taatcggtgg tctttctgcc gaaataggga cactaaataa tgctaagaaa attaataaaa
     1501 caactatggt tttaatatat ctattcaaat tatcaaatcc cttccacttt ctttactact
     1561 tcagcatctc actcgaggtt aatatttctt ttagtctatc tatttcttct aatgctatgc
     1621 cggtaccctc tgctacacaa gaaagaggtg aagctgctgg tgataccggt atcttggttt
     1681 cttttgatag aagtgtgtcc atgccgtgta agagtgaacc tcccccggtc aaaataatgc
     1741 ccttatccat aatatcagaa gaaagctcgg gcggggtctg ttctaaaaca tgtcttacac
     1801 tttccacgat cgtagtgata ttctctttga atgcttcgac tgtttcgggt gaagaaatag
     1861 tgagattctt aggcatacct gacattaaat ctctaccgcg cacttcataa tctttggttt
     1921 ctccatctat ccaggctgaa cctatttcca ttttaatttc ttcagctgtt ttttccccaa
     1981 ttaccatatt atacttgtct ctaatatatc taactaatgc ttcatcaaat ttatctccac
     2041 cgattctaag cgattcgctt accacgatac cgcccataga aatgacagct atctcggtgg
     2101 tgccgccccc gatatctata atcatactac cgctcgggtc ttcaaccggg agtccagctc
     2161 ctattgcggc agccagtggt tcttctatta gatatgtatc acgggaacct acctgagagg
     2221 cggcttccat gacagctctt ttttctacac ctgtaatacc ttccggtata cagatcataa
     2281 tggttggttt aaagaaaaat ttcctcttta caacctttga aataaagtgt tttaacataa
     2341 gctctgtaac ttcaaaatca gcaattactc catcttttaa aggcctgatt gccacgatat
     2401 taccgggagt tcggcctaac atacgtctgg cttcttgtcc aacagccaaa actttatcat
     2461 tatttttatc tattgctaca acagatggtt cctgtacaac tactccttta cctttttcat
     2521 atattagaac attggcagtt cctagatcaa tacctatttt ttttgataaa cccatttatt
     2581 aatttacctc cttgcgaaac tgttaaaatt tattaaaaat atttttttca ccatttgatt
     2641 aaacttgctt aacttgcttt tgcttcaccg gctttaaata cagcatactt ggttgcaaat
     2701 ggtccgataa cttcagttaa agctgctgtg aataagaggg tgttaaaaat taatgttccc
     2761 acctggggat cagaggcaaa atcctgctga accatatagg ctaaagcaat tgccaccccg
     2821 ctttggggga acaatcctaa ccctacatac tttttaattt tttcgggggc acctccgata
     2881 attgcgccta aggatgcgcc gccgaccttg ccagccatac gtgctattaa atatgtaaat
     2941 gctaaagcta agaaactgac agaagcaaca ttacttaact ggatctctgt accggctaaa
     3001 atgaaaaata tggcgtagac tggtgtggtc attgtctcta aataatcagc gattcctaaa
     3061 ttcttacggg caaagttacg gtaagtaaaa ccgaccacca tattagttat caaaggtgag
     3121 aaatcaaaat atatcgcgat tgcggaagct actattatat tggtaacaag ggtcatgatc
     3181 tttttggtct tatcttcaac attaactata attttttgac ctacataacc caatgcaatt
     3241 cccaataata cagaaaatgt tatctccatc agcggttgtg aaatgctcga caaaaacgag
     3301 agtgtctctt gtccataatg ccttgcatag gcgattggtt cgacaatact aaataaaatt
     3361 aaggcaatag catcatcgat accgacaata gccattatca tggaagttaa aggaccttct
     3421 gcgttatact cctttaaaac catcaccgtg gctgctggtg ccgtagcaga ggcaataccg
     3481 ccgagtaaca ttcctatata tataggttta cctaaagcaa ttaccgctaa aaatacaagt
     3541 gtaaaggcag ctgtagcttc aagtaaaact atatatacaa tacttttccc catctttttt
     3601 aatagtttaa ccgataactc actcccgatg gtaaaggcga taaaggcaag tgctatattt
     3661 gttacataac ttatactctc taaagaataa ttataccatt ccttttgggc ctgagataaa
     3721 aaaggcatcc attcaatgac atcggtgctt agtaaaatac ctaaaaatac ataacccact
     3781 actatgggaa tctgatactt cttggcaaca aagatgacta aaagtgaaag aaaaattaaa
     3841 atacccagga caaatatctc tttatctgca acattgtttt gaaccatcca ttcctcgatc
     3901 ctggctagtt ctgatactgc ttctgaaccc ataatacttt ccacctctat ttagttgtaa
     3961 gttttatata taaatattaa aaccctggaa tgaggttcca ggatttcaaa agaattaaac
     4021 actatttcac tctctcaata tccgcaccca aactggataa ctttttctca ataccctcat
     4081 atcctctttc tacgtgatga atttttctaa cctcggactg accatcacca attagacctg
     4141 ccaaaatgag ggcagctccg gctcttaagt cagttacggt tacttctgct cctgataatc
     4201 tactcggcct gatgagtgcg ctgtggccgt cgatagtaat atcagcaccc attcgcttta
     4261 attcatcaac atgcataaat ctattttccc agaccgtttc tttgatgata ctggttccat
     4321 cagtctgagt taaaagtacc atcatctggg attgtaaatc ggtcggaaag cccggataag
     4381 gtagagtctt cacatcaata gaatctaatc tgccgttccc tttaactctt accccggaaa
     4441 tatcttcaac catttccaat cccatctcat gtaatttagc tatcaaagac ttaatatgtt
     4501 cagtcaagac attttttaca aagacatcac ctttagttaa agcggctgca atcatataag
     4561 tacccgcttc aattctatca ggtataatcc tgtgttcttt accttccagg ctgtcaacac
     4621 cttctatttt aatgatatca gtacctacac ctttaatttt agctcccatt acagttaaaa
     4681 agttagctaa atcgacaagc tctggttcac gggcagcatt ttctattact gtcttacctt
     4741 ctgcaagact tgctgccaac atgatattaa tggtcgcccc tacactgggg taatcaagat
     4801 agatcttatc cccaactaat ttatctgctt ttacctcaac aattccatgg tttaatgata
     4861 catctgcccc tagggcctta aaacccttga gatgtaaatc aatcggtcta ttaccaatat
     4921 tacaacctcc gggtagagaa gttcttgctt caccatgctt agccagcatc acacccagga
     4981 tataataaga agctcttagc tttttcgcta attccttatc agtctgatat gtatctaaac
     5041 cattaggatc tatttcaaca gtatggccgc tcctttcaac acgagcaccc atccctttga
     5101 tgatttcaca taagtttgaa acatctctca aatctggaat gtctttcaat atactgggag
     5161 aatctgctaa tagtgtagca gccagtatgg gtaaagcagc atttttggca ccattaatct
     5221 taacttcacc ttctaaggtg ttacccccat taataacaaa tttggccaat ttatatgctc
     5281 ctttcattac cgaaaaaatc tctatataat aagttctata ttcatctaaa aagaccttca
     5341 tttagagtct ttacattcct agtaagaata tcatattttt tgtatttttt caacaatact
     5401 tgacccttat ttaaaccaat ctgctattga aaattccaaa ttatgactgg aggtagatat
     5461 tttgtaaaaa accccgagta ttcggggtat attaatgcta attcactgat gtctttaact
     5521 atatcagata tattataatt tttaaaatca aagttaaatt atcctcaaat ttaattatta
     5581 ttgagcttag ttgaaaactg aatataataa gataattttt aataaaaata tattatggac
     5641 ttttataaat caaattccgt gccttctaat acttattatt attttactcc caaaatgtca
     5701 aataaatatg tgaagcagaa tattatattg ttttatctga acatgttgat tttaatatat
     5761 gattagctca atactaatca atcccttatt ctaataaaat tagaaaaagc cccaacaaaa
     5821 tggggcttta aattacagat attaataata ttcactatgg tgtttttcgg ctgccttgat
     5881 acgggctaag gctctttcta aggcaccttc tgcccgatag tggtcaattt ttgaactggt
     5941 ttcttccagt ctctcttcgg ctctttcttt cgcctgttta gctcgttcta agtcgatttc
     6001 ttccgggagc tcagccgttc tcgccactat gttaacctga tccggtttaa catccatgat
     6061 accttcacta accgagataa atctttcttt attatcaatt tttactctaa tcacacctgt
     6121 tttcaaggcg gtgattaaag gagcatgacg gggaagtatt cctatcaatc cgtcaacagc
     6181 cggagcttca acaccatcaa cttcttcttt gtatacagtt tttttaggcg ttatgatttc
     6241 cagcaaaatc ttatttttcg gtgccatgat tattcacctt gtttcttttc agacttttct
     6301 ttccttgctt taccttcagc tcttaatttt tctgccttat cgacagcttc gtcaatagta
     6361 ccaaccatgt agaatgcttc ttccggtaag tcgtcatgct taccattaac gatctcttta
     6421 aagcctctaa tggtatcatc tagttcaaca taagtaccgg gctgaccggt aaactgttcg
     6481 gccacaaaga aaggttgaga taagaatctc tcaagtcttc tcgctctatt tacaacgatt
     6541 ttatcttctt cagataattc gtccataccc aggatagcta taatgtcctg taagtcctgg
     6601 tattcctgta agatctcctg cacatcacgg gcaaccccat aatgttcttc cccaataatt
     6661 ctcggatcca tgatatttga agttgaatcc aggggatcca ccgcaggata aatacccttc
     6721 tcaacgatct gtctggacag tacagttgta gcatccaggt gagcaaaggt tgtggcaggt
     6781 gcagggtcag tcaagtcatc tgccggtacg taaatagcct ggacagatgt gatcgaaccc
     6841 tttttagttg atgtaattct ttcctgtagg gcacctacat cataggccag ggtcggctgg
     6901 taaccaaccg cagaaggcat tctacctaat aaagcagaaa cctctgaacc ggcctgaatg
     6961 aaacggaaaa tgttatcaat aaataaaagt acatcctgac ctaactcatc gcggaaatat
     7021 tcagctagag ttagtccagt aagtccaact ctcattctgg ctcccggtgg ttcattcatc
     7081 tgaccaaata ctaaagcaac tttgtccaag atatccgctt cttgaaattc aagccactgg
     7141 tcattaccct ctctggttct ttctcctaca ccagagaaga cagaataacc accgtgctct
     7201 aaagcaatat tatttattaa ttcctggata agtacggttt tacctacacc ggcacctcca
     7261 aagagtccga ccttacctcc ccgggtatag ggggctaaaa ggtcaataac tttgatcccg
     7321 gtttcaaata gttcggttga agtctgttgt tcttcatatc caggggcctc tctatgtatg
     7381 ggccatcttt cgtcagtacc aacttcacct tcattatcaa tagtatcacc gacaacatta
     7441 aacatacggc ctaatacttc ttcaccaacg ggaacactaa ttggtccctc ggtatcaaca
     7501 gcttgcattc ctcttaccag gccatcggtc gaatccatcg caacacagcg aaccctatta
     7561 tctccgagct gctgcattac ctctattacg agagtctcat cttcttcttc gtctcttata
     7621 acttctacag cattatatat ttcaggtagg ttaccacttg ggaattcaac atcgacaacc
     7681 ggtccgataa cctgaactac tttacctaaa ttttggtcgt cactcacaaa aactcctcct
     7741 ttatttcaat gcttcagccc cgccgactat ctcagtaatt tctgttgtaa tggcagcttg
     7801 acgggctcta ttatattcta acgttaaatc atcaatcatt tcattcgcat tttcagtagc
     7861 attatccatt gcagtcattc tagacccaaa ttcacttgct tttgattcta ataaaattga
     7921 atacatggta ttattaacat acttgggtaa aagcaaatct aaaatttctt tggctgatgg
     7981 ttcatagata taatctacat gttcttcact tgccttttca cctttatcac ttaattcctt
     8041 atcgatctct tctgcatcga taggtaataa agtgatgttt ttgacatgct gggttaaggc
     8101 agattcaaaa tgagtatata ttacatttat ctggttaaac tttttaccct taaagatctc
     8161 tataatttca tcggtcagct ggcgtgcgaa atagaaactg ggataatcct ccagctgttc
     8221 atattcctta atgaaatcat agtttctttt tttaaagtaa tctcttgcct tacttcccag
     8281 tgcataaatt tctgtatcag aatttttatt caagaaatct tctgcttcct cgattacctt
     8341 tgcattatat gcaccacaaa gtcctctatc tccactaatc ataatcagaa gtttcttatc
     8401 tccctctgat tttttgagaa gtggatgttc atccttaatt ccagagaatt tatagacatc
     8461 ggaaagtatt tttctggttt tagtgaagaa tggtttagcc tgacgggcat atttttgaga
     8521 gttctgcagt ttagctgcag ccaccatctt catagccctc gtaatttttt tagtattttt
     8581 aacactattt atacgacgct taatatctcg catcgtttcc ataaacattc acctcaatct
     8641 ttaaaaggtc ctatctttaa gcttcttctt caacctcttc ttcttcaatt tctgctgttt
     8701 cttcagtttc ttccgattcc tgcattaatt catcttcagt tgaaacaata ctttcctctt
     8761 taacttcaaa gatctcttta aattcttcaa ttacagatac cagtttattc tcgatttctt
     8821 cggttaattc ttcttctttt ttcagttttt gctgtaaata ttcatggttg ttatcaatat
     8881 aggtcagaaa ttcttcctca aatctctcaa tattgctgac cgggatatca tctaaatgac
     8941 cttttacaac cgtatagatt attataatct gtttttcaac cggcattggt gtatactggg
     9001 gctgttttaa ggtttcaact attctttccc ctctggccag tttctgctgg gtagcttcat
     9061 ccagatctga accgaactgg gcaaaagcct ctagctctct atactgagac aggtccaatc
     9121 ttaaggtacc agcaatatct ttcatggcct gaatctgtgc attacctcca acccttgata
     9181 cagaagtacc aacattgatc gcaggtctta cacctgagaa gaataattca ctctctagat
     9241 agatctgacc atctgtaatc gagataacgt tggttggaat ataggcagat atatcaccgg
     9301 cctgagtttc tataataggt aaggctgtta aggaaccagc ccctctttca tcactaagtt
     9361 tagcggctct ttcaagtaaa cgagaatgta agtagaaaac atcaccagga taagcttccc
     9421 gtccaggtgg tcttcttaaa agcagtgaca ttgcacgata ggcaaccgca tgctttgata
     9481 aatcgtcata aacaactaaa acatctttat cctgctcata catgaaatgt tcacccattg
     9541 cacaaccagc atagggtgca aggtatctaa taggagcagg tttagaagca gaagcactta
     9601 ctactatcgt ataatccatg gctccgcgtt cctgcagttc ttcagttaat ctcgcaattg
     9661 atgattcctt ctgtccaatt gccacataga tacagataac atcctgatct ttctgattga
     9721 taatggtatc gatagcaatc gcagttttac cggtctgacg gtcaccaaca atcaactcac
     9781 gctggcccct tccgattggt accatggagt caatcgcttt taaaccagtt tgcagtggtt
     9841 cttctaccgg ctgtctttca ataacaccgg gcgctttttt ctctaccggt cttgtttttt
     9901 cggtattaat ttcgccttta ccgtccagcg gcataccaag cggatttaca atacgtccta
     9961 aaagttcttc acccacaggc acctctacta cattacctgt tctcttgact gtatcgcctt
    10021 cactaatttt tgtttcatta cctaataata ctacacctat gttatcttct tctaagttta
    10081 atgccatacc ataaacgccg ttgggaaact ctagtagttc attggactta gcatcttcta
    10141 gtccataaac gtgggcaatt ccatctccaa catctaatac agttcccacg ccgacagatt
    10201 caattttctt atcataattt ttaatttctt cttttataat cgaactaatc tcttccggtc
    10261 taagttccat atcatattac accccaatct cctgtaatgg aattttcttg atttgttctt
    10321 caagggattc taacttgctt tttatactac cgtcaattag gtagtctcct atttttaaaa
    10381 ccatgccgcc gattatatcg ggatcacagt gttcttccat ttcgatcttt ttatctacat
    10441 gttcattaag ttttgctttt aatttatctt tggtttcaga actcatttca actgcggtag
    10501 taacttccac agttatgatg ttctcatgtt tatagacctg ttttctgaac tctgccacta
    10561 tgttttctaa aaaatatatt ctgcgttttt cgattaaaag catcataaac ttatagatgt
    10621 catcagaaag tttatcttca aaaactctct taaagactct ctgcttttcc tctactaaga
    10681 cacgttgatg aaaaagtgct tcacttaaat tttcattctc ttttattaat ttatttagtt
    10741 cttttagttc atcataaagt tcaaataggt tatctttttc atcaccaatt tcataaaggg
    10801 ccttactata ttggcgggct atttcgttct tcttcactga acatcaccca atttctcttg
    10861 atcaagtgtc tcgatgtatt gagccatcag ttcttcatgt ttttcacggt ccagctctct
    10921 gccaatcatt tgagcagccg cctgtaaagt gacatcagca aactcatctc tgatctcttt
    10981 tctggcttct actttggcct gttggatctc tctcatctta tctttttcga tcttttcggc
    11041 ctgttctttg gcattattta taatttcctt ggcacgttcc cgtgctcttc tttccgcttt
    11101 ttcaataata tctgaagatt tttctcgggc ccttcttagt tcagatttat attcttcttt
    11161 taattccatt gcttctgcat gtttctcttc tgcttcttct atttcgtttt taatcgtctt
    11221 ttctctttta tctaaaattt ctttgacagg accatatagg tatctcttca aaagccacat
    11281 aagtacaaaa aagttaaata cctgccataa taaggttgtg tttatttcaa ccaaagtcct
    11341 tcacctcccg tgaaatcaaa aatatcaaaa atgggaaaaa ttattataat aataaaaatg
    11401 aatgctaaaa agagagggaa aaactccctc tcatatgttc cgttatattg taaagattaa
    11461 aacgatcgca ataaccaggg aatatatacc agttgactct gctacagcct gacctaaaag
    11521 catagtagta ataatgttac ctcttgcttc cggatcgcgt gctacagatt cgactgcttt
    11581 accggcagca taaccctgac caataccagg accaataccg gcaatcatgg caaatccagc
    11641 tcctagtagg gcagcagctc taataatagt atcaataact tgtggtgaaa attctaccat
    11701 tttattttcc ccctttctca aatttgtcgt cttttaaatc tttatattct tctaattttt
    11761 tattcaatcc tcccctggcc atatcaagga ttgcccacat gatcactgta aacttcatca
    11821 tgaacagtcc tatggctgca gccagaaagt ttaaagaagg atttttcctg gcaactatta
    11881 atacaacaaa ataaatccca tattctataa agtatctgtt tctaataaag gattctgctt
    11941 taccttcctg catatcaagt gccttttgaa tattaatcgc ctttaatctt aacatcaaag
    12001 tacttattac cagaccgaaa ataaagccta ataaagtttc aaaattgcct gaaattaagg
    12061 agtataaaat aggtatactt gatattaaat acgttctttt tattatttcg atctgcattt
    12121 ggcgcggatc attaatatac ttcataactt aaacctcaaa actattttta agtgaaaacc
    12181 agataaatag caataactaa agagtaaata ccagtggaac ctgaaattat ctgtcctaag
    12241 agcatagtag taactatcgc tccccgtgct tccggctgcc ttctaactgc agaagtagct
    12301 ttaccagcag caaagcccat accgacgccg ggacctactc ccgctatcaa agccaggccg
    12361 gctgcgatta cagaaacact tcttattatc atttcaatag tttccggtga aaattctatc
    12421 ataaagtgtc ctcccttctg ccgaaaagaa aattcttacc ttaagatttt gctacagaga
    12481 tataagcaat ggcaaccata ccaaagatca aggcctgaat taaaccaata aagaaatcaa
    12541 accaggcatg gatgggtact gggattaaac ccggggctgc ctgataaatt aaagtgataa
    12601 tgattccacc gcctacgata ttaccaaaaa gacggaaaga gtgtgaaata ggtttcgcga
    12661 gttcacccac aacatttagg ggaagtaaaa agatgactgg ttgtccaaaa ctttttatgt
    12721 atccccagac tcctttttct ctcattcctt cgatatgact tgttaaaaat actactaagg
    12781 ctaagcctaa agtggtattc aagtcaccgg ttggactagc aactcccggt aataaaccga
    12841 ttaggttacc tacaccaaca tagatgaata tggtggcaat aaaaggtaaa aaccgccagc
    12901 cttctcccgg aagcatcggc tcaatctggt catgaattgc cgtgattaga aattctgcaa
    12961 tattctgttt tttacctggc cttattttta actcgctggt taaatatttg gccaagataa
    13021 taattaaaac aacagatacc caaccgacaa tcagggtatc agtaattggt aaatatgatt
    13081 ttccaaagag gtgggttacg acttttggac cgggattcat agcaaagtct cctttcttga
    13141 aaagttaatc ctgttcatca tcttcatcgt cttccataga acttaatatc agtttataat
    13201 ctgaataaaa accggatccg ataccgatga tgatacctaa aattttaaat aagtatgtat
    13261 atcctaacca ctggtctaac ttgagaccaa ggtagaagcc aactccaata ttaacaatga
    13321 taacaatacc aagttgggtt aataaaccca ttgttcgcat aattttacgc cagtcttgtg
    13381 gtttcatttt tattacctct gactgtacat atttttgcaa ctctacagag agtaattata
    13441 tcatagttaa tttccaattt ccaatgaaaa tcttaaaata tatctgcttt ttacgattta
    13501 atgtccataa ggaaaataat ataaaaacag ataaatacaa ccttggtttt aaggcaataa
    13561 tcatgattat attttcatcg tgattattgt taaatatatc acaacagtct gcaaatgtaa
    13621 agtatttagt tgctttcttc cagtgcttga ctgtcaggtg agagttcagt tgaaaagata
    13681 ccgagcttcc aggccccgta gataacaccg gttatagcca ggccaaatag tatcatacca
    13741 tttttaatat tgagtccgtt gataattaaa gctgtcgccc ccagcattaa actactacca
    13801 taaactatga taacagcctg tctgtgactc caacctaagg ctataagcct gtgatgtata
    13861 tggccccggt ctgcttcacc aattggcttg ttgtggtata cccttcttat gatcgcaaat
    13921 attgtatcaa aaataggtac acctaaagcc aaaatcggta caactatggt gacagctgct
    13981 gcactcttta aagcaccagt agcagatatt gccgccagga taaaaccggt aaacatcgag
    14041 cccgaatcac ccataaagat ttcggcggga tggaaattaa acttcaagaa accactgagg
    14101 ctgcctgcca aggcaatagc taaaacggcg gggattaaac ggtattcctg cattgcaact
    14161 gcaaaaaggg tcatagtagc gattattgag ataccggctg ccagaccatc tagtccgtca
    14221 attaaattaa ccgtattcgt gataccaacc acccataaga tggtaacggg aaaactaaag
    14281 tatcccaaat agatcatacc gcctaaagga ttggtaataa agttaatctg taccccgtaa
    14341 tagattaaga ttgaggccgc accaatttga cctaaaagtt ttacaagggg agaaatttca
    14401 aaaatatcat ctattaggcc taaaataaag ataaaggtgg ccccaaacat tatcccatag
    14461 gtagacaggg aatggtcatt aaaaaggaaa taacttatta taaaagcggt gaagatagct
    14521 atcccccctg tattgggaat aggcttggta ttaatgcgtc gtttagaagg atggtcaaac
    14581 ccattaataa cattagccat ttttttaacc aggggtgtta acagatagct tattattaaa
    14641 ctggatacaa aaatcaagat aaattttgat atcacttttt attcccttct ttgttaacag
    14701 ttattttgta ccgtaaagtc tatcaccggc atctcccagt ccgggaacta tgtaagcatg
    14761 gtcatttaac ttttcatcga tagcagcagt ataaatatct acatcaggat gttctttttg
    14821 caccctttca attccttccg gtgcagcaat taaaactact aacttgatat ttctagggtc
    14881 tctatctttc acaaaatcaa ttgcggcaca agcagaacct ccggtcgcca acatggggtc
    14941 taccacaagt aagtcccttt cagttacatc ggtgggtaat ttacagtaat attcaaccgg
    15001 ttctaaagta tctggatccc tatataatcc aacatggcct acttttgcag tcggaattaa
    15061 ctgcagtact ccatttaaca tgcctagtcc cgctcttaaa ataggcacta ttcctaattt
    15121 cttaccttct atcatcttaa aggtcccttt acagatcggt gtttcgacct caacatcttc
    15181 taagggtagg tctctggtta cctcatatac catgagtgtg gaaacttcgt caactaattc
    15241 tctaaattct ttcggtccag ttgtcttatc cctgattaaa gtcaatttgt gttggattaa
    15301 tgggtgctct ataagtattg tctcgctcat ttggttaata ttcgcccctt tcgctttctt
    15361 aaaatttagt ctggagtctt accaacaaat caaaggctac taaacaaagc tgattttaat
    15421 aaatttttaa cgctgtgcga ataagtaaga aagttaataa ttattaatag aggttaaaat
    15481 tgcctaaaaa tttttttctg ttaaaaaatt atacctatca tcctaacttt tagtattcta
    15541 gataagttaa ctttttcctg cctttttagc tattacttaa cttttttaac agctctttga
    15601 ctgcatattc gatctcatcc ctggtctttc tatacttgtc gatatc
//
LOCUS       DATGCJ010000129        16869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_373865, whole genome shotgun sequence.
ACCESSION   DATGCJ010000129 DATGCJ010000000
VERSION     DATGCJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373865"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VKN64_06495"
     CDS             complement(<1..234)
                     /locus_tag="VKN64_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMA59924.1"
                     /translation="MAKEKFERTKPHLNIGTIGHVDHGKTTLTAAITSVLSNYGGAEV
                     RDFDSIDNAPEEKERGITIATSHVEYETEKRHYA"
     gene            complement(312..956)
                     /gene="sigH"
                     /locus_tag="VKN64_06500"
     CDS             complement(312..956)
                     /gene="sigH"
                     /locus_tag="VKN64_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HMA59925.1"
                     /translation="MNTNAQEVEQQDYSQLSDDELIEIIHEGDSRAEEVLIKRYKNFV
                     LAKSRSYFLVGAGREDIVQEGMIGLYKAVRDYKIDRLASFRAFAELCITRQIITAIKA
                     ATRQKHQPLNSYVSLNKPIYDEESDRTLLDILKGTKLNNPEELFVSKETYDLIESQIS
                     EMLSDLEFNVLQEYLNGKSYQDIADTLDKHVKSVDNALQRVKRKLEEFLEKHDI"
     gene            complement(1019..1750)
                     /gene="rlmB"
                     /locus_tag="VKN64_06505"
     CDS             complement(1019..1750)
                     /gene="rlmB"
                     /locus_tag="VKN64_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMA59926.1"
                     /translation="MRNVEGKNPVIELIKEGKEVEKIYLQKGLRGDKIKLILEMAEEN
                     NIYLEWLPKFKIDEKAVTDNHQGVIALTSEIETYDLYEIIDSLENKDDPLILILDHIQ
                     DPHNFGAIIRTAHAAGVDAIIYPKDRACSITATVDKVSAGAIEHVNLVEVVNINRAID
                     VLKEKYYWIVGADINGEQNYFEHDFEGKYALVLGHEGSGLARLTKEKCDFLVKIPMQN
                     EFSSLNVSVAAGVILFEVNRQRNTI"
     gene            complement(1750..2454)
                     /gene="thyX"
                     /locus_tag="VKN64_06510"
     CDS             complement(1750..2454)
                     /gene="thyX"
                     /locus_tag="VKN64_06510"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HMA59927.1"
                     /translation="MEVKPKAELIDYTDNPERNVATAARLCYSKKGADRLNEEMSREQ
                     VENLVRKVVNMGHTSTLEHTYFFFHVQCSRVTSHQIVRQRIGTSYSQRSQRYVTEDNF
                     DYITPPKIKKNEEAYKKYQEKMEELEETYLEFIEMGVPKEDARFILPTIKTNIVISYN
                     ARSLMHFLRLRTCNRAQWEIRSIAWQMLNEVKKVAPVIFENAGPPCETDGECPEGDLS
                     CGKIDKIKAKKAEAKT"
     gene            complement(2466..3908)
                     /gene="cysS"
                     /locus_tag="VKN64_06515"
     CDS             complement(2466..3908)
                     /gene="cysS"
                     /locus_tag="VKN64_06515"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMA59928.1"
                     /translation="MLKVYNTLTREKEEFVPVEKNKVKMYVCGLTVQNYSHLGHMRGV
                     INYDVIRNYLEYKGYEVKYISNFTDINEKIIARAELEDMEPMALAHKYSKAFLEDLDN
                     LNIKRADHYCKATENIDNIIEMVQTLVDKGFAYVTDGNVYFSVEEFADYGKLSGRNLD
                     EMIAGTRKKVESDKKNPLDFALWKKVDDQDVMAWDSPWGRGWPGWHIECSAMSIQELG
                     STFDIHGGGTDLIFPHHENEIAQAEAYSGVKPFVKYWMHNGTVNMSGDKMSKSLGNFY
                     TTRELLEKYTADQLRYFILTKHYRSPIEYNEEEIESTNKSLEKLINTKKALDNILKGK
                     YDFVEEEVEVDLLDKVQKKEKEFFAAMDDDFNTALASGVINEIATEINKVINDSDFKL
                     SRELFKDLEKVYNIYTKLTDLFGLTLEGAQVEVESEKFNDLVEYILSLREEARENKNY
                     ELADKIRDDLAEMGIKVNDTPRGTEWEITN"
     gene            complement(3976..5442)
                     /gene="gltX"
                     /locus_tag="VKN64_06520"
     CDS             complement(3976..5442)
                     /gene="gltX"
                     /locus_tag="VKN64_06520"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMA59929.1"
                     /translation="MRLRFPPSPTGKIHIGNMRTALFNWLWARKNEGELVLRIEDTDQ
                     KRSTKEFEDIIIQELDWLGLDVDEGAGVGGDFGPYRQSERLGIYEKYAKKLLEEDKAY
                     KCYCTEEELDEMREKALEEGRMPKYNGHCRNLSEKEKQKYEEEGRDSVIRFKLPAEER
                     DIVVEDEIRGDVTFNTSVLDDFIIFKSDGMPTYNFAVVVDDALMEITEVIRGEDHLSN
                     TPKQILIYEALGFEVPKFAHLPLILDSDRAKLKKRSEDDDVYIGEYRAKGYLPEALFN
                     FLSFLGWAPPEDKEILSKEEIIENFELKDINKSAAVFDIDKLNWVNGKYIRESDLDYI
                     VELSIPYLKEAGYIDDQFVEDNYEWLKKVIDVSRTGVDYLAQIPEEAELFFKELEFED
                     KEQAIEEFKEEGVEVVFDHLKKKLKELDEFTPEKINNIFSELISELPVGGRTVYHPTR
                     LALTGLNSGPEMKHVIYILGVEETINRLDNALAMKANN"
     gene            complement(5510..6295)
                     /locus_tag="VKN64_06525"
     CDS             complement(5510..6295)
                     /locus_tag="VKN64_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HMA59930.1"
                     /translation="MLIKDIMTKNPITIKVDSTVMEAERVLSLNKIGRLIVVEDDDIV
                     GILTDGDLMNNDNIKEKIGKYMSEDVITIKELDTVQKAAKILSNNQIGGMPVVNKDDE
                     LVGIITADDIVYGYMKDEEELEVEKKNINPESAAIYLAMTRSREYEEYWLEKISGYGY
                     KGAITQTGANAEKLPVKLRESTTVAAIARGVISENSREKSAVSNAVKDAYSQLALINP
                     GLGGGFKIAVVRGPEYVSVAIFGKFGHALVDGPEQLAIGTSVI"
     gene            complement(6326..6799)
                     /gene="ispF"
                     /locus_tag="VKN64_06530"
     CDS             complement(6326..6799)
                     /gene="ispF"
                     /locus_tag="VKN64_06530"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HMA59931.1"
                     /translation="MRVGIGFDVHPFTEESPLYLGGIKIESDFGLQGHSDADVLIHAL
                     IDALLGAMGKGDIGELFPDSDQKYKNIRSSKLLDTVVEIMFNNNYTLINADLILLAEK
                     PKIKKYKNKIEKNLSEILKVDTTQINIKATTTEGLGFIGRVEGIGAQAVVLLENK"
     gene            complement(6801..7493)
                     /gene="ispD"
                     /locus_tag="VKN64_06535"
     CDS             complement(6801..7493)
                     /gene="ispD"
                     /locus_tag="VKN64_06535"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HMA59932.1"
                     /translation="MYITAVIPAAGMGSRMGIRENKLYLKLEDKPVLYHTLHNFVSNS
                     FIDKITLVLQENEMDRCEREILSLFKKGRQKIEMVKGGQSRKGSVFNGIKKAPKKTDY
                     IIIHDGARPLITQKIINNVIGALEKEEAVTTGVDVKDTIKVKDHNNYVVKTINRDNLT
                     SIQTPQAFSYELIKKAHQSDIYDKNITDDAYLIELMNKSVKIVEGSYENIKITTPIDI
                     TIAEEILKSRRE"
     gene            complement(7525..8613)
                     /locus_tag="VKN64_06540"
     CDS             complement(7525..8613)
                     /locus_tag="VKN64_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMA59933.1"
                     /translation="MLKKIVKFIFGIIGAVIGYNSVVMLGFAEEIEFTTSNIWSFLIS
                     KQFIGIIIGALIGYFLISYLLEKLLDFILNFDYKLKEFSWKKVIISFLGIILGLIIGS
                     LINLAFSISSIPKLGLPLQILINVIFIYLGLTLALYKSDDIFNLLFENKKFFAGSKNS
                     SITKKILDTSVIIDGRIYDICKTGFVEGKLMIPEFVLEELRHIADSSDGLKRNRGRRG
                     LDILKKMQKDNRIDVDIVSENYEEIPEVDSKLVKMAQEFEAKIVTNDFNLNKVSELQG
                     VFVLNINELANAVKPVVLPGEEMDVKVIKEGKEDRQGIGYLDDGTMIVVDNGIEYMDE
                     DISVMVTSILQTAAGRMIFAKPKSEGEY"
     gene            complement(8681..9187)
                     /locus_tag="VKN64_06545"
     CDS             complement(8681..9187)
                     /locus_tag="VKN64_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HMA59934.1"
                     /translation="MFEKGDKVVYPNHGAGTIVDIEVKEILGEEKKYYIMELPIGEMR
                     VMIPVHKVEEIGIRDVIDEEEAENVFHLLKGEKSKMSQNWNRRYRANMEKLKTGDIYE
                     VVEVVRNLTIRDMEKGLSTGEKKMLSNARKILISELVLAKDMSEEVVVEKIDNILENN
                     PNVNDDED"
     gene            complement(9214..10296)
                     /gene="disA"
                     /locus_tag="VKN64_06550"
     CDS             complement(9214..10296)
                     /gene="disA"
                     /locus_tag="VKN64_06550"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HMA59935.1"
                     /translation="MKDHEEWLNEVLELIAPGTQFREGLENILRAKTGGLIILTENDE
                     EIESLVDGGFKINAELTSTSLYELGKMDGAIVLDQDAENIIYANALLIPDPTISSSET
                     GARHRTAERVAKQTSNLVIAISERRDIITLYKEDKKHVLEDIRVVLSKANQAVQTLEK
                     YRKVFDQTLINLSALEFENLVTIADVVTVIKRAEMVLKIENEIERYILELGNEGRLIK
                     MQLEELVMNVEEENKLLIKDYISDEKLEEGITAEDVIEELNELNKDDMLSTREIRNVM
                     GHGGNVNSLDLLIEPRGYRILRKIPRLPMPVIENLVEEFGDLNEINVASLDELDEVEG
                     VGEVRAQAIKDGLRRLRDQVLLDRHI"
     gene            complement(10330..11700)
                     /gene="radA"
                     /locus_tag="VKN64_06555"
     CDS             complement(10330..11700)
                     /gene="radA"
                     /locus_tag="VKN64_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMA59936.1"
                     /translation="MAKNKKKYICENCGYKTENWMGKCINCGEWNSIKEYTPSEGQGG
                     NDFQAEEIKLPKIESLDQITGSQKSRFPTSLTELDRVLGGGIVEGSLILMGGSPGIGK
                     STLILQVAFLLANKNKNILYITGEESVNQVRMRAERLDTIHENIFILSEVDYQKIKAV
                     IEKRDFDLVIIDSIQTVKHPQIDSSAGSITQIKEVSSSLFRMAKETNLPIIIIGHITK
                     EGNLAGPKVLEHIVDTVLQMEGDQNYSYRILRGLKNRFGSTDEMGIFKMSSDGLEEVK
                     NPSQIFISERPENASGSIIVPIMDGNRVILIEIQALVTDTAYSTPQRLTTGINKKRLS
                     ILLAVLEKKLGFNFKASDVHVNITGGLYASEPAIDLGIVFSVISSFRDFPVDSNTAVL
                     GEVGLTGEVRAVNLFNKRIKELKKLGFKNIIVPSGNVQERMFDPELNLIEIKNLQEGL
                     DLYFNY"
     gene            complement(11814..14234)
                     /locus_tag="VKN64_06560"
     CDS             complement(11814..14234)
                     /locus_tag="VKN64_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMA59937.1"
                     /translation="MFGKFTERARRVIAMAESEAKKLNHNYVGTEHILLGLVKEKKGI
                     AGKVLSEEANLKENQIIDVIKSIIGKGKTKVDGTIGLTPRSKKVLNLSMEEARKLNHN
                     YIGTEHILLGLIAEGEGVAIRILQEVGVDLEKVKKEIMDILSDGSQNSAETEKSDTPE
                     LDKYSRDLTEMAAEDKLDPVIGREKEIERVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAKSITDENVPEILLDKRVVALDLSSLVAGSKYRGEFEQRLKALMSEIVESGNVILFI
                     DEMHTLVGAGAAEGAIDAANILKPALARGELQAIGATTLDEYRKYIEKDAALERRFQS
                     IMIEENTPEQSVEILKGLRDPYEAHHKVKITDEAIEAAVNLSHRYITDRFLPDKAIDL
                     MDEAASRVRLRNNTRPNELKELNERIEEVEKEKEAAVKNQEFEKAAELRDKEKELEKE
                     LEELQEEWQQTRGRNDMVVETEDIAQIVSSWTGIPVTKLEETETKKLLKLEEHLHERV
                     VGQDEAIKAVSQAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARTLAEAMFDDEDA
                     MIRIDMSEYMEKHAVSRLVGSPPGYVGHEEGGQLTEPVRRRPYSVILFDEIEKAHPEV
                     FNILLQILDDGALTDAQGRKVDFKNTVVIMTSNVGANYIKKQSQLGFTAEKDEESEYE
                     NMKENVISKLRDTFRPEFLNRIDETIVFHSLNKDHITKIIEIMIKELQERLDAKEIEI
                     DITKDAKMKLIDEGFDAEFGARPLRRAIQRMVENRLSEKILSGDIAEGDKVEVDEKDD
                     ELVFNKVE"
     gene            complement(14240..15313)
                     /locus_tag="VKN64_06565"
     CDS             complement(14240..15313)
                     /locus_tag="VKN64_06565"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HMA59938.1"
                     /translation="MFNDKNISLSNWIKNKGSKDDIILSSRIRLARNLKMLPFPRQSN
                     KQQLDKVVDIIKDILLDKELEIAGDKYKFNYIEMEKLSPVKKMMYVEKNLSSPHFVKN
                     DGYGKILFYDQDEKISIMVNEEDHLRIQILLPGLQIKKAWRIADQLDDILERDTEFAF
                     SKDWGYLSACPTNVGTGLRSSVMVHLPGLNLSNNINRLFEVVGKLGLTVRGIYGEGSE
                     SAGNIYQISNQITLGSKEEDIIENLESVTKQVITQEKNARNKLFKEREVFIKDEILRS
                     YGKLKYAYSISTSEAMELLSYVKLGMEMGVINEIDSLLLKRLFILIRPAHLQSLIGEE
                     VDSTKRDLKRAELIQTELNGKGG"
     gene            complement(15316..15822)
                     /locus_tag="VKN64_06570"
     CDS             complement(15316..15822)
                     /locus_tag="VKN64_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HMA59939.1"
                     /translation="MLCDKCKEKEATIHITKYVDGEKSEVYLCEQCAKETGHIDDNDV
                     FSFKNLIAGILNPNVEDYEEKSTKGLKCDNCGLTYEEFRREGRVGCPECYNTFEDKLR
                     PLIKRIQGSEKHVGKIPKDKDKYLRVKRDIQELKEEMEKVVADENFERAAEIRDEIYA
                     LEQKIGSE"
     gene            complement(15849..16307)
                     /locus_tag="VKN64_06575"
     CDS             complement(15849..16307)
                     /locus_tag="VKN64_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HMA59940.1"
                     /translation="MSSLSDKIEKYLRNLIQQYEGEIEIKRNRLADDFNCAPSQINYV
                     LDTRFTVERGFIIESRRGGGGYVRIIKVKLNSKNEAMKNIIKQLEGPLQPKEAKGIIK
                     RLHENNLIRQRDRVLLEAAISKESIGFENEEKNAIRAQVLKNTLKAIFKI"
     gene            complement(16736..16811)
                     /locus_tag="VKN64_06580"
     tRNA            complement(16736..16811)
                     /locus_tag="VKN64_06580"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16776..16778),aa:Asn,seq:gtt)
BASE COUNT         4714 a         3627 c         2288 g         6240 t
ORIGIN      
        1 agcatagtgt ctcttttcag tttcatactc aacgtgagag gtcgcaattg taatacctct
       61 ctctttttct tcgggagcgt tgtcgattga atcgaaatct ctaacttctg ctccaccata
      121 gttagaaagt acagaagtga ttgctgcagt taaagtagtc ttaccatggt caacgtgtcc
      181 aatagtaccg atattcaaat gtggtttcgt tctttcaaac ttttccttag ccattatatt
      241 catctctcct ttgttaatat tgtagcacag tgtgaataca ttttaatatt atataaatat
      301 tttaaaaaat tttagatgtc atgcttttct aaaaactctt ctaattttct tttaaccctc
      361 tgtagagcgt tgtctacaga cttaacatgt ttatccaaag tatcagcaat atcctgatag
      421 gatttaccat tcagatattc ctgtaaaaca ttaaattcta aatcactaag catttcacta
      481 atttggctct caattaaatc atatgtctct ttgcttacaa agagttcctc gggattattc
      541 agttttgtcc cttttaaaat atcaaggagt gtgcggtctg attcttcatc gtaaattggc
      601 ttatttaagg atacatatga atttaacggt tggtgtttct gcctagtggc tgccttaatt
      661 gctgtgatta tctgtctggt aatacacaat tctgcaaaag ccctaaaaga agctagacga
      721 tcaattttgt aatctctaac agctttatat aggcctatca tgccttcctg tacgatgtct
      781 tctctacctg ccccaacaag aaaatatgat cttgatttag ccagcacaaa attcttgtac
      841 ctttttatta atacctcttc tgctctggag tctccttcgt gaattatttc tattaattca
      901 tcatcactga gctggctgta gtcctgttgc tcaacttctt gggcatttgt gttcaaccag
      961 aatcccccct tatttaatac attaatatta tactgtttaa aactttttta gtcaaggatt
     1021 atattgtatt cctctgccga ttgacctcaa ataatattac accggccgca acagatacat
     1081 taagtgaact aaattcattt tgcatcggta tttttactaa aaagtcacac ttttctttgg
     1141 ttaaacgggc cagaccactt ccttcatgtc ctagtactaa ggcatatttg ccttcaaaat
     1201 catgttcaaa atagttttgt tcaccgttaa tatctgctcc tactatccaa taatattttt
     1261 ctttaagaac atcaatagct ctattgatat tgacaacttc cactaaattt acatgttcaa
     1321 tcgcaccggc tgaaacctta tccactgttg cagtaatact acaggcccta tctttgggat
     1381 aaataatggc atcaactccg gctgcatgag cagttctgat tattgctcca aaattatgtg
     1441 gatcctgaat atggtctaaa attaaaatta aaggatcgtc tttattttct aaagaatcta
     1501 ttatttcata taaatcatat gtttcaattt cactggttaa ggcgattaca ccctggtggt
     1561 tatcagtaac tgccttttca tctatcttaa actttggcag ccactccagg tagatattat
     1621 tttcctcagc catctcaaga attagcttaa ttttatcgcc tcttaaaccc ttttgcaagt
     1681 atattttttc aacctctttg ccttctttga ttaactcgat aacaggattt ttaccttcaa
     1741 catttctcat tatgtttttg cctcagcttt ttttgcttta attttgtcta ttttgccaca
     1801 ggataaatcg ccttccggac actcaccgtc tgtttcacag ggaggaccgg cattttcaaa
     1861 tatgacagga gcaaccttct tcacttcatt taacatctgc caggcgatgc tcctaatttc
     1921 ccattgcgcc ctattacagg ttctaagtct cagaaagtgc attaatgaac gggcattata
     1981 tgaaatcact atattagttt tgatggtagg aaggataaat ctggcatcct ctttgggaac
     2041 acccatctcg ataaattcta aataggtttc ttccaactct tccatcttct cttgatactt
     2101 tttataagct tcttcattct ttttgatctt gggcggagtt atataatcaa agttatcttc
     2161 tgtcacatac cgctgggatc tctgagaata agaagtccca attctttgtc tgacaatttg
     2221 atgagaggta acccgactgc attgaacgtg aaagaaaaaa tatgtgtgtt ctaatgtaga
     2281 agtatggccc atatttacta cttttcttac caggttttca acctgttctc ttgacatctc
     2341 ttcatttaat ctgtcagccc cttttttgga ataacataag cgggctgccg tagcaacatt
     2401 tctttccgga ttatctgtat agtctattaa ctctgctttc ggttttactt ccattttact
     2461 cccccttagt ttgtaatctc ccactccgtt cctcgcgggg tatcattaac ttttattccc
     2521 atttcggcta gatcatctct aattttatct gctaattcat agtttttatt ctcgcgggct
     2581 tcttctctta aggataaaat atactcaacc aaatcattaa acttctccga ttcaacttct
     2641 acctgagctc cttctaaagt taaaccaaac aaatcagtta atttagtata tatattgtag
     2701 accttctcta gatctttgaa caattctctg cttaatttaa aatcagaatc attaattact
     2761 ttatttattt cagttgctat ttcattaata acacctgatg ctaaggctgt attaaagtca
     2821 tcatccattg ctgcaaaaaa ttctttttct tttttctgta ctttatctaa taaatcaact
     2881 tcaacttctt cttcgacaaa atcatacttt ccttttaata tattatccaa tgcttttttt
     2941 gtattaatta atttttctaa acttttattg gtgctttcga tttcttcttc attatattcg
     3001 attggactgc ggtaatgttt agttaaaatg aaatatctta actggtcagc cgtatatttt
     3061 tctaaaagct ctctggtggt ataaaagttg ccgagtgatt ttgacatttt gtctccactc
     3121 atgttcaccg taccattgtg catccagtac ttcacaaatg gttttacacc actataggcc
     3181 tctgcctggg cgatttcatt ttcatgatga ggaaagatta aatctgttcc acccccatgg
     3241 atatcaaagg tactgcccaa ttcctggatg gacatggcag aacactctat atgccaaccc
     3301 ggccagccgc gtccccaggg gctgtcccat gccattacat cttggtcatc tacttttttc
     3361 cagagggcaa aatcaagtgg attcttttta tcactttcca ctttttttct ggtaccagca
     3421 atcatctcat ctaaatttcg gccggaaagc tttccatagt ctgcaaactc ttcaacacta
     3481 aaatagacat tgccgtctgt tacataagca aaacctttat caactaacgt ttggaccatt
     3541 tctataatgt tatctatatt ctcggtcgcc ttgcagtaat ggtcagccct tttgatattt
     3601 aaattatcga gatcttctaa aaaagctttg gaatacttat gggctaacgc cattggttcc
     3661 atatcttcta gctcagccct ggcaattatc ttttcattaa tatctgtaaa gtttgaaata
     3721 tattttactt cataaccctt atattcaagg taattacgaa ttacatcata attgataaca
     3781 cctctcatat gccccagatg agagtagttt tgtacagtca atccacatac atacatcttt
     3841 accttgtttt tttcgaccgg cacaaattct tctttctctc gggttaaagt attatagact
     3901 tttagcacat gggacactcc tttcttgtga aaaacttctc ccccaatcaa ggaggagaag
     3961 tccctttatt tttatttagt tgttggcttt catggctagc gcattgtcaa gcctatttat
     4021 tgtctcttct acacccagaa tatatatgac atgtttcatt tccggtccgg aattaagacc
     4081 ggttaacgct aatcttgtcg ggtgatagac ggttctaccg cctacaggta gttcacttat
     4141 taattcagag aagatattat tgatcttttc aggggtaaat tcatccagct cttttagctt
     4201 tttcttgagg tgatcaaaaa caacttcaac cccttcttct ttaaattcct cgatagcctg
     4261 ttctttatct tcaaactcta attccttaaa gaaaagttca gcttcctcgg gaatttgagc
     4321 tagataatca acaccggtcc gcgatacatc aatcaccttt ttaagccatt catagttgtc
     4381 ttctacaaat tgatcatcaa tatagccggc ttcttttaaa tatgggatcg atagttcaac
     4441 aatataatct agatcagact ctctgatata cttaccatta acccagttta atttatctat
     4501 atcaaataca gctgcacttt tattaatatc ctttaattca aagttttcaa taatttcttc
     4561 cttgcttaaa atttctttat cttccggtgg agcccagcct aaaaaagaaa ggaagttaaa
     4621 taaagcttcc ggtagataac ctttggctct atattcgcca atatagacat catcatcttc
     4681 acttctcttt ttcaacttgg ctctatcact atcaagtatt aagggaagat gggcgaattt
     4741 cggcacctca aatcctaagg cttcatatat taaaatttgt tttggggtat tagacaaatg
     4801 gtcttctccc ctgataactt ctgtgatttc cattaaggca tcatccacca caacggcaaa
     4861 gttataggtg ggcattccat ccgatttaaa gatgataaag tcatctaaga cagaggtgtt
     4921 aaaggttaca tcacctctta tctcatcttc taccactata tcgcgttcct cggctggcag
     4981 tttaaatctt atgacagaat ctctgccttc ttcttcgtac ttttgttttt ctttttcaga
     5041 tagattgcgg cagtgcccat tatatttagg cattctacct tcttctaaag ccttttcacg
     5101 catttcatct aattcttctt ctgtacagta acatttatat gctttatctt cttcaagtaa
     5161 cttttttgca tatttttcat agatgccaag tctttctgac tgtcgataag gtccaaagtc
     5221 cccaccgact ccggcccctt catctacatc gagacctaac caatcaagtt cctgaataat
     5281 tatatcttca aattcttttg ttgatctttt ttggtctgta tcttcaattc ttaagacaag
     5341 ctccccctca ttttttctgg cccagagcca gttaaaaagc gctgtcctca tattaccaat
     5401 atgaattttt ccggtcggag aggggggaaa cctcagtctc atatcactca cggttaattc
     5461 ctccttaatt gttatgatca ttattgcatt tatttaagtt tattatctct tatattacac
     5521 ttgtaccgat tgctagctgt tctggtccat ctactaaagc atgaccaaac ttaccaaaaa
     5581 tagccacaga tacatattcc ggtcctctta ctacagcaat tttaaaaccg ccgccgagac
     5641 cgggattaat taaggctaac tgactatatg catctttaac tgcattggac acagctgatt
     5701 tttcacggga gttttcagat attactcctc tggcaatcgc tgctacagtt gtactttctc
     5761 ttagtttaac cggtaatttt tctgcattag caccggtctg ggttattgca cctttgtaac
     5821 cataaccact tatcttttca agccaatatt cttcatattc acggctgcgg gtcatcgcta
     5881 aatagatagc tgcactttcc gggttaatat tctttttctc aacctctaat tcttcttcat
     5941 ctttcatata accatataca atatcatcag cagtaattat accgactaat tcatcatctt
     6001 tattaacaac cggcatcccg ccaatctggt tgttcgataa aatcttagct gctttttgta
     6061 cggtatcaag ttctttaatc gtaattacat cttctgacat atactttcca atcttttctt
     6121 ttatattatc attattcatt aaatctccat ctgttaaaat accaactata tcatcatctt
     6181 caactacaat aagtctacca attttattta atgataatac tctttctgct tccattacgg
     6241 ttgaatccac tttaattgtt ataggattct ttgtcataat atctttaatt aacattattt
     6301 cttcaccttc ctcaatacta gataattatt tgttttctaa tagtactact gcttgtgcac
     6361 ctatcccttc aactctcccg ataaatccca gtccttccgt tgtcgtagcc ttaatattta
     6421 tttgtgtggt atctactttt aatatttcgc ttaaattttt ttctattttg tttttatatt
     6481 tttttatctt tggtttttca gctaaaagaa ttaggtctgc attgattaat gtgtagttat
     6541 tattaaacat tatttctaca acagtgtcaa gtaatttact gctgcgaata tttttatatt
     6601 tttggtcgct atccgggaaa agttctccaa tatcaccttt tcccatagca cccagtagag
     6661 catcaattag agcatgaatt aaaacatctg catccgaatg tccttgcaag ccaaagtcag
     6721 actctatttt aattccaccc agatataaag gactttcttc tgtaaaggga tgcacatcaa
     6781 aacctattcc tactctcatc ttattctctc ctacttttta atatctcttc agcaatagta
     6841 atatcaattg gggtcgtaat cttaatattt tcatacgaac cctctactat tttaacactt
     6901 ttgttcatta attctattaa ataggcatca tctgtaatat ttttatcata aatatcactc
     6961 tgatgagctt ttttaatcaa ttcataacta aaagcctggg gggtctgaat agaagttaag
     7021 ttatccctat ttatcgtttt aactacataa ttattgtgat ctttcacctt gatagtgtct
     7081 tttacatcga cacctgtggt aacggcctct tctttttcta aagccccaat cacattattg
     7141 ataatttttt gggtgattaa aggccgggca ccatcatgta taattatgta atcagttttc
     7201 ttaggggcct tttttatacc attaaaaacc gatccttttc tactctgacc gccttttacc
     7261 atctcaatct tttgcctgcc tttcttaaaa agagataata tctctctttc acaacggtcc
     7321 atctcatttt cctgcaagac caaagtaatc ttatctataa aagaattgct gacaaaatta
     7381 tgaagagtgt ggtaaagtac aggtttatct tccaatttga ggtataattt attttctctt
     7441 atccccatcc ggctgcccat cccggcagct ggaattactg cagttatata catattaaat
     7501 ctccttttag attaaaaata gattttaata ttcaccttca gatttgggtt tggcaaaaat
     7561 catacggccg gcagcagtct gtaagatact ggtaaccata actgaaatat cttcatccat
     7621 gtactcaatt ccgttatcaa ctacaatcat tgtaccgtca tctaaataac cgataccctg
     7681 cctatcttct ttaccttctt taataacttt aacatccatt tcttccccgg gaagtacgac
     7741 tggtttaaca gcattggcca gttcattaat gtttaataca aatactccct gcagttcaga
     7801 aactttattt aaattaaaat catttgtaac tatttttgct tcaaactcct gggccatttt
     7861 taccaacttg ctgtcaactt ccggaatctc ttcataattc tctgatacaa tatcgacatc
     7921 tatccggttg tccttttgca ttttctttaa aatatcgaga cctcgacggc cgcgatttct
     7981 cttcaatcca tctgatgaat cagcaatatg tcttaattct tctaagacaa attcagggat
     8041 cattaattta ccttctacaa atccggtttt acaaatatca taaatcctcc catcaataat
     8101 tacacttgta tctaaaatct ttttagttat actgctgttt ttactgcctg caaaaaactt
     8161 cttgttttca aacaataagt taaaaatatc atcagattta taaagggcca gggttaaacc
     8221 taaatatata aaaataacat taattaaaat ttgtaaaggc aaacccagct tgggaatcga
     8281 agaaatagaa aaagcaagat taataagaga accaattatt agtccgagta taatacctag
     8341 aaaagaaata ataacttttt tccaggaaaa ttcttttaac ttataatcaa aattcaaaat
     8401 aaagtcaagc aacttttcta gtaagtaaga tattaaaaaa taacctataa gagcacctat
     8461 aataattcca ataaattgtt tgctaattag aaaagaccat atattactgg tagtaaattc
     8521 gatttcttct gcaaagccca acattacaac actattatat ccaataacag caccaataat
     8581 tccaaaaata aatttcacta tcttcttcag cattcaatat cacctcctta tcctgttacc
     8641 ttatttaatt atcatctttt ttaaaaacca cctctctttt ttaatcctca tcatcattta
     8701 cattgggatt attttctaat atgttatcta tcttttcaac aacgacttct tctgacatgt
     8761 ccttagctaa taccaattca ctgattaaaa tcttacgggc attacttaac atcttctttt
     8821 caccggtaga tagacctttt tccatatccc taatggttaa gttccttact acctcaacta
     8881 cttcataaat atcaccggtt tttaatttct ccatattcgc tctatatctt ctattccagt
     8941 tttgagacat cttagacttt tcacctttta aaaggtgaaa gacattttca gcctcttctt
     9001 cgtcaattac atctctaata cctatttctt caactttatg aaccgggatc ataactctca
     9061 tttctccaat tggtaattcc ataatatagt acttcttttc ttcaccgaga atttctttta
     9121 cttctatatc gacaattgta ccggctccat gatttgggta aactacttta tctccttttt
     9181 caaacatatt atcaaccttc ctttataatc attttagata tgtctatcta ataagacttg
     9241 atctcttaac cgcctgagac catctttaat agcctgggct ctcacttccc caacaccctc
     9301 tacctcatct aattcatcaa gggaagctac attaatctca tttaagtcac cgaactcttc
     9361 cactaaattt tctattacgg gcattggtaa acgggggatt ttccttaaaa tcctgtatcc
     9421 gcgtggttca attaatagat caagtgaatt cacattacca ccatgtccca ttacattcct
     9481 gatttccctg gtagatagca tgtcatcttt atttagttca tttaactctt caattacatc
     9541 ttcggcagtt atgccttcct ccagcttttc gtctgaaata tagtctttga taagtaattt
     9601 attttcttct tctacattca ttaccaattc ttctaactgc atttttatca gtcggccttc
     9661 attacctagt tctaatatat atcgttcaat ttcattttct attttaagaa ccatttcagc
     9721 ccttttaatc accgttacca catctgctat tgtaactaaa ttttcaaatt caagggcact
     9781 taaattaatt aaggtctggt caaacacctt tctatatttt tcaagggtct ggactgcctg
     9841 gttagcttta gaaaggacca ctctgatgtc ttccagtaca tgttttttat cctctttata
     9901 tagtgttatg atatctcttc tctctgaaat ggcaattact aaattagagg tctgtttggc
     9961 aaccctttca gctgttctat gtcttgcccc agtttcactg gaactgattg taggatcagg
    10021 gattaataat gcattggcat aaataatatt ttcagcatct tgatccagaa cgatcgctcc
    10081 atccattttg ccaagttcat aaagactggt cgaggttaat tcagcattaa ttttaaatcc
    10141 accatcaacg agactttcta tctcctcgtc attttctgtt aatattatta aacctcccgt
    10201 ttttgctctt aatatatttt ccagtccttc tctaaactgt gtaccaggag ctattaactc
    10261 taatacttca tttaaccatt cttcatgatc tttcacaggg cagcctcctt tattttgata
    10321 attttataac taatagttaa aatataaatc taaaccttcc tgtaagtttt ttatctcaat
    10381 taaatttaat tcggggtcaa acatcctctc ttgaacattc ccagaaggaa ctattatatt
    10441 tttaaagcct aattttttta gttctttaat tcttttatta aacaggttaa cagccctaac
    10501 ttctcctgtt aaaccgactt ctcctaagac agccgtatta gaatctacgg ggaaatctct
    10561 aaaacttgag ataacggaaa agactatccc taaatctata gcgggttcac tggcatataa
    10621 acctcccgtg atattgacat gtacatcact tgctttaaaa ttaaaaccga gcttcttttc
    10681 aagcacggct aaaagaattg atagcctttt tttgttaata ccagtagtta aacgctgggg
    10741 agtagaataa gctgtatcag tcactaaagc ctgtatctct attaaaataa ctctattacc
    10801 gtccatgatt ggtactataa tagagccgct tgcattttcc ggtctttcac tgataaagat
    10861 ctgggaagga tttttaactt cctctaaacc atcggaactc attttaaaaa ttcccatctc
    10921 atcagttgaa ccaaatctat tttttaaacc tctcaaaatg cggtaagaat aattttgatc
    10981 tccttccatt tgcagcacag tatccacaat atgttctaaa actttaggtc cggctagatt
    11041 accctctttt gtaatatggc caattattat aatcggtaaa ttagtttcct tagccattct
    11101 gaacaaacta gaagaaactt ctttaatctg ggtaatactg ccggccgaag aatcaatttg
    11161 cgggtgttta accgtttgaa tggaatcaat gatgacaaga tcgaaatctc ttttctcgat
    11221 cactgccttg attttttgat aatcaacttc agataaaata aaaatatttt catggattgt
    11281 atcgagcctc tcagctctca tccttacctg attgacagat tcttcgcctg taatatagag
    11341 tatattctta tttttatttg ccaaaaggaa agccacctgc aaaatcaagg tggatttacc
    11401 aatacccgga gaacctccca ttaaaatcaa ggatccttct acaattcccc cacctaatac
    11461 tctatccagc tctgttaaag aagtagggaa acgactcttt tgggatcccg taatttgatc
    11521 aagagattca attttgggta actttatttc ttccgcttga aaatcattac ctccctggcc
    11581 ttctgaagga gtatactctt tgattgaatt ccattcaccg caatttatac atttgcccat
    11641 ccaattttca gttttataac cacagttctc acagatatac ttctttttat tttttgccat
    11701 tattactctc caattatata gacacaagat tgtttaatta tatataatta taccattttt
    11761 aagcagtttt tacaagattg aaacccagcc tctcagctgg gttcatttct agcttattca
    11821 actttattaa atactaattc atcatctttt tcatctacct ctactttgtc tccttcagca
    11881 atatctccgc ttaatatttt ctcagataag cggttttcta ccattctctg gatcgccctt
    11941 ctaaggggtc tagctccaaa ttctgcatca aacccttcgt ctattaattt catcttggca
    12001 tctttagtga tatctatttc aatttctttg gcatccagtc tttcctgtag ttctttaatc
    12061 attatctcaa ttatcttggt aatatggtct ttattgagag aatgaaagac aatggtttca
    12121 tctattctat ttaaaaattc aggtctgaag gtatccctta atttagatat tacattttcc
    12181 ttcatgtttt catattcaga ctcttcatct ttttcagctg taaagccgag ctgtgactgc
    12241 tttttaatat agtttgctcc cacattggaa gtcataatta ctacagtatt tttaaagtcg
    12301 acctttctac cctgcgcatc ggttaaggca ccatcatcta agatctggag taatatatta
    12361 aatacttccg ggtgagcttt ttctatttca tcaaagagaa ttactgaata aggtcttctt
    12421 ctcaccggtt ctgtcaactg ccccccttct tcatgaccga cataaccggg aggtgaaccg
    12481 actaatctag aaacagcatg tttttccata tactctgaca tatcaattct tatcatggca
    12541 tcttcatcat caaacatcgc ttctgctaat gttcgggcta attcagtctt acctaccccg
    12601 gttgggccta agaagataaa ggaaccaatc ggccttttgg ggtctttaag accggctctt
    12661 gcccttctta ctgcctgtga tacggctttg attgcctcat cctgtccaac cacgcgttca
    12721 tgtaagtgtt cttctaattt aagcagtttt ttggtttctg tttcttctaa tttggtgaca
    12781 ggtataccgg tccagctgga aacaatttgc gcaatatctt cggtctctac taccatatca
    12841 ttgcggcccc ttgtctgctg ccattcttcc tgcagctctt ctaactcttt ttccagctct
    12901 ttttctttat ctctaagttc agctgctttc tcgaactcct gatttttaac agccgcttct
    12961 ttttcttttt ctacttcttc tattctttca tttaattcct tcaattcatt aggacgggta
    13021 ttatttctta acctcacccg cgaagctgcc tcatccatta aatcaattgc tttatccggt
    13081 aaaaacctat cagtaatata cctatgagaa aggttaacag ctgcttcgat agcttcatcg
    13141 gttattttaa ctttatggtg tgcttcataa ggatctctta atccttttaa gatttccacg
    13201 gactgttcag gcgtattttc ttcaatcatg atagattgaa atctgcgttc taaagctgca
    13261 tctttttcaa tatattttct gtattcatct agtgtagttg caccgatcgc ctgtaattcg
    13321 cctctggcta gagcaggttt taagatattg gcagcatcaa ttgcaccttc ggctgctcct
    13381 gccccgacga gagtgtgcat ttcatcgata aataggataa cattaccgct ttccactatc
    13441 tcagacatca aagcctttag tcgttgttca aactctcctc tatatttaga accagctact
    13501 aaagaactca aatcaagagc tactaccctt ttatccaaga gtatctctgg tacattttca
    13561 tctgtaatac tttttgccag tccttctaca attgcggttt taccaacacc aggctcaccg
    13621 attaaaaccg gattattctt ggttctcctg ctaagcacct ggataactcg ttcaatttct
    13681 ttttctctcc cgatcacggg atcaagttta tcttcagccg ccatctcggt taaatcacgg
    13741 ctgtatttat ctaattcggg tgtatcgctt ttttcggttt cagcactatt ttgagaacca
    13801 tcacttaata tatccattat ttctttcttc actttctcta ggtctactcc aacttcctgg
    13861 agaattctaa tagctacacc ttctccttct gcaattaaac ctaacaagat gtgttctgtg
    13921 ccaatatagt tatggtttaa cttcctggct tcttccattg aaagatttaa tactttcttg
    13981 ctgcgggggg ttaagccgat tgtaccatcc acttttgttt tacctttgcc tataatggat
    14041 ttaattacat ctataatctg gttctctttt agatttgctt cttcggagag aaccttacct
    14101 gcaattccct ttttttcttt tactaaacca agcagaatat gctcggttcc aacataatta
    14161 tgattcaatt tttttgcttc agattcggcc attgctatga ctcgcctggc ccgttctgta
    14221 aatttaccaa acattatatt tagcctcctt tgccatttag ttctgtctgt attagctcag
    14281 ctctttttag atctcttttg gtagaatcaa cttcttctcc tattaaagac tgtagatggg
    14341 ccggtctgat caggataaat agtctcttta aaagtaaaga atctatctca tttattactc
    14401 ccatctccat ccctaattta acataggaaa gtaattccat ggcttcactt gtagatatac
    14461 tatatgcata ttttaattta ccataagatc ttaatatttc atcttttata aaaacttctc
    14521 tttctttgaa cagtttattt ctggcatttt tttcttgagt tataacctgt tttgttacac
    14581 tttctaaatt ttctataata tcttcttcct tactcccgag ggtgatctgg ttagaaattt
    14641 ggtagatatt accggcagat tcactgcctt ctccataaat accccggaca gtcagtccta
    14701 atttaccaac aacttcaaaa agcctattta tattattgct taaatttaat cccggtaaat
    14761 ggaccatgac tgaggatctt aagccagtcc ccacattagt aggacaggct gataggtaac
    14821 cccaatcttt ggaaaaagcg aattctgtat ctcgttctaa aatatcatca agttgatcag
    14881 ctattcgcca ggcttttttg atctgtaagc ccggtaaaag gatctgaatc cttaaatggt
    14941 cttcttcatt aaccataata ctgatctttt cgtcctggtc ataaaatagg atcttaccat
    15001 agccgtcatt ttttacaaaa tgagggctgc ttaaattttt ttctacatac atcatctttt
    15061 ttaccgggga tagtttttcc atttcaatat aattgaactt atatttatca ccggcaattt
    15121 ctaattcttt atctaataag atatctttaa taatatcaac gactttatct aactgttgtt
    15181 tattagattg tctgggaaag ggcagcatct ttaaatttct ggccaatcta attctgctac
    15241 ttaggattat atcatctttg cttcccttgt tttttatcca gttactcaat gatatgtttt
    15301 tatcattgaa catggttatt cactccctat tttttgctcc agagcataaa tttcgtctct
    15361 aatttcagca gctctttcaa agttttcatc tgcaactact ttttccatct cttcttttaa
    15421 ttcctgtata tctcgtttaa ctcttagata tttatcctta tctttgggta ttttacctac
    15481 gtgtttttca cttccttgaa ttcttttgat caatggccgc aatttatctt caaaagtgtt
    15541 ataacattca ggacaaccaa ctctaccttc tcttctgaat tcttcataag taaggccaca
    15601 gttatcacac tttaatccct ttgtgctttt ttcttcataa tcttctacat taggatttaa
    15661 aatacctgca atcaaattct tgaaggaaaa tacgtcatta tcatctatat gtccagtctc
    15721 cttcgcacac tgttcacaca aatatacttc tgatttctca ccatccacat atttggtaat
    15781 gtgaattgta gcttcttttt ccttacattt atcacaaagc atataaatcc ccctttctat
    15841 cccttacatt aaatcttgaa aatagctttt agtgtatttt taagtacctg agctcttatg
    15901 gcattttttt cttcattttc aaaaccaata gactctttac taatagccgc ttccaggaga
    15961 actcgatctc tttgtctaat taaattgttc tcatgtagtc tttttattat accttttgct
    16021 tccttcggtt gtaaaggtcc ttctaactgt tttataatat tcttcatcgc ctcgttttta
    16081 gaattcaatt taacttttat aatacgaacg tatcctccgc cacctctacg actttcaata
    16141 atgaagcctc tttcaacggt aaaacgggta tctaatacat aatttatctg ggaaggagca
    16201 cagttaaaat cgtctgcaag tctattacgc ttgatttcaa tttcaccttc atactgttga
    16261 atcaagttac gtaaatattt ttctatcttg tcagaaagac tagacattat agatcccctc
    16321 ttcactttga ctttttttga ccttctaata atataatact atattcatat caaaattcaa
    16381 ggggttattt aatattttta aaaaatttaa aaaaaccgcc acaaagtgac ggctttaatt
    16441 aattgcttag attgagactg tatattattt taatgaaaat caagataata attattatta
    16501 ccattatgat agcaagaata tcaatttctc tataaagtga aaatatacca agaaatagta
    16561 ataaagaacc taaaagcata atacttttag cagctttatt ataatcttta tgacttttta
    16621 atttaaaagg aagtatctta tgtttcccct tccaaattaa aaaactataa attaaaaccg
    16681 ttattccaat aacaattaac atatgtaaac accttttttg tgtatcaaat aaaaatggct
    16741 ccccgagcag gactcgaacc tgcggctcga tggttaacag ccatctgctc taccagctga
    16801 gctatcgggg agcgtataca ttaaataaaa aagattccgg cagtgaccta ctctcccaca
    16861 gggtcaccc
//
LOCUS       DATGCJ010000130        12749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_418473, whole genome shotgun sequence.
ACCESSION   DATGCJ010000130 DATGCJ010000000
VERSION     DATGCJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418473"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VKN64_06585"
     CDS             complement(<1..1341)
                     /locus_tag="VKN64_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HMA59941.1"
                     /translation="MERTKQNDALKFYGGKYMVFVPILLALVGLVYIALQNANIPEYW
                     VVFLLPLIFIMPFVKDKEKFANVIIDGFSSRVAMILVAAVMLAGISGTILNESGLVKT
                     LATYLVNMNLVGSKFVVATFILTGIVALSTGTSVGTIFVVSPILYPVGYLVGSNPALL
                     IGAIVSGGAFGDNLSPVSDTTIASATTQGMDIGGVVKSRLKYSIPTALITIVLYSLIG
                     RGGSLSDQISEYVGTPEPLSLLFLLVPIVIVYLCVRQHHLIEALSYGIITGLVLGLLT
                     GIITPNMIINVPEPFGAGGIILEGIQGSVPTIIVVLILFAQINFLKEGGGIDIIINMM
                     AKFVRGVRTAELSIVSLLISLNVLTGLNTAAIIGTGEIANEMGEKYSISGYRRANLMD
                     CAGTTLNYLIPYMVPVVVGSMMSSMYAPFEDAVQVSTVEVVTNQFYPWVMLAVLI"
     gene            complement(1373..3271)
                     /locus_tag="VKN64_06590"
     CDS             complement(1373..3271)
                     /locus_tag="VKN64_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HMA59942.1"
                     /translation="MNYGYCNQALRINLSNNTIEKETLDPQFLKAYLGGRGLNVRRLY
                     EEVPPEVDPLSEDNKMFIGVGPLTGTLFPGAARVNFTAKSPQTNILGDSNTGGFLGPE
                     IKFAGFDQIILEGKADQLSYIYIKDGKAEIRSAKHLKQLDTFETQEKIKEELGDSRVQ
                     IAAIGPAAENGVKYAGIFCNMVRAAARTGMGLVLASKNIKAIAVRGTRDLGAKKPDKF
                     KTLIDKMNQEIKNHSEYEIRTRLGTTQLVSSLNELGCLATRHFQTGYFEAGDKVSGEY
                     LEENYKQKEKGCFACTIPCSRFFEIKEGKYKGLKSEGPEFEGLAGFSSRVGVSDIEDA
                     LHGVDLCNRYGLDVITTSECIAFTMELYEKGILSSEEVDGLKPNFGNSEAVFALIEKI
                     KNREGFGNLLADGVVKAAERIGKDTKKLAMHVKGLEIFQADPRGIKGYGLGVAVATRG
                     GDHLRSEPWFEFSQNAAEGKRRFGHPESAFRLEYKGKGRVVKFYEERCVLADCLEACK
                     NTVVNMEIISFKEAAELLNALTEYDFTVEEIQKSAERIMNVERAYIVREGIRREDDSL
                     PDRFLNEPLPEECGESAGSIVELEPMLDEYYKARGWEVATGLPTENKLRELGLADIVI
                     DLKERNII"
     gene            complement(3271..3993)
                     /locus_tag="VKN64_06595"
     CDS             complement(3271..3993)
                     /locus_tag="VKN64_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HMA59943.1"
                     /translation="MYDLKIVVEEIEGFCDLPMEVGDYFTVSGGQIEIPEGKHMCLWA
                     MQSLMPVLPAKQREIKEENDWIPHTKRFICPDPNGGVVFRIDRIDPKTGKVVDKEKEI
                     PPRIQVDPEACTGCRACETVCAFVHSQSFNAKNSRMKIEKNERAGEDIPYVCRQCGDA
                     PCIKACPVDALSRDPATNAVLVDDDKCIGCFKCAEACPFDAITFTDDDTALICNLCGG
                     EVECVKRCPTDALKFDNKGGKE"
     misc_binding    complement(4076..4343)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4785..6437
                     /locus_tag="VKN64_06600"
     CDS             4785..6437
                     /locus_tag="VKN64_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA59944.1"
                     /translation="MRRKIRFMLSFVLILVLILPIFSQTVFGAEGDRVVDVTLLTRPK
                     SSASNEYEAINLVAKNLRKIGVQAEVKVTPWEQMADVVWYQRESWDITGWQMTARPER
                     LDPDEFIYNLFHSSTAEKGYNFIGYKNPQYDKIAEKQRVTIDREKRKELIYKAQKILA
                     EDVAYIYSVHPKINNVYNNQVFADDSMKEMAGLGLKNFWTYINAKPISDQKDIVLNFN
                     DTVQAINPLYVSGTVDSWITELIWDRLMRMGQDGLPKPWAAKSVEWLNKTKVKVVLRE
                     GMKWHDGKPVTVEDVKFSFEVPKTGEAPMYRPFVKKIKNIEITGDNTLIFNLKEPWAA
                     FETASLAKINIIPKHKWKPIIEDLKDKPKNAESYQADKPIGSGPFKFSKWEFNEKVIL
                     KANKEHFHAPKVENWVVRFVPNMEATLGMLENGEINFLAAYTGDSTLLKQTVDNDAKL
                     TMNSSVDLGFRFLAPNHRVKPFKDKAFRKATAALTNRQIVAQVVYKGFAVPCDSVVSP
                     ALEFWHNPNIDYPSGGVKQARQILKEAGYKWDEQGRLLYPKN"
     gene            6494..7483
                     /locus_tag="VKN64_06605"
     CDS             6494..7483
                     /locus_tag="VKN64_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59945.1"
                     /translation="MNLKWKYLIKRTGQMILTMWIISTVLFFIFRLMPGNPLVAYINP
                     TFTEKQQEILMEQFGLNKPLWMQYFIYLKNLLQGKLGQSFFFKEPVTNVIAKVLPNTI
                     YLMLTSLIMAYVIGIIGGIFLAWKRGETIETYGLVFTLFTRSAPQFWVGMLLLAVFSF
                     QLDIFPSGGVASAGIVYGSELEKLFSLQFYKHLFLPALTLTIYLLGLPLLLMRSNMLD
                     VMDEAYVQMAKMRGVNKIRVMFKYAARNAFLPVITAMAVGIGYAMGGNVVIEKIFSWP
                     GLGRLLVKAVSTSDYPLAQGAFILIAAIIIFMNFFADIFYNILDPRVNIEDRK"
     gene            7489..8364
                     /locus_tag="VKN64_06610"
     CDS             7489..8364
                     /locus_tag="VKN64_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA59946.1"
                     /translation="MLNVLKNVIKRTYKFLLNDKFALAGALIYIFFIIIAILAPYIAP
                     FNPYKMQTVEGQFMMNKSPGDRFLLGTTDMGRDIFSQLVYGVRPALLVGFTAAFFVVI
                     IGTLVGLLAGYYGGKVDSVLMRLTDVAFGIPFIPFVIVLVGFLGPNIWNIVMAMALLL
                     WRNTARVIRSQVLTVRERSFVEAAKVSGASDFRIIFIYIAPNILPLSFLYGSLAMGWA
                     ILTEASVSFLGFGDPDVISWGYMLNDAYISQALTRGAYHWFVPPGICIMLIVMAGFFI
                     GRGSEEILHPKLKEY"
     gene            8378..9388
                     /locus_tag="VKN64_06615"
     CDS             8378..9388
                     /locus_tag="VKN64_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59947.1"
                     /translation="MSLLEVDNLNINYETNKGIRKAVDGISFNLDESQSLGLVGESGC
                     GKSTAVKGIIGVQPKNAFIPEGKIRYKGENILDYSENKMNDLRWSEISFVPQAAMDSL
                     NPVYTVKNQFLEVLKSKGNMEKKKAVKRAKNLFKKVGLSEGLLNYYPHQFSGGMKQRA
                     IIALALALKPGLIITDEPVTALDVIVQHQILREVKELRKEFNISLIMITHDISVIAKT
                     CEYIAVMYAGKIVEKGPANKILKEPLHPYTMGLENSFPDITKPDKELISIPGVPPDLI
                     NPPSGCRFADRCPFAEDICYSESPELKKIDEDHYSACIHTENLQKLRDQSGEVETWRR
                     AI"
     gene            9370..10353
                     /locus_tag="VKN64_06620"
     CDS             9370..10353
                     /locus_tag="VKN64_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA59948.1"
                     /translation="MEKSNIINVSNLKKHFLVDRSLFGGQKEKYLKAVDGISFKLKAG
                     ESLGLAGESGCGKTTTGKLLLKLHSLTGGSYYFDGKDITEFVDKSKEKAFRKNAQLIF
                     QNPYEALNPRFTVYRSLLEPLIIHGIGKKKDRKDKIVQMLDKVNLTPAETYLDKYPHQ
                     LSGGQLQRVVIARALIVNPLFIVADEPVSMLDVSVRAGVLNLLKKITKEMDLTTIYIS
                     HDLSLIQYMCDNVAIMYLGKIMEYGPARDVIQNPNHPYTKALISAVPTPDPELRKEEI
                     NIKKHVSDPIDLPQGCNFQNRCPISESKCSQKTPELREIKQDHYVYCHQFN"
     gene            10366..11121
                     /locus_tag="VKN64_06625"
     CDS             10366..11121
                     /locus_tag="VKN64_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HMA59949.1"
                     /translation="MRIAYVVPGVMEKSEMNRRENLLQSWAFSNAKVEVVGVSEGPAS
                     IESKYEEYLSIPAASKKIYQLEKEGYDAVILGCAGDPGLDGIREITNDMLVTGPGQSS
                     MQLAVMLGKKFSVLTVEESAVDIFTELAYKAGVKNRLSSVKSLDIPVLDLSDNKEQVI
                     NKLIKKGQTAVKEDRADVLILGCMSLGFLEVAEKIEDQLDVPVINPSKTSLKITEAFV
                     DVGLSHSKQAFMTPPKIKNGEAESIEELYVSSK"
     gene            11242..12447
                     /locus_tag="VKN64_06630"
     CDS             11242..12447
                     /locus_tag="VKN64_06630"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HMA59950.1"
                     /translation="MGLNKNYQGYQSYEYLEADYDYKSFEMADQLNRVEGYKVPITEK
                     EEELTEEIMRDTVIISMHEHPVRFPADIKEVFEYTRQGREFTAYKGLAHSPLDGVFDN
                     LMDGTCAITSKHGWKWRDVIYDLGMRLSDIAHQNFVIKCEDVDDIFGAYENGQLALVP
                     TMEGAAMIENELDRIDILYGLGVRSIGITYSEANTLGSGLKEKRDGGLTSFGRQAVER
                     MNKIGIAIDISHSGDRTSKDVIEASDKPVFISHAGARSLWDSPRLKSDEVLKACAKND
                     GVIGIEAAPHTTLTKNNTKHGIESFMEHFEYIKELVGIDHVSFGPDTLYGDHVGLHHA
                     YAEHLSIKDSQSQDKDFNEVDYVKGLENPTEASINIIRWLVKNDYSKQDIQKVIGGNT
                     IRALREIWY"
BASE COUNT         4091 a         2166 c         2454 g         4038 t
ORIGIN      
        1 gattaagaca gccagcatga cccagggata aaactgattg gtgacaactt ctactgtgct
       61 gacctgaaca gcatcttcaa aaggtgcata catcgaactc atcatggaac ccaccactac
      121 cggtaccata taaggtatca aatagtttaa ggttgtcccg gcacagtcca tcaggttagc
      181 cctacggtaa ccgctgatac tatatttttc gcccatttca ttggcgattt caccggtccc
      241 gatgatagca gccgtattga gtccggtgag cacatttaat gaaatcaata aggaaacgat
      301 tgatagttca gctgttctta cacccctgac aaatttggcc atcatattta ttattatatc
      361 gatcccgccc ccttctttga ggaagttaat ctgggcgaat aagattaata caactattat
      421 agtggggacc gagccctgga taccttctaa aataattccg ccggccccaa atggttccgg
      481 tacattaata atcatattcg gggtaataat tccggttaaa agtccgagta ctaaacctgt
      541 aattataccg taactgagag cttctattaa atggtgctgt cttacacaaa gatagacaat
      601 tacaattggc actaacagaa aaagcaggct gagcggctcg ggtgtaccta catattcgct
      661 aatttgatca gaaaggctgc ccccgcggcc gatcaagctg tataatacaa tggtaattaa
      721 agcggttggt attgaatact tgagcctgct tttaaccaca cctccaatat ccatcccctg
      781 ggttgtggcc gaggcaattg ttgtatcgga aaccggtgat aggttatcac caaaagcacc
      841 gcccgagact attgccccaa ttaatagggc ggggttactg ccgaccagat aaccgaccgg
      901 atagaggata ggacttacaa cgaaaatagt tccgaccgag gtgcccgtcg aaagagctac
      961 gattccggtt agtataaagg ttgctactac aaatttactg ccgaccagat tcatattgac
     1021 aagataagtt gctaaagttt ttactaaccc gctttcatta agaatagtac ctgagattcc
     1081 ggccagcatt acagccgcaa ccaagatcat ggcaacccgg gaactaaacc cgtcaattat
     1141 cacattggcg aacttttctt tatccttaac aaagggcatg ataaagatta agggtaataa
     1201 aaagaccacc cagtattcag gtatattggc attttgtaag gctatgtaga ccagtccaac
     1261 taaggccaat aagatgggga caaagaccat atactttcca ccgtaaaact ttaaagcatc
     1321 attttgtttc gttctttcca tttttaatac tcactcctct catactctct acttaaataa
     1381 tatttctttc cttaagatca attacaatgt cagcaagccc taattccctt aacttgtttt
     1441 cggtcggaag accggttgcg acttcccaac cgcgtgcttt atagtattca tctaacatcg
     1501 gttctaactc tactatactt ccggctgatt caccgcattc ttccggtagt ggttcattta
     1561 aaaaacgatc cggtaggctg tcatcttcgc gcctgattcc ttctctgact atataggccc
     1621 tctccacatt catgattctt tccgctgact tttgtatttc ttcaactgtg aaatcatatt
     1681 cggttaaggc atttaaaagt tcggcagctt ctttaaaaga gataatttcc atatttacta
     1741 ccgtgttttt acaggcttcc agacagtcgg ccaggacgca tctttcttca taaaatttta
     1801 caaccctacc tttgccttta tactccagtc taaaagctga ttccggatgt ccaaaccttc
     1861 ttttaccctc ggcagcattt tgactgaatt caaaccaggg ttcactgcgc aggtggtcac
     1921 ctcctctggt agcaactgct accccgaggc cataaccttt aataccgcgc ggatcagcct
     1981 gaaagatttc taaaccttta acatgcatgg ccaatttttt tgtatcttta ccgattcttt
     2041 cagcagcttt gacaaccccg tctgctaaaa ggttaccaaa gccttctctg ttttttatct
     2101 tttctattaa ggcaaaaacc gcttcactgt ttccgaagtt aggtttgagt ccatcaactt
     2161 cttcactact taagatacct ttttcataaa gttccatggt aaaggcgata cattctgaag
     2221 tagtaattac atctaggccg taacggttac ataaatctac accatgtagg gcatcttcaa
     2281 tatccgagac accaacccgg gaagaaaaac cggctagtcc ttcaaattcg ggcccttcgc
     2341 tttttaatcc tttgtatttg ccctccttaa tttcaaaaaa tctactgcag ggaattgtac
     2401 aggcaaaaca gcccttttcc ttctgcttat agttttcttc taagtattct ccacttactt
     2461 tatcaccagc ttcaaagtag ccggtctgga aatgtcttgt ggccagacag cccagttcat
     2521 ttaaagaaga taccagctgg gtagtaccga gtctggtcct tatctcatat tcagaatggt
     2581 ttttaatttc ttgattcatc ttatctatta atgttttgaa cttgtccggc tttttagctc
     2641 cgagatccct ggtacctctc actgctatcg ccttaatatt tttactggcc agaaccagtc
     2701 ccataccggt tctagctgca gcccgcacca tgttacagaa gataccggca tacttaacac
     2761 cgttttcagc tgccggcccg atggcggcta tttgtacccg actgtcaccc agttcttcct
     2821 ttatcttctc ctgtgtctca aaagtatcta actgttttaa gtgtttggct gatctaattt
     2881 ccgcctttcc atctttgata tatatgtagg aaagctggtc tgctttacct tccagaataa
     2941 tttgatcaaa gccggcaaat ttaatttcag gccctaaaaa gccgcctgta tttgaatccc
     3001 ccagaatatt ggtttggggt gatttggctg taaagtttac ccgggccgct cccgggaata
     3061 gggttccggt taaagggccc acgccaataa acatcttatt atcttcactt aaagggtcaa
     3121 cttcaggggg aacttcctca taaaggcggc gtacattcaa tccgcgtccc ccgagataag
     3181 cttttaaaaa ttggggatcc aatgtttctt tttctattgt attattactt aaattgatac
     3241 ggagcgcttg attacagtaa ccgtaattca ttattctttt ccccctttat tatcaaactt
     3301 taaagcatcg gttggacatc tttttacaca ttcgacttcg cccccacaga gattacagat
     3361 taaggcagta tcatcatccg taaaagtgat tgcatcaaag ggacaggcct ctgcacattt
     3421 aaagcagccg atacatttat catcatcaac taagacagcg ttagttgcag gatctctact
     3481 taaagcatca accggacagg ctttgataca gggggcatct ccacactggc ggcagacata
     3541 ggggatatct tccccggccc gttcattctt ttcaattttc atacgggagt ttttggcatt
     3601 aaaactctga ctatgtacaa aagcacagac cgtttcacag gcccgacagc ctgtacaggc
     3661 ctccggatcg acctggatcc ggggtggtat ttctttttct ttatcaacta ccttccctgt
     3721 tttaggatca attctatcta tcctaaagac aactccacca tttggatctg gacagataaa
     3781 tcttttggta tgtggaatcc agtcattttc ttctttgatt tctctctgct tagccggtag
     3841 aaccggcatt agactctgca ttgcccagag acacatatgt ttaccctcag gaatttctat
     3901 ctggccaccg ctgacggtaa agtagtctcc gacttccatc ggcaggtcac aaaaaccttc
     3961 tatttcttct acaactatct ttagatcata caaattaaac aactcctttc tggttattaa
     4021 aaatttatga aaattaaaaa aggtggcacc acgcaattaa aaaaaccttc ttcccgatag
     4081 ggacgaaaga ttaataattc cgtgttgcca cccttgttag ttaataataa ttaactcact
     4141 ttgttaaaaa ccatcagtca aaagatgatt tttatttact gttaacgggt aaaaaccggc
     4201 tttaggatac tctatcttaa aaagatattt cccccagctt ctcacaaggc cattcacttt
     4261 attatttcat taccgaattc tcagcagtct cggctctctg taaatgaaag tataaagtta
     4321 cttttcttgg tcaatgaaat tatcttattt tattgttttt tattttacca ctataaagta
     4381 acgttgtcaa gtaaattact ctttaaagta tatatataca tcacctatat tttttcaaat
     4441 attagataaa gtttctgttc ttagccagat agtaaaaagc cctatacaaa gaggctttta
     4501 agctataagg ctgaaagcaa actgtatata aaatttagaa aatttataat ttcttatata
     4561 gtttgtaata aatatcgttc tattttataa ttatgtgaaa taaataatag gagaaagcta
     4621 ttataaaaat tctagactgg ttatttataa agaaattttt gtatatatta cttaaattag
     4681 ttgggaggtg aaatataaaa tcacataatt tgagagtagt taagtagaag tttttataag
     4741 tgtttgagaa atttatataa aacgaattat aggaggagat aaaattgcgt aggaaaataa
     4801 gatttatgtt aagttttgtt ttaatcttgg tattaatatt accgatattt tcacaaactg
     4861 tatttggtgc tgaaggtgat agggttgttg atgtgacttt actgaccaga cctaaatcgt
     4921 ctgcttctaa tgagtatgaa gcgatcaact tggtagcaaa gaatttacgc aaaattggtg
     4981 ttcaagctga agttaaagta actccttggg aacagatggc ggatgtagtc tggtatcaaa
     5041 gagaatcctg ggatattact ggttggcaga tgaccgcacg gccagaacgt ttagatccag
     5101 atgaatttat atataacttg tttcattcaa gtactgctga aaaaggttat aattttatag
     5161 gttataaaaa cccgcaatat gataaaattg ctgagaaaca aagggttact atagaccgag
     5221 aaaaacgtaa agagttaatt tataaagctc aaaaaattct tgctgaagat gttgcatata
     5281 tttattctgt tcatcctaaa ataaataatg tatataacaa ccaagtattt gccgatgatt
     5341 ctatgaagga gatggcggga ttaggcctta agaatttttg gacatatata aacgcaaaac
     5401 ctattagtga tcaaaaagat atcgttttaa actttaatga tactgttcag gcgataaatc
     5461 ctctttatgt gagcggtaca gttgattcct ggataacaga attaatctgg gatcggttga
     5521 tgagaatggg tcaagatggt ctaccaaaac catgggcagc caaaagtgtt gaatggctta
     5581 ataaaactaa ggtaaaagtt gtactaagag aaggtatgaa gtggcatgat ggtaagccag
     5641 ttacagtcga agatgttaaa ttttcatttg aagtaccaaa aactggtgaa gcacctatgt
     5701 atagaccgtt tgttaaaaag ataaaaaata tagagataac tggagataac acccttatct
     5761 ttaacctaaa agaaccatgg gcagcttttg aaacagccag tttagctaaa attaatatta
     5821 ttcctaaaca taaatggaaa ccaattattg aagatctcaa ggataaaccc aaaaatgcgg
     5881 aaagttatca ggcggataaa ccgattggct caggtccctt taaatttagt aaatgggagt
     5941 ttaatgaaaa ggtaatttta aaagcaaata aagaacactt tcatgcccct aaagttgaaa
     6001 attgggtggt aagatttgta cctaatatgg aggctacttt aggtatgcta gaaaatggag
     6061 aaataaactt cttggcagcg tatactggag attcaacttt attaaaacaa actgtggata
     6121 atgacgcaaa gttgacaatg aattcttctg tagatttagg atttagattc cttgccccta
     6181 accatagagt taaacccttt aaagataaag catttagaaa agcaactgca gctttaacta
     6241 atagacagat agttgctcag gtagtatata aagggtttgc tgtcccctgt gatagtgttg
     6301 tttcacctgc cttagaattt tggcacaatc caaatataga ttacccatct ggtggagtaa
     6361 aacaggctag acagatatta aaagaagctg gttataaatg ggatgaacag ggaagattat
     6421 tataccctaa aaattagtca actttacact gcagggggtt tattcccctg cttattaaat
     6481 tggagggatt tatttgaatt taaaatggaa atatttaata aaaagaaccg gacagatgat
     6541 tttaactatg tggattatat cgactgtttt attctttata tttcgattga tgcccggtaa
     6601 tcctttagtt gcatatataa atccaacctt cactgaaaaa caacaggaaa tattaatgga
     6661 acaatttgga cttaataagc ctctatggat gcagtatttt atttatctaa agaatttatt
     6721 acaaggtaag ttaggtcagt ctttcttttt taaagaacct gttacaaatg taattgctaa
     6781 ggttttgcca aatacaatat atttgatgtt aacttcatta attatggcgt atgttatagg
     6841 aattatcggg gggatttttc ttgcttggaa aagaggcgaa acaattgaaa cttatggatt
     6901 agtatttacc ctatttacaa ggtcagctcc ccaattttgg gtaggaatgc ttttattggc
     6961 tgtcttctct tttcagttag atatttttcc ttccggtggg gtggcttcag ccggaatagt
     7021 gtatggttcg gaacttgaaa aattattttc actacagttt tataagcatc tgtttttgcc
     7081 ggctttgacc ttaactatat atctgttagg attacccctc ttattaatgc gttctaatat
     7141 gcttgatgta atggatgaag cttatgtaca gatggctaaa atgagaggag taaataaaat
     7201 tagagttatg tttaaatatg ctgctcgcaa cgcttttttg ccagtaataa ctgctatggc
     7261 tgtaggcatc ggatatgcta tgggcggaaa tgttgtaatt gaaaagatat tcagctggcc
     7321 tggtctaggt agattattgg taaaagcagt ttctactagt gattatcctc tagctcaagg
     7381 agcctttata ttgatagctg ctataataat atttatgaat ttttttgccg atattttcta
     7441 taatatatta gatcccagag taaacataga agataggaag tgatagaaat gctaaatgtt
     7501 ttaaaaaatg taattaaaag aacatataaa tttctattga atgataaatt tgcattggca
     7561 ggagctctaa tctatatatt ctttattata attgcaattt tagctcccta tattgctcct
     7621 tttaacccct ataaaatgca gacagtagaa ggtcagttta tgatgaacaa atcacctggt
     7681 gatagatttt tgctagggac tacagatatg ggtagagata ttttttccca actggtatat
     7741 ggagtaagac ctgctctttt agtgggtttt acagctgcct tttttgtggt aataatcggt
     7801 actttagttg ggctattggc aggttattat ggagggaaag ttgacagtgt tttaatgcgt
     7861 ttgacagatg tagcatttgg tattcctttt ataccatttg tgattgtttt agttggattt
     7921 ttgggaccaa atatctggaa tatagtgatg gcaatggcgc tgctactttg gagaaacaca
     7981 gcgcgtgtaa ttagatccca ggttttaaca gtaagagaaa gaagctttgt ggaagcggct
     8041 aaagtgtctg gagcatcgga ttttaggatc atttttatct atattgctcc taatatctta
     8101 cctttatcat ttctatatgg ctcgcttgca atggggtggg ccattttaac agaagcttct
     8161 gtgagttttt taggttttgg agatccagat gttatcagct ggggctatat gttaaatgat
     8221 gcctatattt cccaggctct tactcgaggt gcgtatcact ggtttgtacc tcccggaatc
     8281 tgtattatgt taattgtaat ggctggattt tttataggca gaggctcgga agaaatttta
     8341 catccaaaat tgaaagaata ttaaaaggag ggaacaaatg tcattactag aagttgataa
     8401 tttaaatata aattatgaaa ctaataaagg tatccgaaag gcagtagatg gaatttcttt
     8461 taatttagat gaaagccaga gtcttgggtt agtgggagaa tctggttgtg gcaagtctac
     8521 tgctgttaaa ggaataatag gtgtacaacc aaagaatgct tttataccag aaggtaaaat
     8581 aaggtataag ggggagaaca ttttagatta ctcagaaaat aaaatgaatg atttaagatg
     8641 gtcagagatt tcttttgttc cccaggctgc catggattca ttaaacccgg tttatactgt
     8701 aaaaaaccag tttctcgaag tattaaaatc aaaaggtaat atggaaaaaa agaaagcggt
     8761 aaaaagggct aagaatttat ttaaaaaagt tggtcttagt gaaggattgt tgaattatta
     8821 tcctcatcaa ttttcaggtg gaatgaagca gagagctata attgctcttg ctttagcatt
     8881 aaaacccgga ttgattataa cagatgaacc agtaacagct cttgatgtaa ttgttcaaca
     8941 tcaaatatta agagaagtca aagaacttcg taaggaattt aatatttctt tgattatgat
     9001 tacccatgat atttctgtaa ttgcaaaaac ctgtgaatat atagcggtaa tgtatgctgg
     9061 taaaatagta gagaagggtc cagctaacaa aatactgaaa gaacccctgc acccttatac
     9121 catgggattg gaaaattctt ttcccgatat aacaaaacca gataaagaat taatttctat
     9181 acctggggta ccaccagatt taattaaccc cccttcgggc tgtagatttg cggatagatg
     9241 tccatttgca gaagatattt gttatagtga atcaccagaa ttaaagaaaa tagatgaaga
     9301 tcactattct gcctgtattc atacagaaaa cttgcaaaaa ctacgggatc aatctgggga
     9361 ggtagagaca tggagaagag caatataatt aatgttagta atttaaaaaa gcatttttta
     9421 gttgatagaa gtttatttgg cggtcaaaaa gaaaaatatt taaaagcagt agatggtatt
     9481 agtttcaagt taaaagcagg agaatctcta ggtttagccg gagaatctgg ttgtggaaaa
     9541 actactactg gtaaactttt attaaagctt cattctctga caggaggttc atattatttt
     9601 gatggaaaag atattactga attcgttgat aaaagtaaag aaaaagcttt tcgcaaaaac
     9661 gctcaactga tatttcaaaa tccttatgag gctttaaacc ctcgttttac ggtttatcga
     9721 tcattattgg aaccacttat aatacatggt atcggcaaga aaaaagatag aaaagataag
     9781 attgttcaga tgctagataa agttaatttg actcctgctg aaacgtattt agacaagtat
     9841 cctcatcaat taagcggagg ccaacttcag cgggtggtga tagccagagc tttaatagtt
     9901 aatcccttat ttattgtggc tgatgaacca gtgtctatgt tggatgtttc agtgagagct
     9961 ggtgttttaa atcttttaaa aaagatcacc aaggaaatgg acttaacaac tatctatatt
    10021 tcgcatgatc tttctttgat tcaatatatg tgtgataatg tagccataat gtatttggga
    10081 aaaattatgg aatatggacc agcccgagat gtgatacaaa atcctaatca tccctatact
    10141 aaagctttaa taagtgcggt gccgacccca gaccctgagt taagaaaaga agaaattaat
    10201 attaaaaaac atgtatctga tccgattgat ttacctcaag ggtgtaattt tcaaaataga
    10261 tgtccaatat ctgaaagtaa atgtagtcaa aaaacccctg agttaagaga gattaagcaa
    10321 gatcactatg tctattgtca tcaatttaat taaggagtga gagatatgag aattgcatat
    10381 gtagttccag gtgtaatgga aaaaagcgaa atgaatagaa gagagaattt attacaaagt
    10441 tgggctttct ccaatgcaaa agtagaagta gtaggtgtaa gtgagggtcc tgcttctatt
    10501 gaatcaaagt atgaagaata tttaagtatt ccggctgcct ccaaaaaaat ataccaatta
    10561 gaaaaagaag gttatgatgc agttattctg ggctgtgcgg gcgatccggg cctggatggg
    10621 attagagaaa ttactaatga tatgctggtt actggtcccg ggcaatccag tatgcagctg
    10681 gctgttatgc tgggcaaaaa gttttctgtt ttgacagtag aggaaagcgc tgttgatatt
    10741 tttacagagt tagcctataa ggccggggta aaaaatcgcc tctcttctgt taaatcattg
    10801 gatatacctg tattggattt atcggacaat aaagaacagg taataaataa gctgattaaa
    10861 aaaggccaaa cagccgtcaa agaagatcgg gcggatgtac ttattttagg ttgtatgtct
    10921 ctcggttttt tagaagtggc tgaaaaaata gaagatcaac ttgatgtacc agtaataaac
    10981 ccctccaaaa ctagtctaaa gattacggaa gcttttgttg atgtgggttt aagtcattct
    11041 aagcaagctt ttatgactcc acctaagata aaaaatggag aggcagaaag tatagaagaa
    11101 ctttatgtca gcagcaaata aggttatatt atttataaat taaatttttt aaaacagatc
    11161 attttaaata attataaaat agtgatcaat tatctttgat ttaattatat ataaatacct
    11221 aaaaattaag gagatgatta aatgggttta aataaaaact atcaaggtta tcaatcatat
    11281 gaatatttag aagctgatta tgattataaa tcatttgaaa tggcggatca gttaaataga
    11341 gtggaaggtt ataaggtccc tataactgaa aaagaagaag aattaacaga agagattatg
    11401 agggataccg tcataatttc catgcatgaa caccctgtcc ggtttccagc cgatattaaa
    11461 gaagtatttg aatatacaag gcagggtaga gaatttactg cgtataaagg tttagcacat
    11521 tcacccttag atggtgtttt tgataattta atggatggca cttgtgctat cacatcaaaa
    11581 cacggatgga aatggagaga tgtaatatat gatttaggga tgcgtttaag tgatattgca
    11641 catcaaaatt ttgtgatcaa atgcgaagat gtagatgata tatttggtgc ttatgaaaat
    11701 ggtcaattag cgctagttcc tacaatggaa ggagctgcta tgattgaaaa tgaattggat
    11761 agaattgata ttctttatgg gttgggagta cgttctatag gtattacata tagtgaagct
    11821 aataccttag gttcaggttt gaaagaaaaa cgagatggcg gtcttacttc ttttggtaga
    11881 caagctgtag agaggatgaa taaaattggt atagcaattg atatttctca ctcgggcgat
    11941 cgcacctcaa aagacgtaat cgaagcaagt gacaaaccgg tgtttatctc acacgctggt
    12001 gccagatctc tttgggatag tccgcgactt aaaagtgatg aagttttaaa agcctgcgct
    12061 aaaaatgatg gtgttatagg aattgaagct gcacctcata caactttgac taaaaataat
    12121 acaaaacatg gtattgaatc atttatggag cattttgaat atattaagga actagttgga
    12181 attgatcatg taagctttgg tccggatacc ttatatggag atcatgtggg attacaccat
    12241 gcttatgcag aacatctgtc cataaaagat tcccaatccc aagataagga ttttaacgag
    12301 gtggattatg taaaaggttt agaaaatccg actgaagcct ccataaatat tattcgctgg
    12361 ttagttaaaa atgattacag taaacaagat attcaaaaag taatcggtgg taatactatc
    12421 agagctctta gagaaatatg gtattaaaag aaatactatt tattaataga atattaatta
    12481 tattataaaa agtaggtaga agtctgtcag ttaaatttaa catcttataa ctgacagact
    12541 tttttgttaa ttaatcagta aaaatatagg atatttcatg ggttttttat gatatttaaa
    12601 atagctttaa tttttaaatt cagattcgac ttgagaagca aaaatatgtt atactagcag
    12661 tggttattcg ttgagccttt tattgttata gaaaaatttt taatgttcta ttccatttaa
    12721 gaaataactt tttatttaaa ttttcaacc
//
LOCUS       DATGCJ010000131         6733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_107308, whole genome shotgun sequence.
ACCESSION   DATGCJ010000131 DATGCJ010000000
VERSION     DATGCJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107308"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1248
                     /gene="dapG"
                     /locus_tag="VKN64_06635"
     CDS             37..1248
                     /gene="dapG"
                     /locus_tag="VKN64_06635"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMA59951.1"
                     /translation="MSIVVLKFGGTSLKNNKASAAAVKRIDEIIRKGKRPVVIVSAIG
                     REGSAYATDTLIQFAEDTFPELAKREKDLIMSCGEIISSVVMVQNIKKKERQARALTG
                     AQAGILTDENYCNSDVININPENILKLINNDIIPVIAGFQGISENGEITTLGRGGSDT
                     TASIIAAGLAAEKIEIYTDVEGLLTGDPSIIEEAKFLPKANYTEACELAYQGAKVIHP
                     AAAEIAMKNKIPVEIKSTFNNKEGTLIDKLGDEKKLTPITGLAARDEIFYVKISAHQG
                     INYDNGLQVFQLLADQSISVDFINIRESVISFVIDNEKRELSKEILSKNDFDFEINEE
                     YVKISIVGGGMTGQPGIMARIVNTLHDVGVKIYQTTDSHTVISCLIKEKDKIKALNAL
                     HDAFNLNTIGG"
     gene            1253..2140
                     /gene="dapA"
                     /locus_tag="VKN64_06640"
     CDS             1253..2140
                     /gene="dapA"
                     /locus_tag="VKN64_06640"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMA59952.1"
                     /translation="MHKSFGNLLTAMVTPFDNNLKVDYSKVRKLSRHLVDEGCDGLVV
                     LGTTGEVPTLNFQEKLEILRTVVSEVGEDANVVAGTGSYSTEASKKLTKEAEEIGVDG
                     VMLVVPYYNKPPQYALYNHFKEIAESTELPVMIYNVPSRTSRNIEPETVKKLVQDVDN
                     IIAIKEASGDLGQVAELSLIERDDFYIYSGDDMLTLPIMSLGGEGIVSVAGHVAAKKI
                     KEMITLYKTGNPVQSRELNKKLIPVYNSMFIDTNPIPVKEALKITGFDAGSPRPPLYK
                     LTEEKREKLAKVLKEADLI"
     gene            2216..3904
                     /locus_tag="VKN64_06645"
     CDS             2216..3904
                     /locus_tag="VKN64_06645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HMA59953.1"
                     /translation="MEVNNLANDTHSDITITPLGGVGEIGKNMMLFEMDEEMIIIDSG
                     VKFPEDDLLGIDLVIPDFTYVMENKDKVKGIVLTHGHLDHIGALPYLLKELDVPVYGT
                     KLTLGLLQGNLKEHNLQGKRKLKVVTPGKSYQVGSREVEFIRVNHSIADTCALAIHTP
                     EGPIVYASDFKFDQTPIDGEVADFHKLAELGDSKPGVLALFSDSTNAERKGYTLSEKV
                     VGGTIDDIFRETRDRIVVATFASNIHRVQQIADAAMKFNRKIAFTGRSMIENVKTARE
                     LGYLTIPEDVILDIRKISSLPNDQVVLLTTGSQGEPMAALTRMARGDHYHINIKQDDT
                     VIISANAIPGNEKFVGQTINQLFERGANVIYEDISGVHVSGHASQEELKLMMNLVKPK
                     YFVPTHGEYRHLHKHAELAEKVGIPKDNIYIAEIGDVIKFGPEKVEKKNNITAGDVLV
                     DGLGVGDVGNIVLRDRKMLSEHGILIVVVTIDKNGNIIAGPDIITRGFVYIRESEKLI
                     SDAQERVREALRECEENNITEWSKWKSTIKDSLNNYIYSRIHRKPMIMPIIMEI"
     gene            3970..4749
                     /locus_tag="VKN64_06650"
     CDS             3970..4749
                     /locus_tag="VKN64_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMA59954.1"
                     /translation="MEIVKIVILGIIQGLTEFLPISSSGHLVIMQKLFNIEQNQLLIS
                     VLLHFGTLIPVLIVFWEDIKEILYFKKEKRYFIFLIIIASIPTALMGVFFEDFFEKLF
                     SSTLSTASMLIVTGFILYLGDNLADYEKEITNFKWLNAVLVGIAQGIAIIPGISRSGS
                     TITASLIQGLKKEEAARFSFILSIPVIGGAGLLKVGEAVNTGVTNFNMTAIILGIISS
                     IISGYIAIRYFIYILKKQKLIYFSYYCWSIGTIILLYEGLF"
     gene            4784..>6733
                     /locus_tag="VKN64_06655"
     CDS             4784..>6733
                     /locus_tag="VKN64_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HMA59955.1"
                     /translation="MSKQKKKGDIKIEKRKNEILGIMLTAFSIFFFLTIYFGYNSMLG
                     DRMVEIFRLLIGEGIYILPVMLLLWGILLIRNKTLKFNLKIFGFILLFLTIISFVHLY
                     NHSQLTNGYIFLRNGGGVVGGAITWFLIKGLGIIGSYIILSALLLIGFLLWTDILLHS
                     FFRNLKLSIVKKLTSIKESIVEFSDRIFSQPEEELSYPNSGSSSKNTSNNSTTTRKKK
                     EKKKKKTKPVKNTKQTQKQENENLQEFEIKDKTAKTSKNKSTDGFKLPKLNLLENSNK
                     NKIRLENKSELLEETLASFGVEAEVKEVSHGPTITRYEVQPATGVKVSKIVNLADDIS
                     LALAAPDVRIEAPIPGKSAVGIEVPHQNNLIVRLGDIISTDKFQSADSKLTLALGRGI
                     EGDPIVTDLSKMPHLLIAGATGSGKSVCMGSIITSLLYKASPQEVKLILIDPKKVELI
                     NFEGLPHLFSPVVTDPQKASNVLKYVVQEMDKRYELFSDTRTNSIETYNAKVEKEEDK
                     LPYIVVIIDELSDLMMVAANEVEDNICRLAQMSRAAGIHLVVATQRPSVDVITGLIKA
                     NIPSRISFAVSSQTDSRTILDMGGAEKLLGNGDMLFYPVDYKKPVRIQGTLITNDEII
                     DIVEFLNDQKEEDKTDFEVDLDELSE"
BASE COUNT         2370 a         1005 c         1369 g         1989 t
ORIGIN      
        1 tgttataaaa aacaatataa ttgatggagg agaataatgt caatagttgt attaaagttc
       61 ggtggtacat ctttaaaaaa taataaagcg agtgcagctg cagttaaaag aatagatgaa
      121 ataattagaa aagggaaaag accagttgtt atagtgtctg caattggtag agaaggatca
      181 gcttatgcta ctgatacttt aattcaattt gcagaagata cgtttccaga acttgctaaa
      241 agagaaaaag atttaatcat gtcctgtggt gagattatct cttcagttgt tatggtccaa
      301 aacattaaga aaaaagaaag acaggcccgt gctttaaccg gagcacaagc cggtattttg
      361 accgatgaaa attattgcaa ttccgatgtc ataaatatta atcctgaaaa tattttaaaa
      421 ttaattaaca atgatattat tccagtgatt gccggttttc agggaattag tgaaaatgga
      481 gaaattacta ccttgggtag aggtggtagt gatacgacag cctctattat tgcggctggt
      541 ctggcagctg agaagatcga gatttacact gatgtggaag gtttattaac cggagaccct
      601 tctattatag aggaagcaaa gtttttacca aaggcaaatt ataccgaagc ctgtgaacta
      661 gcctatcaag gggctaaggt aattcatccc gccgctgccg agatagctat gaagaataag
      721 attccggtgg aaattaagtc tacctttaat aataaagaag gtactcttat tgataaattg
      781 ggagatgaaa agaagttaac accgatcacc ggtttagcgg cccgtgatga gatattctat
      841 gtaaaaatat cagctcacca gggaattaat tatgataatg gtttacaagt ttttcaatta
      901 ctggccgatc agagtataag tgttgacttt attaatataa gagagtcggt tatttcattt
      961 gttatagaca atgagaagag agaattatca aaagaaatat tatctaaaaa tgactttgat
     1021 tttgaaatta acgaagaata tgttaaaatt tctatagttg ggggaggaat gaccggacaa
     1081 cctggtataa tggccaggat tgtcaatacc ctgcatgatg ttggggttaa gatctatcaa
     1141 accactgact cccatactgt aatttcctgt ttgataaaag aaaaggataa aataaaggct
     1201 ctgaacgccc ttcatgatgc ctttaattta aatacaattg gaggttaaaa aaatgcataa
     1261 aagctttggt aatttattaa ctgccatggt aacacccttt gataataatt tgaaagttga
     1321 ctattccaaa gtaaggaagt tatcaagaca tctggttgat gaaggttgtg acggtctggt
     1381 agtattaggt actaccggtg aagttcccac cttaaatttt caagaaaagt tagaaattct
     1441 cagaacggtt gtcagtgaag ttggtgaaga tgctaatgta gtggctggta caggttccta
     1501 ttccacagaa gccagtaaga aattaaccaa agaagcagaa gaaatcggtg ttgatggagt
     1561 aatgcttgtc gtaccatatt ataacaagcc gccccaatat gctttatata accattttaa
     1621 agagattgct gaaagtaccg aacttcctgt tatgatctat aatgtaccat ccagaacatc
     1681 aagaaatatt gaaccagaaa ctgttaagaa attggtacaa gatgtggaca atataattgc
     1741 aattaaagaa gcaagtggtg acttagggca ggttgccgaa ttaagtttga ttgaaagaga
     1801 cgatttttat atttatagtg gagacgatat gctgacttta cccattatgt ctttgggggg
     1861 agaaggtatt gtcagtgttg ctggacatgt ggctgccaaa aagataaaag aaatgattac
     1921 cttatataaa actggtaacc cagttcagtc cagagagttg aataaaaagt tgattccagt
     1981 atataattcg atgtttatcg atacaaaccc aattccggtt aaagaagcct taaagattac
     2041 aggttttgat gctggatcac caagaccccc attatataaa ttaactgaag aaaaacgaga
     2101 aaaattagcc aaagtactaa aagaagctga tttaatttaa ataaaaaata tgttaatagt
     2161 tgaaaaataa tatagcataa aacagctcct taaatacagt acaaatatac taattatgga
     2221 ggtgaataat ttggctaacg atacacattc cgatattacc attacaccct taggtggtgt
     2281 tggtgaaatt ggaaagaaca tgatgttatt tgaaatggac gaagaaatga ttatcatcga
     2341 ttcaggagtt aagtttcctg aagatgattt attaggtata gatttggtaa ttccagattt
     2401 tacttatgtg atggaaaata aagataaggt taagggaatt gttttaacac acggacattt
     2461 agatcatata ggggctttac cctatttatt aaaagaactt gacgtacctg tttatggaac
     2521 taaacttact ttaggattgc tacagggtaa tttaaaggaa cacaacttac agggaaagag
     2581 aaaactgaaa gttgtaactc ccggtaaatc ctaccaggta ggatcacgcg aggtagaatt
     2641 tatcagagta aaccacagta tagctgatac ctgtgcttta gcaattcata ccccagaagg
     2701 accgattgtt tatgccagcg actttaagtt tgaccaaacc cctattgatg gagaggtggc
     2761 agactttcat aaactggctg aactgggaga ttccaaaccg ggcgttttag ccctcttttc
     2821 agatagtaca aatgccgaaa gaaagggcta tactttatct gaaaaggtgg tcggtggaac
     2881 aattgatgac attttccgcg agacacgcga cagaatagta gtagcaacct ttgcttctaa
     2941 tattcatagg gtccagcaga ttgctgatgc cgcaatgaaa ttcaacagaa agattgcctt
     3001 taccgggcga agtatgattg aaaatgttaa aactgcccgc gaactgggct atttaacaat
     3061 accggaagat gtaattcttg atattagaaa gataagtagt ctaccaaatg atcaggtagt
     3121 actgttaact accggtagtc agggtgaacc gatggctgct ctaaccagaa tggccagagg
     3181 agaccattac catattaata tcaaacagga tgacaccgtt attatctcag ctaatgcgat
     3241 ccctggtaat gaaaaatttg tgggtcaaac cataaatcaa ctgtttgaac ggggcgcaaa
     3301 tgttatctat gaagacatct ctggagtaca tgtctctgga cacgccagtc aggaagagtt
     3361 aaagctaatg atgaacctgg taaaaccaaa atactttgta ccaacacacg gtgagtacag
     3421 acatttacac aaacacgctg aactggccga aaaagttggt ataccaaagg ataatatcta
     3481 tattgccgaa ataggggatg taattaagtt cggccccgaa aaggttgaaa agaaaaataa
     3541 tattacagct ggagatgtct tagtcgacgg tctcggtgtt ggtgatgtag gtaatatagt
     3601 cttaagagat agaaagatgt tatctgaaca tggtattcta attgtggtag taactatcga
     3661 taaaaatggt aatattatag caggacccga tattattaca agaggttttg tctatattcg
     3721 tgaatcagaa aaattaattt ctgatgccca ggaaagggtc agagaagcat tgagagaatg
     3781 tgaagaaaac aatattaccg aatggtcaaa gtggaaatcc acgataaagg attctctaaa
     3841 taactatata tacagtagaa ttcataggaa gcccatgata atgcccataa taatggaaat
     3901 ataaataagc aaacttaagg gatgggtttt tactcatccc tttcaattac atatggtgga
     3961 ggatatttta tggagatagt aaagatagta atattaggta taattcaggg tttaactgag
     4021 tttttaccga tcagtagttc aggacattta gttatcatgc agaaattatt taatattgaa
     4081 caaaaccagc tcttgatatc agtccttcta cattttggga ccttaattcc ggttctaata
     4141 gttttttggg aagatataaa agagatctta tactttaaaa aagaaaaaag atacttcata
     4201 tttttaatca ttattgcttc tattcctacc gctttaatgg gcgtattttt tgaagacttc
     4261 ttcgaaaaac tcttttcatc tacgctaagt acagcttcca tgttaattgt caccgggttc
     4321 atactctatc ttggcgataa tctggcagat tacgagaaag aaattactaa ctttaagtgg
     4381 ttaaatgctg tattagtagg tattgcccaa gggatagcaa ttatacccgg tatatctaga
     4441 tcaggtagta caatcacagc ttctttaata caaggtttaa agaaagaaga ggcagcccgt
     4501 ttttccttta tattatcgat acctgtaatc gggggtgccg gtttattaaa agtaggagag
     4561 gcggtcaata caggtgtgac taacttcaat atgactgcca ttattttagg tataattagt
     4621 tctattatct ccggatacat agcaatcaga tattttatct atattttaaa aaagcagaag
     4681 ttaatctatt tctcatatta ctgctggtcc attggaacaa tcattttatt gtatgaagga
     4741 ttattttaaa tgttattgaa tatatatagt tgaggtgaaa gttttgtcaa agcagaaaaa
     4801 aaaaggagat attaaaatag aaaaaagaaa aaatgagatt ttaggaatca tgttaaccgc
     4861 ctttagtata ttcttttttc taaccatcta tttcggttat aacagcatgt taggggatcg
     4921 gatggttgaa atatttcgtt tattaattgg agaaggaata tacatcctac cggtcatgct
     4981 actattatgg ggaatattac tgattagaaa taagaccctt aaatttaatt taaagatttt
     5041 tggttttata ttactatttt taacaataat ttcttttgtg catttatata atcattccca
     5101 actgacaaac ggttatatat ttttaagaaa tggtggggga gttgtaggtg gcgctataac
     5161 ctggttttta attaaaggat taggaataat aggttcctat ataatccttt cagccttatt
     5221 actgatagga tttttattat ggacagacat attattacat tcatttttca gaaatttgaa
     5281 attaagtatt gttaaaaaac taacttctat taaggaaagt attgtagaat tttcagatcg
     5341 catatttagc caaccggaag aagagttaag ttatccaaat agtggttctt ccagtaaaaa
     5401 cacatctaat aattctacaa ctactcgcaa gaaaaaggaa aagaaaaaga aaaagactaa
     5461 accagttaaa aatacaaaac agacccaaaa acaagaaaat gaaaacttgc aggaatttga
     5521 gataaaagat aaaactgcta aaacaagtaa aaataaatct accgatggat ttaaattacc
     5581 taagttaaat ctgttagaga attcaaataa aaataagata agattagaaa acaaaagcga
     5641 actattagaa gaaacattag ccagctttgg ggtagaggca gaggttaaag aagtaagcca
     5701 cggaccaact attaccaggt atgaagttca gcctgccact ggtgttaagg tgagcaagat
     5761 agtaaactta gctgatgata tatctctggc cctggcggct ccggatgtga ggattgaagc
     5821 ccccattccc ggtaagtcgg ctgtaggtat cgaggtacct catcagaata atttaattgt
     5881 aaggttagga gatataattt cgaccgataa atttcaatca gctgacagta agttgaccct
     5941 ggctttaggc aggggaatag aaggagatcc gatcgtaaca gatctttcca aaatgcccca
     6001 ccttttaata gcgggtgcaa caggctctgg taaaagtgtc tgtatgggaa gtattattac
     6061 cagcctatta tacaaagctt caccgcagga agtaaagttg atcttaattg accccaaaaa
     6121 agtagagttg attaatttcg aaggtttacc tcatcttttt tcacctgtgg ttactgaccc
     6181 acagaaagcc tctaatgtat tgaagtatgt agtacaggaa atggataagc gatatgaact
     6241 attttctgat accaggacaa acagtattga aacttataat gcaaaggtgg aaaaagaaga
     6301 ggataaactg ccatatatag tggttattat agatgaattg tctgatttaa tgatggttgc
     6361 tgccaatgaa gttgaggata atatctgtcg tctggcccag atgtccaggg ccgcaggtat
     6421 acatctggtt gtagctactc agagaccatc tgttgatgtt atcacaggtt taattaaagc
     6481 aaacattccg agcaggattt cttttgccgt atcatcacag acagattcca gaacaatatt
     6541 agatatgggg ggtgctgaaa aattacttgg taatggagac atgctcttct atccagtaga
     6601 ttacaaaaag cctgtgagga tacagggaac attgattacc aatgacgaaa taattgatat
     6661 tgtagaattt ttaaacgatc aaaaagaaga agataaaaca gattttgaag ttgatttaga
     6721 tgaactaagt gag
//
LOCUS       DATGCJ010000132         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_107317, whole genome shotgun sequence.
ACCESSION   DATGCJ010000132 DATGCJ010000000
VERSION     DATGCJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107317"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="VKN64_06660"
     CDS             <1..2032
                     /locus_tag="VKN64_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HMA59956.1"
                     /translation="KKILVKFDGIMKKAEVFVNGTLVGTHKGGYTSFKLDITDYVNIN
                     ENNLIALKVDSRCKDNIPPEGNKVDYLLFGGIYRRVQLIVTGNSYIDWTFYKIKECTP
                     KKSVIEPKINIINSQNKGKYNIKVQLEDDDQVIKEKEYTVDLKENENELKLDDFSINN
                     PELWYPDNPKLYRVRTQIKDSQDNIIDETTERIGLRKVEFKADGLFYINDQKLKLRGL
                     NRHQLYPYVGNAMPDKGQIKDADFLKEELGLNFVRSSHYPADPAFLDRCDEIGLLVFE
                     EIPGWQHIGDEDWQEIAYQNLEEMIIRDRNHPSIFIWGVRVNESGDNHQFYTRTNQMA
                     HQLDSTRPTGGVRNFRDSEFLEDVFTYNDFAGNLEGEIKEPNYKPYMICEYMGHMYPT
                     KSYDSVERQIKHAVKHLQIQDKQYQNDDIAGASGWCAFDYNTHADFGSGDRVCYHGVC
                     DMFRTKKMAAYVYQSQLDPKQKPVIHIARYLTPSFNEDYGDKIIVFSNCEQVKLFVND
                     KLEKTARPAVDKYPGLPHPPFVFEDCRWWEWGASTIESLKAVGYIDNKEVCEHTIYPF
                     TEPEKIKLKADNEVLKADGADLTRLEIRLVDKNEQTLLWAHNPVFFKVEGPGKIIGET
                     PFSLEAGRGAVYLQSTREPGLIKIKAKSPNYESEVIEIKSRKLKEKIVPIKK"
     gene            2210..>3045
                     /locus_tag="VKN64_06665"
     CDS             2210..>3045
                     /locus_tag="VKN64_06665"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HMA59957.1"
                     /translation="MTRPIDDINRVILIILDSVGIGALPDAEEYGDQGANTLVHTAEA
                     VGGLDLPNMERLGLGKIAAVKGIKSNIKAEGSYTKMKEKSVGKDTTTGHWELAGLVSE
                     KPFPVYPNGFPEEVIKTFEKKIGKEVLGNKPASGTVIIEELGEEHLNTGKPIVYTSAD
                     SVFQIAAHEDIISVEELYEMCEVAREILQGEHGVARVIARPFVGELGSFTRTDRRKDF
                     SLAPPRATVLDKLKARGISVRAVGKIEDIFSNKGITDSNHTVDNMDSVDAVIEYLKED
                     KKS"
BASE COUNT         1054 a          493 c          669 g          829 t
ORIGIN      
        1 caaaaagatc ttagttaagt tcgatgggat aatgaaaaag gcggaagttt ttgttaatgg
       61 aactttagtc ggtacacata aaggtgggta tacttccttt aaattagata ttactgatta
      121 tgttaatatt aacgagaata acttaatcgc cctgaaagtt gattcccgct gtaaagataa
      181 tataccaccg gaaggcaata aggtggacta ccttttgttt ggtgggatat ataggcgagt
      241 ccagctgata gtgaccggaa atagttacat agactggact ttttataaaa ttaaagaatg
      301 taccccgaaa aaatcagtta tcgaaccaaa gatcaatatt ataaattctc aaaataaagg
      361 taaatataat attaaagttc agttggaaga tgatgatcaa gttattaaag aaaaagaata
      421 tacagttgac ttaaaagaaa acgaaaacga actaaaacta gatgatttct caatcaataa
      481 ccccgaactt tggtatcccg ataatcccaa actatataga gttagaaccc aaataaaaga
      541 tagtcaggat aatataatag atgaaacaac agagcgaata ggtttaagaa aagtagagtt
      601 caaagccgat ggactctttt atataaacga tcaaaaatta aaattaagag gtttaaatag
      661 acatcaactt tatccctatg tcggtaatgc gatgcctgat aaaggtcaga ttaaagatgc
      721 ggactttcta aaagaggaac ttggtttaaa ctttgtccgt tcttcccatt atccggccga
      781 tcccgccttt ttagaccgct gtgatgaaat cgggctttta gtatttgaag aaataccggg
      841 ttggcagcat atcggagatg aagactggca ggaaatcgcc tatcaaaact tagaagagat
      901 gattatcaga gataggaacc acccttctat ctttatctgg ggagttaggg tcaatgagtc
      961 cggtgataac catcaattct atacaaggac caaccagatg gcccatcagc ttgattccac
     1021 ccgcccgacc gggggagtcc gtaatttcag ggacagtgaa tttttagaag atgtctttac
     1081 ctataatgac tttgcaggta atttagaagg tgaaataaaa gaacctaact ataaaccata
     1141 tatgatatgt gagtatatgg gacatatgta tcccactaag tcctatgata gtgtagaacg
     1201 acagattaaa catgctgtta agcacctgca gattcaggat aaacagtatc aaaatgatga
     1261 tattgctggg gccagcggct ggtgtgcctt tgactataat acccacgctg attttggttc
     1321 gggggatcgg gtttgttacc acggggtctg tgatatgttt agaactaaaa aaatggcggc
     1381 ctatgtttat caaagccagc tggatcccaa acaaaaacct gttattcata tagcaagata
     1441 tttaaccccc agttttaatg aggactatgg ggataaaatc atagtattta gtaactgtga
     1501 gcaagtgaag ctatttgtta atgataagtt agaaaaaaca gctcgaccag ctgtcgataa
     1561 gtatccgggt ttacctcatc caccctttgt gtttgaagac tgccgctggt gggaatgggg
     1621 agccagtacg atcgaaagcc ttaaagcagt tggatatatt gataacaaag aggtttgtga
     1681 acataccatc taccccttta cagaacccga aaagattaag ctaaaagcgg ataatgaggt
     1741 tttaaaggcg gatggagctg atttaacgag attagagatc cgccttgttg ataaaaatga
     1801 acagaccttg ctctgggccc ataaccccgt cttttttaag gtagaaggcc cgggtaagat
     1861 tataggggaa acaccattta gtttagaagc ggggcggggt gctgtttatc tgcagagcac
     1921 ccgtgaacct ggtcttatta aaataaaggc taagtcccca aactatgaaa gtgaagtaat
     1981 tgagattaaa agtagaaagt taaaagaaaa aattgtaccg ataaaaaaat aataaaattt
     2041 cctcctccat tcaccttaaa aaagggaatg gagttttttt atttaatttt gaataagggg
     2101 caaacaaacg tattcttaat atgttgctat ttaacccaat atattataaa attataaggt
     2161 aaagtaaagg tattatagta aaatattaac aatcattgga gttgatataa tgacaagacc
     2221 aattgatgat ataaacagag tcatattaat aattttagat agtgtcggga ttggtgccct
     2281 gccggatgct gaagaatatg gtgatcaggg agccaatacc ctggttcata ccgcagaagc
     2341 ggtcggtggc ttagacctac ctaatatgga aagattgggt ttaggtaaga tcgcagctgt
     2401 aaaagggatc aaaagcaata ttaaggcaga aggtagttat accaaaatga aagaaaaatc
     2461 agtcggtaaa gacacaacta ccggtcactg ggaattagcc ggtctggtct ctgaaaagcc
     2521 ttttccggtt tatccgaacg gttttccgga agaggtaata aaaacttttg aaaaaaagat
     2581 aggaaaagaa gtattgggga ataaacctgc ttctggaact gtaattatag aggaattagg
     2641 agaagaacat ttaaatactg gtaaaccgat tgtctatacc tcagccgata gtgttttcca
     2701 aattgctgcc catgaagata taatttcagt tgaagaactt tatgagatgt gtgaagtagc
     2761 ccgcgagata ctgcagggtg aacacggagt agcgagggta attgcccgcc cctttgtggg
     2821 agaactgggc agctttacca gaacagatcg tagaaaagac ttttctttgg ctccaccgcg
     2881 tgcaactgtg cttgataagt taaaagctcg tggaatttct gtgagggcgg tgggcaagat
     2941 agaagatatt ttttccaata aagggattac agattcgaac cacaccgtag ataatatgga
     3001 ctcggttgat gccgtaattg aatatttaaa agaagataaa aagtc
//
LOCUS       DATGCJ010000133        12382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_61098, whole genome shotgun sequence.
ACCESSION   DATGCJ010000133 DATGCJ010000000
VERSION     DATGCJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61098"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VKN64_06670"
     CDS             complement(<1..1367)
                     /locus_tag="VKN64_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMA59958.1"
                     /translation="MEDIKSIEEINLKEIKNNTKYFKSPADWEDQIIYFLIVDRFSDG
                     KEDNRELFNPAEDFDSIKETDSIEEWHNWGEKWNGGNLQGVISKLDYLKKLGITVIWL
                     SPIFKQVSFQESYHGYGIQNFLKIDPHLGSKEDLKELVKEAHKRGIYVILDIILNHSG
                     DVFAYEEDIPKWTGDTYNIKGFRDEEGFPSIDIENKNIDEIWPNGAVWPKEMQDKDIY
                     ERKGEIQNWDYYPEYVQGDFMSLKTHFLGEYTEEEGFIPSKALNILTKAYKYWIAETD
                     IDGFRLDTVKHLEWGATKHFVREIHEFTKSIGKKNFYLIGEITGGFDFAYDTLNKTGL
                     NAALGINDIPHRLEGVAKGYTNPQEYFNIFSNTDILGEDDYKWFKDNVVTMFDDHDMV
                     SLGDNNKYRFAADEETAPLLLNSIFLNVTTLGIPCLYYGTEQGFDGSGDADKYIRECM
                     FGGKFG"
     gene            complement(1380..1883)
                     /locus_tag="VKN64_06675"
     CDS             complement(1380..1883)
                     /locus_tag="VKN64_06675"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMA59959.1"
                     /translation="MEKVKFNELSTEVMDAIKIGAFLTVKDDEELNTMTIGWGSIGVI
                     WGQPMFTVLVRHSRYTHKLIENTDQFTVSVPFGNKLKRETDYCGTHSGRDVNKFEELG
                     LELIEGKELDTPFIKGNDAHYECKIVYKQDMESDLLSDQTIKDQYYPQGDYHTIYYGK
                     IIGAYKE"
     gene            complement(1918..2424)
                     /locus_tag="VKN64_06680"
     CDS             complement(1918..2424)
                     /locus_tag="VKN64_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA59960.1"
                     /translation="MVDIIKKRRSIRSYKDKDVKDEEIYTLLEAAMLAPSAGNEQPWH
                     FIIIDNQEIINKIPDIHPYAKMITQVKKAILVCADLNETKYEDYWVQDCAAATENILL
                     KGVDLGLGTVWLGLYPDEERVAKFKNLFKLPADIIPFSLIPVGYPDQDKKTPDRFDKS
                     RIHRNKWK"
     gene            complement(2417..4162)
                     /locus_tag="VKN64_06685"
     CDS             complement(2417..4162)
                     /locus_tag="VKN64_06685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HMA59961.1"
                     /translation="MTIFVIDTFDQSMLSHGEVVTSIIEQQVPQFNIKRLNVKEKEHY
                     GISKYYQALDQIYNYKLQNTQEKVIVNISLGFYESEEYHEILIERLAELGVGVVAAAG
                     NDDSPIAFYPAAYEDEVIAVASIKGDQKTDYSNYGEYIDICAEGSFFTTISLPQFFSY
                     RASGTSFAAPRVSAFLSKIMASKKDMNLDKAVERLNQLTTPLDDELFEKGLLGKGKIS
                     NWKFLFNYNKVKLLYFYLLPGIVFLLIFYLLIKKMGLIAVPYLLLLLIVLGPFFIFMR
                     DYFLRSLTHQIITLENLRLTLVFIVSYLAVKILTSFEKYYLFIIYFVINSAAAAIMYF
                     TNLLDLYNYIIISMIMVILFYSYEYYLYRNKKDSQEIKDLETNSYKVLNKVKSNIIDK
                     AVFNEEKLTKIFTIYQITGKNHVKKAIIDILLARLRHIPFGFLYKNSESIIVKNYLFN
                     ISKENKEQVPVKELFEALKEDAYYIDKIFAAYSFDEIGEQLINEFGKRDININTLIKI
                     INYYNDSSIVDYLISIYDNYEKCWHRYLLAKTILRLADDKKRKELVKEFINDNCGLVK
                     EEAHYYRKKEGYSIG"
     gene            complement(4254..5357)
                     /locus_tag="VKN64_06690"
     CDS             complement(4254..5357)
                     /locus_tag="VKN64_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMA59962.1"
                     /translation="MVIISNIFNSKFFKISLGTILILIILILLRQVAFFTRAFQIVTS
                     LFIIPMLLGGFLYYLMRPLVVFLTKYLRFKGIAIFISFLLIITLIVFIVYFGGNIITQ
                     QTRNIISQFQDYYYSQTPTPEEDNIFFEVGTQIMEYLERFNIQERLSTFLENMVEVVR
                     NNIFGIFTTLTNIGTVIVLIPFVVYYLLKDDHHMKTSFLAYFPPEKKEHMDKMLKKVD
                     QTLSRYIRGQVLVALMLGILTYIGFIIIGVPNAIILSAIVMVTSFIPFLGPILGILPA
                     VFIGISHSFVMVFQIILVLIIVQQIEGNVIQPKVQGKRLQIHPLIVIFMVLIFITLFG
                     FIGALYAVPAYAVARVLVQEYHGKYFLEENNNE"
     gene            complement(5351..6226)
                     /gene="hslO"
                     /locus_tag="VKN64_06695"
     CDS             complement(5351..6226)
                     /gene="hslO"
                     /locus_tag="VKN64_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HMA59963.1"
                     /translation="MDDYMIRVLTKNKQIRALAVRSTNLVSKAQKLHKTTPVATAALG
                     RALSAVAMMISLEKSGQKIGLRIIGEGPIDRIIAEANMDGEVRGYVGNPQIKVMINEQ
                     NKLDVKKAVGKGELYVRKMMGLKEPYESKIKLVSGEIGEDLTYYYTQSEQVPSSVGLG
                     VLVDADSSVKAAGGFIIQLLPETPDETISQLEKNLKNIGSVSRIIEEGKTPEELLDIV
                     LAGFDYDITQVKEVEYKCNCNKERGLEIVNTLSAEDIKKSLEEDGYVEVTCHYCNSKY
                     RYNEEYIKEHLEREW"
     gene            complement(6219..7457)
                     /locus_tag="VKN64_06700"
     CDS             complement(6219..7457)
                     /locus_tag="VKN64_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMA59964.1"
                     /translation="MKYGLILEGGGAKGSYQIGAVRALNEMGYEFKGVAGTSIGALNG
                     ALIVQSDLDKAYDLWYNISPEKIFDIRQEALDKIKKLNFDKKDLTYLWNQVKNIVDNK
                     GLDISLIEDIVKENVEEDKIRNSDKDFGIVTVSLSDMKPVKIYIEEIPEGRLIDYLLA
                     SSNLPTFKQEKRNGKYFLDGGFYDNLPVDMLVDKGYENIIVIRTHGLGITRDIKDKEK
                     LNIKYIEPDVDLGNILDFDTKVARRNLRLGYLDAKRLFNDNIKGHDYYIKIEKDTDYF
                     LNLLMNIPDKSIKRIADFLGYEEMNPRRALFEVIIPRLAGFLDLEEEHDYVDIMVAVL
                     EVIAKKVKLEKLKIYGFDDFVKEISEKYEPKKVDEVNVPKFVKKSEILSKTIKDKIID
                     LVIAELFDNIINRGGEYNNG"
     gene            7602..8900
                     /gene="hflX"
                     /locus_tag="VKN64_06705"
     CDS             7602..8900
                     /gene="hflX"
                     /locus_tag="VKN64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMA59965.1"
                     /translation="MGTVRKKRWITIINFKKDEKDLYKVKDKKENALLVSNDKTSIKE
                     LGQLADTSGVHTIKYFTYRSDKINPAYYIGKGNLEKIKDIIENFDINLVIFDNQLSPA
                     QLRNIEEELDAKILDRNQLIMDIFALHAKSKESKIQVELAQLEYLLPRLVGRGKELSR
                     LAGGIGTRGPGETKLEVDRRRIEKKIYRLKEKLKDIKGDRQVQRKSRKDPIVSLVGYT
                     NAGKSTILNLLTGDNSLVEDQLFATLDSRARSYELPNGKNIIMTDTVGFIKNLPHQLI
                     ASFRSTLEEIKEADLILHIIDASDEEVDNKIKVVENELVELGIQDKPILKVFNKIDLI
                     DSHTKKDLEIQHPEAVFISATESFNKDKLIERLNNFVEARMDRYSLEIPYDKASIVET
                     LYNRGIVEKEKYKNEHIFIDAYIPSTLSKKLEKYIVNSKN"
     gene            complement(8897..9817)
                     /locus_tag="VKN64_06710"
     CDS             complement(8897..9817)
                     /locus_tag="VKN64_06710"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMA59966.1"
                     /translation="MQYVRENIEFEDMVFAFHKWKPDISNYKGIIQIIHGMAEHVKRY
                     NEFAEYFTEKGFLVCGIDHLGHGETVQLNEGKYGYFAEKEGWVKVVNSNKYISKILKD
                     RHSDLSLFMFGHSMGSIIARYYLIGEKVVDGLIISGIMEYQEILMDFVKVLAKLEKAM
                     KEDKSANVIDNYLLDLIYNRKFDKDSSKNWISSLKEEVENYDKDKNCGFKITNQMYID
                     MMNGLKTIYNIEKNKEISKDIPLFIMSGQDDPVSNYGKDVKKLFNKYKEANVKDIKYK
                     IYSNIRHEVIRDKRNQDVYNDLEKWISDHI"
     gene            complement(9817..10626)
                     /locus_tag="VKN64_06715"
     CDS             complement(9817..10626)
                     /locus_tag="VKN64_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMA59967.1"
                     /translation="MDFKVTLIQSNIKIGDEKYNFKKTKDFLHQAANLNSDVVVLPET
                     WLSGFGQQVFDNIEQYAKKENSEHIQYLRDFAVDKELHIVAGTILEKGRDNNIYNNLY
                     FIDNKGNIGAKYAKMHLYSPGGENEVVTAGSSISTYQTEFGKFGFMVCYDIRFPELSR
                     LYALEGIDVLYVTANFNDPKRDHWLNQLKARALENQIYVAAVNRSGKNYFGNSVLVNP
                     AGEIILNAGRSEGIYSGFIDLSFIKAVRQEFPLFNDRREDVYNLSLKRGES"
     gene            complement(10638..10985)
                     /locus_tag="VKN64_06720"
     CDS             complement(10638..10985)
                     /locus_tag="VKN64_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HMA59968.1"
                     /translation="MYRINSYKFGQIEINNQSYQNDVIIYPDQLKDNWWRNEGHRLHE
                     SDIDKLSDYQPDLVIIGTGASGRMKVDHKLKQYFDEHNIKYYISTTNDAVKKHNEMID
                     KKQQVLTALHLTC"
     gene            complement(11005..11682)
                     /locus_tag="VKN64_06725"
     CDS             complement(11005..11682)
                     /locus_tag="VKN64_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMA59969.1"
                     /translation="MYIIVAGGGVVGSSITKRLVSEHDVVVIDEDQNVAEKIYAKYGA
                     VSVVGNATNIDVLKDAGIEKADVAIGVMDSDSNNLSFSLLAKNFGVEKILVRMRNPEY
                     KNAYEMAGATNVGAVNEMLVDNFIIDIEEPNIRKVVSIGEGNAEVSIVTLPEDFKKSG
                     STVSDIVNSEGFPEECVIAGIFDQDSRKLRIPRGSTKLYKNDQLFLVATKENMEKAAE
                     FLLNDKK"
     gene            complement(11696..>12382)
                     /locus_tag="VKN64_06730"
     CDS             complement(11696..>12382)
                     /locus_tag="VKN64_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMA59970.1"
                     /translation="VLKGDIKEVFTNFEMKYFWGIIGTVMLLILTGKLFNQSLNFANL
                     EMVFRKSLFQVVSVMTTTGYGTESLTSNFFPAIARQLFLFLMLIGGCVGSTAGGIKVI
                     RFAVLNKLFGREIKKFYLPKRAVMPLRIEKKLIPRDEIYKISALFFFWLLIILVGGII
                     TALFSDLDAFQSISGMFSAMGNIGPFYFSVGKMASLSPIIKLTYIFGMLAGRLEILPI
                     FIIFTRKAWK"
BASE COUNT         4041 a         2389 c         1636 g         4316 t
ORIGIN      
        1 ccaaatttac cgccaaacat acattctcta atgtatttat cagcatctcc actgccgtca
       61 aatccctgtt cagtcccgta ataaagacag ggaataccca atgtagttac atttaagaat
      121 atactgttta aaagcaaggg cgcggtctcc tcatctgccg caaacctata tttattatta
      181 tcacctaaac taaccatatc atggtcatca aacatagtta caacattatc tttaaaccat
      241 ttataatcat cttcacctaa gatatctgtg ttagaaaata tattaaagta ttcctgagga
      301 ttagtatatc ctttggcaac tccttccaat cggtggggga tatcattaat tcccagggca
      361 gcatttaaac ctgttttatt taaagtatca taggcaaaat caaatcctcc tgttatctca
      421 cctattaaat aaaagttctt tttacctata cttttagtaa attcatggat ttcgcgcacg
      481 aaatgcttag ttgctcccca ctctaagtgc tttacggtat ctaacctaaa gccatcaata
      541 tcagtttctg cgatccagta tttataggct ttggttagta tatttaaggc ttttgaaggt
      601 ataaagcctt cttcttctgt atattctccc agaaaatgag tttttaggga catgaagtcg
      661 ccctgtacat actcgggata gtaatcccaa ttttgtattt ctccttttcg ttcataaata
      721 tctttatcct gcatttcttt tggccaaacg gctccattcg gccatatttc atcaatattt
      781 ttgttttcta tatctataga gggaaaaccc tcttcatctc taaaaccctt tatattataa
      841 gtatcgccgg tccattttgg gatatcttct tcatatgcaa acacatctcc agaatggttt
      901 aagataatat ctaagatgac atagataccc cttttatggg cttctttgac taactctttt
      961 agatcttctt tactgcctaa atggggatct atctttaaaa agttttgaat gccgtagcca
     1021 tggtaagatt cttgaaatga aacctgttta aagatagggc ttaaccaaat aacagttatc
     1081 cccaacttct ttagataatc aagtttggag ataaccccct gcaggttacc cccattccat
     1141 ttttcacccc aattatgcca ttcttcaata gaatcagttt ccttgattga atcaaaatct
     1201 tcagcagggt taaacagctc tctattatcc tctttaccgt ccgaaaaacg gtcaacaata
     1261 agaaaatata tgatttgatc ttcccaatcc gccggggatt tgaagtactt tgtattattc
     1321 ttaatttctt ttagatttat ttcttcgata cttttaatat cttccatagt tatcaatcct
     1381 tattctttat atgctccaat tattttacca taataaatag tatggtaatc tccctgagga
     1441 taatattgat ctttaatagt ttgatcagat aacaaatcag attccatatc ctgtttatag
     1501 actattttac actcataatg ggcatcatta cctttaataa aaggtgtatc aagttcttta
     1561 ccctctatta attctaaccc taattcttca aacttattaa catctctgcc agaatgagta
     1621 ccacaataat cagtttctct ttttaatttg tttccaaaag gtactgaaac ggtaaactga
     1681 tctgtatttt caatcaactt atgtgtatac ctggaatggc gaaccagaac agtaaacata
     1741 ggttgacccc aaataactcc aatgctgccc caaccaattg tcatcgtatt taactcttca
     1801 tcatctttga ctgttagaaa agctcctatc ttaattgcat ccataacctc tgtagatagt
     1861 tcattaaatt taactttttc cataaatatt attacacctc tccttttctc ctctacttta
     1921 tttccattta tttctatgaa ttctactttt atcaaacctg tcgggtgttt tcttgtcttg
     1981 atcaggataa ccaacaggaa ttaatgaaaa tggaattata tcagcaggta gtttaaatag
     2041 atttttaaac ttagcaactc gttcttcatc gggatatagg ccgagccaaa cagtgcctaa
     2101 gccgagatct actcctttta gtaaaatatt ttcagtagct gcagcacagt cttgaaccca
     2161 gtaatcttca tatttagttt catttaagtc agcacagact aaaatggctt ttttaacttg
     2221 agttatcatt tttgcataag gatgaatatc tggaatcttg ttaattattt cctgattatc
     2281 aattattata aaatgccagg gttgttcatt cccggcagaa ggagcgagca ttgccgcttc
     2341 taataaagta tatatttcct catctttaac atccttatct ttgtatgatc ttatactcct
     2401 tctcttcttt atgatatcaa ccaattgaat acccctcctt tttacgataa taatgagctt
     2461 cttctttaac taatccgcag ttgtcattga taaattcctt aaccaattct tttctttttt
     2521 tatcatctgc caatcttaat atagtttttg ctaataaata cctatgccag catttttcgt
     2581 aattatcata aattgaaatt agataatcaa caatagagct gtcgttataa tagttaatga
     2641 tctttattaa ggtattaata tttatatctc gcttaccaaa ttcattaatc agttgttccc
     2701 ctatttcatc aaatgaataa gcggcaaata ttttatctat atagtaagca tcttccttca
     2761 gggcttcgaa cagctcttta actggtactt gttctttgtt ctccttgcta atattgaata
     2821 gataattctt tactattata gattctgagt ttttatataa aaatccaaag ggtatatgtc
     2881 ttagccttgc cagtagaata tctataattg ctttttttac atgattcttg ccagtaattt
     2941 gatatatagt aaatatttta gttagttttt cttcattaaa gactgcttta tcgataatat
     3001 tagactttac tttattcaat actttatagg agtttgtttc cagatcctta atttcctggg
     3061 aatccttttt atttctataa agataatatt catagctata aaataatatt accattatca
     3121 tactgataat aatgtaatta tataggtcta ataaattagt aaaatacata atagcagcag
     3181 ctgcagaatt gataacaaaa taaattataa atagatagta tttttcaaag ctagtaagaa
     3241 ttttaacagc taaataagat acaataaata ccaaagttaa tctcaagttc tcaagagtaa
     3301 ttatttgatg tgttaaggac cttaaaaaat aatctctcat aaagatgaaa aacggcccta
     3361 acactattaa taataataat aaataaggta ctgcgattag acccattttc ttgataagta
     3421 gataaaagat tagtaaaaat actatgcccg gtagtaaata aaagtataat aatttaacct
     3481 tattataatt aaatagaaac ttccaattac ttatcttacc ttttcctaaa agtccttttt
     3541 caaaaagttc atcatctaaa ggagtggtta attgatttaa tctttctaca gccttgtcta
     3601 aattcatatc tttttttgaa gccattattt tggataaaaa agcacttacc cgcggagctg
     3661 caaaggaagt cccactcgct ctataactaa aaaattgagg taagctaatc gtagtaaaaa
     3721 agcttccctc agcacaaata tctatatatt caccataatt ggagtagtcg gttttttgat
     3781 ctcccttaat actggctact gcaataactt catcctcata agccgctgga taaaaagcta
     3841 ttggactatc atcattacca gctgccgcaa caacacctac tcccaattca gctaatcttt
     3901 cgataagtat ttcatggtat tcttctgatt cataaaatcc caaactaata tttactatta
     3961 ccttttcttg agtattttgt aatttatagt tataaatttg atctaaggct tgatagtact
     4021 tgcttatccc ataatgctct ttttctttca catttaatct tttaatatta aactggggaa
     4081 cttgctgctc tattatagaa gtcactactt ccccatggct taacatactc tgatcaaagg
     4141 tatcgataac aaatatggtc acagactcat gctcctgaaa actgtaaacc acgggataaa
     4201 tataacctat taacactaat attaaaataa tgaataaaat ccaaacatcg cgtctattca
     4261 ttgttatttt cttctaaaaa gtacttacca tggtattcct ggaccagaac tctagccact
     4321 gcataggctg gaacagcgta taaagcccca ataaatccaa agagagtaat gaagatcaat
     4381 accatgaaaa tcactatcag aggatgaatt tgtaatcttt taccctgtac tttaggttga
     4441 atcacattac cttcaatctg ctgaactatt atcaatacta atattatctg aaaaaccatt
     4501 acaaaactat gtgatattcc aataaataca gccggtaata tccctaaaat aggccctaaa
     4561 aatggtataa aggaggtaac catcactata gctgataaga taatagcatt aggtacccct
     4621 atgataataa agcctatata cgttaaaata ccaagcatta gggcgactaa aacctgacct
     4681 ctaatatagc gagagagagt ttgatcaact ttttttagca tcttatccat atgttctttc
     4741 ttttcaggcg ggaaataagc taaaaatgag gtcttcatat ggtggtcgtc ttttaaaagg
     4801 tagtaaacta caaaaggtat taagacaata actgtaccga tattagttaa cgtagtaaat
     4861 ataccaaata tattatttct cactacctca accatatttt caaggaaagt agaaagtctt
     4921 tcctggatat taaacctttc taaatattcc atgatttgag ttcctacttc aaaaaagata
     4981 ttatcttctt caggtgtagg tgtttgtgag taataataat cctgaaactg acttattata
     5041 ttacgggttt gttgggtaat tatattgcct ccaaaataga caataaagac tattaatgtg
     5101 ataatcaata aaaagcttat aaatatagca atccctttaa accttaagta cttagttaaa
     5161 aaaactacta gaggcctcat taaataataa agaaagcctc ctaaaagcat tggtataata
     5221 aaaagggaag tgactatttg gaatgctctt gtaaaaaacg caacctgtcg tagtaaaata
     5281 agtataatta aaatcagtat agtacctaaa ctaattttaa aaaacttact attaaagata
     5341 ttactaatta tcaccactcc ctttcaagat gttctttgat atactcctca ttatatctat
     5401 atttagaatt acagtaatga caggtcactt ctacataccc gtcttcttct aatgattttt
     5461 taatatcctc tgcacttaag gtatttacga tttctaaacc tctttcctta ttacagttac
     5521 atttatattc tacttccttg acctgggtaa tatcataatc aaaacctgct aaaactatat
     5581 caagtaattc ttccggagtt ttaccttctt caataattct gctaacagaa cctatatttt
     5641 tcaaattctt ttctaattgt gaaatagtct catccggggt ttcgggtaat aactgaatga
     5701 taaagccgcc tgctgctttg acagaggaat ctgcatccac taaaacgccc aaaccgactg
     5761 aagaaggtac ttgttccgat tgggtatagt aataggttag atcttctcct atttccccgc
     5821 taacaagttt tatcttgctt tcatagggtt ctttgagccc catcatcttt cttacataaa
     5881 gttctccctt gccaaccgct tttttaacat ctaacttgtt ctgttcattg atcataactt
     5941 tgatttgggg gttgccaaca tatcccctaa cttctccgtc catattagct tctgcaatga
     6001 ttctatcaat aggaccttct ccaattattc tcaggccgat cttctgaccg gacttttcta
     6061 aagaaatcat cattgctaca gccgaaaggg cccgacctaa ggcagctgtt gcaacaggag
     6121 tagtcttatg tagcttttgt gctttactta ctaaattggt agaacgaact gctaatgccc
     6181 taatctgttt gttctttgtt agcactctta tcatataatc atccattatt atactctcct
     6241 cccctattaa taatattatc aaataattca gctataacga ggtcaattat tttgtcttta
     6301 atagttttag aaagtatctc gcttttttta acaaactttg gcacattgac ttcatctact
     6361 ttttttggtt catatttttc agaaatttct ttcacaaaat catcaaatcc atatattttt
     6421 aatttttcta gtttaacttt cttagctatt acttccagga cggccaccat aatatcaaca
     6481 taatcatgtt cttcctcgag atctaaaaag ccggccaacc tgggaattat tacttcaaat
     6541 aaggcacgcc tggggttcat ctcttcatag ccgagaaaat cagcaatcct ttttatgctc
     6601 ttatccggaa tattcatcaa taaatttaag aaataatcgg tatctttttc gattttaata
     6661 taataatcat ggcctttgat attatcatta aatagacgtt tagcatctaa ataacctaac
     6721 cttaaattac ggcgcgctac ctttgtatca aagtccagga tattaccgag gtctacatct
     6781 ggttcgatat attttatgtt taacttttct ttgtctttaa tgtctcttgt tattcctaat
     6841 ccgtgtgttc ttattactat aatattctca taccccttat caaccagcat atcaacgggg
     6901 agattatcat aaaaaccacc gtccaaaaaa tattttccat ttcttttttc ctgtttaaat
     6961 gttggtaaat ttgaactggc taacaaataa tctattaatc taccttccgg tatttcttct
     7021 atgtagattt taaccggttt catatcagat agagaaacag ttacaatacc gaaatcttta
     7081 tcagaatttc ttattttatc ttcttccaca ttttctttca ctatatcctc aattaatgag
     7141 atatccaaac ctttattatc cacaatattt ttaacctgat tccatagata ggttaaatcc
     7201 tttttatcaa aatttaactt ttttatttta tccaatgctt cctgcctgat atcgaaaatc
     7261 ttctcaggac ttatattata ccaaaggtca taagccttat ccaaatcgga ctggactatt
     7321 aatgcgccat ttaaggcacc tatagaagta cctgctacac ctttaaactc atagcccatc
     7381 tcatttaaag cgcgcacagc cccgatttga taggaacctt tggcccctcc tccttctaat
     7441 attaaaccat acttcatagt atcacttcct aaaaatttca ttaaaatata tctaataaac
     7501 aagctacaaa aatttattgt aacctattat aaataattca atatatcgtt tagttattcc
     7561 ttcttaaaat actatttata tatactaaat tatttgatat aatgggaaca gtaagaaaaa
     7621 agaggtggat tactattata aactttaaaa aggatgaaaa agatctatat aaggtcaaag
     7681 ataaaaaaga gaatgcccta ttagttagta atgataaaac atctattaaa gaattaggac
     7741 agttagccga tacttccggc gttcatacta ttaagtactt cacttaccgg agtgataaaa
     7801 tcaatccagc ctattatatc ggcaagggta atctagaaaa aatcaaagat attattgaaa
     7861 attttgatat aaacttagta atctttgata accagctctc tcctgctcaa ctgcgtaata
     7921 ttgaagaaga acttgatgca aaaatattag accgtaacca gttaattatg gatatttttg
     7981 cccttcatgc caaaagtaaa gaaagtaaga ttcaggtaga acttgcccaa cttgaatatc
     8041 tactgccccg cttggtaggt cgcggtaaag aactctcaag attagcaggt gggattggta
     8101 ccaggggtcc tggtgaaaca aaactagagg ttgaccgccg tagaatcgaa aaaaagatct
     8161 atcgactaaa agaaaaatta aaagatatta agggagaccg acaagtacag agaaaaagta
     8221 gaaaggatcc gatagtatct ttagttggtt atacaaatgc cggtaaatca acgatattaa
     8281 accttttaac cggtgataac tccttagtag aagatcaact ttttgccacc ctagattcac
     8341 gggcaagaag ttatgaacta cccaatggaa aaaatattat tatgacagac acagttggtt
     8401 tcattaaaaa tttacctcac cagctaatag catcgtttag gtctacctta gaagaaatta
     8461 aagaagcaga cctaatctta catataattg atgccagtga tgaggaagtt gacaataaaa
     8521 ttaaagttgt agaaaacgaa ttagttgaat taggtattca ggataaaccg atccttaaag
     8581 tatttaataa aattgattta atagattccc atactaaaaa ggaccttgaa atccagcatc
     8641 cagaggctgt tttcatctca gccacagaaa gttttaataa agataaatta atagaacgtt
     8701 taaacaattt tgtagaagca agaatggata gatactcact tgaaatcccc tatgataaag
     8761 caagtatcgt ggaaactcta tataataggg gaatcgttga aaaagaaaaa tacaaaaatg
     8821 aacatatatt tattgatgct tatataccct caactttatc taaaaaacta gaaaagtata
     8881 tagttaacag taaaaattaa atatggtctg aaatccattt ttctaaatca ttatagacat
     8941 cttgatttct tttatcacga atcacttcat gtctgatgtt agaataaatt ttgtatttaa
     9001 tatcttttac gttagcttct ttatatttgt taaataactt tttgacatct ttcccgtaat
     9061 tactaaccgg atcatcttga ccactcatta taaataaagg gatatccttt gatatctctt
     9121 tatttttttc tatattatat atggtcttta atccattcat catatctata tacatctgat
     9181 ttgttatttt aaaaccacag tttttatcct tatcataatt ttcaacctct tcttttaaac
     9241 tacttatcca atttttacta ctatctttat cgaattttct attgtagata agatctaata
     9301 gataattatc tataacatta gcacttttgt cctctttcat tgccttctca agttttgcaa
     9361 ggacttttac aaagtccatt aatatttctt ggtactccat tatgcccgaa attattaatc
     9421 catctacaac tttttctcct attagataat atctggcaat tatagaaccc atgctgtgtc
     9481 caaacatgaa aagtgataaa tctgaatggc gatcctttaa tatttttgat atatatttat
     9541 tggaatttac taccttaacc caaccctctt tctcagcaaa atatccatac ttaccttcat
     9601 ttaattggac ggtttcgcca tggcctaaat gatctattcc acagactaaa aaacctttct
     9661 ctgtaaagta ttctgcaaac tcgttgtatc tctttacatg ttcagccatc ccatgaatta
     9721 tttgaataat acccttgtaa ttggaaatat caggtttcca tttgtggaag gcaaaaacca
     9781 tatcttcaaa ttcaatattc tctcttacat actgcattaa ctttcacctc tttttaaact
     9841 caaattatat acgtcttctc ttctatcatt aaagaggggg aattcttgcc ttactgcttt
     9901 aatgaaagaa agatcaataa atccactgta aattccttca ctacgaccag catttaagat
     9961 aatttcaccg gctggattaa caagaactga attaccaaaa taatttttcc cgcttctatt
    10021 aactgctgct acatatatct gattttctaa agctcttgct ttaagttggt ttaaccaatg
    10081 atctcgcttg ggatcattaa aattagcagt tacgtacagt acatctattc cttctagggc
    10141 ataaaggcgg cttaactcgg gaaacctaat atcatagcag accataaaac caaacttgcc
    10201 aaattcggtt tgataggtac taatactact gcccgcagtt accacttcat tttctccgcc
    10261 cggggaataa aggtgcattt ttgcatactt ggcccctata ttacctttat tatctataaa
    10321 ataaaggtta ttatatatat tattgtccct acccttttcc aggatcgtgc ccgctactat
    10381 atgaagttct ttatcgacag caaaatccct tagatactgt atgtgttcac tattttcttt
    10441 tttggcatat tgttctatat tatcaaatac ctgctggcca aatcccgaaa gccaggtttc
    10501 tggtaagaca accacatcag agtttagatt agctgcctgg tgtaaaaagt cctttgtctt
    10561 tttgaagttg tatttttcat cacctatttt aatattactt tgaatcaatg ttaccttaaa
    10621 atccatctaa gttcctccta acaggttaaa tgaagagccg taagaacttg ttgttttttg
    10681 tcaatcattt cattatgctt tttaacagca tcattggtcg tagagatata gtacttaata
    10741 ttgtgctcat caaaatattg ttttaacttg tgatcaactt tcatccttcc actggcccct
    10801 gttccaataa ttacgagatc aggttggtag tcagataatt tatcaatatc actttcatga
    10861 agcctatgtc cttcatttct ccaccaatta tcctttaact gatcaggata aataataaca
    10921 tcattttgat aagactgatt atttatctcg atctgtccga acttataaga attaatacga
    10981 tacacagtaa ataccccctt gaaattattt tttatcattc aagagaaatt cagctgcctt
    11041 ttccatattt tctttggtcg ccactaaaaa taactgatca tttttatata acttagtact
    11101 accacgtgga attcttaact tacgtgaatc ttgatcaaat atgccggcaa ttacacattc
    11161 ctcagggaac ccttcactat ttactatatc agatactgta ctacctgatt ttttaaaatc
    11221 ctctggtaga gttacaatag aaacctcagc attaccttcc ccaatagaaa ccacttttct
    11281 aatatttggt tcttcaatat caattatgaa attatcaact aacatttcat taacagcacc
    11341 tacattagta gctccggcca tttcatatgc atttttgtat tctggatttc tcattctaac
    11401 caaaatcttt tctactccaa agtttttagc taaaagtgaa aaagacaggt tattagaatc
    11461 agaatccata actccaattg caacatcagc tttttctata cctgcatctt taaggacatc
    11521 aatattagta gcattaccta ccacactgac cgccccatat ttagcatata tcttttctgc
    11581 tacattctga tcttcatcaa ttactacaac atcatgctca ctgactagac gtttagtaat
    11641 ggaacttccc acaactcctc cacctgcaac tataatatac atttataaat tccccctatt
    11701 tccaagcctt gcgtgtaaat atgataaata tcggtaatat ctccagtcgg ccggccaaca
    11761 taccaaagat ataagttagt ttgataatcg gtgataatga ggccattttg ccgactgaga
    11821 agtaaaatgg tcctatatta cccattgcag aaaacatacc tgaaatcgat tgaaaagcat
    11881 ctaaatctga gaataaagct gtaattattc cccctactaa tattatcaat aaccagaaga
    11941 aaaacagggc cgaaatctta tatatctcat ctctgggtat taatttcttt tctatcctta
    12001 aaggcattac tgctcttttc ggtagataaa acttcttaat ttctcttcca aacaatttat
    12061 ttaaaactgc aaatcttata accttaatac cacctgcagt agaacccaca caaccaccga
    12121 tcaacattag aaagagaaat aactgccggg ctatcgcagg aaaaaagttc gaagttaaag
    12181 attcagtccc atagccggtc gtagtcataa cagaaactac ctggaaaaga ctttttctaa
    12241 agaccatctc cagattagcg aaattaaggg attgattgaa cagtttgccc gttaaaataa
    12301 gtaacatcac tgtgccaata attccccaaa aatatttcat ctcaaagttt gtaaatactt
    12361 cttttatatc tcctttaagc ac
//
LOCUS       DATGCJ010000134        19017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_107538, whole genome shotgun sequence.
ACCESSION   DATGCJ010000134 DATGCJ010000000
VERSION     DATGCJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 19017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19017
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107538"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /gene="recA"
                     /locus_tag="VKN64_06735"
     CDS             <1..910
                     /gene="recA"
                     /locus_tag="VKN64_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMA59971.1"
                     /translation="LPLDIALGVGGIPKGRIIELYGNESSGKTTLGLHIIAEAQKQGG
                     IAAFIDAEHALDPRYAENLGVNIDDLLISQPNNGEEALEIAEALVRSNAIDLVVIDSV
                     AALVPKAELEGEMGDTHVGLQARLMSQAMRKLSGAISKSDTACIFINQIREKVGVMFG
                     NPETTPGGRALKFYSSVRIELRRSQTIKDGDEIIGSHARAKVVKNKVAAPFRKAEFDI
                     MYGTGISRTGCVLDMGEDTGIIDRAGSWYSYGDVRLGQGRENSKEFLKENEDILKEIE
                     HKIKVKVGLIEDEEIEEEETKEEKEA"
     gene            1049..2593
                     /gene="rny"
                     /locus_tag="VKN64_06740"
     CDS             1049..2593
                     /gene="rny"
                     /locus_tag="VKN64_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HMA59972.1"
                     /translation="MKIIYNTLLIVAISLVPGLIIGYFIRKFIAEARIQSAEEESRKI
                     LEEAEREAESKIREITLEAKEKAQKIREEANRESQKRRNELQRLEDRLMHREETLDKK
                     GETLEKKEQKLKKQQKELEEEQQEIEDIKQQEIEKLEKISNLSHSEAKEYLLSKMEDE
                     LDHEYAKMIKEKEREAKEEANKKAREIISLAIQRCAGDYVSETTVTVVDLPNDEMKGR
                     IIGREGRNIRALESLTGIDLIIDDTPEAVVISGYDPIKREIARIALEKLIVDGRIHPA
                     RIEEMVEKAEDELDNQIKEVGEQATFDSGVHGLDSQLVKYLGRLKYRTSYGQNVLQHS
                     LEVSFLAGVMADELGADSTLAKRGGLLHDIGKAIDHEVEGPHIDIGVNLARKFGESED
                     VIHTIEAHHGDVDFQSVEAVLVAAADAISAARPGARKETLEAYIKRLEELEEIGQSFD
                     GVENAYAIQAGREIRVIVQSEQVDDTTASKLSRDIKKEIEDNLNYPGQIKIVVIRETR
                     QIEYAK"
     gene            2655..3434
                     /locus_tag="VKN64_06745"
     CDS             2655..3434
                     /locus_tag="VKN64_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HMA59973.1"
                     /translation="MNILFIGDIVGRAGRRSIRKLMDDIVKNQDIDFVLANGENAAGG
                     FGLTEDTAEELYSYGIDCMTMGNHTWGNKNIYEFIDDNEKLIRPLNFNERVPGRGYNT
                     YQIKDKNITVINLIGQVFMGNHNSPVEAFDRVYEKIKDKSDYIIVDFHGEATGEKRAF
                     AEYVKGKVTLVVGTHTHVQTNDEQILDKGTGYITDLGFSGACDSILGMKKEKVLYRLK
                     TKMPKRLEVATGQVQLSGIFATFNQKGETTAINSFIVRDNP"
     gene            3528..3788
                     /locus_tag="VKN64_06750"
     CDS             3528..3788
                     /locus_tag="VKN64_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HMA59974.1"
                     /translation="MDVLKVSADSDPNSVAGALAGVLREQGSAELQAIGAGALNQGVK
                     AVAIARGYVAPSGIDLICIPAFTDIEIDGEERTAIKLIIEPR"
     gene            3883..4740
                     /locus_tag="VKN64_06755"
     CDS             3883..4740
                     /locus_tag="VKN64_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA59975.1"
                     /translation="MFVDLHLHTTYSDGSLTPEKLIKRAKEIGYSAIAVTDHDTLKGV
                     KPALNKAEKNNIEVVPGIEFNTSLNGYDVHILGYYIDLENKYLKNLLDKIKKERRERI
                     EKMVELLKGLYGYEISLQEIKNISENNIMGRAHIARLLTEKGYVQDWKEVFNKYIGRG
                     RPAYVDRNKITPFKAVDIIKKADGIPVIAHPGLIKNNQIVQQIINYGVAGLEVYYLEH
                     SAEQIEYYKELAQSNELLITGGSDCHGPKNKDGLKLGKIRLDYKYLAELKKYKTIVND
                     KAYKKDLLS"
     gene            4812..5456
                     /locus_tag="VKN64_06760"
     CDS             4812..5456
                     /locus_tag="VKN64_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMA59976.1"
                     /translation="MYNQIYNFISLGGPMAIPLIICSIFSVAVIIEKAVFFIRRKDNS
                     LRLLKRIELKVENDDIIGALNELKGEKGPNAKLLSTALSHYEEDPDRIREVLQAVGED
                     EIKKMEKHLPVLDVVAMISPLLGLLGTVLGIISSFNILGTAAGAANPAQISSGIAAAL
                     ISTAMGLVIAIPTAIFYSYYINKVEEKSHEMNLSMIDIMDVITDRSERDVQTYT"
     gene            5437..5841
                     /locus_tag="VKN64_06765"
     CDS             5437..5841
                     /locus_tag="VKN64_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMA59977.1"
                     /translation="MFKPTHKKKTNINIIPMIDVIFFLLVFFMLFTSFRTNPYGMDMK
                     LPKAVTVTEQKQENFIININENGEFFYQGDNLPMSKISELAKEKYQNNQNLVVIINAD
                     SNTKYSSIVTVMDSVRQVGIYNLALAAEKKGS"
     gene            5854..6723
                     /locus_tag="VKN64_06770"
     CDS             5854..6723
                     /locus_tag="VKN64_06770"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMA59978.1"
                     /translation="MFNNDRSKLFLYVTLSLVIHLLVFYFFPFGYLQGFSSEEASGDN
                     FGYVQYVEFRSQTNQRVEPPEDPEPQPAEEPEEEPEEEEEEEEPEPEPEPEPEPEPEP
                     EPEPEPEPEEQTTDSDVEIAQENEPEEEVEETQTNQDQEVISSEESDTEIEVEKKEEA
                     NQEQQEQEPEPEPEPEPEPEPTPPPPPTAGELIGLSPKPVYPKYLVSEAKSGKVGLSV
                     KVDQNGEVENIMVTNSSNTESMDRNAKLTVENGWQFKSYKQRYQINVTVNFNIDENGN
                     PNVDVEIGNVNFL"
     gene            6746..8722
                     /locus_tag="VKN64_06775"
     CDS             6746..8722
                     /locus_tag="VKN64_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HMA59979.1"
                     /translation="MKNRQRLIVTIILLVSFLFISVLTSAQSDVFTEVGSNNNLRLGN
                     DYIVIFINQDDNAKGRFAIETTGGAPMEPADDNKPLVYGRPKPWTSYTTLKINNVNYV
                     FGGSTKRRAGDDANYGEVTEGPVIKDNQIYTSTLIDGIKVEQILSIVKSSTTGLFDTA
                     QIKYRLINNSNQEKEVGLRIMLDTMLGQNDGAPFRLADKAVTTDTLYLQGELPRFWQA
                     FDSITNPTVTSQGTFRGPGVTVPDKVYFADWGSLADGDWNFDFNPGEEFVRKGEYEID
                     SAIAIMWEPKLLAPNEEVTYVTNYGLGGIKVVPGLLALGVTSPAEFTFDENNQSFPVV
                     AYVENTSEITAENVKVEIDLPQQFTAANPVKEIGDLKSKDTAQVSWQVRPAGEEIPGN
                     MTYRVKVSADNTDSNEVEREIRFVGPPAIEAEVTLEENLRKEKGEIKPNPFDMSVKFT
                     NIGGSTLFDAQTELALPPGLTLADKEVNRKYLGYLESGESIDVNWSVRVLNVTGEMPY
                     SINITGLNNYNKTKREDLDIPELEPFVYLEKVEFGESYQTYNLIGQNLATVNSLKLNL
                     SYNPAAYRINYTFPGDMFVQNDKIILWEDPVISDGRIEMNEILPDNAESGVIATLQFS
                     KKSENPLNLTIDSIEAMDEKNNEVDIKYENLKEE"
     gene            8730..9557
                     /locus_tag="VKN64_06780"
     CDS             8730..9557
                     /locus_tag="VKN64_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HMA59980.1"
                     /translation="MKRFRNYLVFTLIFLMIFSFSAMGQEIKDVPESHWAYDSVKLLV
                     DKGYLSLYEDGTFKGTNKVSRYELAEIIARMLEDTASGETEMSEEDVDKLRELSLDFR
                     DELVDIANKQNVFSENLDKVQKKNVIQDESIGKANERISEVQEEVSQIIDEIVTIKNL
                     NEKVNTLSTRVENLESELNTTNQKVAEKDQMISELRTELQNTDIQELRDKNSALESKV
                     NNLQGRLQELQQDVNNNNEQLTQLTSPEEEESQGGFQGSNTTLYLGAAVLLALLLGT"
     gene            9559..10176
                     /locus_tag="VKN64_06785"
     CDS             9559..10176
                     /locus_tag="VKN64_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HMA59981.1"
                     /translation="MKSLLIKSTSILLVILIALMVNSIIFTDPVFVPKANAVDLNGDQ
                     LLTGLGIAFVLYVLFESTDNDNLGGEEIKTPDFEDEQNYDISINSINLDLLARAIYAE
                     ARGESFEGQVAVGAVIVNRMESPKFPNTIEKVLYQPNQFKCVENGRINQTPNQNAYKA
                     ARRAIEGDDPSQGALFFFNPETSENPAAFDRYTVTVRIGNHIFAK"
     gene            10380..11741
                     /locus_tag="VKN64_06790"
     CDS             10380..11741
                     /locus_tag="VKN64_06790"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HMA59982.1"
                     /translation="MFKRKKGLLVLTLFLTLTLALTAGAQAEVKNMIVLIGDGLGQGQ
                     IDLTRYMTEGKDAELELMKLPNVGIMMTSSTQGVPDSAAAGTAMATGHKTDNGMVGMS
                     PTGAELDSILDYAETLNKSTGIISTNKVTDATPAAFAVSAESRDDEGAIAKAILNNDV
                     EVVLGGGADEFTAETAGEDVVKMAKEMGYEHVTNETELANAEAPNNKLLGLFNPSYMN
                     YKLDRDNVNSNEPSLKEMTKKAVQVLSRDEDGFFLMSEGARIDHAAHAADIHGVVAET
                     LEFDETVEYAVEWAKKNGNTMVVVVADHETLGFSVTEPMNKEALMNIEVSPEYMAAQM
                     KEKKNEPGMTIESIKNVFAEYANIEMTDQEAREFNDSIINNEGNMYYTYMIGWEIGSV
                     IAKEYNAGVLTSDVRAKSSTGGHTLNSVPIFAYGPESGAFNDVLDNTEFYEEMFQAFK
                     PGN"
     gene            11896..12267
                     /locus_tag="VKN64_06795"
     CDS             11896..12267
                     /locus_tag="VKN64_06795"
                     /inference="COORDINATES: protein motif:HMM:NF021463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HMA59983.1"
                     /translation="MKKETYQTIGKLFPILVGISVVLGIILDNTIIPLIVVGIGVPLI
                     IWSKKNVKEVIEDERDELIGGKAMRNAVILFALIATVIGLILYTQGGEVKMAVSHTLL
                     GSVSFILVSYIGFFAYLERKM"
     gene            12277..12474
                     /locus_tag="VKN64_06800"
     CDS             12277..12474
                     /locus_tag="VKN64_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA59984.1"
                     /translation="MKNELKVFRAKHDLTQQDLAEKVDVTRQTIISIEKGRYEPSLSL
                     AFKLANYFEVKIEEIFSYEED"
     gene            complement(12572..14974)
                     /gene="gyrA"
                     /locus_tag="VKN64_06805"
     CDS             complement(12572..14974)
                     /gene="gyrA"
                     /locus_tag="VKN64_06805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMA59985.1"
                     /translation="MTERISSIAIEEKMKEAYLNYSLSVIVSRALPDVRDGLKPVHRR
                     ILYAAEELGLTHDKDHKKSARIVGEVLGKYHPHGDQTVYNAMVRMAQDFNQRYTLIDG
                     HGNFGSVDGDSPAAMRYTEAKLTEMSEYILKDIKKETVDFSDNFDGTLQEPDVVPSRV
                     PNLLINGSSGIAVGMSTDIPPHNLNEVADAIMALIDDPNLTADDIIDNYISGPDFPTG
                     GTIVGNEGIKEAYKKGKGKLNVRGKHKVERVGRHKQLVITEIPYQVNKSRLIEEIAKY
                     ISKGKLDEIRDLRDETDKEGLRIVIELKQGADVDIILNRLYKFTSLQNSYRINLLALR
                     DNKPEVMTLLDILKYFIKFRREVITKRSEYELDQLQSRLHLLKGLTKAIDKLDLVISI
                     IRNSESAKEAHQKLKKELDISDEQAKAILNMQLRRLVGMEIEKIEAEVSELKGDIKEL
                     KKILNSKSKMNEVIIEELKEVKDKFGDERRTDILSDESKATITKQDLIKEKEAVISYS
                     LRQNIKRNTDITNMRASKNDYILELMKGSSYDKLLFFTASGQVYTLSIHDIEEHHGLS
                     TGEPLSKYVKIPMNEKIVSVLCLNDQLKEEYIDILTKNGKVKRTLGKEYDSSYTSINA
                     IKLDEDDEVIDIIESKGGNDLIIGTDNGKTIRFVEDSISSTGRYTMGSAGIKLDDNEA
                     VFMDKVDDHDYLVSISVDGKANKINVDNFHNQNRNGKGRNTSKSGNYEMHTMLLADKD
                     DTILIVTEENKMIELDVADISETQLPGYMYKIKEYSPEDKIKDIYKLPKIDTELEDEE
                     EK"
     gene            15188..17095
                     /gene="gyrB"
                     /locus_tag="VKN64_06810"
     CDS             15188..17095
                     /gene="gyrB"
                     /locus_tag="VKN64_06810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMA59986.1"
                     /translation="MDLFDNDRYNADKIQVLEGLEAVRKRPGMYIGSTGIKGLHHLVW
                     EVVDNSIDEFLAGFGNKIKVTVHEDNRVSVEDWGRGIPADLHKDTGRPAVEIVLTTLH
                     AGGKFNNNSYKVAGGLHGVGVSVVNALSEELKIETHWEGHKYTQTYQSGLPQNKLTKD
                     GKTDKSGTKITFKPDSKIFDTTKYKFETLAHRFKQSAYLNKDLTIILEDKRNDKKEEF
                     QYDGGIKAFIEYLNKNRNLLYDEIIYNQDSIEEYELEFAIQYNQGYSERIFSYANNIN
                     TVDGGYHLTGFKSALTKAVNNFAKKNNLLPKNVNGLNGHDVREGITAVINVKLPNPQF
                     EGQTKSKLGNSGLRSIVEKSAYDYLSLYLDTNPELGKAIVNKALKSVRARQASKKARE
                     LTKRKSVLSSSSLPGKLADCASKKADGTEIFIVEGDSAGGSAKQARNRHKQAILPLKG
                     KILNVERARLDKILNNTEIASIITALGTGVGEDFDLDNLRYEKIIIMTDADIDGAHIS
                     TLILTLFYRYMQDLIEEGHVYLARPPLYKVTYRNNSQYLFNEESLEEYRDEVGAGNFS
                     IQRYKGLGEMNPDQLWDTTMSPESRKLQKVEINDEIEADDVFTKLMGSNSKLRKKFIF
                     KNADLATEIDI"
     gene            17174..17683
                     /locus_tag="VKN64_06815"
     CDS             17174..17683
                     /locus_tag="VKN64_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HMA59987.1"
                     /translation="MNIISLIFGKGWPQRIVILTAIGLYIYLGLVKPEAAKAGISGAV
                     QTFIGMFTLIFAALLIANAISILIPTELITNYLGEESGIRGIVGGGLLAGFLQGGPYA
                     VYPIIKSLYDKGAHISVVISMLMGYGAIGIGRVAYGLAFFDPKIVGLRVLLALPVPII
                     AGLIVYYLL"
     gene            17750..18088
                     /locus_tag="VKN64_06820"
     CDS             17750..18088
                     /locus_tag="VKN64_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59988.1"
                     /translation="MKNYIWIPRILAIIFIIFISLFALDSFGTDAPLTQEIIGFLIHL
                     IPTFFLVIILYIFWKKPLYCGLSYIVLAVFFTIYFNTYRNIYSLILVSLLPAFIGFLF
                     IIFRKPLKRA"
     gene            18103..18567
                     /locus_tag="VKN64_06825"
     CDS             18103..18567
                     /locus_tag="VKN64_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA59989.1"
                     /translation="MLFRKPKKSDISYLIKLSKKFKQEYDWTDVVPIANIDSKEKAKK
                     RLFADNIKHIVIAEENDKMVGYLAVKRYEVDGELGHEASIIIHTDYRNQGLAKKMTEK
                     LFQEIPPDIEVEAWVLKDNIPSIKTVKSLGFEFDRNFAGDKRVQIYTKGGEK"
     gene            18763..>19017
                     /locus_tag="VKN64_06830"
     CDS             18763..>19017
                     /locus_tag="VKN64_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59990.1"
                     /translation="MNEEDKYKKNYIYLTLEGILFDVGFVFFDPTTILPLLLERLTGS
                     AFLVGLLTMVRYLGAGIFSLLAGNWNRTLKFKKKYLIKYSA"
BASE COUNT         6719 a         3030 c         3650 g         5618 t
ORIGIN      
        1 attaccttta gatatagcct taggggtagg cggaattccc aaaggcagaa ttattgaact
       61 atatggtaat gaatcttcag gtaaaactac cctgggacta catattattg ctgaagccca
      121 aaaacaggga ggtattgccg cttttatcga tgcagaacac gctctagatc cccgctatgc
      181 tgaaaatcta ggcgtaaata tagacgatct attaatttct caacccaata acggagagga
      241 agctttagaa atagccgagg ctttagttag aagtaatgct attgatttag tagttatcga
      301 ctctgtagca gccttagttc ctaaagcaga actggaaggg gaaatgggtg atacccatgt
      361 tggattacag gcaagattaa tgtctcaggc tatgagaaag ctttcaggtg ctatttcaaa
      421 gtccgatacc gcctgtattt ttatcaacca gattcgtgaa aaggtaggag tcatgttcgg
      481 aaatcccgaa acaacccccg gtggacgagc ccttaaattt tactcttctg tccggattga
      541 actgagaaga tctcaaacca tcaaagatgg tgatgagatc atcggttcac atgcgagggc
      601 taaggtagtc aaaaataagg ttgctgcccc ctttagaaag gccgagtttg atatcatgta
      661 tggaactggt atttctagaa cgggctgtgt actggatatg ggagaagata ctggcatcat
      721 agatagagct ggttcctggt actcctatgg tgatgtaaga ttaggccagg gcagagaaaa
      781 ttcaaaagaa ttccttaaag aaaatgaaga tatactaaaa gaaattgaac ataagataaa
      841 agttaaagtg ggcttaattg aagatgaaga aatagaggaa gaagaaacta aagaggaaaa
      901 agaggcctaa aactttatat attaatgatt gttaaaacct gtatattaac gtatacaggt
      961 ttttactttc ttgacacaaa ggtaaaaaaa agataaaata aatatgatgt atttgataat
     1021 tgaatatgtt tttaaatcaa actaggaggt gaaaataatt tataataccc tattaatagt
     1081 agccatatcc cttgtcccgg gattaattat tgggtacttt attagaaagt ttattgctga
     1141 agcccggatt cagtcggctg aagaagagtc tcgcaagatt ctcgaagagg cagaacgtga
     1201 ggcagaatct aaaattaggg aaattaccct tgaagccaag gagaaagccc aaaaaattag
     1261 agaagaagcc aaccgtgaaa gtcagaaaag aagaaatgaa cttcagcgac tggaagaccg
     1321 tttaatgcat agagaagaaa cacttgataa aaaaggcgaa acactcgaga aaaaagaaca
     1381 aaaacttaaa aaacaacaaa aagaattaga agaagaacaa caagaaatcg aagatatcaa
     1441 acaacaagaa atcgaaaaat tagagaaaat ttctaactta tctcatagtg aagcaaaaga
     1501 atatttactt tctaaaatgg aagatgaact cgaccatgaa tatgctaaga tgattaaaga
     1561 aaaagaaaga gaagccaaag aagaggctaa taagaaagcc cgtgaaatta tttctctggc
     1621 catccaaaga tgtgccggcg attatgtttc agaaacaact gtaacagttg tagaccttcc
     1681 taatgatgaa atgaaaggaa gaatcatcgg tagagaaggt agaaatatca gggctttaga
     1741 aagtcttaca ggcattgact taattattga tgatactcca gaagcggttg ttatctctgg
     1801 atatgatcca atcaagcgag aaatagcccg tatagcacta gaaaaactaa tcgttgatgg
     1861 tagaattcac cccgcacgta tcgaagaaat ggtggaaaaa gccgaagatg aattggacaa
     1921 tcaaatcaaa gaggttggtg aacaggccac ctttgattct ggtgtacacg gacttgattc
     1981 tcaattagtt aaatacctgg gacgattaaa atacagaact agttacggcc agaatgtatt
     2041 acagcactcc ctagaagttt ccttcttagc aggagtgatg gccgatgagc tgggagccga
     2101 ttcaacctta gctaaaagag gcggcttact tcacgatata ggtaaagcca ttgatcatga
     2161 agtagaagga cctcatattg atatcggggt caacttagcc cgtaaatttg gtgaatcgga
     2221 agatgtaatt cacaccattg aagcacacca tggtgatgta gacttccaat ctgtagaagc
     2281 tgtcttagta gcagcagcag atgctatctc agcagctaga cctggtgcta gaaaagaaac
     2341 attagaagca tatattaaga gattggaaga attagaagaa atcggacaat cttttgatgg
     2401 agttgaaaat gcttacgcaa ttcaagctgg ccgtgaaatt agagtcattg ttcaatctga
     2461 gcaagtagat gatacaacag ccagcaaact ttcgagagat attaaaaaag agatcgaaga
     2521 taaccttaat tatccgggac aaattaaaat tgttgtgatt agagagacca gacaaattga
     2581 atatgctaaa taaataagaa aataaaggcc cttttttaag ggccttttta aaatcataat
     2641 ggagtatgat caaaatgaat atactgttta taggagatat agtcggcagg gcaggtagac
     2701 gatcgattag aaagttaatg gatgatatag ttaaaaatca agatatagat tttgttttgg
     2761 ccaatggtga aaatgcagct ggtggttttg gcctaacaga agatactgca gaagaacttt
     2821 acagttatgg tattgattgt atgacaatgg gtaaccatac ctggggtaat aaaaatattt
     2881 acgaatttat cgatgataat gaaaaactta ttagaccctt aaactttaat gaaagggttc
     2941 cgggtagagg ttataatact tatcaaataa aggataaaaa cattactgtc ataaacctaa
     3001 ttggacaagt atttatgggt aaccataatt caccagtaga agcttttgat agagtttatg
     3061 agaaaatcaa agataaatct gattatatta tagtggactt ccatggggaa gccaccggtg
     3121 aaaaaagggc ctttgctgaa tatgtaaaag gtaaagttac tttggtagta ggcacacata
     3181 ctcatgtaca gaccaatgat gaacagattt tggataaagg aacaggttat atcacagacc
     3241 tgggtttctc aggtgcctgt gattctatct tgggaatgaa aaaagagaaa gtcctatata
     3301 gattgaaaac caaaatgccc aaaagacttg aagtggcaac gggtcaggtc cagttatcag
     3361 gtatttttgc tacttttaat caaaaaggtg aaacaaccgc aattaactcc tttattgtaa
     3421 gggataatcc ttaaaaaata tttttataat cttgcaggaa aatcctgctt ttataagaat
     3481 ataataaata caataaattt aatactaaat gagggggtat tgaagaagtg gatgtattaa
     3541 aagtctcagc agattctgat cctaattctg tagcaggagc attagctggt gtactaagag
     3601 aacaaggaag tgcagaactt caagcaatag gagctggtgc attaaatcaa ggagtaaaag
     3661 cagttgcaat agcaagagga tatgttgcac ccagtggtat tgatcttatt tgtattcccg
     3721 ctttcactga tattgagatt gatggtgaag aaaggaccgc tattaaatta attatcgaac
     3781 caagatagaa acggacaata acctgtttgt cttatctggc aaacaggttt ttttatgcaa
     3841 taaaattata attttagggg acttgaactt tggaggtaaa aaatgtttgt agatctacac
     3901 ttacacacca catattcgga cggatcctta acacctgaaa aattaataaa aagagcaaaa
     3961 gagatcggtt attctgccat agcagtaact gaccatgata ctttaaaggg ggtcaaaccg
     4021 gctttaaaca aagccgaaaa gaacaatata gaagttgtcc ctgggattga attcaatact
     4081 tccttaaatg gttatgatgt acatatctta ggttactata ttgatctaga aaacaaatat
     4141 ctcaaaaatt tattagataa aattaaaaaa gaacgtagag aaagaatcga aaaaatggta
     4201 gaattattaa agggacttta tggttatgag atctcactcc aagaaataaa aaatattagt
     4261 gaaaataata taatggggag ggcccatata gctcgccttt taactgaaaa aggctatgta
     4321 caagattgga aggaagtatt taataaatat attgggagag gtagacctgc ttatgtagat
     4381 agaaataaaa taaccccttt taaagcagtt gatattataa aaaaagcaga tggaattccg
     4441 gtaattgccc atcccggctt aattaaaaat aatcagatcg tccaacagat tatcaactat
     4501 ggggtagcag gcctagaagt ttattatctt gaacacagtg cagaacagat tgaatattat
     4561 aaagaacttg ctcaaagtaa tgaactttta ataacaggcg gctctgactg tcacggacct
     4621 aaaaacaagg acggcttaaa gctcggcaag attcgcttgg attataagta tcttgcagag
     4681 ttaaaaaaat acaaaacaat tgtaaatgat aaggcttata agaaggattt attatcttaa
     4741 tatagaatta gtttaattag gattaataat attagattat taaatattta taggacaagg
     4801 agtgtcaatt aatgtataat caaatctata attttatatc acttggggga ccaatggcta
     4861 tcccattaat tatctgttca atcttcagtg tagcagtaat tattgaaaaa gccgtatttt
     4921 tcatcagaag aaaagacaac agtttaagat tacttaaaag aatagagtta aaggttgaaa
     4981 atgatgatat tattggagct ttaaacgaac tcaagggaga aaaagggccg aatgcaaagt
     5041 tgctatctac agccctttct cattatgaag aagatcctga taggataaga gaagttctac
     5101 aggcggtagg agaagatgaa atcaaaaaga tggaaaaaca tttacctgta cttgatgtag
     5161 ttgccatgat ttcacccctc ttaggtctat tgggaacagt actcggcatc attagtagtt
     5221 tcaatatttt aggtactgct gctggtgcag ctaatcccgc tcagattagt tctggtattg
     5281 cagcggcttt aattagtact gcgatgggat tagttatagc tataccaaca gctatctttt
     5341 attcctacta tatcaataaa gttgaggaga aatctcatga gatgaacctc agcatgattg
     5401 atatcatgga tgttatcaca gacaggagtg aaagagatgt tcaaacctac acataaaaag
     5461 aaaacaaaca ttaacattat tccgatgata gatgttatat ttttcttatt agtgtttttt
     5521 atgttgttta catcctttcg caccaatccc tatggaatgg atatgaagct gcctaaagca
     5581 gtaactgtta ccgaacaaaa acaggaaaat ttcataatca atattaatga aaacggtgaa
     5641 tttttctatc agggagataa tcttcccatg tctaaaatta gtgaattagc aaaggaaaag
     5701 tatcaaaaca accaaaatct agttgttata atcaatgcag atagcaatac gaagtatagt
     5761 tcaattgtga ctgtaatgga tagtgtaaga caagtaggaa tctataatct tgccttagca
     5821 gctgaaaaga aaggtagtta ggtggtgtaa aatatgttta acaatgacag gagcaaatta
     5881 ttcctgtatg taactttatc attagtaatc caccttctcg tcttttattt tttcccattt
     5941 ggatatttac agggattcag tagtgaagaa gcttctggtg ataacttcgg ctatgtacaa
     6001 tatgtagaat ttaggtctca aaccaatcaa agagtagaac cacccgaaga tcctgaacca
     6061 caaccggctg aagaaccaga agaagaacca gaggaagagg aagaggaaga agaaccagaa
     6121 ccagaacctg agcccgaacc agagccagag ccggaaccgg agcctgaacc tgaaccagaa
     6181 ccggaagaac aaactactga ttctgatgtt gaaatagcac aagaaaatga accagaagaa
     6241 gaggtggaag aaactcaaac taaccaagat caggaagtaa taagtagtga agaaagcgat
     6301 actgaaatag aagtagagaa aaaagaagag gctaatcaag aacagcaaga acaagagcca
     6361 gaacccgaac ctgaaccaga acctgaacct gagccaactc ctccgcctcc accaaccgcg
     6421 ggagaactaa tcggtttatc accgaaacct gtttatccca agtatttagt tagtgaggct
     6481 aaaagtggaa aagtaggctt aagtgtaaag gtcgatcaaa atggtgaagt tgaaaatata
     6541 atggttacta actccagtaa tacagaaagc atggatcgaa atgccaaatt aaccgttgaa
     6601 aacggctggc aatttaaaag ttataaacaa agatatcaaa ttaatgtaac agttaatttc
     6661 aatattgatg aaaatggaaa cccgaacgta gatgtagaaa taggcaacgt aaactttctc
     6721 taaccaagca cgggaggttt aatcattgaa aaatagacaa agattaatag taacaattat
     6781 attacttgtc tcatttttat ttatttctgt cttaacatcg gctcagtccg atgtttttac
     6841 agaagtaggt agtaataata atttgagatt aggtaatgat tatatagtaa tatttataaa
     6901 ccaagatgat aatgccaaag gtcgttttgc tatcgaaact accggaggtg ctccgatgga
     6961 accagctgat gataataaac ctctggtata tggtagacct aaaccctgga cttcttatac
     7021 tactttaaag atcaataatg taaattatgt ctttggcggc tctactaaaa gaagagccgg
     7081 tgatgatgct aattatggag aagttaccga aggtcctgta attaaagata atcaaattta
     7141 tactagtact ttaattgatg gaataaaagt agaacaaatc ctatctatag taaaaagtag
     7201 taccaccggt ttatttgata cagctcaaat taagtataga ctaatcaaca acagcaatca
     7261 ggaaaaagaa gttggattaa ggattatgct ggacaccatg ttaggtcaaa atgacggtgc
     7321 tccttttagg ttagcagata aagctgttac cacagatacg ctctatcttc aaggagaact
     7381 accaagattt tggcaggcgt ttgacagtat cacgaaccct accgtaactt cccagggtac
     7441 ctttagagga cccggagtta ctgtccccga taaagtctac tttgctgatt ggggcagctt
     7501 agccgatgga gactggaatt ttgactttaa tcccggcgaa gaatttgtac ggaaaggtga
     7561 atatgaaatt gacagtgcga ttgcgataat gtgggaacca aaactactgg cacccaatga
     7621 agaagtcacc tatgtaacta actatggtct gggtggaatt aaagtcgtac ctggattatt
     7681 agcactaggt gtaacttcac cggctgaatt tacatttgat gaaaataatc aatcattccc
     7741 ggtagtagca tatgtagaaa acacatcaga aattacagct gaaaatgtta aagtagaaat
     7801 agatctgcca cagcagttta ctgctgctaa ccccgttaaa gaaattggtg acctaaagtc
     7861 caaagatact gcccaggtaa gctggcaggt aagacccgcc ggagaagaaa ttccaggcaa
     7921 catgacttat agagtcaaag tatctgccga caataccgat tctaacgagg tagaaagaga
     7981 aatccgcttt gtcggaccac cagctattga agcagaagtt accttggaag aaaatctaag
     8041 aaaagaaaag ggtgaaatta aacctaatcc atttgatatg agtgtcaaat tcaccaatat
     8101 aggtggttca acattatttg atgcccaaac tgaacttgct ttaccacccg ggttaacctt
     8161 agcagataaa gaagtaaaca gaaaatactt aggttacttg gaaagtggtg aaagtatcga
     8221 tgttaattgg tctgtaaggg tactcaatgt aacgggagaa atgccttact ccattaatat
     8281 taccggttta aataactata acaagacaaa aagagaagat ttagatatac cagaattaga
     8341 accattcgtc tatttagaaa aagttgagtt tggtgaaagt tatcaaactt acaacttaat
     8401 aggacaaaat ctagccactg ttaatagttt aaaacttaat ctaagttata atccagccgc
     8461 ttacaggatt aattatacat tcccgggtga tatgtttgtc caaaatgata agattatcct
     8521 ttgggaagat cctgtaattt cagacgggag aattgaaatg aatgaaattc tgcccgataa
     8581 tgctgaatct ggcgttatcg ccaccttaca gtttagtaag aaaagtgaaa acccactcaa
     8641 tttaacaata gatagtatag aggccatgga tgagaaaaat aatgaagtcg atattaaata
     8701 tgaaaactta aaggaggaat agttttaaga tgaaaagatt tcgtaattat ttagtcttta
     8761 ccttaatatt tttaatgata ttttctttct cagcaatggg acaggaaatt aaagacgtac
     8821 cagaaagtca ctgggcatat gacagtgtaa agttactagt tgataaggga tatctctcac
     8881 tatatgaaga cggtaccttt aagggtacaa ataaggtaag cagatatgaa ttagctgaaa
     8941 ttattgcccg tatgttagaa gatactgctt caggtgaaac agaaatgagt gaagaagatg
     9001 ttgacaaatt aagagaatta tctcttgatt ttagagatga actggtagac atagccaata
     9061 aacaaaatgt cttttcagaa aatctcgata aggtacaaaa gaaaaatgta attcaagatg
     9121 agtcgatcgg taaagcaaat gaaagaattt cggaagtaca ggaagaagta agccaaatta
     9181 tagatgaaat tgtgaccata aaaaatctaa atgaaaaggt caatacctta tctacaagag
     9241 tagagaatct ggaaagcgaa ttaaatacta ccaatcaaaa ggtagctgaa aaagatcaaa
     9301 tgatatcaga attaaggact gaactacaaa ataccgatat tcaggaatta agagataaaa
     9361 attctgcttt agaatcaaaa gttaacaatt tacagggcag actacaagaa ctacaacagg
     9421 atgttaataa taataatgaa cagcttactc aactaactag tccagaagaa gaggaaagcc
     9481 aaggtggatt tcagggaagt aatacaactt tatatttagg tgcagcagta ctattagcac
     9541 tattattagg tacctaatat gaaaagtctg ttaattaaat ctacttcaat attacttgtt
     9601 attttgatcg ccctaatggt caactcaata atttttacag accctgtttt tgttccaaaa
     9661 gcgaatgctg ttgacctgaa tggagatcaa cttctgactg gactgggaat tgcctttgta
     9721 ctttacgttt tatttgaaag tactgataat gataatttgg gaggagaaga aataaaaact
     9781 cctgattttg aagatgaaca gaattatgat atcagtatta actctataaa tttggacctt
     9841 ttagcccggg ctatatatgc cgaagcaaga ggtgaatcgt ttgaaggcca agttgctgta
     9901 ggcgccgtta tagtcaatag gatggaaagc cctaaatttc ctaatacaat agaaaaagta
     9961 ctatatcaac caaaccaatt taaatgtgtg gaaaatggaa ggattaatca aactcctaat
    10021 caaaatgcct ataaagcagc ccgacgggct attgaaggag atgacccctc tcagggagcc
    10081 ttgtttttct ttaatcctga aacctctgaa aatccagctg cttttgaccg ttacacagta
    10141 acagtaagaa ttggcaacca catatttgca aaataatcaa attaaatgaa atcataaagc
    10201 atgtaaacct caatcagttt acatgctttt tttattttct ttttgttaat tttttatgaa
    10261 aatcacttct cttatttaaa taattttact aaaaggaaaa tatatatttt tatttaaaat
    10321 attttattag gaaaggatta aataataaat taatatttta ggggggtaac aaattaatta
    10381 tgtttaaacg caaaaaggga ttattagtgc taacattatt cttaacattg acacttgctt
    10441 taacagcagg ggcccaagct gaagtaaaaa atatgatcgt actaatcggt gatggtctag
    10501 gacagggtca aattgatctg actagataca tgaccgaggg gaaagatgct gaattagaac
    10561 tgatgaaact cccaaatgtt ggtataatga tgaccagttc cactcaagga gtacctgatt
    10621 cagctgcggc cggtacagcc atggcaaccg gtcataaaac tgacaatggc atggttggca
    10681 tgtcaccaac tggcgctgaa ttagattcca tccttgacta tgcagaaact cttaataagt
    10741 ccactggtat catcagcacc aacaaagtaa ctgatgctac accagctgct tttgcagtat
    10801 ctgctgaaag cagagatgat gaaggcgcaa ttgccaaagc tattttaaat aatgatgtag
    10861 aagtggtttt aggcggtgga gctgatgagt ttactgctga aactgctggt gaagatgttg
    10921 taaagatggc taaagagatg ggttatgaac atgtaaccaa tgaaactgaa ttggccaatg
    10981 cagaagctcc taataataaa ctattaggtt tatttaatcc ttcctatatg aactataagc
    11041 ttgaccgtga taatgttaat tcaaatgaac cttctttaaa agaaatgacc aaaaaagcag
    11101 ttcaagttct ctcaagagat gaagatggtt tcttcttaat gagtgagggc gcacgtatag
    11161 accacgctgc tcacgctgcc gatatccatg gtgttgttgc tgaaacctta gaatttgatg
    11221 aaaccgttga atatgctgtt gaatgggcta agaaaaatgg caatacaatg gttgttgtag
    11281 tagcagatca tgaaacatta ggcttctcag taacagaacc gatgaacaaa gaagctctca
    11341 tgaatattga agtttcacct gaatatatgg ccgctcaaat gaaagaaaag aaaaatgaac
    11401 ccggcatgac tattgaaagt attaaaaatg tatttgcaga atatgcaaat attgaaatga
    11461 ctgatcaaga agcaagagaa tttaacgatt caataataaa taatgaaggt aatatgtatt
    11521 atacctatat gatcggttgg gagattggtt ccgtaatagc taaagaatac aatgctggtg
    11581 tattaacctc tgatgtaaga gcaaaatctt ccactggtgg ccatacactt aattctgtac
    11641 caatttttgc ctatggacca gaatctggcg catttaatga tgtgttagat aacactgaat
    11701 tctatgaaga aatgtttcaa gcatttaaac ctggtaatta attaaaataa agaatatcaa
    11761 aagggggagt gcaaactccc tctttttttt attataatat tgtaaagttt acttgacatt
    11821 ttgtaagtat tgttttacaa tatatattgt aaagtaagct ttacataatt atatataaag
    11881 agagtgtgag ataaaatgaa aaaagaaaca tatcaaacta ttggaaagtt atttcccatc
    11941 ttggtaggta tatcagttgt attgggaatt attttggaca atactataat acctttaatt
    12001 gtggtaggga taggagtacc tttaataatc tggtcaaaaa agaatgtaaa agaggtaata
    12061 gaagacgaaa gagatgaatt aatcggtggt aaagcaatgc gaaatgctgt aatattgttt
    12121 gctttaattg ccacagttat aggcctaata ttgtatacac agggtggaga agtaaagatg
    12181 gcggtttctc ataccttatt aggtagtgtt tcttttatcc tggtaagcta tattggattt
    12241 tttgcctatc tggaaagaaa aatgtaggtg aaatatatga aaaatgaatt aaaggttttt
    12301 agagcaaaac atgatctaac ccaacaggat ctagctgaaa aagtggatgt aacccgccag
    12361 accataatct cgatagaaaa gggaaggtat gaaccgtctt tatcattggc cttcaaatta
    12421 gctaattact tcgaagtaaa aatagaggag attttttctt atgaagagga ttaattaatc
    12481 tctgataaaa taaggaaagg aatgagtaaa aaagcaccca ttccttttaa tcttctaata
    12541 ctatatctta tttttttatt tttttgtatc attatttttc ttcttcatct tcaagttcag
    12601 tatctatttt gggcagttta tatatatctt tgattttatc ttctgggctg tattctttaa
    12661 tcttatacat gtaacccggc agctgtgttt cagaaatatc ggcaacatct aattcgatca
    12721 tcttgttttc ttcagtgaca attaaaattg tatcatcttt gtcagctaag agcatagtat
    12781 gcatttcata attaccggat ttggaagtat tccggccttt accattccta ttttgattat
    12841 ggaaattatc tacatttatc ttatttgcct taccgtccac tgaaatagag accaagtaat
    12901 cgtggtcatc taccttatcc ataaatactg cctcattatc atctagttta attccagcac
    12961 tacccatcgt atagcggccg gtactgctga ttgaatcttc aacaaatctt attgtcttac
    13021 cattgtcagt accaatgatt agatcattgc cacctttgct ttcaattatg tcgatcactt
    13081 cgtcatcttc atctaattta atagcattaa tagaagtata gctagagtca tactccttac
    13141 caagggtcct tttaacctta ccatttttgg ttaagatatc tatatattct tcttttagtt
    13201 gatcatttaa acataataca cttactattt tttcgttcat aggtatctta acatacttac
    13261 ttaatggttc accagttgaa agaccgtggt gttcttcaat atcatggatt gataaggtat
    13321 agacctggcc gctggcggta aagaaaagca gtttatcata ggaactacct ttcatcagtt
    13381 ctaatatata atcattttta gaagctctca tattcgtaat atcagtattt cttttaatat
    13441 tttggcgcag tgaataggag ataactgctt ctttttcctt gattaaatcc tgtttagtaa
    13501 ttgttgcttt actttcatcg gagaggatat cagttctacg ttcatctccg aacttatctt
    13561 taacttcttt taattcttca atgattacct cgttcatctt acttttactg ttcagtatct
    13621 tcttaagttc ttttatatca ccttttaatt cacttacttc cgcttcaatt ttttctattt
    13681 ccattcctac taaacgcctc agctgcatat ttaagattgc tttggcctgt tcatcagata
    13741 tatctaattc cttttttagt ttttgatgag cttccttagc agattcagaa tttctgataa
    13801 ttgaaattac taaatctaat ttatcaatcg cctttgttaa tcccttgagt aggtgtagtc
    13861 gagactgtaa ttgatctaat tcatattctg atctttttgt aattacttcc cgcctgaatt
    13921 ttataaaata ttttaagata tccaataagg tcataacttc cggcttgtta tcccgtaagg
    13981 caagcaggtt aattcggtag ctgttctgta aggaggtaaa tttatatagt ctgttaagga
    14041 taatatcaac gtctgcgccc tgtttgagct caataacaat ccgtaggcct tctttatcag
    14101 tttcgtctct taaatccctt atttcgtcca atttaccctt actaatatat ttagcaattt
    14161 cctcaataag tctcgattta tttacctgat agggtatttc tgtgattacc aactgtttat
    14221 gtcgtcccac acgttctact ttatgttttc cccttacgtt aagcttgcct ttaccttttt
    14281 tataggcttc cttgattcct tcattgccaa ctatagtgcc gcctgttgga aagtcgggtc
    14341 cagaaatgta attatcaata atatcatcgg ctgttaagtt ggggtcatca attaaggcca
    14401 taatagcatc ggctacttcg tttaaattat ggggtggaat atcagtactc ataccgacgg
    14461 caataccgct tgaaccattt attagaaggt taggaacccg ggaaggaact acatctggtt
    14521 cttgtaaagt accatcaaag ttatcactaa aatcaacggt ttctttttta atatctttta
    14581 agatgtattc tgacatctca gtcagtttgg cctctgtata acgcatcgcg gctggtgaat
    14641 cgccatcaac cgatccgaag ttaccatgac catctatcaa agtatatctc tggttaaagt
    14701 cctgggccat tctaaccatt gcattataga cggtctgatc tccgtgggga tggtatttac
    14761 ccaatacttc accaactatc cgggctgatt ttttatggtc cttatcatga gttaaaccca
    14821 gttcttccgc tgcataaagg atccttctat ggaccggttt taaaccatcc cttacatctg
    14881 gtagtgccct tgatacaata acactcagtg aatagttaag ataagcttct ttcatctttt
    14941 cttcaatagc aattgaggaa attctctctg tcataatata cccctttcaa aaaaattaaa
    15001 taagtaatat gttataataa tatatgaata aaaaattaga acaaaatagt ttaaaattat
    15061 taatttaaaa acctttatcc tatttgatat tataccacgt agccatatta ttgcaagaat
    15121 gggtggcaaa tccataaact ctttgttata ataataatgg taactttaag aaaggatgga
    15181 tgtataagtg gacttatttg acaatgacag atataatgcg gataaaattc aagtattaga
    15241 agggctggag gccgttcgta aaaggcccgg catgtatatc ggctcaactg gtatcaaagg
    15301 acttcaccat ctagtatggg aagtcgtgga taacagtatc gatgaatttt tagccggatt
    15361 tggaaataaa ataaaggtta cagtacatga agataacagg gtcagtgtag aagactgggg
    15421 aagaggtatc ccggctgatt tacataaaga caccggccgc cctgccgttg agatagtgct
    15481 tacgacctta catgcaggtg gtaagtttaa caataatagt tataaagtgg cgggcggatt
    15541 acatggggta ggtgtatcgg ttgtaaatgc cctctccgaa gaattaaaga tagaaactca
    15601 ttgggaaggc cataaataca ctcaaaccta tcaaagtggt ttaccccaaa ataaattaac
    15661 aaaagacggc aagaccgata aatcaggtac aaagatcacc tttaaaccgg acagtaaaat
    15721 ctttgataca acaaaatata aatttgaaac tttagcacac cgctttaaac agtctgccta
    15781 tttaaataaa gacttaacta tcattttaga agataaaaga aatgataaga aagaagaatt
    15841 ccaatatgac ggcggtatta aggccttcat agagtatcta aataagaata gaaatttatt
    15901 atatgatgaa attatatata atcaagactc aattgaagaa tatgaattag aatttgccat
    15961 tcaatataat cagggctatt ccgaaagaat tttttcctat gccaataaca taaatacggt
    16021 agatggaggt taccatctaa ccggcttcaa gtcggcttta accaaagcgg ttaataactt
    16081 tgccaaaaag aacaacctct tacctaaaaa tgtcaatggt ttgaacggtc atgatgtaag
    16141 agaggggata acagctgtca ttaacgttaa actgcccaat ccccaattcg aaggccagac
    16201 taaatccaaa ctgggtaata gtggattaag aagtatcgta gaaaaaagtg cttatgacta
    16261 tctaagcctt tatttagata ccaatcccga actgggtaaa gcaattgtaa ataaagcttt
    16321 aaaatcagtc agggcccgtc aggcttccaa aaaggcccgt gaactcacga aaagaaagtc
    16381 ggtactatct tcaagttcct tacctggtaa attagcggac tgtgccagca aaaaggcaga
    16441 tgggaccgaa atatttatcg tagaaggtga ttcagccggt ggttctgcta aacaggctag
    16501 aaaccgacat aagcaggcta tattaccact caagggtaaa atacttaatg tagaaagggc
    16561 ccgccttgat aagattttaa acaatactga gatagcttct ataatcacgg ccttaggtac
    16621 aggagtcgga gaagactttg atttagataa tctaaggtat gaaaaaatta ttatcatgac
    16681 agatgctgat atagatggag cccatatttc caccttgatc ttaaccctat tttatagata
    16741 tatgcaggat ttgattgaag aggggcatgt ctatttagcc cgtccacctc tatataaagt
    16801 aacctatcgt aataacagtc agtacttatt caatgaagaa agtttagaag aatatagaga
    16861 tgaggtggga gcaggtaatt tcagtattca aaggtataag gggttgggag aaatgaatcc
    16921 cgatcaactc tgggatacaa ccatgtctcc cgaatcaaga aaattacaaa aagtagagat
    16981 caatgatgaa attgaagcag atgatgtatt caccaagtta atgggttcta attcaaaatt
    17041 aagaaaaaaa ttcatcttta aaaatgctga ccttgcaact gaaatcgata tttaatttta
    17101 attcccacaa ttatcatatt gtgggttttt tattttaaaa taaattaaat atagaaaaac
    17161 aggggtgatt ttgatgaata ttatttcact tatctttggt aagggttggc ctcagaggat
    17221 cgtaatttta accgcaattg gattatatat ttatctgggt cttgttaaac cggaagctgc
    17281 caaagcagga attagtgggg ctgttcaaac ttttattggt atgttcacct taatatttgc
    17341 tgcccttttg attgcaaatg ctatctctat cttaatacct accgaattga tcacgaacta
    17401 tttaggggaa gaatccggga tcagaggtat tgtcgggggc ggcttattgg ccggtttttt
    17461 acagggtgga ccttatgcag tatatccaat tataaaatct ctctatgata agggtgccca
    17521 tataagtgta gtgatctcga tgttaatggg ctatggggca atcggtatcg gcagggttgc
    17581 ctatggactg gccttttttg atcccaaaat agtgggacta agagttctac tagctttacc
    17641 ggttccgatt attgccggac ttattgtata ttatttacta tagaatattt aaaaaaactt
    17701 aaaaaaggaa tataatatat ttaagaatag aagagagggg tagattataa tgaaaaacta
    17761 tatctggatc ccccgtatct tagcaataat ttttataata ttcatttctc tgtttgcact
    17821 cgactctttt ggaacagatg cacccttaac acaagaaata attggattct taatacacct
    17881 tatcccaaca ttttttttag taattattct ctatatattc tggaaaaagc cgttatactg
    17941 cggcttatcc tatatagtac tggcagtatt ttttactatc tatttcaata cttaccgaaa
    18001 tatctacagc ttaatattag tttctctctt accagctttt atcggctttc tgttcataat
    18061 ctttcgtaaa ccattaaaaa gagcataaag gagataaaaa atatgttatt tagaaaacct
    18121 aaaaagtctg atataagtta tttaattaaa ctttctaaaa aatttaaaca agaatatgac
    18181 tggaccgatg tagtccccat cgccaatatt gatagcaaag aaaaggcgaa aaaaagatta
    18241 tttgccgaca atatcaaaca catcgtaatc gcagaagaaa atgataagat ggtaggatat
    18301 ttagccgtaa aaagatatga agtagatggg gaattaggcc atgaagcttc aataatcatc
    18361 cataccgatt acagaaacca aggcctggct aaaaaaatga cagaaaagct ttttcaagaa
    18421 ataccacctg atattgaagt tgaagcctgg gtactaaaag ataatattcc atctataaaa
    18481 actgtaaaat cattgggctt tgaatttgat agaaactttg caggtgataa gagagtacaa
    18541 atatatacta aaggcggaga aaaataagct aattattcat aaaaagaaat atgaaatagt
    18601 tcttttagtt tataaaaaat atgattaatt gaaaaaatta tttgaaagaa attttctttt
    18661 atagataaaa ttatataaag aactcgatta aaagataaat cggatcgggc agtattatct
    18721 ccccttaaga tagtgctgcc cagcttttaa agaggtaatt gtatgaatga agaagataaa
    18781 tataaaaaaa attacatata cttaacctta gaaggtattt tattcgatgt aggatttgtg
    18841 tttttcgatc ccaccacaat attaccatta cttttagaaa gactaaccgg ttctgctttt
    18901 ttggttggtc ttttaactat ggtaaggtat ctgggggcag gcatattttc tttattggcc
    18961 ggaaattgga atcgaacttt aaagtttaaa aagaaatatc taattaaata cagtgca
//
LOCUS       DATGCJ010000135        10251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_107973, whole genome shotgun sequence.
ACCESSION   DATGCJ010000135 DATGCJ010000000
VERSION     DATGCJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107973"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VKN64_06835"
     CDS             <1..187
                     /locus_tag="VKN64_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HMA59991.1"
                     /translation="DLPGTRGETLTPLRPAGIVLINGERVDVVSDGGYIDKGKDVEIV
                     SVSGSRIVVRKIIKESD"
     gene            188..1183
                     /gene="floA"
                     /locus_tag="VKN64_06840"
     CDS             188..1183
                     /gene="floA"
                     /locus_tag="VKN64_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277600.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HMA59992.1"
                     /translation="MFLPILVIVGVVIFLGLFFYFIPLGLWISAVAAGVKVGFFNLIG
                     MRLRRVVPSLIVGPMIKSHKAGLKLGSDQLEAHYLAGGNVDRVVDALIAAQRAEIDLS
                     FERAAAIDLAGRDVLEAVQMSVNPKVIKTPVVTAVAMDGIQVMATARVTVRANIERLV
                     GGAGEETVLARVGEGIVTTVGSAETHKKVLENPDSISRTVLEKGLDSGTAFEILSIDI
                     ADVDVGKNIGAQLQTDQAEADKEIAQAKAEERRAMAVAKEQEMRAEVQEMRAKVVEAE
                     ADVPKAIAEAFKQGNLGVMDYMNYKNIEADTDMREGISEFTGEQEEDYDEDKEDK"
     gene            1201..1623
                     /locus_tag="VKN64_06845"
     CDS             1201..1623
                     /locus_tag="VKN64_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA59993.1"
                     /translation="MDNIFSYWPLLFLIFAIIGRIMRYLNKRKKQQERQTSENIDNIK
                     EVTKQGQKAEAEPYEQYDTFSYDEDDEFETITDEKTDEFLEREAEKTEDKGPKKPQIK
                     KLKSGKKPNLFNKKEDILRGIIMKEVLDQPRYKKKKNK"
     gene            1823..2431
                     /locus_tag="VKN64_06850"
     CDS             1823..2431
                     /locus_tag="VKN64_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA59994.1"
                     /translation="MELSKRQKEIIEIVKNQGPISSKKIADQLGLSRATIRSDLSILS
                     MLKILDAKPNVGYYLLQEDYQLREIERLYKRNVKEIMSDPIHVKEKTSIYDTIVKMFL
                     EDVGSIVVVNEEEKLSGVISRKDLLKMSIGQSNLKNTPVSLAMTRRPKIITVSPETTF
                     IEAARKIRYHKIDFLPVITENDEVIGRISKTSIIKAFVDYAE"
     gene            2837..3799
                     /locus_tag="VKN64_06855"
     CDS             2837..3799
                     /locus_tag="VKN64_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMA59995.1"
                     /translation="MVPVEEQIDINDYQVSRNVFGNKDSNIKIIEDKLNVKIVARGSN
                     VKIEGKKDDVDLTKRVIEEMIKLAKTGKNIDKRQVKYAVDLLSKNRHMDLSDLYDEVL
                     QVNFKGREIKVKTLGQKYYVEAIKSSDIVFSIGPAGTGKTYLAMVMAVKSLLNNNANR
                     IVLTRPAIEAGEQLGFLPGDLQDKVDPYLRPLYDALYDILGPEKVSEYLEKDIIEVAP
                     LAYMRGRTLDDSFVILDEAQNTSPEQMKMFLTRIGFNSHAVITGDITQIDLPRSSNSG
                     LIQVRKILKDIKGIEFVYLTNRDVVRHELVKDIIKAYERYESGN"
     gene            3802..5961
                     /locus_tag="VKN64_06860"
     CDS             3802..5961
                     /locus_tag="VKN64_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HMA59996.1"
                     /translation="MQFFERIKNRFDKWTDFDIKLNDIYKRRISLVIFFIVIALILTF
                     NFFPNQIDLEVGQVAKSDIVAPRTVTFIDQEKTEQLRELAVESASKVYEEDKSVNRRI
                     LNEIDNLFNTVVNTKTEMSSDSTGQINNQFLTEVKNNNDLNINNNDLNLLLKTNIDKL
                     NSLRSSANDIMQEVLQERIFPENIIEVRNKINQLALETNFEKEYRLVLVNILTNHVEP
                     NMILDLDATEQRQQEALRQVEPVKRTVLKGENIIRKGDIVSQDDIQVLEALGLRKPSI
                     NYVNLLGVLITLSVLVFLLGYYIKHYEKEVWENINKLILIETLIILVILLAKIISIFQ
                     PTYMVYLVPAAIASILITVLINTEIALVVTVFISFLTALVFSNLYIAAAAAFVGGLVG
                     IISVSNVDQRSDLIRAGFNVSVSLVVFIAGLTMMDLTNNWFELLWSASMGILNGIFVA
                     ILANGLLPYLENIFDLTSSVKLLELSNPSHPLLKRLLVEAPGTYHHSIIVGNLAENAA
                     DKIGADSLLTRVGAYFHDIGKLKRPYFFSDNQFGGENPHSKTSPNLSALIIKSHVKDG
                     LELADKYNLPKAVKDIIQQHQGTGLISFFYKQAIEKSKHGNINESDFRYDGPKPQSKE
                     AALIMLADTVEAAVRSKNFNKNNHNRIELFVKELIREKLNDGQLDESSLTLHELDEIA
                     ESFVQILTGIYHKRIEYPDNLIKEIKKDDKNDKNGNQ"
     gene            5939..6367
                     /gene="ybeY"
                     /locus_tag="VKN64_06865"
     CDS             5939..6367
                     /gene="ybeY"
                     /locus_tag="VKN64_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMA59997.1"
                     /translation="MIKMEINDLQDKVEIEEDLYEDFKKIITKATEMEGYDGGEISIA
                     FVNNEKIKELNNKFRNINEVTDVLSFPMDEEILGDIIISAERALSQSKDYGHSFKREI
                     CYLVTHGILHLLGYDHKTDGEKEEMRKKEERILKELEIKR"
     gene            6422..7672
                     /locus_tag="VKN64_06870"
     CDS             6422..7672
                     /locus_tag="VKN64_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMA59998.1"
                     /translation="MYINLIGFVFLLILSGFFSGSETAFMSANRVRIRELANEGDKQA
                     GRVDNLLEDQTKLLTTILIGNNLVNIAASAIATSIAINIFGSKGVGIATGFVTLVILV
                     FGEITPKSLGNNSPIAYAKKASIILYWLEKILYPVILFFTILIKKMIGENKMISSAFI
                     SEEEVRRFVNVSEEEGAIKESEKEMIQSVFEFDDIVVKEIMVPRIDMVCISKDASINE
                     LVDLAVEKGHSRIPVYEESIDDIIGLIYVKDLLQLLQKGKENLTLEDLIKPIYFIPES
                     KQINKLLKEMQNRREHMAVILDEYGGTSGLITIEDLLEEIVGDIQDEFDLEDKQINLI
                     NNKEILVDARVDLDDLNEILPTPLLDEESYETISGFVLHKLGYLPEEKETIELEGVTI
                     EVEKIDEHRIQKLRIYTVEPIKKI"
     gene            7747..8922
                     /locus_tag="VKN64_06875"
     CDS             7747..8922
                     /locus_tag="VKN64_06875"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HMA59999.1"
                     /translation="MKKGLLYGLVILLVIVAVSAVLLRGRLDEETPEMTEQQVTEEAP
                     SEEESSQTDQDSEEQEEQEVEKETEDEKEEESEEETEDDQTDEEQDAQEEDVILSKFN
                     HLPIEEKVLHRAVMVSVENTPKARPQSGLCQAPIVYEFLVEGGITRFLALFWSDIPGK
                     IGPIRSVRPYMIEIAQEYDALLLHAGASPDGFEMLDKIDYTNLDQIYNGKYYWRSQQR
                     PTPHNLYTGEYRINNYLEDMLGIEYENRFNFEEIMIISNAKAGAEEISIDYWGNYEVL
                     YEYNRENNYYKRFLNSKDNPHLCGDEEQITVKNIIVQFVQTEVKDDVGRLSMKLEGEN
                     KALYFRNGVVSEGHWQKNSSGLTKFYDENDQLITVNPGNTWIQIVPNNTNITYKESD"
     gene            8925..9329
                     /locus_tag="VKN64_06880"
     CDS             8925..9329
                     /locus_tag="VKN64_06880"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMA60000.1"
                     /translation="MIDKKTKNKLLKAAKEVKENAYVPYSNFPLAAALLTKEGKIFTG
                     VNIENAAYGVTMCAERTAIFKAVSEGYTEFEALLLLSNTEKPITPCGSCRQVISEFSN
                     DIDIIMTTYDNGELIKKNKELLPGSFNKDDMK"
     gene            9329..10213
                     /gene="era"
                     /locus_tag="VKN64_06885"
     CDS             9329..10213
                     /gene="era"
                     /locus_tag="VKN64_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMA60001.1"
                     /translation="MSFKSGFVTIIGRPNVGKSTLVNYLIGEKISITSPRPQTTRNSI
                     KAIYTEDAGQIIFVDTPGIYKTKNDLDKYMVNEAYKSLEGIDVIIFMLDATEKFQKGD
                     QIIYNQIKNSSNPFLIVLNKIDKVKKKELPNILKDYNKRIEENIIPISSKTGRNVNNL
                     INEIFELLPAGPQYYPDDMITDQIEQFIVAEMIREKTFYLLREEVPFGIAVVIEEMKE
                     RNDETMYIRANIYVEKKSHKGIIIGKNGKMLKKIGTRARKDVEKLLQQKVYLDLWVKI
                     EKDWRDDENLLRRMGYKG"
BASE COUNT         3832 a         1319 c         2093 g         3007 t
ORIGIN      
        1 tgacctgcct ggtactagag gggaaacatt gacacctttg cgtccggcag ggatagtgtt
       61 gataaatggt gaaagggttg atgtagtcag tgatggtggt tatatagata aaggtaaaga
      121 tgttgaaata gttagtgttt cgggaagcag gatcgtagta agaaaaatca taaaggagag
      181 tgactaaatg tttctaccaa tattagttat tgtaggtgta gtaatattcc tgggcctgtt
      241 cttttacttt attcctctgg ggctctggat ttcagctgtc gccgcaggag ttaaagttgg
      301 attttttaat ttgattggga tgagattaag gagagtagtt ccttcactta ttgttgggcc
      361 tatgattaaa tcacataaag ccggacttaa acttggtagt gatcaattgg aagcacatta
      421 tctggccggt ggaaatgtag atagagttgt agatgctttg attgcagctc agagagcgga
      481 gatagattta agttttgaac gggcagctgc aatagattta gccgggcgtg atgtgctgga
      541 agctgttcag atgagtgtta atcctaaggt tattaagaca cctgttgtta ctgctgtggc
      601 tatggatggt atacaggtaa tggcaactgc cagagtaaca gtcagggcca atattgaacg
      661 tcttgttggt ggagcaggtg aggaaactgt gctggcccgg gtaggtgaag gcatcgtaac
      721 tactgttggt tcggcagaga cacataaaaa ggttctggaa aatcctgatt ctatttcccg
      781 gactgttctg gaaaagggtt tagattcagg tactgctttt gaaattcttt caattgacat
      841 tgcagatgtt gatgttggta aaaatatcgg ggcccaatta cagactgacc aagctgaggc
      901 tgataaggaa atcgctcagg ctaaggctga agaaagaaga gcaatggcgg ttgctaaaga
      961 gcaggaaatg agagccgagg tacaagaaat gcgtgctaaa gttgtagaag ctgaagctga
     1021 tgtaccgaaa gcaattgcag aggcctttaa acagggtaat ctgggtgtta tggactatat
     1081 gaattacaaa aatattgaag ccgatactga tatgagagaa ggaatttccg aatttaccgg
     1141 agaacaagaa gaagattatg atgaagataa agaggataaa taaagtagag gtggattgaa
     1201 atggataaca tttttagtta ttggcccctc ttatttctca tatttgcgat cataggtagg
     1261 ataatgaggt atttaaataa gagaaaaaaa caacaggaaa gacagacctc ggagaatata
     1321 gataatataa aagaggttac aaagcaaggt cagaaagcag aagctgaacc ttatgagcaa
     1381 tatgatacat ttagttatga cgaggatgat gaatttgaaa caataacaga tgaaaagaca
     1441 gatgagtttt tagaaagaga agccgaaaaa actgaagata aaggccccaa aaaacctcag
     1501 attaaaaagt taaaaagcgg caaaaagcct aatttattta acaaaaaaga ggacatatta
     1561 aggggcataa taatgaagga agtattagat caaccccgtt acaagaaaaa gaagaataaa
     1621 taaccgaaga aatgtaatat attaaaacta ataagcccca atatggggct tttcttctgc
     1681 atatagcctt taatatttat ttatttaatt ttatattttt cattaatgta ataattaaat
     1741 ctaattgatg agatagtgtt gatataatat tttaattatg ttatattatt tacaaatatg
     1801 actaaaaaac aggaggggct atatggagtt atcaaaaaga caaaaagaaa taattgaaat
     1861 agtaaaaaat cagggaccga tttcttccaa gaaaattgcg gatcaattag gtttatccag
     1921 agctactatc cgctctgatt tatcaatttt atctatgctt aagattttag atgctaaacc
     1981 aaatgtaggt tactatttac tgcaagaaga ttaccaatta agagaaatag aaagacttta
     2041 taaaagaaat gttaaagaaa taatgtctga ccctattcat gtaaaagaaa aaacaagtat
     2101 ttatgatacg atagttaaga tgtttctaga agatgttggc tccatagttg ttgtaaatga
     2161 agaagaaaaa ttgagtggag ttatctcccg aaaagactta ctcaaaatga gtattgggca
     2221 aagtaattta aaaaacactc cggtcagttt agctatgaca aggcggccga agattatcac
     2281 agtatctcct gaaaccactt ttatagaagc cgcccgcaag ataaggtacc ataagattga
     2341 ttttctaccc gtaattactg agaatgatga ggttattgga cggataagta aaaccagcat
     2401 aattaaagcc tttgttgatt atgctgagta aagatgtaag tataaaataa tttcccatct
     2461 tttatattaa tcagttatat tcaaaagata taatcataag caaaatcaac tgtgattatt
     2521 atgtttaact tgtgtttttt tagatagagt acatattaat tgttgatatt aatataatat
     2581 attattaaaa ataaagaatt atatagctga ttattgataa aaaattaata gataattaaa
     2641 tgccagtata tagccttcat tttagctata gactggcttt ttatgtttgt aaaacttgta
     2701 agattactat aatgtgttat aatattctta gtatgttttt ctaataagaa agtataacca
     2761 aataatggag gaatatttta tttcctcttg aataaaataa aagtaggtta aaaattataa
     2821 attgaggagg gatccggtgg taccggttga agaacagata gatataaatg attatcaagt
     2881 ttcaagaaat gtttttggca ataaggacag taacattaaa attatagaag acaagttaaa
     2941 tgtaaagatt gttgcaagag gtagtaatgt gaaaatagaa ggtaaaaaag atgatgttga
     3001 tttgaccaag cgagtaattg aagaaatgat aaaacttgct aaaacaggta agaatattga
     3061 taaaagacag gtgaagtatg ccgttgatct tctaagtaaa aataggcata tggatctaag
     3121 tgatttatat gatgaggttt tacaggttaa ctttaaagga agagaaatta aagttaagac
     3181 attaggacaa aagtattatg tagaagcaat caaaagttca gatatagtct tttcaatagg
     3241 tccggccgga acaggtaaaa cttatcttgc tatggtgatg gctgttaaaa gcttattaaa
     3301 taataatgct aatcggatag tattaacacg ccctgcaatt gaagcgggcg aacagctggg
     3361 atttttaccg ggagatttac aggataaagt tgatccttat ttaaggccgt tatatgatgc
     3421 gttatatgat attctgggac cagagaaagt tagtgaatac ttagaaaagg acataataga
     3481 ggtagctcct ttggcatata tgagaggtag aacattagac gattcatttg tgattttaga
     3541 tgaagctcaa aataccagtc cagaacagat gaagatgttc ttaacaagga taggttttaa
     3601 ttctcatgca gttataacag gtgatataac ccaaatagac ttacccagaa gctcgaattc
     3661 tggccttatt caggttagaa aaatattaaa agatattaaa ggtattgagt ttgtttattt
     3721 aacaaaccgg gacgtagtaa gacatgaatt ggtaaaagat atcatcaaag catacgaaag
     3781 gtatgaaagt gggaattaac tatgcaattt tttgaacgaa tcaaaaatcg gttcgataaa
     3841 tggaccgatt ttgatataaa actaaatgat atctataaaa gaagaataag tttagtcatc
     3901 ttctttatag tgattgcctt aattttgacc tttaactttt ttccaaatca aattgatctg
     3961 gaagtaggtc aggtggcaaa aagtgatatc gtggcaccaa ggactgtaac atttatcgac
     4021 caggagaaaa ccgaacagtt aagagaactg gctgttgaat ctgcttctaa ggtttatgag
     4081 gaagataagt cagttaacag gagaatttta aatgaaatag ataatctctt taatactgtg
     4141 gttaatacta aaacagaaat gagcagtgat agtaccggcc agataaataa tcaattttta
     4201 acagaggtaa agaataataa tgatctaaac attaataata acgaccttaa tttactactt
     4261 aaaacaaata tagataaatt aaacagtctc cgcagttcag ccaatgatat catgcaggaa
     4321 gtattacagg aaaggatatt tcctgaaaat ataatagaag tgagaaataa aataaatcaa
     4381 cttgctttag agaccaactt tgaaaaagaa tacaggttgg ttttagtgaa tatactaaca
     4441 aaccatgttg agcccaatat gatattagat ctagatgcaa ctgagcagag acagcaggaa
     4501 gccttaagac aagtagaacc ggtaaaaagg accgttctaa aaggagagaa tatcattcgg
     4561 aaaggtgata ttgtctcaca agatgatata caggtattag aggccttagg actcaggaaa
     4621 ccgagtataa attatgttaa cttattggga gttttaatta ccttaagtgt attagtcttt
     4681 ttattgggat attatataaa acattatgag aaagaagtat gggaaaatat caataaatta
     4741 attttgattg agactttaat aatacttgtc attttactgg caaagattat tagtattttt
     4801 caaccgacct acatggttta tctcgtacca gctgcaattg cttctatttt aataactgtt
     4861 ttaatcaata cagaaattgc gctagtagtt acagtcttta ttagtttttt aactgcatta
     4921 gtatttagta atctatatat tgcagccgca gccgcctttg tgggtggttt agtcggtata
     4981 attagtgtta gtaatgtaga tcagagaagt gatcttattc gagccggatt caatgtcagt
     5041 gtttccttgg ttgttttcat cgcaggttta actatgatgg atttaactaa taactggttc
     5101 gaattattat ggtcagcctc gatgggtatc ttaaatggta tttttgtggc tattctggcc
     5161 aacggcttat taccttatct ggaaaatatc tttgatttaa cttcttcggt aaagttactg
     5221 gagttatcaa atccaagtca tcccttatta aagagactgc ttgttgaggc acccgggacc
     5281 tatcaccaca gtataatcgt aggtaatttg gctgaaaatg cagccgataa gattggtgcc
     5341 gattcactct tgacaagagt gggagcatat tttcatgata taggtaaatt aaaaagaccc
     5401 tatttctttt cagacaatca gtttggggga gaaaatccgc acagcaagac ctctcccaat
     5461 ttaagtgcat tgataattaa gtcccatgta aaggatggtt tagaacttgc agataagtat
     5521 aatctaccta aggctgttaa agacatcatc caacagcacc aaggtacagg cctaatatca
     5581 tttttctata aacaggcgat agaaaaaagt aagcacggta atattaatga gagcgatttt
     5641 agatatgatg gtcctaaacc tcagtctaaa gaagcggctc tcataatgtt agcagatact
     5701 gttgaggcgg ctgtaaggtc caagaacttt aataaaaata accataacag gattgaactg
     5761 tttgttaaag aattaataag agagaagcta aatgatggtc agttagatga atcctctctt
     5821 acattacatg agttagatga aattgcagaa agctttgttc aaatcctgac tggtatttat
     5881 cataaacgta ttgaatatcc tgataattta ataaaagaaa ttaaaaagga tgataaaaat
     5941 gataaaaatg gaaatcaatg atctgcagga taaagtagag attgaagaag atctctatga
     6001 agacttcaaa aagattatta caaaggctac agagatggaa ggatatgatg gtggagagat
     6061 aagtattgct tttgttaata atgaaaagat aaaggaatta aacaataaat tcagaaatat
     6121 aaatgaagta accgatgttt tatcatttcc catggatgaa gagatattag gagacatcat
     6181 aatttcagcg gagagggctc taagtcagtc caaggattat ggtcattcct ttaaaagaga
     6241 aatttgttat ttggtgacac atggtatcct acatctatta ggttatgatc ataaaactga
     6301 tggagaaaaa gaggaaatgc gtaaaaaaga agaaagaata ttaaaagagt tagaaattaa
     6361 aagatagtta aaatttgata aaaaagatgt atttggctat tgaactggag ggcattaaag
     6421 aatgtatatt aatttgattg gctttgtttt tttacttatc ctttctggat tcttttcagg
     6481 ttcagagaca gcttttatgt ctgccaacag ggtaagaatt agagaactag ccaatgaagg
     6541 tgacaaacaa gctggaagag ttgacaacct tttagaagat caaacaaaat tattaacaac
     6601 aatcttaata ggaaataact tagttaatat cgcagcttct gcaatagcta cttctattgc
     6661 cataaatatt tttggtagta aaggtgtagg tattgcaacc ggttttgtta ctttagtcat
     6721 attagttttt ggtgagatca cacccaaatc tttaggtaat aacagtccaa ttgcatatgc
     6781 taaaaaagca tcgataatat tatattggtt agaaaaaatt ctatatccgg ttatcttatt
     6841 tttcaccatc ttgattaaaa agatgattgg tgaaaacaag atgatctctt ctgcttttat
     6901 tagcgaagaa gaagtaagga gattcgttaa tgtaagtgaa gaagaagggg ctataaaaga
     6961 aagcgaaaaa gaaatgattc aaagtgtctt tgaattcgat gatattgtgg taaaagaaat
     7021 tatggtcccc cgcatagata tggtatgtat cagcaaagat gcaagcatca atgagcttgt
     7081 tgatttagca gtagaaaaag gccactcccg tattcctgtt tatgaggaaa gtatagatga
     7141 tatcattggt ctaatttatg ttaaggactt attacagtta ctacagaaag gtaaagaaaa
     7201 tctaacttta gaagatttaa ttaaaccaat ctactttata ccggaaagta aacagatcaa
     7261 taagttatta aaggaaatgc agaatagaag agagcatatg gcagttatat tggatgagta
     7321 tggaggtact tccggcttaa taaccattga agacttatta gaagagatcg ttggtgatat
     7381 tcaggatgag tttgatttag aagataaaca gattaatttg atcaataata aagagatcct
     7441 ggtagatgca agagtagatt tagatgattt aaatgagata ttaccaaccc ctttacttga
     7501 tgaagaaagt tatgagacaa taagtggttt tgtcttacat aaattaggat atttacccga
     7561 agagaaagag actatagaat tagaaggtgt gaccatcgaa gttgaaaaaa tagatgaaca
     7621 ccggattcaa aaacttcgta tatatactgt agagccgata aagaaaatat aaattagatt
     7681 agaaactgta tattctgtat tgagaattga tgttaaagat aaattaagaa ctgtaggggt
     7741 gtaaagatga aaaaaggctt attatatgga ctagtaatat tattagtaat tgtggcagta
     7801 tctgctgtct tattaagggg aaggttagat gaagaaacac ctgagatgac tgagcagcag
     7861 gtgacagaag aagcaccctc tgaggaagaa agcagtcaaa ctgatcaaga tagcgaagaa
     7921 caagaagaac aagaagtaga gaaagaaacc gaagatgaaa aagaagagga gtccgaagaa
     7981 gagacagagg acgatcaaac tgacgaagaa caagatgccc aagaagaaga tgtgatctta
     8041 tctaagttta atcacttacc aatcgaagag aaagttctgc atcgggctgt catggttagt
     8101 gtagaaaaca ccccgaaagc cagaccacaa tctggtcttt gtcaagcacc aatcgtttat
     8161 gaatttttag tagaaggcgg aattacaaga tttttggctt tattttggtc cgatattccg
     8221 ggcaaaattg gtcctattag aagtgttaga ccttatatga tagagattgc tcaggaatat
     8281 gatgctttgc ttttacatgc aggggccagt ccagatggat ttgagatgct tgataaaata
     8341 gactatacaa atttagatca aatatacaat ggtaagtact actggaggag ccaacagaga
     8401 cccactcccc ataatttata tacaggtgaa tataggataa ataattattt agaagatatg
     8461 ttagggattg aatatgaaaa ccgctttaac tttgaagaaa taatgattat ctccaatgcg
     8521 aaagcaggag cggaagagat cagtattgac tattggggta attatgaagt cttatatgaa
     8581 tataaccgag aaaacaatta ctataaaaga tttttaaatt caaaagataa cccccattta
     8641 tgtggtgatg aagaacaaat aacagttaaa aatattattg tacagtttgt tcaaacggaa
     8701 gttaaagatg atgtagggag attaagtatg aagttggaag gtgaaaataa ggcattatac
     8761 tttagaaacg gagttgttag tgaaggtcac tggcaaaaaa atagttcagg attaacaaaa
     8821 ttttatgatg aaaacgatca attaatcact gtaaatcctg gtaatacatg gatccagatt
     8881 gtgccgaata atactaatat aacatataag gaaagtgatt aattatgata gataaaaaga
     8941 ctaaaaataa attattaaaa gctgcaaaag aggtaaaaga aaacgcatat gtaccttatt
     9001 ctaatttccc cttggcagca gctttattaa caaaggaagg caagatattt accggtgtaa
     9061 atattgaaaa tgctgcttat ggagtaacta tgtgtgctga aagaacagct atctttaaag
     9121 cggtttcaga aggttatacc gaatttgaag cactactttt attatcaaat accgaaaaac
     9181 ctatcacacc ctgtggttct tgccgacagg taataagtga atttagcaat gatattgata
     9241 taataatgac tacttatgat aatggtgaat taattaagaa aaataaagaa cttttaccgg
     9301 gttcatttaa taaggatgat atgaaataat gagttttaaa tcaggatttg taacgattat
     9361 tggaagacct aatgtaggta aatctacctt agtaaattat ttaattggtg aaaaaatttc
     9421 cataacctca ccccgacccc agaccacgcg taatagtatt aaagcgatat atacagaaga
     9481 tgcgggccag ataatcttcg ttgacacccc gggtatttac aagactaaaa atgacctgga
     9541 taaatatatg gtcaacgaag cttataagag cttagaagga atagatgtaa taatatttat
     9601 gctggatgcc acagagaaat tccaaaaagg ggatcaaatt atctataatc agattaaaaa
     9661 ttcttctaat ccctttttaa tagttttgaa taagatagac aaggtaaaaa agaaagaatt
     9721 acccaatatt cttaaagatt ataataaaag aattgaagaa aacataatac caatatcctc
     9781 taaaacagga agaaatgtca ataatctaat aaatgagata tttgaacttt taccggccgg
     9841 tccccaatac tatcccgatg atatgataac tgaccaaatt gagcagttta tagttgcaga
     9901 aatgattaga gagaaaacat tttacctact aagagaagaa gttcccttcg gtattgccgt
     9961 ggttattgaa gaaatgaaag aacgaaatga tgaaacgatg tatataagag ctaatatata
    10021 tgtagagaaa aaatcacata aaggtataat tatcggaaaa aacggcaaaa tgctcaaaaa
    10081 aataggcacg agagcccgca aagatgtaga aaaattatta cagcaaaaag tctatcttga
    10141 cctttgggtt aaaatcgaga aggactggag ggatgatgaa aacttattaa gaagaatggg
    10201 ttataagggg tgattgaatg gtagaaaaat catatagaga ctttattcca g
//
LOCUS       DATGCJ010000136        13342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_240268, whole genome shotgun sequence.
ACCESSION   DATGCJ010000136 DATGCJ010000000
VERSION     DATGCJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240268"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="dxr"
                     /locus_tag="VKN64_06890"
     CDS             <1..978
                     /gene="dxr"
                     /locus_tag="VKN64_06890"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HMA60002.1"
                     /translation="ESSAKEVKLNLRDYNIKVLEGQKNINEIVQLQETDLVINALVGA
                     AGLIPTLNTLKQGTKLGLANKESLVIGGHLVKKQLEKINKDNMLLPIDSEHNAIFRLL
                     EEHSKKELKNIILTASGGPFVNKSKKEMNKVTVKEALDHPNWDMGAKITIDSATMMNK
                     GLEVIEAHWLFGVGYDKIKVVIHPQSIIHSMIELIDNSIYAEMSVADMRMPIQHVMEY
                     PDISSGIGEPLNLVEQGKLEFLKPDYDKFECLDLAFKAGKKGGSYPVVLNAANEIAVN
                     KFLNKKIKFVQIPKIIRETIEAHNKVDNPNIDEIIEIDKWARQFVKEVV"
     gene            980..2053
                     /gene="rseP"
                     /locus_tag="VKN64_06895"
     CDS             980..2053
                     /gene="rseP"
                     /locus_tag="VKN64_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMA60003.1"
                     /translation="MFITILSFIIVLGILIFIHEFGHYITAKLSDIRVEEFALGFGPK
                     LIYRQWGETVYSIRAIPLGGFCNMTGEFVPDEDDDEEDIEIYNEAKANGRCFHQKPMW
                     KRFAVIFMGPFMNFMLALFIFFLIFSLAGVPIDTSNQPIIGELDPSRPASEAGLLPGD
                     RIVEIEGQRVQSWEDMTGIIHNSKTEPLEIVIERDGATRTFNITPVRNEEMGVNIIGI
                     YPQVVRENIGFFGSLQRAVVQGWRVFYLTINGFIQMISNRSMEGIGGPVMIASIIGQA
                     ARNGIINVMNWTAIISINLGIINLLPLPALDGGRILFIVIELVRGKPVDPKKEGFVHL
                     IGFVLLMILMLFIIYNDIARNFF"
     gene            2068..3141
                     /gene="ispG"
                     /locus_tag="VKN64_06900"
                     /gene_synonym="gcpE"
     CDS             2068..3141
                     /gene="ispG"
                     /locus_tag="VKN64_06900"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HMA60004.1"
                     /translation="MNERRKTRNIFYKNTGVGSDFPITVQSMTNTKTDNVRETVSQIH
                     RLEKAGCEIIRVAVPDKKAALSISDIKKEISIPLIADIHFNHELALMSIEQGVDGLRI
                     NPGNIGDKKKISAVVKKAKKQDIPIRIGVNSGSIEKDLLKKYVKPTAEGMVESALKHV
                     KILEELDFKDIIISMKSTDVLMTLKAHQLLNQEVDYPFHIGITEAGSGKEGIIKSSVG
                     LALLLNNGFGDTLRVSLTGDPVEEVYTAFEILRNLKLRNRGINIISCPTCGRTEVDLA
                     RITNEIKKRIKDYHKYFEDGLTVAVMGCEVNGPGEAREADLGLACGKKSGLMFKKGEV
                     IKKVKEEDMIETLLEEIKKMRSE"
     gene            3145..4854
                     /locus_tag="VKN64_06905"
     CDS             3145..4854
                     /locus_tag="VKN64_06905"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMA60005.1"
                     /translation="MRMSKLYLPTLKETPAEAEIKSHQLMLRAGMMRKLSPGIFSYLP
                     LGYNVIKKIENIVREEMDKTSSQEVLLPVMQTAEIWKESNRWQKFGPLMIKFTDRKDR
                     EYCLGPTHEEVITDLIKDEVRSYKDLPLNLYQIQTKVRDEIRPRFGVMRGREFTMKDA
                     YSMDTDYEGLDESYQEMYDAYVKIFERLGLKTRVVKADTGAMGGKDSHEFMVLAESGE
                     DDIAICDNCHYAANTELASAYDQEKIVDEDQKERKIIDTPDIKTIEGLENFLNIDGSE
                     MVKTMLMKADDQLVVVLVRGDDQLNEVKLSNYLQATTLEAANEEEIIEVFGVKPGFIG
                     PVDIEGYQIIADHRIKGMRNAVCGANIEDKHFGNVNPGRDFEVDEYLELRTVKGGDTC
                     PECQTGSLAIQPGIEVGHIFKLGTKYSESMGATYLDENGKAQPVVMGSYGIGITRIVA
                     AAIEQSNDENGIIWPKAIAPYQIIILPLGNSEEVEETAEEIYEKFTEMGIEVLLDDRD
                     ERAGVKFNDADLIGIPLRITVGSRSLNNNVLEVRNRQTGEDFEVSLDNYEEEIKDFLS
                     KLK"
     gene            4870..5505
                     /locus_tag="VKN64_06910"
     CDS             4870..5505
                     /locus_tag="VKN64_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA60006.1"
                     /translation="MKIKTLIPAFNEEKTIGDIIDTLNQVSLINEVIVVDDGSEDNTY
                     LVAKNRGARVIKMDQNRGKGAALQEGIAKIDTDIILLLDGDLIGLRQEHVYKLLNPVI
                     SDEADMTIGVFKKGRGLTDLAQVVSPGLSGQRAIKLSELKKVKNLDKEGFGVEVALNK
                     HFKEKGIVKKVELKDLTHVMKEEKMGLVKGVKARTKMYIDILRSLFTGIID"
     gene            5529..9701
                     /locus_tag="VKN64_06915"
     CDS             5529..9701
                     /locus_tag="VKN64_06915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HMA60007.1"
                     /translation="MLKVIQPGEDYKDKLKYIIVDSEKKICKVVLNKNGDNEQIEDKV
                     GSLLPNFSIDCEVALSKEEEIEMIWPQLTKEFNEKFSYSKTWFERAKLFFENDRLIIS
                     LETKLAAKKMSKARVINFIKNRIYYYLDQELDIRIENGDFFKNDKSLKKDLEKEQAAK
                     SKKRQKKRKSKNKIIYGGKINNGKNKVDLDQIKSEQRNITTSGFVFDQEVKQIRKNLT
                     LHTFHITNFKDSLTCKAFLNNNGQKLNIKEDMEVLVTGNIQYDKYSKDLILMADRINK
                     IGEKETREDKSDQKRIELHLHTKMSAMDSTVDVENAVKRASKWDHAALAITDHGIVQS
                     YPDAYQAGQKYGVDIIFGLEAYLIDNGKPIVVNPSDQTINETEFVVFDLETTGLNPVN
                     NEIIEIGAVKLKDGKVIDEFSSMVKPERNIPAKITEITGIDDSMVKDAEPFEKVFRKF
                     IDFIGGAPLVAHNMDFDYSFIREPLNKFMRSDEVTLIDTLTLARAVKPKLKNHKLATL
                     VDHYNIELDNHHRALDDAKATGYLLLELLSEEESEELDNINELNELTKNIDWKKQRPY
                     HALVLAKNQAGLKDIYKLVSKAHLDNFYRKPRILKSELLAKRENLLIGSACESGQLFN
                     YILNNRSSKEIKEVVKFYDFLEIQPLGNNEFLISNQLEDKNELKKINRRIYELGKKFK
                     KPVVATGDVHFLDPKDEIYRKILQAGQGFDDYDNQAPLYYRTTDEMLEEFDYFSEEEA
                     HEIVVENPNKIYEKIEEIKPIPDGLYTPDIEGADEKIKNMSYNKAKKLYGEDLPEYIE
                     DRLEKELNSIIDNGYSVIYLIAHKLVKRSLEDGYLVGSRGSVGSSFVATMTDITEVNP
                     LPAHYRCENCQNVEIKQEEKYSTGIDLPDKKCPECGEQYTKDGFDIPFEVFMGFKGDK
                     VPDIDLNFSGEYQNTIHEATEEFFGRDYVFRAGTISTIADRTAFGFVKNYLDDNDIDV
                     KKAEINRLVDGCAGVKRTTGQHPGGLMVVPKNMDIYDFTPIQHPANDQETRVRTTHFD
                     YHSISGRILKLDLLGHDDPTSLRMLQDLTDVDPETIPLDDPETMKIFSSTESLGVSKE
                     KINSEVGTYGIPEFGTSFVRQMLIDTRPSTFAELVRISGLSHGTDVWLNNAQELIKND
                     KATLSEVISVRDDIMNYLLQKGLEPIDAFWIMEHVRKGKGLTEKEEQKMIDKGVPDWY
                     IDSCKKIKYMFPKAHAAAYVMMAYRIAYFKVHYPKAFYLTYFSTKASAFDAQIIDKGL
                     EFVKDYLDKLNEQDKLTAKDKETITILEVVIEAMHRGVEFGKVDLYNSKKNKFILRDE
                     KLVPPLICLEGLGSSAAESIIEARAQGKFISIEDLTNRTSLSKTVIEKMKDHGTLKGL
                     PEKNQLSLFASS"
     gene            9805..10275
                     /gene="rimP"
                     /locus_tag="VKN64_06920"
     CDS             9805..10275
                     /gene="rimP"
                     /locus_tag="VKN64_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HMA60008.1"
                     /translation="MSVVDQLRELIEPIVKVNDLDFVDIEYIKEGENWILRVFVENEE
                     GELTIDQLSNLSRLISQKLDEEDPIDKRYFLEVSSPGVERPLRKVKDYERFAGEDIKI
                     STYRKINNSKEFIGKLIGIDENKVITIKLKDTDEEMEIDYSDVSKANLHEEFNF"
     gene            10298..11467
                     /gene="nusA"
                     /locus_tag="VKN64_06925"
     CDS             10298..11467
                     /gene="nusA"
                     /locus_tag="VKN64_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMA60009.1"
                     /translation="MNLDLLNALDDIEKDKGISKEVLLEAIEVALLSAYKKDFGSKEN
                     VSVEIDEESGEVMVFSRKKVVEEVKNDRYEIPLDEAERIDSSIEVGDYLDVEVTPENF
                     GRIAAQTAKQVVMQRIREAERDVLYEEYKRKEGEIITGTIQRFHKDNVLIDMGKTEAL
                     LPVSEQMPSEEYRVGSRLKLYVVEVSSTTKGPRILVSRTHPALLKRLFEVEVPEIFDG
                     IVEIRSVAREAGFRSKIAVSSYDDKVDPVGACVGPQGMRVQSVVEQLNGEKIDIVQYS
                     EDPKEYVSNALNPAEVIDVVTDEDQQSAKVIVPDFQLSLAIGKEGQNARLAAKLTGWK
                     IDIKKESEFTEDETNEEEEETEQESKEETESLDSAEPQAEVEQQPEDSQEDETDK"
     gene            11496..>13342
                     /gene="infB"
                     /locus_tag="VKN64_06930"
     CDS             11496..>13342
                     /gene="infB"
                     /locus_tag="VKN64_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMA60010.1"
                     /translation="MSKIRVYKLADKLNMESKELIDILNNLGIEVSSHMSTIEDETAD
                     LVKEVVTEESKSEEEIEEELEDELPEESAEEQETEPEIEEEEIEEEDESDYIPVTEGI
                     TIRDLGEKLDISANNIIKEMMQMGIMTNVNYSLDEDELEILKEELGLEIKLTDEIEEE
                     ESSELKLEEIVDRPEDLKPRPPIVTVMGHVDHGKTTLLDVIRKTRVAEGEAGGITQHI
                     GAYQASYDEKKITFIDTPGHEAFTAMRARGAQLTDIVILVVAADDGVMPQTVEAINHA
                     QAAGIPIIVAVNKIDKANAQPDRVKQELTEHSLVPEEWGGNTICVNVSALKGENIDEL
                     LEMVNLVAEVEELKANPNRIADGVIIESELDRGRGPIATVLVKNGTLKIGDYIIAGSI
                     TGRVKALLDEHGERIDEAGPATPVIVLGFDDVPEAGDYVRVMEDEKRARDLAERRQEK
                     EKEERLKKDSKVSLEDLYRNIQEGEIKELNVIIKADVQGSIAALESSLMNIESDEVSV
                     NIIHTGVGAINETDVNLASASQAIIIGFNVRPGTNARKAAEKEQVEIKTYRVIYKVID
                     DIKNAMSGLLEPEYKEIVVGRVEVRDTFKVPDVGIIAGAYVKEGEVNRN"
BASE COUNT         4815 a         1992 c         2931 g         3604 t
ORIGIN      
        1 gaaagtagtg ctaaagaagt aaaattgaat ttaagagatt ataatattaa agtattagaa
       61 ggacaaaaaa atattaatga aatcgttcaa cttcaagaaa cggatctggt tattaatgct
      121 ttagtaggcg cggctggtct aattcctact ttaaatacac ttaagcaggg tacaaaactg
      181 gggttggcca ataaagagag tttagttatc ggtggacacc ttgtaaaaaa acagttggaa
      241 aagattaata aagataacat gttattacct attgatagtg aacacaacgc gatctttaga
      301 ttattagaag aacattctaa aaaagaatta aaaaatataa ttttaaccgc ttccggcggt
      361 ccttttgtta ataaaagtaa aaaagaaatg aataaggtta cagttaaaga agccttggat
      421 catcccaact gggatatggg tgccaagatt actatagatt ctgctaccat gatgaataaa
      481 ggcttagaag taatagaagc acattggtta ttcggggttg gatatgataa aataaaagta
      541 gtaattcatc ctcaaagtat aatccactcc atgatagagt taattgataa ttctatatat
      601 gctgagatga gtgttgccga tatgagaatg cccatccaac atgtaatgga atatccggat
      661 ataagctcag ggataggtga acctttaaat ctagtagagc agggtaaatt agaatttcta
      721 aaacccgatt atgataaatt cgaatgttta gatttagcat tcaaagctgg taaaaaaggt
      781 ggtagttatc cagttgtttt aaatgctgcc aatgagatag ctgtaaataa atttttaaat
      841 aaaaaaatta agtttgtaca aataccaaaa ataataagag aaactataga ggctcataat
      901 aaagtagata atcctaatat cgatgagatt attgagatag acaaatgggc cagacagttt
      961 gtaaaggagg tagtttaata tgtttattac tattctcagt tttataatag tactgggaat
     1021 attaattttt atacatgaat tcggccatta tataactgct aagttaagtg atatcagggt
     1081 agaagagttt gctttaggtt ttggccctaa acttatctac aggcagtggg gggaaacagt
     1141 ttattcgatt agagcgatac ccctgggcgg attctgtaat atgaccggtg aatttgttcc
     1201 cgatgaagac gatgatgagg aagatattga aatatataat gaagccaaag ctaatgggag
     1261 atgtttccat caaaaaccta tgtggaagag gtttgctgtt atcttcatgg gaccattcat
     1321 gaacttcatg ttagctttgt ttatcttttt tctaattttt agcctggccg gagtcccgat
     1381 cgatacttcc aatcaaccca ttattgggga attagatccc agtagacccg cttctgaggc
     1441 cggtctttta ccgggagata gaatagtaga gatcgaagga caaagggttc aaagctggga
     1501 agatatgaca ggcattattc ataattcaaa aacagaacca ctagaaattg tgatagaaag
     1561 agacggagcg acgaggacct ttaatataac acccgtccgt aatgaagaaa tgggtgtgaa
     1621 tattattggt atctatcctc aggttgtgcg agaaaacatc ggcttttttg gttcattaca
     1681 acgcgctgta gttcaaggct ggcgggtctt ttatctaacg attaacggct ttatccagat
     1741 gatctctaat aggtcgatgg agggtatagg tggaccggtt atgatcgcct caataattgg
     1801 acaggctgcc cgtaatggaa ttattaatgt gatgaactgg acagccatta tctccattaa
     1861 cttaggtatt attaacctgt taccattacc ggcactggat gggggtagaa tcctctttat
     1921 agttatcgaa ttggttagag gtaaacccgt agatccgaaa aaagaaggct ttgtccattt
     1981 aatcggtttt gtccttttaa tgatcttgat gttattcatt atttataatg atattgcccg
     2041 taacttcttt taaagtggtg tatttatatg aatgaaagaa gaaaaacaag aaatatattt
     2101 tataaaaata ctggcgtggg cagtgatttt cccatcactg tccaatccat gacaaatact
     2161 aaaactgata atgtaaggga aactgtaagt cagattcaca gattagaaaa agccggctgt
     2221 gaaattatca gagttgctgt tcctgataaa aaagcagcct tatctatatc tgatattaaa
     2281 aaagagataa gtatccccct tatcgccgat attcacttta atcatgaatt ggctttaatg
     2341 tctattgaac agggtgtaga tgggttacgt attaaccccg gtaatattgg ggataaaaag
     2401 aaaatatcag ctgtcgttaa aaaggcaaaa aagcaagata tcccaattag aatcggtgtg
     2461 aattccggtt ctattgaaaa agatctatta aaaaaatatg ttaagccaac ggccgaagga
     2521 atggtggaaa gcgccctaaa acatgtaaag atattagagg aactggattt taaagatatt
     2581 atcatttcaa tgaaatcgac tgatgtttta atgaccttaa aagcacatca acttttaaat
     2641 caagaggtcg actacccctt ccatattggt attactgaag caggcagcgg caaagaaggt
     2701 ataataaaat cttctgtggg tttagccctg cttttaaata atggatttgg tgatacactg
     2761 cgggtctcac ttaccgggga tcccgtagaa gaagtatata ctgcttttga gatattaaga
     2821 aacttaaaat taagaaatag ggggattaat attatctcct gtccaacctg tggtcggacc
     2881 gaggtagatc tagccaggat cactaatgag attaaaaaaa gaattaaaga ttaccataaa
     2941 tattttgaag acggacttac agttgctgta atgggctgtg aagtaaatgg acctggggaa
     3001 gcaagagaag cggacctggg cttggcctgt ggtaaaaaat caggcctcat gttcaaaaag
     3061 ggagaagtaa tcaaaaaggt caaagaagaa gatatgattg aaacattact agaagaaata
     3121 aaaaagatga ggagtgagta agaaatgcga atgtcaaaac tatatctacc aactttaaag
     3181 gaaacacctg ctgaagctga aataaaaagt catcagctga tgttaagagc aggtatgatg
     3241 agaaagttaa gtccaggtat tttttcttat ttacccctgg gttataatgt aatcaaaaag
     3301 atagagaaca tagtaagaga agagatggat aaaacttctt cccaggaagt actattacct
     3361 gtcatgcaga ccgctgagat ctggaaagag tcaaatcgct ggcagaagtt cggtcccttg
     3421 atgatcaaat ttacagatcg aaaagaccgc gaatactgtc tgggtccaac ccatgaagaa
     3481 gtgataaccg atttaattaa ggatgaagtt agatcttata aagatcttcc cttaaatctt
     3541 tatcaaatcc agacaaaagt ccgtgatgaa atcagaccgc gttttggagt aatgcgcggc
     3601 cgtgagttta caatgaaaga tgcctatagt atggatacgg actatgaagg gctggatgaa
     3661 agttatcagg aaatgtatga tgcctatgtt aaaatatttg aaagacttgg tttaaaaact
     3721 agagttgtaa aggcagatac cggagcaatg ggtggtaaag actcacatga attcatggta
     3781 ttagcagaat ccggtgaaga tgatattgcg atctgtgaca actgccatta tgctgccaat
     3841 acggaattag catctgccta tgatcaagaa aaaatagttg atgaagatca aaaggaacgt
     3901 aagattattg atacccctga tattaaaaca attgaaggtt tagagaattt ccttaacata
     3961 gatggttccg aaatggttaa aacaatgttg atgaaggctg atgatcagtt agtagttgta
     4021 ttagtacgtg gtgatgatca gttaaatgaa gtaaaattat caaactatct acaggcaaca
     4081 actttagaag ctgccaatga agaagaaatt attgaagttt ttggagttaa acccggcttt
     4141 atcggtccgg ttgatataga aggttatcaa ataattgctg accatagaat caaagggatg
     4201 agaaacgcag tctgtggggc aaatatagag gacaagcact tcggtaatgt aaaccccggc
     4261 cgtgactttg aagtagatga atacttggaa ttaagaactg tcaagggggg tgacacctgt
     4321 cctgagtgcc agacaggttc cttagccatt caacccggta ttgaagtggg gcatatcttt
     4381 aaactgggaa ctaaatacag tgaaagtatg ggagctacct acttagatga aaacggtaaa
     4441 gcccaaccgg tcgtaatggg tagttatgga ataggtatta ccagaattgt agcagccgca
     4501 attgaacaga gcaatgatga gaacggtatc atttggccga aggcaatagc tccttatcaa
     4561 ataataatat tacctcttgg taatagtgaa gaagtggaag aaacggctga agaaatctat
     4621 gagaagttta ccgaaatggg tatagaagta ttactcgatg accgtgatga aagggccggt
     4681 gttaaattta atgatgctga tctaattggt attccgctca gaataaccgt gggcagccgt
     4741 tccttaaata ataatgtatt ggaggtcaga aaccgccaaa ctggtgagga cttcgaagtc
     4801 tctttagata attacgaaga ggagataaaa gactttcttt ctaaattaaa ataagagagg
     4861 ggcagtccaa tgaagatcaa gactttaatt ccggccttca atgaagagaa aaccatcggt
     4921 gatattatcg ataccttaaa tcaagtatca ttgataaatg aggtaattgt ggttgatgat
     4981 ggttctgagg ataacaccta tctggtagca aaaaataggg gcgcccgggt aataaagatg
     5041 gaccagaacc gcggaaaagg tgccgcttta caggaaggta ttgctaaaat tgatacagat
     5101 attatcttac ttttagatgg ggatttaatt ggattaagac aggaacatgt ctataaactg
     5161 cttaatcccg ttatttcgga tgaggcagat atgacaatag gggtatttaa aaaagggagg
     5221 ggcttaactg atttagctca ggtggtttct cccggtctct ccggtcaaag ggctattaaa
     5281 ctatctgaat taaaaaaagt aaagaattta gataaagaag gttttggagt tgaggtcgcg
     5341 ttaaataaac acttcaagga aaaaggtata gtaaaaaagg tagaacttaa agatttaacc
     5401 catgtaatga aggaagaaaa gatgggctta gtaaaagggg taaaagcccg cacaaagatg
     5461 tatattgata tactgagaag tttatttacc ggtataattg attaattagt tcttttagga
     5521 gggattttgt gcttaaagtc atccaaccag gggaagatta taaagataaa ttaaaatata
     5581 ttatagttga ttccgagaaa aaaatctgta aggttgtatt aaataaaaat ggtgataatg
     5641 aacagattga agataaagta ggcagtctat tacctaattt ttcaatagac tgtgaggttg
     5701 ctttatcaaa agaagaagaa atagaaatga tctggccgca gttaacaaaa gaattcaacg
     5761 aaaagttttc ttatagcaaa acctggtttg aaagggccaa attatttttt gaaaatgaca
     5821 gactgataat ttcactagaa acaaaactcg cagccaaaaa gatgagcaag gcccgggtta
     5881 tcaattttat aaaaaataga atatattatt acctagatca ggaattagat attagaattg
     5941 aaaatggaga tttctttaaa aatgataaat ctctaaaaaa agatttggaa aaagaacaag
     6001 cagccaaaag caaaaaacgc caaaagaagc ggaaaagtaa aaataaaata atctatggtg
     6061 ggaaaattaa taacggcaaa aacaaagtag atcttgacca gattaaatct gaacagagaa
     6121 atataacaac cagtggtttt gtatttgacc aggaagtaaa acagatcaga aaaaatctaa
     6181 cactccatac attccatatt acaaacttta aagattcact tacttgtaaa gcttttttaa
     6241 ataataatgg tcagaaatta aatatcaaag aagatatgga agttctggtt acaggaaata
     6301 ttcaatatga taaatatagt aaagatctaa tcctaatggc agatagaatc aataagattg
     6361 gagaaaaaga gacccgtgaa gataaatcag atcaaaaaag gattgaactg cacttacata
     6421 ccaagatgag tgccatggat tctactgtcg atgtagaaaa tgcagttaag agagcctcta
     6481 agtgggacca tgctgccctc gccataactg accacggcat tgtgcagtcc tatccggatg
     6541 cctatcaggc cggtcaaaaa tacggtgtgg atatcatatt tggcttagaa gcctatttaa
     6601 ttgacaacgg gaagccaatt gtagttaatc cctcagatca aactattaat gagactgagt
     6661 ttgttgtatt tgacttagaa acaaccggtt taaaccccgt caacaatgaa ataattgaaa
     6721 tcggggctgt caaactaaaa gatggaaaag tcatagatga attttcttct atggtcaaac
     6781 cggaaagaaa tataccggct aagataactg agattaccgg tattgatgac agcatggtca
     6841 aagatgctga accttttgaa aaagttttta gaaagtttat tgatttcata ggtggagccc
     6901 ctttagttgc ccataacatg gattttgact acagttttat cagagagcca ttaaataaat
     6961 tcatgagatc tgatgaggta actttaatag atactttaac cctggctagg gccgttaaac
     7021 cgaaattaaa aaaccataag ttggcaactt tggttgacca ttataatata gaattagata
     7081 accaccacag ggcccttgat gatgccaaag cgaccggata tttattgcta gaacttttaa
     7141 gtgaagagga aagtgaagaa ttagataata taaatgaatt aaatgaatta acgaaaaata
     7201 ttgattggaa aaaacagaga ccctatcatg ctttagtatt ggctaaaaat caagccggtt
     7261 taaaagatat atataaatta gtatctaaag cccatcttga taatttctat cgtaaaccga
     7321 ggatattaaa aagcgaactt ctggctaaaa gagaaaacct actgattggt tcagcctgtg
     7381 aatcgggtca gctttttaac tatattctca acaacaggag ttccaaggag ataaaagaag
     7441 tagttaaatt ttatgatttt ttagagatcc aacccctagg caataatgaa tttttaatct
     7501 caaaccagtt agaagataaa aatgaactta aaaaaataaa tagaagaatt tatgaactgg
     7561 gtaaaaagtt taaaaaaccg gtagtagcca ccggggatgt ccacttttta gatcctaaag
     7621 atgaaatata tagaaagatt ttacaggccg gacagggctt tgatgattac gataatcagg
     7681 caccgcttta ctatagaact acagatgaaa tgttagagga gtttgattac tttagtgaag
     7741 aagaagccca tgaaattgta gttgaaaacc ctaataaaat ttatgagaaa atagaagaga
     7801 tcaaacccat accggacggt ctttacacac ctgatattga aggtgcagat gaaaagataa
     7861 aaaacatgag ttataacaaa gctaaaaaac tttatggaga ggacttaccc gaatatattg
     7921 aagacagact ggaaaaggag cttaattcga tcatagataa tggctattct gttatttatc
     7981 ttatcgccca taagctggtt aagaggtctc ttgaagacgg ttatctggtt ggatcacggg
     8041 gttcagttgg ttcatctttt gtggccacta tgaccgatat aactgaggtt aatcccttac
     8101 cagcccatta tcgctgtgaa aactgccaaa atgttgaaat taaacaggag gaaaaatact
     8161 ctaccggtat agatttaccg gacaaaaaat gtcctgaatg tggtgagcag tataccaaag
     8221 atggttttga tatccccttt gaagtattta tgggatttaa gggcgacaaa gttccagata
     8281 ttgatttgaa cttctcgggt gaatatcaga atactataca cgaggcaacc gaggaatttt
     8341 tcggccgaga ttatgttttt agagcaggta caatttctac aattgctgat agaactgcct
     8401 ttggatttgt aaaaaattat ttagatgata atgatataga tgttaaaaaa gccgagatta
     8461 ataggttagt cgatggttgt gcgggcgtaa aaaggactac cggccagcat cccggtggtt
     8521 taatggtagt acctaaaaat atggatatct atgatttcac cccgatccaa cacccggcta
     8581 atgaccagga aaccagggtc aggaccaccc actttgatta ccattctata agtggtcgta
     8641 tccttaaatt agatctttta ggccatgatg atcctacttc actgagaatg ctgcaggatc
     8701 taaccgatgt agatcctgaa accattcctt tagatgatcc cgaaaccatg aagatatttt
     8761 cttctactga aagcctgggg gtcagtaaag agaagatcaa ctctgaagtg ggaacttacg
     8821 gtattccgga atttggtacc agttttgtaa ggcagatgct tatcgatacc aggccatcca
     8881 cctttgcgga actggtcaga ataagtggcc tatcgcatgg aacagatgtc tggcttaaca
     8941 atgcccagga attaataaag aatgataagg caactttatc ggaagtaatc tctgtaagag
     9001 atgatataat gaactatcta ctacaaaaag gtcttgaacc gattgatgcc ttctggatca
     9061 tggaacatgt cagaaaaggt aagggtttaa ctgaaaaaga agaacaaaag atgatagata
     9121 agggagtccc tgattggtat attgactctt gtaagaaaat aaaatacatg tttcctaaag
     9181 cccatgcggc agcctatgta atgatggctt atcgaattgc ctactttaag gtacattatc
     9241 ccaaagcctt ttatttaaca tatttttcaa ctaaagcaag tgcatttgat gctcaaatta
     9301 ttgataaagg ccttgagttt gtaaaagatt atttagataa gttaaatgaa caggataaac
     9361 taactgctaa agataaagaa acaattacca ttttagaagt ggtaattgag gccatgcata
     9421 ggggagttga atttggcaaa gtagatttat ataattcaaa aaagaataag ttcattttaa
     9481 gggacgaaaa attagtacct cccttaatct gtctggaagg tctaggttca tctgctgccg
     9541 aaagcattat tgaagctaga gcccagggta aatttatctc aatagaagat ttaactaata
     9601 gaaccagtct aagtaaaact gtgatagaaa agatgaaaga ccacggtacc ttaaaaggat
     9661 taccggaaaa gaatcaactg tcactttttg caagctcttg aaaaatagca atttttatga
     9721 tagtctgtaa tatggaatat gttgtatttt tattgaggag tgggcccgtc ccactcttta
     9781 atttatgaaa gagagggttt tactatgagc gttgttgacc aactaagaga attaatagaa
     9841 ccaatagtta aggtaaatga tcttgacttt gtggatatag aatatataaa agaaggggaa
     9901 aactggattc tcagagtatt tgtagagaat gaagaagggg aactgacaat cgaccagtta
     9961 agcaacttga gcagattaat aagccaaaaa cttgatgaag aagatccgat tgataagaga
    10021 tactttttag aagtgtcctc acccggggtt gagagacctt tgagaaaggt caaagactac
    10081 gagaggtttg caggagagga tattaagatc agcacctatc gaaaaataaa taattcgaaa
    10141 gaatttatcg gtaaattaat tggaattgat gaaaacaaag ttattacaat taaattgaaa
    10201 gatactgatg aagaaatgga gatcgactat tcagatgtat caaaagcaaa tttacatgag
    10261 gaatttaatt tttagtgtaa tttaaaggag gaaaaaaatg aatctggact tgctaaacgc
    10321 cttagatgac atcgagaagg acaaagggat atcaaaggaa gtgttacttg aagcaatcga
    10381 agtagcctta ctttcagctt ataaaaaaga ctttggttct aaagagaatg taagtgtaga
    10441 aattgatgaa gaatcgggag aagtgatggt cttctctcgc aagaaagtag tagaggaagt
    10501 aaaaaatgat cgttatgaga taccgcttga cgaagctgaa agaatcgatt catcgattga
    10561 agtaggagac tatttagatg tggaagtaac cccggagaac tttggaagaa ttgcagccca
    10621 aacggccaaa caggttgtca tgcagcgaat tagagaggcc gaaagagatg tactttatga
    10681 agaatataag cgcaaagagg gagaaattat caccgggact attcaacggt tccataaaga
    10741 caatgtatta attgatatgg gtaagaccga agcactactg cctgtatctg aacagatgcc
    10801 cagtgaggaa tatcgggttg gttcccgctt aaagctttat gtagtagagg tttcatctac
    10861 tactaaggga ccgcggattt tagtttccag aactcatccc gccttactga aaagattatt
    10921 tgaagtagag gtccccgaaa tctttgatgg cattgttgaa attaggtctg ttgcccgtga
    10981 ggcaggtttc aggtcaaaaa tagcggtgtc ttcttatgat gataaagtag atcccgttgg
    11041 ggcctgtgta gggccgcagg ggatgcgggt ccaatcagtg gtagaacaat taaacgggga
    11101 aaagatcgat atagtgcagt atagtgaaga tcccaaagaa tatgtttcta atgctttaaa
    11161 cccggccgaa gtaattgatg ttgtaaccga tgaagatcaa cagagcgcaa aagttatcgt
    11221 accagatttt caattgtccc tagcaatcgg aaaagaaggg caaaacgcaa gattagcggc
    11281 aaaattaacg ggttggaaaa tagatattaa aaaggaatca gaatttacag aagatgaaac
    11341 aaatgaagaa gaggaagaaa cagaacaaga atctaaagag gaaacagaaa gtttagattc
    11401 agcagagccc caggcagaag tagaacaaca accagaagat agccaagagg atgaaacaga
    11461 taaataagct aaaatttaat atggaggtgg ttagtttgag taaaatcaga gtatataagc
    11521 tggctgataa attaaatatg gaaagtaaag agttgataga tattttaaat aatctaggta
    11581 tagaagtatc cagtcatatg agtacaattg aagatgaaac ggctgatctt gtcaaagaag
    11641 ttgtaactga agaaagtaag agtgaagaag agattgaaga agaattagaa gatgagctgc
    11701 cagaggaaag cgcagaagaa caagaaactg aaccagaaat tgaagaggaa gaaattgaag
    11761 aagaagatga atccgactac attccggtaa cagaaggtat tacaatcaga gatttaggag
    11821 aaaaattaga tatttctgcc aataatatca tcaaagaaat gatgcagatg gggattatga
    11881 ccaatgttaa ttactcactt gatgaagatg aactagaaat tttaaaagaa gaacttggtt
    11941 tagagattaa attaaccgat gagatcgaag aggaagaatc ttctgaatta aaactcgaag
    12001 aaatagttga tagaccggaa gatctaaaac cgcgtcctcc catcgttact gtcatgggtc
    12061 atgttgacca cggaaaaact accttacttg atgtaattag aaagacccgc gtagcagaag
    12121 gagaagcggg cggaattact cagcacatag gtgcttatca ggctagttat gacgagaaaa
    12181 agataacctt tattgatacc cccggccatg aagcatttac cgcaatgcgg gcccgaggtg
    12241 ctcagttaac cgatattgtt atcttagtgg tagcagccga tgacggtgtt atgccccaga
    12301 ccgtggaagc tataaaccac gcccaggcgg ccggcattcc aataattgtt gccgttaata
    12361 agatcgataa ggcaaatgct caaccggacc gggttaaaca ggaattaaca gaacatagtc
    12421 tggttccgga agaatggggc ggtaatacga tctgtgtaaa tgtttcagct ttaaaaggtg
    12481 aaaatataga cgaattactg gaaatggtta accttgttgc agaagttgaa gaactaaaag
    12541 ccaatcccaa tagaattgcc gatggtgtca taattgaatc cgagcttgat agaggtcggg
    12601 gaccaattgc taccgtattg gttaaaaacg ggaccttaaa gatcggagat tatataattg
    12661 ccggttctat tacaggccga gttaaggcct tactggatga acacggtgaa agaatcgatg
    12721 aggctggacc cgcgacacct gttatagtgc ttggttttga tgacgtacca gaggcgggcg
    12781 attatgtaag agttatggaa gatgagaaac gggcccgtga tttagccgaa agaagacagg
    12841 aaaaagaaaa agaagaaagg ctcaaaaaag attctaaggt atccttagaa gacttatata
    12901 gaaatattca ggaaggtgaa attaaagaac tgaacgtaat aatcaaggct gatgtacagg
    12961 gttcaatcgc agctttagaa agttcactaa tgaatattga atcggatgag gtaagtgtca
    13021 atataatcca taccggggta ggagccatca atgaaacaga tgtaaatctg gccagtgctt
    13081 ctcaggccat aataattggt tttaatgtac gccctggtac taatgcccgc aaggcggctg
    13141 agaaagaaca ggtcgaaatt aaaacctata gagtaatcta taaagtaata gacgatatta
    13201 aaaatgcgat gtccggatta cttgaaccag aatataaaga gattgtggtc ggacgggtag
    13261 aagtaagaga cacctttaaa gtgcctgatg taggaataat cgccggtgct tatgttaaag
    13321 aaggtgaagt taatagaaat ga
//
LOCUS       DATGCJ010000137         9862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_61904, whole genome shotgun sequence.
ACCESSION   DATGCJ010000137 DATGCJ010000000
VERSION     DATGCJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61904"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..95)
                     /locus_tag="VKN64_06935"
     tRNA            complement(19..95)
                     /locus_tag="VKN64_06935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:Arg,seq:acg)
     gene            complement(260..1183)
                     /locus_tag="VKN64_06940"
     CDS             complement(260..1183)
                     /locus_tag="VKN64_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HMA60011.1"
                     /translation="MLSLFMAILCSSSIALIFKYSEDKRANRYLITSSNYLVASLISL
                     FLVMSSQNITWPSWSQFNLFVQELKMVFTNQQLKFTMESSNAWAVFIGIIGGIFFFLA
                     FIFYQKSVNEEGVGLAGTFAKLGILIPMILAIFIWNEIPATIQWFGIALALIAILIVN
                     LPLNKNWREALKWTLIGLFIFGGLGEFMNKFFQYYGRIYEKNLFLFFVFSTAFVISAA
                     YVMFKDYRIKPQEFMIGIFVGIPNLFSSFFLINALDQLKTAVVFPIFSAGSIVVISMG
                     GYFIFGETLNKREKTAIVLTIIALVMINIQK"
     gene            complement(1297..4836)
                     /gene="nifJ"
                     /locus_tag="VKN64_06945"
     CDS             complement(1297..4836)
                     /gene="nifJ"
                     /locus_tag="VKN64_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HMA60012.1"
                     /translation="MANKMKTMDGNTAAAHVGYAFTDVAAIYPITPSSPMAELVDEWS
                     AHGRENIFGQRVKLSEFQSEGGASGGVHGSLAAGALTTTFTASQGLLLMLPNMYKISG
                     ELLPGVFHVSSRSIATHALSIFGDHSDVMATRASGFAMLASGSVQEVMDLGGVAHLSA
                     IKSSLPFVHFFDGFRTSHEYQKIDVMDYDDLAELVDYDKVQEFRNNALNPEHPVTKGT
                     AMNPDIFFQVRETVNKYYERIPDIVEEYMQEITKITGREYHPFNYYGAEDAKHVIVAM
                     GSVTDTIEETVDYLVDQGEKVGVLKVHLYRPFSEKYFMKALPESVEKIAVLDRTKEPG
                     ALGEPLYEDVRSLFYDRDEDPVIVGGRYGLSSKDTTPTDIKAVFDNLKEEEPKNGFTI
                     AIEDDVTYTSLEPEEKIDSTPEGTVRCKFWGFGSDGTVGANKNAIKIIGDNTDMYAQA
                     YFSYDSKKSGGVTVSHLRFGEQPIKSTYLIDEADYVACHKDSYVNKFDMHTDLKPGGT
                     FVLNTNWSDDELDEKLPGGLKRYLAENDIDFYIINAVDIAQEVGLGNRINMVMQTAFF
                     KLAKILPVDEAIEYLKEAIEDTYGHKGEMIVDMNKKAVDKAMDALVKVDIPAEWAEAE
                     PEEAEEEDVPEFVEEIQRVVERQEGDSLPVSQFVGREDGHFPPGMSKYEKRGIAVNVP
                     EWQPDNCIQCNQCAISCPHAVIRPFLLDEEERDNAPEGMKTLKPMGKGMEGLEYRLQV
                     SPLDCTGCGVCAEVCPAPEKALVMEPFEDVLEAEKDNWEYVSDNVNYKEDVMDKYSVK
                     GSQFQQPLLEFHGACGGCGETAYAKLVTQLFGERMIIANATGCSSIWGGTAPTAVYTT
                     NDEGHGPAWANSLFEDNAEYGYGMYLGMEQFRDQLKMKMEEAMETDISDELKEAFQEW
                     IEGKDKAEASKQATEKMKPLLEEEKDNELVKFILDNKEFLVKKSQWIFGGDGWAYDIG
                     YGGLDHVLAQGDDVNVLVFDTEVYSNTGGQSSKATPTAAAAKFDAAGKRTKKKDLGRM
                     AMTYGYVYVAQIALGANMNQTVKAIKEAEAYPGPSLIIAYAPCIAHGLKNGMNCSVDE
                     EERAVKAGYWHLYRFNPELKEEGKNPFSLDSKKPEGDFEEFLLSEVRYAKLKQEFPEV
                     ADKLFDQAAQDAIERYQAYKRLEESLEPEGDEE"
     gene            5052..6185
                     /locus_tag="VKN64_06950"
     CDS             5052..6185
                     /locus_tag="VKN64_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMA60013.1"
                     /translation="MIYNKFGQKDWKTSILGLGCMRLPVIDGDNENIDEERSIEMIRY
                     AIDNGINYIDTAWPYHGGNSEGLVGKALQDGYREKVKLATKLYTPGIESVEDCNYYLN
                     EQLNRLQTDYIDLYLLHALNRKYWENYKSVDIIEWLEDMKAQGKIKNIGFSFHDDYDL
                     FEEIIDYYDWDFCQIQYNYLDTEFQAGKKGLKYAHDKGISVVIMEPLRGGTLAQTPPK
                     EVKDILDQSPNDRNAVEWALQWLWDQEEVDVVLSGMSNMEQLKENIELASQADQNTLN
                     NQERNLIAEARETYLDLFPVSCTGCNYCVPCPEGVSIPGIFSLYNEAEVYDKFAENQK
                     RYLNMDEASQADNCIECLQCEEACPQNLDITDHLKEAHAYFTE"
     gene            complement(6285..6626)
                     /locus_tag="VKN64_06955"
     CDS             complement(6285..6626)
                     /locus_tag="VKN64_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60014.1"
                     /translation="MRFEKDDSLSPDPRYDGENVDIGIFEGDEKLGIASVYFLDEITK
                     LAVSLYIEVDELDNAATLLDQIAEKYLDGDDRTKEKQRIKFHAPNYKEGLYYDRKNKE
                     FQERKLDYEWK"
     gene            complement(6747..7469)
                     /locus_tag="VKN64_06960"
     CDS             complement(6747..7469)
                     /locus_tag="VKN64_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMA60015.1"
                     /translation="MRKNKYYKIALFLLVGLLIFTQVGIAKEHEDVTKVNISHRIQRE
                     FKPEVANINVEVWTLDKELDTAYSKTTASMNEVIDILKTFENLTYSTTSFSVNQRYMD
                     QDNDKKRELYYEVSTMVKVETSNLDQLGNVIQQVIAKGATNIQGISYGLKNPEEAKNK
                     VIREGIKQIMNKAEVIKESLQKESYEIARLDINDNYSIYNYNYGVMRSEAKGAGTALP
                     VPEIAPQDVNINVNFSAEIWYK"
     gene            complement(7471..8208)
                     /locus_tag="VKN64_06965"
     CDS             complement(7471..8208)
                     /locus_tag="VKN64_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMA60016.1"
                     /translation="MKIAVFSDIHGNIEALNRVLEDIKREDVDKIYCLGDLVGYGPYP
                     NEVIDVIRDNEIETVMGNYDQGVGFDLDDCGCAYKTKAKQKLGDRSLAWTQREVTNEN
                     KEFLKSLKENIKFEAAGKKVLLVHGSPRKINQYLFFNHPAKSIKRMMDQYEADIMITG
                     HTHLPYIKKIEDKIIINDGSVGKQKPFNKEQETYSIEAKYIIVEVDEDSVNTELRSLP
                     YDYEKIAQAINDSELPDEFAEIIRGRG"
     gene            8378..>9862
                     /locus_tag="VKN64_06970"
     CDS             8378..>9862
                     /locus_tag="VKN64_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMA60017.1"
                     /translation="MKENKNTYTFKYFFNNYKNRYILGIVWLILVDILQLVIPWLLGD
                     FTDRLKTKSIDMRGIIYYALIIIGVAIFTAIFRYLWRLYIVGTSRVLEKKIREDFYSH
                     LLKLSTNYFNEQKTGDLMAHATNDIKAVRQAMGPGVVMLFDALFLTVATISILILRID
                     IRLTLIALIPLPFLALLSTYFSKSIHEKFTAVQEAFSNLTDKVQENFSGIRVIKAFTQ
                     EDLEIEKFASANKLNFDKNIELVKTWGLFHPLIQFITGLSFLIVIWYGGNLVIDGVIT
                     LGDFVAFNSYLGLLTWPMMAVGFVINLMQRGTASLKRLNNIFRAKEEIYDKDVKDIDD
                     IDGKIEFNNLSFQYNGSKEQVLKDINLEIKAGQTAAFLGRTGAGKTTIINLLLRLFNV
                     EKNEIYIDGHDINDLPIKLLRDKIGIVPQDNFLFSSTIAENIAFFDPDNIERSEIEKT
                     AKITEVYDNIMEFPAQFDTILGERGVTLSGGQKQRISMARAIIKK"
BASE COUNT         3047 a         1916 c         1592 g         3307 t
ORIGIN      
        1 tatgataagg ttaataaatg gcgtgcccgg aaggacttga accctcagcc tactgatccg
       61 tagtcagtcg ctctatccaa ttgagctacg ggcacatatt taactgcaaa cactattata
      121 catactataa taggtgttgt caagataatc aatctagctt tttaaaggta tttaatactt
      181 atatcctgtt cccttgcttt tatattataa catatccaaa gctgaattta cattgttcag
      241 catcagtttc ctttgataac tatttttgta tattgatcat tactaaggca ataatagtta
      301 gtacaatagc tgtcttttct ctcttgttta aagtctcacc aaatataaag taaccaccca
      361 tacttataac cacaatacta ccggcactaa agatcggaaa tactaccgca gttttaagtt
      421 gatccaaagc attgattaaa aagaatgatg aaaacaagtt aggaataccg acaaaaatac
      481 caatcatgaa ttcctggggt tttatcctat aatctttgaa cattacatag gctgcactta
      541 tcacaaaagc agtagagaat acaaaaaata agaagaggtt tttttcataa attcggccgt
      601 aatactggaa gaacttattc ataaactccc ctaaaccacc gaaaataaaa agtcctatta
      661 atgtccattt taaggcctct ctccaattct tatttaatgg taaattaaca attaaaatag
      721 caattagtgc taaggcaatc ccaaaccact gaattgtagc aggtatctca ttccagatga
      781 aaatagctaa aatcatcggt attaaaatac caagtttagc aaaggtaccg gctaaaccta
      841 ctccttcttc attaacactc ttttgataaa atataaaagc taaaaagaaa aatatacctc
      901 cgattatacc tataaaaact gcccaggcat tactggactc cattgtgaat ttaagctgtt
      961 gatttgtaaa taccattttc aattcctgaa caaaaagatt gaattgagac caagagggcc
     1021 acgtaatatt ttgtgatgac atcactaaaa acaaactaat taaagaagct accaaataat
     1081 ttgaagaagt aatcagataa cggttagccc ttttatcttc actatattta aatattaatg
     1141 ctattgatga actacataaa attgccataa ataaagataa caatatattc actcctagtt
     1201 aattaagact cactagatag tctatcataa atttaagtaa attaaaatgg aaaaagccgg
     1261 gtttgcaccc ggcttagtgt tttttactta ttatatttat tcttcatctc cttcaggctc
     1321 taaggattct tctaatctct tgtatgcttg atatctttcg attgcatcct gtgctgcctg
     1381 atcaaataac ttatcggcta cttctgggaa ttcttgtttt agcttagcat aacgtacttc
     1441 actgagtaag aattcttcaa aatcgccttc tggtttctta gaatctaagg agaatggatt
     1501 cttgccttct tctttaagtt ctggattgaa tctatataga tgccagtatc cagctttaac
     1561 agctctttct tcttcatcca cactacagtt catgccgttt ttcaatccat gtgcaataca
     1621 tggagcataa gctataatta aggatggtcc tggataggct tctgcttctt taattgcttt
     1681 tactgtttga ttcatattag cacccagggc aatctgtgct acgtatacat aaccataagt
     1741 catagccatt ctaccgaggt ctttcttttt ggttctctta ccagccgcat cgaacttagc
     1801 tgctgcagca gtaggagtag ctttggaaga ctgaccacca gtatttgaat agacctctgt
     1861 gtcaaatact aatacgttga catcatcgcc ttgagctaat acatggtcta gaccgccgta
     1921 gccaatatca taagcccagc cgtctccacc gaagatccat tgtgatttct taactaggaa
     1981 ttctttgtta tccaggataa acttaactaa ttcattatcc ttttcttctt ctagtaatgg
     2041 tttcatcttt tcggtggctt gcttggaagc ttccgcttta tctttacctt cgatccattc
     2101 ttggaaagct tcttttagtt catcagagat atcagtttcc atggcttctt ccatcttcat
     2161 cttcagttga tctctaaact gttccatacc gagatacatg ccgtatccat attcggcatt
     2221 atcttcgaat aaggagttag cccaggctgg accgtgtcct tcgtcatttg tggtataaac
     2281 agctgttgga gcagtaccgc cccagatgga agaacaaccc gttgcatttg caatgatcat
     2341 tctttctccg aatagctgtg ttactaattt agcataagca gtttctccac aaccgccaca
     2401 tgcaccgtgg aattcaagta atggctgttg gaattgacta cctttaactg agtatttatc
     2461 cataacatct tctttgtaat ttacattatc agatacatat tcccagttat ctttttcagc
     2521 ttcaagcaca tcttcaaatg gctccatgac taaggctttt tcaggtgctg gacaaacttc
     2581 agcacagaca ccacagccag tacagtcaag tggactaact tgcagtctat attctaatcc
     2641 ttccatacct ttacccattg gtttgagtgt tttcatacct tcaggtgcat tgtctctttc
     2701 ttcttcatcg agtaagaaag gtctaattac agcatgtgga catgaaattg cacattgatt
     2761 acattgaata caattatcag gctgccattc aggtacattt acggcaatac cgcgtttttc
     2821 atattttgac atacctggtg ggaagtgacc gtcttctcta ccaacaaatt gacttactgg
     2881 taggctgtct ccttcctgtc tttcaacaac tctctgaatc tcttcaacaa attcaggtac
     2941 atcttcttct tcagcttctt ctggttcagc ctctgcccat tctgcaggaa tatcaacttt
     3001 aactagggca tccattgcct tgtctacagc tttcttgttc atgtcaacta tcatttcacc
     3061 tttatgtcca taggtgtctt cgatagcttc ttttaagtat tcaattgctt catcaacagg
     3121 caagatcttt gccagtttga agaaagcagt ctgcataacc atgttgattc tattacccaa
     3181 tccaacttct tgagcgatat ctacagcatt gataatatag aaatcaatat cattttcggc
     3241 tagataacgc tttaagccgc cgggtaattt ttcatccaat tcatcgtctg accaatttgt
     3301 attcagaaca aatgtgccgc ctggtttgag gtcagtgtgc atatcgaatt tatttacata
     3361 cgaatcttta tgacaggcaa cataatccgc ttcatcgatt agataagtag atttaatggg
     3421 ctgttctcca aaacgtaagt gggaaacagt tacaccacca gattttttgg agtcatagga
     3481 gaaatatgcc tgtgcataca tatcggtgtt gtcaccgata attttgattg catttttgtt
     3541 agctccaaca gtaccatcag aaccaaagcc ccagaattta catctaactg taccttctgg
     3601 tgtggaatca attttttctt ccggttctaa ggatgtatat gtaacatcat cttctattgc
     3661 aattgtaaag ccattctttg gttcttcttc ttttaagttg tcaaatacag ctttgatatc
     3721 tgtaggagta gtatccttag aactgagtcc ataacgtcca ccaacgatta caggatcttc
     3781 atctctatca tagaataggg aacgtacgtc ttcataaagt ggttctccta aagcaccagg
     3841 ttcttttgtt ctgtctaata cagcaatctt ttctactgat tcaggtagtg ctttcataaa
     3901 gtatttttca gagaatggtc tgtaaagatg tactttaagt acaccaactt tctctccctg
     3961 atcaactaga tagtcaacag tttcttcaat tgtatctgtt acagagccca ttgcaacaat
     4021 tacatgtttt gcatcttctg caccatagta attgaatgga tggtactctc tacctgtaat
     4081 cttagtgatt tcctgcatat attcttcaac aatatcaggt attctttcat agtatttatt
     4141 aactgtctct cttacctgga agaaaatatc aggattcatt gcagtacctt tagttacagg
     4201 atgttcgggg tttaaagcgt tatttctaaa ttcttgaact ttatcatagt caactaattc
     4261 agctagatca tcataatcca tgacatcaat tttttgatat tcatgtgagg tacggaaacc
     4321 atcaaagaag tgaacaaatg gtaaacttga tttaatagct gataagtgtg ctactccacc
     4381 aagatccatt acttcctgaa cactaccaga ggctaacatt gcaaaaccgg aagctctagt
     4441 agccattaca tcagaatggt caccaaaaat agatagagca tgtgtcgcga tactacgtga
     4501 acttacgtga aatactccag gtagtagttc tccagaaatc ttatacatat ttggaagcat
     4561 caataatagg ccctgagaag cagtaaaggt agttgttaaa gcacccgctg ctaatgagcc
     4621 gtgaactccg cctgaagctc caccttctga ttgaaattca gataatttaa ctctctgtcc
     4681 gaaaatgttt tcccgtccgt gcgcactcca ttcatctact aattctgcca ttggggatga
     4741 aggagtaatt ggatagatcg cagcaacgtc tgtaaaagca taaccaacat gcgccgcagc
     4801 agtgtttcca tccatcgtct tcatcttgtt tgccatcttc tttccccctt tatattagtt
     4861 aagtgaaata tatacttatt ccaattctat tttagtatat tatactaaat ttgctgtgtc
     4921 aaattttatc aaactattac aatctaatga taattgtgcc ctacttactg ggcttttttg
     4981 gacttgatag aattaatatg ctatactatt atatgtatga aaataaacaa ttagcaaagg
     5041 ggaatcaaat tatgatatat aacaaatttg gtcaaaaaga ttggaaaaca tctatacttg
     5101 gtttgggctg tatgcgctta cctgttatag atggagataa tgaaaatatt gatgaagaaa
     5161 gatctatcga aatgattaga tatgccatcg ataatggtat aaactacatc gatactgcct
     5221 ggccatacca cggtggtaat agcgaaggtt tagtaggaaa agcacttcag gatggttacc
     5281 gggaaaaagt aaaattggca actaaacttt acactcccgg tattgaaagt gtagaagact
     5341 gcaactacta tttaaatgaa caattaaacc gactgcaaac cgactacata gacctgtact
     5401 tgctgcacgc tttaaataga aagtactggg aaaattataa aagtgttgat attattgaat
     5461 ggttagaaga tatgaaagca cagggtaaaa taaaaaatat cggtttctcc ttccatgacg
     5521 actatgacct ttttgaagaa atcatagact attatgactg ggatttctgc caaattcagt
     5581 ataattatct agatacagaa tttcaggctg gtaaaaaagg tctaaagtat gcacatgaca
     5641 aaggaatttc ggttgtcatc atggaaccat tgagaggcgg aacattagca caaacccctc
     5701 cgaaggaagt aaaagatata ttagaccaaa gtcctaacga tagaaatgct gtggagtggg
     5761 ctttacaatg gctctgggat caagaagaag ttgatgtggt tttaagtggt atgagcaaca
     5821 tggaacagtt aaaagaaaat atcgaacttg cttcacaagc agaccaaaat actttaaata
     5881 atcaggaaag aaacctaatt gcagaggcac gggaaacata tctagactta ttccctgttt
     5941 cctgtacagg ctgtaattat tgtgtgccct gtccagaagg agtttctata cccggtatct
     6001 tctctcttta taatgaagca gaagtttatg ataaatttgc agaaaaccaa aaaagatatt
     6061 taaatatgga tgaagcatcc caggcagata actgtattga atgcttgcaa tgtgaagaag
     6121 cctgtccaca aaatttagat attaccgatc atctaaaaga agcgcatgct tatttcacag
     6181 aatagaatat taaagtaaat tatagtaatt aataaaataa atgggaagct aggaccaata
     6241 agtcttagct tcccattttt atactttatt atattaaatt ttaattattt ccattcataa
     6301 tccagcttgc gttcctgaaa ttctttattt ttacgatcat aatatagtcc ttctttataa
     6361 ttaggggcat ggaatttaat cctctgcttt tctttagtgc ggtcatctcc atctaaatat
     6421 ttttccgcta tttgatctaa gagagtggca gcattgtcca gttcatccac ctctatatac
     6481 aacgaaacag caagtttagt aatctcatct aaaaaataca ccgaagctat tcctagtttt
     6541 tcatctcctt caaagatccc gatatctacg ttctctccat catacctggg atcaggcgat
     6601 agagaatcat ccttttcaaa cctcatgaat ttatacctcc aattagattt attaatgtta
     6661 attaaattta ttacaaaaaa ctcttgtcct tttaataaat aagggactct atactacaca
     6721 tttgccttat tagtttaacc ctctttctat ttataccata tttcggcact aaagttaaca
     6781 ttaatattga catcctgtgg tgcaatctcc ggcactggta aagctgttcc ggctcctttt
     6841 gcttcacttc tcattactcc gtaattataa ttatagatac tatagttatc attaatatct
     6901 aacctggcaa tctcataact ttctttttgc aggctttctt taataacttc cgctttgttc
     6961 attatctgtt taattccttc tcggattact ttatttttag cttcttccgg gtttttcaaa
     7021 ccgtaactta ttccctgaat attagtagcc cctttggcaa taacctgttg aataacattg
     7081 ccaagttgat ctaaattact tgtctcgact ttaaccatag ttgatacttc atagtatagt
     7141 tcgcgttttt tatcattatc ttggtccata taacgctgat taacactaaa agaggtagta
     7201 gaataagtaa ggttttcaaa tgtttttaaa atatcaataa cctcattcat actggcggtc
     7261 gttttggaat aggctgtatc cagttcttta tctaaagtcc agacctctac atttatattg
     7321 gccacctctg gtttaaattc tctttggatg cgatggctaa tattcacctt ggtaacatct
     7381 tcatgttctt tcgctattcc cacctgggta aagattaaca aaccaaccag gagaaataaa
     7441 gcaattttat aatatttatt ctttctcaaa ttaacccctc cctcttatta tctccgcaaa
     7501 ctcgtccggt agttcactgt cattgatagc ctgggcaatc ttctcataat cataaggcaa
     7561 acttctaagt tcagtattaa cagagtcttc atcaacctca actattatat atttcgcttc
     7621 tatactataa gtctcctgct ctttgttaaa aggtttttgt ttacctacac tcccatcatt
     7681 tattattatc ttatcttcaa tctttttaat ataaggtaga tgagtgtgac ctgtgatcat
     7741 gatatctgcc tcatactggt ccatcatcct tttaatactt ttagccggat gattaaaaaa
     7801 taggtactga ttaattttgc ggggactgcc gtgtacaagt agtacttttt taccggctgc
     7861 ttcaaactta atattttctt ttaaactttt taaaaactct ttattttcat tagtaacttc
     7921 tctttgggtc caggccagtg atctatcacc gagtttttgt ttagcttttg ttttataggc
     7981 acaaccacag tcatcaagat caaaaccaac tccctgatca tagttgccca taaccgtttc
     8041 aatttcatta tctctaatga catcaattac ttcattggga taaggtccat aaccaaccag
     8101 gtcacccaaa cagtatattt tatctacatc ttctcttttt atatcttcca gcactctgtt
     8161 taatgcttcg atattaccat gtatatcaga aaaaactgct atcttcattt tattatgcct
     8221 cctgtcaatt atatgttcta tctttaataa tacattattt ataataacat attcaaatac
     8281 tcgccttaat tttaacataa atttaatata attagaggag aacaacttct ttcatctaat
     8341 ttattataag taaattaact tttttggagt gtaaactatg aaagaaaata aaaatactta
     8401 tacatttaaa tatttcttta ataactataa aaataggtat attttaggga tagtctggtt
     8461 aattttagtt gatatcctgc agcttgtcat cccctggctt ttaggcgatt tcaccgatcg
     8521 gttaaaaact aagtctatag atatgagggg tattatctac tatgctttaa ttatcatcgg
     8581 tgttgcaatc tttaccgcca tctttagata tctctggcgg ctttatatag taggtacttc
     8641 ccgggtgctc gaaaaaaaga tcagagaaga tttttatagt cacctcttga aactctctac
     8701 taattacttt aatgaacaaa agacaggtga tctaatggcc catgccacca atgatatcaa
     8761 agccgtaagg caggctatgg gaccgggggt tgtgatgctt tttgatgccc tctttttaac
     8821 agtagcaact atctccattt tgatattgag aatagatatt agattaacct taatagcatt
     8881 aataccatta ccgtttttag ccttactatc aacttatttc agtaaatcaa tccatgagaa
     8941 gtttactgcg gttcaggaag ccttttctaa tcttaccgat aaggtgcagg aaaacttctc
     9001 tgggattaga gtaataaagg cctttactca ggaagattta gaaattgaaa aatttgccag
     9061 tgccaataag ttaaactttg ataaaaacat cgaactggtt aaaacctggg ggcttttcca
     9121 tcccttaatt cagttcataa ctggtctaag cttcttgatt gtcatctggt atgggggcaa
     9181 cttggtaatc gatggagtaa taaccttagg tgattttgtc gcctttaata gttatctagg
     9241 gcttctaaca tggccgatga tggcagtcgg ctttgtaatt aatctaatgc agagaggtac
     9301 agcctccctc aaaagattaa ataatatatt tagagccaaa gaagaaattt atgataaaga
     9361 tgttaaagat atagatgata ttgatggtaa aatcgaattt aacaaccttt cctttcaata
     9421 taatggaagt aaagaacagg tcttaaaaga tattaatctt gaaattaagg ccggccaaac
     9481 tgctgctttt ctgggtagaa ccggggccgg taaaaccacg attattaatt tattattaag
     9541 actatttaat gtagagaaaa atgagattta tattgacggt catgatatta atgatcttcc
     9601 cataaaatta ttgagagaca agattggtat tgtacctcaa gacaatttct tgttttccag
     9661 tactatcgct gagaacatcg ccttctttga ccccgataat atagagcgtt cagaaattga
     9721 aaaaacagct aaaataacag aagtttatga taatattatg gaatttcccg cccagttcga
     9781 taccatcttg ggagaaaggg gagttaccct ttccggtggt caaaagcaga gaatatcaat
     9841 ggccagggct attattaaaa aa
//
LOCUS       DATGCJ010000138         7634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_597277, whole genome shotgun sequence.
ACCESSION   DATGCJ010000138 DATGCJ010000000
VERSION     DATGCJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597277"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VKN64_06975"
     CDS             <1..1347
                     /locus_tag="VKN64_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HMA60018.1"
                     /translation="KAQKGKISLKDDIRKQLDNLADEIGNRADSLLILEGDKNVASVH
                     NKKAFELREKIYQQRRNRVNSIVEEIITKEEKNSSIGDKIGSMLLRPITGIPILLVIL
                     YALYQFIGVFVAQTLVGITEEMIMIETYQPFIINLVSNFIAPESLIGKILIGEFGVLT
                     MTVTYILGLLLPLVAGFYFFLAILEDSGYMPRIAALVDRGLNYLGLNGRAIIPMLLGF
                     GCVTMATVTTRLLGSKREKIIAIFLLGLAIPCSAQLGVIAGLIAPLGLDYFIIYVMTI
                     FAVYAVTGTFLNKVLPGESTDLLIDLPPIRVPEIKNVLQKTYLKSKYFIFEAGPIFAL
                     GAVGITLMKEFRILDSIKELVAPLVVGWLQLPKDATEAFIMGIIRRDFGAAGLSSMAL
                     TNDQTLVALIVLTLFVPCIAAMLVMIKEQGIKQAAMVWVGSWVAAFTVGGLVALFI"
     gene            1359..1508
                     /locus_tag="VKN64_06980"
     CDS             1359..1508
                     /locus_tag="VKN64_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60019.1"
                     /translation="MQCTTDGKRICPTCGNEIEDPDIMKCPRCNSLLILKCSECKKCS
                     FFKKL"
     gene            1691..2224
                     /locus_tag="VKN64_06985"
     CDS             1691..2224
                     /locus_tag="VKN64_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HMA60020.1"
                     /translation="MNLSTRKIVISGMLGAISIILGATGLGLIPVPTPAGHATIMHVP
                     AIIGGVLEGPVVGFFIGLIFGIFSFIRATNPIFADPLIAVVPRLFIGVGAYYIYYLLK
                     KYNVSFALILAGIIGTLINTIFVLGLAVLRGYLPFKAAASVAFLHGIPEVILAAVIVV
                     IVGKAILSIQKPSGAKN"
     gene            2311..3093
                     /locus_tag="VKN64_06990"
     CDS             2311..3093
                     /locus_tag="VKN64_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMA60021.1"
                     /translation="MSIMTIGDCVIDYYPELDQEFIGGSGLNTAVHLKEKDLKVDLIG
                     VIGDDIKGRKILDYLKHKNFDLTKIKVRNGDTALAYIKKDGNDFKIETVQEGIKRNYS
                     LSKEDIRVVKNYDIVHTNIYSHTIHLLPYLKEKNDLVSFDYSFKVDYEKLEKIKEYID
                     ILFISKDNMLKEEFSQLKEYNIDYVVLSSGNKGFIVLHNEQEYFKEPVGDYIKDSTGA
                     GDTLIAEILFGIHQKQELDTILDRAARKAYQSCLGIGPFHSF"
     gene            3201..4871
                     /gene="hcp"
                     /locus_tag="VKN64_06995"
                     /gene_synonym="priS"
     CDS             3201..4871
                     /gene="hcp"
                     /locus_tag="VKN64_06995"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HMA60022.1"
                     /translation="MSMFCYQCQEAAGNEGCTVRGVCGKTEDVANLQDLLIFVLKGIS
                     YYAQKAKEYGVNVDQQTGPFIMKNLFSTITNGNFDAARFEENIQEALEMRESVKNEFM
                     KAYKNETGQEFDEEIPEAATWSSDNVEDFYKKGEEVGVLSTKNEDIRSLRELITYGVK
                     GIAAYADHAYILNESDLELFDFMQKALKATLDDSKSVEDYINLVMETGDYAVKTMALL
                     DKANTENYGHPEPTEVNVGVNDNPAILISGHDLKDMEELLKQTEGTGVDVYTHGEMLP
                     ANSYPAFKKYDHFVGNYGNSWWLQKEEFEKFNGPVLLTTNCLVPPKDSYKDRVYTTGV
                     VGFDGVTHIEGRPEGGQKDFSEIIDHAKQCDSPTELETGTIPGGFAHEAVMNVADKVI
                     EAVENGDIERFVVMAGCDGRHNTREYYTDVAKELPEEAVILTAGCAKFRYNKLDLGDI
                     GGIPRILDAGQCNDSYSLVYIAQQLQEAMGVDDINDLPISYDIAWYEQKAVTVLLALL
                     SLGIKGIRLGPTLPAFLSENVVKVLVENFDIKPIGEVKEDVEKIMAGK"
     gene            complement(4967..6337)
                     /locus_tag="VKN64_07000"
     CDS             complement(4967..6337)
                     /locus_tag="VKN64_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMA60023.1"
                     /translation="MFKTKYFLKNSCPTILPTMIILLSLSVMVGLFIFAFSCPVQAVQ
                     KNNKINYPAAKFAVITDPHLFEVEGNINNPVYLKYGVEDRKLLSLSPEILEIAVDKII
                     QEGGIEFVLLPGDLTDSGDIVSHKVVAGILQKLKQNGINVYVIPGNHDGFNTEDFQNT
                     SIPREIVTPENFINIYNNYGYKEAIFKDENSLSYVVEPIKGLWLLMMDSCIYNSPDSY
                     HVCNGRIRKPTKDWINDILIKADEQNKAVIGSLHHSLLEHYEGHDRFFSKYIVDDFEE
                     LSAQFAARNMKVVFTGHHHAQDIVKKEFENNSFIYDIETSSLIGFPNAYRIVEINQNN
                     EVSITSKYIKEVPSFEGDFPTHTKNYVLGRVKEVAADKLDPFHINPVDKDKISDNAAE
                     AMIAHYQGNEVAPESLNIEGINIWSRFIYSFYRRLLASLYDDPAPDDLNIKIDLNTGE
                     WTNLDN"
     gene            complement(6401..6955)
                     /locus_tag="VKN64_07005"
     CDS             complement(6401..6955)
                     /locus_tag="VKN64_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60024.1"
                     /translation="MDEKDLIADRFVSLLQEHNLENISLAMVNGKMDMEVGNYFDDKY
                     EILDYILRDKLEEKIALINSLKVKEMTLQNKLILFFDYHFDFLKEYPNISEILIKTSM
                     IEDDKRLEIIKSYMRNFKSTFSELIEAEIKKNKVRDVDPSIIASAILHATHGVTVRIK
                     YDETYDYAKAKKELINFIYLGLNN"
     gene            complement(7031..>7634)
                     /locus_tag="VKN64_07010"
     CDS             complement(7031..>7634)
                     /locus_tag="VKN64_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMA60025.1"
                     /translation="STLKKYKKDEIIFFEGEKGEYIYIIKKGQVKMLKMNQDGDEQIL
                     NVFKKNDILAEVIVFDKENYPATAIALNNVELIAIDSNKLSKIFLDHPQITVKIMKVM
                     SERLRRAQQNIRDFGLKDSKSRLASLLYHLAEKHGKESGKNEISISLFLTQQELANMI
                     GTTRETVSRTLNNIEKEDIITTSRKKIVINDMKGLKKLMN"
BASE COUNT         2461 a         1219 c         1445 g         2509 t
ORIGIN      
        1 aaggctcaaa aaggtaagat tagtttaaaa gatgatatta gaaagcagtt agataactta
       61 gccgatgaaa taggtaatag agctgacagc cttttaatct tagaaggaga taaaaatgta
      121 gcatcagttc ataataaaaa agcttttgag ctcagagaaa agatctacca gcaaaggcgt
      181 aatcgcgtta atagcatcgt tgaagagatc attactaaag aagaaaaaaa tagcagtatc
      241 ggtgataaga ttggttccat gttattgaga ccgatcaccg gtatacctat tttattagtt
      301 attttatatg cgctttatca gtttattggt gtctttgtag ctcagacctt agttggtatt
      361 acagaagaaa tgataatgat agaaacctat caacctttta taataaattt agtttctaac
      421 tttattgcac cggagagtct tatcggtaag attttaatag gagagttcgg tgtactaacg
      481 atgacggtaa cctatatttt gggattgtta ttaccactgg tagccggatt ctatttcttt
      541 ttagctatct tagaggattc cggttacatg ccgaggatag ctgctttagt tgacagaggt
      601 ttaaattatt taggtctaaa tggtagggca attataccga tgcttttagg atttggttgt
      661 gtaacaatgg ctactgttac cacacgcctc ttgggttcta aaagggaaaa gataattgct
      721 atctttttat taggtttagc gattccttgt tccgcacaac tgggagttat agccggactg
      781 atagcacctt taggcctaga ttactttatt atatatgtaa tgaccatttt tgctgtctat
      841 gcagtgaccg gtacattttt aaacaaggta ttacccggtg aatcaaccga cctattgatt
      901 gatttacccc ctattagagt gccggaaata aaaaatgtac tacagaagac atacttaaag
      961 tccaaatact ttatatttga agccggaccg atttttgctc tgggagcagt tggtattacc
     1021 ttgatgaaag agtttagaat actggacagt attaaagagt tagtagctcc cctagtagtg
     1081 ggttggctgc agctgcccaa ggatgcaaca gaagcattta tcatgggtat tatcagaaga
     1141 gattttgggg cagccggatt aagctcgatg gccttaacta atgatcagac tttagttgct
     1201 ttaatagtat taactctttt tgtgccctgt attgcagcca tgttagtgat gattaaagaa
     1261 cagggtatta aacaggcggc catggtctgg gtaggtagtt gggtggcagc ctttaccgtc
     1321 ggtggactag tcgcgttatt tatctaagga ggtaatttat gcagtgtaca actgatggaa
     1381 agaggatctg tcccacctgt gggaatgaaa ttgaagatcc cgatataatg aaatgtccaa
     1441 ggtgtaacag ccttttaatc ttaaaatgtt cagaatgtaa aaagtgcagt ttcttcaaga
     1501 aattataaca aaaaaagaag gttagaattg gtactctttt tgcttgaata aattaaatta
     1561 gtataatata ataacctcag atgacatccg taatggagtc atactattta agtaataaaa
     1621 agcagaggat gtcaaaaaaa tattactacc tggatccaag agaaggaccg ggataggagg
     1681 aattgcagaa atgaatttat ccacacggaa gatagtaatt tcaggcatgt taggcgctat
     1741 ctcaattatc ttaggtgcga caggacttgg actaattccc gtgcctactc cggccggcca
     1801 tgcaactata atgcatgtac cagctatcat cggaggagta ctggaaggac ctgtagttgg
     1861 attttttata ggcttgatct ttggtatttt cagctttatc agagcgacga acccaatctt
     1921 tgctgatcct ttaatcgctg ttgtacccag attatttatt ggagtaggag cctattatat
     1981 ctattattta ttaaagaagt ataatgtaag ttttgcccta attttagcag gtataattgg
     2041 tactttaatc aatactatct ttgtattggg attagcagtt ttaagaggtt acttaccctt
     2101 taaagcagca gcttcggttg cttttttaca cggcataccc gaggtaatat tagctgctgt
     2161 tatcgtagtg attgtgggta aggctatttt aagtattcaa aaaccttctg gggcaaaaaa
     2221 ttaatattta aacatattcc taaaatattc attatcagac tactaaaagc ccttgcaaaa
     2281 gggctttttt tgttaggagt tgagtttaaa atgagtatca tgacaattgg agactgtgtt
     2341 atagattatt atcccgagct agaccaggag tttataggcg gcagtggttt aaatacggct
     2401 gttcacctga aagagaaaga tttaaaagtt gatttaattg gtgtaatcgg agatgatatt
     2461 aagggaagga aaatccttga ttatttaaaa cataaaaact ttgatctaac taagatcaaa
     2521 gtacgaaatg gagatactgc actcgcttat attaaaaaag atggtaatga tttcaagata
     2581 gaaacggtgc aggaaggcat taaaaggaat tatagcttaa gtaaggaaga tataagagta
     2641 gttaaaaatt atgatattgt acataccaac atatattctc ataccattca tttattaccc
     2701 taccttaaag aaaaaaatga tttagtgagt tttgactatt cttttaaagt tgattatgaa
     2761 aaattagaaa agattaaaga atatattgat atccttttta taagtaaaga taatatgttg
     2821 aaagaagaat tttcccaact taaagaatac aacattgact atgttgtatt aagctcgggt
     2881 aataagggtt ttattgtatt acataatgaa caggagtatt ttaaagaacc ggtcggtgat
     2941 tatattaaag acagtacggg agccggggac actttgattg ctgaaattct ttttggcatt
     3001 catcaaaaac aagagctaga tacaatatta gatcgggctg cccggaaggc ctatcagtct
     3061 tgtttaggta ttggtccatt tcattctttt taaattaatt ttaagctgtg tgatataaat
     3121 catagaacta taaatatatt ctccatataa ttgttaatgt aatgaataaa tgaggaaaaa
     3181 taaaaaggag gattatttat atgtcaatgt tttgttatca atgtcaggaa gcggccggaa
     3241 atgaaggctg tacagtcaga ggggtttgtg gaaagaccga agatgtagct aacctacagg
     3301 acttacttat ttttgtactt aaaggcattt catactatgc tcaaaaagcc aaagaatatg
     3361 gtgtaaatgt tgaccagcag accggtccct ttatcatgaa aaaccttttc tcaacaatta
     3421 ccaatggtaa ttttgatgcc gcaagatttg aagaaaacat ccaagaagca cttgagatga
     3481 gagaaagtgt aaaaaatgaa ttcatgaaag cctataaaaa tgaaaccgga caggagttcg
     3541 acgaagagat accagaagca gcaacctggt ccagtgataa tgtagaagac ttctataaga
     3601 aaggggaaga agtcggagta ttaagcacta aaaatgaaga cataaggtct ttaagagaat
     3661 taataacata tggtgtaaaa ggaatcgcag cttatgctga ccacgcctat atccttaatg
     3721 aaagtgattt agagctgttt gatttcatgc agaaggcctt gaaagctact ttagatgaca
     3781 gtaaatctgt tgaagattat ataaatctag taatggaaac cggggactat gcagttaaga
     3841 ccatggcttt attagataaa gctaatactg aaaattacgg tcacccggaa ccaactgaag
     3901 taaacgtggg agttaatgat aatcccgcta tcttaatcag cggccatgat ctaaaagata
     3961 tggaagaact cttaaaacag accgaaggta caggggtaga tgtctatacc cacggtgaaa
     4021 tgttaccggc taattcttat cccgccttta aaaagtatga ccactttgtc ggaaactatg
     4081 gtaattcttg gtggctgcaa aaagaagagt ttgagaagtt caatggccca gttctcttaa
     4141 ctaccaactg tctagtacca cccaaagatt cctataaaga cagagtttat acaaccggtg
     4201 tagttggatt tgacggtgta actcacattg aaggacgccc cgaaggtgga cagaaagatt
     4261 tctccgaaat aatcgaccac gctaaacaat gtgattcacc gacagagtta gaaaccggaa
     4321 ctattcccgg cggctttgcc catgaagctg taatgaatgt tgcagataag gtaattgaag
     4381 ctgtagaaaa tggagatatt gaaagatttg tagttatggc cggttgtgac gggcgacata
     4441 atacaagaga gtattatacc gatgtagcaa aagaactccc agaagaagca gttattttaa
     4501 ctgccggctg tgccaagttc aggtacaaca aacttgacct aggagatatc ggcggtattc
     4561 caagaatcct tgatgccggt cagtgtaatg attcttattc cttagtctat attgcccagc
     4621 agctgcagga ggcaatgggt gttgatgata taaatgatct tcccatttca tatgatatag
     4681 cctggtatga acaaaaagcg gtaacagtgc tcttagcgct cttatcctta ggcattaaag
     4741 gtattcgttt aggaccaacc ctgcctgctt tcctctcaga aaatgtagtt aaagtattgg
     4801 ttgaaaactt tgatatcaaa ccaatcggtg aagtcaaaga agatgttgag aagataatgg
     4861 caggcaaata ataaatattt cagtatagat cataataaag aaagccttag tccactcaac
     4921 tgtgggctaa ggcttttttt tgagttaaaa agtaaaatat atattattaa ttgtctaaat
     4981 tagtccattc cccagtattt aaatctatct ttatatttaa atcgtcagga gccgggtcat
     5041 cataaagaga agctaataat cttctataaa aactgtatat aaagcgcgac cagatattta
     5101 taccctcaat attaagagat tccggtgcca cttcattacc ctgataatgg gctatcattg
     5161 cctcagcagc attatctgag attttatctt tatcaacagg atttatatga aatggatcta
     5221 atttatctgc agcaacttct ttaactcttc ccagcacata atttttggta tgagttggaa
     5281 aatcaccttc aaaagaaggc acctctttaa tatatttact ggtaatactc acttcattgt
     5341 tttggtttat ttcaactatt cgataagcat taggaaaacc aattaaagaa gaagtttcaa
     5401 tgtcatagat aaaagaatta ttttcaaatt cttttttgac tatatcctga gcgtgatgat
     5461 gaccggtaaa tactactttc atattacggg cagcaaattg agcagataat tcctcaaaat
     5521 cgtcaactat gtatttagag aagaaacggt catgaccctc ataatgttct aataagctgt
     5581 gatgaagtga acctatgaca gctttatttt gctcgtctgc ttttattaaa atgtcgttga
     5641 tccaatcttt tgtgggtttt cttattcttc cgttacaaac atggtaactg tcaggactat
     5701 tatagataca actgtccatc attaacagcc ataacccttt aatcggttct accacataac
     5761 ttaaggagtt ttcatcttta aatattgctt ccttatagcc gtagttatta tagatattta
     5821 taaaattttc gggtgttact atctcacggg gaatacttgt attttgaaaa tcttcagtat
     5881 taaagccgtc gtggttgccg gggataacat agacattgat tccgttttgt ttaagctttt
     5941 gtaaaatacc agcaacaact ttatggctta ctatatcacc ggaatctgta agatcgcccg
     6001 gtagtaaaac aaattctata ccaccttctt gtattatttt atcgaccgct atttccaata
     6061 tttcgggact tagacttaaa agtttcctat cttctactcc atatttgaga tatacggggt
     6121 tgtttatatt tccttcaact tcaaagaggt gggggtctgt tattacagcg aacttagcgg
     6181 caggatagtt tattttatta tttttttgta cagcttggac cgggcaagaa aaagcgaaaa
     6241 taaaaagacc aaccatgacg cttaaagata atagtattat catagttggt aggatagtgg
     6301 gacaagaatt ttttaagaag tatttagttt taaacatatt taggacctcc taattaataa
     6361 tcgataaagt attagaaagc cgattaaaat tgagctgaaa ttaattgttc aaacccaggt
     6421 agataaagtt tataagttct ttttttgcct tagcgtaatc ataagtttcg tcatatttta
     6481 ttcggacagt taccccatga gttgcatgta agattgcact tgcaattatt gaagggtcaa
     6541 catctctaac tttatttttc tttatttcag cttcaattag ttcactgaat gttgatttga
     6601 agtttctcat gtaggatttt attatttcta atcttttatc atcttcaatc attgatgtct
     6661 tgatcagtat ttcgctgata ttgggatatt cttttaaaaa gtcaaaatgg taatcaaaaa
     6721 acaaaattaa tttattttgt aaagtcatct cttttacttt tagactattg atcaaagcta
     6781 ttttttcttc tagtttatcg cggagtatat aatctaaaat ttcatattta tcatcaaaat
     6841 aatttccgac ttccatgtcc atttttccat taaccatggc gagactaata ttttccaaat
     6901 tgtgttcttg caataaagaa acgaacctgt ctgcaatcag atctttttca tccatttttc
     6961 acacctctaa tattgattgt tacatctatt ataatattta atctaagaaa ttaatatgaa
     7021 ttatatcaca ctagttcatc agttttttga gtcctttcat atcatttatt acaatttttt
     7081 tgcgggaagt agttatgata tcctcctttt ctatattatt taaggttcta gaaaccgttt
     7141 cgcgggtagt tccgatcata ttggccaatt cctgttgggt taaaaagagt gagatagaaa
     7201 tctcattttt tccactttct ttaccgtgtt tttcagccaa atggtaaagt aagctggcca
     7261 gcctgctttt actgtcctta aggccgaagt ccctaatgtt ctgttgggct cttcttaacc
     7321 gttcactcat tactttcatt attttgactg taatctgggg atggtctaaa aatattttag
     7381 ataatttatt actatcaatg gcaataagtt caacattatt taaggcaatg gcggtagccg
     7441 ggtaattctc tttatcaaag acaataactt ctgctaagat atcatttttc ttaaagacat
     7501 ttaaaatttg ttcatccccg tcttgattca tcttgagcat ctttacttgc ccttttttaa
     7561 ttatataaat atattctcct ttttcacctt caaagaaaat tatttcatct tttttatatt
     7621 tctttaatgt ggaa
//
LOCUS       DATGCJ010000139         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_419656, whole genome shotgun sequence.
ACCESSION   DATGCJ010000139 DATGCJ010000000
VERSION     DATGCJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419656"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="VKN64_07015"
     CDS             <1..1562
                     /locus_tag="VKN64_07015"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HMA60026.1"
                     /translation="RKGFVITLFSLILVTVMSVASFACTDIVISKGASADGSVITSHT
                     VDGRYDSRIQIIPAQDHEEGTMAPVYENIVYADRMDLKKLGEIPQVPHTYKYFHGAYP
                     YANEHQVIIGETTLGGARETKNSEEAIMTIEQLEIFALQRAKTAREAIQIMGSLAEEY
                     GYRESCWLGECLTVTDPNEAWVFEIFGVGPLWTKDSGKSGAVWAAKRVPDGHVTVVPN
                     FSRIQKMEPNSDDMMFSDNYEEVAIELGLYDPEEDGEFVWNIAYGDVEGGESNRLWRF
                     YNLMQPSKDWAYDDVANYPFSIEPEEKVSVHDVIEMFRDTQEGTVNDMADADAWYYED
                     DGERVKSVLANPQVNSDWRNLLDIDYYRPIARYYCSYYFVSQARDWLPDEVGGVIWFG
                     LDNPENSPFIPMYVGTESVPESWSTLNRDKLDRDSAWWAFALIDDTVNRRYGDLKPKL
                     DEVLMPMQNHMFEMQEMIEQQAFDYLMDGRNEKAKEFLTNYTSSLMNWTEQRYWNLAD
                     EFMFELNNNRH"
     gene            1795..3432
                     /locus_tag="VKN64_07020"
     CDS             1795..3432
                     /locus_tag="VKN64_07020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HMA60027.1"
                     /translation="MKMKKSLIITVFSILLVFVLSAATFACTNVVVGKDASTDGSVIT
                     SHTVDGQYDARVKVIHGKEYEEGAMMELKKNICHAGIPGYEVSVVGEIPQAEETYTYF
                     HAGYPFMNEHSVIIGETTFFGRGEYSNENAMIMIEQLEILGLQRAKTAREAIQVMGSL
                     AEKYGYADIGETLTVTDPNEAWVFEIIGPGPLWTPGSDQPGAVWAAQRVPDGHVTVAA
                     NRSRIEELDLEDSDNYMASSNVKSFAEEMGYWDPDEGPFVFWKAYCPDPYGAPYYQAR
                     REWRALSMLAPSQDFEPNLPKDEHYPFSIEPDEKVSVRDVMTILRDYLEGTQFDLTEG
                     MAAGPFGTPNRYATPGSVKPEDRKDADWERAISMFRCSYSFVSQARDWLPEPIGGVAW
                     FGEDAPHTTCYIPIYCGVTDLPESYETGARDKFDESAWWAFNFVSNWADLKYDYMIED
                     IRKEQDKFEKEFLAMQPTIDKAAEELYNKDPELARQFLTDYTNSNLVKVEDRWWELAE
                     ELVYKYHDGYVNTEDDVKSVGYPTWWLEEVNFGDTNR"
     gene            3634..4209
                     /locus_tag="VKN64_07025"
     CDS             3634..4209
                     /locus_tag="VKN64_07025"
                     /inference="COORDINATES: protein motif:HMM:NF040376.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HMA60028.1"
                     /translation="MFNSRVKSVFMVMMLLILVFSMSVQGQEVTAEGPIIATTCGQSP
                     GALMVTQICKQVGLECEQQDLLEGDYLKENDFKTVIITMGTSGKGMGAAGTDMKAEVE
                     RINGIIEVANEKGMTIIGAHIEGEARRVDDNDAKSIEVVAPYSHIIIVREDSNKDGYF
                     TDLGEEKDIPVYVIEKTLGLTEPLKEIFQVE"
     gene            4281..5570
                     /locus_tag="VKN64_07030"
     CDS             4281..5570
                     /locus_tag="VKN64_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HMA60029.1"
                     /translation="MYTHAGIILAVIAAVFFIARYFKVTTELSIFAAALAGGIVHTGG
                     IPVRHIVEGTFTYFDICLIFITATVFINFLKEAGGISFMVRSIIKTFHERRFLCLLFL
                     TIILLIPGALTGAGAATVLTVGTLVGTVLTYMGISKTRTTAIIFVCATMSAAAPPVNL
                     WAMMTAAGANMPYVGFMQPLLVLSVAGALITMFTLGGKGTPVDLDKALAELPEPPEDM
                     NWYRVLLPFGVLFALILAGRIWPFNMPILGLPLMFVISALIVLITTPRRLPILDIIGH
                     TIRSLIPLVGIMIVVGSLIQVMANSGARGLVSLGVVTLPLWFLFISLWAILPIAQGVF
                     QYAVAPLLGVPLIFLFNMKGMDPIIALSAMAVMWPVGDGFPPTAVVGRAAVMELKFEG
                     RYYRDFLKTASVPMLIILAICTLFLVFSNQLSFLVGG"
     gene            5766..>5985
                     /locus_tag="VKN64_07035"
     CDS             5766..>5985
                     /locus_tag="VKN64_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HMA60030.1"
                     /translation="MSDKKIRIIKYSTLALVIVAMIISGLKFYNHRNYKEPVILGPGV
                     TKVETIGDYYEPVKGTVNDCNIYILDSGK"
BASE COUNT         1955 a          863 c         1271 g         1896 t
ORIGIN      
        1 taagaaaagg ttttgttatt actttattct cactaatatt agtaacggtt atgtctgtag
       61 cttcctttgc ttgtactgat attgttataa gtaaaggtgc ttctgctgat ggatcagtta
      121 taacctctca cactgttgat ggcaggtatg attctcgtat acaaattatt cccgcgcaag
      181 atcatgaaga aggtacaatg gcacctgttt atgaaaacat agtttatgca gatcgaatgg
      241 atttaaaaaa attaggagaa atacctcaag tgcctcacac ctataagtat tttcacggag
      301 catatccata tgcaaacgag catcaggtaa taattggtga aacaacttta ggtggtgcta
      361 gagagactaa aaatagtgaa gaagctataa tgacaattga acagcttgaa atttttgctt
      421 tacagagagc caaaacagcc agagaagcta tccaaattat gggtagtttg gctgaagagt
      481 atggttatcg tgaatcttgt tggttgggag aatgtttgac agttactgat cccaatgaag
      541 catgggtatt tgaaatcttt ggtgttggac cactatggac taaagatagc ggtaaatctg
      601 gtgcagtatg ggccgccaaa agagttccgg atggccatgt aactgtagta ccaaacttta
      661 gtcgtattca aaaaatggaa cctaattcag atgatatgat gtttagtgat aactatgaag
      721 aggttgccat cgaattaggt ctttatgacc ctgaagaaga tggagagttt gtctggaata
      781 tagcttatgg agatgttgaa ggtggagaaa gcaataggct atggagattc tataatctaa
      841 tgcagccttc taaagattgg gcttatgatg atgttgcaaa ttatccattt tctatagaac
      901 cggaagaaaa agtatctgtg catgatgtaa ttgaaatgtt ccgagataca caagaaggaa
      961 ctgtaaatga catggcagac gctgatgcct ggtattatga agatgacggt gaaagagtta
     1021 aaagtgtatt agctaatcct caagttaata gtgattggag aaatctttta gacatagatt
     1081 attacagacc aattgcccgt tactactgtt catattactt tgtgtctcaa gcaagagatt
     1141 ggctacctga tgaggtagga ggagttattt ggttcgggct tgataatcca gaaaactcac
     1201 catttattcc aatgtatgta ggtacagaaa gtgtacctga atcctggtct acacttaata
     1261 gagacaaatt ggatagagat tcagcctggt gggcatttgc attaattgat gatactgtaa
     1321 accgtcgtta tggtgattta aaaccgaaac ttgatgaagt attaatgcca atgcagaatc
     1381 acatgtttga aatgcaagag atgatagaac aacaagcctt tgattatcta atggatggaa
     1441 gaaatgaaaa agctaaagaa ttcttaacaa actatacaag ttctcttatg aactggactg
     1501 aacaacgcta ctggaattta gcagatgaat tcatgttcga attaaataat aacagacatt
     1561 aaaattacta ggttatatta ctaaatgggg gataaccggt taaaaccggt tatcctttgc
     1621 aataattatt aattttattt aagagaaata tgagatttaa taggattggg gagttcaatg
     1681 aataaagtat aaagtagtga aaatgttcaa attaaaaata ttctcggggg tgattactta
     1741 aataatctta atttgaatat tttctaatag atttttaagg gggacaatca agaaatgaag
     1801 atgaaaaaaa gtttaattat tacagttttt tcaatcttat tagtttttgt cttatcggca
     1861 gctacttttg cctgtacaaa tgttgttgta ggtaaagatg cttctactga tggatctgta
     1921 attacctcac acacagttga cggtcagtat gatgccagag ttaaagtaat acatggtaaa
     1981 gaatatgaag aaggcgctat gatggaatta aagaaaaaca tctgtcacgc tggaattcct
     2041 ggttatgaag tgtctgttgt aggtgaaata ccacaagcag aagaaactta tacttacttc
     2101 catgctggat atcccttcat gaatgaacac tctgtaatca ttggggaaac aactttcttt
     2161 ggtcggggag aatattctaa tgaaaatgcc atgattatga tagagcagtt agaaattcta
     2221 ggacttcaga gagcaaaaac cgcccgtgaa gcaattcaag taatgggtag tttagctgaa
     2281 aaatatggat atgctgacat tggtgaaact ttgacagtta cagatcccaa tgaagcttgg
     2341 gtatttgaaa taattggtcc aggaccatta tggacacctg gcagtgatca acctggagca
     2401 gtgtgggcag ctcagagagt gcctgatgga catgtaaccg ttgctgcaaa tagaagtaga
     2461 attgaggaat tagacttaga agactctgat aattatatgg cttccagtaa tgttaaatcc
     2521 tttgcagaag aaatgggcta ttgggatcca gatgaaggac cttttgtttt ctggaaagca
     2581 tactgtccag atccttatgg agctccttat tatcaagcta gaagagagtg gagggcttta
     2641 agtatgttag ctccttctca agactttgag cctaatttac ctaaggatga acattatcca
     2701 ttttcaatag aacctgatga aaaagtttct gttagagatg taatgactat cttaagggac
     2761 tatctggaag gtacccaatt tgatttaaca gaaggaatgg ctgcaggtcc ttttggaact
     2821 ccaaatagat atgctactcc tggtagtgta aaaccagaag atagaaaaga tgcagattgg
     2881 gaaagagcca tttcaatgtt tagatgttct tacagttttg ttagtcaggc tagagactgg
     2941 ttaccagagc caattggtgg agttgcctgg ttcggtgaag atgcaccaca tacaacttgc
     3001 tatatcccaa tttactgtgg tgtaacagat ctaccggaat cctatgaaac tggtgccaga
     3061 gataaatttg atgagtcagc ctggtgggca tttaacttcg tatccaattg ggcagacctt
     3121 aaatatgatt acatgatcga agatatcaga aaagaacagg ataaatttga aaaagaattc
     3181 ctggctatgc agcctactat tgataaagca gcggaagaat tatataacaa agatcctgaa
     3241 ttagccagac agttcttaac agactatacc aatagtaatc ttgtaaaagt agaagacaga
     3301 tggtgggagt tagcagaaga attagtatac aaatatcacg atggatatgt taataccgaa
     3361 gatgatgtaa aatctgttgg atatcctacc tggtggttag aagaagttaa ctttggtgac
     3421 acaaatagat agtaaaaaga taactggggt gaaccgggta ttaacccggt ccccctctgg
     3481 aattaatatc tatttaatat aatttttata acattattat atttagaatt aataaactgt
     3541 agcataaaat tgttatataa acaaatatag ataaaaatct atacttaatt tagttacagt
     3601 aatataatat aaaatcaaga aggagggttt gtaatgttta acagccgagt aaagagtgtt
     3661 tttatggtca tgatgttatt aatactagtc ttttcaatgt cagttcaggg ccaagaagta
     3721 actgctgaag gtcctattat agcaactacc tgtggtcaaa gtcctggtgc tttaatggta
     3781 actcaaatat gtaaacaggt tggtctagaa tgcgaacaac aggacctatt agaaggtgat
     3841 tatctaaaag aaaatgattt taagactgtt attattacaa tgggaaccag tggtaaagga
     3901 atgggagccg ctggtacaga tatgaaagca gaagttgaaa ggatcaatgg cataatagaa
     3961 gttgctaatg aaaagggtat gacaataatc ggggctcata ttgaaggtga agctcgtaga
     4021 gttgacgaca atgatgcgaa atcgatagaa gtagttgctc cttattcaca cattataatt
     4081 gtaagagaag acagtaataa agatggttat tttactgatc taggcgaaga aaaagatatt
     4141 cccgtttatg ttatagaaaa aacattagga ctaaccgagc ctttaaaaga aatatttcag
     4201 gtagaataat gatttgttga tgatgagccc ggttgattta ttaaccgggc tatattcctg
     4261 aaaaagggag ggaaatatag atgtatacac atgcaggtat aatattagca gtaattgctg
     4321 ccgtcttttt tattgcccgc tattttaaag ttacaactga actttctatc tttgctgcgg
     4381 ctttagcagg agggatagtt cacacgggcg gaatcccggt cagacatatt gtggagggta
     4441 cgtttactta tttcgatatt tgtttaatct ttattacagc tacagtattt attaattttt
     4501 taaaagaagc cggcggaatt agttttatgg ttagaagtat aataaagact tttcatgaaa
     4561 gaagattttt atgtttacta tttttaacaa ttatcttatt aataccaggt gctttaacag
     4621 gagcgggagc agcaactgta ttgactgttg gtacgttagt aggtacagta cttacatata
     4681 tgggaattag taaaacaagg actaccgcaa tcatctttgt ctgtgctact atgagtgcag
     4741 cagcaccacc tgttaacctt tgggcaatga tgacagctgc tggggcaaat atgccttatg
     4801 ttggtttcat gcagccgtta ttagtactat cagtggcagg agctttaata acaatgttta
     4861 cattgggagg aaaaggtact cccgttgatt tagataaggc attagctgaa ctgcctgaac
     4921 cacctgaaga tatgaactgg tatcgggttt tattaccttt tggtgtttta tttgctttaa
     4981 ttttagcagg cagaatttgg ccttttaata tgccaatttt aggactacct ttaatgtttg
     5041 ttatttctgc attaattgta ttgataacaa ctccacgtag actacctatt ttagatatca
     5101 ttggtcacac catccgttca ttaattcctc ttgtaggtat tatgatcgta gtaggaagtt
     5161 taattcaggt aatggcaaat agtggagcac gcggtctggt ttccttaggt gtagttacct
     5221 taccactttg gtttctattt ataagtttat gggctatttt acctatagct caaggtgtct
     5281 tccaatatgc tgtcgcccct ttattgggtg tacctttgat ctttttattc aatatgaaag
     5341 ggatggatcc cattattgca ctttccgcta tggctgtaat gtggccggta ggtgatggtt
     5401 ttccaccgac cgcagtggta ggaagggcag cagttatgga attaaaattt gaaggcagat
     5461 attataggga ctttttaaaa accgcgtctg taccaatgtt aataatactc gctatttgca
     5521 cattattttt agtatttagt aatcagcttt cattcttggt aggtggttaa agaaaggagt
     5581 ttaattatgt taactttatt aaatactatt gtaaattata gttattatat tttgacgatc
     5641 ttcatttcat ttgttttaat tagagaaatt attaaaacta aagatgtaca ggaatcaatt
     5701 ctctactgta taatcttaat tccatttgtt ttacggtcgt tacacattaa gtagggggtg
     5761 agttcatgtc agataaaaaa ataagaatta ttaaatattc tactttagcc cttgttattg
     5821 ttgcaatgat tatttcaggt cttaaatttt ataatcatag gaactataaa gaaccagtta
     5881 ttttaggtcc aggggtgact aaagttgaga ctattggaga ctattatgaa cctgttaaag
     5941 gaactgttaa tgattgcaat atatatatat tagatagtgg taaac
//
LOCUS       DATGCJ010000140        20187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_463716, whole genome shotgun sequence.
ACCESSION   DATGCJ010000140 DATGCJ010000000
VERSION     DATGCJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 20187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20187
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463716"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VKN64_07040"
     CDS             <1..369
                     /locus_tag="VKN64_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMA60031.1"
                     /translation="KFKHTLESIPGIGPVLSAGILAEITDINLFENDSKLAKYAGLTW
                     LHKQSNQYKSEETHMNKSGNKYLRYYFIQAAESARKNCDEYRDYYNKKYRESTKHKHR
                     RALVLSARKLVCSAAQKYAL"
     gene            complement(690..2366)
                     /locus_tag="VKN64_07045"
     CDS             complement(690..2366)
                     /locus_tag="VKN64_07045"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HMA60032.1"
                     /translation="MKHILKRYSLIKDYKNRPITIILIAFIFFLSASLVLYTGGTKHS
                     YLHIIYIPILLSAYFYKGWGGFIAGIIAGIILGPFMPINTANMAMQEAMNWSFRMFFF
                     VLIGTIAGFLFDLLEHQLNKINRIAYFNESTNLPNKTKFRKDIEQKIEKGEEFHVIVF
                     SIKNYPDIYKLVGSRNFTDFIQLMIEHITDYEGMGQQLYYINDSKYSMWIKKQSKKDL
                     IKKLRKFNLHLNSPVEFKNISIFTDIIMGISTYPEDGSGFDELLEKGFLALEKVKEKK
                     LNFWIYEKEAVEIKYNNIELLGDLNKSLEEDHFELYYQPKIDLRNKEATTFEALIRWN
                     HPEKGFIPPGEFIPKVEKSSLIEPLTDWVISRSISDIKEYEYKEEELDVNIAINISAR
                     NFQDPNFVDNLFRHIDQFELDPSKYAIEITETDLMIEMESNIEKLYRLRSRGVQIYLD
                     DFGKGYSSLKYLKKLPIDYIKIDKFFIEDIEKEMTKQDIVLSIIKLSHALDIEVVAEG
                     VEKEEQLRSLQNMGCDYAQGYYFTKPDKKEKIIKWAKEFNNTTITYSELE"
     gene            complement(2470..3798)
                     /locus_tag="VKN64_07050"
     CDS             complement(2470..3798)
                     /locus_tag="VKN64_07050"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HMA60033.1"
                     /translation="MIQGKSIEQWQEEYPLLKKIINTDEVFWLNPKYQKDPNNGVLEM
                     TLSDIKEAEARLKRFAPYIQEVFPETKESNGIIESALEGIDDFKSELVDLFNTDMPGQ
                     LYIKRDDNLPIAGSIKARGGIYEVLKYAETLALKNNIIDKDDNYKQFASKKFKDLFKE
                     YSLAVGSTGNLGLSIGITGATLGFKTTVHMSSDAREWKKQLLRSKGVIVKEYDSDYSK
                     AVEEGRKESEKDPKSYFIDDEKSKDLFLGYAVAALRLKEQFEKEQIKIDDNHPLFVYL
                     PCGVGGGPGGICFGLKKVFGPNVHCFFAEPTHSPAMLIGMMTELHNKITVQEFGIDNI
                     TVADGLAVGKPSKFVGKLMENLLTGIYTVQDDILSKLLKRLYDKKKIKLEPSALAGFP
                     GLAKVYNNISDIELLELSKRGKTVNNSTHLIWATGGSMVPEPIFREYLDQ"
     gene            3867..4097
                     /locus_tag="VKN64_07055"
     CDS             3867..4097
                     /locus_tag="VKN64_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HMA60034.1"
                     /translation="MNSQFNWGKFLIYIGLIIAFIGFLINTIEINFNWFGNLPGDIRI
                     ERENFKLLIPITSLLIITIILNLIIYIIRLFM"
     gene            complement(4143..4433)
                     /locus_tag="VKN64_07060"
     CDS             complement(4143..4433)
                     /locus_tag="VKN64_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60035.1"
                     /translation="MNKNLVSNMRYVLSKAKNAHHKYEQNVLDGRFDKEWYKWYADFI
                     LNSNVRNELRKNIEEEEMIQFLKDITDDYTEKEVEEDWEDYAAEKLVDNFSA"
     gene            complement(4489..5652)
                     /locus_tag="VKN64_07065"
     CDS             complement(4489..5652)
                     /locus_tag="VKN64_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60036.1"
                     /translation="MKKVTIVIPTYWTWPSDQDNKKEVYNFDHPTPLDGKGTLGRLLF
                     SLKNLEYKNFDVLIISSAAHDKLKEKVEAKVDLIVDQYKNDLPIKHFSYSDLPDLKEK
                     IKNKIEDKFLEEINLDNYPNIRNIQLLVPYMQGTEIVIGIDDDEIIEDNMYLEKAVQY
                     AGERREGDYIAGVAGYYTDIKGSQYLQENKNPDNLFEKKNKLMNDTYKKLESTDKRLV
                     RTPFILGGNMIIPSKTIERIPFDPYNSRGEDIDYLINAGLEGYNFYFDKRLKVVHLPP
                     SYGKKQTEVAKLKSDIYRFLYEKKKIEIAKDKKGFKKLDIEELSPYPGEFLTGSIEKA
                     ASNMLCKTFKDLHIENPRAKTENYIGKAVDKADKLAPTYFNFNKSWKDLLSSL"
     gene            complement(5669..5923)
                     /locus_tag="VKN64_07070"
     CDS             complement(5669..5923)
                     /locus_tag="VKN64_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60037.1"
                     /translation="MGLIARELEENGIATVTLNMFLELAEKVLPPRTVNVNFPFGAPF
                     GDPDNKKLQLKVIKASLDMLDSCNTPGEINELPYNWRESN"
     gene            complement(5942..6421)
                     /locus_tag="VKN64_07075"
     CDS             complement(5942..6421)
                     /locus_tag="VKN64_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HMA60038.1"
                     /translation="MDLKKIVEFMREEVDPDFDFEDNRDKEVPFNKLDKKINTANIVL
                     ISSGGFHLKNDEPFDTDDPMGDPSYRMIPKDTEPELIKISHTHYDHKYVNKDLNCAFP
                     LQLLKELENKKEIGAVADENYSFMGYCLKIKELKANAVELAKKLKNKDIDAALISPT"
     gene            complement(6434..7474)
                     /locus_tag="VKN64_07080"
     CDS             complement(6434..7474)
                     /locus_tag="VKN64_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMA60039.1"
                     /translation="MYRGLIADKVDSLKLKDFKESDLKANEVKVRVKYGSPKHGTEMT
                     MLKGVSPFQKEYYDNDWDMFLKKDKKEEVFPMDLGNMWVGLIVEKGKAVKNLKIGQRV
                     AGYGGLKEYQYINKEDVLVLPENMNWKSALCYDPARFALGGIRDSSLKLGDRVAVFGL
                     GAIGLLTVQMALKAGAVWVAAVDPIEKRRKAADRLGADLVLDPNQTDVGYEIKQNTNK
                     KGVDHSIDTSGHTPVLQTAIRSTAYGGNVTTVGWYKELKGQLNLGREAHFNIPEIIFS
                     RACSEPNREHPRWDLERINKTCWQMLSRGHYDCRDIITPVVDFKDILESYRYYVDKNP
                     AESIKLGVKFNQ"
     gene            complement(7486..8271)
                     /locus_tag="VKN64_07085"
     CDS             complement(7486..8271)
                     /locus_tag="VKN64_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HMA60040.1"
                     /translation="MKIACQDTLIPGDNIKKILLNTKELGFDGIEISGEKFLQNPTEV
                     NEAFDSTDIELAIICAGHRGWLLSEDKQKQREAVRDIKELLNYAAKYKAIGVLTPTIY
                     GTSDYLPYPPRKRSLKEDEKLLIDTLNELGAQAVQSGVYLLLEPLIRYQTHYINKLSE
                     GIEIIDKLDNNGVKLMADTFHMNMEEKDIDKSLKEAKDYLYHIHLSDSNRRLPGQGHI
                     DFPRHLATLKSIGYNKYLSFEALKPDNPTKQLKESLKYILKNI"
     gene            complement(8645..8989)
                     /locus_tag="VKN64_07090"
     CDS             complement(8645..8989)
                     /locus_tag="VKN64_07090"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HMA60041.1"
                     /translation="MNIEGYQSMRSNLIKNQSEDNQQIENIRNFNNDGEADEDEEKLK
                     QAITDFSAVFLKLMFKSMRSTLPDNKYIDGGYAEEVFTDMMDKQISELGSGQSTFKNL
                     NRAMFEQLDQTK"
     gene            complement(9007..10140)
                     /locus_tag="VKN64_07095"
     CDS             complement(9007..10140)
                     /locus_tag="VKN64_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HMA60042.1"
                     /translation="MKLKYKKSLLILTVLLILPMVLTLNIQAQNQDPIVKIGNITRIK
                     GIRDNQLIGYGIVVGLAGTGDSNRSQATVQSVANMMGEFSINVTPDQIRSKNLAAVML
                     TADLKPFVHSGDRIDVTVSSMGDADSLQGGTLVMSPLKAANGQIYAVAQGPVSIGGFN
                     VQSGGSSARKNHPTVGRIPNGALVEREIEFDLNKDHINLLLQNPNFKTADNIAKALNK
                     KLEYDSIARASDAGTVRVDVPDEFEGRVVDFIAQVNNIDVRSNIEAKIVIDEKTGTIV
                     LSHNVRISTVAVAHGNISVTIKTKEKVSQPPSFSEGETKKTEETEIEVSEEEGNIVMI
                     SNENTIEDLVSALNTIGATPRDIVSIIQKIKAAGALHAKLELI"
     gene            complement(10160..10744)
                     /locus_tag="VKN64_07100"
     CDS             complement(10160..10744)
                     /locus_tag="VKN64_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HMA60043.1"
                     /translation="MKKLLTIMMIPIIMFTLTTALLAEGNSLWSEESGDIYKDRKEHV
                     KGDIITVLIKEDANAIQSANTSTSQASEVDAEAGFGIFSFLKAFGFGYSDSGSADGQT
                     SRSGTLEADITTQIVEIKENGNLKISGTKNININGEKQSIKLTGVIRPADISLENTIS
                     SKMIANADIEYEGEGPVGDKQKTGFLEKLVNWIF"
     gene            complement(10759..11733)
                     /gene="flgA"
                     /locus_tag="VKN64_07105"
     CDS             complement(10759..11733)
                     /gene="flgA"
                     /locus_tag="VKN64_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HMA60044.1"
                     /translation="MKNKRSILALTIMLIISITVPLSAFEVIIEKEVRIKEESIKLGK
                     IAEFNEITAAEEKMINEIDLGKAPLPGYKKYINKALVKLMLKKQGFKTDEFRLNFPEQ
                     IIVRREAKQIEAEKIKEFLKKKLNSILRDNSDKFTIEVNLRREKITIPKQAYSLEILE
                     NRDIEPGQMTIPVAINIAGKEYKRFYIPVNIKVYKEAYVAKKYISQGTKLKRDDFVYK
                     MVEVDSIEDEKFVKKGSNIFTQNIELSYSLKQGDILKKNNYNTPYLINWGDRVQAKII
                     VGDVELTLMVVARERGKQGQYINVENPKNRYKFQAKVISPQLVELIKD"
     gene            complement(11760..12542)
                     /gene="flgG"
                     /locus_tag="VKN64_07110"
     CDS             complement(11760..12542)
                     /gene="flgG"
                     /locus_tag="VKN64_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HMA60045.1"
                     /translation="MIGALWTAATGMNTQQTSIDVTSNNLANVNTGGFKKSRVNFADL
                     MYRQIRQPGTPNAQGAQIPVGIEVGHGSKVASTQKIFTEGNIRNTDNPLDLLVEGDGF
                     FQVMKPDGTVAYTKNGSFKRDSTGRVVTADGYPLQPEIVIPEDATSISITSDGTVNIK
                     TGNTTEQIGQIQLYRFANPAGLNSLGRNLFQQTVASGDPQTGVPGEQGFGAISQGFLE
                     MSNVKVVEEMVNMISAQRAYDVNSKAIKAADEMLQTANQLRR"
     gene            complement(12636..13388)
                     /gene="flgF"
                     /locus_tag="VKN64_07115"
     CDS             complement(12636..13388)
                     /gene="flgF"
                     /locus_tag="VKN64_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HMA60046.1"
                     /translation="MIKGLYTAASAMQTNMRRMDITSNNLANADTDGFKKSNSVQRTF
                     SEMLISRIDSNGQTEIGALGSGVDIAENYTDYSHGKIEHTNNKLDIALKGDGFFTVQT
                     PQGIRYSRNGNFTLNSQNQIINEQGHLLLNENSEPIQTIPGREISIDKSGQIYLGELE
                     GERINIVDFADYRNLSKVGDNLYQTADGIEGMPADEVQVMQGYLESSNVNIVQEMAGM
                     IQTTRAYETNQKVIQTIDNSLDKAVNDVGRLG"
     gene            complement(13385..13687)
                     /locus_tag="VKN64_07120"
     CDS             complement(13385..13687)
                     /locus_tag="VKN64_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60047.1"
                     /translation="MAWLVILMAINVFYNNTRLNYNDKVIASLNKRKEKQLIAKREFT
                     EKHKDNIEKLNKKHKKALDEFNQKKSIDKIAKNLKMGIRETRLIAKLYSYRGGEML"
     gene            complement(13703..14005)
                     /locus_tag="VKN64_07125"
     CDS             complement(13703..14005)
                     /locus_tag="VKN64_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60048.1"
                     /translation="MSDPINPNTLIPMSLEVEKIEQMKQVHSANEQQTLNQAQKEKDK
                     KKQKTVNKKEKSENLTIDKDGANQNKRKQEQKEEDQDESKENKNKKIKGSVIDVKV"
     gene            complement(14019..15677)
                     /locus_tag="VKN64_07130"
     CDS             complement(14019..15677)
                     /locus_tag="VKN64_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HMA60049.1"
                     /translation="MDSLVVKAASKEKAAQKAYQKLSKERKIKLRDLKVEEKDGMFSG
                     LRSKKRYQITLHPKKDINFNLLRNALEIDKKLALDIGKVVKTNLSTYQKNEEAELIIK
                     KSADKMKAFVDYIPPQGLGEDFTEEDIKAKLKANDIVYGLKDYGINLILESEAPLEDI
                     VIAEGKAAQPGEDARLEFHFDRSGKNVGTVQEDGSIDFHDLGIVNNVEAGEKLVSKIE
                     ATEGEEGKNIEGKTITPEKPEDVKLPKGENTEITEDNTLVSTEVGHISYKNDKVNILT
                     VYKVQGDVDFNTGNIDFNGSVFIEGNVTEGFEVKAEGDIHVRGNVEGAHLKCSGDITI
                     NKNFIAKNKGEIECEGNLTTKTVQNGTVNCKGDVFVKDAIMHSKIHAAGSIELTGHKG
                     LIVGGEVRATKEVKANIIGSSLATKTIVSAGIDPHTRSRCNKAKENLTECKSNYLKTV
                     KAINILNQMKEKYGQLPEEKANMYDRLIETKSDLKEKINKNEKTVNELSEKINKATQG
                     EVMVKRKVYPGVKVQIGKYHHEVDEVHTRTVFKVEEGELRRFGL"
     gene            complement(15706..16455)
                     /locus_tag="VKN64_07135"
     CDS             complement(15706..16455)
                     /locus_tag="VKN64_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HMA60050.1"
                     /translation="MLSNQELWRRFKEENDQKAREQLIMEYLDLVKYQAGRVNTYVPD
                     RIELEDLESYGIIGLIDAIEKFDPSRGYKFNSYAQKRIKGEIIDQLRKLDWLPHSLRQ
                     KAKVIKSKTRELESKMNRSPKINEIAAELDFSKDQITKVLKKLEESQWVSIYSELNGK
                     SLIDWLPSSKVNKPQKILDKKNAIKILKNAIVNLSDKQKLVVSLIYYEELTQKEVAEI
                     MDLSPARISQIHKKAVYRLRGYLSRKKEEFQ"
     gene            complement(16479..16898)
                     /locus_tag="VKN64_07140"
     CDS             complement(16479..16898)
                     /locus_tag="VKN64_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60051.1"
                     /translation="MKLKLTGSTFAALIVYMLTALIGLIQFNPLSSVLISGVKFALTA
                     FVFTFLITYAMEIMNKKTQATNQDSSDDNKQKQPQGKSSREYEKTKASQNQANAQEES
                     TGGEQQKEDKDNEENNWDFEDDFSEMEPPVIEYEKNN"
     gene            complement(16900..17559)
                     /locus_tag="VKN64_07145"
     CDS             complement(16900..17559)
                     /locus_tag="VKN64_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMA60052.1"
                     /translation="MSNPDKNLSINQNIEVVISEGPYQGSYNSKVSDLLEEKIKVMAI
                     YKREELIPLRKGVEVQLLYEGDSAFYSLTTEILGREKYPIPTLILKRTNEVNRIQRRR
                     YFRLQVNKKIKYKSLKDIEDEKKEAEFKKTTAVDLSGGGLLMVLRDDLNLKEKLVLNL
                     NIENLDKLVKGRIVRIKHNQDGYSKTAGIEFIEMERKDRDKIISFLFNYQRKLRRRGM
                     I"
     gene            complement(17568..18467)
                     /locus_tag="VKN64_07150"
     CDS             complement(17568..18467)
                     /locus_tag="VKN64_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HMA60053.1"
                     /translation="MNNQAAKLKDYMESNNIKDFDEIGNRDKDKQSRIIAVASGKGGV
                     GKSTITVNLALALRELEKEVFLIDSDIGMANIDVLLGLKTKYDLNHVLKGLCSLKQAV
                     ETGPYGLNVLSGISGIDDVLNISNENIKHLLSGVTEIERNYDVILLDTGAGASQSVIN
                     FTTAADEILIVLTPEPTSIMDSYSLIKLLSSSQYRGKLHLVVNRVDSQKEADSIIDRF
                     ITTARKYLDLNIDLLGTIPNDNLISKSIKKQHPVFKLYPESEVNDYFLQLAGNLIGKE
                     LDLPSRGIKNFFYKLIGQIAPGR"
     gene            complement(18460..19512)
                     /locus_tag="VKN64_07155"
     CDS             complement(18460..19512)
                     /locus_tag="VKN64_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMA60054.1"
                     /translation="MKVKKYIGDNIQDTIFKVKVDLGSDAIILDTRKFTKGGFLGFFG
                     QEKVEVLAGLEEPRKAEENTQKEINHLKEMIQDLSNNVNEKKVKEETPEVLTTFQQYL
                     RLQNVREENVKAITDYLSEDSNLDENNLKLKSKNHLEDLLGEPVPIKAAEETQVITFV
                     GPTGVGKTTTIAKIAAEFAIKEGKKVHLITADTYRIAAVQQLRTYSDIINIPLKVVYD
                     GDEFKKAVKECRKEADLILVDTPGRNWKNKWELGQLNEFTKDSLVDEIHLMVSLNMSS
                     RDLEFAVDNFSRLKPDKMILTKLDETSTYGDVINISQKYDMPFSYITFGQDVPEDIDK
                     ANSEILYNYLMGDFNE"
     gene            complement(19513..>20187)
                     /locus_tag="VKN64_07160"
     CDS             complement(19513..>20187)
                     /locus_tag="VKN64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HMA60055.1"
                     /translation="EAEMANYTIVDPPSVMATHLTELIKEHAHELIGRQEVKDLVDNV
                     EENNSAVVNELLPDLLSLGEIQKVLQNLLWENIPVNNLVTILETLADYARTTKNIDLL
                     TEYVRQGLSRQITNQFVDQNNQLVVITLDPQLEENLGQAVEQSDQGNYLAVEPQQAQQ
                     LIESISNEAQKLLQKGKEPILVTSPMIRRPLKELTSRSFPQLTILSFSELTPNVNLEV
                     LGTVVN"
BASE COUNT         5538 a         4143 c         2924 g         7582 t
ORIGIN      
        1 aaatttaaac atactcttga atccattcca ggtattggtc ctgttctttc tgctggtata
       61 cttgctgaaa ttactgatat taatcttttt gaaaacgatt ctaaacttgc taaatatgca
      121 ggcctcacct ggcttcataa acaatctaat caatacaaaa gcgaagaaac tcatatgaat
      181 aaatctggta acaaatacct tcgttattat tttattcaag ctgcagaatc tgcaagaaaa
      241 aattgtgatg aatatcggga ttattacaac aaaaaataca gagaatctac caaacacaaa
      301 caccgtagag cactggtttt aagtgctaga aagttagttt gttctgctgc gcaaaaatac
      361 gctttatgac ccttctagat atcttaaaaa ccgcggaggt ggttgctgac ttaataatgt
      421 tttaagactt ttagaaatca gtctataaag aatttctcta aagataattg ttttatcttt
      481 tcacccagga tagagttaat tacctttagt aatttaaatt ttcttaaccc ttttaaaaat
      541 catatttaaa ggggatgggt gaactatgcc ctaaaaaggg cgtcaaatga aatagaagat
      601 ttacttgaca tgttacccta ccactctttt ttactaatcc gttataaaaa tataccttct
      661 gtacaaataa agatattttg ctttaaatat tattccaact cactatatgt tattgttgta
      721 ttattaaact ctttagccca tttaataatt ttttcttttt tatctggctt agtgaaataa
      781 tatccttggg catagtcaca tcccatattt tgtaaacttc ttagttgttc ttctttttct
      841 acaccctctg ctacaacttc tatatcaagg gcatgactta atttaataat ggaaagcaca
      901 atatcctgtt tggtcatttc cttttcaata tcttcaatga aaaacttatc gatctttata
      961 tagtcaatcg gaagtttctt taaatatttt aaggaggaat atcctttgcc aaaatcgtca
     1021 agatagattt gcacgccccg gctgcgcagc ctgtaaagtt tttcaatatt actttccatc
     1081 tcaatcatca aatcagtttc agtaatttca attgcgtatt tgctcggatc aagttcaaac
     1141 tggtcaatat gtctaaataa attatctacg aaattgggat cctgaaaatt ccgggctgag
     1201 atattgatag ctatatttac atctaattct tcttccttat attcatattc ctttatatca
     1261 gaaatacttc gactgataac ccaatctgtt aatggttcaa taagactgct tttttcgact
     1321 ttaggtataa actcacccgg cggtataaaa cctttttcag ggtgattcca tctaattaaa
     1381 gcttcaaagg tagttgcctc cttatttctt agatctatct taggctggta atagagttcg
     1441 aaatggtctt cctctaatga cttattaagg tctcctaata gttcaatatt attatatttt
     1501 atctccacag cttctttctc gtaaatccag aagtttaatt tcttttcttt aactttctcc
     1561 agggctaaaa atcccttttc taacagttca tcaaaaccac taccgtcctc tggataggta
     1621 gatataccca tgataatatc tgtaaaaatc gatatgttct tgaattctac cggagaattg
     1681 agatgaagat taaacttcct taatttttta attaaatctt ttttagattg ttttttaatc
     1741 cacatactat atttgctgtc atttatataa tataattgtt gtcccatacc ttcataatct
     1801 gtaatatgtt caatcataag ttgaatgaaa tccgtaaaat tcctggaacc aactaattta
     1861 tatatatccg ggtaattctt gatactaaaa acaataacat gaaattcttc acctttttcg
     1921 attttttgct ctatatcttt tctaaactta gtcttattgg gtaaattagt actctcatta
     1981 aagtatgcta tcctgtttat tttattaagt tggtgttcca agagatcaaa cagaaaaccg
     2041 gctattgttc caattaatac aaagaaaaac attctaaaag accaattcat agcctcttgc
     2101 atggccatat ttgctgtatt gatcggcata aaaggaccta aaattatacc ggcaatgata
     2161 ccagcaataa aaccacccca acccttataa aaataggctg agagcaggat agggatataa
     2221 attatgtgta gataactatg ttttgtccca ccggtataaa gtacaagtga agcggataaa
     2281 aagaagataa aagcaattaa tattattgtg atcggacgat ttttatagtc tttaattaaa
     2341 gaataccttt ttaatatatg tttcatcgca tattaactcc tctattaatt taattatgat
     2401 ttttatttgt tttgattcgt ttaataatat agctatcctt acattattaa aaactaatat
     2461 tctattaatt tattgatcta aatattctct aaaaataggt tcaggcacca tacttccgcc
     2521 tgttgcccaa atcaggtgag tagaattatt aacggtttta cctctcttac taagttccag
     2581 taattctata tcagaaatgt tattgtaaac ctttgcaagt cctggaaaac cagctaaagc
     2641 agaaggttct aatttgatct tctttttatc atataatctt tttaataatt tagataagat
     2701 atcatcctgg accgtatata taccggtcag taagttttcc atcagtttgc ccacaaattt
     2761 agaaggcttt ccgacggcca gtccgtctgc gaccgttata ttatcgattc caaactcctg
     2821 cacagtaatt ttattatgca gttcagtcat cattccgatt aacatggctg gagaatgagt
     2881 tggttctgca aaaaaacagt ggacatttgg cccgaaaact tttttaaggc caaaacatat
     2941 accacccggt cctcccccta ccccacaggg tagatataca aaaaggggat gattatcatc
     3001 aatctttatc tgttcctttt caaactgctc ttttaatctc aatgcagcaa ctgcataacc
     3061 taaaaagagg tcttttgatt tttcatcatc tataaagtaa gatttggggt ctttttcaga
     3121 ttcctttctg ccttcctcta ctgccttact ataatctgaa tcatactctt ttacaataac
     3181 acccttgctt ctcagcagct gttttttcca ctcccgtgca tcactggaca tatgtacggt
     3241 tgtcttaaaa cctaaagttg ctcctgtaat accaatgctt aaaccgaggt tgcccgtaga
     3301 accaactgcc aatgaatatt ctttaaataa gtctttaaat tttttagaag caaactgttt
     3361 ataattatca tctttatcta tgatattatt ttttaatgct aaggtctctg catattttaa
     3421 tacctcgtat attccacccc tagccttaat agaaccggct atcggtagat tatcatctct
     3481 ttttatatat aattgaccgg gcatatccgt gttaaaaaga tcaactagtt cacttttgaa
     3541 atcatcaata ccttctagag ctgactctat aataccatta ctttctttgg tttcaggaaa
     3601 gacctcttga atataaggcg caaaacgctt gagtctagcc tcagcctctt taatatctga
     3661 cagtgtcatc tctaatacac cattattagg atccttttga tatttaggat ttaaccagaa
     3721 gacttcgtct gtatttatta tctttttcaa taaaggatat tcttcttgcc actgttcaat
     3781 acttttacct tgaatcatct tattacctct tttcccaatt gataaaatta tttaatatta
     3841 ttataatatt aacaggagtg atcacattga atagtcaatt taactgggga aagtttttga
     3901 tatatatcgg tttgataatc gcctttatcg gctttttaat taataccatt gaaattaatt
     3961 ttaactggtt tggtaattta cccggtgata ttagaataga aagggaaaac tttaaattac
     4021 ttatccccat aacctccctg ctaattatta caataatatt aaaccttatt atttatataa
     4081 ttagattatt tatgtagttt aaaaggggta gttttgaaaa ctaccccttt cagtttatat
     4141 atttaagctg aaaaattatc gactaatttt tctgccgcat aatcttccca gtcttcttct
     4201 acttcttttt ctgtataatc atctgtaata tctttgagga actggatcat ctcttcttcc
     4261 tcaatatttt ttcttaattc atttctaaca ttactgttta atataaagtc tgcataccac
     4321 ttataccact ctttatcaaa ccggccgtcc aaaacatttt gttcatactt atgatgagca
     4381 ttttttgcct tcgataaaac gtatctcata tttgatacta aatttttatt catttttcta
     4441 agcctcctct cataagttgt gcctatttcc ttctatatta ataatattct ataagctact
     4501 taataagtcc ttccaggatt tattaaaatt aaagtaggtg ggagctaatt tatctgcctt
     4561 atcaacagct tttccgatgt agttttctgt cttagcccgg ggattttcaa tatgaaggtc
     4621 tttaaatgtt ttacataaca tattagaggc tgccttttct atacttccag ttaaaaattc
     4681 acctggatat ggacttaatt cttctatatc taatttctta aaaccctttt tatctttggc
     4741 tatctctatt ttcttttttt catataaaaa tcggtaaata tcacttttta attttgctac
     4801 ttcagtttgc tttttcccat aacttggtgg taagtgcact acttttaacc gtttatcaaa
     4861 ataaaaatta taaccttcta atccagcgtt aattaaatag tcaatatctt ctccccggct
     4921 gttatagggg tcaaaaggga tcctttctat agtcttgctg ggaattatca tattccctcc
     4981 taaaataaag ggggtcctaa ctagtctttt atctgtgctt tctaattttt tatatgtatc
     5041 attcatgagc ttatttttct tttcaaataa gttatcaggg tttttgtttt cctgcagata
     5101 ttgactccct ttaatgtcag tatagtagcc ggctactccg gcgatataat ctccctctcg
     5161 cctttcacct gcatattgga ccgccttctc taaatacata ttatcttcaa ttatctcgtc
     5221 atcatcaatt cctattacaa tttcagtccc ttgcatatac ggtactaata actgaatatt
     5281 tctgatatta ggataattat ctaaattaat ttcttctaaa aatttatctt ctattttatt
     5341 tttaattttt tcttttaaat caggtaaatc agaataggaa aaatgtttaa tgggaagatc
     5401 atttttatat tgatctacta ttaaatcaac tttagcttcc actttttcct ttaacttatc
     5461 atgggcggct gaacttatta taagcacatc aaaattttta tattctaaat ttttaagact
     5521 aaaaagtaat cttcccaaag tacccttacc atctaaagga gtaggatgat caaagttgta
     5581 tacttccttt ttattatcct gatcagacgg ccaggtccaa tatgtgggaa ttactattgt
     5641 tactttcttc aaattatcac cctttttatt aattactttc tctccaatta taaggaagtt
     5701 cattaatttc gcctggtgta ttacaactat caagcatatc taaagaggct ttaattactt
     5761 ttaactgcag ttttttatta tcaggatcac cgaaaggtgc cccaaatgga aaatttacat
     5821 ttacggttct gggcggtaat actttttcgg ctaattctag gaacatgttt aaagtcacag
     5881 tcgcaatacc attttcctcc agttctcttg ctatcaatcc cacggattga ttacacaccg
     5941 gtcaggtggg ggaaattaaa gcggcatcta tatctttatt tttcagtttc ttagctaatt
     6001 ctacagcatt ggctttaagt tcttttattt ttaaacagta gcccataaag gaataatttt
     6061 cgtcagctac agctccaatt tcctttttat tttctaattc ttttaatagt tgtagtggaa
     6121 aggcacagtt caagtcctta ttcacatatt tgtggtcata atgggtatga gaaatcttta
     6181 ttaattcagg ttctgtatct tttggtatca tcctataaga aggatcaccc ataggatcat
     6241 cagtatcaaa aggttcgtca ttttttaaat gaaaccctcc tgaggatatc aatactatat
     6301 ttgccgtgtt aatcttctta tccaatttgt taaaaggcac ttccttatcc ctgttatctt
     6361 caaaatcaaa atcaggatca acttcctctc gcatgaactc tacaatcttt ttaagatcca
     6421 taatatctcc tttctattga ttaaatttaa cccctaattt gatactttca gcgggatttt
     6481 tatcgacata atatctataa ctctccaaga tatctttaaa atcaacaaca ggtgtaatga
     6541 tatctctaca gtcataatgt cctcgtgata acatctgcca gcaggtcttg tttatcctct
     6601 ctaaatccca gcggggatgt tctctattcg gttcactaca ggccctacta aatattattt
     6661 cgggtatatt gaaatgggct tctcgtccta aattcaactg tcctttgagc tctttatacc
     6721 aacctacggt ggttacatta cctccgtaag cggtagatct gatagccgtc tgtaagacgg
     6781 gtgtatgacc actggtatct atggagtggt ctacaccctt cttgttcgta ttttgcttaa
     6841 tttcatatcc tacatctgtc tgattggggt ctaagacaag gtctgcccct aatctatctg
     6901 cagccttcct tctcttttca attgggtcta cagctgctac ccagaccgcc cctgccttaa
     6961 gagccatttg aacagtcagt aagcctattg cacctagacc aaatactgcc accctatcac
     7021 cgagtttcaa actgctgtcc ctgattcccc ccagggcaaa acgggccggg tcataacata
     7081 aagcactctt ccagttcata ttctctggca gtaccaaaac atcttcttta tttatatact
     7141 gatattcttt taatcctcca taaccggcta ccctttgtcc tatctttaaa ttcttaactg
     7201 cttttccttt ttcaactatt aaacctaccc acatattgcc taaatccatg ggaaaaactt
     7261 cctctttttt atcttttttt aaaaacatat cccaatcatt atcataatat tctttttgga
     7321 aagggctgac acctttcagc atagtcattt cagtcccgtg tttaggtgag ccgtacttca
     7381 cccttacctt aacctcatta gctttaagat cactttcttt aaaatctttt aatttaagag
     7441 aatctacttt atcagcaatt aaacctctgt acataatatt tccccctaaa tattttttaa
     7501 aatatatttt aaactttctt ttaactgttt tgtgggattg tccggcttta gggcctcaaa
     7561 agaaagatat ttattatatc caatagattt taaggttgca aggtgtctag gaaaatcgat
     7621 gtggccctgc cctggtagtc tccggttgct gtccgagaga tgaatatggt ataagtagtc
     7681 tttagcctct tttaaactct tatcaatatc tttttcttcc atattcatgt gaaaagtatc
     7741 tgccattagt ttaactccat tattatctaa cttatcaata atttcaatac cttccgataa
     7801 cttattgata taatgagttt ggtatctgat taagggttct aataacaaat aaaccccgct
     7861 ttgtaccgcc tgtgctccca attcatttaa tgtatcaata agtaactttt cgtcttcttt
     7921 aagggatctc tttctgggag gatagggtaa ataatcagaa gtaccgtaga tagtgggagt
     7981 taatacacct atcgccttgt attttgcagc ataattcaaa agctctttta tatcccggac
     8041 tgcttctctt tgtttttgct tatcttcaga taaaagccaa ccccgatggc cggcacaaat
     8101 tattgctaac tcaatatctg tactatcgaa agcttcatta acttcggtgg gattctgtaa
     8161 aaacttttct cccgagattt caataccgtc aaaaccgagt tctttcgtgt taagtaatat
     8221 cttttttata ttatctccgg gtattaaagt atcctgacag gctatcttca tattaaaaac
     8281 cctccttttt aacaccttgt ctttattata catcatatca agaaaattat taatgggagg
     8341 ctgaaattta cgaaaagcaa ttactttatc ctgttacttt ctgttcatag atatattagt
     8401 taatattttt gttgtgtttt ttgatacttc tataacaata attatctgat taatattaca
     8461 atttccccac ctattaaata ttttttcact ttgggctgct ttttaccaaa gactgcctgt
     8521 ctgccttttt caacatctca attgtattaa attttaaaat tattttaaca tctgattttt
     8581 ttacgataaa tttaatttga aaaaccccct tataaattta taagggggtt tgttatttat
     8641 atgcctattt ggtctgatct aactgctcaa acatagctct atttagattt ttgaaagtac
     8701 tttgtccact tcccaattca ctgatctgtt tatccatcat atcagtaaat acttcttccg
     8761 catatcctcc atcgatatac ttgttatcgg gtaaagttga acgcattgat ttaaacatta
     8821 actttaaaaa tacagccgaa aaatctgtaa tggcttgctt taatttttcc tcatcttcat
     8881 cggcttcacc atcattatta aagtttctta tattttcaat ctgctgatta tcttcagatt
     8941 ggttttttat taagtttgac ctcattgact ggtaaccctc tatattcatc tattcatcgc
     9001 tccccgttag attaattcca acttagcatg taaagcacca gcggccttaa tcttctggat
     9061 tatcgaaact atatctctag gagtagcacc tattgtatta agggcagaaa ctagatcttc
     9121 gatagtattt tcattgctga tcattactat attgccctct tcttctgaaa cttcaatttc
     9181 agtttcttct gtctttttgg tctccccttc tgaaaaagaa ggtggttgag atactttttc
     9241 ttttgtctta atagttactg aaatattacc gtgagctacc gccactgtgc ttatcctaac
     9301 attatggctt aacacaatcg tgccggtttt ttcatcgata actattttag cttctatatt
     9361 agatcttaca tcaatattgt ttacctgggc aataaaatcg actaccctac cttcaaattc
     9421 atccggtaca tctactctaa ccgtaccggc atcactggct ctggctatac tatcatattc
     9481 cagtttttta tttaaagcct ttgcaatatt atctgctgtt ttgaagttgg gattttgcag
     9541 taagagattg atatgatctt tattcaaatc aaactctatt tctctttcta ctaaggctcc
     9601 gttgggaatt cgcccgacag tggggtggtt tttccttgca ctactaccac cgctctgtac
     9661 attaaatcca ccaattgaga ccggcccttg tgctactgca tagatctgac cattagcagc
     9721 ttttaaaggc gacattacaa gggtgccccc ctgcaaactg tctgcatcac ccatagaaga
     9781 aactgttaca tctatccggt caccggaatg aacaaacggc tttagatcag ccgttaacat
     9841 gacagcggcc agattcttac ttctaatttg gtctggggtt acattaatag aaaactcccc
     9901 catcatattg gccacactct gcacagtggc ctgactccgg ttcgaatcac cggttccggc
     9961 caggcccaca acaataccat atccgatcag ctggttgtct cttatcccct ttattctagt
    10021 tatattaccg atcttaacaa tcgggtcctg gttttgagcc tgtatgttta aggttaaaac
    10081 cataggtaat atcaataaca ctgtcaaaat cagtagtgat ttcttgtatt tcaatttcaa
    10141 aataataagc tcctttctct taaaagatcc agtttacaag cttttctaaa aaaccagttt
    10201 tttgcttatc tccaaccggc ccttctcctt catactctat atctgcatta gcaatcattt
    10261 tggaagagat tgtattttct aaagagatat cagccggacg gattactccg gtcaatttaa
    10321 tagactgttt ctcaccgttg atatttatgt tcttggtacc cgatattttt agatttccat
    10381 tttcttttat ttcaacaatc tgagtggtaa tatctgcttc taaagtaccg ctgcgggaag
    10441 tttgcccatc tgctgagccg gaatcagaat acccaaagcc gaaggccttt aagaaactaa
    10501 agattccaaa acccgcttct gcatcaactt cacttgcctg gctggtactt gtattagcac
    10561 tctgaatagc attggcgtct tccttaatta aaacagttat tatatcccct ttaacatgtt
    10621 ccttgcggtc tttataaata tctccggact cttcactcca gagtgaatta ccttctgcca
    10681 aaagagcagt tgtaagtgta aacattataa tgggaatcat cattattgtt agtagctttt
    10741 tcaattttat tcccctttct aatccttaat cagttctacg agttgggggc ttattacttt
    10801 tgcttgaaac ttatacctat ttttggggtt ttctacattt atatattgac cctgtttacc
    10861 tctctcccgg gccaccacca ttaaggtaag ttctacatcc ccaacaatta ttttagcctg
    10921 aaccctatct ccccagttta ttaaataggg agtattatag ttattctttt ttaaaatatc
    10981 accctgttta agtgaatagg aaagttcaat attctgagtg aatatatttg accctttttt
    11041 aacaaacttt tcatcttcaa tactatcaac ttcaaccatt ttatatacga aatcatctct
    11101 ttttaactta gtcccctgag aaatatactt tttagctaca tatgcttctt tatatacttt
    11161 aatattaacg gggatataaa atcgtttgta ttcttttcct gcaatattaa tggccaccgg
    11221 tattgtcatc tggccgggct ctatatccct attttctaag atttctagtg aatatgcctg
    11281 cttcggaatt gtaatttttt cccgccgcag atttacttct atcgtaaatt tatcagaatt
    11341 atctcttaaa atactgttta acttcttttt taaaaattcc tttattttct cagcttcaat
    11401 ctgttttgcc tctcttctta ctattatctg ctcgggaaaa ttaagtctaa attcatctgt
    11461 tttaaatcct tgttttttta acatcaattt aactaaagct ttatttatat acttcttata
    11521 accgggcaag ggcgctttac caaggtctat ttcattgatc atcttttctt ctgcagctgt
    11581 tatttcatta aactcagcaa tttttccgag ttttatgctt tcttccttta tcctaacctc
    11641 tttttcaata attacttcaa aggctgataa aggaactgtt attgaaatta ttaacattat
    11701 tgttaaggct aaaatagacc ttttattttt caacttgatc agtctcctat attatctcac
    11761 tatcttctta attgattggc ggtctgcagc atctcatcag ctgccttgat tgcctttgaa
    11821 tttacatcat aggctctctg agccgagatc atattgacca tctcttccac tacttttaca
    11881 ttggacattt ctaaaaaccc ctgactgata gctccaaaac cctgttctcc tggaaccccg
    11941 gtctgtggat ctccggaagc aacggtctgc tgaaataggt ttctacctag actatttaaa
    12001 ccggctggat tggcaaacct atatagctgg atttgaccaa tctgttcggt tgtattaccc
    12061 gtttttatat taactgttcc atctgatgtt atggaaatac tggttgcatc ttccggtatt
    12121 actatctcgg gctgtaaggg ataaccatct gctgttacca ccctgcccgt actatctctt
    12181 ttaaaagaac catttttcgt atatgcaacg gtaccatccg gtttcattac ctggaaaaaa
    12241 ccgtcacctt ctactaataa atcaaggggg ttatcggtat ttcttatatt cccctctgta
    12301 aatatttttt gagtagaagc aacttttgaa ccatgaccca cttcaattcc aaccggaatt
    12361 tgcgccccct gtgcattggg agtaccgggc tgtctaatct gtctgtacat taaatccgca
    12421 aaattaactc tgcttttttt gaagccacct gtatttacgt ttgccaggtt attggaagtt
    12481 acatcaatac ttgtctgttg agtgttcatt cccgtcgcgg ctgtccataa agcacctatc
    12541 atttgaaatt cctcctatga ttttttgtta actacctctg tcagtcagta gttgttatgc
    12601 agcttccaat cactggtcca gctgcacctt gttatttatc ctaatcttcc cacatcatta
    12661 acggctttat ccaaactatt atcgatagtt tgaataacct tctggttagt ctcataagct
    12721 cttgtagtct gaatcatacc tgccatttcc tgtacaatat taacatttga actttctaaa
    12781 taaccctgca taacctgaac ttcatcagca ggcatgccct ctataccatc agctgtttgg
    12841 tatagattgt caccaacttt tgataagttc ctgtaatcag caaagtcaac gatattaatc
    12901 ctctcgcctt ctaattcacc taagtaaatc tgtccggact tgtctattga tatctcccgg
    12961 cccggaatgg tctgaatggg ctcactgttt tcatttaaga gtagatgacc ctgttcattg
    13021 attatctgat tttgagaatt aagcgtaaag tttccattgc gtgaatatct tattccctgg
    13081 ggagtctgga ctgtaaaaaa cccatctcct tttaatgcta tatctaactt attattagta
    13141 tgttctattt tgccgtgtga atagtcggta taattttctg caatatcaac acctgaacct
    13201 aaagcaccaa tctcggtttg gccgttactg tcaattcttg aaatcaacat ctctgaaaat
    13261 gttctttgta cactattaga cttcttaaaa ccatctgtat cagcattggc caaattgtta
    13321 gaagtaatat ccattctcct catattggtc tgcatggctg aagcggccgt atataatcct
    13381 tttatcacaa catctcacct cctctgtaag aatatagttt tgcaatcaat cttgtttctc
    13441 taatacccat ttttaaattt tttgcgatct tatcaatact tttcttttga ttgaattcgt
    13501 ctaaagcttt cttgtgtttt ttgtttaatt tttcgatatt atccttatgc ttttctgtaa
    13561 actctctttt tgcaataagc tgtttttctt ttctcttgtt cagggaagca ataactttat
    13621 cattgtaatt tagccgagta ttgttataaa aaacattaat cgccattaaa attactaacc
    13681 aggccataaa atcacaccaa tcttaaacct taacatcgat aacagacccc ttaatttttt
    13741 tatttttatt ttcttttgac tcatcttgat cttcttcttt ctgctcttgt tttcgcttat
    13801 tttgattggc tccatcctta tcaatagtca gattttcact tttttctttt ttattaaccg
    13861 ttttttgttt ctttttatct ttttctttct gcgcctgatt taaagtctgc tgttcattgg
    13921 ctgaatgtac ctgtttcatc tgttcaattt tttctacctc aagggacatc ggtattaatg
    13981 tatttggatt aatcggatcc gacatttaac cccctccttt aaagaccaaa ccttctcaat
    14041 tctccttctt caactttaaa tacggtacgg gtatgaactt catctacctc atggtggtat
    14101 ttgcctattt ggacctttac acccggatat actttccgtt tgaccataac ctcaccctgg
    14161 gtagccttat taattttttc agacagttca tttacggttt tctcattttt gtttatcttc
    14221 tcttttaaat cacttttggt ctctatcagc ctatcataca tattggcttt ttcttccggc
    14281 agttgaccgt acttttcttt catctgattt aatatattga tggcttttac cgtttttaga
    14341 tagttggact tacattcggt taagttttct tttgccttat tacagcggct tctggtatga
    14401 gggtctattc cggctgaaac aattgtttta gtagccagac tggatccaat aatattagct
    14461 ttgacctctt tagttgccct tacttctccc cctacgatta gacctttatg tccggttaat
    14521 tctatactgc cggctgcatg gattttgctg tgcatgattg catccttaac aaatacatct
    14581 cctttacagt tcacggtccc attttggact gttttggtgg taaggtttcc ttcacactct
    14641 atttcacctt tatttttagc aatgaaattt ttatttatcg taatatctcc actgcatttc
    14701 agatgggcgc cttctacatt gccccgcaca tggatatcac cttcggcctt aacttcaaat
    14761 ccttcggtca cattaccttc aataaacaca ctaccgttaa agtcaatatt accggtattg
    14821 aagtccacat cgccctgaac tttatagacg gtaaggatgt taactttatc atttttataa
    14881 gaaatatggc ctacttcggt tgatactaag gtattatctt cagtaatttc agtattttca
    14941 cccttaggta atttaacatc ttctggtttc tcaggtgtaa tggttttacc ctcaatattt
    15001 ttaccttcct caccttcggt agcttcaatc ttactaacca gtttttcgcc ggcttctacg
    15061 ttattaacaa taccgagatc gtgaaagtct atgctgccgt cttcctggac agttcccaca
    15121 tttttcccgg aacggtcaaa gtgaaattct aacctggcat cctcacctgg ttgagcagct
    15181 ttcccttctg caataactat atcttcaaga ggggcttcgc tttctaagat aagattaata
    15241 ccgtaatctt ttaatccata aacaatatca ttagctttta actttgcttt gatatcttct
    15301 tcagtaaaat cttcacctaa tccctgggga ggaatatagt ctacaaaggc tttcatctta
    15361 tcggcggatt ttttaattat aagttctgct tcttcatttt tttgataagt actcaaatta
    15421 gtcttgacaa cttttccgat atccagggct aactttttat caatctcaag tgcatttctt
    15481 aaaagattga aattaatatc ttttttgggg tgtaaagtta tctggtatct ctttttactt
    15541 cgcaaacctg aaaacatgcc atctttttct tctactttta gatctcttaa tttaattttt
    15601 ctttctttgg aaagtttttg ataagctttt tgagctgctt tctctttact ggcagctttt
    15661 actactaaag aatccaatta atagtcaccc ttcctagtta attgattatt ggaactcctc
    15721 tttttttctt gatagataac ctcgtaatct atatactgct tttttgtgta tttgtgatat
    15781 tctagcaggt gataaatcca taatctctgc tacttctttt tgggttagct cttcataata
    15841 tattaaagaa actactaatt tttgtttatc gctgagattt acaattgcat ttttcaatat
    15901 tttaatggca tttttcttat caagaatctt ttggggctta ttaaccttgc tgctcggcag
    15961 ccagtcaatt aaagatttac cgttaagttc tgaataaatt gatacccact gcgattcctc
    16021 taactttttt aacaccttgg taatctgatc ttttgaaaag tccagttcag ctgcaatttc
    16081 attaatctta ggacttctat tcatcttact ttctaattct cttgtcttgc ttttaataac
    16141 tttagctttc tgccttaaag aatgaggaag ccagtctaat ttacggagtt gatcgattat
    16201 ctctcctttg atcctttttt gggcataact attgaattta tagccccggc tgggatcaaa
    16261 cttttcgata gcatcgatca gaccaattat tccataactt tctaagtcct ctagttcaat
    16321 acggtccggc acataagtat taaccctacc tgcctgatac ttaactagat ctaaatattc
    16381 cattattaac tgttcacgtg ctttttgatc gttctcttct ttaaagcgcc gccatagttc
    16441 ctggtttgac agcatattat acctacctca ttctattatt aattattttt ttcatactca
    16501 atcacaggtg gttccatttc actgaaatca tcttcaaagt cccagttatt ttcctcatta
    16561 tctttatctt ctttttgttg ctctccaccc gttgattcct cttgagcatt agcctggttt
    16621 tgacttgcct ttgttttttc atattcacgg ctcgatttac cctgcggctg cttttgctta
    16681 ttatcatcac tactatcttg gttagtggct tgcgtttttt tattcataat ttccattgca
    16741 taggtaatta ggaaggtaaa aacaaaagcc gttaaagcga acttgactcc tgaaatcaaa
    16801 acagaagaaa gtggattaaa ctgtatcaat ccaataagtg ctgttaacat gtatactatt
    16861 aacgctgcaa atgttgaacc tgtcaattta agtttcatct tagatcatcc cacgccttct
    16921 caattttctc tggtaattaa aaagaaatga aatgatttta tctctatctt ttctttccat
    16981 ctcaataaat tctatgccgg ctgttttgct gtaaccgtct tgattatgtt tgattcttac
    17041 tatccggccc ttgactaatt tatcaaggtt ttctatatta agattaagta ccaatttttc
    17101 ttttaaattc aagtcatctc ttaaaaccat gagtaaaccg ccgccgctga gatcaacagc
    17161 ggtagttttt ttgaattccg cttctttttt ttcatcttca atatctttta aacttttata
    17221 ttttatcttt ttattaacct gtaatctgaa atatctccga cgttgaatcc tattaacttc
    17281 attagttctc tttaaaatta aggtcgggat cggatatttt tccctaccca aaatttcagt
    17341 tgttaatgaa taaaaggcgc tatcaccttc atataggagc tgaacctcta cccctttcct
    17401 taaaggtata agttcctctc ttttatagat agccatcacc tttatttttt cctctaagag
    17461 gtcagacact ttgctgttat agctgccctg ataaggcccc tctgagatta ctacttctat
    17521 attttggttg atacttaaat tcttgtcggg gttagacatt aaacacttta cctccctggg
    17581 gcaatctgtc cgattaattt atagaagaaa tttttaatac ctcttgatgg tagatctagt
    17641 tcttttccta tcaagttccc ggcgagctgc aagaagtaat catttacttc tgactcggga
    17701 tagagtttaa agactgggtg ctgtttcttg attgattttg agattaagtt atcatttggt
    17761 attgtcccca gtaaatctat atttaaatct aaatacttgc gggcagttgt aataaatctg
    17821 tcgattatac tgtctgcctc tttttgagaa tccactctat ttacgaccag gtgtaatttc
    17881 ccccggtact ggctgctgct taatagcttt attaaactat aagagtccat gattgaagtc
    17941 ggttccgggg ttaaaacgat caatatctcg tcagctgcag tcgtaaagtt gattacagac
    18001 tggctggcac ctgccccagt atccaatagt attacatcat aatttctttc gatctctgta
    18061 acaccactta agaggtgttt tatattttca ttactgatat taagaacatc atctataccg
    18121 gaaatacctg aaaggacatt taggccataa gggccggttt caacggcctg ttttaaagaa
    18181 caaagacctt ttagtacatg gtttaaatca tactttgttt ttaaacctaa caggacatct
    18241 atattggcca tcccgatatc gctgtcgatt aaaaaaacct ctttttcaag ttctcttaaa
    18301 gctaaagcaa ggttaacagt aatagttgat ttgccaactc cccctttgcc gctagccacc
    18361 gctatgatcc tgctctgttt atccttatct ctgtttccta tttcatcaaa gtccttaata
    18421 ttattacttt ccatgtaatc tttaagttta gcggcctgat tattcattaa aatcacccat
    18481 taggtagtta tagaggattt cagagttagc cttatcaata tcttccggta catcctgccc
    18541 aaaagttata taggaaaacg gcatatcata cttttggctt atattaatta catctccata
    18601 agttgatgtt tcatctaatt tagttaagat cattttatcc ggcttgagtc ggctgaagtt
    18661 atcaaccgca aattctagat cccggctgga catgttgaga cttaccatca ggtgaatttc
    18721 atcaactaag ctatctttag taaattcatt taactggcct aattcccatt tgtttttcca
    18781 gttgcggccg ggtgtgtcta ctaaaatcaa atcggcttct ttacggcatt ctttaaccgc
    18841 ttttttaaat tcatctccat catagactac ttttaaagga atattgataa tatcactgta
    18901 agtacgcagc tgctgtacgg ccgcgatacg gtatgtatca gccgtaatta aatgtacttt
    18961 tttgccttct ttgatggcaa actcggccgc aatcttggct attgtagtag tctttcccac
    19021 ccctgttggg ccgacaaagg taattacctg tgtttcttca gctgctttta tgggaaccgg
    19081 ttctcccagt aaatcttcta aatggttttt tgactttaat tttagattat tctcatctaa
    19141 attactatcc tcacttaagt aatctgtaat tgcctttaca ttttcttctc ttacattctg
    19201 cagccttagg tactgctgga aggtcgttaa aacctcaggg gtttcctctt ttaccttctt
    19261 ttcatttaca ttattactta agtcctggat catttcttta agatggttga tttctttttg
    19321 ggtgttttct tcagcttttc tgggttcttc aaggccagca agaacttcta ccttttcctg
    19381 accgaaaaaa cctaaaaaac cgcctttggt aaatttacgg gtatcgagaa ttatcgcatc
    19441 tgatcctaaa tctactttta ccttaaatat ggtatcctga atattatctc ctatatattt
    19501 ttttaccttc atttaattca ccaccgttcc taatacttct aagttgacat ttggcgtcag
    19561 ttcactgaaa gataaaatag tcagttgagg aaaggaccga cttgttaatt ctttgagcgg
    19621 ccgcctgatc atcggggaag taaccagtat tggttcttta cccttctgta ataatttctg
    19681 cgcttcattt gaaatcgatt cgattaactg ctgggcctgc tggggctcca ctgccaggta
    19741 gttaccctgg tcggactgtt caacagcctg tcctagattt tcttccaact gaggatctaa
    19801 agttatgacc accagctggt tattctgatc gacaaactgg tttgtaatct gtctggataa
    19861 cccctgtctt acatattctg ttaagaggtc tatatttttt gtagtcctag cataatcagc
    19921 caaggtttct aatatagtga ccagattgtt gaccggaata ttctcccaga gtaaattctg
    19981 caggaccttc tggatctctc ctaagcttaa taggtcaggc agcagttcat taacaaccgc
    20041 tgaattattt tcttctacat tatctactaa atctttaact tcctgtcttc caattaattc
    20101 atgggcatgt tctttaatta actcagttaa atgggtagcc attactgagg gtggatctac
    20161 tatcgtgtaa ttggccatct cggcctc
//
LOCUS       DATGCJ010000141         9615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_285480, whole genome shotgun sequence.
ACCESSION   DATGCJ010000141 DATGCJ010000000
VERSION     DATGCJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285480"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1342)
                     /locus_tag="VKN64_07165"
     CDS             complement(89..1342)
                     /locus_tag="VKN64_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60056.1"
                     /translation="MDIKKDVNIWKFSFYGFFKNLKFFEPFLYVYFLSIGLTYFQIGT
                     LISIREIIKNVLEVPSGIFADHYGRKSSMKLCFILYIISFFIFYLGYNFIILIPAMLF
                     FGAGEAFRSGTHKAIIFSYLDYKNISDQKVEVYGRTKSYSLLGSSLSAILGAVVVFIE
                     SGYRYIFLLSVLPYIIDFFLISSYPDYLEGASDKSLKFSEIKEGIKSSFKNIKDIVHL
                     RRGLINSGIYDGYFKVLKDYLQPLLKTQLSVLTIGYMFATEEKMLSVLLGISYFVINI
                     ITSFTTRNSFRLKKYFSGSFKVLNIIFILTAILIGSIGFLYWRNLPILIIVFYLLYYI
                     IRNLRRPFMLDYLSSVIEKEERATMLSIESQLRTVIIIILAPLLGLVADKWGLWIMFE
                     IAFVTMAIIYLLISLSDINLITKED"
     gene            complement(1382..2356)
                     /locus_tag="VKN64_07170"
     CDS             complement(1382..2356)
                     /locus_tag="VKN64_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HMA60057.1"
                     /translation="MKKVVETLNHNWESKLANNIVNLWDHDKNTVKYFRSSANFIFTF
                     QKRNNRFFLRFNKSSSKSKRELQEEIKLLKALNERKENIVKPVKSVRNNYVETVKTKL
                     GEYNAVVFEEVKGNRFEFEELGKKNFYIWGKELGNLHNTTNKIESNLSLNFNSWEDHL
                     IFIENNLPDEETSALEELKKIKDRMYNLKIDNKNYGIIHFDFELDNLIYNNKRFYIID
                     FDDAAYYCYVADIAFALRDLFAGKVDFNNDMLKAFIKGYKGEFIIEQDKLLDLFLFLR
                     FHNLLTFTKLIKAVDINEDSNQPAWLINLKYKLNDVIRGYRNSFEKAN"
     gene            complement(2506..2892)
                     /locus_tag="VKN64_07175"
     CDS             complement(2506..2892)
                     /locus_tag="VKN64_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMA60058.1"
                     /translation="MSLKVAIATDEGERFVNRHFGDADYYYIYEVDEDKAEFILKIAN
                     NSIEEEKHADPKKAKSIVKILKEEDVQVGVNLQFGPNIKRVKKHFVPVVIDTESIKEG
                     LDKLQKNYHELKKLWEQGEKRSYFKF"
     gene            complement(3066..4568)
                     /locus_tag="VKN64_07180"
     CDS             complement(3066..4568)
                     /locus_tag="VKN64_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA60059.1"
                     /translation="MFKKIIVLFTLALLLFSFGAGAQEKVLNFATSSEIVGLSPVLTN
                     DNVTSDVLAQIYDTLFVRNPETNELEPSLAVSYENPDSKTWIIHLREGVTFHDGTPFN
                     AEAVKFTFERIGDPEVASPRASLVEPIESIEVRDEHTVVMKTKYPYGPFLATLTHSNA
                     SIVSPTSVEKYGDLMQNEAGTGPYVVDEWVSGDHITLKKNEDYWGETGNVDKLVFKIV
                     PEAATRIAMLETGEVDFLDTIPPEHLDRLKNNSDIEVKLVQGSPIRYFGFNFKKEIFN
                     NLKLRQAIAHAVNQEALVSTLNGLGYASHGIIGPKVFGYKEEIEEYGFEYDLEKAREL
                     LAEAGYPDGFETTIWSTSYDTYYQRMAQILQAELKKIGIDANVKLLEWGSYLSATQKG
                     EQDLFLLGWSNLTGDGSELIYPNLHSDNLGGANRSFYEGADEMITATRTTVDQEERLR
                     LLHEANKYLVEQAVWIPLYHQTVVAATRNNVSNLIIKPNGEWLVKDVMLD"
     gene            complement(4679..5554)
                     /locus_tag="VKN64_07185"
     CDS             complement(4679..5554)
                     /locus_tag="VKN64_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60060.1"
                     /translation="MKDKKEKREKKEISIQSKRWSKFKRNKMALLGSFLILLQVFIAI
                     FAPFLAPYDPYEQNLMKMTQPVGTEGHILGTDNFGRDILSRLIFGSRISLVVGLTSVL
                     FGLFIGVTLGLIAGYYKKLDNIIMRLIDIMLSFPGVLLALAIIAMLGPGLINVIIAIS
                     IWSIPTFTRLVRSQVLQIKEYEYVMAAEALGVKDPIILIKHILPNCIGPIIVYATLRI
                     ASAILSAAALSFLGLGAQPPLPEWGAMVSAGRSFIYNAPHMIIVPGLAIMMLVLSFNL
                     VGDGLRDAFDPSNLR"
     gene            complement(5570..6511)
                     /locus_tag="VKN64_07190"
     CDS             complement(5570..6511)
                     /locus_tag="VKN64_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60061.1"
                     /translation="MISYIARRLILIVPVLIGVSILAFLMTVLAPGDPAAIMAGPQAS
                     QSDIENLRTKLNLDKPIHIRYFTFLKGILTFDLGQSVQSQKPVSEMLLTRYPNTLVLA
                     TASMIIATLVGLITGIISGVKQYSIFDYTSMFVALIGISFPTFWIGLVLMYIFSVKLG
                     ILPVSGLPAEWWTIDGLRHLILPAITLGVYSAALIARMTRSSILDILQNDYIRTAKAK
                     GVKGKRIVFKHILKNAFIPVITVIGLNFGRLLAGTVITESVFGINGIGRLAVRAINTR
                     DFPVVQGCVLTIAFTFVFINLIVDILYAFLDPRISYS"
     gene            6949..7740
                     /gene="nadE"
                     /locus_tag="VKN64_07195"
     CDS             6949..7740
                     /gene="nadE"
                     /locus_tag="VKN64_07195"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HMA60062.1"
                     /translation="MIDENLGEEIKEWMKEQVDKRNAEGVVVGLSGGIDSSLVAALCK
                     EAFGDDLLGVLLPASGTISKDIEYANMLAEEFDIETMTVNLEEINDKIEETFHNIDIT
                     KAEREYCPQSKIASSDPAEQNIQTRLRMLTLYYIAEKLNYVVMGTSNKSEILSGYYTL
                     HGDEATDMRPLGDLTKTQVWELSEQVGVPEEIINRDPSGGLIGSETDRDEKDLGVDYS
                     TFDKIYEALENDGDLSQFDEDDVARTKELIEAAADKEKTPTFKLD"
     gene            complement(7859..8509)
                     /locus_tag="VKN64_07200"
     CDS             complement(7859..8509)
                     /locus_tag="VKN64_07200"
                     /inference="COORDINATES: protein motif:HMM:NF020475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HMA60063.1"
                     /translation="MFDKNKMHSICAKCDKKTCSIDSNDYPKWCLQGNGNWLTDALKK
                     YEKEENMKLYKTAAHIETTGYKKWPRLKEIAEYAIAADFKNIGIAFCIGLEKEAKIAT
                     KYFEGRGLEVNSAVCSCGSIDKADLDIPPEDRFSPEGFEAGCNPIGQANLLAECETDF
                     NVVLGLCVGHDSLFFKHSKAPTTVLAVKDRVLGHNPLVAVYQADSYYSHLYESEEN"
     gene            complement(8837..>9615)
                     /locus_tag="VKN64_07205"
     CDS             complement(8837..>9615)
                     /locus_tag="VKN64_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60064.1"
                     /translation="GEELVGTYDEDGYVEGINEVTVTTELLREYSTSEDIVWGYPDNV
                     INETVNIKDISELFGKVNLGTVTAPSNHTFMYDGCFKWEDYGGSLCGDYQYDNTATII
                     ETQQSSSASLKVNVQCYIDETAYAMGESAKCFLNYQFSQWGWTNPIVPSENEYVWPLY
                     TGAAFCDPDKGENVGTVKFEYEDGDIKNVKFNLDWPYEIEEYHIYAGKDMFPQQTRGK
                     KTIDTVAPGQYYIEDDLSGEIYVIVHAVVKMPDPNFGPNQ"
BASE COUNT         3213 a         1803 c         1386 g         3213 t
ORIGIN      
        1 aattgttcgc atatcctaca ctcctctgtt atttttttat ctatctttaa aaaagataat
       61 gttaataatc ttctatataa aattattatt aatcttcttt agttattaaa ttgatatcgc
      121 ttaaactgat taataaataa attattgcca tcgtcacaaa tgctatctca aacatgatcc
      181 ataatcccca cttatcggca actaagccca ataagggagc taagatgatt ataatcacag
      241 ttcttaactg ggattcaatc gaaagcatgg tcgccctttc ttctttttct atcaccgaac
      301 ttaaataatc cagcataaag ggccttctta aatttctgat tatgtaataa agtaagtaaa
      361 atacgataat taaaatcggt aaattgcgcc agtataaaaa ccctatacta ccaattaaga
      421 tagcagttaa tataaagata atatttaata ctttaaaaga accactaaaa tactttttca
      481 acctaaaaga attcctggtc gtaaaagaag tgataatatt tatgacaaaa tacgaaatac
      541 cgagtaaaac ggaaagcatc ttctcttcag tagcaaacat gtaaccgata gttaaaaccg
      601 atagctgagt ctttaaaagc ggctgtagat agtctttaag cactttaaaa tagccgtcat
      661 agatacccga gttgattaaa cctctgcgaa gatgtactat atcttttata ttcttaaaac
      721 tacttttaat accttcctta atctcagaaa attttaaact cttatcagaa gccccttcta
      781 aataatcggg gtaagaactg attaagaaaa agtcgattat atagggcaaa acggataata
      841 gaaagatata ccgataacct gattcaataa atacgacaac tgcccctaaa attgccgaaa
      901 gtgaggaacc cagtaaagaa tatgactttg tcctaccgta aacttctacc ttttgatcgc
      961 taatattctt ataatccaaa tagctaaaaa ttatagcctt atgggtgccg ctgcggaagg
     1021 cttccccggc tccaaaaaag agcatggccg gtatcaaaat tatgaaattg taacctaaat
     1081 aaaagataaa aaaggatatt atgtaaagaa taaagcatag tttcatcgaa cttttacgac
     1141 cgtaatggtc agcaaagata ccagaaggta cttccagcac atttttgatt atttccctga
     1201 tagaaatcaa agtaccaatc tgaaaatatg ttaaaccgat agataaaaaa taaacatata
     1261 aaaagggttc aaaaaattta agatttttaa aaaaaccgta aaaactaaac ttccagatgt
     1321 ttacatcttt ttttatatcc aagaaaaacc acctcagtta taaatcctac cagatatatt
     1381 tctaattagc cttctcaaaa gaatttctat aacctcttat tacatcattt aatttatatt
     1441 tgagattaat aagccaagct ggctgattac tatcttcatt aatatctact gctttaatta
     1501 atttcgtaaa ggttaaaaga ttatgaaatc ttaaaaagag aaataaatct aataatttgt
     1561 cctgttcaat aataaattca cccttataac ccttaataaa tgcttttaac atatcattgt
     1621 taaaatcaac tttccctgca aataaatctc ttaaagcaaa ggcgatatca gccacataac
     1681 aataataagc agcatcatca aaatctatta tataaaacct cttattatta tatataagat
     1741 tatccagttc aaaatcaaaa tgtataattc cataattttt gttatctatt tttagattat
     1801 acattcgatc tttaattttt ttcagttctt ctaaagctga agtttcctca tctggtaaat
     1861 tgttttcgat aaaaattaga tgatcttccc aactattaaa attaagagat aaattacttt
     1921 ctattttatt tgtagtatta tgaagattac ctaattcctt accccatata taaaaattct
     1981 ttttccctaa ttcctcaaat tcaaaccgat tccctttaac ttcttcaaaa actacagcat
     2041 tatattcccc taacttagtt ttaacagttt caacataatt attacgtact gatttaaccg
     2101 gcttcacaat attttctttt ctttcattta aagctttaag taatttaatc tcttcttgga
     2161 gttccctttt tgatttcgat gaacttttat taaaccttaa aaaaaatcta ttatttcttt
     2221 tttgaaaagt aaatataaaa ttagcacttg acctaaaata ttttacagta ttcttatcat
     2281 ggtcccataa attaactata ttattggcca gtttactttc ccaattatga ttgagtgttt
     2341 ctactacttt tttcatagta ctcaatttca tcattatgag ttttctcatt tataaatata
     2401 agttatatat actatttgac cgaaattata ataagacctt ttaaaaatca gaaaccttca
     2461 aagaaattat ccttgaaggt taaatattac tgcttgtggt tataattaga atttaaaata
     2521 tgatcttttt tctccctgtt cccataattt tttaagttcg tgataatttt tttgtaactt
     2581 atccaacccc tctttaatac tttcagtatc aattacgaca ggtacaaaat gctttttaac
     2641 tctttttatg tttggaccaa attgaaggtt tactcccacc tgcacatctt cttctttaag
     2701 gatctttaca atactcttag ccttcttggg atcagcatgt ttttcttctt caattgaatt
     2761 attggctatc tttaaaataa attcagcttt atcttcatca acttcataaa tataataata
     2821 atcagcatca ccaaaatgcc tattcacaaa tctttctccc tcatctgtag ctattgcaac
     2881 ttttaaactc aatttaactc ctcctactgt taaaatagta atatttcaaa tatttattat
     2941 taaatcttat agtgccatta aaaagtatat attatataat aactaaagtc cagttctaac
     3001 agcaagataa aaaggagcag ttaaactgcc ccttttatct taatattata ttttactttt
     3061 tatatctaat ctaacataac atccttaacc agccattcac cattaggttt aattattaga
     3121 ttgcttacat tatttctggt tgcagctact acagtttgat ggtataaagg aatccagact
     3181 gcctgttcta ccaggtattt attggcttca tgcagcagtc ttaatctttc ttcctgatct
     3241 actgtggtcc tggtagcggt aatcatttca tcagcacctt catagaagga ccgattggct
     3301 cctcccaaat tatctgaatg gaggttaggg taaatcaatt cactaccatc accagttaaa
     3361 tttgaccaac cgagtaagaa taggtcctgt tctccctttt gggttgcaga tagataacta
     3421 ccccactcga gtaatttaac attggcatct atgccgatct ttttcaattc agcctgcagt
     3481 atctgggcca tacgttggta atatgtgtca taagaagttg accagattgt ggtttcaaat
     3541 ccatctggat aaccggcttc ggccagtagt tccctggctt tttctaggtc atattcaaat
     3601 ccgtactctt caatctcttc tttatatcca aaaaccttgg ggccaataat accgtgggat
     3661 gcatagccta agccattcag ggttgaaact aaggcttcct ggttaactgc atgggcaata
     3721 gcctgcctga gttttaaatt attgaagatt tcctttttaa agttaaaacc aaaatacctt
     3781 atcggactac cctgaacaag ttttacctca atatctgaat tattttttag tcgatctaaa
     3841 tgttcgggtg gtatagtatc tagaaaatct acttcccccg tttccaacat ggcaattcta
     3901 gtggcagctt ccggcactat tttgaatacc agtttatcaa cattaccggt ttccccccag
     3961 taatcctcat ttttctttaa ggtaatatgg tcacctgaca cccattcatc gactacataa
     4021 ggtccggttc ctgcttcatt ctgcattaaa tcaccgtatt tttcgactga agtcggactt
     4081 acgatagaag cattactatg ggtcaaggtt gctaagaaag gaccataagg atacttggtc
     4141 ttcatcacaa cagtgtgttc atcgcgaact tctattgact caatgggttc aaccaaactg
     4201 gcccggggag aagctacctc gggatctccg atcctttcaa atgtaaattt aactgcttca
     4261 gcattgaagg gggtaccatc atggaacgta actccctctc ttaagtggat aatccaggtt
     4321 tttgaatctg gattctcata acttactgcc agactgggtt ctaattcatt tgtttccgga
     4381 tttctgacaa ataatgtgtc atagatctgt gctaggacat cagaagttac attatcattt
     4441 gttaataccg gtgaaagacc aacaatttct gaactggtgg caaaattaag taccttttcc
     4501 tgagctccag ccccaaaact aaataataat agggcgagtg tgaataaaac aataatcttt
     4561 ttaaacatat caatactcct ctctactctc tattttaatt ttttatcctg cattttcaac
     4621 tagatatagc catttctaac taataatcta ctcctcgcta atcgcctcct ttttaacgtt
     4681 atcttaagtt actaggatca aatgcatctc ttaaaccatc accaactaag ttaaaactca
     4741 agaccaacat cattatggcc agccccggca ctattatcat gtgaggggca ttgtatataa
     4801 aagacctgcc tgcactcacc atggctcccc attcgggtaa aggtggttga gcacccaggc
     4861 cgagaaaact taaagctgcc gctgacaata tggcagaagc tattcttaag gtagcataaa
     4921 cgattatagg acctatacaa ttcggcaaga tgtgctttat taagattata ggatctttta
     4981 ctcccagcgc ctctgctgcc attacatact cgtactcttt aatctgtaaa acctggcttc
     5041 ttacaagcct ggtaaaggtc gggatcgacc agatactaat cgctatgatt acatttataa
     5101 gtcccggtcc taacatagca ataatagcca gagctaatag tacacctgga aaagataaca
     5161 ttatgtcgat taacctcatt attatgttat ccagtttctt ataatatccc gcaataagac
     5221 cgagggtaac cccgataaaa agcccgaata aaacagaagt aagaccaact accagtgata
     5281 ttctagatcc aaagatcaag cgacttaata tatcacggcc gaagttgtcg gttcctagta
     5341 tatgtccttc tgttcctacc ggctgagtca tcttcattaa attctgttca taggggtcat
     5401 aaggtgctaa aaagggggca aaaatggcga taaacacttg taacaatatt aaaaatgaac
     5461 ctaataaggc cattttattc cttttaaact ttgaccagcg tttactctgt atacttatct
     5521 cttttttttc tctcttctct tttttgtcct tcacaatact cactcctcac taactataac
     5581 tgatgcgtgg gtcaagaaaa gcatataaaa tatcaactat taaattgata aaaacaaagg
     5641 taaaggcaat tgttaagaca caaccctgaa caaccggaaa gtctcttgta ttgatggccc
     5701 tcaccgctaa cctgccaatt ccatttatac caaatactga ttccgtgata acagtcccgg
     5761 ctaaaaggcg gccgaagtta agaccaataa cagttattac ggggataaaa gcattcttta
     5821 agatatgttt aaatacgata cgtttacctt taactccttt ggctttagct gtacgtatat
     5881 aatcattctg taaaatatct aaaatggaag acctggtcat tcttgctatc aaagcagctg
     5941 aatatacgcc tagagtaata gcaggcagga tcaaatgtct tagtccatca atagtccacc
     6001 attctgccgg cagtccactc accggcagta tgcccaattt aactgaaaat atgtacatta
     6061 agaccaaacc tatccagaaa gtgggaaaag aaataccgat aagagctaca aacatgctgg
     6121 tataatcaaa tatagaatac tgcttcactc ctgagataat tccggtaatt aatcccacaa
     6181 gggtagcaat tatcatactg gctgttgcta aaactaaggt attgggatag cgggttaaaa
     6241 gcatctcgct tactggtttt tgcgactgca cggattgtcc gagatcaaag gtcaaaatcc
     6301 cttttagaaa ggtaaaatag cggatatgaa tgggtttatc cagatttaat ttggtcctca
     6361 aattctcaat atctgactga gaagcttggg ggcctgccat gatggcagct ggatcacccg
     6421 gggctaggac tgtcattaga aaggccagaa tagatacacc tattaagact ggcactatta
     6481 atattaatct tcttgctata taagaaatca tctgactact cccttctaat aaatgcttgt
     6541 gataatttca gatacatata gaatattagt atattattca acaatgtaat gaaaataata
     6601 atattaatgc ttataagtat gttaaatata aatttttata ttacgataat aacatagaag
     6661 aaattagaag tcaacaccaa ctaaggtgtt tactgtaagt ttaaattttc aaaaaatttc
     6721 aatagataaa atttttaatt taattttttt gtatttttta atattaccta tcattcctat
     6781 aataataacg atgatcttca aaatatttta aataaattaa ttatagcttt tatttaatgc
     6841 tattaattta ctctataact tgacaaaccc ccctagcttt tttataatct tgatggtttc
     6901 gtcaatgatg cattttatta ctcaacctcg aggaggtaat agtaaataat gattgatgaa
     6961 aatctaggag aagaaattaa ggaatggatg aaagaacagg tagacaaaag aaacgctgaa
     7021 ggagttgttg ttggactaag tggtggtatt gattcttccc tggtagcggc cctctgtaaa
     7081 gaggcttttg gagatgattt actgggagta ttactaccag ccagtggaac tatctctaaa
     7141 gacattgaat acgctaatat gctagcagaa gaatttgaca ttgaaaccat gacagttaat
     7201 ttagaagaaa taaatgataa aatagaagaa acctttcata acattgatat cacaaaagca
     7261 gaaagagaat actgtccaca atctaaaata gcttccagtg acccggctga acaaaacatt
     7321 cagacccgtt taagaatgtt aaccctttat tatatagcag aaaaattaaa ctatgtagtt
     7381 atgggtacca gtaataaaag tgaaatctta tccggttatt atacattaca tggtgatgaa
     7441 gcaactgata tgagacctct cggagactta accaaaaccc aagtttggga attatctgaa
     7501 caggttggcg ttccagaaga aattataaat cgtgacccca gtggaggtct aatcggaagt
     7561 gaaaccgacc gtgatgaaaa agatcttggt gttgattatt caaccttcga taaaatttat
     7621 gaggctttag aaaacgatgg agatttaagt caatttgatg aagatgatgt ggctagaacc
     7681 aaagaattaa ttgaagctgc tgcagataag gaaaaaactc ctacttttaa attagattaa
     7741 tatgtaagtt aaaatgccgg gcattgcccg gcattctatc tttaatatga gtattaaatt
     7801 ccaacatgaa atcgaattta aattttaaac acatttataa ttaatattat atttaaaatt
     7861 agttttcttc actttcatat agatgactgt aataactatc agcttgataa acagccacta
     7921 aaggattatg ccctaatact ctatctttta ctgccaaaac agtggtaggc gctttggaat
     7981 gtttgaaaaa taggctgtcg tgaccaacac acagccctaa taccacatta aaatctgttt
     8041 cacattcagc caataagttt gcctgaccaa taggattaca cccggcttca aagccttccg
     8101 gtgaaaatct atcttcaggc ggtatatcta agtctgcttt atcaatactg ccgcagctgc
     8161 agaccgcaga atttacttcc aatcctcttc cttcaaaata tttggttgct atcttggcct
     8221 ctttttctaa acctatacaa aaggcaatac caatgttttt aaaatcagcc gctattgcat
     8281 attcagcaat ttcttttaag cggggccatt ttttataacc tgtggtctca atatgagcag
     8341 ctgttttata taatttcatg ttttcttctt tttcatattt cttcaatgca tctgttaacc
     8401 aatttccatt accctgtaga caccatttag gataatcatt agaatcaata ctgcatgtct
     8461 ttttatcaca ttttgcacaa attgaatgca tcttattttt atcgaacatt ttaaccctcc
     8521 atttttattt gtataacatt tatatttaat ttattgtgaa aaataaaaag gtattattag
     8581 ataatttatt aaagattaaa ttagattatc ctttaaattt tagtaaacta aaaatccatt
     8641 aggaataaaa aaattatcat tactataatt tcttatattt ttacttgaaa tttactattt
     8701 aagaattata aatctctcat tataaattta aatataggtt tttttataat ataattcatt
     8761 gatatataaa aaaaccttga gagcatatct catctctcaa ggtataataa attaaaacaa
     8821 tttaaaatag tatatcttat tgattgggac caaagttggg atcaggcatc tttactaccg
     8881 catgtacaat tacataaatc tcacctgaca aatcatcttc aatataatat tggcctggag
     8941 caacagtatc tattgtcttt ttaccacggg tttgttgtgg aaacatatct tttcctgcat
     9001 atatatggta ttcttcaatc tcatatggcc aatctaaatt aaatttaaca ttcttaatgt
     9061 ctccatcctc atattcaaat tttacagtac ctacattttc tcctttatca ggatcacaaa
     9121 atgctgctcc tgtatataaa ggccatacat attcattttc actaggtacg attggatttg
     9181 tccaacccca ttggctaaat tgataattaa ggaagcactt agcggattct cccatggcat
     9241 aggctgtttc atcaatatag cattgaacat tgactttgag agatgcactg gatgattgtt
     9301 gagtttcaat tatagtagca gtgttgtcat attgataatc accacataaa cttcctccat
     9361 agtcttccca tttaaaacat ccatcataca taaatgtgtg atttgaaggt gcagtaactg
     9421 ttccaagatt aacttttccg aaaagttcgc ttatatcttt gatgtttacg gtttcattaa
     9481 tcacattatc ggggtaaccc caaacaatat cttcacttgt tgagtattct cttaataact
     9541 cggttgttac tgtaacctca ttaatacctt caacatatcc atcttcatca taagttccaa
     9601 ctaactcttc cccaa
//
LOCUS       DATGCJ010000142         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_510413, whole genome shotgun sequence.
ACCESSION   DATGCJ010000142 DATGCJ010000000
VERSION     DATGCJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510413"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..391
                     /gene="gatC"
                     /locus_tag="VKN64_07210"
     CDS             98..391
                     /gene="gatC"
                     /locus_tag="VKN64_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMA60065.1"
                     /translation="MIDKDEVRYIADLASLKLSEEEVEKFSKQLSDILDYVEKLDELD
                     TEDIVPTAYTIPVKNVMREDKVEESLELDKVLQNAPEQKDNQFRVPKIMDEDN"
     gene            424..1866
                     /gene="gatA"
                     /locus_tag="VKN64_07215"
     CDS             424..1866
                     /gene="gatA"
                     /locus_tag="VKN64_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMA60066.1"
                     /translation="MKLYKRSLTDLKKMLDEKRVKLNDIHESFSYRIGSVDSKVKAFV
                     NTNKDLEIDKLKEEDYEDTYLKGLPVAIKDNISTEGIETTCSSRMLEDYNPPFDATVV
                     KKIQKAGGIITGKTNMDEYAMGSSTENSAFFPTVNPWGENRVPGGSSGGSAAAVAAGM
                     APVALGSDTGGSIRQPASFCGVVGMKPTYGAVSRYGLVAFASSLDQIGPLARNVDDIA
                     MMMNVITGSDDNDSTCVDREYPDFTDYLKNDVEGMKIGIPNQYFEIDFDKEVRESVMK
                     AVEKLEDAGATVKRVDVTGAEYALAAYYIIATAEASSNLARYDGVRYGLRSENANNLR
                     DMFTETRQQGFGDEVKRRIMLGTYVLSAGYYDDFYLKAQKVRTLIKNDFDEIFEEYDF
                     IVSPTTPTTAFKIGEKMDPLEMYAADIFTVPVNIAGIPAISLPCGFDQKGLPIGIQLM
                     GPHFEEEKLIQAAYSLEKILNLDIKYPALK"
     gene            1883..3328
                     /gene="gatB"
                     /locus_tag="VKN64_07220"
     CDS             1883..3328
                     /gene="gatB"
                     /locus_tag="VKN64_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMA60067.1"
                     /translation="MDKNYETVIGLEVHVQLDTDSKIFCSCSTEFGSEPNTNTCPVCL
                     GLPGTLPVLNKKAVDYLVMTGLALNCEIATYSKFDRKNYFYPDLPKAYQISQYDLPIA
                     KDGFIEVETEEGTHKIGITRIHLEEDAGKLIHEGSIDRSSGSLVDYNRTGVPLAEIVS
                     EPDIRTPEQAREYLNYLKKTLEYLGVSDCNMEEGSLRCDANISLRPRGDKEFGTKVEL
                     KNMNSFKAIEKALAYEQKRQRQMIEAEETIVQETRTWDEDKSKTISMRGKEEANDYRY
                     FPEPDLVPLEINESWENEIKEKLPELPAVRKKRFIEDYDLPEYDADVLTDSRELADLF
                     EECVSEYEDAKEVSNWIMGEFLRLVNEEKMEVEETKITGQLLGKMLKMMDEGVISSKI
                     AKTVFEEMFYTGKDPEDIVEEKGLKQISDEDKLEKLVEEIIEDNPDVIEDIRNGKDKA
                     IGYLVGQVMKETKGKANPQMVNKMFKEKINN"
     gene            3343..>4415
                     /locus_tag="VKN64_07225"
     CDS             3343..>4415
                     /locus_tag="VKN64_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60068.1"
                     /translation="MAEIASLTLVTGTEQTRQTLHKQLNEYITDSIPINSYAVDEGLT
                     SKIKDDLVILSSDILYKELLAADKLCEDCEVIIAKRTINYDAIDEIMFLDPDTDVLFV
                     NDIKETAYAGIEALKKLQIDHINLYPYYPDMTEEIKKAEVAITPGEVNKVPDFVEEVY
                     NIGPRIIDFTTILKILNRLDILEHKAGQFSRSYLNKIINIAKKFAQSANEVSNLNEHL
                     STVINSLNDALVVYNNKGYISVTNANFNDLFNLNSFNVNGKCIRDLIYSKNLLTFLMS
                     DKKDKKVFEIKNKNYVVSKFSLPESDFYTAVFKSEKDTMDDTKRINQEFINKGFVAKY
                     NFEDIIGESRANRKVKKVAKKLA"
BASE COUNT         1583 a          660 c          952 g         1220 t
ORIGIN      
        1 ttttatgggg attggtttaa gcctattact aaggattatg gtagcgggta atcttttaga
       61 tagatttaaa gatgaagaag ataaggagtg ataataagtg attgataaag atgaagttag
      121 gtatattgct gatctggcca gtcttaaact ttccgaggaa gaagtagaaa agttttccaa
      181 gcagttaagt gacatcttag actatgttga aaaacttgat gagttagata cggaagatat
      241 agtgcccaca gcttatacga ttccggtaaa aaatgttatg cgagaagata aagtagaaga
      301 atctcttgaa cttgataaag tattacagaa tgcacccgaa caaaaagata accagttcag
      361 agtaccaaaa ataatggatg aagataatta aggaggtgtc aatcccattt aagggaatac
      421 gatatgaaat tatataagag atcattaacc gatttaaaaa agatgttgga tgaaaaaaga
      481 gttaaattaa atgatattca cgagtctttt tcctaccgga ttggtagtgt ggattccaag
      541 gtaaaggctt ttgtcaatac aaataaagat ttagagatag ataagcttaa ggaagaggat
      601 tatgaagaca cttatttaaa aggactgcca gttgcaatta aggataatat ttcaacggaa
      661 ggaattgaaa caacctgttc ttcccggatg cttgaagact ataacccacc ctttgatgcg
      721 accgttgtaa aaaagataca aaaagcgggt ggcataataa cgggtaagac caatatggat
      781 gaatatgcga tgggttcctc aaccgagaac tccgcctttt tccccactgt aaacccctgg
      841 ggagaaaaca gggtaccggg tggttcaagt ggaggttctg ccgccgctgt agcggctgga
      901 atggccccgg ttgccttagg ttctgatacc ggtggttcta ttagacagcc cgcttctttc
      961 tgtggagttg ttggtatgaa acccacctat ggggcggttt ccaggtatgg tttggttgcc
     1021 tttgcttctt ccctggacca aattggacct ttagcccgta atgttgatga tatagccatg
     1081 atgatgaatg taataaccgg tagtgatgat aatgattcca cctgtgttga tagggaatat
     1141 cccgatttca cagattatct aaaaaatgat gtagagggta tgaaaatagg tattcctaat
     1201 caatattttg agattgattt tgataaagaa gtaagagaat ctgtcatgaa ggcggtagaa
     1261 aaacttgaag acgctggtgc taccgttaaa agagtagatg tgacaggagc tgaatatgca
     1321 ctagcagctt actatataat agcaacggct gaggccagct ctaacctggc tagatatgat
     1381 ggagttcgct atggtttgag gtctgaaaat gccaataatc ttagagatat gttcactgaa
     1441 accagacaac agggttttgg cgacgaagtt aaaagacgta ttatgcttgg tacctatgta
     1501 ttaagtgctg gttattatga cgacttttat ctaaaagctc aaaaggtaag aacccttata
     1561 aaaaatgatt ttgatgaaat ttttgaggag tatgatttta tagtatcacc aactactccc
     1621 accacagcct ttaaaattgg tgaaaagatg gatcccttag aaatgtatgc ggctgatatc
     1681 tttacagtac cagtaaatat tgccggtatt ccagcaatat cgttaccatg tggatttgac
     1741 cagaaaggtc ttcccatcgg tattcaatta atgggcccac attttgaaga ggaaaaacta
     1801 atacaggctg cttattcgtt agaaaagata ttgaatttgg atattaaata tccagctttg
     1861 aaatagagat ggaggtgtaa taatggataa aaattatgaa acagtgattg gactggaagt
     1921 acacgtccaa ttagatactg actctaaaat attttgttcc tgtagtacag aatttggtag
     1981 tgaaccaaat accaatacct gtccggtatg tttgggattg cccgggacac tgcctgtatt
     2041 aaataaaaaa gcagtggatt atctagtgat gaccggttta gccttaaact gtgaaattgc
     2101 tacctatagc aaatttgatc gcaaaaacta cttctatcct gatctaccaa aggcttatca
     2161 gatttctcag tatgacctac cgatagccaa ggatggattt attgaggttg aaaccgaaga
     2221 aggaactcat aaaataggta ttactagaat ccacctggaa gaagatgccg gtaaactaat
     2281 ccatgaaggt agtattgata gatcatcggg tagtttggtt gattacaata gaacgggtgt
     2341 acccctagct gaaatagtga gtgagcctga tattagaact cctgaacagg caagggaata
     2401 tttaaattac ttaaagaaaa cattagaata tctaggtgta tccgactgta atatggaaga
     2461 gggctcttta agatgtgatg caaatatttc actcaggccc cggggtgaca aagaattcgg
     2521 aaccaaggta gaactaaaaa acatgaactc ttttaaagcg attgaaaaag cacttgccta
     2581 tgaacaaaag aggcagagac agatgataga agcagaagaa accatagtcc aggaaaccag
     2641 aacctgggat gaggataaaa gtaagacaat ctccatgaga ggtaaagaag aagcaaatga
     2701 ttaccgctat ttcccagaac cagatttagt accactagaa atcaatgaaa gctgggaaaa
     2761 tgagattaaa gagaaactac ccgagctgcc agctgtccgt aaaaaacgtt ttattgaaga
     2821 ttatgaccta cctgaatatg atgcagatgt cttaacagac agccgggaat tagctgatct
     2881 ctttgaagaa tgtgtttcag aatatgagga tgccaaggaa gtcagcaact ggattatggg
     2941 agagtttcta agactggtta atgaagaaaa gatggaagtg gaagagacca aaattaccgg
     3001 tcagttgctt ggtaaaatgc tgaagatgat ggatgaaggt gtgataagtt ccaaaatagc
     3061 taaaactgtt tttgaagaga tgttctatac aggtaaagac cccgaagata ttgtagaaga
     3121 aaaaggtcta aaacagatta gtgatgaaga taagttagaa aaactggtag aagaaatcat
     3181 agaagataat ccggatgtaa ttgaagatat aagaaatggc aaagataagg cgatcggtta
     3241 tctcgtaggt caggttatga aagaaaccaa aggtaaagca aacccacaga tggttaataa
     3301 gatgtttaaa gaaaagatta ataattaaag gggcatataa aaatggcaga gattgcaagt
     3361 ttaacccttg tgaccgggac cgaacagacc cgacagaccc ttcataaaca gctgaatgaa
     3421 tatattactg acagcattcc cataaatagt tatgcagttg atgaagggct gacaagtaaa
     3481 ataaaagatg accttgttat cctttcaagc gatatattat ataaagaact tcttgctgcc
     3541 gataaactct gtgaagattg tgaagttatc atagccaaaa ggacaatcaa ttatgatgct
     3601 atagatgaaa taatgttttt agatcccgat acagatgtat tgtttgtaaa tgatatcaag
     3661 gagactgctt atgcaggtat agaggcctta aaaaaactgc agatcgatca cataaacttg
     3721 tatccctatt atcctgatat gacagaggaa attaaaaaag ccgaggtagc tattacaccg
     3781 ggggaagtta ataaggtacc tgattttgtt gaagaggtat ataatatagg tccccgtatt
     3841 atcgacttca ctactatttt aaaaatatta aacagactcg atattttaga acataaagcc
     3901 ggccagtttt cccggtctta tctaaacaag attattaata ttgccaaaaa gtttgcacaa
     3961 tctgccaatg aagtatccaa tttaaacgaa catcttagta cggtcattaa tagtttaaat
     4021 gatgctttag ttgtctataa taacaaaggc tatataagtg ttaccaatgc taattttaat
     4081 gacctgttta acttaaattc ttttaatgtg aacgggaagt gtattagaga tttaatatac
     4141 agtaaaaatt tacttacatt tttgatgtct gataaaaagg ataagaaagt ttttgaaata
     4201 aaaaataaaa actatgtagt aagtaaattt tcattacctg aatcagactt ttatactgcg
     4261 gtatttaaaa gcgaaaaaga tacaatggat gacaccaaac gtattaatca ggagtttata
     4321 aataaaggct ttgtagccaa gtataatttt gaagatatta taggagaaag cagggccaac
     4381 agaaaagtaa aaaaagtagc taaaaaactg gcaaa
//
LOCUS       DATGCJ010000143         8200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_375940, whole genome shotgun sequence.
ACCESSION   DATGCJ010000143 DATGCJ010000000
VERSION     DATGCJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375940"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VKN64_07230"
     CDS             <1..1088
                     /locus_tag="VKN64_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMA60069.1"
                     /translation="GRKMSKSLGNGIDPIEVIEEYGADALRFTLITGNTPGNDMRFRE
                     ERLEASRNFANKIWNASRFILMNLEDFDYNSIKEEDLKLNLADRWMLSRTNEVTQKID
                     DAMERYSFGEMSKSLYDFIWNEYCDWYIEILKERLYQDKDLQAKKTAQYMGLKILGNI
                     LKMLHPVMPMITEEIWQKLPISDKSIMVTSWPSYSESEIDKTAEEKMDLIMSVIKSVR
                     NIRNEMKVDPGRKIEAILLAGSSKEAILQEGESYLKNLANIEDLTITESLEERPQPAS
                     TAIVEGVEVILPLEGMVDIEKELDRLQKELQDVEYEIERAKGKLNNEGFVNNAPDELV
                     EGEREKVKEYQEKKDKLLDRIEQLKLN"
     regulatory      1147..1248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1417..2709
                     /gene="purD"
                     /locus_tag="VKN64_07235"
     CDS             1417..2709
                     /gene="purD"
                     /locus_tag="VKN64_07235"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMA60070.1"
                     /translation="MENILIVGSGGREHALCKKLKESSRVEKIYVLPGNAGTSDIAEN
                     VDIDPLNFKEITDFAKAKEIDMTFVGPEKPLVEGIVDYFNEEGLKIFGPNKQAARLEG
                     SKVYAKRVMEDCGIPTAKFSTFENSDEALNFIKDCDFPVVVKAEGLAAGKGVLIVENQ
                     QEAEAAITKIMEDRYFGEAGKRVLIEEFLKGEEATIMVFSDGKTYYPMVSAQDHKQVG
                     EGDTGPNTGGMGAYAPTPLVDEKVKNKVDKQIITPLFTYLNNHGIEFKGVLFIGLMIN
                     KGQPKVLEFNVRFGDPEAQVVLPLLENDLVEIADKIDEEKLNEINLKWQDKKTMAVIM
                     ASGGYPIDYDKGKKINGLEKFSANSNPYIIQAGTKYVDNEIYTDGGRVLALVSVGDTY
                     QEVYDLCYEKISAIDFEKAYYRKDIGRRIKGVKLLKDE"
     gene            2702..3220
                     /gene="purE"
                     /locus_tag="VKN64_07240"
     CDS             2702..3220
                     /gene="purE"
                     /locus_tag="VKN64_07240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMA60071.1"
                     /translation="MNKKIGIMMGSDSDLPVMKSACQVLEKLDVEYELTVISAHRTPE
                     RAREYALKAEENGIGVIITAAGKAAHLPGVIAASTIVPVIGVPIRTSTLGGADSLYSM
                     VQMPGGIPVATVALNGAKNAALLAVQMLATGDKEFREKLIQYRKEMQEDVNNAAAELE
                     EKGYKKYLEEHF"
     gene            3239..4636
                     /locus_tag="VKN64_07245"
     CDS             3239..4636
                     /locus_tag="VKN64_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMA60072.1"
                     /translation="MINRNIFENINPFDHRYSYKKEVFDEIKNYLSESANINYQLKVE
                     LALVKSLNKMGICSQQVVEEVRQAVKDIKAAEAYEEERKTKHNIRALVNLIQEKVSDE
                     AKPFIHLTATSYDIVDTANSLRYKEVTEDIILPQLAKLINRLIQLAEQEKDTVQIGRT
                     HGQHAVPITFGYVLAGYVERLGLRYKEVQQKAAGLKGKFSGACGTYNASSLFFKDPVQ
                     FEKTVMKELNIRAGDFSSQIVIAEYMTDFIHSIISTFGILANFSDDMRNLQRTEINEV
                     GEFFSKDQVGSSTMPHKRNPINYENIKSMWKRTMPQMNTIYMDQISEHQRDLTNSASS
                     RFIPDLLTGFILSVQRLIKVLDKFGIDSKKMQENFDLHREMIIAEPLYIILASQGHPD
                     AHEAVRRLTLQAEESEKTPRELFYLKDEFAEYREKMTDYQKEMIDNPTKYTGVSRERT
                     EYITQKWAKFLKEER"
     gene            4639..5358
                     /gene="purC"
                     /locus_tag="VKN64_07250"
     CDS             4639..5358
                     /gene="purC"
                     /locus_tag="VKN64_07250"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMA60073.1"
                     /translation="MEKGKLLYEGKAKKIYETDNPEQLIVHYKDDATAFDGKKKGQIE
                     AKGKYNLEISNIFFMLLEDHDVKTHLIRQVNETDLLVKKVDIIPIEVVLRNVAAGSLA
                     DRLGLEEGTELNRPILEYYYKSDELGDPLINRYHISERGLATEGQLKLLDILGFKINK
                     ILKEYLEEKDIKLIDFKLEFGVDSNDNILLADEISPDTCRFWDMKTDKKLDKDRFRRD
                     LGEVEDAYKEILRRLKKGVVS"
     gene            5358..5618
                     /gene="purS"
                     /locus_tag="VKN64_07255"
     CDS             5358..5618
                     /gene="purS"
                     /locus_tag="VKN64_07255"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMA60074.1"
                     /translation="MDWEVKIETKLKEGILDPQGKAVKTGLKQLGYDNVESVHVGKYM
                     ELILDDIPDKEKAAEMVVDMCEKLLANPVIEDYKYELTEVEG"
     gene            5620..6321
                     /gene="purQ"
                     /locus_tag="VKN64_07260"
     CDS             5620..6321
                     /gene="purQ"
                     /locus_tag="VKN64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMA60075.1"
                     /translation="MKAGVVTFPGSNCDQDVFNVLDNIFDFESDMIWHKEKSLNGYDL
                     IVLPGGFSYGDYLRSGAIARFANIMDSVIDYANQGGLVLGICNGFQILLESDLLPGAM
                     KINDSLKFVCKNVQLQVVNNETPFTNQYENSEIIEMPVAHGEGNYYIDDKRLKNLESN
                     NQIVLKYKDKSENPNGSIENIAGICNKERNIFGLMPHPERCAEDIISPGRSDGYRMFS
                     SIMEEIKVRGEKIAR"
     gene            6311..>8200
                     /gene="purL"
                     /locus_tag="VKN64_07265"
     CDS             6311..>8200
                     /gene="purL"
                     /locus_tag="VKN64_07265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HMA60076.1"
                     /translation="MPDKPWEKEGLSENEYNMIVDLLDRKPTETELGIFGVMWSEHCS
                     YKNSKLILKTLPTEGDCVLQGPGENAGIIDIGDKQAISFKIESHNHPSAVEPFQGAAT
                     GVGGIIRDIFTMGARPIATLNSLRFGDLDKDRVKFLFKGVVAGISSYGNSIGIPTVGG
                     EVFFSDAYEGNPLVNVMSVGLLDHKDIATATARGIGNSVMVVGAATGRDGIQGASFAS
                     DELTEDSDEDRPAVQVGDPFMEKLLLEASLELIKSDSIIGIQDMGAAGLTSSSAEMAS
                     RGNSGIEIDIAEVPTREKNMTPYEIMLSESQERMLVVPKKGQEEQVKEIFDKWGLHAE
                     VVGEVTGDGMLRILNGEEKVAEISARILADEAPEYFRKAVRPKYIDDINKITANDIAG
                     DDYNQDLLALLSSPTIANKNWVYKQYDHMVMTNTVVGPGSDAAVIRIKGKDKAVALTT
                     DNNGRYCYLDPLKGGKIAVAEAARNIVCSGGRPLAITDGLNFGNPMDPEIYWQFKNSV
                     EGLTKACKELETPVIGGNVSFYNESPKGAIYPTPIVGMVGLLDSIEERVTMHFKDEGD
                     LIYLLGRNKEELGASEYLALKEDKIDGYVPEIDLKLEKRVQRLVREAVKEGIIKSAHD
                     CSEGGL"
BASE COUNT         2857 a         1251 c         1901 g         2191 t
ORIGIN      
        1 agggccgaaa aatgagtaaa tcattgggga acggtataga tcccatagaa gttattgaag
       61 aatatggggc agatgcgctt aggtttaccc tgattacagg taatacccct ggtaatgaca
      121 tgcggtttag ggaagaaaga ctggaagcca gccgtaactt tgctaacaag atatggaacg
      181 cttcccgttt tatattaatg aacttagaag attttgatta taattcaatt aaagaagaag
      241 atttaaagtt aaatcttgct gatagatgga tgttaagcag aactaatgaa gtaacacaaa
      301 agatagatga tgcgatggaa agatatagtt tcggtgaaat gagtaagagc ctttatgact
      361 ttatctggaa tgaatactgt gattggtata ttgagatttt aaaagagaga ctctatcagg
      421 ataaggattt acaggctaaa aagaccgctc agtatatggg cttaaagata ctcggtaata
      481 tcttaaaaat gcttcatcct gtaatgccga tgatcacgga agaaatctgg cagaagttac
      541 ctatttcaga taaaagcatc atggtgacaa gctggccgag ctatagcgaa agtgaaattg
      601 ataaaacagc tgaagaaaag atggacctaa ttatgagtgt aataaaatca gttagaaata
      661 ttagaaatga aatgaaagtt gatcccggtc gtaaaatcga agcaattctc ttggctggtt
      721 cgtccaaaga agcgatctta caagaaggag aaagttatct caagaatctg gctaatatag
      781 aagacttaac cattactgaa agtttagaag aaagaccaca gcctgcttct accgctattg
      841 tagaaggggt agaggtaatc ctaccactgg aaggtatggt tgatatcgag aaagaattgg
      901 acaggctgca aaaagaacta caggatgttg aatacgaaat agagcgggcc aagggtaaac
      961 tgaacaatga aggatttgtt aataatgccc cagacgagtt agttgaagga gaaagagaaa
     1021 aagtaaaaga gtaccaggag aaaaaggata aattactgga tcgtatcgaa cagttaaaat
     1081 taaattaaaa atagtattga cttaaaaatt atattctgtt attataagtg cataaattag
     1141 tgagaaaaat taaatatttt tgtacctcat ataattccgg taatagggtc cggaagttca
     1201 taccaggtag ccgataacta tctgactatg agggaagtgt acctaggtgg ccgcattttt
     1261 aaaaatggct ggttcgagcg gtacagataa cttatacacc gaagggataa aagcccaggc
     1321 gggtaggttc tactcttagt taatagtagt aggcactacc cgtctgggtt tttttatttt
     1381 ttataaaatt tttacaaatc aggagggata ttttttgtgg aaaacatctt aattgtcggc
     1441 agtggtggca gagaacatgc tttatgtaag aaattgaaag agagcagtcg agtagaaaaa
     1501 atttatgttc ttcccggtaa cgcaggtacc agtgatattg cagaaaatgt agacatcgac
     1561 ccattaaatt ttaaagaaat aacagatttt gccaaagcta aagagattga tatgaccttt
     1621 gtggggcccg aaaaaccttt agtagaaggt atagtagatt attttaatga agaaggccta
     1681 aagattttcg gtcctaataa gcaggcggcc cgtttagagg gtagtaaggt ttatgctaaa
     1741 cgagttatgg aagattgtgg gataccaaca gcaaagtttt ctacttttga gaattcagat
     1801 gaggctttaa atttcattaa agattgtgac tttccagtag tagttaaagc agaaggattg
     1861 gcagctggta agggtgtgtt aatagttgaa aatcaacagg aagctgaggc tgctatcact
     1921 aagattatgg aggatagata ttttggtgag gccggcaaaa gagttctgat agaagagttt
     1981 cttaaaggag aagaagccac aataatggtc ttttctgatg ggaaaacata ttatccgatg
     2041 gtgtccgccc aggaccataa acaggtaggt gaaggagata ctggtcctaa cacgggtggc
     2101 atgggagctt atgcccccac cccattggtg gatgaaaaag ttaaaaataa ggtcgataaa
     2161 cagatcatta cccccctatt tacttattta aacaaccatg gtattgaatt caaaggcgtc
     2221 ctgtttatcg gccttatgat caataaggga caaccaaaag tattggaatt taatgtgcgt
     2281 tttggtgatc cggaggctca ggtggtccta ccactacttg aaaatgatct agtggaaatt
     2341 gcggacaaaa ttgacgaaga gaaattaaat gaaattaatt tgaagtggca ggataaaaag
     2401 acaatggctg taattatggc ctccggcggt tatccgattg attatgataa gggtaaaaag
     2461 atcaatggtt tagaaaaatt ttcagctaat tcaaatccct atattattca agccggaact
     2521 aaatatgtag acaatgaaat ctatactgat ggaggtagag tattggcctt ggtatcagtg
     2581 ggtgatactt accaagaggt ttatgatttg tgttacgaga aaattagtgc aatagatttt
     2641 gaaaaagcct attatcgcaa agatatcggg agaagaataa aaggagtgaa attattaaaa
     2701 gatgaataaa aaaattggaa taatgatggg tagtgattct gacttacctg taatgaaaag
     2761 tgcctgccag gtactagaga aattagatgt agaatatgaa ttgactgtga tatcggcgca
     2821 cagaacaccc gaaagggcca gagaatacgc tttaaaggct gaggaaaatg gaattggtgt
     2881 cataattaca gcggccggca aagctgccca tttaccgggt gtaattgcgg ccagtacgat
     2941 tgtgcctgta atcggggtgc cgatcagaac ctctacttta ggtggggcag attcacttta
     3001 ctcaatggtt cagatgccgg gcggtatacc ggtcgcaacg gtggccttga atggtgcaaa
     3061 aaatgctgca ttattagctg ttcagatgtt agcaacaggt gataaagaat ttcgagaaaa
     3121 acttattcaa tacagaaaag agatgcagga agatgttaat aacgcggcag ctgaactaga
     3181 agaaaaaggt tataaaaaat atttagagga gcatttttaa ttactggagg taattaattt
     3241 gataaataga aatatatttg aaaatatcaa tccctttgat caccgctact cctataaaaa
     3301 agaagtattt gatgaaataa aaaattatct atcggaaagc gccaatatta actaccagct
     3361 taaagtcgag ttagctttag taaaaagctt aaataagatg ggaatatgtt ctcagcaggt
     3421 ggttgaggaa gttagacagg ctgttaagga tattaaagca gccgaggcct atgaggaaga
     3481 aagaaaaacc aaacataata ttagggcctt agtaaattta attcaagaga aagtaagcga
     3541 tgaagccaaa ccatttattc acctgactgc tacttcctat gatatagtgg atacagccaa
     3601 ttcacttcgc tataaagagg taacagaaga tattatctta ccccaattag ctaaactaat
     3661 taatagatta atacaattag ccgagcagga aaaagataca gttcagattg gccggaccca
     3721 cggtcagcat gcggtaccta ttaccttcgg ctatgtttta gccggttatg tagaaagatt
     3781 aggcttaaga tataaagaag tacaacaaaa ggcggctggt ttaaaaggta aattttcagg
     3841 tgcctgtggc acatataatg cttccagctt attttttaaa gatcctgttc aatttgaaaa
     3901 aactgtgatg aaagaattaa atattagggc aggagatttt tcttcccaga ttgttattgc
     3961 cgaatacatg accgatttta tccattctat catttctacc tttggcattt tggctaactt
     4021 cagtgatgat atgaggaatc tacagaggac cgagattaat gaagtaggcg agtttttcag
     4081 taaagaccag gtagggtctt ccacaatgcc ccataaaaga aatcccatta attatgagaa
     4141 tattaaaagt atgtggaaaa ggaccatgcc tcagatgaat accatctata tggatcagat
     4201 ctctgaacat cagcgcgatt taactaactc tgcctcgagc agatttattc ctgatcttct
     4261 gacaggcttc atcttatctg ttcagagatt aataaaggtc ttagataaat ttggaattga
     4321 cagtaaaaaa atgcaggaga actttgattt acaccgtgaa atgataatag cagaacctct
     4381 ctatattata ttagcttccc agggacatcc agacgctcat gaagcggtaa ggcgtttaac
     4441 cttacaggcc gaagagtctg agaaaacccc gcgggagctt ttttatttga aagatgaatt
     4501 tgccgagtat cgtgaaaaaa tgactgatta tcaaaaagag atgatagata accccaccaa
     4561 atataccggg gtatctagag aaagaacaga gtatatcacc caaaaatggg caaaattctt
     4621 aaaagaggag agataaaaat ggaaaaaggt aaattacttt atgaaggaaa ggcaaaaaag
     4681 atttatgaga cagataatcc ggagcagtta atcgtacatt ataaagatga tgcaacagct
     4741 tttgacggca aaaagaaagg acagattgag gcgaaaggta agtacaatct agaaatttcc
     4801 aatatatttt tcatgttgtt agaagaccat gatgtaaaaa cccatctgat aagacaggtc
     4861 aacgaaaccg acttattggt aaaaaaggta gatattattc caatcgaagt tgttttacgt
     4921 aatgtagcag ccggtagtct tgccgacaga ttaggtttag aagaaggcac tgagttaaat
     4981 agaccgatat tagaatatta ctataaaagt gatgaattag gagatccttt aattaacaga
     5041 taccatatta gtgaaagagg cctggctacc gaagggcagc ttaaactctt agatatttta
     5101 ggatttaaaa ttaacaagat attaaaagaa tacttagaag aaaaggacat taaacttatt
     5161 gacttcaaac tggaatttgg ggtagacagt aatgataata tcctgttagc tgatgaaata
     5221 tcacctgaca cctgccgttt ctgggatatg aaaacagata aaaagctaga taaagatcgt
     5281 ttcagaagag atttaggcga agtagaagac gcttacaaag agatcctccg ccgcttaaag
     5341 aaaggtgtgg ttagttaatg gactgggaag tcaagataga aacaaaacta aaagagggta
     5401 tattagaccc ccagggtaaa gccgtaaaaa caggcttaaa acaattaggg tatgataatg
     5461 tagagtcggt ccatgtcggt aaatatatgg aactcatcct ggatgatatc cctgataaag
     5521 aaaaagcagc cgagatggta gttgatatgt gtgaaaaact gctggctaat cccgtaatag
     5581 aagactataa atatgagtta acagaagtgg agggataaga tgaaggcagg agtagtaaca
     5641 tttcccggtt ctaactgtga tcaagatgtc tttaatgtat tagataatat atttgatttt
     5701 gagtcagata tgatctggca taaagaaaaa agtttaaatg gttatgattt gatcgtatta
     5761 cccggtggtt tttcttatgg agattacctg aggtcgggag cgatagctag atttgccaat
     5821 atcatggatt cggttattga ttatgctaat cagggcggct tggtcttggg catctgtaac
     5881 ggctttcaaa tacttttaga gtcagattta ttaccgggcg ccatgaagat taatgatagt
     5941 ttaaagttcg tctgtaaaaa tgtgcaactg caggtagtca ataatgaaac cccctttacc
     6001 aatcaatatg agaactcaga gataatcgag atgccggttg cacatggaga aggaaattac
     6061 tatattgatg ataaaagact taaaaattta gaaagtaaca atcaaattgt cttaaagtat
     6121 aaagataaat cagaaaatcc gaatggttct attgaaaaca ttgccggaat ctgcaataaa
     6181 gagagaaata tatttgggct tatgccccac ccggaaagat gtgcagagga tataatatca
     6241 ccgggccgta gtgacggata tcggatgttt tcatcaatta tggaagaaat caaagtaaga
     6301 ggtgagaaga ttgccagata aaccctggga aaaagaaggt ctatcagaaa atgaatataa
     6361 tatgatagtt gatttattag acagaaaacc aaccgaaacc gagttaggta tctttggagt
     6421 gatgtggtct gaacactgca gctataagaa ttcaaagtta atattaaaga cacttcccac
     6481 cgagggagat tgtgtactgc agggaccggg cgaaaacgcc ggtatcatag atataggtga
     6541 taagcaggca atttctttta agatagaaag ccacaaccac ccttcggccg ttgaaccctt
     6601 ccagggggca gctaccggtg taggaggaat aataagggac atatttacga tgggggctag
     6661 accgattgcc accttaaatt ccctccggtt tggagattta gataaagacc gcgttaaatt
     6721 cctttttaaa ggggtagtgg ccgggatttc aagttatgga aatagtattg gaatcccgac
     6781 cgtgggaggt gaagtatttt tctcagatgc ctatgaaggc aatccacttg ttaatgtaat
     6841 gagtgtgggt ttgctggacc ataaagatat cgcaactgct accgcccgtg ggattggcaa
     6901 ttctgtcatg gtggtcggag cggccaccgg cagagatggt attcagggag caagctttgc
     6961 ttcggatgaa ctaaccgaag attccgatga agaccgccca gctgttcagg tgggagaccc
     7021 ttttatggaa aaactccttt tagaagcaag tttagagttg attaaaagtg actccattat
     7081 tggtattcag gacatgggag cagccggttt aaccagttct tcagctgaaa tggccagccg
     7141 gggcaatagc ggtattgaga tcgatattgc cgaggtgcca acccgagaga aaaatatgac
     7201 cccttatgag atcatgctgt ccgaatctca ggagagaatg ctagtagtgc ctaaaaaagg
     7261 tcaagaagag caggttaaag agatctttga taagtggggt ttacatgccg aagtggttgg
     7321 tgaggtaacc ggagatggca tgttgaggat attaaatgga gaagaaaagg tagcagaaat
     7381 atctgccagg attttagctg atgaagcccc tgagtacttc agaaaagcgg taagacccaa
     7441 atatattgat gatatcaata aaataacagc taatgatatt gcaggtgatg actacaatca
     7501 ggacttgctt gcattactaa gctcacccac aattgctaat aagaactggg tatataaaca
     7561 atatgaccat atggtgatga ccaatacagt tgtcggtccg ggttctgatg cggccgtaat
     7621 aaggataaaa ggaaaagata aagcggtagc tttaacaaca gataataatg ggagatactg
     7681 ttatcttgac ccccttaaag gcggtaaaat agcagtggca gaagcagccc gtaatattgt
     7741 ctgttcaggc ggccgtcccc ttgccataac tgatggtctt aattttggta atccgatgga
     7801 tcccgaaata tactggcagt ttaaaaacag tgtggaaggc cttactaaag cctgtaaaga
     7861 attagaaaca cccgttatcg ggggaaatgt cagcttttat aatgagagcc cgaaaggtgc
     7921 gatctatccc accccaattg ttggtatggt cggtcttttg gactcaattg aagagagggt
     7981 gactatgcat tttaaagatg aaggagattt aatctattta ctgggcagaa acaaagaaga
     8041 attgggcgca agtgaatatc ttgccttaaa agaagataag atagatggat atgtaccgga
     8101 aatagattta aagttagaaa aaagagtgca aagattagta agagaggctg ttaaggaagg
     8161 tatcataaaa tcagcccatg attgttcaga aggtggtttg
//
LOCUS       DATGCJ010000144         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_644724, whole genome shotgun sequence.
ACCESSION   DATGCJ010000144 DATGCJ010000000
VERSION     DATGCJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644724"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VKN64_07270"
     CDS             <1..789
                     /locus_tag="VKN64_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA60077.1"
                     /translation="EFILENIGYEVERTTARDPIIHQGLTQGDIDVFIGSWMPQIMDM
                     RNKYKDEINYVTQNMTEGLYTMAVPQYVYEAGVKSHADLQKHADKFEKKMYVGPAGWA
                     SSKKMNKAIDEDIYGLGDWTAINSSQSALMAQVDKSINEEEWIVFVGWRPHWMNAEYN
                     LKYLEDPDRLWESPYSWVDTLTRKGFKEDYPQVYRFLQQFRVDVEDNDQWIYEIGYNE
                     RDEMEVAEEWVKNNILEVRRWLSMVETPAGEDAYQTLVNNLNLD"
     gene            1117..3096
                     /locus_tag="VKN64_07275"
     CDS             1117..3096
                     /locus_tag="VKN64_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60078.1"
                     /translation="MFKSIKSKMMIIIGLLVVVLVSTCSLWLYFQSRLILKESIMKNA
                     KQQAKGDAMAVNNWLNGIKGKMDEITRMPVFKEMNSKEYKEFLRDLANRYDYINNAFV
                     ANDRGIYYNQAGNQNSIAEKEYFKEAVKNRQTIFSSPVLDEATGKERIIVASPIESDN
                     KIVGVLSTTITLDYIQDIVKEMKINDSGHGWIIDQHKITIAHTNDAYLGNKILLEKGS
                     KELKEAANLMKEGNSDTAVYSMEGRKKVLAYSSINLTGWSIALTADLDTILAPLDIMK
                     KESILMGVIAALLGIVIAFLISRSIANPIKRITEQSKLIADGNLQQDFKDKIVERNDE
                     LGTLGQAIAEMKKNLRNILRKTSSIADELSSSSQELSASSEEISASAEQVSSAIQEVA
                     SGAEEQTAQIKETKDNIDKLTNKIKEVDKATNSMNKSSDNVTKNLVEGNKTIDKSIGM
                     VKEVKNEATTVAEKIDRLGDLSEKIGDIIELINGISAQTNLLALNAAIEAARAGEAGR
                     GFSVVADEIRELAEESANATERIADLIGDIQQGVKDTIGQMNKTTKAVESGVDSMRET
                     EQSFDNINEVSDNLQKMITSISESIKSMHTQSNEVEEAVVEIASVSQQASSNAEEVSA
                     SSEEQSATTREIVDASEELAEMAQRLTTSVEQFKL"
     gene            complement(3214..>3324)
                     /locus_tag="VKN64_07280"
     CDS             complement(3214..>3324)
                     /locus_tag="VKN64_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60079.1"
                     /translation="APWISLFSGLAIIITVMSFNLLGDALRDALDPKQIE"
BASE COUNT         1237 a          495 c          691 g          901 t
ORIGIN      
        1 gaatttattt tagaaaatat tggctatgaa gtagaaagga ctacggcgcg tgaccccata
       61 attcatcagg gtttaactca gggagatata gatgtgttta ttggttcctg gatgccgcag
      121 attatggata tgagaaataa gtataaagat gaaatcaatt atgttacaca gaacatgaca
      181 gaagggcttt atacgatggc agtaccccaa tatgtttatg aagctggggt aaaaagtcat
      241 gctgacttac agaaacatgc agataaattt gagaaaaaaa tgtatgtagg accagctgga
      301 tgggcctcat ccaaaaaaat gaataaggcg attgatgaag atatttacgg attgggcgac
      361 tggacagcaa ttaactccag tcagagtgcc ctgatggctc aggttgataa gtctataaat
      421 gaagaagaat ggatcgtttt tgtcggctgg cgtcctcact ggatgaacgc tgaatacaat
      481 ttaaaatact tagaagaccc agatagactt tgggaaagtc cttattcctg ggttgatacc
      541 ttaaccagaa aaggtttcaa agaagattac cctcaggtat atagattctt acagcagttt
      601 agagtagatg tagaagataa tgatcagtgg atctatgaga tcggatataa tgagagagat
      661 gagatggaag ttgctgaaga atgggttaaa aataatattc tcgaggtaag aagatggtta
      721 agtatggttg agactcccgc cggagaagat gcctatcaaa ccttagtaaa caatttaaat
      781 ttagattaat aaattaaaca ttaaaaatca atcccctgct ttgggcaggg gattttctgt
      841 ctttttatca tattttcaca aattatgttt gtcatatttt gccataaaat attagatata
      901 gaactttaag acaaaatatt ttaaaatctt tttttatacc cataaaagta taaatttctt
      961 ttattgttaa ataataccac tctatgatat agttatgatt gcttacaaaa tgaagatggg
     1021 gagggattta aaataaaatt agtttaatag taaagagagt tttaattaat taaataaaat
     1081 agaaatctat ctaataacca atggaggatg actttaatgt ttaaaagtat taaaagtaaa
     1141 atgatgatta tcatcggact attagttgta gtattggtta gcacctgttc actatggcta
     1201 tattttcaat ccaggctaat cttaaaagaa tccataatga aaaacgctaa acagcaggcc
     1261 aaaggagatg caatggcagt taataattgg cttaacggaa taaaaggtaa gatggatgaa
     1321 ataacaagga tgcccgtctt taaagagatg aatagtaaag agtataaaga atttttacgg
     1381 gatttagcaa atagatatga ttatataaac aatgcctttg tggccaatga tagaggaatt
     1441 tattacaatc aagcagggaa tcaaaatagt atagctgaaa aagaatattt taaagaggcg
     1501 gtaaagaata gacaaaccat attctccagt ccagttctag atgaagcgac cggcaaagaa
     1561 cgaattattg tagccagtcc tattgagagt gataataaga ttgtgggcgt tttatctacc
     1621 actataaccc ttgattatat tcaagatata gtaaaagaga tgaagattaa tgacagtgga
     1681 cacggctgga tcatagacca acataaaata accatcgccc ataccaatga tgcctatcta
     1741 ggtaataaaa ttttattaga aaaagggagc aaagaactaa aagaggctgc taacttaatg
     1801 aaagaaggaa attcagatac agcggtatat agtatggaag gacgaaaaaa ggttttagcc
     1861 tacagctcga ttaacttaac gggttggtca atagcattaa ccgcagacct tgatacaatt
     1921 ttggctcctt tagatatcat gaaaaaggaa agtattttaa tgggagtaat tgccgcttta
     1981 ctgggaattg ttatcgcctt tttaatctcg cggtcaattg cgaaccctat aaaaagaatt
     2041 actgaacagt ctaaactaat agcggacggt aatttacaac aggattttaa agacaaaatt
     2101 gttgagagaa atgacgaatt gggcacactc ggtcaagcaa tagccgagat gaaaaagaat
     2161 ctaagaaata ttctaagaaa aacttcttct atagcggatg agctttcttc ttccagccag
     2221 gaactttcag cctccagtga agaaatctct gcttcagctg aacaggtaag cagtgcaatt
     2281 caggaagttg cttcaggggc cgaggaacag accgctcaaa taaaagagac caaagataat
     2341 atcgataaac tgacaaataa gataaaagag gtcgataaag ctaccaattc tatgaataaa
     2401 tcctcagata atgtaacaaa aaatttagtt gaaggtaata aaacgataga taaatcaata
     2461 ggaatggtga aagaagtaaa aaatgaagct actacggttg ctgaaaagat agataggctg
     2521 ggagaccttt cggaaaaaat aggagatatt attgaattaa ttaatggaat ttccgcccaa
     2581 accaatctat tagccctaaa cgctgctatc gaagcagccc gggcaggtga ggccggccgc
     2641 ggctttagtg ttgtggcaga tgaaataaga gaattagcgg aagagtctgc aaatgcgacc
     2701 gaaagaatcg ccgacttaat tggagatatt cagcagggag taaaagatac aataggtcaa
     2761 atgaataaaa caaccaaagc tgtggaaagc ggtgtagatt caatgcggga gacagaacag
     2821 tcttttgata atataaatga ggtttccgac aatttacaaa aaatgattac cagtatttca
     2881 gaatctatca aaagcatgca tacccagagt aatgaagttg aagaagcagt tgttgaaatt
     2941 gcttctgtca gccagcaggc ttccagtaac gcggaagaag tgtctgcctc cagcgaagaa
     3001 cagagtgcta ccacccgaga aattgtagat gcctccgagg aactggctga gatggcccaa
     3061 aggctaacta cttccgtaga acaatttaaa ctttaaaaaa atagttaaaa agactaaaaa
     3121 aataatatta tagtaaaaaa gtgcaggccg gaaataaaat cctctcattt cttaatatcc
     3181 tgcacttttt tatatatttt atttaaaaga tgactattca atttgtttgg ggtctaaggc
     3241 atctcttaag gcatctccta ataagttgaa actcattact gtaatgataa ttgctagacc
     3301 tgaaaatagt gagatccagg gggc
//
LOCUS       DATGCJ010000145         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_62666, whole genome shotgun sequence.
ACCESSION   DATGCJ010000145 DATGCJ010000000
VERSION     DATGCJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62666"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1349
                     /locus_tag="VKN64_07285"
     CDS             99..1349
                     /locus_tag="VKN64_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HMA60080.1"
                     /translation="MPSIKLNISKKVFNKVYIPYLNDNTPTQIFFGGSSSGKSVFLAQ
                     RAVYDLAKGGRNYLILRKVQRDCRKSVWNEVIKVIKKFGLYNHISTNKTELTITFPNG
                     YQILFGGLDDSERIKSITPQKGVITDIWCEEATEFEEDDIKQLNKRLRGRAAAVKRLV
                     LSFNPIIKNHWIFKTYFNYWNGDNNRYQNDRISILKTTYRKNKFLTPDDIDRLLSEND
                     PYYRDVYIEGNWGVLGDIIFKNWEIKDISGMKNRVDRSCHGLDFGFSNDPAAVLKQYF
                     DKSSNNLYIFDEIYEKGLTDKKLADLTYKLIEFEEVICDSAEPKSIKHLNNHKLNARG
                     AKKGPGSIETRYRWYSGINIILSQKCVNIKRELQTHQWKKDKDGNSLKVPEDKNNHAI
                     DASMYGLSDYWNLEMQSWYTQDYV"
     gene            1361..>2697
                     /locus_tag="VKN64_07290"
     CDS             1361..>2697
                     /locus_tag="VKN64_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60081.1"
                     /translation="MALTGSEAVKMSFLEWIYSQDNFDRIKLYEENERYYDGDIDIKL
                     PANVKKYLAQEYGYTGNICRAVVDASVGFLSKEPVSIEIQEEEGKEENIKKAEEWIYE
                     KFRNNNLLIKDFIKALRIQAKKGEFAYKISRILDDNDEKVADYKITVLKPDICFPKWK
                     DESYEEMEYFAVEYVRYNSRKKEKEWFAQVIWPDLVKEYSRPLGSSKDQWKLINSWEN
                     KYGFINVEWVENKIDDSAWSESDITEDLKDLQNALNKSITDLVYTSDKEAFTQTFLLG
                     ANQPLDPETGEPQDIETGPGKLHIIPASADKIPKLGVIEAGNFQGLHDTLDKYLDLVS
                     IITKVPRIELNRMGSSDLGTGGVPTVIALRTIYQSFIGKTNEKSNLIKSGLENTAEKL
                     FKMAEVDDIDTKFEHQEFKTRIHIRSGLPSDDKEKMEVQEKEINNKIKSRETV"
BASE COUNT         1073 a          417 c          572 g          635 t
ORIGIN      
        1 atttttaact taaaaaacaa ctataactgg aaagataagc aggaaataga tcataaagga
       61 gatatagaaa tagatgtaac cctagaggat gaatagtatt gcccagtatt aagctgaata
      121 tatccaaaaa agtattcaac aaagtataca taccatattt aaacgataac acaccgacac
      181 agatattttt tggagggtcc agttcaggta aatcagtttt tctagcacag agagccgtct
      241 acgacctagc aaaaggcggt aggaactatc tcatattacg aaaagtccag agagactgcc
      301 gaaaatcagt ctggaatgaa gtaataaaag taataaaaaa atttggactg tataatcata
      361 tcagtactaa caagacagaa ttaacaatca catttcccaa cggataccag atcctttttg
      421 gggggttgga tgattctgaa agaatcaaat caatcacacc gcaaaaagga gtaattacgg
      481 atatatggtg cgaagaagcg acagaatttg aagaagatga tataaaacag ttaaataaaa
      541 gactcagagg ccgggcggca gcagtaaaac gcctggtttt atcatttaac cctataatca
      601 aaaaccactg gatctttaaa acatacttta attactggaa tggagataat aataggtatc
      661 aaaatgatag aatcagcata ttaaaaacga cctaccgtaa aaataaattt ttaacacctg
      721 atgacataga ccggctgctt tcggaaaatg atccctatta cagagatgta tacatcgaag
      781 ggaactgggg tgtgctcggt gatatcatat ttaaaaactg ggaaataaaa gatatatccg
      841 gcatgaaaaa cagggtggac aggtcttgtc acggtctaga cttcggattc agcaatgatc
      901 cggctgcagt attaaaacaa tactttgaca aatcttctaa taacctctat atattcgacg
      961 aaatatacga aaaagggctg acagataaga aattagcgga cctaacatat aagctgatag
     1021 aatttgaaga agtaatctgt gacagtgcag aacctaaatc gatcaaacat ctaaacaatc
     1081 acaaacttaa tgcaagagga gcaaaaaaag gtccgggcag tattgaaact agatataggt
     1141 ggtattcagg tatcaatata atcttaagcc agaagtgtgt aaacataaaa agagagctgc
     1201 agacccacca gtggaaaaaa gataaagacg gcaattcact taaagtaccg gaagataaaa
     1261 acaaccatgc aatagatgcc agcatgtacg ggctgagtga ttactggaac ttagagatgc
     1321 agagctggta tacacaggat tatgtataag gagatgagtt atggcgctaa caggtagtga
     1381 ggcagtgaag atgtcctttt tagaatggat atacagccag gataacttcg ataggataaa
     1441 actgtatgaa gaaaacgagc ggtattacga cggtgatata gatataaaac tacctgccaa
     1501 cgtaaaaaaa tacttagctc aggaatacgg atatacgggt aatatctgca gggctgtagt
     1561 tgatgccagt gtaggatttt tatcaaaaga accggtatct atcgaaatac aggaagaaga
     1621 gggtaaagaa gaaaatatca aaaaagcaga agaatggata tacgaaaaat ttagaaacaa
     1681 caacttattg ataaaagatt tcatcaaggc actcaggatc caggcaaaaa aaggcgaatt
     1741 tgcctacaag ataagcagaa tactggatga taatgatgaa aaagttgcag attataagat
     1801 aacagttttg aaaccggata tttgttttcc caaatggaaa gacgaatcat atgaagaaat
     1861 ggaatacttt gctgttgaat atgtgagata caacagcagg aaaaaagaaa aagaatggtt
     1921 tgctcaggtt atctggcctg atctagtaaa agaatacagc agaccgttag gaagtagtaa
     1981 agatcagtgg aaattgatta acagttggga aaacaaatat ggtttcataa acgtagaatg
     2041 ggttgaaaac aagatagatg attcggcctg gagcgaatca gatatcacag aagatctaaa
     2101 ggatttacaa aacgcattaa ataaatcaat tacggattta gtatatacat ctgataaaga
     2161 agcatttacg cagaccttcc tcctaggggc aaatcagccg ctggatcccg aaaccgggga
     2221 acctcaggac atagaaactg ggccgggaaa actgcatata ataccggctt cggctgataa
     2281 aatccccaaa ctaggagtaa tagaggcagg taactttcag ggtttgcacg acacattaga
     2341 taagtactta gatttggtaa gtataataac aaaggtcccg cgtatagagc taaatagaat
     2401 ggggagtagt gatctcggga ccggcggtgt cccgacagta attgccttga ggacaatata
     2461 ccagtcattt atagggaaga ctaatgaaaa atcaaactta ataaaatcag gtttggagaa
     2521 cactgcagaa aaattattta agatggctga agtcgatgac atagatacta agtttgaaca
     2581 tcaagagttc aaaactagga ttcatataag atcaggacta ccttctgatg ataaagaaaa
     2641 aatggaagtt caggaaaaag agatcaacaa taaaataaaa tcaagagaaa cagtaat
//
LOCUS       DATGCJ010000146        12553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_109628, whole genome shotgun sequence.
ACCESSION   DATGCJ010000146 DATGCJ010000000
VERSION     DATGCJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109628"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..699)
                     /locus_tag="VKN64_07295"
     CDS             complement(13..699)
                     /locus_tag="VKN64_07295"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMA60082.1"
                     /translation="MKNTPYHIKVKMIVLILFFIGISILAFFLRGDIYAVIKDPVLLK
                     NWMSVFGSFQIIMFILIQAVQVIVFIVPGELVEIAAGFLFGTTLGSIYSIIGIAFGSA
                     ASFIIARVLGYDFVKWVVPEQMFNRWNIFINEDKRGGTVLFLLYFIPGTPKDALAYFA
                     GITPVSYLKFIFISMFARLPAIVFSAYIGANIEQKEYTNALIVSVIAGIAFLIGFIYR
                     DRIIKWLYQL"
     gene            complement(772..1149)
                     /locus_tag="VKN64_07300"
     CDS             complement(772..1149)
                     /locus_tag="VKN64_07300"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HMA60083.1"
                     /translation="MFKKIIIAVLVIIIGMSIYFAVVFSGMDDKINAVQISEINLSEV
                     EDGIYRGEERIGLVGSVVEVEVKDHKITNITLIDHRNWRGKPAEAILGEVIAQQSLMV
                     DTVSGATASSKVILKSIENSLIK"
     gene            complement(1167..1760)
                     /locus_tag="VKN64_07305"
     CDS             complement(1167..1760)
                     /locus_tag="VKN64_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA60084.1"
                     /translation="MVDKNMQEQRMKNYFIEAGYSILLKQGTEQLTVRNIAQKAGYSY
                     ATIYNYFNNFDELMWAIGVKVLDILADTLNEHYEQIKTKLDDRKVLKSVYKKYIDYFL
                     ENENLYHFIFFKNINIEDIEQGNKAQPPVLYKLQQKIMEKCFKKGVIKKADIVIAGDL
                     LTNSINGLLSLYFSGKENISKKELYLKAEKYIDYIIS"
     gene            complement(1762..2238)
                     /locus_tag="VKN64_07310"
     CDS             complement(1762..2238)
                     /locus_tag="VKN64_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMA60085.1"
                     /translation="MKTLIIYASKHGSAEKAANKLYQHLGDEKEIVNIQENSKPFLEN
                     FDTVIIGGSIYGGKIQKEIQKFIEDNLETLLSKRIGLYLCCGIEKDFEKQLENSMPEK
                     LLGHTSVVGYFGYEYNIDKMNFLEKTMVKFVAKDKAGKSNINEENIKEFAREIKGE"
     gene            complement(2434..3423)
                     /locus_tag="VKN64_07315"
     CDS             complement(2434..3423)
                     /locus_tag="VKN64_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMA60086.1"
                     /translation="MMKKVGIIILGLVVIVGMFLFLNLQNAKAQEAKDHPALSKYEGG
                     EIIAYQVEDYAPYVLGTGPQEEKGEEFRGHRKYFSEFIDLEGKVTRIQYVINNEEGLF
                     KVFKNYEQALKEAGYQILFTTSDKESSWPFWNETVYHHEWGINPVRGDQFRDPFGRNG
                     FYFLTAKGTYNGNNIYFALFINPDQDDIIITQDVIEINPMESCLVTAAKIEDDIKSSG
                     FVSIYGIHFDTDKAVIKEESKPALKEIALFLNNHSDKKYYIVGHTDNVGDLSYNMNLS
                     KKRAEAVMNELINGYGVDSTQIEAYGVASLAPVTSNATEAGKARNRRVEIVEQ"
     gene            complement(3789..4442)
                     /gene="hisIE"
                     /locus_tag="VKN64_07320"
     CDS             complement(3789..4442)
                     /gene="hisIE"
                     /locus_tag="VKN64_07320"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HMA60087.1"
                     /translation="MLSNNLRLLEEVDLKYNEKGLIPAVVQDIDTKDVLMVAYMNEES
                     LQKTIESGETVFFSRSRQELWHKGETSGNTQKVEELYYDCDKDTLLILVEPAGPACHT
                     GNTSCFYRKLAESKDKKERYNKDEIINMLYNLIKSRKNELPEDSYTTYLFEEGIDKIL
                     KKIGEESSEVIIAAKNEPQHELVYETADLIYHLMVLLVEKDVNLDQIKDELSDRYKS"
     gene            complement(4432..5226)
                     /gene="hisF"
                     /locus_tag="VKN64_07325"
     CDS             complement(4432..5226)
                     /gene="hisF"
                     /locus_tag="VKN64_07325"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HMA60088.1"
                     /translation="MIKILKKRIIPCLDIKDGRVVKGINFLDLVDAGDPVESASRYNK
                     MGADELVFLDITATKEKRKTLVGLVEKVAKEVFIPLTVGGGIKTISDMQYILRAGADK
                     ISVNSAAVKNPPLVKKGAKIFGSQCIVGAVDAALREDESGWDVYINGGSKKTDRDLVE
                     WVKQLEDLGAGEILLTSMNRDGTKEGFDIEMLKAVTEAVNIPVIASGGAGGIEDFIEV
                     FTKTDVDAALAASIFHFGEVDMGDLKQTLADNGIPVRIEEGSDNVE"
     gene            complement(5219..5938)
                     /gene="hisA"
                     /locus_tag="VKN64_07330"
     CDS             complement(5219..5938)
                     /gene="hisA"
                     /locus_tag="VKN64_07330"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMA60089.1"
                     /translation="MIILPAIDIKDGRVVRLKQGDFDKETVYSDSPVKVASQFAQFGV
                     KWLHIVDLDGASSGASRNLDLIKQITDKTNFKIQCGGGVRKKEDVKKMLEAGIDRVII
                     GTLAVKNPELLIEILDEFDHDKILISIDARKGKVATSGWKEESDIEVLEFAQMLEEKG
                     VKYILYTDIARDGMLVGPDFEGLIKLKENTDLNIIASGGISALDEVERLNELDFYGVI
                     IGQALYTGKFSLKEALSRVGD"
     gene            complement(5939..6538)
                     /gene="hisH"
                     /locus_tag="VKN64_07335"
     CDS             complement(5939..6538)
                     /gene="hisH"
                     /locus_tag="VKN64_07335"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMA60090.1"
                     /translation="MVGIIDYGAGNLNSICKAFEHIGFTTEIIEEKIDTDKYEAVVLP
                     GVGAFPGAMDRLRANNLVEFIKEFVKTNKPMLGVCLGMQLLFEWGYEDTKTKGLDLLP
                     GEVVKMESSLKIPHMGWNELEIIKEDPLFKGLPDNPHYYFVHSYKLKEITEEVIAVTE
                     YGGKVPAVVKKDNIIGLQFHPEKSGNNGLKLLENFGELI"
     gene            complement(6539..7126)
                     /gene="hisB"
                     /locus_tag="VKN64_07340"
     CDS             complement(6539..7126)
                     /gene="hisB"
                     /locus_tag="VKN64_07340"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HMA60091.1"
                     /translation="MSKERKAVKKRATYETEVEVELNLDGEGKGDIDSGLKFFDHMLA
                     QLAKHGGFDLMVKAEGDLEIDEHHTVEDVGITLGQAFNQALGDKKGIARFADSHTPLD
                     ETLVRTAVDISGRSYLYADFDFSREMVGDFPSELVWEFLKSFVNHAKITLHMDLIRGD
                     NCHHQIEAAFKSLAVTLKKAVEVVSDRMPSTKGKL"
     gene            complement(7110..8201)
                     /gene="hisC"
                     /locus_tag="VKN64_07345"
     CDS             complement(7110..8201)
                     /gene="hisC"
                     /locus_tag="VKN64_07345"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMA60092.1"
                     /translation="MEIDIKKLVRPEVAKIDTYKGEKDTGSYRLDLSGNESPYDLPEH
                     IKEEITDKIMQNPLNYYPTLYTESLRCSIAEYLDINVDCDQVLVGNGSDDVLDMLIDT
                     FIEPGDRVLAAAPTFSMYRFFSELAGGIYENFSLADREFTYHNIKEMIDRLKPKIIFL
                     CSPNNPTGELLSQETILKLAEVYEGVLVIDEAYAEFAKSSLLTHVNEYTNLAVTRTFS
                     KAFGLAGIRLGYLVGSRALIRQVGKVLKPYNINSITSLIGKIILREGDLIEKRVDKII
                     KEREKMFALFKEYDNWKVFPSSANFVYIEGAATMQFKRMLNRVGIKIRSFDRQPPAIR
                     ITVGKPAENDEVAEIFKQFKEGKGDEQRT"
     gene            complement(8191..9510)
                     /gene="hisD"
                     /locus_tag="VKN64_07350"
     CDS             complement(8191..9510)
                     /gene="hisD"
                     /locus_tag="VKN64_07350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMA60093.1"
                     /translation="MLKKIDLRKRSVDEIKDILKERSQKDLSEYRKVARDIIEDIKKR
                     KDKALFAYTARFDKIELDASTVKVTQAEIESAVQEVDNKFLNALKKAAQNIEEFHEHQ
                     KESSWQKQTSPGKITGQICRPLAKAGIYVPGGRAAYPSSVLMTALPAKVAGVEEVVMV
                     TPPGKEGKLNPKILAAAQVAGVDSIYKVGGAQAVAALAYGTESIPKVDIVVGPGNIYV
                     TMAKELVFGQVKIDMTAGPSEVLVLADGQAEPAYIAADLMSQAEHDPLASSILVTNSA
                     DLGPKVEKEIKKQIEDLEKKDIIKESLSNYGLLIEVEDMESGIEAINQIAPEHLELAV
                     EKPFDLLPEIKNAGSIFLGEYTPEPIGDYYAGPNHVLPTEGTARFFSPLAVRDFVKYS
                     SFIYYSREALKEASDDVITLAEGEGLTAHANSIKIRKESIEDDVNGN"
     gene            complement(9517..10146)
                     /gene="hisG"
                     /locus_tag="VKN64_07355"
     CDS             complement(9517..10146)
                     /gene="hisG"
                     /locus_tag="VKN64_07355"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMA60094.1"
                     /translation="MSNIRIAMAKGRLAKKAVKLFRAADINLPEDMLSSRKLIFPLPE
                     ENLEIVLVKPADVPIYVEYGTADLGIVGKDTLLEENRDLYEVLDLQFGKCRFALAGLP
                     EKKESLLERKVATKYPDFTRRYFRSKGEPVEIIKLNGSIELAPLTGLADTILDIVQTG
                     RTLKENGLVVYENIRQLSARLVVNKVSMKTSQKAITKIIDALEEEVEGG"
     gene            complement(10146..11420)
                     /gene="hisZ"
                     /locus_tag="VKN64_07360"
     CDS             complement(10146..11420)
                     /gene="hisZ"
                     /locus_tag="VKN64_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HMA60095.1"
                     /translation="MNAFVEGVRDILPAELRRRRAIYKRIRKVFQNADYREVITPTLE
                     SLELYSGIEGVIDKSEMFKVVDENGQILVLRPDVTMPIARLAATRFQDTPRPLKFSYL
                     TTAYQSKNSQSLSLKEKTQAGVELIGSDKLCSDIEMIALLIKTMKAIHIEKPLIDIGH
                     ADLINEIFCTMDLTEKQRDNLLSLLSAKNKVEIRRYLNEIEIAEESKEVLMKLPTLFG
                     KPDDVIPELKQLPISEEAHQEVLKLEKLFNRLDELGLIENITFDPMLISRHGYYTGVI
                     FKGYAGSHNVLLANGGRYDNLTEKFGVGEPAVGFALEIENVLEYLNKTHYEFEAQSAR
                     VIINYHEEDNFSDAYHLAEELRDKDYIVEIFEEGKDQEYIDFQGLDFQIECIEDNLVI
                     SNIKNGKELTVNKKDSDNNLAEVVIDKLKEVK"
     gene            11668..12474
                     /locus_tag="VKN64_07365"
     CDS             11668..12474
                     /locus_tag="VKN64_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HMA60096.1"
                     /translation="MLFDYHMHTNFSADSKYTMEEMCEAAIEKKLDEIAITDHHDIDY
                     QDDSMEFLLDKENYLKEIEKYQEKYEGKLTIKKGIEMGLQTHILKESDDFLDNDFDFI
                     ICSFHTAEKKDLYNGDFFQNYSQWEAYLRYLESILEVVQNFENYSVLGHLDLVRRYDY
                     YATKPDLMDNEKAEEIIREILKEVIRRNKGIEVNTTGYYIEEGNNPNPSVKVLKLYKE
                     LGGEILTIGSDSHSPDQIAYKFPETLTLLKEIGFDKLTTFSNMEPEFHKI"
BASE COUNT         3770 a         2597 c         1825 g         4361 t
ORIGIN      
        1 aataattatt agtcataatt ggtaaagcca cttaattatt ctatccctgt aaataaatcc
       61 tattaaaaaa gcaatccctg ctatgacaga aactatcagg gcatttgtat attctttttg
      121 ttcaatattt gctccgatat aagcggaaaa aacaatggcg ggtaaacggg cgaacatact
      181 gataaaaatg aacttaagat aacttacagg cgtgattccc gcaaaatagg cgagggcatc
      241 cttcggtgta ccggggataa aatagagtaa aaagagaact gttccccctc ttttatcttc
      301 atttataaat atgttccatc tattaaacat ctgttctggg acaacccatt ttacaaaatc
      361 atatcctaaa actctagcta ttataaaact ggccgctgaa ccaaaggcaa taccaataat
      421 ggaatagatt gatcctaacg tagttccaaa taaaaagccc gctgctatct caactaattc
      481 gcctggtaca ataaagacaa tcacctgtac ggcctgaatt aaaataaaca ttataatttg
      541 aaaagatcca aatacagaca tccaattttt taatagtaca ggatccttta taacagcata
      601 aatatctccg cgcaaaaaga aggcaagtat tgaaatacca ataaaaaaca atattaaaac
      661 tatcattttt accttaatat gataaggagt attcttcata aaaaatccct catttaaaat
      721 ctttctcccc ggttagttta aatcaaaaat taaaaagatt aaataaaaac gctatttaat
      781 taggctattt tcaattgact ttaatattac tttactacta gccgttgccc ctgatactgt
      841 atcaaccatt aaactctgtt gggcgatgac ttcacctaaa attgcttccg caggtttgcc
      901 gcgccaattt ctatggtcta tcaaagttat attggttatt ttatgatctt ttacttccac
      961 ctcaacaact gaaccaacta aaccgatcct ttcttcccct ctatatattc catcttcaac
     1021 ttcacttaga ttaatttcag aaatttgaac cgcattaatt ttatcatcca taccggaaaa
     1081 aacaactgca aagtaaatac tcatccctat aattatgacc aaaaccgcaa ttattatttt
     1141 tttaaacata tgcctctttc ctttccttaa gaaataatat aatctatata cttttcagct
     1201 tttaagtaaa gttccttttt actgatattc tctttaccgg aaaagtataa agacaacaaa
     1261 ccgttaatag aattagttaa aagatctcct gctatcacta tatctgcttt tttgattacc
     1321 ccttttttaa aacatttctc cattattttt tgctgtaatt tatatagaac aggaggttga
     1381 gccttatttc cttgttctat gtcctctata tttatatttt taaagaatat gaaatggtat
     1441 aaattttcat tttctaaaaa gtagtctata tattttttat aaactgactt gagtactttt
     1501 ctatcatcta attttgtctt tatctgttca taatgttcat ttaaggtgtc ggctaaaata
     1561 tctaatacct ttacaccaat tgcccacatc agttcatcga agttattgaa gtaattatat
     1621 atcgttgcat aagaataacc agctttttgg gctatattcc ggacggtcag ttgttcagta
     1681 ccctgtttta ataagatgct gtaaccggct tctatgaaat aatttttcat tctttgttcc
     1741 tgcatgtttt tatcaaccat attattcacc ctttatttct ctagcaaatt ccttaatatt
     1801 ttcttcatta atattagatt taccagcttt atctttggct acaaacttga ccattgtttt
     1861 ttctaaaaaa ttcatcttat caatattata ctcatatcca aagtatccaa ccactgaagt
     1921 atggccaagt aatttttctg gcatagaatt ctctaattgt ttttcaaaat ctttttctat
     1981 cccacagcag agatacaaac caatcctttt actcaaaagt gtttctaaat tatcttcaat
     2041 aaatttttga atctcttttt gtattttacc tccataaatt gaaccaccaa taatgaccgt
     2101 atcaaagttt tctaaaaaag gtttgctatt ttcttggata tttactattt ctttttcatc
     2161 tcctaagtgt tgatataact tgttggccgc cttttcagca ctaccgtgtt tgctcgcata
     2221 aatgatgagg gttttcacta atactcactc cttggttaac aatgttaata tatttaacaa
     2281 tgttaattat atacctttat ctactctatg tcaatacaaa aaaataggaa aagatatata
     2341 ttttaataga tgataaatgt actaagggga gtttgtggca ttaaatacca tcaactcccc
     2401 tcatgacttt actatgttat tttatggttt aaatcattgt tcgactattt ctactctgcg
     2461 attccttgct ttgcctgcct cagtagcatt cgaagtaacc ggggccaggg aagcaactcc
     2521 atatgcttcg atttgtgttg aatctactcc ataaccgttg atcaattcat tcataactgc
     2581 ttcggctctt tttttagata aattcatatt ataagataga tctcctacat tatcagtgtg
     2641 acctactata taatactttt tatcagaatg attgttcaaa aataaagcaa tttcttttaa
     2701 agcgggttta gattcttctt taataacagc cttatctgtg tcaaaatgaa taccgtaaat
     2761 tgaaacaaac ccactcgatt ttatatcatc ttcaattttt gcagcagtta ctaaacaact
     2821 ttccatggga ttaatttcaa ttacatcttg agttataatt atgtcatctt gatcagggtt
     2881 gataaaaaga gcaaaatata tattgttccc attataggtt ccttttgcag ttaaaaaata
     2941 aaatccgttt ctaccaaaag gatcgcggaa ttgatctccc cgaacgggat taatacccca
     3001 ttcatgatgg taaacagttt cattccaaaa tggccaactg ctttccttat cggaagtagt
     3061 aaatagaatt tgatagccgg cttctttaag ggcttgttca taattcttaa atactttaaa
     3121 taaaccttcc tcattattaa taacatattg aatccttgtt actttacctt ctaaatcaat
     3181 gaattctgaa aaatatttac gatgccctct gaactcttcg cctttttcct cttggggacc
     3241 agtacctaaa acataggggg cataatcctc tacctgatat gctataatct cgcccccttc
     3301 atattttgat aaggcgggat ggtctttagc ttcttgtgct tttgcatttt gtaaattcaa
     3361 aaataaaaac atccctacaa taacgaccaa tcctaaaatt attatcccta cttttttcat
     3421 catgaaaatc tcccttctct atttaaaatt aaaaaaacat aatataatag atacttcaat
     3481 atttattact ttatctattt ataattcttg ttacatgtat aattatatac ttatatgcaa
     3541 ctgtattagt tccttcaaat caacttaatt cttttaaatt atccggtaaa atatctgaca
     3601 caaaaagaaa cctagtcatt attatcaatt aatatttttt ccatcatagg gtgaccaatt
     3661 tttttataat attaatcact ttctgcaaaa attatttaca aacctgtaaa actatatttc
     3721 ccaaaagaaa taaaaaagct tgcccatata aggcaagctc aaggttaata tatctacttt
     3781 ttttatctct atgatttata tctatcactt aattcgtctt tgatttgatc taaattcaca
     3841 tctttttcga ccagtaaaac catcaggtga taaattaggt cagctgtttc atagacaagt
     3901 tcatgttgag gctcattttt agcagcaata ataacttctg aagattcttc gccaatcttt
     3961 ttaagaatct tatcgatacc ttcttcgaaa agataagttg tataggaatc ttcgggtagt
     4021 tcattctttc tgcttttaat taagttatat aacatattta taatttcatc tttattatat
     4081 ctttcttttt tatccttact ttccgctaac tttctgtaaa aacaagaagt gttgcctgta
     4141 tgacaggccg ggccggccgg ttccaccaaa attaataggg tatctttatc acagtcataa
     4201 tagagctctt ctactttttg ggtattaccg gatgtttctc ctttatgcca taactcttgt
     4261 ctacttctgc tgaaaaagac agtttctcca ctttcaatgg tcttttgtaa agactcttca
     4321 ttcatatatg ctaccattaa gacatctttg gtatctatat cctgcactac agccggaatt
     4381 aagccttttt cattatattt taaatcaact tcttctaaca atcttaaatt attactcaac
     4441 attatcacta ccttcttcaa ttctaaccgg gattccatta tcagccagag tttgctttag
     4501 atctcccata tcaacttctc caaagtgaaa tatggaagct gctaaggctg catcaacatc
     4561 agtttttgtg aatacttcaa tgaaatcttc tatcccaccg gctcctcccg aggcaattac
     4621 gggtatatta accgcctctg ttactgcttt taacatctca atatcaaagc cctctttagt
     4681 accatctcta ttcatactgg ttaaaaggat ctccccagca cctaaatctt cgagttgttt
     4741 tacccattca actagatccc ggtcggtctt tttactgcca ccgttgatat agacatccca
     4801 gccgctttca tcttctctta aggctgcatc aactgcgcct actatacatt gggaaccaaa
     4861 tatcttagct ccttttttaa ctaaaggagg atttttaaca gcagccgagt taacagagat
     4921 cttatctgcc ccggctctta agatatactg catatcagat atagtcttaa tccctccccc
     4981 aactgtgaga ggaataaaaa cctctttggc aactttttcc actaagccaa ccagggtctt
     5041 ccttttttct ttagtagcag taatatctaa aaagaccagc tcatctgctc ccatcttgtt
     5101 atagcggctg gccgattcta ccggatcccc ggcatcaacc aaatctaaaa aattaattcc
     5161 ttttacaaca cgtccgtctt ttatatccag acatggtatt atcctctttt tgagaatctt
     5221 aatcacccac cctacttaac gcttctttta aggaaaattt accggtgtat aaggcctgac
     5281 caattattac accgtaaaaa tctaattcat ttaacctttc aacttcatca agggcactta
     5341 taccacccga agcaataata tttaaatcgg tattttcttt taatttaata agcccttcaa
     5401 aatcaggtcc tactaacata ccgtctcttg ctatatcagt atagagaata tacttcacac
     5461 ccttttcttc taacatctgg gcaaattcta atacttcaat atcactttct tctttccagc
     5521 ctgaggtggc aactttgcct tttctagcat ctatactaat taagatctta tcatgatcaa
     5581 actcatctaa gatttcaatc agcaattctg gattttttac tgctaaagtg ccaataatta
     5641 ctctatcaat accggcctca agcatctttt tgacatcttc tttctttctt acacctccac
     5701 cacattgaat tttaaagttc gttttatcgg taatttgttt tattaagtct aaattacggc
     5761 ttgcaccaga agaagctcca tctaaatcta cgatatggag ccatttaaca ccaaactgag
     5821 caaactgact tgcaacttta accgggctat cactatacac tgtttcttta tcaaaatctc
     5881 cctgttttaa cctaacaact ctcccgtctt taatatcaat tgcaggtaat atgatcaatc
     5941 aaatcagctc cccaaaattt tctaataatt ttagaccatt gtttccactt ttttcagggt
     6001 gaaactgtaa gccaataata ttatcttttt taaccacggc cgggacctta cccccatact
     6061 cggtgacagc aataacttct tcggttattt cttttaattt ataagaatgt acaaaataat
     6121 aatggggatt gtctggtaaa cctttaaaca aaggatcttc cttaattatc tctaattcat
     6181 tccagcccat atgcggtatc tttaaactgc tttccatctt gacaacttca cccggcaaaa
     6241 gatctaagcc ctttgtcttg gtatcctcat aaccccattc gaataaaagc tgcatgccga
     6301 ggcaaactcc caacatcggt ttgttagtct taacaaactc ttttatgaat tcaaccaagt
     6361 tattggctct taacctatcc atggccccgg gaaatgctcc tacccccggt aagacaactg
     6421 cttcatattt atctgtatct atcttttctt cgattatctc ggttgtaaaa ccgatatgtt
     6481 cgaaagcctt acagatacta tttaagttac ctgccccata atcaattatg ccaaccatct
     6541 ataatttccc cttggtagag ggcattctat cagaaaccac ttctacagcc tttttgaggg
     6601 taacagctaa agacttaaag gccgcttcta tttgatggtg acagttgtct cctctaatta
     6661 aatccatgtg taaggttatc ttggcatggt tgacaaaaga ctttaaaaat tcccagacca
     6721 gttcggatgg gaagtcgccc accatttctc ggctgaaatc aaagtccgca tatagataag
     6781 atcggccgct gatatcaaca gctgttctga ccagagtctc atcaagaggg gtatgactgt
     6841 cagcaaaacg ggcaatgcct tttttatctc ccaaagcttg attgaaagcc tgtcctaagg
     6901 ttatacccac atcttccacg gtgtggtgct catcgatctc taagtctccc tccgccttaa
     6961 ccattaaatc gaacccccca tgtttggcca gctgggctaa catgtggtcg aaaaatttaa
     7021 ggccggagtc aatatctccc ttaccttctc catctaaatt aagctcgact tcaacttcag
     7081 tttcataagt agctcttttt ttaacagctt tacgttcttt gctcatcgcc ttttccctcc
     7141 ttaaactgtt taaatatttc agcaacctca tcattttcag ccggttttcc caccgtaatt
     7201 ctaatggccg gtggttgtcg atcaaaagac ctaatcttta ttcccactct atttaacatt
     7261 cttttaaatt gcatggtagc ggctccctct atatatacaa agttagccga ggaagggaat
     7321 accttccagt tatcatattc tttaaaaagg gcgaacatct tttctctttc tttgattatc
     7381 ttatcaaccc ttttttcaat taaatcccct tctcttagga ttatcttacc gataagggaa
     7441 gttattgaat taatattgta tggttttaaa actttaccga cctgacgaat taaggctcgg
     7501 ctgccgacca ggtaaccgag tctaatcccg gccagtccaa aagctttgga aaatgttctg
     7561 gttacggcta gattagtata ttcatttaca tgggttaata aggaactttt agcaaactct
     7621 gcataggctt catcgataac taatactcct tcatatactt ccgctaattt taatattgtc
     7681 tcctgagata atagttctcc ggtcggatta ttaggagaac aaagaaaaat tatctttggt
     7741 tttaacctat ctatcatttc tttaatatta tggtaagtga attcccgatc tgccaaggaa
     7801 aagttttcat agataccacc cgccagttcg gaaaaaaatc tatacattga aaaagttggg
     7861 gcagccgcta atactctatc acctggttca ataaaggtat cgattaacat atcaagcaca
     7921 tcatcggaac cattccctac taacacctgg tcacagtcta catttatatc taagtattca
     7981 gctatactgc accttaatga ttcagtatat aaagtggggt aatagttcaa aggattttgc
     8041 attatcttat ctgtaatttc ttctttgatg tgttcgggta aatcataggg tgattcatta
     8101 ccggaaagat ccaggcggta actgcctgta tctttttctc ctttatatgt atctattttc
     8161 gctacttctg gtcttaccaa ttttttaata tcaatttcca tttacatcat cctcaatact
     8221 ctcttttctt atttttatag agttggcatg ggcggttaaa ccttcaccct ctgccagggt
     8281 aataacatcg tctgaggcct cttttaaagc ctctcggctg taatatataa agctggagta
     8341 cttaacaaag tctcttacag ccagcggtga gaaaaagcgg gctgtacctt cggttggtaa
     8401 tacatgattt ggccccgcgt aatagtcccc aatcggttcg ggagtatact ctcctaaaaa
     8461 gatcgaacca gcatttttaa tttctggtaa gagatcaaaa ggtttctcta cagctaactc
     8521 caaatgttcg ggggcaatct gattgattgc ctcaatacca ctttccatat cttctacttc
     8581 aattaaaaga ccgtaattag atagagattc cttaattata tcttttttct ccaggtcttc
     8641 aatctgtttt tttatttctt tttcaacctt tggccctaaa tcagctgaat tagtaactaa
     8701 aattgaagaa gccagcggat cgtgttcggc ctggctcatt aaatcagctg ctatataggc
     8761 cggctcggcc tgtccatctg ctaatactaa tacttcactt ggcccggctg tcatatctat
     8821 ttttacctga ccaaatacta attctttggc catcgtcaca taaatattac cagggcccac
     8881 tacaatatct actttaggga tactttctgt accatatgct aaggctgcta cggcctgggc
     8941 tccccctact ttatagatgc tgtccacacc tgctacttga gctgcagcca gtattttggg
     9001 atttaattta ccttccttac cagggggagt aaccataact acctcttcta cacctgccac
     9061 tttggcaggg agcgctgtca ttaaaacaga ggaaggatag gctgcccgac cgcccggtac
     9121 ataaatacca gctttagcta aggggcgaca gatctgcccg gtaatcttgc ccggagaagt
     9181 ctgtttctgc caggaacttt ctttttgatg ttcatgaaat tcttcaatat tctgcgcagc
     9241 cttttttaag gcatttagaa atttattatc aacttcttga accgcgcttt ctatttcagc
     9301 ctgagttact tttaccgttg aagcatctag ctcaatctta tcaaaacggg ctgtgtatgc
     9361 aaaaagggct ttatcctttc ttttttttat atcttcaata atatcgcggg ctactttcct
     9421 atattcagat aggtcttttt gggaacgttc ttttaatata tcctttattt catctacact
     9481 tctttttctt aaatctattt ttttaagcat ctgtctttaa cctccctcaa cctcttcttc
     9541 tagagcatct attattttag taatagcttt ttgactagtt ttcatactta ccttatttac
     9601 aaccaacctt gcactgagct gtctaatatt ttcatatacc accaagccgt tttcttttaa
     9661 agtacggccg gtctgtacta tatctaaaat tgtatcggct aaaccggtta aaggagctaa
     9721 ttcgattgac ccatttaatt taattatctc aaccggttct cccttacttc taaagtatct
     9781 cctcgtaaag tcaggatact tggtagctac ctttctttcc agtaatgatt cctttttttc
     9841 cggtagaccg gctaaagcaa acctgcattt accaaactgt aagtccaata cttcatatag
     9901 gtctctattt tcttctaaaa gggtatcttt tcctactatc ccgaggtcag ctgtcccgta
     9961 ttctacatag atgggtacat cagctggttt caccagaaca atttctagat tttcttccgg
    10021 gagagggaaa attaattttc tggaacttaa catgtcttca ggaagattga tatcagccgc
    10081 cctaaaaagc ttaactgcct ttttggctag ccttccctta gccatggcta tacggatatt
    10141 actcatcatt ttacctcctt gagcttatct ataacaactt cagctaaatt attgtcgcta
    10201 tcttttttat taacagtaag ttcttttccg ttttttatat tacttatcac taaattgtct
    10261 tctatacatt caatctgaaa gtcaaggccc tggaagtcaa tatattcctg gtctttgcct
    10321 tcttcaaata tctctacaat atagtccttg tctcttaact cttccgctag atggtaggca
    10381 tcagagaaat tatcttcctc gtggtaattg ataattactc tagcactttg agcttcaaat
    10441 tcataatggg ttttatttaa atactccaat acattttcta tttcaagagc aaatcctaca
    10501 gctggttccc ctaccccaaa cttttctgtg agattgtcat aacggccgcc gttggctaaa
    10561 agaacattat ggctgccggc atagccttta aagattacac cggtgtaata accgtggcgg
    10621 gagatcaaca tcggatcaaa ggtaatattt tctattaagc ctaactcatc taacctatta
    10681 aagagctttt ctaactttaa tacttcctga tgagcttctt ctgagatagg aagttgtttt
    10741 agttcaggta tcacatcatc tggtttgcca aacaaggtag gcagtttcat taatacttct
    10801 ttactttcct cagctatttc tatctcattt aaatatcttc ttatttcaac tttgttttta
    10861 gctgaaagca atgacaaaag attatctctt tgtttttcag ttagatccat tgtacaaaag
    10921 atttcattta ttaaatcagc atggccgata tcaattaaag gcttctcaat atgaattgcc
    10981 ttcatcgtct taattaaaag ggcaatcatt tcaatatcag aacaaagctt atcactgccg
    11041 atcagttcaa cacccgcctg ggtcttttcc tttaaactta aactctgtga gtttttagac
    11101 tgataggcgg tagttaagta agaaaactta aggggccgcg gtgtatcctg aaagcgggtg
    11161 gccgctaaac gagcgatcgg catcgttaca tccggcctca aaaccagaat ctggccgttt
    11221 tcatcgacta ctttaaacat ttcgctctta tctatgacac cctcaatccc tgagtaaagt
    11281 tcaagtgatt ctaaagtagg agtgatgacc tccctatagt cagcgttttg aaataccttt
    11341 ctgatccttt tatagatagc tcttcttctt ctcaattcag ccggaagtat atcccggact
    11401 ccttctacaa atgcattcat cttattaaag cctcctaaac tttttataaa gtgatttatc
    11461 tattttaaaa tatgaatagg attaagtctg attttaatta taatattata caacccaata
    11521 ttaaatcaga cttcatattt taaggtcttt gatgttaatt atacaataat tttattagag
    11581 atgcattaat atttcaaata aagttggatt aatgttataa taatagagta tatttagaga
    11641 aaaatatagg ataggagtga ttattacatg ctatttgact accacatgca taccaacttt
    11701 tcagctgact ccaagtatac aatggaagag atgtgtgagg cagcgattga aaaaaaatta
    11761 gatgaaattg ccattacaga ccatcatgat atagattatc aggatgactc catggaattt
    11821 ttactggata aagaaaatta tttaaaagaa attgagaaat atcaagagaa atatgaagga
    11881 aaacttacta taaaaaaggg aattgagatg ggactgcaga cccatatatt aaaagaaagt
    11941 gatgacttct tagataatga cttcgacttt atcatctgtt cttttcacac tgccgagaaa
    12001 aaagatctat ataatggtga cttttttcaa aactacagtc agtgggaagc ctatttaaga
    12061 tatttagagt caatcttaga agtagtgcag aactttgaaa attacagtgt gttaggacat
    12121 cttgatctgg ttagaaggta tgattactat gcaactaaac cggatttaat ggataatgaa
    12181 aaagcggaag aaattatcag agagatctta aaagaagtga taagaagaaa taaggggatt
    12241 gaagtcaata cgaccggtta ttacattgag gaaggcaata atcctaaccc ttctgttaaa
    12301 gtccttaagt tatataagga actaggggga gagatcttaa cgataggttc ggacagtcac
    12361 tctcctgacc agattgctta taagtttccg gaaactttaa ctttacttaa agagattggt
    12421 tttgataaat taactacttt ttccaatatg gaacctgagt ttcataagat ataatatgta
    12481 tttatcatta aaattattag gtgattacaa agtcctgcta aaattagcag gacttttgca
    12541 tttgtattta taa
//
LOCUS       DATGCJ010000147         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_18852, whole genome shotgun sequence.
ACCESSION   DATGCJ010000147 DATGCJ010000000
VERSION     DATGCJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18852"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VKN64_07370"
     CDS             <1..409
                     /locus_tag="VKN64_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMA60097.1"
                     /translation="DYAHHPTEIINTIKTAQGTKAKKLYVVFQPHRYSRTQNLMEEFS
                     KSFDEVDELILTDIYSASEKPIEGVSSKKLFELIKEHKKYNNLDFGVKYIKDFKEIED
                     YLRHNLKPTDLLVTIGAGNVYEIGENLVGESHE"
     gene            402..1106
                     /locus_tag="VKN64_07375"
     CDS             402..1106
                     /locus_tag="VKN64_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HMA60098.1"
                     /translation="MNKLSIFIILAVLIFTSLFAYAYYSPELNIKTIKLRGVNTFDEN
                     LIISFAEEYIGQNIVYLKKDRIERKLTDVKYIKNAQFKRVLPDTALFTIEESVPFFRY
                     IKDNQYFYIDKQGFIYSQAELQRKIILPVLTGFKIEEGTKRINFDEEMLVFIRNLEEY
                     ISKEAAVPKFIEFKKRKITLKYEGKYDIILGELENISEKFRVLKNVTDQIEQKDYQVK
                     YVDISIYSKPVLKLKQ"
     gene            1218..2504
                     /gene="ftsA"
                     /locus_tag="VKN64_07380"
     CDS             1218..2504
                     /gene="ftsA"
                     /locus_tag="VKN64_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMA60099.1"
                     /translation="MSSRKIVTGLDIGTTKICTIIAEVTSDEEFEIIGIGFSPSKGLK
                     KGVVVDIDKASNAIRESVNKAQIMAGVEVDTAFIGIAGSHISSINNHGVVAVSGDEKE
                     ITKNDIDRVMEAAQIISLPAEEEIIHVIPREFIVDGHKGIKDPRGMSGVRLEVETHIV
                     KGSSTSIQNLVKSVIRAGIEVDDIVLEPLASSKSVLTEDEKELGVILVDIGGGTTDIV
                     VFHEGSIVATYVLPVGGNHVTHDIAIGLRTPISEAEKIKVNYGTTLLEESDEEEDYYI
                     EVLSASGENKREIPRRLLTNVIEPRTNEMYEMINRQIYSAGPKDLTPAGVVLTGGASL
                     LDGSADLAEDVFDLPVRLGKPDYVYGLSDVIDDPLYKNKDGKIPKAIFSTVIGLIEYG
                     LENGPTEVNANSQKTKEKEITESFFSKIKSWFQDFF"
BASE COUNT          961 a          341 c          507 g          769 t
ORIGIN      
        1 tgattatgcc catcatccga ctgaaattat caatacaata aaaacagccc aaggtacaaa
       61 ggctaaaaaa ttgtatgttg tatttcaacc tcatcgctac agccggaccc aaaaccttat
      121 ggaagaattt agtaaaagct ttgatgaggt agatgaactt attttaacag atatatactc
      181 ggccagtgaa aagcccattg aaggtgtcag ttcaaaaaaa ttatttgaac taataaaaga
      241 gcataagaaa tataataatt tagactttgg agttaagtat ataaaggact ttaaagaaat
      301 tgaagattac ttaaggcata atttaaaacc aacagattta ttggttacca ttggagccgg
      361 caatgtttat gaaataggcg aaaatctggt aggtgaaagc catgaataaa ctaagtattt
      421 tcataatttt ggcggtatta atctttactt ccttgtttgc ctatgcatat tattcacctg
      481 aacttaacat aaagacaatt aagttaaggg gtgtaaatac attcgatgaa aatttaataa
      541 tatcttttgc cgaggagtat ataggacaaa atatagtata tctaaaaaag gatagaatcg
      601 aacgcaaact gactgatgtt aagtatatta aaaatgcaca atttaaaagg gtattaccag
      661 ataccgcctt atttaccatt gaagaaagtg tgcccttttt taggtatatt aaagacaacc
      721 agtattttta catagataaa cagggcttta tttattctca agcagaactg cagagaaaga
      781 taattttacc tgttttaacc ggttttaaga tcgaagaagg aacaaaaaga ataaattttg
      841 atgaagaaat gctggttttt atcagaaatc tagaagaata tataagtaaa gaggcagccg
      901 ttcccaagtt tattgaattt aaaaaaagaa aaattacttt aaaatatgaa ggtaagtatg
      961 atattatact aggagaatta gaaaatatat cagaaaaatt tagagtttta aaaaatgtta
     1021 ccgaccaaat tgagcaaaaa gactatcaag ttaagtatgt agatatttca atttatagta
     1081 aacctgtttt gaaattaaaa caataaatta ttataagagg aaaatcatct tttatgttga
     1141 atatttgtaa taatgtgcct ataatatttc acttaaaaat atattaattt ttaaaagaga
     1201 gaggaggcaa aatatttgtg agcagtcgta aaattgtaac aggcctggat ataggaacca
     1261 ctaaaatctg cactatcata gctgaagtta catcagatga agaatttgaa ataattggaa
     1321 ttggattttc accttcaaag ggattaaaaa aaggtgttgt agtagatata gataaagcca
     1381 gtaatgcaat acgggagtcg gttaataagg ctcaaataat ggcaggtgtt gaagtggata
     1441 ccgcctttat aggtattgcc ggttctcata tttcttccat aaataatcat ggagtggtag
     1501 ccgtttcggg agatgaaaag gaaattacta aaaatgatat cgatcgtgtc atggaagcag
     1561 ctcaaatcat ctctttaccg gctgaggaag agattattca tgtgataccc cgagaattta
     1621 tagtagatgg tcataaaggt attaaagacc ctcgcgggat gtcaggtgtt cgtttagaag
     1681 tagaaactca tattgtaaag ggttcttcta cctctataca gaacctggtt aaaagcgtaa
     1741 ttagagccgg aattgaagtt gatgatattg ttctagaacc attggcatcc agtaaatctg
     1801 ttctgacgga agatgaaaag gaattgggag ttattctagt tgatatcggg ggcggcacta
     1861 ctgatatagt agtatttcac gaaggtagta ttgtggctac atatgtgcta ccggtaggag
     1921 gtaatcatgt cacccatgat atagcaattg gacttagaac tcctatctct gaagcggaaa
     1981 agataaaggt taattatggt acaaccttac tggaagaaag tgatgaagag gaagattact
     2041 atatagaagt tctatctgct agcggtgaaa ataaaagaga gattcccaga agactattaa
     2101 cgaatgttat tgaacccagg acaaatgaaa tgtatgaaat gataaataga caaatttatt
     2161 cagctggtcc caaggacttg actccagccg gagtagttct tacaggagga gcttctcttt
     2221 tagatggttc tgcagatctg gctgaagacg tatttgattt accggttaga ctgggtaaac
     2281 cggattatgt atatggttta tccgatgtta ttgatgatcc attatacaag aataaagacg
     2341 gtaaaatacc aaaagcaatt ttctctacag taatcggatt aatagaatat ggtttagaaa
     2401 atggacctac tgaagttaat gcaaattcac agaagacaaa agaaaaagaa ataacagaat
     2461 ctttcttttc aaaaattaaa agttggttcc aagatttctt ttaaaaggag gtatataact
     2521 tgtttgactt aggaacagaa atggaacagt ttgctaatat aaaagttatc ggagttgg
//
LOCUS       DATGCJ010000148        12595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_286625, whole genome shotgun sequence.
ACCESSION   DATGCJ010000148 DATGCJ010000000
VERSION     DATGCJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286625"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1205
                     /locus_tag="VKN64_07385"
     CDS             291..1205
                     /locus_tag="VKN64_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMA60100.1"
                     /translation="MIPDYQKIMLPLLKLAGDEKTHKLRNAIEELADNFDLEEEERKK
                     LLPSGNKNIFDNRVGWAKTYLKKAGLLEYPKRGYFKITKRGKKVLQNNPDKIDNNFLK
                     QFGEFQKFKNTTKSKKKKNKKGKSSEIFDKYTPEEVIELGYTTINNNLASNLLEKIQE
                     NPPAFFEKLVVDLLVNMGYGGTIKDAGRAIGKHGDEGIDGVIKEDRLGLDIIYIQAKR
                     WQNTVSRPEIQKFAGALQGKNAKKGIFITTSKFSKNAIKYGKKINSKIILINGEDLTK
                     LMIKFDVGVSQSSSYKIKKVDNDYFLDE"
     gene            complement(1736..2155)
                     /locus_tag="VKN64_07390"
     CDS             complement(1736..2155)
                     /locus_tag="VKN64_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMA60101.1"
                     /translation="MDIVWEDIIKLLVTAVIATLIGIDREKRHKPAGIRTNMIVGIIS
                     CLIMIVSLKIGNSNTGILNNADPARLAAQVISGIGFLGAGTILKNEDKVEGLTTAAGL
                     WAVAGLGLAIGYGLYILSIAAAIIIFVSLLLNDKRFF"
     gene            2641..3090
                     /locus_tag="VKN64_07395"
     CDS             2641..3090
                     /locus_tag="VKN64_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60102.1"
                     /translation="MKDNKNLKNNFILTLRKIARIWSIIILIISIIYLGGTFYNWITT
                     GTADPYAVEAAHPPIIFLGIIGLFIALKKEKLGGIITVLSGISHLGEEIYLWFFNQEI
                     PRLIIYIFSDELNYPATYYLTPYIVFLLILLPGILFWIIGSNLKNNI"
     gene            complement(3689..4138)
                     /locus_tag="VKN64_07400"
     CDS             complement(3689..4138)
                     /locus_tag="VKN64_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60103.1"
                     /translation="MTEEEKYKVEELLKSYQPLKSNVEIELNVLYPDYFLKSPSIVEC
                     KNELNDKGSITEKLAINNLDIPPKIKQKTQIILMVHSALNCLSIRDEYLVRQRFFQNK
                     NYTTIAEQINKHRDTVSRRVNKKILPKLIEIGILNAWELFEKTGPNH"
     gene            complement(4135..4395)
                     /locus_tag="VKN64_07405"
     CDS             complement(4135..4395)
                     /locus_tag="VKN64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60104.1"
                     /translation="MVTVNVHGIKYLCKLQGIFYKPLIIVYCPACKNKTMAVDFRNGK
                     GICSVCESKTTLDDLIVIWEEDKINISVDKMMRFNRGVGDNY"
     gene            complement(4383..4601)
                     /locus_tag="VKN64_07410"
     CDS             complement(4383..4601)
                     /locus_tag="VKN64_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60105.1"
                     /translation="MMEIAFESYTDKNKDYSIKLYKIQGEDLWGYTVEFRIKPVLNSR
                     LIISSDTRQEALGKAKNYLKFFRENWLQ"
     gene            complement(4602..5027)
                     /locus_tag="VKN64_07415"
     CDS             complement(4602..5027)
                     /locus_tag="VKN64_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60106.1"
                     /translation="MENNKKVQVTKIILPGRPITKKNHQRIIRKGGQSPSLISSRQYL
                     VYEKACLWELKKYEGQRFKGNKVQVKCLYWMPNYRSWPDLIGLIQATWDILEKADIID
                     NDQNIVSPDGSRIVGIDKENSRAEVYICEIGDHEYKEVG"
     gene            complement(5039..5440)
                     /gene="ssb"
                     /locus_tag="VKN64_07420"
     CDS             complement(5039..5440)
                     /gene="ssb"
                     /locus_tag="VKN64_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMA60107.1"
                     /translation="MINNIVLIGRLVRDPELRYTGSGIPVGNFTLAVERDFKNKQGIK
                     EVDYIKIVVWRKLAEVVAAHLSKGRLVAVNGSLQINKSQDKEGRSYINPEVNADKVQF
                     LDWGSQAPKINNLDKAEDVEIFDEDLNDIIF"
     gene            complement(5430..5567)
                     /locus_tag="VKN64_07425"
     CDS             complement(5430..5567)
                     /locus_tag="VKN64_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60108.1"
                     /translation="MSLSFNEKCILLTCLTKCELDLNFDKDKIQALKNKIKKEISEDD
                     K"
     gene            complement(5564..6139)
                     /locus_tag="VKN64_07430"
     CDS             complement(5564..6139)
                     /locus_tag="VKN64_07430"
                     /inference="COORDINATES: protein motif:HMM:NF022858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative helicase loader/inhibitor"
                     /protein_id="HMA60109.1"
                     /translation="MRKQDTLKLLKYINFYYGRKLDIPVEEELLEMLIQTWHDFLGGY
                     EYEMVKTAVKKLMINSEWPPTPGDIIKEIETIKLPKEAKLTAGEAWSLVLDIIGKYGT
                     SVNIKKAVDSLPFKVLEAARCVGGLQAIGRSSEDDTYLMNAFLQAYKNINLREREDKL
                     LPGSVRQEVKMLADRFKNKEQALLEDKGEGK"
     gene            complement(6136..7047)
                     /locus_tag="VKN64_07435"
     CDS             complement(6136..7047)
                     /locus_tag="VKN64_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60110.1"
                     /translation="MPRNGWIKLYRKIEDNPVWGDKPFSKGQAWIDLLLKVNHKKNKV
                     LIRSEIIEVAVGETITSIKKLCERWGWSNTKVNNFLNILEKEKMILCQKNTGKYTSIK
                     VLNYKDYQHSQDLTNIARASLEHTSSIPKTSPEHINKNDNNEKKNNKILLVRNLNKFQ
                     THIWKQLKYIDKAPKFDKDSIPYKAALHLRRLILKNNPRQQVPKADPFSLEKWSIEMD
                     RLNRLGPVGGDESKGYSWDEIAKIMSWCQDHYFWKKNILSAGKLREKVGRLEMDMKQD
                     HKSKPSIIKKQNEAFSSLYQKYKEAGE"
     gene            complement(7111..7917)
                     /locus_tag="VKN64_07440"
     CDS             complement(7111..7917)
                     /locus_tag="VKN64_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HMA60111.1"
                     /translation="MSSKGNDKVKNQLKEKKNNNPLGKKETIEMKVYQYIEKMKPEIE
                     KVLPKHIGVERMSRIAFTTVRTTSDLVNCSMGSLMGSVMQASALGLEPNLLGQCYIIP
                     YKNQATFILGYRGMINLARRSGEIQTLYAHVVKENDEFSYQYGLEPDLKHIPAEEERG
                     KIKGAYMVAKFKDGGYYFEYMPIADILKRRDKSPAAKSEYSPWNDMGIGYEEMVKKTV
                     IRHAFKYLPVSIEIMRNVELTDETQKDLKEFNKDDFIELKVDDISDRNVS"
     gene            complement(7907..8485)
                     /locus_tag="VKN64_07445"
     CDS             complement(7907..8485)
                     /locus_tag="VKN64_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60112.1"
                     /translation="MNNLKALRRVIKITQAQLGEEINLGQRDISKIETERREPTPEEI
                     KRISRYFKVDAQLIFPGIIKKAEAVKANKKCRVENIRIFDDGKGDLHWYIAIKLKNLS
                     KFLVKFEEFTDNTFIRIFNLRLNLADLVYKDLFNKSLVEIDISTSFHRYKNIMSDFIK
                     QKLDYDKKSTLDKDSVTSLFDVIDKGRERFEQ"
     gene            complement(8504..9454)
                     /locus_tag="VKN64_07450"
     CDS             complement(8504..9454)
                     /locus_tag="VKN64_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HMA60113.1"
                     /translation="MQAEVLVDTKDLTHEEWLEYRKQGIGGSDAAAAIGLGRWKSARM
                     VWAAKTGFLGEEKDNDAMRLGRDLEGYVAERFIEKRSEETGKEIKLWNVNQILRHSKH
                     KFMLANLDRRVLKESALLECKTTVDRKNEWKIDSFPEYYIPQLQHYLAVTGFEKAYLA
                     VLVLDKRWFNYYEVDRDDELIEYLIQEEKDFWESYVLTGVMPPIEEADLKNKIIDKLY
                     PEAKEGKIVHLDIGINELIEKRIDYKETENKFKNLRQEVETKIKDLLGEAELGFYQGQ
                     QVVKWRNINSSRFDSKKFRSEHPDLYSEYLNESSYRRLYI"
     gene            complement(9418..9687)
                     /locus_tag="VKN64_07455"
     CDS             complement(9418..9687)
                     /locus_tag="VKN64_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60114.1"
                     /translation="MKKLEIHNNIKKLRKRNNITQKELAEKLRITTSSVGMYERGARK
                     PSIEVLIKTANYFDISIDCLLFSKNLIDFKEAFKCKQRYWLILKI"
     gene            complement(9759..10028)
                     /locus_tag="VKN64_07460"
     CDS             complement(9759..10028)
                     /locus_tag="VKN64_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60115.1"
                     /translation="MKNDNQEGSEIIKKMLEESRSRYPLTLRAQDISEILGCKNKSQV
                     YGLVKKIPGAKKIESLGWRIPRDQFFAWYYFSQGETGYKNEKARL"
     gene            10424..10852
                     /locus_tag="VKN64_07465"
     CDS             10424..10852
                     /locus_tag="VKN64_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60116.1"
                     /translation="MDNDKMEKFIDFLEKSFRKSTIKSYSDLAEKADVDRSYLSMIIN
                     HKRPNPPSPEIIERLASVLDVSYNDMMKAAGYIKVYERPSDAEIETSKKVKILKGIFD
                     ELEIDYLEVIDEAKAKGLTPQDLKALIKISDQFKGLSKKD"
     gene            10963..11100
                     /locus_tag="VKN64_07470"
     CDS             10963..11100
                     /locus_tag="VKN64_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60117.1"
                     /translation="MVIVVEEKIDHEVIRIHKKKFKIIILNKSLPAEIKGKLYQRYRG
                     T"
     gene            11128..12294
                     /locus_tag="VKN64_07475"
     CDS             11128..12294
                     /locus_tag="VKN64_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMA60118.1"
                     /translation="MAHIQKIYKDDGSFRGYRAFVELNVNPLNGKRNRKSKLFNKLKT
                     AEKWMAEQINKAYEGSFINPSNITVGEYVLERWLNKHKKPQLAATTYDRYKGTIKTHI
                     IPTMGHIKLQELRPDHIEFYFSSKRENGRIDKPGGLSENTLKKHYVLLNEAMNRAIKL
                     KLIKYNPVQAVESPKFKKKKAAAMSEIHYKKLLEVLEDDRLMYTFVMTELMTGMRKSE
                     ILGLEWSDIDLEKGVIQINKRLVRTSDGVIHEKGTKTESSQRKIKISPKLIKLLKKHK
                     KKQQEFKLFFGPLYDNSKSFVFCKPDGRYYHPRTYNRRFNIALKKAGLPSKYTIHTLR
                     HTFATINLRNGIPAKIVQEMLGHSTISTTLDLYTHVDLEMQNEAVKKLEGKFDF"
     gene            complement(12427..12514)
                     /locus_tag="VKN64_07480"
     tRNA            complement(12427..12514)
                     /locus_tag="VKN64_07480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12478..12480),aa:Ser,seq:tga)
BASE COUNT         3932 a         2384 c         1876 g         4403 t
ORIGIN      
        1 gattgttatg ataaggaatt aattcttcag gttttattaa aattatttgg tttgatattt
       61 tcattgcacc ctcctacttt caaaaaatat tagcttaacc aaattttaga attttagatt
      121 attaaaaact ttttatataa aggattttct caagttttat tcaaatagtt tatatataaa
      181 aagagaaagc cgtttcattt atgactatat tattatatat aatatgaaac atacatcgta
      241 actatgtaag ttgtgtaaat ttgcatacta ttatatgaaa ggagcttaat atgataccag
      301 attatcaaaa aataatgttg cccttattga aattagctgg tgatgaaaaa acacacaagc
      361 ttagaaatgc catagaagaa ttggctgata actttgactt agaggaagaa gaaagaaaaa
      421 aactactacc aagtggaaat aaaaacatat ttgataatag agtggggtgg gcaaagactt
      481 atttgaaaaa agctggattg ctggaatatc ctaaaagagg gtattttaaa attactaaaa
      541 gaggtaaaaa agttcttcag aataatccag ataaaatcga taataacttt cttaaacaat
      601 ttggagaatt tcaaaaattt aaaaatacaa caaaatcaaa aaagaaaaaa aataaaaagg
      661 ggaaatcatc tgaaattttt gataaatata ccccagaaga agttatagaa cttggatata
      721 ctacgataaa taataattta gcctcgaatt tattagagaa aatacaagaa aacccccctg
      781 cattttttga aaaactagta gtagatttat tagtcaatat gggttatggt ggcacaatta
      841 aagatgcagg aagggcgatt ggcaaacatg gggatgaagg aattgatgga gtaataaaag
      901 aagatagatt aggcttagac attatataca tccaggccaa gagatggcaa aatactgtta
      961 gtagacctga aatccaaaaa tttgctggag ctttacaagg caaaaatgca aaaaaaggaa
     1021 tatttattac aacttctaaa ttctcaaaaa atgctattaa atatggaaag aagattaaca
     1081 gtaaaattat acttataaat ggtgaagatt tgactaagtt aatgataaaa tttgatgttg
     1141 gtgtttcaca atcatcatct tataaaatta aaaaagtaga taatgattat tttcttgacg
     1201 aatagatgcc gtgtttaatg taaaatttag aaacttcaaa taagagagga atcctgtcaa
     1261 gacagaaaac gggctgtttt aaaaatttaa atttagaatt agtaaatatt gaattagtta
     1321 taacaacaat atgtcatttt agtcaattat atatccccct atcttatatt cgcggtcgca
     1381 tctgttaacc tgccggcgcg gtgcggatct caggacctcc agagatttat accccctacc
     1441 cctattattt gttttctatt atatatttta ataaatgtaa ttttaaatta gaatgaaaaa
     1501 ttaattgaag gagtctagta aaaaatttag aataataatt attggagcca gtatatttat
     1561 tgattctact taataaataa taaaatcctt aaccccccaa aggttaagtt atcatatttc
     1621 aataaaatac tgactcctat attttttatg taatttcaaa ttttaaaaag agccatctgc
     1681 tcatcttgct gcagggctct tttaaaaact tattattatt aaatattgga taaatttaaa
     1741 aaaatcgttt atcatttagt aagaggctta cgaaaataat tatagcagca gcaatagata
     1801 atatatacaa tccataacca attgctagtc ctaaacctgc tacagcccac aaacctgctg
     1861 cagtagttaa tccttcaact ttatcttcat tttttaatat agtacccgca ccgagaaaac
     1921 caataccgct gatgacctgt gcagcaagtc ttgctgggtc tgcattattt aatatacctg
     1981 tattactatt accaatctta agtgaaacaa tcataattaa gcaagaaatt atccccacta
     2041 tcatattggt tctaatacca gctggtttat gtcttttctc tctatctatt ccaattaatg
     2101 ttgcaatcac tgctgttacc aataatttta ttatatcttc ccatactata tccataaaaa
     2161 caccttctct ttttctttat caattatata taatatataa aaatcctttt taatgatgac
     2221 taaaatcttg ttaattttaa attatatagt agcttcttaa taattattac ctaaaaaaga
     2281 gccaactgct cactatgaag cagggctctt tttaatattt ctcttattag ttctcgatat
     2341 tttagtaagt ttttatgact ttcttttttg tattctttgt catcatgcca gtatttctcc
     2401 cactcttctc atttttttaa tttcaggatt ccattatcca atttcaatct agtaccaata
     2461 catcggtaac catgcagaat acaataaagc ttgtgatcta atttatcagc ttctctcaaa
     2521 accttttttc cataaaaagg tgatagtaga agcatatcta tttttatgat ataattagag
     2581 aacaataaat tatttttgtt tataaatttt aacttgttta ataaagggat gagatataaa
     2641 atgaaagata ataaaaatct taaaaataac ttcattttaa ccttaaggaa aatagctcgg
     2701 atatggtcta taattatact gataatttca ataatttatt tgggaggtac tttttataat
     2761 tggataacta ccggaacagc tgatccttat gcagtagaag ctgcccatcc accaataata
     2821 tttttaggaa taataggatt gttcattgct ttaaagaagg aaaaactagg aggaattatc
     2881 actgtcttat cgggtatttc ccatcttgga gaagaaatat atttatggtt ctttaatcaa
     2941 gaaataccca ggctaataat ctatatattt tcggatgaat taaattatcc tgccacatat
     3001 tatttaaccc catatattgt ttttttatta atacttttac ctggtatttt attttggata
     3061 attggttcta atttaaaaaa taatatatga tgtaacttcc ttttttaata aaagtattct
     3121 ccttaccaaa atcaattaat tcattatcca tgttttctcc tatcctcttg agtcttcttc
     3181 atatgacagc ggtggcaggt agacttaaaa ttattaagat ccaaccgtct ggaccaatca
     3241 atcttaatag gaataacatg atccacttcg gtagcaattc gagtgcggtc cttttctaag
     3301 caatactcac ataaaggatt ttttgaaagt ttaattttcc tcaacttctt ccaagcagtc
     3361 gaattataaa acttgctaac cttcatgtcc ctttgttttt catcatagag tttattcctt
     3421 ttagagctta tcttcctctt gtggttctca caataagaaa tgcccggctc aactaacacc
     3481 gggcacccag gaaaagaaca tggttttttt aatttttttg gcatataaac tccttccaaa
     3541 aataatatag gcataagaaa aaccccatct attaagacgg ggcctgattc atattattta
     3601 aatatatttt cctactctaa ttatactaca ccttaaagga ataggcaata gtgaatagat
     3661 gctgaattta tgctgaaatg cttatatttc aatgattagg acctgttttt tcaaataatt
     3721 cccatgcgtt taatattcct atttcgatta atttaggcaa tattttttta ttaacccttc
     3781 tactgacagt atccctatgc ttatttattt gttctgcaat agtggtataa tttttatttt
     3841 gaaaaaaccg ctgcctcact aaatattcat ctcttataga aaggcagttc aatgcagagt
     3901 ggaccatcaa aattatttga gttttttgtt ttattttagg cgggatatct aaattattaa
     3961 tggccagctt ttctgttata gatcctttat cattaagttc atttttacat tcgacaattg
     4021 agggggattt aagaaaataa tcaggataca aaacgttaag ttcaatttca acattagatt
     4081 tcagaggttg ataacttttt agtagctcct ccactttgta tttttcttct tcggtcaata
     4141 gttatcacct acccctctgt taaatcgcat catcttatca acacttatgt ttatcttgtc
     4201 ttcctcccag ataacaataa gatcgtcaag agtggtcttg ctttcacata ctgagcaaat
     4261 ccctttgccg tttctgaagt cgacagccat agttttattc ttacaggccg ggcagtaaac
     4321 aataatcagt ggtttataaa agattccctg cagtttacat agatacttta taccgtggac
     4381 attcactgta accaattctc tctaaagaat ttgagatagt tttttgcttt gcccaaagcc
     4441 tcctggcggg tatctgaact gattattaac ctcgaattca agaccggttt aatcctgaat
     4501 tcaactgtat aaccccacag gtcctcaccc tgtattttat aaagctttat ggaataatcc
     4561 ttgtttttgt cagtataact ctcaaaagca atttccatca tttaaccgac ctccttatat
     4621 tcatgatccc ctatttcaca aatatacact tctgccctgg agttttcctt atctatgcca
     4681 acaatccggc tgccgtcagg gctgacaata ttttggtcat tgtcaataat atctgctttc
     4741 tcaaggatat cccaggtggc ctgaatcaac ccaataagat caggccagct tctataatta
     4801 ggcatccagt aaaggcattt gacctgtact ttgttaccct taaacctctg cccttcatat
     4861 ttttttaatt cccacagaca ggctttctca taaaccaaat actgccgaga ggaaataagg
     4921 gaagggctct ggccgccctt ccttatgatc cgctggtggt tcttttttgt aatcggccta
     4981 ccaggcaaaa tgatttttgt tacttggact tttttattat tttccatagt gaccgcctct
     5041 aaaagataat atcatttaag tcttcatcaa atatttcaac atcttctgcc ttatctagat
     5101 tattaatttt aggagcctgg ctgccccaat caaggaactg gactttatca gcattaactt
     5161 ccggatttat ataggacctg ccttctttat cctggctctt attgatttgg agtgaaccat
     5221 tcaccgcaac cagcctaccc ttgctgagat gtgcagcgac cacttcggcc agtttcctcc
     5281 agacaacgat ttttatataa tcgacttctt ttataccctg cttgttttta aagtcccttt
     5341 ctacagctaa ggtgaaatta cctacaggta ttccgctgcc tgtatatctc aactcggggt
     5401 ctctcactaa cctgccaatt aaaactatat tatttatcat cttcggatat ctcctttttg
     5461 attttatttt ttaaagcctg aatcttatcc ttatcaaagt ttaggtctaa ttcacacttg
     5521 gtaaggcagg tcaacaaaat acacttctca ttaaaggata agctcattta ccttccccct
     5581 tatcttctaa taaagcctgc tctttatttt taaagcggtc agctaacatt tttacttcct
     5641 gcctgacaga accgggcaaa agcttatctt ccctttccct taaattaata ttcttatagg
     5701 cctgcaggaa ggcgttcatc aaataggtgt cgtcctcgct gcttctccct attgcctgca
     5761 ggcctcctac acacctggct gcctccaata ctttaaatgg tagagaatca actgcttttt
     5821 tgatattaac actggtcccg tatttcccta ttatatccaa aaccaaagac caggcttctc
     5881 ccgcagtcaa ttttgcttct ttaggtagtt taatggtctc tatctctttt ataatatctc
     5941 ctggtgtcgg tggccactca ctattgatca ttaacttttt aactgctgtt ttgaccatct
     6001 cgtattcata acctcctaaa aagtcatgcc aggtttgaat taacatctcc aacagctctt
     6061 cctcaacagg tatgtccagc ttcctgccgt aataaaaatt tatatacttt aaaagcttca
     6121 gggtatcttg ttttctcatt cccctgcctc cttatatttt tgatataggc tgctaaacgc
     6181 ctcattttgt tttttgataa tagaaggttt ggatttatga tcctgcttca tatccatttc
     6241 cagccttccc accttttccc ttaacttacc agctgacagg atattctttt tccaaaagta
     6301 atggtcctga caccaggaca ttatctttgc tatttcatcc cagctatagc ctttgctttc
     6361 gtctcctccg acagggccta agcggttaag cctgtccatc tctatcgacc acttctctaa
     6421 agaaaaagga tcagcctttg ggacctgctg tctggggttg ttttttaaaa taagcctcct
     6481 taaatgcaga gctgctttat aaggaattga atccttatca aatttgggtg ctttatcaat
     6541 atattttagc tgcttccaaa tatgggtttg gaatttattt aaattccgaa caagaagtat
     6601 tttattattt ttcttctcat tattatcatt cttgtttata tgctcgggcg atgttttagg
     6661 tatgctcgag gtatgctcga gagatgctcg ggcgatgttt gtcaaatcct gggaatgctg
     6721 ataatcctta tagtttagca cctttataga cgtatacttg ccggtgtttt tttggcataa
     6781 aatcatcttt tctttttcca agatgtttag aaaattattt acttttgtat ttgaccagcc
     6841 ccatctttca cataattttt ttatggaagt aatagtttcc ccaaccgcca cttctataat
     6901 ttcactgcgg attaatactt tatttttttt atggttaact tttaacagca ggtctatcca
     6961 ggcctggcct tttgaaaatg gcttatcccc ccagacagga ttgtcctcaa ttttacgata
     7021 aagcttaatc cagccgtttc taggcatcta atcacctact ctaactgatc taaattaatt
     7081 agaacccggc cgctaagacc gggtatacaa tcatgaaaca tttctgtccg atatatcgtc
     7141 aactttcaat tcaataaaat catctttatt aaactctttt agatcctttt gggtttcgtc
     7201 ggtcagctcg acatttctca taatctctat gctaaccggc aggtatttaa aagcgtgcct
     7261 aatgactgtt tttttgacca tctcttcata gccaatcccc atatcattcc aggggctgta
     7321 ttctgatttg gcagccgggc ttttatcccg cctttttaat atgtctgcaa tcggcatgta
     7381 ttcaaagtaa tagccgccgt ctttaaactt agcaaccatg taagcgcctt taatcttgcc
     7441 tcgttcttcc tcagctggga tatgctttaa atctggttca aggccgtatt ggtagctgaa
     7501 ctcatcattt tcttttacca catgggcata aagggtctgg atctcgcccg accgtctggc
     7561 aaggttaatc attcccctgt atcccagaat aaaagtggcc tggtttttgt agggtattat
     7621 atagcattgt cccaaaagat tgggctctaa acccaaagca gaggcctgca tcacagaacc
     7681 cataagggat cccatactgc aatttactag atctgaagtg gtccggaccg tagtaaaagc
     7741 aatcctgctc attctttcaa ccccgatatg tttgggaagg actttttcaa tttctggttt
     7801 cattttctct atatattgat agactttcat ctcaattgtt tctttctttc cgagaggatt
     7861 attatttttc ttttctttca gctgattttt taccttgtca ttgcccttac tgctcaaatc
     7921 gttccctccc tttatctatg acatcaaaga ggcttgtcac agaatcttta tctagagtgg
     7981 atttcttgtc ataatcgagt ttttgtttaa taaaatcgct cattatattc ttatagcggt
     8041 gaaaggaagt tgagatatct atttcaacta aactcttatt aaacagatct ttatagacaa
     8101 gatctgctaa attaagccta agattgaata tacggatgaa ggtattgtca gtaaattctt
     8161 caaacttgac aagaaacttg cttaagtttt taagtttaat agcaatatac cagtgcagat
     8221 cacctttccc atcatcaaaa attcttatat tctctacccg gcatttttta tttgctttaa
     8281 cagcttctgc cttttttata atacccggga agattaactg tgcatctact ttaaaatatc
     8341 tgctaattct ttttatttct tccggagttg gttctcttct ttcagtttct atttttgaaa
     8401 tatcccgctg gcctaaatta atctcctcgc ccagctgggc ctgtgttatt tttataactc
     8461 tcctcaaagc ttttaaatta ttcattaatt aaccaccttt cttttatata tataaccttc
     8521 ggtatgaact ctcatttaga tattcactgt acagatccgg atgttcagac ctaaattttt
     8581 tgctgtcaaa tcggctgctg tttatatttc tccacttaac aacctgctgg ccctgataaa
     8641 aaccaagttc agcttctcct aataggtctt ttatctttgt ttctacttcc tgacgtagat
     8701 ttttgaattt attctcggtt tccttatagt ctattctctt ttcaattaat tcattaattc
     8761 caatatccag atgaactatc ttgccttctt tggcttccgg atagagttta tctatgatct
     8821 tgtttttaag atcagcttct tcgataggtg gcattacgcc agttaacaca tagctttccc
     8881 agaaatcttt ttcttcctgg attaagtatt caatcagctc atcatcccgg tcaacttcgt
     8941 agtaattaaa ccatctctta tccaaaacaa ggactgccag gtatgctttc tcaaagccgg
     9001 ttactgccag ataatgttgc agctgtggta tataatactc ggggaagcta tctattttcc
     9061 attcgttttt ccggtcaaca gttgtcttac attctaataa agcagattcc tttagtaccc
     9121 ggcggtctaa gttggcaagc atgaatttat gcttggaatg ccttaagatt tggtttacat
     9181 tccatagttt tatttctttg cctgtctctt ctgatctctt ttcaataaac cgttcagcca
     9241 catatccttc taagtcccgg cccagccgca tggcgtcatt atctttctct tctcctaaaa
     9301 acccagtttt agctgcccag accatgcgag cggacttcca cctgcccagg ccaatagctg
     9361 cagctgcatc actgcctcca atgccctgct ttctgtattc taaccattct tcatgagtta
     9421 aatctttagt atcaaccaat acctctgctt gcatttgaat gcctccttaa aatcaattag
     9481 atttttcgag aacaaaagac aatcaattga tatatcaaaa tagttggctg tttttattaa
     9541 cacctcaata ctgggttttc ttgcacccct ttcatacatt cccacagatg aagtagtaat
     9601 tctaagtttt tctgcaagtt ctttttgtgt tatattattt ctttttcgta attttttaat
     9661 attattgtga atttcaagtt ttttcatctt tccctccttg caattaataa gccttgctgc
     9721 tacaataaag acagatacat ttcaaaggtc cgcctctttc acaggcgggc tttttcattt
     9781 ttatatcccg tttctccttg actgaagtaa taccaggcaa aaaattgatc tctgggaatt
     9841 ctccatccaa gactttctat tttcttagct cccggaattt ttttaacaag tccatatact
     9901 tgtgatttgt tcttacaacc caatatttca gatatatctt gtgctctaag agttaatgga
     9961 tatctactcc ggctttcctc taacattttc tttattattt cactgccttc ttggttatca
    10021 ttcttcatta tttccctcct tttcatctcg gtctttttac tacccttgtt atttcaataa
    10081 tctcaccttc tttctttaat attttgtggt atatattcaa caatttggat ataaaaatac
    10141 ccctactaca actcaaatct actgctgttt attattataa cagcatttta aaaactttaa
    10201 ccatagtttg taaagagtta attatctctt taatcattgt tatagagagg tttataaaaa
    10261 gttttgcttt tgttatgttt tatattctga catttctgaa aaatataccc ttattatatc
    10321 actatgtagt tttttgtcaa taattatttt ttttattttt ataaatttta gttgacttat
    10381 gtaaaattaa gtagtataat tgcttcatca aggaggaatt tatatggaca atgataaaat
    10441 ggaaaaattt atagactttt tagaaaaatc atttagaaaa tctactatca agtcctattc
    10501 tgatttggca gaaaaagctg atgtagaccg ctcatacctc tctatgatca tcaaccataa
    10561 aagaccaaac ccaccctctc cagaaatcat cgaaagatta gctagcgttt tggatgtaag
    10621 ctataacgat atgatgaaag ccgctggtta tattaaggta tatgaaaggc cttccgatgc
    10681 agaaattgaa acctccaaaa aagttaaaat tctaaaagga atctttgatg aattagaaat
    10741 tgattacctt gaagtaattg acgaggccaa ggcaaaaggc ctaacccctc aagacttgaa
    10801 agctctaatc aagatatctg atcaattcaa aggtttatct aaaaaagatt aattcttttt
    10861 gcccatagca atatgggggt ttttttacat tttaagcgaa catatgtatt atcttgcgtt
    10921 catttttatg cttttccaaa tattaatagg ggggaattaa aattggttat tgttgtagaa
    10981 gaaaagattg atcacgaggt tataagaatt cataaaaaaa aatttaagat tattatttta
    11041 aacaaatctc ttccagctga aataaaaggc aaactgtatc aaaggtatag aggtacataa
    11101 caaatcttta gaaaggaggt gaaattaatg gctcatatac aaaagatcta taaagatgat
    11161 ggaagcttta gaggctatag agcttttgtg gaattaaacg ttaatccttt aaacggaaaa
    11221 agaaatagaa aatcaaagct atttaataaa ttaaagactg cagaaaaatg gatggctgaa
    11281 caaattaata aagcttacga aggctctttt ataaatcctt ctaacattac tgtaggtgaa
    11341 tatgtcttag aaagatggct taataaacat aaaaaaccgc agttagctgc cacaacttat
    11401 gacaggtata aaggcacaat aaaaactcac ataatcccca caatgggaca cattaaatta
    11461 caggaattaa gacctgatca tattgagttt tatttttcaa gcaaacgaga aaatggcagg
    11521 attgataaac caggcggact gtccgaaaat actctaaaaa aacattatgt tttattaaac
    11581 gaggctatga acagagcaat aaaattaaaa ctcataaaat ataatcccgt gcaggcagta
    11641 gaatccccaa aattcaaaaa gaaaaaagct gccgcaatgt cagaaataca ctacaaaaaa
    11701 ttgcttgaag ttttagaaga tgatagatta atgtacactt tcgtaatgac agaactcatg
    11761 accgggatga gaaaaagcga aatactaggt ttggagtggt ctgacattga tttagaaaaa
    11821 ggagtcatcc aaataaacaa aagattagtc agaacttctg atggggttat ccatgaaaag
    11881 ggcactaaaa cagaatcgag ccaaagaaaa attaaaatat cccccaagct tattaaactt
    11941 ctaaaaaagc ataagaaaaa acagcaggaa tttaaattat tttttggccc tctgtatgat
    12001 aacagtaagt ctttcgtgtt ttgcaagccc gatggaaggt attatcatcc caggacatat
    12061 aaccgtcgtt ttaatatagc cctaaaaaaa gcaggacttc cctcaaaata tacaattcat
    12121 actcttcgcc atacattcgc gactattaac ttaagaaatg gaatcccagc taaaattgtt
    12181 caagaaatgt taggacactc cactatatca actactcttg atttatacac tcatgttgat
    12241 cttgaaatgc aaaatgaagc agtaaaaaaa ttggaaggta aattcgattt ttagatcaca
    12301 ccctctataa aaaaattccc aaaccagaga aatcctctga atgtttggga acaatttggg
    12361 aacaatcata tttttctatc cgattttttg tatatcaacg aaaccgcaag gttccagtca
    12421 tatcaatggc ggagagaggg ggactcgaac ccccacacct ttcggcttca ccggatttca
    12481 agtccggctc cttagccaat tcggacatct ctccattttt gacacatctt aaattttact
    12541 ataatatcac tacattgtca agatatatga gatattcatt attttattga atatt
//
LOCUS       DATGCJ010000149        21826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_598765, whole genome shotgun sequence.
ACCESSION   DATGCJ010000149 DATGCJ010000000
VERSION     DATGCJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 21826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21826
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598765"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1132)
                     /locus_tag="VKN64_07485"
     CDS             complement(146..1132)
                     /locus_tag="VKN64_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA60119.1"
                     /translation="MKKLSLFVIVALTLTLVMGFSTMGMAQDDFKVGMVTDVGGLGDE
                     SFNDAAWRGLQNAKEDFGVEINVIESNTMTDYQPNLSSFAEQGYDMVWGIGFLFEDAM
                     GEVASMYPNVKFGIIDTVVDAPNVVSATFKEHQGSFLTGVVAGMRTESDKVGYIGGME
                     TPLIQKFEAGYRAGVKAVNPDAEVLIAYTGAFDDPQAGKELAFTQFDQGADVIYHASG
                     SCGIGVIKAANERGLYAIGVDSPQYHLAPANVLTSMIKKVDVAVYSEVEKLVNGNFES
                     GHHVYGLENEGVGIYRPQAENMLSQDILDAVDEYKEKIVNGEIEVPTSPDQL"
     gene            complement(1255..2049)
                     /locus_tag="VKN64_07490"
     CDS             complement(1255..2049)
                     /locus_tag="VKN64_07490"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMA60120.1"
                     /translation="MKLDIKGVKEHMNNNIIKNLKYFDSVTSTNELALEMKSENIQEG
                     VVIIGGEQTKGRGRLKRNWHSPQYLGLWFSIIVSPSKDLDRIPLLTLIGALSVHKAIK
                     NMGIKTDLKWPNDILYNNKKVCGVLSQFRSDGKEIDRVVVGIGLNVNQKEFPKELKDT
                     SISLRMIMEKIIKKEKLLAEILDNFSDFYQLFQNSEYDEIINSWKIEMSMLNKQITIN
                     TANGNKMKGKVVDISNKGELIIEKLNKERQKFIAGDVSIDKNSIRF"
     gene            2139..3287
                     /locus_tag="VKN64_07495"
     CDS             2139..3287
                     /locus_tag="VKN64_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMA60121.1"
                     /translation="MKLYKNILNNKYKFVIVVSIIFILAFSNLLIAQEEVIEETNETV
                     YVYQENIFADIAAEVDAGVVKITTITKRSQAEMQNPLYDDPFFRYFFGDQLPDLPENI
                     EGYGSGFIVTEGGFIVTNEHVIHEADQIKVNINGMDKSVPAEVIWTDYNLDLAVIKIN
                     VDQKLKTIPLGNSKEIRPGEWVIAIGNPFGFEHTVTTGVISALGRPIQIPTSQGQVRT
                     YKNLIQTDAAINPGNSGGPLLNIKGEVIGINTAVSAQGQGIGFAIPVNEVKEVVNDLQ
                     TKGEIIQPWLGVSVGQISPEVQEYFDLESNKGAIILDVFPGSPADKAGLKTYDIIKEI
                     DQERIENPDDVVEKIGQKEIEERVLIQVIRDGETEILFARIGRKPNKM"
     gene            complement(3435..3968)
                     /locus_tag="VKN64_07500"
     CDS             complement(3435..3968)
                     /locus_tag="VKN64_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HMA60122.1"
                     /translation="MKIKTRKLVFLSLLIALGVVLTRFLSLRLPLFGVEGIRIGIGSL
                     PMIFAGIVFGPMAGGIVGAITDLVGFWVSPMGAYMPHFTFTAFLTGFIPGFLMIYVFK
                     DRRTLTYILISIAVGQTITSIILVPYFIHILFGVPFRPLLIPRLIGQPITILVYSYLI
                     KILFKNEILNIFENGER"
     regulatory      complement(4029..4124)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4189..5859)
                     /locus_tag="VKN64_07505"
     CDS             complement(4189..5859)
                     /locus_tag="VKN64_07505"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMA60123.1"
                     /translation="MKSDIQIAQEADMQPIIEIAEQLGLTKDDLELYGDYKAKLKLDV
                     FNERKDTEDSKLVLVTAITPTPAGEGKTTTTVGLGQALNQRDKKAMIAIREPSLGPTM
                     GVKGGAAGGGYSQVIPMEDINLHFTGDIHAIGIAHNLLSAAIDNHIKQGNELGIDPTE
                     ISWPRVVDMNDRALREVVVGLGGSANGKPREDGYIITVASEIMAILCLANDLMELKEK
                     IGNIVIGYSYEGEPITAGDLNVQGAMTALLKDAIKPNLVQTLENTPALIHGGPFANIA
                     HGANSVMATKMAMKLSDITITEAGFGADLGAEKFFDIKSRYADLNPDATVLVATIRAL
                     KMHGGVAKDNLEEENLEALEAGMENLEKHVENINKFKVPLVVAINRFPTDTEAELELV
                     RKKCEEMGVRVALSEVWAKGGEGGLELADEIIDILDNEESNFEYLYSKELSIKEKIDK
                     IAKEIYGADGVDYSSAAEKQISTFEKQGYGELPICMAKTQNSISDKPELKGRPTDFTV
                     SVSQVTLSAGAGFLVPSTGPVLKMPGLPKRPSAEDIDIDENGKITGLF"
     gene            complement(6077..8086)
                     /gene="ftsH"
                     /locus_tag="VKN64_07510"
     CDS             complement(6077..8086)
                     /gene="ftsH"
                     /locus_tag="VKN64_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMA60124.1"
                     /translation="MNKFIKNIGFYLLLIAISILVAQFFMQQSPQTLENFSYSEMLQA
                     IENGNINTVTIIGNEKVTGTLEGQKFTVPIPPESIPVIMQELRAANVTIKTEPQPSAP
                     WWVSILGYVLPIIILIGAWFFLMQRMQGGGSKMMSFGKSKAKLNENKDDITFDDVANY
                     EEIKEELQEVVEFLKNPKKFARLGAEVPKGVLLVGPPGTGKTLMAKAIAGEAGVPFYF
                     ISGSDFVEMFVGVGASRVRDLFEKGKDNAPCIIFVDELDAVGRQRGAGLGGGHDEREQ
                     TLNQLLVEMDGFEPNEGIILMAATNRPDILDPALLRPGRFDRQVIVDRPDRKAREAIL
                     KIHVRKKPVDDSVDMEVLARRTPGFTGADMENLANEAAILAARRDKDVISMVEFDDAI
                     DRVIAGPQKKSRVINEKEKNLVAYHETGHALLGELLEHADRTHKVSIIPRGRAGGFTI
                     PLPEDDKNFMTKRELFDKITSLLGGRAAEDIFLEDISTGAQNDLERATKIARSMVTEY
                     GMSDKVGHLTLGQKHDEQVFLGRDISRSRNYSEQVAAEIDREVRSIIEYCYEKAQNLL
                     KENRDAVERIVAALKEKETLNAKQINKLIEDKELTPEDEYGNGNNKKEEDKETDQKED
                     TAQEADDTEGSKQGNEANEPAVEVVNAEENEPKKEDGQISSAENE"
     gene            complement(8169..8726)
                     /gene="hpt"
                     /locus_tag="VKN64_07515"
     CDS             complement(8169..8726)
                     /gene="hpt"
                     /locus_tag="VKN64_07515"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMA60125.1"
                     /translation="MEDEHIFNEDIAEVILTEEQIQTRIKEMGQEITEAYDEDDDIVM
                     LCVLRGAILFMSDLSRHINLPVVFDFMDVSSYEGTSSSGMVRIIKDLEENIEGRHVLI
                     IEDIMDTGLTLKHVIDLLKTREPADIKIATLLDKPERRVEKEVTADFNGFEVPDKFVI
                     GYGLDYKEKYRNLPYIGVLKEELYT"
     gene            complement(8727..10139)
                     /gene="tilS"
                     /locus_tag="VKN64_07520"
     CDS             complement(8727..10139)
                     /gene="tilS"
                     /locus_tag="VKN64_07520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMA60126.1"
                     /translation="MLDLKKRFKENLIDKDLVQHDDKILLGISGGPDSLTMLDLFSKI
                     KDELNLCLLVFHLNHSFRKEADQEAAFVKNLCEEKNIEVIIKKYDVPGYVKENNLSPE
                     EGAREIRFKFFQEIYERENIDRVSLAHNKDDLVETVLFNIFRGTGLRGLKGIEDKQVL
                     NGMEIIHPILIFYRSEILDYCEYNDLTPVFDPSNDSNLYSRNRIRNNILPMVEEQINS
                     NAKQVIARMTDTIKEDFDFLQKYSRKLLEELLVEKKLDKYILDLEKIRGLHPAIIKRI
                     INIIILKLKGSVDNFYYKHYSDIIRFIEENSTGDLLDLPDGIKLKISYNKLFIFQGTM
                     KQKEDFKKFIEEEGEYKLPYKQKLKVKITDEKLDWRNYKDNNYCLLDYDKVKFPLVVR
                     NRQKGDNFIPLGMQGHKKVKDYFIDEKVPDYKRDEIPIVFDSTGSLIWICGYRMDDRF
                     KLTNKSNKTIIINYQKEENK"
     gene            complement(10224..11117)
                     /locus_tag="VKN64_07525"
     CDS             complement(10224..11117)
                     /locus_tag="VKN64_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60127.1"
                     /translation="MFKKINEFRKSKVFKHFISQPLAVGALIVITLLFLGAVFAPLIA
                     PQNPYEMEKLSLSESLLPPVWMEGGQYPYILGTDVQGRGVFSTILYGSRTSFIVGFSV
                     VTISGLLGGGIGLFAGYFGGIFDSVVMRTADTVLSFSKTLIAMLLLGIFRQSSIVLVI
                     LAIVISDWVQYARTIRGEVLSVKEEEYVLASQAIGGTSMRTIFKHVLPNSISPLLVVG
                     AVDFGIAVMLEATLSFLGVGVPVTQPSLGMMISQGRNYLYAGKWWLIVFPGLTLMAIV
                     FSLNLISDWLRDEIDPKVKNI"
     gene            complement(11130..12098)
                     /locus_tag="VKN64_07530"
     CDS             complement(11130..12098)
                     /locus_tag="VKN64_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60128.1"
                     /translation="MSAYIGRRILQGILVLFLVSFIAFIIFQYLGDPVMTLAGRYATQ
                     AQRAEVREALGLNEPFYVQYFNFLKNALQGNFGMSYVSRVPVLELILERLPASIELAF
                     VAESFAVILGISFGVFVAANPKNTVSKFLMTGSLFGVSLPTFLVGILLIYLFSIVLGI
                     MPSSGRGNTVTIVGYWRSGLLTFDGWRHIILPAFTLGMFQMALLFRITRGAMLEVLGA
                     EYIRTAWAKGLKGKRVLFKHALRNALIPVVTMIGLQFGQLIGFAIVTESIFQWPGAGN
                     LLLNSLYESDFPVVATYVIIVALIVVMLNVIVDISYAFLNPKIRYK"
     gene            complement(12206..13750)
                     /locus_tag="VKN64_07535"
     CDS             complement(12206..13750)
                     /locus_tag="VKN64_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60129.1"
                     /translation="MSRNSSKILILIVVVSLLLAGSMSVLAQDQKVTVAVDAPPRTMD
                     PHGSDADSNLSVMSNFFEGLLQRNTKGELKPALATSWERVDRLTWRFELRKGVKFHNG
                     NDFTWEDVQYTFEERLENPEVSEFISFGSAIESVERVDGNDWVIDITTTDPIPYFVQN
                     LHQVFIVDKESTMERSQGDVGTKPIGTGPYVFEEWVKGSYLRMNANENYWEGEPEVQQ
                     AEIRPITEASTRLATISSGEVDILQGIPVTMYEAVENNNDLEVIARPSRRAIYLGLSS
                     DEDLPTSNLKVRKAMYMAINEPELIEKVMFGHATPAAQIPDPPTTGYSDQIERLEYNP
                     ERAKELLEEAGYGDGFKITLSAPNDRYVQDEQIASTVASYLSKVGIEVEVDAKPKSVY
                     FEEVMNNELDFYLIGWFDGSYDFGRSYSKLLHTIEEDTGYGTFNGARYTDPVADQLYE
                     VSKRVVDPSIRASILRDLNRLAMERVAVIPLHYQEDTYAQYKGRGIDFTPRSDTWIVF
                     KEMSFE"
     gene            complement(13838..15367)
                     /gene="hutH"
                     /locus_tag="VKN64_07540"
     CDS             complement(13838..15367)
                     /gene="hutH"
                     /locus_tag="VKN64_07540"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HMA60130.1"
                     /translation="MHYINGENLDVKKVSEIIFSKRKIRLSKKAENKVIKARETVERL
                     IDKGKVIYGLNTGFGKFSTKKISADKILKLQENLIISHAAGTGDYLDDEVVKTMILLR
                     LNALAKGHSGIRLSTLKKFIKMFNNEMIPLVPEQGSVGASGDLVPLAHMVLPLLGKGK
                     VKYKGEIIDSSEALKKVDIEPLKLASKEGLALINGTQMMASVEVLSIIKAQKLIKTAD
                     IALALSLEGLKGITGAFDEDIHKLRPHPGQMDSAANIRALTKGSQLMEEIDNNRVQDS
                     YTLRCGPQVHGASRDSLSHVIEIIEREMNSVTDNPLIFPDRNEVISGGNFHGQPLALS
                     SDYLGTAISELGNVSERRTAKLIDSSLNNGLEMFLTEHGGLHSGYMIAQYTSASIVSE
                     NKILANPSSTDSIPTSANQEDHVSMGSISARNLYKICSNVAQILAIELITAAQAVDLR
                     TDQPAEVMGEGTYLIYKKIRENVDYLDEDRILYPELLKIKEVIETGELLAALDNKFTI
                     Y"
     gene            complement(15446..16048)
                     /locus_tag="VKN64_07545"
     CDS             complement(15446..16048)
                     /locus_tag="VKN64_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HMA60131.1"
                     /translation="MTFANMSLKEFNSKLASDSPTPGGGSVAGLSAALGASLSSMVVH
                     LTKEGDLGKYEDELADHRAFALELIDKDALSFNKVMDAFKMDKDTDEAAQKRKEAIQS
                     ALYEASLTPMETIKLAKDVLEITEEVARKGNKNAISDAGVAALMALASVKSAAYNVYI
                     NTASLNDENKAKRLEEKTERLVKESEEIADEVERICKERI"
     gene            complement(16074..18086)
                     /locus_tag="VKN64_07550"
     CDS             complement(16074..18086)
                     /locus_tag="VKN64_07550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMA60132.1"
                     /translation="MYKYQDPTKKIKLPDTLPDKEEFRSDIRRAPKRELNLNENQIKL
                     ALKNALRYIPEHLHEEIAPEFLEELLTRGRIYGYRYRPKGEIKAKPLEEYEGNTVEGK
                     AIQLMIDNNLDFDVALYPYELVTYGESGQVCQNWMQYRLIKKYLQIMTEEQTLVVQSG
                     HPLGLFPSRPEAPRVIATNALMVGMFDNEEWFNKAAAMGVANYGQMTAGGWMYIGPQG
                     IVHGTYITLLNAGRLFLDIPEDEDLSGHLFISSGLGGMSGAQAKAVEIAGGVGVIAEV
                     DKSRIDTRYEQGWVSKVSADLDQIFDWVDKYRKEGKSISIAYHGNIVDLLEYVNENDI
                     NLELLSDQTSCHAAYSGGYTPVDYTFQEGRKLLNNEPDKFKQEVDRSLKRHFEVLKDL
                     VDKGAYFWDYGNSFMKAVFDAGVKEIAKNNEDTSEGFIFPSYVENIMGPICFDYGYGP
                     FRWVCLSGKDEDLRKTDQAAMEEIDPTRRAQDRDNYHWIKDAEENDLVVGSKARILYA
                     DAEGRVDIALKFNELVRKGEIGPVMLGRDHHDVGGTDSPFRETSNIKDGSNIMAEMSI
                     HCFAGNAARGMSMVVESNGGGVGIGKAFNGGFGLVLDGSKRVDNIIKNALDWDVNGGI
                     ARRAWARNENALETAEKWNQKNLGRGQMTIPNIADEELIEELVDNK"
     gene            complement(18100..19353)
                     /gene="hutI"
                     /locus_tag="VKN64_07555"
     CDS             complement(18100..19353)
                     /gene="hutI"
                     /locus_tag="VKN64_07555"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HMA60133.1"
                     /translation="MLLIKNIGQLVTVGGNKPKSGSEMKEINVIEDAYLVIKEDEIFD
                     FGKSKALKEQNQYDKVIDAQNKVVMPGFIDPHTHLVFGGDRSYEFKMRLRGVPYIDIL
                     KNGGGILSTVNETRKSSYQDLLDVSNKRLDRFLSYGVTTVEGKSGYGLDKTTEIKQLE
                     IMKELNEREDIDVVSTFLGAHAVPKEYNKSDQYIDYIIQEVMPEVVENELAEFCDVFC
                     DEGAFSYQESEKLLKAADKMGLKLKIHADEIINTNAAQLAGKLKAVSAEHLLKASREG
                     LNSLRENDVLAVLLPLTAFSLQEEYADAKQMIEMDIPVALATDLNPGSSYSQSIPLLL
                     SLAVMYMDMKPEEAITALTLNAAAALNREEQVGSIQKGKKADLLIIDAPNYNHLFYNF
                     GVNLVEKVYKNGKLVFDNSLENRKF"
     gene            complement(19353..20255)
                     /gene="ftcD"
                     /locus_tag="VKN64_07560"
     CDS             complement(19353..20255)
                     /gene="ftcD"
                     /locus_tag="VKN64_07560"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HMA60134.1"
                     /translation="MAELFECIPNFSEGRDQEKIEKIVEPFKKTKGIKLLDYSADKDH
                     NRLVVTLLGGAEELKKSVHAAIGIAVDVIDMNQHKGEHPRMGAVDVVPFTPVKNVEME
                     DAVKLANEVAKKAAEEYDLPIYLYEEAASTDQRKNLADIRRGEYEGFADKIKKDEWKP
                     DYGPQAVHPTAGATVIGARMPLVAFNINLDTDDLEIANKIARKVRHSSGGLRFVKAIG
                     IDLSERNIVQVSMNMTDYTQTSLYQAFEMVKFEAKRYGVNVIGSELIGLLPAQALFDV
                     AEYYLGLEDFESEQIMENRLLEDI"
     gene            complement(20332..21603)
                     /locus_tag="VKN64_07565"
     CDS             complement(20332..21603)
                     /locus_tag="VKN64_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60135.1"
                     /translation="MEKSNKKVIYSWVLYDWANSAFATTILAAVLPIFYNDVAAANLP
                     DNLATSYWGYTQAIAMLIIALITPVLGAVADYSKSKKIFMKMFVFIGVTGTALLFFII
                     EGRYLMASIFFIFANIGFSGGNIFYDSFLPIITKKDKIDYVSSMGYAAGYLGGGILLL
                     INLVMIMRPGLFGIANTTLATRIAFITVAVWWVVFSIPAFINLPESRVGLGKFTLGPY
                     IKQGFHRVANTFKHVKKYKELFKFLLAFWIYNDGIGTIIRMATIYGREVGIGQNDLIG
                     ALLLTQIVGIPFTLIFGKLAEKIGAKRGIYLALTVYLIITILGYNLDSALDFWILAGL
                     VGTVQGGAQALSRSLYGALIPENKSAEFYGFYGISSKFAAITGPFVFALVGQITGSSR
                     YGILAVASFFIIGIILLYTVDVEKGVEESVS"
     gene            21732..>21826
                     /locus_tag="VKN64_07570"
     CDS             21732..>21826
                     /locus_tag="VKN64_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA60136.1"
                     /translation="MKIGLVTDSTCDLKSNIIEKFNIEIVPLTVH"
BASE COUNT         6653 a         4700 c         3282 g         7191 t
ORIGIN      
        1 agatcatact aacacctcta atccttattt atatgctaag ttatattata tacaaaatct
       61 agtattatta caattttacc cattaaaaaa tgcccggttt tacccgggca ttttgcttgc
      121 ttatttctgt tttatttata acgctttata gttggtcagg acttgtagga acttcgattt
      181 caccattaac aattttttct ttgtattcat caacagcatc tagaatatct tgtgaaagca
      241 tattttcagc ttgtggacgg tagattccga caccttcatt ttctaaacca tatacatggt
      301 gtccagattc aaagttacca ttaacaagtt tctcaacctc actatatact gctacgtcaa
      361 ccttcttaat catactggtt aatacgttag caggagctaa gtgatactgt ggtgagtcaa
      421 caccaattgc atatagtcct ctttcgttag cagctttaat tacaccaata ccacaggaac
      481 cagaagcgtg ataaattacg tctgcaccct ggtcaaactg ggtaaaggca agttctttac
      541 ctgcttgtgg gtcatcaaaa gcacctgtat aagcaattaa tacttctgca tcaggattaa
      601 ctgcttttac gccagctcta taaccagctt caaacttctg aatgagtgga gtttccatac
      661 caccaatata tcctacctta tcactttcag ttctcatacc tgctacaaca ccagttaaaa
      721 atgaaccctg atgttcttta aaggtagcag agacaacatt aggtgcatct acaacggtat
      781 caataatacc aaacttaaca ttgggataca tggaagcaac ttcacccatt gcatcttcaa
      841 ataagaaacc tataccccat accatgtcat aaccttgttc agcaaaactt gataggttag
      901 gctggtagtc agtcatggta ttagattcaa ttacattgat ttcaacacca aagtcttctt
      961 tagcattctg gagacctctc caggcagcat cattaaatga ttcatcacct aagccaccaa
     1021 cgtcagtaac catacctact ttaaaatcat cttgagccat tcccatggtt gaaaagccca
     1081 taactaatgt gagtgttaat gctacaatta caaataaact taatttcttc aaatcaactt
     1141 ccccctttta aaatttttta taattagttg ttggtaatag aaatattatt cccaactttt
     1201 tcagcgtcaa atatattatt caagattaat catcttattc cttcttttga atagttaaaa
     1261 tcttattgaa ttcttgtcaa tactaacatc accggcaata aatttttgcc tctccttatt
     1321 taacttttcg ataattagct ctcctttatt tgaaatatcc actactttac ctttcatttt
     1381 attaccattt gcagtattaa tcgttatttg tttattcaac atagacattt ctattttcca
     1441 actatttatt atttcatcat actcactatt ttggaataat tgataaaaat cagaaaagtt
     1501 atctaaaatc tctgccaaga gtttctcttt cttaattatt ttttccatga tcatccttaa
     1561 tgaaatagaa gtatctttta attccttggg aaattctttt tgatttacat ttaaacctat
     1621 cccaactacc actctatcaa tttctttccc atcactccta aattgcgata ataccccaca
     1681 tactttttta ttattatata aaatatcgtt aggccatttc aaatcggttt tgattcccat
     1741 atttttgatg gctttatgca ctgacaaagc ccctattaaa gttaaaagtg gaatcctatc
     1801 caaatcctta gatgggctta caattatgct aaaccataag cctaaatatt gaggagaatg
     1861 ccaatttctt tttaaacggc ctctgccttt tgtctgttct cccccaatga taacaacgcc
     1921 ttcttgtata ttttcagact tcatttctaa ggctaattca ttagttgaag tgactgaatc
     1981 aaagtatttt aaattcttta ttatattatt attcatatgc tctttaacgc cttttatatc
     2041 aagtttcaaa acggttcaca tccttttgac atctatggaa tattataata taattttagg
     2101 tacaaagcaa aaaaagaaaa tggaaggagt gtaaaattat gaaactatat aaaaatattt
     2161 taaacaataa atataaattt gtaattgtag tatctattat ttttattcta gctttttcga
     2221 atctgttaat agcacaagaa gaagtgatag aagagaccaa cgaaacggta tatgtgtacc
     2281 aggaaaatat atttgcagat atagcggcag aagttgacgc gggcgttgta aaaattacaa
     2341 ctattactaa aagaagtcag gcagagatgc aaaacccctt atatgatgat ccatttttta
     2401 gatacttctt tggggatcaa ctgccggatc ttcctgaaaa tattgaaggt tatggcagcg
     2461 gttttatcgt aacagaaggc ggttttattg tcaccaatga acatgttatc catgaagcag
     2521 atcaaatcaa ggtaaatatt aacggtatgg ataaatcagt accagccgaa gtaatctgga
     2581 ccgattataa tcttgacctg gctgtcatta aaattaatgt cgatcaaaaa ttgaaaacga
     2641 tacccttggg aaactcaaag gaaataaggc cgggagaatg ggttatcgca attggcaacc
     2701 ccttcggttt tgaacacaca gttacaaccg gtgtaattag tgccttaggt cgaccgattc
     2761 aaatccccac ttcccaggga caagttagaa cctataaaaa cctgattcaa actgatgcag
     2821 ctatcaatcc gggtaatagt ggtggccctt tacttaatat aaaaggtgaa gtaatcggta
     2881 ttaatacagc tgttagtgcc cagggacagg gaattggttt tgcgatacct gttaatgaag
     2941 taaaagaagt agtaaacgac ttacaaacta aaggggaaat tatccagccc tggttaggag
     3001 tatctgtcgg acagatcagt ccggaagttc aggaatactt tgatttagaa agtaataaag
     3061 gtgctataat attggatgtc ttccccggca gcccagctga taaagcaggt ttaaaaactt
     3121 atgacatcat caaagaaata gatcaagaaa gaattgaaaa tcctgatgat gtggtagaaa
     3181 aaatcggtca aaaagaaatt gaagaaagag tgctaattca agtaattaga gatggggaaa
     3241 cggaaatact atttgcacgg ataggtagaa agccaaacaa aatgtaaatt aatatataag
     3301 aaaagtagaa gcggctaata tataaagata aaatactata acaatatttt aaaaatagtt
     3361 aagagtaaaa agcgtgtgaa tcattatttt agattcacac gcttttttta gatattaaat
     3421 ttgcaggtgc aatattatct ttcgccattt tcaaaaatat ttaatatctc atttttaaat
     3481 agtattttga tcaaataact atatactaaa atggtgatgg gttgaccaat cagcctcggt
     3541 attaacaatg gcctgaatgg tacaccgaat aagatatgga taaagtaggg aactaaaata
     3601 atggaggtta tggtctgtcc tacagctatt gaaatcaaaa tatatgttaa ggttcttcta
     3661 tctttaaaaa cataaatcat tagaaatccc ggtataaaac ctgttaaaaa agctgtaaaa
     3721 gtaaaatgag gcatatatgc tcccatcgga ctaacccaaa aacctaccaa atccgtaata
     3781 gctcctacaa tacctccagc cattggacca aaaactatac cggcaaagat catcggtaat
     3841 gatccaatgc caattctaat tccttccact ccaaataatg gcaaacgaag acttaaaaat
     3901 cttgttaaaa ccacacccag agctatcagc aatgacaaaa atactaactt tctcgtttta
     3961 atcttcattt gatcattcct ctctttgggt gatgacagat atctgcacca aagagaagac
     4021 tctttgatga cagcggacgc gaatttcaca ccgcagtttt ttaacattcg ttctaaagca
     4081 acgtcccatc tttagacact ttaacgcgcg aaatctactc tatcatctaa ttgttattta
     4141 taatttaata tatttttatt aaattaaata gtaattattt aacaattttt agaacagtcc
     4201 agttatctta ccattttcat cgatatctat atcttcagcc gaaggtctct tcggtaaacc
     4261 gggcatctta agcacaggtc cggtcgaagg tactaaaaag ccggccccgg ctgaaagggt
     4321 tacctgggag actgataccg taaagtcggt cggtctgccc ttaagctctg gcttatcaga
     4381 tatggagttt tgggtcttgg ccatacagat cggtaattca ccataaccct gtttttcaaa
     4441 ggtactgatc tgtttctcgg ccgccgaact gtaatctact ccatctgctc catagatctc
     4501 tttagcgatc ttgtcgatct tctctttaat agaaagttct ttactgtata gatactcgaa
     4561 gttgctctct tcattatcta aaatatctat tatctcatcg gctaactcca ggccgccttc
     4621 gccgcctttc gcccatactt ccgataaagc aactctaact cccatttctt cacatttctt
     4681 tctaactaac tctaactccg cttcggtatc ggttgggaac ctattgatag ccaccactaa
     4741 aggtacctta aacttattga tgttctctac atgtttctct aagttttcca tacctgcttc
     4801 taaggcctct aagttctctt cttctaagtt atccttagct actcccccgt gcatcttaag
     4861 agccctaata gttgcaacca atacggtcgc atccggattt aaatcagcat aacgggactt
     4921 gatatcaaag aacttctctg cccctaaatc ggccccaaaa ccggcctctg ttatcgtaat
     4981 atcggataac ttcatcgcca tcttggtcgc cattacactg ttggctccat gggcaatgtt
     5041 ggcaaaagga ccgccgtgga tcaaggcagg tgtgttctct aaggtctgga ctagattggg
     5101 tttgatggca tctttgagta aggctgtcat cgctccctgt acatttaggt caccggctgt
     5161 aatgggctct ccctcataac tatagccgat cacgatatta cctatctttt cttttagttc
     5221 cattaagtca tttgccaaac ataagatagc catgatctca gaagccactg taatgatata
     5281 gccgtcctct ctgggcttac cgttggctga accacccagg ccgactacta cttctcttaa
     5341 ggccctatcg ttcatatcta ctacccgcgg ccagctgatc tcggtcggat cgattcctaa
     5401 ttcattaccc tgtttgatgt ggttatcgat cgctgctgag agcaggttgt gagcgatacc
     5461 gatcgcatgg atatcacctg tgaaatggag attgatatct tccatcggga ttacctggga
     5521 atagccgccc ccggctgctc cccctttaac acccatggtc ggtcctaaag aaggttcacg
     5581 gattgctatc atggcctttt tatctctttg gtttaaggcc tggcctaaac cgacggtagt
     5641 ggtagtttta ccttcaccgg ccggagtagg tgtaatagcg gtaactaaga ctaacttact
     5701 gtcttcagta tcttttcttt cattaaagac atcaagcttt aacttggcct tgtagtcacc
     5761 atatagttct aggtcgtctt tggttaagcc taactgttca gcaatttcaa tgatgggctg
     5821 catgtctgct tcttgtgcta tttgaatatc acttttcaaa atatctcccc cttttatttt
     5881 ataaaaaata taatattaac agtttactta tttcttgatt aaattttaaa atcctttaaa
     5941 taatttaatt aaattaaata aatataaaat aatgccagct attaaactgg cattaataat
     6001 aattagaact gatatgctct aatttactac atattattgg cttttttaat tttaaattat
     6061 atttaatatt gattaattat tcgttttcag ctgaactgat ttgcccatct tcttttttag
     6121 gttcgttttc ttccgcatta acaacttcaa cggccggttc attggcctca tttccttgct
     6181 ttgaaccttc agtatcgtct gcttcttggg ctgtatcctc tttttgatca gtttctttat
     6241 cttcttcttt tttattattt ccgtttccat attcatcttc tggggttagt tctttatctt
     6301 caattaattt attaatttgt tttgcattta aagtttcttt ttcttttaag gcggcaacaa
     6361 ttctttccac agcatctcta ttttctttca atagattttg ggctttttca taacagtatt
     6421 caattattga tcttacttct ctatcaattt ctgcagctac ttgctcactg tagtttctac
     6481 tgcgagatat atcgcggcct aagaatacct gttcatcatg tttttggcct aaagtcaagt
     6541 gaccaacttt atcactcatt ccgtactcag ttaccattga acgagctatt tttgtagctc
     6601 tttccaggtc attttgagcc ccagtactaa tatcttctaa aaagatatct tcggcagctc
     6661 taccacctaa aagagaagtt attttatcaa ataactctcg cttagtcata aagttcttat
     6721 catcttccgg taaaggaatg gtaaatccac ccgctctacc tctcgggata attgaaactt
     6781 tgtgagtacg atctgcgtgt tccagtaatt cccctaagag tgcatgtcca gtttcatgat
     6841 aagcaactag attcttctct ttttcattaa taactctgct tttcttttgg ggaccggcaa
     6901 ttacacgatc tatagcatca tcgaattcaa ccattgaaat gacatcttta tctcttctag
     6961 ccgcaagaat cgcagcttca tttgcaaggt tttccatgtc agcaccggta aaaccaggag
     7021 ttcttcttgc gagaacttcc atatctacac tgtcatcaac cggtttcttt ctaacatgta
     7081 ttttaagaat tgcttcacgt gctttacggt caggacgatc tacaataacc tgtctatcaa
     7141 atctaccagg cctcagtaag gcagggtcta aaatatcagg tctattggtc gcagccatta
     7201 agatgatacc ttcattaggt tcaaaaccat ccatctcaac cagtaactgg ttaagggttt
     7261 gttctctttc atcatggccg ccgcctaaac cggctccacg ttgtctacct acagcatcta
     7321 actcgtcaac aaaaataata cagggtgcat tatctttacc tttttcaaaa aggtctctaa
     7381 ctctagaagc accaacacca acaaacattt caacaaagtc cgaaccactt ataaagtaaa
     7441 atggtacgcc agcttctccg gcaattgctt ttgccatcag tgttttacca gttccaggag
     7501 gtcctactaa aagaacacct ttaggaactt cagctcctaa tcttgcaaac tttttagggt
     7561 tctttaagaa ctcaactacc tcctgtaatt cttccttgat ttcttcataa tttgcaacat
     7621 catcaaaagt tatatcatct ttattttcat ttaatttagc cttgcttttc ccaaacgaca
     7681 tcatcttact tccgccgccc tgcatcctct gcattaggaa aaaccaagca ccaattaaaa
     7741 ttattatcgg aagaacataa cctaaaatgc taacccacca gggtgcacta ggttggggtt
     7801 ctgttttgat agtcacattg gcagctctaa gttcttgcat tattaccgga atcgattcgg
     7861 ggggaattgg tactgtaaac ttttgaccct caagagtacc ggtaactttt tcattaccta
     7921 taatcgtaac agtatttata ttcccatttt caatcgcttg caacatttct gaataactaa
     7981 agttttctag agtttgagga gactgctgca taaaaaattg cgcaacaagt attgaaattg
     8041 ctattagtag tagataaaat cctatatttt ttataaactt attcaaatta cagttcctcc
     8101 cttcctacaa atatacccat acagtaagat attataacat aagttaatta ctttgtaaat
     8161 gtattaaact acgtatacag ctcttctttt aatactccta tatatggtaa atttctatat
     8221 ttctctttat aatctaatcc ataaccaatc acaaatttat caggtacttc aaacccatta
     8281 aaatcagcgg ttacttcttt ttcaaccctt ctctcgggtt tatccaagag agtagcaatt
     8341 ttgatatcgg ctggttctct tgtttttaat aaatctataa catgttttaa agttaaacca
     8401 gtatccatga tatcttctat tattaagaca tgtcttcctt caatgttttc ttctaagtct
     8461 tttattattc gaaccatgcc agaagaagaa gtaccttcat aactcgatac atccataaaa
     8521 tcaaaaacca ccggtaaatt aatatgtctg gataaatcag acataaataa gattgctccc
     8581 ctcaaaacac ataacataac tatgtcatca tcttcatcat aagcttctgt tatttcttga
     8641 cccatttctt taattctggt ttgaatctgc tcttcagtta agattacttc agcaatatct
     8701 tcattaaaaa tatgttcatc ttccatttat ttattttcct ccttctggta attaattata
     8761 attgttttat tgcttttatt tgttaactta aacctgtcat ccattctata tccacatatc
     8821 caaattaaac tccctgtact atcaaacaca attgggatct cgtccctctt gtagtcggga
     8881 actttttcat ctataaaata atctttaacc tttttgtgac cctgcattcc caggggtata
     8941 aaattatcac ctttttgacg gtttcgaact acaagtggaa atttaacttt atcataatca
     9001 agcaaacaat aattattatc tttataattg cgccaatcta acttttcatc tgtaatcttc
     9061 acttttagtt tttgtttata tggtagctta tactcccctt cctcctcaat gaatttttta
     9121 aaatcttctt tttgtttcat agttccctga aatataaata atttattata agagatcttt
     9181 aattttatac catcaggcag atctaataaa tcgcctgtac tattctcctc tataaatcta
     9241 attatatcac tataatgctt ataataaaaa ttatctacac taccttttaa ctttaaaatg
     9301 ataatattta taattctttt aataatagca gggtgtaatc cccttatttt ctccaaatct
     9361 aaaatatatt tatctaattt tttttctacc aataattctt ctaaaagctt tcgactatat
     9421 ttttgcaaaa agtcaaagtc ttctttaata gtgtcggtca tccgggcaat aacctgtttg
     9481 gcattcgaat ttatttgctc ttctaccata ggtaaaatat tatttctaat cctgtttcta
     9541 ctgtataaat tactgtcgtt actgggatca aagacagggg taagatcatt gtactcacag
     9601 taatctaaaa tttcagacct ataaaaaatt aagatgggat gtataatttc catcccatta
     9661 agaacttgtt tgtcttcaat gccctttaaa ccgcgcaggc cggtacctct aaaaatattg
     9721 aaaagaactg tttctactaa atcatctttg ttgtgggcca aacttaccct gtctatattt
     9781 tctctttcat aaatttcttg gaaaaattta aacctgattt cacgggcccc ttcttccggc
     9841 gaaagattat tctctttcac ataaccaggc acatcatatt tttttataat aacttcaata
     9901 tttttctcct cacaaagatt ctttacaaag gctgcttcct ggtcagcttc ttttcgaaaa
     9961 gaatggttta aatgaaaaac aagcagacat aaatttaact catctttaat tttggaaaac
    10021 aaatccaaca tagttaggga atctggtccc ccagatatcc ctaataatat tttatcatca
    10081 tgttgtacta aatctttatc tattaaattt tccttaaatc tcttctttaa atcaagcatg
    10141 ggaaatatta tttccttcca tatttacttc taattaaata ttcaatatta aattaatatc
    10201 tcctttttaa gtagaaaaat gaattaaata ttctttacct tgggatctat ctcatctctt
    10261 aaccagtctg aaattaagtt taaactaaat acaatagcca tcagggtcaa accagggaat
    10321 actatcaacc accatttacc ggcataaaga taatttcttc cctgcgagat catcatgcct
    10381 aaagaaggct gggtaacagg tacccctacc cctaaaaagc tcaaggtagc ctcaagcatg
    10441 acagcaatac caaaatcaac ggcacccact actaataaag gtgaaatgga attgggcagg
    10501 acatgtttga aaatagtcct catactggtg ccgccaatcg cctgactggc cagcacatac
    10561 tcttcttctt taacagatag cacttcaccc cggatcgtcc tggcatactg cacccagtcg
    10621 gaaatcacaa tagccaaaat aactaagact atactgctct gtctaaaaat tcctaataag
    10681 agcatcgcaa ttaaagtttt agaaaaggat aaaacagtat cagcagtccg catcacgaca
    10741 gaatcaaaaa taccaccgaa ataaccggca aaaagaccga tccctccccc cagaaggcct
    10801 gaaatagtta ccacactgaa tccaactata aaggaagttc ggctgccata taaaatagta
    10861 ctaaataccc ctcttccctg gacatcagta cctagaatat aaggatactg accaccttcc
    10921 atccaaacag gaggtaacag agattcagat aaactcaatt tctccatttc ataagggttt
    10981 tgaggtgcaa tcaggggagc aaaaactgcc cctaaaaata atagggttat tactattaaa
    11041 gctcctactg ccagtggttg acttataaaa tgtttaaaca cctttgattt tctaaactca
    11101 tttatttttt taaacatatc atcatcacct tatttatacc ttattttggg atttaagaaa
    11161 gcgtaagaaa tatcaactat gacattcaac atcacaacga ttaacgctac gataatgaca
    11221 taagtagcaa ccacgggaaa atcactctca tagagagagt ttaaaagtag attaccggct
    11281 cccggccact ggaagatact ctctgtaaca atagcaaaac cgattaattg gccgaactga
    11341 agccctatca tagttactac cggtattaaa gcattgcgga gggcatgttt aaataaaact
    11401 ctctttcctt ttaaaccttt cgcccaggca gttctaatat actctgcccc taatacctct
    11461 aacattgctc cccgcgtgat cctaaagagg agcgccattt gaaacatacc caaagtaaag
    11521 gcaggtaaga taatatgtct ccaaccatca aatgttaaaa gcccacttcg ccagtaacca
    11581 actatggtaa ctgtattacc tctaccagaa gagggcatga tacctaaaac aatcgaaaat
    11641 aaatatatta ataaaatacc aactaaaaat gtaggtaatg aaacaccgaa taaagaaccc
    11701 gtcattaaaa acttactaac cgtattctta ggattagcag ccacaaagac accaaatgat
    11761 attcccagta ttactgcaaa agactctgcc acaaaagcca gttcaatact agccggtagt
    11821 ctttctaaaa taagttccaa cacaggaacc cgggaaacat acgacattcc gaaattaccc
    11881 tgtaaagcat tctttagaaa attaaaatac tgtacataaa acggctcatt taaaccaagt
    11941 gcttctctaa cttcagcccg ttgggcctgg gttgcatacc taccggctaa agtcataacc
    12001 ggatctccca gatattgaaa gataataaat gctataaaag aaactaaaaa tagtactaat
    12061 ataccctgta atattcttct accaatgtaa gcactcatta ttgatactcc cttctaattc
    12121 acttacatgt ttatttttat ataagaaagc atggagctat tgagtagctc catgcaatct
    12181 tatttaacat tactttttgc tttctttatt caaaggacat ttctttaaat actatccagg
    12241 tatcggacct tggagtaaaa tcaattcctc tacccttata ttgtgcataa gtatcttcct
    12301 gatagtgaag tggtattact gctactcttt ccatagctaa tctatttaga tctcttaaaa
    12361 tactagctct tatggaagga tcaacgactc tcttacttac ttcatatagt tggtcagcta
    12421 ctggatctgt atatcttgca ccattaaatg taccgtaacc agtgtcttct tctatagtat
    12481 gaagtaattt agaataagat ctaccaaagt catatgatcc atcaaaccag ccaattagat
    12541 aaaagtctaa ttcattattc ataacttctt caaaatatac tgatttaggt tttgcatcta
    12601 cctcaacttc gatgcctact ttagataaat aactggccac agttgaagca atctgctcat
    12661 cctgtacata tctatcatta ggagctgata gagttatctt gaagccatca ccataaccgg
    12721 cctcttctaa tagttccttc gctctttccg gattatactc tagtctttca atctgatcac
    12781 tataaccagt agtaggtgga tcaggaatct gagcggccgg tgtcgcatga ccaaacatta
    12841 ccttctcaat taattcaggt tcattaatag ccatatacat tgccttacgt actttcagat
    12901 tagaagtagg taaatcttca tcactactca aacctaaata aattgcacgc cttgaaggtc
    12961 tggcaataac ttctaagtca ttattatttt caacagcttc atacattgta acaggaattc
    13021 cctgtagtat atcaacttca ccactgctaa ttgttgctaa acgggtggaa gcttctgtta
    13081 taggtctaat ttcagcttgc tgaacttcag gttcgccttc ccagtaattt tcatttgcat
    13141 tcatgcgcag ataggaacct ttaacccatt cttcaaatac atatggtcca gttccaattg
    13201 gtttagtacc aacatcaccc tgacttcttt ccatggttga ttccttatca acaataaata
    13261 cctggtgtaa gttctggaca aagtatggaa taggatcggt agtagtaata tcaattaccc
    13321 aatcatttcc atccactctt tcaacagatt cgatggcaga accaaaagaa ataaattctg
    13381 aaacctctgg gttttcaagt ctttcttcaa aagtgtattg aacatcttcc caggtaaaat
    13441 cattaccgtt gtggaactta actccttttc tcaattcaaa tcgccaggtc aagcggtcaa
    13501 ctctttccca actggtagca agggcaggct ttaattcacc ctttgtattc ctctgtaata
    13561 aaccttcaaa aaagttgctc attacagaaa gattagagtc tgcatctgaa ccatgggggt
    13621 ccattgttct aggtggagca tctacagcca ccgtaacctt ttgatcttga gctaaaacac
    13681 tcatactccc tgctaataaa agactaacta caacaatcaa aataagaatc ttgcttgaat
    13741 ttctactcat tactctcctc cttaatttag aattattatt tcctcttatt atataagaaa
    13801 aagtggtgtc tttcaaactt atcttacctt tactatctta ataaattgta aacttattat
    13861 ccaaagctgc tagtagttca ccagtttcaa taacttcttt aatctttaat aattcaggat
    13921 ataagattct atcttcatct aagtaatcca cattctctct aatcttctta taaattagat
    13981 aggtaccttc ccccattact tccgctggtt gatctgttct aagatcaacg gcctgggcgg
    14041 cggtaattaa ttcaatagca agaatttggg ccacattact gcaaatttta tataggtttc
    14101 tagcactaat cgaacccatg ctgacatggt cctcctggtt ggcagaagtg ggtatcgaat
    14161 cagtactgga agggttagcc agtatcttat tttcagaaac gatagaggca gaagtatatt
    14221 gggctatcat ataacccgaa tgtagaccac cgtgctcagt taaaaacatt tctaagccat
    14281 tattaagact gctgtcaatc aatttagcgg tccttctttc cgatacgtta cccagttcac
    14341 tgatggctgt ccctaaatag tcactgctta aagcaagagg ttgaccgtga aaattcccac
    14401 ctgaaatcac ttcatttcta tctgggaaaa tcaagggatt atctgtaacc gaattcatct
    14461 ctctttcaat aatctctatt acatgggaaa gactgtcgcg ggaagccccg tgtacctggg
    14521 gcccacacct taaggtataa gaatcctgga ccctattatt atctatctct tccatcagtt
    14581 ggctgccttt agtaagggct cttatattag cagcggaatc catctgcccg ggatgggggc
    14641 gcagcttatg aatgtcttca tcaaaagcac ctgtgatccc ctttagacct tccagagaga
    14701 gggcaagggc aatatcagcc gttttaataa gtttttgagc ttttataata cttaatactt
    14761 cgaccgaagc catcatttgg gtcccattga ttaaggctaa accttcttta ctagctaatt
    14821 ttaaaggttc aatatctact ttttttagag cttcactgct atctataatc tcacctttat
    14881 acttaacctt acctttacct aaaaggggta aaaccatatg agctaaggga accaaatctc
    14941 cactagcacc aaccgaaccc tgttcaggta caagtggtat catctcatta ttaaacatct
    15001 taatgaattt ctttaaagta gatagtctaa tacccgaatg tcctttggca agggcattaa
    15061 gccttaataa aatcatcgtt ttcactactt catcatcaag gtaatctccg gttccagccg
    15121 catggctgat aattaaattc tcttgcagtt tcaatatctt atctgctgag atcttttttg
    15181 tactaaattt accaaaaccg gtatttaatc cataaataac tttaccctta tcgattaatc
    15241 tttcaacggt ctctcttgct ttaataacct tattttcggc ttttttggaa agccttatct
    15301 ttcttttaga aaaaattatt tcacttactt tttttacatc caaattttca ccgttaatat
    15361 aatgcaaaat atctcctctt ttccttaaag tttacagaaa aaaagaaggg cttatgaaac
    15421 taagtccttc ttttgaaata ttattttaaa ttctttcttt acaaattctt tcaacttcgt
    15481 ctgctatttc ttcactttct ttaactaatc tttctgtttt ttcttccaat cttttggcct
    15541 tgttttcgtc attgagagaa gcagtattta tatagacatt ataggcggca cttttaacac
    15601 tagctaaagc cattaaggct gccacccccg catcagaaat tgcattttta ttaccttttc
    15661 tagctacttc ttctgtaatc tctaaaacat ctttagccaa cttaatagtt tccattggtg
    15721 ttaaggaagc ttcatataag gcagattgaa tcgcttcttt tcttttctgg gctgcctcat
    15781 cagtatcttt gtccatctta aaagcatcca ttactttatt aaagcttagc gcatctttat
    15841 cgattaattc taaagcaaaa gctctgtgat ctgccagctc atcttcatac ttacctaaat
    15901 cgccttcttt ggttaagtga actaccatag aggacaagga agcccccagc gcggcactta
    15961 aaccggctac actccctcca cctggagtag gtgaatcact ggcaagttta ctattaaatt
    16021 ctttaagact catatttgca aaggtcatct ttagcctcct aattttttat ttcttactta
    16081 ttatcaacta actcttcgat caattcttca tcagctatat tgggaatagt catctggcct
    16141 ctacccagat ttttttgatt ccatttttca gcagtttcta aggcgttttc atttctagcc
    16201 caggcccggc gggctattcc accatttaca tcccaatcca gggcattttt gattatatta
    16261 tctacccgct tggaaccatc taagaccaaa ccgaagccgc cgttaaaagc tttaccgatt
    16321 cctacgcccc caccattgct ctctactacc atactcatac cgcgggccgc atttcctgca
    16381 aaacagtgaa tagacatctc ggccataata ttgcttccat ctttaatatt agaagtttct
    16441 ctaaagggtg aatcagtgcc acctacatca tggtggtctc gccctaacat gacaggacca
    16501 atttcccctt ttctaaccag ttcattaaat ttgagggcaa tatcaactct accctctgca
    16561 tctgcatata gtattcttgc tttggaacca accaccagat cattttcttc cgcatcttta
    16621 atccagtggt agttgtctct atcctgagcc cttcgggtcg gatcaatttc ttccatagcg
    16681 gcctgatcgg tctttcttaa atcttcatct ttaccactta aacataccca gcggaaagga
    16741 ccatagccgt aatcaaaaca gattgggccc ataatatttt caacatagga aggaaagata
    16801 aagccttccg aagtatcctc attatttttg gcaatttctt taacaccagc atcaaagaca
    16861 gccttcataa agctgttccc ataatcccag aagtaggctc ccttatctac taaatccttt
    16921 aacacttcga aatgtctttt taaacttcta tctacttcct gcttgaattt atccggttcg
    16981 ttatttaaca attttctacc ttcttggaag gtataatcaa ccggggtata accgcctgaa
    17041 taagcagcgt gacaggaggt ttgatctgat aatagttcta aatttatatc attttcattt
    17101 acatattcta aaagatctac aatattgccg tggtaagcta tggaaattga cttgccttct
    17161 ttacggtact tatcaaccca atcaaatatt tgatctaggt cagctgatac ttttgatacc
    17221 catccctgtt cataacgggt gtcaattctt gatttatcaa cttcagcaat aacgccgact
    17281 cccccggcta tttcaacggc tttcgcttgg gcaccgctca tgcctcctaa acctgagcta
    17341 ataaataagt gtcctgagag gtcctcatct tccggaatat ctaaaaatag tcggccggca
    17401 ttaagtaatg tgatataggt accgtgtaca ataccctggg gaccgatata catccagcct
    17461 ccggctgtca tctgtccata gttagctact cccatagcag cggctttatt aaaccactct
    17521 tcattatcaa acataccaac cattaaagca ttggtggcaa ttactctggg tgcttcggga
    17581 cgagatggaa ataggccgag cggatggccg gactgaacaa ctaaagtctg ttcttcggtc
    17641 ataatctgca aatatttttt gattaaccga tactgcatcc agttctgaca aacctgaccg
    17701 gactcaccgt aagtaactaa ttcatatggg tataaggcga catcaaaatc taaattattg
    17761 tctatcatca gttgaatggc cttaccttct acagtatttc cttcatactc ttctagaggt
    17821 ttagccttta tctcaccttt aggtctataa cgataaccat aaatcctgcc ccttgttaaa
    17881 agttcttcta aaaattccgg tgcaatctct tcatgaagat gttcgggtat gtatcttaag
    17941 gcatttttta gggctaattt aatttggttt tcatttaaat ttaattctct cttaggtgcc
    18001 ctcctgatat cagaccggaa ttcttcttta tccggcagtg tatccggtaa cttgattttt
    18061 ttggtcgggt cctgatactt atacataaaa tttccccctt taaaattttc tattttctaa
    18121 actgttatca aataccaact tgccattttt atataccttc tccaccaagt tcacgccaaa
    18181 attataaaat agatggttgt aatttggggc atcgatgatt aatagatcag cctttttacc
    18241 tttttgaata ctacctactt gctcttcgcg atttaaggcg gccgccgcat ttaaagttaa
    18301 agcggtaata gcctcctccg gtttcatatc catatacata accgctagtg ataaaagcag
    18361 tggtatagat tgagaataac tactgcccgg gtttaaatca gttgccaagg ccacaggaat
    18421 atccatctcg atcatctgct tggcatcagc atactcttcc tggagagaga aagcagttaa
    18481 aggtagcaag acggctaaaa catcattctc ccttaaacta tttaaccctt cccgagaggc
    18541 ttttaagaga tgttcggctg aaacagcctt aagcttaccc gctaactgag cggcattggt
    18601 attgattatt tcatccgcat gaatttttaa tttgagtccc atcttatcgg ctgcttttag
    18661 taacttctcg gattcctgat aggaaaaagc cccctcatca cagaatacat cacagaattc
    18721 agccagttca ttttcaacaa cttccggcat tacctcttgt ataatataat ctatgtattg
    18781 atcagattta ttatactctt ttgggaccgc atgtgcacct aaaaaagtgg atactacatc
    18841 tatatcctct ctttcattta attccttcat tatttcaagc tgctttattt cagtggtttt
    18901 atccaatcca taaccacttt tgccctccac agtggtaaca ccatagctta aaaacctatc
    18961 caatctctta ttggaaacat ctaaaagatc ttgataggaa ctttttcgtg tttcattgac
    19021 agtacttaag attccgcccc cattttttaa gatatctata taggggactc ctcttaacct
    19081 cattttaaat tcataggaac ggtctccccc aaaaaccagg tgggtatgag gatcgataaa
    19141 gcccggcatt accactttat tttgggcatc tataactttg tcatactgat tttgttcttt
    19201 taatgcttta cttttaccaa aatcaaatat ctcatcctct tttattacta aatatgcatc
    19261 ttctattaca ttaatttcct tcatctcaga acctgatttt ggtttattac ccccgaccgt
    19321 gactaactgc ccgatatttt taattaatag cattatatgt cctccagtaa tctgttctcc
    19381 ataatctgtt cactctcaaa gtcttctaaa cccaaataat attcagccac atcgaataag
    19441 gcctgggccg gtaataaccc gattagctca ctaccgatca catttacccc atacctttta
    19501 gcctcaaatt taaccatctc aaaagcctga taaagtgagg tttgggtata atctgtcata
    19561 ttcatagaaa cctgtacaat atttctttcc gagaggtcga tcccaattgc cttcacaaac
    19621 ctcaagccgc cactggagtg tcttactttt ctggctattt tattggcaat ttctaaatca
    19681 tcagtatcta agtttatatt aaatgcaacc aaaggcattc ttgcccctat tacagtggca
    19741 ccggctgtgg gatgaactgc ctggggtcca taatccggtt tccactcatc ttttttgatt
    19801 ttatcggcaa aaccttcata ttcacctctt ctaatatcgg ctagattttt tctttggtcg
    19861 gttgaggccg cttcttcata aagatagatc ggtaaatcat actcttcagc tgccttttta
    19921 gctacttcat tggccagttt aaccgcgtct tccatctcaa catttttgac gggagtaaaa
    19981 ggaaccacat caaccgcccc catgcgggga tgttcacctt tatgctggtt catatctatt
    20041 acatcaaccg caattccaat cgcggcatga acagattttt taagctcctc tgctcctcct
    20101 aacaaagtaa ctaccaacct attatggtcc ttatcagctg aatagtctaa gagtttgatc
    20161 ccttttgttt ttttgaaagg ttcaacaatt ttctcgattt tctcttgatc tctaccctca
    20221 ctaaaattcg gaatacactc gaaaagttct gccatacaaa tacccctttt catttaaaat
    20281 tttctaataa tattataaca attcttttaa taaattgtaa gtatgattaa gttaagaaac
    20341 tgactcttct accccttttt ctacatctac tgtatacaat aaaatgatac cgattataaa
    20401 aaaggaagcc actgctaaaa taccatatct agaagaacct gttatctgtc ccactaaagc
    20461 aaatacaaag ggacccgtga tagccgcgaa cttacttgaa atcccgtaaa aaccataaaa
    20521 ttcggcactc ttattctctg ggattaatgc cccatataag gacctgctta aggcctgggc
    20581 acctccctgt acagtaccta ctaagcccgc taatatccaa aaatctaaag ctgaatcaag
    20641 attgtatcct aaaatggtaa tgattaaata aaccgttaaa gcaagataga tgcctctttt
    20701 tgcccctatc ttttctgcca gtttaccaaa tattaaagta aatgggatac ccacaatctg
    20761 agttaaaagg agggcgccga ttaaatcatt ttgaccgata cctacttcgc gaccatagat
    20821 agtggccatc ctgataattg tcccgatacc gtcattatag atccaaaaag ccaatagaaa
    20881 tttaaaaagt tctttatatt tctttacatg tttgaatgta ttagctaccc tatgaaaacc
    20941 ctgtttaata tagggtccca gggtaaattt gccgagacca acccttgatt ctggtaagtt
    21001 aataaaggcg ggaatagaaa aaacaaccca ccagaccgct acagttataa aggcaattct
    21061 agtagccagg gtagtatttg cgattccaaa aagccccggt ctcataatca taacaagatt
    21121 aattaagagt aagataccgc cgcctaaata accggctgca tatcccatgg aagaaacata
    21181 gtcaatttta tctttttttg taataattgg taaaaatgaa tcataaaaga tattgccccc
    21241 ggaaaagccg atattggcaa aaataaaaaa tatagatgcc ataagatagc gtccttcgat
    21301 aataaaaaat aataaggccg ttccggtaac tccgataaat acaaacatct tcataaatat
    21361 ttttttagac tttgaataat cggccaccgc ccctaaaacc ggtgtgatta aagcaataat
    21421 taacatggca atcgcttgag tatagcccca ataagaagtt gccagattgt ccggcaaatt
    21481 agcggctgcc acatcattat aaaatatagg taagacagct gctaaaatag tagtagcaaa
    21541 ggctgaatta gcccagtcat ataaaaccca ggaatatata acctttttat ttgatttctc
    21601 caattttatc ctcacctacc tcataaatat attattcttg aagaaaattt atttacctct
    21661 tttagattct gcttgatatc ttttttgatt aaagatataa tttagtcaaa attagtaggg
    21721 gtgaaatttt catgaaaatt ggtttagtaa ctgatagtac ctgtgattta aaatcaaata
    21781 ttatagaaaa attcaatatt gaaatagtac cactgaccgt tcattt
//
LOCUS       DATGCJ010000150         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_464552, whole genome shotgun sequence.
ACCESSION   DATGCJ010000150 DATGCJ010000000
VERSION     DATGCJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464552"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..479
                     /locus_tag="VKN64_07575"
     CDS             18..479
                     /locus_tag="VKN64_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60137.1"
                     /translation="MNKVRPGQDKEHLRKEYRDIIFKSGYYEDLSHGAQVIYIYLIDL
                     FIENCDHEYKEQIKGGYVEFKDLIDEYGEELFDEFNTDNNYKETKFSMLKAIKELEDN
                     KLITVVLIKDSDNKPIILIYIQDINLSYKEKVLDFMLKKMFYNEYKKRKQH"
     gene            538..978
                     /locus_tag="VKN64_07580"
     CDS             538..978
                     /locus_tag="VKN64_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60138.1"
                     /translation="MKRSSLLKLKFYLVRCLVKGSWERIIGVCKLEKDVSGEFLYKHK
                     EMPLLIGEKELARILYLSRRKAKSLMDKPYFPTLEIKGDRYITKENLICWLELDRISK
                     DDVDLNYNGRPDTEKQLIQTAFFKGIEIGINSHKDWELNRKVVG"
     gene            980..3178
                     /locus_tag="VKN64_07585"
     CDS             980..3178
                     /locus_tag="VKN64_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HMA60139.1"
                     /translation="MELVNNIFEEIKNQLDIVKVYEDETGYQVISRDEQVQVRCFLPG
                     HVDRNPSCSINREQGVWFCHRCKKSGSVIDLVMATQGFDNPITAVNYLAEKNGIDTDS
                     YQLSELFYEKKEKEYIYTDLEGAPLHKTIRKPDKSFSQQRFANGKWEWGLEDTKLVLY
                     NLPALNKVIKSGETIFIVEGEKDAGLLTKKGLIATTNALGAGKWKESYTNTLIGGNVV
                     IIPDNDLPGKNHAEHIAEKLYNKVQSLKILTLPGLGVKGDVTDWLLQSGTKEELLDLA
                     EKSLPYESEKFNKKIIINNNDFLPTPLARDLIIKENRKGKFYRYVAEKEVFYYYDNKG
                     YWRVINTKYVKKIIRNQLLSYNNKWDKKHYIEEVLEALKNYVLHEVNLNLFNVNIYHE
                     NVNINPYNVYINTIQGMFDWQKGIILPYDPNYYSQFQLNVKYDPKKECPLWIESLKQW
                     IPERESHLFLQEFAGYCLIPDTSFHKALFLVGSGSNGKSTFLNVLAALFGTENLSNIP
                     LHRLTDKFETVNIQDKLVNICPDIDPTYLKKTGILKTIIAGESVRGEYKYGPPFSFIP
                     VVRLIFSTNELPMSRDHSEGWYRRFEIIRFPNEFKKSDPKFDPDLEVKLKGEISGIFN
                     WAVEGLRRLKRNGNFTQSNSIIQAKREYEIENDSVASFIDENTELEPDGYEICSYVYN
                     EYKAYCQEANLSPVKRRKFTSRIKTLGFKSKVTKLYGKSERCYYGFTLKN"
     gene            3729..4376
                     /locus_tag="VKN64_07590"
     CDS             3729..4376
                     /locus_tag="VKN64_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA60140.1"
                     /translation="MILGISGSGRPDGITSQSVKFLLEATGEKYQYISLSDKKINGCI
                     GCLHCAEDATCKQKDDWNEIGEKMLKADAIVFGAPNYYNNLNALAHACLERTFCFRHR
                     ETFKLAGKLGIAIGVDDSKEDPLITELLTKYMKSNHMAIVDEIQLKGYSQCYTCGYGE
                     NCAAGNVVNDHGFIDEIQKWHLPQDFSEQNRRIYELHKAGKILGSILRNQKNEGS"
BASE COUNT         1661 a          628 c          918 g         1308 t
ORIGIN      
        1 gggaaaggaa gatgtaaatg aacaaagtca gaccgggtca agataaagaa catctaagaa
       61 aggagtacag agatataata tttaaaagcg gctattatga agacttgtca catggggcac
      121 aggtgatata tatctattta atagatttat ttattgaaaa ttgtgatcat gaatataagg
      181 aacaaattaa aggtggttat gtagaattta aagatttaat agatgaatat ggggaagaac
      241 tgtttgatga atttaatact gataataatt ataaagagac taagtttagt atgttaaaag
      301 ctatcaaaga attggaagac aataaattaa taactgtcgt tcttattaag gattctgaca
      361 ataagccaat aattttaata tatattcagg atataaatct atcttacaaa gagaaggtat
      421 tagattttat gcttaaaaag atgttttata atgaatacaa aaaaagaaaa caacattaac
      481 actgatagga cccagccctt aggggctggg aatttctcta aaccaagggg gaagtttatg
      541 aagagatcat ctttgctgaa gttaaaattt tatttagtta gatgtttggt taaaggtagc
      601 tgggaaagga taataggggt ttgtaaactt gaaaaagatg tgagtggaga atttctttat
      661 aagcacaaag agatgccact gttgatcggg gagaaagaac tggccagaat tttatatctt
      721 tcacgaagaa aagcaaaatc tttaatggac aaaccatatt ttcccacatt agaaatcaag
      781 ggagatagat atataactaa agaaaattta atttgttggt tagagttaga tagaataagt
      841 aaggatgatg ttgatctaaa ttataatggg aggccggata ctgaaaaaca gttaatacaa
      901 acagcttttt ttaaagggat tgagatagga atcaatagcc ataaagattg ggaattaaat
      961 cgaaaggtag ttggttaatt tggagttggt caataacatt ttcgaggaga taaaaaacca
     1021 gcttgatatt gtaaaggttt atgaagatga aaccggctac caggtgataa gcagagatga
     1081 gcaggtccag gtaaggtgtt ttctgccggg ccatgttgac cgcaaccctt cctgcagtat
     1141 taatagagag cagggagtct ggttttgcca ccgctgtaag aaaagtggtt cagttatcga
     1201 tttagtaatg gccacccaag gttttgataa ccctattaca gcagtcaatt atctagcaga
     1261 aaagaatggt attgatactg acagctatca gctttcagaa ttattttatg aaaaaaaaga
     1321 aaaagaatat atctataccg acctggaggg agctcctctt cataaaacaa tccgaaagcc
     1381 tgataaaagc ttttcccagc aaagatttgc aaatggaaag tgggaatggg ggttagagga
     1441 tactaagctt gttttataca acttacctgc ccttaataaa gtcatcaagt caggtgaaac
     1501 catttttatt gtagaaggtg aaaaagacgc tggcttgtta acgaaaaagg gccttatagc
     1561 gaccacaaat gccctgggag ccggcaagtg gaaggagagc tatacaaata ccttaatcgg
     1621 aggtaatgtc gtaataattc ccgataatga cctacctgga aaaaatcatg cagaacatat
     1681 tgcagaaaaa ttatataata aagttcagag tttgaaaata ttaacactac ccggtttagg
     1741 tgtaaaaggt gatgtaactg attggctgtt acaaagtggg actaaagaag aactattgga
     1801 ccttgctgaa aaaagtcttc cctatgaatc tgaaaaattt aataaaaaga taattataaa
     1861 taataatgac ttccttccta cccctttagc gagggatctg attattaaag aaaatagaaa
     1921 aggcaaattt tatcgatatg tagctgaaaa ggaagttttt tattactatg ataataaggg
     1981 ctactggcgg gttattaata ctaaatatgt taaaaagatt atcagaaatc agttattaag
     2041 ctataacaat aagtgggata aaaaacatta tatagaagag gttcttgaag ctttaaaaaa
     2101 ttatgtacta catgaggtca acctcaacct ctttaatgta aacatttatc acgaaaatgt
     2161 aaacattaat ccctataatg tatacattaa taccattcaa ggtatgtttg actggcaaaa
     2221 aggaataata cttccctatg acccaaacta ttacagccag tttcagttga atgttaaata
     2281 tgatcctaaa aaggaatgtc ccttgtggat tgaaagcctc aagcagtgga tacccgaaag
     2341 ggaatcccat ctgtttctac aggagtttgc cggctactgt ctgattccgg acacttcttt
     2401 tcataaagcc cttttcttag tgggcagcgg cagtaacggt aagagcacct ttcttaatgt
     2461 cctggcagcc ttatttggga cagaaaacct ttctaacata ccactgcacc ggttaacaga
     2521 taagtttgag acagttaata tccaggacaa gctagttaat atatgtcctg acattgaccc
     2581 tacttactta aagaagacag gtatcttaaa gactattata gctggtgaat cagttagggg
     2641 agagtataag tatggtccac ctttcagctt tattccagtt gtccggttaa tattttccac
     2701 caatgagctg cccatgtcca gggaccatag tgagggttgg tacagaaggt ttgaaattat
     2761 cagattcccc aatgaattta aaaaaagcga tcccaaattc gatcctgatt tagaagttaa
     2821 gttaaaagga gagatttctg ggatcttcaa ctgggcggtt gaggggctta gaagattaaa
     2881 aagaaacggc aacttcactc agtcaaattc cattattcag gctaaaagag aatatgaaat
     2941 agagaatgat agtgtggctt cgtttattga tgagaatact gaattagagc cggacggata
     3001 tgaaatatgt tcctatgttt ataatgaata taaagcctac tgccaagaag ctaatttaag
     3061 cccagtaaag agaagaaaat ttacttcccg cataaaaacc ctggggttta agtcaaaggt
     3121 gactaaactt tatggaaaat ctgagagatg ctactatgga tttacattaa aaaattagat
     3181 aaaaaaagaa gtgtaaccgt gaaactcctt gggccatcta aattcagagg ccctcttttt
     3241 ttgcggttac actttggtta cacttctcaa aaataagtgt aaccatgaaa taccttgtcg
     3301 ctctaaggct tatgggtttg ggttacgaag ttacacttct ttttaaaaag ttagctaaga
     3361 aattaaaata atataaaagt ttcttattag aagtgtaact ctgtaaccgg tatactgcag
     3421 ttattgatat aaaagggatt agaacggtta cactttatta agccaaagtg taactttctg
     3481 gagagaagtg taactagatt aataaacaga agttttaaag ggtagataat ataaaaatat
     3541 tggaattaat agaaaaaact aaaagtattc tgatatgatg tgaatgtcaa ataattagat
     3601 tttttgcaga atattgaact ttataatagg ttgaataatt tatttattca tttacatcca
     3661 ttaagagctg tgctattata aaaataacta agataataaa tatccgagtt atgaaaggag
     3721 gcacatcagt gattttagga atcagtggaa gtggacgacc agatggaata acttcacagt
     3781 ctgttaaatt tttactggaa gcaacaggtg aaaagtacca atacatatca ttgtcagaca
     3841 aaaaaataaa tggttgtata ggttgtcttc actgtgctga agatgccaca tgcaaacaaa
     3901 aggacgactg gaatgaaata ggagaaaaga tgttgaaagc agatgcaatt gtctttggtg
     3961 caccgaatta ttataataac ttaaatgcat tagcacatgc ctgtctggaa cgcacatttt
     4021 gttttagaca ccgggagaca tttaaacttg ccggaaaact tggtattgct attggagttg
     4081 atgacagtaa agaggatcct ttaataactg aattgcttac aaaatacatg aagtctaatc
     4141 atatggcgat agtggatgaa attcagttaa aaggttattc gcagtgttat acatgtgggt
     4201 atggagaaaa ttgtgctgct ggaaatgttg taaatgatca tggatttatt gatgaaattc
     4261 aaaaatggca tttgccacag gatttttcag aacaaaatag gagaatttat gaacttcaca
     4321 aagcgggcaa aatattagga tcgattttaa gaaaccaaaa gaatgaggga agttaaaatg
     4381 cagacatctt taaaaacaga ctatgctatc cattctttaa tgctattggc gaaaacggga
     4441 aaagcgatta gtgttactga tttagcagag aaacagaata tatcaagaac atatttagct
     4501 aaagtgatgc agaaa
//
LOCUS       DATGCJ010000151         4862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_153619, whole genome shotgun sequence.
ACCESSION   DATGCJ010000151 DATGCJ010000000
VERSION     DATGCJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153619"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1006)
                     /locus_tag="VKN64_07595"
     CDS             complement(212..1006)
                     /locus_tag="VKN64_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMA60141.1"
                     /translation="MEKLIKKYQVLSFISFTYLFSWGLWTYLLITQEVNSITQWLIII
                     GGFGPLFGALMTNYFSGGKEKIINWFKTIINKNFNLKSIIFAVIYPLVVAFLVYMLIN
                     TFDFNHLIRKIDTRWYLYPLNLLTIMFFGGGQEEFGWRGFVLPNLLDKFNPFTASLIV
                     GGIWFIWHLPLIFIEGTAQAQLPIIWYLINIIALSIIFTFLRFIVNNRNIVPAVILHG
                     GVNISQNYFNIHAAKAYYLFTFINIIIALILIIKDQQFWFKKEFNK"
     gene            complement(1157..3832)
                     /locus_tag="VKN64_07600"
     CDS             complement(1157..3832)
                     /locus_tag="VKN64_07600"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMA60142.1"
                     /translation="MEIKEVYRYGHEKIIESLQTDPKKGLSSKEVEQRLNEYGPNTLK
                     KGSEVSPFEIFISQFKNILVILLIIAASISFFMGDTIEGIAVLAVIIINALFGFFTEY
                     RAEKSVEELKKMVTTQSKVLRDGKVISVDSDNVVPGDILIIEEGDRVTADGRLIEADN
                     LAVDESALTGESDPVNKKTDKIKEEEVPLAETNNMVYMGTAVTRGNGVAVITGTGPKT
                     EMGKISDLLSKTVDERTPLEEKLDQLGKTLIGITLLVAAIVSIFGIVSGRDVVDMVKT
                     GVALAIAAVPEGLPAVSTITLAIGMNKMARHKALVKSLPAVETLGSTTVICTDKTGTL
                     TENQMTVNKIYLDDREIDVTGTGYKPTGDFKEDNQTINIEDDKNLQLFLRAAALSSNA
                     SIDQSDDLWEVVGDPTEGALVTAARKANISKTNLEEDNYTRTEEIPFNSDDKFMAVSY
                     NTNKNKEYTYLKGAPDVVLKMCDKYKSGEQIHDLDQASKDKILEENHNLASQGLRMLA
                     VAYKEKSKDKLEDDIKENLIFIGLAGIMDPPRSDVKESILSAQKAGVRTIMITGDQSD
                     TAYAIGKQVGISKDDQKPITGSQLKKFSLEELEEKIKTHSIFARVSPKDKLDIIDALN
                     GNNEVTAMTGDGVNDAPALKKADIGVSMGQRGTAVAKEASDMVLLDDRFGTIVDAIKR
                     GRVIFDNIQKFIHYLFSCNLSEILFIFIGILLQLPTPLLALQILWLNLVTDVFPALVM
                     GFEQPEDNVMNKPPRDPEKAIMTNKFKLKIGIQGLIITMGPLFTYMYALNHFNTMESR
                     TIGFMTLAFVQLFHVFDVRRENGLGFDKTLLHNKYLWGAVVITIVLQLIAVYTPFLQR
                     VIHTVSLTLNMWYIVIIGSIIPVLAIQTYSYIKNR"
     gene            complement(3970..4617)
                     /locus_tag="VKN64_07605"
     CDS             complement(3970..4617)
                     /locus_tag="VKN64_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HMA60143.1"
                     /translation="MKQFIVVGLGRFGSSVARTLTDNGHDVLAIDQDHQIIQDISNSV
                     THAVEADATDEEALKTLGVRNFDVAIVSIGDNLHANILCTLILKELGVPYVIVKAQDN
                     LHGKVLTKVGADRVVYPERDMGERIAHNLISSNVLDYLEFAPDYSVVEIMASPNMVGK
                     SLAELEFRSRFDVNVMAIKRGQHLNMSPGASDKILENDTLIVMGKNENLDKVRDF"
     gene            complement(4648..>4862)
                     /locus_tag="VKN64_07610"
     CDS             complement(4648..>4862)
                     /locus_tag="VKN64_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMA60144.1"
                     /translation="FLDILFETVSAFGTVGLSTGVTPTLSKIGRALIIFTMFAGRVGP
                     LTFAVAFAERERKGIYHYPSEDVMVG"
BASE COUNT         1511 a          953 c          714 g         1684 t
ORIGIN      
        1 atattatttg catcagcaaa cttttgtgca ttttcctttg atctagaagc tacagccaat
       61 aggttgccgt tatcaacata attaaattca ttggcaaatg tatttgctat aactcccgtt
      121 ccaataattc cccaatttat agtcatcttt attccctcct ttttctttca cttataaaat
      181 actccaaatc ttgtttttat cctttttaat tttacttgtt aaattctttt ttaaaccaaa
      241 attgctgatc ttttattatt aggattaaag caataattat gttaataaag gtaaataagt
      301 aataagcttt ggcagcatgt atattaaaat aattttgtga tatatttact ccgccatgta
      361 agataactgc gggaactata ttcctattat tcacaataaa ccttaggaaa gtaaagatta
      421 tagaaagagc aatgatatta attaaatacc aaataatagg taattgtgct tgggccgttc
      481 cttcaataaa gattaaaggg agatgccaga taaaccaaat ccctcccact attagacttg
      541 cagtaaaagg gttaaattta tctaaaagat taggaagtac aaagcccctc cagccaaatt
      601 cttcctgccc tccgccgaaa aacataattg ttaataaatt cagaggatac agataccagc
      661 gggtatctat ttttctaatt agatggttga aatcaaaagt attaattaac atataaacta
      721 aaaaggcaac tactagagga taaataacag caaaaattat actttttaaa ttaaaatttt
      781 tatttattat tgtcttgaac caatttatta tcttttcttt acctcctgaa aaataattag
      841 tcattaaagc accaaataag gggccaaaac ccccaataat aatcagccat tgggttatac
      901 tattaacttc ctgagtaatt aataaataag tccagagccc ccaggaaaat agataagtaa
      961 aagaaataaa acttaaaacc tgatattttt ttattaattt ttccataata atacacctct
     1021 ctttttgact taattttatt ttaaaaaaga gagattaaat agttattatc taaatattaa
     1081 ttttttctta aatataaaaa gccttctaag tccttaaagg cccagaaggc tttcataatc
     1141 tcatatcttc tattttttat ctgtttttaa tataagaata agtttgaatt gctagaaccg
     1201 gaataataga gccgattata actatatacc acatattaag ggtaagactt acagtatgaa
     1261 ttactctctg taagaacggc gtataaacag ctatcagctg taatacgatc gttatcacta
     1321 ctgctcccca taaatattta ttgtgtagga gagttttatc aaagcccagt ccattttctc
     1381 ttcttacatc gaagacatga aagagctgga caaaggctaa agtcataaaa ccaattgttc
     1441 tactttccat agtattaaag tgatttaaag catacatata agtaaataat ggacccatcg
     1501 taataattag tccctgtatt cctatcttta gtttgaactt attggtcatg atagcttttt
     1561 ctggatctct aggtggtttg ttcattacat tatcttccgg ttgttcaaag cccattacta
     1621 aagctgggaa aacatcggtt accaagttca accagaggat ttgtaaagct aaaagaggtg
     1681 taggtaattg tagtaaaata ccaataaaga taaaaagtat ttcactgaga ttacaggaaa
     1741 aaagataatg aatgaacttt tgaatattat caaagattac cctacctctt ttaatagcat
     1801 ctacaatagt gccaaaacga tcatctaata aaaccatatc agaggcctct tttgctaccg
     1861 ctgtgcctct ctgccccatt gaaaccccta tatcagcctt tttaagagcg ggggcatcat
     1921 tgactccatc acctgtcatg gcagtaactt cattgtttcc atttagggcg tctatgatat
     1981 ccaatttatc tttaggagat acccttgcaa aaatggaatg ggtttttatc ttttcttcta
     2041 gttcttctaa actaaatttc tttagttgag atccagttat aggtttttga tcatctttag
     2101 atatgccaac ttgtttaccg atcgcatagg cagtatcact ctgatccccc gttatcatta
     2161 tagttcttac acccgctttt tgggcactca ggattgattc ttttacatca gaacggggtg
     2221 gatccatgat gcctgctaaa ccaataaaga ttaggttttc tttaatatcg tcttctaact
     2281 tgtctttact tttttcttta taggcaactg ccagcattct taagccctga ctggctaaat
     2341 tatggttttc ctctaagatc ttatctttac ttgcttgatc taaatcatgt atttgttcac
     2401 ctgatttgta tttatcacac attttcaata caacatcagg agcccctttt aagtaggtat
     2461 attctttatt tttattagta ttgtaagaaa cagccatgaa cttatcatca gaattaaaag
     2521 gaatctcctc tgttcttgta taattgtcct cttctaaatt tgttttggaa atattagctt
     2581 ttctagccgc tgtaactaaa gccccttcgg ttgggtctcc cacgacttcc catagatcat
     2641 cgctttgatc tattgaagca ttactgctta aggcagctgc tcttaaaaat aactgtaggt
     2701 tcttgtcatc ttctatatta atagtttgat tatcttcctt aaaatccccg gtcggcttat
     2761 atccggtccc tgtcacatca atttccctat catctaaata tatcttattt actgtcattt
     2821 gattttcagt aagggttccg gttttatcag tacagattac tgtggttgaa cccaaggttt
     2881 ctacggcggg taaactttta accaatgctt tatgtcgggc catcttattc attccgatag
     2941 ccaatgtaat agttgatact gctggtaaac cttccggtac agccgcaatt gctaaggcaa
     3001 ccccggtttt aaccatatca acaacatctc ttccactgac aattccaaaa atagatacaa
     3061 tagctgctac taagagggta ataccaatta gggttttacc aagctggtct aatttttctt
     3121 ctaagggagt tctttcatca accgttttac ttaacaggtc actaattttg cccatctctg
     3181 ttttaggacc ggtcccggtg attacagcta ccccattacc ccgggtaacg gcagttccca
     3241 tatagaccat attatttgtt tcagctaaag gtacctcttc ttcctttatt ttatctgttt
     3301 ttttattgac cggatcagat tctcccgtta aggccgattc atcaaccgct aaattatccg
     3361 cctcaattaa gcggccgtcg gcagtaaccc ggtctccttc ttcaataatt aggatatcgc
     3421 ctggtaccac attatcagag tctacactaa taactttacc gtctcttaat acctttgatt
     3481 gggtagttac catcttttta agttcttcta cggatttttc cgccctatat tcagtaaaaa
     3541 atccgaataa ggcattgatt attattacag ctaggacagc aataccttca attgtatctc
     3601 ccataaaaaa tgaaatactg gctgcaataa tgaggagaat cactaaaata ttcttaaact
     3661 gggaaatgaa aatttcaaag ggagatactt cagaaccttt ttttaaagta tttggtccat
     3721 actcatttaa tctttgttca acctcttttg agcttaaccc ctttttgggg tcagtctgca
     3781 agctttctat tattttttca tgtccgtatc tgtatacttc ctttatttcc attgaaattc
     3841 ccccaattaa gtaactatag tgaacatata tctttttaaa agacttttaa aaagattaat
     3901 taaaaactga tttaacagtt tttatagtag ataatattat ataaaaagaa aaaccaggct
     3961 atcctggttt taaaagtctc taactttatc taaattttca tttttaccca ttacgattag
     4021 ggtgtcgttt tctaatatct tatcactggc ccccggtgac atatttaaat gttgtcccct
     4081 tttaattgcc attacgttga catcaaagcg gctcctaaat tcaagttcag ccaatgattt
     4141 acctaccata ttgggtgagg ccatgatttc aactacactg taatctgggg caaattctaa
     4201 gtaatctaat acattggatg aaattaagtt atgagcaatt ctttcaccca tatctcgttc
     4261 gggataaaca acccggtctg ccccaacttt tgtcagaacc ttaccatgta gattatcctg
     4321 agctttaact atcacatagg gaactcctaa ttcctttaaa atcaaggtac ataatatgtt
     4381 agcatgtagg ttatctccta tactaacgat ggctacatcg aagtttctaa ctcccaatgt
     4441 ttttaaagct tcttcatcag ttgcatctgc ttctacagca tgggtaactg aattagaaat
     4501 atcctggata atctgatggt cctgatcaat tgctaatacg tcatgaccgt tatcagttag
     4561 ggttctagca acactagaac caaatctacc tagtcctact actataaact gcttcatctt
     4621 ttttacctcc taaatgaaca acttaaatta tccaaccatg acatcttcgg atggataatg
     4681 ataaatacct tttctttctc gttctgcaaa ggctacggca aaggttaatg gtcctaccct
     4741 accggcaaac atagtaaaga ttattaaagc tctccctatc ttacttaagg taggggtaac
     4801 tccggtcgat agaccaactg taccgaaggc agaaacggtt tcaaataata tatctaaaaa
     4861 gt
//
LOCUS       DATGCJ010000152         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_287665, whole genome shotgun sequence.
ACCESSION   DATGCJ010000152 DATGCJ010000000
VERSION     DATGCJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287665"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VKN64_07615"
     CDS             complement(<1..709)
                     /locus_tag="VKN64_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA60145.1"
                     /translation="MVEQNKLRKNIIFVILLALVLAFVLIPFITMISSSFKPNQEQNL
                     FPPRLLPQDPTTVHYEGVLNPEVFPFLTYFKNSLIVAFISAGIGVVIAIFGSYSFARL
                     KFTGRGAVQRGVLLVYMFGGVLLVIPLFQMITKLGLFDTRTSLIVTYIVQTLPVSLYM
                     LGNYFRTIPEEIEEAAIIDGCSRLEVIWRIVLPLSAPAIVSVFIYAFMIAWNEYLFAS
                     VFINSEALKTLPLGLNQL"
     gene            complement(703..1569)
                     /locus_tag="VKN64_07620"
     CDS             complement(703..1569)
                     /locus_tag="VKN64_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA60146.1"
                     /translation="MTGMEERDARLGVILILPTVILIAGLIFYPIGYNIFLSLHEVNM
                     NPNVANTFIGLEHFADILTDFDFWNSFQTSVIFTATTVIGTTLLGLGVALLLNREFPG
                     RPIVRGIILLPYVTPVISAVFGWKYLFDPVFGMANYFLVDMLHLLPQHINWIESPRYS
                     IYAVIIFNIWRNFPFAFLMILAELQSISKTLYEAAVVDGASHWQQFKYITLPELKFVL
                     GALIILRTIWNFYKFSEVYLLSSSVEVLPVYIYEKAFANYNFGEAAAISAILFVFVMG
                     FILFYVRKVLEW"
     gene            complement(1645..2964)
                     /locus_tag="VKN64_07625"
     CDS             complement(1645..2964)
                     /locus_tag="VKN64_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMA60147.1"
                     /translation="MKRKYLMLISLVLVFTLVFSFNAFAKTEIEFWTTQTEEERAQII
                     RDIARRFEEDNPNFTVNVVTVQENDIPTKLAAGMAANKLPEIVEMGAENILKLGAEGM
                     IDTKSVNEVIEVIGRDDFYDGALQMLKNPEGGQFGIPMHGWVQGIWYRTDLFEEKGLE
                     PPTSWENVLKAAEAFYNPDEQQYGIVIGTNKDSYARQTFTQYALSNNARIFDKEGNLV
                     FNSPEMVETLQYYSDLAQYTPPGPDGWRDARDLYLAGRVPMMMYSTYIMDDIGLERTG
                     YEGEKGSIVPNLVDKTALANKLTNKSSATFGQIVGLTLFKGNEEAKMRGAKLLAEYLM
                     SDQQYIDYLHMAPGGMNPTRRTIANDERYLDNEVIKVWGSKAADIAKAFDSIGKFGYV
                     EGRVFPKIGNITSQFIVGQTIMRMTENGWTAERAAEWGHEEMQDALD"
     gene            complement(3067..4068)
                     /locus_tag="VKN64_07630"
     CDS             complement(3067..4068)
                     /locus_tag="VKN64_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA60148.1"
                     /translation="MTVTLKDIAEKVGVSESTVSRVLNGIPKASKETREEIFRVAREL
                     NYTPNEVARSLVTKKTNTIGLIISDLSNLYFSMVADGIESVVAQNDYSLIISTTGGKE
                     AEELKYIRIFKEKRVDGLIYISGKMPRSCEQALQDLDIPVVVVSRHIHSDLPSVHIDN
                     VQESIKAVQYLIDLGHRNIAMISGDYKDIESGALRVKGYKKALENNNIEFNSDLVFEG
                     DFKIDSGIEAMKEILKLKTRPTAVFVSSDEMAVGAIKTIKKAGLKVPDDISIIGFDNN
                     IIARVSDPELTTVGQPESEIGETAMQMLHKLIQGEDVKEINVYLPCKLIKRESTAMV"
BASE COUNT         1311 a          880 c          707 g         1362 t
ORIGIN      
        1 aaagctgatt taaacctaat ggtagagtct tgagtgcttc actgttgata aagacagatg
       61 caaataagta ttcattccag gctatcatga aagcatagat gaaaaccgat acgatagctg
      121 gagcggagag cggtaaaact atccgccaga tcacttctaa cctggaacag ccatcaataa
      181 tggctgcttc ctctatttct tccggaattg ttctaaagta attacctaac atatagagcg
      241 aaaccggcag ggtctggact atatatgtta caataagtga ggtacgcgtg tcaaaaagac
      301 ctaacttagt aatcatttga aacaatggga taactaataa gacgccgccg aacatgtaaa
      361 ctaaaagcac acctctctgc actgctcccc ggccggtaaa tttaagccgg gcgaagctat
      421 aagaaccaaa gattgcaatt actaccccaa taccggctga aataaaggcg acaatcaaac
      481 tgtttttaaa ataggtaaga aaaggaaata cttctggatt taatacgcct tcataatgaa
      541 cagttgtggg atcctgaggc aacaacctcg gtggaaatag attctgctcc tgattaggct
      601 taaaagaaga tgagatcatt gtaataaaag gtattaaaac aaaagcaagt actaaagcca
      661 gtaaaatcac aaatattata ttcttgcgta gcttattttg ttctaccatt ctaacacctt
      721 ccttacatag aacaagataa agcccattac aaatacaaac aggatagcgg atattgcagc
      781 tgcttcaccg aagttgtaat ttgcaaaggc tttttcataa atataaacgg gcaatacctc
      841 aaccgaagat gataagagat aaacctccga aaacttgtag aaattccaga ttgttctcaa
      901 aataattaag gcccctaaaa caaacttcaa ttccggtaaa gtaatatatt taaactgctg
      961 ccagtggctg gcaccatcta ctaccgcagc ttcataaagg gtctttgata tagactgaag
     1021 ttccgccaag atcattaaaa aagcaaaagg aaagttccgc caaatattga atatgattac
     1081 cgcataaatt gaataacgcg gggattcaat ccagttaata tgttgaggca gtaaatgtag
     1141 catatctact aaaaaatagt tggccatacc aaatacaggg tcaaatagat atttccaacc
     1201 aaaaactgct gaaattacag gtgtaacata tggtaatagg attatccccc ttacaatagg
     1261 acggcccgga aactctctgt ttaaaagtag agctacccct aaacctagta gggttgtacc
     1321 tatcactgtt gtagctgtaa aaataacaga agtttgaaat gaattccaaa aatcaaaatc
     1381 tgttaaaata tctgcaaaat gttcgaggcc gataaatgta ttagctacat tcggattcat
     1441 attgacttca tggagactta aaaatatatt atatccaatg ggataaaaaa taagccctgc
     1501 tattaaaata acggtaggta atattaatat gactcctaac cgcgcatctc tttcctccat
     1561 tcctgtcatt ttttgtgcct cctctttaaa aagataaaaa gaaatactcc cgctaaaaac
     1621 agcgggagca ttatttgtat ttaattaatc aagtgcatcc tgcatttctt catggcccca
     1681 ttcggctgct ctttcagcag tccagccgtt ttcagtcatt ctcattatag tttgacctac
     1741 aataaattgg cttgtaatat taccaatttt ggggaatact cggccttcaa catatccaaa
     1801 cttacctatg ctgtcgaatg ctttagctat atcggcagct ttagaacccc ataccttgat
     1861 tacttcgtta tctagatatc tttcgtcatt tgcaatagtt cttctggtcg gattcattcc
     1921 accgggtgcc atgtggagat aatcgatata ctgttgatcg gacattagat attctgctag
     1981 taatttagcc cctctcattt tagcttcctc attaccttta aagagagtta gaccaactat
     2041 ttgcccaaat gtagcacttg atttattggt caacttatta gctaaggcgg tcttatctac
     2101 taagttaggc acaatagaac ctttttcacc ttcataaccg gttctttcca gtccaatgtc
     2161 atccatgata taagttgaat acatcatcat gggaacacga cctgctaaat aaaggtcgcg
     2221 agcatctctc caaccatcag gtccgggtgg agtgtactgt gctaaatcac tataatattg
     2281 gagtgtttca accatttcag gcgaattaaa gactagatta ccttctttat caaagatgcg
     2341 ggcattattt gatagtgcat actgagtaaa ggtttgacgg gcatatgagt ctttattagt
     2401 tcctatcact ataccatact gctgttcgtc tgggttatag aaggcttcgg ctgcttttaa
     2461 cacattttcc caacttgtcg gcggttcaag tcctttttct tcaaagagat ctgttctata
     2521 ccagattcct tgtacccaac catgcattgg gataccaaac tgtccacctt ccgggttttt
     2581 cagcatctgt agtgcaccat catagaagtc atcacggccg atcacttcaa ttacttcatt
     2641 aacagattta gtatcgatca ttccttctgc cccaagtttt agtatatttt ctgcacccat
     2701 ttcaactatt tcgggtaatt tattggcggc cataccggca gctaatttag ttggaatatc
     2761 attttcctgt acggttacta cgtttactgt gaaattagga ttatcttctt caaatcttct
     2821 cgcaatatcc cggattattt gagccctttc ttcttccgtc tgagttgtcc aaaattcaat
     2881 ttctgttttt gcaaaagcat tgaaactaaa caccaatgtg aataccaata ccaagcttat
     2941 caacattaaa tactttcttt tcaaagcttt tccctccttg tattgaattt tatgaacctc
     3001 ttgtttattt aaacccttga gcagggtata aacctatttg ggcaatcgtt tgactaaata
     3061 aaatttttac accatagccg ttgattcccg ctttattaat ttacagggca agtagacatt
     3121 aatttctttc acatcttcac cctgaattaa tttatgcagc atctgcatgg cagtctctcc
     3181 gatctcactt tcgggttgcc cgactgtagt tagctctgga tcagatactc tagctataat
     3241 attattatca aaaccaatga ttgatatatc atcaggaact tttaggcctg cctttttgat
     3301 tgtttttatg gcaccaacag ccatttcatc actgctgaca aagacagccg tcggcctggt
     3361 ctttaacttg agtatctcct tcatagcttc aataccactg tctattttaa aatctccttc
     3421 aaaaacaagg tcggagttaa attcaatatt attattttct aatgcctttt tatatccttt
     3481 aactcttaag gcacccgatt caatatcttt atagtctcca ctgatcatgg cgatatttct
     3541 atgacctaaa tcaattaaat attgaactgc ttttatggat tcttgcacat tatcgatgtg
     3601 aactgaaggt aagtcagaat ggatgtgtct tgatacaact actactggaa tatcaagatc
     3661 ttgaagggct tgttcacaac tccggggcat ctttccgctg atatatataa gtccatctac
     3721 tcttttctcc ttgaaaattc gaatatattt gagttcttct gcttctttac ctcctgtagt
     3781 ggagataatc aaactgtaat cattttgggc tacaacgctt tcaataccat cagcaaccat
     3841 agaaaagtag agatttgaga ggtcactgat gattaatcca atcgtatttg tcttcttggt
     3901 aacaagagat ctggctactt cattaggagt atagtttagt tcacgagcta ctctgaatat
     3961 ttcttcacgt gtttccttac tggctttcgg tataccattt aatacacgag agactgtcga
     4021 ttcagataca cctactttct ccgcaatatc tttcaaagta accgtcatat aaattctcct
     4081 cttcttttgg tcaaacgttt gacctttcta tttattatta tataaaaaaa tcttataatg
     4141 tcaagcctaa tttcagataa ttatgctaat agttttaaac attcaaaaag ggcctgtgtt
     4201 caaacaggcc cctctttaga gtatctctta atttttaaga ctagaactta taatgagttc
//
LOCUS       DATGCJ010000153        15738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_110976, whole genome shotgun sequence.
ACCESSION   DATGCJ010000153 DATGCJ010000000
VERSION     DATGCJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110976"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VKN64_07635"
     CDS             complement(<1..216)
                     /locus_tag="VKN64_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin1244/Lin1753 domain-containing protein"
                     /protein_id="HMA60149.1"
                     /translation="MGKDAYYFSHDYNARQDPKILALKADYGVEGYGRFWVIVEMMAE
                     QDDYKLKLNQITNNALAVEFMCQKDEAE"
     gene            complement(274..735)
                     /locus_tag="VKN64_07640"
     CDS             complement(274..735)
                     /locus_tag="VKN64_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60150.1"
                     /translation="MNKVKPGQDKEHLRKEYRDIIFKSGYYEDLSYDAQMIYIYLKDL
                     FIENYEQDYPEQITDGFVKFKDFIEEYGEEMFDAFNVEGNYEETRFSMLNAIKELEDN
                     KLITVVLLKDADNKPIILLYLQDINLSYKEKVLDSMLKEMFYNEYKKRKQH"
     gene            complement(779..1018)
                     /locus_tag="VKN64_07645"
     CDS             complement(779..1018)
                     /locus_tag="VKN64_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA60151.1"
                     /translation="METKNDIFENLIRETSNKYPISLKVRDIIELTGYSQATVYRMLE
                     CEEIPGAKKIRGWRVPRDVFLAWWMADGIGDNEII"
     gene            1192..1974
                     /locus_tag="VKN64_07650"
     CDS             1192..1974
                     /locus_tag="VKN64_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60152.1"
                     /translation="MIGQKIKRLRKEFNITQKELGEELGVTTSMIGMYETNARKPSYE
                     ILEKLSNFFQASTDYLMSDIPFKNKIEAQHSISIAVVKEIKENLDYYLEILEPYEGID
                     LLKSKGIQEEVITYCDSIIDKFYKNDDLSYIELFELSNLIFKSIKWSPDYDGKIYLED
                     YMAITLLLELNLNEIEKITLDLSESYLLKITKSDHFYLEQSPNLLKTSTSMTEYTKNE
                     ENQNTDIDSKYQSIINILKDKNLNEEEIEELKNYINYLLSKR"
     gene            2036..3202
                     /locus_tag="VKN64_07655"
     CDS             2036..3202
                     /locus_tag="VKN64_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMA60153.1"
                     /translation="MAHIKKLYDQDNKIKGYKAFVEVGKDPATGKRKRQTKVFKRKKD
                     ADKWLINKVNSIQNGSYVNPSQITLGDFILNRWLENHKKPKVTITTYDRYKSLINAHI
                     IPALGEIPIQELQPFHIEHYFSQKRKKGIRNKKGLSENSLKRHYVLLNNALKRAVKLK
                     LIKYNPMQAIESPKPEKYEAPVMKENEYNKLLESLKEDTFMYTFVMTLISTGMRRSEI
                     LGLEWNDIDLKQGTIFIRKRLITITGGIKHEHKTKNESSKRLIKIPKALIPIIKSYKI
                     KQKENKLFFGTDYYKEKDYVFCKPDGYPYNPQTLNVKFNIVLRKINLPQKFTLHTLRH
                     TFATINLRNGVPAKVVQEMLGHSTITTTLDLYTHVDLDMQEEAAEKLQNTIKFE"
     gene            complement(3734..4615)
                     /locus_tag="VKN64_07660"
     CDS             complement(3734..4615)
                     /locus_tag="VKN64_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60154.1"
                     /translation="MKLEKFLESYIKAQNKKINKILKETKQQTLSQFNDLFKELYEDL
                     KQFESTHERFSMIMSKLGWPPQSNLNVNEVSEIVYYYDEYGEEATKKEIDKYFLERYT
                     ADKIDEKVKNWDKKKIMNKRMPIIKEVIKAHKNGDYYLSVATLLPQIEGIIADGFGHV
                     GQMWKDDLKEYITRLNTNRRIFNNLLKDFYLKIILVGFGHGEEINSCLSRHAIVHGGD
                     VDFGTKINSLKTILLLNYVQDCFNIILIKEEDVYHEYGCDILMSKKDKKWCPYNSPVS
                     ITYKDKTPCHNCNPNSK"
     gene            complement(4677..8114)
                     /locus_tag="VKN64_07665"
     CDS             complement(4677..8114)
                     /locus_tag="VKN64_07665"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HMA60155.1"
                     /translation="MPGLFNKKILEQRIKNYSVDNIERKVNQIKNWQKNLADIKGLNE
                     VELQNAFMGAIFEDILGYEDAPQKDKSTLKSQASTDVDATRPDGIIGFYDKTKRKEED
                     HRAVIELKGPQIPLEKDQKRKGTTYRSPVDQGFSYTNKLDKCRWVIVSNFTEIRLYQV
                     GRSKEYYEIFYLNELDDIEEFKRFHYLLSKNNLISKDGKSQTLQLTEKTQKRHEDISV
                     EFYNLYKNARINLFGHLKENNENIDFEVLLEKAQKFLDRIIFICFCEDKGLLPNDLLH
                     NAIKQAKNSFYSGDTRIWDQIRGVFKAIDKGMPNKNINAYNGGLFEFDKVLDVLVIKD
                     DFFDVIYDFSDYDFDSDVDVNILGHIFEQSISDIEKLKSDIKDNEFDKNKSRRKKEGI
                     YYTPQYITSYIVENAVGGYLEDIKEDMGYYDLPDIEEAGSDSWQTRYTNQHLEFYDNY
                     EEKLKEIKVLDPACGSGAFLNQAFDYLLQEHQWLNKQRDLLKGGQSSIYAVEALQRTI
                     LKNNLYGVDLNEESVEITKLSLWLKTANKNKQLTNLDNNIKCGNSLIDNPEVVGEKAF
                     KWEKEFADIMVDGGFDVVIGNPPYVRQEKIKNIKPYLSDNYKVYNGVADLYVYFIEKS
                     IKLLDNQGYFSFIVSNKFTKAKYGEKLRKFLMNYTIKQINDYSGEKVFKDAAVDPCIV
                     VLKKKISNLSNKVELNNNVLLTQDKIAQYSWTFSNLDGIKIYEKVKDKGINLEHWDLD
                     IYFGIKTGLNKAFIISQNKKDEIISKNPNAKKIIRPLITGQDISRYRYSFNNQWLILI
                     ENGWTDKNRGNTEPYKFVKNKYPFVIKHLKNIGDKIERGEVYSKGRGLYKRDDQGDYW
                     WELRPCNYYDYFEKPKIIYKDISERLAFAYDDGNIYFNNTVYFLNVGNFYLLSVLNSK
                     LINFYYKNISSNLGLNASRGFTIFIKKIPIPKNIVNNRNDLAKKTKLITNKYKKYSKK
                     KLIDFNGYIKLYCSSNKNSIKNILEKNEFANKIYSGRARKVRNFTVNINTNIVTIYSE
                     KSSSGKYELLKYEENNEYKRRYIKYYLENLTEDQLEKTDSFSGNLLEKVLQIEIPDYN
                     KDQVVRKVVNEWENLQQEIKGLENEIEKTDNEIDQMVYDLYNLTEEEINIIENNMNK"
     gene            complement(8213..8515)
                     /locus_tag="VKN64_07670"
     CDS             complement(8213..8515)
                     /locus_tag="VKN64_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60156.1"
                     /translation="MIFSQSVMGGNGLDEVDYENIEVDKEGNLVLTKDQYVKLANYIE
                     ELKAENLKLNMKLEQANKELEKAYADENPFNFTRLSDKITGAGLAALIILIVLNIN"
     gene            complement(8484..8741)
                     /locus_tag="VKN64_07675"
     CDS             complement(8484..8741)
                     /locus_tag="VKN64_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60157.1"
                     /translation="MVNKMWIYTAVLVILLILGYWLGSEFAALIGVVLVFLRLRKSNK
                     DKLKAAQNNIDKAGEDVEAKKHDANSALKFFDDFFSKRNGR"
     gene            complement(8746..8946)
                     /locus_tag="VKN64_07680"
     CDS             complement(8746..8946)
                     /locus_tag="VKN64_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60158.1"
                     /translation="MKDLKEDYTDFTKSDKFQWFMTILITLLILRILNYIPEPDFVEL
                     IKWSIFGLFGSEGLDQFTNIRK"
     gene            complement(8951..9481)
                     /locus_tag="VKN64_07685"
     CDS             complement(8951..9481)
                     /locus_tag="VKN64_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HMA60159.1"
                     /translation="MTDIYDVELFENIFNHIIDLEGGYSNHRYDLGGETKFGITEATA
                     RRNGYEGSMYDLSLQTAKGIYYQEYWLEPGFNKIKEYTIACELFEQGVNLGPHEAVKN
                     FQKSINYACRNVLEVDGIIGAKTLTTYKKCSNKNEIYVLLNVFQAQKYISIVKNNPSQ
                     RAFLKGWLKRVNLTKE"
     gene            complement(9486..9833)
                     /locus_tag="VKN64_07690"
     CDS             complement(9486..9833)
                     /locus_tag="VKN64_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60160.1"
                     /translation="MEKHEKDTCPFKKEIEEIIKKTGQKEWYENKDLYDLIVRLTAQT
                     EVLSNKLDNTIRIVGKYNGLREDLLSIKKRIDYIETEKLAKNKIGDQIIKWSGWIIAI
                     ISLGFTAVKIFGW"
     gene            complement(9849..10190)
                     /locus_tag="VKN64_07695"
     CDS             complement(9849..10190)
                     /locus_tag="VKN64_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60161.1"
                     /translation="MAYKNQDFEVWQGDDFKLNYNVDGVQAAEDLTAAKWAISRSRSG
                     AAMLTKDSNITGEIAIDTVSNIVSVILFSADTLNLKEQKYYCELEVFDSENLRTTIAI
                     GTMDLQPTILK"
     gene            complement(10191..10664)
                     /locus_tag="VKN64_07700"
     CDS             complement(10191..10664)
                     /locus_tag="VKN64_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60162.1"
                     /translation="MSAYFILALDTQGPAAEIVSPGFVDMEEEFTITVNSDETLKAAY
                     IHELYLIDSLGTRFDLSYSLNTHSLVSTLSLNSLGASSGSLTIYALIYDEVLNPSEVV
                     SKELSTEIPLIINIVSLEAEIKKELSLNASLEKEKDLNSSIIKTISLQGRIGGDQ"
     gene            complement(10688..11368)
                     /locus_tag="VKN64_07705"
     CDS             complement(10688..11368)
                     /locus_tag="VKN64_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60163.1"
                     /translation="MANYFLLTLDTTGPQSPAISLESGAQYATQQLINADISTADGET
                     TGYQMKIWGDVDLTENPNIQDTEVNSSWITYSATQQVKLSIGDGAKTVYLKIRDDVLN
                     ESAQTSDSITLDTSLPVVTISGPDVNKISKIATANVSSFSFTVNEIFDEYKVKVVAST
                     GAAHDTGTLIGTTNGSTNMSGVAGDYPAETAINCQVYGSDLEIASAGDGEKIVKVFAK
                     DKSGAWSV"
     gene            complement(11387..11857)
                     /locus_tag="VKN64_07710"
     CDS             complement(11387..11857)
                     /locus_tag="VKN64_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMA60164.1"
                     /translation="MLKLYLNLKYKFIKGDKEASEKMSKMLIEEENLKDHFDVGRFVN
                     KKMTYKSDKLFGIIDTIQDLDYTVKSGYKGDCDDFSMLAYRLLEQLGYNPYLLTIIPL
                     KFWKGHVVCLYKVKGYYYFISTNGNTAIGGFQQIRGIFDYLKYKYALTFSIDKI"
     gene            complement(11877..12425)
                     /locus_tag="VKN64_07715"
     CDS             complement(11877..12425)
                     /locus_tag="VKN64_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60165.1"
                     /translation="MSIQRRNSNGVLQEENQDDSTYYISQGTGVYKMGIYNCTWTAGD
                     AGDILVVRFSYKNVDEHSSNSMTFETGTIDAEVITPISIITSNLPTINIVGYTKDKIS
                     DEVGYQTSTVTFQSDQDLVEWEARADGTGHGSGLLVGSGGSVTANTDVTFDVDYTELT
                     QGDKTYQINVYGKNSDGLWSDS"
     gene            complement(12677..13105)
                     /locus_tag="VKN64_07720"
     CDS             complement(12677..13105)
                     /locus_tag="VKN64_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60166.1"
                     /translation="MRKADQKLLDYIKQPLVKHRSKMKDIKIEGYKLYYGDGFVFTAK
                     GSYLHLFKEWHRAPFENIQIIMLYENKYFGADKKHYRMSISGFDYYLFDGIDFMACND
                     TRKFSLIPKNSLKYGWYMDKEKFKEINEKAIMDFWLKGES"
     gene            complement(13130..15436)
                     /locus_tag="VKN64_07725"
     CDS             complement(13130..15436)
                     /locus_tag="VKN64_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60167.1"
                     /translation="MAYSQFTLFEEYQPYERPYNDFAVWNPSQNMAQAIDEYMGVRGH
                     SIMAELGYTDSPPVRWIKAGSVYNTTLVCKFESPIELGDEQAAEHGIPAGTPCIKTGQ
                     EEIMLRAGQNYSNATDYLYFKVIYDWSTLSIHLMQYTSSTNSHTSINSVSTPNVIEYA
                     GDMLQYYLVKIELIDDTINVKFWQSIDPEPASPTLTATWSGTNSGHIILRQYLKAQFD
                     GYETVNTTNMIFMHSDASIDTEQYRSALIQGAITHPSGSPVNWSGLQVIGFDKWERFP
                     HIDAPDNTEIAANGGDVSTDGNGTYSFYLEAVEHYISPWPRNVGYDPNEQIITPQGGQ
                     THTVNIQYISLSNNVNTLDRLWQIRYYPSYTFYLSVDLDLTDYNFRHRADHLADYGNW
                     IPASWTTAQFVGYDTATGSYANRTIYNAELNQITNKSLAIPIRERNYMSNAFFRNSGR
                     SDQTEGPTLYKIDFKNITVTSNLPYIALVFSYVTCSSVQYCNVQGTINAELYYNGSPV
                     AECVGGMAGLEAGSAGASTTHSFNEVEVTINGPGIIKYVGGYIGQSYLGQVNRTFTKG
                     SITAASANYVGGFVGNHYNDNYYNYSRCSVVGNNYVGGYAGYNHSFIADAYSTGSVNG
                     SLNVGGFCGYNSGIIQKVYYDLQTAGQSDTGKGVPRTTEEMTYPFDLGSTYIDWDMGT
                     MPPANSYNYPPGVDPETDSVEFVIYEVNDGYPALKAVDGYWWPEPETGVFWVKKSGIW
                     HSAEAYVKSGGNWYLVLLSAVKTDGIWN"
     gene            complement(15426..15575)
                     /locus_tag="VKN64_07730"
     CDS             complement(15426..15575)
                     /locus_tag="VKN64_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60168.1"
                     /translation="MPADKTILINGNKRIKSDLFKLDKVKINKAEIESLPEISVKEKV
                     KDNGL"
BASE COUNT         5117 a         3052 c         2027 g         5542 t
ORIGIN      
        1 ctcggcttca tctttttgac acataaactc tacggctaaa gcattattag ttatttggtt
       61 tagcttgagc ttataatcat cctgctcggc catcatctct actatcaccc agaaccgtcc
      121 gtatccctcc accccataat cggcctttaa agctaaaatc ttaggatcct gccttgcatt
      181 gtaatcatga gaaaaataat aagcatcttt acccatctat tcaccccgtt aatatgagat
      241 accccagccc tgcagggctg ggtcctatca gtattaatgt tgttttcttt ttttgtattc
      301 attataaaac atctctttaa gcatagaatc taataccttc tctttgtaag atagatttat
      361 atcttgaaga tataataaaa ttattggctt attgtcagca tccttaagaa gcacaactgt
      421 tattaactta ttgtcttcta attctttgat agcatttaac atactaaacc tggtctcctc
      481 ataattaccc tcaacattaa aggcatcaaa catttcttcc ccatattctt ctatgaagtc
      541 tttaaatttt acaaaaccat ctgtaatttg ttccggataa tcttgctcat aattttcaat
      601 aaacaaatct tttaagtaaa tgtatatcat ctgggcatca tatgaaaggt cttcataata
      661 gccgctttta aatattatat ctctgtactc ctttcttaga tgctctttat cttgacccgg
      721 tttgactttg ttcatttaca tcttcctttc ctaaaggttt atttcatact tagacagact
      781 aaataatctc attgtcacca attccatccg ccatccacca ggctaaaaac acatcccgag
      841 gtacacgcca gcctcttatc tttttagcac cagggatctc ttcacattcc agcatcctat
      901 aaactgttgc ctgactatat ccggttagtt cgattatatc tcttactttt aaagatattg
      961 gatatttatt actagtttcc ctgattaggt tttcgaaaat gtcattcttt gtttccattc
     1021 ttatccctcc tttttttatt tatattaata acattatata actttaagtc tgaccttgtc
     1081 aacttttttc tcactttaag tatgctttac agtaaaatat tttgttttaa gttgactttt
     1141 tcataacttt aagttataat aaaactaata gttataaagg agggctaaac agtgatcggc
     1201 caaaaaataa aaagactacg aaaagaattc aatataacac aaaaggaact tggagaagaa
     1261 cttggagtta ctacttcaat gattggaatg tatgaaacca atgcaagaaa acccagttat
     1321 gaaatattag aaaaactatc aaattttttt caagctagta cagattattt aatgagtgat
     1381 attcctttta aaaataaaat agaagcacaa cattctatta gtatagcagt agttaaggaa
     1441 atcaaagaaa atcttgatta ctacttagaa attttagaac cttatgaagg aattgatttg
     1501 ttaaaaagca aaggaatcca agaagaagtg attacttatt gtgatagtat aatagataaa
     1561 ttttataaaa atgatgactt aagttatatt gaattatttg aattatctaa tttaattttt
     1621 aaaagtatca agtggtctcc tgactatgat ggtaaaatat atctggaaga ttatatggcc
     1681 ataaccttat tattagaatt aaatttgaat gaaatcgaaa aaataacttt agatttgtcg
     1741 gaaagttatt tgcttaaaat tacaaaatct gatcattttt acttagaaca aagccccaat
     1801 ttattaaaaa catccacatc aatgacagaa tataccaaaa atgaagaaaa tcaaaataca
     1861 gatatagatt caaagtatca atctataatt aatatattaa aagacaaaaa tttaaatgaa
     1921 gaagaaattg aagaactcaa aaactatatt aattatctgc tatctaaaag ataacacaac
     1981 aatcatatta acttctttaa aataaaactt tattatattg attggaggtg aagaaatggc
     2041 tcatataaaa aaattgtatg accaggataa taaaattaaa ggttataaag cgtttgtaga
     2101 ggtaggtaaa gaccccgcga caggtaaaag aaaaagacaa actaaagtat ttaaaagaaa
     2161 aaaagatgct gataaatggc ttattaataa agtcaattcc atacaaaatg ggtcttatgt
     2221 aaacccatcc caaattactt taggggactt tatattaaac agatggttag aaaaccataa
     2281 aaaaccaaag gttactatca ctacttatga tagatataaa tctctaataa atgctcacat
     2341 aatccctgca ttaggggaaa ttccaatcca agaattacag cctttccata ttgaacatta
     2401 tttttcgcaa aaaagaaaaa agggaataag gaataaaaaa ggattatcag aaaactcact
     2461 aaaaagacat tatgtcttat taaataatgc cctcaaaaga gctgtcaaac tcaaattaat
     2521 aaaatataac ccaatgcaag caatagaatc acctaagcca gagaaatacg aagctccagt
     2581 tatgaaagaa aatgaataca acaaattgtt agaatcgtta aaggaagata cctttatgta
     2641 tacatttgtt atgacattaa tttcaacagg gatgagaaga agcgagatat taggcttgga
     2701 atggaatgat atagacttaa aacaaggaac tatttttatt agaaaaagat taattacgat
     2761 tactggcggg ataaaacatg aacataaaac aaagaatgaa tcaagcaaaa gacttatcaa
     2821 aataccaaag gcactaatcc ctataattaa aagttataaa attaagcaaa aagaaaacaa
     2881 attatttttc ggcactgatt attataagga aaaagattat gtcttctgta aaccagacgg
     2941 ttatccttat aatccacaaa ctttaaatgt gaagtttaat attgtattaa gaaaaataaa
     3001 tttgccccaa aaatttactc tacatacttt aagacacaca ttcgctacaa ttaatttaag
     3061 aaatggcgtt ccagccaaag tagttcaaga aatgctaggg cactcaacaa ttacaacaac
     3121 tcttgactta tatacacacg ttgatttaga tatgcaagaa gaagcagctg aaaaattaca
     3181 gaatacaata aaatttgaat aaaaaaaata agcgcctatc taaggcactt ataagtttga
     3241 caaatattca tttattatct aaataatttt ctcggcacct tttgggcacc ttgggtgctt
     3301 ttttcttgct ttctataaaa tcaacgaaac ctcatggttc cagtcatatc aatggcggag
     3361 agacggggac tcgaaccccg acacctttcg gcttcaccgg atttcaagaa agtcttgagc
     3421 atatttttac attaaatata gcacataatg tttaatatag cagcactttc ttgggacacc
     3481 ataaatcaat attaaacata aataaaagga aataaagatg tatgggaaca ttatgggaac
     3541 acaaaactta tttattcaac caaaatcagc tgtgttccca actttgttcc atacataaat
     3601 ttacaattct atatgttctc attcacttgt ttctttcaat ttattataca ttcttttaac
     3661 ttgcttttta tatcctaaga tttatttatt atccaatcaa acttctaaac caaatacaaa
     3721 aataaatata taactactta ctattaggat tacaattatg acaaggggtt ttgtctttat
     3781 atgttattga aactggagaa ttataggggc accatttttt atcttttttc gacatcaata
     3841 tatcacaacc atattcatga taaacatctt cttcttttat aagaattata ttaaaacaat
     3901 cttgcacata atttaaaagt aaaatagttt ttaaagaatt aattttagta ccaaaatcta
     3961 catcaccgcc atgaactatt gcatgtcggc ttaaacagga attaatctcc tcaccatgtc
     4021 caaagccaac taaaattatt tttaaataaa aatcttttaa aagattatta aagattcttc
     4081 tatttgtatt caacctcgta atatattcct ttaaatcatc cttccacatt tgccccacat
     4141 gaccaaatcc atctgctatt ataccctcta tttgaggcaa taaagtagca acagacaaat
     4201 agtaatctcc attcttgtga gcttttataa cttcttttat aattggcatt cttttattca
     4261 taattttctt tttatcccaa ttctttactt tttcatctat cttgtcagct gtatatcgtt
     4321 ctaaaaagta tttatcaatt tcttttttag tagcctcttc cccatattca tcataatagt
     4381 agactatttc agagacttca ttcacattaa gattggattg tggtggccat cctaattttg
     4441 acataatcat tgaaaatctt tcatgagtgc tttcaaattg ctttaaatct tcatacagtt
     4501 ctttaaataa atcattaaat tgtgacaaag tttgttgttt tgtttccttt aatatcttat
     4561 taattttttt attttgggct tttatataag attctaaaaa cttttctaat ttcatttctt
     4621 aaaaaccctc tttctaaaaa attaataatt gtctttattt aaaatacaac cttgtattat
     4681 ttattcatat tattttcaat aatattaatt tcttcttctg ttaaattata taaatcatat
     4741 accatttgat ctatttcatt atcagttttt tctatctcat tttccaaacc tttaatttcc
     4801 tgctgtaagt tttcccattc attaactact ttcctaacta cctggtcttt attataatct
     4861 ggtatttcta tctgtaaaac cttttcaagt aagttgccgc tgaaactatc tgttttttct
     4921 aattgatctt cagttaagtt ttcgagatag tattttatat atcttctttt atattcatta
     4981 ttttcttcat attttaatag ttcatattta ccactgcttg atttttcaga ataaatggtt
     5041 actatattag tattaatatt tactgtaaaa tttcttactt ttctggctct cccactatat
     5101 attttgtttg caaattcatt tttttcaaga atatttttaa ttgaattttt attcgatgaa
     5161 caatatagtt taatgtatcc attaaaatct ataagttttt tctttgagta tttcttatat
     5221 ttatttgtaa taagctttgt tttttttgcc aaatcatttc tattattcac tatattcttt
     5281 ggaataggta tttttttaat aaatattgta aaacctcttg atgcatttaa tcctaaattt
     5341 gaagatatat ttttataata aaaattaata agcttagaat ttaataccga taataaataa
     5401 aagttgccta catttaaaaa atatactgta ttattaaaat aaatattccc atcatcataa
     5461 gcaaatgcta atctttcgga aatatcttta tatataattt taggtttttc aaaataatca
     5521 taataattac acggtcttaa ctcccaccag taatcacctt gatcatccct tttatataat
     5581 cctcttcctt ttgaatatac ttcccccctt tcaattttat ctcctatatt ttttaaatgt
     5641 tttataacaa agggatattt attttttaca aatttataag gttctgtatt acccctgttt
     5701 ttatctgtcc aaccattttc tattaaaatt aaccactgat tattaaaaga atatctatat
     5761 ctggaaatat cttgtccagt aattaatggt cttataattt ttttagcatt tggatttttt
     5821 gatattattt cgtccttttt attctgactt ataataaaag ctttattaag tccagttttt
     5881 attccgaaat atatatcaag atcccaatgc tcaagattta ttcctttatc ttttactttt
     5941 tcataaattt ttattccatc taaattcgaa aaagtccaac tatattgggc tattttatct
     6001 tgagttaata aaacattatt atttaactct actttgtttg ataaattcga aattttcttt
     6061 tttaaaacaa ctatacaagg atcaactgct gcatctttaa atactttttc ccctgaataa
     6121 tcattaattt gtttaattgt ataattcatt aaaaattttc ttaacttttc cccgtattta
     6181 gctttagtga atttgttaga tacaataaat gaaaaataac cttggttgtc taaaagttta
     6241 atgctttttt caataaaata tacatatagg tctgctactc cattataaac tttataatta
     6301 tcacttaaat atggtttgat attttttatt ttttcttgcc gtacataagg aggatttcca
     6361 atgactacat cgaaaccacc atcaaccata atatcagcaa actctttttc ccatttaaaa
     6421 gctttctccc ctacaacttc tggattatca attaaagaat taccgcattt aatattatta
     6481 tcaaggttag taagttgttt atttttatta gctgttttta accataatga tagttttgta
     6541 atttccactg attcttcgtt tagatcaacc ccatatagat tatttttcaa aatagttctc
     6601 tgtaatgctt ctacagcata aatgctgctc tggccgcctt tgagtaaatc tctttgtttg
     6661 tttaaccatt gatgctcctg caacaaataa tcaaaagcct gatttaaaaa agccccactt
     6721 ccacaggctg gatctaaaac cttaatttct tttaattttt cttcataatt atcataaaat
     6781 tcaagatgtt gattagtata tctagtctgc catgaatcac tgccagcttc ttctatatca
     6841 ggcaaatcat aatatcccat atcctcttta atgtcttcta aataaccgcc aacagcgttt
     6901 tccactatgt agctggttat atattgaggt gtataatata taccttcttt tttccttcta
     6961 gatttatttt tatcaaattc attatccttt atatctgatt tcaatttttc tatatcactt
     7021 attgattgtt caaagatatg tcccaaaata ttgacatcta catcactatc aaaatcataa
     7081 tcggaaaaat catagataac atcaaagaaa tcatctttta ttacaagaac atctaaaacc
     7141 ttatcaaatt cgaacaaacc accattgtag gcattaatat ttttgttagg catacccttg
     7201 tctatggctt taaatactcc tctaatctgg tcccatatac gtgtatcccc ggaataaaaa
     7261 gaattcttgg cctgttttat agcattatgt aataaatcat tcggcagcaa tcccttgtct
     7321 tcacagaaac agataaaaat aatcctatcc aaaaactttt gggctttttc caataatact
     7381 tcaaaatcta tattctcatt gttttctttg aggtgtccaa aaagatttat tctggcattt
     7441 ttatataaat tataaaactc tacagatata tcttcgtgtc ttttttgagt tttttcagtc
     7501 agctgtaatg tctggctttt tccatctttg gaaattaaat tatttttaga taataaataa
     7561 tgaaaccttt taaattcctc tatatcatct agttcattta aataaaatat ctcataatac
     7621 tctttacttc ttcctacctg atacaaccta atctctgtaa agttagaaac tataacccac
     7681 ctgcatttat ccagcttatt agtatagcta aaaccctggt ctacaggtga tctataggta
     7741 gttccttttc ttttttggtc tttttcaagg ggtatttgtg gcccttttaa ttcaattaca
     7801 gctctatggt cctcttcctt tcgctttgtt ttatcataaa atccgattat accgtcaggc
     7861 cttgttgcat ctacatctgt tgaagcctgt gattttaaag tgctcttatc tttttgtggt
     7921 gcatcttcat agcctaaaat atcttcaaat atagctccca taaaagcatt ttgtaattca
     7981 acttcattta accctttaat gtctgctaaa ttcttttgcc aattttttat ttgattaact
     8041 tttctttcga tattatcaac agaataattt tttatccttt gctctagtat ttttttgtta
     8101 aataagcctg gcatatttta acctccaaaa ttctaaatat cttattaaga ttagttcttt
     8161 gaaggataaa ataatcctgc ataaatagtt agacattata taacaataat attcaattta
     8221 tattcagcac aatcaaaata atcaaagctg ccaacccggc ccctgttatt ttatctgaca
     8281 accgagtaaa gttaaaagga ttttcatcag cataggcttt ctctaattct ttattcgcct
     8341 gttctaattt catatttagt tttaaatttt cagcttttaa ttcttcaata taatttgcca
     8401 acttaacata ttgatccttc gttaggacca ggttcccttc tttatcaact tcaatgtttt
     8461 catagtcaac ctcatctaga ccattacctc ccattacgct ttgagaaaaa atcatcaaaa
     8521 aacttaagag cagaattagc atcgtgcttc ttcgcttcaa catcctcacc tgccttatct
     8581 atattattct gggcagcctt tagtttgtct ttgtttgatt ttcttaacct tagaaaaact
     8641 aaaaccaccc ctattaaagc tgcaaactca gatcccagcc aataacctaa tattaataaa
     8701 ataaccaaca ctgcagtata tatccacatt ttattaacca ttaaatcact tccttatatt
     8761 agtaaactga tccaggccct cagatccaaa gaggccaaaa atagaccact tgattaattc
     8821 cacaaaatcc ggttctggaa tataatttaa gatccttaaa atcaataaag taataagtat
     8881 ggtcataaac cactggaact tatcactttt tgtaaagtct gtgtaatcct cttttagatc
     8941 tttcaagtaa tcactccttc gttagattaa cccttttcaa ccagcctttc aaaaaggccc
     9001 tttgggaagg attgttttta acaatagata tatacttctg agcctgaaat acgttcaata
     9061 aaacgtaaat ttcgttctta ttagaacatt ttttataagt agttaaagtc tttgctccaa
     9121 taattccatc tacctctaaa acattccgac aggcataatt tatgcttttt tgaaagtttt
     9181 taaccgcctc atgcggtccc aggttaactc cctgttcaaa aagttcacag gcaatagtat
     9241 attcctttat tttattaaac ccaggctcca gccaatactc ttgataataa atcccttttg
     9301 cagtctgtaa agacaaatca tacatagagc cttcataacc attccgcctg gcagttgcct
     9361 ctgtgattcc gaattttgtc tcaccgccca gatcatatct gtggttacta tatccgcctt
     9421 ctaaatcaat aatatgattg aaaatgttct caaacagttc cacatcataa atatctgtca
     9481 ttaaatcacc acccgaatat tttcactgca gtgaacccta aagagataat cgctattatc
     9541 caacctgacc acttaataat ctgatcacct attttatttt ttgctaattt ttcagtttca
     9601 atataatcaa tccgtttttt gatgctcaac aaatcctctc tcaaaccgtt atacttcccc
     9661 actattctta ttgtgttatc taatttattg gaaaggactt cagtttgggc ggtaagtcta
     9721 actattagat cataaaggtc cttgttttca taccactcct tctgcccggt cttttttata
     9781 atctcttcaa tttctttttt aaaaggacag gtatcttttt catgtttttc catataatca
     9841 actccaatct attttaaaat tgtgggctgt aaatccatgg tgccaatagc aatagtagtt
     9901 ctaaggtttt cactatcaaa gacctctaat tcacagtaat atttttgctc ttttaagttt
     9961 aaagtatctg cggaaaataa aataaccgac acaatattac ttacagtgtc gattgcaatt
    10021 tcacccgtta tatttgaatc ttttgttaac atggccgctc cagacctgct cctagaaata
    10081 gcccattttg ctgctgttaa gtcctcagca gcttgtaccc catcaacatt ataatttaac
    10141 ttaaaatcat cgccctgcca aacctcaaaa tcttgatttt tataagccac ttattgatca
    10201 ccacctattc tgccctgcag gctgatggtt tttataatac tagagtttag atccttctct
    10261 ttttctaaag atgcattcaa acttaactct tttttaattt ctgcttctaa agatacaata
    10321 tttatgatca aaggaatctc tgtgcttaat tctttagaca caacctcaga aggatttaag
    10381 acctcatcat agattaaagc ataaatggtt aaagaaccag aactggctcc cagagagtta
    10441 agggaaagag tgctcactag agaatgagta tttaatgaat aacttaaatc aaatctggtt
    10501 cccaaactat caattaaata aagttcgtgg atataagcgg cttttaatgt ttcgtcagaa
    10561 ttaacagtta ttgtaaattc ctcttccata tctacgaacc ccggtgacac aatttctgca
    10621 gctggacctt gagtatctaa agccaatata aaataagcac tcatctaatc ccccctaacc
    10681 taatttatta tacagaccag gccccgcttt tatctttcgc aaaaacctta actatttttt
    10741 caccatcacc cgcacttgca atttctaaat cagaaccata cacctgacag ttaatagcgg
    10801 tctcagccgg ataatcaccc gcaacccccg acatattagt tgatccatta gtagttccga
    10861 taagagttcc tgtgtcatga gcagccccag ttgatgctac aaccttaacc ttatattcat
    10921 caaagatttc attaacagta aagctaaaag aactcacatt tgcagttgca atcttagaaa
    10981 ttttattcac atcaggacca gaaatagtaa ccaccggtag tgaagtgtct aaagtaatag
    11041 aatcactggt ttgagctgat tcgtttaaga catcatccct tatttttaaa tataccgttt
    11101 ttgccccatc gccaatagac aatttaacct gctgtgtagc tgaataagtt atccaacttg
    11161 agttgacttc tgtgtcctga atattcgggt tttcagttag atcgacatct ccccagattt
    11221 tcatctgata acctgtggtc tccccatctg cagttgaaat atctgcatta attaactgct
    11281 gagttgcata ctgagctcca ctttctaatg agatagcagg actctgcggc ccggttgtgt
    11341 ccaaagttaa taaaaaataa tttgccattt taaagcctcc ctttctttat attttatcta
    11401 tgctaaatgt caaagcatat ttatatttta aataatcaaa tattccccta atttgttgaa
    11461 atccacctat tgcggtattt ccattagtgg aaatgaaata ataataccct ttaaccttat
    11521 ataggcaaac tacatggcct ttccaaaact ttaatgggat aatggttaat agataaggat
    11581 tataacctaa ttgctctaaa agtcgataag ccagcataga aaagtcatca caatcaccct
    11641 tatatccact tttcactgta taatctaaat cctgaatagt atctattatt ccgaatagtt
    11701 tatcactttt ataagtcatt tttttattta caaacctacc cacatcaaaa tgatctttta
    11761 aattttcttc ttctatcagc attttagaca ttttttcaga agcttcttta tcacccttta
    11821 taaacttgta ttttaaattt aaatacaatt ttaacaaaat aaaggcaact cctttcctaa
    11881 ctatctgacc ataggccgtc tgaatttttg ccataaacat ttatttgata ggttttatct
    11941 ccttgcgtta attcggtata atcgacatca aaagtgacat cagtattggc agttacagaa
    12001 ccaccgctac caacaagcag acctgaacca tgacctgtcc cgtctgctct cgcttcccac
    12061 tccactaaat cctggtcgct ttggaaagta accgttgagg tctgataacc aacttcgtct
    12121 gaaatcttat cttttgtgta gcccacaata tttatggtcg gtaaatttga agtgattata
    12181 gaaatcggcg ttataacttc agcatcaata gtgccggttt caaaagtcat actatttgat
    12241 gaatgttcat ctacattctt ataagaaaat ctgacaacta atatatcacc tgcatccccc
    12301 gcagtccaag tacaattata aattcccatt ttataaacgc ctgttccttg tgaaatataa
    12361 taggtgctgt catcctggtt ttcttcctgt agcacaccgt tggaatttct tctttgtatg
    12421 ctcatcgtta ttgccgtgtc ttttgctgga acagtagtca cattaatttc tacattaatt
    12481 accccatcct cccaatgagg gttatttgga ataccagcct ctgtataaaa aaagccgtga
    12541 taaaaattag tatctttatt gggtgcagtc accgttattg aatcagcagg cagagtagtt
    12601 aatgataaga gtttatcaaa ataatacccg ttagaataac tagcatcagc attacttaaa
    12661 taaaaagtcg ccatctctaa ctctctcctt tcagccagaa atccattatt gctttctcat
    12721 ttatttcctt aaatttttct ttatccatat accagccata ctttaatgaa ttttttggaa
    12781 ttaaagagaa ttttctcgtg tcattacatg ccataaaatc gattccatcg aatagataat
    12841 aatcaaaacc agatatgctc attctgtaat gctttttgtc tgctccaaaa tacttatttt
    12901 catatagcat tataatttgt atattttcaa agggtgccct atgccactct ttaaataagt
    12961 gtagatatga ccccttggca gtaaatacaa aaccatctcc gtaatataat ttataaccct
    13021 ctattttgat gtctttcatt tttgatctat gcttaactag aggctgttta atataatcta
    13081 ataatttctg atcggccttt ctcatatcat caccaacttt ttttataaat caattccata
    13141 ttccatctgt tttcactgct gacaataaaa ccaaatacca attccctccc gacttaacat
    13201 aagcttccgc tgaatgccat atcccgcttt ttttaaccca aaaaactcca gtttcaggct
    13261 ccggccacca gtaaccatca accgctttta aggccggata tccatcattg acctcataaa
    13321 taacaaactc gacagaatca gtctcaggat caactccagg cggataatta taagaattag
    13381 ctgggggcat agtgcccata tcccaatcaa tgtaagtgct ccccaggtca aagggataag
    13441 tcatttcttc ggtagtcctc ggaacgccct tcccggtatc gctttgcccg gctgtctgca
    13501 gatcataata aactttttga ataataccag aattataacc gcaaaaacca cccacattaa
    13561 gactgccgtt tacagaaccg gttgaatagg catcagctat aaagctatgg ttataacccg
    13621 catatcctcc cacataatta ttgccaacaa cagaacaacg ggaataatta taataattat
    13681 cattgtaatg attcccaaca aatcctccaa cataatttgc acttgctgca gttatagaac
    13741 cctttgtgaa tgtcctatta acctgtccca gataactctg tccgatataa ccgcccacat
    13801 atttaataat ccccggaccg tttatagtaa cctcaacctc attaaaagaa tgagttgttg
    13861 aagctccggc acttccggct tcaagcccgg ccattccccc aacacattca gcaacagggc
    13921 tgccattata ataaagctct gcatttatag tcccctgaac attgcaatat tgaactgatg
    13981 agcaggtaac ataactaaat accagggcaa tgtagggtaa attagaagtt actgtaatat
    14041 ttttaaaatc tattttatat aaagttggcc cttcagtttg atccgaccgg cctgaatttc
    14101 taaaaaaagc atttgacatg taatttcttt cgcgaattgg aatcgcaagt gacttgtttg
    14161 ttatttggtt aagttcagca ttataaattg ttctgttcgc ataagaacca gtagcagtat
    14221 cataaccaac gaactgtgcc gtagtccaac ttgctggaat ccaattaccg taatctgcca
    14281 ggtgatctgc tctatgcctg aaattataat ctgtcaagtc cagatcgaca gataaataaa
    14341 aagtataaga gggataatac ctgatctgcc acaagcggtc cagagtgttc acattattgc
    14401 tcaaagaaat atattgaata ttaaccgtat gagtctggcc cccctggggt gttataatct
    14461 gctcgttggg atcatacccg acattcctcg gccagggtga aatataatgc tcaactgctt
    14521 ctaaataaaa agaataggtg ccattcccat cagttgacac atcaccaccg ttcgctgcaa
    14581 tctctgtgtt atccggggca tcaatatgtg ggaatcgttc ccatttgtca aacccaatca
    14641 cctgcaggcc cgaccaatta accggactcc cagaaggatg agttatcgcc ccctgaatta
    14701 gggcactccg atattgttcg gtgtcaatgc ttgcgtcact gtgcataaag atcatatttg
    14761 tggtattgac cgtctcataa ccatcaaact gagcttttaa atactgcctt aaaattatat
    14821 gaccggaatt agtccccgac caggtagcag ttaaagttgg actggccggc tccggatcaa
    14881 tagattgcca gaacttaaca ttaatcgtgt catcgataag ttcaatttta actaaataat
    14941 actgcaacat atcacccgca tactcgatca cattgggcgt gctgacacta tttatggaag
    15001 tgtggctatt tgtggatgaa gtatactgca taagatgaat gcttaaagta gaccagtcat
    15061 agatcacttt aaaataaaga tagtcggttg cattactgta attctggccc gcccttaaca
    15121 ttatttcttc ctgtccggtt tttatgcagg gtgtgccagc cggaatccca tgttcggctg
    15181 cctgttcatc ccccaactcg ataggtgatt caaacttaca aaccaaagtc gtgttatata
    15241 cactgcccgc tttgatccat ctaacaggcg gactgtcggt gtaacctaac tctgccataa
    15301 tactgtgacc tcttacaccc atatattcat ctatcgcctg ggccatattt tgggaaggat
    15361 tccagaccgc aaagtcatta taaggccttt catatggttg atattcctca aaaagggtaa
    15421 actgactata agccattatc tttcaccttt tcttttacag agatctccgg caggctttca
    15481 atttctgctt tatttatctt aaccttgtct agcttaaaga gatcagactt aattctttta
    15541 ttaccattaa ttagaattgt tttatcagca ggcaccgtca accccccttt ttaagaaatt
    15601 aagattttcg gctaaataag ccctttattc ataagactta atagcacctt ccaaaatttt
    15661 tcactccaac ctgaaaccaa tattttccac tatcattaaa cccttcaaaa agcagataaa
    15721 ctgcgattaa cacatcat
//
LOCUS       DATGCJ010000154         5330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_153789, whole genome shotgun sequence.
ACCESSION   DATGCJ010000154 DATGCJ010000000
VERSION     DATGCJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153789"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VKN64_07735"
     CDS             <1..306
                     /locus_tag="VKN64_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HMA60169.1"
                     /translation="LAPTQVVVLPVSDDQADYAYDIKDKLFADGVRVEVDDSNEQLGY
                     KIRQAQLEKIPYMLIVGSREAEEGTVSVRDRDEGDLGAVEFVEFKDKILKEIENKVK"
     gene            513..1040
                     /gene="infC"
                     /locus_tag="VKN64_07740"
     CDS             513..1040
                     /gene="infC"
                     /locus_tag="VKN64_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMA60170.1"
                     /translation="MSNNNDLRVNEQIRAKEVRLIDNEGEQIGIQSLDDALDKAEERG
                     FDLVEVAPQANPPVCKIMDYGKYKYEQAKKRQKQKKNQNVMKVKEVQMGVKIQDHDFN
                     VKLKQARRFLNDKNKVKVRIRFRGREMVHKNLGYDLMDRLKEETDDIGKVESGPKMEG
                     HNMLMFLTPVSDKSN"
     gene            1059..1256
                     /gene="rpmI"
                     /locus_tag="VKN64_07745"
     CDS             1059..1256
                     /gene="rpmI"
                     /locus_tag="VKN64_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMA60171.1"
                     /translation="MPKLKTHKGIKKRVKVSKHGKIKRRKAFKSHILTKKSSKRKRHL
                     RKPTTMDKTYQKRYKKLLPYE"
     gene            1312..1665
                     /gene="rplT"
                     /locus_tag="VKN64_07750"
     CDS             1312..1665
                     /gene="rplT"
                     /locus_tag="VKN64_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMA60172.1"
                     /translation="MARVKRGSKARRRRKKVLKSAKGFIGSRSTLFRPAKQAVIKSLQ
                     YAYRDRRQKKRNFRRLWITRINAAARQNGLTYSRFINGLKQANVEIDRKMLAEMAVND
                     KDAFSELVEVAKSEL"
     gene            1722..2498
                     /locus_tag="VKN64_07755"
     CDS             1722..2498
                     /locus_tag="VKN64_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMA60173.1"
                     /translation="MEEITSIKNDKVKYLRKLYQKKYRKERKQFILEGLRLIRGAYKS
                     GADIASIYLTRDYYNEHKSESFLINNEEKLIFVSDSLISEVADTENPQEVIAIVNEPQ
                     TSQEEVLSKDYILVIDQIQDPGNMGTMIRTAAAAGFQSVIISKGSVDVFNLKVLRSTV
                     GAIYSLPFIKDIELDDLKEMLKSKKQNIYAADLNTDKYYNDIKYVKPLSLIIGNEGNG
                     IRRQLIDIADVCVKIPLKGNIESLNAGVAGGIIMYKILEN"
     misc_binding    2726..2952
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2998..4023
                     /gene="pheS"
                     /locus_tag="VKN64_07760"
     CDS             2998..4023
                     /gene="pheS"
                     /locus_tag="VKN64_07760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMA60174.1"
                     /translation="MDLLEEIKKIEKEVKEKFENVKDLDQLEELRVEYLGKKGKISQI
                     FSKMGQVDDEKKPIVGKETNILKNKAQNMYEEIKEKKEKELKQKRLKAETIDITLPGA
                     EVNVGHKNPFTHIIDEVVELFLGMGFSIEEGPEVENEYYNFEALNIPEDHPARDLQDT
                     LYIDDQYLLRTHTSPVQIRTMEKQDLPIRIIAPGRVYRSDELDASHSPNFHQIEGLVI
                     DKDISFSDLKGVIAEFVHEIYGEERKIRFRPSYFPFTEPSAEVDVSCNVCKGAGCSFC
                     SYTGWVEVLGSGMVHPNVLRMSDIDPDLYSGFAFGMGWDRIAMLKYDIDDIRVLYEND
                     KRFIHQF"
     gene            4043..>5330
                     /gene="pheT"
                     /locus_tag="VKN64_07765"
     CDS             4043..>5330
                     /gene="pheT"
                     /locus_tag="VKN64_07765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMA60175.1"
                     /translation="MQVPYNWLKTYIDFPYTPEELSHKLTMAGLEVEEVEYLGTGLEN
                     IIIGEIVEINNHPNADKLKICQVNTGDKTLQMITGAPNVRSDIKVPVAEVGVTLPTGM
                     EIEKTKLRGEVSQGMICSKDELGLQEERASGIMILPDKAVVGQPFVEYYGLDEYVFHL
                     DLTPNYARCLGMLGVAREVKAMQKSSSQIDWPEIDIKDDDRTSIDKLASIKIEDEDLC
                     PRYAGRVIRDVTIKPSPKWMQRRLKAAGIRPINNIVDITNYILMEYNQPLHAFDLDKI
                     KDGKVIVRRAEENEKVVTIDDVERTLDQETLVITDPEKVIAVAGVMGAENSEVTDQTT
                     DIFLEAAYFEPTSIRKTARKLGLPSEASHRLERGIDIERVIEAGNRAAYLMQEYADGK
                     VAQGVIDKYPVPYQENIIDVNLAKVNRLLGIELNKDE"
BASE COUNT         1807 a          898 c         1255 g         1370 t
ORIGIN      
        1 ttggctccta ctcaggtggt tgtcttaccg gtttccgatg accaggccga ttatgcctat
       61 gatataaaag acaagctgtt tgcagacggg gtccgggttg aagttgatga cagtaatgaa
      121 cagctcggtt ataagataag acaggcccag ttagaaaaga taccttacat gttgatcgtt
      181 ggttcacggg aagctgaaga aggtactgta tcagtccgag atagagatga gggagacctc
      241 ggtgcggttg aatttgttga gtttaaagac aagattttaa aggaaataga aaataaagtg
      301 aagtagagtg ttgacatact ataacgacct gtggtacaat ttaattcgac aataaagtag
      361 aaaccactgt ttctcaccaa taataggatt attggttaat agttagtagg tattacttga
      421 ggcgatttat gtctcctaac tttatgtgta tgcaaacggg tggtttctac cacccgtttt
      481 ttattgtcca aaaaaaagtg gaggtgcata aaatcagtaa taataacgac ttgcgtgtca
      541 atgaacaaat tagagctaaa gaagttaggt taatcgacaa tgaaggagaa cagattggaa
      601 tccaaagttt ggatgatgcc ttagataagg cagaagagag aggttttgac ttagtggaag
      661 tagctccgca ggcgaatcca cctgtctgta agatcatgga ttatggtaaa tacaaatatg
      721 agcaggctaa aaagagacag aaacagaaga aaaaccagaa tgtaatgaag gttaaagagg
      781 tccagatggg tgttaaaatc caggaccatg actttaatgt taagttaaag caggctcgta
      841 gatttttaaa tgataaaaat aaagtaaagg tacgcattcg attcaggggg agagagatgg
      901 tccataagaa tctaggttat gatctcatgg atagactcaa agaagaaaca gatgatattg
      961 gtaaagttga aagtggtcct aagatggaag gccacaacat gttaatgttt ttaacccctg
     1021 ttagtgataa aagcaattaa agtaaggagg tattattgat gcctaaactt aagactcata
     1081 aaggaataaa aaagagagtt aaggtttcta aacacggaaa gataaaaaga cgtaaggctt
     1141 ttaaaagcca tattttaacc aagaagtctt ccaaaagaaa aagacattta agaaagccta
     1201 caacaatgga taagacatac caaaagagat acaagaaatt attaccctat gaataattga
     1261 atataaaata atcaaatatt ttgatataga cggaaaaagg agtgattaag catggctcgt
     1321 gtgaaaagag gttcaaaagc acgcagaagg agaaaaaagg ttctcaagtc ggccaaagga
     1381 tttattggtt caagaagtac gctgtttcgt cctgccaagc aggctgtaat taaatcctta
     1441 cagtatgcct atagagacag acgccaaaag aagcgtaact tccgccgcct ctggattacc
     1501 agaattaatg cagcggcccg acagaacgga cttacctatt caagatttat caacggtcta
     1561 aaacaggcta atgtagagat tgatagaaaa atgctagctg agatggctgt aaacgataaa
     1621 gatgccttca gtgaactagt tgaagttgca aaatcagaat tataatacat tattttaaaa
     1681 acccggtgtt tgccgggttt ttagtatatt aggtgatcat tatggaagaa attacaagta
     1741 tcaagaatga taaagtcaaa tatttaagaa aactctatca aaaaaagtac cggaaagaaa
     1801 gaaagcagtt tatcttagag gggctgaggt taatcagagg tgcctataag tccggggccg
     1861 acatagccag catctacctt acccgggatt actataacga acacaagagt gaatcatttc
     1921 taattaataa tgaagagaaa ctaatttttg tatccgacag cctgatttct gaagttgccg
     1981 ataccgaaaa tccgcaggaa gtaatcgcga tcgtaaatga gccccaaacc agtcaagagg
     2041 aagtattgtc aaaagactat atcctcgtaa ttgaccagat ccaggacccc ggtaatatgg
     2101 ggactatgat caggacagcg gctgcagccg gtttccagtc cgtaataatc tcaaagggat
     2161 ctgtcgatgt ctttaattta aaggtcttaa gatcgacagt gggtgcaatc tattccctgc
     2221 cctttatcaa ggatattgaa ttagacgatt taaaagagat gctgaaaagt aaaaagcaaa
     2281 atatctatgc ggctgatcta aatacagata agtactataa tgatataaaa tatgtaaagc
     2341 cactttcctt aattatcggt aatgaaggta atggaatcag gcggcagctg attgatattg
     2401 ctgatgtatg tgtaaaaatt cctcttaagg gaaatattga gtccttaaac gcaggtgtag
     2461 cgggcggtat catcatgtat aaaatattag aaaattaaag caatttattt tattatcctt
     2521 gtcaaaacaa ggaacttgtg atataatata aaaaaattaa agacaactat ctctgtgaaa
     2581 ggatgtggtc ctttttgggc gtagctgcat cattgttctg gaaattgttt gaggaaaccg
     2641 ggtcggtcaa tgcatatttg gcatacagga gaatgtttag gacagaaatt agaagaggta
     2701 gttagtaagt aagacccaaa cataactaaa ggctgtgaag gagagagtag ttacaaaaac
     2761 ttaaaacagg gagagactgt ccaaggactg agaacggtct tattaaggtt ttgtaatgaa
     2821 gttcactcct gagccgagga ctgaactaat agtaggtctt atccgggtaa ttccgttaaa
     2881 gattccaagt gatcttatca cctttatgtg gtaaggtaac tagggtggta ccgcgttcat
     2941 ctcgtccctt cgtgaggacg agtttttttt atgcttaaaa ctacggaggt gttaaaattg
     3001 gatttattag aagaaatcaa aaagattgaa aaagaagtaa aagaaaagtt cgagaatgtt
     3061 aaggacctcg accagttaga agagctaagg gttgaatacc tgggtaaaaa gggtaagatt
     3121 tcccagatct tttctaaaat gggtcaggtt gatgatgaaa agaagccgat cgttggtaaa
     3181 gagaccaata tattaaagaa caaagcacag aatatgtatg aagaaatcaa ggaaaaaaag
     3241 gaaaaagaac taaaacaaaa aagattaaag gccgaaacga ttgatataac cttaccgggg
     3301 gctgaggtca atgtcggtca taaaaacccc tttacccata ttatcgatga agtggtagaa
     3361 ctatttttag gtatgggctt ttcgatcgaa gaaggtcccg aggtagaaaa cgaatactat
     3421 aacttcgaag ccctgaatat cccagaggac catcctgccc gcgacttaca ggataccctc
     3481 tatatcgatg atcaatacct actgaggacc catacctcac cggtccagat tagaaccatg
     3541 gaaaaacagg acctgccgat aaggattatt gcgccggggc gcgtctaccg ttcggatgaa
     3601 ctggatgctt cccattcacc caacttccat cagattgaag ggcttgtcat tgataaggat
     3661 atctccttca gtgacttaaa gggtgttatt gccgagtttg tacatgagat ctatggggag
     3721 gaaagaaaga tcaggttcag accgagctac ttccccttta ccgaaccgag tgcagaagta
     3781 gatgtttctt gtaatgtctg taaaggagcc ggttgttcct tttgttccta tacaggctgg
     3841 gtggaagtat taggttcagg tatggtccat cccaatgtct tgagaatgtc agatattgac
     3901 cctgatctat acagcggttt tgcctttggt atgggctggg accgaattgc aatgttaaaa
     3961 tatgatatag atgatattcg agtactgtat gaaaatgaca agagatttat ccatcaattt
     4021 taacaaagga gtgtgagata agatgcaggt accatataac tggttaaaaa catatattga
     4081 cttcccctat acccccgagg agctgtccca taaattaacg atggccggcc tggaggtaga
     4141 ggaagtggag tacttaggaa caggcttaga aaatattatc attggagaaa ttgttgaaat
     4201 aaacaaccat cccaatgccg ataagttaaa gatctgtcag gttaatacag gtgataagac
     4261 cctgcagatg attaccggag cccccaatgt gaggtccgat ataaaagtac cggtagccga
     4321 ggtaggtgtt accttaccga ccgggatgga gatcgaaaag accaagctca ggggcgaggt
     4381 ctcgcaaggg atgatctgtt ctaaagatga gctgggtctc caggaagaaa gggccagcgg
     4441 tatcatgatt ttaccggaca aggccgtagt cggccaacct tttgtggaat actatggttt
     4501 ggacgaatat gtattccatc tggacttaac acccaactat gcccgttgtc tgggtatgct
     4561 aggtgtcgcc cgggaagtta aggctatgca gaaaagcagc agccagattg actggcccga
     4621 gatagatatt aaagatgatg acaggacaag tattgataaa ttagcttcaa tcaaaataga
     4681 ggatgaggac ttatgtcccc gttatgccgg acgtgtgatt agggatgtta cgatcaagcc
     4741 ctctcctaaa tggatgcaga ggcgcctcaa ggcggccgga atcaggccga tcaataatat
     4801 agttgatatt accaactata tattaatgga gtacaaccag cccttacatg cctttgatct
     4861 tgataagatc aaggatggaa aggttattgt cagaagggcc gaggaaaatg agaaagttgt
     4921 tactatcgat gatgtagaaa gaacccttga tcaggaaact ctggtcatta ccgatcccga
     4981 aaaggtaatt gccgtggccg gtgtgatggg ggccgaaaat agtgaggtaa ctgatcagac
     5041 taccgatatc ttcttagaag cggcctactt tgaaccgacc agtatcagaa agaccgcccg
     5101 taagctgggt ctacccagtg aagcttctca ccgcttagaa cgcggtatag atattgaacg
     5161 ggtaatcgag gccggtaacc gggccgccta cctgatgcag gagtatgcag atggtaaggt
     5221 agcccaagga gttattgata aataccccgt tccttatcag gaaaatatta tagatgttaa
     5281 cctggccaag gttaaccgct tactggggat cgagttaaat aaagatgaaa
//
LOCUS       DATGCJ010000155         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_464920, whole genome shotgun sequence.
ACCESSION   DATGCJ010000155 DATGCJ010000000
VERSION     DATGCJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464920"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1384)
                     /locus_tag="VKN64_07770"
     CDS             complement(212..1384)
                     /locus_tag="VKN64_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA60176.1"
                     /translation="MIEGKENEMSSGVLKYGMVGGGSGSFIGGVHRKAAVFDNKACLV
                     AGCFSRDYQNTKNTGKSLGLTEDRLYETYAEMAEQEAQREDPIDFVSIVTPNHLHYDV
                     AKKFLEAGINVVCDKPLTFTYQEAEELDKLARENELLFCVTYTYTGYPMVKHAREVVK
                     RGDIGDIRVVIGEYSQDWLATLAEKEGNKQAAWRTDPKKAGRANCVGDIGSHLENMVS
                     YITELEIDSLCANLDHFGKGRELDDNAEILVKYKGGATGVYWCSQVAVGHDNDLKVRI
                     YGTNGSIEWHQEEPNKLKISYLDKPTEILSRGRDELYPLANDLPRIPAGHPEGYFVAF
                     SNIYTSFEETLLDKKSGTAYQDLNIKFPTAREGAQGVKFINKTVDSDEANSTWVKC"
     gene            complement(1471..2655)
                     /locus_tag="VKN64_07775"
     CDS             complement(1471..2655)
                     /locus_tag="VKN64_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HMA60177.1"
                     /translation="MDNIKIGNSSFIKNLNQSSILRLINEYGPISRKELSEKTEYSAA
                     TISNHVKTLIEEGFVIEKEKGISSGGRKPVFLTINPNKGFIIGVDIEVNKVKIVLFNL
                     KLKVEKKNEFYIETDKTPEQILEKILSHIGKILKEKRIEEYDLLGIGVAVPGLVNKDR
                     NILQFAPNLGWEKEKISEYLNRNYKVPIFVENEANAAGLGEKEFYYDKYINMVYVSIN
                     EGIGCGLIFNGELYRGASGNAGEFGHIIIESESERTCHCGNKGCWEALASENYIYDRY
                     SKITKRELINIDQVYKKIKENNTDEEIRKLLDKVGENIGLGIANIINSLSPELVIIGG
                     NILKIKDYLDQPLQRSIREKALRFNYDKVKVKYSKLGELATVYGIAKIIFNRNMLVKE
                     GS"
BASE COUNT          856 a          628 c          348 g         1103 t
ORIGIN      
        1 cagttccttt ttgcttttta catatttcat atcttcagct gcttttttga catccatatg
       61 gtccccccag caggcaattt ccaaaccatc ataaccaata tcactcatca gtttacagac
      121 ttcttcaaat tctaaatctg cccattgtcc tgtaaagatc gtaactggtc tacccatctt
      181 tattttcctc ctttaatgat taaaaataat gttaacactt aacccaagta gaatttgctt
      241 catcactgtc cacagttttg ttgataaatt taactccctg ggcaccttcg cgggctgtcg
      301 ggaacttaat attcaaatct tgataggctg taccgctttt tttgtccagt aaggtctctt
      361 caaaactcgt atagatatta gaaaaggcta cgaaatagcc ttctggatgg ccggctggta
      421 tcctgggaag atcattggca agtggataaa gttcatcccg gccccttgat agtatttcag
      481 ttggtttatc aagataagaa atctttagtt tattaggttc ttcctgatgc cattctattg
      541 agccgttagt tccatatatt cttactttca agtcattatc atgaccgaca gcaacctggg
      601 aacaccaata aactcctgtt gcccctcctt tatacttaac taagatttct gcattatcat
      661 ctagttctct gcctttccca aaatgatcta gattggcaca gagactatct atttcaagtt
      721 ctgtaatata ggaaaccatg ttttctaagt gactgccgat atctcctaca cagttggccc
      781 ggcccgcttt tttgggatct gtccgccagg cagcctgttt attaccttct ttttcggcta
      841 aagtagctaa ccaatcctgg gaatactcac cgatcacaac cctaatatcg ccaatatccc
      901 ctcttttaac aacctctctg gcatgtttaa ccattggata accggtataa gtataggtca
      961 cgcaaaaaag aagttcattt tccctggcta acttatcaag ttcttcagct tcttgataag
     1021 taaaggttag aggtttatca caaactacat taatgccggc ttctaaaaat tttttagcga
     1081 catcataatg taaatggtta ggtgttacta tcgagacaaa atcaatcgga tcctctctct
     1141 gcgcttcttg ctccgccatt tctgcatatg tttcatatag ccgatcttct gttagcccta
     1201 aacttttccc ggtattttta gtattttgat agtcccttga aaaacaacca gcaaccagac
     1261 atgccttgtt atcaaatacg gccgcttttc tatgtactcc gcctataaat gaaccagaac
     1321 caccaccaac catgccatac ttcaaaacac ctgacgacat ttcattttct tttccttcta
     1381 tcatcttgat ccccctttag aaatataaaa tatatttgtg ttaattttaa tatatttgct
     1441 aattttctga cttttttatt aaatataaat ttagctccct tctttaacta acatatttcg
     1501 gttaaagata atcttcgcaa ttccataaac agttgctaat tctcctaatt tactgtactt
     1561 tacttttact ttatcataat taaatctcaa ggccttttct cttatcgatc tttgcagcgg
     1621 ttgatctaaa taatctttta tctttaagat attgccacca ataattacaa gctccgggct
     1681 taaactatta attatattag ctattcctaa accaatattt tctcctacct tatccaataa
     1741 ttttctaatt tcttcatctg tattgttctc ttttattttt ttataaactt ggtctatatt
     1801 aatcaactct ctttttgtta ttttactata tctatcatag atgtaatttt cggaagctag
     1861 tgcttcccaa caacctttat taccacaatg gcaggttctt tcagattcgc tttcaataat
     1921 aatatgacca aattcccccg cattaccaga agcacccctg taaagctcac cattaaaaat
     1981 tagaccacaa ccaatacctt cattaattga aacataaacc atattgatat atttatcata
     2041 gtaaaattct ttttctccca acccggctgc attggcttca ttttctacaa aaatagggac
     2101 tttgtaattt ctattaagat actcactaat tttttctttt tcccaaccca aattaggggc
     2161 aaattgtagt atatttctat ccttatttac caggccgggc accgccaccc ctatacccag
     2221 tagatcatat tcttcaattc ttttttcttt taaaattttt ccaatatgac ttaatatttt
     2281 ttctagtatt tgttcaggtg ttttatcagt ttctatataa aattcattct ttttttctac
     2341 ttttaatttc aaattgaaaa gcactatttt aaccttattt acctcaatat ccactccaat
     2401 gataaatcct ttattgggat ttatcgttaa gaatacaggt tttctaccgc ccgatgatat
     2461 acccttttct ttttcaatta caaaaccttc ttcaatcagt gttttaacat gattggagat
     2521 tgtagcagca ctatattcag ttttttctga caattcttta cgtgaaatag gtccatattc
     2581 attaatcagt cttaatatac tggactgatt aagatttttt ataaaggatg aatttcctat
     2641 tttaatatta tccatattta ttatcctttc aacaacttaa tttaaaactt aatttaactc
     2701 ttaaaagaac ttacactata taattatctt tgtcttaata ttttcctgca taaaaattaa
     2761 aataatttta tatataatta aatatatttt cataaaaaaa agaccagtaa atactggcct
     2821 caaagtttaa caatcatatt cttttatttt tttaaatatt tcctttacag atagaatttt
     2881 attaatcttc catagtcctt tacctgtaaa aaatacccct tcttctaaat ctccc
//
LOCUS       DATGCJ010000156         9512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_65003, whole genome shotgun sequence.
ACCESSION   DATGCJ010000156 DATGCJ010000000
VERSION     DATGCJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65003"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VKN64_07780"
     CDS             complement(<1..510)
                     /locus_tag="VKN64_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60178.1"
                     /translation="MKKIIIVFIVAVLLFAAFNIFNISIYNYLDRDYDDLIAKDYNGV
                     NIDEVDNYNIDIKFEPEQRLCKIEQTVDYINKENTDLNQVYFHLYPNAFRENIIGLGL
                     VENVNPDYEPGYIEFSEIKIKGEVIDFEIIGADKTLLKIELDNILKPKERLKIIMEYT
                     LKIPNLEHRF"
     gene            complement(551..1426)
                     /locus_tag="VKN64_07785"
     CDS             complement(551..1426)
                     /locus_tag="VKN64_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HMA60179.1"
                     /translation="MRDKERVLEDIIKFAKQNPDIEALLITSSMANPRVETDVFSDLD
                     VVLVTKEPKSLLKDKSWINDFGEVMVAFNDNFKLEDITTYTSLNIFQDYIRIDFSIWP
                     IELVKKVSNYDKLPAFLDIGYRVLYDENNITVKLNEPTHNAYQTKKPNQEVYDRVVNN
                     FWWDITYIAKYLWRDQFYFAKYMDYFIKYNLLKPMIEWLIGVDNNWQVNPGKQGSNFK
                     KYLDDKLWKELKATFSGGDINDNWESTFKMMDFFSKISKKVAYRLRFEYPQSLEDEVR
                     DYLNKIYNKEVPFDK"
     gene            complement(1442..2377)
                     /locus_tag="VKN64_07790"
     CDS             complement(1442..2377)
                     /locus_tag="VKN64_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60180.1"
                     /translation="MKEQRVVYWLLQGDPAIRYQVYRDILETEPQKLQVLRNEMVTKG
                     WVKRLLDEQAEDDIWGGGLYSPKWISSTYTLLLLKRLSLPSDNSQARRGTVILLDRGL
                     YKDGGINFSKTLTRSETCITGIVLSLLSYFKVKDERIKNLIDYLIAEQMHDGGWNCKK
                     HKEAKHSSMHTTINVLEGLLEYKNVFDYKKEEVISMEKEAHEFLLQHRLYKSDKTGEV
                     IDKKFTYLSFPPRWYYDILRVLEYFYFYDKDYDERMEDALQLLLKKKNKACKWPLQGR
                     HSGKEFFKLEGNTRESKINTLRAIRVLKKYNISLN"
     gene            complement(2402..2866)
                     /locus_tag="VKN64_07795"
     CDS             complement(2402..2866)
                     /locus_tag="VKN64_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMA60181.1"
                     /translation="MFIEMRRKEKQLAKVKALEILKKGEYGVLATIGENDYPYALPMN
                     YVFFNDKIYLHCAKEGHKIENINYNNKVSFTVVGDYELQPAGFTSNYESVVIFGRANS
                     LNGEIKKKALQQFILKYSPDFKKEGFDYIEKAIDKTEIIEIEIDDIKGKKND"
     gene            complement(2933..3526)
                     /locus_tag="VKN64_07800"
     CDS             complement(2933..3526)
                     /locus_tag="VKN64_07800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60182.1"
                     /translation="MDKDKKEEIEFFNKHAEEWDEFITDENIEVAKEVIKKYKIGRNN
                     SVLDVGAGTGILYSLLKNENLSGYLGIDISPKMIEVFKEKHPDAEIRRIDFETNVELD
                     KRFDFVIIYDTIPHLDKIDMVFKNARKHLKVGGQFLIIHSKTRKELKEHHDEISHNQE
                     DPIPTDEVLKLMSWTYNFEDTKIKDEDYFLYSGKKKR"
     gene            complement(3562..4449)
                     /locus_tag="VKN64_07805"
     CDS             complement(3562..4449)
                     /locus_tag="VKN64_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60183.1"
                     /translation="MKDLLSIKDLSKSEVYEMFADTKRLKIKNHNGNDLRPLLHKTII
                     TTFPSTSLRTKISFESAINQLGGDIINLSLDLEGKEELADKVKYLNNWVDYLIIRYPE
                     QDTIEKIAKLADFSVVNAMSKKYHPCEVLSDLFTLYQLKGNLNNLKFVFVGEGANISN
                     SWFNAAGVLDLNLTQVCPEGFEVKDDIYNFAAENSEGIIERTNDLQTGIRGADVILTD
                     AWPPHGYKTQDKFAQYQINLENIGYASNDCIVNPCPPFTRGEEVSKEIIESDYFIGYK
                     AKENLLHMQKTILMNLEND"
     gene            complement(4548..5744)
                     /locus_tag="VKN64_07810"
     CDS             complement(4548..5744)
                     /locus_tag="VKN64_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMA60184.1"
                     /translation="MRRDLKRLTEKHLEYFCNNLGDRYVGSKSNQQAASYIKENLKDV
                     EIEEQTFDCFDWYPNDLIFKVDDTEYEAQISPYSPVVELKAELVTASNIEQLEKLEGK
                     DKVVLLSGELVKEQLVPKNFPFYQVEEHQTIIKLLEEKGFKAVITATGKDQSTAGGLY
                     PFPLIEDGDFLIPSIYIKDMTGEEIKKQVGKDVYLKIDATKVDAKGKNVIAKVGKKNK
                     DKIVLFAHFDSKVDSPGAIDNATGVTTLLLLIELLKDKNLDKQVEVALLNGEDYYSNP
                     GQRVYFIDNKSRLENIYLGINIDGMGYKKGNTAFSVYTTDKNINNLSDKIFKNKYSMM
                     KGEPWYQGEHAILVQRNIPALAFTSNLLEEILDKVHTEEDNLDIIDKRKVLDTAFALS
                     EFIIEV"
     gene            complement(5778..6314)
                     /locus_tag="VKN64_07815"
     CDS             complement(5778..6314)
                     /locus_tag="VKN64_07815"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMA60185.1"
                     /translation="MNNAVWLIIPIILIRYLLLRSISEEAYQRASYFPPLEGKEQYAF
                     YIYQVALIFMLFYTIFLDIQTESAINILGFIIYIIGILSYIVSMYDFARPQADGLNNK
                     GIYSFSRNPMYVAFFLYFLGIALITKSVLYFIVLMVFQISVHYIILSEERACLTEFGD
                     EYKVYMQEVNRYLGRKFS"
     gene            complement(6329..7102)
                     /locus_tag="VKN64_07820"
     CDS             complement(6329..7102)
                     /locus_tag="VKN64_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA60186.1"
                     /translation="MQWFEIDKVDEDTYVISENKHWEEFNTYYLLGDRYNLVIDCGIG
                     IYKIKDLLNKIDQKEKKLIITHMHWDHIGNIEEFKEVYLSEKANYYFENGYWEPIEEV
                     RKKVIRDVNQNLLPDSFNIDEYEIEKSKRGKVIKEGETFGLGNRKIEVIETPGHTEDH
                     ICLYDKTNKYLFAGDFIYQGPLYLERDYTDIEDYFLSLNKLIENYPEIEKILSSHYKP
                     EISSKYLKEIYQFILKLKEKGEFKQGTGIHKNSKHEIIL"
     gene            complement(7137..7907)
                     /locus_tag="VKN64_07825"
     CDS             complement(7137..7907)
                     /locus_tag="VKN64_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HMA60187.1"
                     /translation="MEDTIKRLITDYVKEYPKKENTNTEWKEPLTAYADAQDPLFIKL
                     KEVISPNHELPTDLLDNAQSVITFFLPFKEEINESNIEGRLSSRQWAVAYIETNQLIM
                     AVNKYIQKELNKRGYNSTIIAATHNFREDQLISDWSHRHAAYIAGLGTFGINNMLITA
                     KGCAGRVGSIITDLKLKPTKRPQEEFCLYKYNGSCKVCLERCIVNALSENPAEFNRFD
                     CYEICLENEEYHKDLDLTDVCGKCCVNLPCTSQNPTKQ"
     gene            complement(7928..8287)
                     /locus_tag="VKN64_07830"
     CDS             complement(7928..8287)
                     /locus_tag="VKN64_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMA60188.1"
                     /translation="MNKINLQDKYNMIDEHWDPKIIGEVNDMYVKLAKIKGAFEFHTH
                     QDEDELFYIFKGQITMQYRDREEVIKEGEMVIVPRGVEHKPYAEKEAHIMLFESKETV
                     NTGDKENEFTKLEEDWI"
     gene            complement(8307..8465)
                     /locus_tag="VKN64_07835"
     CDS             complement(8307..8465)
                     /locus_tag="VKN64_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60189.1"
                     /translation="MKKLADIFSIGAQFINYCQDKGWIIEKGAGKNKEYYVSEEGVKE
                     LKKFNIEV"
     gene            complement(8515..8895)
                     /locus_tag="VKN64_07840"
     CDS             complement(8515..8895)
                     /locus_tag="VKN64_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60190.1"
                     /translation="MYIVLILLVIGLIFAYYSFKVTKKANAHSDKHPKGYYQSKGLAI
                     GLAFGYFGMFIILLIFDQMDYFVFLAPGAGLLLGVVLGYRMEKKHEDELRPLTEEELK
                     MKKYLKIFLLIVVVIGFIFFFLVR"
     gene            complement(8921..9481)
                     /gene="rpiA"
                     /locus_tag="VKN64_07845"
                     /pseudo
     CDS             complement(8921..9481)
                     /gene="rpiA"
                     /locus_tag="VKN64_07845"
                     /inference="COORDINATES: protein motif:HMM:NF017806.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
BASE COUNT         2814 a         1856 c         1261 g         3581 t
ORIGIN      
        1 aaatctatgt tctaaattgg ggatctttaa agtatattcc attattattt ttaatctttc
       61 tttgggcttt aaaatattat ctaattcaat tttaagcaag gttttatccg caccgataat
      121 ttcaaaatca attacttcac ccttaatttt tatttctgaa aattctatat aacctggctc
      181 ataatcggga tttacatttt caaccaaacc taatcctata atattttcac ggaaagcatt
      241 cggatataaa tgaaagtata cttgatttag atctgtattt tccttattaa tataatccac
      301 tgtctgttct attttgcata atctctgctc gggttcaaat ttaatatcaa tattataatt
      361 gtcaacttca tcaatattga ctccattata gtcttttgct attaggtcat cataatccct
      421 gtccagatag ttatatatac ttatattaaa aatattaaag gcagcaaaga ggagaacagc
      481 tactataaaa acaataatga tctttttcat ctttcttcac cttttcaaag aacttaatta
      541 aaaactgagt ttatttatca aaaggaactt ccttgttata tattttgttc agataatctc
      601 gcacctcatc ttccagagat tgaggatact caaagcggag cctataggct accttttttg
      661 aaattttact gaaaaagtcc atcattttaa aagtgctttc ccagttgtca ttaatatcac
      721 caccggaaaa tgttgctttt aattctttcc atagtttatc atccaaatat tttttaaagt
      781 tacttccctg tttaccgggg ttaacctgcc aattgttatc tactccgatc agccattcta
      841 tcatcggttt aagcaagttg tatttgataa agtagtccat gtacttggca aagtaaaatt
      901 ggtcacgcca gagatatttt gctatatagg tgatatccca ccagaagtta ttaactaccc
      961 tatcatacac ttcttgatta ggcttttttg tctgataggc attatgagta ggctcattta
     1021 attttactgt gatattattt tcatcatata aaacccggta accaatatcc aaaaaggccg
     1081 gcaatttatc ataattagaa acctttttta caagttctat cggccaaata ctaaagtcta
     1141 tcctgatata atcctgaaat atattgagtg aagtataggt tgtaatatct tctaacttaa
     1201 aattatcatt gaaagctacc atcacctcgc caaaatcatt aatccagctt ttatctttta
     1261 acaaactttt aggttctttg gtaactaaga caacatctag atcggaaaaa acatcggttt
     1321 ctaccctggg atttgccatc gaactcgtaa ttaaaagtgc ttctatatct gggttttgtt
     1381 tagcaaactt aataatatct tctaatactc tttctttatc tctcacttgg attatactcc
     1441 tttaatttaa gcttatatta tattttttga gtacccgtat agctcttaag gtatttattt
     1501 tactttccct tgtgttccct tctaatttaa agaattcttt gccgctgtgt ctgccctgga
     1561 ggggccattt acaggccttg tttttctttt ttaataataa ttgcagggca tcctccatcc
     1621 gttcatcata atctttatca taaaaataga aatattctaa gactcttaaa atatcataat
     1681 accagcgggg tgggaaagac agataggtga atttcttatc gattacctca cctgttttgt
     1741 cagacttata taatctatgc tgtaataaaa actcatgagc ttctttttcc atactgataa
     1801 cttcttcttt tttataatca aagacatttt tatactctaa aagaccttcc aggacattga
     1861 tagtagtgtg catcgaactg tgtttggctt ccttatgctt cttgcagttc cagcctccat
     1921 catgcatctg ttcggcaata aggtaatcaa ttaaattttt gattctttca tcttttacct
     1981 tgaaatagga aagtaaggaa agaactatgc cggtaataca agtttcactt ctcgtaagtg
     2041 ttttcgaaaa atttattccc ccatccttgt aaagacctct atccaataaa attacagtcc
     2101 ctcttctggc ctgcgaatta tcgcttggaa gggacaacct ttttaatagc agcagggtat
     2161 aagttgagga tatccatttg ggagagtata gtccaccacc ccaaatatca tcttcagctt
     2221 gttcatccaa cagtctcttt acccaaccct tagttaccat ttcatttctt aatacttgca
     2281 acttctgtgg ctcagtttct aaaatatcac gatagacctg atatctgata gctgggtcac
     2341 cttgtaacaa ccaataaact accctttgtt ctttcatctt ttcttctgcc tttcaattag
     2401 attagtcatt ctttttccct ttaatatcgt ctatttcaat ttctattatc tcagttttgt
     2461 cgattgcttt ttctatataa tcaaaacctt cctttttgaa atcaggagaa tattttaaaa
     2521 taaactgctg taaagctttt ttcttgattt caccatttaa ggaatttgct ctgccaaaaa
     2581 tcacgacact ttcatagtta gaagtgaatc cagccggttg aagttcataa tccccaacca
     2641 ctgtaaaaga aactttatta ttatagttaa tattctctat tttatgacct tcttttgcac
     2701 agtgaaggta aattttatca ttaaaaaaga cataattcat gggaagtgca tagggataat
     2761 cattttcacc tatggtggct aagaccccat actctccctt ttttagaatt tctaaagctt
     2821 ttactttggc caactgtttt tcctttcttc tcatctctat aaacatatta tcctccatat
     2881 catgtaaaat tttggtgctt taacattgct tgtttcgttt aaaaccatat ttttatcttt
     2941 tctttttacc gctgtataaa aagtaatctt catccttgat cttagtatct tcgaaattat
     3001 aggtccagct cattaatttt aaaacttcat cggtcgggat cggatcctct tgattatggc
     3061 ttatctcatc atgatgttct tttaactctt ttcttgtttt agaatgaatt attaaaaact
     3121 gtcctcctac ttttagatgt ttgcgggcat ttttaaatac catatcaatt ttatctaaat
     3181 ggggaatagt atcatagatt atgacaaaat caaatctctt atctaattca acattggttt
     3241 caaaatcgat tcttctaatt tcagcatcag gatgtttttc tttgaaaact tcaatcattt
     3301 taggagaaat atctatacct aaatatcctg ataagttttc atttttgagt agtgaataaa
     3361 gaatcccggt tccagctcct acatccagaa cagaattatt acggccgatc ttgtattttt
     3421 tgattacttc cttggctact tcaatatttt catcggtaat aaattcatcc cattcttcag
     3481 catgtttatt gaaaaactca atttcttctt ttttatcttt atccatactt ttgcccccta
     3541 ctaggattac tttctaaggt tttaatcatt ttctaaattc attaatattg tcttttgcat
     3601 gtgcagtaag ttttccttcg ccttataacc aataaagtaa tcagattcta taatctcctt
     3661 actaacctct tctcctcttg taaagggagg acagggatta actatacagt cattgctggc
     3721 ataacctatg ttttctaaat taatctgata ttgagcaaac ttgtcctgag ttttatatcc
     3781 atggggcggc caggcatctg ttaatattac atctgccccc cttattcctg tctgtaagtc
     3841 attagttctt tcaattattc cttcagaatt ttctgctgca aaattatata tatcatcttt
     3901 tacttcaaat ccttctggac aaacctgggt taaatttaaa tcaagtactc cggctgcatt
     3961 aaaccaggaa ttagatatat tggctccttc tcccacaaat acaaatttta agttgtttaa
     4021 attacccttt aattgatata gggtaaataa atcacttagt acttcacagg gatggtattt
     4081 tttagacatt gcattcacaa ctgaaaaatc agctaattta gctatttttt caattgtatc
     4141 ctgttcagga taccttatta tcaaatagtc aacccaatta tttaggtatt ttaccttgtc
     4201 tgcaagttct tccttaccct ctaggtccag ggataaattg attatatctc cccctaattg
     4261 gtttatcgca ctttcaaatg atatctttgt tcttaaactg gtggaaggaa aggtagtaat
     4321 tattgtttta tgtaagaggg gccttaagtc attaccatta tgatttttga tttttaatct
     4381 tttggtatcc gcaaacattt catatacttc gcttttagat agatctttta ttgataatag
     4441 atccttcatg gaattctcct ttttaaatta ttattaaaca ttaatcattt tttagggaaa
     4501 aactgacaat gagaacttga aacttctcta taattattct ccttctgcta tacttcaatt
     4561 ataaactctg ataaggcaaa agctgtatct aatacctttc gcttgtctat aatatccaaa
     4621 ttatcttctt ctgtatgaac cttgtctaag atttcttcta acaagtttga agtaaaagct
     4681 aatgctggta tgttcctttg aactaatatt gcatgttcac cctgatacca gggttctcct
     4741 ttcatcatac tatatttatt tttgaaaatt ttatcagata gattattgat attcttatca
     4801 gttgtataaa cggaaaaagc tgtgttacct ttcttatacc ccattccatc tatatttata
     4861 ccgaggtaaa tattttctaa cctgcttttg ttatctataa aatacacccg ttggcctgga
     4921 ttagaataat aatcttcccc gtttaataaa gcaacttcta cctgtttatc taaattttta
     4981 tcttttaata gttctattaa aagaagtaag gtggttacac ctgtagcgtt atcaatagca
     5041 ccaggtgaat caactttact gtcaaaatgg gcaaaaagaa ctattttatc cttgtttttc
     5101 tttccaactt ttgcaattac atttttaccc ttagcatcaa cctttgttgc atctattttc
     5161 agataaacat cttttcctac ctgctttttt atttcttctc ctgtcatatc tttgatataa
     5221 attgaaggaa ttaaaaaatc accatcttca attaaaggaa agggataaag acctccagct
     5281 gtgctttgat cttttccggt tgctgtaata acggctttaa aacctttttc ttctagtaat
     5341 ttaattatgg tctgatgttc ttcaacttga taaaaaggga aatttttagg cacaagttgc
     5401 tcttttacaa gctctccgga caataaaaca actttgtctt tcccctctaa tttttctaac
     5461 tgttctatat tagaggcagt cactaattct gctttaagtt caacaacggg agagtaggga
     5521 ctaatctggg cctcatattc ggtatcatca actttgaaaa ttaaatcatt gggataccag
     5581 tcaaaacaat caaaggtttg ttcttcaatt tcaacatctt ttaaattttc tttgatataa
     5641 cttgcagcct gttgattact cttactgccc acatatctat cacctaaatt attgcagaaa
     5701 tattccaggt gcttttctgt caaccttttt aaatctcttc tcaatacttt caccactctt
     5761 ttatatattt atctgtacta actgaatttt cttcccagat agcggttcac ctcttgcata
     5821 tatactttat attcatctcc aaattcagtc agacaggccc tttcttctga aaggattata
     5881 taatggactg agatctggaa taccattaat acaatgaagt atagaactga tttagttatt
     5941 aaggctatcc caagaaaata taaaaagaaa gcaacataca tggggttgcg cgagaaacta
     6001 tatattccct tattattcaa cccatcggcc tgaggtcggg caaaatcata catcgataca
     6061 atgtaagaga ggatgccaat aatatatata ataaagccaa gtatattgat agctgattct
     6121 gtttgaatat ctaaaaaaat tgtataaaaa agcatgaaaa ttagagcaac ttgatagata
     6181 taaaatgcat attgttcttt tccttccagg ggtgggaaat aagaggctct ctgataggct
     6241 tcttcactta ttgatcttaa taagagatat cttattaata ttattggtat aatcaaccag
     6301 actgcattat tcaatctgct ctactccttc ataagattat ttcatgtttg ctatttttat
     6361 gtatccccgt accctgttta aattcaccct tctctttcaa tttcaatata aactgataaa
     6421 tctcctttag atatttagaa gaaatttccg gtttataatg actggataat attttttcaa
     6481 tttcaggata attttctatg agcttattaa gagaaagaaa gtaatcttct atatctgtat
     6541 agtctcgttc cagataaaga ggcccctgat agataaaatc acctgcaaat agatatttgt
     6601 tggttttatc ataaagacag atatgatctt cagtatggcc tggggtttct ataacttcaa
     6661 tttttctgtt tcctaaccca aaggtttctc cctcttttat tactttacct ctttttgatt
     6721 tttcaatctc atattcatcg atattaaagc tatccgggag taaattttgg tttacatccc
     6781 taattacttt ttttcttact tcttctatcg gctcccagta accattttca aaataataat
     6841 ttgctttttc acttaggtat acctctttaa attcctctat attgccgata tggtcccagt
     6901 gcatgtgggt tataatcagt ttcttttctt tttgatcaat tttatttaat aggtccttga
     6961 ttttataaat tccaattcca cagtcaataa ctaaattata tctgtctcct aataaataat
     7021 aagtattaaa ttcttcccag tgtttgtttt cactgattac atatgtatct tcatcaactt
     7081 tatctatttc aaaccactgc atcaaatcac tccatcatct atttaaaatt aatcttttat
     7141 tgcttagtcg gattttggga agtacatggt aaattcacac agcacttccc acacacatca
     7201 gttaaatcca aatccttatg gtactcttca ttttccaaac agatctcata acaatcaaac
     7261 cgattaaatt cagctggatt ttcggataag gcattcacta tacatctttc taaacatact
     7321 ttacagcttc cgttgtattt atataaacaa aactcttcct gtggcctttt ggtcggcttc
     7381 aactttaaat cagttattat actgcccacc cggccggcac aacctttggc tgtaattaac
     7441 atattgttaa taccaaaggt acccaggccg gctatataag ctgcatgtcg gtgtgaccaa
     7501 tcacttatta actgatcctc cctaaagtta tgggtagcag caattatggt agaattatat
     7561 cccctcttat ttagttcttt ttggatatat ttgttaactg ccataatcag ttgattggtt
     7621 tctatatagg caaccgccca ctgtctggag cttaacctgc cttctatatt tgactcatta
     7681 atttcttcct taaatggtaa gaagaaggta atcacagatt gggcattatc taatagatca
     7741 gttggtaatt catgattcgg ggaaattacc tcttttaatt taataaatag aggatcctgt
     7801 gcatcagcat aagcagttaa aggttctttc cactcagtat ttgtattctc ttttttagga
     7861 tattctttca cataatcagt tattaatctt tttattgtat cttccatcag caacactcca
     7921 gaatatttta aatccaatct tcttctaact tggtgaactc attttcttta tctcccgtat
     7981 ttacggtttc ttttgattcg aacaacatga tatgtgcttc cttttctgca tatggtttat
     8041 gttctactcc ccttggtacg attaccattt ctccttcttt gattacctct tctctatccc
     8101 tatattgcat agttatctgg cctttaaaga tatagaaaag ttcatcttca tcttgatggg
     8161 tatgaaattc aaaggctcct ttaatttttg ctaatttcac atacatgtca tttacttccc
     8221 caatgatttt gggatcccag tgttcatcta tcatattata tttatcttgc aaattaattt
     8281 tgttcaattt aaacccctcc agctctttag acttcaatat taaacttctt aagctcttta
     8341 actccttctt ctgaaacgta atactcctta tttttaccgg ctcctttttc tattatccag
     8401 cctttgtcct gacagtaatt aataaactga gccccaatcg aaaaaatatc cgctaatttt
     8461 ttcatagcat tacccttctt tctaaaagtt aataaaaaca aattggtaaa tcagttatct
     8521 taccaggaag aagaaaataa atcctatcac tacaactatt aacaaaaata tctttaaata
     8581 ttttttcatt tttaattctt cttcggttaa gggacggagt tcatcttcat gttttttctc
     8641 catccgatag cctaatacaa ctcccaggag taaacctgcc cccggggcga gaaagacaaa
     8701 atagtccatt tgatcaaaga taagtaggat aataaacatc ccaaagtaac caaatgctag
     8761 tccaattgct aaaccttttg attgatagta tcctttcggg tgtttatcag aatgagcatt
     8821 agccttttta gtaaccttga aggaataata agcaaagatt aaaccgatga ccaataaaat
     8881 taacacaata tacataaaaa gtcacctgct tcctttaaga ctattctatt atctctacgg
     8941 aaccatcctc tttacctact actacctttt ctgccatatc tataaacagt ccagattcta
     9001 ctactcccgg cagaatgttt atagttgtat ttaattcggc tggattttca attacttcaa
     9061 agttacagtc gataatgaaa ttttcattat cggtattgaa aatttcatcc ccttctcttc
     9121 ttagacgagc ctgacaacct aatttttcaa ggttagttaa agccttttca cagccaaaat
     9181 gaactacttc taccggtaag ggaaattcac ccagggtttt cacttattta gattcatcta
     9241 ccactaatat caatcgctta ctttcatgag ctacgatctt ttctctaact aaagctcccc
     9301 cgccgccttt gatcaggttt aaatcggggt ctacttcatc ggccccatct attgttaaat
     9361 caatatgctt gacctcagat aaagtagtta aaggaatatc taattcttct gccaactgtc
     9421 tggtctcttc tgaagtaggt atagccctga tatttaaacc atcttttacc ctggctccga
     9481 tctctctagt tgcccaggct gctgtagaac ca
//
LOCUS       DATGCJ010000157         6185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_154477, whole genome shotgun sequence.
ACCESSION   DATGCJ010000157 DATGCJ010000000
VERSION     DATGCJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154477"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1332
                     /locus_tag="VKN64_07850"
     CDS             148..1332
                     /locus_tag="VKN64_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase-related protein"
                     /protein_id="HMA60191.1"
                     /translation="MKNSKSILFVSNGHGEDLIAANLIKSLLAQDNNLSISELPVVGK
                     GNTFEKMPVEILGPRETLPSGGFARNSWQNLWNDLKGGLISNTYKQIQVLRGMKNKVD
                     LSVVVGDVYILTLTGFFTGGDIVFLPTAKSEYINGHFKIEKHIMKRYADLVLPRDNKT
                     TNNLKSFGIKTKFFGNLMMDCFEIKGLDFNLRENSTKIGILPGSRDDAYDNMLDFINV
                     IEELERLKKEKYDFLTAVAGNFSVEKLKNKLKGSEWQALEQEDDYIKLQIISPSKASA
                     VSIIYNGFGDVLDQAKLFLGMAGTANEQAVGMGKPVVTFPGTGVQFDYEFAADQKRLL
                     GEGVKFVDRNFKNVAYALIELLEDEQEYNKRSKAGQKRMGDRGAIKKVSNLIFQDFLF
                     DI"
     gene            complement(1283..2473)
                     /locus_tag="VKN64_07855"
     CDS             complement(1283..2473)
                     /locus_tag="VKN64_07855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMA60192.1"
                     /translation="MSLINKITSINKMNLLLYIGWFCIALFFSWGNTSLGFLFLMIHS
                     IIYLIINRKEINFNLLFKFKTNIILISLLIWTLISAVFAYHKSLALASTLGFIILISI
                     TLFETKILINFKKFIYKFLLPIMTLGITIASSYIIYNYFSSNLRRATGVFSNVNDTGT
                     LLIISLILIISYFEYLNNNYKYLVLIPGLLNLLALLLTFSRGAFIGFIAGLAIYNLRS
                     KKHLVIFILIFILIFSFIYSNPKLNKRFIKSLTIEDNMDRINIWMSSLKMIKAHPIQG
                     VGPGNFPKVYPSYRWVEKKSNRYKSFAHNIFLNMAVETGIVGFILFTILIILVLSMGI
                     KLFNNNPIHRGIVAALIAVLIHNQFDCTVLKFEVGVVFWALIGLILAIYQKENPEKSN
                     WILF"
     gene            2788..3990
                     /locus_tag="VKN64_07860"
     CDS             2788..3990
                     /locus_tag="VKN64_07860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60193.1"
                     /translation="MFLWHGAFLALTMSMIDLNTVLPSLISELIDSKIIFGLLYSMIL
                     GVPRLFNIIFSHFLSYVKYKKKVLITGIYLRAFSFLGMALTTYYYGKDNPGTVIIFFF
                     IWIFMFSISGGFASLSYNEIIGKLTTKGQREKIFASKQFVASIMAFTGGLIVKYIFTP
                     GKYTFPNNYSILLSIGFLGLIIAAFSFWMIDEEPSTISEERLKLIKFLKRVPSLLKND
                     PKFLKFVLIENMSSFSLMVLPFYIVYAKDTFNLASEYVGTFLLFQIVGMITSNIFWGY
                     IGDKKGARYVVRVCILLGGIIPILALIAGQMNPGIYKYLFFLVGFVMSGRKIGFLPYL
                     LDIAPEDKRTVYLGIRDSLNILIIVLPLAGGVLINFLGYTLTFIIVSIVMLTAFFTDR
                     KDGCILLH"
     gene            4025..4312
                     /locus_tag="VKN64_07865"
     CDS             4025..4312
                     /locus_tag="VKN64_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HMA60194.1"
                     /translation="MRMNINILSGDDVIVKAQIVTLASLKAGESGTVYDFRGGERLRK
                     KLDSLGIRKGKNIKKVSDMVLRGPITIQINNSSRVAIGRGMAEKIMVKVGN"
     gene            4314..6119
                     /locus_tag="VKN64_07870"
     CDS             4314..6119
                     /locus_tag="VKN64_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HMA60195.1"
                     /translation="MKIILMGNPNVGKSVIFSRLTGTSVTSSNYAGTTVEYKKGLFKH
                     DGKNVELIDAPGTYTLDPTNKAEEVAVEMLKEGDVVFNIVDATNLERNLNLTLQLLEQ
                     DIPVVVLLNMWDETHHKGINIDLEKLRDELKVPVIPTVATTGNGLKKALDHLDYREVK
                     DFKKRTSEERWVEIGHIINDVQHIEPKHHSTKEIMEEYTLKPWTGIPIAVLVLYLTFM
                     VIRFIGEGLIDYIANPFFEGPYQRFLMEVSQFLTEGSFLHDLFIGQLVNGQIDFEQSF
                     GLLTTGIYIPFAAVLPYIISFYFVLSILEDSGYLPRLAVLVDNVMHKVGLHGFSIIPM
                     ILGFGCNVPAALAARNLDSKREQFIASTLMSISIPCMAQTAMIIGLVGAFGGIYVFFV
                     FAALFIVWIIVGLLLNYFLPGYSSDLLIEIPTLRLPLFDIVIKKLWMRITNFLKEAIP
                     FVLMGVLAVNALYALGFFDFLTRVIGPAFSVLFGLPKDVISALLMGFLRKDLAMGMLA
                     PLDLTVKQLVISSTILTVYFPCVATFIVLFRELGLRNMLKSAGVMVATAVTVGTILNI
                     GFNSIYDSVVTTILIFIIIFILYRLTSKKYERIME"
BASE COUNT         2117 a          864 c         1166 g         2038 t
ORIGIN      
        1 taataaatgc tgataagatt gtagttattg aagacggaga aattgtcgaa aagggaccac
       61 atcaagaact actggataaa gaaggctatt attataattt atataaaaaa caatttaaat
      121 aaattttaat taggattgat aaatattatg aaaaattcaa agagtatatt atttgtcagt
      181 aatggacatg gagaagattt gatcgccgct aatttaataa aatctctgct tgctcaagat
      241 aataatcttt caatatcaga actgcctgta gttggtaaag gaaatacctt tgaaaaaatg
      301 cctgttgaga tactgggccc tcgcgaaaca ttaccaagcg gaggttttgc ccgcaacagc
      361 tggcaaaact tatggaatga tctaaagggt ggtttaataa gtaatacata taaacagatt
      421 caagtgctga gagggatgaa aaacaaagta gatctttctg tggtagtggg tgatgtttat
      481 atcctgactt taacaggatt ttttaccggt ggagatatag tgtttttacc aactgccaaa
      541 tctgaatata ttaatggtca ttttaaaata gaaaaacata ttatgaaacg gtatgctgat
      601 ttagtattac cccgagataa taaaaccacc aataatttaa aaagttttgg tattaaaacg
      661 aagttctttg gtaatttgat gatggattgt tttgaaataa aaggacttga ttttaattta
      721 agagaaaata gtaccaaaat tggtatatta ccggggagta gagatgatgc ctatgataac
      781 atgttagatt ttataaatgt tatagaggaa cttgaaaggt taaaaaagga aaaatatgat
      841 tttctaacag cagtggcagg caacttctca gtagaaaaat taaaaaataa gttaaaaggt
      901 tctgagtggc aggcgcttga acaggaggat gattatatca aactgcaaat aatatcccct
      961 tctaaagcaa gtgcggtttc cataatctat aatgggtttg gtgatgtatt agaccaggct
     1021 aaattatttt tgggaatggc cggtaccgcc aatgaacagg cggtgggcat gggaaaacct
     1081 gttgtaacat ttccgggcac cggtgtccaa ttcgattatg agtttgcagc tgatcaaaaa
     1141 aggttattag gcgaaggagt caagtttgtt gaccggaact ttaaaaatgt tgcatatgcc
     1201 ttaattgaac ttttagagga tgaacaggaa tataataaga gaagcaaggc tgggcaaaaa
     1261 agaatgggag accgcggcgc aattaaaaaa gtatccaatt tgatttttca ggattttctt
     1321 tttgatatat agccaatatc agaccaatta aagcccaaaa aactactccc acttcaaatt
     1381 ttaggactgt acagtcaaat tgattatgga ttaagacagc tattaaagca gctactatcc
     1441 ctctatgaat tggattattg ttaaataatt taatgcccat acttaaaacc aatattatta
     1501 aaatagtaaa aagtataaat cctacgattc cggtttcgac tgccatattt aaaaatatat
     1561 tatgtgcaaa tgatttataa cgattacttt tcttttcaac ccatctataa gaaggataaa
     1621 ccttgggaaa attacccggg ccaactcctt gtattggatg ggcttttatc attttaagag
     1681 aggacatcca tatattgatt ctatccatat tatcttcgat agtaagagat ttaataaacc
     1741 tcttattgag ttttggatta gaatagataa aactaaagat aagtataaaa ataaggatga
     1801 atattactag atgtttcttt gagcgtaaat tgtaaattgc tagtcccgca ataaagccga
     1861 tgaaggctcc gcgagaaaaa gttaataaca gggcaagcaa attgagcaaa ccaggaataa
     1921 ggacgagata tttatagtta ttatttaagt actcgaaata agaaatgatt aaaatcaaag
     1981 aaattatcaa aagagtacca gtatcattaa catttgaaaa tactccggtt gctctcctga
     2041 gattagagct aaaatagtta tagattatgt atgaactcgc aatagttatg cctaaagtca
     2101 taataggcaa taaaaactta tagataaatt ttttaaaatt tattaatatt tttgtttcga
     2161 aaagggttat tgagatcaaa ataatgaatc ccagggtcga agctaatgct aatgatttat
     2221 ggtaagcaaa aactgcagaa attaaggtcc agatgagtaa agaaattaaa attatatttg
     2281 ttttaaactt aaataataag ttaaaattta tttcttttct atttataatc aagtaaatga
     2341 tagaatgtat cattagaaaa agaaatccta aagaagtatt accccaggaa aaaaataagg
     2401 cgatacaaaa ccaacctata tataataata aattcatttt attaattgaa gtaattttat
     2461 taattaaaga cataaattaa ccccgcttct caaattcttt tcaaacactc tatatattat
     2521 catataatag tgaattcatc aatttcaaat caaagttcga attaaataat ttgtgataac
     2581 tcatattaaa ttttttaact aaaacgatta tattattaaa tttacttttt aaattaaata
     2641 agagtataaa tgggattagc ttatttttag attttaaaaa tgacgtaacc attttacaga
     2701 ttttagaaaa gtattctgat aatatacata tgattaaaac ttaaggaggg aaacaaaatt
     2761 aaaataaata ataaaaaagt ttactttatt ttcctctggc atggggcctt tttagctctt
     2821 accatgtcaa tgattgattt aaatacagta ttaccctcat taatatcaga attaattgat
     2881 tcaaaaataa ttttcggcct cctttattcg atgatcctgg gagtaccgcg gctttttaat
     2941 ataattttta gtcatttttt aagttatgta aagtataaaa agaaagtatt aataaccggt
     3001 atttacttaa gggcattttc atttttggga atggccttga ccacctacta ttatggaaaa
     3061 gataatccag gcacagtaat tattttcttt tttatctgga tatttatgtt ttcgattagc
     3121 ggcggatttg cttcactttc ctataatgaa attattggaa agttgactac gaaaggacag
     3181 cgggaaaaaa tctttgcttc caaacagttt gtagcttcaa ttatggcctt taccggtgga
     3241 ttaattgtta aatatatttt cacccctggt aaatatactt ttccgaataa ctacagtatc
     3301 ttactttcaa ttggtttttt gggtttgatt atagcagctt tctcattttg gatgattgat
     3361 gaagaacctt ccaccataag tgaagaaagg ctaaaattga tcaagttttt aaaaagggtc
     3421 cccagtttat taaagaatga cccgaaattt ctaaaattcg ttctaataga aaacatgtcc
     3481 agttttagtt tgatggtgtt acccttttat atcgtatatg ccaaagatac ctttaatttg
     3541 gcaagtgaat atgtgggtac cttcctctta tttcagattg tagggatgat aacatccaat
     3601 attttctggg gatatattgg tgataaaaag ggcgctcgtt atgtagttag agtttgtata
     3661 ttattgggtg ggataatacc cattttagct ctaatagcag gacagatgaa ccccggtatc
     3721 tataaatatc tattcttttt agtcggcttt gtgatgagcg gacgcaaaat tggcttttta
     3781 ccatatttac tagatattgc cccggaagat aaaagaacag tatatttagg tattagagac
     3841 agtttgaata ttttaattat cgtcttacct ctagcggggg gagttttaat taacttttta
     3901 ggttatacct taacatttat catagtttcg attgtgatgt tgactgcctt ttttactgat
     3961 agaaaggatg gttgtatatt attgcactaa tctataagtt gtgttaagat agaggtgtgt
     4021 ccaaatgaga atgaatatca atatactttc aggagatgat gtaatagtga aagctcaaat
     4081 agttacttta gccagtctaa aggctggtga aagtggaacg gtatatgatt ttagaggggg
     4141 agaaagatta cgtaaaaagt tagactccct tggaataaga aaaggcaaaa atattaaaaa
     4201 agttagtgat atggtattaa gaggtccaat tacaattcag ataaataata gttcgcgggt
     4261 agccatcgga agaggtatgg cagaaaagat catggtaaag gttggtaatt aggatgaaaa
     4321 ttatcttaat gggcaatcct aatgtaggca aaagtgtaat attttctagg ttaaccggta
     4381 ccagtgttac ttcttctaat tatgccggta ccacagttga atataaaaaa gggttattta
     4441 aacatgatgg taagaacgtg gaattgatag acgcccccgg tacctatacc ttagatccta
     4501 ctaataaggc ggaagaagtt gcggtagaga tgttaaaaga aggggatgta gtattcaata
     4561 tagttgatgc cacgaactta gagcgaaacc tcaatttaac tttacagctc ttagaacagg
     4621 atataccggt tgtggtgctt ttaaatatgt gggatgaaac ccaccataag ggtatcaata
     4681 tcgatttaga aaaactaaga gatgaactca aagttcctgt gattccgacc gtagccacca
     4741 ccggcaacgg tcttaaaaaa gctttagatc atttggatta tagagaggtt aaggacttta
     4801 agaaaaggac atcagaagaa agatgggtag agatcggtca tattatcaat gatgtccagc
     4861 atattgaacc taaacaccac tctaccaaag aaatcatgga agaatatacc ttaaaaccct
     4921 ggactggtat tcccattgcg gtcctggtct tatatctaac ctttatggtg ataaggttta
     4981 taggggaagg gttaattgat tacatagcaa atcctttctt tgaagggcct tatcagcgat
     5041 ttttaatgga ggtcagtcag tttttaacgg aaggttcatt tttacatgat ctgtttatag
     5101 gtcaattggt aaatggacag attgattttg aacaatcttt cggactttta actaccggta
     5161 tttatattcc gtttgcagcg gtattaccat atattatttc tttttatttt gttttaagta
     5221 ttttagaaga ttccggttat ctgccgagat tagctgtatt ggtagataac gtaatgcata
     5281 aagttggttt gcacggtttt tcaataattc ctatgatttt agggttcggc tgtaatgtgc
     5341 cggccgcact tgccgcccgg aacttagaca gcaaaagaga gcagtttatc gccagtactt
     5401 taatgtctat ttcgataccc tgtatggcac agactgccat gatcataggt ttagtaggtg
     5461 cttttggcgg catttacgta ttttttgtct ttgctgccct gttcatagtc tggattattg
     5521 taggactttt attaaattat ttcctaccgg gttacagcag tgacctacta attgaaatac
     5581 caactctcag actgcctctt tttgatatag taatcaaaaa attatggatg aggattacta
     5641 atttcttaaa agaagcgatt ccttttgtat taatgggagt attagccgtt aatgcactct
     5701 atgcgctcgg tttctttgat tttttaacta gagttattgg cccggccttt tcagtcctct
     5761 ttgggctgcc caaagatgtt atttcagccc tgctgatggg ctttttaaga aaagatctgg
     5821 cgatggggat gttagcccca cttgatttaa cggtaaaaca gctggttatc tcaagtacta
     5881 tcttaaccgt atatttccct tgtgtggcaa catttatagt tctttttaga gaattaggtt
     5941 taaggaatat gttaaaatcg gccggagtta tggtagcaac tgctgttacg gtcggtacta
     6001 tattaaatat tggatttaat tccatttatg attcagttgt aaccacgatc ttaatattta
     6061 taatcatctt tatcttatat agattgacga gtaaaaaata tgaaaggatt atggaatgag
     6121 aaggaggata aaaaaatgta tacagttagt gaaggaaaga taaaattaga acacggatca
     6181 attaa
//
LOCUS       DATGCJ010000158         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_154578, whole genome shotgun sequence.
ACCESSION   DATGCJ010000158 DATGCJ010000000
VERSION     DATGCJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154578"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VKN64_07875"
     CDS             complement(<1..345)
                     /locus_tag="VKN64_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60196.1"
                     /translation="MGKIQTINGLVDAKDLGFTYTHEHLVCYPPEIAMKGDPDFELPH
                     EDKAIEELKMFKNAGGNTLVEGTAIDYGRSPKALKRIADAVDVNIVFLTGFNKGRFYP
                     NWVIKASIEELKE"
     gene            complement(345..1142)
                     /locus_tag="VKN64_07880"
     CDS             complement(345..1142)
                     /locus_tag="VKN64_07880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA60197.1"
                     /translation="MPGEIKIDLKDKVVVITGGTKGLGREIALGAAREGAKIAVCSRS
                     EDALEDLRNDLVEIYADQSNFYLQAVDVTEIKKLEDFASNIVDSLGKVDVLIANAGVT
                     DQKHHSLVDLPNETWEKIMKVNLNGSYFTIKTFLPLLEESKGNLIIMTSLLGQKGYGK
                     ANDGPYCTTKFGLEGLKEVAAEEYQGKVNVNTIFPAEKVNTNFFSHLSKEERAKLADS
                     EVILEPTLFLASLPEDSLTAHSLNAKKWREDSGYKEGLLKRARKENK"
     gene            complement(1126..2142)
                     /locus_tag="VKN64_07885"
     CDS             complement(1126..2142)
                     /locus_tag="VKN64_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60198.1"
                     /translation="MNLPIINKSMSRNAKVFISLLLVVVVLFAISGIFNPRSLSPNHI
                     LEVVRQSAPLGITAMGQSAVILTGGIDLSVGEIITMTNIISADMMKGRNLRTVPVVLL
                     LLLIGALIGGMNGFMIATTNIPPMVMTFAMSSMVKGGYLLYSGGAPRGRVSPILRYIG
                     TERFLGIPVTIIVYALLIVAVLYYFQKTKWGRSAYFIGNNPVAAEYAGIPKTYRLIMI
                     YAFSAMMAVIAGLILSGYIGIGNFDVGGDSYMLNSIASSVVGGNTFNGIGNVGGAAIG
                     ALIITVIESVMTSLGVAETGKLVMRGIIIITMVTLYMGSIDLGKVKKLFSGEGGSLED
                     ARGN"
     gene            complement(2156..>2682)
                     /locus_tag="VKN64_07890"
     CDS             complement(2156..>2682)
                     /locus_tag="VKN64_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60199.1"
                     /translation="LSHPGGFCPRMFTNAWLTELGGIVPLSIIYFFILILFTYFILNH
                     TRFGRRVYAIGGDEEKAKIAGINVDKVKIKVYMASSLLAGIAGLALAARMRSGDPVSG
                     STFTLYSVAAVLIGGTTFSGGKGGVIRTFAGVLILSLVSIILNLIGVSPFYQNVMTGL
                     IIVLAVINSSLRKG"
BASE COUNT          901 a          598 c          361 g          822 t
ORIGIN      
        1 ttctttcaat tcttctatac tggcttttat cacccagttt ggataaaatc tccctttatt
       61 aaaaccggta agaaaaacaa tattaacatc aacagcatca gctattcttt ttaaagcttt
      121 aggagatcta ccataatcta tagctgtacc ttctactaaa gtatttcctc ctgcattttt
      181 aaacattttc aactcctcaa tagccttatc ttcatgagga agttcaaaat ctggatcacc
      241 tttcattgct atttcaggag gataacagac taaatgttca tgagtatagg taaaacctaa
      301 atcttttgca tctaccagac cattaatagt ttgtatcttt cccattattt attctccttt
      361 cttgctcttt ttaataaacc ttctttatat cctgaatctt cccgccactt tttggcattt
      421 aaagaatgag cagttaaact atcttcagga agtgaagcca aaaataaagt aggttccaga
      481 ataacttccg aatcagccaa tttagctctc tcctcttttg ataaatgaga aaagaaattt
      541 gtgttaacct tttctgctgg aaaaattgta ttaacattta cctttccctg atattcttca
      601 gctgccactt cctttagacc ttcaaggcca aatttagtag tacagtaagg tccatcattg
      661 gccttcccat aacctttctg gcctaaaaga gaagtcataa taattaaatt ccctttactt
      721 tcttccaaaa gtggtaaaaa agttttgatt gtaaaataac tgccatttaa attcaccttc
      781 atgatcttct cccaggtttc attgggcaga tcaaccagag agtggtgttt ttgatctgtc
      841 actccagcat tagcaattaa aacatcaact tttcccaaag aatcaactat attagaagca
      901 aaatcttcca gcttcttaat ttcagttaca tctacagcct gtagataaaa attgctctga
      961 tcagcataaa tttctactaa atcatttctt aaatcctcaa gagcatcttc actccgggaa
     1021 cagactgcaa ttttggcacc ttcccgagct gctcccaggg cgatttcacg ccctaatcct
     1081 ttggttccac ctgtaatcac cacaacttta tccttaagat caatcttaat ttccccgggc
     1141 atcctcaagt gaaccccctt ctccactaaa gagtttttta acttttccta aatcaattga
     1201 acccatataa agagttacca tggtaataat aatgatacct ctcattacca gtttaccggt
     1261 ttcagctact cctagagaag tcattacact ttcaataaca gtaataatca aagcaccaat
     1321 tgctgctcca cctacattac cgataccatt aaaagtatta cctccgacaa ctgaggaagc
     1381 tattgaattt aacatatagg aatctccacc aacatcaaaa ttaccaattc caatatatcc
     1441 agaaagtatt aagcccgcaa tcacagccat catcgcacta aaggcataaa tcataattaa
     1501 tctatatgtt ttaggtatac cggcatattc tgctgccacc ggattgttac cgataaaata
     1561 agcagatctt ccccatttag ttttctggaa ataatagagt acagctacaa ttaagagagc
     1621 ataaactatg attgttactg gaattcccag aaatctctca gtaccaatat atcgtaaaat
     1681 tggtgaaacc cgacctcggg gagcaccacc actataaagt aaataacctc ctttaaccat
     1741 tgaagacatg gcaaaagtca taaccattgg tggtatatta gtagtagcaa tcataaaccc
     1801 gttcattcca cctatcaaag caccaataag caaaagtaat aaaactacgg ggacagttct
     1861 taaattacgc cctttcatca tatcagcaga aataatatta gtcatggtaa tgatctcacc
     1921 tactgagaga tctatccctc cggttaaaat aactgctgat tgtcccatgg cagttattcc
     1981 cagaggagca ctctgtctta ctacttctaa tatatgattt gggctaagac tgcgtggatt
     2041 aaaaattcca cttattgcaa ataaaacaac tacaactaat agcaatgata taaagacttt
     2101 tgcattacga ctcattgatt tatttattat tggtaaattc atttcagatc acctcttaac
     2161 cctttctcag tgaactatta ataacagcca gtactataat taaaccggtc atcacatttt
     2221 gataaaaagg agatacacca attaaattta aaataatact aaccagactc aaaattaaaa
     2281 caccggcaaa agtccttata actccacctt taccaccact aaaagtagtt cccccgatta
     2341 aaacagcagc aactgaataa agggtaaagg ttgaaccact aaccggatca cctgatctca
     2401 tccgggccgc aagagccaga ccggctatac ctgctaataa agaactggcc atataaactt
     2461 ttatttttac tttatcaaca ttaatcccgg ctattttggc cttttcttca tctccaccaa
     2521 tagcataaac tctccgacca aaacgggtat ggtttaaaat aaaataagtg aataaaatta
     2581 atatgaaaaa atatattata gataagggta ctattccacc taattcagtt aaccaggcat
     2641 tagtaaacat tctcggacag aaaccacctg gatgggataa aa
//
LOCUS       DATGCJ010000159         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_65746, whole genome shotgun sequence.
ACCESSION   DATGCJ010000159 DATGCJ010000000
VERSION     DATGCJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65746"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="rffA"
                     /locus_tag="VKN64_07895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             <1..1080
                     /gene="rffA"
                     /locus_tag="VKN64_07895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HMA60200.1"
                     /translation="KYHTRGDGYYTEKVSALLEEKFSVYKVFMTTSGTHALEMSTSLI
                     DLKSGEEVIMPSFTFPSTANAVLKNGGIPVFADIKPDTLNIDPKDILDKITNQTKAIM
                     PIHYAGIAADMEKIKEIAAEFNLYIIEDAAQAVNSQYKGNYLGTIGDFGCFSFHSTKN
                     YISGEGGALLINKEEEELVKKAAVLREKGTNRFSFLKGEVDKYTWVEEGSSYLPSDLL
                     MAFLYAQLEKMDQIKNMRKNIFDIYQNKLKNINNYHFVNSITKIPKGRKINYNSFYII
                     FNDKNIRDKMIELLKEKGITATFHYIPLHISRMGDKLGYKKGNLPVTEKTADTILRLP
                     LYTQMSERKVDYVINNIKEIFEELK"
     gene            1080..1829
                     /locus_tag="VKN64_07900"
     CDS             1080..1829
                     /locus_tag="VKN64_07900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMA60201.1"
                     /translation="MQETISIVIPVYNSYNSLEELYNKLTKTLEKNFDNYELIMVDDH
                     SQDESFKKLKELHEKDNRIKVIKLAYNFGQQNALMCGFHHSSGDYIVTMDDDLQHLPE
                     DVIELYQEIKKGYDIVYGIPAERQHLFYRRIGSYLTDKIFNLITNKSKNIRVSSFRIL
                     KRSLLNKVIKDKRSFVYLSAIILDSTDNIGNIYIEHEDRKHGDSNYNFFKLFKLFAKL
                     YIYYSNNKLLRIFRKTNPQYIIEEKMGFTKE"
     gene            1836..>2751
                     /locus_tag="VKN64_07905"
     CDS             1836..>2751
                     /locus_tag="VKN64_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMA60202.1"
                     /translation="MKLLILGGGNAQLSAIRKAKDMGHEVIISDYLEDAPGIKLADHH
                     ELVSTFDVEANIKVGKKYNIDGIMTIGTDQPVYTAAAVAEALSIPTLLDKETAKAVTN
                     KRVMKRRFKENNIPTVNYQLIKKDFDFEDIKEINFPAVLKPLDSQGQRGVFKINSVDK
                     IDQYFSKVVKYSRESEILLEEYYDSDEITVSGWVDKGKLHILSITDRVTYEQDVNIGI
                     CTAHMFPSKHLEKHHDEVEEISNKIIKAFNINNGPIYFQMLVGEAGIKVNEIACRIGG
                     AYEADFMPLITDVDILKMVINSSLGKSIN"
BASE COUNT         1032 a          373 c          506 g          840 t
ORIGIN      
        1 aaatatcata ccaggggtga tggttactat acagaaaagg tgagtgcttt attagaagaa
       61 aaattttctg tttataaggt atttatgact acatccggga cccatgctct agaaatgtcc
      121 acttctttaa tcgatttaaa atcaggagag gaagttatta tgccttcttt taccttcccc
      181 tcaactgcca atgctgtctt aaaaaatggc ggtataccgg tctttgcgga tatcaagccc
      241 gatactttaa atattgatcc taaagatata ttagataaga taactaacca gacaaaagca
      301 attatgccca ttcattatgc cggtattgca gctgacatgg agaagataaa agagatagca
      361 gccgagttta acctatatat aattgaagat gcagcacaag cggttaattc ccaatacaaa
      421 ggtaattacc tgggtacaat tggtgatttt ggctgtttta gctttcacag tacaaaaaac
      481 tatataagcg gagaaggcgg cgctttattg atcaataaag aggaggaaga attggtcaaa
      541 aaagccgctg ttcttagaga aaaaggtact aaccgtttta gttttttaaa gggggaagtt
      601 gataaatata cctgggtaga agagggttca agctatctgc catcagattt gttgatggca
      661 tttttatatg cccaactaga aaaaatggat caaataaaaa atatgagaaa gaatattttt
      721 gatatatatc aaaacaaact taaaaatata aataattatc attttgtgaa ttcaattacc
      781 aaaataccta aaggacgtaa gatcaattat aattcttttt atattatctt taatgataaa
      841 aacatcagag ataaaatgat cgagttatta aaagaaaaag ggataacagc tacttttcat
      901 tatattcctc tgcatatttc tcgaatggga gataaactag ggtataaaaa aggaaactta
      961 ccagtgactg aaaaaactgc agatactata ctacgtttac ctctttatac tcaaatgagc
     1021 gaaagaaaag tagattatgt cataaataat atcaaagaaa tctttgagga gttaaaataa
     1081 tgcaggagac catttcaatt gtgattccgg tttataacag ctataattct ttggaagaac
     1141 tttataataa acttactaaa actcttgaaa aaaattttga taattatgaa ttaataatgg
     1201 ttgatgatca tagtcaggat gagagtttta aaaagttaaa agaacttcat gaaaaagata
     1261 atagaattaa ggtaatcaag ttggcttata actttggaca acagaatgca ttgatgtgcg
     1321 gttttcatca ttcatcagga gattatatcg taactatgga tgatgaccta cagcatctgc
     1381 cggaagatgt aattgaactt tatcaagaaa ttaaaaaagg ttatgatatt gtatatggta
     1441 tacctgctga aagacagcat cttttttatc gaagaattgg ctcctattta acagataaaa
     1501 tatttaatct gataaccaat aaaagcaaaa atattagagt cagtagtttt agaatactaa
     1561 aaagaagctt attaaataag gtaataaagg ataagagatc ttttgtttat ctttccgcta
     1621 tcattttaga ttcaactgat aatatcggta atatttacat agaacatgaa gacaggaaac
     1681 atggagattc taattataat ttctttaagt tattcaaatt atttgctaaa ttatatattt
     1741 attattctaa taataagtta cttaggatat ttagaaaaac taatcctcag tatattatcg
     1801 aagagaaaat gggatttaca aaggagtaaa aatagatgaa gttactaatt ttagggggag
     1861 gcaatgccca attaagtgcc attagaaaag caaaagatat gggacatgaa gttattatct
     1921 ccgattattt agaagatgct cctggcatca aactagcaga tcaccatgaa cttgttagta
     1981 cttttgacgt ggaagccaat atcaaggtag gaaaaaagta taatatagat ggtataatga
     2041 caatcggtac tgatcaacct gtttatacgg ctgctgctgt ggcggaagct ttatcaattc
     2101 ctaccctgtt agacaaagaa acagccaaag cggtgaccaa taaaagagtg atgaaaagaa
     2161 ggtttaagga aaataatata ccaacagtca attatcagtt aataaaaaaa gattttgatt
     2221 tcgaagatat caaagaaatt aacttcccgg ctgtacttaa accactcgac agccagggac
     2281 agcggggagt atttaaaatt aattcggttg ataaaataga tcagtatttc tccaaggtag
     2341 tgaaatattc tagggagtct gaaatcttat tagaagagta ttatgatagt gatgaaatca
     2401 cagtaagtgg ttgggtagat aaaggaaagc tccatatttt atccattact gaccgtgtta
     2461 cttatgagca ggatgtgaat atagggatct gtactgccca tatgttccct tctaagcatc
     2521 ttgaaaaaca ccacgatgaa gtagaagaga tttcaaataa aattattaag gcttttaata
     2581 ttaataatgg gccgatctat tttcaaatgc tagttggaga ggcaggtatt aaagtcaatg
     2641 aaattgcctg tcgcatcggg ggagcttatg aagcagactt tatgccctta attactgatg
     2701 tagatatatt aaagatggtt attaacagtt ctttaggtaa aagtattaat a
//
LOCUS       DATGCJ010000160         6219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_112593, whole genome shotgun sequence.
ACCESSION   DATGCJ010000160 DATGCJ010000000
VERSION     DATGCJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112593"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1246)
                     /locus_tag="VKN64_07910"
     CDS             complement(32..1246)
                     /locus_tag="VKN64_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60203.1"
                     /translation="MWFEFKVAWRFLKSGKTQTILILLGIIIGVAVQVFLGSLISGLQ
                     QDLINQTVGSSPHITILPEDQRPRPLVVNEDKYSRIITFAGTQSGINRWREIEGYLND
                     VDEITSVSPTIDGSGFAVRGQKNLPVVLRGVEINKADGIYNIEENIVNGQTDIGGNNI
                     LIGTELANELELEPGDTLKINTSGGVTDRFIISGIFDLGSKELNSSWIFLSIRRAATL
                     LNFSDEISNIEVQIQDVFTANAISDQIMNRFANINAESWQRNNESLLTALQSQSSSSL
                     IIQVFVIIAVTLGIASVLAVSVVQKSRQIGILKAMGTKRKRVGIIFLIQGAILGLTGS
                     ILGALTGVGLSQAFVTFARGESGDALFPITIDPTFILISISIATAAGMIAASIPARNS
                     AKLNPIEVIHNA"
     gene            complement(1252..2568)
                     /locus_tag="VKN64_07915"
     CDS             complement(1252..2568)
                     /locus_tag="VKN64_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMA60204.1"
                     /translation="MKKSYIILIIIILAAAGGWFYNQNLNQPEMTEYVTVQRTELIES
                     LRASGVVKPHKTIEISSTIAENVKTINFEEGDHVKKENLLLKFDNDIAQAEYEQALAQ
                     VKSAEASLEQTEAQIQESKAQIDLAETKLENATELNDESIKEEIAQAELEIQNAVSEL
                     ERRQYLYENDAIEKIKVEQQQHTVNVLESRKKVLEQRLVELEKTRNNRVKEAEEELKR
                     AEVAYQAAQKQYQVAEGNLNSAKAALNRALVNLNKYDIKAPIDGVILKKMVDEGEYVQ
                     PGRTLFEIGTEELQIEISPDEKELNLLSIGKKGYVSPEAFPNSRYEVEIFEIAPSIDS
                     DRGTIDVYLKPTEENETLIPNMSVSVEIISEREEDVLLIPRNYVLEDSNGKYLYLYNS
                     ETAQRQSIEVDKNYNGQVIVTEGVKEGDIVLDPNNITDGQTVVIEE"
     gene            complement(2818..3024)
                     /locus_tag="VKN64_07920"
     CDS             complement(2818..3024)
                     /locus_tag="VKN64_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60205.1"
                     /translation="MNQIKLNYDNQISFKNILLNKLKISKNLFINKKTHSTLYQNKKK
                     KLDDYLGILNKNISSDELVNKLRD"
     gene            complement(3072..3713)
                     /locus_tag="VKN64_07925"
     CDS             complement(3072..3713)
                     /locus_tag="VKN64_07925"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HMA60206.1"
                     /translation="MLLHKERQLITEYGKKLIEKGLTKGTGGNLSIYNQKEGLIAISP
                     SGIPYEEIKLKDIVIIDLEENIVEGDHKPSSEKELHRLLYQKREDINSIIHTHPIYST
                     TIACLNQEIPAVHYLVAYSGDKVPCAKYATFGSKKLAKNVYKTMGKEYKACLMTNHGL
                     ITVGYNIKEALEISTMIEFTAELYYRTKAMGEPKILNKKQMNQVMNKFDDYRN"
     gene            complement(4110..>6219)
                     /locus_tag="VKN64_07930"
     CDS             complement(4110..>6219)
                     /locus_tag="VKN64_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60207.1"
                     /translation="HNQQKGSDRFKPIENIKEKRGYKLLESLSNIELISVKDDLVKVK
                     RKITQALSKRCTRNTYTRLQDVPVCECGFQLGDKVNLPSKKEIQKIIDNGVRQYINKL
                     KTLEYKEKIAVYLDNMEAAGNKRFARPIRNLINVKIDEDIYNKLDDILNRNVIKRINT
                     ALSGDVSLVERDLDDLYENLINRSFSPTQIREIFKDWLEGSSGVGEKTYIKVKGNLDQ
                     RLQQTESKEKGEVLKNYINQNYPELNLLIQNTDINVFIKLFAVVAWREIYKIDNSLDN
                     LITEIIPENSTVLMEELKRNTGIEASLQEMYEDLYTEQANLDVKKEISKKVDDLDLSR
                     PLMDIISPANIKEVIEAIKSEKLSEKLINKILIQFVNIIENIHLDYQEKQALKKEVEE
                     INEKINQGPLSEYKKTSLAVIANYLSLEKSFSYLDSKENINDIKGWKDVYVNHLADLE
                     YNYFKLDRLSKQTNIEDRIPKSIKYKKVKQTINNYQKLFTNFHDSRDIMGEDYERFMG
                     NNLIYLLKDKYPSLIKKMSVNKGYCLLLDGMRWDLWKLIKKQISEELALRIIDENSLF
                     ALNPTNTETQLEALNKAGLEINILEQNKVLNKLNSLNNNTEKCEEIIRFSYIDNKVHS
                     FKEDYISFIEEIQFQTRNRLIPFLEKVESKSGILIFSDHGYKINHNFKQKEKYEEPRY
                     LHGGKTFHEIIVPWAFIYKP"
BASE COUNT         1698 a         1136 c          951 g         2434 t
ORIGIN      
        1 tacttttatt aagtcttttg tttctatcat attaggcatt atggatcacc tcaatcggat
       61 ttagtttagc tgaattacgg gctgggattg aagctgcaat cataccagct gcggttgcaa
      121 tagatattga gattaaaata aaggtcggat caattgttat cgggaacagg gcatctcccg
      181 attctcccct tgcaaaagtt acaaaagcct gacttaaacc aacacctgtt aaagcaccca
      241 ggatagaacc ggttagacca agaattgccc cctggataag gaagataata ccgactcttt
      301 ttcgtttagt tcccatcgct tttaaaatac cgatctgtct tgacttctgg acaactgaaa
      361 ctgccaacac actggcaatt cccagagtta ctgcaatgat tacaaagacc tgtattatca
      421 aactggaaga ggattgactc tgtaaagcgg ttaaaagaga ttcattattc ctctgccaac
      481 tttctgcatt aatattagca aatcggttca tgatttggtc tgaaatagcg ttagctgtaa
      541 aaacatcctg gatctgtact tcaatattgg aaatttcatc actaaagtta agcagggtag
      601 ctgcccttct gattgataaa aagatccagc ttgaatttag ttctttggaa ccaagatcga
      661 atattccact gataataaat ctatcagtaa ctcccccgga agtattaatt tttaaagtat
      721 ctcccggttc taattctaat tcatttgcca gttcagtacc gattaaaatg ttattgcccc
      781 cgatatcggt ctgtccattc actatatttt cctctatatt atatattcca tcggctttat
      841 taatttctac tcccctaaga acaacaggaa ggttcttttg accccgaaca gcaaaacctg
      901 aaccgtctat ggtaggcgat actgaggtta tttcatcaac atcatttaga taaccttcta
      961 tttcacgcca tctgttaatc ccactttgag taccggcaaa agtaattatg cgggaatatt
     1021 tatcttcatt aaccactaac ggccgaggtc tttggtcctc cggtaatatt gtaatatggg
     1081 gtgaactgcc cacagtctga ttgattaaat cctgctgtaa gccgctgatt aaggagccta
     1141 agaatacctg gactgctact cctatgatta ttcctaaaag tattaagatt gtctgggttt
     1201 tacctgactt taagaaacgc caggcaactt taaattcaaa ccacatctaa atcactcctc
     1261 aatcactaca gtttggccgt cagtaatatt gtttggatcg agtactatat ccccttcttt
     1321 tactccctct gttacaatta cttggccatt ataattttta tcaacttcga ttgactgtct
     1381 ttgggcagtt tcactgttat ataaatataa gtactttcca ttactgtctt ctaatacgta
     1441 atttctgggg atcaataata catcttcctc tcgttcgctt atgatttcaa ctgaaactga
     1501 catattgggt attaatgttt cattttcttc cgtcggtttg agatagacat cgattgtacc
     1561 ccgatcagag tcaatactgg gtgcgatttc aaaaatttct acctcatatc tgctattagg
     1621 aaatgcttcc ggagaaacat aacctttttt acctatggaa agtaagttta attctttttc
     1681 atcgggactt atttcaatct gtagctcttc agttccgatt tcaaatagtg ttctgcccgg
     1741 ttgtacgtat tcaccttcat ctaccatctt ctttagtatc acaccatcaa tcggtgcctt
     1801 aatatcatat ttatttaaat taactaatgc cctatttaaa gctgctttgg ctgagtttaa
     1861 gttgccttct gctacctgat actgtttttg ggcggcctga taggcaactt cagctctttt
     1921 taattcttcc tctgcttctt taacccggtt gttacgggtt ttctccaatt caactagcct
     1981 ttgttcaagt acctttttcc gtgattccaa aacattaact gtatgctgtt gttgttctac
     2041 ttttatcttt tcgatcgcat cgttttcgta aagatactgc cttctttcta attcactgac
     2101 agcattttga atttctaatt cagcctgggc tatctcttcc tttatggatt catcatttaa
     2161 ttcagttgcg ttttctaatt tagtttctgc tagatcaatc tgtgctttag attcctggat
     2221 ttgtgcttcg gtttgttcta aagaagcttc agcactttta acctgagcta aggcctgttc
     2281 atattcagcc tgggctatat cattatcgaa tttaagtagt aagttttctt ttttaacatg
     2341 atcgccttct tcaaaattta ttgtcttaac attttccgcg attgtagaac taatttcaat
     2401 tgttttatga ggctttacaa ccccggaagc ccttaaggac tcaattagtt ctgttctttg
     2461 taccgtaaca tattctgtca tttccggctg attaaggttt tgattataaa accaaccccc
     2521 tgctgcagct aatataatta tgatcaatat aatatagctt tttttcatga ttaccctcct
     2581 tgcaaatttg atgaaaataa attactaaaa aaatatataa atttgaaatt atttattatt
     2641 ttcatctttt attcatcttt atttactata ttatatcaca taacgtgatt aatatataaa
     2701 atatattaca atttatgata attttttatc ttttttttag aaaaataaaa aactcccgat
     2761 taagggagtt taatttgttc ataattagat tatatttaaa atattcttaa ttaattttta
     2821 atcgcgcaat ttattcacta actcatcaga actaatgttc ttatttaaaa ttccaagata
     2881 gtcatctaat ttcttcttct tattttggta gagggtagaa tgggtctttt tattaataaa
     2941 aagattttta ctgattttta atttattaag tagtatattt ttgaaactga tctggttgtc
     3001 ataattaagt tttatttgat tcaccctttt ctcctcccaa cttattttgt gaacaatttt
     3061 aaattttaac tttaatttct ataatcatca aacttattca ttacttgatt catttgtttt
     3121 ttgtttaata ttttaggttc ccccatagct tttgttctgt aatataattc agctgtaaat
     3181 tctatcatag ttgaaatctc caatgcttct tttatattat atcctactgt aattaagccg
     3241 tggttggtca ttaaacaggc tttatattct ttccccatcg ttttatagac atttttagcc
     3301 aattttttac tgccgaaggt agcatactta gcacagggca ctttatcacc agaataggct
     3361 actaaataat ggaccgccgg tatttcttga tttagacatg ctatagtcgt tgaataaatg
     3421 ggatgggtat gtataattga gtttatatct tctctttttt ggtataatag gcggtgtagt
     3481 tctttttctg aagatggttt atgatcccct tcaacaatat tttcctctaa atctattatc
     3541 acgatatctt ttaatttaat ttcttcataa ggtattccag aaggtgagat ggcaattaag
     3601 ccttcttttt gattataaat acttaaatta ccgcccgtac ctttggttaa tcctttttca
     3661 attaactttt taccatattc agttattaac tgtctttctt tatgtaataa catgtgtacc
     3721 attcctttga attattctca cttcacttct atctttaata aaccgatctg ttgtttatta
     3781 cgggcaaact cctgaacttt ttctgagaaa aagtcggctg ccagccagat tgcgtggtag
     3841 ttaaggaagt tgtcttttat gaggtcaata agttcttcta gattatcttc tgtgacttct
     3901 tttactaaaa cataaatatg gacccgctta aagatgaaaa atgtcttctt tttggtcctt
     3961 acacaataaa gctcatcctt atattctaaa tcttgggttt tagccttcca gccgtcggtt
     4021 gttaaatttt tgattaattc ctgttcatgt tcttttaaag ttttaatttt ttttcacctt
     4081 cttatcaaaa atattattat ataattgctt tatggtttat agataaaggc ccagggtact
     4141 atgatctcat gaaaagtttt tccaccgtgt agataacggg gctcttcata tttctctttc
     4201 tgcttaaagt tatggtttat tttatagccg tgatcggaaa agattaggat accgcttttt
     4261 gattcaacct tttctaaaaa tggtatgaga cggtttctgg tctggaactg tatttcttcg
     4321 ataaagctaa tataatcttc tttgaaactg tgtactttat tatctatata ggaaaaccta
     4381 atgatttctt cacatttttc tgtgttatta ttaagggaat tcaatttgtt taataccttg
     4441 ttttgttcta gaatatttat ttctaagccg gctttgttta gagcctctaa ttgtgtttca
     4501 gtattagttg gattgagggc aaacaaagaa ttttcatcaa taattcttaa ggcaagttcc
     4561 tcagagatct gtttttttat caacttccag aggtcccatc tcatcccatc taagagtaga
     4621 cagtaaccct tgttcacact cattttcttt attaaagagg gatatttatc ctttaagaga
     4681 tagattaagt tattacccat gaacctttca tagtcttctc ccatgatatc tcggctgtca
     4741 tgaaagttag taaatagttt ctggtaatta ttaatggtct gttttacctt tttatatttt
     4801 attgatttag ggatcctatc ctcaatatta gtttgcttgg atagtctatc taatttgaag
     4861 taattatact ctaaatcagc taagtggttt acatagacat ctttccagcc tttgatatca
     4921 tttatgttct cttttgaatc gaggtaacta aagctttttt ctaatgacag gtaattagct
     4981 ataactgcga gagatgtttt tttgtattca gatagtggtc cctgattgat tttttcatta
     5041 atctcttcta cttctttttt taatgcttgc ttttcttgat agtccaggtg gatattttct
     5101 atgatattca caaattgaat taagatctta ttgatcaatt tctcagataa tttttctgat
     5161 tttatggcct ctattacctc tttgatattg gccggtgaga taatatccat taatggtctg
     5221 gatagatcta gatcatcaac ctttttagaa atttcttttt taacatcaag attggcttgt
     5281 tcagtataga ggtcttcata catttcttgt aaggaggctt caatgcctgt atttctcttt
     5341 aattcctcca ttaagaccgt actattttca ggaataattt cggttattaa attatccagg
     5401 gaattatcta ttttatagat ttcccgccag gcaactacgg caaagagttt tataaagaca
     5461 ttaatatcgg tgttttggat aagtaggttt agttcgggat aattttggtt gatatagttt
     5521 tttaatactt ctcctttttc cttagactca gtttgttgta atctttggtc caggttacct
     5581 ttaaccttaa tataggtttt ttctcctacc ccactgctgc cttccagcca atctttgaat
     5641 atctctctaa tctgggtggg agaaaagctc ctgtttatca agttctcata gaggtcatct
     5701 agatctcttt ctaccaggga aacatcaccg gataaggcag tattaatcct tttaattaca
     5761 tttctattta agatatcatc taatttatta tagatatctt catcaatttt aacattgatc
     5821 aggtttctaa tcggacgagc aaagcgttta ttgccggccg cttccatatt atcaagataa
     5881 actgcgattt tttctttata ttcaagtgtt tttaacttgt tgatatattg tctaactccg
     5941 ttatcgatga tcttttggat ctcttttttg gatggtagat taactttatc acccagttga
     6001 aagccgcatt cacagactgg tacatcctgt agacgggtat aagtatttct ggtacacctt
     6061 ttacttaagg cctgggtgat ttttctttta actttaacta agtcatcttt cacagagatc
     6121 agttcaatat ttgataggga ttccagcagc ttataacctc ttttttcttt aatattttca
     6181 atcggcttaa accggtctga tcctttctgc tggttgtgt
//
LOCUS       DATGCJ010000161         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_379644, whole genome shotgun sequence.
ACCESSION   DATGCJ010000161 DATGCJ010000000
VERSION     DATGCJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379644"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1123
                     /gene="fdhD"
                     /locus_tag="VKN64_07935"
     CDS             338..1123
                     /gene="fdhD"
                     /locus_tag="VKN64_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMA60208.1"
                     /translation="MMEDKHTQKYQITKVRKNEQKKVEDFIIKEQPLTIFVNGYELVT
                     LMTLPDKIKELTVGFLISEGILKDITEIKDLQLSHQNTIIRLKLDKEIEISLFKKRTL
                     TSGCGKGVTFSNLKDCGAQAIDKNNFSVLSQSINSLMTEMQKKAELFDRTGGTHTAAL
                     SDGNKLLYVIEDIGRHNTIDKIIGKAYLEDIDLKNKVILTSGRVSSEIIIKAVKQQIP
                     ILISRSAPTSAAVEIAREKDITLVGFTRGARYNIYNDLNRIKY"
     gene            complement(1179..1532)
                     /locus_tag="VKN64_07940"
     CDS             complement(1179..1532)
                     /locus_tag="VKN64_07940"
                     /inference="COORDINATES: protein motif:HMM:NF036294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HMA60209.1"
                     /translation="MPGGDGRGPAGAGPRTGRGLGYCNGYDSPGFTKPGPGMGRGFRR
                     GFGPGRGMRRGYYAGYARGGYAPNYAPNYNPQQPQQSEYNKEDEIDDLKEYAKDMKRE
                     LDNVVKRIEELENEE"
     gene            complement(1658..2023)
                     /locus_tag="VKN64_07945"
     CDS             complement(1658..2023)
                     /locus_tag="VKN64_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMA60210.1"
                     /translation="MKKIAVPTNGGNVAQHFGRCPEYTIFDVDGCDIKNSKVIDNPGH
                     EPGFLPRYLHDLEVDIVLAGGMGRKAFDLFNKNDIEVVTGARGKVEDSVKAYLNNELD
                     TESDICDHGKSHGSGHHHH"
     gene            complement(2035..2907)
                     /locus_tag="VKN64_07950"
     CDS             complement(2035..2907)
                     /locus_tag="VKN64_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA60211.1"
                     /translation="MKKITVISGKGGTGKTTVVANLAYLAQDLVLADCDVDAPNLHLL
                     LNPEVNNSSIFKSNKLAIKDQEKCTNCGVCYDVCRFNAVDPVNYEIDEYKCEGCGVCV
                     AMCPEDALSLKLLDTGEIYNSTTRFGPMVHANLKIGSDNSGKLVSEVREKAVETAEEQ
                     DKDLLLIDGSPGIGCPVISSITGVDLVLFVTEPSKSGFSDLKRVAQVIQHFNIPALAV
                     INKYDINEKISREIEEYLKEEDIELVGKISYSSDVVDSLRNAQLIVEQEQDNKVSKEI
                     EGIWTKIQNRISQN"
     gene            complement(2885..3751)
                     /locus_tag="VKN64_07955"
     CDS             complement(2885..3751)
                     /locus_tag="VKN64_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA60212.1"
                     /translation="MSSLKLTVLSGKGGTGKTTVSVNMALSLPNIQLLDADVEEPNSQ
                     YFLHPNFDNEEKIYRKVPEINQSKCTACRKCVDFCEYNALAMMLDEVLVFPEICHSCG
                     GCEKICPVDAITEVDRVLGVLKHANTDYDVDFWQGELNIGEESSVPVIEALKEKADRQ
                     KDVIIDAQPGTTCPTIEAVVDSDYGILVTEPTPFGLSDLKIASELMDELQIPHGVIIN
                     RAEEDSDYIIEDFCAEKGIDILLKIPFKREFAHLYSDGIPFVIEYPDWKDKFKSVFNE
                     IRGGLNEENYSN"
     gene            complement(3748..4107)
                     /locus_tag="VKN64_07960"
     CDS             complement(3748..4107)
                     /locus_tag="VKN64_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HMA60213.1"
                     /translation="MKIAVTAENDNGFESNVNPHFGRSPYFAIVDTETEEIELVKNEA
                     ARASGGAGVSAAQLISDRGVEALISGGVGPKAYTALARGNIEMYSIKDGTIRQAVEAF
                     EEGSLSQLTSPNGTPRK"
     gene            complement(4178..5437)
                     /locus_tag="VKN64_07965"
     CDS             complement(4178..5437)
                     /locus_tag="VKN64_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV damage repair protein UvrX"
                     /protein_id="HMA60214.1"
                     /translation="MKPVDYDNLPHQNILCVDMQSFFASIEAVSRGLDPVETPLAVLG
                     DKKRSGSVVLAASPALKKRYSIKTADRFYQIPDDPKIKLVEARMGLYVKISLEITELF
                     NNYVPLNAIHVYSIDESWIKLDGTERLFGDKWKVARMIKKDLLQKYGLNCSMALGPNM
                     FLAKVAMDNEGKKKGLVEWTYDDVEDKLWPLPLKKCWGIGGRLAKHFNKLGVKTVGEL
                     AHLPLEYLENKFGVMGTQLYYHAWGVDLSKVEGHYLDERKNLGRGITLYSDYQDIEKI
                     KTVIFELAEEVGKRARKRNLTGRTISLGLVYSREELEGGFHVQRTIDTHTNLTTVIFD
                     TAVVLLNENYRDQAVRKVRVSLGNFSNKANMQLNIFDNRAREIELMKVKDKLQDKYGY
                     QALYYGRSTKPGSIKERIKTTIGGHKA"
     gene            complement(5528..5755)
                     /locus_tag="VKN64_07970"
     CDS             complement(5528..5755)
                     /locus_tag="VKN64_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60215.1"
                     /translation="MLWLNNLAYTYIGNYPVIMYLGIITYLLLLVTAAIPRTNRKGWT
                     RIPIKYHIRLAYITGVLATIHGLMAISGYIG"
     gene            complement(5785..6342)
                     /locus_tag="VKN64_07975"
     CDS             complement(5785..6342)
                     /locus_tag="VKN64_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HMA60216.1"
                     /translation="MEKALSTIFILVLLLTGVSSILAAQEVNYQEVDGVFKPEEYDNT
                     LYVEALEMDIGWSVKEGNLVFGVRTVGLGWAAVGFNPTTMMRNANIIIGDVDEEGSLS
                     IEDHFGVSNTGHRADEERNILQAAGSESEEGTVIEFVIPLDSGDDMDRKLEAGQEYKV
                     ILAYHMSSNSFKIRHSNRTSVNIQL"
     gene            complement(6407..6736)
                     /locus_tag="VKN64_07980"
     CDS             complement(6407..6736)
                     /locus_tag="VKN64_07980"
                     /inference="COORDINATES: protein motif:HMM:NF020438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HMA60217.1"
                     /translation="MDINDRGNIKWTSLMLVEHRKKLEELKESEDEKKRPELDEQIYE
                     IFDYKIKEALQKNIKVKITYFADKEYKDIETYLKDYDTYQKKLILKYENPKEIFFQDI
                     IDISLLS"
     gene            complement(6808..>7836)
                     /locus_tag="VKN64_07985"
     CDS             complement(6808..>7836)
                     /locus_tag="VKN64_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60218.1"
                     /translation="KNRVIKKYKISKIKSNIWNNFDQIVTLKVKDNSKNYRIEGLQYK
                     LSNKNYRFLLKKQALNGQNKLFFKLTRIFKRNQINARISADNFGTDQINLTGELDYHF
                     KTGPKISLSGKVIYNPTEKKIDKYIKMGLLIPFTLKLPERKSKEVKGRVITRQGERAK
                     GVILSINGVKVKTDNKGKFNCYVKAKQDLLIKVLDLGEYSEQYVMSKDLPYFIENYNN
                     APLKLKLIKYGSLQIEIKKNEKSQNKYLKAIQSKEKGKVYVKLQNKYNYFIQKYKDHS
                     SLKFDRIPPGRYKIMIKNNSNKYDYTLKRKVIRIQSGDNSLKIRQVRKNNLNLKLKEA
                     TEELQIKN"
BASE COUNT         2303 a         1595 c         1169 g         2769 t
ORIGIN      
        1 agccttattc cagtttttaa gtaagaagtt caacttttgt tgtttgattt tagttggttt
       61 atagagtttt agatggtgta tggtaatata gtatttatca gacaacttaa tcacctgcat
      121 ttgattatag tattgatgta agtttataca agaactcatt tgtaagtaga ttgtgtactt
      181 ttgaattgta tgtcaactct atattaatta tatcttatat aattagtatt atcaagaaaa
      241 tatagaaaaa aatggaaagg aaggtgaaga acgtagcaat tcctctccac ctaaatcaaa
      301 ggcaagattt agatggagtt tccttgctac atttttaatg atggaagata aacacaccca
      361 aaaatatcag attacaaaag tgcgtaaaaa tgaacaaaaa aaggttgaag attttatcat
      421 aaaagaacag cccttaacta tttttgtaaa tggttatgaa ctggtcaccc tgatgacttt
      481 acctgataag ataaaagaat tgacggttgg atttttaatc tcggaaggta ttttaaaaga
      541 tataaccgaa ataaaagatc tgcagttatc ccatcaaaat acaattatta gactaaaact
      601 agataaagag atagaaataa gtctttttaa aaaaaggacc ttaacttcgg gctgtggaaa
      661 gggagttacc tttagtaatt taaaagattg cggagcccag gctatagata aaaataattt
      721 cagtgtactt tctcaatcaa taaactctct tatgaccgaa atgcaaaaaa aggcggaact
      781 attcgatagg acaggcggca ctcataccgc tgccctatct gatggaaata agttattata
      841 tgttattgaa gatattggac gccataatac aattgataaa ataattggaa aagcttattt
      901 agaagatatc gatttaaaga ataaagtaat actaaccagt ggacgcgtct cttcagaaat
      961 aattataaag gctgtaaaac agcaaattcc tattttaatt tcgcgctctg ccccgacctc
     1021 tgctgctgta gaaatagcaa gagaaaaaga tattactctt gttggcttta cgagaggtgc
     1081 tagatataat atttataatg accttaatag aattaaatac tgatatagaa aatcccgccc
     1141 tactatggac gggatttttt tattctatat attttttatt attcttcgtt ttcaagttct
     1201 tctattcttt ttactacatt gtctagctct ctcttcatat cttttgcata ttctttgaga
     1261 tcatctattt catcttcttt attgtattcg ctttgttgag gttgttgagg attatagttc
     1321 ggtgcatagt taggagcata gcctcctcta gcgtatcctg cataatatcc tcgtctcata
     1381 ccgcgtcctg ggccaaaacc tcttctaaag cctctgccca tacctgggcc tggttttgtg
     1441 aatccaggag aatcgtaacc attacaatat cctaatcctc tacctgttct tggtcctgca
     1501 ccagctggtc ctcttccatc tccgcctggc atatctatca ctcctttttt agaatttaat
     1561 ctttattatt tactgtactt atattataac gtagttatga acatatgtca ataactattt
     1621 aaaaaaacac ccggctaaaa gccggatata tgatttttta atgatgatgg tggcctgagc
     1681 cgtgggattt cccgtgatca cagatatcac tttctgtatc taattcatta tttaaatatg
     1741 ccttaacact atcttcaact tttcctcggg ctccggtaac tacctcaatg tcatttttat
     1801 tgaatagatc aaatgctttt ctacccattc cacccgctaa aactatatct acttcaagat
     1861 catgcaggta acgaggtaaa aatcctggtt catggcctgg attatcaatt acttttgaat
     1921 ttttaatgtc acaaccgtct acatcaaata tggtatattc gggacatcta ccaaaatgtt
     1981 gagcaacatt tccaccatta gtaggcacag ctattttttt cactaaatat cacctcaatt
     2041 ttgactgatt ctgttttgaa tctttgtcca aattccttct atttctttac ttaccttatt
     2101 atcttgctcc tgttctacaa tcaactgagc attcctaagg ctatcaacca catcggaaga
     2161 ataagagatc ttgccgacta gttcaatatc ttcttctttt agatactctt ctatctcccg
     2221 gctaattttt tcatttatat catatttatt gatcacagcc agagccggga tattgaaatg
     2281 ttgaataacc tgagccactc tttttaagtc tgaaaatcct gatttagatg gctctgtaac
     2341 aaaaagtacc aaatcaacac cggtaatgga agaaattacg ggacaaccaa ttccgggcga
     2401 cccgtcaatt aagagaagat ctttatcttg ttcttcggca gtctctactg ccttttctct
     2461 tacttcactg accaatttac ctgaattatc agagccaatt tttaaattag catgaaccat
     2521 aggtccaaat ctagtagtag aattatatat ttccccagta tctaaaagtt taagggaaag
     2581 tgcgtcctcc ggacacatcg ctacacagac cccacagcct tcacatttat attcatcaat
     2641 ctcataattt acggggtcta ctgcattaaa tctacataca tcatagcata ctccacaatt
     2701 cgtacatttc tcttgatctt ttatagctag tttattactt ttaaatatac tggaattatt
     2761 aacttcagga tttaataata agtggaggtt gggagcatcc acatcacagt ctgctaagac
     2821 tagatcttgg gctaaatagg ccagattagc tacaactgtg gttttgccag ttcccccttt
     2881 accactaatt actgtaattt tcttcattta gaccacctct tatttcattg aatactgatt
     2941 taaatttatc tttccaatct ggatattcta ttacaaatgg tattccatct gaatataggt
     3001 gggcaaattc tcttttaaag gggatcttaa gtaaaatatc aatccctttt tctgcacaga
     3061 aatcttcaat gatataatca ctgtcttctt ccgctctatt gattattacg ccatgcggaa
     3121 tttgcagttc atccatcagt tcactggcta ttttcaaatc acttaaacca aagggagtcg
     3181 gttctgtcac taaaattcca taatcactgt caactacagc ctcaatagta ggacaggtcg
     3241 tacccggttg agcatctatt attacatctt tttgtcggtc ggctttttct tttaaggcct
     3301 ctattacagg tacagaactt tcttcaccta tatttaattc tccctgccaa aaatctacat
     3361 cataatccgt gttagcatgt tttaagacac ctaataccct atcaacttca gtaatcgcat
     3421 cgactggaca aattttttca catccaccac aactatggca gatttcagga aagactaata
     3481 cttcatctaa catcatagca agggcattat actcacaaaa atcaacgcat ttacggcagg
     3541 ctgtacattt actctggttt atctcaggta ctttcctata tattttttct tcattatcaa
     3601 aattggggtg cagaaaatac tgagagttag gttcctcaac atcggcatct aaaagctgta
     3661 tgttgggtaa agacaacgcc atatttacag ataccgttgt tttgcctgta ccaccttttc
     3721 cgctgaggac agtcagcttc aaactgctca cttcctgggg gttccgttgg gagaggttag
     3781 ctgtgagagt gaaccctcct cgaaagcttc tacagcctga cggattgtac catctttgat
     3841 actatacatt tctatattgc ctcttgctaa agcagtatag gctttcggac caacaccacc
     3901 tgatattaaa gcttctacac cccggtcaga tatcaattga gctgcactta caccagcccc
     3961 gccggaagca cgggctgctt cattttttac caattcaatt tcttcagttt cagtatctac
     4021 aattgcaaaa tagggagaac gaccaaaatg ggggtttaca ttactttcaa atccattatc
     4081 attctcggct gttactgcta ttttcatatg atcaactcct tgtttattta tcactaatcg
     4141 gtaatattat accactgtaa tgaacatatg tcaattatta ggccttatgt cccccaatgg
     4201 tagttttaat cctttctttg atactgccgg gctttgtact gcgcccgtag taaagggcct
     4261 gataaccata tttatcctgc agtttatcct taactttcat cagctctatt tccctggctc
     4321 tattgtcaaa tatgttaagc tgcatatttg ctttattaga aaaattaccg aggcttaccc
     4381 ttactttgcg gacggcctga tccctataat tttcattaag caaaacaact gctgtatcga
     4441 aaataacggt ggtaaggtta gtatgagtat ctatagtcct ttgaacatga aatccgcctt
     4501 ctaactcctc tcggctgtag accagtccca gggaaattgt cctgccggtc aaattgcgtt
     4561 tgcgggccct tttacccact tcttcggcca actcaaagat aacagttttg attttttcta
     4621 tatcttgata atcactgtaa agggtaatcc ctctccccaa attttttctc tcatccaggt
     4681 aatgtccttc tactttagaa agatcaacac cccaggcatg ataataaagt tgggtgccca
     4741 ttacaccaaa tttattttcc aaatattcaa gcggcagatg ggcaagttca cctaccgttt
     4801 tcacacctaa cttgttaaaa tgttttgcta accgaccgcc aataccccag cacttcttta
     4861 aaggtaaggg ccagagcttg tcctctacat catcataagt ccattccact aaccccttct
     4921 ttttaccttc attatccatg gctaccttag ctaaaaacat attggggcct aaggccatac
     4981 tgcagtttaa accatacttc tgcaggagat cttttttaat catacgggct actttccatt
     5041 tatcaccgaa gagccgttcc gtaccatcca gtttaatcca ggactcgtca atagaataaa
     5101 catgaatagc atttaaaggt acataattgt taaaaagttc ggttatttca agagaaatct
     5161 ttacataaag acccatacgt gcttctacta attttatctt ggggtcatcg ggaatttgat
     5221 agaaacggtc ggcggtcttt atactgtatc tctttttaag ggcaggtgaa gcggccagca
     5281 caactgaacc actccgcttc ttatctccca aaactgccag aggagtttct accgggtcca
     5341 gaccgcgtga aactgcttct atacttgcaa aaaaggactg catgtcaaca cataagatat
     5401 tttggtgagg taaattgtca taatctacag gtttcaatct aaccacctcg aacatatgtt
     5461 ttatatttct gtttggtata taaatattat catacatatg ttcgtttttc aacaaaaaaa
     5521 taaccggcta accaatatag ccggaaattg ccatcaaacc gtgtatagtt gctaaaacac
     5581 cggttatata ggcaagtctg atatgatatt ttatagggat ccttgtccac cctttccggt
     5641 tggtcctcgg aattgctgct gttacaagca gtagtaagta agttataatc cccaggtaca
     5701 taataaccgg gtaatttcct atatatgtgt atgctaaatt attcaaccac agcatgatat
     5761 tccacctcag attattttat atttttataa ttgaatattg acagatgtcc tattactgtg
     5821 cctaatttta aaactgttac ttgacatgtg ataggcaaga atcactttat attcctggcc
     5881 cgcttctaat ttgcggtcca tatcatcacc actgtctaaa ggtattacaa actcgataac
     5941 tgttccctct tcactttctg agccggcggc ctggagtata ttcctttctt catctgcccg
     6001 atgtccggta ttggagactc caaaatgatc ttcaatactt aaactgcctt cttcatccac
     6061 atccccaatt atgatgttag catttctcat catagtagta ggattaaaac ctaccgcagc
     6121 ccaacccagg ccaactgttc taactccaaa aaccaagtta ccttctttca cggaccagcc
     6181 aatatccatt tccaatgctt ctacatataa tgtattatca tattcctctg gcttaaagac
     6241 tccatccact tcttgataat taacttcctg agctgccagg atagaagaaa cacccgtcaa
     6301 caataaaaca aggataaata ttgtactcaa ggctttttcc atcatttcgc cctccttctc
     6361 tactcctcgt aatataataa ttgaccaaaa agcagaatta tcctgcttaa cttaaaagac
     6421 ttatatcgat aatatcttga aaaaaaatct ccttagggtt ttcgtattta agaattaatt
     6481 tcttttgata ggtatcataa tcctttaagt atgtttctat atctttatat tctttatcgg
     6541 caaaatatgt gattttaact ttgatatttt tttgcagtgc ttcttttatc ttataatcaa
     6601 agatctcata gatctgttca tccagttctg gccttttctt ttcatcttcg ctttctttaa
     6661 gttcttctaa cttttttcga tgttctacta acataagaga agtccactta atatttcccc
     6721 tgtcattgat atccattaac atcacccctt ctaatgcgaa catatgttct aatattatta
     6781 tagcattaga agtgagcttt ggcaatctta atttttaatt tgtagttctt ctgtagcctc
     6841 ttttagtttt agattgaggt tgttttttct tacttgccgt attttaagac tgttatctcc
     6901 actctgaatt cttattacct ttctttttaa agtataatca tatttatttg aattattttt
     6961 tatcataatt ttatatctac ccgggggaat tctatcaaat ttaagggaag aatgatcttt
     7021 atatttttgg atgaaataat tatatttatt ctgaagttta acatatactt tacctttttc
     7081 cttagattga atggccttaa gatatttgtt ttggcttttc tcattctttt ttatctcaat
     7141 ttgtaaactt ccgtacttta ttaactttaa ttttaaaggt gcattgttat aattctcaat
     7201 aaaataagga agatctttac tcattacata ttgttcactg tattctccta aatctaatac
     7261 cttgattaat aaatcctgct tggctttaac ataacagtta aatttacctt tattgtccgt
     7321 ttttaccttc actccattta tgcttaagat aactcccttt gccctttcac cctgtctggt
     7381 aattactctg cctttcactt ccttactttt ccgctctggc agttttaaag taaaaggaat
     7441 taataacccc attttaatat atttatcaat ctttttctcc gttgggttgt aaattacttt
     7501 tccgcttaaa gatatttttg gaccggtttt aaaatgataa tctaattcac cggttagatt
     7561 aatctgatct gtaccaaagt tgtctgcact aattctagca ttaatttggt ttcttttaaa
     7621 aattctagtt aacttaaaaa ataatttatt ttgtccattt agggcttgtt tttttaataa
     7681 aaaacggtag tttttattac ttaacttgta ttgcagccct tcaatacgat aatttttact
     7741 attatctttt acttttaaag taacaatctg atcaaaattg ttccaaatat tactttttat
     7801 ttttgaaatt ttatactttt tgataactct attttt
//
LOCUS       DATGCJ010000162         6308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_335182, whole genome shotgun sequence.
ACCESSION   DATGCJ010000162 DATGCJ010000000
VERSION     DATGCJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335182"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VKN64_07990"
     CDS             <1..265
                     /locus_tag="VKN64_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HMA60219.1"
                     /translation="IEARIIGMFIMEDEKGRDEKILGVPVGDPRFDNIESIDDLESHI
                     LKEFQHFFEVYKDLEEKKVSIEGWADIKEAVAVIKKAKENYQK"
     gene            283..879
                     /locus_tag="VKN64_07995"
     CDS             283..879
                     /locus_tag="VKN64_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA60220.1"
                     /translation="MKVIGILGSPVKRGTNSLLQNALEALAAEGVETELLHITDYDLQ
                     FCQGCNTCLREGRCVLEDDLTKIGDKLIEADGIIMASPSYFGSPTAQLKNLMDRSRYL
                     KMQDHKLKDKLLGVISSSGLNQGGGQSTIEDINRFGLTHGMLIIGPAATPQTDANMVV
                     GTSETEEGWQRVKDDKKAVKLAKNLGSRMADTLDQLNK"
     gene            1063..1929
                     /locus_tag="VKN64_08000"
     CDS             1063..1929
                     /locus_tag="VKN64_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA60221.1"
                     /translation="MKIREIKAKKPLYKHKKKGLPYDYDLNIYRGCSHGCIYCYGRKS
                     HRYLGSSSFDTEIFVKTNIAEQLDEFLTNNDLKDSIINLGGVCDSYQELEQEYRLMPK
                     VLKVLIKHEQPIIITTKSDLIIRDLDLIEELSNKAEYVNIALTITNTKDEITAKLEPN
                     TSRPAARFKALKRLKDKAMYTALHFMPILPYFSDDKASLEQITRWAAEADVDYMLSGM
                     PYLTKNIKPRYYNFLKEKYPYYLREYQKLYNRGGADKKYKAKVHNYLAEMRKKYNVNN
                     SYVKFLPKNRNR"
     gene            1934..2716
                     /locus_tag="VKN64_08005"
     CDS             1934..2716
                     /locus_tag="VKN64_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMA60222.1"
                     /translation="MQKTKNNLINFFIITIFLSWFFWILSILINRNIISIGVNTNILH
                     ILGAFIPSLIGIYFIYRKNNKKSVIEFLKKGIDYKFDLKYYLYLFIFPLILLISYLIS
                     GLFLELNLEDSLLNEPKMIPIAFVYILILGGPLGEEYGWRGFALPRLNMIFKPLYSSI
                     ILGFIWSIWHLPLFFMEGSTQQGINYFGYTIFTILIAIIFTILFIRTGGSIFSAILFH
                     TMVNLGWGIFPVFYNLSISIVVLVLLSIVTIFLVYNNREIMV"
     gene            2746..3519
                     /locus_tag="VKN64_08010"
     CDS             2746..3519
                     /locus_tag="VKN64_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMA60223.1"
                     /translation="MVYSTRKIDNEFVTEKRIEVGEIPCLQFYPDNSREKLPTIIYYH
                     GWSSNKEFQRFKANVMASYGYQVIVPDSIHHGERNPIKHEEEGMLEKYLLKTIVTSIE
                     EAPVLINYVKEIDRTDKDRMGVMGTSMGGFISSGIFAQQQILKTLVVLNGACAWHKLE
                     AFDQQEHTDEYWNYRNELVKYNPADHLDLFNERPVLLLHGDSDTSVPIEGQRYFYQKA
                     VEHYDNKNRIELVEVPRMDHYISTGMWENAIAFNEDYLK"
     gene            3538..4269
                     /locus_tag="VKN64_08015"
     CDS             3538..4269
                     /locus_tag="VKN64_08015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HMA60224.1"
                     /translation="MPANGIATGIINLILFLIVVIARRLISKEGINTFLISRPRKRWK
                     LFWEGCLIGFILILVYVFLTVLLGVGELSFSLNLTSDFIFIFLSYLFGFAAVSLFEES
                     LFRDYILLKFKNKFSASEAVFISSLLFGLIHIISYITVGSIAILGLLNAFLIGVLLSI
                     IVIATHSILWPLGFHMVWNLTQTIFLIERSSGINLRIDKGILAGADMVPEAGIIVTLV
                     LIILAVYMLNRFDCAHIMPKSIGKG"
     gene            4397..4951
                     /locus_tag="VKN64_08020"
     CDS             4397..4951
                     /locus_tag="VKN64_08020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HMA60225.1"
                     /translation="MTEKDKMLNGEFYDPMDTELVTDRMEARKLLYSFNNSKPSDGIR
                     RKETIKQLFGKTGSRLFIEPPFQCDYGYNIEVGENFYANFDCVILDVNKVVMGDDVQL
                     GPGVHIYTAVHPTDPEERMSKTEYGEEIEIGNNVWIGGGAIICPRVKIGSNTTIGAGS
                     VVTKDIPENVLAVGNPCKVIKKFD"
     gene            4963..5766
                     /locus_tag="VKN64_08025"
     CDS             4963..5766
                     /locus_tag="VKN64_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HMA60226.1"
                     /translation="MSEKEVIELSDKVYTRKILGNEFKKIGLKPGMNVLVHSSLSKIG
                     WVTGGAVSVIQALMDVITKEGNIVMPAHSGDYSDPTNWSNPPVPEAWIDDIKENMPAF
                     RKAVTPTRGIGIIPEIFRSFPDVKRSYHPCFSFTAWGKDSDYITKDHSLDYGLGEDSP
                     LGKFYELAGYILLIGVNHDRNTSFHLSEIRSGVRGEFNNEGPILVNGERVWQKFKDIE
                     FDSDVFNKIGEAYEDQRDIKTGKIGLAPSKLFNQREAVDFAEKWLIENN"
     gene            5794..6300
                     /gene="def"
                     /locus_tag="VKN64_08030"
     CDS             5794..6300
                     /gene="def"
                     /locus_tag="VKN64_08030"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMA60227.1"
                     /translation="MAVKKIYQLGSEVLRKKSKEVENFDEELKDKVQDLKDTLLNFQK
                     EYGIGRAIAAPQIGYHQKIVYYNKGDEKLLMVNPEIIEKSEEQFSVWDSCFSFRVAFF
                     VKIKRAKKIKVKYQDINGKEYIEDFIWDLSELFQHEIDHLHGILATDHLEDNKNIIMR
                     EIWEEKYR"
BASE COUNT         2215 a          880 c         1282 g         1931 t
ORIGIN      
        1 aattgaagcc cgtattatcg gcatgtttat catggaagat gagaaaggta gagatgaaaa
       61 aatcctggga gtaccggtcg gtgatccgcg ttttgacaat atagaatcaa tagatgacct
      121 ggaatcacat atcttaaaag agttccagca tttctttgaa gtatacaaag acttagaaga
      181 gaagaaagtt agtatagagg gctgggcaga tattaaagaa gcggtagcag taattaaaaa
      241 agcaaaagag aattatcaaa aataaaacag aggtggtaat caatgaaagt gatcggtatt
      301 ttaggaagtc ctgttaaaag aggtactaat agtctcctgc aaaatgcttt agaagctctt
      361 gcagccgaag gtgtagaaac agaactacta catataacgg actatgattt acagttctgt
      421 cagggctgta atacctgtct aagagaaggc agatgtgtgc ttgaagatga tctgacaaaa
      481 attggtgata agttaattga agcagatgga ataattatgg cttctccctc ttattttggt
      541 tcacctactg cccaacttaa aaatttaatg gaccgcagcc gttatctcaa gatgcaggac
      601 cataagttga aagacaaact attaggagta atctcttctt ccggtcttaa tcagggcggg
      661 ggacagagta caattgaaga tattaaccgt ttcggcttaa cacacgggat gcttatcatc
      721 ggaccagctg ctacacccca gaccgatgct aacatggtgg tcggtacttc ggaaacagaa
      781 gaaggctggc agcgggtcaa ggatgataaa aaggcagtca aacttgccaa aaatcttggt
      841 agtagaatgg cagacacgtt agatcagtta aataaataac ccaaagattt aagtccctgc
      901 tgaagccagc agggatattt ttatatcttg ctaaaaatat gtaataatac tttaaattta
      961 ttaagaagta agatagaata tattataaag agttgtgaaa taattaactt atgtaaagat
     1021 taagttaaaa atagtaaaat gatattgata ggactgaaat taatgaaaat cagagaaata
     1081 aaggctaaaa agcccctata caaacataaa aagaagggac ttccctatga ttatgattta
     1141 aatatatatc ggggctgttc acacggctgt atctattgtt atggcagaaa aagccataga
     1201 tatttaggtt ctagtagttt tgatacagaa atatttgtaa aaacaaatat tgctgaacag
     1261 ttggatgaat ttttaaccaa taatgattta aaagatagta taattaattt aggaggagtt
     1321 tgtgacagtt atcaggaatt agaacaagaa tatcgattaa tgccgaaggt attgaaagta
     1381 ttaattaaac atgagcagcc tatcataatt accacaaaat ctgacctgat aataagggat
     1441 ttggatttaa ttgaagaact atctaataag gcagaatatg ttaatattgc tttaacaata
     1501 accaatacaa aagatgagat tacagctaag ttagaaccaa atacaagtag gccggccgcc
     1561 aggtttaaag ctcttaaaag attaaaggat aaggctatgt atactgcctt gcatttcatg
     1621 ccgattttgc cctatttttc agatgataaa gctagtttgg agcagataac tcgctgggca
     1681 gcagaagcag atgtggacta catgttaagc ggtatgccct atctaactaa aaatattaaa
     1741 ccgagatatt ataatttttt aaaagaaaaa tatccttatt atttaagaga ataccaaaag
     1801 ttatataatc gcggtggagc cgataaaaaa tataaagcga aagtacataa ttatttggct
     1861 gaaatgagga aaaaatataa tgtgaacaat agttatgtaa agtttctgcc taaaaatagg
     1921 aataggtgat taaatgcaaa aaactaaaaa caatttaatt aacttcttta ttattactat
     1981 ctttttatct tggttttttt ggatcttatc tattttaatc aatcgaaata taattagtat
     2041 tggagttaat acaaatattt tacatattct aggggctttt atcccttctc taataggtat
     2101 atattttatt tatagaaaaa acaataaaaa aagtgttata gaatttttaa aaaaaggtat
     2161 tgattataaa ttcgatttaa aatattatct atatctattt attttcccct taatattatt
     2221 aatatcttat ctcatttctg gtttgttttt agagttaaat ctggaagata gcttactcaa
     2281 tgaacctaag atgattccga ttgcttttgt atatatatta attttaggcg gtcctcttgg
     2341 agaagagtac ggctggcgcg gttttgcttt gccaagactg aatatgattt ttaagccgct
     2401 ttattcaagt attatattag gatttatctg gtcaatctgg catcttccgt tatttttcat
     2461 ggaaggtagt actcagcagg gtattaacta ttttggctat actatcttta ccatattgat
     2521 tgcaattatc tttacgatat tatttattag aacggggggc agtatttttt cagctatttt
     2581 gttccataca atggttaatc taggctgggg aattttccct gtattttata atctttctat
     2641 aagtatagtt gtacttgttt tattgtccat tgtaacaata tttttagtct ataataacag
     2701 ggaaatcatg gtctaaacta aattaatata ataggggtga taaaaatggt gtattctaca
     2761 agaaaaatag ataatgaatt tgtaacagaa aaaagaattg aagtaggcga aattccctgt
     2821 ttgcaatttt atcctgataa ttcaagggaa aagttaccta ctatcatata ttaccacggc
     2881 tggtcttcta ataaagagtt tcagagattt aaagcaaatg tcatggcttc ttatggatat
     2941 caagtaatag tacctgattc aatccatcat ggggagagaa atcccatcaa acatgaagag
     3001 gaaggcatgt tagaaaagta cttacttaag acaattgtaa cttctattga agaagctccg
     3061 gttttaatta attatgtaaa agaaatagat agaacagata aagatagaat gggagtcatg
     3121 ggtacatcta tgggcggctt tatttcatcc ggcatatttg cacagcagca gatacttaag
     3181 accttagtag tactaaatgg agcctgtgcc tggcataaac tggaagcctt tgatcaacag
     3241 gagcatactg atgaatactg gaactatcgg aatgaattgg ttaagtataa cccggcggac
     3301 catctagacc tttttaatga aagaccggtt cttttactac acggagacag tgatacttca
     3361 gttccaattg aaggtcaaag gtatttttat caaaaggcag tagaacatta tgacaataaa
     3421 aacaggatcg agttagtaga agtccctagg atggaccact atataagtac cggcatgtgg
     3481 gaaaatgcaa ttgcttttaa tgaagattat ttaaaatgat aaggagagga ttcttttatg
     3541 ccagctaatg gtattgctac cggtataatt aatttaatat tattcctaat agttgtaata
     3601 gctaggcgtt taatctcaaa agaaggaata aatacatttt taataagtcg accgcgaaaa
     3661 agatggaagc tattttggga aggctgtcta attggattta tcctgatttt agtatacgtt
     3721 tttttaacag tattactcgg agtaggagag ttaagtttta gtttaaacct tacttcagat
     3781 tttatattta tatttttatc ctatttattc ggttttgccg ctgtttcctt atttgaagaa
     3841 tcactattta gagactatat attactcaaa tttaaaaata aattttctgc ctcagaagca
     3901 gtttttatat cttccttact gtttggatta attcatataa taagctatat cacggtcggt
     3961 tcgattgcaa ttttagggct attaaacgct tttttaattg gagtactgct atcgataatc
     4021 gtgatagcaa ctcattccat actctggcct ctaggctttc atatggtttg gaatttaacc
     4081 caaacaatct ttttaatcga aaggtctagc gggattaatt taaggataga taagggtatc
     4141 ttggcgggag ctgatatggt cccagaagcc ggaataattg tgacacttgt tttgatcata
     4201 ttagcggtct atatgcttaa tagattcgac tgtgcccata ttatgccgaa gtcaatagga
     4261 aagggctgag aggcttagat gataataacc aggtatggtt atcaaattaa tttttatttt
     4321 taaaaagtag attttttaag gaataattaa gatgaaaacc ggggctattt gcttaaaatt
     4381 aaagggggta ttaagattga ccgaaaaaga taagatgtta aacggtgagt tctatgatcc
     4441 catggatact gagctggtaa cggatagaat ggaagcccgt aaactattat atagctttaa
     4501 taattcaaaa ccttcggacg gtatccgtag aaaagagacc ataaaacaac tttttggaaa
     4561 aacaggctca agattattta ttgaaccccc gttccaatgt gattacggat ataatattga
     4621 agtaggagaa aacttctatg ctaactttga ctgtgtaata cttgacgtaa ataaagtggt
     4681 catgggtgat gatgtccagt taggaccggg tgtacatata tacacagcag tccatcctac
     4741 tgatcctgaa gaaagaatgt caaaaaccga atatggtgaa gagatagaaa ttggtaataa
     4801 tgtctggatt gggggaggcg ccatcatctg tccgagagtt aaaatcggtt cgaatactac
     4861 tattggagct ggaagtgtag taacaaaaga tattccggaa aatgttctgg cggtaggtaa
     4921 tccctgtaaa gttattaaaa aatttgatta ggagcataaa gcatgagtga aaaagaagtt
     4981 atagaacttt ctgataaggt atatacaaga aaaatattag gaaatgagtt taaaaagatt
     5041 ggtttaaaac cggggatgaa tgtattggtt cattcttccc ttagtaagat aggttgggtg
     5101 accgggggag cggtttcggt catacaggct ttaatggatg tgataaccaa ggagggcaat
     5161 attgtaatgc cggcccattc cggagattac tcagatccca cgaattggtc taatcctccg
     5221 gtaccagagg catggataga tgatattaag gaaaatatgc ccgcttttag aaaagcagta
     5281 actccgacca gagggatcgg tattatacct gaaatcttta ggagttttcc ggatgtaaag
     5341 aggagttacc acccctgttt ctcttttact gcctggggta aagattcaga ttatataacc
     5401 aaagaccata gcctggacta tggtttggga gaagattccc ctttaggcaa gttttacgaa
     5461 ctggctggat atatattatt gataggagta aaccacgata gaaatacctc ctttcattta
     5521 tctgaaataa ggtcaggtgt gagaggcgaa tttaataatg aaggcccaat ccttgttaac
     5581 ggtgaaaggg tctggcagaa attcaaagat attgagtttg attcagacgt attcaataaa
     5641 attggagaag cttatgaaga ccaaagggat attaaaacag gaaaaattgg gttggcacca
     5701 tctaagttat ttaaccagcg ggaggcagtt gattttgcag aaaaatggct aatagaaaat
     5761 aattaagtaa tactatagag gaggtagcag gagatggcgg ttaaaaaaat atatcaatta
     5821 ggtagtgaag tacttagaaa aaaatctaaa gaggtagaga actttgatga agaactaaaa
     5881 gacaaagtac aggatttaaa agatacctta ttgaactttc aaaaagaata tggaataggg
     5941 cgggcgatag cagctccgca gattggttac catcaaaaaa tcgtgtatta taataaaggt
     6001 gatgaaaaat tacttatggt taaccccgaa atcatcgaaa aaagcgaaga acagttttca
     6061 gtctgggaca gctgttttag ttttagagtg gctttttttg ttaaaattaa gagagccaaa
     6121 aagataaaag tcaagtatca ggatataaac ggcaaagaat atattgaaga tttcatttgg
     6181 gatctatcag aacttttcca gcatgaaata gaccacttgc acggtatttt agctacagac
     6241 catctggaag ataataaaaa tatcataatg agggaaatct gggaagaaaa gtatagatag
     6301 ggaggttc
//
LOCUS       DATGCJ010000163         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_335256, whole genome shotgun sequence.
ACCESSION   DATGCJ010000163 DATGCJ010000000
VERSION     DATGCJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335256"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1570
                     /locus_tag="VKN64_08035"
                     /pseudo
     CDS             306..1570
                     /locus_tag="VKN64_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1570..2370
                     /locus_tag="VKN64_08040"
     CDS             1570..2370
                     /locus_tag="VKN64_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA60228.1"
                     /translation="MFKVFYSLSKRPFVKEIAPNDLFMSDNFKEVKARLDYLKNTRGI
                     GVVVGEPGCGKTTALRFFAHSLNPSLFKIIYFPLSTGTVMDFYKGLASGLGEKPKTRK
                     VDLFQQIQQAVTNFYQEQKITPVFILDEMQLAANRFLSDLSILFNFSMDSQNPFLLIM
                     SGLPYFMDKLSLNQNQSLTQRIVMRYYLESLSKEEIAEYINHQLKLAGANHPIFTPQA
                     IEAISLRSRGLPRLVNNLALNSLLMGYQLKADNINEEIVSKACEEAGL"
     gene            2367..2600
                     /locus_tag="VKN64_08045"
     CDS             2367..2600
                     /locus_tag="VKN64_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60229.1"
                     /translation="MTDQKAVLRYYFDTGWMVVNNDLFIKLIHENIRDFDILLDDDYQ
                     TSRYFFNSYGELCVIIENQEFALSKTTGYDIEF"
BASE COUNT          872 a          578 c          516 g          932 t
ORIGIN      
        1 tcatctactt tagagaaact gcgaatggtc atccttgctt tgataacaaa aaagccataa
       61 aacttcttga aatgatcaaa gaatgcaagg tccccacctt cgcaaagagc ttccacaatc
      121 acttcaataa atgttttatg gtactttaat tttaccatag aagtgatctt ttgggaagta
      181 ttttttaaaa cacattttct ttcactagat aagggcttaa tcttaaaagt ataatgttat
      241 tatcaaaaat ataccctttt tagcctaaat atttaaggct aaactcccaa aggaggtgca
      301 atttaatgac tgataatgac agagagaaga tagccctgtt tcgttttgcc ctcatatcac
      361 ctatcctgag cgggcaggtt caaaaccaaa aggaatatct atcagaaatt acagccaaaa
      421 cccacaatgt cccctattat ggtcctaaag aatatgtacc caagaccatc caatgctggc
      481 ttagggatta tcgccggggc ggatttgatg catttgatgc actaaagcct aaaagacgtt
      541 ctgattacgg ccgcagccgt aaggtctatc ccaaacttaa ggaaaaactg gttactttaa
      601 gaaaagaaaa gcctgatatt tcggtttctc ttttctacga ccaactgctt actcataaag
      661 tgattaaacc ttctgatgta tcctactcca ccatctatcg gatattcaaa agagaaggtc
      721 tgctgactaa tactcctcgt aaagaagcac ataggcttcg ctttgcccat cctgatgtca
      781 atgccatgtg gcagggcgat tttatggtcg gcccttacct taagatcggt aaaaagaagg
      841 ttaaaaccca tctttttgct tttatcgatg actgctcgag aataattcct tatgctatgt
      901 ttttacccac cgaaagattc tcttctatta gcaaagtatt ctcagaagca attctaagaa
      961 gaggcttacc gcagctgctc tatgtcgata acgggaaaat ttaccggtcg ggtatgcttc
     1021 agtttgcctg cgcatcactc ggtattactt tggtccacac taagccctat gactcagctg
     1081 ccaaagggaa ggtggaaagg ttcttcctta ccgtcaggaa gaggtttatc cctcttctta
     1141 ttgaaaaaga actttcttca tttgagtatt taaacgagcg tttttcaact ggctggaaaa
     1201 agattaccat cgtaagatcc attctgccct tgggattacc cccatggaaa agtacatgtc
     1261 acaaatcgat aaggttaaaa tggtcgatga ccccaaaacc cttaaaaaca tcttctttaa
     1321 gcgggttaaa cgaaaggtaa aacacgatag aactatttct cttaacaaca ttttctatga
     1381 agtacccccc accttgatcg gcaaaaggat tgaggttcgt tttgaccctg aaacctttga
     1441 ccaggttctc atctatcaag aaggaaagat gattggccga gctaaaaagg ttatctttgc
     1501 tgataatgcc cggattaaaa gaaaaggcaa tatctctttt aaaaatatca taaaagaagg
     1561 tgacaactga tgtttaaagt cttttattcg ctttctaagc gtccttttgt taaagaaatt
     1621 gcacccaacg atctttttat gtccgataat tttaaggaag ttaaggcccg tcttgattac
     1681 cttaaaaaca cacgtggtat cggggtggta gtcggtgagc ccggatgtgg taaaaccacc
     1741 gccttaagat tttttgctca ttctcttaac ccatcacttt ttaaaattat ctatttccca
     1801 ctttctaccg gtacggtcat ggatttctac aagggtttag cctccggttt aggtgaaaag
     1861 cctaaaaccc gcaaggttga tctctttcag cagatccaac aggctgttac aaatttctat
     1921 caggagcaga agattacacc ggtctttatc ctagatgaga tgcagctggc tgccaaccgc
     1981 tttttaagtg acctcagtat cttatttaat ttttctatgg attcacaaaa tccttttctt
     2041 ttaattatgt caggacttcc ttatttcatg gataagcttt ctttaaatca aaaccagtcc
     2101 cttacccaaa gaatcgtaat gaggtattat cttgaatctc taagcaagga agaaatcgcc
     2161 gagtatatca accaccagct caagctagca ggagcaaatc atcctatctt tactccacag
     2221 gctatcgaag ctatttcact ccgttcccgc ggattacctc gcttggttaa caatctggcc
     2281 ttaaattcac tccttatggg ttatcagctt aaggctgata acatcaatga agaaatcgtc
     2341 tctaaggcct gtgaggaggc cggcttatga cagatcaaaa agcggtttta cgttattact
     2401 ttgacactgg ttggatggtt gttaacaatg atctctttat taaattaatc catgaaaaca
     2461 tccgggattt tgatatattg ttagatgatg attatcaaac ctcccgatat tttttcaata
     2521 gctatggtga gctttgtgtt attatcgaaa accaggagtt tgctttaagc aaaaccacag
     2581 gttatgatat tgagttctaa ttcgggggcg tctctaattt tagagatgcc tctttgctaa
     2641 tttattttga caaatatttc agattttcta ttttattttg attaattttc acattacagg
     2701 gtaatatttg tcctaattta aagtgatgaa ttttggaatt ttaatttgaa aattcacatt
     2761 atatgtttta tgttttaaga ttcttccctt ttaaaaggga agaaattttt tgaacttgta
     2821 ccttctatat tttattaagt tggacatact tggttttcaa aataataatt cgctgtttta
     2881 aagttgaaca tcttcctg
//
LOCUS       DATGCJ010000164         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_155807, whole genome shotgun sequence.
ACCESSION   DATGCJ010000164 DATGCJ010000000
VERSION     DATGCJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155807"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..743)
                     /locus_tag="VKN64_08050"
     CDS             complement(6..743)
                     /locus_tag="VKN64_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HMA60230.1"
                     /translation="MKKGNKYGLHRVIAPKGVLPQPAERIDNTMEIYDNEILIDVEIL
                     NIDSASFTQIKDEVGEDEEKIKEKMNEIVNKRGKHHNPVTGSGGMLIGKVAEIGEKLK
                     DRDLKVGDRIATLVSLSLTPLKIEKIKKVHKDIDQVEVEAQAVLFESGIYAKLPADMK
                     DTLALAVLDVAGAAAQTAKLVRPGDNVLIIGAGGKSGLLCLHEAKKRAGVTGTVMGMG
                     HSDSSCESIKELGAADISIQGDATEPV"
     gene            complement(765..1481)
                     /locus_tag="VKN64_08055"
     CDS             complement(765..1481)
                     /locus_tag="VKN64_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HMA60231.1"
                     /translation="MEIIKYNFEDLSALFINCTLKKSPRQSHTRGLIDVSKHIMEKHN
                     VDVEVIRAVDYDIATGVYPDMTDKNWDKDDWPAIQKKVLASDILVLGTPIWLGSKSSV
                     CSRVIERLYSYSGQVNDEGQYIYYGRVGGTIITGNEDGVKHCAMNILYSLQHLGYVIP
                     PQSDAGWIGEIGPGPSYLDEGSGGPENEFTNRNTTFMTWNLMHMAKMLKDNDGIPAYG
                     NQRTKWDEGDRFDNPNPEYR"
     gene            complement(1488..2303)
                     /locus_tag="VKN64_08060"
     CDS             complement(1488..2303)
                     /locus_tag="VKN64_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HMA60232.1"
                     /translation="MNKLIITAAICGAEVTKEDNPNLPITSEELAEEALKAEQAGASI
                     IHLHVRDEDGNPTQSKEVFKKAIDKMEEKGVKAIIQPSTGGAAGMTWQERIQPVYLKP
                     EMATLDCGTTNFGDDIFVNDLPLMRSFADEMDKYDVLPELECFEVGHIYNGLRLKKEG
                     LLKKHLHFDLVLGVPGAMPASVKNLLFMVEHLPEEATWTVAGVGRYQLELAYHTIIMG
                     GHVRVGFEDNIYYKKGELAESNAQLVKRVVRLAKEYGREIASPQEARKILEIN"
     gene            complement(2309..>2702)
                     /locus_tag="VKN64_08065"
     CDS             complement(2309..>2702)
                     /locus_tag="VKN64_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HMA60233.1"
                     /translation="ERLETEVMIRMRMSTNDAHYAGNLVDGAKMLELFGDVATELLIR
                     SDGDEGLFLSYDDVEFTAPVYAGDFIEAKGKIVKKGNTSRKMEFEAHKVIAPVEEAEF
                     DSACEVLDDPVLVCKASGTCVVPKERQR"
BASE COUNT          715 a          663 c          425 g          899 t
ORIGIN      
        1 agtgtctaca caggttcggt cgcatcaccc tggatactga tgtcagctgc gcctagttct
       61 ttaattgatt cacagctaga gtcactgtgg cccattccca tcacagttcc ggttactccc
      121 gctctttttt tagcttcatg caaacagagt aagccggact ttccgccggc tccaataatt
      181 aaaacattat cacccggtct tactaattta gcggtctgag cagctgctcc agctacatct
      241 aataccgcta aagccagagt gtctttcata tcagccggta gtttagcata gataccactt
      301 tcaaaaagaa ccgcctgggc ctctacttca acctggtcga tatctttatg caccttttta
      361 attttttcta ttttcagggg agttaaagaa agtgaaacta aagtagcgat tctatctccc
      421 actttaagat ctctatcctt taacttttct ccaatctcag ctactttacc aattaacatc
      481 ccgccggaac cagtcacagg attatgatgc ttacctcttt tattcactat ctcattcatc
      541 ttttccttta ttttttcttc atcttctccg acttcatctt taatctgggt gaaactggct
      601 gaatctatat ttaatatctc tacatcgatt aaaatttcat tatcatatat ttccattgtg
      661 ttatctattc tttcagccgg ttggggcaaa actccttttg gtgctattac cctatgtagt
      721 ccgtatttat ttcctttttt cattttttta ttacctccta gtaatcatct atattcggga
      781 ttgggattat caaacctatc tccttcatcc cattttgttc tctggttacc ataagctggt
      841 ataccatcat tatcttttag catcttggcc atatgcatca aattccaggt catgaaggtt
      901 gtattacggt tggtaaattc attttcaggg ccgccggaac cctcatctaa ataggacggt
      961 ccgggaccga tctcaccaat ccagcccgca tcggactggg gtggaattac atagcctaaa
     1021 tgctggagtg agtaaaggat gttcatcgcg cagtgtttta caccatcctc attacccgtg
     1081 attatagtgc caccaactct tccataatat atgtattggc cctcatcatt aacttggccc
     1141 gaataactat ataacctttc aattactcta ctacagaccg aagattttga accgagccag
     1201 atcggggtgc ccagtactaa aatatcagaa gctaaaactt tcttctggat ggccggccaa
     1261 tcatccttat cccagttttt gtcggtcata tcaggataaa caccggttgc aatatcataa
     1321 tcaactgccc tgattacctc aacatctaca ttatgttttt ccatgatatg tttagaaaca
     1381 tctattaaac ctctcgtatg tgattgacgg ggtgatttct ttaaagtaca gttgataaaa
     1441 agtgcactta gatcttcaaa attatactta ataatctcca cctccgatta atttatctct
     1501 aaaatcttcc ttgcttcttg aggactggct atttcccgac catattcctt agctaaacgc
     1561 acaactcttt tcactaattg agcattactt tcagccaact cacctttctt gtaataaata
     1621 ttgtcttcaa aaccaactct tacatgccca cccatgataa ttgtatggta ggctaattct
     1681 aactgatatc ggccgactcc agccacggtc caggtcgctt cttctggtaa gtgctcaacc
     1741 ataaatagca agttcttaac agaagcaggc atggctccgg gtacacctaa gaccagatcg
     1801 aaatgtaggt gtttttttaa taggccttct ttttttaacc tgagaccatt atagatatgc
     1861 cccacttcaa aacattctaa ttccggtaaa acatcatatt tatccatctc atccgcaaaa
     1921 cttctcatca agggtagatc attcacaaat atatcgtcac caaagttagt ggtaccgcag
     1981 tctaacgttg ccatctcagg ctttagataa actggttgaa ttctctcctg ccaggtcata
     2041 ccggccgctc ctccggtcga gggttgaatg atagccttaa ctcctttttc ttccatctta
     2101 tcaatcgcct ttttaaagac ttctttagat tgagtggggt tgccatcttc atctctcacg
     2161 tggagatgaa taatcgatgc tcccgcttgt tctgctttta aggcttcttc ggctaattcc
     2221 tctgacgtaa tcggtaaatt ggggttatct tctttggtaa cctcggcccc acaaatagcg
     2281 gcggtaataa ttaatttatt catggttatt atctctgcct ttcttttggt accacacagg
     2341 taccacttgc tttacagacc aatactggat catctaaaac ttcacaggca gaatcaaatt
     2401 ctgcctcctc aactggtgct attactttat gagcctcaaa ctccatttta cgggaagtat
     2461 tacctttctt aactatttta cctttagcct caataaaatc accggcataa acaggtgccg
     2521 taaattcaac atcatcataa gataaaaaga gcccttcatc accgtcactt ctaattaata
     2581 actcagttgc aacatcacca aagagttcta acatcttggc accatcgact aaattgccgg
     2641 cataatgggc atcatttgta ctcattctca ttcttatcat tacttctgtt tccaatcttt
     2701 cc
//
LOCUS       DATGCJ010000165         8948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_559989, whole genome shotgun sequence.
ACCESSION   DATGCJ010000165 DATGCJ010000000
VERSION     DATGCJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559989"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VKN64_08070"
     CDS             <1..954
                     /locus_tag="VKN64_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HMA60234.1"
                     /translation="ADLEFEEVCKLMSDIGYDGLEIACWGDHMDVKKAAEDMKYVKSK
                     KELLKKYDLECHALGAHLAGQCVGALYDPRLDNFVPDKVKGEPEAIREWAIQEMKYTA
                     QAAKNMGVKVVNGFTGSPIWKYFYSFPQTTEEMVEDAFDEIVELWSPIFDVFDENGVR
                     FALEVHPTEIAYDYYTTQRLFKAFDNRKTLGINFDPSHLIWQGVTPHILIRDFPEKIY
                     HVHMKDAAVNLDGKAGILGSHLTFGDTRRGWNFRSLGHGDVNFEDIIRELNNIGYTGP
                     LSVEWEDSGMDRIYGATEALDFVRNVDFKPNEAAFDDAMKG"
     gene            992..2497
                     /locus_tag="VKN64_08075"
     CDS             992..2497
                     /locus_tag="VKN64_08075"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HMA60235.1"
                     /translation="MIKLNEVLVKTKNIAKEFSGVRVLDDINIEIKKGEIFGIIGENG
                     AGKSTFIKILNGIYEQTEGQVFFEGKEVDMDVQKAKKIGINTIPQEFNLINDLNVYEN
                     IFLGKEYQKNKFLLDKDKMREETNRFLKRLDTEIDPNEKISNLSVAEKQMVEIAKAIA
                     YNSKLLIMDEPTTVLTPFEVKTLFKLMKDLVNEGITIIYISHNLKEVKEICDRVMVLR
                     DGEFICLEDADDLDEKEMANRMVGRELNQMFPPKVEAQGEKVLEVKNLNVKGLLEDIN
                     FDLKKGEVLGFSGLVGAGRTELAETIIGIREKTSGEIYINGKKVNIDSPSQAVKNDIA
                     YLPEDRQGVGILTNFTIPSNITLISLKKYTKLLINHKKVKEKAKYYVDEFNIQAPSID
                     TELEYLSGGNQQKVSLSKSLDTEPNIFIFDEPTRGIDVNAKMEIFNFIHELVQSGISV
                     IFISSELEEIIGMCTRVVVMRSGTITGILEGEHINEEEIMYHATGVKRGAI"
     gene            2500..3480
                     /locus_tag="VKN64_08080"
     CDS             2500..3480
                     /locus_tag="VKN64_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60236.1"
                     /translation="MKDKKKKEGKNFWDLIKNINFEKYGPLIALLVLFIISSFASPYF
                     LKFRNLTNILRQVSYTGIIALGMTFVIISAGIDLSVGSMTALVGGIVIMTLNYFGGGI
                     FAIFMAILVGILFGLVLGAVNGLIITRWNVAPFIVTLGTMSIFRSLTLYISDASEFRS
                     TSTIFPELGMGYFLKLPIPVWIFFGLTVLYSIVLNHTRYGRYICAVGSNQKVAKYSAI
                     KVKLTKFFAYVIVGINVGITAVLLSSRLNSISSSGAGTGYELDAIAAVIIGGTAMSGG
                     SGSVVGTLIGAIILGIVNNMLNMLGVSPYLQGTVKGLVIIGAVLIQRKKE"
     gene            3555..4526
                     /locus_tag="VKN64_08085"
     CDS             3555..4526
                     /locus_tag="VKN64_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60237.1"
                     /translation="MRKIALILTLVLVFSLTLSAVTFAQDEIRIGVTIPSADHGWTGG
                     VVYWAQQAIKDWEAKNDNITFFLETADSPATQVSVVEDLMIKEIDALVILAHDSAPLT
                     PVVKQASEEGIYVVSVDRGLTEPVADIYVAGDNPGLGRVSGEWMAKELNGEGDIVVLE
                     GIPCVINTQRVEAFEKVMEQYPGITIMDSQPANWSTQKGLEIMENYLQKYDKIDAVWA
                     QDDDVLKGVLQAYEESGRDDIKFFLGGAGSKDIVKMVMEGSDLVRADVTYPPSMIATG
                     ISMAVKAMQGETLDGFYQNELPSEVILRAELITEENAENYYFPDSVF"
     gene            4667..5998
                     /gene="xylA"
                     /locus_tag="VKN64_08090"
     CDS             4667..5998
                     /gene="xylA"
                     /locus_tag="VKN64_08090"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HMA60238.1"
                     /translation="MGEIFPDLPKIKYEGKESGNLLSFKYYNPEQKIRGKKMRDILRF
                     AVAYWHTFCHDGSDPFGDGTLQKPWVNIQDPLQRAEKKAAGAFEFMDKLGVDYFCFHD
                     RDIAPAGRDLKETNYNLDKIVEKIKGLMEETNINLLWGTANLFSHPRYANGAATSCDA
                     DIFAYASAQVKKAMEVTNYLGGKNYVFWGGREGYETLLNTDMKLEQDNMARFFSMAVE
                     HAEKIGFKGQFLIEPKPKEPTKHQYDFDVANVIAFLRKYDLEQYFKVNAEANHATLAG
                     HNFQHELRLARINNLLGSVDINQGDYLLGWDTDQFPSNIYMSTLAMYEILKNDGISPG
                     GLNFDARVRRPSKDPVDLLYGHILGMDSYAKGLIIADKMLRDRVFEDFIDNRYVSYNR
                     DIGLKIKNGQESFASLEEYVLEKEGEFKTSSGRQEMLEAILNKYILETNLT"
     gene            6016..7512
                     /gene="xylB"
                     /locus_tag="VKN64_08095"
     CDS             6016..7512
                     /gene="xylB"
                     /locus_tag="VKN64_08095"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HMA60239.1"
                     /translation="MSYLMGIDIGTSNVKIIVISAEGELVAIETSSYPLYSPQPGWSE
                     QNPTDWWNSTKEALKAIIEKNDINSDEIKGISLSGQMHSLVMLDENKEVIRPAILWND
                     TRTTRQCEEIYEQVGGLAKLIELVSNPALEGFTAPKLLWIKEHEPANYKKIRYIMLPK
                     DYIRYKLTDEIKSEVSDNAGTLLFDVEHKKWSDEILELLNISSDILPEVLYSTDIAGY
                     TTKELESLIGIKAGIPLIAGGADNACGAVGSGIIKDGRVMVSIGTSGVVLAQSDKPVP
                     DQKGRIHLFNHSYPDKFYMMGVMLSAAGSFNWLKEKMYDDKYSIKKLNELARNSKPGS
                     NGLVFLPYLNGERTPHADSNARGVYFGLSNKHDIRHIVRSTMEGVAFGLKDSFGLIKS
                     KDIKVEQIRVIGGGAKSSLWCQILSDIFEQEISLINIEEGPAFGAALIAGVGANVFAS
                     FESAEEKFIKIKKTIKPNSDNFKVYRHNYERYKSLYNSLKEDFALHNK"
     gene            7630..8151
                     /locus_tag="VKN64_08100"
     CDS             7630..8151
                     /locus_tag="VKN64_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60240.1"
                     /translation="MFKKVTIIALIFLISFSSIVFAQEWDTFSEKDDMSGVMIWYAGS
                     PKTTSNNPMSTPYYNGTKASLHVGTDGFSEWCYISFNKEPIIKNTEIKDCYNAFTTRL
                     EWDDDICHMDFTKEWGTSFIHFVEDKKAINKIMENNKVLLELDWYGEGKVHFEFTLNS
                     SADAIKKIREKTN"
BASE COUNT         3116 a         1240 c         1884 g         2708 t
ORIGIN      
        1 gcagatttag aatttgaaga agtctgtaaa ctgatgagtg atattggtta tgatggtttg
       61 gaaattgcct gctgggggga ccatatggat gtcaaaaaag cagctgaaga tatgaaatat
      121 gtaaaaagca aaaaggaact gctcaaaaaa tatgaccttg aatgtcatgc cctgggggcc
      181 catttggccg gacagtgtgt aggtgcatta tatgatcctc gcttagataa cttcgtacca
      241 gataaagtaa aaggtgaacc tgaagcgatc agggagtggg cgattcagga gatgaaatat
      301 accgcccagg ctgctaaaaa catgggagta aaagtagtaa atggttttac tggatctccg
      361 atttggaagt atttctattc tttccctcag actacagagg aaatggtaga agatgctttt
      421 gatgaaattg ttgaactatg gtctccaata tttgatgtat ttgatgaaaa tggggttagg
      481 tttgcccttg aggtccatcc gacagaaatt gcttatgatt actatacaac tcaaagatta
      541 tttaaagctt ttgataatag aaaaaccctg ggaattaatt ttgatcccag tcatttaatc
      601 tggcagggtg taactcccca tatattaata agagattttc ccgaaaaaat atatcacgtt
      661 catatgaaag atgctgctgt aaatttagat ggtaaagccg gaattttagg ttcacatctt
      721 acctttggcg atacaagaag gggctggaac tttcggtcac tcggacatgg agacgttaat
      781 tttgaagata tcatcaggga gttaaataat attgggtata ccggaccgct ttctgtagaa
      841 tgggaagact ccggtatgga tagaatatat ggggctactg aagccctgga ttttgtaaga
      901 aatgttgact tcaaacctaa tgaagcggcc tttgatgatg ctatgaaagg ataattttaa
      961 taagattatt aataattatg gcaggaagta ggtgattaaa ttgaatgaag tattggtcaa
     1021 aacaaaaaat atagctaaag aattttcggg cgtgagagtg cttgatgata ttaatatcga
     1081 aataaagaaa ggagaaatct tcggtattat aggagaaaat ggggccggca agtccacctt
     1141 tatcaagata ttaaatggta tttatgaaca gaccgaaggc caagtattct ttgaaggtaa
     1201 agaggtggac atggatgttc agaaggcgaa aaagattggt attaacacca ttccccagga
     1261 atttaactta attaatgacc taaatgttta tgaaaatata tttttaggaa aagagtatca
     1321 aaaaaataaa tttcttctag acaaggataa aatgagagaa gaaactaatc ggtttttaaa
     1381 gagattggat acagaaattg accctaatga gaagatcagt aatttaagtg tggcagaaaa
     1441 acagatggtg gaaatagcca aagctattgc ctataattcc aagttactga tcatggatga
     1501 accgaccact gtgcttacac cctttgaggt taaaaccctc tttaagttaa tgaaggatct
     1561 ggtaaatgaa ggtatcacta ttatctatat ttctcacaac ttaaaagaag ttaaagaaat
     1621 ttgtgatagg gtaatggtgc tgcgggatgg tgaatttatc tgtttggaag atgcagatga
     1681 tcttgatgaa aaagagatgg ccaatcgaat ggtgggtaga gagttaaatc agatgtttcc
     1741 tcctaaagta gaagctcaag gagaaaaagt tttagaagtc aaaaacttaa atgtgaaagg
     1801 tttattagaa gatattaatt ttgatctaaa aaaaggtgaa gtactgggtt tttccggttt
     1861 ggtcggagcg ggccggactg aattagcgga aactattata gggattagag aaaagacaag
     1921 cggagaaata tatataaacg gaaaaaaagt caacatagat tctccttctc aggctgttaa
     1981 aaatgacata gcctatctac cggaggatcg acagggggta ggtatactta ctaattttac
     2041 aatcccgagt aatatcacct taatatcatt aaaaaaatat actaaattat taatcaacca
     2101 taaaaaagtc aaagaaaagg caaagtatta tgtggatgag ttcaatattc aggctccttc
     2161 tatagatacc gaattagaat atttaagtgg cggtaaccag caaaaggttt ctttatcaaa
     2221 aagtttagat acagaaccta atatctttat ctttgatgaa ccgaccaggg gtattgatgt
     2281 aaatgctaag atggagatct ttaatttcat tcatgagtta gtacagtccg gtatttcggt
     2341 tatctttatc tcgtcagaat tagaagagat catcggaatg tgtacaagag tggtggttat
     2401 gagatcggga actatcacag gtatattaga gggtgagcat attaatgaag aagaaataat
     2461 gtatcatgct actggagtaa agagaggtgc tatctaacat tgaaagataa aaagaaaaaa
     2521 gaaggtaaaa acttctggga tttaatcaaa aatataaact ttgaaaagta tggaccttta
     2581 atagcattat tggtactttt tattatttct tcttttgcaa gtccctattt tttaaagttt
     2641 agaaacctga ccaatatttt aagacaggtt tcctatacgg gtattattgc cctaggtatg
     2701 acttttgtaa ttatctcggc cggtattgac ctatcagttg gttcaatgac agctttagtt
     2761 ggtggaattg taataatgac cttgaactat ttcggtgggg gcatttttgc aattttcatg
     2821 gcaatattag taggtatttt attcggatta gttttgggag cagttaacgg acttatcatt
     2881 acaaggtgga atgttgcccc cttcattgtc acgttgggaa cgatgtccat cttccgatca
     2941 ttaaccttat atattagtga tgccagtgaa tttagatcaa ccagtacaat attcccggaa
     3001 ctaggtatgg gctatttcct caagttaccg attccggtct ggatattttt tggattaacc
     3061 gtcctttata gtattgttct taaccatact agatacggcc gctatatctg tgctgtgggt
     3121 tccaatcaaa aagttgccaa atattcagct attaaggtaa agttgaccaa attttttgcc
     3181 tatgtaattg ttggtatcaa tgtcggtatt acagctgtcc tgttatcctc ccgtttaaat
     3241 tccattagtt cttcgggagc gggtacaggg tatgaactgg atgcaattgc tgctgtaatt
     3301 attggtggta ctgccatgtc aggtggaagt ggttcagtag taggaacttt gatcggagct
     3361 atcatattgg gaattgtaaa taacatgttg aatatgttgg gagtatctcc ttacctacag
     3421 gggacagtaa agggtctggt aattattggt gctgtattga ttcaaaggaa aaaagaatag
     3481 ttaaaatact caataagggg ggattttaaa aaagtctaat ttttcatatt aaaaaaggga
     3541 ggtaaaaaat taaaatgaga aaaatagctt taattttgac tctggtattg gtattttcat
     3601 taactctgtc tgctgttact tttgctcagg atgagattag aattggtgta acaattcctt
     3661 cggctgacca cggctggacc ggtggtgtag tgtattgggc acaacaggct attaaggact
     3721 gggaggcaaa gaacgataat atcacattct ttttagaaac tgctgattca cctgctaccc
     3781 aggtgagtgt tgttgaggac ttgatgatca aggaaataga tgctctagta attttagcac
     3841 atgattcagc tcccttaact ccggttgtaa aacaggcttc tgaagaaggc atctatgtag
     3901 tttctgttga tagaggttta acagaacctg ttgctgatat ctatgtcgcc ggtgacaacc
     3961 cgggtttagg tcgagtaagt ggagaatgga tggccaaaga attaaatggt gaaggagata
     4021 tagttgtgct agaaggtata ccctgtgtaa ttaataccca gagggtagag gcttttgaaa
     4081 aagtaatgga acagtatcct ggtattacta tcatggattc acagcccgcc aactggtcaa
     4141 ctcaaaaggg tcttgaaata atggaaaatt acttacaaaa atatgataaa attgatgcgg
     4201 tttgggccca ggatgacgat gtgctcaaag gtgtattaca ggcatatgaa gagtccggcc
     4261 gagatgatat taagttcttc ttgggtggag ccggttccaa agacatagtt aagatggtta
     4321 tggaaggtag tgatttggta agggcagatg taacatatcc accatccatg attgcaactg
     4381 gtattagtat ggcagttaaa gcaatgcagg gagaaacatt agatggattt tatcaaaatg
     4441 aactaccatc tgaagtaatc ttgagagctg aattaattac tgaagaaaat gcagaaaatt
     4501 actatttccc ggattcagta ttctaaacaa tatttaacat aaatagagag ggcacttagt
     4561 aatactaagt gcccctttcc acatctatat tatacagaat attataagaa aaataaacta
     4621 ataaaggaaa aaattatata aaaccacgct gtaaaggagg attattatgg gtgaaatatt
     4681 tcccgactta ccaaagataa agtatgaagg taaagaaagc ggtaacttat tatcttttaa
     4741 atactataac ccggaacaaa aaatcagggg taaaaagatg agggatattt taagatttgc
     4801 tgtagcatac tggcatacat tttgccatga tgggagtgac ccctttggag atgggaccct
     4861 acaaaagccc tgggttaata tccaagaccc tctacaaaga gctgaaaaaa aggcagccgg
     4921 tgcctttgag tttatggata aactgggggt tgactatttt tgtttccatg atagggatat
     4981 tgccccggcc ggacgcgatc taaaagaaac aaactataac ttagataaaa ttgtggagaa
     5041 gatcaaaggc ctgatggaag aaactaatat aaatctattg tgggggactg ctaatttatt
     5101 cagccatccc cgttatgcga atggggcagc tacctcctgt gatgccgata tttttgcata
     5161 tgcttcagct caagtcaaaa aggcgatgga agtcacaaat tatcttggcg gaaaaaacta
     5221 tgtattttgg ggtggaagag aaggttatga gaccctttta aatacagata tgaaacttga
     5281 acaggacaat atggcccgct ttttttctat ggcagttgaa catgccgaaa aaataggttt
     5341 taaaggacaa tttttaatag aacccaaacc gaaagaacca actaaacacc aatatgattt
     5401 cgatgttgcc aatgtaatag cttttttaag aaagtatgat ttagaacagt actttaaagt
     5461 aaatgcggaa gcaaatcacg ctacattagc tggtcataat tttcagcatg aactaaggtt
     5521 agccagaatt aacaaccttt tagggagtgt tgatattaat caaggagatt atttattagg
     5581 ttgggatacc gatcagtttc cctctaatat atatatgtca accttagcta tgtatgaaat
     5641 attaaaaaat gatggtataa gtccaggtgg tttaaacttt gatgctagag taagaagacc
     5701 ctccaaggat cctgtagatc ttttatatgg tcatattttg ggtatggaca gttatgcaaa
     5761 aggattaata attgcagata aaatgttaag agatagagtt tttgaggact ttattgataa
     5821 tagatatgta agctataatc gggatattgg acttaaaatt aaaaatggtc aagaaagttt
     5881 tgcttcactg gaagaatatg tattagaaaa agaaggagag tttaaaacaa gttctggtag
     5941 acaagagatg ttagaagcaa tattaaataa gtacatcctg gaaacaaatc taacttaagg
     6001 aaagaaggat aaaatatgtc ttatttaatg ggtatagata tcgggaccag taatgttaaa
     6061 attattgtaa tatcagctga aggagaactg gttgctattg aaaccagttc ctatcccctt
     6121 tactcacctc agccgggttg gtcagaacaa aatccaaccg actggtggaa ttcgacaaaa
     6181 gaggctctta aagcaataat agagaaaaat gatatcaata gtgatgagat taagggaata
     6241 agcctttccg gccaaatgca cagcctggtg atgctggatg aaaataaaga agtcataagg
     6301 ccggctattt tatggaatga cacgagaact acaagacagt gtgaggaaat ttatgaacaa
     6361 gtaggtggac tagcaaaatt aatagagctt gtttccaatc ccgccctgga aggctttacc
     6421 gctcctaagt tactctggat taaagagcat gaacctgcta attataaaaa aattagatat
     6481 attatgctgc cgaaagatta tataagatat aagttaactg atgaaattaa aagtgaagta
     6541 tcagataatg ctggtaccct actttttgat gttgaacata aaaaatggtc tgatgaaata
     6601 ttagaattgt taaatataag ttcagatata ttaccggagg ttctctattc tacagacata
     6661 gcgggatata ctactaaaga attagaatcc ctgatcggta tcaaagcagg aatccccctg
     6721 atagcagggg gagctgataa tgcctgtggg gcagtgggaa gtgggattat aaaagacggt
     6781 agggtaatgg tcagtattgg tacttcaggc gttgtgctgg cccaaagtga taaacctgtt
     6841 ccggatcaaa aaggaaggat ccatttattt aaccattcct atcccgataa gttttatatg
     6901 atgggggtta tgctctctgc agctggttcg tttaattggt tgaaagaaaa aatgtatgat
     6961 gacaaatata gtatcaaaaa attaaatgaa ttagctagaa attctaaacc gggtagcaat
     7021 gggttggtat ttttacctta tcttaatggt gaaaggaccc cccatgctga ttctaatgct
     7081 cgaggggtat acttcggttt gtctaataaa catgatataa gacatattgt aagaagtact
     7141 atggaagggg tggcttttgg tctaaaagat tcatttgggt taatcaaaag taaagatatt
     7201 aaagttgaac agataagagt aattggaggc ggggctaaaa gcagtctctg gtgtcaaata
     7261 ctatcagata tctttgaaca agaaatttcc cttattaata ttgaggaagg tcctgctttt
     7321 ggggcagctt taattgcagg tgtgggagct aatgtctttg ctagttttga aagtgcagag
     7381 gaaaaattta ttaaaattaa aaagacgata aaacctaata gtgataattt caaggtatat
     7441 aggcacaact atgaaagata taaaagttta tataacagct taaaagaaga ttttgccctc
     7501 cacaataaat aacttgctta tatccatttg tggtgtagtc acgggatgaa gataaaagat
     7561 atgaactata aaaaaggtca aaatatagtt ttgttcaata tagaaataaa ataataaaag
     7621 gggaatatta tgtttaaaaa agttactata attgcattaa tatttttaat aagcttttcc
     7681 tcaatagttt ttgctcaaga atgggacaca ttttctgaga aagatgatat gtccggagta
     7741 atgatatggt atgcaggttc acctaaaacc acatcaaata atccgatgag tactccttat
     7801 tataatggta caaaggcttc gttgcatgta ggtacagatg gctttagtga atggtgttat
     7861 atttctttta ataaagaacc cattattaaa aatacagaaa ttaaagattg ttataatgca
     7921 tttacaacta ggcttgaatg ggatgatgat atttgtcata tggactttac taaagaatgg
     7981 gggacatcat ttatacattt tgtagaagat aaaaaagcaa taaataaaat tatggaaaat
     8041 aacaaagtac ttttagaatt agattggtac ggagaaggta aagtgcattt tgaatttact
     8101 ttaaatagtt cagctgatgc tattaaaaaa atacgtgaga aaacaaatta aattaaaggc
     8161 tgttcattat agtggcttcc agagagtaga gaaagtcaca agataaaacc acatatcttg
     8221 tggctttttt aaataaaacc ttctaattct ttatatatag taactcaaag ccaatgttaa
     8281 gatctaaaat ataaaaggtg aggtaaaaat aatattataa aaagaaatca aatgcagttt
     8341 tttgcaatat aggatttagt atatgaagat catctactaa gagatatcga aagtgcaatt
     8401 gattttgact tcatatatga ttttgttgaa gacaagtaat ttctcaatct tgatcgtaca
     8461 agtatagacc cttttatact aagaaaaatt aattcattat ttatatgtta taagaggtat
     8521 actgcaaaca ataaaagaaa ttgcgggaaa cagcgctaat cgttaaaatt ttagcattag
     8581 acccttacaa gaaagtacct tacttttaca tactttaaac ttaatgggtt ggctatagat
     8641 gaagaacaga gagggtctat tagctctttc acgcttactt tttatttttc atctttttta
     8701 tttaattttc gaatcaatta aacaaaatca agcgttttaa tccctgttgt ctaatacgct
     8761 ttgtctacaa tagacctctg taaggtgatt ttcacatatt tttctataaa atcaatttaa
     8821 tttcaaagaa ataacaagga atctaataga caatctcaaa gtaaaaatat aaatagaata
     8881 atattttaga taattaaata gggtgattat atatgataga aaataaaaac aagggttttg
     8941 atgtggtt
//
LOCUS       DATGCJ010000166         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_648943, whole genome shotgun sequence.
ACCESSION   DATGCJ010000166 DATGCJ010000000
VERSION     DATGCJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648943"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1484
                     /locus_tag="VKN64_08105"
     CDS             135..1484
                     /locus_tag="VKN64_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HMA60241.1"
                     /translation="MSIFKAYDIRGVYNKDFDKETVYKIGYYLPDVVKAENALIGYDI
                     RKSSPEVFDYLTRGLIDRGIEVFSMGLATTPSVYFNTVDGDFDLSIQITASHNSKEYN
                     GMKISRQKALPVGYETGLDELEKLVNDSDLDVDKISSENMQDTVKEYHGQYKYLNFLK
                     EHQPNIDSLDIIVDASNGMSALFLNDIFDDEVDYINYEMNGDFPGHDPNPMKEENLTE
                     IRDNIDKKDYDVGVVYDGDGDRVIFLDENGRFISPDLITAILVDYFLKDDSDQYVLFD
                     IRTSRSVSEYIKKLGGKPYMWKVGHAFAKLKMRELDAVVGGELAGHYYFRDFYYCDSG
                     LLTSFIVLDVLNRYKKEEGIKLSDVIDKIDNYEGSGEINFQIDNKKEVMEKVVNYFKE
                     NDEPEQIYDFDGYRLEFEDWWFNVRPSNTEPYLRLVVEADNQELLAKKVTLLKDLIE"
     gene            1640..2182
                     /locus_tag="VKN64_08110"
     CDS             1640..2182
                     /locus_tag="VKN64_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60242.1"
                     /translation="MLYVSIITFTYFLAVPITIFVAIDGYKRKNKWLLWSLGTILSWP
                     LILPLYFSFRNLKKEEKRTGGLIWNIIRNYILIYSTNIFLVILLLLILPQNMGYLSNE
                     IFITEFAGFEISLSFIYYVIFIIWLIPSLLFLMIGSFFKDDYSKETGPTGKLKESRIK
                     KKEELSKKLDEFYEERQEES"
     gene            2319..2672
                     /locus_tag="VKN64_08115"
     CDS             2319..2672
                     /locus_tag="VKN64_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMA60243.1"
                     /translation="MSEKEFWEEREEMNKKIYEADNLEIKRFFNLDTNVYKEGALSTK
                     NKEMLGLASSMVLRCDDCIRYHIDRCYEEGVSKDEFFELFSVALIVGGSIVIPHLRRA
                     VKRLEELFEGEEEVK"
BASE COUNT          989 a          332 c          537 g          895 t
ORIGIN      
        1 agtatatata ctgtcgatag tggagaagac aatgtcaaat atataggtat cattgagcag
       61 tttttccagc ttaattaatt ctgcataata attatattgg agcttttaaa aactgatgct
      121 aagaggtggt ataaatgagt atctttaagg cttatgatat cagaggtgtc tataataaag
      181 acttcgataa agaaacagtc tacaagatcg gatattatct acctgatgtt gtgaaagctg
      241 agaatgccct gataggttat gatataagaa agtcttcacc tgaagtattt gattatctta
      301 caaggggttt gatcgataga ggtattgaag tcttttcgat gggattggcg accactccct
      361 ctgtatattt caatacggta gatggtgatt ttgacctttc aattcaaatt acggcttctc
      421 ataacagcaa ggaatataat ggaatgaaaa tttcccgcca aaaagcacta ccggttggtt
      481 atgaaacggg tctggatgag cttgaaaaat tagtaaacga ttctgattta gatgtagata
      541 aaatatcatc ggaaaatatg caggatactg tcaaagagta ccacggccag tataaatatt
      601 tgaatttttt aaaagagcac caaccaaata ttgacagttt ggatattata gttgatgctt
      661 caaatgggat gagtgcctta tttttaaatg atatatttga tgatgaagtt gattatatta
      721 attatgaaat gaacggtgat tttcccggtc atgaccccaa tccaatgaaa gaagaaaact
      781 taactgaaat aagggataat atcgataaga aagattatga tgtgggggtt gtatatgatg
      841 gtgatgggga ccgagtcatc tttttagatg aaaatggcag gtttatatca cctgacctca
      901 ttactgcaat tttagtagat tactttttaa aagatgatag tgaccagtat gtactcttcg
      961 atataaggac ttcccggtcg gtcagtgaat atataaagaa actgggcggc aaaccttata
     1021 tgtggaaggt tggacatgcc tttgccaagt taaaaatgag ggaattagat gcagtagtgg
     1081 gcggtgaact ggccggacat tattatttca gagacttcta ttattgtgat tcagggcttt
     1141 taacatcctt tatcgtttta gatgtgttaa atagatacaa aaaagaagag ggtattaagt
     1201 tatcagatgt gatagataaa atagataatt atgaaggctc cggagaaatc aacttccaga
     1261 tagataataa aaaagaggtt atggaaaaag tagttaacta cttcaaggaa aatgatgagc
     1321 cggagcaaat ttatgacttt gacggttatc gattagaatt tgaagactgg tggtttaatg
     1381 tcaggccttc taatacagaa ccctatttaa gattagttgt agaggcagat aatcaagagt
     1441 tacttgctaa aaaagttaca cttctcaaag atttaatcga ataattaaaa atataagggt
     1501 gcctttttag gcaccttttc agcttaaagt atattctttt aaattgaata aatatttaaa
     1561 atataatata ataagaatgg ttaaatatgg gttaaaaaat aattatatac atatttatgt
     1621 agaattgagg agataaatta tgttatatgt atcgataata acttttacat actttttagc
     1681 tgttcccatc accatttttg tggccattga tggttataaa cgtaagaaca aatggctgtt
     1741 atggtctttg ggaaccattc tttcctggcc tttaatttta cccctttatt tttctttccg
     1801 aaatcttaaa aaagaggaaa aaaggaccgg tggtttaatc tggaatatta ttagaaatta
     1861 tatattaatt tattcgacca atatattttt agtgatcctc ttactattga ttcttcctca
     1921 aaatatgggt tatctaagta atgaaatatt tattacagaa tttgctggat ttgagatttc
     1981 tttaagcttc atttattatg taatatttat tatctggtta ataccttctc ttttatttct
     2041 aatgatcggt tcctttttta aagatgatta tagtaaagag accggtccga caggaaaatt
     2101 aaaagaaagc aggattaaga aaaaggaaga actaagtaaa aaattagatg aattttatga
     2161 ggaaagacaa gaggaatcat gatatttaac aaacttataa ccaaaagatt tctatatttg
     2221 caaataacga ataatcatta tatacttaaa aaaatatcgg aaatgctatt taaataatgt
     2281 aaataatatt gaaatatagg ctagaggagg cataaattgt gagtgaaaaa gaattttggg
     2341 aagaaagaga agaaatgaat aaaaaaatat atgaagcgga taatttagag ataaaaagat
     2401 tttttaattt ggatacaaat gtatataaag aaggtgccct cagtacaaaa aataaagaga
     2461 tgttaggttt agcttcttct atggtcttaa gatgtgatga ttgtataaga taccatattg
     2521 accgttgcta tgaagaagga gttagcaaag atgaattttt tgaactgttt agtgtagctt
     2581 taatagttgg aggttcaatt gtaattcctc atctaagaag ggctgttaaa cgactagaag
     2641 aactatttga aggtgaggag gaagttaaat gattcaattt gaaccgatcg gaaaaataat
     2701 tactccctat aaagagcgta aaaatattcc ccggcagccc gatatggatg ctg
//
LOCUS       DATGCJ010000167         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_560090, whole genome shotgun sequence.
ACCESSION   DATGCJ010000167 DATGCJ010000000
VERSION     DATGCJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560090"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VKN64_08120"
     CDS             <1..289
                     /locus_tag="VKN64_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMA60244.1"
                     /translation="LILGCTHYPYLIPVIKKVMGEKFTLINPAETMAVKAKNILAVRG
                     MISGKDEDPVDFKEHEFYVSDLDKVSHKFLDKGTEYLGLPHLEFEELNIFA"
     gene            335..1114
                     /locus_tag="VKN64_08125"
     CDS             335..1114
                     /locus_tag="VKN64_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HMA60245.1"
                     /translation="MSEEGIIRSVQKSLKVLKYIMSSPGEVSTNELVEEFGYNRSTLH
                     HMLKTMKREGFLTQNQSSKNYNIGSEVFYGWMKDRDLREYLLRLRPVIEEIVDNCGET
                     ANIFIRENDKGVCLFGEEPEKVLKAHLIMGRKIPLFCTAVGKAMLAFMPKGKVERILK
                     ISDLKKYKENTIVDKEKLFNQLEEIKEKGYALEKEEYEDYINALGIPLINQYGDPVAA
                     VSIVGPVMRFTDEKIEECLPFILEKAREMSSILKGEYYKYI"
     gene            1299..2027
                     /gene="rph"
                     /locus_tag="VKN64_08130"
     CDS             1299..2027
                     /gene="rph"
                     /locus_tag="VKN64_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HMA60246.1"
                     /translation="MSNDRVDGRNNDEIRETEIERDFTKYAEGSVLVSTGDTKVICTV
                     SVEDSVPYFLRGQNKGWLTAEYSLLPRSTQTRTIREAAKGKRSGRTREIQRLIGRSLR
                     AIIDLEQIGERTLWVDCDVIQADGGTRTASITGAFVALYDAVQYMLENKMIEESPLKG
                     FAAATSVGIVDGEYLLDLCYEEDVNAQVDMNIVMDENGEIIEVQGTAEKDPFTREEMD
                     KFLDLAEKGILELIKKQKKSVGLK"
     gene            2028..2627
                     /locus_tag="VKN64_08135"
     CDS             2028..2627
                     /locus_tag="VKN64_08135"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HMA60247.1"
                     /translation="MTRKLFVASSNSGKITEIKDHFKELDFEIVTPACFDNMGEVIED
                     GDTLEENALKKARDRAKKTKLLTLADDTGLEVEYLNGKPGIHSARFAHEEATYKENND
                     KLLEVLKGVPYEKRRAQFRTVVAVVDPERNKEYTVSGYCKGYILDERRGDRGFGYDPL
                     FYVPEYDKTFAEMSVSLKNKISHRADALEKMKKVLKSRY"
     gene            2750..2826
                     /locus_tag="VKN64_08140"
     tRNA            2750..2826
                     /locus_tag="VKN64_08140"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2784..2786,aa:Gly,seq:tcc)
     gene            2836..2913
                     /locus_tag="VKN64_08145"
     tRNA            2836..2913
                     /locus_tag="VKN64_08145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2871..2873,aa:Arg,seq:tct)
     gene            2924..3000
                     /locus_tag="VKN64_08150"
     tRNA            2924..3000
                     /locus_tag="VKN64_08150"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2958..2960,aa:His,seq:gtg)
BASE COUNT         1135 a          413 c          667 g          906 t
ORIGIN      
        1 tttgatttta ggttgtactc attaccctta tttgattcct gttattaaaa aagtgatggg
       61 agagaagttt actttaataa atccagctga aacaatggcg gtaaaagcta aaaacatttt
      121 agccgtacga ggcatgataa gcggcaagga tgaagatccc gttgatttta aagaacatga
      181 attttatgta agtgatttag ataaggtatc ccataaattt ttagataaag gcacagaata
      241 tttaggactt cctcatttag agtttgaaga actaaatatt tttgcttgaa aaaaaataat
      301 aaaatcatta tttttaaaaa gggagatgac ttatatgagt gaagaaggta ttattaggtc
      361 agtacagaaa agcttgaagg ttttaaagta cataatgtca tctccgggtg aagtttctac
      421 aaatgaacta gtggaagagt ttggctataa tcgcagcacc ctgcaccaca tgttaaaaac
      481 aatgaaaaga gaagggtttt tgactcaaaa ccaaagtagt aaaaattaca atattggctc
      541 agaagtattt tacggttgga tgaaagatag ggatttacgt gaatatcttt taagacttag
      601 acctgttatt gaagagatcg ttgataactg tggtgaaaca gccaatattt ttattagaga
      661 aaatgataaa ggggtttgtc tttttggaga agaaccggaa aaagtattaa aggcccatct
      721 tataatggga agaaagatac cattattttg tacagccgta ggtaaagcga tgttagcttt
      781 tatgccaaaa gggaaagtag aaaggattct taaaatatcg gacttaaaga aatacaaaga
      841 aaataccatc gttgataaag agaagttatt taatcaatta gaagagataa aagaaaaagg
      901 atatgcatta gaaaaggaag aatatgaaga ttatattaat gcattgggaa tacctttaat
      961 caatcaatat ggagatcccg tggctgcggt ttcgatagta ggacctgtta tgcgattcac
     1021 tgatgaaaag atagaggaat gtttaccatt tattttagaa aaagccaggg aaatgagcag
     1081 tattttaaaa ggagaatatt ataaatatat ttaaccttta cagccccatt tggggttttc
     1141 tttttatgat atgtgtttta agtatattga attgactata tatttaatac cttatagcat
     1201 aatattttat taaattcgat gttttttctt gttttgactt taggatttaa atcctgtaaa
     1261 attaggatac aaagatatat gtacttagga ggaataatat gagcaatgac agagtagatg
     1321 gacgcaataa tgatgaaata agagagacag aaattgaaag agattttact aagtatgcag
     1381 aaggatcagt tttagtttcc acgggagaca ccaaagtaat atgtactgtt tcagtagaag
     1441 atagtgtacc atatttttta agaggccaaa ataaggggtg gttaaccgca gaatactcac
     1501 ttttacccag atctactcaa actagaacaa taagagaggc agctaaaggt aaaagaagcg
     1561 gccgcactcg tgaaatacaa agattaatag gtcgttcatt aagggcaatt attgacctag
     1621 agcagattgg agaaagaact ctctgggtgg actgtgatgt tattcaagct gatgggggaa
     1681 ccaggacagc ttccattaca ggtgcctttg ttgccctata tgatgcagtt caatatatgt
     1741 tagaaaataa aatgatagaa gaatcacctt taaaaggatt tgctgcagcc actagtgttg
     1801 ggatagtaga cggagaatac ttactggact tatgttatga agaagatgta aatgcccagg
     1861 tagatatgaa tatagtcatg gatgaaaatg gagaaattat tgaagttcag ggtactgctg
     1921 aaaaagatcc ctttactaga gaagaaatgg ataaattctt agatttggct gaaaaaggta
     1981 ttttggaatt aataaaaaaa caaaagaaaa gtgtggggtt aaaatagatg acaagaaaac
     2041 tatttgtagc aagttctaat tctggtaaga taactgaaat taaggaccac tttaaagaat
     2101 tagactttga aattgtaact ccggcctgtt ttgataatat gggagaggtt attgaagatg
     2161 gagatacttt agaagagaat gctctaaaaa aggcaagaga ccgggctaaa aaaacaaagc
     2221 tattaacatt agcagatgat acaggacttg aagtcgaata tctaaatggg aagccaggta
     2281 ttcattctgc acgttttgcc catgaagaag ctacttataa ggaaaataat gataaactac
     2341 tagaggtgtt aaagggagtt ccatatgaaa aaaggagagc ccaatttaga actgtagtag
     2401 cagtggtaga tcctgaaaga aataaggaat ataccgtttc aggttactgt aaaggatata
     2461 tattagatga aagaagagga gatagaggtt ttggatatga tcccctcttt tatgtaccag
     2521 aatatgataa gacttttgct gaaatgtcag taagtttaaa gaataaaatc agtcatagag
     2581 ccgatgcatt agaaaaaatg aaaaaggtat taaaaagtag atattaggag tattatatac
     2641 ttaaacagtt cttatcatat ataattaaga ttatttaata gtagaaaatt ttgaccaaag
     2701 cgtcaattta agttatacta atacttgtcg caagaaaaaa acactgtgag cgggagtagc
     2761 tcagttggct agagcatcag ccttccaagc tgagggtcgc gggttcgagc cccgtttccc
     2821 gctccataat aatatgcgcc tgtagctcag tctggataga gcaacggact tctaatccgt
     2881 aggtcagagg ttcgaatcct ctcaggcgta ccattctaac atggtgggta tagctcagct
     2941 ggctagagcg caggattgtg gctcctgagg tcgtgggttc aagacccatt acccacccca
     3001 ttttttattc taaacataat ttttatttaa aaatagatac ggaattatca cttaagtaga
     3061 ccattagctc aatcggaaga gcaactgact cttaatcagt aggttcgggg ttcgagtccc
     3121 c
//
LOCUS       DATGCJ010000168         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_335701, whole genome shotgun sequence.
ACCESSION   DATGCJ010000168 DATGCJ010000000
VERSION     DATGCJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335701"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /gene="pheT"
                     /locus_tag="VKN64_08155"
     CDS             <1..1507
                     /gene="pheT"
                     /locus_tag="VKN64_08155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMA60248.1"
                     /translation="QETLVITDPDKVITVNGVMGAENSEVTDQTTDIFLEAAYFEPTS
                     IRKTARKLGLPSEASHRFERGIDIERVIEAGNRAAYLMQEYADGKVAQGIIDKYPVPY
                     QENIIDVNLAKVNRLLGIELNKDEIEEMLKRLEIEVIEKEPESIKVKAPSYRNDIEQD
                     ADIIEEIARIYGYNNIPSTVPKTNEVGKRSRKQKIEKEIKNTMNAAGLDEIITFSLTG
                     PEDYKNLGLSEDHRYKKWVSIKNPLNEAFGLLRTSLLPNLIDSISTNAKRQIDQVSYF
                     EIGRIFLNQGHNERPEEKTKLAAGSMGAGEDIWELDAPDFYYLKGALEKLFDQLDISK
                     EIEFVRGKEDFMHPGRTAEIRFKNERIGILGELKPQVIDSYDLKERTAVAEIDLNKIL
                     TEVEIGDYLFEMLPKYPAVARDLAVLISEEVPVMEVLNLINSTDDSILKKTELFDIYQ
                     GEQIPAGQKSIAFKLLFQAPDRTLTDEEVNEVFNQIVKNLEDKYEAEIRKK"
     gene            1583..1927
                     /locus_tag="VKN64_08160"
     CDS             1583..1927
                     /locus_tag="VKN64_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMA60249.1"
                     /translation="MEKYKEENKFTVKILDEELVVKGKVSEDYVRDLAEYINNVGQEI
                     QETYPRLPRRSLLGLTMINIADDLFKTKSELQNLKEENEDLKEVRDKLIKDNNSLKRD
                     NRELNNLLEEVD"
     gene            1931..2428
                     /locus_tag="VKN64_08165"
     CDS             1931..2428
                     /locus_tag="VKN64_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMA60250.1"
                     /translation="MDGSITVIDIVISIILLYFIFEGYKKGFVKQTATILGILVSLYL
                     AITRYAEFQYFLKPYLNVSETFLQFISFAVIFIGVNIVIQILSNVLEKMTDNAFVKPV
                     NQVGGALFGLLKGGVLVYFLLLILSEIPYQSLTDTIDQSMFADRFLDLTPYVKENLER
                     LFGHN"
     gene            2463..4193
                     /gene="polX"
                     /locus_tag="VKN64_08170"
     CDS             2463..4193
                     /gene="polX"
                     /locus_tag="VKN64_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HMA60251.1"
                     /translation="MHSFSNKEISEMLSEYTDLMKIMGENKYRVRAYDNASRIVSDRT
                     IDLEKMVKEDRLEEIKGIGEGIAGTIKDIFEKSYCEPLEDLRKELPPGFEELLNIPGL
                     GPKTAGKFLEELEISNIQELKKALKEEKIRELKGMGAKTEENLLEALKEYEEYTSFIN
                     LDQALETSTKLINELKEKSDEIIDIETAGSTRRRKIKIGDLDILMSVKDKKDHNLLKT
                     IKELEPIKDILLEGDTKISGRTQDGLQVDFRIVNQDEYPAALLYFTGSKYHNVKLRQI
                     AKDKNYKLNEYGVFEGDKRIEASSEKEIYGLLDLKYMEPEIREDRGEIEAAQKDELPD
                     LITIDDIKGDFHVHSNYSDGAMSIREMVEAAQERGYQALAFTDHSQSLNIANGLSEER
                     VKEQWQEIEEVRKEYPDFLILKGIEVDIKKDGSLDYTDDILDGFDLVIASVHANFNLP
                     KAEMTERIIKAIESPYVNIIGHPTGRMLGQRQPFEFDFEEVLKKAKENNVILEINASP
                     SRFDLNDAMARDALREGVKLIINTDSHHSDQYDYMKYGICIARRAWAEKEDIVNTYSI
                     KELKELFEVK"
     gene            4195..>4516
                     /locus_tag="VKN64_08175"
     CDS             4195..>4516
                     /locus_tag="VKN64_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HMA60252.1"
                     /translation="MDKHSIKVLELAKIMDDVKEQADTFLGQKLVSELKPYKDLDYIK
                     ERLKEVTQAKRIYNDYSKPPFGGVRDIREFLKKSDKEVVLSIKELIDIESTLNAFRNM
                     RGFFN"
BASE COUNT         1584 a          769 c         1001 g         1162 t
ORIGIN      
        1 tcaggaaacc ctggtcatta ccgatcccga caaggttatc acagtgaacg gtgtgatggg
       61 ggctgaaaat agtgaggtaa ctgatcagac taccgatatc tttttagaag cagcctactt
      121 cgaaccgacc agtatcagaa agaccgcccg caagctgggc ctgcccagtg aggcctctca
      181 ccgttttgaa cgcggtatag atattgaacg tgtaatcgag gccggtaacc gggccgccta
      241 cctgatgcag gagtatgcag atggtaaggt agcccaagga attattgata aatatcccgt
      301 tccttatcag gaaaatatta tagatgttaa cctggccaag gttaaccgct tactggggat
      361 cgagttaaat aaagatgaaa ttgaagagat gttaaaaaga ttggagatag aagtaatcga
      421 aaaagaaccc gaatcaatta aagtgaaagc accttcttac cgtaatgata tcgaacagga
      481 tgctgatatc atagaagaga ttgcccgtat ttacggttat aataatatac cttcaactgt
      541 ccctaaaacc aatgaagttg gtaaaagaag ccgcaaacaa aagattgaaa aagagatcaa
      601 aaatacgatg aatgcggccg gcctagatga aatcattacc ttcagtctaa ccggaccaga
      661 agattataag aacctaggac tctcagaaga tcacagatat aagaagtggg ttagtattaa
      721 aaacccctta aatgaagcct ttggactgtt aaggacttca cttttaccga acttgatcga
      781 ttctatctcg accaatgcca aaagacagat cgatcaggta agctactttg agatcggccg
      841 gatatttctt aaccagggtc ataatgaaag gccggaggag aagacaaaat tagcggccgg
      901 ttcgatggga gccggagaag atatctggga acttgatgcc cccgacttct actatctgaa
      961 aggtgcatta gaaaaacttt ttgaccaact tgatatcagt aaagagatcg agtttgtacg
     1021 gggtaaagaa gacttcatgc accccggtcg gaccgctgaa atcaggttca aaaatgaaag
     1081 gatcggtatc ttaggagagc ttaaaccaca agttatcgac agctatgatc taaaagagag
     1141 aacagccgtg gctgagattg atttaaacaa gatcctaacg gaagttgaaa tcggtgacta
     1201 cctctttgaa atgctgccta aatatccagc tgtagcgcgt gatttagcgg tcttaatttc
     1261 agaagaggta ccggtcatgg aagtattgaa cctaatcaat agcactgatg attctatctt
     1321 aaagaaaact gaactctttg atatctatca gggtgaacag atcccggccg gtcagaaatc
     1381 aattgctttt aaattactat tccaggcccc ggaccgaacc ttaaccgatg aagaggtaaa
     1441 tgaagtattc aatcagatag ttaaaaactt agaagacaaa tacgaggcag aaataagaaa
     1501 aaaataggtg cagttgcagg aatttgaaat ataatagtga aattattaat gtagaacata
     1561 aacaaacagg ggctgtataa ttatggaaaa atataaggaa gaaaataaat tcacggtaaa
     1621 gatcttggat gaggaactgg tagttaaggg taaggtttca gaggactatg tcagggatct
     1681 ggccgaatat ataaacaatg tgggccagga gatccaggaa acctatcccc ggctcccgcg
     1741 caggagtcta ttaggtctga ccatgatcaa tattgccgat gatttattta aaacaaaatc
     1801 tgaactccag aacttaaaag aagaaaatga ggacctgaaa gaagtaagag ataaattaat
     1861 taaagacaat aattcactga agcgcgacaa tagagaatta aataatctac tggaggaggt
     1921 agattaataa ttggatggct caattactgt tatagatatc gtgataagta tcatcctatt
     1981 atatttcata tttgaaggct ataaaaaggg atttgtaaaa cagactgcca caatcttagg
     2041 tatcttagtt tccctttacc ttgcgatcac aaggtatgcc gagtttcaat actttctcaa
     2101 accatattta aatgtatcag aaacttttct gcagtttatt agttttgcag taattttcat
     2161 cggagttaat atcgttatcc agattctaag caatgtcttg gaaaagatga cggataacgc
     2221 ctttgtcaaa cccgtcaacc aggtgggagg tgctctgttt ggacttttaa agggaggagt
     2281 attagtctac tttctgcttt taatcctttc agagattccc taccagtctt tgacggatac
     2341 tatagatcag tcgatgtttg ctgatagatt tttagattta actccctatg taaaagagaa
     2401 tctagaacgt ctttttggcc ataactaatc ttatggtcaa tttttttgta aggggtgaaa
     2461 aaatgcacag cttcagcaat aaagaaatat cagagatgct aagtgaatat accgacttaa
     2521 tgaagatcat gggagaaaac aaataccggg taagagccta tgataacgcc tcccggattg
     2581 tgtccgaccg tacgatagat ttagaaaaga tggtcaagga agaccgctta gaagagatca
     2641 agggtattgg agagggaatt gccgggacta ttaaagatat cttcgaaaag agctactgtg
     2701 agcctttaga agatttaaga aaagaactac cgcccggttt tgaagaactg ctcaatatcc
     2761 cgggtttagg tcccaaaaca gccggtaagt tcttagaaga gttagaaatt agtaatattc
     2821 aagaactaaa aaaggcttta aaagaagaaa agataagaga gcttaaaggt atgggggcta
     2881 agacagaaga aaatctctta gaggccctaa aagaatatga agagtatacc agtttcatca
     2941 atctggatca ggccttagaa acttctacta agttgataaa tgaactgaaa gagaaatcag
     3001 atgagataat cgatatcgaa accgccggtt ctaccagaag aaggaagatt aagatcgggg
     3061 acctcgatat cttgatgtct gttaaggata aaaaagacca taatctatta aaaacgatca
     3121 aagaattaga acctataaaa gatatcctgt tagaagggga taccaagatt tcaggccgca
     3181 ctcaagacgg cttacaggtc gattttcgga tcgtaaatca agatgaatat ccagctgcct
     3241 tactctactt taccggttct aagtaccata atgttaagtt aagacagatc gccaaggata
     3301 agaactacaa gttaaacgaa tacggtgtat tcgaaggaga taagagaatt gaggcctcct
     3361 ctgaaaagga aatatatgga ctgctggact taaagtacat ggaaccagaa attagggaag
     3421 accggggcga gatagaagca gctcaaaagg atgagctgcc ggacctaatt accatagatg
     3481 atattaaggg agacttccac gtccattcta attacagtga tggggcgatg agtatcagag
     3541 agatggtgga agctgcccag gagagaggtt atcaggccct ggcctttaca gatcattctc
     3601 agtccttaaa tattgcaaat ggactctccg aagaaagggt aaaagaacag tggcaggaaa
     3661 ttgaagaagt aagaaaagag tatcccgact ttctaatctt aaaagggatc gaagttgata
     3721 tcaaaaagga cggtagtctt gactatacag acgatatttt agacggcttt gatcttgtga
     3781 ttgcttctgt ccatgctaac tttaacttac ccaaggctga gatgacagaa cgaattatta
     3841 aggcgatcga aagtccctat gttaatatta tcggccaccc gactggccgg atgttaggac
     3901 agcggcagcc ttttgaattt gactttgaag aagtcttaaa aaaagccaaa gaaaataatg
     3961 ttatacttga aataaatgca tctccttccc gttttgatct aaatgacgcg atggcccgtg
     4021 atgccttacg ggaaggtgtt aagttaatta taaataccga ttcacatcac agtgatcagt
     4081 atgattacat gaagtacggg atctgtatag cccgtcgggc ctgggctgaa aaagaagata
     4141 tagtaaacac atattccata aaagagttaa aagaactatt tgaggtgaaa tagattggac
     4201 aaacatagta ttaaagtttt agaactggcc aagataatgg atgatgtaaa agaacaggcc
     4261 gataccttcc tcggccaaaa gttagtctcg gagttaaaac catataagga cttagactat
     4321 attaaagaaa gattaaaaga agttacccag gctaaaagga tatataatga ctatagtaag
     4381 cctccttttg ggggagtaag agatattagg gagtttttaa aaaaatctga taaagaagtt
     4441 gttttatcaa ttaaagaact aatcgatatt gaaagtactt taaatgcctt tcggaacatg
     4501 cggggtttct tcaatg
//
LOCUS       DATGCJ010000169         6088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_335753, whole genome shotgun sequence.
ACCESSION   DATGCJ010000169 DATGCJ010000000
VERSION     DATGCJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335753"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VKN64_08180"
     CDS             complement(<1..590)
                     /locus_tag="VKN64_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA60253.1"
                     /translation="MLTKSPAEARIIKLLKNCTYLDRKFNIETGDILIEDGIIIDIDK
                     NIEKEKSQLEGSMDCKDYLILPGFVNGHTHNPSTLYRGLFRDKPLSDWLDNSYQGQLQ
                     NKGFEFLDDKVNREEFHVLLLKSYSEYMRQGISTVVESGQSDREDVINWTEDVLGTTK
                     LRGVIDVYDEIDNYYNNSDEYVKYCSHLPEEELIDDT"
     gene            complement(593..1117)
                     /locus_tag="VKN64_08185"
     CDS             complement(593..1117)
                     /locus_tag="VKN64_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HMA60254.1"
                     /translation="MDKRKVEVKEHKKEWNTLYEKEKIELTDIFKDILKDIHHIGSTA
                     IPNIKAKPVIDIMVVVQDINKVDQYNEEMIELGYEPKGEFGIKNRRFFQKGGSNRTHH
                     VHVFPKGDKEVQRHLNFRDYMRAHPQKARQYSQLKETLAAQYPHDIDKYIEGKNDFIK
                     EIDREAAKWAADDH"
     gene            complement(1135..1971)
                     /locus_tag="VKN64_08190"
     CDS             complement(1135..1971)
                     /locus_tag="VKN64_08190"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HMA60255.1"
                     /translation="MEASELKNTLQEIADNEYNLLENIDYEELTNEMLAKIGVTDPEL
                     RDDLIYMVMANWIMDDIYNKEELRQIFNICLDEEHLYYKLGEKEDDSVFTRTFSALIL
                     AVLYHKDYEDDFLNQQEFQQGAETVIDYYTEEKDLRGYIKGKGWAHSAAHGADILLEI
                     AKSKKAKKRILMKLLNAIIDKSQIEHHVYFNGEDERIARAVKSTMKNINLDDETINKW
                     FRDLTDIESFEDRNKQDTLIFNLKNILKSLYFEIISDEVVNDKYLILITDALKELDQK
                     RF"
     gene            complement(1995..2660)
                     /locus_tag="VKN64_08195"
     CDS             complement(1995..2660)
                     /locus_tag="VKN64_08195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60256.1"
                     /translation="MTDNFERMDEFFDKRATSYDNHMQVNVNDFENFYIKIAEPIKRT
                     DEKISILDIGCGTGLELEYVFKKAPRANITCLDLSEKMLTKLEEKFEDKKAQIETRVG
                     SYLDNNLGTKQYDYIISVMTVHHLKYGKKLKLYKDIKRALKKGGRYIEGDYVVSRQKE
                     KKLLKEYEIKTASLEGADQGNYHIDIPFSLKTQKKLFIKTGFKDFHLIYKKGEAAIYV
                     MQN"
     gene            complement(2744..3796)
                     /locus_tag="VKN64_08200"
     CDS             complement(2744..3796)
                     /locus_tag="VKN64_08200"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HMA60257.1"
                     /translation="MRLVPVEDIKPDMKLARSIRHKDNVLLSTGVKNLHRYKAKLKNI
                     GINYVYIEDKYSADIKIDYVIKDELREKGQSRIRSFMEDIKFNKSINVGQIKDTVADI
                     VDDILNNSDNIIVNMLDIKNYDDYTYEHSVNVAVYTIILGKSLNYSRNDLNKLGMGAI
                     MHDVGKILIPPKILFKEDKLTNEEYEIVKEHSELGYDYIKDTREISQVSKEAILSHHE
                     RLDGSGYPRNLKDEEIHEFAKIVAVTDVYDALTSDRTYRKRWPTHEAISYIMSNSDQK
                     FDNYFVEKFIRNLAIYPNGTMVKLSDGRKAIVKEQNGNYPTRPVLRVIEDAEGNEYIE
                     DLDLLQNLNVVIEEVY"
     gene            complement(4005..4490)
                     /locus_tag="VKN64_08205"
     CDS             complement(4005..4490)
                     /locus_tag="VKN64_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HMA60258.1"
                     /translation="MIKTAVVTISDKGSQGKRKDKSGPVIIEMLKEFEAEKSYYNIIP
                     DELEEIKEELIKLSNKESIDLILTTGGTGFAPRDVTPEATQAIMEKDAPGIADLMRMD
                     TISITKKAALSRAVSVIRNDTLIINLPGSPKAVRECLQAVKDIIPHGIEILKGEVTEH
                     N"
     gene            complement(4505..>6088)
                     /locus_tag="VKN64_08210"
     CDS             complement(4505..>6088)
                     /locus_tag="VKN64_08210"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HMA60259.1"
                     /translation="VEKINGKRVEVIKSVPVWNNIRSIGESVVKQQMIVPSHKEVNVY
                     EVGAMLEAGVTELEVYQNLKVSIIPTGSELVKPAQKKEKGQLVDFNTAMLKLATQNLG
                     CTVHDFDIVPDDYEQIKRAVLKAVADSDITIINAGSSAGKEDYTLSILQELGEVFVHG
                     VDIMPGKPLIIAEVDEKAVIGLPGYPLSALLDFHLFVEPLVKKMLGLNYELPDKVKAE
                     VKKKLPSTAGLKEFIRVNLAEIDGQLLALPQKRGSATMQSLINADGILPIPESKEGIL
                     AGEEADVYLLKTRNTIKKNLMMVGSHDLTIDLIRDLVKKNKYDFELSIQSVGSLSGLV
                     SLKRGECHLAGAHLLDTETGKYNTSYIKDLLKGEEIAVINLVHREQGLLVKKDNPKDI
                     KGIRDLTRDDIKYINRQRGSGTRVLLDYLLHKEDINIGDIDGYEKEELTHMGVAVQVA
                     EGNADTALGIRAAAEAVDIDFIPVKKEKYDLILKKKDLNDARIDKIIDTIRSDEFKEK
                     VNRLGGYDTKESGYINFIE"
BASE COUNT         1630 a         1271 c          889 g         2298 t
ORIGIN      
        1 gtgtcatcaa ttagttcttc ttccggcagg tgagaacagt atttaacgta ttcatctgag
       61 ttattataat aattatctat ttcatcatat acatcaataa cccctcttaa cttagtggtt
      121 cctaagacat cttcagtcca gtttattaca tcctctctat cagattggcc gctttcaact
      181 accgtagaaa tcccctgtct catatattca gagtaggact taagtaagag gacatgaaac
      241 tcttcccggt ttactttatc atctaagaat tcaaaacctt tattttgcag ctgtccctgg
      301 tagctattat ccagccagtc tgaaagcggt ttatccctga aaagacccct atataaagtg
      361 gaagggttat gggtatggcc gtttacaaaa ccgggcaata taaggtaatc cttacagtcc
      421 attgatcctt ctaattggga cttttctttt tcaatgtttt tatcaatatc tattatgatc
      481 ccatcttcaa ttaatatatc tcctgtttca atattaaatt ttctatctag ataagtgcaa
      541 ttttttaata acttaattat tctcgcctcc gctggacttt ttgtcagcat ttctagtggt
      601 catcggcagc ccattttgct gcctctctat ctatttcttt aatgaagtca tttttccctt
      661 caatatattt atcaatatca tgaggatatt gggcagctag tgtttctttt aactgtgaat
      721 actgcctggc cttctgggga tgggcccgca tgtagtcgcg gaaattaagg tgtctttgta
      781 cttctttgtc gcccttggga aagacatgga catggtgagt tctattacta ccgccctttt
      841 gaaaaaaacg ccggtttttt atcccgaact cacctttggg ttcataacca agttcaatca
      901 tttcttcgtt atattgatct accttattta tatcttgcac tacgaccatg atatcaataa
      961 ccggttttgc ttttatatta gggatagcag tacttcctat atggtggata tcttttaata
     1021 tatctttaaa tatatcggtt agttctattt tttctttttc atataaggta ttccattctt
     1081 ttttatgttc tttaacttct acctttctct tatccaagtt aaacacatcc ttaattaaaa
     1141 tcttttttgg tctaattcct ttaaggcatc cgtaattaaa attaggtact tatcattaac
     1201 aacttcatct gaaatgatct caaaatagag gctttttaag atatttttta aattaaaaat
     1261 caaagtatcc tgtttatttc tatcttcaaa actttcgata tctgttaaat ctctaaacca
     1321 cttattaatt gtttcatcat ctagattaat atttttcatg gtacttttaa ccgcccgtgc
     1381 gatcctttca tcttcaccat taaaatagac atggtgttct atttgtgatt tatcgataat
     1441 ggcattaagt aatttcatca atattctttt cttcgctttt ttacttttgg cgatctctaa
     1501 taatatatcg gctccatggg cggccgaatg ggcccagcct tttcccttaa tataacctct
     1561 caggtctttt tcctcagtat aatagtctat tactgtttcg gctccttgtt ggaattcctg
     1621 ttggtttaaa aaatcatctt cataatcttt gtggtacaaa acggccaaga ttaaagctga
     1681 aaaagtcctg gtgaaaactg aatcatcttc tttttctcct aacttataat agagatgctc
     1741 ttcatccagg cagatgttaa agatctgtct tagttcttct ttattataga tatcatccat
     1801 tatccagttg gccattacca tgtagatgag gtcatctctt aattcagggt cggttacccc
     1861 tattttagct agcatttcat tggtaagttc ttcataatca atattttcta ataggttgta
     1921 ctcattatcg gcaatctctt gtaaggtatt ttttaattca cttgcttcca tcgattaacc
     1981 tcctaattgt tgtattaatt ttgcatgaca tagatggctg cttcaccttt tttatagatt
     2041 aaatgaaaat ccttgaatcc ggttttgata aaaagtttct tctgggtctt aagtgaaaac
     2101 ggaatatcaa tatggtaatt gccctgatca gcgccctcta aactggctgt tttaatctca
     2161 tattctttta atagcttttt ttctttttgt cggctgacta cgtaatctcc ttctatatat
     2221 cggccgccct tttttaaagc tcttttaata tctttatata actttaattt tttaccatac
     2281 tttaaatgat gaaccgtcat tactgaaata atataatcat actgctttgt acccagatta
     2341 ttgtctaaat aagaaccaac tctcgtctct atctgagctt ttttatcttc aaatttttct
     2401 tctaatttag taagcatctt ctccgataag tccaggcagg ttatattggc cctcggagct
     2461 tttttgaata catactccag ttcaagcccg gtcccacagc cgatatccag gattgatatc
     2521 ttttcatcag tcctttttat gggctcagca atcttaatgt aaaagttttc aaaatcattg
     2581 acatttacct gcatatggtt atcataactg gtagcccttt tatcaaaaaa ctcatccatt
     2641 ctttcaaaat tatctgtcaa ggtgtttctt cctttctgct tatatattta taatattcaa
     2701 aaaattaagg tccgcagata ttaatgcttt atttatagtt aatttaatat acttcttcga
     2761 tcaccacatt gagattctgc aggaggtcga gatcttcgat gtattcattg ccctcggcat
     2821 cctcaataac tctcaggacc gggcgagtag gataattacc gttctgttct tttacaatgg
     2881 ctttccgtcc atcgcttaac ttaaccatcg taccgttggg ataaatagct aggtttctga
     2941 taaatttttc tacgaagtaa ttgtcaaact tctgatctga gttactcatt atataactta
     3001 tggcttcatg ggtcggccat cttttacggt aggtcctgtc acttgttagg gcatcatata
     3061 catctgttac agccactatc ttggcaaatt catgtatctc ttcatctttt aaattcctcg
     3121 gatagcctga accgtctaat ctttcatggt gggataaaat agcctcttta gaaacttgac
     3181 taatttcgcg agtatctttt atataatcat aacctaattc agaatgctct ttaactattt
     3241 catattcctc attggttaat ttatcttctt taaataagat cttgggggga attaatatct
     3301 tacccacatc atgcataata gctcccatac ctaatttatt aagatcattt ctgctatagt
     3361 taagtgattt acctaatatt atcgtataaa ccgcaacatt gacagaatgt tcataggtgt
     3421 agtcatcgta attctttata tcgagcatgt taactattat attatctgaa ttatttaaga
     3481 tgtcatctac tatatcggcg accgtatctt ttatttggcc tacatttatt gacttattga
     3541 acttgatatc ttccataaaa ctcctgatcc ggctctggcc tttttctctt aattcatctt
     3601 tgatcacata atcaatttta atatccgcag aatacttgtc ttctatatag acatagttta
     3661 ttcctatatt ttttaatttt gctttatagc gatgtaagtt tttgactcct gtgctcaaaa
     3721 gaacattgtc cttgtggcgg attgatctcg ccaatttcat gtcaggctta atatcttcta
     3781 ccggtaccaa cctcattatt tgatcacctt tcctatattg taaattatta ttaaatttgc
     3841 tcctattatt aataatttac tgaactatat aatatatctt taataggata atttaccaac
     3901 taataaaatt aacattgtca aaatttgaaa atatttgtat cattaggtaa gactaactta
     3961 tattatataa tattaaagaa aataataaaa gagcataatt aagattaatt atgctctgtg
     4021 acctcacctt tcagtatttc tatcccgtgc ggaattatat cttttactgc ttgcaaacat
     4081 tcccgtaccg ccttgggact accgggcaga ttaattatta aagtatcgtt tcttatgacg
     4141 gaaaccgctc ttgataaggc ggcttttttt gtgatactaa tagtatccat tctcattaaa
     4201 tctgctatac caggtgcgtc tttttccatt atagcctggg tggcttctgg ggttacatca
     4261 cgtggtgcaa aacccgtacc ccctgtagtt aaaatcaaat ctattgactc tttatttgat
     4321 aatttaatta actcttcttt tatttcctct aattcatccg gaataatatt gtagtaactt
     4381 ttttcagctt caaattcttt taacatctct ataatgacag gaccgctctt atcttttctt
     4441 ttaccctggg aacccttgtc actgatcgtt actaccgccg tcttgatcat tttatctccc
     4501 ctttttattc tataaaatta atataaccgc tttctttggt atcatagccg cctaatcgat
     4561 ttaccttttc tttaaattcg tcagaacgga ttgtatcgat gattttatct attcttgcat
     4621 catttaggtc ctttttcttt aatattaaat catacttttc tttttttaca ggtatgaaat
     4681 caatatcgac cgcttcggcc gctgccctaa tccctaaggc tgtatcggca ttaccttccg
     4741 ccacctggac cgctaccccc atgtgggtta attcttcttt ctcataacca tctatatcac
     4801 ctatattaat atcttctttg tgcagtaggt aatctaataa tactctggtg cccgaacccc
     4861 tctgccggtt aatatactta atatcatctc tggttaaatc tcttattccc ttgatatctt
     4921 tggggttgtc ttttttaacc agcaggccct gttcccggtg cacgagattg ataacagcaa
     4981 tttcttctcc cttgagaagg tcttttatat aagaagtatt atacttacca gtctccgtat
     5041 ctaaaagatg ggcccccgcc aaatggcatt ctcccctctt tagtgaaacc aggcctgata
     5101 aactgccaac cgactggata cttagttcaa agtcatattt atttttcttg accagatctc
     5161 tgattagatc aattgtcaag tcgtggctgc ccaccatcat taaattcttc tttattgtat
     5221 ttctcgtttt taataaatag acatctgctt cttctccagc caagatacct tctttgcttt
     5281 cgggaatcgg taatattccg tccgcattga ttaaggactg catggtggca gaacctcttt
     5341 tctgcggtaa ggctaataat tggccatcta tctctgccag gtttaccctg ataaattctt
     5401 tcaaaccggc cgtggaaggg agtttctttt tgacctctgc ctttacctta tccggcagtt
     5461 cataattaag tcccaacatt tttttaacta aaggttcaac aaaaagatga aaatccagta
     5521 aagctgagag aggatagccc ggcaaaccga tcactgcctt ttcatctact tctgctatga
     5581 taagaggttt ccccggcata atatcaacac cgtgaacaaa tacttcaccc aattcctgca
     5641 ggatgcttaa agtataatct tctttaccgg ccgaagaacc ggcattgatg atcgtaatat
     5701 cagaatcagc tacagccttt aatactgccc ttttaatctg ttcataatca tcgggaacta
     5761 tatcgaaatc atgaactgta cagcccagat tttgagttgc cagttttaac atggcggtgt
     5821 taaaatcaac cagctgtcct ttttcttttt tctgagccgg cttgactagt tcactaccgg
     5881 tgggaataat actcactttt aggttttgat atacctctag ctcggttact cccgcttcca
     5941 gcattgcccc tacttcataa acattcactt ctttatgaga gggaacaatc atctgttgtt
     6001 ttactacact ttccccgata gatctaatat tgttccagac cggtacactt ttaatcacct
     6061 ctaccctttt tccatttatc ttctctac
//
LOCUS       DATGCJ010000170         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_67169, whole genome shotgun sequence.
ACCESSION   DATGCJ010000170 DATGCJ010000000
VERSION     DATGCJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67169"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..2476
                     /locus_tag="VKN64_08215"
     CDS             5..2476
                     /locus_tag="VKN64_08215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMA60260.1"
                     /translation="MTDNVTLNIEGMSCAACSQAVENALNKREGVKEANVNLPAEKAY
                     VKYDPLALTVEDLIETVRNSGYDVKRKKEKISFKVAGMNCASCSQAVENTLAAADGIY
                     EANVNIANDTGTVVYDSNIISKEELIELVESAGYELDKFYDEEEYAKSKEEDMEKVEK
                     AKRKMIGTWLFTIPIMLWMIPEMFFGVAWPNHTIFNLGMILLAVPPLVYFGRQTYVSA
                     YKSVIHGGANMDVLIAMGTGAAFITGPFVFFTPMANYAGISAMIMAFHLTGRYIEEKA
                     KGRASQAIKKLLEMEAKTATIIEDDKEKEVPIEEVEPGDIMVVKPGEKIPTDGEIVEG
                     STSIDESMATGESMPVERSVGDEVIGATVNQNGLIKVKATKVGKDTFLSQVIKMVEEA
                     QGTKVPIQEFADKITSIFVPTVITIALSTFILWLVFPGVFRSVGIWAQTFIPWVNPTL
                     GTLTLALFATIAVLVIACPCALGLATPTALMVGSGMGAENGVLIRKGEAIQTMKDIHT
                     IVFDKTGTITKGKPEVTDIITFNGYDQDEMLKTAASAESGSEHPLGQAVVNRAKDNEL
                     KLKEITNFQAVTGKGVEAKLDEKEVLVGSKNLMKEKDIEVTGQVKDKMHGLEDEGKTA
                     MLTAYNRQIIGIIAVADTLKEDSIKAINELKELGIETAMITGDNQRTAQAIAEKVGID
                     HVVSEVLPDGKVDEIKNLQDRFGTIAMVGDGINDAPALTQANVGIAIGTGTDIAIESS
                     DITLVRGDLSSVVTAVKLSRATFRKIKQNLFWAFIYNLIAIPIAVMGLLHPVIAEIAM
                     ASSSVTVVTNANTLRRVNIQPEYKQ"
     gene            2543..2842
                     /locus_tag="VKN64_08220"
     CDS             2543..2842
                     /locus_tag="VKN64_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60261.1"
                     /translation="MILFWILLIVGVVYLISRSHSGLFSKNHNTHRRDYRPGPKEGPK
                     RREYETTDSELFEERDYRNENINRDKSAEEIAKERYAKGEIDKEEYKDILKEINR"
     gene            2972..>3397
                     /locus_tag="VKN64_08225"
     CDS             2972..>3397
                     /locus_tag="VKN64_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMA60262.1"
                     /translation="MEKVVTFTLNPAIDKSSSVEHVIPESKLYCDKPLYEPGGGGINV
                     SRAIKKLGGKSLLMYISGGLSGKRLTNLLQKEELNIQPIHIEKPVRENLIIMENATNL
                     QYRFGMPGPEIKETEIKNIFKTIKNINQFPKYFVISGSIP"
BASE COUNT         1182 a          585 c          763 g          867 t
ORIGIN      
        1 ccaaatgacc gataatgtca cattaaatat tgagggtatg agctgtgctg cctgttcaca
       61 ggccgtagag aacgccctta ataagagaga gggagtcaag gaagcaaacg tgaatttacc
      121 ggctgaaaaa gcctatgtaa agtatgaccc ccttgcatta actgtagaag atttaattga
      181 aactgttcga aacagcggtt atgatgtaaa aagaaaaaaa gagaagatct cttttaaagt
      241 agctggtatg aattgtgcaa gctgttcaca ggctgtagaa aacacactgg ctgccgctga
      301 tggtatatat gaagctaatg taaatatcgc taatgatacc ggtactgttg tatatgattc
      361 taatataatt tcgaaagaag aattaataga gttggtagaa agtgccggat atgaacttga
      421 taaattttat gatgaagaag aatacgcaaa gtcgaaagag gaagacatgg aaaaggttga
      481 aaaggctaaa agaaaaatga tcgggacctg gctctttaca ataccgatca tgctctggat
      541 gattccggag atgttctttg gagtagcctg gcctaaccat accatattta atctgggtat
      601 gatcttactg gccgttcctc ccttagtcta tttcggccga cagacttatg tttctgccta
      661 taaatcagta atccacgggg gagccaacat ggatgtcctg atcgccatgg gtacaggtgc
      721 cgcctttata accggtccct ttgttttctt tactccaatg gccaattatg cagggatatc
      781 agccatgatt atggctttcc atctaaccgg tagatatatt gaagaaaaag caaaaggtag
      841 ggcctctcag gccattaaaa aactgcttga gatggaagca aagacagcta ctatcataga
      901 agatgataaa gaaaaagaag ttccgataga agaagttgaa ccgggcgata tcatggtagt
      961 taaaccaggt gaaaagatac cgaccgatgg tgaaatagtt gaaggttcta cttcaataga
     1021 tgaatcgatg gcaaccggag aatcgatgcc ggttgaaaga tcagttggtg atgaagtaat
     1081 tggtgcaact gtaaaccaga acggtctgat taaagtaaag gcaactaaag ttggtaaaga
     1141 taccttccta tcacaggtca taaagatggt agaggaagct cagggtacca aggtacccat
     1201 acaggagttt gccgataaga taacttctat ctttgtacca acagtaatta caatagcttt
     1261 atcgaccttt atcctctggt tagtattccc gggcgttttc agaagtgtcg gcatctgggc
     1321 tcagaccttt ataccatggg ttaatccgac actcggcaca ttgactttag ccctctttgc
     1381 caccatagcg gtcctggtaa tcgcctgtcc ctgtgccctc ggattagcaa ccccgactgc
     1441 cctgatggtc gggagcggca tgggagctga aaatggtgta ctgattagaa aaggtgaggc
     1501 aattcagacc atgaaggata ttcacactat cgtttttgat aagacaggta cgattacaaa
     1561 aggtaaaccg gaagtaactg atatcataac ctttaatgga tatgatcaag atgaaatgct
     1621 caaaacagct gccagtgctg aaagcggttc tgaacacccg ctcggccagg ccgttgttaa
     1681 cagagcaaaa gataatgaac ttaaattaaa ggaaataact aactttcagg cagtgaccgg
     1741 gaaaggggta gaagcaaagc tagatgaaaa agaagtactg gttggtagta agaacttgat
     1801 gaaagaaaag gatatagagg taacaggcca agttaaagat aaaatgcatg gtttagagga
     1861 tgaaggtaaa acagccatgt tgacagctta taacagacaa ataatcggta taatagctgt
     1921 tgccgatacc ctaaaagaag attcaattaa agcaattaac gagttgaaag aattaggtat
     1981 tgaaacagcc atgatcacag gcgataatca aagaactgcc caggcaatag ctgaaaaggt
     2041 tggcattgac cacgttgtct ctgaagtttt accagacggc aaggttgatg aaataaagaa
     2101 ccttcaagat agattcggta cgattgccat ggtgggtgac ggtataaatg acgctcctgc
     2161 tttaacacag gcaaatgtcg gtatcgcaat tggaaccggt actgatattg caatcgaatc
     2221 ctccgatata acactggtta gaggagatct atcttcagtg gttacggccg taaaactttc
     2281 cagggcaacc tttaggaaga tcaagcagaa cttattctgg gcctttatct ataacttgat
     2341 agcaatcccg attgcagtga tgggactatt acaccccgta attgctgaga ttgcaatggc
     2401 ttccagttca gtaactgttg taaccaatgc aaacacactg cgcagggtaa atattcagcc
     2461 ggaatacaag caataatttc aaaaggaaag gtgataaatt atgatgcatt atggattcgg
     2521 ccacttcgga ggtggaatat atatgatact tttctggatc ttgttaattg ttggtgtagt
     2581 ctatttaatc tcccggtccc actccggtct gttcagtaaa aaccataata ctcatagaag
     2641 agattacaga ccgggaccga aagaaggacc caaaagaaga gaatacgaga ctactgattc
     2701 cgagcttttt gaggaaagag attatagaaa tgaaaatata aatagagata aaagtgcaga
     2761 agagatagcg aaagagagat atgccaaagg agaaatagac aaagaagaat ataaggacat
     2821 cttaaaagaa attaaccgat aaaaaggttc cttctaaatt actaccgccc tgagatgggc
     2881 ggtttttatt attcatttaa ataaaggaaa attacgttaa ataaacaagt ttattaatat
     2941 aagaacgaat tataatttag gaggtacaat aatggagaaa gtagttacct ttactttaaa
     3001 tcccgctata gataaaagtt caagcgtaga acatgtgatt ccggaaagta agttatactg
     3061 tgataagccc ctttacgaac cgggaggggg aggtataaat gtatcaagag cgataaaaaa
     3121 attaggcggt aagtcattat taatgtatat ctcaggcgga ttgagtggta agaggctaac
     3181 aaatttatta caaaaagaag aattaaatat tcagcctatc catattgaaa aaccagtcag
     3241 agaaaatctg attataatgg aaaatgcgac taacctacag tataggtttg gcatgccagg
     3301 acctgagatt aaagaaacag aaatcaaaaa catatttaaa accattaaaa atataaatca
     3361 attccctaaa tattttgtga tcagcggcag tatcccc
//
LOCUS       DATGCJ010000171         5073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_336708, whole genome shotgun sequence.
ACCESSION   DATGCJ010000171 DATGCJ010000000
VERSION     DATGCJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336708"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="floA"
                     /locus_tag="VKN64_08230"
     CDS             complement(<1..710)
                     /gene="floA"
                     /locus_tag="VKN64_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489732.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HMA60263.1"
                     /translation="MTIVSILTIFAVLVFIFLFLYFIPLGLWISAIAAGVKISFFNLI
                     GMRLRRVVPSSIVGPLIKSHKAGLTFTSDQLEAHYLAGGNVDTVVDALIAAERAEIPL
                     KFERAAAIDLAGRDVLEAVKMSVNPKVIKTPLVTAVAMDGIQVKATARVTVRANIDRL
                     VGGAGEETILARVGEGIVTTVGSADTHKKVLENPDSISKTVLNKGLDSGTAFEILSID
                     IADVDVGKNIGAQLQTDQA"
     gene            complement(711..2003)
                     /locus_tag="VKN64_08235"
     CDS             complement(711..2003)
                     /locus_tag="VKN64_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HMA60264.1"
                     /translation="MFKNKILLVIIIILLFSFTLNAQDIVYQIPIKGNIDQAMFSFFE
                     RSFNEAVENNAELIIINIDTYGGFVDPAIRIKDLILESKVETITYVSNRAWSAGALIA
                     LAGDRMVMKEGTSIGAAETRPREEKYISALRKEFKATAEARDKNPDVAAAMVDVDIEI
                     EGVIEKGKLLTLTASEAKNNNIANEVVNSDEGFYSYLGLSPNEITLLTPQAAEQFAAF
                     VTNPYISAILLIIGFSALIFEILVPGFGVGGTVGLISLGLFFSGYIINGIASWGIIVL
                     FLVGLLLILLEVFVVPGFGITGIGGMISIFVSLYFLFPSPQIAINIIASTLIATVVLT
                     YFIGKYFGSSRMWSRISLGISQTKDIGYIASIGDEEITNKTGVTITPLRPAGKVDIDG
                     NRVDVVSEGGFIDKDEKVIVIDVKGSKVLVKPYNEEEN"
     gene            complement(2030..2374)
                     /locus_tag="VKN64_08240"
     CDS             complement(2030..2374)
                     /locus_tag="VKN64_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HMA60265.1"
                     /translation="MADCLFCKIVNKEVDTDFVYEDDKVVVFEDIKPQAPVHLLIVPK
                     THNENLLALEDEDFDIIAHIHKVAVKMAKKYDIAEDGFRLVNNNQEKAGQSIFHIHYH
                     LLGGRDLQWPPG"
     gene            complement(2431..3750)
                     /gene="mtaB"
                     /locus_tag="VKN64_08245"
     CDS             complement(2431..3750)
                     /gene="mtaB"
                     /locus_tag="VKN64_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMA60266.1"
                     /translation="MNSVAFYTLGCKVNHYETEAMMDSFKNQGYQIKDFAEKADIYII
                     NSCTVTNQAASKSRKYARRAKRRNKEAKVVMVGCYPQVDEEEVMDIEEIDYILGTSGK
                     SNIVELIEEKLNIDSQDKHLKDNLNYENFAEFEEMSIKDLSETTRAYIKIQEGCNQFC
                     SYCIIPYARGKMRSRDSENIIKEVKNLIIDDNVKEIVLTGIHLGAYGIENNDRYALVN
                     LIESLAELKGLKRIRLSSIEVTEVNDKLLNLMKNNEKICPHLHLPLQSGSNKILKRMN
                     RPYTKEEFKDRVNKFREKVPELAITTDVIVGFPGEGIKEFRETYDFVKEIGFSRLHVF
                     PFSRRENTPAYEMKPRVRGDIKSKNASRLRLLNKRLMSEFNKQYIGETKKVIIEDNTD
                     YKTGLNTGYTDNYIKVLVEAEQDDIEQLKEVKLTDCYDYEALLGNIL"
     gene            complement(3750..4523)
                     /locus_tag="VKN64_08250"
     CDS             complement(3750..4523)
                     /locus_tag="VKN64_08250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMA60267.1"
                     /translation="MHRFFINEDYIENDVVEISGEDFNHISNSLRLQPGDEIIVSPGN
                     GFDYKVELENFDEQTVIGRIIDKEVNQTEPKVNIDLGQAIPKNRNIELVIQKGTEIGV
                     NQIIPLDTERTIVKLSSSKEKRRIKRWQKISKEAAKQSQRGQIPNIENVLDVNNRQMI
                     NNLFSSYDLILVLYAQDDSFSIKEALKTYKGNNVNKILVLIGPEGGFTPEEISTFNEL
                     GKEHKVATINLGKRILRTETAGLVALSNILYEFGDLGGK"
     gene            complement(4557..>5073)
                     /locus_tag="VKN64_08255"
     CDS             complement(4557..>5073)
                     /locus_tag="VKN64_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HMA60268.1"
                     /translation="CQGTGKVTRRRQLTINIPAGVNDGSKLRMSGEGEAGEKNAPSGD
                     LYIIMNVEPHEIFDRKGDDVYCEVPINFVQATLGDEIKVPTLDGKVKFKIPEGTQPGT
                     TFRLKNKGIPHLNRSGKGDQYIKTKVVIPEKLDQEQKELLMKFAEMSGDEINPEKKGF
                     FDKVKNAFGAL"
BASE COUNT         1526 a         1083 c          656 g         1808 t
ORIGIN      
        1 gcttggtcag tctgtaattg ggctccaata tttttaccaa catcaacatc tgcaatatca
       61 attgaaagaa tttcaaaagc tgtacctgaa tctaaacctt tattaagaac tgtcttagaa
      121 atagaatcgg gattctctaa taccttttta tgagtatcag ctgaaccaac tgtagttact
      181 atcccttctc ctactctagc cagaatagtc tcctcaccgg cacccccaac tagcctgtct
      241 atattagctc ttacagttac ccgggctgta gctttgacct ggataccatc catcgctacc
      301 gcagtaacta atggggtttt aattacttta ggatttacac tcatttttac cgcttctaga
      361 acatctcgcc cggccagatc aattgcagct gccctttcaa acttcaaagg tatttcagct
      421 ctttcagccg ctattaaggc atctacaacc gtgtctacat tacctccagc tagatagtga
      481 gcttccagtt gatcactagt aaaagttaac ccagctttat gagatttaat taaggggcct
      541 acaatagaag atggaactac ccttcttaat ctcatcccaa ttaaattaaa gaaactaatc
      601 ttaacaccgg ctgctatagc agatatccat aatcctaaag gaataaaata taggaataaa
      661 aagataaata ctaagacagc aaatatagtt aaaatagata caattgtcat ttaattttcc
      721 tcctcattat atggctttac taacacttta ctacccttta catctatgac aatgaccttt
      781 tcatccttat caataaatcc accttcactt acaacatcta cccggtttcc atcaatatca
      841 accttaccgg ccggtctcag tggagtaata gttaccccgg tcttattagt aatttcttca
      901 tcaccaatgg aggctatata acctatatcc ttagtctggc taatccccag tgaaattctc
      961 gaccacatgc gagaactacc aaaatacttg ccaataaaat aggttaacac tactgtagct
     1021 attaacgttg aagcaattat attaatagca atctgcgggg aggggaataa gaaatataga
     1081 ctgacaaata tggaaatcat accacctata cccgttatcc cgaagccagg aaccacaaat
     1141 acttctaaca gtattaacaa taaaccaact aaaaataata ctataatacc ccaactggca
     1201 ataccgttaa taatgtaacc actaaaaaac aagcctaatg atattaatcc aactgttcct
     1261 cccactccaa agccgggcac taatatctca aaaataagag cactaaaacc gatgattaaa
     1321 agaatagcac taatataagg gtttgtcaca aaagcagcaa actgttcagc cgcctgaggg
     1381 gtaagtaatg tgatctcatt gggactcaac cctaaataac tataaaatcc ttcatcagaa
     1441 tttacaactt cattggctat attattattt tttgcttcgc tagcagttaa agttaacagt
     1501 ttaccttttt caattacacc ttcgatctct atatccacat caaccatcgc ggctgccaca
     1561 tcagggttct tgtcccgggc ctcagctgta gctttaaatt cttttcttaa agctgaaata
     1621 tatttttctt cccggggacg tgtttcagca gcgccaatac tggtaccttc cttcatcacc
     1681 atcctatcac ctgctaaggc gattaaagcc ccggctgacc aggcacgatt agaaacataa
     1741 gttattgtct caactttaga ttctaaaatt aaatccttaa ttcttatggc aggatcgaca
     1801 aaacccccat aagtatcaat attaatgata ataagttcag cattattttc tacagcctca
     1861 ttaaaacttc tttcaaaaaa agaaaacatt gcctgatcaa tattcccctt gataggtatt
     1921 tgatagacaa tgtcttgtgc atttaaggta aaagaaaata gtaaaattat aatgataact
     1981 aaaagtattt tatttttaaa catgctccca cctctttttt ctctctattt taacctggtg
     2041 gccactgcag atctcttcca cctagaagat ggtaatgaat atggaaaata gattgtccgg
     2101 ctttctcctg attattattg accaatctaa aaccatcctc agcaatatca tatttttttg
     2161 ccatcttaac cgcaactttg tggatatgtg ctataatatc aaaatcttca tcttctaaag
     2221 ctaaaagatt ttcattatgg gttttaggga caattaataa atgtactggt gcctgtggtt
     2281 ttatatcttc aaatacaaca actttatcat cttcataaac aaaatctgta tcaacttctt
     2341 tattaactat cttacaaaat aagcaatcag ccattaccct accccctttc ttattataat
     2401 tattctttac tatccattaa aatcctcttt ttacaaaata tttcctaata atgcttcata
     2461 atcataacaa tctgtcagct ttacctcttt taattgttct atatcatctt gctccgcctc
     2521 tactaagacc ttgatataat tatctgtgta tccagtattt aagcctgttt tataatcagt
     2581 attatcttct attattacct ttttggtttc acctatatac tgtttattaa attcggacat
     2641 cagtctctta ttcaaaagcc gtaagcggga ggcattttta ctttttatat cgcccctaac
     2701 cctcggcttc atctcataag caggtgtgtt ctctcttcta gaaaaaggaa acacatgtaa
     2761 tctagaaaaa cctatttctt taacaaaatc ataagtttcc ctaaattcct ttatcccttc
     2821 tcccggaaaa ccaactatga catctgtggt tatggctaat tcaggcactt tttctctaaa
     2881 tttatttacc ctgtctttaa actcttcttt tgtataaggt ctattcattc tctttagtat
     2941 tttattggaa ccactttgca gtggtaaatg gagatgggga catatctttt cattattttt
     3001 cattaaatta agtaatttat catttacttc agttacttca atagaactta acctaatcct
     3061 tttaagacct ttcagttcgg ctaagctttc tattaaatta actaaagcat atctatcgtt
     3121 attttctata ccataagccc cgagatgtat ccctgtcaaa actatttctt ttacattatc
     3181 atctataatt aagtttttta cttctttaat tatattttcc gagtctctac ttctcatctt
     3241 accccgggca tagggaataa tacagtaact acaaaattga ttacaacctt cctggatctt
     3301 gatataagca cgggtggttt cacttaagtc ttttatactc atctcttcaa actctgcaaa
     3361 gttttcataa ttaagattat cttttaagtg tttatcctga gaatcaatat ttaacttttc
     3421 ttctattaac tcaactatat tacttttacc agaggtccct aatatataat caatttcttc
     3481 aatgtccatt acttcctctt catcaacctg aggataacag cctaccatga ctacttttgc
     3541 ctctttattt cttcttttgg ccctcctggc atattttctg gatttactgg ccgcctggtt
     3601 tgtaactgtg cagctattaa taatgtagat atcggctttt tcagcaaaat cttttatttg
     3661 ataaccctga tttttaaaac tatccatcat agcttcagtt tcataatgat taactttaca
     3721 acccagggta taaaatgcaa cactattcat tattttcctc ctaaatctcc aaactcatat
     3781 aatatgttgg agagggctac caatccggct gtttctgtcc ttaatattct tttacccaaa
     3841 ttaatagttg ctaccttatg ttctttgccc aactcattaa atgtggaaat ttcctcaggg
     3901 gtaaacccac cctcaggacc gattaatact aatattttat ttacattatt tcctttataa
     3961 gtttttaggg cctctttgat actaaacgaa tcatcctgag cataaagtac taatattaaa
     4021 tcatagcttg aaaatagatt atttatcatc tgtctattat taacatctaa aacattttct
     4081 atattgggaa tttgacctct ttgtgattgt ttagcagctt cttttgatat tttctgccat
     4141 ctttttattc ttcttttttc tttactactg gaaagtttta caattgttct ttcagtgtcc
     4201 agaggaataa tttgattaac ccctatttca gtaccttttt ggataactaa ttcaatattt
     4261 ctattttttg gaattgcctg gcctaaatca atattaactt tcggttcagt ttgattaact
     4321 tccttatcaa taattctgcc gatcacagtt tgctcatcaa aattttctaa ttcaacttta
     4381 taatcaaagc cattacccgg acttacaata atttcatcgc ccggctgtaa tcttaaagaa
     4441 ttagaaatgt ggttgaaatc ttcaccagaa atttcaacca catcattttc aatataatct
     4501 tcatttataa aaaatctatg catggtctgt tgttaccttc cttttgaaaa gaatatttat
     4561 aatgcaccaa atgcattttt tactttatcg aaaaaaccct ttttttctgg gttgatttcg
     4621 tcaccactca tttcagcaaa tttcatcaac agttcttttt gttcttgatc taatttttca
     4681 ggtattacta ccttggtttt gatatactgg tctccttttc cacttctatt aaggtgtggt
     4741 atacctttat tttttagtct aaaagtagtt ccgggttgag ttccttctgg gattttaaac
     4801 ttaactttac catctaaagt aggtactttg atttcgtctc ctaaagtagc ctgtacaaag
     4861 ttaattggta cttcacagta tacatcatcg ccttttctat cgaatatttc atggggctca
     4921 acattcatta taatatatag atctccgctg ggagcgtttt tttcacctgc ttcaccttca
     4981 ccggacattc ttagcttact cccatcatta actccggctg gtatgttaat agtaagctgt
     5041 ctacgacggg ttacttttcc agttccctga cag
//
LOCUS       DATGCJ010000172        13529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_23740, whole genome shotgun sequence.
ACCESSION   DATGCJ010000172 DATGCJ010000000
VERSION     DATGCJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23740"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VKN64_08260"
     CDS             <1..838
                     /locus_tag="VKN64_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60269.1"
                     /translation="DFKKLIEKKKEEMKKKPSNQTSKTDIDKHVPKEEAVKKIYDVII
                     NNSHIPPKFDNASFSTFKFNACPKENVKKIKAIKTYAENILTAIKGPQSVYIYSKFNG
                     CGKTHVAIAALKTAAKVFAEEIYKQKPEYYSRRGVSFSDYNWRPVFFMSEVNYLWKKR
                     QFNSSNQDLKRELDTIENAVMNSKLLVIDDFLRERNTDFVFGNLTAWLNHRYDYNKPV
                     IFTSNSDFFKLAQNNESNPYYQTNHLKAATYLASRISEMTKGYQFKFASSPDEDYRQN
                     SY"
     gene            857..1210
                     /locus_tag="VKN64_08265"
     CDS             857..1210
                     /locus_tag="VKN64_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60270.1"
                     /translation="MLSRTETLLKLIELRKMIQAKEQKSADDFTYHAEIKEHIEELIH
                     DIVGLPEEARYVINEIINKVINQNISTKTAVVAIHEIYKEFNDINKIKKENKQKKKKR
                     EQNFGQKKLYKNGYR"
     gene            1338..1943
                     /gene="udk"
                     /locus_tag="VKN64_08270"
     CDS             1338..1943
                     /gene="udk"
                     /locus_tag="VKN64_08270"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HMA60271.1"
                     /translation="MSQLLIGIAGGTASGKTTLAKILKNAFQDEVTMLRHDFYYHDKS
                     HFSVPEDKINFDHPDSFETDLLIDHLKQLIEGAPIARPVYSYKTNERLHETKKVNPSP
                     IIIVEGILIFHYPELRDLFDLKIYIDTDADIRLLRRINRDIKKRDRTFDSVRNQYMAT
                     VKPMHEKYVEPTKYLADIIIPHGGLNDIANDLIIKKIKSHL"
     gene            2022..2387
                     /locus_tag="VKN64_08275"
     CDS             2022..2387
                     /locus_tag="VKN64_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60272.1"
                     /translation="MIKIKTKIVYSVLTLAVVFLLMTVFEVYEARKIRQNYKAVTLEK
                     FKKEKFRHYSQNSEEEERVKIHISGAVKNPGFYEVEPGIKLKRIINSIIILREDADLA
                     KINLNKKIYKKDQIIIPHN"
     gene            2426..4738
                     /locus_tag="VKN64_08280"
     CDS             2426..4738
                     /locus_tag="VKN64_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HMA60273.1"
                     /translation="MKLDNEFLIKAVLFLIVGLITGYYFLDFRLILILSVGMILIFLL
                     LDNTKKLYMIILIIIFLLGFFYLLYYEAGYNSKYSITGYNGQTVEVIGKIKYNLDSLE
                     GRSLRLRPILVDNKRVKHGKIQLYKDQVKPDINHNDLIIARLRLNKPKTKRNPGGFSY
                     YKYLKNQKVYSVANIFEIYKIQSNPGFINIIINIKRVLLNSINNKVSPPVNEFIKALI
                     LGEKSGLNQNWENNFRKAGANHLLAISGLHIGFITIFLLAILTPLPVSQTGKNIILTI
                     FLFIYILITGLRASVLRASFLGLTYKYCKQFNIEVEFPGLISLVLFLVLLVNPYQLFS
                     IGLQLSFLVLIMIVSWTKLLQNYLYTGLAVSIAAQLGSIPLTAYYFNTVTPAGIITNL
                     WAIPLVSGIVFLVLIHFTFYLFIPVLSNFTGKLIFILSKILKDGINIMSDLPSAEIKV
                     TTPSFLTVILFYIIIFLLSYLLKIKDNIKYRKKFKYLSFLLIIITLITVTILVLPVPD
                     SNLLKIYCLDVGQGDSIFIKLPNQKNILVDTGNTIGASSNVKRTIIPFLLNRKIKVID
                     YLLITHFDGDHCSDADYLIKNGFVKNLIISKKFDKDSKKVKDILKSAAEKDIRILTVA
                     NQDILRFEKAKLEFLGPIKDKVYQNSNNKSIVFNLRYKDFEMLFTGDIEREAESDLVD
                     NLSDELLNSDILKVAHHGSITSSLKEFISRVSPKEAVISVGINDFGHPGQKILDRLRK
                     MNSRIWRTDTSGAVIIKTNGNKYSINSYIN"
     gene            4809..5810
                     /gene="holA"
                     /locus_tag="VKN64_08285"
     CDS             4809..5810
                     /gene="holA"
                     /locus_tag="VKN64_08285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMA60274.1"
                     /translation="MSKKVEQIFEKELSQLKNIYLVFSSEEYLLDKFEKRFKDNFIEE
                     SIRDFNLSYIKEEEEVFKKIASQGNTLPIMAEKRFIIVKAGNIFKQKIGNFELLKKFV
                     KDIPKSTILIIIINEDLSKNKRKEIIDEYGEIINLAPPKYQNLDKWIKLQFKQRGKRI
                     NYRGIKILEKMFNNNLQQLDSEIDKICTYIDEKKKVELKDIKKVISRDRRLKENEIFE
                     LLDAISNREKEKSVYLYQHMITSGEVPQIIFAMITRRMKLMLIIKDLKKEGMNYKPIA
                     QKIGIHPYPVKKIYKYVDNYSFKELEALLDKFLQADIRVKTGQDQVEDAILNAIITI"
     gene            complement(5861..6133)
                     /gene="rpsT"
                     /locus_tag="VKN64_08290"
     CDS             complement(5861..6133)
                     /gene="rpsT"
                     /locus_tag="VKN64_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMA60275.1"
                     /translation="MPIIESAKKRVRQNEKKKKVNDRWKNKLKNVIKEFEAAIEEGNV
                     EEAEEMLPDVYKIIDKSASHNIIHKNNAARKKSKMKRMLNKLKNEN"
     gene            6337..8124
                     /gene="lepA"
                     /locus_tag="VKN64_08295"
     CDS             6337..8124
                     /gene="lepA"
                     /locus_tag="VKN64_08295"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMA60276.1"
                     /translation="MDCQHIRNFCIIAHIDHGKSTLADRLLEYTNTIADREMKEQVLD
                     NMDLERERGITIKAQAVRINYNDYELNLIDTPGHVDFSYEVSKSLAAAEGALLVIDAA
                     QGVEAQTMANIYLALENDLEIIPIINKIDLDSANPQMVKEQLLEIGIDPDEAILTSAK
                     EGTNIEEVLNAVIERIPAPEENRDKPLRALVFDSVYDSYQGVIAYVRVVDGKVKVHDH
                     IKMMANDNKYEVDELGVFNPTMTSVDNLEAGEVGYIIAGIKDVRNCRVGDTVTLSNNP
                     APKPLPGYKKVKPMVYSGLYPSDNNDYNLLKDALEKLQLNDASLLFEPETSEALGFGF
                     RCGFLGLLHMEIVQERLEREYDLDLITTAPSVIYKVETEDGEHLEIENPADFPEQNEI
                     EVIKEPYVEATIHVPEEYIGSIMELCQENRGEFIDMQYVDQNRVALQYKMPLSEIITD
                     FFNQLKSKSRGYATLDYDMVGYEESDLVKLDILVNKEPVDALSTIVFEEDAHYEGQKL
                     VRKLKKLIPKQMFKVPVQAAIDNNIVARVNIPAKRKDVLQKCYGGDVSRKRKLLEKQK
                     QGKKRMKKVGKVDIPQEAFMAVLERDEDE"
     gene            8117..9271
                     /gene="hemW"
                     /locus_tag="VKN64_08300"
     CDS             8117..9271
                     /gene="hemW"
                     /locus_tag="VKN64_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMA60277.1"
                     /translation="MSKYLNYDSPFGLYIHIPFCKSKCNYCDFYSIKYNDEKKQQYIK
                     ALSKEIELYTDRLRNETVKTIYFGGGTPSVLSPKKVEHIINKITSNFNLIDDIEISME
                     ANPASLNLEKIIKYKEAGINRLSLGVQSFLNKELKFLGRIHTSKEAVKTIEQVKKYFD
                     NFSLDIIFALPGQKLEDFKYSLNKAINFKPVHLSLYNLQIEEDTPLYNRLEAGEIEPI
                     SEKLDYQMYNFAIKRLKDIGYQHYEISNFAQAGYRSQHNLLYWQYKPYLGLGPSAHGF
                     NGKKRYYNIIDLNSYVDVLSKDKLPIKKKINLSKDELISEMMFMGLRLIEGVSKRDFQ
                     ERFNVSIHDIYGEAIKKLKKDNLLKESDKKLYLTKKGINLGNISFAEFLL"
     gene            9389..10435
                     /gene="hrcA"
                     /locus_tag="VKN64_08305"
     CDS             9389..10435
                     /gene="hrcA"
                     /locus_tag="VKN64_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HMA60278.1"
                     /translation="MVANLDERKKNILHAIIQEHIITASPVGSRTIAKKYDLGVSSAT
                     IRNEMADLEELGYLEQPHTSAGRIPSDKGYRYYVDILMNNKGINSEKLIKTLSEITKD
                     RHGLHEIVSSMTTMLSEFTKYATMITEPEFKDIQISELQLMKVTNNKLLLVMVTDNGF
                     VSDKLINTKEELSRRKIRYINNFLINKIKDKNISQLDKDYFEEIEKELVQKINLSQKL
                     FTIINDEIEQLIKPEDVKIYIGGTSYILDQPEFNDIEYLKKVLNILDHEDMLKKLIKA
                     ENNKELEVRIGHENEMEEMQRCSVVIANYSIGDKAVGKIGVLGPTRMEYPRVISTVNI
                     VSDILSKIISEVSK"
     gene            10435..11016
                     /gene="grpE"
                     /locus_tag="VKN64_08310"
     CDS             10435..11016
                     /gene="grpE"
                     /locus_tag="VKN64_08310"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMA60279.1"
                     /translation="MVANEDNNFENEKGTVQEEKQNMDKKETIDLEEDITEEVMEERL
                     DEIKQQLTEVTEEKDEYLNKLKRLKADFVNYRNRAKKEKQQIEAKTKIEIISSLLPVI
                     DNFERALQSVDEDSEFLSGVKMIHKQLIDVLKKEGLEVIDTEGKEFDPAYHEAVMQVE
                     AEDVDSGFIVEEIQRGYMMEDKVVRPAMVKVAL"
     gene            11061..12932
                     /gene="dnaK"
                     /locus_tag="VKN64_08315"
     CDS             11061..12932
                     /gene="dnaK"
                     /locus_tag="VKN64_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMA60280.1"
                     /translation="MSKILGIDLGTTNSCMAVMEGGEPTVIPNKEGKRTTPSVVAHSK
                     KGEKLVGEYAKRQAITNPDNTIASIKREMGQDLKKKMGDDDYTPEQISAMVLQKMKKD
                     AEDYLGEDIEEAVITVPAYFSDSQRQATKDAGKIAGLTVKRIINEPTAASLAYGLDDE
                     SEQTILVFDLGGGTFDVSILELGDGVFEVISTNGNNKLGGDDFDERIIDYLADEFKSE
                     TGIDLREDKMALQRLKDAAEKAKIELSSVKQTNVNLPFITQTEDGPKHLDVDITRAKF
                     EELVEDLIQKTMGPTDQALEDAGFGPSEIDEVILVGGSTRIPAVQEAVKDKIGKDPHK
                     GINPDEVVAVGAAIQAGVLSGEVDDIVLLDVTPLSLGIETLGGVFTKLIDRNTTIPTS
                     KSKIFSTAQDNQTAVDIHVLQGEREMAKDNKTLGRFQLTDIPPAPRGVPQIEVEFDID
                     ENGIVHVSAKDKGTGNEQNITIKSTSNLSEEEIDEMVKEAEEHAEEDEKKRKEVETKN
                     EADALIHTTKKTLDKAGDKVDDSLIQKVKDARDELQDAIDEDDIDLIEEKMEALNEQL
                     TELSSQLYSQEQGAQGQAGAQQAGAQQQAGAQQQSGASSAEDDTVDVDYEEVDDENQ"
     gene            13007..>13529
                     /locus_tag="VKN64_08320"
     CDS             13007..>13529
                     /locus_tag="VKN64_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMA60281.1"
                     /translation="MPGKKDYYDILGVDKDASQKEIKKAYRKLAKKYHPDMNDGDEQA
                     SEKFKEISEAYEILSDPDKRKRYDQYGHSGINEEDFNFDDFARGGFGGFEDIFDMFFG
                     GGMGGRTRSSRRRRPQRGRDLQYKLNISFEEAAFGAKKKITIPRSETCPKCNGTGAEN
                     ESDIKTCPKCNGQG"
BASE COUNT         5093 a         1929 c         2541 g         3966 t
ORIGIN      
        1 agacttcaag aaattaatcg aaaaaaagaa agaagagatg aaaaagaagc cctcgaatca
       61 gacttcgaag accgatatag ataagcatgt ccctaaagaa gaagcagtta agaagatcta
      121 tgatgtgata ataaataaca gccatatccc acctaagttt gacaatgcaa gcttttcaac
      181 tttcaaattc aatgcttgtc caaaagaaaa tgtcaaaaaa ataaaagcaa taaaaaccta
      241 tgctgagaat attctcaccg caattaaagg acctcagtct gtctatattt atagtaagtt
      301 caacggatgt gggaagacac atgtggctat tgctgccctg aaaacagctg ccaaagtctt
      361 tgcggaagaa atatataaac aaaaaccgga gtattactcc cgtcggggag tatcctttag
      421 cgactataat tggcgtccgg tcttttttat gagtgaagtc aattatttat ggaaaaaaag
      481 gcagtttaat tcttctaatc aggacttaaa aagggaattg gatacaatag aaaacgcagt
      541 tatgaattca aagttattgg taatagatga ttttttacgt gagcgaaata ccgattttgt
      601 tttcggtaat ttaacagcct ggttaaacca tagatatgat tataataaac cggtgatttt
      661 tacttccaat agtgattttt tcaaactggc tcagaataat gaaagtaacc cctattatca
      721 aacaaatcac ttaaaagcgg ctacctattt agcttctcgg ataagtgaaa tgacaaaggg
      781 ttatcagttt aagtttgcta gttctccgga tgaagattat aggcaaaaca gttattaaag
      841 tggaggcgat ctaagattgt tatcaagaac tgaaacttta ctcaagttaa ttgagttaag
      901 aaaaatgata caggcaaaag aacaaaaatc tgctgatgat tttacatatc atgccgaaat
      961 caaagaacat atcgaagaat taatccatga tatagtaggt ctgcccgaag aggcaaggta
     1021 tgttatcaat gagatcatca ataaggtaat aaaccaaaat atttccacta aaacagctgt
     1081 tgttgccatc catgaaatat ataaagagtt taatgatatc aataaaatta aaaaagaaaa
     1141 caaacaaaaa aagaaaaaaa gagaacaaaa ttttggacaa aaaaagttat ataaaaacgg
     1201 ttatagataa gtcacagttt tgactgtggc ttttttagtg gaaatatata aaatttattt
     1261 tgaaagttac ttgacctcgt ctcgataagt tattatacta taaaataaga ttattaatag
     1321 tgaaaggatg aaaatagttg tcacaactac taattggaat agcgggtgga acagcttctg
     1381 gtaaaacaac tctagctaaa atattaaaaa atgcatttca agatgaagtt acaatgttaa
     1441 ggcatgactt ttattaccat gataaaagtc atttttctgt acctgaagat aagattaact
     1501 ttgatcatcc cgactctttt gaaaccgacc tattaataga ccacttaaaa cagttaatag
     1561 aaggagcccc tatcgctaga ccggtatatt cctataaaac aaatgaaagg ttgcatgaaa
     1621 ccaaaaaagt taatccttct cccattataa ttgtagaagg gattttgatc tttcattatc
     1681 ccgagttaag agacctgttt gatttaaaaa tttacattga taccgatgca gacataagac
     1741 tattaagaag aattaaccgc gatatcaaaa aacgtgatag gacttttgat tcagttcgaa
     1801 accagtatat ggctacagta aagccgatgc atgagaaata tgtggagccc acaaaatatt
     1861 tagccgatat tataattccc cacgggggat taaatgatat tgccaatgat ttaatcatta
     1921 aaaaaattaa atcccatcta taaaaattat ccccaaagcg gggatttttt tattacataa
     1981 aaaggaaatt attaacaaat atagtaatat atagtataga ggtgatcaaa attaaaacta
     2041 aaatagtata ttcagtatta actttagcag tagtcttttt attaatgaca gtttttgagg
     2101 tatatgaagc aagaaaaatc agacaaaatt acaaggcggt taccctggaa aagtttaaaa
     2161 aagaaaagtt cagacattat tctcaaaata gtgaagagga agaaagggtg aaaatccata
     2221 taagtggggc cgttaaaaat cccggttttt atgaagttga acctggtata aaattaaaaa
     2281 ggataatcaa tagtatcatt attttaagag aggatgcaga tttagcaaag ataaatctca
     2341 ataaaaagat ttataaaaaa gaccaaataa taataccaca taattgatat taaataatct
     2401 attcaataaa ggatttgata agatagtgaa actagataat gaatttttaa ttaaagcagt
     2461 attattttta atagtgggct taatcactgg ttattacttt ttagacttca ggcttatttt
     2521 aatcttatca gtgggtatga tcttaatatt tttactcttg gataatacca agaaacttta
     2581 tatgataatt ttgataataa ttttcttatt aggtttcttt tacctactat attatgaggc
     2641 aggttataat agtaaatatt ctattacagg ttataacggc cagacggtgg aggttatcgg
     2701 taaaataaaa tataatttgg attcacttga gggcaggtca ctgagattaa gacctatttt
     2761 agtcgataat aaaagggtaa aacatggtaa aatacagctt tataaagatc aggttaagcc
     2821 tgatattaat cataatgatc tgatcattgc ccgccttaga ttaaataaac caaaaacgaa
     2881 aagaaacccc ggtggatttt cttattataa atatcttaaa aatcaaaaag tatatagtgt
     2941 agctaatata tttgaaattt ataaaattca gagtaatccc ggttttataa atataattat
     3001 aaacataaaa agggtattac taaattccat aaacaataag gtttccccac ctgttaatga
     3061 atttatcaaa gcattaatac tgggagaaaa aagcggtctt aatcaaaact gggagaacaa
     3121 ctttagaaaa gcgggtgcta accatctatt ggctatttcc ggcctccata ttggttttat
     3181 tacgattttt ttactagcca tattaactcc attacccgta agccagaccg ggaaaaatat
     3241 aattttaacc atattcctct ttatttatat attaattaca ggattgaggg cttcagtttt
     3301 aagggcttca tttttaggat tgacttataa atactgtaaa cagttcaata ttgaggttga
     3361 atttccgggc ttgatttcac tggtcctctt tttagttttg ttggtcaatc cttaccaact
     3421 tttttccatc ggattacagt tgagcttttt ggttttgatt atgatagtca gctggactaa
     3481 gctacttcag aattatttat ataccggttt agctgttagt attgctgccc aattaggttc
     3541 tataccacta acagcctact attttaatac agttacaccg gccggcataa ttactaatct
     3601 ctgggcgata ccattagtct ccgggatagt atttttagtc ttaattcatt ttactttcta
     3661 tttatttata cctgtattaa gtaactttac tggtaagctt atatttatat tatcaaaaat
     3721 tttaaaagat ggtattaata taatgtcaga cttaccctct gcggaaatta aagttactac
     3781 cccttctttt ttgaccgtta tcttatttta tataattata tttttattat cttatctttt
     3841 aaagattaag gataatatta agtacaggaa aaaatttaaa tacctatctt ttttattaat
     3901 aatcattact ttaataaccg ttactatatt ggttttacct gttcctgata gtaatttatt
     3961 aaaaatttac tgtctggatg tgggacaggg cgattctatc tttattaagt taccaaatca
     4021 aaaaaatatt cttgttgata ccggcaatac tattggagct agcagtaatg tgaaaaggac
     4081 cataattcca tttttattaa atagaaagat aaaagtgatt gattatttac ttattaccca
     4141 ctttgatggt gatcactgct cagatgctga ttatttaata aaaaatggtt ttgttaaaaa
     4201 tctaatcatt tcgaaaaaat ttgataaaga tagtaagaaa gtgaaagata ttttaaaatc
     4261 agctgctgag aaagatataa gaatattaac agtagccaac caagatatct taagatttga
     4321 aaaagccaaa ttagaatttt taggtcctat aaaagataag gtttaccaaa atagtaataa
     4381 caaatcgata gtatttaatt taagatataa ggattttgaa atgttgttta caggtgatat
     4441 cgaaagagaa gcagaatcag atctagtcga taatttatca gatgagctat taaattctga
     4501 tatcttaaag gttgcccacc acggcagtat tacatctagt ttaaaagagt ttatttcgag
     4561 agttagtcca aaagaagctg taattagtgt aggaattaat gatttcggcc atcccggtca
     4621 aaaaatattg gatagattga gaaaaatgaa tagtagaatt tggagaactg atacctcagg
     4681 ggcggtgata attaaaacta atggtaataa atatagtatt aattcctata tcaattgatt
     4741 atattattta aagtatgtta aaattaatta aatgctaaaa ataaaggatt gaaaggtgta
     4801 tttatgatat gagcaagaaa gtagaacaaa tatttgaaaa ggaactttcc caactaaaaa
     4861 atatatattt agtcttcagt tcagaagaat accttttaga taaatttgaa aaaagattta
     4921 aagataattt tattgaggaa agtattagag attttaattt aagttatata aaagaggaag
     4981 aagaggtatt caaaaaaata gccagccagg ggaataccct acctattatg gctgaaaaaa
     5041 ggtttattat cgtcaaggct ggaaatattt tcaaacagaa gataggaaat tttgaattgt
     5101 taaaaaaatt tgttaaagat ataccgaaaa gtacgatttt aatcattata atcaatgagg
     5161 atttgagcaa aaataaaagg aaagaaataa tagatgaata tggtgaaata ataaacctgg
     5221 ctcccccaaa gtatcagaat ttagataaat ggatcaaact ccaatttaaa caacgcggga
     5281 aaaggattaa ttatagggga attaaaatat tagagaagat gtttaataat aatttacagc
     5341 agttagactc ggaaatagat aagatatgta cttatataga tgaaaaaaag aaggttgaat
     5401 taaaagacat aaaaaaagta atctcccggg acagaagatt aaaggaaaat gagatctttg
     5461 aacttttaga tgcgatcagt aacagggaaa aagaaaaaag tgtttatcta tatcaacaca
     5521 tgattacaag tggggaagta cctcaaatta tatttgcgat gataacccgc cgtatgaaat
     5581 taatgcttat tatcaaagat ttaaaaaaag agggtatgaa ttacaaaccc atagcacaaa
     5641 agattggaat acatccttat cctgttaaaa aaatctataa atacgtggat aactattcct
     5701 ttaaggagtt agaagcatta ttagataaat tcttgcaggc agatataaga gttaaaacag
     5761 gtcaggatca ggtcgaagac gcgattttga acgcaataat cactatataa aaaataaacg
     5821 gcttattcat aaacgaataa gcctttttta atgttataat ttaattttca ttttttaatt
     5881 tatttaacat tctcttcatt ttcgatttct ttctggcagc attgtttttg tgaataatgt
     5941 tgtgggaagc agatttatca ataattttat aaacatcagg cagcatttct tcagcttctt
     6001 caacattacc ttcttcaata gctgcttcaa attcttttat aacatttttc agcttattct
     6061 tccagcggtc attaaccttt ttcttctttt cgttttgtct tactcttttc ttagcagatt
     6121 caataatagg cacaactttc acctccttgt gagtgaacgg tatatttata ttttgttcct
     6181 aaatttgcaa catatcaatt ttaacattag atatataaaa atgcaagtct ttccgctaga
     6241 ttatttactc aaaagccatt tttaagcttt cttgattaat ggaattttca atgttataat
     6301 aatcgcatgc attcatataa aagaaaggag ataactatgg actgtcagca tataagaaat
     6361 ttctgtatca tagcacatat agaccacggt aaatcaacac ttgctgaccg tttacttgaa
     6421 tatacaaata caatagcgga ccgcgagatg aaagaacaag tcctggataa tatggactta
     6481 gaacgcgaaa gaggtataac tatcaaggcc caggccgttc gaatcaacta taatgattat
     6541 gaacttaatc taatagatac cccgggccat gtagactttt cctacgaggt ttccaaaagt
     6601 ttggcagccg ccgaaggagc cctgttagtg atcgatgcag cccagggagt agaagcacag
     6661 actatggcca acatatattt agccctggag aacgaccttg aaattatacc gataattaat
     6721 aagattgatt tagatagcgc caacccccag atggtcaaag aacagctttt agaaattggt
     6781 attgaccctg atgaagccat cttaaccagt gctaaagaag ggaccaatat tgaagaggtt
     6841 ttaaatgctg ttatagagcg gattcccgcc ccagaagaga atagggataa accattaagg
     6901 gctttggtat ttgattctgt atatgattct tatcagggcg ttattgccta tgtgagagta
     6961 gtggacggca aggtaaaagt acatgaccat attaaaatga tggcaaatga taataaatat
     7021 gaggttgacg agcttggtgt ttttaatccc actatgactt cagtagataa tttagaagcc
     7081 ggtgaagtag gttatataat agccggtatt aaagatgtaa ggaactgtag agttggtgac
     7141 actgtaactt tatccaataa cccggcccct aagccactgc cgggctacaa gaaggttaaa
     7201 ccgatggtat acagcggttt gtatccttct gataacaatg actataacct actaaaagat
     7261 gctttagaaa agctgcagtt aaatgatgcc tccctattat ttgaaccgga aacttctgaa
     7321 gcactgggat ttggttttag gtgtggattt ttaggtttac tccacatgga gattgtccag
     7381 gagagattag aaagggaata tgacctcgac ttaatcacaa ctgcccccag tgttatatat
     7441 aaagtggaga ctgaagacgg ggaacatcta gagatagaaa atccggccga ttttcctgag
     7501 caaaatgaaa ttgaagtaat caaagagccc tatgtagaag caaccatcca tgtgccagaa
     7561 gaatatatcg gctctataat ggaactctgt caggaaaacc ggggagaatt tatcgatatg
     7621 cagtatgtag atcaaaacag ggttgccttg caatataaga tgcctttaag tgaaataatt
     7681 acagactttt ttaaccagtt gaaatccaaa tcaagaggct atgctacttt agattatgac
     7741 atggttggtt atgaagagtc tgatctcgtc aagttagata tcttagtaaa taaggagcct
     7801 gtcgatgccc tctcaactat tgtatttgaa gaggatgccc attatgaggg gcagaaattg
     7861 gtaagaaagt taaagaaatt gatacccaag cagatgttta aagtacctgt ccaggctgct
     7921 atcgataata atattgtggc cagggttaat attcccgcta aaagaaagga tgtcctgcaa
     7981 aaatgttatg ggggagatgt aagcaggaaa cgcaaactac tggaaaaaca aaagcagggt
     8041 aaaaagagaa tgaaaaaagt aggtaaggta gatataccgc aggaggcctt tatggctgtc
     8101 ttagaaaggg atgaagatga gtaagtattt aaattatgat tctccttttg gactatatat
     8161 tcatataccc ttttgtaaaa gcaagtgtaa ctattgtgat ttttactcta ttaaatacaa
     8221 tgatgagaaa aaacagcagt atattaaggc attaagcaaa gaaattgaac tttacactga
     8281 tagattgcgg aatgaaacgg tcaaaacgat ctattttgga ggcggtactc ccagtgtttt
     8341 atcaccgaag aaggtagaac atataataaa taaaataaca agtaacttta atctaattga
     8401 tgatattgaa atatcgatgg aagccaaccc ggccagtttg aatttagaaa agataataaa
     8461 atacaaggaa gctggtatta atagattgag cttgggggtt caatcttttc ttaataaaga
     8521 gcttaagttt ttaggtagaa tccatacttc aaaagaggct gtaaaaacaa ttgaacaagt
     8581 aaaaaagtac tttgataact ttagtctcga tatcatattt gctctgccgg gccaaaaatt
     8641 agaagatttt aaatatagtc taaataaagc aatcaacttt aaaccggttc atctttcctt
     8701 atacaattta cagattgagg aagatacacc cttatataat aggctggaag ccggtgagat
     8761 agaaccgata tctgaaaaat tagattatca gatgtataac tttgctatca agagattaaa
     8821 agatataggc tatcagcatt atgagatctc caactttgcc caagcgggtt accgttcaca
     8881 gcacaattta ctttactggc agtataagcc ctatttagga ttaggtcctt ctgcccatgg
     8941 gtttaatggt aagaaaaggt actataatat aatagattta aactcctatg tagatgtttt
     9001 aagtaaagac aaattaccca taaaaaagaa gattaaccta agtaaagatg aattaatttc
     9061 agaaatgatg tttatgggtt tgaggttaat agagggagtc agcaaaagag actttcaaga
     9121 gcggtttaat gtttctatcc atgatatata tggagaggca ataaaaaaat taaaaaaaga
     9181 caatttactt aaagaaagtg acaaaaaatt atatttaacc aaaaagggta tcaacctagg
     9241 caatatcagc tttgcagagt ttttactttg acctatattt tgcccaaaat ttaggataaa
     9301 agtatttgac aaaaacatag tgatatgtta ttttattaat agttattagc actctgctta
     9361 gttgagtgct aaaaaagagg tgaagatgat ggttgcaaat ttagatgagc gcaaaaagaa
     9421 tatactacat gccatcattc aagaacacat tataacagct tctccagttg gttcacgtac
     9481 tatcgctaaa aaatatgatc tcggagttag ctcagctaca atcaggaatg agatggcaga
     9541 tttagaggaa ttaggttatc ttgaacaacc ccacacctca gctggccgta ttccttctga
     9601 taagggatat aggtactatg tggatatcct tatgaataat aaggggatta attccgaaaa
     9661 gttgataaaa acactttcag aaataacaaa ggatagacat ggtttacatg aaattgtatc
     9721 gagtatgaca accatgttat ctgaatttac aaagtatgct acaatgatta cagaacctga
     9781 atttaaagat attcagatta gtgaactgca gttaatgaaa gtgactaaca ataaattact
     9841 gctggtaatg gtaactgaca acggttttgt gagtgataaa ttaatcaata ccaaagaaga
     9901 actatcgcgt agaaagatcc gttatataaa caatttccta attaataaga taaaggataa
     9961 aaatatttca caattggata aagattactt tgaagaaatt gaaaaggaat tggttcaaaa
    10021 gataaattta tcacagaaat tgtttaccat tattaatgat gagattgagc aattaatcaa
    10081 accagaagat gtaaaaatat atatcggggg tacatcctat attctcgacc aaccagagtt
    10141 caatgatata gagtatttaa agaaagtatt aaatatactt gaccatgaag atatgttaaa
    10201 gaaattaatt aaagctgaaa acaataaaga attagaagta agaattggtc atgagaatga
    10261 aatggaagaa atgcagcggt gcagtgtagt tatagcaaac tattcaatag gtgataaagc
    10321 agtaggtaaa ataggggtat taggaccgac cagaatggaa tatccccgcg tgatatcaac
    10381 tgtcaatata gtatcagata tcttaagtaa aattatatca gaggtgagta aataatggtg
    10441 gcaaatgagg acaataactt tgaaaatgaa aagggaactg ttcaggaaga aaaacaaaat
    10501 atggataaaa aagaaacgat agatttagaa gaagatataa cagaggaagt aatggaagaa
    10561 cgtttagatg aaataaaaca acaattgaca gaagttacgg aagaaaaaga tgaatactta
    10621 aataaattaa aaagattaaa agctgatttt gtcaactacc gcaacagagc aaagaaagaa
    10681 aaacaacaaa tagaagcaaa aactaagata gaaattataa gttcattatt accggtaatt
    10741 gataattttg agagggcctt gcagtctgtt gatgaagatt cggaatttct atcgggagtt
    10801 aagatgattc ataaacagtt aatagatgtt ctgaaaaaag aaggtctgga agttatagac
    10861 acggaaggta aagagtttga tcctgcttac catgaagctg taatgcaggt ggaagcggaa
    10921 gatgttgatt ctggctttat tgtggaagag attcagcgtg gttacatgat ggaagacaaa
    10981 gttgttagac cagccatggt taaagtggct ctctagtata cttttaaata aattattgta
    11041 tataaaagga ggaatcttta atgagtaaga tactaggaat tgacttagga actactaatt
    11101 cttgtatggc tgttatggaa ggtggcgaac cgactgtaat accaaataaa gaaggtaaga
    11161 gaacaacacc ctctgttgtt gctcactcta aaaaagggga aaagcttgtt ggtgaatatg
    11221 ctaaacgaca ggctataacc aaccctgata ataccattgc ctcaattaag agagaaatgg
    11281 gtcaggatct aaaaaagaaa atgggtgatg atgactatac accagaacaa atttctgcaa
    11341 tggtattgca gaagatgaaa aaagacgcag aggactactt aggtgaagat atcgaagaag
    11401 cagttataac agtacctgcc tacttctctg attcacaaag acaggctacc aaagatgcgg
    11461 gtaagattgc aggtttaacg gttaaaagaa tcattaatga gccgacagca gcctcattag
    11521 catatggact tgatgatgaa tcagaacaaa caatattggt tttcgaccta ggtggaggta
    11581 catttgatgt ctctatatta gaactaggtg atggagtatt tgaagtgatc tcaactaatg
    11641 gtaataataa actaggtgga gatgactttg atgaaagaat aattgattat cttgcagatg
    11701 aatttaaaag tgagaccggt attgatctaa gagaagataa aatggcttta cagcgcttaa
    11761 aagatgcagc tgaaaaagct aagatagagc tatcaagtgt aaaacagacc aatgttaatc
    11821 taccatttat cacacaaact gaagatggac caaaacactt agatgttgat ataacaagag
    11881 ctaaatttga agaattggtt gaagacctaa ttcaaaagac aatgggacca accgatcagg
    11941 ccttagaaga tgctggattt ggcccctctg agattgatga agtaattctg gtaggaggct
    12001 caactagaat tccagccgtc caagaagcag ttaaagataa aataggaaaa gatcctcata
    12061 aaggtattaa ccccgatgaa gttgtagccg ttggtgctgc aattcaggcc ggtgtattat
    12121 cgggtgaagt ggatgatatc gtactattag atgttactcc cttatcctta ggtattgaaa
    12181 cactcggtgg agtatttacc aaattaattg atagaaatac aacaataccg acttctaaaa
    12241 gcaagatatt ctctactgct caggacaatc aaaccgctgt agatattcac gtcttacagg
    12301 gtgaaagaga aatggcgaag gacaataaaa ctctgggcag attccaatta acagatatac
    12361 caccagctcc aagaggagta cctcaaattg aggttgaatt tgatatagat gaaaatggta
    12421 ttgtacacgt ctctgctaaa gataaaggta caggtaatga acaaaacatc acaatcaaat
    12481 ctacctctaa tctttccgaa gaagaaatag atgagatggt caaagaagcc gaagaacatg
    12541 ctgaagaaga tgaaaaaaag agaaaagaag ttgaaaccaa aaatgaagct gatgcattaa
    12601 tccatacaac caagaaaaca ttagataaag caggcgataa agtagatgac tccttaattc
    12661 aaaaagtaaa agatgcccgt gatgaattac aggatgcaat tgatgaagat gatattgact
    12721 taattgaaga aaagatggaa gccttaaatg aacaattaac tgaattaagt tctcaacttt
    12781 attcacaaga acaaggagcc caaggacagg ccggcgccca acaagctgga gcacaacagc
    12841 aggcaggtgc ccaacaacaa agtggtgctt catcggccga agatgacaca gtcgatgtag
    12901 attacgaaga agtagatgat gaaaatcaat aataattatg ttataataca aaaaaagggg
    12961 ttaattcccc tttttttgat tgtattactt tatgaggtga tttgtaatgc caggtaaaaa
    13021 ggattattat gatatcctgg gtgttgataa agatgcatcc caaaaagaga ttaaaaaagc
    13081 atatagaaag ttagcaaaaa aataccaccc cgatatgaat gatggagatg aacaagcgag
    13141 tgaaaagttt aaagaaatat cagaggccta tgaaatatta agtgaccctg ataaaagaaa
    13201 aagatatgat cagtatggtc actccggtat taatgaagaa gactttaact ttgatgactt
    13261 tgctcgcggt ggttttggtg gttttgaaga tatatttgac atgttctttg gaggaggcat
    13321 gggcggtaga acaagaagca gccgccgacg cagaccgcag cgcggtcgtg acctccagta
    13381 taagttaaat atttcttttg aagaagccgc ttttggagct aaaaagaaga ttacgattcc
    13441 cagatctgag acctgtccaa aatgtaatgg tacaggggcc gaaaatgaat ctgatataaa
    13501 aacctgtccg aaatgtaatg gccagggac
//
LOCUS       DATGCJ010000173         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_291502, whole genome shotgun sequence.
ACCESSION   DATGCJ010000173 DATGCJ010000000
VERSION     DATGCJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291502"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1515
                     /locus_tag="VKN64_08325"
     CDS             199..1515
                     /locus_tag="VKN64_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HMA60282.1"
                     /translation="MKPNILYIHSHDTGRYIEPYGYPVETPNLQNLAEEGALFREAFS
                     TAPTCSPSRASLLTGQYPHNNGQLGLVNRGFELDDPSRHIVNTLKKEGYNSALIGMQH
                     IRKDPKTIGYDKILDVDSNYAKDVTPTALDFIGNSIEAPFFLSVGFEETHRPFPEITD
                     KKEIKYSRPPADIPDTYRTRKDMAAFKESAKQLDHGIGKILLKLKEKDLYKNTIIIYT
                     TDHGIAFPKMKCTLSDLGIGVALIIKGPKIFEEGKVIDSLVSHIDIYPTLCEILDINK
                     PNWLQGKSLIPLLNSNKEEIREKIFGEVNYHTAYEPMRTVRTQRWKYIKHFRNRTKPF
                     LSNTDESLTKDVFMEYNWHNSFVPQEELYDTLLDPTESNNLAYESSHKDILEKMREEL
                     DHWMEKTDDPLKRGEVPRPKEAVVNKDDDQKADQVWNYTDKKEGFH"
     gene            1612..2154
                     /locus_tag="VKN64_08330"
     CDS             1612..2154
                     /locus_tag="VKN64_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HMA60283.1"
                     /translation="MDIILIAVALALDASGVSMGVACGTKLNMKNRISLILSFGFFQF
                     LLALSGALLGNFINTRIIRISDIASGVIIFIIGIILFREGFKREEECIYRRLTFWTVA
                     ILGISVSIDALGIGFSIMHKEMFLNMLNKSLIIGIVAAIFTMFSLYIIRYLKKIIIIE
                     KYSDFIAGIILIIFGINMIL"
BASE COUNT          860 a          296 c          405 g          692 t
ORIGIN      
        1 tatcataatt ggaattgtgt tttattattt atacaaaaaa ggatatttac agttaaagcc
       61 agatagaaaa agcgaagaca ctagaagaca tgttttagaa aaggcaaaag aaaagtatga
      121 aaacggtgaa ctaagtgaag aagaatatga aaagataaaa aaagacctta atcaataaaa
      181 acacgggggt atcattttat gaaaccaaat attttatata ttcattccca tgacacaggg
      241 cgttatattg aaccttatgg atatccggtt gaaacaccca atctacaaaa tttagcagaa
      301 gagggtgctt tatttaggga agcctttagc actgccccga cctgttcacc aagtagagcc
      361 agcctcttaa caggccaata tccacataat aatggccaat tgggtctagt aaacagggga
      421 tttgaattag atgatcccag taggcatata gttaacacct taaaaaagga aggttataat
      481 tcagctctaa tagggatgca gcatataagg aaagatccta aaaccatagg gtatgataaa
      541 atattggatg tggatagtaa ttacgctaaa gatgttactc ctaccgcctt agactttatt
      601 ggaaacagta ttgaggcccc tttcttttta agtgttggtt ttgaagaaac tcaccgccct
      661 tttcctgaaa ttacagataa aaaagaaatt aagtatagca gaccacctgc tgacattcca
      721 gatacctata gaacaaggaa agatatggcc gcttttaaag aaagcgccaa acagctagat
      781 cacggtatag gtaaaatact attaaaatta aaagaaaaag atctatataa gaatacaatt
      841 ataatttata ctactgacca tggaatagct tttcctaaaa tgaaatgtac attatcagat
      901 ttgggaattg gggtagcttt aataataaaa ggacctaaaa tttttgaaga aggaaaagta
      961 atagattcac tagtttctca tatcgacatc tatccaactt tatgtgagat attagatata
     1021 aataagccca actggttaca gggcaaatct ttaataccct tattaaacag caacaaagaa
     1081 gaaattagag aaaagatatt tggagaggta aattatcata ctgcttatga accaatgcgg
     1141 acagttagaa cccagaggtg gaagtatatt aaacatttta gaaaccgaac taaacctttt
     1201 ttaagtaata cagatgaaag tttaacaaaa gatgttttta tggaatacaa ttggcataat
     1261 tcctttgtac cacaagaaga actatatgat actttgcttg atccaactga atcaaataat
     1321 ttagcttatg agtcttctca taaagatata ttagaaaaaa tgcgtgagga gcttgatcat
     1381 tggatggaaa aaacagatga tcccttaaag agaggtgaag taccaagacc taaagaagct
     1441 gtcgttaata aagatgatga ccaaaaggca gatcaggtct ggaattatac cgataaaaaa
     1501 gaaggttttc attaaataaa tcgagaagaa ttgctcaaaa acacctttaa tttatagaaa
     1561 atatttgtta agatatacaa agtaatatag taaataatag ggatggttta aatggatatt
     1621 atattaattg ctgtggcttt agcgcttgat gcttcaggtg tctcgatggg agttgcctgt
     1681 ggaacaaaat taaatatgaa aaacagaata tctcttattt tatcctttgg cttttttcaa
     1741 tttttattag ccttaagtgg agccttatta ggtaatttta ttaatacaag gataattaga
     1801 ataagtgata tagcaagtgg agtaattata tttataattg ggattattct ttttcgagaa
     1861 ggatttaaaa gggaagagga atgtatttat cgcagattaa cattttggac cgttgctata
     1921 ttgggcataa gtgtcagcat cgatgccctg gggataggct tttccataat gcataaagag
     1981 atgtttttaa atatgttgaa taaatctttg ataataggta ttgtagctgc catttttact
     2041 atgttttccc tttatatcat tagatacctt aaaaagatta ttataattga aaaatattca
     2101 gattttattg cgggaattat tttaattatt tttggaataa atatgatttt gtaagatttt
     2161 acagcaggat aatctatatg attaaagaat ataaaagaat agaataaaat taaaaattaa
     2221 ttatcaggta ctttcattta cagaaagttt aaa
//
LOCUS       DATGCJ010000174         8173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_603262, whole genome shotgun sequence.
ACCESSION   DATGCJ010000174 DATGCJ010000000
VERSION     DATGCJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603262"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VKN64_08335"
     CDS             <1..1099
                     /locus_tag="VKN64_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMA60284.1"
                     /translation="KDTMDDTKRINQEFINKGFVAKYNFEDIIGESRANRKVKKVAKK
                     LAKTDLTILIEGESGTGKELFANAIHNSSKRKNGPFLAVNFSALPDKLIESELFGYEA
                     GAFTGAKKEGKKGLFEQADGGTIFLDEIGDISIEVQARLLRVLQEKELMRIGGNKINK
                     VDVRVIAATNKNLWNLIEEGKFRKDLYYRLKIGYLKLPPLREHKEDLRELMDYFIEVE
                     STKPIAIKEEVFNELLKYDWYGNIRELKNTLSYMLAVREGKKITLADIPNRDFFKGEI
                     VIEKDRFNQNLNLTDEEKFLLELIDQLKQRNNVVGRRLLAEKAQNTSFPLTKYQIRNR
                     LNKLEEKGLVKKGKGKKGTALTTLGEKYINQ"
     gene            1245..2417
                     /gene="iadA"
                     /locus_tag="VKN64_08340"
     CDS             1245..2417
                     /gene="iadA"
                     /locus_tag="VKN64_08340"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HMA60285.1"
                     /translation="MNIIIKNAEIFDPNFIGKKDIWVVGDKIISIEDNISENFNLNFL
                     NFKTINAEGKYLVPGFIDTHVHFLGGGGEGGFKTRTPEIKLTDITKGGVTTAIGCLGT
                     DSVTRNLRSLLAKSKALEEEGITTYMYTGSYRIPVNTITEGIREDIILIDKIIGTGEV
                     AIEDHRSSQPTYNEFKKVVAETSIGGMLAGKAGIVNIHMGGGKNGLGYLYKIIEEDIL
                     SPKNMLPTHINRSQTLLEQGRDYAKIGGLIDLTTSSSSNQQKTNECAASLKFLLEEGT
                     PIENITFSSDAQGSLPKFDENGKMTGMRIGQVSSLFAAVRSAVKDLDINLETALKVIT
                     ENPARIFNLENKGKIKKDNDADLVILDKESLEIESVIAKGEILFEDKQAVVKGTFQ"
     gene            2433..3365
                     /locus_tag="VKN64_08345"
     CDS             2433..3365
                     /locus_tag="VKN64_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60286.1"
                     /translation="MSNRKKYWANKALRTIITAIGVTLIAFLILHLTPGDPARIMLGP
                     YASEENIEAVRAKYNLDKPLHIQYLAWMSNLLKGDLGTSIRYNRSVNTLIKNRIGPTL
                     ILTLSGLVISVVLGVVMGILAALKRNSWLDYLTTVQAIFWISIPSFWLGILFLYIFGL
                     KLRWVPIAGMVSFVSVILPALSLGLRQEAWFARPMRAEMLEVLNQEYIRAAKAKGLTY
                     YVVVTKHALRNALIPIITMLALRLPWIIGGSVVVEVVFSWGGLGTLLVNSVKARDYPV
                     VQAILLLISIAVVLANLFADVIYTWVDPRIRMEG"
     gene            3368..4291
                     /locus_tag="VKN64_08350"
     CDS             3368..4291
                     /locus_tag="VKN64_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60287.1"
                     /translation="MSEINTNVNEEINVENIKKELSQLKNKKTSQLSLVWRRFKSNKL
                     ALVGFIFVTMIIFVAIFAPVLTPYEPFATNPSNRLMAPSRAHWLGTDAMGRDVLTRLF
                     YGARIALQIGVLIILFEGLIGILMGVISGFFGGIIDSIVMRLVDILRSFPVIILAMAI
                     AGIMGQGLYNVVIALGIVGWTTYARMVRSKILSIKEADYIEAGYSIGESTFSIITRYI
                     IPNAIPTAIVMISIMMPTALIASATLSFLGLGVQPPTPSWGSMVSAGRDYLLQAPWIS
                     TSAGFFIMFTVLGFNFIGDGLRDALDPTTKH"
     gene            4313..5275
                     /locus_tag="VKN64_08355"
     CDS             4313..5275
                     /locus_tag="VKN64_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60288.1"
                     /translation="MSELLDINDLKVYFNTEEGVVKALAGVNFTVEEGTFHGLIGETG
                     CGKSVTGLSILKLLDNNALIEGGEIIYKDDDLLYKTEKEMQRDYRGNEIAMIFQDPGS
                     SLNPVFTIQDQVEESVKMYKKASTQKYREIVIEELKKVKLPDVEGLLKKYPHQLSGGM
                     KQRVMIAMMLACNPNLLIADEPTTALDVSIQAQFLQVLKNLKDRFNMTVLYITHDLAV
                     VSEICDYVSVMYAGRIVENVPVRELFKNPVHPYSYKLVHSVLGVETNLDDLEEIPGSV
                     PRLINPPKGCRFHPRCEEAIEICKKEVPPLKKIGEKHFVACHRR"
     gene            5272..6252
                     /locus_tag="VKN64_08360"
     CDS             5272..6252
                     /locus_tag="VKN64_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60289.1"
                     /translation="MIKLENTLIEVQNLKKYFETPGGSCKAVNGVDLKIKQGETFGLV
                     GESGCGKSTLVRTILKLIEPTSGKIYLNGEDITGYEKEQLRELRKRMSLVFQDPHSSL
                     NPRMTVFDILSRPFKIHKLTNSNEETMIEIVKLLKLMGMQPEHMIRFPHELSGGQKQR
                     IGVARALAVKPEIIFLDEPTSALDVSVQTKILKLLEKTKKLRDLTYFYISHDINLIRV
                     VSDRIGVMYLGKIVEIGKADSIYDNPKHPYTKGLFASVPEPDPDKKIEGEPLTGEVPS
                     PVNPPKGCNFSPRCPYADDKCREVEPELQYLSDGRRVACHKDNLEQYNLL"
     gene            6275..7825
                     /locus_tag="VKN64_08365"
     CDS             6275..7825
                     /locus_tag="VKN64_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60290.1"
                     /translation="MRKSLSVLLVLSLLSGLMIFSAVAFAQDDMSTVDIAIQAEPSKL
                     NPITYQDTETDFVLSQICDPLVEVSPEGSYTAEGAVIEDYMVEEDGKVYVFKIRKGIK
                     FHNGEDLTAEDVKFTYESLMDKDLASPHRSYYEGIEKLELVDEYTLKVTLGETNVTFL
                     TNARLRNHVLPKDYIEEVGWDGYEQKPVGTGPYVFVEHVPGQRIVLKANEDYWDGDPH
                     IENLIFRFYPETTSAVMALQTKEIDFIAELPAEVFQDLKSKEDVGLKFGTYNKFEDHR
                     ICFNKREDCIFSDVRLRKAVAYAINRHELIELTRGEMAVPAVGRVPGFHPASAPNANA
                     YEEDLDKARELMSEAGYPDGFKTKIFAPSGYRERVLEVQQIQQQLARIGIEVEVVTLE
                     WGTYLDVTAEGQAPMFRERWSAGVPSPYSFIENWHSESGWNSIFGTYENDEVDALIDL
                     IKKTTNQEERWQLYRTVQNLAMDEVASYPLYWPVIGEAYNDQLVIPEHVWNVFKRPIY
                     NVDLWHFE"
     gene            7936..>8173
                     /locus_tag="VKN64_08370"
     CDS             7936..>8173
                     /locus_tag="VKN64_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMA60291.1"
                     /translation="MYLDLIKKRIKNSQIKPLGVKAKYAVLVPMLRLEDKLHLIYEIR
                     SEEIGTQPGEVSFPGGRIEEGETAREAAVRETEEE"
BASE COUNT         2724 a         1364 c         1811 g         2274 t
ORIGIN      
        1 aaaagataca atggatgaca ccaaacgtat taatcaggag tttataaata aaggctttgt
       61 agccaagtat aattttgaag atattatagg agaaagcagg gccaacagaa aagtaaaaaa
      121 agtagctaaa aaactggcaa agacagattt aactattcta attgaaggtg aaagcgggac
      181 cggcaaagag ctgtttgcca atgctattca caatagttcg aaaaggaaaa atggaccttt
      241 tttagcagta aacttcagtg ccttaccaga taagttaatt gaaagtgaac tctttggtta
      301 tgaagcgggt gcttttaccg gggctaaaaa ggaaggtaag aaaggactat ttgaacaggc
      361 cgatggcggg acaatttttt tagacgaaat cggtgatatt tccatcgaag tccaggcccg
      421 cttattgcgc gtactgcagg aaaaagaatt aatgcgaatc gggggtaata agataaataa
      481 agttgatgta agagttattg ctgcgaccaa taaaaatcta tggaatttaa ttgaagaggg
      541 taagtttaga aaagatcttt attaccgctt aaaaattggc tatttgaagc tgcccccttt
      601 aagagaacat aaagaagact taagagaatt aatggattac tttattgaag tggaaagtac
      661 taagcccatt gcaataaaag aagaagtatt taatgaactt ttaaagtatg actggtatgg
      721 taatataaga gagttaaaaa acacccttag ttacatgctg gcagtaagag agggtaaaaa
      781 aattacctta gcagatatac ccaatcgtga tttctttaag ggcgagatag ttattgagaa
      841 ggataggttt aaccaaaatt taaacctaac ggatgaagag aagtttttat tagaattgat
      901 cgaccagtta aaacagagaa ataatgtagt tggtcggaga cttttagctg agaaagcgca
      961 aaatacttcc tttcccctta ccaaatatca aattagaaac cggcttaata aattggaaga
     1021 aaaaggttta gttaaaaaag gtaaagggaa aaaaggcacc gctttaacaa ccttaggaga
     1081 gaaatatata aaccaataag accattaagc cttaatgggc caccaataaa acccattaag
     1141 gcttttttta tataaaaaag cccgtcatat taactttgtt aattggcaca caaattgcat
     1201 aatattatat ggtagaaata tatatttgtg aaaggagttt tttaatgaat attataataa
     1261 aaaatgccga aatatttgat cctaatttca tagggaagaa ggatatttgg gtagtaggag
     1321 ataagataat aagtatagag gataatataa gcgaaaattt taacttaaat tttcttaatt
     1381 ttaaaacaat taatgctgaa ggtaaatatt tggtgccggg ctttattgat acccatgtcc
     1441 attttttagg aggaggggga gaaggcggtt ttaagacccg gacacccgaa attaagttga
     1501 ccgatatcac aaagggagga gttactactg cgatcggctg tttgggcaca gatagtgtta
     1561 cccgaaactt acgatcttta ctcgctaaaa gtaaagcttt agaagaagaa ggaattacaa
     1621 cgtatatgta caccggttcc taccggatac cggtcaatac aatcacagaa ggtattagag
     1681 aagatatcat attaatagat aaaataatag gaacgggaga agtggcgata gaagaccacc
     1741 gctcatcaca accgacctat aatgagttta agaaagtggt cgcagagacc agtattgggg
     1801 gtatgttggc cggtaaagca ggtatagtta atattcatat ggggggagga aaaaacggct
     1861 taggctacct atataagatt atagaggagg atatcctctc accaaaaaat atgctgccca
     1921 cccatattaa caggagccaa acattacttg aacagggtag ggattatgct aaaataggtg
     1981 gtctgataga tttaacgacc agttcttcaa gtaatcaaca gaaaacaaat gaatgtgcgg
     2041 caagcttaaa atttttactt gaagaaggta ccccaattga aaatataact tttagttctg
     2101 atgctcaggg tagtttaccc aaatttgacg aaaatggcaa gatgaccgga atgcggatcg
     2161 gacaagtatc ctcacttttt gcggctgtaa ggagtgccgt gaaagatctt gatataaatc
     2221 ttgaaactgc cttaaaagta ataactgaaa atccagcccg gatttttaat ttagaaaata
     2281 agggtaagat caaaaaagat aatgatgcag accttgtcat tttggataaa gagagcttag
     2341 aaattgaatc agtaatagca aaaggcgaaa tattatttga agacaaacag gcagtcgtta
     2401 aaggaacttt tcagtaagga ggaaaaaata taatgtctaa taggaaaaaa tactgggcaa
     2461 ataaagcctt aagaacgatt ataactgcta tcggtgttac cctaattgca tttttaatct
     2521 tacacctaac gcccggagac ccggcccgaa ttatgttagg tccttatgcg agtgaagaaa
     2581 atattgaagc tgttagggct aaatataatt tggacaagcc actccatatc cagtatctgg
     2641 cctggatgtc aaatctactt aaaggagatt tgggtacttc cataagatat aatcggtcgg
     2701 ttaatacttt aataaaaaac aggatcggtc cgaccttaat tttaaccctg tcgggactgg
     2761 tgatatcagt tgttctcggg gtggtgatgg gcattttggc tgcccttaaa cggaacagct
     2821 ggcttgacta tttaacaacg gtgcaggcca tattttggat ttcaattccc tcgttttggt
     2881 tgggtatact attcttatac atattcggtt taaagctgcg ttgggtacca attgccggta
     2941 tggttagttt tgtatcggta atactacctg ctttaagttt gggtttaaga caagaagcct
     3001 ggtttgcccg accgatgcgg gccgaaatgc tggaagtctt aaaccaggaa tatatcagag
     3061 cagccaaggc gaaaggttta acatactatg tggttgtaac taaacacgcc ttaagaaatg
     3121 ccttgattcc catcattaca atgttagctt taagattgcc ctggataata ggtggttcgg
     3181 ttgttgtaga agttgtcttt tcctggggtg gtctgggaac cttacttgtc aatagtgtaa
     3241 aagctagaga ttacccggta gttcaggcga tactcttatt aatctctatt gccgtagtat
     3301 tagctaattt attcgctgat gttatctata cctgggtaga tccccgcatt aggatggagg
     3361 gttagcgatg agtgaaatca atactaatgt taatgaagaa atcaatgtag aaaacataaa
     3421 aaaagaactc agccaattaa aaaacaaaaa gaccagccag ctcagcctgg tatggagaag
     3481 gtttaaaagc aataaactgg ctttagtcgg ttttatcttc gttacaatga tcatctttgt
     3541 agctatcttc gcacctgtct taacacccta tgaacctttt gcgaccaatc ccagcaaccg
     3601 actgatggcc ccttcccgtg cccactggct gggtacagat gccatgggcc gggatgtctt
     3661 aacaagatta ttttacgggg cccgtattgc cttacaaata ggtgtcttga tcatactttt
     3721 tgaaggtttg atcggtattc tgatgggagt tatctccggc tttttcggcg ggattataga
     3781 tagtatcgtg atgaggttgg tcgatatctt aagatccttc cccgtgatta tcctggcgat
     3841 ggcgatcgcc ggtatcatgg gacagggatt atataatgtg gtgattgccc ttggtattgt
     3901 aggatggacg acctatgccc gcatggtgcg cagtaagatc ctatcgatca aagaagcaga
     3961 ctatatcgag gcgggttatt cgatcggaga gtctactttc tccattatca caagatatat
     4021 tataccgaat gcgataccca ccgctatagt tatgatcagt atcatgatgc cgaccgcatt
     4081 aatagcgagt gccaccttaa gctttttggg tttaggagta cagccgccca ccccttcctg
     4141 gggttcgatg gtatcggccg ggcgggacta tctcctgcag gcgccctgga tatcaacttc
     4201 agccggcttt ttcatcatgt tcacggtctt aggttttaac tttatcggtg atggtctaag
     4261 agatgcactg gatcccacta ccaaacatta atcagataaa ggggaaatat atatgagtga
     4321 attacttgat ataaatgatt tgaaagtata tttcaataca gaagagggag tcgtcaaagc
     4381 cttagccgga gttaatttca ccgttgaaga agggaccttc catggactga tcggtgaaac
     4441 cggttgtggt aagagtgtga ccggcctctc tatcttaaag cttttagata ataatgccct
     4501 gatcgaaggg ggagagatta tctataagga tgatgacctt ttatataaaa cagaaaaaga
     4561 gatgcagagg gactaccgcg gtaacgaaat cgcgatgatc tttcaggacc ccggctcttc
     4621 tttaaaccct gtttttacaa tccaggacca ggtagaagaa tcggttaaga tgtacaagaa
     4681 agcttctacc caaaagtaca gggagatcgt tatcgaagag ttaaaaaagg taaaactacc
     4741 tgatgtggaa ggtttattaa aaaagtatcc ccaccagtta agtgggggca tgaaacagcg
     4801 ggtcatgatt gcgatgatgt tggcctgtaa tcctaatctt ttaatcgcgg atgagccgac
     4861 cactgcttta gatgtatcaa tacaggccca gttcttacag gtcttaaaga acctaaaaga
     4921 taggtttaat atgaccgtac tctatattac ccatgacctg gcagtggtaa gtgagatctg
     4981 tgattatgta tctgtcatgt atgcgggccg gatagtagaa aacgtaccgg tacgagaact
     5041 gtttaaaaat cccgttcatc cttacagcta taagttggta cactccgtct taggggtaga
     5101 gaccaacctg gatgatctag aagagatacc gggttcggtt ccgaggttga ttaacccacc
     5161 taaaggttgt cggttccatc ctcgctgtga agaagcgatt gagatctgta aaaaagaagt
     5221 accaccgctt aaaaaaatcg gtgaaaaaca tttcgtagcc tgtcatagaa ggtgataaaa
     5281 ttggaaaata cattaataga agtacaaaac ttaaagaaat attttgaaac ccccggtggt
     5341 tcctgtaagg cagttaatgg agtagattta aagataaaac agggagaaac ctttggtttg
     5401 gtcggggagt ccggctgtgg aaaatcaacc ttagttagaa ccatcctaaa attgatcgaa
     5461 ccgacctccg gaaagatcta tctgaacgga gaagatatta ccggttatga aaaagaacag
     5521 ttaagggagt taagaaagcg gatgtcgctt gtttttcagg atccccattc ttctttaaat
     5581 cccaggatga cggtttttga tattctatca aggcccttta agatccataa gctaaccaat
     5641 agtaatgagg agacaatgat agagatcgta aaacttctta aactgatggg aatgcagccc
     5701 gaacacatga taaggtttcc gcatgagtta agtgggggcc aaaaacaaag gatcggggtg
     5761 gcgagagctt tagccgttaa accggagata atctttttgg atgaaccgac ctcagcttta
     5821 gatgtttcgg ttcagaccaa gatattaaag ttattggaaa agaccaagaa gttaagagac
     5881 ctaacctact tttatatttc acatgatatt aacctgatca gagttgtatc agaccggatc
     5941 ggtgtcatgt atttaggcaa gatagtagag atcgggaagg cggattcgat atatgataac
     6001 cctaaacacc cttataccaa aggcttattt gcgtcagtac cggaaccgga ccccgataaa
     6061 aaaattgaag gtgaaccatt aacgggagag gtaccgagtc ccgttaatcc gcccaaagga
     6121 tgtaactttt cacccaggtg tccttatgct gatgataaat gtagagaagt agaacctgaa
     6181 ctacaatatt tatcagatgg aaggagggtt gcctgccata aagataactt agaacaatac
     6241 aatttattat aaaaataaac aggaggttat taatgtgaga aagagtttat cagttctttt
     6301 agtattatca ttattgtctg gtttgatgat cttttcagct gtagcttttg ctcaggatga
     6361 tatgtcaacc gtggatattg ctattcaggc tgagccatct aagttaaatc caattactta
     6421 tcaggacaca gaaaccgact ttgttttaag tcaaatttgt gaccctttag tagaagtttc
     6481 acccgagggt tcctatactg ctgagggtgc agtaattgaa gattatatgg tagaagaaga
     6541 tggtaaagta tatgtattca agattagaaa aggtatcaaa tttcataatg gtgaagactt
     6601 aactgcagaa gatgttaagt ttacatacga atcattgatg gataaagatt tagcttcacc
     6661 acacagaagt tattatgaag gtattgaaaa gttagaatta gttgatgaat atacacttaa
     6721 agttacttta ggtgaaacta atgttacttt cttaaccaat gcaagacttc gtaatcatgt
     6781 attaccgaaa gactatattg aagaagtagg ctgggacggc tatgaacaaa aaccagtcgg
     6841 aactggtcct tacgtatttg ttgaacatgt gccaggtcaa agaatagtac tcaaggcaaa
     6901 tgaagattac tgggatggag atcctcatat tgagaattta atatttagat tctatcctga
     6961 aactacctca gctgtaatgg ccctgcagac aaaagaaatt gactttattg cagaactacc
     7021 ggctgaagta ttccaggatc taaaaagcaa agaagatgta ggactaaaat ttggcactta
     7081 caataaattt gaagaccaca ggatttgctt taacaaaaga gaagattgta tctttagtga
     7141 tgtccgtcta agaaaagcag ttgcctatgc aattaataga catgaattaa ttgaattaac
     7201 aagaggagaa atggcagtac ctgctgtagg tagagtacct ggcttccacc cggcaagtgc
     7261 tcctaatgct aatgcctatg aagaagatct agataaagca agagaactta tgtctgaagc
     7321 aggttatcca gatggattta aaacaaagat ctttgctcct tctggttaca gagaaagagt
     7381 tctagaagta caacagattc agcagcaatt ggcaagaatc ggaattgagg tagaagttgt
     7441 cacacttgaa tggggaactt atctggatgt aacagccgaa ggtcaagctc ccatgttcag
     7501 agaaagatgg tcagctggag taccttcacc ctatagtttc attgaaaact ggcattccga
     7561 atcaggctgg aactctatct ttggtaccta tgaaaatgat gaagtagatg cattgattga
     7621 tctcatcaaa aagactacca atcaggaaga aagatggcag ttatatagaa cagtacaaaa
     7681 cttagcgatg gatgaagttg cttcttatcc actatattgg cctgtaatcg gggaagcata
     7741 taatgaccaa ttagttattc ccgaacacgt atggaatgta ttcaaaaggc ctatctataa
     7801 tgtagaccta tggcattttg agtaaataca ggtcataatt aaatatatca attaaaaggc
     7861 agccccttac gggttgtctt ttttctttga aatatattat aatataataa atgcaatatc
     7921 tttgaggtga aatttatgta tctcgactta ataaaaaaac gcattaaaaa tagtcaaata
     7981 aaacctttgg gtgtaaaggc caaatatgct gttttggttc ccatgcttag attagaagat
     8041 aaattacatt taatttatga gattaggtca gaagaaattg gtacccaacc aggtgaggtt
     8101 tcatttcctg gcggtagaat agaggaaggt gaaacggccc gcgaggctgc cgtgagagaa
     8161 acggaagaag aac
//
LOCUS       DATGCJ010000175         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_201912, whole genome shotgun sequence.
ACCESSION   DATGCJ010000175 DATGCJ010000000
VERSION     DATGCJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201912"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VKN64_08375"
     CDS             <1..1548
                     /locus_tag="VKN64_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60292.1"
                     /translation="RQRYTLEYQKLKERENNTALSFNNGENYIKDPTVQKEKVSYGIR
                     YQKRFNENTLFKGHSIKTTLNSEQNHYIEEEHQLSLSTKISNRFDTNFIYTKRNNNLN
                     LKEYKYQADVISRLSTKLKLNSTYLYEFDNLDSGKQTSLDNFFRLKFSENSYIYWGCL
                     HNLTNDKKHTQYGFDYMTAKNSKFSYKNKVLDTQFRQESYKYQREDGLQLFKIVKKQL
                     QNDQFVKRSFGLKYPINDKSNISFLKNVYNQKEISTTDIIKYQMQFKKRITAGINYLR
                     YELKQSNEKRSGMRGNLNYDNKDSKGSIQYETVKNKSNLYQKRKFNFSYEKVIKPHIL
                     ILIASQYNIENNETTNIKKIDNEKLFKLKYRPIDKNYKLMYKYLKEIQLSKAAGNSKK
                     ENTTQHSLNFHYQINSLFALNTNIQKYLIKQQNDNNLFQNGIQLNRFGINYKFHKDYT
                     LNIEYRTLYQKLLDNKDSGYLVGLQKSINNNLRLALEYNFTDFNHKISELNLKNKGLS
                     LNINYRW"
     gene            1567..2937
                     /locus_tag="VKN64_08380"
     CDS             1567..2937
                     /locus_tag="VKN64_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60293.1"
                     /translation="MNFKEKIIVISIVLTVGITIVSSPVFGQEYLDLSNFTKINNGYW
                     LLNSDNTRVTQLDKTTTDFYYLSEKEIENRIIKGTFKVDTFTYDSDTFGLVFGYQNIN
                     DSYIFAWDNGGLFGAGQIFYHKKQAFVQPAIPGEILFDGRAANAGWEKGKEYNFKAVY
                     LKNNFKLSIDGKEVININGEFPKGKFGFFCFSQAYVNFYNIEVTSADHLIEPPRPPLE
                     EMEEPIKINKNTTDKTLANFDLSYQTYQIVNSSEYEIKKAYVKIEIDPHLKLIEDSLK
                     ISEDQVNSTFFRGDNILKISNFTLQPSEVLDIGFYVKPDSDFTEAEKYENEIGVYSKN
                     NNSLVSDKIMSVIKYNRNSINYSALLIGRVNIFNKEISEHLPFKIITSDGRVIKVDSL
                     GRYHILFNNFSSLLDTEKVVLEIKLPKRYNKYTIAGAKTKLIEVRPGQLIKQDFNLKL
                     GGQNDG"
     gene            2930..3640
                     /locus_tag="VKN64_08385"
     CDS             2930..3640
                     /locus_tag="VKN64_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMA60294.1"
                     /translation="MVNDFSADDYIKKVKEVFEQNKDEDKAVQMEKYMRNKFEFYGIQ
                     AKERRELSKKLQKIKNRPPHAQLSQVIIKLWSADQRELQYFAMELFERYKNDFRIGDI
                     KLMVYMIENKSWWDTVDFIAKKLVGEYFKEYPQFRDEFINKWLTSNNIWLQRTSLLFQ
                     LAYKKDTDETLLFSNIKRLKEIDEFFIQKAIGWSLREYSRTAPKKVEEFINNNKLSNL
                     SSREGMKVIKKNKEKDNK"
     gene            3734..4495
                     /locus_tag="VKN64_08390"
     CDS             3734..4495
                     /locus_tag="VKN64_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HMA60295.1"
                     /translation="MDINFYNQLNKKMEKSKNVYVVTIVEADGELSELKELKFALTET
                     EVFYSNPDKKADLDQIIEQVGKNIILNMHNPELKKLSIEDKGKIELYIEPVSDNPRLV
                     LFGAGHIANQVSKIASLMDFEITIIDDRAEFLNKERFPEADHIIVEKYTDYLKRYEPE
                     KNDYVVIITRGHQFDYEVLSSVIDTDCKYVGMIGSSKKIREVFDRLREVDNASEELLE
                     IVHTPIGLSIGAETTAEIAVAIMAEIVQVRRQKDE"
     gene            4488..4805
                     /locus_tag="VKN64_08395"
     CDS             4488..4805
                     /locus_tag="VKN64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HMA60296.1"
                     /translation="MNKRLIEDVIKANDKYDSLAVATIIDAKSSAPRDVGTSMIIYPD
                     GDTNGTIGGGNQEKEIIEKAVELLKEGKSGRMSFSLTRKEAAKIGWVCGGDIEIYIEN
                     VNL"
     gene            complement(4884..>5507)
                     /locus_tag="VKN64_08400"
     CDS             complement(4884..>5507)
                     /locus_tag="VKN64_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMA60297.1"
                     /translation="IQIYKNQIGKVGSPYIDYYDYEANLTDFSSPSEYDLVNNGITVA
                     VLNDLKLVNLEKEVSDKTNILIENNSIAQTYNDGISVLNMGGLINTTIKGNDIGLTRN
                     NGISYYDLNNYMREPIGFNQQVTPYYNNIEITNNNIKDALQYGIASYNSYDAKILFED
                     NNIEKAAQGGILYFSHPENEMITLQNNSFGENVKPKILPYILDHIIR"
BASE COUNT         2175 a          698 c          955 g         1679 t
ORIGIN      
        1 agacaaagat atacattaga atatcaaaaa ttaaaagaaa gagaaaataa tactgcgctt
       61 agtttcaaca atggtgagaa ttatattaaa gacccgactg ttcaaaagga aaaggtaagc
      121 tatggtatta gatatcaaaa gagatttaat gaaaacactc tatttaaagg acacagtata
      181 aagactactt taaacagtga acagaatcat tatatagaag aggaacatca gttgagttta
      241 tctaccaaaa taagtaatag gtttgatacg aactttatat ataccaagcg gaacaacaat
      301 ttgaatttaa aagaatataa gtatcaggct gatgttatta gccggctctc aacaaaacta
      361 aaattaaata gcacctacct atatgaattt gataatctag attcgggtaa acaaacgagc
      421 ctagataatt tttttaggct caagttttca gaaaattcat acatatactg gggctgttta
      481 cataatctga caaatgataa aaagcatacc cagtatggct ttgattatat gacagctaaa
      541 aacagtaagt ttagttataa aaacaaggtt ttagacactc aatttagaca agaaagctac
      601 aagtatcaaa gagaagatgg tttgcaatta tttaaaatag tcaagaaaca actacaaaac
      661 gatcaatttg taaaaaggtc cttcggccta aagtatccca taaatgataa aagcaatata
      721 tcatttttaa agaatgtgta taaccaaaag gagatcagta ccaccgatat cattaaatat
      781 cagatgcagt ttaaaaaaag aataactgcc ggaataaatt atctaaggta tgaattgaag
      841 caatcaaatg aaaaaagatc tgggatgagg ggtaatttaa attatgataa taaggatagt
      901 aaaggcagta ttcaatatga aactgtaaaa aacaaatcga atttatatca aaagagaaag
      961 tttaattttt cctatgaaaa agttattaaa ccacatattt taattttaat agcaagtcaa
     1021 tataatattg aaaataatga aacaactaat attaaaaaga tagataatga aaagcttttt
     1081 aaactaaagt accggcccat agataagaat tataaattaa tgtataagta cttaaaagaa
     1141 attcaattaa gtaaagcagc cggcaattca aaaaaggaaa acacaactca acattcttta
     1201 aactttcatt atcaaataaa cagtttgttt gcattaaata caaatataca gaaatacctg
     1261 attaaacaac aaaatgataa taatttattt caaaatggga ttcaattaaa ccgatttggt
     1321 ataaattata aatttcataa agattatact cttaatatag aatatagaac actttatcaa
     1381 aaactattgg ataataaaga cagtggatac ttagtaggat tacaaaaatc aataaataat
     1441 aatctaagat tggctttaga atataacttt accgacttta atcataagat aagtgagtta
     1501 aatttaaaaa ataaaggtct ttccttaaat ataaactaca gatggtagga gaaaggagat
     1561 attaacatga atttcaaaga aaaaataatt gtgatttcaa tagttttgac agtaggtatt
     1621 actattgtca gtagtcctgt ttttggtcaa gagtatttag atttaagtaa ctttactaaa
     1681 atcaataatg gttattggtt attaaattca gataatacca gagtaacaca gttagataaa
     1741 accaccaccg acttttacta tctctctgag aaagaaattg agaatagaat aattaagggg
     1801 acgttcaagg ttgatacttt cacttatgac agtgacactt ttgggttggt atttggatat
     1861 cagaatataa atgattcata tatatttgcc tgggataatg ggggattgtt cggagccggc
     1921 cagatatttt atcacaagaa gcaagctttt gtccagcctg caatcccggg tgaaatcctt
     1981 tttgatgggc gagcagccaa tgcgggctgg gaaaagggca aggagtataa ctttaaagcc
     2041 gtctatttaa aaaacaactt caaattaagt attgatggta aagaagtcat taatataaac
     2101 ggtgaattcc cgaaaggcaa gtttggattt ttttgttttt cacaggccta tgtgaatttt
     2161 tacaatattg aagtaacttc ggcagatcat ttaattgaac ctccgcgtcc accgctggaa
     2221 gagatggaag aacctattaa aataaataaa aacacaaccg ataagaccct tgcaaatttt
     2281 gatctttctt atcaaacata tcaaatagtt aatagttcgg aatatgaaat taagaaggca
     2341 tatgtaaaaa ttgagataga tccgcattta aaattaatag aagatagtct gaaaatatct
     2401 gaagaccaag ttaattcaac cttttttcga ggagataata tattaaaaat aagtaatttt
     2461 accttacaac cttcagaagt acttgatata ggattttatg taaaaccaga ttctgatttt
     2521 actgaagcag aaaaatatga aaatgaaata ggggtttata gtaaaaataa taacagtttg
     2581 gtttcagaca aaataatgtc tgttataaag tataatcgaa attcaataaa ctactcagca
     2641 ctattaattg gacgggtaaa tatttttaac aaagaaataa gtgagcacct ccctttcaaa
     2701 attattactt ctgatgggag agttataaaa gttgatagtt taggccgtta ccacatctta
     2761 ttcaataact tttcttctct attggataca gaaaaggtgg tattggaaat aaaactgcca
     2821 aaaagatata ataagtatac gattgccggt gccaaaacta aattaattga agtaagaccg
     2881 ggccagctaa tcaaacagga ctttaatctt aaattaggag gtcaaaatga tggttaatga
     2941 tttttctgca gatgactata ttaagaaagt aaaagaagta tttgagcaaa ataaagatga
     3001 agataaagca gtacagatgg aaaaatacat gagaaataag tttgagtttt atggtattca
     3061 ggctaaagaa agaagagagt tgagtaaaaa attgcagaaa atcaaaaata gacctcctca
     3121 tgctcaatta agccaggtta ttattaaact atggtcagct gatcagcggg aactacagta
     3181 ctttgctatg gaactatttg aaagatataa aaatgatttt cggattggag atattaaatt
     3241 aatggtgtac atgattgaaa ataaatcatg gtgggatacg gttgatttta ttgccaaaaa
     3301 acttgtgggg gaatacttta aggaataccc gcaatttagg gacgagttta taaacaaatg
     3361 gcttacctcc aataatattt ggttacaaag gacctccctc ttatttcaac tagcttataa
     3421 aaaagataca gatgaaactt tattatttag taatataaaa agattaaaag aaatagatga
     3481 attttttatt caaaaagcaa taggctggtc cttaagagaa tattcaagga cagctcctaa
     3541 aaaagtggaa gaatttatta ataacaacaa gttatccaat ttgagtagta gagaaggcat
     3601 gaaggtgatt aagaaaaata aggaaaaaga taacaaatag gacaaataat atttgtagat
     3661 attttaaaaa ggatttcgat tatcatataa tgaatatgga aaataggata aaataaatta
     3721 cgaggtgaaa agaatggata taaattttta taatcagctt aataagaaaa tggaaaaatc
     3781 caaaaatgtt tatgtagtta caatcgtgga agccgatggc gaattatcag agcttaaaga
     3841 attaaagttt gctttaacag aaacagaagt attctatagt aatccagaca aaaaagcaga
     3901 tttagatcaa ataattgagc aagtaggcaa aaatataata ttaaacatgc ataacccgga
     3961 gttaaaaaag ctttctatag aggataaagg caagatagag ttgtacatag aaccagtttc
     4021 tgataacccc agattggttc tttttggggc cggacatatt gctaaccagg tttcaaaaat
     4081 tgcaagttta atggactttg agattactat tatagatgat agagcagagt ttcttaataa
     4141 agaaaggttt cccgaagctg accatattat agtagaaaag tatactgatt atctaaaaag
     4201 gtatgagcca gaaaaaaatg actatgtagt aataattaca agaggacacc aatttgatta
     4261 tgaagtatta agcagtgtta ttgacacaga ttgtaaatat gtaggtatga tcggtagttc
     4321 aaagaaaata agagaggtat tcgatagatt aagagaagtt gacaatgcta gtgaagaact
     4381 gcttgaaata gttcataccc cgattgggtt atcgattgga gctgaaacca cagcggaaat
     4441 tgctgtagca ataatggctg aaattgttca ggtaaggagg cagaaagatg aataaaagat
     4501 taattgaaga tgttattaag gctaatgata aatatgatag tttggcagtg gctacaatta
     4561 tcgatgcaaa atcatctgct ccccgtgatg taggaaccag tatgatcatc tatccggatg
     4621 gagacactaa tggtactatc gggggaggta accaggaaaa agaaattatt gagaaagcgg
     4681 ttgaattact gaaagaaggt aaatcaggcc ggatgtcatt tagcctgacc agaaaagaag
     4741 cagccaagat cggttgggtc tgtggtggag atattgaaat ttatattgaa aatgtaaatc
     4801 tatagttaat tatatataaa cggataaaca tataaaagag gcctcttatc ttagaggcct
     4861 ctttatgagt ttaaaatatg attttaccta ataatatggt ctaatatata tggtagaatt
     4921 ttgggtttta cattttctcc aaatgaatta ttctggaggg tgatcatttc attttcgggg
     4981 tgactgaaat ataatatacc accctgagct gctttttcta tattgttatc ttcaaaaagt
     5041 attttagcat catatgagtt atagcttgct ataccgtatt ggagagcgtc ttttatatta
     5101 ttatttgtaa tctcaatatt attataataa ggagttactt gttggttgaa acctattggt
     5161 tctctcatat aattattgag atcataataa ctaataccgt tatttctagt taaaccaata
     5221 tcattgccct taatggttgt attaataagc cctcccatat ttaatactga aattccatca
     5281 ttataagttt gtgctatact attattttca attaaaatat ttgttttatc acttacttcc
     5341 ttttctaaat ttacaagttt aagatcattt aatactgcca ctgtaattcc attatttaca
     5401 agatcatatt ctgatggact actaaaatct gttaggtttg cttcatagtc gtaataatca
     5461 atataaggac tgccaacctt accaatttga tttttataga tctgtat
//
LOCUS       DATGCJ010000176         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_202185, whole genome shotgun sequence.
ACCESSION   DATGCJ010000176 DATGCJ010000000
VERSION     DATGCJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202185"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..680
                     /gene="phoU"
                     /locus_tag="VKN64_08405"
     CDS             27..680
                     /gene="phoU"
                     /locus_tag="VKN64_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HMA60298.1"
                     /translation="MEQVYEERRKQLLDDLSNMSEVAEEMLQNAIKALDEKDTELAKD
                     VINRDDVMDSYQVTIEEEVTRLLSLDKPLLSDTQYSIALVKISSDLERIADHATNIAE
                     IVLELKNEEYLQSLVMIPELAGIVKDMLDVVLEAFITKNADLAEAACRQDERVDNIYD
                     QIYNKNLKLVSDKEDLQNINQVFRFTNVAKSLERIGDHATNIGEETIFILTKKRTKY"
     gene            764..2152
                     /gene="hydA"
                     /locus_tag="VKN64_08410"
     CDS             764..2152
                     /gene="hydA"
                     /locus_tag="VKN64_08410"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HMA60299.1"
                     /translation="MSIYIKNGKVATEDSIKPLNILIEDDYIKKVDKSDNFTESEIGE
                     SKIIDAEGCYVLPGMIDAHTHYLLKSRNTVTADDFYSGSIASAFGGVTTFIDFIDDEH
                     ERDRTFKDSLDNRKKEARDAVVDYTFHQSVTHFDEQSPQQLRKIKDEGISSIKIFTTY
                     RREGYLIEEDKLYELFKTLKQMELLPIVHAEDNEIIESNEKEYKKEGKTDIKYHPEIR
                     TGEAEAKAVENIAELIKMLKMPGYIVHLSSKEGLEVLRKEQEKGINLKAETTPHYLLL
                     NREYLKREDGYLYFMTPPLRTKEDNESLWDGLIKDDIDVVATDHCAFDITQKKMGDDA
                     FNTLPGIPGSETLFPLIHHFGSKRGLNLVDIVKKLSTNPAKIFGLYPQKGSLKENTDA
                     DIVIFDPEKSRKLENMFMHSNAGYSPYNHIEVEGYPITTISRGNIVLNQGAYRGHRGH
                     GKYIKADKSNLY"
     gene            2487..>3875
                     /locus_tag="VKN64_08415"
     CDS             2487..>3875
                     /locus_tag="VKN64_08415"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA60300.1"
                     /translation="MKKGYNIPVDELKCTCDPDIFSFESTAELEPFEDGIIGQQRAVD
                     AIETGMRIEHEGYNIYISGVTGSGRSTYAKTIADKKAAKKEVPQDVVYVFNFDNPEKP
                     KAMKLPPGTGIRLESDMENLIKDFKEEIPEVFQGEEYDEARNKIMKQYQERSNEIMEE
                     FNKEIREEGFTLQNTARGTVPVPIDEEGEVIKQDEFQKLDEEKKSELREKSQEIQNDM
                     EKMMRRIRDIKSEAQEKLKEMEKKLSLNVIEPRMESLKEKYDDIEEINQYLDRVKHDI
                     TQHLDKFKEGNGDSQQQSLSQLFQQQQSDESFFVRYKVNLIVDNKELQGAPVIFEHNP
                     TYYNLFGKIEAKSQFGTLTTDFTMIKSGAVHEASGGYLIMKVRDLLTKPFAWETLKRI
                     LINQEIVVENIGEQYRSIPISGLKPEGVHIDVKVILIGNPYIYQLLYHYDEEFKKLFK
                     IRAAFDYEMKRNE"
BASE COUNT         1491 a          560 c          759 g         1065 t
ORIGIN      
        1 tttaaagaag gggctgaagt gtctatatgg aacaggttta tgaggagaga agaaaacaac
       61 tactggacga tctaagtaac atgagtgaag ttgctgaaga gatgctgcaa aacgctatta
      121 aagcacttga tgaaaaagat acagaacttg ccaaagacgt aattaatcga gatgatgtta
      181 tggacagcta tcaggttacg attgaagaag aagttacccg attattatcg ctcgataaac
      241 ccctcttgag tgatactcaa tacagtatcg ctcttgttaa gatttctagt gatttagaaa
      301 gaattgctga tcatgctact aatatagccg agattgtgct cgaattgaaa aacgaagaat
      361 acttacaatc cttagttatg attcctgaat tagccggtat agtaaaagat atgctggatg
      421 ttgtactgga agcatttatt acaaaaaatg ctgatcttgc tgaagctgcc tgtaggcagg
      481 atgaaagagt agataatatt tatgatcaaa tctataataa gaacttgaaa cttgtgagtg
      541 ataaggaaga cttacaaaac ataaaccagg tatttagatt caccaatgta gcgaagtcat
      601 tagaaagaat cggagatcat gctaccaaca tcggagaaga gacaatattt atcttaacta
      661 aaaagagaac aaaatattaa tatatataaa aatattaact ttcttaagag cctatcttca
      721 ctttagatag gcttttcttt ttactttaaa aaggaggttg ccaatgagta tttatataaa
      781 aaacggaaaa gtagcaacag aagattctat taaaccttta aatattctga tcgaagatga
      841 ttatatcaaa aaagtagata aatcagataa ctttacagaa tcggaaatag gtgaaagtaa
      901 aataatagat gcagaaggct gttatgtatt gccgggtatg attgatgccc atactcacta
      961 tctcttaaaa tctcggaata ccgttactgc tgatgatttt tacagtggtt caattgcttc
     1021 tgccttcggg ggagtaacta cctttataga tttcattgat gatgagcatg aaagagatag
     1081 aacttttaag gactccctgg ataacaggaa aaaagaagcc cgtgacgctg tggtggacta
     1141 tacttttcat caatctgtaa ctcactttga tgaacaaagc cctcaacagt taaggaaaat
     1201 aaaagatgaa ggaattagtt ccattaagat ctttacaacc taccgcaggg aaggttactt
     1261 aattgaggaa gataaacttt atgaattgtt taagacccta aaacagatgg aacttttacc
     1321 tattgtacat gctgaagata atgagattat agaaagcaat gaaaaagaat ataaaaaaga
     1381 aggcaaaaca gatataaaat accatcccga gattagaacg ggagaagccg aagctaaagc
     1441 cgtagagaat atagctgaac taattaaaat gctaaagatg cccggctata ttgtgcactt
     1501 atcttcaaaa gaaggattag aggttttaag aaaagaacag gaaaaaggga tcaatttaaa
     1561 ggcagaaacc acccctcatt atcttctttt aaatagggag tatttaaaaa gagaagatgg
     1621 ttatctatac tttatgacac cacctcttag aactaaagaa gataatgaaa gtttatggga
     1681 tggattaata aaagatgata tcgatgttgt agccactgac cattgtgctt ttgatataac
     1741 tcaaaagaaa atgggggatg atgcctttaa caccttacca ggtataccag gttcagaaac
     1801 cctcttccct ttaatccatc atttcggctc aaaaagaggg ctgaatttag tagatattgt
     1861 aaagaaactt tctactaatc ctgctaagat ttttggtctc tatccccaaa aaggaagcct
     1921 taaagagaat acagatgcag atatagttat ctttgatcca gaaaaatcaa gaaaattaga
     1981 aaatatgttc atgcattcta atgccggata tagtccatat aaccatattg aagtagaagg
     2041 ttatcctata acaaccatca gccgcggtaa tattgtttta aatcagggcg cctatagagg
     2101 tcatcggggg cacggtaagt atattaaggc agacaaaagt aatctttatt aatgaacttt
     2161 aatgagatag gaaaaaaggg agaaaaaatt tgaaaaattt attgtgaaac cttgcacaaa
     2221 gtaattaatt atgttatact atctttgaaa tcgaaaaaca aggaaactct taaaacattt
     2281 taaacactat attgttaagg atttaaaatt taatataact tatgacaccc cttccctatc
     2341 tgtttaaaca gatagggttt tctaatttat atagtaaatt aataatcaat ggatgattac
     2401 ctaattgata ataattcatt tctagacttt tatttacctt cgtaactatg gtataattta
     2461 agtaacaatt tcggaggtgg taatcaatga aaaaaggtta caatattccc gtagatgaac
     2521 taaagtgtac atgtgatcct gatatttttt cgtttgaatc cacagctgaa ttagaaccat
     2581 ttgaagatgg aataattgga caacaaagag cagtagatgc aattgaaacc ggcatgagaa
     2641 ttgaacatga aggatataat atatatatct ctggtgtaac tggctccggc cggtccactt
     2701 atgctaaaac catagcagat aagaaagcag ccaaaaaaga ggtaccgcag gatgtagtct
     2761 atgtatttaa ttttgataat ccggaaaaac caaaagcgat gaaattacct ccaggtactg
     2821 gaatcagatt agagagtgat atggaaaacc ttattaagga tttcaaagaa gagataccgg
     2881 aagttttcca gggtgaagag tatgacgaag cccgtaataa gataatgaaa cagtaccagg
     2941 aaagatcaaa tgagattatg gaagaattta ataaagaaat tagagaagaa ggatttactc
     3001 ttcaaaacac agctagaggt actgtcccgg tacctataga tgaagagggc gaagtaatta
     3061 aacaggatga atttcaaaaa ctggatgaag aaaagaaaag tgaacttcgt gaaaaaagcc
     3121 aggaaattca aaatgatatg gaaaaaatga tgagaagaat cagggacatt aagtcagaag
     3181 ctcaagaaaa attaaaagaa atggaaaaga aattaagtct aaatgtgata gaaccgagga
     3241 tggaaagttt aaaagaaaag tatgatgata ttgaagaaat taatcagtat ctagatagag
     3301 taaaacatga tataacccaa catctagata aattcaaaga aggtaacggg gatagtcagc
     3361 agcagagcct ttctcagcta ttccagcagc agcaaagtga cgaatcattc tttgttagat
     3421 ataaagtgaa cttgatagta gacaataaag aactgcaagg tgcacccgta atctttgaac
     3481 acaacccgac ctattataat ctgtttggga agatagaagc taaaagtcag tttggtactt
     3541 taaccactga ctttacgatg ataaaaagtg gtgctgttca tgaagcaagc ggcggctacc
     3601 tgattatgaa ggtaagagat cttttaacaa aaccttttgc ctgggaaacc ctaaaaagga
     3661 ttctaatcaa tcaggaaata gttgttgaaa atattggtga acagtatagg agtataccga
     3721 tcagtggttt aaaacccgaa ggtgttcaca tagatgtaaa ggtaatatta attggtaacc
     3781 cctatatcta tcaattgctt taccattatg acgaagaatt taaaaagtta tttaaaataa
     3841 gggcagcctt tgactacgaa atgaagcgga atgag
//
LOCUS       DATGCJ010000177         7571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_383315, whole genome shotgun sequence.
ACCESSION   DATGCJ010000177 DATGCJ010000000
VERSION     DATGCJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383315"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..695
                     /locus_tag="VKN64_08420"
     CDS             162..695
                     /locus_tag="VKN64_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60301.1"
                     /translation="MNKKIILKTKHLNIRKFTIRDTEQIYNLSIEEGIKRWLPDQVYK
                     SKAEAKATLQFLISQYDSVLEPDKNPYILGMELKEKKRLIGHVGLSPFQGEVEIGYAV
                     GNKYQGFGYATEVVSSFSQWVLNNLNLDFIWGIVNKDNKASIKVLEKANYKYIDKENN
                     KFRYKFLRKNDLKSKVK"
     gene            695..1477
                     /locus_tag="VKN64_08425"
     CDS             695..1477
                     /locus_tag="VKN64_08425"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMA60302.1"
                     /translation="MVNKKSNENRIALVTGASRLIGIGAAVCKKLAEEGIDVFFTYWS
                     RYDAKQKWGIEESEPAILKSQIEKHDVRCVKREIDLSNPDNIKVLLDEVVEKLGTPSI
                     LINNACHSTTSNYQDIDSKLMDNHYNINLRLTTLLSSHFARIFDKKEGGRIINLSSGQ
                     FKGPMKNELAYAATKGGVDALTITLAAEVASQGITVNTVNPGPTDTGWMDQTTKEELK
                     EKFPFKRLGRPKDAANLISFLVSKEAAWITGEVINSDGGFYN"
     gene            1522..2349
                     /locus_tag="VKN64_08430"
     CDS             1522..2349
                     /locus_tag="VKN64_08430"
                     /inference="COORDINATES: protein motif:HMM:NF005250.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60303.1"
                     /translation="MIEGLPSTGKSTTAELTKEILDKMNIDNELVLEGNLDHPADYDG
                     AAYFTEGQLKKLKKRFNEYEEFIDKITEQREEGYFISYIKRIKEYEDDFPKELFREIK
                     KYDIYEIPLDLNRKLILNKWERFMKKVQKQDKVYIFECVFIQNPVTVSIIRNNASIDT
                     AYSYIKELKDIIDELNPVLIYLEQNDIERSFNKVIEERPEWWLDFFIDYYTNYEFGEE
                     RNLEGLKGTIKGLKEIDSIQKELISKLEMQKYTLDNSHYNLEKSKNNIKNILKGIIK"
     gene            2442..3056
                     /locus_tag="VKN64_08435"
     CDS             2442..3056
                     /locus_tag="VKN64_08435"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HMA60304.1"
                     /translation="MEQALIIWGGWQGHKPKLTVNILKEELSKNEYKVKLTSNLAVLI
                     NEDLTKYDVIIPIWSVGIKSTVYLKALLDAVREGVGLATFHGGIKWFIDDNYYHLIGG
                     NYIKDSEEESFTVEIVNQNHPVTEYLEDFEITSELYYLQFDPRNEILAEAEFKGVKMP
                     ISWVKNFGDGRVYYTSLAHAPEHLFEKHSLRMLLAGINWAAGRI"
     gene            3127..3960
                     /locus_tag="VKN64_08440"
     CDS             3127..3960
                     /locus_tag="VKN64_08440"
                     /inference="COORDINATES: protein motif:HMM:NF015586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60305.1"
                     /translation="MILGIDIGGSTTDIVGLDNNEIFDLFTVEASDPTTSAYGALGKM
                     LTKNKLNIEDIDKIAITGVGSSYIDSTIFGINTIKIDEFEAIGLGGSFLSGINKTLVV
                     SLGTGTAMVNVDGNKIKHIGGTGIGGGTLLGLGKGILNIIDFDEIVELSKHGNLHKVD
                     LTIGDISNEKVGSLHQSVTASNFGNIHKIANEEDYAKGILNLIYQTIGMLAVFGAQMS
                     GNEDIVFTGKLAQIEEGIAILKALRDNKSYDLNLHFPEHATFSTAIGSAISVLKRQET
                     N"
     gene            3977..4831
                     /locus_tag="VKN64_08445"
     CDS             3977..4831
                     /locus_tag="VKN64_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HMA60306.1"
                     /translation="MKIVYWAVGLVLVLLVLVYLISVFGESSFNQNVADDIDKLIADV
                     NIEQVDKVTEDDLEGLPVPVKRWLEFSQVVGKEKIKTVRLKQVGSMRTKPGQKGMDTK
                     AEQYFNTVDPSFVWKARVNMMPMLYFAGEDRYYDGQGKMLIKVLSLFPVVDAEGKEMN
                     QGTLTRYLGEIIWFPTAALSEYIEWEEIDANTARATMSYKGTSGSAVFNFEDSGKVVS
                     FECKRYYNSDEGYRLEDYYAPVWNYQEFSGIKIPTQAKAIWRLESGDYEYYDMEITEI
                     DYNIKDKY"
     gene            4914..5702
                     /locus_tag="VKN64_08450"
     CDS             4914..5702
                     /locus_tag="VKN64_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMA60307.1"
                     /translation="MEKSKKKRELLLFVIIAIGWSWIFWLAEMLLNIKLYVAPFGPLI
                     AAFYLTYIKENKKGLIKLIKRGLDYKFKKVWYLPIIFLMPIIAGLTIFITSRLGAGSA
                     NVDILTKPLPIIGNFVYILFLGGPLQEEFGWRGYALPRLLKRYNALVASIILGLIWSI
                     WHLPLNFISQAGLQYQAAVSYVISSMILMVFVSILFTWIYTNTGGSILAVLIFHTMLN
                     LSTYVIFPVFELEMGPPIYMFLILIVTLIVIFTFGYKNLTYKKI"
     gene            5739..>7571
                     /locus_tag="VKN64_08455"
     CDS             5739..>7571
                     /locus_tag="VKN64_08455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMA60308.1"
                     /translation="MKRRRIWVFLLLFILLTLSFFNTSNVKAQDLNNIESLRAFLEGF
                     IHSQMEEYNVPGVTLSIVKDGAVLLEEGYGYYDLENESRVNPDTTLFRPGSGTKLFVW
                     TSVMQLVEAGEIDLDADINNYLNFTIPAEVVGHTKENVQPITMLDLMNHTAGFEETLE
                     QLFVLSQENVKPLGTYLKDHVPARVFPAGEQIAYSNYGSALAAYIVERVSGLPFFEYV
                     EQNIFNPLNMERSTLIQPVPENIEGEVSYGYAKINGVFKKDEFEYIQPYPAGSLSTTA
                     SDMSKFMLAHLNNGFFGGNRILEADTVREMHSQSFTHYEEFSGMAHGFIEMNYNGYRI
                     ITHGGDTFLFHSGIYLIPELDLGFYVSYNGRDAALARDSLIKNFLDRYYPSDRDIVPE
                     EAGSNFMDNSKITGTYYPNRSNFTDYQSVVRIITQTNIRLTQAGNLTYNFMGENHQLE
                     QIAPRVFYDKDIGNKLYAPEGEEINRLYTNGPNGLLKAKWYETLNFNLIFLIGYALFG
                     IVFSIVLIKSLFKRYIRNKYILEKITVVIFTVAAIGFFIAIGIFLANTHPLYNVPYLF
                     LEVTPAFDNILLFLWIIPVLAVILVVMNVRIWFKGRWNVIQKGIY"
BASE COUNT         2633 a         1031 c         1574 g         2333 t
ORIGIN      
        1 aaacagctgg tcaatttgga tgcagtggga aaaagcttta aaggagtccg ggttccaaaa
       61 aagagatggt gatattgaat tcctagaagc tgataacggt aagtatgtga gttttcccag
      121 gattgtagcg agaaaaaaat aaatattgat tggtggttaa tttgaataaa aaaataattt
      181 taaaaacaaa acatttaaat attagaaaat ttacaataag agatactgag cagatttata
      241 acttgagtat agaagaagga atcaaaaggt ggctgccgga ccaggtatat aaatcgaaag
      301 ccgaggccaa agccactctt cagtttttaa tttctcagta tgattcagtg cttgaaccag
      361 ataaaaatcc ttatatctta ggaatggaat taaaagagaa aaaaaggtta attggccatg
      421 ttggactcag tcctttccaa ggggaagttg aaattggtta tgctgtcggg aataaatatc
      481 aggggtttgg ttatgcaact gaagttgttt ctagtttcag ccaatgggtt ttaaataatt
      541 taaacttaga ttttatctgg ggaatagtga ataaagacaa taaagcttca attaaggttt
      601 tagaaaaggc gaattataag tatattgata aagagaataa taaatttagg tataaatttt
      661 tgagaaaaaa tgatttaaaa agcaaggtga aataatggta aataaaaaaa gtaatgagaa
      721 tagaattgct ttggtaaccg gggctagccg gttaataggt ataggtgcag ctgtttgtaa
      781 aaaactagcg gaagagggca ttgatgtttt ctttacctat tggtcaagat atgatgcgaa
      841 acaaaaatgg ggtattgagg aaagtgaacc tgccatctta aaaagccaga tagaaaaaca
      901 tgatgttaga tgtgttaaga gagaaataga tctttccaat ccagataata ttaaagtact
      961 attagatgag gttgttgaaa aattgggtac tccctctatt ttgataaata acgcctgcca
     1021 ctctaccact tcaaattatc aggatatcga tagtaaacta atggataacc attataatat
     1081 aaatctccgt ttaacaacat tattaagttc ccactttgcc cgtatctttg ataaaaaaga
     1141 aggtggtcgg ataattaatc tttcttcagg acagtttaaa ggtcccatga aaaatgaatt
     1201 agcttatgct gctaccaagg gaggggtgga cgccctgact ataacactgg cagccgaagt
     1261 agcttcacag ggtattactg ttaatacggt aaacccgggg cctacagata caggctggat
     1321 ggatcagaca acaaaagaag aacttaaaga aaagttccct ttcaaaagac tcgggcggcc
     1381 gaaagatgcc gctaatttaa ttagttttct cgtttccaaa gaagcggcct ggataacagg
     1441 agaagtgatt aattctgatg gtggctttta taattaaaaa ttttagaaaa gttggtgaaa
     1501 aaattggaga gcaaactagt attgattgag ggcttaccga gcaccggtaa atctaccaca
     1561 gctgagctga ccaaagaaat tttggataag atgaatatag ataatgaatt agttttagaa
     1621 ggtaatttag accacccagc tgattatgat ggggcagcct atttcactga aggtcaatta
     1681 aagaaactta aaaaaagatt taacgaatac gaagaattta tagacaaaat tactgagcag
     1741 agagaagaag gatacttcat ttcttatata aaaaggatca aagaatatga agatgacttt
     1801 cccaaagaat tatttagaga aatcaaaaaa tatgatatct atgaaatacc tttagattta
     1861 aatagaaagt tgatcttaaa caaatgggaa aggtttatga aaaaagttca aaagcaagac
     1921 aaagtatata tctttgaatg tgtgtttatc caaaatcctg taacggtcag tataataagg
     1981 aacaatgcct ctatagatac agcctattcc tatattaaag agctgaagga tataatagac
     2041 gaacttaatc ccgtgctcat ttatcttgag caaaatgata ttgagagaag ttttaataag
     2101 gtaattgaag aaagacctga atggtggctg gatttcttta tagattatta tacaaactat
     2161 gaatttggag aggaaagaaa tctagagggt ttaaaaggaa cgataaaggg attaaaagaa
     2221 atcgacagta ttcaaaaaga attaatttct aaattagaga tgcaaaagta tacattggat
     2281 aattctcact ataatttaga aaaaagtaaa aacaatataa aaaatatatt aaaaggaatt
     2341 ataaaataat ttataaaaga ggtctttatc tggatttaaa gaatttaaaa agtagtaaat
     2401 attagtatat ataaccatat tttattaaag aaggtgttgg tatggaacag gccttaatta
     2461 tatggggagg ttggcaaggc cacaaaccca aattaacggt gaatatatta aaagaggaac
     2521 tcagcaaaaa tgaatataaa gtaaaactaa caagtaattt agcagtattg ataaatgaag
     2581 atttgactaa atatgatgtg ataatcccga tctggtcggt gggcataaaa agtacagtct
     2641 atttaaaagc actgcttgat gctgtgagag agggtgtggg tctagccacg tttcatgggg
     2701 ggattaagtg gtttatcgat gacaactact accacctcat aggtggcaac tatattaaag
     2761 acagtgaaga agagagcttt acagtggaga tagtaaatca gaaccatcct gtaacagagt
     2821 atcttgaaga ttttgaaata acaagtgaac tatattacct tcagttcgat ccgcgcaatg
     2881 agattttagc tgaagcagag tttaaaggag ttaagatgcc gataagctgg gttaaaaatt
     2941 tcggtgatgg gcgcgtttat tatacatcat tagctcacgc cccggaacac ttatttgaaa
     3001 agcatagtct aaggatgctt ttggcaggaa ttaattgggc tgctggtagg atataatagt
     3061 aatgtgctat aaggtttatt taaacaaaaa ctcaaaatat aattttttaa ttcagaggtg
     3121 tttttaatga ttttaggtat agatattggg ggttctacta cagatatagt aggtcttgat
     3181 aataatgaaa tttttgattt atttacggtt gaggcaagtg atcccactac atcagcatat
     3241 ggagcattag gtaagatgct gacaaaaaac aaattaaata tagaagatat tgataaaata
     3301 gctattactg gtgttggttc atcctatata gattctacca tatttggaat caataccatt
     3361 aaaatagatg aatttgaagc aatcggcttg ggaggttctt ttttatcagg tattaataaa
     3421 actctggtag taagcttggg gaccggcaca gctatggtaa atgttgacgg taataagatt
     3481 aaacatattg gcgggacagg tatcggtggt ggaacattac tgggcctcgg gaaaggaatt
     3541 ttaaatatta ttgattttga tgaaattgta gaactttcca aacatggaaa cctacacaag
     3601 gtagatttaa ctatcggaga tatctcaaat gaaaaggtgg gttctttaca ccagtctgtg
     3661 accgcttcca attttggaaa tatccacaag atagctaatg aagaagacta tgccaagggt
     3721 atattaaatc tcatatatca gaccattggt atgttggccg tctttggagc ccagatgtct
     3781 ggaaatgaag atattgtatt taccggtaag ttggcacaga tcgaggaagg gattgcgatt
     3841 ttaaaggctt taagggataa caaatcatat gatcttaacc tccattttcc ggaacatgct
     3901 accttctcta ctgcaatagg ttcagctatt tcagtattaa aaagacagga gactaactaa
     3961 actttgaggt gaaaaaatga agattgtgta ttgggctgta ggacttgtat tagtattatt
     4021 ggttttggtg tacttaatat cggtatttgg agaaagttct tttaatcaaa atgtggctga
     4081 tgatattgat aagctgattg ctgatgtcaa tattgaacaa gttgacaagg taactgaaga
     4141 tgatctggag ggattaccag tacctgtaaa aagatggctt gagtttagtc aagtagttgg
     4201 gaaagagaag ataaagacag tccgtttaaa acaggtgggc tcgatgcgga caaaaccggg
     4261 ccaaaaaggt atggatacaa aggctgaaca gtattttaat acagttgacc cttcttttgt
     4321 ctggaaggcg cgagtaaata tgatgcccat gttgtatttt gcaggtgaag atagatacta
     4381 tgatggacag ggtaaaatgc ttattaaggt tttatcactt tttccggttg tagatgcgga
     4441 aggtaaagag atgaatcagg ggactttaac cagatatttg ggtgaaatca tctggtttcc
     4501 taccgctgct ttaagtgaat acatagaatg ggaagagata gatgctaata ctgctcgggc
     4561 cacaatgagt tataaaggga cgagtggttc agccgttttt aatttcgaag attcaggtaa
     4621 agtagtcagt tttgaatgta agcgttatta taatagtgat gaaggttata gattggaaga
     4681 ctattatgct ccggtctgga attatcaaga attttcaggt ataaaaatac ctacccaagc
     4741 gaaagctatc tggagattgg agtcgggtga ttatgaatac tatgatatgg aaataaccga
     4801 gatagattat aatataaaag ataagtatta ataaaaaata ggtgatctag atagatttat
     4861 tttatttgat aaaacccgga tgaattcaaa taaataaatt aaaggagttc aaaatggaaa
     4921 aatccaaaaa gaagagagaa ctgcttttgt ttgtcattat tgcaatagga tggtcttgga
     4981 ttttttggtt ggctgagatg ttgttgaata tcaaattata tgtagcccct tttggacctt
     5041 taattgccgc attttatctg acctacataa aagaaaataa aaaaggtctt attaaactaa
     5101 taaaaagagg tctggattat aaatttaaaa aagtttggta tttacctata atatttttga
     5161 tgccgataat cgcgggtctc actattttta ttacgagtag actgggagca ggttcagcga
     5221 atgtagatat cttaacaaaa ccgctaccta tcattgggaa ttttgtttat atactttttc
     5281 tgggcggccc gctgcaggaa gaattcgggt ggagaggtta tgccttgccc cgacttctaa
     5341 agagatataa tgctttagtg gccagtatca tattaggttt gatctggagt atctggcatt
     5401 tgccgctcaa tttcatttca caagcaggcc ttcaatatca ggcagcagta tcttatgtga
     5461 taagttcaat gattttaatg gtctttgtct cgatcttgtt cacctggatc tatactaata
     5521 cgggtggtag tatcctggct gtcttaatat ttcatactat gttgaatttg tcaacatatg
     5581 taattttccc tgtttttgaa ttggagatgg gaccacctat ctatatgttc ttaatattaa
     5641 ttgttacttt aattgttatt ttcactttcg gttataaaaa tttaacttat aaaaagatat
     5701 aatcatggaa aaattagaaa actaaggaga tggcagcgtt gaaaagaaga agaatttggg
     5761 tctttttatt actttttatt ttattaacat tatctttctt taatacttca aatgtaaaag
     5821 ctcaggatct aaataatata gagtctttaa gagctttttt agaaggtttt attcattctc
     5881 agatggaaga atataatgtg cccggggtaa ctctatctat agttaaagat ggggcagttc
     5941 tcttggaaga aggatatgga tattatgatc tagagaatga aagtagagtc aatccagata
     6001 cgacattatt tagaccgggc tctggtacca aactatttgt ttggacctct gtcatgcagc
     6061 tagtagaagc cggtgaaata gatttagatg cagatataaa taactattta aactttacaa
     6121 taccagcgga agtggttgga cataccaaag aaaatgtaca accgataaca atgctggatt
     6181 taatgaacca tactgccggt tttgaagaaa cccttgaaca gttatttgta ttatcccagg
     6241 agaatgttaa gcctttgggt acctatctaa aagaccatgt gccagcccga gtttttccgg
     6301 ccggtgaaca gattgcttat tcaaattatg gttctgccct ggctgcttat atcgtggaaa
     6361 gagtatccgg actgccattt tttgaatatg tagaacaaaa catatttaat cctttaaata
     6421 tggagagaag cactttaata caaccggtac cggaaaatat tgagggagaa gtatcttatg
     6481 gttatgccaa aataaatggt gtatttaaaa aagatgaatt tgaatatata cagccttatc
     6541 cagccggttc tttaagtaca actgcttctg atatgtctaa atttatgtta gctcatttaa
     6601 acaatggatt ttttggcgga aatagaatat tggaagctga tacagtccgt gaaatgcata
     6661 gccagagctt tactcattat gaggaatttt caggtatggc ccacggcttt atagagatga
     6721 attataatgg ctacaggatt attacacacg gaggagatac ctttttattt cacagcggta
     6781 tatatttaat cccggaatta gatttaggtt tttacgtatc ctataacggc agagatgctg
     6841 ctttagccag ggattcctta ataaagaact ttttagatag atactatcca tctgatagag
     6901 atatcgttcc tgaagaagca ggcagtaact ttatggacaa ttctaaaata accgggactt
     6961 attatccgaa tagaagtaat tttacagatt atcaatctgt tgtcaggatt atcacacaga
     7021 ctaatattag attaactcag gccggcaatc ttacttataa ctttatgggg gaaaaccatc
     7081 aattagaaca aattgccccc cgtgttttct atgataaaga catcggtaat aaactatatg
     7141 cgccggaggg tgaagagata aaccgacttt atacaaacgg cccgaatggt ttgttaaagg
     7201 caaaatggta tgaaacactt aactttaacc taatatttct aataggttat gcgctgtttg
     7261 gaattgtatt tagtattgtc ttaatcaaat cattgtttaa aagatatatt agaaataagt
     7321 atattttaga aaagatcacg gtagttatat ttacagtggc cgcaatcggt ttcttcatag
     7381 cgatcggaat atttttagca aacactcatc ccttatataa tgtaccgtat ctattcttag
     7441 aggtgacacc tgctttcgat aatattctgc ttttcctctg gataatacca gttttagctg
     7501 tgattcttgt tgtaatgaat gtgaggatat ggtttaaagg tagatggaat gtaattcaga
     7561 aaggtatcta t
//
LOCUS       DATGCJ010000178         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_383999, whole genome shotgun sequence.
ACCESSION   DATGCJ010000178 DATGCJ010000000
VERSION     DATGCJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383999"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /gene="mutS"
                     /locus_tag="VKN64_08460"
     CDS             complement(<1..1946)
                     /gene="mutS"
                     /locus_tag="VKN64_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMA60309.1"
                     /translation="MAKLTPMMQQYKEIKENYPDSILFFRMGDFYEMFEEDAKLAAKK
                     LDIALTARNKGSGEKTPMAGVPAHSAESYIAELIDQDFSVAICEQLEDPSEASGLVKR
                     DVIRVITPGTIIENEILDSSENNFLTSIVQFEDKIGISYVDISTGDFFITEIAGKKLE
                     KVWDEIDRIKPREVIFDKTIEKSPKYEYLENKLDFVVKHKEKMSYEKAYNLLIDHFNT
                     QSLSGFGCDDLRAGVIAAGQVMNFLKDTQKRTIFHINKLKRYNIDDYMVLDYSTRRNL
                     ELTTTIRENKKEGSLLSIIDKTTTAMGSRLIKRWINQPLIEKERIIQRLNAVDELSDN
                     YLKMQEIQENLEEIYDLERILSKITYETANARDLAFLRTSLNKLPAIKASIKEFETEY
                     INELHENFDTLNDLYELIKESIVDDPPVTVREGNLIKEGYDEELDELRSMRDEGKDWI
                     TNLQKEERERTGISSLKVGFNKVFGYYIEVTNPNLDKVPNDYERKQTLTNSERYITPE
                     LKEKEAKVLGAEEKINDMEYELFVEVRNEISNNIERIKETATIISKIDVILSLGIVAI
                     ENNYCKPEINNSNKIEITNGRHPVVEKMVTDRFVPNDTYLDNDDNRFLIITGPNMSGK
                     STYMRQVALIILLAQIGSFIPAKRA"
     gene            complement(2059..3390)
                     /gene="miaB"
                     /locus_tag="VKN64_08465"
     CDS             complement(2059..3390)
                     /gene="miaB"
                     /locus_tag="VKN64_08465"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMA60310.1"
                     /translation="MVENKKYYIKTYGCQMNEHDSEKIAGMLEEMGYNPTDELEKADL
                     IFLNTCTVRENAELKVFGKVGSLKRLKRANPDLIIGIGGCMMQVEEPVQKIKEKFPHV
                     DLVIGTHNLHKLPELVSEIEESDQRIISVWDQEKGIIPDLPSVRKSDFQAWVTIMQGC
                     DNYCSYCIVPYVRGHERSRLKENIINEVENLIEDGVIEITLLGQNVNSYAQDLEADYD
                     FADLLAELDEIEGLKRIRYMTSHPRDFNEKTIKTIADSNKVCEHFHLPIQSGSNDVLY
                     EMNRGYTREDYLAMIDNIKEHFDHYSITTDIIVGFPGETDEDFQDTLDIVKEVKFDMA
                     FTFKYSKRTGTPAAKKDNQISEEVKDRRLQKLMKVQNKISLRQNKKLEGKNLEVLVEG
                     ESKNNPATFMGRSRTNKLVIFPRQEGLIGKVVKVKIDNAQSWTLYGDVIET"
     gene            complement(3517..>4231)
                     /locus_tag="VKN64_08470"
     CDS             complement(3517..>4231)
                     /locus_tag="VKN64_08470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HMA60311.1"
                     /translation="LILGLPYVASLQGDAITFNLFIRFLGLNGAISAVVIFFGLFFSK
                     NIGLDIPILRAYTAKKKIPKDKLNYLYKWAPILGLLFGILIYSLDLLFAPYLKDLSEA
                     ATQAGYQFVWWKSLLAAFYGGIFEEILMRLFSLSFYAWIITWVLSKFRKEEMKENKTV
                     IWFAIIFSAISFGVGHLPTLMAMTELTTILVIRTVLLNALPGIFFGWLFYKKGLEHAM
                     VSHFFADISLHLIMPLFFT"
BASE COUNT         1241 a          832 c          648 g         1510 t
ORIGIN      
        1 gcccttttag ccggaataaa gcttccgatt tgggctaaca gtataattag agcaacctgt
       61 ctcatatagg tcgatttacc ggacatattg ggtcctgtaa taattaaaaa cctattatca
      121 tcattatcaa gataggtatc attgggtaca aaacggtctg tcaccatctt ttccacaaca
      181 gggtgtcggc cgtttgttat ctcgatttta ttagaattat tgatttccgg tttacagtaa
      241 ttattttcta ttgctactat gccaagagat agaattacat ctattttact gataatagtg
      301 gctgtttcct taattctttc aatgttattt gatatttcat ttctaacctc gacaaagagt
      361 tcatactcca tatcatttat cttctcttct gcccctaaca cttttgcctc tttttcttta
      421 agttcaggtg taatatatct ttcgctgttg gttagggttt gttttctttc atagtcattg
      481 ggtactttat ctaaattggg attggttact tcaatatagt agccgaagac tttgttaaag
      541 ccaaccttta aagaagaaat tccggtccgt tccctctctt ctttttgcag gttggtaatc
      601 cagtctttac cttcatcccg catcgatctc agttcatcta attcttcgtc ataaccctct
      661 ttgattaaat ttccttccct taccgttacg ggtgggtcat caacgataga ttctttgatt
      721 aactcatata aatcatttaa agtatcgaag ttttcatgaa gttcatttat atactcggtt
      781 tcaaattcct tgatactggc ttttatggcg ggtagtttat ttaaagaagt tcttaaaaag
      841 gctaaatccc ttgcattggc cgtttcatag gttatcttac ttaatattct ttctaaatcg
      901 taaatttctt ctaaattttc ttggatttcc tgcatcttaa ggtagttatc acttaattca
      961 tctacggcat ttaatctctg tatgatccgt tctttttcaa ttaagggttg attgatccag
     1021 cgtttaatta atctagagcc catggcagtg gtagttttat caataatact taagagactg
     1081 ccttcttttt tgttttcccg tattgtggtg gtaagttcaa gatttctccg ggtagaataa
     1141 tctaatacca tataatcatc tatattatac ctttttaatt tattaatatg aaaaatagtc
     1201 cttttttggg tatctttaag gaagttcatg acctgtccgg ccgcaattac accggctctt
     1261 aagtcatcac agccaaaccc agatagagat tgggtgttaa agtgatctat taggagatta
     1321 tatgctttct catagctcat tttttcttta tgttttacga caaagtctaa tttgttttct
     1381 aaatattcat atttagggga cttctcaatc gttttatcaa agataacttc ccttggtttt
     1441 atcctatcaa tttcatccca gaccttttct aactttttac cagcaatttc ggtaataaaa
     1501 aagtcaccgg tcgagatatc cacataagag atcccaattt tatcttcaaa ttgaacaatc
     1561 gaggttaaga aattattttc agaagaatca aggatctcat tctctataat tgtaccgggc
     1621 gtaataacac ggattacatc tcttttgacc agaccgcttg cttcactagg atcttctaac
     1681 tgttcacaga ttgccacact aaaatcttga tcaattaatt ctgcgatgta tgattccgcc
     1741 gaatgggccg gcacccctgc catcggggtc ttctcgcctg aacccttatt acgggcagtt
     1801 aaggcaatat ctaacttttt tgcggctaat tttgcatctt cttcaaacat ctcataaaaa
     1861 tcacccattc taaaaaatag aatggagtcg ggatagttct cttttatttc tttatactgc
     1921 tgcatcatgg gagttaactt ggccaaaata ccacctcatc tatgttaaaa aatacttatc
     1981 tatatagact atacatacta aataatatta tagcataaga tactattcta ataaagaaaa
     2041 cccgaaagat taaatctttc aggtttctat cacatcaccg tataaggtcc agctctgtgc
     2101 attatctatt tttactttaa ctactttacc tattaaacct tcctgacgcg ggaagataac
     2161 cagcttattt gttctagacc gtcccataaa agtagcggga ttatttttgc tttctccttc
     2221 taccagaact tctaagttct ttccttctaa ttttttattc tgtcttaaac ttatcttatt
     2281 ttgcaccttc attaatttct gcagccgtct atctttcact tcttcactta tctgattatc
     2341 ttttttagca gccggggtac cggtcctttt agaatattta aaggtaaatg ccatatcgaa
     2401 tttaacttct tttacaatat ctagtgtatc ttgaaaatct tcatcggttt cgccgggaaa
     2461 tccgacgata atatcggttg taattgaata gtggtcgaaa tgttctttaa tattgtcgat
     2521 cattgctaaa taatcttctc tcgtgtagcc tctattcatc tcatataata catcattgct
     2581 accggactgg ataggcaggt ggaaatgttc acacacctta ttactatcag caatagtttt
     2641 gattgtcttt tcattaaagt ctcgcggatg tgaggtcata tatctaattc tctttaagcc
     2701 ttcaatttca tctaattcag ccaaaagatc ggcaaaatca taatcagctt ccagatcctg
     2761 ggcataggaa tttacatttt gtccaagtaa agtaatttca atgaccccat cttcgattaa
     2821 attttcaact tcattgatga tattttcttt cagcctgctt ctttcatggc cccgtacata
     2881 agggactata cagtaactgc agtagttatc acagccctgc attattgtta cccaggcctg
     2941 aaagtcgctt ttacggaccg aaggtaagtc gggaattata cccttttcct ggtcccagac
     3001 cgagataatt ctttgatcgc tttcttcaat ttcactgaca agttccggta atttatgcag
     3061 attgtgagtt ccgataacta aatccacatg agggaacttc tcttttattt tttgcacagg
     3121 ttcttcgacc tgcatcatac agcctccgat tccaataatt aaatcgggat tggctctttt
     3181 aagtctcttg agggaaccga ccttgccgaa tactttcagc tcggcatttt cccggactgt
     3241 acatgtattt aaaaatatta aatcagcctt ttctaattca tcggtaggat tatatcccat
     3301 ctcttctaac ataccggcta tcttttcaga gtcgtgttca ttcatctgac agccgtatgt
     3361 tttaatatag tactttttat tttcaaccat aagaattctc cttaatttat ctgatagtta
     3421 tttttagcct aagtagtatt ataacacagg agaattgctt tttaaatatt aataacaaaa
     3481 aaagtttagt aatttaataa gaaataacta ttagtattat gtgaaaaaca agggcatgat
     3541 taaatgtagt gaaatatcag caaaaaagtg tgataccata gcatgttcta gtcctttctt
     3601 ataaaataac cagccgaaga aaatacccgg tagagcattt aaaagaactg tcctgataac
     3661 caggatcgtt gttaattcag tcatggccat taaagttggc agatgtccta ccccaaatga
     3721 aatagctgaa aatattatag caaaccagat cacagttttg ttttctttca tctcttcttt
     3781 tctgaatttg gataaaaccc aggtgataat ccaggcgtaa aaggataaag aaaatagcct
     3841 cattaatatt tcttcaaaga taccgccata aaaagccgcc aataatgatt tccaccagac
     3901 gaactgatag ccagcctggg tagcggcttc acttaaatct tttaaataag gtgcaaacaa
     3961 tagatcaagt gaatatatta atataccaaa gagtaaacct aagatagggg cccatttata
     4021 taaatagttc aatttatctt tcggtatttt cttctttgct gtataagctc ttaaaattgg
     4081 tatatcaagc ccaatatttt tactaaaaaa tagtccaaaa aagataacta cagcagagat
     4141 agctccattt aaacccagga atctaataaa caaattaaaa gttattgcat ccccctgtaa
     4201 acttgctaca taaggtagac caagtattaa a
//
LOCUS       DATGCJ010000179         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_339379, whole genome shotgun sequence.
ACCESSION   DATGCJ010000179 DATGCJ010000000
VERSION     DATGCJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339379"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VKN64_08475"
     CDS             complement(<1..1229)
                     /locus_tag="VKN64_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60312.1"
                     /translation="MVINLQMEQGKTQEFELELTPEENQETVKLNLYYPRQQLTGTLS
                     YEEENPEKHSVLSWLDLPEEVIVPPGEKISVTVEVSVPYGVKGTHTAVIMVEPITEDV
                     EGGITFQIRYAVRVNINVNSPGLRRRAEIQEFKLVANEEDKPVLQAMIKNPSSLSYNA
                     AGEVTIRDENRNLIERVTIRSQYAAQGGRDETTIYPGSEVIFSGNVTEPLPAGTYDMQ
                     LFLYYADGRQVIKRKTIEIGDQYIDPENLEYIEVKPEMISEELRRGGAHTEAINIRNR
                     MGDPLNIKIGTKQISSDYKRSLLNKFKLELRGDQQFKLEGRRSQRPVLIARAPREDIE
                     DGGYYDSLQIAVFDPKTEERLQVKEIPMRFVVGEDYEYNGEVQGITTQRMENEVLLST
                     PVLNTGDIHFSPQARVY"
     gene            complement(1485..2132)
                     /locus_tag="VKN64_08480"
     CDS             complement(1485..2132)
                     /locus_tag="VKN64_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60313.1"
                     /translation="MRKLGILVMVIVLVFGVSVIGYAVSQHGADEIGVNVNVTDAAYV
                     DVEDENLTLNVSELNTLNASEYTTVKVTANNDLNVRFEENLSRKFYNEYFKGETSSNR
                     VWRWDTYAMNTTTGKGGYGDYDNWVVSPDVQVKDAWENHPSDFEGPMYQHNDGFGRIL
                     NVSMGSNEYEVRAATDWRSHDDQGNELPWYLVESGTSIDGEITITVEAGTDDISF"
     regulatory      complement(2160..2247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(2383..3450)
                     /locus_tag="VKN64_08485"
     CDS             complement(2383..3450)
                     /locus_tag="VKN64_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60314.1"
                     /translation="MKNKTRWRYNKLGLSILVLFLITIISLPVMAENGNGNSGVSSPP
                     DYKVSRPVVVIKVTPPASSNNISQSVKLYDSMEDIESQDYLQGNMSVEWLTNNSEGSH
                     SEENDDSGEDLKFEIEDDLTLNKQNSESSFGFTSSNNSENVISPNYILVKRNSDSNYK
                     SLDKRVEVLNRNSNKSELEFRLDSNIFNDYNWKNIEAGVYTGQITPNIGLPGNSNKRL
                     KIIVIVESVVNIEIPEELNLTVEKPVEDESAAIEWRVKTNNNNIRIEFESEGIKLEDT
                     EERNIDEEHIDYKEFFRYYVENDSVNSKHEFVPGGNSEVNCIDGELKLRYSPNDYGDE
                     EWYELMAGKYKDTVTITVTGN"
BASE COUNT         1069 a          721 c          424 g         1276 t
ORIGIN      
        1 aagtaaactc tggcctgagg actaaaatga atatcccctg tgtttaagac aggcgtactt
       61 aataatactt cattttccat cctttgggtg gttataccct gtacttctcc gttatattca
      121 taatcttcac ctacaacaaa ccgcattgga atctctttta cctgaagtct ttcttctgtc
      181 tttggatcaa agactgcaat ttgcaggcta tcatagtaac ctccgtcttc tatatcttct
      241 ctgggagctc tagcaattaa tactggcctc tgggaacgtc ttccctccag tttaaactgc
      301 tggtctcccc ttaattctaa cttaaattta tttagaagag aacgtttata atcggaagaa
      361 atttgtttag tgcctatctt aatatttaac ggatctccca ttcgattacg aatgttaatg
      421 gcttctgtat gtgctccacc ccttctcaac tcttcagaaa tcatctccgg tttaacttct
      481 atatattcca gattttctgg gtctatgtat tgatccccaa tttctattgt tttcctttta
      541 attacctgtc ggccgtcggc ataatataag aataattgca tatcataggt gcctgcaggt
      601 aaaggttctg ttacatttcc ggaaaaaata acttctgaac caggataaat ggtggtttca
      661 tctcttccgc cctgagctgc atattgactt ctaatcgtca ctctttctat taaattacgg
      721 ttttcatctc taattgttac ctcaccagca gcattatagg ataaagaaga gggattttta
      781 atcattgctt gtaatacagg tttatcttct tcattagcta ccaatttaaa ttcttgaatt
      841 tcagctcttc ttctcaaacc aggagaatta acattaatat tcactcttac tgcataacga
      901 atctgaaaag ttattcctcc ttcaacatct tcagttatag gttcaaccat aataactgca
      961 gtatgagttc ctttaacacc ataaggaact gaaacttcaa ctgtaaccga gattttctcg
     1021 ccaggtggaa ctattacttc ttctggtaaa tccaaccaac tcagcactga atgtttttca
     1081 ggattttctt cctcataact aagagtaccg gttagttgtt gcctggggta atataaattg
     1141 agttttactg tttcttggtt ttcttcgggg gttaattcta gttcaaattc ctgagttttt
     1201 ccctgttcca tttgcaaatt aattaccaaa ggacgaacac cgactgcttg gatattatat
     1261 gaaaagcaga aaaaaaatat taaaaatatt atacttatat aaaatatatt atttttatat
     1321 ctaaacattt atttgaatct tcctccccat gaaaaataat gtatgaaaat cattgtaaaa
     1381 tatattatag catttgtcta aaagaaaaca tttgggaccg aactatattg ttgtccggcc
     1441 cctttaaatt cagtatttat attttttgtt caataaatga tacattaaaa tgatatatca
     1501 tctgtacctg cttcaacagt tattgttatt tcaccatcaa tagatgttcc agattcaact
     1561 aaataccaag gtaattcatt tccctgatca tcatgacttc tccaatcagt agctgctctt
     1621 acttcatatt cattggaacc cattgaaaca ttaagaattc ttccgaatcc atcattatgt
     1681 tgatacattg gtccctcgaa atctgaaggg tggttttccc aagcatcttt tacctgaaca
     1741 tctggagata ctacccaatt atcataatct ccatatccac cttttccagt tgttgtattc
     1801 atagcataag tatcccatct ccatactcta ttagaacttg tttcaccttt aaagtattca
     1861 ttataaaatt tcctacttaa gttttcttca aatcttacat tcaaatcgtt attagcagta
     1921 acttttacag tagtatattc ggaagcattt aaagtgttta attcactaac atttaatgtt
     1981 aaattttcat cttctacatc tacataagct gcatcagtta cattaacatt tacaccaatt
     2041 tcatctgcac catgttgaga aactgcataa ccaataacac tcactccaaa aaccaatacg
     2101 attaccataa ccaatatacc taactttctc atcataaatc ccccttgatt ggattttttg
     2161 ccctttatgg gtggtttcac tactaactcc ctgctacccc gttagtgacc aattaaagag
     2221 cagcagctca ccgcttccaa taagggtaag taataaaata agccttaacc aacattaccc
     2281 gttctctcca gcaaaacagt cttttcacta ttagttacca aatttacata tcatctatag
     2341 ggcttttcat taccccctgc tttaattaaa aatttgaatt aactaatttc ctgtaacagt
     2401 tatagtaact gtatctttat atttgcctgc cattaactca taccattctt catctccata
     2461 atcatttggt gaatatcgta attttaactc accatcaata caattgactt ctgagtttcc
     2521 tcctggaaca aattcatgtt tagaattaac actatcattt tccacatagt atctgaaaaa
     2581 ttctttataa tctatgtgct cttcatctat atttctttct tcagtatcct ctaatttaat
     2641 accctctgat tcaaattcaa ttcttatatt attattatta gtcttaactc tccactcaat
     2701 agcagcagat tcatcttcta caggtttttc cactgttaaa tttagttctt caggaatttc
     2761 aatatttacc acagattcta caataacaat aatttttaaa cgcttattag aattaccagg
     2821 cagtccaata ttaggagtta tttgtcctgt ataaacacct gcttctatat tcttccaatt
     2881 ataatcatta aaaatatttg aatctaatct aaactcaagt tctgacttat tagaattcct
     2941 atttaatact tcaactcttt tgtctaaact tttataatta gaatcactat ttctttttac
     3001 taaaatataa ttcggactta taacattttc agaattatta ctactagtaa agccaaaact
     3061 gctttctgaa ttttgcttat ttaatgtcaa atcatcttct atttcaaatt taagatcctc
     3121 ccctgaatca tcattctcct cagaatgaga accttcacta ttattagtaa gccattcaac
     3181 gctcatattt ccttgcaaat aatcttgaga ctctatatcc tccatactat catataattt
     3241 tactgattga gaaatattat tagagctggc aggtggagta acttttatta ctactaccgg
     3301 acgagaaact ttgtaatccg gtggtgaact gacacccgaa tttccattac cattttcagc
     3361 cataacaggt aaacttataa tagttattag aaaaagaact aatatagaca aacctaattt
     3421 attatatctc catcttgttt tattcttcat ggacaattct ctcccttcgg agattaatta
     3481 tgaaataaat
//
LOCUS       DATGCJ010000180         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_515110, whole genome shotgun sequence.
ACCESSION   DATGCJ010000180 DATGCJ010000000
VERSION     DATGCJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515110"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VKN64_08490"
     CDS             <1..657
                     /locus_tag="VKN64_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMA60315.1"
                     /translation="MENATNLQYRFGMPGPEIKETEIKNIFKTIKNINQFPKYFVISG
                     SIPQGVSPDIYAAMAKIAKEKGAKVVVDVSGEPLKEVMKEGVFLIKPNMGEFQELVGR
                     ELKEEEEIKKEAYKLVNDNCCQNVVISLGAGGALMVNQETARFMRPPTVPIKSKVGAG
                     DSMVAGIVLSLARGKDFQEAVLYGLAAGSSAVMTPGTELCRKEDTDKLYEKMYQEIFV
                     "
     gene            754..2757
                     /locus_tag="VKN64_08495"
     CDS             754..2757
                     /locus_tag="VKN64_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA60316.1"
                     /translation="MIKKNTYNKNDLEKILDCLPGMAYQCLYDDDWTMYFVSEGCKHL
                     TGYREEEILENAALSYKDIIHEDDRGKVNKKVKKGVKDHNSYNIEYRIFTKEGEMKWV
                     WEQGRAIYDNNDNVKYLLGFITNITNRKLNYNELKDTKERLELAVQGANLGVWDWNIK
                     TGRININKNWTDMLGFDQDDIDPIVDEWERLIHEEDKDKVYDNLNEHLAGESKYYSVE
                     HRLATKEGGYKWVRDIGKVFKRDKAGNPLRAAGIHLDIDDAKRIEKQLRVSEEKFRTY
                     IENAPIGVFVVDYEGNILEVNDHAAKMTGYGEEELTNLTLFELTARSEKETKYYLRNF
                     KRKGQIEIEGKFIKKNDEQFYAKVNGIVIDEDKFLGFVEDIDKRIKMRNRLKRQKAYF
                     EQLFNESTEGIVLLNNKGIILKVNNHFLEIYGYDRDEVVSENIDDLITPPDRKEEGIY
                     YTEEVMAGNDIKEESVKVTKSGERIHVSIHAFPIKLDEGQIGIYGIYNDITERKKEEE
                     KRRYLSFHDQLTDLYNRRYFENEMERLGGSRKTPISMIIADIDGLKEVNDTKGHQWGD
                     KHIKNTADIISGVTRQEDIVARIGGDEFAILLPETDEKGTHKVIDRIKESIEFRNKSA
                     EVPLSISIGYSVQENKDGNLEENFKKADKMMYHRKRNKKHNSC"
     gene            3820..>4559
                     /locus_tag="VKN64_08500"
     CDS             3820..>4559
                     /locus_tag="VKN64_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMA60317.1"
                     /translation="MRKLTTFILTLLLLLMFSTVMYAAIDVGNVPQDVEDVFKREVEG
                     VEVESWITNLNIPWQLIFLPDSSRALVTERTGTIRLIENGNLVDEPYAQPEVAAVGEG
                     GLMGIAYHPNFPAEPYIYIMYTYRNQNGTLYNRVTRLTDRGTKGDSEEVILDQIPASR
                     VHNGGRIAFGPDDKLYVTTGDTWNRTIAQSMDNLGGKILRLNSDGSIPEDNPYDGSYI
                     YSLGHRNPQGLAWHPVTGHLFITDHGPSG"
BASE COUNT         1786 a          634 c          938 g         1201 t
ORIGIN      
        1 atggaaaatg cgactaacct acagtatagg tttggcatgc caggacctga gattaaagaa
       61 acagaaatca aaaacatatt taaaaccatt aaaaatataa atcaattccc taaatatttt
      121 gtgatcagcg gcagtatccc ccaaggtgtt agccctgata tttatgccgc aatggctaaa
      181 attgccaagg agaaaggggc taaagttgta gttgatgtct ctggcgaacc tttaaaagaa
      241 gtaatgaaag agggagtctt tttaataaaa cccaacatgg gggaattcca ggaattagta
      301 ggtagagaat taaaagagga agaagaaatt aaaaaagaag catataaatt agtcaatgac
      361 aactgctgtc aaaatgtcgt aatctccctg ggagccggtg gcgccctgat ggttaatcag
      421 gaaactgccc gtttcatgcg gcccccgacc gtaccgatta aaagtaaagt aggtgcaggt
      481 gacagcatgg tagccggtat tgtattaagc ctcgccagag gtaaagattt tcaagaggca
      541 gtcctttacg gcctggcagc aggctcttca gctgtaatga caccgggaac agagctctgt
      601 agaaaagaag atactgataa attatatgaa aaaatgtatc aagaaatatt tgtataattc
      661 ccataattaa tagaattatc tataaaattt tgttatcatg aatattagta tttaaaattt
      721 ttattcaatt aaattaatca aaaggtgaat attatgataa aaaagaatac atataataaa
      781 aacgacttag aaaaaatatt agactgtttg ccgggtatgg cctaccagtg tctctatgat
      841 gatgattgga ctatgtactt tgttagtgaa ggctgtaaac atttaaccgg ttatcgggaa
      901 gaggaaattt tagaaaatgc agccctttcc tataaagata taatacatga agatgatagg
      961 ggtaaggtta ataaaaaggt aaaaaagggt gttaaagacc ataattcata taatattgaa
     1021 taccggatct ttactaaaga gggagagatg aaatgggtct gggaacaggg tagagcaatt
     1081 tatgacaata atgataatgt aaaataccta ttgggcttta tcaccaatat tacaaacagg
     1141 aagttaaact ataatgaact caaagatacg aaagaacgtc tggaattggc tgtacagggt
     1201 gccaatctgg gggtttggga ctggaatatt aaaacaggca gaatcaatat aaataagaac
     1261 tggactgata tgttgggatt tgaccaagat gatatagatc cgatcgtaga tgagtgggaa
     1321 agacttatcc atgaagaaga taaggataag gtctatgata atttaaatga acacctggcc
     1381 ggagaatcta aatattattc tgttgaacac cgccttgcta ctaaagaagg cggatataaa
     1441 tgggtcaggg acatcggtaa ggtttttaaa agagataaag ccggcaaccc cttaagagcg
     1501 gctggtattc atcttgatat tgatgatgca aaaagaatag aaaaacagtt aagagtcagc
     1561 gaagagaagt tcaggaccta tatagaaaat gctcctatcg gagtttttgt cgtagattat
     1621 gaaggtaata ttttggaagt aaatgaccat gccgcaaaaa tgaccggcta tggtgaagaa
     1681 gaattaacaa acctaactct ttttgagctg actgcccgta gtgagaaaga gactaaatat
     1741 tatcttagaa atttcaaaag aaaaggacag atagagatag aaggaaagtt tattaaaaag
     1801 aatgatgaac agttctatgc taaggtcaat ggaatagtga tagatgaaga taagtttttg
     1861 ggctttgtgg aagatattga taagaggatc aagatgcgca acaggctcaa aaggcagaaa
     1921 gcctactttg aacagttatt taatgaatca acagaaggaa ttgttctatt aaataataag
     1981 gggataatat taaaagttaa taaccacttc ttagaaatat atggttatga tagagatgaa
     2041 gtagtgagtg aaaatattga tgatctcata actcctccgg atagaaaaga agaaggaata
     2101 tattatactg aagaagtaat ggccggcaat gatatcaaag aagaatcggt caaggtaacc
     2161 aagtccggtg aaagaatcca tgtatctatc cacgccttcc ccatcaaact ggatgaaggt
     2221 cagattggta tttatggtat atataatgat attactgaac gtaaaaaaga agaagaaaaa
     2281 agaagatatc tttcttttca cgatcaacta accgatttat ataatagacg gtactttgaa
     2341 aatgaaatgg aaaggcttgg cggttcccgt aaaactccta tcagcatgat aatagccgat
     2401 atagatggtt taaaagaggt taatgataca aaaggccatc aatggggtga taagcatatt
     2461 aaaaatacgg ctgatattat ttccggggtg accaggcaag aagatattgt ggcccggatc
     2521 gggggagatg aatttgctat cctcttacct gaaaccgatg aaaagggaac tcataaagtt
     2581 atagaccgga tcaaagaaag tatagagttc aggaataaat cggctgaggt accattaagt
     2641 atttcgatcg gttattctgt tcaagaaaac aaggacggga atctggaaga aaactttaaa
     2701 aaggccgata aaatgatgta ccataggaag cggaacaaaa aacataacag ctgttaaatt
     2761 ctaaagaaga tagaataaac taatcccact tttcagtggg atattttttt atgcagaaaa
     2821 gttaatctat cgattaaatt aattaataaa gttaataaaa aattaataaa ttattaatag
     2881 atattaataa aatagctcaa ttaatgtgat attattaata taaagcgtct aaaaagggtt
     2941 aaaattaaca aatattaata atataattaa aatatttaaa aaaataatta aaaatattaa
     3001 tattttactt gacaaacatt aaaataatgt ttataatata actagaataa agaaataaac
     3061 aaaaaaggag gagttaaaat gtttaacaac aaaaagttca gattgatcga agacagaaac
     3121 tggcgtaaag aaattaaact gaacaagaag caaaacagag ttgccgacga gtatattaat
     3181 cagtacttct aaagaaaaaa atacataaat aaaaaggggg tagaaaaatg tttggtaaaa
     3241 aattaaatga tttcttttcc ttagaaaata gaaactttaa aagagaaaga aaagaaaaat
     3301 taagagatca gatccaaatc gaagagtaca gaagtgaatt aatgtaagag acgataaaaa
     3361 aaacaaaagg aggttagaaa tatgagtaac ctaaaaagat tcttgagatt agaaaacaca
     3421 agttataaca atcagtataa agaaaacaga agagaaaata ctagattaga atcatataaa
     3481 gtaatgggtc tataaaaaat aaaaaaggag gacaaactag atgatgataa taataataca
     3541 aagagatcta aataaataac gaacataagg atataaaaga ttgataataa ttaaaaggtg
     3601 gtgaacagat aaatgattgc taaagataat ccttatgtta aaaaatcaaa gtcggaattt
     3661 gatttttaag tccctctcta atgaaagaaa ttagaaggga cttttttaat gctttaagtt
     3721 aactatttta cttcatttga gcataaaaaa atgtattctt taaacaagga ttatattttt
     3781 aaatgttcaa atataataaa ggaaagtgag gaaaaaatga tgagaaaatt aactactttt
     3841 atattaactt tactcctgtt gttaatgttc agtacagtaa tgtatgctgc tattgatgtc
     3901 ggtaatgtac cgcaagatgt cgaggatgta tttaaaagag aagtagaagg tgtcgaggtt
     3961 gaaagctgga ttaccaacct gaatatcccc tggcagctta tatttttacc ggacagcagc
     4021 cgggcgcttg tcaccgaaag aaccggtacg attagattga tagaaaacgg taatttagtt
     4081 gatgagccct atgcacaacc agaagtagca gctgtcggtg aaggtggact catgggaatt
     4141 gcttaccatc cgaactttcc ggccgaacca tatatctata ttatgtacac ctacagaaat
     4201 caaaacggga ctttatataa cagagttacc cgtttaacag accgcgggac taagggcgat
     4261 agtgaagagg taattctaga tcagattcca gccagccggg tccacaatgg tggacgaata
     4321 gcctttggac cggatgataa actctatgtt acaaccggtg acacctggaa ccggactatt
     4381 gcccagagca tggataatct gggtggtaaa atcctgagat taaattcgga cggctcaata
     4441 cctgaggata atccttatga tggttcctat atctacagct taggacaccg caacccgcag
     4501 ggcttagcct ggcaccccgt gaccggccac ctctttataa ccgaccacgg accttccgg
//
LOCUS       DATGCJ010000181         6908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_605089, whole genome shotgun sequence.
ACCESSION   DATGCJ010000181 DATGCJ010000000
VERSION     DATGCJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605089"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1424
                     /gene="pfkA"
                     /locus_tag="VKN64_08505"
     CDS             426..1424
                     /gene="pfkA"
                     /locus_tag="VKN64_08505"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMA60318.1"
                     /translation="MKKIGILTSGGDAPGMNPAIRSVVRTAVYRNKEVIGFYNGYSGL
                     LDGKYTEMTRRSVGDIIHRGGTILRTARCREFETKEGREKAYQKIEELDIGGLVVIGG
                     NGSLTGAKLLNEEFGVPVIGIPATIDNDLAMTQFCIGFDTAMNTVLDALNKVRDTATS
                     HERTFVVEIMGRDAGYLALYTGMAGGAESILIPEIEFRLDDIINNCKAGFERGKLHNI
                     IVVAEGAGKKLREESGDDRNIGTIIGNHIRKELSVETRVTILGHLQRGGSPTAMDRII
                     STRMGAKAVDLLLDGEEDRMVSFDGKNVIDVDLNKALTKEKNIDMDIYNLARILSL"
     gene            1440..3200
                     /gene="pyk"
                     /locus_tag="VKN64_08510"
     CDS             1440..3200
                     /gene="pyk"
                     /locus_tag="VKN64_08510"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMA60319.1"
                     /translation="MRKTKIVCTLGPAANDKETLKNLVEAGMDVARLNFSHGDHEEHG
                     NRIEMVKEVEKEIDKPIGIILDTQGPEIRTGKMKNDKVTLKNNAEVTITTKEVVGTQD
                     KFQIKYDNLPQDINNDSTILLDDGLLELKVKEIKKKEEEIVCTVINGGDLGSHKGVNI
                     PGIKTTLPAITEKDKKDIIFGIKHNIHFIAASFIRKASDVLEIRKVLEEEWAQDIKLI
                     SKIESEEAVRNIDEIIEVSDGIMVARGDLGVEMAAEKVPIIQKMIIKKCNEAAKPVIT
                     ATQLLDSMIRNPRPTRAEASDVANAIFDGTDATMLSGETAIGSFPVQAVKTMANIARE
                     TELSDTYEAQLSSSKAIRADTVTEAISIGSCEIASGLYADAIITATGSGSTARMVSKC
                     RSMTPIVAVTPNDMALHNLTLSWGVYPTMAADSTSTDEMMDNSINVALENGLVDEGDL
                     VVLTAGVPVGTPGTTNLIKVDIVGDPIVEGQGIGRKIIHGPVKIVNSANEALEKVEEG
                     DILVARSTNNQYNEAIQKSAGIIAVEGGVTSHSAVVGIQYNKPVVVDAVGIIDILVDG
                     ETVTIDSVRGHIYKGKVNLK"
     gene            3294..4163
                     /locus_tag="VKN64_08515"
     CDS             3294..4163
                     /locus_tag="VKN64_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMA60320.1"
                     /translation="MSIKTKKVIKNYIIIAAGAIMTALSLVLFTVPNKIAAGGVSGIA
                     TILYHMFGFNVGIVILIINIPLFLVAVYVLGGSIGIKTLWGIIVMSVSVEVLTPLLNP
                     LTNEVILASIYGGVLGGVGMGFVFRGGGTTGGTDLIAALTSYFFPHFSIGQGLFLIDG
                     IVITLAGIVFDVELALYAAITIFVSTKLIDIIQEGFNISKSVFIISDRSQEIKDKILD
                     NMRRGVTALRGYGGYSGREKDVLLVTINRAEITKLKNLIRDIDEEAFVIMNEAHEVLG
                     EGFKRINERAINE"
     gene            4257..4532
                     /locus_tag="VKN64_08520"
     CDS             4257..4532
                     /locus_tag="VKN64_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60321.1"
                     /translation="MSIVEEFNLEKIEEGKTYEMYEGSYSSEEEIERKLELAGYKFIS
                     EERRIYVTKVYADDMEKRIAVLDSGNIRIWDFDSEEYYEMELDHHRE"
     gene            4647..4721
                     /locus_tag="VKN64_08525"
     tRNA            4647..4721
                     /locus_tag="VKN64_08525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4679..4681,aa:Gly,seq:ccc)
     gene            4812..5291
                     /locus_tag="VKN64_08530"
     CDS             4812..5291
                     /locus_tag="VKN64_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60322.1"
                     /translation="MSNEYDFVTVKIKINKLNKMLPEITHKREKEFLQKHMPRYLHFY
                     DYFLKKLFPFLSDFKEDIAWKVIHNGMSFYVSKDRPQDGMFMSVAFQANNKLKFSFYY
                     EEDEEDSDLSRESREGRNEDHMWYYVEDLEFDKELAKLIVQSFKEKVKYLNQKDNEE"
     gene            complement(5337..6068)
                     /locus_tag="VKN64_08535"
     CDS             complement(5337..6068)
                     /locus_tag="VKN64_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HMA60323.1"
                     /translation="MNIGAFLDIDGTLYRGSLMIQHFKKLIKYEVLDPLIWHSQVKKT
                     YDEWKKRHGYYEDYLEELAEIYINELKGMKISDLDFVNEQVINLSGDIVYRYTRCIVN
                     YHLKQNHKVFFISGSPEFLVEKMAAKYNITDYRGSKYLLDNNGHFTGEVIKMWKSNNK
                     EKVLNEFVSDYKIDLEKSYSYGDTQGDYSMLQMTGNPVAFNPNYDLISCIKEDPDLKE
                     KAEVVIERKDNIYKMNPDVEILNIE"
     gene            complement(6079..>6908)
                     /locus_tag="VKN64_08540"
     CDS             complement(6079..>6908)
                     /locus_tag="VKN64_08540"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMA60324.1"
                     /translation="SSKAIQRGVTQLGSLGSGNHFVELQYVEKVFENDTDLKEGQITV
                     MIHTGSRGFGHQIAKDHIDQAVNTNHKFNFKFPVKNLACVPIESHEGRSYLSAMGCAA
                     NFAYANRQLITHKVRNIFADLYPNQSLPLFYDLTHNMARFEKQKINDKEDEYLVHRKG
                     ATRLKPNSFALLPGSMGTASYIIKSANSESTEKSLNSIAHGSGRVMGRREAKRSITNQ
                     EHQNAIKEVEVTSSSGDSLLDESPLVYKNIEDVVESMIETELALPVVRLKPLAVLKG"
BASE COUNT         2337 a         1013 c         1374 g         2184 t
ORIGIN      
        1 cctggctttt atcttattta ttagataatt accgctcatc tttaatggca tttttaaccg
       61 gtcttatatt aggttcgacc agaagtgtta ttcccgctca atttaatatt attgcagttt
      121 taggtttagt attaggtata atggttatct attatcttct caggatagag tgaacctctc
      181 cacctaaatt acaaatttag atggtgtttt ctttgtaagt ttttagataa aataagttaa
      241 tttcatgtaa acatttattt agaacattta attttaatca ttgtcaagcc tgttataaca
      301 gtatataata aaataataaa catatgttta ccacctatat gtaatttctt gacattgtca
      361 taaaaagatt atattctgat aatatgtatt tatagaataa ttattaactg taaggaggca
      421 ttactgtgaa aaagattggt attctgacta gtggtggaga tgctcctggt atgaatccgg
      481 ctataagatc agtagtaaga acagccgttt accgaaataa agaagtaatt ggattttata
      541 atgggtacag tggtttatta gatggtaaat acacagaaat gacaagaagg tctgtcggtg
      601 atattattca ccgcggagga accatcttga gaacagcccg ctgtagagaa tttgaaacaa
      661 aagaaggtag agagaaagcc tatcaaaaaa tagaagaatt agatattgga ggtctagttg
      721 taataggtgg taatggatct ttaaccggtg ccaaattact gaatgaggaa tttggtgttc
      781 cagtaattgg tattccggcc acaattgata atgaccttgc tatgacccaa ttttgtatag
      841 ggtttgatac agccatgaat acggtcttag atgccttaaa taaagtaaga gatactgcta
      901 cttctcatga gagaacattt gtagtggaaa tcatggggag agatgctggt tatcttgccc
      961 tttataccgg aatggccggg ggagcggaat ccattttaat accagaaatt gaatttagat
     1021 tagatgatat tataaataac tgtaaagcag gctttgaaag aggtaaatta cacaatatta
     1081 tagtagttgc ggaaggggcc ggtaaaaaat taagagaaga gtccggtgat gacaggaata
     1141 ttggtacaat tattggtaat catatccgaa aggaactttc tgttgaaaca agagtaacta
     1201 ttttaggtca cttacaaaga ggtggttctc ccactgctat ggatagaatc atatctacca
     1261 ggatgggggc aaaagccgta gatctattac ttgatggtga agaggatagg atggttagct
     1321 ttgatggtaa aaatgtaata gatgtagatt taaataaagc tttaacaaaa gaaaaaaata
     1381 ttgatatgga catctataac ttggccagaa tattatcatt atagactggg ggttaaatca
     1441 tgagaaaaac gaaaattgta tgtactctgg gtcctgctgc aaatgataaa gagacattaa
     1501 agaacctggt agaggctggc atggatgtag cacgtttaaa tttttcccac ggagatcatg
     1561 aggaacatgg gaataggatt gaaatggtca aagaagtaga gaaggaaatt gataaaccga
     1621 tcggcataat acttgatact cagggtccag agatcagaac aggtaaaatg aagaatgata
     1681 aggtaacctt aaagaataac gctgaggtaa caatcaccac aaaagaagta gtaggaaccc
     1741 aggataaatt tcagattaaa tatgataact taccccagga tatcaataat gattccacta
     1801 tacttttaga tgatggttta ctggaattaa aagttaagga aattaagaag aaagaagaag
     1861 aaattgtctg tacagtaatc aacggagggg atttgggctc acataaagga gtaaatattc
     1921 ccggtattaa gaccacatta cccgcgatta ctgaaaagga taaaaaggat attatattcg
     1981 gtattaaaca taatattcac tttattgcag cctcttttat taggaaagcg tctgatgttt
     2041 tagagattag aaaagtcctg gaagaagaat gggcccagga tattaaactc atttcaaaaa
     2101 ttgaaagtga agaggcagtt aggaatatag atgaaattat tgaagtttcc gatggtataa
     2161 tggttgccag gggtgacctc ggggtggaaa tggccgccga gaaagtgcct attattcaaa
     2221 aaatgattat aaaaaaatgt aatgaagctg ccaaacctgt aattactgcc actcaattat
     2281 tagattcaat gattagaaat ccaaggccaa ctagagcaga agcttctgat gtagccaatg
     2341 ctatatttga tggtaccgat gctacaatgc tttcgggtga aactgctatt ggtagttttc
     2401 ccgttcaggc cgtaaaaact atggctaata ttgcaagaga aacggagtta tcagatacat
     2461 acgaagctca gctaagcagt tcaaaagcta taagggccga tactgtaaca gaagctataa
     2521 gtatcgggtc ctgtgaaatt gcttccggac tctatgctga cgcgataatt acagcaactg
     2581 gttccggttc tacggcaagg atggtttcta agtgccgttc aatgacccca attgtggctg
     2641 taacgcctaa tgatatggct ctccataatc taactttaag ttggggcgtt taccccacta
     2701 tggctgctga tagtacttca actgacgaga tgatggataa ttccataaat gttgctctgg
     2761 aaaatggttt agttgatgaa ggagatctgg tagtccttac ggccggagtt ccggtcggaa
     2821 caccgggtac caccaattta ataaaagtag atatagttgg tgacccaatt gtagaaggtc
     2881 agggtattgg tcgaaaaatt attcacgggc ctgtaaaaat tgttaatagt gccaatgaag
     2941 ctttagaaaa ggtggaagag ggcgatatat tagtagcccg ttctactaat aatcaatata
     3001 atgaagcaat tcaaaaatct gcgggtatta tagcagttga gggaggtgta acctctcatt
     3061 cagctgtagt tggtattcag tataataaac cggtagttgt agatgcagtc ggaataatag
     3121 atatattagt agacggtgag accgttacaa tagatagtgt gaggggacat atctacaaag
     3181 gtaaggtgaa cttaaagtaa aaattatgtt aaaatcataa attaatattg attttttaac
     3241 agttccccgt atataatcat acggggttaa cttttttagg aggtggtata aagatgagca
     3301 taaaaacaaa aaaagtaata aaaaattata taataattgc agccggagct attatgacag
     3361 ccctttcctt agtattattt acagtaccta ataagatcgc agctggagga gtaagtggta
     3421 ttgcaacaat actttaccat atgtttggat ttaatgttgg tatagttatt ttaattataa
     3481 atattccatt atttctagta gcagtatatg tattgggagg cagtataggt ataaaaaccc
     3541 tctggggtat aattgtaatg tctgtctcgg tagaagtttt gacacccctt ttaaatccac
     3601 taactaatga agtaatatta gccagtattt acggcggtgt tttaggggga gtaggtatgg
     3661 ggtttgtatt tagagggggc ggtacaaccg gtggtacaga tttgattgcc gccttaacaa
     3721 gttatttctt cccccatttt agtattggcc agggattatt tttaatagat gggattgtca
     3781 taaccttggc aggaatcgta tttgatgttg aattggccct atatgcagcg ataaccatct
     3841 ttgtcagtac taaactgatt gatataattc aggaaggttt taatatttca aaatcagtct
     3901 ttattatttc agatcggtcc caggaaatta aggataagat tttagataat atgcgccgcg
     3961 gtgtaacagc tcttagaggt tatgggggct atagcggtag agagaaagat gtacttttag
     4021 taactatcaa ccgggcggaa attactaagt tgaaaaactt aataagagat atagacgaag
     4081 aagcctttgt cattatgaat gaagcccatg aggtcttggg agaaggtttt aaaagaatta
     4141 atgaaagagc tataaatgaa tagataattt tacaactaaa gaccgctttt tataaattac
     4201 aataaatgat ataatataaa aaacaggata gtgaattttc taaaggaggt caaaagatgt
     4261 caatagttga ggaatttaac cttgaaaaga ttgaagaggg taaaacctat gaaatgtatg
     4321 aaggtagcta ttcatctgaa gaagagattg aaagaaaatt agaactagct ggatataaat
     4381 ttatttcgga agaacgcaga atatatgtta caaaagtata tgcagatgat atggaaaaaa
     4441 gaattgctgt acttgattcg gggaatataa ggatctggga ctttgattca gaagagtact
     4501 atgaaatgga attggaccac catagagaat aaatttaaaa ccataatatt ttattaaata
     4561 agggctagct ttttgttaat gtttaataat aaattttatg taaaattagt ttcataaata
     4621 tcacgtgaat gttataatta taataagcgg gtataactca aaggtagagt gcaaccttcc
     4681 caaggttgaa gttgcgagtt caagtctcgt tacccgctcc aattgtcagt cagtttactt
     4741 ttaagcttta ttattatata atattataat aacatgaaat atttgttgat ccttaattat
     4801 ggagggataa catgtcaaat gaatatgatt ttgttactgt aaaaattaaa ataaacaaat
     4861 tgaacaaaat gctccccgag atcactcata aacgagagaa agaattttta caaaagcata
     4921 tgccccgcta tctacatttt tatgattatt ttttaaagaa attgtttccg tttttatcag
     4981 actttaaaga agatatagcc tggaaagtga tacataatgg gatgagtttt tatgtatcaa
     5041 aagatagacc acaggacggc atgtttatgt cggttgcttt tcaagccaac aataaattga
     5101 agttctcttt ttactatgag gaagatgaag aggacagtga tttatcaaga gaatcacgtg
     5161 aaggaagaaa tgaagaccac atgtggtatt atgtggaaga tttggaattt gataaggaat
     5221 tagcgaagtt aattgtacag agttttaaag aaaaagttaa ataccttaat caaaaagata
     5281 atgaagaata aagaacaaac ctcctgcaaa aggaggtttt tgtctttata agagatttat
     5341 tcaatattta aaatttctac atctggattc attttatata tattatcttt tctttcaata
     5401 accacttctg ctttttcttt aagatcgggg tcttctttaa tacaactgat caaatcatag
     5461 ttaggattaa aggcaacggg attaccagtc atctgcagca tgctgtaatc accttgggta
     5521 tctccatagg agtaactttt ttctaaatca attttgtaat ctgagacaaa ttcattaagt
     5581 actttctctt tattattaga cttccacatt tttataacct ctccggtgaa atgaccattg
     5641 ttatctaata gatacttact accccgataa tcggttatat tatattttgc agccattttt
     5701 tccactaaaa actctggact acctgagata aaaaacacct tatgattttg tttcaaatga
     5761 taattaacta tacagcgagt ataacgataa acaatatctc cgcttaaatt aataacctgt
     5821 tcattgacaa agtctaggtc tgagattttc attcctttca gttcatttat atagatttca
     5881 gctaattctt ctagataatc ttcataatat ccgtgtcttt ttttccattc atcataggtt
     5941 tttttaactt ggctgtgcca gatgagagga tctaacactt catatttaat taatttctta
     6001 aaatgttgaa tcattaatga ccctcggtat aaagtaccat ctatatctaa aaatgctcct
     6061 atattcattt taccactcct atccttttag tactgctaat ggttttaaac gaactaccgg
     6121 tagggctagt tccgtttcta tcatggattc cactacatct tcaatatttt tatatacaag
     6181 tggtgattca tccaatagac tatcaccaga ggaagaggtt acttctactt cctttatagc
     6241 attttggtgt tcctgattag taatacttct ttttgcttcc cgacggccca tcacacgacc
     6301 ggaaccatgg gcaattgagt ttaaagattt ttctgtagat tcagaattag ctgacttaat
     6361 aatgtaactg gcggttccca tcgaaccagg taaaagagca aaagaatttg gttttaacct
     6421 ggttgcacct tttctgtgga ctaaatactc atcttcttta tcgttgattt tttgtttttc
     6481 gaatcttgcc atattatgtg ttaaatcata aaataaaggt agagattgat taggatataa
     6541 atcagcaaaa atatttctaa ctttgtgggt tattagctga cggttagcat aggcaaagtt
     6601 ggcagcacaa cccatcgccg ataaatagga acgtccttcg tgtgactcaa ttgggacaca
     6661 ggctaaattc ttgacaggaa atttaaaatt aaatttatgg tttgtattaa ctgcctggtc
     6721 tatatgatct ttggcaattt ggtgaccaaa gccccttgaa ccggtgtgaa tcattacagt
     6781 aatttggcct tcttttaaat ctgtatcatt ttcgaatact ttttcaacat attgcagctc
     6841 cacaaagtga ttaccagaac ccagactgcc taactgggta acacctcgct gaatagcttt
     6901 ggaagaaa
//
LOCUS       DATGCJ010000182         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_339674, whole genome shotgun sequence.
ACCESSION   DATGCJ010000182 DATGCJ010000000
VERSION     DATGCJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339674"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            575..2101
                     /locus_tag="VKN64_08545"
     CDS             575..2101
                     /locus_tag="VKN64_08545"
                     /inference="COORDINATES: protein motif:HMM:NF021349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA60325.1"
                     /translation="MKYLPLGNQDFKELIENNYIYVDKTKWIYDLLKVPKGIYFLSRP
                     RRFGKTLTISTLHHLFKGNEELFKNTFIYDKWNFQERPVIRLNMNEVNSIKNTEVFED
                     ELLRIIKNQAEINNVTLSESLYSAAFQELIANLDNPAVLIDEYDKPILSNIHKPEYVD
                     QIREILKEFYGAIKTKESNLSFIFITGISKFSKMSIFSELNNLTDLTLRKDYADMLGL
                     TDDEIDTYFREYIEKVKKEQNMTESDFRNRLKDYYNGFSFDGEHFLYNPYSILNFFAT
                     SEFRNYWFESGTPSHLIELIKSEMLKSTDFVNKRVGIDFTSRKPIESTSPESFLFQAG
                     YLTIKESRERVYVLDYPNNEVRSSFNSLFLDSIYDIENESAIIDDIWIGFENNDFAMV
                     FEQFQQTFSQIPYPLYKNEQENIYHIVLLTLLWATGIKTTAEDINNLGRSDIVIEFDN
                     DVYILELKRDSADKALQQIKDKEYYKKYTGKNRSISLVGIEIDDDKRNLKDYKLEQIS
                     "
BASE COUNT          897 a          445 c          450 g          746 t
ORIGIN      
        1 taagattaaa accagagaga aagaatacaa acaaggtgag ataacagaag caaaactcca
       61 gcagtctgta aatagtatta ttgctcattt gagtaattac aatacttata atcttagaaa
      121 agatatcttc aaaggattat ggtaaaaaag ggtatctacc tgcgaggttc gaaccgagtc
      181 aaacgtggcg gtagttataa caacaatgcg aggaactgtc ggacttcaaa tcgtaacaac
      241 aacaacccgt ccaacagcaa caacaacctc ggtttccgcc tcacaatact ggcaattgcg
      301 agaactttgc gtccacggat tcaaagtcag tgccctgcct gtctaaggtg gatatccagc
      361 acaggaaata tcctgtcaaa aatggtatgg cttcccgggg attggtaggt ttactctcga
      421 agatgcccgg gaagagctct gctttgcgat aaaattatcc aaatcaaaca ctctcctatg
      481 ttttttaatc atcaatcttc aatcaagaaa cttaaccctt taatttaacc tataaaataa
      541 tgaaattatg atacataaat taggaggtaa taaaatgaaa tatttaccct taggaaatca
      601 agattttaag gaactaattg aaaataacta tatatatgta gataaaacta aatggatcta
      661 tgatctacta aaagtaccta aaggtatcta cttcctctcc cgcccccgtc gatttggtaa
      721 aactctgaca atttcaactc tccatcacct tttcaaaggg aatgaagaac tatttaaaaa
      781 cacttttatc tatgataaat ggaactttca ggaacggcct gttattcgtt tgaatatgaa
      841 tgaagttaat tcaattaaaa acacagaggt ttttgaagat gaattactaa gaattatcaa
      901 gaaccaggcg gaaattaata atgttacctt atcagagtct ttgtattccg cagcttttca
      961 ggaattaata gcaaacttag acaatcctgc cgtcttaata gatgaatatg ataaacctat
     1021 cttgagcaat attcataaac ctgaatatgt tgatcagatc agagagatct taaaagaatt
     1081 ctatggtgcg attaagacta aggaaagcaa tctaagtttt atctttatta caggcatatc
     1141 caaattctcc aagatgagca ttttctcaga actcaacaat ttgactgatc taaccctaag
     1201 aaaagattat gctgatatgt taggcttaac agatgatgaa attgatacat actttagaga
     1261 atatatagaa aaagtgaaga aagaacagaa catgacagag agtgactttc ggaataggtt
     1321 gaaagattat tataatggtt tttcctttga tggtgagcat tttttataca atccttattc
     1381 aattctcaac tttttcgcca cctccgaatt cagaaactac tggtttgaat cgggaactcc
     1441 ttctcattta attgaactta tcaaaagtga aatgctcaag tccaccgact ttgtcaataa
     1501 aagagtaggt attgatttta catcacgaaa accgatagag agcacttctc ctgagagttt
     1561 tctattccag gctggatatt tgactattaa ggagagtaga gaaagagttt atgtactgga
     1621 ttatccaaat aatgaagtcc gatcatcctt caactctcta tttctagatt cgatttatga
     1681 cattgaaaat gaatcagcaa taattgatga tatctggatt ggatttgaaa ataatgattt
     1741 tgcaatggta tttgaacaat ttcagcaaac tttcagtcag ataccatatc cactctacaa
     1801 aaacgagcag gaaaatatct atcacatagt tctgctaaca ctactatggg caacgggaat
     1861 taaaacaact gctgaagata taaataatct gggacgcagt gacattgtca ttgaatttga
     1921 taatgatgtc tatattctgg agctcaagcg tgattcagca gataaggcct tacaacaaat
     1981 taaagataaa gaatattata aaaaatacac tggtaaaaat agaagcatat ccttagtagg
     2041 cattgagata gatgatgata aaaggaattt gaaggattat aaattagagc agatatcata
     2101 atcctacctt ttcaatcata aatcatcaat ccaacccaac cacctctccc ccaccacctt
     2161 accccatggg gatacgcatt tcacccagat ggggatacgg atggggatac ggagccgatc
     2221 tcgacgaggc tatttacggt tatttggggt tatttacgga attgaagccc aaatatcggc
     2281 aaaatagccc tgattttgtg gtttcagggc tatgattcta aaatgtaagt tattgttttt
     2341 atttagagta ccccgagaag gactcgaacc ttcaaccgtc ggcttaagag cactacgaca
     2401 agagtcatat atgtttgcat attcctgaat agcagtaagt tatagatgtt atttcaagta
     2461 atagtgatac ggtatcggga tacggatgat ttatgtattt ataatcagaa ctgtttaaag
     2521 agcaatcacc cattatct
//
LOCUS       DATGCJ010000183        15399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_605331, whole genome shotgun sequence.
ACCESSION   DATGCJ010000183 DATGCJ010000000
VERSION     DATGCJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605331"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2755
                     /locus_tag="VKN64_08550"
     CDS             <1..2755
                     /locus_tag="VKN64_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMA60326.1"
                     /translation="ALDMREATDMVSGILPDDAGEPLIVQFDPSMIPVLQLGVYSGQN
                     LADLKTNVEDNVIPRLERLPGVASVNLTGGQDREILIELDRNKMENYGVDFNSVTQNL
                     LLENFNLSGGQVDRGNIEYLVRVTGKFENIDQIKEVLIPTGSRQGFVKLSDIANVKDT
                     FKEMQSITRVNEQPSIGLTIQKQTDANTVAVANRVRDEIDEIKKDQPNLNLVPIADQA
                     EYIEDSINTVYRNALLGGLLAVIVLLLFLRNFRSTIIISLAIPISVVATFLLLYFGGL
                     TINIISLGGLALGIGMLVDNSIVVLENIFRYKQYGLDRVEAASRGSEEVGMAITASTF
                     TTIVVFLPVIFVQGLTAQIFRELALTVSFSLFASLIVALTLIPMLSSKLLKLSKKQMK
                     LGKDTTLGYVKRNYQSSLRWFLHHRWLIIVIIVLLGVGAGFLAVDLGTEFLPQFDQGQ
                     FTVEYSLPVGTVIEETDDVAVQLEDTIDQIPEVETIFTNIGVSNMMSGSTSSETGSFT
                     VILKDLAERDRSTSEVMEELRQKVKIPGADIGIESQSGFFGPTGGAPINIKVVGQNLE
                     ELERLSGLVMEQMRQVEGVREVEDSFEEGRPEFVIQIDRALAARLGLRVRSVANTVKT
                     AITGSVATQFEVGGDQYDVRVTLTEEDKRNIEQIKNIMLPSPTGAKVPLSRVADFELS
                     KGPKQILRINQERYAEITASLFQTDLGTAVNAIQERVNENVEFPDGYNIRYGGQFQDL
                     QQSFNDLFYAFLLAIVLVYMVMASQFESLVHPLVIMFTVPLAVVGVIFGLYITNTTIS
                     VPALIGIITLTGIVVNNAIVLVDYINRLRDEGKTKIESILEAGPIRLRPIMMTALTTV
                     LALVPLGLGIGEGSELEQPLAIVVISGLLFSTFLTLYVIPVFYSLFTDISDKIKEKLR
                     KVV"
     gene            2947..3168
                     /gene="secG"
                     /locus_tag="VKN64_08555"
     CDS             2947..3168
                     /gene="secG"
                     /locus_tag="VKN64_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMA60327.1"
                     /translation="MFALKIFHLIVAIAVIVGVLLQSGKSAGMSGVIDGGASQFFGKK
                     NKGLDEKLSKFTTVAAIVFMLTSLIIAFI"
     gene            3202..5154
                     /locus_tag="VKN64_08560"
     CDS             3202..5154
                     /locus_tag="VKN64_08560"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMA60328.1"
                     /translation="MNFITPAAGVIALLFALYMASKVKKADVGTDRMKELSRSINEGA
                     MAFLGREYRILVFFVLIVAVIMSFTGNLGWQTAVSFILGAIFSGLAGFIGMQIATQAN
                     ARTTNAAQSSLNKALQIAFSGGSVMGMSVVGLGTLGLGILYIFFTKNIDYIRGFAFGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGSIVAAMTLGTVMSVNHVVTPLLLAALGIVSAIIGSQFIKLKEGGNPAKA
                     LEKGTIGSAILMVIGAFFLIRYMIGDIGVFYAVVAGLVAGILIGRITEYYTSEHFGPV
                     KYIAEQSESGTATNIISGLSVGMRSTLFPILVIAVVIFIAYHFAGLYGIAMAAVGMLA
                     TTGVTLSIDAYGPIADNAGGIAEMAELPAEVRERTDKLDSVGNTTAAIGKGFAIGSAA
                     LTALSLFAAFSSAVNLDGISLTNPNVIIGLFIGAMLPFLFSSLTMDAVGKAAFQMIAE
                     VRRQFKEIPGIMEGEGTPDYKKCVDISTAAALKQMVVPGLLAVIVPVVVGLWNVHALG
                     GLLAGALSSGVLLAIMMANAGGAWDNAKKYIEAGNFGGKGTDTHAASVVGDTVGDPFK
                     DTAGPALNILIKLMTIVSLVFAPLFI"
     gene            5261..5665
                     /locus_tag="VKN64_08565"
     CDS             5261..5665
                     /locus_tag="VKN64_08565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMA60329.1"
                     /translation="MDLGNDNMCFACGEDNPISLGLKFTKIGDKKVKAAFTPEEVHQG
                     YDGIMHGGLITTLLDEAMAKVLSLNNIPALTARMNIRFKHPLPIGTELVVTAEIIKNK
                     TGLYFTEAEVRSTEGKLYASAEAKFMQVKEAK"
     gene            5667..6218
                     /locus_tag="VKN64_08570"
     CDS             5667..6218
                     /locus_tag="VKN64_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HMA60330.1"
                     /translation="MEREEAYQLVKENISQDNLVKHCLAVEAVMRRLAEYFDKDKEKW
                     GLTGLLHDIDYEETADDPEQHSIVGAEMLKEMGMDDDIVYAVKVHNHEHGFERKSLMD
                     KALYASDPLTGLIVASALIHPEKSLEAVDTEFVMNRYSESSFAKGADRDVIASCKEMD
                     LSLEEFIGLALEGMKSISDDLGL"
     gene            6258..7253
                     /locus_tag="VKN64_08575"
     CDS             6258..7253
                     /locus_tag="VKN64_08575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMA60331.1"
                     /translation="MILVTGATGHLGNVLVRKLLKETKENIRVLVLPGEDYSILEDFD
                     IEIIVGDICDLNSLLPVFEGVDLVYHLAGRVSIKDFDPALKAINLDGTKNVIKACLDN
                     QVKRLLYVSTVHVFVPEVISEITEENPIVIQELRGEYSKTKAMATLEVMRASHWDLDA
                     VIVYPSGIIGPYDYRISPMSRLIIDHIKGKFMCSVEGSYDFVDVRDVADGIIKAAQHG
                     KKGEGYILSGKNISINEIFKIIDGLKKPFFKTFNLPLWMAKIGIPFSHTYNKIVGCEN
                     LLTRYALYTLNTHTKFSNKKAKVNLGFKPRPIKITLADTIEWLEEHGLIKEEVYR"
     gene            7250..9367
                     /gene="rnr"
                     /locus_tag="VKN64_08580"
     CDS             7250..9367
                     /gene="rnr"
                     /locus_tag="VKN64_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMA60332.1"
                     /translation="MSARSKLLDFLEEAYRPMTKEELMEEFEISSQQKQHFEDLLKQL
                     QKDGLIYKSKQDKYGLPEKFDLVAGRIDKNPRGFGFLIPEEPGHEDIFINPTNLNGAM
                     HNDKVYVKKVPGSSGKKAEGEVVEIIERANTQIVGRFEKVKNYGFVVADNKRISYDIF
                     IPGAHINDAKNNQKVVAEITEWPKKNRNPEGKITEILGYQDEKGVDIEAIIRQLELPG
                     DFPGRVKNELGRIPETIPAEEIEKRRDLRDLPMVTIDGEDAKDFDDAVSIEKLSDNKV
                     RLGVHIADVTYYVQEDTNLDEEALHRGTSIYLVDRVIPMLPQKLSNNLCSLRPNEDRL
                     SMSVLMDIKLNPLSLESYDITPSIINSNHRMTYNEVQSILVHENRELRELYDDFVDQL
                     ELMNKLRDKLRTKRFNEGSIDFERTEVKVILDDTGKPIDIQEERHGVAEQLIEEFMIM
                     ANKVVAEDMYWRDVPFIYRIHEPPDQERLKEFNEFIHNFGYHVKGIESEVHSSALQDV
                     LNQVEDEPEENLINTVLLRTMKKAIYSQHNIGHYGLGLDYYTHFTSPIRRYPDLMIHR
                     IIKEVLKHGKLSNNRFNELEERIPSIADHSSLQERRAMEAERDSVELKKIEYMQDKVG
                     EEFEGLVSGITSFGFFVELENTVEGLVHVEDLKDDYYHIREDIHALVGERTNKRYRLG
                     DEVKVKVIRVDPEEREIDFILVD"
     gene            9384..9857
                     /gene="smpB"
                     /locus_tag="VKN64_08585"
     CDS             9384..9857
                     /gene="smpB"
                     /locus_tag="VKN64_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMA60333.1"
                     /translation="MSDDIKVIARNKKAKHDYHIEETYEAGIILKGTEIKSIRNRNVN
                     LKDSFAMVENGEVFLYNMHISPYKQGNRYNHDPERKRKLLLHKNEIRKLIGYTKRKGY
                     TLIALSVYLKNNLAKVELALAKGKNVRDKRRDIAKKTAERDIERAYRRRLKGKDY"
     gene            9918..11390
                     /locus_tag="VKN64_08590"
     CDS             9918..11390
                     /locus_tag="VKN64_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HMA60334.1"
                     /translation="MSETEYYSENLRPQIHFSPPKNWMNDPNGLVYYKGEYHLFYQHN
                     PTETRWGNIHWGHAVSKDLLHWEHLDIALKPDEYGLMFSGSAVVDKDDTSGFFDGEDG
                     LVAIYTTALPQKNKDFYLQQQCIAYSKDKGRTWHKYEGNPVLENENWQNFRDPKVFWH
                     DETDSWVMVVTQGKKVNFYRSDDLKNWDFVSDFSCNAFYFEGVWECPDLIKLPVENED
                     EAKWLLQVGVQDNSRAGGSGVQYFVGEFDGYEFTNLNPDQKTFWVDYGQDFYAVQSWF
                     NLPTEQNREIWIAWMNNLAYSGAIPTNDWRGVLSVPREIDLKKFNNEYYLKQKPIKEL
                     DNLKEREDSILEHDLVNDFTCKLDFDPFEVNFQATIDSKDKLNVNLIRGNNLKINLLY
                     DRKNREVVLRRSFRIDKKMGQFFNDTHTSLIEEDKEKVDIQLIMDRSTIEIFINGGEI
                     VFSDLIFPEEDGSTIFSIITDSKQGKVYDLKINKLESIWS"
     gene            11410..11949
                     /locus_tag="VKN64_08595"
     CDS             11410..11949
                     /locus_tag="VKN64_08595"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMA60335.1"
                     /translation="MKIYTRSGDKGNTSLFDNSRVPKDSIRVESYGTLDELNSQLGMA
                     RSMLKNKEIAGTIKKIQRQLFNVAGELATPNSEDFPERVKPEDIEELEGIIDNLLDKM
                     NKEQLSKFIIPGSNRCSAALHVARTICRRAERRIITLAREEEIREELLKYVNRLSDVI
                     YSLARYVESELDYVDFKKE"
     gene            11968..13023
                     /locus_tag="VKN64_08600"
     CDS             11968..13023
                     /locus_tag="VKN64_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HMA60336.1"
                     /translation="MRFDERDIMFARINYEPGTKIYEEYYEKNPEKKEFDDFLRSLPG
                     FMVEEAFSFDPLNSGLVNGTYNFLSDLKKQAEGEASENTYQVEQDEISKKLKQIAKYY
                     GADLVGITEMKDEFYYNHRGREKAHHGKKVTNKHKYGIVFAVELEKDMVNRAPRLGQS
                     LETSKGYVKAGIIGMILSYYLRELGYEARNHMDGNYLLMAPFTAEAAGLGEIGRMGIL
                     VTKEYGPRIRLGVVTTDLKLNTDQRDPFGMKEFCKICNKCADNCPSDAIDSGSPGQLN
                     GEARWKTEVEKCFKVWQMSGTDCGICIASCPFSQNLDFDKINKMKDNPKVMRDILRKY
                     NQEHEERPYNESEHEWL"
     gene            13060..14490
                     /locus_tag="VKN64_08605"
     CDS             13060..14490
                     /locus_tag="VKN64_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60337.1"
                     /translation="MKKPGYIFLIFLIILPVFIFNVQAAEINFIKKDKLITELNLDRN
                     IEIIDKRFIDLTGDGIKEQVFLIGQRPAGPDSLFYAPLKVIVYDIYKKEFYVNSYDNF
                     NGYEPQMAFYDFTGDQIKDIFIEANTGGSGGIYKHLIATVLDNDLKIIFNESHNKPLH
                     MAGNYEDNFKTILRINEIDKNLRLDLSFNRNKYVQEGIYNSNGTLQREIQPYTYPFGQ
                     LKPVDYDLDQTMELKGIQRIVGAYGADTIGHLETILNYDGSWEILEVQISTYLKKYDP
                     SRSGSNGEVSYKIKQNAITSGIKEIYYPQITDLPKDVNSVYINKQLHDIVKPFLAESN
                     EMSIDYDFTVKNKKLFSVKFSGYQKHEGGQYEILKSFNYDIKNNRRLTLSNILKDETH
                     DKDKVNQIIKDNIEDKDLRDTFPGLSDWMGFYLTNSDLVLYYLRDDFETKFNKIRVPL
                     RKLRQYLSFEVDIQRDETEIFIIEVD"
     gene            14492..>15399
                     /locus_tag="VKN64_08610"
     CDS             14492..>15399
                     /locus_tag="VKN64_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMA60338.1"
                     /translation="MGNNKIDKIKEYIDKNQKLNLEEVLSEFDENSTPIIDKREDNYL
                     LSFLYEENDLKNISVVGSFPGFDLRNQKLFKIGDSKIWTQSFEVDQPVNFTYGFIKNY
                     DEDDRELDIRSEMIKDPFNEEEITIYQGEDQILNLSSYDMVYDKEVDRYFNKKYEGEP
                     LIEENDIKSEIYEDERRYWTLESTQDKDYEGIFLCTDGFTYINDNQLLKVLDNLRKEN
                     LIPNFLFVFVENKDRGVELAADQDFKNFLNDELLGEIKEEYNIKLKEHDNIICGKSLG
                     GLTAFYTAYDSELFNSIISHSGTFIWG"
BASE COUNT         5117 a         2455 c         3417 g         4410 t
ORIGIN      
        1 cgctttagat atgcgggaag ctacagatat ggtctccggt attttacccg atgatgcagg
       61 tgaaccgttg atcgtccagt ttgacccctc catgatcccg gttttacagc tcggtgttta
      121 cagtggtcaa aacctggcag accttaaaac aaatgttgaa gataatgtaa taccacgtct
      181 ggaaaggctg cccggggtag cttcggttaa tctaacaggt ggacaggacc gcgaaatatt
      241 aatagaacta gaccgtaata agatggaaaa ttacggggtg gactttaatt ccgtcactca
      301 aaatttattg ctggaaaact ttaacctttc tggtggccag gtagaccgtg gtaatattga
      361 atacctggtc cgggtcacag gtaaatttga aaatatcgat cagattaaag aggttttaat
      421 ccctaccggt tcgcgtcagg gctttgttaa actcagtgat atagctaatg taaaggacac
      481 ctttaaagaa atgcagagca ttacgcgtgt aaatgagcag cccagtattg gcctaacaat
      541 tcaaaaacag accgatgcta atacagtggc tgtggccaat cgcgtcaggg atgaaataga
      601 tgagatcaaa aaagatcaac ctaatcttaa tctggttccc attgccgacc aggcagaata
      661 tatagaggat tcgatcaata cagtttatag gaatgcccta ttaggtggtc ttttagcagt
      721 aatagtctta ttattattcc tgcgtaattt cagaagtacc attattattt cactggccat
      781 tccaatttcg gtagtagcca ccttcctctt attatatttt ggaggactta ctattaacat
      841 catctccctg gggggacttg ccctcgggat cgggatgctg gttgataact ccattgtggt
      901 tttagaaaat atattccggt ataaacagta tggtttagac cgggttgaag cggccagccg
      961 cggtagtgaa gaggtgggaa tggcgattac tgcctctact tttactacca ttgtagtatt
     1021 tttaccagta atatttgtac agggactaac agctcaaata ttcagagaac ttgcattgac
     1081 cgttagtttt tctctctttg cctctttaat tgtggcgtta acattaatcc cgatgctttc
     1141 ctctaagtta ttaaagctga gtaaaaaaca gatgaagttg ggtaaagata ccacattagg
     1201 atatgtaaaa agaaattacc aaagttcttt aaggtggttt ttgcaccacc gctggctaat
     1261 aattgtcata attgttcttt taggagttgg ggccggtttt ctagcagttg atttaggaac
     1321 cgaattctta cctcaatttg accagggaca gtttacagta gaatatagtt taccggtcgg
     1381 aactgtcata gaagaaaccg atgatgtggc agttcaactt gaagatacga ttgatcagat
     1441 cccggaagtt gaaacgatct ttaccaatat tggggttagt aatatgatga gtggttccac
     1501 ttcctctgaa acaggttcct ttacagttat cttaaaggat ttggctgaaa gagaccgttc
     1561 aaccagtgaa gtaatggaag aattaagaca aaaagtaaag ataccaggtg cagatatagg
     1621 tattgaatcc caaagtggat tctttggacc gaccggtggt gcaccaatta atatcaaagt
     1681 ggttggtcag aatttagaag aattagaaag actatcaggg ttagttatgg aacagatgag
     1741 acaggtcgaa ggtgtaagag aggttgaaga ttcctttgaa gaaggaagac cggaatttgt
     1801 aatccaaatt gatagagcat tggctgcccg tttaggtctc agggtaagaa gtgtagccaa
     1861 taccgtaaag accgctataa cgggaagtgt agctacccag tttgaagttg gaggtgacca
     1921 gtatgatgta cgcgtaaccc tgactgaaga agataaaaga aacattgaac agatcaaaaa
     1981 tattatgctg ccttccccga ccggagcgaa agtgccttta tctagagtag ctgactttga
     2041 gttatcaaaa gggcccaaac agatcttgcg gattaatcag gagcgttatg cagaaattac
     2101 tgcttcctta ttccagactg acttgggtac cgctgttaat gcaatacaag aaagagtcaa
     2161 tgaaaatgtg gaatttccgg acggatataa tatcaggtac ggaggacagt tccaggattt
     2221 acagcagtcc tttaacgatc tattttatgc atttttactg gctattgtgt tagtctatat
     2281 ggtaatggcc tcccagtttg aatcactggt tcatcccctg gtaattatgt ttacagtgcc
     2341 cctggctgtg gtcggtgtta tcttcggttt atatattact aatacgacga tctcagtacc
     2401 ggccttaatt ggtataatta ccctgaccgg tatagttgta aacaatgcga ttgtacttgt
     2461 tgattatata aataggttaa gagatgaagg caaaacgaag atagaatcaa ttttggaagc
     2521 aggaccgatc aggttgagac caattatgat gactgcttta actactgtct tagcgctagt
     2581 acctctagga ttaggaattg gagaaggttc agaattagaa cagccgttgg ccattgtggt
     2641 aattagtgga cttctattct ctacattctt gaccctatat gtaattccgg tattttactc
     2701 actgtttaca gatatcagtg ataaaattaa ggaaaagtta cgaaaggtag tttaaataag
     2761 ctaaatattt agtaaataat aacaaaaagg gggttgattt aatacaaaca actcccctct
     2821 ttgtttaact cttacaaaaa atccattaaa tctacttaat atattgtatt tttaccatca
     2881 ttatgttaaa atgtaaaagt gctgaggaat gaatagcgaa ataataattg ttgggggtga
     2941 cctatagtgt ttgcactaaa aatctttcac ttaatagttg cgatagcagt aatagtaggt
     3001 gtcttacttc aatcaggaaa aagtgctggt atgtccggtg ttattgacgg tggagccagt
     3061 caatttttcg gtaaaaagaa taaaggtttg gatgaaaaat taagtaagtt tacaacagta
     3121 gctgctatag tgtttatgtt aacttcatta atcattgcat ttatctaaaa aatcaaatat
     3181 agacatgggg gtgttcgaaa gatgaatttt ataactcctg ctgcaggagt gattgcacta
     3241 ttattcgcat tatatatggc ctcaaaagta aagaaagctg atgtgggaac cgatagaatg
     3301 aaagaactat caagatccat taacgaagga gcaatggctt ttttaggccg tgaatataga
     3361 atattagtgt tttttgtttt aattgtagca gtaataatga gttttacagg taatttaggc
     3421 tggcagaccg ctgtttcatt tatattagga gcaatttttt ctggattggc tggatttatc
     3481 gggatgcaga ttgctaccca ggccaatgcc agaaccacca atgctgctca gagcagctta
     3541 aataaggcct tgcagattgc attttctggt ggatcagtaa tgggaatgtc agttgtaggt
     3601 ttaggtacac ttggactagg aattttatac atctttttta ctaaaaatat tgattatatt
     3661 agaggttttg cttttggagc cagttctata gctctttttg cacgtgtagg cggtggaatt
     3721 tataccaagg ctgccgatgt aggagctgat ctagtaggta aggtcgaagc tggtattccg
     3781 gaagatgatc ccagaaaccc ggctgtaatt gctgataatg taggagacaa tgtcggcgat
     3841 gtagctggaa tgggagcaga cctttttgaa tcatatgtcg gttctattgt cgcagcaatg
     3901 acattaggaa ccgtaatgag tgttaaccat gtggttacac cattattact ggcagcatta
     3961 ggaatcgtgt ctgccattat aggtagtcag tttattaaat taaaggaagg cggtaaccct
     4021 gctaaggcac tggaaaaagg tacaatcggt agtgctatat taatggttat cggagctttc
     4081 tttttaatta gatatatgat tggagatatc ggagtattct atgctgtagt agccggtctt
     4141 gttgccggaa ttttaattgg caggattaca gaatactata catccgaaca ttttgggccc
     4201 gttaaatata ttgcagaaca gtctgaatct ggcactgcaa ctaatattat cagcggtcta
     4261 tcagtaggga tgagaagtac cttattcccg atcttagtaa ttgctgttgt tatctttatt
     4321 gcttatcatt ttgcaggact ttacggtatt gcaatggctg ccgtcggtat gttagctaca
     4381 accggtgtta ctctctcaat tgatgcctat ggaccaattg ctgataacgc tggtggaatt
     4441 gccgagatgg cagaattacc agcagaagtt cgcgaaagaa cagataaatt agattcagtc
     4501 ggaaatacaa cagctgctat tggtaagggt tttgcaatcg gttcagctgc cttaacagct
     4561 ttatcattat ttgcagcttt ttcttcagcc gtaaatctag atggaattag tcttactaat
     4621 cctaatgtta ttattgggct attcattggt gctatgttac cattcctctt ttcatcacta
     4681 acaatggatg ctgtaggaaa agcagccttc cagatgatcg ctgaagtaag aagacagttt
     4741 aaagaaattc ccggtattat ggagggagaa gggacacccg attataagaa gtgtgttgat
     4801 atctcaacag cagctgcttt aaaacagatg gtagtacccg gacttctcgc cgttattgta
     4861 cccgttgtag ttggcttatg gaacgtacat gctctaggcg gccttttggc cggtgcccta
     4921 agttcgggcg ttctcttagc gattatgatg gcaaatgcag gcggagcctg ggacaatgct
     4981 aaaaaatata tcgaagcagg caatttcggc ggtaaaggta ccgatactca tgccgcttcc
     5041 gttgttggag atacagttgg tgatccgttc aaggatacag ctggtccagc acttaatatt
     5101 ttaattaagt taatgacaat agtttcatta gtattcgcac cattatttat ctaagagatt
     5161 aaaataaaag aacttctaat attaagatat aaaaagggca attatcttct gtaacagggg
     5221 gtttttgcct tttttttaat tttacccaag aggtgatata atggatttag gaaacgataa
     5281 tatgtgtttt gcctgtggag aggataatcc aatctctctc ggcttgaagt ttacaaagat
     5341 aggagataaa aaagtcaagg cagcttttac cccagaagaa gttcaccaag gttatgacgg
     5401 aattatgcac ggcggcttga tcaccaccct attagatgaa gcaatggcta aggtattatc
     5461 attaaataat atcccggcct taaccgcccg catgaatatt agatttaagc atcccctgcc
     5521 cattgggacg gagttagtag tcacagctga aataatcaag aataaaacag gattatactt
     5581 tactgaggca gaagtaagaa gtacagaagg taaattatat gcaagtgcag aagcaaaatt
     5641 tatgcaggta aaggaggcaa aataaaatgg aaagagaaga agcatatcaa ttagtaaaag
     5701 aaaacataag ccaggataat ttagtaaaac actgtctggc tgtagaagcc gtaatgagaa
     5761 ggttggcgga gtattttgat aaggataaag agaaatgggg attaaccgga ttactgcatg
     5821 atatcgacta tgaagaaaca gccgatgatc ccgagcagca cagtattgta ggagcagaga
     5881 tgttaaaaga gatgggtatg gatgatgata ttgtctatgc tgttaaggtc cataaccatg
     5941 aacacggttt tgaaaggaaa tcactcatgg ataaggccct ttatgctagc gaccccctaa
     6001 caggattaat cgtagcgtcc gctttgatcc accccgaaaa aagcttagaa gcggttgaca
     6061 ccgaatttgt tatgaacagg tattccgaga gttcctttgc caagggggct gaccgagacg
     6121 tgatagcttc ctgtaaagag atggatttaa gtttagaaga gtttattgga ctcgctttag
     6181 aaggaatgaa gtctatttct gatgatttag gcctctagtt gtattggact ccggtgaatt
     6241 gaagggaagc gattactatg attttagtaa ccggggcaac aggacattta ggcaacgttt
     6301 tagtaagaaa acttttaaaa gaaaccaaag agaatataag ggtattagta ctgccgggtg
     6361 aagattacag tatcttagaa gatttcgata ttgaaatcat agtaggggat atctgtgatt
     6421 taaattcctt gttacctgtt tttgaagggg ttgatctggt ctatcacctg gcaggcagag
     6481 tatctataaa agattttgac cctgccctga aagcaattaa tctggatggt accaagaatg
     6541 taattaaagc ctgtttggat aaccaggtta aaagacttct ttatgtaagt accgtccatg
     6601 tatttgtacc agaggtgatc agtgagataa ccgaagaaaa ccccattgtt attcaggaat
     6661 taagaggcga atattctaaa acaaaggcca tggctaccct ggaagttatg agggcttccc
     6721 attgggactt agatgctgta attgtctatc ccagcggtat aataggtcct tatgattatc
     6781 ggatttctcc catgagccgc ttaataatag atcacataaa aggtaagttt atgtgttcag
     6841 tagaaggttc ttatgatttt gttgatgtaa gagatgtagc tgatggaata attaaagctg
     6901 ctcagcacgg caaaaaaggg gaaggctata tcctgtccgg taagaatata tcgatcaatg
     6961 aaattttcaa aataatagat ggtttaaaaa aaccattttt taaaactttt aatctgccct
     7021 tgtggatggc taaaatcggt atcccatttt ctcataccta taataagata gtaggctgtg
     7081 agaacttatt aacccgctat gccctctata cattaaatac tcatactaag ttttccaata
     7141 aaaaagcaaa ggttaatctg ggctttaaac ctagaccgat aaagatcaca ctggcagata
     7201 ctatagaatg gttagaagag catggcttaa ttaaagagga ggtctatcga tgagtgcacg
     7261 gtcaaaactg cttgattttt tagaggaagc atatagacca atgacaaaag aagaattaat
     7321 ggaagagttt gagatttcat ctcaacaaaa acagcatttc gaagacttat taaaacaact
     7381 gcagaaagat ggtttaatct acaaaagcaa acaggacaag tacggtctgc ccgaaaagtt
     7441 tgatctggta gcgggccgga tagataaaaa cccgcgtggt tttggatttt taattccaga
     7501 agaaccgggc catgaagata tttttatcaa ccctaccaat ctcaatggag ccatgcacaa
     7561 tgataaggtc tatgtcaaga aggtgccggg tagttcaggt aaaaaggccg aaggtgaagt
     7621 tgttgaaatc attgaaaggg ccaataccca gattgtcggt cggtttgaaa aggttaaaaa
     7681 ctacggcttt gtagttgctg ataataagag gataagttat gatatattta ttccgggtgc
     7741 ccatataaat gatgctaaaa acaaccagaa agtagtagct gagattacgg aatggcctaa
     7801 gaaaaataga aatccagaag gtaagataac tgaaattttg ggttatcagg atgaaaaggg
     7861 cgtcgatatc gaggccatta tccgccagtt agaactaccg ggtgattttc ccgggcgagt
     7921 taagaatgaa ctgggcagaa ttccagagac cattccggct gaagagattg aaaaacgcag
     7981 agatcttaga gatcttccca tggttactat tgacggggaa gatgccaagg actttgatga
     8041 tgcagtttct atcgaaaaat tatctgataa taaggtgcgg ttgggagtgc atatcgcaga
     8101 tgtaacttac tatgttcagg aggatacgaa cctggatgaa gaagccttgc atcggggaac
     8161 cagtatctac ctggtagata gggtaattcc gatgttaccg cagaagttat caaataatct
     8221 ctgcagcctg cgtccgaatg aagacaggct ttcgatgtcg gtcttaatgg atatcaagtt
     8281 aaatccctta tctttagaga gttatgatat aacaccttcc attataaatt caaaccatag
     8341 aatgacatat aatgaagtgc agagtatttt ggttcatgaa aatagggaac tgagagaact
     8401 ttatgacgac ttcgttgacc aattagaact tatgaataaa ttaagagata aattaagaac
     8461 caagagattt aatgagggaa gtatagattt tgaaagaact gaagttaaag ttattttaga
     8521 tgacacgggt aaaccgattg atatccagga ggaaagacac ggtgtggccg aacagttaat
     8581 tgaagaattt atgatcatgg ctaataaagt tgtagcagaa gatatgtact ggcgggatgt
     8641 accctttatc tataggatcc atgaaccacc cgatcaagaa aggcttaagg agtttaatga
     8701 gttcatccat aacttcggct accatgttaa gggaattgaa agtgaggttc attccagtgc
     8761 ccttcaggat gtcttaaatc aggtagaaga tgaaccagaa gaaaatttaa tcaataccgt
     8821 cttgctcagg accatgaaaa aagcgatcta ttcccagcat aatatcggac actatggtct
     8881 gggcttagat tactatactc actttacttc cccgatcaga aggtatcccg acttgatgat
     8941 ccataggatt ataaaggaag ttttaaaaca tggtaagtta tcaaataata gatttaatga
     9001 attagaagaa agaatcccca gtatagctga ccacagttcc ctacaggaga gaagggctat
     9061 ggaagctgaa cgggattcag ttgaactgaa aaagattgaa tatatgcagg ataaggtcgg
     9121 tgaagaattt gaaggtttag tcagtggcat tactagcttt ggctttttcg tcgaattaga
     9181 aaatacggta gaaggattag tccatgtgga agacctaaaa gatgactact accatatcag
     9241 ggaggacatc catgccctgg taggagagag aaccaataaa agatatagat taggtgacga
     9301 agtcaaagta aaagtgatcc gggtcgaccc ggaagaaaga gagattgact ttatcctggt
     9361 tgattgaggt aaggagtggt aagatgagtg atgatataaa agtgatagca agaaataaaa
     9421 aagcaaaaca tgactaccat attgaggaga cttatgaggc cggaattata ttaaaaggta
     9481 ccgagattaa gtcgataagg aatagaaatg taaacttaaa agacagtttt gctatggtag
     9541 aaaacggaga agttttctta tataatatgc atataagccc ctataaacag ggcaaccgct
     9601 ataaccatga tccagaaaga aagagaaaac ttctattaca caaaaatgag attagaaaac
     9661 taatcggtta tactaaaaga aaaggctata ctttaattgc tttaagtgtt tatttaaaga
     9721 acaacctggc caaagttgag cttgccttgg ccaagggtaa aaatgtgcgt gataaacgga
     9781 gagatattgc taaaaagaca gctgaaagag atattgaacg tgcttatcgc aggcgactaa
     9841 aaggtaaaga ctattaatct taagcggggc ttgccccgtt taatttttac tttaaataat
     9901 gaggagggtt gtaaatcttg tctgaaacag aatattattc agagaattta agaccgcaaa
     9961 tacacttttc acccccgaaa aactggatga atgatcccaa tggccttgtg tattacaagg
    10021 gtgagtatca cctcttttac cagcataatc caactgaaac cagatggggg aatatccact
    10081 ggggacacgc tgtaagtaaa gaccttctgc actgggaaca tcttgatata gccctgaaac
    10141 ccgatgaata tggtttgatg ttttcgggta gtgctgtagt tgataaagat gacactagtg
    10201 gtttttttga tggtgaggac ggactggtag cgatctatac gactgccctg cctcagaaaa
    10261 acaaagattt ttacctccaa cagcagtgta ttgcctatag taaagataaa ggccggacct
    10321 ggcataagta tgaaggtaat cccgttctcg agaacgagaa ctggcagaac ttccgtgacc
    10381 caaaagtatt ctggcatgat gaaacagaca gctgggttat ggttgtaaca caaggtaaaa
    10441 aggttaattt ctatcgttct gatgatttaa agaactggga ttttgtcagc gatttttcct
    10501 gtaacgcctt ctattttgaa ggggtctggg aatgtccgga tttaataaaa ttaccggtgg
    10561 aaaatgaaga tgaagccaaa tggctgcttc aggtcggtgt tcaggataac agccgggccg
    10621 gtggttccgg tgtccagtac tttgtaggag agtttgacgg ctatgaattt acaaatttaa
    10681 atcctgacca gaagaccttc tgggttgact atggacagga tttttatgcg gttcaatcct
    10741 ggtttaactt accaactgaa caaaacagag agatctggat tgcctggatg aacaatttag
    10801 catattcggg agccattccc accaatgact ggcggggtgt cctttcagta ccgcgtgaga
    10861 tcgatcttaa aaaattcaat aacgaatact atctaaaaca aaaaccgatt aaagaacttg
    10921 ataacctcaa agagagggaa gacagtattt tagaacacga cctggttaat gacttcacct
    10981 gtaaactgga ctttgaccct tttgaggtta acttccaggc caccatagat tccaaggaca
    11041 agttaaatgt taacttgatt agaggtaata atttaaagat aaatttactt tatgatagaa
    11101 agaataggga agtagtattg cgaagatcct ttagaattga taaaaagatg ggtcagtttt
    11161 ttaatgatac ccatacctct ttaattgaag aggataagga aaaggtagat atccagttaa
    11221 tcatggaccg ttccacaata gagatcttta ttaatggcgg cgaaattgtc tttagtgacc
    11281 tgatcttccc ggaagaggat ggtagtacaa tcttttctat cataaccgac agcaaacagg
    11341 gtaaagtcta tgatttaaaa attaataagt tagagagtat ctggagttaa aaatataaag
    11401 ggggtcaaaa tgaaaatata tacaagaagt ggagataagg ggaatacaag tctttttgat
    11461 aattcaagag taccaaagga ttcaatccgg gtagaaagct atggaacttt agatgaatta
    11521 aattcccagt tggggatggc ccgcagtatg ttaaaaaaca aggaaatagc cggaaccata
    11581 aaaaaaatac agcgtcaact attcaatgta gccggtgaac tggctacacc caatagtgaa
    11641 gattttccag aaagggttaa gccggaagat attgaagaat tagagggaat tattgataat
    11701 cttttagata agatgaataa agaacagctt tcaaagttta tcatccccgg tagtaataga
    11761 tgttcggccg ccctgcatgt agcccgtacc atctgtcgga gggcagagag aaggattatt
    11821 actttagccc gagaggaaga gattagagaa gagctgctta agtatgtaaa caggctttct
    11881 gatgtcattt actcattggc ccgttatgtg gaaagtgaac ttgattacgt agatttcaaa
    11941 aaagagtaat ttaaaagggt ggataagatg cgttttgatg aaagagatat tatgtttgct
    12001 cggataaact atgaaccggg cactaagata tatgaagagt actatgagaa aaatccagag
    12061 aaaaaagagt ttgatgactt tttaaggtct ctacccggtt ttatggtcga agaggcattt
    12121 tcatttgacc ctctcaactc cgggctggta aatgggacct ataacttttt aagtgattta
    12181 aaaaagcagg ctgaaggaga agcaagtgaa aacacttatc aggtagagca agatgaaata
    12241 agtaaaaagt taaaacagat tgccaaatac tacggggctg acttggttgg tataactgaa
    12301 atgaaagacg agttctatta taaccaccgg ggtcgggaaa aagctcacca cggtaaaaaa
    12361 gttaccaaca aacacaaata cggtattgtg tttgccgttg aactagagaa agatatggtc
    12421 aatagggctc cccgtctggg acagtcctta gagacttcga aaggatatgt aaaggccggt
    12481 atcattggca tgatcttaag ttattatttg agagaattag gctatgaggc tagaaatcac
    12541 atggacggca actatctcct catggcacct ttcacggcag aggcagccgg actgggtgaa
    12601 atcggccgga tgggtatttt agtaacaaaa gaatacggcc cccggattag actgggagtt
    12661 gttacgactg accttaaatt aaatacagac caaagggatc ccttcggcat gaaagaattc
    12721 tgtaagattt gtaataagtg tgcggataac tgtccttctg atgcaattga ttcaggctcc
    12781 cccggccagt tgaatgggga agccagatgg aagaccgaag ttgaaaaatg ctttaaagta
    12841 tggcagatgt caggcacgga ctgcggcatc tgtattgcga gctgtccttt ttctcaaaat
    12901 ctagattttg ataagatcaa taagatgaag gataatccca aagttatgag agatattttg
    12961 aggaagtata atcaagaaca cgaggaacgc ccttataatg aaagcgaaca tgaatggcta
    13021 taagaactta acaagctaat tgagagtgag tggatattcg tgaaaaaacc aggctatatt
    13081 tttttaatat ttttaattat cttacccgta tttattttca atgtacaggc agctgaaatt
    13141 aattttatta aaaaggacaa actgataact gaacttaatc tagatagaaa tattgaaatc
    13201 attgataaaa gatttattga tttaacaggt gatgggataa aggaacaggt atttttaatt
    13261 ggtcaaaggc cggccgggcc cgattcctta ttttatgcac ctctgaaagt gattgtttat
    13321 gatatttata aaaaagaatt ctatgtgaac tcatatgaca actttaacgg ctatgaaccc
    13381 caaatggcct tttatgattt tacgggtgac caaataaaag atatctttat tgaagcaaat
    13441 acaggcggca gcggcggtat ctataaacat ttaatcgcta ctgttttgga taatgattta
    13501 aagataatat ttaatgaaag ccataataag ccgctccata tggcaggtaa ttatgaagat
    13561 aactttaaaa ctatccttag aatcaatgaa atagataaga acttaagatt agatttatct
    13621 tttaatagaa ataaatatgt ccaggaaggt atttataatt caaatggtac tttgcaaaga
    13681 gaaatacaac cttataccta tccttttggt cagttaaaac cggttgacta tgatttagat
    13741 caaaccatgg aactcaaggg aatccagagg attgttggag cctatggagc tgatacaatc
    13801 ggccatctgg aaactatttt aaattatgac ggcagctggg agatacttga agttcaaatc
    13861 agtacctatc taaaaaaata tgacccgagc cgcagtggat ccaacggtga ggttagttat
    13921 aaaataaaac aaaatgcaat aacaagtggg attaaagaaa tctactatcc ccagattact
    13981 gacttaccca aagatgtaaa ttcagtctat atcaacaaac agctacatga tatagtaaaa
    14041 cctttcttgg ctgaaagtaa tgaaatgagt atcgattatg atttcactgt aaagaataaa
    14101 aaattattca gtgttaaatt cagcggttat caaaaacatg agggcggcca gtatgagatt
    14161 ctaaaatcat ttaactatga tataaaaaat aatagaaggt tgactctatc caatatttta
    14221 aaagatgaaa cacatgataa agataaggta aaccaaatta ttaaagataa tattgaagac
    14281 aaagatttaa gagatacttt tcccggcctg tcagactgga tgggctttta tctgacaaac
    14341 agtgatttgg tcttatacta tctgagagat gattttgaaa ccaaatttaa taagatcaga
    14401 gtccccttaa gaaaattaag gcaatattta agttttgaag ttgatattca aagggatgaa
    14461 acagaaatat ttataataga ggttgattaa gatgggaaac aataaaattg ataagattaa
    14521 agaatacatt gataaaaacc aaaagctaaa tttggaggaa gtattatcag agtttgatga
    14581 aaattccact cctatcattg ataaaagaga agataattat ctattatctt ttctgtatga
    14641 agaaaatgat cttaaaaaca tttcagttgt tggttctttc cccggttttg atttgagaaa
    14701 ccaaaaatta tttaaaatag gggattcaaa gatctggact cagagttttg aagtagatca
    14761 gccggttaat tttacctatg gctttattaa aaattatgat gaagatgacc gtgagctgga
    14821 tatcaggtct gaaatgatta aagatccttt caatgaggaa gagataacaa tttatcaagg
    14881 ggaagaccag attttgaatt tgtccagtta tgacatggtt tatgataaag aagttgacag
    14941 gtactttaat aaaaagtatg aaggggaacc tcttatagaa gaaaatgaca ttaaaagtga
    15001 aatatatgaa gatgaacgaa ggtactggac cttagaaagt acccaggata aagattatga
    15061 aggtatcttt ttatgtacag acggctttac ttatattaat gacaatcagc tcttaaaagt
    15121 actcgataat ttaaggaaag aaaatttaat tcccaacttc ttatttgttt ttgttgaaaa
    15181 taaagatagg ggagttgaac tggcggccga tcaggatttt aaaaactttt taaatgatga
    15241 gctgttagga gaaataaaag aagaatacaa tatcaagcta aaagaacacg ataacattat
    15301 ctgtggcaag agcctggggg gtttaaccgc cttttatact gcctatgatt cagagctatt
    15361 taatagtatt atcagtcatt ccggtacctt tatctgggg
//
LOCUS       DATGCJ010000184        15534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_428490, whole genome shotgun sequence.
ACCESSION   DATGCJ010000184 DATGCJ010000000
VERSION     DATGCJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428490"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VKN64_08615"
     CDS             <1..160
                     /locus_tag="VKN64_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMA60339.1"
                     /translation="YGFIEREDGDDVFVHFSAIQDDGFKTLNEDQEVEFEIVEGDRGL
                     QAENVVKL"
     gene            complement(312..1490)
                     /locus_tag="VKN64_08620"
     CDS             complement(312..1490)
                     /locus_tag="VKN64_08620"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HMA60340.1"
                     /translation="MKEVVIASAARTAIGSFGKGLKDVPVVDLGTKVIESVLDRAKVD
                     PKVVDDVKMGIILQAGQGQNPARQASLKAGLDVDVPAMSVNILCGSGLKSVNLAVQEI
                     LLGDADVVVAGGMESMSRAPYLLDKARYGYKMGDGKVVDSMIKDGLTCAINEYHMGVT
                     AENLADKYDISRTRQDEFAAESQQKAIEAIDNDRFQDEIVPVEIPQRRGEPEVFKKDE
                     HPRRGVTADGLSNLRPAFKKDGTVTAGNASGINDGAAALLVMSKEKAEELGVKPLARI
                     VSYSTAAVDPKIMGIGPVPSVKKALAKCDWDFEDIQLVELNEAFASQSLAVLEELPFD
                     PNIVNVNGGAIALGHPIGASGARILVTLLYEMEKRDLTKGLASLCVGGGMGVTTIVER
                     "
     gene            complement(1718..2215)
                     /locus_tag="VKN64_08625"
     CDS             complement(1718..2215)
                     /locus_tag="VKN64_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60341.1"
                     /translation="MKLKSLAILLMVSVLILVPAAVSAQDGTSAASQPQNDEQLMEAM
                     SADGTWIIIFTEDYKTDKDLVMAGEHKNRGEVDRKLALYASDSDHNVTERYTLEAPSI
                     TVKSPKTRFQVGTFVGDVYVEANNFSLRQGFTVDGNIYFANEEALATFTIQGGAEVTG
                     DLTLE"
     gene            2432..5653
                     /locus_tag="VKN64_08630"
     CDS             2432..5653
                     /locus_tag="VKN64_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HMA60342.1"
                     /translation="MFGNVVVRTKFLPPYYKSKIFSRDHLRDLFDSTSKYPVTMVKAG
                     PGYGKSAYLTFGFRNKENVFWYNVSKGDGDVLTFLINLIYTFNTQDSEIGEKTLNFIK
                     EKGETNQEWTPVLNSLINELWDQYRYDEVYLIIDDYHLLNNQPKVNKLMEHFIDHRPP
                     NMHFIFSTRKTPDLSSLSMWRVKDQILEIDEDDLKLSKDEIRKYYTEHYDYEISEEQL
                     ETIYNLTEGWIIGVEMIRQGYKEDSKLNLVREESMDSLEHLFNYLAKEILSKQNVEVR
                     NFLIKTSILRILTPEVCDFIREKDNSAQILKYLVKNDLFIIQSDDDKYRYHHLFHDFL
                     KERAKEELNNISLLQKDIAEYYQKKDQFEEAVYHNIKSGDYFTAAKILKEKANKLMNA
                     GRFTVFDNWFEKLPDRILENFPRLYLYKGDIDRFTCNYNDALDWYRQGRKYLKENDDD
                     KGISMAYQKMAMIYLDTVQPSEADKYLKKALELREKESYWDESVLLRLVAENYLNRGN
                     VDKAKEMQDKLSKLDEENASNILDSRVKIRTGRLKEAENLLKKELKKEDIEDDKKVGR
                     AHRETVLLLSLIASFEGKNEKALEYAKTGLKKSKHLESLFTKAVANMRLGHALQINGE
                     EKQEAAIKAYEKSLEIVDKLEVRRGRAEALWGLTLVYGNLGYEEQMKQYGKEGLKICE
                     EAGDEWLGSLIQLSLGIGYIFLDNYKLATEWLTKAVKSYDYCNDIFGKTIGQIWLTLI
                     DCKLERKVDFKHRLEEIVPIIKDNDLEFVFKKVNLLGPRDQATLLPILTKGYNMDIEK
                     EFCKLILEDLEIEPGVKHPGYTLKVKTLGQFKVKRGRTEVNDSDWKRKKARTLFQIFL
                     TYQNELIPKERLAELISPEKDQKSAIRDFKVALNALNNVLEPNREARATPYFINKNGT
                     RYGLNPNATYKVDKNQFEELIQKGTEQADRGEENEAIENLSQAVKLYKGVYLPNCLYQ
                     DWAREVREKYKQLFIQGVEILAELYFELGEYQKTVEMAEKLIAEESSIEEAYQLMMKA
                     YDEMGQRSQALRTYQRCADNLEQELGVIPTQKTIELYKDIKTS"
     gene            5878..6888
                     /locus_tag="VKN64_08635"
     CDS             5878..6888
                     /locus_tag="VKN64_08635"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HMA60343.1"
                     /translation="MKENVGILSTGVYIPDKYMDSEEIAEKTGIPEDVIRKKFGITKK
                     PIPGEDDHTCEMGIKASNTAIEKAGIDPEEIDLVIWAGAEHKEYPMWTASIKLQHEIG
                     AYNAWAFDIALRCGTMIMAMKVAKDMLLNNDEMDTVLIGSGYRNGDLIDFDNPKVSFM
                     YNLGAGGTSVILRKGLDENTILKATARTDGSLSETVVVPAGGTKKDLTAEGLENNEHH
                     LDVLEPEFMKERLNEVSIENFVGVVKDSVKRSGYEMDDVDYIAILHMKYSAHKFMLDQ
                     LGLTEEQSIYLDEYGHIGQNDQIISLELALDEGKVKPGDVVVFVSAGIGYAWNALTIK
                     WG"
     gene            6923..7663
                     /gene="fabG"
                     /locus_tag="VKN64_08640"
     CDS             6923..7663
                     /gene="fabG"
                     /locus_tag="VKN64_08640"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF004198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMA60344.1"
                     /translation="MRLKDKVAIITGAGSGIGRAGALKFAKEGAKVVIADFDPEAGKE
                     VAQEINENGGEALFINVDVSDWDQVQDMVDKTVEKYETIDILINNAGVTADSFLTKMS
                     HDQWNKVVDINLKGVFNCTKAVAPLMMEKGEGKIINTSSVVGEQGNIGQTNYSATKAG
                     VIGMTKTWAKELGGKGVNVNAVAPGYIMTEMVAKIPEKVLDKLRAKVPFGELGDPEDV
                     ANAYLYLAAESGDYCNGTILDVNGGITL"
     gene            7691..8983
                     /locus_tag="VKN64_08645"
     CDS             7691..8983
                     /locus_tag="VKN64_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HMA60345.1"
                     /translation="MDFQELYNEKLITIPEAVSKVESNMKVATAMAGSEPQGLLTELG
                     KHKDELENVNVVSCLMMGDYEFLKPEMKGHFLNETWFYGPGDRANHKHGNVTYIPNNL
                     HEAGTMKIENDDINIFWGTATSMDKNGFFSLSLGLTYEKQMVENIDMVVLEINENLPW
                     TLGDTHVHISDVDFVVENNAPLIELPYIEPGEVEEQIGQNVADLIEDGSTLQLGIGGI
                     PNAITHHLTDKKDLGIHTEMFTDGMVDLFKQGVITNKKKTIWKGKMVGTFALGTQKLY
                     DFIDKNLAVEFQQGKVTNDPYILGQNHKMVSVNTALQVDVTGQVCSEAIGNKHYSGTG
                     GQVDTHRGAQRSEGGKGIIALRSTAKGGEISTIVAQLPPGSKITLGRNDIDYIVTEYG
                     VAHLKGKSIRDRVDAMISIAHPDFRDEIREKANELKIW"
     gene            9094..10335
                     /locus_tag="VKN64_08650"
     CDS             9094..10335
                     /locus_tag="VKN64_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60346.1"
                     /translation="MKRNSLLIISLVFVLAFAFSLGVVAQDDPIKIGAVNPLGDITGR
                     QSTNAMELAVKEINDNGGLLGREVELIVVDSEFKPEKGASAIDKLATVDNVDFFVGGM
                     SSGVHQAQIPSLKKYKKITVWAGAASHKAEEAIGADADWYFHLHPWDYQQGASYGRGW
                     TAIADKYPEVEVGKVFLAYEEGAFGTDSYNAYQSMFESAQEGEGPFSDIMEEFAGASF
                     KSAALGGGEYRAMLRQAKEYDPDLFIWAGYGADAVPLLAQSREVGFEPPLYVGAPPGW
                     PADFEANPLSDKVVLYGMWAPALRQVSEPAAHFWEEYIKMHGERPATYFAPLGYTNIM
                     YLAKAIREAGTIEKEALITALENTTYDSPMGTTLEMSSSNIIKHQGFTAQKILQYQDG
                     KQHVIWPFDVATAELEYPFSW"
     gene            10417..11340
                     /locus_tag="VKN64_08655"
     CDS             10417..11340
                     /locus_tag="VKN64_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA60347.1"
                     /translation="MSFSLKKTYTILLFLAIFMIIVGIWNPHALMDGLQRGSLYALIA
                     LPLALVLGILGVLNLAHGEFLTVGFYFIYMLNSRFGMDPLAAIVPTVIFLVILGGIIY
                     LLTVRQVVNEGHLSQLLITFGVSLVLLEIINIIWTSRARNVYTNYAYASTEIAGFRFG
                     TYELLYVVAAIAVLIGLQLFLKKTRLGQATFAVGQNPKGAKIVGINVEYVYLFVFSVA
                     VGILGIVAGTMLPRTSIFPAVGSPFTLKSFALAAMAGLGNLNGILFAGILLGVGESAI
                     NYIPNYGGWSDIVFFGVLIIVIIARSYRGAR"
     gene            11340..12284
                     /locus_tag="VKN64_08660"
     CDS             11340..12284
                     /locus_tag="VKN64_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HMA60348.1"
                     /translation="MKKNLFILILLIIAATLPLYAGAYLMSVFTTILIFMSLSLSWDM
                     MLRTGQLSFGTAGFFGLGGYASALIVAQLGLNPILSLLVGAIFAGLVAFGLGFIVLRL
                     RQIYFAITTLALTHVFSVIIRNMEDLTGGASGTQLSTAIFEGNPTKIYYMILIIALLV
                     IFISEVFKRSRIHYAINAIRNDETTAKSSGIDVFKYLLFVFVVTSAIQGLVGAAYAQQ
                     YAFVNPESTFSLNFLLLPLAMALVGGIYSTNGPIIGALVLGIISEYLKLIMPYGHLIV
                     YGIIIVIVVLFLPQGVYKPIRNKFLKRSIVGGDNNAHT"
     gene            12271..12987
                     /locus_tag="VKN64_08665"
     CDS             12271..12987
                     /locus_tag="VKN64_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60349.1"
                     /translation="MPTLKAENIVRRFGGLVAVDNLSFEVDKGEILGIIGPNGAGKTT
                     VINLIAGTLAVNKGSIKFNGEDVTNNPAHIRNRKGIARTFQIVRPLENFSAIENIMVG
                     SLFGEGEKLSDARNSALEICDFLKLENVNKSIDELTVLELKKVELGRALASDPELLFL
                     DEIMAGLTSEETWELIDIVKKLHERDIAIVVVEHIMKVVKELTHRVIVLNKGATIAEG
                     PYEKVSQNKEVIAAYLGEEE"
     gene            12989..13696
                     /locus_tag="VKN64_08670"
     CDS             12989..13696
                     /locus_tag="VKN64_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60350.1"
                     /translation="MLEINNLNVSYGRIQVIWDLTMRIEDNEAVSIFGSNGAGKTTLI
                     KAIMGLVKNKAGSIKFKGEEISDLATHEIVKKGIALVPQKRELFPLMTVKENLKLGAA
                     YIPAAKDKTEESLEFVYNIFPILREREKQLAGTMSGGQQRMLALGRALMSNPKLLILD
                     EPSVGLQPSLVTELFKKMDEIKKEDVSILLAEQNVKQGLKIIDRGYVLENGRIELEDT
                     VEGLSSNEEVQKTYLGI"
     gene            13729..14721
                     /locus_tag="VKN64_08675"
     CDS             13729..14721
                     /locus_tag="VKN64_08675"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HMA60351.1"
                     /translation="MGVKLIATGSYAPDNTYDIEEMSEIVGEDISEFMEPLGIEQKNV
                     TGPDMSAADLAVKAGQEALEKAGMSAEEIDLLILSTDTPDIISPQSSAIVANDLGMRD
                     GAPFFDLNASCTGFIFGTETAKNWIEGNPDFNTVMLISTYNMTKYADMERGKFFCVFS
                     DGAGAAIYQKDETKRGVLESHFIGNGDQWMTLGIYVGGTRFPATEERIKGEGDVPPDL
                     TFWRGELMNRNPELWPVIIEKMLDKSNYELEEVDKFIFTQINKSAIALTCDNLGIDME
                     KTHNVMDKYGYTGNACIIMALNDALEKGEVEEGDLVCLTASGVGYTMATVLIRI"
     gene            14863..>15534
                     /locus_tag="VKN64_08680"
     CDS             14863..>15534
                     /locus_tag="VKN64_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HMA60352.1"
                     /translation="MQILDQVVFMMSPDNEPIASCKSKEEVIFKTQDCFGGQIETEDM
                     TLDELDWDNINPATGPLYVKGAEVGDVLKVTIKEINIADKGSMTTIPEMGYLSEFLTG
                     PETKVVHIEDNKAVFNDKIKLPLKPMIGVIGVAPPVKSIDCGTPGSHGGNMDNKVIRA
                     GSTVYFPVNVPGALLAMGDMHAVMGDGEVVICGVEIAGEARVEVEVIKEKNINNPMVE
                     DQEAFY"
BASE COUNT         5202 a         2385 c         3253 g         4694 t
ORIGIN      
        1 ttatggattt attgaaagag aagatggaga cgacgtattt gttcattttt cagctattca
       61 agatgatggt ttcaaaactt taaacgaaga tcaagaagta gaatttgaaa tcgttgaagg
      121 cgaccgcggc ctacaggctg aaaatgttgt taaattataa attgttataa gttatagcaa
      181 ctaattcccg acttgttaat aagtcgggtt tttttaatga attaaatatt tcaaagaatg
      241 aaaaaacctg tttggaagcc ccaaacaggt tttttattaa ttatatataa tctatatttt
      301 ttattaaata attatctttc tacaattgta gttacaccca taccgccgcc tacacataag
      361 gaggcgagtc cttttgttaa gtctcttttt tccatctcat ataataaagt aaccaagatt
      421 ctagcaccac tggctccaat tggatgaccg agggcaattg ctcctccgtt aacatttact
      481 atgttgggat caaagggcag ttcttctaat actgctaagg attgagaagc aaaggcttca
      541 tttagttcca ctagttgtat atcttcaaag tcccagtcgc attttgccag ggctttctta
      601 actgaaggta caggaccaat tcccataatt tttggatcta cggcagctgt agagtaagaa
      661 acgattcggg ctaaaggttt tactcccaat tcttcagctt tttctttact catgaccagt
      721 aaggctgctg ctccgtcatt aataccagat gcattaccgg cagttacagt accatccttt
      781 ttaaaagccg gtcttaaatt agataatccg tcagcggtaa ctcctctacg gggatgttca
      841 tcttttttaa atacttctgg ttctcccctg cgctgaggaa tttctaccgg tacaatttca
      901 tcctggaaac ggtcattatc aatagcttca attgctttct gctggctttc agcagcaaat
      961 tcatcctgtc ttgtccggct gatatcatat ttatcagcta aattttcggc agttactccc
     1021 atatggtatt catttatggc acaagtaagt ccatctttta tcatggaatc tacaactttg
     1081 ccatcaccca tcttgtaacc gtaacgggcc ttatccagta aatagggggc tctgctcata
     1141 ctttccattc cacctgctac taccacatct gcatcaccta ataatatttc ctgaacagcc
     1201 agattaacag atttaagtcc tgaaccacat agtatgttga cagacatggc tggtacatct
     1261 acgtctaagc ctgcttttag ggatgcctga cgggcaggat tctgaccttg accggcctgt
     1321 agaataatac ccattttaac atcatcaacg actttaggat ctactttagc tctatctaaa
     1381 acagattcta ttactttagt gcctaaatct acaacaggaa catcttttaa ccccttacca
     1441 aaagaaccaa tagcagtacg tgctgcactt gctataacaa cttctttcaa aagaatccct
     1501 cctattaata attatgtaat caattctatt atatattgta ataaaaaggt gttacccata
     1561 agttacaata tagttgcatt aataattagt attatgtaaa attaattaat tacatgatat
     1621 tatgatacaa aaaataaggg gagaagtaaa taaaaaaacc gacttatcat tataaaaatg
     1681 ataagccggc tttgaattat agatataatt atttaattta ttctaaagtt aaatcgccag
     1741 ttacttctgc tccaccctga attgtaaagg tagctagagc ttcttcattt gcaaagtaaa
     1801 tattaccgtc aacagtaaat ccttgtctta aactaaagtt attagcttct acgtatacat
     1861 caccaacaaa ggtaccaacc tggaatcttg tcttagggct tttaactgta atacttggag
     1921 cttcaagggt atacctttct gtaacgttat ggtcgctatc tgaagcatat agagctaatt
     1981 tgcggtcaac ttcgccgcgg tttttatgct cgcctgccat tactaaatct ttgtcagtct
     2041 tatagtcttc tgtaaagatt ataatccagg taccgtcagc actcattgct tccataagtt
     2101 gttcatcatt ttgtggttga gaagcagctg aggttccatc ctgggcacta actgccgctg
     2161 gtactaaaat taaaactgaa accattaata aaatagctaa actttttaat ttcatgatta
     2221 ttcctcctat tatgataatt ttactcaact ttctattata ataacttatt gtgaaaaaat
     2281 tgtcaagtta tatacagata ttaataacta aaacatgaaa ttagttaatt ttaacaaatt
     2341 taataaggaa attcttgtta taattagagg aattttgtct aatttattga atttattttt
     2401 tacaagaata aaattaagga gaggtgtaac agtgtttgga aatgtagtgg ttagaacaaa
     2461 gtttcttcca ccttattata aaagcaaaat attttcaaga gatcatttaa gggatttatt
     2521 tgatagtact tcgaagtatc cggtgaccat ggtgaaggcg ggtcccggct atggtaaaag
     2581 tgcttatcta acctttggct ttagaaacaa agaaaatgtc ttttggtaca atgttagcaa
     2641 aggtgatggc gatgtattaa catttttaat aaatctcata tataccttta atacacagga
     2701 ttcggaaata ggggagaaga ctttaaattt tatcaaagaa aaaggtgaaa ccaatcagga
     2761 atggacccct gtcttaaatt cacttatcaa tgaactctgg gatcaatatc gttatgatga
     2821 agtttatcta ataattgatg attaccatct attaaataat caacctaagg ttaataaatt
     2881 aatggaacat tttattgatc ataggccacc taatatgcac tttatttttt ccacccgtaa
     2941 aactcccgac ttatcttcct tgagcatgtg gagagttaaa gatcaaattc tggaaattga
     3001 tgaagatgat ctaaagttaa gtaaagatga gataagaaaa tattatacgg aacattatga
     3061 ctatgagata agtgaagaac agcttgaaac tatatataat ttaacagaag gttggattat
     3121 cggggtggaa atgattagac agggttataa agaagacagc aaattaaatt tagtgagaga
     3181 agagtcgatg gattctctag aacatttatt taattatcta gctaaggaaa tactatctaa
     3241 acaaaatgtt gaggtaagaa actttttaat caagacttct atcttgagga tattgactcc
     3301 ggaagtttgt gattttataa gagagaaaga taatagtgct cagattctaa agtacctggt
     3361 taaaaacgat cttttcatta ttcaatcaga tgatgataag tatcgttacc accatctatt
     3421 ccatgacttt ttaaaggaaa gagccaaaga agaattaaat aatatttctc ttttgcaaaa
     3481 agatattgcc gagtattatc agaaaaaaga tcaatttgaa gaggcggtct atcataatat
     3541 aaaatcgggt gattacttca cggctgctaa gatcttaaaa gagaaggcta acaaattaat
     3601 gaatgcaggc cgttttacgg ttttcgataa ctggtttgaa aaactgccgg acagaattct
     3661 ggaaaacttc cccaggcttt atttatataa aggagatatt gaccgtttta cctgtaatta
     3721 taatgacgcc ctggattggt ataggcaggg acggaaatat ttaaaagaaa atgatgatga
     3781 taaagggatc agtatggcct atcaaaagat ggccatgatt tatctcgata ctgttcaacc
     3841 ttctgaggcc gataaatatc taaaaaaagc cttagaatta agggaaaaag aaagctattg
     3901 ggatgaatca gtcttattaa ggctagttgc tgaaaattac ctaaataggg gtaatgtaga
     3961 taaagcaaaa gagatgcagg ataaattaag caaattagat gaagaaaatg ccagtaatat
     4021 cttagattca cgggttaaaa tcagaacggg acggcttaaa gaagctgaaa acttattaaa
     4081 aaaagaactt aaaaaagaag atatagaaga tgataaaaaa gttggtaggg cccaccggga
     4141 aaccgtctta ttactttctt taattgcttc ttttgaaggg aaaaatgaaa aggctttgga
     4201 atatgctaaa acgggtctta aaaaatcaaa acatctcgaa tctttattta ctaaagcggt
     4261 tgccaacatg agattaggac atgctttaca gattaatggt gaggagaaac aggaagctgc
     4321 gataaaagca tatgaaaaaa gcctagagat cgtagataaa ttggaagtaa ggcggggccg
     4381 ggccgaagca ctctggggtc tgaccttagt ctatggcaat ttaggttatg aagaacagat
     4441 gaaacagtac ggaaaagaag gtttaaagat ttgtgaagaa gccggggacg aatggttggg
     4501 tagcttaatt caactctccc tcggaatcgg atatatcttt ttagataatt ataaattagc
     4561 aaccgaatgg ttaactaagg cagttaagtc ttatgattac tgtaatgata ttttcggcaa
     4621 gacaatcggc caaatctggc ttactttaat agactgtaaa ttagaaagaa aggtagactt
     4681 caaacaccgc ttagaagaga ttgtaccaat aattaaagac aatgatttag agtttgtatt
     4741 taaaaaagta aacctcttag gaccgaggga tcaggcaact ttattaccca ttttgacaaa
     4801 gggatataat atggatattg aaaaagagtt ctgtaaatta attctggaag acttagaaat
     4861 agaacccggg gttaaacacc ctggttatac cctgaaagta aaaactctgg gacaatttaa
     4921 agttaaaagg ggtagaacag aagttaatga tagtgactgg aagcgaaaaa aagcaagaac
     4981 tttatttcaa atctttttaa cctatcaaaa tgagttaata ccaaaggaaa gattagcaga
     5041 acttatttcg ccggaaaaag accaaaaatc agcaatcagg gacttcaaag ttgctttaaa
     5101 tgccttaaat aatgtattgg aaccgaatag agaagctaga gccaccccat atttcataaa
     5161 taagaatgga acccgctatg gtctgaatcc caatgctacc tataaagtgg ataaaaatca
     5221 atttgaagaa ctcatccaaa aaggtacaga acaggctgat cggggagaag aaaacgaagc
     5281 tattgaaaat ctttctcagg cggtcaagct gtataaagga gtttaccttc ctaattgttt
     5341 ataccaggat tgggcccgag aagtgcgaga aaagtataaa cagctattta ttcagggagt
     5401 tgaaatttta gctgaacttt attttgaatt aggagaatat caaaaaaccg ttgagatggc
     5461 agaaaagcta attgctgaag aatcttctat agaagaggcc tatcaactga tgatgaaagc
     5521 ttatgatgag atgggacaga gatcccaggc gcttagaacc tatcagcgtt gtgctgataa
     5581 tctagaacag gaattaggag ttattccgac ccaaaagact atcgaattat ataaagatat
     5641 aaaaacaagt taaatatagt atataaaccg gcaagaatcc aggccattgg cctggatttt
     5701 ttatttgtaa atactttgta actttttgca actgtattgt aacttatctt tattataatt
     5761 gagatagttg atttaaggat aactgaagat agatataact ttgtaactgc aatgcaacat
     5821 tgtagtaact aattttaatt ataatattaa caacaaatat taaatggggg tgaaatggtg
     5881 aaagaaaacg ttggtatcct ctcgactggt gtatatattc cagataaata tatggacagt
     5941 gaggaaatag ccgaaaaaac aggtattcca gaagatgtaa taagaaaaaa gtttggtata
     6001 actaaaaaac cgattccagg tgaagatgat catacctgtg aaatgggtat aaaagcttct
     6061 aatacggcaa ttgaaaaagc tggtattgat ccggaagaga tcgatttagt aatttgggca
     6121 ggggcagaac ataaggagta tccgatgtgg actgcttcca ttaaacttca gcatgaaata
     6181 ggtgcataca atgcctgggc ttttgatatt gccttacgct gtggaacgat gattatggcg
     6241 atgaaagttg ccaaagatat gctgcttaat aatgatgaaa tggatactgt cttgattggt
     6301 tccggttatc gaaacggaga tttaattgat tttgataacc ctaaagtatc ttttatgtat
     6361 aatttaggag ccggtgggac ctctgtgata ttaagaaaag gtttagatga aaacacaatc
     6421 ttaaaagcaa ctgcgagaac agatggttct ttatcggaaa cggtagtagt accggctggt
     6481 ggaactaaaa aagatctaac ggcagaggga ttagaaaaca acgaacacca tttagatgtt
     6541 ttagaaccgg aatttatgaa ggaaaggcta aacgaagttt ctattgaaaa ctttgtggga
     6601 gttgtcaaag attctgttaa aagaagcgga tatgaaatgg atgatgtaga ttatatagca
     6661 atccttcata tgaagtactc ggcccacaag tttatgcttg accagttagg tctgacagaa
     6721 gaacagagta tctatctgga tgaatatgga catattggac aaaatgatca gattatttcc
     6781 ttagaattag cccttgatga gggtaaagtc aaaccaggcg atgttgttgt ctttgtaagt
     6841 gcaggtatcg gttatgcctg gaatgccctt acaatcaaat ggggttaaac ttaaaaagtt
     6901 aatattaaat ggaggttttt acatgaggtt aaaagataaa gtagctatta ttactggtgc
     6961 cggtagcggt atcggaagag ctggcgctct gaagtttgca aaagaagggg caaaggtagt
     7021 tattgctgat tttgatccag aagctggtaa agaagtagcc caagaaatta atgaaaatgg
     7081 gggagaagcc cttttcatta atgttgatgt gtctgattgg gatcaggtac aagatatggt
     7141 ggataaaact gtagaaaaat acgaaacaat tgatatttta atcaataatg ccggtgtaac
     7201 tgcagattcc tttttaacaa agatgtctca tgatcagtgg aataaagttg tagatattaa
     7261 tttaaaaggt gtttttaact gtacaaaagc agtcgcacct ttaatgatgg aaaaaggtga
     7321 aggtaaaatt attaatacct cttctgtagt aggagaacag ggaaatattg gtcaaaccaa
     7381 ttattctgct actaaagcag gcgttattgg gatgacaaaa acctgggcta aagagttagg
     7441 cggcaaaggt gtaaatgtta atgctgtagc tcctggttat atcatgacag agatggttgc
     7501 taaaattcca gaaaaggttt tagacaaact tagagctaaa gtaccatttg gagaattagg
     7561 agatcctgaa gatgttgcta atgcttatct atacctggca gctgagtcag gtgattactg
     7621 taatggcact atcttagatg taaacggtgg tatcacctta taagttttgt tccaaaaagg
     7681 aggaagatta atggatttcc aagaactata taatgaaaaa ttaattacaa taccagaagc
     7741 ggtctcaaaa gtagaatcaa atatgaaagt agctactgca atggcgggtt cagaaccaca
     7801 aggactttta actgaacttg gaaaacacaa agatgaactg gaaaatgtta atgtggtttc
     7861 ctgtttaatg atgggagact atgaatttct gaaaccagag atgaaaggac attttctgaa
     7921 tgaaacctgg ttttatgggc ctggtgacag ggctaaccat aaacacggta atgtcaccta
     7981 tatacccaat aatctccatg aagcgggtac catgaaaata gagaatgatg atattaatat
     8041 tttctggggt actgctactt ccatggataa aaatggtttc ttttcacttt cacttgggtt
     8101 aacttatgag aaacaaatgg tggaaaatat tgatatggta gttttagaaa tcaacgaaaa
     8161 tttaccctgg actttaggag atacccacgt tcacatttca gatgtagatt ttgtagttga
     8221 aaacaatgcc ccactcattg aactaccata tatcgaaccc ggtgaagtag aagaacagat
     8281 cggtcaaaat gtggccgacc ttatcgaaga cggttcaact ctccagttag gaataggcgg
     8341 tattcccaat gctatcaccc atcacttaac agacaaaaaa gacctcggta ttcataccga
     8401 aatgtttaca gatggaatgg ttgatctttt caaacaaggt gtgattacta ataagaaaaa
     8461 aaccatctgg aagggtaaaa tggtggggac atttgcttta ggaacgcaga agttatacga
     8521 cttcatcgat aaaaatttag ctgtcgaatt tcaacagggt aaggttacca atgatcctta
     8581 tatcttaggt caaaaccata aaatggtgag tgtaaataca gccttacagg ttgatgtaac
     8641 aggccaggtt tgttcagaag caattggtaa taaacattat agtggaaccg gtggtcaggt
     8701 agatacccac cgcggtgccc agcggtctga aggcggcaaa ggtattatcg ccttaagatc
     8761 tacagcaaaa ggcggcgaga tctctactat tgtagctcaa cttccacccg gatccaagat
     8821 aactttaggt agaaatgata tagactatat agttactgaa tatggtgtgg ctcatcttaa
     8881 aggaaaaagt attagagata gagtagatgc catgattagt atagctcatc cagattttag
     8941 agatgaaatt agggaaaaag ccaatgaatt gaagatctgg taagtaaaat tagattgaaa
     9001 ggatgtgata gtggggtaat aaaatgattg catttcagta gattaatttt aagggttcaa
     9061 ttaatattat aaagatttaa gggggattat aaattgaaga gaaatagttt gttaattatt
     9121 tcgttagttt ttgttttagc atttgctttt agtcttgggg tagttgctca agatgatccc
     9181 attaaaattg gggcagtaaa cccactcggt gatattaccg gtaggcagtc cacaaatgct
     9241 atggaattgg ctgttaaaga gatcaatgac aatggtggac tattaggtag agaagtagaa
     9301 ttgattgttg tcgatagtga attcaaacct gaaaaaggtg cttctgctat tgataaatta
     9361 gctaccgttg ataatgttga tttctttgtt ggcggtatgt caagtggtgt acatcaggcc
     9421 caaattccaa gtttgaagaa atataagaag ataactgttt gggctggcgc agcttctcac
     9481 aaagctgaag aagctatagg tgcagatgca gactggtatt tccacttaca cccctgggat
     9541 taccaacagg gcgctagtta tggtcgaggc tggacagcaa ttgcagataa atatccagag
     9601 gtagaagtag gtaaagtttt cctagcatat gaagaaggtg cttttggtac cgattcatat
     9661 aatgcttatc aatcaatgtt cgaaagcgcc caagaaggcg aaggaccatt ttcagatatt
     9721 atggaagaat ttgccggtgc ttcctttaag agtgctgctc taggtggcgg tgaataccgt
     9781 gcaatgttac gtcaggctaa agaatatgat ccagatctat ttatctgggc cggttatggt
     9841 gcagatgcag ttccactact tgctcaatca agagaagttg gctttgagcc cccactttac
     9901 gtaggagctc ctccgggatg gccagctgac tttgaagcta atccactgtc tgataaagtt
     9961 gttctttatg gtatgtgggc tcctgcttta agacaggtta gtgaacccgc agctcacttc
    10021 tgggaagaat atatcaagat gcatggtgaa agaccagcga cttactttgc tcctctaggt
    10081 tataccaata ttatgtattt agctaaagca attcgtgaag ctggtaccat agaaaaagaa
    10141 gcactaatca ctgccttaga aaatacaact tatgattcac caatgggtac aaccctagaa
    10201 atgtcatcaa gtaatattat aaaacaccaa ggatttacag ctcagaagat actacagtat
    10261 caagatggta aacaacacgt aatttggcca tttgatgtag caactgctga acttgaatat
    10321 cccttttcct ggtaattagt caatcagaca gccgggctga gattggcccg gctgtttatt
    10381 attaataaaa aatttcaata aggatggagg ggaaaaatgt cattttctct taagaaaaca
    10441 tatacgatat tactattttt agcaatattt atgataattg tcggtatttg gaatccccat
    10501 gccttgatgg atggtctaca aagaggcagt ttatatgctt taatagcttt accactggct
    10561 ttagtgttag ggatcctagg tgttttaaac ctggctcatg gggagttttt gactgtaggt
    10621 ttttacttta tttatatgct taattctaga tttggtatgg atccactggc agcaatagta
    10681 ccaactgtaa tctttttagt tatattaggc ggtattatat atttattaac cgttagacag
    10741 gttgttaatg aaggtcattt aagtcaactt ttgattacat ttggagtttc attggtctta
    10801 ttggagatca ttaatattat ttggacatca agagctagaa atgtttatac taattatgct
    10861 tatgcttcta ctgaaattgc cggatttaga tttggtactt atgaattatt atatgtggta
    10921 gcagcaatag ctgttttgat aggcttacaa ttatttttga aaaaaactcg tttaggccag
    10981 gccacttttg cggtcggcca aaatcctaaa ggagctaaga tagtcggtat aaatgtagaa
    11041 tatgtatatc tatttgtatt tagtgtagct gttggaatac tgggtatagt ggcaggaaca
    11101 atgctgccta gaacctctat attcccggct gtaggttcac cttttacctt aaaatctttt
    11161 gccctagctg cgatggcagg tctgggtaac ctgaacggaa tattatttgc ggggatttta
    11221 ctcggtgttg gtgaatctgc cattaattat attcctaatt atggtggttg gtcagatata
    11281 gtattctttg gagtattaat tatagtaatc attgcaagat catatagggg ggctagataa
    11341 tgaaaaagaa tttattcatc ttaattttat taattattgc agcaacatta cccttatatg
    11401 ccggggcata tttaatgagt gtatttacta cgatacttat ttttatgtca ttgagtctta
    11461 gctgggatat gatgcttaga accggacaac tttcttttgg tactgccggc ttctttggtt
    11521 taggtggtta tgcctcagct cttattgtag ctcaattagg acttaatccg atcttaagtt
    11581 tactggttgg agcaattttt gccggcttag ttgcttttgg tttgggtttt attgttttaa
    11641 gattacgcca gatttacttt gcgattacaa ccttagcttt aacgcatgtt ttctcagtta
    11701 ttataaggaa tatggaagat ctgaccggtg gtgcaagtgg tactcagtta agcaccgcca
    11761 tcttcgaggg taatcctacc aagatatatt atatgatttt aataatagct ttattagtta
    11821 tatttatttc agaagtattt aaaagaagta gaattcatta tgcgattaat gcgattagaa
    11881 atgatgaaac aacagccaaa tcaagtggta tagatgtctt taaatacctc ttatttgtct
    11941 ttgtagtgac ttctgctatc cagggattag tcggagcggc ctatgcccaa cagtatgctt
    12001 ttgttaatcc tgagagtacc ttttcattga actttctact cttaccctta gcgatggcgt
    12061 tagtgggtgg aatatattct actaacggac ctattattgg tgccctagta ttggggataa
    12121 tctctgaata cttaaaatta ataatgcctt atggacacct aattgtttat ggtattatca
    12181 ttgtaatagt agtactattc ttacctcagg gtgtatataa accgatcagg aataagttcc
    12241 tgaagagaag tatagttggg ggggataaca atgcccacac ttaaagctga aaatattgta
    12301 agaagattcg gtggattagt tgcagtagat aacttaagtt ttgaagttga caaaggtgag
    12361 attctcggga ttattggacc caatggagct ggtaaaacaa cagtaattaa tttaattgcg
    12421 gggacacttg ctgtcaataa aggatctata aaatttaatg gagaagatgt aacgaacaat
    12481 cctgcccata ttagaaatag gaagggtatt gcgaggacct ttcaaattgt ccgtccctta
    12541 gagaactttt cagccattga aaatatcatg gtcggatctt tatttggaga aggcgaaaaa
    12601 ctgtccgacg ctcgtaattc agctctagaa atctgtgatt tcttaaaatt agagaatgtc
    12661 aataaatcaa tcgatgaact tacagtctta gagttaaaaa aggtagagtt aggcagagct
    12721 ttagcttctg atccagagct gcttttctta gatgagatca tggcaggcct aacaagtgaa
    12781 gagacctggg aactaattga tatagttaaa aaattacatg aaagagatat agcgatagtt
    12841 gtggtagaac acatcatgaa ggttgttaaa gaattaactc accgagtaat tgtactaaat
    12901 aaaggagcaa ccattgctga aggtccatat gaaaaagttt cacagaataa agaagttatc
    12961 gctgcttacc tgggggagga agaataatat gcttgagatt aataatttaa atgtttcata
    13021 cggaaggatt caggtaattt gggatctaac gatgagaata gaagacaatg aagctgtaag
    13081 tatatttggt tccaatggag ccggtaaaac taccttaatt aaagcaataa tgggtttagt
    13141 taaaaacaaa gcaggtagta taaagtttaa aggtgaagag atcagtgatc ttgccaccca
    13201 tgagatagtt aaaaaaggta ttgcactggt accgcagaaa agagaacttt tccccctgat
    13261 gacggtaaaa gagaacttaa aattaggtgc tgcctatata cctgctgcta aagataagac
    13321 agaggaaagt ttagagtttg tttataatat tttcccgatc ttaagggaaa gagaaaaaca
    13381 attagcaggt acgatgagtg gtggtcaaca gaggatgttg gcattaggta gggctttaat
    13441 gtctaatcct aaactattaa tccttgatga gccatcggta ggattacaac cttctttagt
    13501 aacagaactt ttcaaaaaga tggatgaaat taaaaaagaa gatgtttcca tcttactggc
    13561 agaacagaat gtgaaacagg gattgaaaat aattgataga ggatacgtcc tggagaacgg
    13621 taggatcgaa ttagaagata cagtggaagg attatccagt aatgaagaag tccaaaaaac
    13681 ttatttaggt atctaattag caaagaaact aagaaaggag tgttcctatt gggtgttaaa
    13741 ttaatagcca caggttctta tgctcctgat aacacttatg atattgaaga gatgagtgag
    13801 attgtaggtg aagatatcag tgaatttatg gaacctttag gaattgaaca aaaaaatgtt
    13861 acaggacctg atatgagtgc agctgatcta gctgttaaag caggtcagga ggcccttgaa
    13921 aaagcaggta tgtcggctga agaaatagat cttttgattt tatctaccga tactcctgat
    13981 atcatttcgc cccaaagttc agctattgtt gcgaacgatt tggggatgag agatggtgct
    14041 ccattttttg acctgaatgc ttcctgtacc ggttttatat tcgggacaga aacggccaag
    14101 aattggattg aaggcaaccc tgattttaat acagttatgt taatatcaac ctataatatg
    14161 acaaagtatg cagatatgga aagaggtaag ttcttctgtg tattctcaga tggagccgga
    14221 gctgccattt accaaaaaga tgaaactaaa agaggggtat tagaaagcca tttcatcggt
    14281 aacggagatc agtggatgac tctgggaatt tatgtaggtg gaaccagatt tcctgctaca
    14341 gaagaaagga taaagggtga aggagacgta ccacctgatt taactttctg gcgcggcgaa
    14401 ttaatgaacc gcaatccaga gttatggcct gtaatcatcg aaaaaatgct tgataaaagc
    14461 aactatgaat tagaagaggt agataaattt atctttaccc agattaataa atctgctatt
    14521 gcactaacct gtgataactt aggtatagat atggaaaaaa cccataatgt aatggataaa
    14581 tacggttata ccggtaatgc ctgcatcatt atggctttaa atgacgctct agaaaaaggt
    14641 gaagttgagg aaggtgacct ggtctgtcta acagcatcag gtgttggtta tacgatggct
    14701 acagttttga ttagaatata aaaataagat aggataatat agacgggggg gtggctcttt
    14761 tgggccaccc tttttagcat atgataagag gatttttctt ttctatcttg aaaagtaaat
    14821 ataattgaca ataaaaaaat tatatctttg gaggtaaata aaatgcaaat acttgatcag
    14881 gttgttttta tgatgtctcc tgataacgaa ccaattgcaa gctgtaaatc aaaagaagaa
    14941 gtgatattta agacccagga ctgtttcggt ggtcagattg agacggaaga catgacatta
    15001 gatgaactgg actgggataa tattaatcca gcgaccggcc ctttatacgt aaaaggggct
    15061 gaggtcggtg atgtacttaa agtaacaatt aaagaaatta atatagctga taaggggtcg
    15121 atgactacca taccggagat gggttattta tctgaatttt taaccggacc tgagaccaaa
    15181 gttgttcata ttgaagataa taaagcagta tttaatgata aaattaagct gcccctaaag
    15241 cccatgattg gtgttattgg agtagcacct cctgttaaga gtattgactg tggtactccc
    15301 ggttcccacg gtggtaatat ggataacaaa gtaattagag ctggttctac cgtatatttc
    15361 cccgtaaatg taccgggagc cttactggcg atgggagaca tgcatgcagt gatgggtgat
    15421 ggagaagtag taatatgtgg ggttgaaatc gcgggagaag ctagagtaga ggttgaagta
    15481 attaaagaaa aaaatattaa taatcctatg gtggaagatc aagaagcctt ttat
//
LOCUS       DATGCJ010000185         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_160510, whole genome shotgun sequence.
ACCESSION   DATGCJ010000185 DATGCJ010000000
VERSION     DATGCJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160510"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VKN64_08685"
     CDS             <1..797
                     /locus_tag="VKN64_08685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMA60353.1"
                     /translation="NINDNISINKTELKQGDILVVKTAKEININQIKFNGNQYQTKKY
                     EQGKIAIIPISYWTNAGNYELEIIKDDRNITTPINIISGNFTNSYLQVDKDQEELIKP
                     EKEATIKRKKEDQKMINQARKNSRQAIIFNNNFIWPVDGIISTDFGATRFVNGELQSR
                     HSGIDIAADKGTPVLAANGGIVKLAAHLLVTGNTIIIDHGFNIFSSYSHLSKLDVNKG
                     EQVKKGEKIGEIGSTGFSTGPHLHWTITVGRVFVNPRNFVEKDILK"
     gene            complement(1054..1485)
                     /locus_tag="VKN64_08690"
     CDS             complement(1054..1485)
                     /locus_tag="VKN64_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HMA60354.1"
                     /translation="MPRPPKRRRIKNIPYIRFFKPAGIPGRELEEVLLSLEEVEALRL
                     KDVEGLNQTESAEKMNISRPTFQRIITKAHQKVAEALLEGKALRFEGGDYELDKGRYR
                     CKECGAEFMLKINKHRKGHGRRNQGKICPECGANAVEKIDK"
     gene            complement(1555..2286)
                     /locus_tag="VKN64_08695"
     CDS             complement(1555..2286)
                     /locus_tag="VKN64_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMA60355.1"
                     /translation="MISISKPPVPFYITRFFAKGLFRTVFNLDIKGKNNIPKDRNYII
                     ISNHLNWIDPFFILMIFPSAPKIIFIAENEGIYDNAKKKKFIDVMGKPIVPINRDITK
                     SRIKAVRSMMRIIKEGNNLAVFPEGRLGHAEGQIFPFYLGVFSVAKKLDVPILPVAIS
                     GTKKLSFRNPIKIKIGELSTCKKGESDQDFAERLALKMRELLPEYKGEGPFPNTMNWL
                     TDLFQGELRPFEGEYTLIIKRDDGD"
     gene            2419..3852
                     /locus_tag="VKN64_08700"
     CDS             2419..3852
                     /locus_tag="VKN64_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60356.1"
                     /translation="MSKKDVKLKNIISYGLGDIYGGGAFLLLGTLYMIFLTDIVGLNP
                     ALAGSVVVIGKVWDAISDPLMGYISDNIDTKYGRRRIFFLIGIIPITISFISLWIPLN
                     TNSSLLLFAYYSFAYIFFSTVFTMVMVPYSALNAELSENFAVRTKLSTARMAFSQFSA
                     LISGVLPKYLIDFLNKGTIVSPHYSYLIMGIVFGVLYALPWLFVFKGTWEIPYEVDKE
                     TKFIEKIKRYKTVLYNKSFKIHLGMYISAYLAMDFLMALFIYYLTYYIQKPDIFPFCM
                     LAILLSQIVMLPVYMKISNTKGKSLAFTIGLTIWGIGMFNLLFLSASTPLYIIIIICI
                     VIGSGLSAGVMIPWAILPTISDIDQVINIKRRTGIYSGLMTFIRKIAQAVALWLVGII
                     LSLINYVPNQPQSPTTLFYLKYIFVFVPIILIGLGIFFSYKYKVTPRKQKILIEEIKR
                     IKNGGEKAGADSEVKKVCEELTGVKYR"
     gene            complement(3920..>4135)
                     /locus_tag="VKN64_08705"
     CDS             complement(3920..>4135)
                     /locus_tag="VKN64_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60357.1"
                     /translation="FLTSDLFKYNSLIIEIVNGIINEDNTTGLYNMEYGPDQGRYLKL
                     PVNNVSDETNKWIESIANEVASGEIQV"
BASE COUNT         1325 a          765 c          621 g         1424 t
ORIGIN      
        1 ttaatattaa tgataacata tccattaata aaacagaact aaaacaggga gatattttag
       61 tagtaaaaac agctaaagaa ataaatatta accaaataaa atttaacgga aatcaatatc
      121 aaactaaaaa atatgaacaa ggtaaaatag cgattattcc catctcttac tggacaaatg
      181 ccgggaatta tgaacttgaa attataaaag atgatagaaa tattacaact cccataaata
      241 ttatatcagg taactttact aatagttacc tccaggtaga taaagatcaa gaagaactta
      301 ttaaacccga aaaagaggca actattaaaa gaaaaaaaga agatcaaaaa atgataaacc
      361 aggcaagaaa aaatagtaga caggcaatta tctttaataa caattttatc tggcctgtcg
      421 atggtattat tagtacagat tttggggcta cccgttttgt taatggtgaa ttacaaagca
      481 gacattctgg tatcgatatt gctgctgata aaggaactcc cgtcttggct gccaatggag
      541 gaattgtaaa actggcagcg catttactgg taaccggtaa taccataatt attgaccacg
      601 gttttaatat attttcttca tattcacacc tttctaagct tgatgtaaat aaaggagaac
      661 aagttaaaaa gggtgaaaag attggtgaaa tcggctccac cggtttttct accgggccac
      721 acctgcactg gacaattaca gtgggcaggg tttttgtcaa tcctagaaat ttcgtagaaa
      781 aagatattct aaaatgaaat taatccatat acagtaaaag aagggaaata atttctcatt
      841 atttcccttc ttcctcaaaa aatctatcaa ggagtcatat tatttagata atatacaacc
      901 gcccaaatca caactattaa accaatgatt tctaacaaaa atatcactcc cttctcgaca
      961 attcttttta tttttgagat caaattaacc atttgattta ctacccttaa ttttaccata
     1021 ttatttcaac attaaaaagt taacaggtaa catttactta tctatctttt caactgcatt
     1081 tgctccacat tctggacaaa ttttaccttg gtttctccgc ccatgtccct tacgatgctt
     1141 attaatcttt aacatgaatt ctgctccaca ctctttacat ctatatcgac ctttatccag
     1201 ctcataatct ccgccctcaa atcttaaagc tttaccttct aaaagagctt cagccacttt
     1261 ttgatgggct tttgttataa ttctctggaa ggtaggacgc gaaatattca ttttctcagc
     1321 actttccgtt tgatttaagc cctctacatc tttaagcctt aaagcttcaa cttcttctag
     1381 acttaataaa acttcttcta attcccggcc aggaatccca gctggtttaa aaaatcttat
     1441 gtagggaatg ttcttaattc ttcgtctttt gggtggccta ggcatttaat cactccttac
     1501 taacttatta catatatttt atcattaatt tagtactatt taaagaaaat tcgtttaatc
     1561 cccgtcatca cgcttaataa ttagggtata ttctccttca aaaggcctta actccccctg
     1621 aaaaagatct gttaaccagt tcatagtatt agggaaagga ccttcaccct tatattcagg
     1681 tagtaattca cgcattttca aagccagtct ttcggcaaaa tcttgatcac tttcaccttt
     1741 tttacaggta gataactccc ctatctttat ctttatcgga ttgcggaagg ataatttttt
     1801 tgtgccggaa attgccaccg gcaggatggg cacatctaac tttttagcca cagaaaaaac
     1861 ccctagataa aaagggaaaa tttgtccttc ggcatgtcct aatctcccct ccggaaaaac
     1921 ggcaagatta ttaccttcct ttattatcct catcatggaa cgaacagctt taatgcggga
     1981 cttcgttata tccctattta taggtacaat cggtttcccc atcacatcaa taaatttttt
     2041 cttttttgca ttatcatata ttccttcatt ttctgctatg aatataattt taggggcaga
     2101 gggaaagatc attaaaataa agaaggggtc aatccaattt agatgattag aaattataat
     2161 ataatttctg tcctttggta tattgttttt ccccttaata tctaaattaa agaccgttct
     2221 aaataagcct ttggcaaaaa atcttgttat ataaaatggt actggtggtt tagaaataga
     2281 aatcacctct taaagtacat atatttaacc tatattatat aattcaatat tattttttaa
     2341 tcaccttttt aataatcttc ttgatatatt ttagccaagt taatataata aaatcaactg
     2401 aaataaaggt ggcaaaagat gtcaaaaaaa gatgttaaac taaaaaatat aatttcatat
     2461 ggtttaggag atatatatgg gggcggcgct tttttacttt taggaaccct ttatatgatc
     2521 tttctaaccg atatagtcgg tttaaacccc gctttagccg gttcagtcgt tgtgataggt
     2581 aaagtatggg atgcaatttc agatccttta atgggctata tttcggacaa tatagacacc
     2641 aaatatggac gtagaagaat attttttctt atcggcatta taccgatcac tataagtttt
     2701 atttcactct ggatacctct taacactaat tccagtcttc ttttatttgc ttactattca
     2761 tttgcctata ttttcttttc aaccgtcttt acgatggtca tggtccctta ttctgccctt
     2821 aatgctgaac tgtccgaaaa ctttgccgta agaaccaaac tttctaccgc tcggatggcc
     2881 ttttctcagt tttcagcttt gatttcaggt gttttaccta aatatttgat cgatttttta
     2941 aataaaggta cgattgtttc gcctcattac agttatttaa tcatggggat tgtatttggt
     3001 gttttatatg ccctcccctg gcttttcgtt tttaaaggaa cctgggaaat accttatgaa
     3061 gtagataaag aaacaaaatt catagaaaag attaaaagat ataagacggt tctatataat
     3121 aagtctttta aaatacatct cggtatgtat atttctgctt atcttgccat ggactttttg
     3181 atggccttat tcatctatta tctcacctac tatatacaaa aaccggatat ctttcccttc
     3241 tgtatgttgg caatattatt aagtcaaatt gtaatgctgc ccgtatacat gaaaattagt
     3301 aatacgaaag gaaaaagctt agcctttacc atcggtttaa ctatttgggg aattggtatg
     3361 ttcaacttgc tgttcttatc tgccagtact cccctctata ttataataat aatctgtatt
     3421 gtaatcggtt caggcttatc agccggagta atgataccct gggctatttt acccacaatt
     3481 tcagatattg accaggtaat taatatcaag agaaggaccg gtatctattc gggtttgatg
     3541 acctttattc gcaagattgc ccaggcagtt gccttatggt tagtaggtat tatcttaagt
     3601 ctgatcaact atgtaccaaa ccaaccccag tcaccaacta ccctttttta tcttaagtat
     3661 atatttgtct ttgtcccgat tatcttaatc ggacttggca tattttttag ttataaatat
     3721 aaggttaccc cccgcaagca aaagatcttg attgaagaaa ttaaacggat caaaaacggg
     3781 ggcgagaagg ctggagcaga ttcagaagta aaaaaagttt gtgaagaact caccggtgtt
     3841 aaatacagat aaagcagagg attaactaaa tcccctgctt tttttctttt taatattctt
     3901 ttatctcatc cagtttttct tacacttgaa tttcgcccga agctacttca ttagcaatac
     3961 tttctatcca tttattggtc tcatcgctga cattattgac cggtagtttt aaatacctac
     4021 cctggtctgg tccatattcc atattgtaaa gtccagtcgt attatcttca tttattatac
     4081 catttactat ttcaataatt agactattat atttaaatag atctgaggtt aaaaa
//
LOCUS       DATGCJ010000186        12804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_160630, whole genome shotgun sequence.
ACCESSION   DATGCJ010000186 DATGCJ010000000
VERSION     DATGCJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160630"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VKN64_08710"
     CDS             complement(<1..609)
                     /locus_tag="VKN64_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMA60358.1"
                     /translation="MLLNFFMYLIGLVLIYLIIIIFSPVFPKPRQPIESNNEFKAADS
                     LPNRIDISCKVEEDTIRGWLYLPDDISGKVPCVILNNGFGGTKDMILENYAKEFIKEG
                     IAALSFDYRFFGESDGQPRQMFSVNRQIEDLLAAVNYVKNREEIDKNNIAIWGTSAAG
                     GYGIIAAAKDKDIKCIIGQCPGLDNNADNNDVFKREGIGFFIK"
     gene            complement(726..1742)
                     /locus_tag="VKN64_08715"
     CDS             complement(726..1742)
                     /locus_tag="VKN64_08715"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HMA60359.1"
                     /translation="MTNITYFKGTREEVGFQIGQSMGDRLEKIINNMVESMDNRFGIN
                     RAKLQKEAMTWFYSIPEDYQKELQGMSKGSGVDLKKIADWYYSDKCIKNGCTSFFIKV
                     NNNYWAGRNNDYILPKFWDHINIIKVEDKIPVMLFGLKGDIFTGTGYNKEKLWIHYNW
                     LPIWDKPDNTDNVLEPFIFIRKALEESENLKDVEDLINTTKRTGGMNLFVLDGKKEEC
                     AVFECTGQTYIKRLPKGDYIIGANHYNSLEVPGEYSFDFNNSNKRQKRVKELIEKDDF
                     KKAPQDLIDILSDKGVEQNTKRSKTVYANVACPAQDLIWFAYDGFPAASQSSWEKLRW
                     DWNF"
     gene            complement(1808..2323)
                     /locus_tag="VKN64_08720"
     CDS             complement(1808..2323)
                     /locus_tag="VKN64_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA60360.1"
                     /translation="MNYNKIHIIGGPGSGKTYCAERLSQKLNIKNYDLDNIFWDKTVN
                     KYGIKNSKDKRQEELAKILEQGSYILEGVYYDWLEESFKKADLIIVLKTSVWIRDYRI
                     IRRFIKRKLGTIESKKETFKDFYNLIKWNHRYDKRNLKDALRLIKEHNNNIIFASSYQ
                     ELLEKIFKNYD"
     gene            complement(2349..2762)
                     /locus_tag="VKN64_08725"
     CDS             complement(2349..2762)
                     /locus_tag="VKN64_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA60361.1"
                     /translation="MNKTKLFACAIVLITNDIEKTVEFYKEELGFEVVKHYEQEEKFA
                     ACYRDEVEIVVVKAKKGNIVPNNERYGAGYDAYFVPEDVDLFYQELKNKDVKIIQRPV
                     LTAYGSKEFKIEDIDSRIIGIGKIQDYDKFFKRNS"
     gene            complement(2863..3597)
                     /locus_tag="VKN64_08730"
     CDS             complement(2863..3597)
                     /locus_tag="VKN64_08730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60362.1"
                     /translation="MKYEVIKNIPHHKEIQYEITKELWPEFMFHDPISNKYWDKLFEL
                     FSEFQFSLKSKGEIIAIGQCLPLYWGKPFEDLPEEGWEWALQKGIEDNFKGIEPNVLT
                     GLQIAVNKKHQGKGISSLVLKEMAAIAKEKGLENVIIPIRPSLKSLYPLIDIEDYINW
                     ERDDGTPYDPWIRVHKRFGGEIIKVCHRAMKITGTIADWERWTNLKFLQDDQYVVKGA
                     LKPINIDLDNDLGSYIEPNIWVLHRL"
     gene            complement(3630..4538)
                     /locus_tag="VKN64_08735"
     CDS             complement(3630..4538)
                     /locus_tag="VKN64_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMA60363.1"
                     /translation="MRKAFKISLLIIVIFAILIFSGITYFTNNILSEMAETRVEMDSE
                     LVDKYGLMIDSIEVTTSDGLNINAWRFSQKDPEAIAIVIHGMNGQDGASLLNFGKFFN
                     DQNFAAFCLDLRAHGRSEGNEIGFGYTETRDVKALLDWINKQKKYEGKDIFLYGISMG
                     GATAINSAAKFESIDMVISVSSFASHEATFLDYMRREEIPEIIVQSFKPSIRLLLALK
                     YNTKPIANAPINTIKNVEDPIFIIHGQQDEQILVHQARELKKAAGENAKLWIVDDKKH
                     MVVTEIFKEENQWYRDRLLEYINNNI"
     gene            complement(4554..5252)
                     /locus_tag="VKN64_08740"
     CDS             complement(4554..5252)
                     /locus_tag="VKN64_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HMA60364.1"
                     /translation="MKTIGLIGGMSWESSLEYYRIINQIVKEKLGEPHSAKCIMYSVD
                     FAEYEKLQHEGKWEELTEKMVDIGRRLEAAGSDLILICTNTMHKMAPQVQESIKVPLL
                     HIADAAADEIKSRNMGKVGLLGTKFTMEQDFYKKRIKDNYGIDVIIPKQEERDIVHKV
                     IYKELISGIINESSRKKFQKIIENLKERGAEGVILGCTEIPLLIKEKDSVIPVFDTTM
                     LHARKAVNLALKDK"
     gene            complement(5252..5962)
                     /locus_tag="VKN64_08745"
     CDS             complement(5252..5962)
                     /locus_tag="VKN64_08745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HMA60365.1"
                     /translation="MTYIYLIALLLSHIIIMRAIKNKIVKKKIHKVFKIKVIMAPLSV
                     VLSFLLIRVIELPLLEAGLTMGNSSAGIRSVMYIGLPLALISSALVFTTPKEELDQVK
                     YGNQKDIWTFIYVWVLVGPVEELLYRGFVQGTLSTLIKGKLFYFSYATIIASVIFVLA
                     HVTNVYTKNESWRSFFSMVPTRFIAAMVLGFSFQVSDSLLYPIIIHNLIDGFNLSALT
                     YRKQKLKSENIDNYKEES"
     gene            complement(5989..6501)
                     /locus_tag="VKN64_08750"
     CDS             complement(5989..6501)
                     /locus_tag="VKN64_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60366.1"
                     /translation="MDCKIRWAQEKDAQALGELHTASWQAAYKGIIPTEVLNNLDAER
                     RTQKFKEAIKENKEETAVLLVEDKVVGFITLGKNRDEDCGPGTGEIWGIYLHPEYYNQ
                     GLGSELIQWGIKKLKKRGYKKITLWVLKDNESSRKFYEKKNFIFDGTVGEIKKGKTIK
                     KVRYVYNIYD"
     gene            complement(6558..7472)
                     /locus_tag="VKN64_08755"
     CDS             complement(6558..7472)
                     /locus_tag="VKN64_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60367.1"
                     /translation="MEVKKVHKKEIKEFTSLNKNSAQFEKAVKNLWNNGLSFPNWCFT
                     IAQEGKIVGRVGYWAPADNHKEVRIFGLSLPWQSDNLFQIGQELLKESFKTMQDYGVE
                     YIDSQLDSSEEDIFVLSQKLYKHVGLNQIQAKKRYKLDISEYDYTGNNRLNYRPLKEM
                     GEDYFIDIIKEVTKGTFDKEDKLNIEKDGEYEAAKKHYCSLKSIDYSPDNWFIAYHNK
                     ELVGLVIPQHLNGDLGTINYIGVIPAKRGNGYVTDLLDKGIKNLIKRDINEIIADIDI
                     ENYPMEEALLKMGFTKEKELLNYRKELN"
     gene            complement(7503..8435)
                     /locus_tag="VKN64_08760"
     CDS             complement(7503..8435)
                     /locus_tag="VKN64_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA60368.1"
                     /translation="MPKDLFSKNNWKKLKAPLIFFFIFLGLGIWRYQVTDKIFYFYNF
                     TYIGAALGIGIYLNSILAKENKQLGRKITLLLIGIYMLGYLGIIRKENMQLEGFFFYL
                     LAGIFAAATLHYLIAKIVGPLIFGRGWCGWACWTAMVLDFLPWDNNQRRRKNLSKLRY
                     IHFILSFIIIIYLWFNNGNIFSDLNLALYWLAAGNIIYYAIGIGLAYLLKDRRAFCKY
                     ICPIPTLQKITARFSLLKIEVEPDKCIECGLCEEHCPMGIKILKYKNEGKRVLSSECI
                     FCQNCQDICPQEAVDVTVGLDGQYNKEYLYYDQS"
     gene            complement(8526..9593)
                     /locus_tag="VKN64_08765"
     CDS             complement(8526..9593)
                     /locus_tag="VKN64_08765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMA60369.1"
                     /translation="MNKDKVKRSIEEMFRKQVQKDSKVNNAYLLVHSDKLDIHLDIAE
                     GKTGELSANPKQPNYMASVGKLFTSTLISILYEKGELSFEDKIAKYLGDEILDGLHVY
                     KGQDYSKEIKIKHLLKQTSGLYDYFWPLLEKVLADKDFHISTREAIEWGKNNLKPCCP
                     PGKKVNYTDTNYHLLGFIIENITGKPFHEVLEEYIFTPLNMEQSSMLFTSEPIREYEY
                     PIAHFSIDGTIGNEFKNYVDLDYAGGGVVATNQDLLKFMKALVAGEIISKDTLETMKN
                     ETDKLFIGIDYGYGIWKFKSIPILMPKKLKAWGCSGATGAFLFYHPGTDSYVIGNFND
                     VSYKSKGLRFMMKVIKKLTKV"
     gene            complement(9697..10746)
                     /locus_tag="VKN64_08770"
     CDS             complement(9697..10746)
                     /locus_tag="VKN64_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60370.1"
                     /translation="MAEDIKVKKAVNWYKNNRERYKELANNVENIIKEMLIEKDIYYH
                     SIASRAKQVDSFQRKAKREKYTEPLKEITDLAGIRIITLFEKDVYEISDLIKEVFIID
                     YENSEDKADLLDADKMGYKSVHYIANVTKELVDAELYEQFKDLKFEIQIRSILQHAWA
                     EIEHDRNYKLKGNLPKHLQRRFYALSGMLEMADREFNLLAEEVDEYKKKVKELTRNGE
                     IDIEIKPTSLQKYLLSKFREEVNDGLLEPTFDNQAAEIIKELSNYGIWDLVNLDSIIP
                     ENINEEIKDNLLDEESLDFNSLLKIIMIVNDYHKYFEKVWNQKSNFLNEKIIEIFKDY
                     DIPIKKLAEEYNFKI"
     gene            complement(10762..11181)
                     /locus_tag="VKN64_08775"
     CDS             complement(10762..11181)
                     /locus_tag="VKN64_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60371.1"
                     /translation="MIEFVKSMVRDSNPAEVAENVVNDFFEKKKMNTFMVEVEEKRIG
                     FSVLKEEPFEGAPNVAEIVWLKVDEPYQRKGYGNRAVKYLEKFASEKDIRKVYVKTSS
                     KNKQAVCFWIKAGYKFEARLLDFSYEGHDDYFLTKKL"
     gene            complement(11247..11774)
                     /locus_tag="VKN64_08780"
     CDS             complement(11247..11774)
                     /locus_tag="VKN64_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HMA60372.1"
                     /translation="MTVKADRSILILLPDKKIEGIEDFRKQHIQSEGKEVPFHITLLP
                     DFYLPNELDDNIISKLKLISNTFPKFDFKAVPISSFPTSKVIYLSPVPAGSIEQLVYK
                     IKVLLPRYKDKERSAHIYHMTLAMGNPLDQTNEIIEDYFNKFDKDPLLLKGGKLAIYI
                     KEQGKWSEYQSFELS"
     gene            complement(11801..12298)
                     /locus_tag="VKN64_08785"
     CDS             complement(11801..12298)
                     /locus_tag="VKN64_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60373.1"
                     /translation="MKIKLKEAKKEDLDFLAKVCRDAKDAYSEIMPEAFEKQAKEFEE
                     HGLPEDYKIYVVKDDNKKIGFVGITDLTEQTAYLVGLYLLNDYQREGYGTATIDEVIK
                     TLKDKDYNKICLLVHNEAEWAIDFYKKEKFVSLTECEDIIKNYSGGKMEDYYLPSTIF
                     MEKNF"
     gene            complement(12328..>12804)
                     /locus_tag="VKN64_08790"
     CDS             complement(12328..>12804)
                     /locus_tag="VKN64_08790"
                     /inference="COORDINATES: protein motif:HMM:NF040149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HMA60374.1"
                     /translation="YSCLSKPAQKVIEWTNAFGEGFAMLAAAKGLENHPNKFDKDLKS
                     KWDKNMENFNKDFLKIQNFLLAILEGHFADDQKLYNRGFQLMTNNDGQGSWYTVGYQI
                     AVIIEREAGREVLIECMKDLTKIYFQYNNLVEIYNKKRNEDLPKWNDKILTSLKRK"
BASE COUNT         3804 a         2519 c         1618 g         4863 t
ORIGIN      
        1 ttttataaaa aatccgatcc cttctctttt gaaaacatca ttattatcag cattattatc
       61 aagacccgga cattgaccga taatacattt tatatcttta tccttggcag ccgctattat
      121 accataacca ccggcagctg atgtacccca aattgcaatg ttatttttat ctatttcttc
      181 tctattctta acataattta cagctgctaa taagtcttca atttgtctat tcactgaaaa
      241 catctgtcgg ggttggccat cactctcccc aaaaaatcta taatcaaatg ataaggctgc
      301 aattccttct tttataaatt ctttcgcata gttctctaag atcatatctt tagttccacc
      361 aaatccatta tttaatatta cacaaggaac ttttccagaa atatcatctg gtagataaag
      421 ccaccctctt attgtatctt cctcaacttt gcaactgata tcaattctat tgggtaaact
      481 atccgctgct ttaaattcat tattgctttc aatgggttgt ctaggtttag gaaatacagg
      541 agaaaatatt attataatta ggtagatcaa aaccaaacca atcaaataca tgaaaaagtt
      601 taaaagcatt ttatctccta ctttctaaca tatattttca tctaatatac gtattatttc
      661 ctatctatat tatatcatga attgattcta aatttttctt tttttattag aaatgataaa
      721 ttcatctaaa agttccaatc ccatcttagc ttttcccagc tgctttggct ggcggccgga
      781 aaaccatcat aggcaaacca gattaaatct tgagctggac aagctacatt agcataaact
      841 gtctttgacc tttttgtgtt ctgctctact cctttatcag acaagatatc tattaagtcc
      901 tgaggtgcct ttttaaaatc atctttttca attaattcct taactctctt ttgcctttta
      961 tttgaattat taaaatcaaa actatattcc cctggtactt caagagaatt ataatggttt
     1021 gcgcctatta tgtaatctcc ttttggcaat ctttttatat aagtttgtcc agtacattca
     1081 aatactgcac attcttcttt tttgccgtcc agaacaaata gattcattcc acctgttctt
     1141 ttagtagtat ttataagatc ttctacatct tttaaatttt cactttcttc tagggctttt
     1201 ctgatgaaaa taaacggttc taacacatta tctgtattat caggcttatc ccaaatgggc
     1261 agccagttat aatggatcca tagcttttct ttattatagc cagtaccggt gaaaatatct
     1321 cctttaaggc caaataacat gacaggtatc ttatcttcta ctttgattat atttatatga
     1381 tcccagaatt ttggcagtat ataatcattg tttctacccg cccagtaatt attattaact
     1441 ttaatgaaaa aactggtgca gccattttta atacatttat cactataata ccagtcagcg
     1501 atctttttta agtcaacacc cgatcctttt gacatacctt gaagttcttt ttggtaatct
     1561 tcaggaatgc tataaaacca ggtcattgct tctttttgta atttggctct attaatccca
     1621 aaccggttat ccatactctc aaccatatta tttatgatct tttctaatcg gtcgcccatg
     1681 gattgaccaa tttgaaatcc cacttcttct cttgtgccct tgaaataagt aatatttgtc
     1741 atcactatac ctctttttta ataattgata gtatttttat tattaaacaa caatatttca
     1801 gaacttttta atcataattt ttaaatattt tttcaaggag ttcttgataa gaacttgcaa
     1861 aaattatatt gttgttatgt tcttttatta accgtaaggc atccttcaaa ttccttttat
     1921 catatctatg gttccattta attagattat agaaatcttt aaaggtttct ttttttgatt
     1981 ctatagttcc cagtttccgt ttaataaatc ttcttattat tctatagtcc cttatccaga
     2041 ctgaagtttt taatacaatt attaaatcag cttttttaaa actttcctca agccagtcat
     2101 aatatactcc ttctaatatg taactacctt gttctagaat tttagctaac tcttcctgcc
     2161 ttttatcttt tgaattttta attccatact tattaactgt tttatcccaa aaaatattat
     2221 ccagatcata atttttaata tttaattttt gagataatct ttcagcacaa tatgtcttac
     2281 cgctgccggg tcccccgatg atatgtatct tattataatt caagtttaag ataactcctt
     2341 tttatgaatt acgaatttct tttgaaaaat ttatcataat cctgaatctt accaatacct
     2401 atgatcctgc tgtcaatatc ctcaatttta aattctttgc tgccataggc agttaacact
     2461 ggtctttgaa taattttcac atctttgttt tttaactctt gataaaaaag atcaacatct
     2521 tccggtacga aataagcatc atagccggct ccatatctct cattattggg tactatatta
     2581 ccttttttag ccttaacaac aactatttct acctcatccc gataacaggc tgcaaatttt
     2641 tcttcttgtt cgtaatgttt tactacttca aaacccagtt cttctttata aaattctaca
     2701 gttttttcaa tatcattagt tattaataca attgcacagg caaaaagttt tgttttattc
     2761 aatattatca tccctattta tcaatagatt gttttttcaa ttttcccaaa cgataattga
     2821 ttattcaata ttcttaaatt aatatttaac tgcaaaaatt tattataatc gatgtaaaac
     2881 ccaaatatta ggttcaatat aacttcctaa gtcattatca agatctatat ttatcggttt
     2941 caaggcaccc tttacaacat actggtcatc ttgcaggaac tttaagttag tccatctctc
     3001 ccaatcagct atggtacccg ttattttcat tgccctatga caaaccttaa ttatttctcc
     3061 tccaaaacgc ttatgaaccc tgatccaggg atcatagggt gtaccatcat ctctttccca
     3121 attaatataa tcctcaatat caattaaggg ataaaggctt tttaaactgg gtctaattgg
     3181 gattattaca ttttctaaac ccttttcttt ggcaatagcg gccatctctt ttaatactaa
     3241 tgaacttata cctttaccct gatgtttctt attaacagca atttgcaggc cagttaaaac
     3301 attaggttca atacctttga aattatcttc aatacctttt tgtaaggccc actcccaacc
     3361 ttcttccggt aagtcttcaa atggtttacc ccagtataag ggtagacact ggccgatcgc
     3421 aattatttct ccttttgact taagagaaaa ctggaattct gaaaataact caaaaagttt
     3481 atcccagtac ttattggaaa tagggtcatg aaacataaac tcgggccaga gttcttttgt
     3541 tatttcatac tgtatttctt tatgatgtgg aatgtttttt attacttcat atttcattta
     3601 gataactcct tttttaaaaa aagaatacct taaatattat tattgatata ttctaataac
     3661 ctatctctat accattggtt ttcttccttg aaaatttctg taactaccat gtgcttttta
     3721 tcatcaacaa tccataactt cgcattttca ccagctgctt tttttaactc tcttgcctgg
     3781 tgaacaagaa tctgttcatc ctgttgcccg tggattatga aaatcggatc ttcaacattt
     3841 ttaatggtat ttatgggagc atttgctata ggtttagtat tatattttaa agctaatagg
     3901 agccttatac tgggcttgaa agactgtact ataatttcgg gtatctcctc tcttctcatg
     3961 taatccaaaa aggtagcttc atgggaagca aaagaactca cactgatcac catatcaata
     4021 ctttcaaatt tggcagccga gtttatggcg gttgccccac ccatagaaat gccgtaaagg
     4081 aatatatctt ttccctcata ctttttctgt ttatttatcc agtctaataa ggccttaaca
     4141 tctcttgttt cagtataacc aaagcctatc tcatttcctt cacttctgcc gtgggctctt
     4201 aaatccaggc aaaaagctgc aaaattttgg tcattaaaaa acttaccgaa atttaaaaga
     4261 gacgctccgt cctggccgtt cataccatgt ataacaatag cgattgcttc cggatctttt
     4321 tgggaaaacc gccaggcatt aatattcaaa ccatcagatg tagttacctc aatactatct
     4381 atcattaaac catacttatc taccaactca ctatccatct ctaccctggt ttctgccatt
     4441 tcagagagta tattattggt aaaatatgtt ataccactga atattaaaat agcaaagatt
     4501 actattataa gcagactaat tttaaatgcc ttcctcactc cacagacact cctttattta
     4561 tcttttaaag ctaagttaac tgcttttctc gcatgtaaca ttgttgtatc aaaaacggga
     4621 attacactgt ctttttcttt tattaataag ggaatttctg tacagcccaa tataacaccc
     4681 tcggctcctc tttcctttaa gttttcaata atcttttgaa atttctttct agaagattca
     4741 ttgataattc cagagatgag ttctttgtag attactttat ggactatatc tctttcttct
     4801 tgtttaggaa tgattacatc tatgccgtaa ttatctttaa tccgtttttt ataaaagtcc
     4861 tgttccatcg taaatttggt acctaataaa cccacttttc ccatgtttct acttttgatc
     4921 tcatcagcgg ctgcatctgc aatatgtaaa agtgggactt tgatactttc ctgtacctgg
     4981 ggagccattt tatgcatggt attagtacag attagaatta aatcagagcc cgccgcttct
     5041 aatctccgac ctatatcaac cattttctcg gtcagttctt cccatttccc ttcatgctgc
     5101 agtttctcat attcagcaaa gtccacggaa tacatgatac acttagcaga atggggctca
     5161 cccaactttt ctttgactat ctgattgatt atcctgtagt attctaaaga tgattcccag
     5221 ctcattccac cgattaatcc aattgttttc attagctctc ctccttgtag ttatctatat
     5281 tttcactttt taatttctgt tttctataag ttaaagcact taaattaaaa ccatcaatta
     5341 ggttatggat aatgattgga tacaacaaac tatctgagac ttgaaaacta aagcctaaca
     5401 ccatggctgc tataaatcta gttggtacca tactgaaaaa agatctccag ctttcatttt
     5461 tagtatagac attggtaaca tgggccaaaa caaaaattac acttgcgatt attgtagcat
     5521 aggaaaaata aaataatttt cctttaatta gtgtacttaa tgtaccctgg acaaatcccc
     5581 tgtataataa ctcttctacc ggtcctacta aaacccatac ataaataaaa gtccagatat
     5641 ctttttgatt tccatacttt acttgatcta attcctcttt gggagtagtg aataccaaag
     5701 cagaactaat taatgctagt ggtaaaccta tatacatgac tgaccgtatg ccagcactac
     5761 tattacccat ggtaaggcca gcctctaaca gaggtaattc aattactctt attaaaagaa
     5821 atgaaagaac cacacttaat ggggccatta taaccttgat tttaaaaact ttatggatct
     5881 ttttctttac tatcttattt tttattgccc tcattattat tatatgagat aagagaagtg
     5941 cgataagata aatatatgtc atgttctcat cctttctgag tttaaaaatt aatcataaat
     6001 attatataca tatctaacct ttttgatcgt tttccctttt tttatctctc ctactgttcc
     6061 atcgaatatg aagtttttct tttcgtagaa ttttcgtgaa ctttcattat cttttaatac
     6121 ccataaggtg atctttttat atcctctctt ttttaacttt tttatgcccc attgaataag
     6181 ttcactgccc agcccctggt tataatactc aggatgaagg tagatccccc aaatttcgcc
     6241 tgtaccagga ccgcagtcct catctcgatt tttgcctaat gtaataaagc cgactacttt
     6301 atcttcaact aataataccg cagtctcttc tttattttct ttaattgctt ctttaaattt
     6361 ttgcgttctt ctttcagcat ctaagttatt taaaacttca gttggtatta ttcctttata
     6421 agctgcctgc cagcttgcag tatgaagttc tcctaatgct tgagcgtctt tttcttgggc
     6481 ccaacgtatt ttgcagtcca atctaaacac ctcaaaaaat atttataatt ttgtaaaatt
     6541 ttacctaaaa taaaaagtta atttaactct ttccgataat tcaataactc cttttcttta
     6601 gtaaatccca tctttaaaag ggcttcctcc atgggataat tttcaatatc aatatcagct
     6661 ataatttcat ttatatctct ttttattaaa ttcttaatac ccttatctaa aagatcagtt
     6721 acataaccgt tacctctttt agcaggtatc acaccgatat aattaattgt ccctagatct
     6781 ccatttaagt gttgcggaat aacaagacct actagctctt tattatgata ggctataaac
     6841 cagttatcag gagagtaatc tatacttttt aacgagcagt agtgtttttt ggctgcttca
     6901 tactcaccat ctttttctat atttaattta tcttctttat caaaggttcc cttggtcact
     6961 tccttaatta tatctataaa ataatcttct cccatctctt ttaatggtct ataatttaat
     7021 ctattgttac cggtataatc atattcagat atgtctaact tgtatctctt ttttgcttgg
     7081 atttggttaa gtccaacatg tttatataat ttttgactta aaacaaatat atcttcttca
     7141 gaagaatcaa gttgtgaatc aatatattct acaccataat cctgcatggt cttaaagctt
     7201 tcttttaata attcttgccc aatttggaat aagttatcac tctgccaggg cagggataat
     7261 ccaaagattc taacttcttt atggttatca gcgggtgccc agtatcccac tctaccgaca
     7321 attttaccct cctgtgcaat cgtaaaacac cagttaggaa aggataaacc gttattccag
     7381 agatttttga cagccttttc aaattgggcc gagtttttat tcaaactcgt gaattccttt
     7441 atctcttttt tatgtacctt tttcacttcc atactttcgc ctactttcat aaatatatat
     7501 atttatgatt gatcataata taaatattct ttattatact gtccatctaa accaactgtc
     7561 acatccactg cttcttgcgg acagatatct tgacagtttt gacaaaatat acattcggat
     7621 gataataccc ttttaccttc atttttatac ttcaatattt taatacccat cgggcagtgt
     7681 tcttcacaca gaccgcattc tatacattta tcaggttcaa cttctatctt aaggagagaa
     7741 aatcgcgccg ttattttctg cagagttgga attggacaga tatacttgca gaaagctcgc
     7801 ctatctttta gtaaataggc taaacctata cctattgcgt aatatattat attccctgca
     7861 gccagccaat aaagtgcgag attcaaatcg gaaaagatgt tgccgttatt aaaccaaaga
     7921 tatattataa taataaatga taggataaaa tgaatatatc ttaacttact taggtttttt
     7981 cttcttcttt gattgttatc ccagggtaaa aaatctaata ccatggccgt ccagcaggcc
     8041 cagccacacc aaccgcgccc aaatattaag ggaccaacaa ttttagcaat aaggtaatga
     8101 agagttgcgg cagcaaaaat accagccaat agataaaaga aaaaaccttc taactgcatg
     8161 ttctcttttc ttattatacc tagataaccc agcatataga ttccgatcag cagcagagtt
     8221 attttccttc ccaactgttt attctcttta gctaagatag aatttaaata tattcctatt
     8281 cctaaagcgg ctccaatata ggtaaagtta taaaagtaga atattttatc agtcacctga
     8341 tatctccaaa ttcctaaacc taaaaatata aaaaagaaga tcaaaggtgc ctttaatttt
     8401 ttccaattat tcttagaaaa caaatctttg ggcaaactat ccccccgaac attcatacag
     8461 gcttactttt taatttaaat tcaaaactat ttttttttac tctatgaata ttatattttt
     8521 ttattttata ccttagttaa ttttttaatc actttcatca tgaatctaag ccctttactt
     8581 ttataggata catcattaaa attacctatt acataagagt cagtacctgg atggtaaaac
     8641 aaaaaagcgc cggtggcccc tgaacatccc caggccttta gttttttggg catcaggatg
     8701 ggaattgact tgaatttcca gatcccataa ccataatcta taccgataaa tagtttatct
     8761 gtttcatttt tcattgtctc cagggtatct ttagaaataa tttcaccggc taccaaagct
     8821 ttcatgaatt tgagtaaatc ctgattggta gctactactc ctcctccagc ataatctaaa
     8881 tctacataat ttttaaattc attgccaatt gtaccatcaa tactaaaatg ggcaattgga
     8941 tattcatatt ctcttattgg ctcagaggta aagagcatcg aggattgttc catatttagg
     9001 ggagtgaaaa tatattcttc taatacctcg tggaacggct tacctgtaat attctcgata
     9061 ataaatccta aaagatggta attcgtatct gtataattta cctttttacc cggtggacag
     9121 cagggcttta aattattttt tccccattca attgcttccc gggtacttat atgaaagtct
     9181 ttatctgcca gtactttttc taaaagaggc cagaaataat catataaacc agaggtctgt
     9241 tttaagagat gcttaatttt tatctctttt gaataatcct gccccttata gacatgcaga
     9301 ccgtccaata tttcatcacc taaatatttg gctattttat cctcgaagga tagttcgcct
     9361 ttttcgtata agatgcttat caaggtagag gtaaataatt tacccacact ggccatataa
     9421 tttggctgtt ttggattggc cgacagctct ccagtcttgc cctctgcaat atctaaatgt
     9481 atgtccaact tatcagaatg gaccagcaaa taggcattat ttactttaga atctttctgc
     9541 acctgttttc taaacatctc ttcaattgat ctttttactt tatccttgtt cacttcttca
     9601 ctttcctttc atattatatt ttttaaaaaa ttaattaatc ttttaaaaac taatttataa
     9661 ttgagagact taagttgctt atattattta attgatctat atcttaaaat tatactcttc
     9721 agctaacttt ttaatgggaa tatcataatc cttaaagatt tcaataattt tttcatttag
     9781 gaaattactc ttttgattcc agactttttc aaaatattta tggtagtcat ttacaatcat
     9841 tatgattttt aataaggaat taaagtctaa actttcttca tccaacagat tatcttttat
     9901 ttcttcattt atattctctg ggattataga atcaaggttt accaaatccc agatcccgta
     9961 attagacaat tctttaatta tttcggctgc ctggttatca aaagtaggtt ctaataatcc
    10021 atcattaact tcttccctga attttgataa gaggtacttc tgtaaagaag ttggcttaat
    10081 ttcaatatct atttccccgt tgcgggttag ttctttcact ttctttttat actcatcaac
    10141 ttcctctgcc aggagattga actctctatc tgccatctct aacatgcctg ataaggcata
    10201 gaaccggcgc tgtaagtgtt tcggcaggtt accttttagt ttataatttc tatcatgttc
    10261 tatttctgcc caggcatgct gtaagatact cctaatctgt atttcaaatt tgaggtcttt
    10321 gaactgctca taaagttctg cgtccactaa ttcttttgtt acatttgcta tataatgtac
    10381 ggacttataa cccattttat ctgcatcaag taaatctgct ttatcttcgc tattctcata
    10441 atctatgatg aagacttcct taattaaatc actgatctca tagacatctt tttcaaaaag
    10501 ggtaataatt ctaataccgg ccagatcagt tatttccttc agaggttctg tatatttctc
    10561 tctttttgct tttctttgaa agctgtcaac ttgcttggca cgactagcta tactatgata
    10621 atagatatct ttttcaatta acatttcttt tataatattt tcaacattgt tagccagttc
    10681 tttatatctt tctctattgt ttttatacca atttacagct ttttttactt ttatatcttc
    10741 ggccatctct acccctcact tctataactt tttagttaaa aaataatcat cgtggccttc
    10801 ataactaaaa tcaagcagtc tcgcttcaaa tttatagcca gctttaatcc aaaaacaaac
    10861 tgcctgtttg tttttagagg aagttttgac atatactttt cggatatctt tttctgatgc
    10921 aaatttttct aaatatttaa ctgctctatt tccataacct tttctttgat atggttcatc
    10981 aacttttaac cagacaattt ctgctacatt gggagctcct tcaaaaggtt cttcttttaa
    11041 tacggaaaat ccgattcttt tttcttctac ttccaccata aaggtattca tttttttctt
    11101 ttcaaagaaa tcattaacaa cattctcagc tacttcggct ggattgctat ctctaaccat
    11161 agatttaaca aattcaatca atttattttt atcctttttt attcgcagtt ctaatattga
    11221 ccaaatgtat tcctcctaaa aaaggcttaa cttaattcaa aagattgata ttcactccat
    11281 ttaccctgtt ctttaatata tattgccagt ttaccgccct tgagtagcag gggatcttta
    11341 tcaaatttat tgaagtaatc ttctataatc tcatttgtct gatcgagagg attgcccatg
    11401 gccaatgtca tatggtatat atgggcactt ctctccttat ccttatatct tggtaaaaga
    11461 acttttattt tgtatactag ctgttctatc gatcctgccg gtaccggtga taaatagatc
    11521 accttactgg tgggaaaaga tgaaattggt actgccttaa agtcgaactt aggaaaagta
    11581 ttagatatta attttaattt actaattata ttatcatcca attcatttgg taaatagaaa
    11641 tcaggcagta aagtgatatg gaaaggaact tccttccctt cactttgaat atgctgttta
    11701 cgaaaatctt ctattccctc tattttttta tcgggtaata agatgagaat gcttctgtct
    11761 gcttttactg tcattaatat tttctccctt ttatttttaa ttaaaagttc ttttccataa
    11821 aaattgtaga aggtagataa taatcttcca tcttaccgcc ggaataattt ttaattatat
    11881 cttcacattc tgttaggctg acaaactttt cttttttgta aaaatcgatt gcccattctg
    11941 cttcattatg taccaataga catatcttgt tataatcttt atcctttaaa gtttttatta
    12001 cttcatcaat agttgcagtt ccatatccct cgcgctggta atcatttaag agatatagac
    12061 cgacaagata ggcagtttgt tcagttaaat cagtaatacc aacaaaacca attttcttat
    12121 tatcatcttt tactacgtaa atcttataat cttccggtaa accatgttct tcaaattcct
    12181 tggcctgttt ttcaaatgcc tccggcatta tctcggaata agcatctttc gcatcccgac
    12241 agactttagc taaaaaatcc aaatcctctt ttttagcttc cttcagtttg attttcaact
    12301 tttctccccc cctaattttt ttcatattca ctttcttttt aaagaagtaa gtattttatc
    12361 attccattta ggtaaatctt catttctttt tttattatat atttctacaa gattattata
    12421 ttgaaagtaa attttggtta aatctttcat gcattcaatt aaaacctcgc gaccggcttc
    12481 cctttcaata ataacagcta tttgataacc aacggtgtac caggaaccct ggccgtcatt
    12541 attggtcatt aattgaaagc ctctattata taatttttgg tcatctgcaa aatgtccttc
    12601 taaaatagct aataaaaagt tttgaatttt aagaaagtcc ttattaaaat tttccatatt
    12661 tttatcccat ttacttttta aatctttatc aaacttatta ggatggtttt ctagcccttt
    12721 agcagcagct aacatggcaa agccttcacc aaaggcattt gtccattcta taactttctg
    12781 agctggtttt gatagacagc tata
//
LOCUS       DATGCJ010000187         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_204110, whole genome shotgun sequence.
ACCESSION   DATGCJ010000187 DATGCJ010000000
VERSION     DATGCJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204110"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VKN64_08795"
     CDS             <1..1467
                     /locus_tag="VKN64_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60375.1"
                     /translation="NTDWDTLDPAYCSGFDAGAMAVKFYDGLVRFDYHSNDVVPALAK
                     SWDVEQDNTVFTFHLREGVKFHDGTEFTAHDVKYSFDRLYDPEVASPGTWVYPMIKGT
                     DASLNGETDGLEGVKVLDKYTVQFELDKPFGLFLTHLTLPYGLIVNEDIVDEYGDDFR
                     QHPVGTGPFKFVSWEHDDRLVMEANEDYWDGRPYVDEVVYRVIPQPLTDIAEFEAGNL
                     DLTTVPTEERERWLGNEEWQPYINTMAELSTYYLALNGDFEPLDDPKVKKAIDYAIDS
                     QMVTESLFPHYVAANDAIPEGMPGGADVEFKADVEKAKELLAEAGYPDGFEMELWVSD
                     GSTSVRVGGVLQALLGRVGIEVDLIKNDWSVFYSTVKNGNAPSYYLSWWADYADPYNF
                     LNALYTSDRIVFNNETINSMLDKMASTTNQQERIRLSQEIIKMADQIGDPYVYLYHTS
                     SSSIKQPWIKGEIYHQMYNADKLVTWWIDQDVKAEETN"
     gene            1556..2488
                     /locus_tag="VKN64_08800"
     CDS             1556..2488
                     /locus_tag="VKN64_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60376.1"
                     /translation="MIQYILRRLLLIIPVLLGVLFLTFVLMYMVPGDPVITMLGQHAE
                     DDIINRIRAELKLDDPWHVQFINYLKRTLRGDLGRSYISGVSVTDALKQKLPNTLKLA
                     VTAVIVSTLMGVFIGVISAVYQNTWIDHAAMTFALLFISTPVFWFGMILIYFFSMTLG
                     WTPISGMGDGGLSLQHLILPAITLGSRFAAFLARYTRSVMLEVITQDYIRTARSKGLK
                     ERKVILSHALRNVMIPVVTVVGMNFASLLNGSVLTETIFAWPGFGRYVVTAIKKRDFQ
                     VIAGCVLVGSLIFVFFNLIVDVIYVGLDPRIKYD"
     gene            2509..3396
                     /locus_tag="VKN64_08805"
     CDS             2509..3396
                     /locus_tag="VKN64_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60377.1"
                     /translation="MQANKKDNKTARDKKPKSLWQDTWKRLKRNKAAMIGLVMVILFI
                     LIAIFAPFLAPYDPNEIDLTSVLKAPSADHWLGTDYYGRDLTSRIIYGSRISLSVGII
                     VQTIALTIGTLMGSLAGYYGGKVDMFIMRIVDIVMSFPSLLLTIAIMVALGPSLYNVF
                     LALGVVWWTRTARIIRSEFMRNREKEYVDAARALGNNDFIIMYKHILPNCMAPLIISF
                     TMGIASSIMAEASLSFLGLGAQEPTPSWGSIINNGLQYLRTKPWYSLFPGLAIAYTVL
                     GFNLLGDGLRDALDPRMKK"
     gene            3421..>3672
                     /locus_tag="VKN64_08810"
     CDS             3421..>3672
                     /locus_tag="VKN64_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA60378.1"
                     /translation="MSKEKLIDVSNLKTHFFTEEGVVKAVDGVDFEIYPGETIGIVGE
                     SGCGKSVTSLSIMRLVESPPGRIVDGSIMFEGEDIVKKKQ"
BASE COUNT         1195 a          482 c          757 g         1238 t
ORIGIN      
        1 aatactgatt gggatacact tgacccggca tattgttccg gttttgatgc aggagctatg
       61 gcagttaaat tttatgatgg tttagttaga tttgattatc attctaatga tgtagtacct
      121 gctttagcta aatcatggga tgtagagcaa gataatactg tttttacttt ccatctacgt
      181 gaaggggtta aatttcatga tggtacagaa tttactgctc atgatgtaaa atattcattt
      241 gatagactat atgatccaga agtcgcttca cctggaacct gggtatatcc aatgattaaa
      301 ggaacagacg cttctttaaa tggtgaaact gatggtctgg aaggggtaaa agttctagat
      361 aaatatacag ttcaatttga actagataaa ccttttggat tatttttaac acaccttacc
      421 ttaccttatg gtttaattgt taatgaagat attgtagacg aatatggtga tgattttaga
      481 caacatcctg ttggtacagg tccttttaag tttgttagct gggaacatga tgatcgttta
      541 gttatggaag ctaatgaaga ttattgggat ggaagaccat atgtagatga agtagtgtat
      601 agggttattc ctcagccact tactgatatt gctgaatttg aagccggtaa ccttgattta
      661 actactgttc ctactgaaga aagagaaaga tggttaggta atgaggaatg gcagccatat
      721 attaatacta tggcagaact ttcaacatat tatttggctt taaatggtga ttttgaacca
      781 ttagatgatc caaaagttaa aaaagctatt gattatgcaa tagatagtca aatggtaact
      841 gaatcacttt tcccacatta tgtagctgct aatgatgcaa ttcctgaagg aatgcctggt
      901 ggcgcagatg tagaatttaa agctgatgtt gaaaaagcta aagaattatt ggcagaagcc
      961 ggttatccag atggttttga aatggaactc tgggtaagtg atggtagtac ttctgttaga
     1021 gttggaggag ttttacaggc acttcttggt agagttggca tagaagtaga tcttatcaaa
     1081 aatgactgga gtgtattcta ttctacagtt aaaaatggta atgctccatc ttattaccta
     1141 tcatggtggg cagattatgc tgacccatat aacttcttaa atgcacttta tacaagtgac
     1201 agaatagtat tcaataatga aactattaat tcaatgcttg ataaaatggc atctacaact
     1261 aatcagcagg aaagaattag attatcacag gaaattatta aaatggcaga tcaaattggt
     1321 gacccatatg tatatttata tcacacaagt tcatcatcaa taaagcaacc atggattaaa
     1381 ggtgaaattt atcatcaaat gtataatgct gataaattag taacctggtg gattgatcaa
     1441 gatgtgaaag ctgaagaaac aaattaatta tagcaataaa tatagtaaat aaaaaagatg
     1501 cgtccttttt tataagggcg catctatttt aagaagggaa aaggaggaaa aaattgtgat
     1561 tcaatacata ctaagaagat tactcttaat aattccagta ttgctaggag ttttattttt
     1621 aacctttgtt ttaatgtata tggttcctgg tgatcccgtt ataacaatgc tgggacaaca
     1681 tgctgaagat gatattatta atagaataag agcagaatta aaattagatg atccctggca
     1741 tgttcaattt attaattatc taaaaagaac tttaagaggt gatttgggga gatcctatat
     1801 ttcaggagtt tcagttactg atgctcttaa acaaaaactt cctaacactc ttaaattggc
     1861 tgttactgca gttatagtct ccactcttat gggagttttt ataggcgtta tttcagctgt
     1921 ttatcaaaat acctggattg atcatgcagc tatgacattt gcacttttat ttatatcgac
     1981 accagtattc tggtttggaa tgatattaat ttatttcttt tcaatgactt taggctggac
     2041 tcctatttca ggaatgggag atggaggttt gagtttacag catttaattc taccggcaat
     2101 tactctaggt agtagatttg cagctttttt agctcgttac actcgctcag ttatgttaga
     2161 agttattact caggattata ttagaacagc ccgatcaaaa ggtttaaagg agagaaaagt
     2221 tatcttaagt catgctttaa gaaatgtgat gatacctgtt gttactgtag tgggtatgaa
     2281 ctttgcttct cttttaaatg gctcagtttt aacagaaacg atttttgcct ggcctggttt
     2341 tggtagatat gtagttactg ccataaaaaa gagagacttc caggttatag caggctgtgt
     2401 tttggttggt tctttgattt ttgtattttt taatctaata gtagatgtaa tatatgtagg
     2461 acttgatcca aggatcaagt atgattgata aggagggaaa aatctaacat gcaagcaaat
     2521 aaaaaagata ataaaactgc tagagataaa aagcctaaaa gtttatggca ggatacctgg
     2581 aaaagattaa aaagaaataa agcggcaatg ataggtcttg taatggttat tttgtttata
     2641 cttattgcta tttttgctcc atttttagct ccctatgatc caaatgaaat agatttaact
     2701 tctgtactaa aagcaccttc tgcagatcat tggctaggga cagattatta tggtagagat
     2761 cttaccagta gaataattta tggttcgcgt atatctttga gtgttggaat tatagtacag
     2821 acaatagccc ttactattgg tactcttatg ggttctcttg ccggttatta tggtggtaaa
     2881 gtagatatgt ttattatgcg gattgttgat atagttatga gttttccaag tttgttatta
     2941 acaatagcaa ttatggttgc cttaggtccg agtttatata atgtgttttt ggctttagga
     3001 gttgtttggt ggaccagaac ggccagaatt attagaagtg aatttatgcg aaatagagaa
     3061 aaagaatatg ttgatgctgc tcgagcttta ggaaataatg attttattat aatgtataaa
     3121 catattttac ctaattgtat ggctcctctt attatttctt ttacaatggg gatagcatct
     3181 tcgattatgg ctgaagcttc acttagtttt cttggtttgg gagctcagga acctacacca
     3241 agttggggct ctattattaa taatggttta caatacttaa gaacaaaacc ctggtattct
     3301 ttatttccag gtttagcaat cgcctataca gttcttggat ttaatttact tggagatgga
     3361 ttacgggatg ctcttgatcc aagaatgaaa aaataatttt aaaatgggaa ggtgaagatt
     3421 ttgagtaaag aaaagttaat agatgtaagt aatctaaaga cccacttttt tacagaagaa
     3481 ggtgtagtaa aggcagtaga tggagtagat tttgagatct atcctggaga gacaataggg
     3541 atagtaggag aatctggatg tggaaagagt gtaacttcac tatcaataat gagattggta
     3601 gaatcacctc caggaagaat agtagatgga agtataatgt ttgaaggaga agatatagta
     3661 aaaaagaaac aa
//
LOCUS       DATGCJ010000188         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_385259, whole genome shotgun sequence.
ACCESSION   DATGCJ010000188 DATGCJ010000000
VERSION     DATGCJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385259"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VKN64_08815"
     CDS             complement(<1..393)
                     /locus_tag="VKN64_08815"
                     /inference="COORDINATES: protein motif:HMM:NF039831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HMA60379.1"
                     /translation="MNKKILYLVIILTLIVSLIGIRGRMMVEQSNNNVEMAFDYKSLL
                     EMRDEYDNQTLALQDLKNAGINSIALTPIDLKRLIKRGQVVHLTISDINKRKILEDNQ
                     SAFWQEFTLGEQSAFLVFEAKNNYYNNLY"
     gene            complement(415..1528)
                     /gene="prfB"
                     /locus_tag="VKN64_08820"
     CDS             complement(join(415..1458,1460..1528))
                     /gene="prfB"
                     /locus_tag="VKN64_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_108076435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMA60380.1"
                     /translation="MNGNYEERIAALKERYQDLRDLLDPAELVSKRDELEERMVAPDF
                     WDDNEEATRISKKVDSIKNKLNLLEQLKDDIEEIEVYKELASEEEDDSLLKQVHQKLD
                     NLDQRLERLELKTRLNGEHDKNNAILSIHPGAGGTESQDWAAMLLRMYDRWAQKEDFE
                     VKTLDFQSGDEAGIKRVTILIEGEYAYGYLKGEAGVHRLVRISPFDSSGRRHTSFASV
                     DIMPEIEEDIEVDIDENNLKIETYRASGAGGQHVNKTDSAVRITHQTTGVVVQCQNER
                     SQHKNKQMAMKILKTRLMELKKEAQAEKIDELRGEHKEIAWGSQIRSYVFHPYNMIKD
                     HRTDVEEGNTEKVMDGYLDEFIEAYLKQNNSGELNI"
     gene            complement(1548..4127)
                     /gene="secA"
                     /locus_tag="VKN64_08825"
     CDS             complement(1548..4127)
                     /gene="secA"
                     /locus_tag="VKN64_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMA60381.1"
                     /translation="MFDFVKNLFKDPNEKELERLQEIVDEINALESDMQKLSDAELKA
                     KTAEFKQRYQDGESLDDLLPEAFAVVREASQRSTKEEFRHFDVQLMGGIALHEGKIAE
                     MKTGEGKTLVATLPVYLNALTEKGVHVVTVNDYLAKRDSEWMGQIYEFLGLDVGVVLS
                     GMTPEERKKAYDADVTYGTNNEFGFDYLRANMATDPDNVIQHDFHYAILDEVDSILID
                     EARTPLIISGPVQETSKDYRKFNRIIPNLKEEQHYELDEKNNSVTLTEEGVEKVENYL
                     NVDNLFDEDNFRLHHQVNQALRAHTLMKKDRDYIVDEGKVKIVDEFTGRVMEGRRYSE
                     GLHQAIEAKEGVSVQKGSQTFARITLQNYFRMYDKLAGMTGTAKTEEEEFIKIYGMEV
                     VVIPTNEPLVRDNKPDLVFKDEESKFRAVVEEIEERHEKGQPLLVGTVDIDKSEKLSR
                     MLNRKGIQHNVLNAKNHKKEAEIIKDAGQKNSVTIATNMAGRGTDIVLGEGVVELGGL
                     HVLGTERHESRRIDNQLRGRAGRQGDPGSSQFFVSLEDDLLRLFGSDNITALMDKVGM
                     EDDQPIQHKMITNAIERAQKKVEGRNFDIRKAILQYDDILNKQRKIIYEQRSDVLFSD
                     NMDEIIFGMLEQLVEDITESYLSEDIHPSDWDRAGLVDYLNKFYFSEELTAEDIEDKN
                     KEEIKEYLIEFGKQNYKDKEEKFKEENMNKIAKYIALRVIDRNWMSHLDNMDELRQGI
                     GLRAYGQKDPLTEYKFESYDMFNEMTSSIREDIIRSIFRIEVHEDGQLDRKQTSRNLN
                     YSDGGTISALATAKNKAQGQRQTTTNKDKKTHSTVVKPDEPGRNDPCPCGSGKKYKKC
                     CGR"
     gene            complement(4400..4963)
                     /gene="raiA"
                     /locus_tag="VKN64_08830"
     CDS             complement(4400..4963)
                     /gene="raiA"
                     /locus_tag="VKN64_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMA60382.1"
                     /translation="MNITVYGKNFDITPSLKDYAREKMEKLEKYFQGEPMEVHITMEV
                     EKERHIVEVTAYVKGLILRGEEETTDMYASLDGVIDKVERQIHKYKTRIHNKMIERKQ
                     EYKEEYMEEREEMVRKKKEPEEDEFEPKIVKTKSFALKPMDVQEAVMQMDLIGHDFFV
                     FSNANTNEVNVVYKRKDGNYGLIEPTF"
     gene            complement(5111..5323)
                     /locus_tag="VKN64_08835"
     CDS             complement(5111..5323)
                     /locus_tag="VKN64_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HMA60383.1"
                     /translation="MIYTGTVKWFDAKKGYGFIEREEGDDVFVHFSAIQDDGFKTLED
                     GEKVEFEIVEGDRGPQAAQVVRVEEE"
     gene            complement(5457..>5740)
                     /locus_tag="VKN64_08840"
     CDS             complement(5457..>5740)
                     /locus_tag="VKN64_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HMA60384.1"
                     /translation="SGRSFSEILPDKKKKLEDQFVYKEIINLVFQEIERELTELEKNV
                     FYEYLDDRTYKEISDKLEINVKTVDNALQRARKKIEEIKENNNLEDLVI"
BASE COUNT         1477 a         1296 c          850 g         2117 t
ORIGIN      
        1 atagaggtta ttatagtaat tatttttggc ttcaaatact aaaaatgcac tttgctcacc
       61 tagggtaaac tcctgccaga aagcagattg attatcttct agaatttttc tcttatttat
      121 atcactgatg gttaaatgaa ctacctgacc ccttttgatt aacctcttta aatcgattgg
      181 ggttagggca atcgaattaa taccagcgtt ttttaaatcc tgtagagcca gggtctgatt
      241 gtcatactca tcccgcatct ctaacaggga cttataatca aaagccattt ccacattatt
      301 attggactgt tcgaccatca tcctgcccct gattccaatt aaggaaacga tcaaggtcag
      361 gataataact aggtataata tctttttatt cataaactgc ctcctgagct caaattaaat
      421 atttaattct ccactattat tttgcttcaa atatgcttct ataaattcat ctagataacc
      481 atccattacc ttttcggtgt taccttcttc cacatcggtt cgatggtctt ttatcatatt
      541 ataggggtgg aaaacataag aacggatctg actgccccag gcaatctctt tatgttcacc
      601 ccttaattca tctatctttt cagcctgggc ttcttttttt aactccatta agcgggtttt
      661 taagatcttc atcgccatct gtttattttt atgctgagat ctctcgtttt gacactgaac
      721 aaccacacca gtcgtctggt gggtaatccg gaccgctgaa tctgtcttat ttacatgttg
      781 gccgccggct ccactggccc gataggtttc tatttttaag ttgttctcat ctatatcaac
      841 ttcaatatct tcctcgattt ccggcattat atctaccgaa gcaaaagaag tatgcctgcg
      901 tccggatgaa tcaaagggag agatcctcac cagccggtgt acaccggctt cacccttcag
      961 atagccgtaa gcatattcac cttcaatcaa aatcgtgacc ctcttaatac cggcttcatc
     1021 accgctttga aagtccaggg tttttacctc aaagtcttct ttttgggccc atctgtcata
     1081 catcctgagc agcatggcag cccagtcctg agactcggtt ccaccggcac cggggtgtat
     1141 agataagatt gcgttgtttt tatcatgttc accgttaagc ctggttttta gttccagtct
     1201 ttccagcctc tggtctaaat tatctaattt ctgatgtacc tgttttaaaa ggctgtcatc
     1261 ctcttcttcg gaggcaagtt ccttatatac ttctatctct tctatatcat cttttaattg
     1321 ttctaataag ttgagcttgt tttttatact atccactttt ttgctgattc gggtggcctc
     1381 ttcgttgtca tcccaaaaat caggagcaac cattctttct tctaattcat ctcttttaga
     1441 gaccagctcg gcagggtcaa agtagatccc tcaaatcttg gtacctttct tttagggctg
     1501 cgattctttc ttcataattt ccgttcatct aaaactcctc cttcatatta tctgccacag
     1561 catttcttat attttttacc actgccacag ggacagggat catttctacc cggttcatca
     1621 ggtttaacca cggttgaatg agtcttctta tctttattgg tagtagtctg tcgctgccct
     1681 tgcgctttat ttttagcagt tgcgagggca cttatcgtac cgccatcact ataatttaag
     1741 ttacggctgg tctgttttct atccaattgc ccatcttcat ggacttctat tctgaagata
     1801 cttctaataa tatcctctct tatcgaactg gtcatttcat taaacatatc ataggactcg
     1861 aacttatact cggttaaagg atctttttgg ccgtatgcac gcaggccaat tccctgtcta
     1921 agttcatcca tattatcaag gtgtgacatc cagtttctat cgataactct cagtgcgata
     1981 tattttgcaa tcttattcat gttttcttct ttgaacttct cttctttatc tttatagttc
     2041 tgcttaccaa attcaattaa atattcttta atctcttctt tgttcttatc ttcaatatct
     2101 tctgccgtta attcttccga gaaatagaac ttgtttagat aatccacaag acctgcccga
     2161 tcccagtcac tggggtgaat atcttcagaa aggtaacttt ctgtaatatc ctcgacaagc
     2221 tgttccagca ttccgaagat gatctcgtcc atattatcgg aaaagagaac atcgcttctc
     2281 tgctcataaa tgatcttacg ctgtttattt aagatatcgt catactgtaa gatggccttt
     2341 ctgatatcga agtttctgcc ttccactttt ttctgggccc tttcgatcgc attggtaatc
     2401 atcttatgct ggataggctg gtcatcttcc ataccgacct tatccattag ggccgtaata
     2461 ttgtcggatc cgaagagcct taagaggtca tcttctaagg agacaaagaa ctgtgaggaa
     2521 cccggatcgc cctgacggcc ggcccgtcct ctcagctggt tgtcgatcct gcggctttca
     2581 tgtctttcag tacctaatac atgtagacca cctaactcga ctactccttc acctaagacg
     2641 atatcggtac ctctaccggc catgttagtt gcaattgtta cactgttttt ctgaccggca
     2701 tccttaatta tttcagcctc ttttttatgg tttttagcat ttaagacatt atgctggatg
     2761 cccttgcggt ttaacatcct gcttaatttt tccgatttat ctatatcgac agtacccact
     2821 aagaggggct gacctttttc gtgtctttcc tctatttctt ctacaactgc cctgaattta
     2881 gactcctcat ccttaaagac aaggtccggc ttgttatctc tgacaagtgg ttcgttggtg
     2941 ggaataacga caacttccat cccatatatc ttgataaatt cttcttcttc agttttggca
     3001 gtaccggtca taccggctaa tttatcatac attctaaagt agttctgtaa ggtaatgcgg
     3061 gcaaaggtct ggctgccttt ttggacagat acaccttctt tggcttcaat tgcctggtgt
     3121 aagccctcac tgtaccttct accttccatt actcggccgg taaattcatc gacaatctta
     3181 actttacctt catctactat atagtctcta tcttttttca tcagggtatg ggctcttaag
     3241 gcctggttaa cctgatggtg taatctaaag ttatcttcat caaagaggtt atcaacattt
     3301 aaataatttt ctaccttttc tacgccttct tctgttaagg taacactatt gtttttctca
     3361 tctaattcat aatgctgttc ttcttttaag ttcggaatta ttcggttaaa cttcctgtaa
     3421 tccttggaag tttcctgaac cggtcctgaa atgatgagcg gtgttcttgc ctcatcgatt
     3481 aaaatactgt ctacctcatc taagatagca tagtggaaat cgtgctggat aacattatcg
     3541 ggatcggttg ccatgttggc ccgtaagtaa tcaaaaccga actcgttatt ggtaccatat
     3601 gttacatcag catcatatgc tttttttctt tcttctgggg tcatcccgct taatactact
     3661 cctacatcta aacctaaaaa ttcataaatc tggcccatcc actcactatc tcttttggct
     3721 agatagtcat taacagtaac tacatggacg cccttctcgg ttaaagcatt taagtataca
     3781 ggtagggtag ctaccagggt cttaccttcc ccggtcttca tttcggcgat tttaccctca
     3841 tgtagtgcga taccacccat caactgtaca tcaaaatgtc taaactcttc cttggttgaa
     3901 cgttgagaag cctctcttac gaccgcaaat gcttccggta atagatcatc cagactttct
     3961 ccatcctgat atctctgttt aaattcagct gtctttgctt tcaattccgc atcggaaagt
     4021 ttctgcatat cgctttctaa cgcatttatt tcatcgacaa tttcctgtaa tctttccagt
     4081 tctttttcat tgggatcttt aaataagttt ttcacaaaat caaacatttt tttaccacct
     4141 gtttttttat tatagatcaa aaaatttaag tctaaatcat tacaaattta cattatatta
     4201 taacattaat tggttaaaat ctcaatctat tcacttatct atcatataac agaaatgtct
     4261 caatattatg ggatttatta tttcttttcc ccagtataat tctaatgctt tcatatttta
     4321 aataagaatt aattatatgt taaaaggccg cccgttttaa agggcggcct atttacaatc
     4381 tatttattta tcttaatatt taaaaagtgg gttcaatcaa cccataattt ccatcctttc
     4441 ttttatatac tacattcact tcatttgtat ttgcatttga aaatacgaag aaatcatgcc
     4501 caattaagtc catctgcatg actgcttcct ggacatccat cggttttaaa gcaaaactct
     4561 ttgttttaac gatctttggt tcaaattcgt cttcctctgg ttcttttttc ttacgaacca
     4621 tttcctctct ttcttccata tattcttctt tatattcctg ttttctttca atcattttat
     4681 tgtggattct tgttttgtac ttgtgaatct gtctttctac cttgtcaatg accccatcaa
     4741 gcgaagcata catatcagta gtttcttctt caccgcggag aattaatcct ttgacataag
     4801 ctgtaacttc tactatatgc ctttcttttt cgacctccat tgtaatgtgg acttccattg
     4861 gttcgccttg aaaatacttt tccagttttt ccatcttttc tctggcgtaa tcctttaatg
     4921 aaggtgtaat gtcgaaattt tttccataaa ccgtaatatt cattatctcc actcccttct
     4981 gaactactaa aataaatatt gttgttatta taataattca atattttgaa gtaaaaccct
     5041 tcttttataa aaaaaatcct gattacatct ctgtaatcag gattgtgtgc acatatttag
     5101 gcatctataa ttactcttct tcaaccctca caacttgtgc agcttgaggt cctctgtcac
     5161 cctcaacaat ctcgaactct actttttctc cgtcttcaag agttttaaat ccatcatctt
     5221 gaattgctga aaaatgaaca aaaacatcgt cgccttcttc tctttcaata aaaccataac
     5281 cctttttcgc gtcaaaccac tttactgttc cagtatagat cataaattat attcctcctg
     5341 taaattgttt ctaggaaact taaattccca tttatcttaa tataacacag tataatattg
     5401 ttgtcaaaca agttataaca aaagattaca acttattaca cacccaatat atagtattat
     5461 attacaagat cttctaaatt attgttctct tttatttcct ctattttttt gcgggctctc
     5521 tgcagagcat tatcgacagt cttcacatta atttcaagtt tatctgatat ttctttataa
     5581 gtacggtcat ctaagtattc ataaaaaaca tttttttcta gctcagttaa ttctctttca
     5641 atttcctgaa atactaaatt tataatttct ttataaacaa attgatcttc caattttttc
     5701 ttcttatcgg gtagaatttc actaaaactt ctccccgact
//
LOCUS       DATGCJ010000189         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_118293, whole genome shotgun sequence.
ACCESSION   DATGCJ010000189 DATGCJ010000000
VERSION     DATGCJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118293"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VKN64_08845"
     CDS             <1..660
                     /locus_tag="VKN64_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMA60385.1"
                     /translation="FDKKAPMGITPLNREQKFAYELLLNDDIRIVTMSGKAGTGKTLL
                     ALAAGLQKVINDKYYDRVLIARPVIPMGKDIGFLPGSVEEKLEPWMQPILDNLNLIIH
                     SNKGSYLSIDQLIENNFIQIEALTYIRGRSIPNQYIIIDEAQNLSKHEVKTILTRAGK
                     DSKIVLTGDPFQIDNPYLNKVNNGLSQVANHFYKEQITGHIMLQKGERSEIAKIASEL
                     L"
     regulatory      773..965
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1103..2668
                     /locus_tag="VKN64_08850"
     CDS             1103..2668
                     /locus_tag="VKN64_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HMA60386.1"
                     /translation="MENYGIISLIPPLLAIILAWWSKEVIISLFIGVFSGALILSSYN
                     PIAAFASTLDKYMLGSLADSWNAGIIIFLLSMGGMIGIINKAGGVVAIGEYVAKKAKS
                     IKNTLMATWAMGVLIFFDDYANTLIVGNTMRPITDKMNVSREKLAFVVDLTAAAVSSI
                     VPISTWIAFEVGVIKDGFDKLGIEASAYTTFVKTIPYRYYSIFALLFALIVIYLMKDF
                     GPMYTAEHRARTTGQSLRPGSTPMVSKELEEIEKPEGPSFSIMDAFSPIFVVIIVTLF
                     GLWYNGGGASPDVSIQTAFGDADASVVLLWASFAGGFVAGINALIKGILDISDVVESW
                     IDGAKSMFIATMILILAWSIGSITDDLGTANFPVGILEGNLPAFLIPVLLFIISAFIA
                     FTIGSSWGTVAIVMPLAIPLAHNIGSPMLPAIGAVLTAAVMGDHCSPISDTTIMSSTA
                     SAVDHIDHVSTQLPYALTVAAVAAIFGFLPAGLGISPLISLPVGIIVLYIIVNVFGKS
                     VKEAKLEEVEMEH"
BASE COUNT          878 a          453 c          576 g          953 t
ORIGIN      
        1 tttgataaaa aagcaccgat gggcatcact cccttaaata gggaacaaaa atttgcctat
       61 gagctattat taaatgatga tattcggatt gttacgatgt cgggtaaagc cggaacaggc
      121 aaaaccttac ttgctttagc ggccggatta cagaaggtaa ttaatgataa atattatgat
      181 agagttttga ttgcccggcc tgtgattccg atgggtaaag atatcggctt tttaccgggt
      241 tcagttgagg aaaaattgga accctggatg cagccgatct tagataattt aaatttaatt
      301 attcatagta ataagggttc atatctatca atagatcaat tgatagaaaa taactttatc
      361 caaattgagg ctttaaccta tattagagga agatctatac cgaatcagta tattatcatt
      421 gatgaagctc aaaacctctc caaacatgag gttaaaacca ttttaacaag ggctggtaag
      481 gactctaaga tagtattaac aggtgacccc ttccagattg ataaccctta tttgaataag
      541 gtcaataatg gtctttctca agtagctaat catttctata aagaacagat aaccggccat
      601 attatgctgc aaaaaggtga aagatcagaa atcgcaaaaa tcgccagtga actcctttaa
      661 tataagcagt aagctgcaaa tttgactaat tttgtcatat tacttgacag caaaaataat
      721 atcatataca attatagaaa attaaatagt tgaaattacc tcatctttga ccggatgagg
      781 tagaggcgca gaacttaaga ttagcttttt actcataaag aacctattgg taaaaagtga
      841 aagggaattc tgccgaagct tttaattttt ctggttcgaa aaattaaagg ttgggtctgt
      901 atataagata tgcaggactg tcattgaaat atgaacccgt atttcagtgt tgagctatct
      961 catcaaaagg gaaagaggag gtggtttcta ctggtgcaat ttgaattcat acccagcgga
     1021 aatcccgctg ggttttgtta ttttataaaa aaaatcagca tataaaaact aagaaataaa
     1081 ataaaatgga ggtataatca atatggaaaa ctatggtatc atatctctta ttccaccact
     1141 tttagctatt attttggcct ggtggagtaa agaggtaatt atttcattat ttataggagt
     1201 gttttcaggt gctttaatat tgtcaagtta taatccaatt gctgcttttg catcaacttt
     1261 ggataaatat atgttaggct cactggctga ttcctggaat gccggtatta ttatattctt
     1321 actgtcaatg ggtggtatga taggaatcat aaataaagct gggggagttg tagcaattgg
     1381 tgaatatgta gcaaaaaaag ctaagtcaat taaaaataca ttaatggcta cctgggcaat
     1441 gggtgtatta atcttttttg atgattatgc taatacttta attgttggta atacaatgcg
     1501 gcccataact gataaaatga atgtttcaag agagaaactt gcctttgttg ttgatctcac
     1561 agcggcagct gtttccagta tcgtacctat ttctacctgg attgcctttg aggtgggggt
     1621 tattaaagac ggctttgata aattaggtat tgaagccagt gcttatacta cttttgtaaa
     1681 gaccatacct tatagatatt attcaatttt tgctttactt tttgcactta tcgtaattta
     1741 cttaatgaaa gactttggcc cgatgtatac agccgaacat agggcaagga ctaccggaca
     1801 gtccctaaga ccgggttcaa cacctatggt ttctaaggaa ctagaagaga ttgaaaaacc
     1861 agagggccct tcttttagta ttatggacgc tttttctcct atttttgttg ttattatagt
     1921 aaccttattc ggtctttggt ataacggggg aggggcttct cccgacgtta gtattcaaac
     1981 tgcctttggt gatgcggatg ccagtgtagt attattgtgg gcctcctttg caggtggttt
     2041 tgtagctggt ataaatgcat tgattaaagg cattttagat atatcagatg tagtggaaag
     2101 ctggattgat ggggcaaaat cgatgtttat tgcgacaatg atcttgattt tagcctggtc
     2161 aatcggaagt atcactgatg acttgggtac agctaatttt ccagttggta ttctagaagg
     2221 taatttacct gcctttttaa tcccggtcct tctatttata atttccgctt ttatagcatt
     2281 tacaataggg agttcctggg gaacggttgc catcgtgatg cctttagcta ttcctttagc
     2341 tcataacatt ggctcaccaa tgctgccggc aattggggca gtcttaacag cagcggttat
     2401 gggagatcat tgttctccaa tttctgatac aaccattatg tcctctacag cttctgcggt
     2461 agaccatatc gaccatgttt ctacccagct gccctatgcc ttaacagttg cagctgtggc
     2521 ggccatattc ggctttttac cggccggttt aggtatatcc cctctaatta gtttaccggt
     2581 tggtattata gtactttata taatagtcaa tgtgtttggt aaaagtgtta aagaagcaaa
     2641 attggaagaa gtagaaatgg aacattagat aaaataataa attaacttac ccataattat
     2701 ggtgcggttt ttataccgca ctttttcttt ttttagcagg gatagaagct ttcttatcga
     2761 aattattata tagcagttaa ataaggtggt ttaataatga aaaaattttt aacagtagta
     2821 attgtattaa taatatgttt ttttatagga ttttctattc
//
LOCUS       DATGCJ010000190         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_71428, whole genome shotgun sequence.
ACCESSION   DATGCJ010000190 DATGCJ010000000
VERSION     DATGCJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71428"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1730
                     /locus_tag="VKN64_08855"
     CDS             117..1730
                     /locus_tag="VKN64_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60387.1"
                     /translation="MEVAKKEKSSHIGTCEQRDNYSTLNNSISLNMRFCKVLFDNLEG
                     YVEIAELKNGKMPKQYYNSVEELKEYDPPQDKDVFLGVFSRKYPSGKIEAVNQTNAIW
                     LDFDNISDPAALEYILDNKALPEPSIVVNSGHGLHAYWLLDKPAGREVRPVVKKLINI
                     TGADPKGVNENRLMRIPGTINNKKEPVKCEVIQMTDRSFKLDHIADLLGVKAELKDKS
                     KNVAEALNIDYEVIRNNVKSPCIKNIMGGVPEGERNWLLGRLTKYLKHKLDLSKKDAR
                     KVIRVWNTKNNPPQPEGELLASFNEYWHNKKYNLLGCFIKDKEGNPIPQLQQILEKYC
                     NRDNCNSDGQIEFVDGESIVEFNNRLINKIKNISVNSFIIYSILSQNEQGLSAKKAAN
                     IMGVSQKTFKRNIGELIKLGYAKKKKGIPQRKIPDLYYFTKQGTFGLSNTVIRYATMR
                     AMLAELRNGDIRPLDIKIYILLRYYRKRSLINKAYPSTTTLAEKLGTTRAEISKRVNH
                     LERRDYFRIERGKYRSNLYKFKFDSNKKN"
     gene            2192..2419
                     /locus_tag="VKN64_08860"
     CDS             2192..2419
                     /locus_tag="VKN64_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60388.1"
                     /translation="MKDKALYSYEKRLDSLKSARQFQAKAIVKYDQGEISAEKLNTIS
                     KAMNTLLRALEKSNLELRVDELESILKRKSA"
     gene            2432..3079
                     /locus_tag="VKN64_08865"
     CDS             2432..3079
                     /locus_tag="VKN64_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60389.1"
                     /translation="MRRELQNIASEIDNFVNEVQQIKSVPDGDIGVNIKNKQIKEKEK
                     QFKNYADKMFESAENKLNKQAEKAQNKLKKMQGDNYQKRQYYYTKANQELSNYKSPID
                     YLKDKKEISNNQIELQEARKVALSKAKATDDGDYQRLKEIVVDTMGSDELEARKELAK
                     VKIKRKNLKGAVSSINFDLDQIKNGNVSSAKTSLIAFSETDNLEQKATKKISETL"
BASE COUNT         1366 a          378 c          619 g          830 t
ORIGIN      
        1 ccaaatatta gtaactcttg atgaaatggg atataatact aaaaagatta gtgatcaata
       61 ccaagaattt aggaaagaga aggcagaaaa gctaatatct taatgaggtg ataagattgg
      121 aagttgctaa aaaagaaaaa agctcacata taggcacatg tgagcaacga gataactatt
      181 caactcttaa taatagtata tcattgaata tgagattttg taaagtatta ttcgataatt
      241 tagaaggata tgtcgaaatt gcagaactca aaaatggtaa gatgcccaaa caatattata
      301 attcagttga ggagttaaaa gaatacgatc cgccgcagga taaagatgta tttttaggag
      361 tattcagcag aaaatatcca agcgggaaaa ttgaagcagt taaccagacc aacgcaatat
      421 ggttagactt tgataatata agcgatcccg cagcgttgga gtatatacta gacaataagg
      481 cactcccaga gcccagtata gttgttaata gcggacatgg tttacatgca tattggttat
      541 tagataagcc tgctggacgt gaagtcagac cagttgtaaa gaaattaata aatattactg
      601 gtgcagatcc gaagggagta aatgaaaata gactcatgag gataccagga acaattaaca
      661 ataaaaaaga gcctgttaag tgtgaagtaa ttcagatgac cgacaggagc ttcaaattag
      721 accatatagc agacttatta ggagtgaagg ctgaactaaa ggataagtct aaaaacgttg
      781 cagaagccct taatatagac tatgaagtaa taagaaacaa cgttaaaagc ccatgtataa
      841 aaaatattat gggtggtgtg cctgaaggtg aaagaaattg gcttttaggt agattaacaa
      901 aatatttaaa acacaaatta gatttatcca aaaaagatgc ccgaaaagtt ataagggttt
      961 ggaatacaaa aaacaatcca ccacagcccg aaggtgaact attagcaagt tttaacgaat
     1021 actggcataa caaaaaatat aatttattag ggtgttttat taaagataaa gagggcaacc
     1081 ctataccgca gctacaacaa atattagaaa aatattgtaa tagagataat tgcaactctg
     1141 atggacaaat tgaatttgtt gatggagagt caattgttga atttaataat agattaataa
     1201 acaagataaa aaatatttct gtaaacagtt ttataattta tagtattcta tcacaaaatg
     1261 aacaagggct ttcagcaaag aaagctgcaa atataatggg tgtttcacaa aaaacattta
     1321 aaagaaatat tggagaatta ataaaattag gttacgcaaa aaagaaaaaa ggaattccgc
     1381 aacgcaaaat accagattta tattatttta caaagcaagg aacgttcgga ttaagtaata
     1441 cagtaataag atatgctacc atgagggcaa tgcttgcaga gctccgcaac ggagacataa
     1501 ggcctttaga tattaaaata tatatattat tgagatatta caggaaaaga agtttaataa
     1561 ataaagcata tccatctaca acaacacttg cagaaaaatt aggtactacg agagcggaaa
     1621 ttagtaaaag agttaatcat ttagagagaa gggattattt caggatagaa cgcggtaaat
     1681 atagatctaa tttatataag tttaaatttg attctaataa aaaaaattag agagtaaaga
     1741 atactatttt gacggttagc atataattta tggtggacac gtagcacaca tgtaattaga
     1801 gagtaaagaa taccgtttta acagtaaggg tagcttctat ggtggacacg tagcacacat
     1861 aagtacttta gaagggttaa aactgtctat tgagttaaaa gagaatatag agagagtaaa
     1921 ggataatgat cctggatagc tcccaaaaaa gttggaagtc gcagcttgag ggggctattt
     1981 tgtatgtaaa gatatataaa aaggtggtgt attatgcaag atttactatt atcagcaata
     2041 ttaataagtg gtttatgtgt agtcagcttt aaagtaggtg gaattatatc aacaaaaata
     2101 aataaagtgt agtgatatta actagttgaa aaatgtagtc taatgaacgg tgtaattact
     2161 acaatgacag taaagaaggt gtaaaaaaat tatgaaggat aaagctctat attcatatga
     2221 gaaaagatta gatagtttaa agtcagcccg tcaatttcaa gcaaaagcta ttgtaaagta
     2281 tgatcaaggg gaaatatcag cagagaagtt aaacactata agtaaggcta tgaatacact
     2341 attaagggct cttgagaagt ctaatttaga attaagagtt gatgagttgg aaagtatttt
     2401 aaaaaggaaa tcagcataag gagtggataa aatgagaaga gaattacaaa atatagcaag
     2461 tgagattgat aactttgtga atgaagttca acagataaaa agcgtaccag atggagatat
     2521 tggagttaat ataaaaaaca aacaaattaa ggaaaaagaa aaacaattta aaaattatgc
     2581 agataaaatg tttgagagtg ccgaaaataa gctcaacaaa caagctgaaa aagcacagaa
     2641 caaacttaaa aagatgcaag gtgataacta tcaaaaaaga caatattact atactaaagc
     2701 aaatcaggag ctatctaatt ataaaagccc tattgattat cttaaagaca aaaaagaaat
     2761 atctaataat cagatagaat tacaagaagc aaggaaagtt gcattatcta aagcaaaagc
     2821 tactgatgat ggagattatc aaagattaaa agaaattgta gtcgatacta tggggtctga
     2881 tgaactagaa gcaagaaaag aactggcaaa agttaaaatt aaaagaaaaa atttaaaagg
     2941 tgcagtaagt tcaataaact ttgatcttga tcagattaaa aatggtaatg taagttcagc
     3001 gaaaacttca ttaattgcat tttctgaaac cgataattta gaacaaaaag cgaccaaaaa
     3061 aataagtgaa acattatagg aggttagcaa atggaaactt ataacggtga actaaaacaa
     3121 gataagagta aaactcacaa aggtaaagtg cctgaaggtt ataagagaaa ccaattcggg
     3181 aaaatagtcc caa
//
LOCUS       DATGCJ010000191         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_27495, whole genome shotgun sequence.
ACCESSION   DATGCJ010000191 DATGCJ010000000
VERSION     DATGCJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27495"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1159)
                     /locus_tag="VKN64_08870"
     CDS             complement(200..1159)
                     /locus_tag="VKN64_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMA60390.1"
                     /translation="MKFTRFILVLFVFGLLLVGTSIDIMAEDDLKIVPIYEGSELIYD
                     DIFGFEELPVILGEDTVNILEGYIRRFWLKAPEGRSPYEVIRNYEAAIDNIDGRVLFK
                     TRDPQSIEIDEIDFSEYYNTTRQDRGLSTNVFSFTKYPEEITEYMVSRVTVDQVEKYI
                     IVAAGRGHWAAGQADETYFEIVILELEGMEMDMVTMEYIKQGIEVDGRVAIYDIYFDT
                     GESRVKADSEEALSVIAEFLLKNSGKRYLVVGHTDFVGSYEMNLNLSESRAEAVVTKL
                     LNEYGISQKQLISTGVGPAAPVMSNQTEEGRAMNRRVEIVELE"
     gene            complement(1783..2121)
                     /locus_tag="VKN64_08875"
     CDS             complement(1783..2121)
                     /locus_tag="VKN64_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60391.1"
                     /translation="MRLKWNPLFDDTSRLFSPVIDAGTSSELKLSFKHFNVYVFGSYE
                     LLVQTSTDDGTTWNTEWNVMVTGDIGPETIYIELDHLAGETFRIAWVFDGYTLYNDDW
                     YIDDIIVEEN"
     gene            complement(2249..2758)
                     /locus_tag="VKN64_08880"
     CDS             complement(2249..2758)
                     /locus_tag="VKN64_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMA60392.1"
                     /translation="MNNPQDYLFVGVDTHKAQHTAVVINCFHQKIGFAQTPNNPADFD
                     SFLTRLKTLTHNDEKLVFGLEDTQGLGYSLAQWLIKKDYTVKEVNPVQTKRERKQSTS
                     PDKSDKADAEAVANILLAEYDKLPVIKDDVTFKAIRQLNNQRKNLVKEQTRVKNLPKS
                     LKDRWEWYG"
     gene            4296..4583
                     /locus_tag="VKN64_08885"
     CDS             4296..4583
                     /locus_tag="VKN64_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60393.1"
                     /translation="MSLLILDFNGNGITELGMNDIAVQDATATNGDWIRLADAFGTSG
                     WDNITLEDQGSGNYEVLWDSSGVYGDYDELWNVRVCGVVITENLDMAITGS"
     gene            5196..>5375
                     /locus_tag="VKN64_08890"
     CDS             5196..>5375
                     /locus_tag="VKN64_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60394.1"
                     /translation="MVASSSKNMSRIELVYTVNANMIKKLKQLDKEAIPESCLSYLEK
                     GHKKETVYKTRDKDIE"
BASE COUNT         1775 a          941 c          857 g         1802 t
ORIGIN      
        1 tgtcagtcct atttaacaca aaaacaaggt cccagggata tctataccct aaaaatacat
       61 tatttaagta gaatattgta taaattcaaa ttaaaagttt aatttagaaa tcgaaaatgt
      121 aaatatcgtt attttagctt aaattagtgt gtaaatttat tttttgtggc tactttccga
      181 attcaggtta aacacaatat tattcaagtt caacaatctc tactcgacga ttcattgctc
      241 ttccttcttc agtctgatta ctcattaccg gtgcagctgg accaacacca gttgaaatta
      301 actgtttttg acttataccg tactcattaa gcagttttgt aactactgcc tctgctctag
      361 actcagataa atttaaattc atttcataac tcccaacaaa atctgtatgg ccaactacta
      421 gatatctttt accactattt tttagtaaaa attctgctat tacagacagt gcttcctcag
      481 aatcagcttt caccctggat tcacctgtat caaaataaat atcatagata gctactcggc
      541 catcaacctc aataccctgt tttatatact ccattgtaac catatccatc tccatacctt
      601 ctaattccag gataactatc tcaaagtaag tttcatcagc ctgtcctgcc gcccaatgac
      661 ctctaccagc tgcaacaata atatattttt ctacctgatc aactgttacc ctggaaacca
      721 tatattcagt tatctcttca gggtatttag taaaactgaa aacattagtt gacagccctc
      781 gatcctgtct ggtagtatta taatattcag aaaagtcgat ttcatcaatc tcaatcgact
      841 gcggatctcg ggtcttaaat aatacccggc catcaatatt atcaatcgct gcctcataat
      901 ttctaataac ctcataggga gaacgtcctt ctggagcctt taaccaaaat cttcgaatat
      961 atccttcaag tatattaaca gtatcttcac ctaaaataac tgggagttct tcgaagccaa
     1021 aaatatcatc atagatcagc tcactacctt cataaattgg aacaattttc agatcatcct
     1081 cagccattat atctattgaa gtccctacca acaacagtcc aaaaacgaat aacactaata
     1141 taaacctggt aaatttcatt atatcccccc aatatgatat gttaattaat tttaaaactt
     1201 acacttaaat acaagctaca ccagtttggt aaatcattta ttatttttta agtatattat
     1261 tgatattatc catataaaag gaatttcctc tatttataaa cattttaaag agaaaccttt
     1321 aaaatcttag gctaagtgct cagctttaat ttctttactc tctattctgt attgatacca
     1381 ctttgtgtaa ttataggtgt tatataacgc ctatttttgt gtactcctcg ttcaccttct
     1441 atctgtgggt tttcggtact atattgccat atatcttatt taaaatattt ctggaggggt
     1501 agttttataa aacaaaaaga tacagataga ggaagttgat cctaatctgg cttctctatg
     1561 gcagtttatg acttctataa gtactaccgc ctttgaaagc atctgataag gtacccaccc
     1621 ttataaaaag ctatttttga gcaaaaagga gaacctgcca gcaaatctag ctctctggca
     1681 ggttctctta ttgtttctat tcagctatta cacaatccat cctgttatcc aagttggttt
     1741 atttggttaa caatgtcgat aacatttgaa gatcaccgtt ttctagtttt cttcaacaat
     1801 aatatcatcg atataccaat catcgttgta taaagtataa ccatcaaaga cccaggcaat
     1861 tcggaatgtt tctcctgcaa ggtggtctaa ctctatatat attgtctcag gtcctatatc
     1921 tccagttacc attacattcc attcagtatt ccaggtagtt ccgtcatctg tcgaagtttg
     1981 tactaatagt tcataactcc caaaaacata aacattaaaa tgtttaaagc taagtttaag
     2041 ctcgctagat gtaccagcat caattacagg tgaaaacaat cttgaggtat catcaaacag
     2101 aggattccat ttcaatctca tttcaggagc ctctccacct gcattattag aatttcttac
     2161 gcccaattgg ttctgtctct tgaccatcca acagggattt catttatttc tactccagtg
     2221 aagtcatctt caaaacaata tttcaaattc atccatacca ttcccaccta tcctttaagg
     2281 attttggtag gtttttaacc ctggtttgtt ctttgactaa attctttctt tggttgttaa
     2341 gctgtcttat agccttaaaa gtgacgtcgt ctttgatgac aggtagttta tcatattcag
     2401 ccaaaagaat gtttgcaaca gcttcagcat cagctttgtc ggacttatcg ggtgaagtgc
     2461 tttgttttct ttctctttta gtttgaacag gattaacttc tttaacagta tagtcttttt
     2521 taattaacca ttgtgcgagt gaatagccta gtccctgggt atcttcaagt ccaaagacaa
     2581 gtttttcatc attatgggta agggttttaa gcctggttag aaacgagtca aaatcagctg
     2641 gattattggg agtctgagcg aatccgattt tttgatggaa acagttaatt acaacagcag
     2701 tatgttgagc tttatgagtg tcaacaccaa caaaaagata atcttgagga ttattcatgt
     2761 tgttcagctt ctttcaagtt ttataagtca tcatcaggcg tgccctattg taatagcgtt
     2821 aaaaccgcag cgttcaattg acgtctctga tgatgtaaat caggcaggtg gcagtctaaa
     2881 taaaatcgtt actctaaacg tacagagccg caaggagatt tatagcctga cctgtctgga
     2941 ataacctttt tcaaaaaaca aaacgagatg gaatgattaa caaattttcg acttaggggg
     3001 gatcctcccc cccaagaata aaacaatagg aggtatttaa acattttaaa attttaacac
     3061 taaaagatcc ccaatctttt attttatccc tattttaatg ccgatggatc accgaatgcg
     3121 gtaaatgtct gccgaaatcc ttaaagaatg gatggcaatg atatttttac taatttagaa
     3181 gaataataat atctctatca aggagagcat aggctatctt caaccggtag ttttggcata
     3241 tataatgcaa gtggtgataa aattaagata gccttaatat atgaactggg ggcaggagca
     3301 gaatatataa ctggaatagc ttattatgac catcctgata atagtattgc taactccaca
     3361 aatacaacca cccgtgtatg gatttgacga taaaagtaaa ccatatgttg atctgtggtc
     3421 cagcaatttg tgtcaatcta agggtgaagg ttggataaag gtcattatcc cttctcaaga
     3481 acatattgaa ataggaaact ataattataa ttgaattatt gttatattta atatcagttc
     3541 aaataattgg actggactcg gaacagataa cgtcacttct aatccagatg cagatatata
     3601 tgactaaggt cctggttgga cacatataga tagttatatt ggcaattgat aattagaatg
     3661 tttggtcagt tatacagatt aacttttaaa aattatagct taacttaaaa attcgtcagt
     3721 aaatattatc tgctatagag tttttttatt tataattcta tttataataa ggctaacagg
     3781 atttgaacct gtatcctaca gattagaagt ccccaaaata ttttttaata acttttacag
     3841 aactgctaat attgctatta tttcagcaac tttagacctt tgtaaaatat ttattttgtg
     3901 gcaatttaaa ttacttttac aagctttcgg cacttatcgg cacttgaatt aaactatttt
     3961 tctaccttaa ttatatcaac ataattaaaa atctatgtca atgattttgt tataatttca
     4021 agaaggtatt tttcagtaca ccttgaatta ataattatac aatataagta taattacaat
     4081 actagaaagg aggtatttaa accttttaac actaaaagat ccccaatctt ttagtttatc
     4141 cctattttaa tgctgatgga tcgccgtatg cggtaaaagc cgcccgtacg gtgaagacct
     4201 accaaaatcc ttaaaggata ggtgggaatg gaattggagt agatgctgaa agcagttatg
     4261 ccttatggga agataacgaa gatgggacag gaacaatgtc tttattaata ttggacttta
     4321 atggaaatgg tattacagag cttggtatga atgatattgc tgtacaggac gctactgcta
     4381 ctaacggtga ttggattaga ttagctgatg cttttggtac ttcaggatgg gataatatta
     4441 ctctagagga ccaaggtagt ggcaactatg aggttttgtg ggattctagt ggtgtgtatg
     4501 gcgactatga tgaattatgg aatgttagag tttgcggtgt agtaataaca gaaaatctag
     4561 atatggcgat aacaggcagt tagtagaata gaatttaaaa taaacactag tatttacttc
     4621 aaaaagagaa gatgacaaat aaataagcaa aaactaagca gattaggcta gtaactcagc
     4681 ttaatctgct ttttttaatt aaattataga gaattgtact acaaaaatat agaaatattt
     4741 accaacagat atcttttgat gatactgtaa ccaatatgct ggtttatcta aaagatttcc
     4801 ttaaagaaag ctgggctcat acttttcaaa aaataatctt tccagctatt aatgaagata
     4861 gatttgaagt tttatattgt gataaaccat caagacctaa ttcttcggta aatgtaatta
     4921 ttagggcatt gatcttaaaa gaaatatatt aactttcaga tgttgtaatt ttaggttcta
     4981 tttactttga tgacagattc cagtatgcat taagtttaac cagctttgat aaaacgccag
     5041 tttctattaa tccctttact aattttagag ctatatttta tgtctatgaa gaagaaattg
     5101 gtgtcgattt gattaaggaa gaagtagaaa gtctctcgta acttatcgca gaaatactta
     5161 aaataggcgc tgaaaaaatc caggtggact cttttatggt tgcttcttct agtaaaaaca
     5221 tgtccagaat agaactagtc tatacagtca atgctaatat gattaaaaag ttaaagcaac
     5281 tagataaaga ggcaatacct gaaagctgcc ttagttatct cgaaaaaggc cataaaaaag
     5341 aaaccgtcta taaaacacga gacaaagata ttgaa
//
LOCUS       DATGCJ010000192         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_71846, whole genome shotgun sequence.
ACCESSION   DATGCJ010000192 DATGCJ010000000
VERSION     DATGCJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71846"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..826)
                     /locus_tag="VKN64_08895"
     CDS             complement(38..826)
                     /locus_tag="VKN64_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMA60395.1"
                     /translation="MNIVVCVKQVPDTNEVKIDEEKGTLIREGVPSIINPEDKTAIEK
                     AVKLKEEFGGQVTVISMGPPQAKDALIEAYAIGADQAILLSDKKFAGSDTWATAYTLS
                     QAIKEVGDFDLIFCGRQAIDGDTAQVGPQVAENLDIPQITYVRELNIEGEKVTAKRKL
                     EEGYSIIETELPVLLTVLGDLNKPRYPSIKRIVDAFNNENTVKVWNADDFAVDDDKLG
                     LDASPTQVYDTFVPTRDHEAEILEGTVEEKVGKLIDKLKEEKVV"
     gene            complement(873..2012)
                     /locus_tag="VKN64_08900"
     CDS             complement(873..2012)
                     /locus_tag="VKN64_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMA60396.1"
                     /translation="MDFNLSEEKEMCKKVYKEFAENEIKPIAAEIDENCEFPADNIKK
                     MAEADMMGIPFPQKYGGAGSDTLNYILAVEELSYACATHGVILSAHTSLGAHPIYKYG
                     TEAQKEKYLKPLAKGEKLGAFALTEAGAGTDAAGVKTTAVREGDEYILNGTKIFITNA
                     GAADIFIIHAMTDKSKGTRGISSFIVEADTAGFSLGKKEEKMGINASDTRELVFRDCR
                     IPADNLLGKEGMGFKIAMSTLDGGRIGIAAQALGIAQRALDETVQYVQEREQFGRPIA
                     KFQGLQWEIAEMATKVEAARNLVYKAAKTKDEGRRFSKEAAMAKYFASEAASEVANKA
                     VQLHGGYGYIKDYPVERLMRDAKITEIYEGTTEVQKMVIAGSLLN"
     gene            complement(2062..2490)
                     /locus_tag="VKN64_08905"
     CDS             complement(2062..2490)
                     /locus_tag="VKN64_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HMA60397.1"
                     /translation="MDRGKTVNELEIGEKASFTKTITETDVYNYAGITGDFNPAHVNE
                     SFAKETMFKGRIAHGMIAAGLISTVLGTQLPGPGSVYVAQDLKFTAPVRFGDTVTATA
                     EVEDINEEKNRVTLNTVCTNQKEEVVLTGQAELMPTVKGE"
     gene            complement(2673..3902)
                     /locus_tag="VKN64_08910"
     CDS             complement(2673..3902)
                     /locus_tag="VKN64_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMA60398.1"
                     /translation="MEESILARKPVLNLNQDVIAYEIIFKNSYDNKITDDNIDITAEA
                     INNDVEFIEQTNLSNGKKIFMNFTRDLLKNEVVNFLAKDNLGIEISEDIDYDANLISN
                     IVKLKDEGALIILNDLNPYKPNTTLIKYADILKLDFNKFKEKEHTLIINLLKNKYNKN
                     LKFMAENINEHEQFDQAKECGYDYFQGIFFTKPEVMANRKIPGYKLNYLNFLKELNKP
                     EIDFDKLEDIIKNDMSMTVSLLSTINSAYYGYNVSSIKQASTLLGINGLKKWSLIYLA
                     EGLRNDKPDILFVNTLTRAKFVESAAEDFGVKEKSSELFTMGMLSMMDAFLNKPLEEI
                     IDEISLKGDIKEALLRGEGIYGEILQLAIAFERVKWQEISIIEKTYGLNPKKLYSKYL
                     AAVDFAYETMNMLIENA"
     regulatory      complement(4004..4081)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1158 a          889 c          606 g         1472 t
ORIGIN      
        1 tgatttttaa cattgaaaaa agcctccttc aagattacta aacaaccttt tcttctttta
       61 atttgtctat taattttcct actttttctt caacagtacc ttctaagatt tctgcctcat
      121 ggtctctagt gggaacaaag gtatcataaa cctgagtcgg ggaagcatct aaacctaatt
      181 tatcgtcatc gactgcaaaa tcatcagcat tccaaacctt taccgtgttt tcattattaa
      241 aggcatcaac aatcctcttt atagaagggt atcttggttt atttaaatct cctaatactg
      301 taagtaggac cggtaattcg gtctctatta tactatagcc ctcttctaat tttcttttag
      361 cagttacttt ttccccctct atattaagtt cccgtacata tgtgatctgg ggaatatcta
      421 aattttcagc aacctgaggt cctacctggg ctgtatcacc atcaatggcc tgacgtccgc
      481 agaaaatgag atcaaaatct cctacttctt taattgcctg agataaggta taggcggtag
      541 cccaggtgtc cgaacctgca aactttttat cggaaagcaa gattgcttga tctgccccta
      601 tagcataagc ttcaatcaaa gcatctttag cctggggagg acccatactg attaccgtga
      661 cctgaccccc aaactcttct tttaatttaa ctgctttttc aattgctgtt ttatcttccg
      721 ggttaataat gctgggcacc ccttctctga tcagggtacc tttttcctca tctatcttaa
      781 cttcattggt atctggtacc tgttttacac aaacaacaat attcatcctt atgcctcctt
      841 tttaatatat aattatcttt ttctatttaa aactagttaa gtaaagaacc agctattacc
      901 atcttttgga cttcagtcgt accttcataa atttcagtta tcttggcatc tctcatcagt
      961 ctttcaaccg gataatcttt aatatatcca taaccaccat gcagttgaac cgccttattg
     1021 gctacctcag aagcggcttc agaagcgaag tatttggcca tggccgcttc tttagaaaat
     1081 ctcctgccct catctttggt tttagccgct ttatatacca agttacgggc tgcttcaact
     1141 ttagtagcca tctcagctat ctcccattga agcccctgaa atttagcaat aggacgtcca
     1201 aactgttctc tttcctgaac gtattgcaca gtttcatcaa gggctctttg cgcaattcct
     1261 aaggcctggg ccgcaattcc aattctaccc ccatcaagag ttgacatcgc aattttaaag
     1321 cccattcctt ctttgcccaa taaattgtca gccggtatcc ggcagtcccg aaagactagt
     1381 tctcttgtat cagaggcatt aatccccatc ttctcttctt ttttaccgag agaaaagccg
     1441 gcggtgtcgg cttctactat aaaggagcta atccctcgtg ttcccttgct cttatcggtc
     1501 atcgcatgaa taatgaagat atcggctgcc cctgcattgg taatgaagat cttagtacca
     1561 tttaaaatat attcatctcc ttctctcaca gcagtagttt taactcccgc tgcatcggtg
     1621 cctgcccccg cttctgttaa agcaaaggct cctaactttt caccctttgc aagtggtttt
     1681 aaatattttt ctttctgtgc ttcggttcca tatttataga tgggatgggc ccctaaagaa
     1741 gtatgggcgg ataaaataac tccatgagta gcacaagcgt aagatagctc ttctactgcc
     1801 aaaatatagt ttaaggtatc agaaccggct cccccatatt tttgtgggaa agggatcccc
     1861 atcatgtcag cctctgccat cttcttaata ttatcagccg gaaattcaca gttttcatcg
     1921 atctcagctg ctatcggctt aatttcattt tcagcaaatt ctttataaac ttttttacac
     1981 atctcttttt cttcgcttaa attgaaatcc attatatata cctccatatg taaaaatatt
     2041 cacaatatat tgcaaaattt tttattcccc ttttacagtt ggcatcaatt ctgcctgccc
     2101 tgttaggaca acttcttctt tttgattggt gcaaaccgta tttagagtta ctctattttt
     2161 ttcctcatta atatcttcta cttcagcggt cgcagtaact gtatccccaa agcgaaccgg
     2221 ggcagtgaat ttaagatcct gtgcaacata tacactaccg ggaccaggta attgtgtacc
     2281 caatactgtt gaaatcaaac cggctgctat cataccgtga gcaatcctgc ctttaaacat
     2341 ggtctcttta gcgaaactct cattaacatg agccggatta aaatcccccg ttatccccgc
     2401 gtaattatag acatcagttt ctgtaattgt tttagtaaat gaagcctttt ctcctatctc
     2461 tagttcattg actgttttcc ctctatccat agtattacct ccataagtga ttattatttg
     2521 caattacttg ttaataatat cacaaaggta tattctatgc aaccttcagc aagcatttaa
     2581 tctcatatga ctttagttga aaaaatataa ctctgcccta gtttttgaga agcagagttt
     2641 caatacttac ttatattata tttttaattt tttcaagcat tttcaatgag catattcatt
     2701 gtttcatagg caaaatcaac tgctgccaga tatttactat atagtttttt aggattcaat
     2761 ccatatgttt tttcgatgat ggaaatttcc tgccatttta ccctttcaaa ggcaattgcc
     2821 agctgcagga tttctccata tataccctct cctcttaaaa gggcctcttt tatatctccc
     2881 tttaaggaaa tttcatcaat gatttcttct aaaggcttat ttaagaaagc gtccatcatt
     2941 gacagcatac ccatagtaaa aagttcactg ctcttttctt ttaccccaaa atcttctgcc
     3001 gccgattcga caaactttgc cctggttaag gtattgacaa ataggatatc cggtttatca
     3061 tttcttaacc cttctgctag atatattaga ctccattttt ttaaaccatt tatacctaaa
     3121 agggtggaag cctgtttgat agaagataca ttataaccgt aataggcaga attgatggta
     3181 cttaagagac tgacagtcat cgacatatcg tttttaatga tatcctctaa tttatcaaaa
     3241 tcaatttcgg gtttatttaa ttcttttaag aaatttagat aattcagctt ataaccgggg
     3301 atttttctat tggccataac ttccggcttg gtaaaaaata tgccctgaaa atagtcataa
     3361 ccgcattcct tagcctgatc aaattgttca tgttcattaa tattttcggc catgaatttt
     3421 aagtttttat tatacttatt tttaagtaga tttattatca aagtatgttc cttttcttta
     3481 aacttattaa aatctagttt taggatatct gcatacttaa ttaacgtagt gtttggttta
     3541 taggggttta gatcatttag gatgataagg gcaccttcat cctttaattt aacaatattg
     3601 cttattaggt ttgcatcata atcaatatct tcagaaatct ctatcccgag gttatccttg
     3661 gctaaaaaat taacaacttc attttttaaa agatccctgg taaaattcat gaaaatcttc
     3721 ttgccattag aaagatttgt ctgttcgata aattccacat cattgttaat tgcttcagct
     3781 gttatatcaa tattatcatc agtgatttta ttatcgtacg aatttttaaa aatgatttca
     3841 taagctatta catcttgatt taaattaagg accggttttc ttgctaaaat agactcttcc
     3901 attttaatca accctccctg tttaacttat tattatgcaa aataaaaaat atatattatt
     3961 aatataaaaa aactatgact gcacagccat agattactgt tttaatggta acctggcagt
     4021 cttatattga agacccatag ctttgcgccc cagcttcaca gccggtttgc ctttttcata
     4081 atttatttaa ctctaatttt actattcaaa aattacttac ctaaa
//
LOCUS       DATGCJ010000193        17204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_607111, whole genome shotgun sequence.
ACCESSION   DATGCJ010000193 DATGCJ010000000
VERSION     DATGCJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607111"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1010
                     /locus_tag="VKN64_08915"
     CDS             363..1010
                     /locus_tag="VKN64_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMA60399.1"
                     /translation="MPKINEDEQQQIRELFDGKMENEVYIQFFTSDDEECQYCDDTEE
                     ILDELKNLHDKINLEKNELGNGNTEKYNIDRVPAILFTDKEGNDSGVHFYGIPSGHEF
                     NTLIEGIISMSNGQTELSDDVIEELKEIDNDVLLQVFITPTCPYCPRAVKVAHEMAMH
                     NPKIKGEMVEAIEFPDLSSKYEVSGVPKTVINSGKAEQVGAVPAKTILDKIKTAI"
     gene            1061..1384
                     /gene="trxA"
                     /locus_tag="VKN64_08920"
     CDS             1061..1384
                     /gene="trxA"
                     /locus_tag="VKN64_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMA60400.1"
                     /translation="MSKKPVEVNDGNFRREVLRSDVPVLVDFWADWCAPCKMVAPVVE
                     KIAREYSEDIKVAKLNVDENQSTASRYDVMSIPTLVIFEDGKEKDRVIGYAPKNKLIS
                     KLGLR"
     gene            1541..1948
                     /locus_tag="VKN64_08925"
     CDS             1541..1948
                     /locus_tag="VKN64_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMA60401.1"
                     /translation="MQRVLIIYSKEGDVKEIAEGIAEGAREKGYKVDLQDTEITGKPV
                     SFYPYDVVIAGAPTQGIFRGKIPSDLASFLKECKRTGGQAAMAFVTPRFFATNKALKL
                     VMGELEKLGCIVKDFRTIKNRKEAVDFGKALKL"
     gene            2020..2451
                     /locus_tag="VKN64_08930"
     CDS             2020..2451
                     /locus_tag="VKN64_08930"
                     /inference="COORDINATES: protein motif:HMM:NF020711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HMA60402.1"
                     /translation="MEEVMIKVNNKQEFTDGKKDEISYEGKGEYKYKNGKHYLIYEET
                     NQGLEEVKTIIRFNEKINRILIKRSSPNEMRQVFDVKENCDFLYNLGSHKMRFETDTK
                     KLQIETGPQRGEISIEYNLIQNEQVFTSNNLKIEYRFIDKE"
     gene            2460..4124
                     /gene="argS"
                     /locus_tag="VKN64_08935"
     CDS             2460..4124
                     /gene="argS"
                     /locus_tag="VKN64_08935"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMA60403.1"
                     /translation="MEKNMIEKVENKLRAAIKEGLVKTLEQADITLEQIPDIKVEVPR
                     EKEHGDYATNIAMVMAKHFKRAPRQIAELLKENIEADLIKDISVAGPGFINFNLENSW
                     LYETIDLIDKLGKDYGKVDVGDHRRVLIEFVSANPTGPLHVGHSRGAVVGDMTANIME
                     AAGYEVDREYYFNDAGNQIDLLAKSTLIRYKQLFDIEIDLPKNAYHGKYLIDIAKKLK
                     EKYGKSLLDKKEEEQLDICKEFAYESLKTKIEEDLKYFGIEFDNWFYESSLHEGDIED
                     AINILKEKGYTYEEEGALWLRSTEFDDDKDRVIVKSDGSLTYLAADIAYHLDKIERGY
                     DKLINVWGADHHGYIDRVKASIQALGYPKDILEIIIVQLVNLMRDGEKIPMSKRAGEF
                     VTMTDVIDEVGKDAARYFYIMRSSDSHFDFDLELAKEESSSNPVYYIQYAHARIHSIL
                     ENAEDIDLKDVDYKLLDSEEEIDLIKLMARYPQVIKDSAESREPHILANYAYELANAF
                     HIFYNKRQVISSDESLSKSRLKLVVSLQQVLKNLCRVLGIEAPDQM"
     gene            4155..5762
                     /locus_tag="VKN64_08940"
     CDS             4155..5762
                     /locus_tag="VKN64_08940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMA60404.1"
                     /translation="MTKYIFVTGGVVSALGKGITAASLGRLLKSRGLNVSIQKFDPYI
                     NVDPGTMSPYQHGEVFVTRDGAETDLDLGHYERFINEDLTSNNNVTTGQIYGSVIKKE
                     RRGDYLGATVQVIPHITDEIKERITRVGKETDADVVITEIGGTVGDIESQPFMEAIRQ
                     LKGDLGKNRVFYLHCTLVPYVKTAGELKTKPTQHSLKELRGIGIQPDSLICRSDRELT
                     KEVKDKISLFGDIEKKAVIQVVDADHIYEVPLMIEKEGLAEIVIEKLELEDDVKKPDL
                     NNWKDMVKKMKNLSNEIKVAIVGKYVELPDAYISISEALSHAGVANDSEVNIEYIYSE
                     DLEKGEDLKEVFEGMDGIIVPGGFGHRGIEGKIEAIRYARENKIPYLGLCLGMQCAVI
                     EYARNVLNLAGANSSEFDPDTDHPVIALMPEQKDIEDMGGTMRLGIYPCRLTEGTDSK
                     ELYEEEIIYERHRHRYELNNEYRNELKNNGMAMAGISPDEKLVEIVEVKDHPWFVGTQ
                     FHPEFKSRPNRPHPLFKGFVKAALNYK"
     gene            5796..6563
                     /locus_tag="VKN64_08945"
     CDS             5796..6563
                     /locus_tag="VKN64_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMA60405.1"
                     /translation="MNNNYVEVDYAPPIATVFLSRPDVLNAYNEKLLDQLYSKSKELF
                     NDDDIKAVIITGRGAKSFTVGADLNLLEDLDSETAGVIAKKGKAICDLIEETEKVVIA
                     AVNGHALGGGMEITLACDLRVASTRARFGQPEVTLGLIPGFSGTQRLPRILGIGRAKE
                     LLFTGNIIDAEEAFELGLVNKLVTPRDLLKVSKSMAKRITDQSSKAVGMIKKAINYGY
                     SHGCDKGKEFETDSFKECFKDQEHTERINRMKENLKD"
     gene            6574..7290
                     /locus_tag="VKN64_08950"
     CDS             6574..7290
                     /locus_tag="VKN64_08950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMA60406.1"
                     /translation="MEGKRTILFDLDGTLLPIDMDKFIEKYFRLLSGHFADLYNPEYF
                     VNIINKATENMIRNDGQQTNKEAFEEKFFELIDLNGVGEKDIWDRFYDFYDNLFPTLK
                     KHFELDRLGYEIVEAAKAKDFNMVIATNPLFPRNAITTRLKWIDLNPADFKYITSYEE
                     MHYCKPNINYYREILDKTNYQAESCVMVGNDMRDDMVAKKVGLKTFLLNDFKVEREGT
                     SIKPDWQGTRIEIIDYIKKL"
     gene            7302..7676
                     /locus_tag="VKN64_08955"
     CDS             7302..7676
                     /locus_tag="VKN64_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMA60407.1"
                     /translation="MKNIINTDKAPAAIGSYSQAVKAGNTVYISGQIPLNLEGELIMT
                     SIEAQTEQVLKNLRAILKEAGGDFENVVKATVYTDDISYFNEINEVYGKYFAEEAPAR
                     AFVEVAALPKGVNVEIEAIAVI"
     gene            7803..8459
                     /locus_tag="VKN64_08960"
     CDS             7803..8459
                     /locus_tag="VKN64_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60408.1"
                     /translation="MLIDKTIEIALRCHHCGRLEKTKLNIFQVRDDHLLDIKCSCGST
                     KARIKAKSDKYVHFEYNCFLCDEYHRRVIEKDEFWSTGSLKHLKCLNTGLEIGYYGAP
                     HLIDEELNKQKEELKILADELGLNDYQEPELLLEVFNIVHDRASLSKLYCECGSDDID
                     VKLYSSKIELKCRECGSVLNISAETEEQLNEIKNKGDIVIKSMNGPDNNWSDNKYKGE
                     "
     gene            8466..9320
                     /locus_tag="VKN64_08965"
     CDS             8466..9320
                     /locus_tag="VKN64_08965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HMA60409.1"
                     /translation="MGLVPMADILNKANEEYYAVGGFNINNMEFLQGIIWAAEEANSP
                     LILQASEGAIRYIGMDYVINMVKAAVKDTHIPVALHLDHGSSYEVIMRCIREGFSSVM
                     FDGSKYPFEENIRLTQEVVKAAHSVGVTVEAELGKIGGSEDDVEVDEKDAMFTKPAEA
                     EEFVEKTEVDALAIAIGTAHGVYKGKPELDFDRLEEIASILDMPLVLHGASGVPEEDI
                     RKAVKLGINKVNVNTAFQQAFTAKIRELFDKDPDLYDPRKYCGPGRDAISAAVKEKIE
                     MLGSKNKA"
     gene            9428..10441
                     /locus_tag="VKN64_08970"
     CDS             9428..10441
                     /locus_tag="VKN64_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HMA60410.1"
                     /translation="MLNIWIEFIIMAGLIIVSGTYLSRYGDVISEKTGLSQAVIGVVL
                     VAMATSLPELVTSSTSAIIDAPNIAVGNAFGSNTFNLLILGIIDVMHENRLPLSFSLN
                     YSHVMTGTWGILLSAIVVISIIIANFTEYSFGIFGIGLDAIVLIGTYLLAMFLIIRFE
                     NKNPLDDNIREEVPYLDTTLKKASIGFTICAAIIVYAGVRLAITGDKIATLTGIDQTF
                     IGSILVAAATSLPELVTAISAVKIKAYDMAAGNVLGSNIFNMSIIFFADLFYRNGYIL
                     SSVRMLHLITASIGIIMASIFLVGLFYKSKRTFLGMGWDSIAVFIVYLLGFYLLFTLG
                     ISI"
     gene            10469..11731
                     /gene="rho"
                     /locus_tag="VKN64_08975"
     CDS             10469..11731
                     /gene="rho"
                     /locus_tag="VKN64_08975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMA60411.1"
                     /translation="MLNISDLEKKTITELHDLAKDLDISGYTTMRKKDLIFAILKNET
                     EEGGNIFAEGVLDIIPNEGYGFLRPSKYVPSSDDIYISASQIRRFDLRSGDVVSGQVR
                     EPKENEKYFALLRIEAVNYQDPEKATERAYFEDLTPLYPNERLRLEYNQQDVSTRLMD
                     LIAPIGKGQRGLIVSPPKAGKTVLLKKIANSIRHNYPNVRLMILLIDERPEEVTDMSR
                     SVDAEVISSTFDEAPEQHLQVAKLVLKKAKRLVEHKYDVVILLDSITRLARASNITVP
                     ASGRTLSGGLDPSAMHFPKKFFGAGRNIEEGGSLSILSTALVETGSRMDDVIYEEFKG
                     TGNMELHLSRELAERRIFPAIDIQKSGTRKEELILDEEELEVMWGLRKQIAQSRPYDI
                     METFIKQIKNTKDNKELLTALQQIIKNN"
     gene            11894..12118
                     /gene="rpmE"
                     /locus_tag="VKN64_08980"
     CDS             11894..12118
                     /gene="rpmE"
                     /locus_tag="VKN64_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HMA60412.1"
                     /translation="MQNELHPEFKETTITCACGEEYKTKSTRENIRVEVCSSCHPFYT
                     GKKRRAAKGGRVERFNKKYGFSEEEETEEE"
     gene            12207..12788
                     /locus_tag="VKN64_08985"
     CDS             12207..12788
                     /locus_tag="VKN64_08985"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HMA60413.1"
                     /translation="MYKINNSGWIEVITGPMYSGKSEELIRRIRRVKIAKKKVKVFKP
                     LMDDRYSNGNVVSHSGDSIEAIPVDHPEELYERLEDDVEVVAIDEIQFFSEEIVDICE
                     TLADKGKRVVVAGLDRDFRNEPFVPMPELLSRAEYVEKLHAICLQCGDPATRTQRLID
                     GEPASYNDPIILVGATENYEARCRSCHEVKDKP"
     gene            12800..13687
                     /locus_tag="VKN64_08990"
     CDS             12800..13687
                     /locus_tag="VKN64_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HMA60414.1"
                     /translation="MNKDVDYGGQAVIEGVMMKGLNKLAIAVRKPDNSIVVKKRNLTP
                     LSDKFKVFGWPFIRGVVALFSSLILGMQSLTFAANQVTEEDEELTPLEMGLTILVSLG
                     LAILLFVALPAGIIALIQEYIEYNVVLNLIEGLIKVSAFLLYIVAISRLDDIKRVFMY
                     HGAEHKVIHNYESGKELSVENAREFTTMHARCGTNFIFIVIILSILFFSFFGRPPLLY
                     RILYHLMLLPVIAGTSYEVIKLAGGEEVNPVIKVLATPGLWLQKLTTSEPEDDMLEVA
                     ITALKGVLPEEERGEAENV"
     gene            13680..14750
                     /gene="prfA"
                     /locus_tag="VKN64_08995"
     CDS             13680..14750
                     /gene="prfA"
                     /locus_tag="VKN64_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMA60415.1"
                     /translation="MFDLEEKLEEKYEEYQKLNKKISDPEVMNDSNKYQKLLKRHAKL
                     KELIEYYKEYKEIKEGIEEAEEIIEEDDSEMEELAEMELAELKPRLEKLENKLPLMLL
                     PENPDDEKNVIVEIRAGAGGDEAGLFAADLFRMYTRYAEDNSWKWELMNANESGVGGF
                     KEVIFSIEGKEVYKKLKYESGVHRVQRVPSTESSGRIHTSTATVAVLPEAEEVDIEID
                     TNDLKIDFYRSSGPGGQSVNTTDSAVRITHEPSGLTVSCQDEKSQHKNKAKAMRVLRA
                     RLKEKREKEKQEERAEARKSQIGTGERSEKIRTYNFPQGRVSDHRINLTEHKLEQILD
                     GDLDIIIEPLIEEDTMKKLEKV"
     gene            14754..15611
                     /gene="prmC"
                     /locus_tag="VKN64_09000"
     CDS             14754..15611
                     /gene="prmC"
                     /locus_tag="VKN64_09000"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMA60416.1"
                     /translation="MNIKEVLVNTEEYFQKNGIESARLDAEVLLAELLDMERIKLYVN
                     FDYPLNDEEISQYREMIKKRAQHIPVAYIIGHKEFMSLDFYVNKNVLLPRPETEILVE
                     YLIDCFQEKEMDTVNIVEVGTGSGAIMVSLGNYLDNARILGVEIEEGALEVTRKNIER
                     FSLDDRLKVTKGDLLKPLIKREIKNVDLVVSNPPYISEEEMDKLPPEVKKEPAKALYG
                     GRAGLDIYKDLIPQSRQVLKDNGMLALEIGYQQAEAVKNILVKNNFKDIDVRKDYADK
                     DRFIIAYKG"
     gene            15616..16680
                     /locus_tag="VKN64_09005"
     CDS             15616..16680
                     /locus_tag="VKN64_09005"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMA60417.1"
                     /translation="MYKTKVKKIAQNLIRKNKVDVIKKSFAIKEAAEYILADEVVAFP
                     TETVYGLGANALKEEAVSKIFKAKGRPSDNPLIAHIASKDQLPALIEGEIPAAAEKLI
                     ERFWPGPLTIIFKKSKKVPKITTAGLDTVAVRMPSHPVALALIEKVNLPLAAPSANSS
                     GFPSPTKAEHVYNDLNGKIPLIVDGGICNIGVESTVIEIEGNNVSILRPGGTPKELIK
                     KTLGDKYQVETLNQAEVKSPKSPGMKYKHYAPHTPLWIIQAGNIENVIQQNDVNNENT
                     LLILSSETLRGLEQDSLRVKIQDMGSIEDLEHIAKHLYNILRETDEQDFELVLIEEVP
                     DKEIGEAVMNRLKKAAVKII"
     gene            16695..17144
                     /locus_tag="VKN64_09010"
     CDS             16695..17144
                     /locus_tag="VKN64_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HMA60418.1"
                     /translation="MKILFVCTGNTCRSPMAEYFAKQYIQENDLEDFEVNSAGINAVT
                     GEKASSEAIEVMKEKDIDITGHKSKMLMEADLKESDRVFAMTNSHKEVIEDMYGYEEV
                     YTLKHFVQSCEDCDILDPYGYDIDKYRKTRDEIEYAVKELLKKLSNS"
BASE COUNT         5904 a         2679 c         3811 g         4810 t
ORIGIN      
        1 ttaaaagtcc gttgtacatc tatgtataaa gcgatcggat ataacataaa acagctggtt
       61 aaaatactaa atggaggttt tagaagttgt ttgagtacat aaaccaccaa aagtaggaga
      121 attataactc agacaacatc agtatagaaa attaatttca tttaactggt ttgaaatatt
      181 cattggaaaa tatattttgc tcaagttagt ttatttgagt tctaagttgg tctattataa
      241 tatttttcgc agtgcaatca ttgattaatt gtaaaatatt taactttaat tttctgagat
      301 tcttgtataa tagtatatgt cgaaagttga aataagcaaa ttatctaaaa ggagtggtct
      361 tagtgcctaa aattaatgaa gatgaacaac aacagatcag agaattattt gatggaaaga
      421 tggaaaatga agtatatatt cagttcttta catctgatga tgaagaatgt cagtactgtg
      481 atgatactga agaaatctta gacgaactta agaacttaca cgataaaatt aatcttgaga
      541 agaatgaact aggcaatggc aatactgaaa agtataatat agatagagta cctgccatcc
      601 tatttacaga taaagaaggc aatgactcag gcgtacattt ttatggtatc cccagtggtc
      661 atgaatttaa tacccttatt gaaggtatta taagtatgtc caatggtcag actgagcttt
      721 cagatgatgt tatcgaagaa ttaaaagaga ttgataatga tgttttactt caggtattta
      781 ttactccaac ttgtccttat tgtccaagag cggtaaaagt agctcatgaa atggcaatgc
      841 ataatcctaa gatcaaaggt gaaatggtgg aagcgattga atttcctgat ctctcaagca
      901 agtatgaagt atcaggtgta ccaaagacag ttataaatag tggtaaggca gaacaagttg
      961 gtgcagttcc tgccaaaact attttagata aaattaaaac agctatttaa gtaattatta
     1021 tttaatctta ttataatttt ttaaggaggt ttctaacaag atgagcaaaa aacctgtaga
     1081 agtaaatgat ggaaacttta gaagagaggt tttaaggtca gatgtacctg ttttagttga
     1141 cttttgggct gactggtgtg caccatgtaa aatggtggct cccgtagtcg aaaagatcgc
     1201 cagggaatat tctgaagata tcaaagttgc caaacttaat gttgatgaaa accaatctac
     1261 agcttccaga tatgatgtta tgagtattcc aaccctcgtt atctttgaag atggaaaaga
     1321 aaaagatagg gtaattggat atgctcctaa aaacaagcta atctcaaaac ttggtttaag
     1381 ataattaaat aagtaataaa aaatagcagg ggataccctg ctttttttat tttcacccat
     1441 aaaaattttc tatatttagt gaaaaatttt attagttatg atataattct tttgtataat
     1501 atgcaggata ttttttataa aatttctgag gggtggtatt ttgcaaagag tccttattat
     1561 ttattcaaaa gaaggtgacg ttaaggagat agccgaagga attgcagagg gcgccaggga
     1621 aaagggatat aaagtagact tacaggatac agaaatcaca gggaaacctg tctcctttta
     1681 tccatatgat gtggtaatcg caggtgcgcc gactcaaggt atattccggg gtaaaatccc
     1741 ttcagattta gcgagttttt taaaagaatg taaaagaacg ggtggacagg ctgcaatggc
     1801 atttgttaca ccccgctttt ttgcgaccaa taaagccttg aaactagtaa tgggcgaact
     1861 ggaaaaatta ggttgtatcg taaaagattt taggacaata aaaaatagga aagaagccgt
     1921 tgactttggt aaggctttaa aactataatt atatacataa atgccacagt gaaaactgtg
     1981 gcttttttgg aattataaaa atagttgagg aatgatataa tggaagaagt aatgattaaa
     2041 gtaaataaca agcaggaatt tacggatgga aaaaaagatg aaataagtta tgaaggtaag
     2101 ggagagtata agtacaaaaa cggcaaacat tacctcatat atgaagagac aaaccagggg
     2161 cttgaagaag tcaaaaccat tatccgtttt aatgaaaaaa taaataggat acttataaag
     2221 agatcgagtc ccaatgagat gagacaggta tttgatgtta aagaaaactg tgatttttta
     2281 tataacctgg gcagtcataa gatgaggttt gaaactgata ctaaaaaact tcagatagaa
     2341 actggtcccc aaagaggcga aatttctata gaatataatc taattcaaaa cgagcaagtc
     2401 tttacttcta ataatttaaa gatcgaatac aggtttattg ataaggagtg acagatatta
     2461 tggaaaagaa tatgattgaa aaagtagaaa ataaattacg tgcggctatc aaggaaggtt
     2521 tagttaaaac cttagaacag gcagatatta ctttagaaca gattcctgat attaaggtag
     2581 aagttccccg tgaaaaggaa catggggact atgcgactaa tattgccatg gtaatggcca
     2641 aacattttaa gagagcaccc cgccagattg cagaactgct caaagaaaat attgaggctg
     2701 atttaattaa agatatttct gtagccggac caggtttcat caacttcaac ctagaaaata
     2761 gctggttata tgaaacgatc gatttaatag ataaattagg aaaagattat ggaaaggttg
     2821 atgtgggaga tcatagaaga gtcctcattg aattcgtaag tgccaatccg accggacctt
     2881 tacatgtcgg ccacagccgg ggggcggttg taggagatat gacagccaat ataatggagg
     2941 cagccggtta tgaggtagac cgcgaatact attttaatga tgccggtaac cagatagatc
     3001 tcttagcgaa gtctacctta attagatata aacagctttt tgatatcgag atagacttac
     3061 ctaaaaatgc ttatcacggt aagtatttga tcgatattgc taaaaagctt aaagagaaat
     3121 acggcaaaag ccttttagat aaaaaagaag aagagcagtt agatatttgt aaagagtttg
     3181 cttatgaaag tttaaagact aaaattgaag aagatttaaa gtatttcggt attgagtttg
     3241 ataactggtt ttatgagagc tccttacatg agggagatat agaagatgca atcaatattt
     3301 taaaagaaaa gggctatacc tatgaagaag aaggagcttt atggttaaga tcaactgaat
     3361 ttgatgatga taaggaccgg gtgatagtca aatcagatgg ttccttaaca tatttagccg
     3421 cagatattgc ctaccattta gataagattg aaaggggtta tgataagtta attaatgtct
     3481 ggggtgccga ccaccacggc tatattgata gggtgaaggc ttccattcag gcgcttggtt
     3541 atcccaagga tattttggaa ataataatag tacaactcgt aaatttaatg cgagatggag
     3601 aaaaaatacc gatgtccaaa agggccggtg aatttgtgac catgaccgat gtaattgatg
     3661 aggttggtaa agatgctgcc cgttacttct atatcatgcg cagttcagac agccacttcg
     3721 acttcgattt ggaattagcc aaagaagagt caagcagcaa ccccgtctat tacatccagt
     3781 acgcccatgc ccgcatccac agcattctcg aaaatgctga agatatagat ttaaaagatg
     3841 tagattacaa actgttggat tcggaagaag agattgactt gatcaagtta atggcgcgtt
     3901 atccccaggt aattaaggat agtgccgaaa gcagagaacc ccatatttta gcaaactatg
     3961 cttatgaatt agccaatgct ttccacattt tctataacaa gagacaggta atttcaagtg
     4021 acgaaagttt aagtaaatct agattgaagc tggttgtttc cttacagcag gtacttaaga
     4081 acttatgtag agtcctcggt attgaagcac cagaccagat gtaaataata ttttcaagaa
     4141 aggagttgtc agctgtgaca aaatatattt tcgtaacagg gggagttgtt tctgccctgg
     4201 gcaagggaat tacggccgcc tctttgggtc ggttgttaaa gagccgcggg ttaaatgtga
     4261 gtatccaaaa gtttgacccc tacataaatg tagatcccgg cacgatgagc ccctatcagc
     4321 acggtgaagt tttcgtgaca agagatggag cagaaacgga cttggattta gggcattatg
     4381 agagatttat aaatgaagat ctaaccagca ataataatgt tactaccgga caaatatatg
     4441 gttcagttat taaaaaagag agaagaggtg actacctggg tgccaccgtt caggtaatcc
     4501 ctcacataac tgatgagatt aaagaaagaa tcacccgtgt gggtaaagag accgacgctg
     4561 atgtagtgat aactgaaata gggggtacag ttggagatat tgaaagtcag ccctttatgg
     4621 aggcaatcag acagcttaag ggcgaccttg gtaaaaatag agtattttat ttacactgta
     4681 ctttagttcc ttatgtaaag acggccggag aattaaagac caaacctact caacatagtt
     4741 taaaagaatt aaggggaatt ggtattcaac ctgattctct catctgtcgt tccgatcgag
     4801 agttaaccaa agaagtaaaa gacaaaatat cactctttgg agatatagaa aagaaggctg
     4861 ttatccaggt agtggatgcc gaccatatct atgaagtacc tctcatgatc gaaaaagaag
     4921 gcttggccga gatagttatc gagaaacttg aattggaaga tgatgttaaa aaaccagacc
     4981 ttaacaattg gaaagacatg gttaaaaaaa tgaaaaactt aagtaatgag attaaggtag
     5041 cgatcgtggg taagtatgtt gaactacccg atgcctatat cagtatcagt gaggccttaa
     5101 gccatgccgg ggtagcaaat gatagtgaag taaatataga atatatatat tcagaggatt
     5161 tagaaaaggg cgaggaccta aaagaagtat ttgaagggat ggatggtatt atagtaccgg
     5221 gtggttttgg ccaccgcggt atagaaggca aaattgaagc aatcaggtat gctcgcgaaa
     5281 acaagatacc atacttagga ctctgtcttg ggatgcagtg tgccgtaatt gaatatgccc
     5341 gcaatgtgtt gaacttagca ggtgcaaaca gttctgaatt tgatcctgac acagatcacc
     5401 ctgtaatcgc cttaatgcct gaacaaaagg atattgaaga tatgggcggc acgatgcggc
     5461 tcggtatcta tccctgccgt ttaaccgagg gaacagacag taaagagctg tatgaggaag
     5521 aaataatata tgaaagacac cgccaccgtt atgagttaaa taacgagtac cgcaatgaat
     5581 taaaaaataa tggtatggcg atggccggta tttcaccgga cgagaagttg gttgagatcg
     5641 tagaagtaaa agatcatccc tggtttgtcg ggacacagtt ccatcctgaa tttaaatcaa
     5701 gacccaatag gccgcatcct ctttttaaag gatttgtcaa ggcggcttta aattataaat
     5761 agtgagagcg caaacagacc atgaggaggt ataccatgaa taacaattat gtggaggttg
     5821 attacgctcc tcccatagcc acggtttttt tatcccggcc cgatgtactc aatgcctata
     5881 atgaaaagct attagaccaa ttgtatagta aaagtaaaga actatttaat gacgatgata
     5941 ttaaagcggt tatcattaca gggcggggag ccaagtcctt tacagtaggg gcggacttaa
     6001 acttattaga agatttagat tccgaaactg ccggtgtaat tgccaaaaag gggaaagcaa
     6061 tctgtgattt aattgaagag actgaaaaag tagttattgc agcagtcaac ggtcatgccc
     6121 ttgggggagg tatggaaatt actttagcct gtgatttaag ggttgccagc accagagcta
     6181 gatttggtca gcccgaggtt accttaggct taatccccgg gttttcaggt acccagagat
     6241 taccccgtat tttgggaatc ggtagggcga aagaacttct ctttaccggt aatataattg
     6301 atgcggaaga ggcgtttgaa ctgggtttag tcaataagtt ggttactccc cgggacctac
     6361 ttaaagtatc aaaaagtatg gccaaacgaa ttacagatca gtcttccaaa gcggttggaa
     6421 tgataaaaaa agcgattaac tacggttatt cgcacggttg tgataagggt aaagaatttg
     6481 aaaccgattc atttaaagaa tgttttaaag accaagaaca tacagaaaga ataaatagga
     6541 tgaaggaaaa tcttaaagat taggtgataa agcatggaag ggaaaaggac aattttattt
     6601 gacttagatg gaacattatt accgattgat atggataaat ttatagaaaa atactttcgt
     6661 ttactttctg gtcattttgc agatttatat aatccagagt actttgtaaa tattatcaac
     6721 aaggcaacag aaaacatgat cagaaatgac ggccagcaga ccaacaaaga ggcttttgaa
     6781 gagaagttct ttgaattaat tgatttaaat ggagtaggag aaaaagatat ctgggataga
     6841 ttttatgatt tttatgataa tcttttcccc actttaaaaa aacatttcga acttgatagg
     6901 ttagggtatg agatagtgga agcggccaaa gccaaggatt ttaatatggt tattgcaaca
     6961 aatccacttt ttccccgtaa tgcaataaca actagattaa agtggattga tttaaacccc
     7021 gcggacttta aatatattac ttcctatgag gagatgcatt actgcaagcc gaatattaac
     7081 tattacaggg aaattctcga taagactaac tatcaggcgg agtcctgtgt aatggtgggt
     7141 aatgacatgc gggatgacat ggtagcgaaa aaggttggac ttaaaacatt tttactgaat
     7201 gattttaagg tagaaagaga gggtacctct ataaaaccag attggcaggg aacgcgtatt
     7261 gaaataatag attatataaa aaaattatga ggtgataaaa aatgaaaaat attatcaaca
     7321 ccgataaggc accggctgct atcggtagtt attctcaggc agtgaaagcc gggaatacag
     7381 tttatatcag tggtcagatc cctttaaatt tagaaggaga actgattatg acttcgattg
     7441 aagcccagac tgaacaggtc ttaaagaatt taagggcgat ccttaaagaa gccggcggtg
     7501 actttgaaaa cgtcgtgaag gctacagtct atacagatga catatcatat tttaatgaga
     7561 tcaatgaagt ttatggaaag tattttgcgg aagaagcacc ggcccgggcc tttgtggaag
     7621 ttgcagccct accaaaggga gttaatgtag aaatagaggc aattgctgta atttaaggaa
     7681 aaaagtaaaa gctttccccc tctgaagatg gtatttttaa gttataatgg aggattttta
     7741 atttcattag cgaattaata atctgagggg gtgagccctg ttaactttta acaggaatac
     7801 ttatgttaat cgacaagacg attgaaatag ctttacgctg tcaccactgc ggccggctag
     7861 agaaaacaaa actaaatatt tttcaggttc gtgatgatca tttacttgat ataaaatgct
     7921 cctgtggtag cacaaaagca aggataaaag caaaaagtga taaatatgtt cattttgaat
     7981 ataactgctt tttgtgtgat gaataccatc gtagagttat agaaaaagat gaattctggt
     8041 ctactggttc cttaaaacac ctaaagtgtt taaatacagg cttagaaatt gggtactacg
     8101 gcgccccaca cctaatcgat gaagaactca ataaacaaaa agaagaatta aaaattctag
     8161 ctgatgaact cggcttgaat gattatcagg aacccgaatt attactggaa gtgttcaata
     8221 ttgtacatga tcgggcttca ctctcaaaac tatactgcga atgcggcagt gatgatattg
     8281 atgtcaaact ttattcgagc aagatcgaac tgaagtgtcg agaatgtgga agcgtgctca
     8341 atatttcagc tgaaacggaa gaacaactta atgagataaa aaataaagga gatatagtaa
     8401 taaaaagtat gaatggacct gataataatt ggtctgataa caagtataag ggggaataat
     8461 aaagtatggg tttagtacct atggctgaca ttttaaataa agcaaatgaa gagtattatg
     8521 ctgttggtgg ttttaatata aacaacatgg aatttttaca gggtattatc tgggcagctg
     8581 aagaggcaaa ttcacctttg attttacagg caagtgaagg tgcgataaga tacataggaa
     8641 tggactatgt tataaacatg gttaaagcgg ctgttaagga tactcatatt cctgtagcct
     8701 tacatttaga ccacggcagc agttatgaag taattatgag atgtattaga gaagggttta
     8761 gttctgtcat gtttgatggg tctaaatatc cctttgaaga gaatattagg ttaacccagg
     8821 aagtagtaaa agcggcccat agtgtgggag tgacagttga agctgaactt ggtaaaattg
     8881 gcggttcaga agatgatgtg gaagtagatg aaaaagacgc catgtttacc aaaccagccg
     8941 aagcggaaga gtttgtagaa aaaactgaag ttgacgcctt agctatcgca ataggtacag
     9001 ctcatggtgt atacaagggg aaacccgagc ttgattttga ccgtttagaa gaaattgcat
     9061 ctatcttaga tatgccctta gtactgcatg gtgcttccgg agtgccggaa gaagatatta
     9121 gaaaagctgt taaacttggt atcaataagg tgaatgttaa tacagctttc cagcaggctt
     9181 ttactgctaa gattagagaa ttatttgaca aggatcctga tttatatgat ccgaggaagt
     9241 actgtggtcc gggccgtgac gctatctcag cagcggttaa agaaaagatc gaaatgttgg
     9301 gtagtaaaaa taaagcttaa aaatccttaa aaaatctcgg ggtatccagc tggatgcccc
     9361 tttaatcctt cctaattccc gccattttta tatatttcct gagatatttt actgaaagga
     9421 gtagtatatg ctaaacattt ggatagaatt tataataatg gctggcctga ttattgtatc
     9481 aggtacctat ctttctcgtt atggtgatgt catatcggaa aagacgggat taagccaggc
     9541 tgtcatcggt gtagtattgg ttgcaatggc gacttcctta ccggagttag tgactagttc
     9601 aacctctgct attatagacg cccccaatat tgcagttggt aatgcctttg gtagtaatac
     9661 ctttaaccta ctcattttag gaataataga tgtaatgcat gaaaaccgat taccgctttc
     9721 ttttagcctc aattattctc atgtgatgac cggtacctgg gggatattat tatcagcaat
     9781 tgtagttatc tctatcataa ttgctaactt taccgaatac agttttggaa tttttggaat
     9841 tggtctggat gcaatagttc taatcggtac ttatttactc gccatgtttt taattatcag
     9901 atttgagaac aagaatccct tagatgacaa tatcagagaa gaagttccct atttagatac
     9961 tactttaaaa aaagcttcga taggttttac aatttgtgca gcaattattg tatatgccgg
    10021 tgtgcgtttg gccatcacgg gtgataaaat tgctacttta acggggattg accagacttt
    10081 tatcggtagt attctggtgg cagcggccac ttctctaccg gaacttgtaa cagctatttc
    10141 agctgttaaa attaaggctt atgacatggc agccggtaat gtattgggca gtaatatctt
    10201 taatatgagt ataatatttt ttgctgatct cttttatagg aatggatata ttctttcctc
    10261 agtgagaatg ctgcatctaa ttactgcttc catcggtatt attatggctt ccatcttttt
    10321 agttggatta ttttataaat ctaaaaggac atttttagga atgggttggg actcgatcgc
    10381 ggtatttata gtatatttac taggatttta tcttttattt acccttggaa tttcaattta
    10441 aacttttaaa aaaagaggtg taatacattt gctaaatatt agtgatttag agaaaaagac
    10501 aattacagaa cttcatgatc tggccaaaga ccttgatatt tcaggctaca ctactatgcg
    10561 taaaaaagac ctgatctttg ccattttgaa aaatgagacc gaagaaggcg gcaatatctt
    10621 tgcggaaggt gttttggata taatacccaa tgaaggttat ggttttttaa ggccttccaa
    10681 gtatgtaccg tccagtgacg atatctatat ttccgcctcg caaatcaggc gttttgattt
    10741 aagatcaggg gatgtggttt caggtcaggt ccgggaaccg aaagaaaacg aaaagtactt
    10801 tgcactgctt agaatagaag cagtcaatta ccaggatccg gaaaaagcaa ctgaacgagc
    10861 ctattttgaa gacctaaccc ctctttaccc taatgagaga ttaagattgg agtacaatca
    10921 acaggatgtt tccactcgct taatggactt aatagctccg attggaaaag gtcagcgcgg
    10981 acttattgta tctccaccta aagctggtaa aacggtttta cttaaaaaga tagccaatag
    11041 tataaggcat aattatccaa atgtcaggct aatgatcctt ttaattgatg aaagaccgga
    11101 agaagttacc gacatgtctc gctcagtcga tgcagaagtt attagttcta cttttgatga
    11161 agctcctgaa cagcatcttc aagttgccaa actggtttta aagaaggcaa aaaggttggt
    11221 agagcataaa tatgatgttg tgattctttt ggatagtatc acaagattag ccagggcttc
    11281 caatatcacg gtaccagcca gtggacggac cctttccggg ggacttgatc cttctgccat
    11341 gcattttccc aaaaagtttt ttggggcagg tagaaatatt gaagagggcg gcagtttaag
    11401 tattctctcc actgctttgg ttgaaaccgg tagtaggatg gatgatgtga tctatgaaga
    11461 atttaaaggt accggtaaca tggagctcca cctcagccga gaactggctg aacggagaat
    11521 tttccccgct attgatattc aaaagtccgg caccagaaaa gaagaactta ttttagatga
    11581 ggaagaactg gaagtgatgt ggggactgag aaaacagatc gcccagtcgc ggccttatga
    11641 tataatggag actttcatta aacagattaa gaacactaaa gacaataagg aacttttaac
    11701 agctttacag caaattataa aaaataatta aaaatccttg ctatttcggc tttttttagg
    11761 tataatgtgg ggtggctgag atgaaggttg catagtaaat tttactatgt tataataatt
    11821 ctagctgtca agaagagatt aatattaatc atatataaca ggaataatga ctatttagaa
    11881 agaggtgaaa tatatgcaga acgagttaca tccagaattt aaagagacta ctattacctg
    11941 tgcttgtggt gaagaatata agacaaaatc cactagagaa aatattcgtg ttgaggtttg
    12001 ttcaagctgc catcctttct ataccggtaa gaaacgtaga gccgctaaag gtggtagagt
    12061 tgaaagattt aataagaaat atggtttttc tgaagaagaa gaaaccgaag aagaatagtt
    12121 attcttttaa caattatgca gggcccaagc tctgcttttt tcttatttaa ttaaaaaatt
    12181 aagtaatgag aggggagcat agttggatgt ataagattaa caacagcggc tggattgaag
    12241 ttattacagg acccatgtat tctggtaaaa gtgaagagct tatcagaagg atacgcaggg
    12301 ttaagattgc taaaaagaaa gtcaaggttt tcaaaccttt aatggatgat agatatagta
    12361 atggtaatgt tgtttctcac agtggggaca gcattgaagc aataccagtt gaccatccgg
    12421 aagaattgta tgaaagactg gaagatgatg tggaagttgt tgcaatcgac gagatccaat
    12481 ttttctcaga agagatcgtc gatatctgtg aaacacttgc cgataaaggc aaacgagttg
    12541 tcgtagccgg cttagaccga gattttagaa atgaaccatt tgttcccatg cccgaactac
    12601 tatcgagggc agagtatgta gaaaaactac atgcgatctg tctgcagtgt ggtgaccctg
    12661 ctacgagaac ccagcgattg attgatgggg aacctgcttc ctataatgac cctattattt
    12721 tagttggggc aaccgaaaat tatgaagcac gctgccgcag ctgtcacgaa gtgaaagaca
    12781 aaccgtaaag gagattataa tgaataaaga tgttgattac ggcggccagg ctgtcatcga
    12841 aggtgtgatg atgaaaggtt taaacaaact ggcgattgcc gtaaggaaac cggataattc
    12901 aatagttgta aaaaaaagga accttactcc cttaagcgat aaattcaaag tatttggctg
    12961 gccttttata agaggggttg tagccttatt tagttcatta atcttaggga tgcagtcttt
    13021 aacttttgct gccaaccagg ttacagaaga agatgaagaa ctaaccccac ttgaaatggg
    13081 gctaaccatc ttagtttcct taggattagc catcctatta tttgtggcct taccggccgg
    13141 aataattgcc ttaatccagg aatatattga atataatgtt gtgttgaatt tgattgaagg
    13201 cctgattaaa gtttccgcct ttttacttta catcgtagct atttcaagat tagatgatat
    13261 aaaaagggta ttcatgtacc acggtgctga gcataaggtt attcataact atgaatcagg
    13321 caaagagctg tcggttgaaa acgcgcgtga gtttacaact atgcacgcca gatgtgggac
    13381 taattttatc tttattgtta tcatactaag tatcttattt ttctcttttt tcggcaggcc
    13441 gccactatta taccgtattt tataccactt gatgctgctg cccgtcatag ccggaacttc
    13501 ttatgaagtt atcaaactgg ccgggggaga agaagttaac cctgtcatca aagttttagc
    13561 tacaccgggc ctatggctgc agaagcttac taccagtgaa cccgaagatg acatgttaga
    13621 ggtggctatt accgccctta agggggtcct gcccgaggaa gaaagaggtg aagcagaaaa
    13681 tgtttgattt agaggaaaag ttagaagaaa aatatgaaga atatcagaag ttaaataaaa
    13741 agatcagtga cccagaggta atgaatgatt ctaataaata ccagaaactt ctcaaaagac
    13801 acgctaaatt aaaggaactg atcgaatatt ataaagagta taaggaaata aaagaaggta
    13861 tcgaggaagc cgaagaaatt attgaggaag atgactctga aatggaggaa ttagcagaga
    13921 tggagcttgc ggaactaaaa cctcgtttag agaaactaga aaacaagcta cccttgatgt
    13981 tattacctga aaaccctgat gatgagaaaa acgtaattgt agagatcaga gcaggagccg
    14041 gcggtgatga agccggctta tttgcagccg acctctttag aatgtatacc cgctatgcag
    14101 aggataacag ctggaagtgg gaactgatga atgcgaatga atcaggtgtc ggcggtttta
    14161 aagaagtaat cttctcaatt gaaggtaagg aagtatataa aaagctaaaa tatgaaagtg
    14221 gagttcaccg cgtccagcgc gtgccttcca ccgaatcaag tggacggatc catacttcta
    14281 ctgcgaccgt ggctgtcttg ccggaagcag aagaagtaga tattgaaata gataccaatg
    14341 atttaaagat tgacttttac cgttccagcg gtccgggcgg ccagagtgtg aataccaccg
    14401 attcagcggt aagaatcaca catgagcctt ctggtttaac cgtttcctgt caggatgaaa
    14461 agtcacagca taaaaacaag gcgaaggcaa tgcgtgtgtt aagagcccgc ctaaaagaaa
    14521 aacgtgaaaa agaaaaacag gaagaaaggg ccgaagctag aaagagccag attggaaccg
    14581 gggaaagaag tgaaaagatt aggacctata acttcccaca gggaagagtc agtgaccata
    14641 gaattaatct gaccgaacac aaactggaac aaattttaga cggcgattta gatatcataa
    14701 ttgaaccttt aattgaagag gataccatga aaaaactaga gaaggtgtaa taattgaata
    14761 tcaaagaagt tttagtcaac acagaagaat actttcaaaa aaatggaata gaatctgccc
    14821 gacttgatgc agaagtactg ctcgctgaac ttcttgatat ggaaagaatt aaactatatg
    14881 taaactttga ctatccttta aatgatgaag agatatctca atatagagag atgattaaaa
    14941 aaagagccca gcatataccg gtagcctata taattggaca taaggaattt atgtctctgg
    15001 acttctatgt aaataagaat gtcttactgc cgagaccaga aactgaaatt ttggttgagt
    15061 atctaattga ctgctttcag gaaaaagaaa tggatactgt taatattgtg gaagtaggga
    15121 ccggtagtgg agcgattatg gtcagcctgg gcaattatct ggacaatgcc aggattttag
    15181 gtgtagaaat tgaggaagga gccttagagg ttacccgcaa aaatatcgag aggttttcac
    15241 ttgatgatag gctcaaggtt actaaaggag accttttaaa accactgatt aaaagagaga
    15301 tcaagaatgt ggacctggta gtatccaatc ctccctatat tagcgaagaa gagatggata
    15361 aactgccccc cgaagtgaaa aaagagcctg ccaaagccct ttatggggga agagccggat
    15421 tggatatcta taaagattta atcccgcagt ccagacaggt attaaaggac aatgggatgc
    15481 ttgccctaga aataggttat cagcaggctg aagctgtcaa aaatatactt gttaaaaaca
    15541 attttaaaga tattgatgtt agaaaagatt atgccgataa agatagattt attatagcat
    15601 ataaggggta agaacatgta caagacgaag gttaaaaaaa ttgcccaaaa tctcataaga
    15661 aaaaataagg tggatgtgat taaaaagagt tttgccatca aggaagcagc tgagtatatc
    15721 ttagctgatg aagtggttgc ctttcccaca gaaacggtct atggtttggg ggcaaatgct
    15781 ttaaaagaag aagctgtcag caagatattt aaggcaaagg ggcgtccttc cgataatccc
    15841 ctgattgccc acattgcttc taaagatcag ctgccggcct taatagaagg agagatccca
    15901 gccgccgctg agaaacttat tgagagattt tggcctggcc cgctcaccat tattttcaaa
    15961 aagagtaaaa aagtacctaa aataactacc gcgggtcttg atactgtagc ggtgcgaatg
    16021 ccttcccatc cggtggctct tgccctgata gaaaaggtaa atctaccctt agccgcccct
    16081 agtgctaatt catctggttt cccatcacct accaaagctg aacatgtata taatgattta
    16141 aatggaaaga tcccccttat agttgatggg ggaatttgta atataggggt agaatcaaca
    16201 gtaattgaaa tcgaaggtaa taatgtaagt attttaagac cgggtggtac ccccaaagaa
    16261 ttaattaaaa agaccctggg agataaatat caggttgaaa cacttaatca ggctgaggtt
    16321 aaaagcccta aatcaccggg catgaaatat aaacattatg cacctcatac cccactttgg
    16381 ataattcaag caggcaatat tgaaaatgtc atacaacaaa atgatgtaaa taatgaaaac
    16441 acattattaa ttctatccag tgagactttg cgggggctag aacaagattc cctccgggta
    16501 aaaatacagg atatgggttc aattgaagat ttagaacata ttgcaaaaca tctgtataac
    16561 attttgagag aaactgatga acaggatttt gaattggttt tgatcgaaga agtacctgat
    16621 aaggaaattg gtgaagctgt aatgaataga ttaaaaaaag cagctgtcaa aataatttaa
    16681 agaggagaat aattatgaaa atattattcg tctgtacagg taatacctgc cgtagtccca
    16741 tggctgaata ctttgccaaa cagtatatcc aagaaaatga tcttgaggat tttgaagtga
    16801 actcggctgg tattaatgct gtaacgggag aaaaggcttc cagtgaagca attgaagtta
    16861 tgaaagagaa ggatattgat ataacaggtc ataagagtaa gatgttgatg gaagcagatc
    16921 tcaaggagag cgacagggtt tttgcaatga ctaacagtca taaagaagta attgaagata
    16981 tgtatggata tgaggaagta tatactttaa aacactttgt gcagagttgt gaagactgcg
    17041 atattcttga cccttacggc tatgatatcg acaagtatag aaagaccagg gatgagatcg
    17101 aatatgcagt caaagagctg ttaaaaaagt taagtaatag ctaaaaaggc aggaaaaagt
    17161 taacttatct agaatactaa aagttaggat gataggtata attt
//
LOCUS       DATGCJ010000194        13924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_341738, whole genome shotgun sequence.
ACCESSION   DATGCJ010000194 DATGCJ010000000
VERSION     DATGCJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341738"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VKN64_09015"
     CDS             <1..529
                     /locus_tag="VKN64_09015"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60419.1"
                     /translation="KYLRRIDNSIKRELKNKEVPLLLYCDEGLYHYYKKVNSYNNLFD
                     KFINGNPESLNKNEIHEKSWEGVKEYIQKQKEEIIEEFQELKGTSKTSMDIKLIIPDA
                     FYSKVYSLLIDGSRKLQGYFDKQKNKVHMIKNGKKTYDLFNYAAILALRNGGDVYVFD
                     EEVPEGIDILAINRY"
     gene            complement(590..1417)
                     /locus_tag="VKN64_09020"
     CDS             complement(590..1417)
                     /locus_tag="VKN64_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMA60420.1"
                     /translation="MGKMGKTKRILLHVAIWISIFIICLPLIYAIIISTRDLQGTFVS
                     PPTLKFGTSTIETYKTAWESVNLGRLLINSGIVAITTSVVKIILAILAAFAIVFFDFK
                     GKYVAFAAVFLTQLLPVPVKIVPTFELMKTFNWVDSYYALTFPYFATATGTFLFRQFF
                     MTVPDDLVDAARIDGCSPMTFLRKVLIPLSWNNIGALFLVEFVYMWNRYLWPLIVTNS
                     EDMRVAQIGIKMLMTTGSINKWNIIMAGAIITLIPPLIVLLVLQKTFIEGFVMRTGK"
     gene            complement(1410..2294)
                     /locus_tag="VKN64_09025"
     CDS             complement(1410..2294)
                     /locus_tag="VKN64_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA60421.1"
                     /translation="MNQSYFKEKILPYLLVSPSILVIVIFLVYPFYQAVKLSFFRGGA
                     WGTNQVFVGLENYIELFSSPKYLASLGRTLLFATFVTFIGLAVSLGLAVLLNQKIRGR
                     LFYRTAIIWPYALSTVIAGVLWALLFDPSIGLITYVIEVLTGIEINWRTNGNLAFVII
                     TIAAAWRNIGYNVIFFLSALQNIPKQVLESAEIDGASPFQKFFRISIPLITPTLFFLL
                     IMNYLYAFFRTFGLVDVATQGGPGTSTEILTYKLYQDAFVFFDAGSANAQSVLLFVFI
                     VGFTYLQFKYTQRGVFYG"
     gene            complement(2368..3672)
                     /locus_tag="VKN64_09030"
     CDS             complement(2368..3672)
                     /locus_tag="VKN64_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60422.1"
                     /translation="MKKLMSVVFILALAFTFMFTINTVQAQDVEVTWWHAMSGGRLEP
                     VKHLVEEFNKTHPNITVNATFTGSYQETLNKAITAAKAGNPPNIIQTYEVGTQMMWDS
                     PYIVPIEDLAEPGEVDWGDYLSVVRRYYTNPEGKLVSMPFNSSTAILYYNKDMFEEAG
                     LDPNDPPTTYAEMTEAGRQLLDSGVVDHAITYGWPTWVFEQTHYWTDTNYVNNGNGRE
                     KRATKVLFNEEPGMKIMEKWAEWEEEGILIYGGREYEPDAAFISQNTAMLVQSTSHLK
                     DIIETSPFEVGTSFLPRPEWANRGGTTIGGASLYIFEDDQEKMDAAWEFVKWLSNTDN
                     AVYWHKQTGYFPIRYSALTKLMNEGWFSQNPNFLTAFMQVLTSKESNATSGAVVGRFP
                     QIRDIVDDAVTNIFGDEMTVEEALNSAAEEANKVIEQYNESQ"
     gene            4181..4741
                     /locus_tag="VKN64_09035"
     CDS             4181..4741
                     /locus_tag="VKN64_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMA60423.1"
                     /translation="MNPFTHVKLAKEIHYYINQNYEIDIPLKTFVWGNIKPDFKKNKK
                     LHYIDRNLKEALKMYAELNNFDYTNNKDYAVRLGEFFHYIADFFCYAHNTEFFQENLS
                     EHFIYEMRAHKKVTKFKNFFNKGIKESSIILDGSENLLDYLMYNHKKYIKNWRKPEND
                     FIYTLKLAPVLVNNIIVNNQLEAEVS"
     gene            4760..5329
                     /locus_tag="VKN64_09040"
     CDS             4760..5329
                     /locus_tag="VKN64_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HMA60424.1"
                     /translation="MIENKYKTSLSKTLSYILRHHPEDFDLEMEINGTVVINNLLEAL
                     RDDNRFANIEFEDIQQLAENDNKNRFTIIEYKNNKRIKANYGHSIEWINLDYTTIKPP
                     QFLYHGTTEKYYKNILKEGIKKMGRKYVHLSKTKQQARQVGKRRTKNPIVLKIYAEKM
                     YQDGYSFFKTNSDILLTEYVDSKYIEKTK"
     gene            5366..6316
                     /locus_tag="VKN64_09045"
     CDS             5366..6316
                     /locus_tag="VKN64_09045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMA60425.1"
                     /translation="MNRTHIFSMFLILIISVALIMSSNLILSNERIRTTNTYETELKY
                     TRVNEFYNQKHYKNDDEFIFDIKIRNYYLREHNVDPCLMEQFEFHIVQNGETLWSIAQ
                     KHGINIDTLIGANNIDNMNRIKPGDSLLILPVKGILYKIGPGQSIEGLAENFNIEVST
                     IRTSNNIGSNAEVQTGRLVLLPGAKPEFGYKDRLEKMLIAPVNARISSYYGRRWGRMH
                     EGVDYAVNIGTPIRAAGAGRVVFSGWSSGYGKAVIIEHRKGLRTLYAHNSQLLVHVGQ
                     YVERNEVISKSGNTGRSTGPHLHFEVQINSRAVNPLNYLR"
     gene            6420..8780
                     /locus_tag="VKN64_09050"
     CDS             6420..8780
                     /locus_tag="VKN64_09050"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMA60426.1"
                     /translation="MSSIYDEKLVFKIDGIGCASCAMKIEERLIQLPQVNDADLNLMS
                     NRLILILNKYNQNLESKIKEIVKEIEPNLDLSEPEFSNFKEYEYTIENISCVDCMNKI
                     ETGIKELSEVSKVNINFNTNKLRVKSKANPINDIKKIVDNIEDNAVIKEKAAVISEQQ
                     EVKGQTFLNKNKIRIFNLIFGSLLFLTAILYSNLTFLRDIGIYQETLSIFLYLATYIL
                     VGRNVLKKAVNNILNGFWFDEYFLMSIATIGAIIIGEYPEAVTVMLFYRIGELLQDKA
                     VDNSRRSIKDLMDIKPDYVNILKDNQLKKVKPNQVSVGQIILVKPGEKVPLDGVITEG
                     DSVLDTSSLTGESALKSVKKGDEIKSGVINKEGVLYIEVKSPYEDSTVAKILNLVERS
                     AEKKAKTEKFITKFAKVYTPIIVILSILVTIVPVLIFNAPFEVWLYRSLIFLVISCPC
                     ALMISIPLGYFGGIGRSSKNGILVKGSNYLEELRKVKTIVFDKSGTLTEGKLKVLNIE
                     TANDYSQEEIIELAASVESFSNHPVARAIQEYYTGELITDKITGIEEIAGKGIKAKVN
                     KQNIIIGNKKLFEKEDIPLENTDNIDNLIFIAIDGNYAGKIEVAEKIKESSFKLMQQL
                     DGIKKIMLTGDNEEKAQIVAEQLNIDKYYFSMLPDEKVYILEEILEETKEGRVAYVGD
                     GINDAPVLARADIGIAMGGLGTDAAIEASDLVIMNDNLEKIKTALDNSNLTNRIVWQN
                     IILALTVKIAVLSLGTIGLASMWSAVFADVGVALLAVLNSTRIIKK"
     gene            8794..9165
                     /locus_tag="VKN64_09055"
     CDS             8794..9165
                     /locus_tag="VKN64_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMA60427.1"
                     /translation="MEKVKNYCEVCEVFDPDTEVVKKLQDKKLDEDVIYELAETFKVL
                     GDPTRIKILYALKEQELCVCDLSELLEMSPSAISHQLRVLRNNKLVKFRKEGRSVYYS
                     LDDKHVICFLGQGLNHVLEDR"
     gene            complement(9169..10242)
                     /gene="mnmA"
                     /locus_tag="VKN64_09060"
     CDS             complement(9169..10242)
                     /gene="mnmA"
                     /locus_tag="VKN64_09060"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMA60428.1"
                     /translation="MKKVLAAMSGGVDSSVTAALLKEKDYEVIGGTMEIFPDYEQKSG
                     EEGGCCSLSSIEDAKMVAAKIGIPHYTFNLKDVFQKKVINNFVNEYSIGNTPNPCIVC
                     NKEIKFKALLNKARQIGADYIATGHYAKIKKFNGRYILKKAEDSHKDQTYMLYGFTQQ
                     QLKHTLMPLGDYSKKEIRKMAKEINFSVHDKPDSQEICFVPDNDYTRFLDDNYPEITE
                     KGPILDTAGNKLGEHNGLYNYTVGQRRGLGISAPHPLYVVKLDKENNAVIVGKDKEVN
                     FKGLTAENVNYISVPKLTQKRTALVKIRYNSPAFKAELIPVTVNKIKVIFTEIQRAVT
                     PGQSVVFYNDTAVIGGGIIKTAI"
     gene            complement(10275..10640)
                     /locus_tag="VKN64_09065"
     CDS             complement(10275..10640)
                     /locus_tag="VKN64_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HMA60429.1"
                     /translation="MYTKEVMEHFQNPRNVGELESPDTVGEARNEAHGDVIKLYIKIK
                     DNIIEEIKFKTFGCAAAVATSSMFTVLAKGENIEEALKISKQDIADALGELPDNKMEC
                     SNLAPDAFKKAIEKYRQKR"
     gene            complement(10665..11834)
                     /gene="nifS"
                     /locus_tag="VKN64_09070"
     CDS             complement(10665..11834)
                     /gene="nifS"
                     /locus_tag="VKN64_09070"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HMA60430.1"
                     /translation="MNDIYLDHAATTPMDPAVIKTVNKYMKNYYGNPSSIYQTGQKSL
                     NEISQARQSIADYINAGDLREIIFTSGGTESDNLAIKGTALARQDKGKHIITTKIEHH
                     AVLHTCEYLEKKHGFDVTYLDVDEKGFVNIDALKDAVNDNTILVSIMTANNEIGSIQP
                     IEEIGEFLKDKDIYFHTDAVQALGQLDVDVQTMNIDLLSASAHKLYGPKGVGILFIKK
                     GTKLIPQMNGGAQERNRRAGTENVPGIMGFKKAVEILKETRNEKVEKNIFLRDKIIKG
                     IENNIDNVILNGPRGDKRLSNNVNFCFKNVEGESLLLNLDMIGIAASSGSACASGSVD
                     PSHVLLAIGRDKSTARGSLRLSLGKDNTESEVEFLIEKLPGIISRIREMSPLNDN"
     gene            12170..13198
                     /locus_tag="VKN64_09075"
     CDS             12170..13198
                     /locus_tag="VKN64_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HMA60431.1"
                     /translation="MNIKKISVEKAVGKVAAHDMTQIIPDEFKGVRFKKGDIIKEEDI
                     SILKDMGKENIYVLELDDNHLHEDEAAKVMADYFISENIRLSEPHEGKISLIAEVDGI
                     LNINKDILFKANTEDDILITTGHSKSPIQKGEIIAGVRINPLVIEKSKIDRVEKILSR
                     AEIFSIASFQDYKVGVVITGNEVYEGRIEDKFLPTLKRKFRRWGGKLLDYRYVPDNTD
                     EIKEALLELKEKGAEILLTGGGMSVDPDDVTPAGIRKTKANVVKYGVPVLPGNKLMVA
                     YLEDIPTLGLPACVIFEEITVFDIVYPRILTGEKINKEKLVKLSYGGLCHHCPVCQYP
                     HCSFGKVD"
     gene            13212..>13924
                     /gene="moaA"
                     /locus_tag="VKN64_09080"
     CDS             13212..>13924
                     /gene="moaA"
                     /locus_tag="VKN64_09080"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMA60432.1"
                     /translation="MNVNYLRISVTDRCNLRCFYCMPQEGIKLKRHKDIMSYEDIYKV
                     TQVAAEIGITKVRITGGEPLVRLGVEELIEKIDSIPKIDEISLTTNGVSLYKQAEDLK
                     EKGLDRVNISLDTLDRSQFKEITGRDHLNKVLKSIDKAKELDLTPVKVNTVLIKGVND
                     NQIDEFLKFMDEKDIILRFIEYMPIGSEKDQANYVKIADIKEVLKQKYELTPAQVMGN
                     GPAKYYKVKGYKGKLGFISP"
BASE COUNT         4975 a         2063 c         2431 g         4455 t
ORIGIN      
        1 aaagtattta cgaagaattg ataatagtat caagagggaa ttgaaaaata aagaagtacc
       61 tctattatta tactgtgatg aaggactgta ccattattat aaaaaggtta attcttataa
      121 taatcttttt gataaattta ttaatggtaa tccagagagt ctcaataaaa atgagattca
      181 tgaaaaaagc tgggaaggtg taaaagaata tattcaaaaa caaaaggaag aaatcattga
      241 agaatttcaa gagttgaagg ggacttcaaa aacttcaatg gatattaaat taatcatccc
      301 ggatgcattt tatagtaaag tatattcact tttaattgat ggttctagaa agctgcaggg
      361 atattttgat aagcaaaaaa ataaagtgca tatgataaaa aatggtaaaa aaacttacga
      421 cctatttaat tatgctgcga ttttagcgtt aagaaatggc ggagatgtat atgtatttga
      481 tgaagaagta ccagaaggaa tcgatatcct ggcaattaat agatattaaa taaaataaaa
      541 aataaagtaa aaaatagcct ctaccttaat cggtagaggc tttttttaat tatttacctg
      601 ttctcatcac aaatccttct ataaaggttt tctgtaagac taacagaaca attaatggtg
      661 gaattaaggt aattatggca ccggccatta ttatattcca tttattgata gaaccggttg
      721 tcatcagcat cttaatacct atctgagcta ctcgcatatc ttcagaattg gttacgatta
      781 aaggccataa atatctattc cacatataga caaattccac taaaaataga gctccaatat
      841 tattccagga taatggaatt aaaacttttc ttaaaaaagt cataggtgaa cagccgtcaa
      901 tacgggccgc atctactaag tcatccggta cagtcatgaa aaactgccgg aataaaaagg
      961 tacctgttgc cgttgcaaaa taaggaaatg ttaaagcata gtaagaatct acccaattaa
     1021 aagttttcat caattcaaaa gtggggacaa ttttaaccgg cacaggtaga agttgggtta
     1081 aaaagaccgc ggcaaaggca acatattttc ctttaaaatc aaaaaagaca attgcaaaag
     1141 ctgccagtat agctaatatt atcttgacta cacttgttgt aatagcaact atccccgagt
     1201 taataagtaa tctacccaaa ttaactgatt cccaggcggt cttatatgtt tcaattgtac
     1261 tggtaccaaa ttttagagta gggggtgata cgaatgtacc ctggagatct ctggtactaa
     1321 taatgattgc atatataagt ggtaaacata ttatgaaaat tgatatccag attgcgacat
     1381 ggagtagtat tctcttagtc tttcccatct tacccataga agacccccct ttgcgtatat
     1441 ttaaattgta gataagtaaa tccaactatg aatacaaata gtaaaacgga ttgggcatta
     1501 gctgatccgg catcaaagaa aacaaaggca tcttggtata acttataagt taatatttca
     1561 gttgatgtac cggggcctcc ttgggttgct acatcaacta aaccaaatgt tctaaagaaa
     1621 gcatatagat agttcattat taataaaaag aacagagtag gtgtgattaa aggtatagaa
     1681 attctaaaga acttttgaaa gggacttgca ccatcaattt cagctgattc taaaacctgt
     1741 ttcggtatat tttgtagggc tgataaaaag aaaataacat tataaccgat attacgccag
     1801 gcggctgcaa ttgtaataat tacaaacgct aaattcccat tcgtcctcca gtttatttca
     1861 ataccggtta atacttcaat aacataggtt atgagtccaa tagaaggatc aaaaagtaag
     1921 gcccataaga cccctgcaat aactgttgat aaagcataag gccaaattat tgcagttcta
     1981 taaaataatc tacctcttat tttttgattt aagaggactg ctaatcctag agagaccgct
     2041 aatccaatga aggtaacaaa agttgcaaaa aggagagtgc ggcctaaact agccagatat
     2101 ttaggggatg aaaataattc tatgtagttt tctaaaccta caaaaacttg attagttccc
     2161 caggctccgc ctcgaaaaaa actaagtttt actgcctgat aaaatggata tactagaaat
     2221 attacgataa ctaatattga aggtgatact aataaatatg gtaaaatctt ttctttaaaa
     2281 tagctttgat tcataataaa aactcctttt tactctcaga taatgttaag aggtgaggtg
     2341 tttccacctc accttagttt tatttactta ttgagattca ttatattgtt cgataacctt
     2401 attagcttct tcagctgctg agttgagagc ttcttcaaca gtcatctcat caccgaatat
     2461 attggttact gcatcatcca caatatctct tatttgtggg aacctaccta ctactgcacc
     2521 tgaagtagca ttagattctt tagatgttag tacttgcatg aaagctgtta agaagttcgg
     2581 attttgtgag aaccagcctt cattcattag ttttgttaat gcactgtatc tgattgggaa
     2641 gtatccagtt tgtttatgcc agtatacagc attatcagta ttgcttaacc attttacaaa
     2701 ctcccaggct gcatccattt tttcttgatc atcttcaaag atatataagg aagctccacc
     2761 aatggttgta ccaccgcggt tagcccattc aggtcttggt aagaaggaag taccaacttc
     2821 aaatggagaa gtttcgataa tatccttcaa gtgagaggtt gattgaacta acatagcagt
     2881 attttgtgaa ataaaggcag catctggttc atattcgcgg ccaccataga tcaggatacc
     2941 ttcttcttcc cattcggccc atttttccat gatcttcata ccgggttcct cattaaacaa
     3001 tactttggta gctctttttt ctctaccatt tccattattc acatagtttg tatctgtcca
     3061 gtaatgggtt tgttcaaata cccaggtagg ccaaccatat gttatagcat ggtctacgac
     3121 acctgaatct aataactgcc tacctgcttc agtcatttct gcataagtgg taggtggatc
     3181 attaggatca aggcccgctt cttcaaacat gtctttattg tagtaaagaa tagctgttga
     3241 ggagttaaat ggcattgaaa ccaatttacc ttcggggtta gtatagtatc ttctaactac
     3301 tgatagataa tcaccccagt caacttctcc tggttcagct aagtcttcaa ttggaacaat
     3361 atagggactg tcccacatca tctgggttcc tacttcataa gtttgaatta tgttgggcgg
     3421 attaccagct ttagcagcgg taattgcttt atttaatgtt tcttgatatg aaccagtgaa
     3481 agttgcattt actgtgatat tcggatgagt tttattaaat tcttctacta agtgtttgac
     3541 aggttctaaa cgtcctccac tcatagcgtg ccaccaggta acttctacat cctgagcctg
     3601 cacagtatta atggtaaaca taaaagtaaa agctaaagcg agaataaata ctactgacat
     3661 taactttttc atttaacaat acccccttat ttttttgccg ataaagtgct aaaacagtta
     3721 tttgttttag ggtaccacct ccaataaata ttttttaatt tttttaaata ttatgagaaa
     3781 taaagttgga gtgtaaacaa cttatactat taaattgaaa aaatataaat tgtatagtag
     3841 gtagtagata gcacaaaaaa gagaagtata taataaaaaa cctttgagtt agttttatga
     3901 gaatgcaatt aagtactgct attttacact aattattata tctaaaaagg atttttaagt
     3961 caaagaaaag tatttagaaa tagatgataa tttttcctat attatcaaat ataataaaaa
     4021 gtaaagtgtt ttttgtataa taatgataat taagcaccct aaagatttgt taaattttta
     4081 atattaaata ttggattttt tgggtcatat aatgatataa taaaaaaaac tatggtataa
     4141 aaaaagaaaa atagtaaaaa agaagaggtg gaattaaaat ttgaatccct ttacacatgt
     4201 aaaattggct aaagaaatcc actattatat aaaccaaaac tatgaaatag atatccctct
     4261 aaaaactttt gtttggggca atataaaacc cgactttaaa aaaaataaaa agcttcatta
     4321 tattgatcgg aacttaaaag aagcattgaa gatgtatgct gaattaaaca atttcgacta
     4381 cactaataat aaagactatg ccgttagatt aggagaattt tttcattata tcgctgattt
     4441 cttttgttat gctcataata cagaattttt ccaagaaaat ttaagtgagc atttcattta
     4501 tgaaatgcgg gcccacaaaa aagtaactaa atttaaaaac ttttttaata agggaattaa
     4561 ggaaagttca attattttag acggtagtga aaatttacta gattatttga tgtataacca
     4621 caaaaaatat atcaaaaatt ggcggaaacc ggaaaacgac tttatataca ctttaaagtt
     4681 agcccctgtt ttagttaata atataattgt caataatcaa ttagaagctg aagtttcata
     4741 aaaatagagg tgtatatatt tgatcgaaaa caaatataaa acatctttat ctaaaacctt
     4801 atcatatatt ttacgccatc atccagaaga ttttgactta gagatggaaa ttaatggaac
     4861 agtagttatc aataatttat tagaagcttt aagagatgat aataggtttg caaatattga
     4921 gttcgaagat atacaacaac ttgcagaaaa tgataataaa aatagattta caataatcga
     4981 atacaagaat aataaaagaa taaaggctaa ctatggtcac agtatagaat ggattaattt
     5041 agattatact actattaaac ctccccaatt tctttaccac gggacaaccg aaaagtatta
     5101 taaaaatatt ttaaaagaag gtataaaaaa gatggggagg aaatacgttc acttaagtaa
     5161 aacaaagcaa caggcccgac aggtaggaaa aagacgtact aaaaatccaa tagtattaaa
     5221 gatatatgct gagaagatgt atcaggacgg ttattctttt tttaaaacta acagtgacat
     5281 tttattaacc gagtatgttg atagcaaata tattgaaaag accaaataag attttaataa
     5341 taagtatgat cataggagga taaaattgaa cagaacgcat atattctcaa tgtttttgat
     5401 actcattatt agtgtggctt taataatgag ttctaattta atattaagta atgaaagaat
     5461 tagaactact aatacatatg agacagaatt aaagtataca agggttaatg aattttacaa
     5521 tcaaaagcat tataaaaatg atgatgaatt catatttgat ataaaaataa gaaactacta
     5581 tttgagagaa cataatgtag atccctgttt gatggaacaa tttgaatttc atatagtaca
     5641 aaatggtgaa acgctttggt caattgccca aaaacatggt attaatatag atacattaat
     5701 tggtgccaat aatattgaca atatgaaccg aataaagccc ggcgacagcc ttttgattct
     5761 gccggtaaag ggtatactat ataaaatagg accgggacag agtattgaag gtctagcaga
     5821 aaacttcaat atagaagtct caacaattag aacttccaat aatataggtt caaatgcaga
     5881 agttcaaact ggccgattag tcttattacc gggagcaaaa cctgaatttg gctacaaaga
     5941 tagactcgag aaaatgttaa tcgcgcctgt taacgcccgt atatcttcct attatggtag
     6001 aagatggggt agaatgcatg aaggtgttga ttacgcagta aatattggta caccgatcag
     6061 ggcagctgga gccggacgag tagtgtttag cggttggagc agtggttatg gaaaagcagt
     6121 aattattgaa cacagaaaag gtttaagaac tttatatgca cataactcac aacttttagt
     6181 tcatgtcggt caatatgtgg aaagaaatga agtaatatca aaatccggta ataccggaag
     6241 aagtactgga ccacacttgc attttgaagt tcagattaac agtagagctg ttaatccctt
     6301 aaattattta agataaacta attaaaaaga agaataatgg tatctaaccg gtaccattat
     6361 ttatttaaca aatatatgaa caattattca tatattcata caaaaagggg gataaaataa
     6421 tgagttcaat ttatgatgaa aaattagtat ttaaaataga tgggataggt tgtgcaagct
     6481 gtgctatgaa gatcgaagaa agattaattc agcttcccca agttaacgat gctgatttaa
     6541 acttaatgtc aaataggtta atactaatcc ttaataagta taaccaaaac ctagagagta
     6601 agattaaaga gatagtaaaa gaaattgaac ctaatcttga cttatcagag cctgagtttt
     6661 ctaacttcaa agaatatgaa tatactattg aaaatataag ctgtgtcgat tgtatgaata
     6721 aaattgaaac aggtattaaa gaattatcag aagttagtaa agtaaatatt aacttcaata
     6781 ccaataaact acgagttaaa agtaaagcta atcctattaa tgatattaaa aagatagtag
     6841 ataatatcga agataatgct gttataaaag aaaaggcagc tgtaatatcc gaacaacagg
     6901 aggtaaaagg tcaaactttt cttaacaaaa ataaaataag gatctttaat ttaatatttg
     6961 gatccttact ctttttaact gcgatattat attccaacct tactttttta agagatatag
     7021 gaatatacca agaaacactt agtatcttcc tatatcttgc cacctatatt ttagttggta
     7081 gaaatgtcct taaaaaagct gttaacaata tcttaaatgg cttctggttt gatgaatatt
     7141 tcttgatgag tattgctaca attggagcaa taattattgg agaatatcca gaagcggtca
     7201 ctgtaatgtt attttacaga ataggagaac tacttcagga taaggcagtt gataattcga
     7261 gaagatctat taaagattta atggatatta aacccgatta tgtaaatatt cttaaagaca
     7321 atcagcttaa aaaagttaaa ccaaaccaag taagtgttgg ccaaataatt ttggtaaagc
     7381 caggcgagaa agttccttta gatggagtaa ttacggaagg tgattctgta ttagatacct
     7441 cttctttaac cggggaatca gctcttaaaa gtgttaaaaa gggagatgaa attaaaagtg
     7501 gtgtaataaa caaagaaggg gtattatata tagaagttaa aagtccctat gaagattcga
     7561 cagttgccaa aatattgaac ctggtagaga gatcggctga gaaaaaagct aaaactgaaa
     7621 aattcattac caagtttgct aaagtatata cacctattat agtaatatta tcaatattag
     7681 taacgattgt gcctgtttta atatttaatg ctccttttga agtatggctt tacaggtcct
     7741 tgatcttcct tgtaatttca tgcccatgtg ccttaatgat ttcaataccg ttaggttact
     7801 tcggcgggat tgggaggtca tctaaaaatg gaatattagt taaaggcagt aactacctcg
     7861 aagaattaag aaaggtcaaa acaattgtat ttgataagtc cggtacactt acggaaggta
     7921 agttaaaagt attaaatata gaaactgcca atgattatag tcaagaagaa ataattgaat
     7981 tagctgcttc cgtcgagagt ttttccaacc accctgtggc tcgggctatt caggaatatt
     8041 atactgggga acttatcacg gataagataa caggaattga agaaattgca ggtaaaggga
     8101 ttaaagcaaa agttaataaa caaaatataa taattggtaa taaaaaatta ttcgaaaaag
     8161 aagatatacc attagaaaat acagataata ttgataattt aatatttata gctatagatg
     8221 gtaactatgc aggtaagata gaagttgctg aaaagataaa ggaaagttct tttaaactaa
     8281 tgcaacaatt agatggtatc aaaaagataa tgttgacagg tgataatgaa gaaaaagcgc
     8341 aaatagttgc tgaacagtta aatattgaca agtattattt tagtatgctg cctgatgaaa
     8401 aagtatatat attagaggaa atattagaag aaacaaaaga aggaagagtt gcttatgtgg
     8461 gtgatggtat taatgatgct cctgtactgg cgcgggctga tattggaatc gcaatgggag
     8521 gactgggaac tgatgcggca atagaagcat ctgaccttgt aataatgaat gataatctag
     8581 agaaaataaa gacggcttta gataatagca acttaaccaa cagaattgtc tggcagaata
     8641 taattttagc tttaactgtc aaaatagcag tattatcttt aggtacaatc ggacttgctt
     8701 cgatgtggag tgcggtcttt gcagatgttg gtgttgcctt attagcagtg cttaattcta
     8761 caagaattat taagaaatag aggggataag attatggaaa aagtaaaaaa ttactgtgag
     8821 gtctgtgagg tatttgatcc tgatactgag gtagtaaaaa aactacaaga taaaaaatta
     8881 gatgaggatg ttatttatga actggctgaa acttttaaag tgctaggtga ccctaccaga
     8941 atcaaaatat tatatgcatt aaaagaacag gagttatgtg tatgtgatct atccgaatta
     9001 ctggagatga gtccttccgc tatttctcat caattaaggg tcttgaggaa taataaactg
     9061 gttaaattta gaaaagaagg ccgatcagtt tattattccc ttgatgataa acatgttatt
     9121 tgttttttag gccagggatt aaaccacgtt ttagaagaca gataatgttt atatcgctgt
     9181 ttttataatt cctcccccaa ttacagcagt atcattataa aatactactg attggccggg
     9241 ggttacagct ctttgtattt cagtaaaaat aacttttatc ttatttacag taactgggat
     9301 taattcagct ttaaatgctg gactattata tctaatttta acaagggctg ttcttttttg
     9361 agttaatttt gggactgaaa tatagttgac attttctgca gtaagtcctt tgaaattaac
     9421 ttctttatct ttaccaacaa ttactgcatt attttcttta tctaatttta ctacataaag
     9481 gggatgaggg gctgaaattc ccagacctct tctttgtccc acagtataat tatataggcc
     9541 gttatgttct cccaatttat tgccagctgt atctaaaata ggtccttttt cggttatttc
     9601 aggataatta tcatctaaaa accttgtata atcattatct ggcacaaaac agatttcttg
     9661 actatcgggt ttatcatgta cggaaaagtt gatttctttt gccatttttc taatttcttt
     9721 tttggaatag tctcctaagg gcattaaggt gtgttttaac tgttgttggg taaaaccgta
     9781 taacatatat gtttgatcct tatgactatc ttcagccttt ttgagtatat atcttccgtt
     9841 aaactttttt atcttggcat aatggccggt cgcaatatag tcagccccaa tttgccttgc
     9901 cttatttaaa agagccttga atttaatttc cttgttgcaa acgatacagg ggttgggagt
     9961 atttccaata ctatattcat taacaaaatt atttattact tttttttgga aaacatcttt
    10021 taaattaaag gtataatgag gtataccaat tttagctgct accatcttag catcttcgat
    10081 agaagataat gagcagcagc ctccttcctc acccgatttt tgctcataat caggaaaaat
    10141 ttccatggta cccccaatta cttcataatc tttttctttt aatagagcag cagtgactga
    10201 actatcaact ccaccgctca tagctgcaag tactttcttc attttttcca tccttcattt
    10261 ctctatatat gcatttatcg tttttgtcta tatttttcaa ttgctttttt aaatgcatca
    10321 ggagccaggt tagaacattc catcttatta tcgggtagtt ctcctaaggc atctgctata
    10381 tcctgtttac tgatctttaa agcttcctca atattttcac cttttgctaa aaccgtaaac
    10441 atactgctag tcgctaccgc agcagcacag ccaaaagttt taaatttaat ctcttctata
    10501 atattatctt taatttttat atataatttg ataacatctc cgtgagcttc attacgggct
    10561 tctcccactg tatcaggaga ttctaattct cctacatttc tggggttttg aaaatgttcc
    10621 attacttctt ttgtatacat accaatcatt actccttact ttttttaatt atcatttaga
    10681 ggggacattt ctctgattct acttataatt ccgggcagtt tttcaattaa aaattctacc
    10741 tctgattcgg tgttatcctt acctaatgaa agtcttaaag aaccacgggc tgtgctctta
    10801 tccctaccta tcgctaagag tacgtgagag ggatctaccg agccggaggc gcaggctgaa
    10861 ccactggaag ctgcaattcc aatcatatca agatttaaaa gaagtgattc accttctaca
    10921 tttttaaaac aaaagttgac attattagac agacgtttat caccacgcgg tccatttagt
    10981 attacattat caatattgtt ttctatacct ttgattattt tatctcttaa aaagatattt
    11041 ttttctactt tttcatttct ggtttctttt aagatttcaa cggctttttt aaatcccata
    11101 ataccgggta cattttcagt tccagctctt cggtttcttt cttgagctcc accattcatt
    11161 tgggggatta attttgttcc ttttttaata aaaaggattc caaccccttt aggtccatat
    11221 agtttatgag cagaagcaga gagcaggtca atattcattg tttggacatc tacatcaagc
    11281 tggcctaaag cctgtacagc atcggtatga aagtaaatat ctttatcttt tagaaactct
    11341 ccaatttctt caataggctg tattgatcct atttcattat tggcagtcat aattgagact
    11401 aagatagtat tatcatttac tgcatctttt aaagcatcta tgttgacaaa gcccttttca
    11461 tctacatcta agtaggtaac atcaaaacca tgtttttttt cgaggtattc acaggtatgt
    11521 aagacagcat ggtgttcaat tttggtagtt attatatgtt tccccttatc ttggcgagcc
    11581 agggcagtac ccttaattgc taaattatct gattctgtcc ctccacttgt aaagatgatt
    11641 tctctcagat cacctgcatt tatatagtcc gcaattgatt gacgggcttg gctgatttca
    11701 ttaagcgatt tctgtccggt ttgatatata ctagaagggt ttccataata atttttcata
    11761 tatttgttaa ccgtttttat aactgccgga tccatagggg tagtagcagc atgatccaaa
    11821 tagatatcat tcattctttt ttacctcccg tataatgaca ttaaataaaa agttgagtat
    11881 attaatcaga tttacataaa aattttaaca caccctttaa tacctgtcaa tagttatcag
    11941 tatatccaaa tatacagata aaattagatt tatataaaaa gttattaaga tattttttat
    12001 gatttgtggt aattatataa tgataaaccc atatttatat gttataatac ttatcaagat
    12061 agtcatattt atttcagtcg ctatatgagg ttgagaataa cgataaagga gccaatattt
    12121 taagaaaata aatttttaag tttcaaaaag gaacaagagg tggactttga tgaatataaa
    12181 gaaaatatca gttgaaaaag cagtgggcaa agttgcagct catgatatga ctcaaatcat
    12241 cccagatgag tttaaagggg tcagattcaa aaagggagat attataaaag aagaagatat
    12301 atcaatctta aaagatatgg gaaaagaaaa tatatatgtg ttagaactag atgataacca
    12361 tttacatgaa gatgaggcag cgaaagttat ggccgattac tttataagtg aaaatataag
    12421 attaagtgaa ccacacgaag gtaaaatatc attaattgct gaagttgatg gtatcttaaa
    12481 tataaataaa gatatactat ttaaggcaaa taccgaagat gatatcttaa ttacgaccgg
    12541 tcacagtaag agtccgatcc aaaaaggaga gataattgca ggtgtcagaa tcaaccctct
    12601 agtaattgaa aaaagcaaga tagatagggt tgagaagata ttaagccgag cagaaatatt
    12661 ttccatagca tcctttcaag attataaagt aggggtagta attactggta atgaagtata
    12721 tgaaggtaga atagaagata agtttttacc caccttaaaa agaaagttta gaagatgggg
    12781 aggcaaatta ctggactaca ggtatgtacc tgataatact gatgaaataa aagaagcttt
    12841 attagaactt aaagaaaaag gggcagaaat tttattaaca ggaggcggta tgtctgttga
    12901 tccggatgat gtaaccccag caggaattag aaaaacaaaa gcgaatgttg taaagtatgg
    12961 tgtaccggtt ttaccgggta ataaattaat ggtagcttac ctagaagata tacctacttt
    13021 aggtttaccc gcctgtgtaa tatttgaaga gataaccgtc tttgatatag tttatcccag
    13081 aattttaaca ggagagaaga ttaacaaaga gaagttagtt aaattatctt atgggggatt
    13141 atgccaccac tgtcctgttt gtcagtatcc acactgttcc tttggtaaag tagattagaa
    13201 aggacgatac tatgaatgta aattatctaa gaatctctgt aaccgataga tgtaatttaa
    13261 gatgttttta ctgtatgccc caagagggaa tcaagctaaa gagacataaa gatatcatga
    13321 gttatgaaga tatctataaa gtaacgcaag tagcagctga gataggtatt actaaagtaa
    13381 ggattacggg aggagagccc ctggtaagat taggggtaga agagctgata gaaaaaatag
    13441 attctatacc aaaaatagat gaaataagtc taacaactaa tggagtttct ttatataaac
    13501 aggctgaaga tttaaaagaa aaagggttag accgggtaaa tataagttta gacacccttg
    13561 ataggagtca gtttaaagag attacagggc gagatcattt aaacaaggtg ttaaaaagta
    13621 tagataaagc aaaagaatta gatctaactc ctgttaaagt aaatactgtt ctaattaaag
    13681 gggtcaatga taatcagata gatgaatttt taaaatttat ggatgaaaaa gatatcatat
    13741 taagatttat tgaatatatg ccgatcggta gtgagaaaga tcaagccaat tatgtaaaaa
    13801 ttgctgatat aaaagaagta ttaaagcaaa aatatgaact gacaccagct caagtaatgg
    13861 gtaatggacc tgctaaatac tataaagtaa aggggtataa aggtaaatta ggttttatct
    13921 cacc
//
LOCUS       DATGCJ010000195         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_386440, whole genome shotgun sequence.
ACCESSION   DATGCJ010000195 DATGCJ010000000
VERSION     DATGCJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386440"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VKN64_09085"
     CDS             complement(<1..380)
                     /locus_tag="VKN64_09085"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMA60433.1"
                     /translation="MKILVTGGAGFIGSNFIRYILKNKEIEIINLDKLTYAGNRENLT
                     DIEDDNNYRFIKGDICNQSLVNKIVSDNVDIIINFAAESHVDRSIEDPNKFFNTNVLG
                     TQILLDAAVKYEVDKFIQISTDEV"
     gene            549..1730
                     /locus_tag="VKN64_09090"
     CDS             549..1730
                     /locus_tag="VKN64_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60434.1"
                     /translation="MKDSSPEISFRKKYSDTIIFLLLIFLFFGYVGSQMGIGNMFSTL
                     METAYKLLMDTVFYIMAIAVLAGAFGKMATEFGLIFLLNKLFAPLMRPLYNLPGVSFL
                     GTITTYLSDNPAIISLAKEEGYLGYFKKFEVPSLCNLGTAFGMGLILTTFMAGLGFMK
                     EALIGNLGAIIGSIVSVRIMNYRVKKHMGIDRNGEEHLDKEKLENVEERNFLNRKKAT
                     EANENFMERALSAMLEGGKLGVDIGLSIIPGVVVICTIIMVLTFGAPEAGYQGGAFEG
                     VRLLPTIGSWLSPILEPLFGFSNPEAIAFPITALGAVGAALSLIPKFLSENIIGPNEI
                     AVFTAMGMCWSGFLSTHVAMMDALNRRKLISTAISSHFVGGLAAGIAANIIFNAINYI
                     I"
     gene            complement(1781..2578)
                     /locus_tag="VKN64_09095"
     CDS             complement(1781..2578)
                     /locus_tag="VKN64_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HMA60435.1"
                     /translation="MNSHVIDYEVLGDDMQVVEIELDPQETVIAEAGGMNWMDSGIEY
                     DAKMGDGTEVNDSVMNKIFGAGKRLISGESIFLTHFTNKRNIKRKVAFAAPYPGKIIA
                     LDLAEVPQNTFTCQKDAFLCAAKGTKVDITFNKRIGTGLFGGEGFILEKLSGDGKVFL
                     HAGGTVVKKELDGDMIKVDTGCLVGFTEGIDYNIQKAGKLKTMLFGGEGLFLATLYGK
                     GTVYLQSLPFSRLADRIIKSAPRLQGKRKGEGSVLGRLGDFVDGDNF"
     gene            complement(2730..3194)
                     /gene="ribE"
                     /locus_tag="VKN64_09100"
     CDS             complement(2730..3194)
                     /gene="ribE"
                     /locus_tag="VKN64_09100"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMA60436.1"
                     /translation="MAKVYEGKLVGKGQKVGIVVGRFNEFISSKLLEGALDGLKRHDV
                     EEEDIEIAWVPGSFEIPLTAKKMAQTGKYDGVICLGAVIRGETPHFDYVSNEVSKGVA
                     KVGLDTEIPVIFGVLTTDTIEQAISRAGTKSGNKGFEAAQTLVEMVNLFDNI"
     gene            complement(3227..4420)
                     /locus_tag="VKN64_09105"
     CDS             complement(3227..4420)
                     /locus_tag="VKN64_09105"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HMA60437.1"
                     /translation="MNSIEEALADIYEGKMVIVVDDEDRENEGDLIMAAEKADKESIN
                     FMIKEARGLVCTPLKEERINELDLPQMVDNNTDTHETAFTVSVDHKSTTTGISAYERA
                     LTIQKLADENSKAEDFNKPGHVFPLTARKGGVLRRAGHTEAAVDLAKLAGLKPAGVIC
                     EIIHEDGSMARLPELKEFAEKHNLKIISIEDLIKYRKKKDKLIKAEASADLPTHFGDF
                     KIKVYTTEVDDREHVAVIKGDVSGKEDVLVRVHSQCITGDIFGSLRCDCGEQLAAALQ
                     MIEEEGQGVLLYMRQEGRGIGLANKIKAYSLQDEGMDTVEANEALGFEPDLRDYGIGA
                     QILSDLGLHTLRLITNNPKKVIGLEGYGLEITERIPLEIEPNEENEFYLKIKKDKMGH
                     LLDLD"
     gene            complement(4435..>4644)
                     /locus_tag="VKN64_09110"
     CDS             complement(4435..>4644)
                     /locus_tag="VKN64_09110"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="catalyzes the formation of riboflavin from
                     6,7-dimethyl-8-(1-D-ribityl)lumazine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMA60438.1"
                     /translation="DYSPEVLKVSIIPETINSTNLKYLKAGALVNIETDIIGKYVNKF
                     LTDKKTDNSKQKELDKQMLREKGFI"
BASE COUNT         1340 a          943 c          712 g         1649 t
ORIGIN      
        1 taaacttcat cagtagaaat ttgaataaat ttatccactt catactttac agcagcatcc
       61 aataaaattt gtgttcccaa tacattggta ttaaaaaatt tattgggatc ttcaatactt
      121 ctatccacat gtgactctgc cgcaaaattt attataatat ctacattatc tgagacaatt
      181 ttgtttacta aactttggtt acagatatcg ccttttataa acctatagtt gttatcatcc
      241 tcgatatcag ttaagttctc tctgttaccc gcataggtta atttatctaa attaataatc
      301 tcaatttctt tatttttaag tatatatctt atgaagttgc tgccgataaa gccagccccg
      361 cctgtaacta aaatcttcaa cttttttcat cctttccgat atgtaataac cttatctaaa
      421 ttatatttta tcattaattt aatgaaattc aaaaatatta tttttattat tgtaatcatg
      481 attattttgc gttataatat tgaagtgttg catgtgaaaa aattattaaa attggagtgt
      541 atttttgtat gaaagacagc agtcctgaaa tttcttttag gaaaaaatat agtgatacta
      601 taatattttt attacttatc ttcttgtttt tcggttatgt aggttcccaa atgggtatcg
      661 gtaatatgtt ttcaaccttg atggaaactg cttataaatt actgatggac accgtatttt
      721 acatcatggc cattgcagta ttagcgggtg cctttggtaa aatggctacc gaattcggct
      781 taatatttct gttaaataaa ctatttgcgc cacttatgag acctttatat aacctaccgg
      841 gcgtttcttt tttaggaaca atcactacct atttatcaga taaccctgct attatatccc
      901 tggccaaaga agaaggatat ttaggttatt ttaaaaaatt tgaagtcccc tctttatgta
      961 atctcggtac tgcctttggc atgggcttaa tacttacaac ttttatggcc ggtctcggct
     1021 ttatgaaaga agctttaata ggtaatttag gggccataat tggaagtatt gtaagtgtta
     1081 gaataatgaa ttaccgagtt aaaaaacaca tggggatcga tagaaatgga gaagaacatt
     1141 tagataaaga aaagttagaa aatgttgagg aaagaaactt tttgaatcgt aaaaaagcaa
     1201 ctgaagccaa tgaaaacttt atggaaaggg ctttaagcgc catgctggaa ggtgggaaac
     1261 tgggtgttga tattggatta agtatcatac cgggagttgt agtaatctgt acaattatca
     1321 tggtattgac ctttggggcc ccggaagctg gttatcaggg aggagccttt gaaggggtaa
     1381 gactattacc gacaattgga agctggttat cacccatctt agaaccacta tttggttttt
     1441 ccaatccaga agcaattgct ttccccataa ctgccttagg agcagttggg gcggccttaa
     1501 gtttaatccc caaattcttg tccgagaata ttattggtcc caatgaaata gcggttttta
     1561 ccgcaatggg tatgtgctgg agtgggtttt taagtacaca cgttgcaatg atggatgccc
     1621 ttaatagaag gaaattaatc tcaactgcaa ttagcagtca ctttgtaggc ggcctggcag
     1681 caggtattgc cgccaatatt atttttaatg cgataaatta tataatttaa aaaggtaaac
     1741 ccccttcttt caaagggggt tttaaacatt aattctcatc ttaaaaatta tcaccatcta
     1801 caaagtcccc tagcctacct aaaacagagc cttcaccttt tcttttccct tgtaacctag
     1861 gtgcactttt tattattcta tcagccaatc gagagaaggg tagactttgt aaataaacgg
     1921 tgcctttacc gtaaagagtt gctaaaaata agccttctcc cccaaataac atggttttca
     1981 atttgccggc cttttgaata ttataatcaa tgccctccgt aaatccaact aaacaaccgg
     2041 tatcaacttt tatcatgtct ccatcgagtt cttttttgac aacagttcct cctgcatgta
     2101 aaaagacctt tccatcacct gatagttttt ctaaaataaa tccttcgccc ccgaatagtc
     2161 cggtacctat tctcttgtta aaagtaatat caactttagt acctttagca gcacataaaa
     2221 atgcatcttt ttgacaggta aaggtgtttt gaggtacttc cgctagatct aaagcaataa
     2281 tcttgcctgg ataaggggca gcaaaggcta ctttcctttt tatattcctt ttattagtaa
     2341 aatgagtcag aaaaattgat tcaccactta ttaatctttt tcctgctcca aatatcttgt
     2401 tcatgacaga atcattgact tcagttccat cccccatctt ggcatcatat tcaatcccac
     2461 tgtccatcca gttcattcca ccggcttcgg caataacggt ctcctgcgga tctaattcga
     2521 tttccaccac ctgcatatca tcgcctaaaa cttcataatc aataacatgg ctgttcactt
     2581 ttgggttcac ctctttttaa tatgtaattt ctaaacatat tataccattt tttatctaat
     2641 tctactatta attttaagtt ttattttata aaaaaagacc ctatatttaa atagggtccc
     2701 tagaataatt tattttaatt ggtattttct taaatattat caaatagatt taccatttca
     2761 accaaggttt gagctgcctc aaaaccctta ttaccggatt tagttccggc tcttgaaatt
     2821 gcctgttcta tcgtatcggt ggtaagtaca ccaaaaataa cggggatctc tgtatctaag
     2881 ccgactttag caacaccttt ggaaacttca ttggaaacat aatcgaaatg aggagtctct
     2941 cctcttatca cagctcctaa acagattact ccatcatact tgcctgtttg agccattttt
     3001 ttagctgtta agggtatttc aaaggaacca ggtacccatg ctatttctat gtcttcttcc
     3061 tctacatcat gtcgtttaag cccgtctaag gcaccttcta ataatttact ggatatgaat
     3121 tcattaaacc ttcctactac gattcctact ttttgtccct ttccaactaa ctttccttca
     3181 taaacttttg ccattataac accttcccta ttattaattt ttttaattaa tctagatcta
     3241 agaggtgacc catcttatcc tttttaatct taaggtaaaa ttcattttct tcattgggct
     3301 ctatttcaag cggaattcgc tcggtgatct ctagtccata accttctaat ccaatcactt
     3361 ttttgggatt atttgtaatt aaccttaagg tatggagtcc taaatcagat agtatctggg
     3421 ctcctatccc atagtctctt aaatccggtt caaagccgag ggcttcatta gcttccacag
     3481 tatccattcc ttcatcctgc agtgaatagg ccttaatttt attggcaagt ccaatccctc
     3541 taccttcctg tctcatataa agcaagactc cctggccttc ttcttcaatc atctggaggg
     3601 cggcggctaa ctgttcacca cagtcacatc tcaatgaacc aaagatatct cctgtaatac
     3661 attgggaatg gacccttacc aggacatctt ctttacctga aacatcacct tttataacag
     3721 ctacatgttc tctatcatct acttctgtgg tatatacttt tatcttaaaa tccccaaaat
     3781 gggtcggcaa atcagccgaa gcctctgcct tgattagctt atcctttttc tttctatatt
     3841 taattaagtc ttcaatacta attattttta aattgtgttt ttcagcaaat tcttttaatt
     3901 cgggtagtct ggccatactg ccatcttcat ggatgatctc acagatcaca ccggctggtt
     3961 ttagacctgc taatttcgcc agatcaacag ccgcttccgt gtgaccggct cttcttaaaa
     4021 ctcctccttt acgggcagtt aaaggaaata catgaccagg tttattaaaa tcttccgctt
     4081 tactattttc atcagctaat ttttggatgg ttagggccct ttcataagca gaaatcccgg
     4141 tagtagtact tttatgatct accgatactg taaaagctgt ttcatgtgta tcggtattat
     4201 tatccaccat ctggggtaaa tcaagttcat taattctttc ttctttaagg ggagtacaga
     4261 ctaaacccct ggcctcctta atcatgaaat tgatgctttc tttatctgct ttttcagctg
     4321 ccattattaa gtcaccctcg ttttctctat cttcatcatc aactactatt accattttcc
     4381 cctcatatat atcagcaagt gcttcttcta tgctgttcat aatctcaact ccctttaaat
     4441 aaatcccttt tctctcagca tttgtttatc taattctttc tgctttgaat tatcagtttt
     4501 tttatcggtt aaaaacttat ttacatattt accaatgata tccgtttcaa tattaactag
     4561 agcaccagct tttaagtatt ttaaattagt actgttgata gtttcaggta ttatcgaaac
     4621 tttaagaact tcaggtgaat aatc
//
LOCUS       DATGCJ010000196         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_205678, whole genome shotgun sequence.
ACCESSION   DATGCJ010000196 DATGCJ010000000
VERSION     DATGCJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205678"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VKN64_09115"
     CDS             <1..384
                     /locus_tag="VKN64_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMA60439.1"
                     /translation="LAVLLSSGVDLLAALTIIEGVVGNSLYAQAITEARIKVREGSTL
                     SEPLENREEFPKLLVHMIKVGEETGAMSRMLEKVSLFYDREVDRSVEGVVSLIEPLII
                     VFLAVVVGFVVISIVLPMFDMYTFI"
     gene            513..899
                     /locus_tag="VKN64_09120"
     CDS             513..899
                     /locus_tag="VKN64_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMA60440.1"
                     /translation="MSKFRKSLVENKGFTLIELLVVIAILGILAAIAVPRLAGFTDRA
                     REANVEAGGETISSNVELYIAENGKPPVLSVLTTSSSDYYIKTDEGYDIAAPTTTSAE
                     DYEYTLTGDGYVATITPTGVSVAATS"
     gene            1067..1780
                     /locus_tag="VKN64_09125"
     CDS             1067..1780
                     /locus_tag="VKN64_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HMA60441.1"
                     /translation="MIGSFLNVIIYRLPLKESIIFPASHCPMCKAKLKYYDLIPFLSY
                     LFLKGRCRYCNKKISIQYPLVELLTGLLFLLVFLKFGFTTEFIIFMVLISSLLVVSFI
                     DIKHQIIPNEITFFLIPFGLILSLFFNHISFLNSLLGLIVPAALLLLIALIYKKGMGL
                     GDVKLVAMIGVFIGWKYGLLSIFIGALIGSIYGVYMMVNTGMDRKTQIPFGPFISLGA
                     VVMILYGNLLINWYISLFI"
     gene            1802..2203
                     /locus_tag="VKN64_09130"
     CDS             1802..2203
                     /locus_tag="VKN64_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMA60442.1"
                     /translation="MVKNSNAGFTLIEVILAIAILAIALVPIFGYMINSQGVIRHADR
                     REKALILAQQELERIKNMDYDNIDNTITIDIDNTNSDLDKYPDYTGTFEPPVHYYNAE
                     GESIMKTIIITLSWGDSNEHSVTLKTKVAKR"
     gene            2200..>2455
                     /locus_tag="VKN64_09135"
     CDS             2200..>2455
                     /locus_tag="VKN64_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMA60443.1"
                     /translation="MMGLIEGKSGFTLIELLVVVVILGLVITAVLNINLGGFKFLLLN
                     RDRIKLQDEARLISTNLERQIRKSSGIDNDKSDRYNLYLEN"
BASE COUNT          799 a          286 c          492 g          878 t
ORIGIN      
        1 ttggctgtac tgctttcaag tggagtagat ttattagctg ctttaactat tattgaaggg
       61 gtggtgggta acagcttata tgctcaggca ataactgaag caaggattaa ggttagggaa
      121 ggttctactt tatctgaacc cctggaaaac agagaagagt ttcctaaatt attagttcat
      181 atgattaagg ttggtgaaga aacaggagca atgtccagaa tgttggagaa ggtttctttg
      241 ttttatgaca gggaagttga tagaagtgta gaaggggttg tatccctgat tgaaccccta
      301 attatagtat ttttagcagt tgttgttggt tttgtagtta tttcaatcgt gctacctatg
      361 tttgatatgt atacttttat ttagcaatat caaaagcata agtattattt taagggaggt
      421 gtaagtttaa gaataaggta gggtttatta taatttataa taattttaaa gggattaaaa
      481 aaataacaaa attagatagg agtgaagtta atatgagtaa atttagaaaa agtttggttg
      541 aaaataaggg ttttacattg attgaattac ttgttgtgat tgctatcttg ggtattttgg
      601 ctgctattgc tgtgcccagg ttggctggtt ttacagatcg ggccagagaa gcgaatgtag
      661 aggcaggtgg agaaactatt agtagtaatg tagaattata tattgctgaa aacggtaaac
      721 ctcctgtttt atcagtgctt acaacttcat caagtgatta ttatataaag accgatgaag
      781 gatatgacat agcagcacca acgactacaa gtgctgaaga ctacgaatat acattgacag
      841 gtgatggtta tgtagcaaca ataactccta ctggagtttc tgtagctgct acatcataaa
      901 cttaaagaaa taaattaata gggtgggccc tttcttgatc atttcaaata ggggctcact
      961 caaattgata aataagttgg tttttgttta aacaaaatta tgaatgaatt tactttagaa
     1021 aggggattaa ttcactatca aatttataat atttctttta ggattaatta ttggcagttt
     1081 tttaaatgta attatatata gattgccttt aaaagaatca ataatttttc ctgcttccca
     1141 ctgtcctatg tgtaaggcta aattaaaata ttatgattta ataccttttt taagttatct
     1201 ctttttaaaa ggaagatgta gatattgtaa taaaaagatt tctatacagt atcctctggt
     1261 tgaattgttg acaggcttat tatttttact tgtatttcta aaatttggtt ttactaccga
     1321 atttataata tttatggtat taatatcttc tttgcttgtt gtatcattta ttgacataaa
     1381 gcatcaaatt ataccgaatg aaattacttt tttcttgata ccttttggac ttattctttc
     1441 attatttttt aatcatattt catttttaaa ttcattgtta ggattaattg tacctgcagc
     1501 attattgctt ttaattgcac ttatttataa aaaagggatg gggctgggtg atgtcaaact
     1561 ggtcgcgatg attggggttt ttatcggatg gaagtatgga cttttatcga tctttattgg
     1621 tgccttaatc ggttcaattt acggtgttta tatgatggtt aatactggaa tggacaggaa
     1681 gactcagata ccatttggac catttataag tttaggtgca gtggtgatga tattatatgg
     1741 gaatttatta attaactggt atatatcact atttatataa ttgggatgga ggtaatataa
     1801 gatggttaaa aatagtaatg ctggttttac cttaatagaa gtcatcttag caattgctat
     1861 tttagctata gctttagtcc ctatatttgg ctatatgatc aattctcagg gcgttataag
     1921 gcatgctgat cgcagagaaa aggcgctgat tttagctcaa caggaattgg aaagaattaa
     1981 aaacatggat tatgataata tagataacac tattactatt gatatagata atacaaactc
     2041 tgatttagac aaatatccgg actacacagg gacatttgag ccgcctgttc actactataa
     2101 tgctgaaggt gaaagtatta tgaaaacgat aattataacc ttatcttggg gagattcaaa
     2161 tgagcactct gtcactttaa aaaccaaggt ggcgaaaaga tgatgggttt aatagagggg
     2221 aaaagtggtt ttactttaat tgaattatta gtggtagtgg tgattctcgg attggtcatt
     2281 acggctgtat taaatataaa tttaggtgga ttcaaatttc ttttgcttaa tagagatcgg
     2341 attaaattac aggatgaagc cagattaata agtacaaatt tagaaagaca aataagaaaa
     2401 tcgtcaggta tagataatga taagagcgat cgatataatc tttatcttga aaatg
//
LOCUS       DATGCJ010000197         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_205801, whole genome shotgun sequence.
ACCESSION   DATGCJ010000197 DATGCJ010000000
VERSION     DATGCJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205801"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1420
                     /locus_tag="VKN64_09140"
     CDS             158..1420
                     /locus_tag="VKN64_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMA60444.1"
                     /translation="MTNYTRFVFELKRKVSIFSKKISKNLSKPKSKFITQMIYGLLKG
                     QSVLLSNIARSLKEDILLKKTVERLSRNLENFDQQEELMTDYLNKVKPNINDNTVFCC
                     DKSDVVKPYSKKLEALDRVRDGSKGETEKGYDTFEIAALTDNKRLPIELYSHIYSSLE
                     PGFKSRNVEAKKGLNFVEKHFGKKGIYALDRGYDANLYYEYFSDGNKDFVIRSKGNRN
                     VIYRSKNLNIKKVANLYKGKYVYRYTNQDNKKRTLKFSYAPIKLPALKNKNLTLVIIR
                     GLGNKPMLLISNLKPKSKKLSLAILKAYIKRWKIEEYFRFKKQKFDFENFRVRSLKRI
                     RNLDLILSITIGFLALFSNQKKRTKLKIIVQKVSERIYDLPDFDYYAIADGFKAILDK
                     SYTGITTFLKSYYQKRRSQNNLQLSLPL"
     gene            1559..2272
                     /locus_tag="VKN64_09145"
     CDS             1559..2272
                     /locus_tag="VKN64_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMA60445.1"
                     /translation="MELIIGICDDCKEQIELVENYLLEFRKSFKINIISSTEPFKFFH
                     KLDEYRPDLVFLDIDMDELDGIKLGERIKKEYSNTIIIYITAYEKYALEAFRVRAFHY
                     LLKPITAEKFSQVFREAISLLKKDYTDNRYYTVKKKGEYISLNYEKIYYFEKVRHRVK
                     LCTLEGDISFYGTFKKLLHEIDMDFFIRCHQGYIVNKNKIRAYRDQMLFLDEKQKKIP
                     VSRSHIKEVKEMMENRLFD"
     gene            2285..2455
                     /locus_tag="VKN64_09150"
     CDS             2285..2455
                     /locus_tag="VKN64_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60446.1"
                     /translation="MKDSGNKLIGDPVSFTFEHRMLNFILILAMFMSFLGAVLDLMMC
                     NCIEVVDKNIIK"
     gene            2542..2865
                     /gene="ssb"
                     /locus_tag="VKN64_09155"
     CDS             2542..2865
                     /gene="ssb"
                     /locus_tag="VKN64_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMA60447.1"
                     /translation="MINKIILTGRLVRDPELKKTNDGVSYSKFTVAVDRNYRNSEGEV
                     DTDFIDIISWRKQAERCVEYLSKGRLIGIEGSLQVSKNESEDRIYINSIVNADRIEYL
                     DSPNN"
BASE COUNT         1144 a          354 c          518 g          935 t
ORIGIN      
        1 aaagtcagag ttattaacag acaaaattgt ggataaaaac agctgaaaca ccttcaaaat
       61 aaattatcca cagattacgc aaaaaaggtg aaacaagctc tcttttttgg tataattagt
      121 ttggagacaa aaaacaccaa aaaggagttg ttccaccatg actaattata ccagatttgt
      181 ctttgaatta aaacgaaaag ttagtatttt ctctaaaaaa atatctaaaa atctatcaaa
      241 accaaaaagt aaattcatta ctcaaatgat ttatggtttg ctaaaaggac aatcagttct
      301 tctcagtaat atagcacgtt ctttgaaaga agatattctt ttaaaaaaga ctgttgagcg
      361 tctttccaga aaccttgaaa attttgatca acaggaagaa ctaatgactg attacttaaa
      421 taaagttaag cctaatatta atgataatac tgttttttgc tgtgataaat cagatgtagt
      481 taagccctat agcaaaaaat tagaagccct tgatagagtt agagatggta gtaaaggtga
      541 aactgaaaaa ggttatgaca cttttgaaat agctgcttta actgataata aaagactacc
      601 catagagctt tattctcata tttattccag tttagaacca gggtttaaaa gtaggaatgt
      661 tgaagcaaaa aaaggactta attttgttga aaaacatttt ggtaaaaaag gcatctatgc
      721 tcttgataga ggctatgatg ctaatctata ctatgagtat ttttctgatg gtaacaaaga
      781 ttttgtgatt agatctaaag gcaatcgtaa tgttatctat aggagtaaaa acttaaatat
      841 taaaaaagta gctaacttat acaaaggtaa gtatgtctac cgttatacca atcaagataa
      901 caaaaagaga actttaaaat ttagctatgc tccaattaaa ttacccgcat taaaaaataa
      961 aaatttaact ttagtaatta ttagaggctt gggtaataaa ccgatgttat tgattagtaa
     1021 tttgaaaccg aaatctaaga aattatctct agctatttta aaagcatata taaaaaggtg
     1081 gaaaatagaa gaatatttca gattcaaaaa acagaaattt gattttgaaa attttagagt
     1141 tagatctcta aaaagaatta gaaatttaga tttaatttta tcaattacaa taggcttttt
     1201 agcactgttt tctaatcaga aaaagagaac taaacttaaa ataattgtac agaaagtttc
     1261 agaaagaata tatgatcttc cagactttga ttattatgca atagcggatg gatttaaagc
     1321 tattttagat aaatcttaca ctggaatcac tacattttta aagagttatt atcaaaaacg
     1381 acgatcacaa aataatttac agctctcttt gccattatga cagtaatgtt agtttttgta
     1441 acttttaaaa atggggaaac ttattcttat gatacaattg ataaattttt acagatacag
     1501 tataataaac ttggggttat aaatatttca tcgtttttat catcaggtgg tgaagataat
     1561 ggaattaatt atagggatat gtgatgattg taaagaacaa attgaattag ttgaaaatta
     1621 tttgctggag ttcaggaaga gttttaagat aaatattata agttcaactg aaccatttaa
     1681 gttttttcac aagttagatg aatacaggcc ggatctcgtt tttctggata ttgatatgga
     1741 tgaattagat ggtataaaac tgggggagag aattaaaaaa gaatatagta acacaataat
     1801 tatatacata acagcctatg aaaaatatgc tctggaagct tttcgggtgc gggcttttca
     1861 ttatctgcta aaaccaatta cagcagaaaa attttcgcag gtcttcagag aggcaatttc
     1921 tttattaaaa aaggattata ctgataatag atattatact gtgaaaaaga agggtgaata
     1981 tattagcctc aattatgaga aaatttatta ttttgaaaaa gtacgccacc gggtaaaatt
     2041 atgcacttta gaaggggata tttcatttta cggaactttt aaaaaattgt tacatgaaat
     2101 tgatatggat ttttttatcc gctgtcacca gggatatatt gtaaacaaaa ataaaataag
     2161 ggcttatcgg gatcagatgt tatttctgga tgaaaagcag aaaaagattc ctgtcagcag
     2221 gtctcatatt aaagaagtaa aagagatgat ggagaataga ttgtttgact gaggagagtt
     2281 aattatgaaa gatagtggga ataagttaat cggtgacccc gtcagtttta catttgaaca
     2341 ccggatgcta aattttattc tcatcctggc catgtttatg tcctttctgg gagcagttct
     2401 tgatttaatg atgtgcaact gcatagaggt agttgacaaa aatattataa aataacaaaa
     2461 attaacaaaa aaggttgaaa aaaccagccg aatctgctat aataatccta aataccaaaa
     2521 atttccaata aggaggcaga tatgataaat aaaattatct tgacaggaag gctggtcaga
     2581 gatccagaat taaagaagac taatgatggt gtgagttatt caaaatttac tgtagcggta
     2641 gacaggaact atcgtaactc agaaggtgaa gtagataccg attttatcga tataattagc
     2701 tggagaaaac aggcagaaag atgtgttgaa tatttatcaa aggggaggtt gataggtatt
     2761 gaagggtctt tgcaggtaag caaaaacgaa agtgaagatc gaatatatat aaattctatt
     2821 gtaaatgcag atagaataga atatcttgat tctccaaata attaatattt gcatgaaatc
     2881 agcagtgatt ttttaactgc tgatttttac atttgctatg ttaaaaattc acttagcata
     2941 aatgccaggg t
//
LOCUS       DATGCJ010000198         6653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_162422, whole genome shotgun sequence.
ACCESSION   DATGCJ010000198 DATGCJ010000000
VERSION     DATGCJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162422"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VKN64_09160"
     CDS             <1..730
                     /locus_tag="VKN64_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HMA60448.1"
                     /translation="SLTPTDLLHINGNYKQWDVDAANLGIRIMADRYETDIDTFMNDV
                     FKEIYYQIGSVITEVLISEEGQSISLKHSDSKYFLKKMINHINKKEEKVKFSLQCNLP
                     MIAVGAPVEAYFPQIAERMNTKLILPDYAEVANAVGTVGGKVIEKIVVMVKPGDGGGF
                     LVHSPTERKFFKDFEDAQSYGQEVGQEIAYERAASSGASNIRTVVEKNDKYSDYLGQG
                     EDTKDEDKLFIESRIEVTAVGKPW"
     gene            800..1594
                     /locus_tag="VKN64_09165"
     CDS             800..1594
                     /locus_tag="VKN64_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HMA60449.1"
                     /translation="MIIIGELINSTRDVIKEKIEDRDAEYIQDIAQQQVDAGADYVDV
                     NSGAFVWDEIEHLIWLVETVQEVVDTPLSLDSPNEEAIKKAAEIHDGIPMINSITAEE
                     ERYKKVLPLIQEYDAKVVALCLSDEGMPETADQRLKIADKLMNDLEKDGVPLDNVFID
                     PIIKPISVDGKFGYQVLDTISGITEWDTDVHITCGLSNISFGLPNRSLLNQAFLMMAM
                     DRGLDSGIIDPLDEHMMKLIKAAEVLLNRDDYGQRYLKACRSGEMD"
     gene            1613..2635
                     /locus_tag="VKN64_09170"
     CDS             1613..2635
                     /locus_tag="VKN64_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA60450.1"
                     /translation="MKKIAIIGGGIAGSQTAVELANRGNKVIIIEKSDQIGGNIKDFG
                     CKAVDICLKDNLCLVDEIFNEVKLLDNIDILFQTRVNDFTGEPGSYSLGIEREGEFSS
                     LDGLDYIVVATGYTRWSDLETGTPEVYQDDRIVWASELEEFFKGRKDHQEADQLDMPI
                     KPDSAVFIQCNGSRSIQEKARYCSRVCCGYSYRMARVLKHFFPEIDITIFFMDLQEGG
                     YLQELSYKKLEENDIDYINCKPIKVNNEEEQINIFYEDQTKGKKDNLKTDLLVLSEGI
                     HAKKVNEDWSRLFNLQLDKYGFLYPIEEEAKTHIYLAGTIKGPKDIAGTLSDSKNVAY
                     RIINDN"
     gene            2651..4000
                     /locus_tag="VKN64_09175"
     CDS             2651..4000
                     /locus_tag="VKN64_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HMA60451.1"
                     /translation="MYDVIFIGEKIKSPELKSLCIDPESLVECRGEIGSFQLKYKKEE
                     ELNNEESKYIIINLKAKRDNPIEETYGFDYRLEDLAEEEDIIFIQDYDEISPAVINRQ
                     GLKKAIAAKENYKNSEVYYLFRSMRFIDDNDSLYEEARKMGIIFLKYEMENIKIKEDN
                     TINYKRGDLDLDLTGQILTAPLLKPDEKIKRVARLLNIEMSDKDYIQSENIYLQPTLT
                     GKRGVYSLAGARGPNAYSDLDLEIEYTLNEIKSNLNDFEPLTERKREVDDQKCILCYT
                     CYRVCPHGAVEKDEELDAMKINELACYGCDACISHCPADAISYVEEESKETNDLNLKV
                     LMCENSADTAFEKLDKSQFKDLDIEKIPCASSIKKDEIFTYLRKKDSRLLVLGCFEES
                     CKHLTGDGRGERIVNEVKETLSDLELGENRVRFERLSPRMEEDLNDFLLRWKEGVNL"
     gene            3997..4671
                     /locus_tag="VKN64_09180"
     CDS             3997..4671
                     /locus_tag="VKN64_09180"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HMA60452.1"
                     /translation="MIVAEQKNVKEIIDMLGDDFENLLIAGCGTCVTVSLAGGEREVK
                     SLAEILRTYYANQEREVNIETTTVKRQCDFEFIDEMEEAVKRNDIVLSLACGVGVQHV
                     VEKFPEKVVLPGLDTSFYGATTAVGEWSERCIGCGDCVLGDFFGICPIARCSKHLLNG
                     PCGGSQNGECEVDPSIDCAWQLIYDRAEALGKLDELIDYLEPKNWETYRDGGPRTLVN
                     EEYHED"
     gene            4685..5608
                     /locus_tag="VKN64_09185"
     CDS             4685..5608
                     /locus_tag="VKN64_09185"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HMA60453.1"
                     /translation="MAKNYSKVKTMLDAGHFVVCSEMGPPIGADPEFIREKANYLKGY
                     VDGVNVTDNQTAIVRMSSIAAAKIAMEEGLEMIIQVTARDRNRIGLQSDILGASALGL
                     RNVLLLTGDHQSFGRDPGAKNVFELGSTQLIMAVKDMVDNGVLLNGDEMDVPPKMFIG
                     GAANPFADPFEMQMIKMQKKVEAGVEFFQTQAIYNMEKFEYWMEEVRKRGIHEKAYIL
                     AGILPTRSPKALRMMKKYVAGMDVPDHVIKRMDQADDKEKEGVKMAVETIQKVREIEG
                     VRGVHLMPVAWEQITPEVIEEASLYPRPEVE"
     gene            5609..6052
                     /locus_tag="VKN64_09190"
     CDS             5609..6052
                     /locus_tag="VKN64_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMA60454.1"
                     /translation="MDNNFKNEITSRPGGENLMMCYSCGSCTATCPISEIDESFNPRM
                     LIKKSLLGYKEEVLNNDDLWKCIQCRRCVAHCPQNVKFADIMRVLRNMSIEAGYYPAS
                     LKDKVDKLDKAVYQYRLDIVADHLDETKDMEKSIEVMASGGDRDG"
     gene            6045..>6653
                     /locus_tag="VKN64_09195"
     CDS             6045..>6653
                     /locus_tag="VKN64_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA60455.1"
                     /translation="MAKPVLVLGGGIAGIQAATDLAEMGIPVYLVEKKPALGGKMAQL
                     DKTFPTNDCSTCILAPKISDCYNHENVTTYTYSELENLRGTQGNFTATIKQKPRYVDV
                     DLCTGCGECVEKCPVTVEDDFNQGIDSRSAIFKYQDQAVPNVVTIDAENCLKLTKGVC
                     GLCEKTCDKGAINYDQKPETIDLDVASVVFAAGYDAFSGNIVS"
BASE COUNT         2281 a         1062 c         1550 g         1760 t
ORIGIN      
        1 ctctttaacc ccgaccgatc tcctccatat taatgggaac tataagcagt gggatgttga
       61 tgccgctaat ttaggcatta ggattatggc agatagatat gaaaccgata tagatacttt
      121 tatgaatgat gtatttaaag aaatatatta tcaaatcggt tccgtaatta cagaagtcct
      181 aatttcagaa gaaggtcagt caatctcact aaaacacagt gacagtaaat actttttaaa
      241 gaaaatgatc aatcatatca ataaaaaaga ggaaaaggtt aagtttagtt tacagtgcaa
      301 cttaccgatg atcgcggtag gtgcacccgt agaagcctac ttccctcaga ttgccgaaag
      361 gatgaatact aaattgatct taccggacta tgccgaggta gcaaatgcag ttggtacagt
      421 aggtggaaag gtcatcgaaa agatcgtggt gatggtcaaa ccgggtgatg gcggcggatt
      481 tttagtccac agtccgaccg aaagaaagtt tttcaaagat tttgaagatg cccagtccta
      541 tggtcaagaa gttggtcagg aaatcgctta tgaaagggcg gccagttcag gcgcttccaa
      601 tattagaact gttgtggaaa aaaatgataa gtacagcgac tacttaggac agggtgaaga
      661 tactaaagat gaagacaaat tatttataga gagtagaata gaagtaaccg cggttggaaa
      721 accgtggtaa taattaaaac taactttgtt agggaggtgg aatgattatc gaattaaaaa
      781 gttcgataat caagatctta tgataattat tggtgaattg attaactcta cacgtgatgt
      841 tattaaagaa aaaattgaag acagagatgc agaatatata caagatattg cccaacaaca
      901 ggttgatgca ggtgctgatt atgtagatgt aaactcaggt gcttttgtct gggatgaaat
      961 agaacattta atctggctgg tggaaactgt acaggaagta gtagatacac ccttatcttt
     1021 agatagtccc aatgaagaag ctattaaaaa agcagctgaa attcacgatg gtattccgat
     1081 gattaattcc attacagccg aagaagaaag gtacaaaaag gttcttcctt taatacagga
     1141 atatgacgct aaagtagtag ccctttgttt aagtgatgaa ggtatgccgg aaaccgctga
     1201 ccagagatta aagatagcag ataaactgat gaatgatcta gaaaaagatg gggttccctt
     1261 agataatgtc tttattgatc ccattattaa gccgatcagt gttgacggta aattcggtta
     1321 tcaggtatta gatacaatca gcggtataac tgaatgggat accgatgttc atattacctg
     1381 tggtttaagt aatatttcct ttggactacc caatagaagt ctacttaacc aggccttctt
     1441 gatgatggcc atggatcgag ggttagattc aggtattatt gatccacttg atgaacatat
     1501 gatgaaactt attaaagcgg ctgaagttct cttaaataga gatgactatg gacaaagata
     1561 cttaaaagcc tgtagatctg gtgaaatgga ttaaccaaaa ggagcgagtg taatgaaaaa
     1621 gatagctata atcggaggtg gaatcgcagg atctcaaaca gcagttgaac tggctaatag
     1681 aggtaataaa gtaataataa ttgaaaaatc cgatcaaata ggcggtaata tcaaggattt
     1741 cggctgtaag gccgttgata tctgccttaa ggataacctc tgccttgtag atgaaatctt
     1801 taatgaagta aagcttttag ataatataga tatacttttt cagaccagag taaatgactt
     1861 tacaggggag cccggcagct acagtttagg aatcgaaagg gaaggggaat tcagctcgct
     1921 tgatgggctg gactatattg tcgtagctac gggctacacc cgctggtctg acttagaaac
     1981 cgggaccccg gaagtttatc aggatgatag aattgtctgg gcctctgagt tagaagagtt
     2041 ctttaaaggc aggaaagacc atcaagaagc tgaccaatta gatatgccga tcaagccgga
     2101 ttcagctgta tttatacagt gcaatggttc aagaagtata caggaaaaag ctcgatattg
     2161 ttcacgcgtc tgctgtgggt attcttaccg gatggcccgg gtattaaaac acttctttcc
     2221 tgaaatagat attacgatct tctttatgga cttacaggaa ggtggatact tacaggagtt
     2281 aagttacaag aaattagaag aaaatgatat tgactatatt aactgtaaac caatcaaagt
     2341 taataatgaa gaagaacaga ttaatatctt ttatgaagac cagactaaag gtaaaaaaga
     2401 caatttaaag actgatttat tggttttatc agaaggtata catgctaaaa aagtaaatga
     2461 agactggtcc cgcctattta atctccagct cgataaatac ggcttccttt atccaattga
     2521 ggaagaagcc aaaacccata tttatctggc cggtactatc aagggaccaa aagatattgc
     2581 tggcacctta agtgacagca agaatgtagc ttatagaata ataaatgata attagacagg
     2641 gggatatatg atgtatgacg taatatttat cggtgaaaaa atcaaatcac ccgaacttaa
     2701 gtccctgtgt attgatcctg aatccctagt agaatgtcgg ggagagatcg gtagctttca
     2761 attaaaatat aaaaaagaag aagagctaaa taatgaggaa agtaaatata taataattaa
     2821 tctcaaagca aaaagagaca atcccattga agaaacttat ggttttgatt acaggttaga
     2881 ggatcttgca gaagaagagg acataatatt tatccaggac tatgatgaga tctctcccgc
     2941 tgtaattaac cgacagggat taaagaaagc aattgctgcc aaagaaaatt ataagaacag
     3001 tgaagtatac tatctcttcc gctcaatgcg ttttatcgat gataatgaca gcctttatga
     3061 agaggcccgt aaaatgggga tcatcttctt aaagtatgag atggaaaata ttaagataaa
     3121 agaagataat acaatcaact ataagagagg cgatcttgat ttagacctaa caggtcaaat
     3181 cctaaccgcc cctttattaa aaccggatga aaagataaaa agggtggcca ggctattaaa
     3241 tatcgaaatg agtgacaaag attacatcca atctgaaaat atctatcttc agccgaccct
     3301 gaccggtaaa cgcggtgtat acagtttagc cggagccaga gggcccaatg cttactcaga
     3361 tttagattta gagattgaat atacgcttaa tgaaatcaaa tctaatctga atgattttga
     3421 acctcttacc gaacggaaaa gagaggttga tgatcaaaaa tgcatcctct gctatacctg
     3481 ttatcgggta tgtccccatg gggcagttga aaaggatgaa gaattagatg cgatgaagat
     3541 caatgaactg gcctgttacg gttgtgacgc ctgtatttca cactgtccgg ctgatgctat
     3601 ctcatatgta gaggaagaat caaaagaaac taatgatcta aatctaaaag ttctaatgtg
     3661 tgaaaactcg gccgatacgg cctttgaaaa attagataag tctcaattta aggatttaga
     3721 tattgaaaag attccctgtg ccagcagtat taaaaaagat gaaattttca cctatctaag
     3781 aaaaaaagac agtagattac ttgtcttagg ctgttttgaa gagtcctgta aacacctcac
     3841 cggtgatgga cgcggggaac gtattgtcaa tgaagtaaaa gaaactctaa gcgacctaga
     3901 attaggagaa aacagggtaa ggtttgaacg tctttcaccg agaatggaag aagatctaaa
     3961 cgatttcctg ctgcggtgga aagagggtgt taacctatga ttgttgcaga acaaaaaaat
     4021 gtaaaagaaa taatagatat gttaggtgat gacttcgaaa acctcctaat tgccggctgt
     4081 ggaacctgtg taacggtcag tctggccggt ggggaaagag aagttaaatc actggcagaa
     4141 attttgagaa catactatgc caatcaggag agagaagtaa atattgaaac cactactgtt
     4201 aaacgacagt gtgacttcga atttatagat gaaatggaag aagccgtaaa aagaaatgat
     4261 attgttttat ctctggcctg tggtgtaggt gtacagcatg tagtagaaaa attcccggaa
     4321 aaagtagtac taccaggtct agacacctct ttttacggtg ctacaactgc tgtcggtgaa
     4381 tggtcggaaa gatgtatagg ctgtggggac tgtgtattag gtgacttttt cggaatatgc
     4441 ccgatagccc gctgttcaaa acatctttta aacggtccct gtggaggttc acaaaatggt
     4501 gaatgtgagg ttgacccctc gatagattgt gcctggcagt tgatctatga ccgagctgaa
     4561 gcactgggca aactggatga attaatagat tacttagaac cgaagaattg ggagacatac
     4621 cgcgacggcg gtcccagaac attagtgaac gaagaatatc acgaggactg aaaggagtaa
     4681 taaaatggct aaaaactatt ctaaagttaa aactatgtta gatgccggtc attttgtggt
     4741 ctgcagtgaa atgggacctc ctatcggggc agatccggag tttatcagag aaaaagccaa
     4801 ctacttaaaa ggttatgtag atggtgttaa tgttactgat aaccagacag caattgtcag
     4861 gatgtcttct attgcagctg ccaagattgc aatggaagag ggccttgaga tgatcatcca
     4921 ggtcacagcc cgggatagaa acaggatagg cttacaaagt gatatcctgg gagcaagtgc
     4981 cctgggactg aggaatgttt tactgcttac cggtgatcat caatcctttg gccgggatcc
     5041 aggtgctaaa aatgtatttg aattgggatc aacacaatta atcatggcag taaaggacat
     5101 ggtagataat ggagtactat tgaatggaga tgagatggat gttccgccta agatgttcat
     5161 tggtggagcg gcaaatccgt tcgctgatcc ttttgaaatg cagatgataa agatgcagaa
     5221 gaaagtagaa gcaggggtgg aattttttca aacacaggct atctataata tggagaagtt
     5281 tgagtactgg atggaagaag ttagaaaacg cggtattcat gaaaaagcat atatcttggc
     5341 cggtatctta ccgaccagat ctccgaaagc gttaagaatg atgaaaaagt acgtagcagg
     5401 aatggatgta cctgaccatg tgataaagag aatggatcag gcagacgaca aggaaaaaga
     5461 aggagttaaa atggctgtag aaactatcca aaaagttaga gaaatagaag gggtaagagg
     5521 agtacacttg atgccggttg cctgggaaca gataacacca gaagtaatag aagaggcttc
     5581 tctttatcca agaccggagg tggaatagat ggataataac tttaaaaatg aaataacctc
     5641 ccgcccgggt ggagaaaact taatgatgtg ttacagctgt ggcagctgta cagcaacctg
     5701 tccgatcagt gaaatagatg aatcatttaa tcccagaatg ctaattaaga aatcactttt
     5761 gggctataaa gaggaagtat taaataatga cgatctatgg aaatgtatcc agtgcaggcg
     5821 ttgtgtagca cactgtccac agaatgtgaa atttgcagat ataatgagag tactgagaaa
     5881 tatgtcaata gaagccggtt attatccggc cagtttaaaa gataaagttg ataaattgga
     5941 taaagctgtt tatcaataca gactggatat tgtagctgat catttggatg aaacaaaaga
     6001 catggaaaaa agtatagaag taatggctag tggtggtgat cgtgatggct aagccggtac
     6061 tcgttctggg aggtggtata gcaggaatcc aggctgctac agacctggca gaaatgggta
     6121 tcccggttta tctggttgag aaaaaaccgg ctttaggtgg aaagatggca cagttggata
     6181 aaacatttcc aactaatgac tgttctacct gtatattagc ccctaagatt agtgactgct
     6241 ataaccatga aaatgtaacc acctatacct atagtgaact ggaaaattta agaggaactc
     6301 aaggtaactt taccgctacc attaaacaaa aacccagata tgtagatgta gatctctgta
     6361 ccggttgtgg cgaatgtgta gaaaaatgtc ccgttacggt tgaagatgac tttaaccagg
     6421 gtattgacag ccgcagtgcc atctttaagt atcaggatca ggctgtgccc aatgtggtta
     6481 caattgatgc cgagaactgt ttaaagttaa cgaaaggggt ctgtggactc tgtgaaaaaa
     6541 cctgtgacaa gggagctata aattatgatc aaaagccaga gaccattgat ctcgatgtag
     6601 catcggttgt ctttgcggct ggttatgatg ctttttccgg caatatagtg agt
//
LOCUS       DATGCJ010000199         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_431063, whole genome shotgun sequence.
ACCESSION   DATGCJ010000199 DATGCJ010000000
VERSION     DATGCJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431063"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VKN64_09200"
     CDS             <1..714
                     /locus_tag="VKN64_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60456.1"
                     /translation="GLIISISAFLYTLSLFMFFGIEEKASIVKEKESIKNLLKNVFTY
                     FKNNKNFSKSMAVLGFSMLSSIALPFYIVYAKTTFNILDSTVGIFIIVQTLGKILGGL
                     IYGIVGDRHGHHRSMLIYSISTTTAPIIAIITGMFFADYIIILYSLLYFLLGFFMNGW
                     PIFFNYMIDSVAKEDRTLYAGLVNVVKIPASLAPFAGGIIVSFWGYYPMFYLALFFSL
                     IGLFFAINLKDARDKKELL"
     gene            828..2078
                     /locus_tag="VKN64_09205"
     CDS             828..2078
                     /locus_tag="VKN64_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HMA60457.1"
                     /translation="MDIKKDLSLTIIVLLLGIPAAALTYLFIFIIDNISINIINYFSG
                     SVLLIFIPALGGLIVGLLLKYGTLTAKGHGVPLLLSTIKSEQKGLTKSDLRYEGLATT
                     FTVITGGSVGLVGPIIELTTGITDIIGRYLKFDLKNYQILIGSGAAASFAAVFCAPFA
                     GILFSLEVIHKDWSLKNIFFTAMAAFSGNYFIGFIKPKYGYFFQNINIQQSTLDVNQY
                     IYITIFSFFMAFIGWLFINTLLLDEHIFSNFEMPLYLKPMFGGLLVGMIGYFFIEIMG
                     TSSNLITNLNKLDYTILMITALLLLKIAATGFSIGSGGSGGLFAPMILTGLFSGLLAG
                     ELGLLILPSFAVSPVFLMLCGVVGIFGGIIKAPITSVILVLELFYLPNLIIPLIIAGF
                     VPFIILNLLKVRSIYSPELFNKEL"
     gene            2153..2314
                     /locus_tag="VKN64_09210"
     CDS             2153..2314
                     /locus_tag="VKN64_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HMA60458.1"
                     /translation="MQKYQCTVCMYIYDPEEGDETQGIEPGTPFEDLPDDWVCPDCGV
                     GKEMFEPYE"
     gene            2462..>3695
                     /locus_tag="VKN64_09215"
     CDS             2462..>3695
                     /locus_tag="VKN64_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HMA60459.1"
                     /translation="MSTAQILVSGYFVVIITGTLLLMLPAATTKAGPMSFINALFTAT
                     SATCVTGLIVVNTASAFTVFGQLVIMVLIQIGGLGIMTMSTLVAFIIGKKITLKERLI
                     IQEDLNQFKISGLVRLIRYVLGFTFLFEGIGALIMFIRLLGDYDVVGAFYMAVFHAVS
                     AFNNAGFDLFGNSLEGFTGDVTINFVVMILIIIGGIGFGVITEISNFNKFKHLSLQSK
                     IVILISVFLIIFGMVVIFGFEYTNPETMGNLSFGDKMLSSLFLSVTPRTAGFNTVPTG
                     SLRNSTLFFIIVLMFIGASPGSTGGGIKTTTFGVMAVTLWNLMIGKKDMEIFNRQLEK
                     EIIFKALAITMISAGLIILVTLTLSITEQGDFLDILFETVSAFGTVGLSTGVTPTLSK
                     IGRALIIFTMFAGRVGPLT"
BASE COUNT         1085 a          586 c          676 g         1348 t
ORIGIN      
        1 ggtcttatta tctctattag tgcatttctt tatacattgt cattatttat gttttttggt
       61 attgaagaga aggccagtat tgtaaaagaa aaagagagta tcaaaaattt attaaaaaat
      121 gtatttacat actttaaaaa caataagaac ttttcaaagt cgatggcagt attgggcttc
      181 agtatgttaa gttctattgc cttaccattt tatatcgttt atgccaagac aacttttaat
      241 attttagaca gtacagttgg tatctttatt attgtccaga ccctcggaaa gatattagga
      301 gggcttatct atggtatagt tggcgatagg cacggtcacc ataggtcaat gttaatctat
      361 tccataagta ccacaactgc tcctatcata gctattataa ctggtatgtt ctttgcagac
      421 tatataataa tactatattc cttactatat tttctcttgg gctttttcat gaacggctgg
      481 ccgatatttt ttaactatat gatcgattca gttgccaaag aggatagaac cttgtatgcc
      541 ggtttagtta atgtagttaa aatacccgct tctttagcac cgttcgccgg tggtattata
      601 gtcagttttt ggggctatta tcccatgttt tacctggctt tattcttttc attgattgga
      661 ttattctttg caatcaattt aaaagatgcc agagataaaa aagaactcct gtaaacagga
      721 ttattcttta atatattgaa tataaataag tagtaaatca tgcttcaact tcaacagttg
      781 gagttttatt tttattaaat ttaattgtaa tgggactgat gaacaatatg gatataaaaa
      841 aggatctatc actaacaata attgtattgc ttttaggaat acccgccgca gctttaactt
      901 atctatttat ctttatcata gataatattt ctattaatat cattaattat ttttccggta
      961 gtgtcttgct aattttcata cccgccctcg ggggactaat tgttggctta ttattaaaat
     1021 atggtacttt aaccgccaaa ggacatggag taccattact cttatcaaca attaaatctg
     1081 aacaaaaagg tcttactaaa agtgatctta gatatgaggg attagcaact acatttacag
     1141 ttattacagg tggttcagtc ggtttagtag gtcctattat tgaactaact accgggatta
     1201 ctgatatcat cgggcgttat ttaaagtttg atttaaaaaa ttaccagatt ttaataggta
     1261 gtggggctgc cgccagtttt gcggctgttt tttgtgcccc ttttgccggt atactttttt
     1321 ccctggaagt tattcataag gactggtccc taaaaaatat cttttttaca gctatggcag
     1381 ctttttcagg taattatttt ataggcttta ttaagcctaa atatggttat ttctttcaga
     1441 atattaatat acagcagagc actttagatg taaatcaata tatttatata actatctttt
     1501 cattttttat ggcctttatc ggctggctat ttataaatac gcttttatta gatgaacata
     1561 ttttttctaa ttttgagatg cccctgtatc ttaaacccat gtttggtgga cttttagtag
     1621 gaatgattgg atattttttc atagagatca tggggaccag tagtaatctg attactaatt
     1681 taaataagtt ggattacact atcctgatga tcactgcttt attactgctt aaaatagcgg
     1741 ctaccggttt ttcgattggt tcagggggct cgggcggcct atttgctccc atgatcctga
     1801 ccggcctatt ttcaggctta ttggctggtg aattaggttt attaatacta ccatcctttg
     1861 cagtaagtcc tgtattttta atgctctgtg gagtggtagg aatctttggg ggaattataa
     1921 aagcacctat tacaagtgtg atattagtat tagagttatt ttatctacca aatttaatta
     1981 tacctcttat aattgcaggc tttgtgccct ttataatact taatttatta aaggtgagaa
     2041 gtatttattc accggaacta tttaataagg aattgtaaaa tattcacaat tattgacatg
     2101 cctgcttttt atggtataat tgaattaata aaataaggaa ggggagatgt taatgcaaaa
     2161 atatcaatgt actgtatgta tgtatatcta tgatccagaa gagggagatg aaacccaagg
     2221 tatagagcct ggtactcctt tcgaagacct acctgatgac tgggtctgcc cagactgtgg
     2281 agtgggaaaa gaaatgtttg aaccttacga ataaacacat aattaacaaa ataagagtaa
     2341 ataaaccggg ttttcccggt ttttactttt attttaaaat attatataat gtattttgag
     2401 atgaactttt aaggggttgt tgaccttgtt taaaaaatta gcgaatttta acatagaaga
     2461 tatgtctaca gctcaaatat tggtttccgg ttattttgta gtaatcataa caggtacctt
     2521 actattaatg ttaccagccg ctaccactaa agccggcccg atgagcttta ttaatgcctt
     2581 atttaccgct acttccgcta cctgtgtaac cggtctaatc gttgttaata ctgcctcagc
     2641 ttttacggtc tttgggcagc ttgtaataat ggttttaatt cagatcggtg gactgggtat
     2701 catgaccatg tcaaccctgg tagcctttat tatcggcaaa aaaatcacct taaaagaaag
     2761 attaattatt caagaagatt taaaccagtt taagatttca ggcttagtgc gcttgatcag
     2821 atatgtatta ggattcactt tcttatttga aggaataggt gccttaatta tgtttatccg
     2881 gctcctcggt gattatgatg ttgtcggtgc attttacatg gcagtattcc atgcggtctc
     2941 tgcctttaac aatgctggtt ttgacctctt tggtaattct ttagaaggtt ttactggtga
     3001 tgtgacaatc aactttgtag ttatgatttt gattatcatt ggagggatcg gttttggtgt
     3061 tatcacagag atctccaact tcaataaatt taaacattta agtttacagt ccaaaattgt
     3121 aattttaatt tcagttttcc ttattatatt cggtatggta gtcatctttg gctttgaata
     3181 tactaacccc gaaacaatgg gtaatttaag ttttggggat aaaatgcttt catccctatt
     3241 tctatcggtt acacctagaa ctgccggttt taatacagta ccaaccggtt ctttaaggaa
     3301 ttctacttta tttttcatca tagtattgat gtttatcggg gcttcacccg gttcaaccgg
     3361 tggtggaatt aagactacca cctttggggt aatggcagtt acattatgga acttaatgat
     3421 cggaaaaaaa gatatggaaa tatttaatag acagctggaa aaagaaataa tatttaaagc
     3481 ccttgcgatt actatgattt cagcaggttt gataatctta gtcactttaa ccctgtcaat
     3541 aacagagcag ggagactttt tagatatatt atttgaaacc gtttctgcct tcggtacagt
     3601 tggtctatcg accggagtta cccctacctt aagtaagata gggagagctt taataatctt
     3661 tactatgttt gccggtaggg taggaccatt aacct
//
LOCUS       DATGCJ010000200         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_73315, whole genome shotgun sequence.
ACCESSION   DATGCJ010000200 DATGCJ010000000
VERSION     DATGCJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73315"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..188)
                     /locus_tag="VKN64_09220"
     tRNA            complement(113..188)
                     /locus_tag="VKN64_09220"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Asn,seq:gtt)
     gene            complement(283..2577)
                     /locus_tag="VKN64_09225"
     CDS             complement(283..2577)
                     /locus_tag="VKN64_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HMA60460.1"
                     /translation="MKKYYRGLIYLILILLICSLSITAAPPGMKDVTFKYEPQIDEVV
                     SVYLAGSFNNWATKKIEMTDENGDGVYEITISLKPGTYEYKLVVNGSMWKKDPHSEDY
                     APDGFGGLNSVINVGQQIKKVGKVGDGEIIKSALWHEQEIPAHITRLGNNIIEIRFQT
                     AVNDVEDVRLNFFDEDGYQVEYMNYFTSDEQFDYWTKRIEVKDNEFRYRFVVDDAETR
                     FWFGREGLANTMADWFRYNKENYPLFKVPAWVKEAVFYQIFPERFYNGNPDNDPEYIE
                     TYKNKTERYENIGPGWYQGVKESEHHYIDPVKFMNKSREINPKSGWHVLYGGDFQGIM
                     EKIDYLKELGINAVYFNPVFEATSNHKYNTIGYEFIEDNFAVKGNQAASNKLFIQLIE
                     NFHAEGIKVVLDGVFNHTGYEHYAFQDVIENGKDSKYWDWYYINSYPIVTLYEQRTAN
                     KQPNYESWAGFGSLPKLNVNNEEVKEYIYEITKKWMDPNGDGNPADGIDGWRLDVANE
                     VKDRNPEFWQNWRDYMKEINPDAYITGEIWGDAGEYLKGNEFDGVMNYRFRDAVVNLL
                     IDNWGVDKFINEINKVKAEYPEPAYNSLLNLIDSHDTERFLNTVGNNKKKLKTAAFLQ
                     FVMPGAPMVYYGDEVGIEGASDPDNRRTMLWEESANPYQLDKGLLRFYKQLIEIRKEN
                     EVLIEGSLNISSAGDNGSILLINRELAGEKIVGLINTSSKKREIKFQLNKEEVVDLIS
                     QNSYSLKDGELSVMVDSHGLYLFK"
BASE COUNT          818 a          587 c          363 g          965 t
ORIGIN      
        1 aatatcatca ctattgataa tacttttttg aaatgcataa atttaattca ctctcctcat
       61 gagtttaatt aataaatatt atttagtttt ttattgcatg cggtcaaaaa aatggctccc
      121 cgagcaggac tcgaacctgc ggctcgatgg ttaacagcca tctgctctac caactgagct
      181 atcggggaat attgttcctg ctttcatttt ttacattata acaataatag agggctttgt
      241 ccagttattt gattttaaaa aaatttaaat ataaatctgg ttttatttaa atagatatag
      301 gccatgacta tcgaccataa ctgacagttc gccatctttt agactataag aattttgact
      361 tatgagatca acaacttcct ctttatttaa ctgaaattta atttcccttt tcttactgct
      421 tgtattaatt aatccaacta ttttttctcc cgctagttct ctattaatta ataatatact
      481 accgttatct ccggctgaac taatatttaa actgccctct attagcactt cattctcttt
      541 tcttatctct ataagctgct tataaaatct caagagtcct ttatcaagtt gatagggatt
      601 tgcagactct tcccataaca tggtccttct attgtccgga tcacttgcac cttcaatacc
      661 tacttcatca ccgtaataga ccatcggggc ccccggcatg acaaactgta aaaacgcggc
      721 tgtttttaat ttctttttgt tatttcccac cgtatttaaa aatctttcgg tatcatggct
      781 gtcaattaaa tttaagagag agttataggc cggttcagga tactcagcct taactttatt
      841 tatctcattg ataaacttat ctactcccca attatcgatc aatagattaa cgactgcatc
      901 tctaaaacgg tagttcatca caccatcaaa ttcattacct ttgagatatt cgcctgcatc
      961 cccccaaatt tcgcctgtga tatatgcatc cggattgatc tctttcatat aatcacgcca
     1021 gttctgccaa aattcgggat ttctatcctt aacttcattg gcaacatcca acctccagcc
     1081 gtcaataccg tcggccgggt tgccatcccc attagggtcc atccactttt ttgttatttc
     1141 ataaatatat tcttttactt cttcattatt aacatttaac ttcggtaatg aaccaaaacc
     1201 agcccaggac tcatagttgg gctgcttatt ggcagtccgc tgttcatata aagttactat
     1261 cggatagctg tttatataat accagtccca gtacttagaa tctttaccat tttcaattac
     1321 atcctgaaag gcataatgtt catagccggt atggttaaaa acaccatcaa gtacaacttt
     1381 tattccctca gcatgaaagt tttcaatcaa ttggataaat aatttattag aggctgcctg
     1441 attgcctttt acagcaaaat tatcttcgat aaattcatag cctatagtat tatatttatg
     1501 gtttgaagta gcttcaaaga ccgggttaaa gtaaactgca ttaataccta attctttaag
     1561 ataatctatt ttttccatta taccctgaaa atctccccca taaagaacat gccagcctga
     1621 tttaggattg atttctcgac tcttattcat aaatttaaca gggtcaatat aatgatgctc
     1681 actttctttt accccctggt accaacccgg tccaatattc tcgtaacgtt cagtcttgtt
     1741 cttataggtc tctatatatt caggatcatt atcgggatta ccgttataaa atctctccgg
     1801 aaatatttga taaaagactg cttctttaac ccaggcaggg actttaaaga gaggatagtt
     1861 ctccttatta tacctaaacc aatcagccat cgtgtttgca aggccctctc ggccaaacca
     1921 aaatctagtt tcagcgtcat caactacaaa gcggtatcta aattcattat cttttacttc
     1981 tatccttttt gtccagtaat caaactgttc atcggaagta aagtaattca tatactcaac
     2041 ctgatatcca tcttcatcaa aaaaattaag tctaacatct tctacatcat taacagccgt
     2101 ttgaaatctt atctcgataa tattattacc aagtctggta atatgggccg gtatctcctg
     2161 ttcatgccat aaagcacttt ttattatctc tccatctcca actttcccga ctttttttat
     2221 ctgctgtcca acattaatta cagaatttaa acccccaaaa ccatcgggag catagtcttc
     2281 actatgagga tcctttttcc acattgaacc gttaactaca agcttgtatt cataagtacc
     2341 tggttttaaa gagatagtta tttcatagac tccatctccg ttttcatctg tcatttcaat
     2401 ttttttagta gcccagttat taaaagaacc ggccagataa acagaaacta cctcatctat
     2461 ctgtggttca tatttaaagg taacatcttt catccccggt ggggcggctg taattgatag
     2521 actacatatc aataagatta aaatgaggta tattaaacct ctataatatt ttttcattaa
     2581 aattttcctc ccaaaaatta atccgctaat atatatttaa ataaagggtt ttcatatcct
     2641 ttaattaaaa tatagaatta aaatttttac caaattaaag gtataagtct gtactttacc
     2701 ctttcacaat agtctatata tccctctagt tct
//
LOCUS       DATGCJ010000201         5853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_29285, whole genome shotgun sequence.
ACCESSION   DATGCJ010000201 DATGCJ010000000
VERSION     DATGCJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29285"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VKN64_09230"
     CDS             complement(<1..229)
                     /locus_tag="VKN64_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60461.1"
                     /translation="MRNIKNYKFEIIIFLTIIALSIYSAPARTFNINDNISINKTELK
                     QGDILVVKTAKEININQIKFNGNQYQTKKYEQ"
     gene            335..1357
                     /locus_tag="VKN64_09235"
     CDS             335..1357
                     /locus_tag="VKN64_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60462.1"
                     /translation="MNNLSVIGSVIKKDMKEFLRNKTIIIVIILPLLASLFFLIIEDA
                     GLNKQFNIGIVEESNNLSIYVHENIKNINVIEYKSVERASKDIGEKVAGVVHVKGQKE
                     YEIYLNAGNTTSYFFLQNQLENIIERYLNIPLQFQLKVNPVNRIEGKLGFLPIWLTIT
                     VTMIGVLIISGNLAEEKENKTMDAVYITPASNLLFLTGKILSGVLLSTITALIMLFIN
                     GFYRQPLKNIFTVFLAIFLGSLVFNLIGLIIGVKSESQSAARSIGTIIYFPLLFPTLI
                     YNLSDFTEILAKFFPTYYLFQIVNNLLSIDANFALIWYNLSILFVFAVVLASILIYIF
                     KKVYTQ"
     gene            1354..1836
                     /locus_tag="VKN64_09240"
     CDS             1354..1836
                     /locus_tag="VKN64_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60463.1"
                     /translation="MKKKKILIFLFIFVFIFSLPIMAEENKESPHEIVKEFILKVYDK
                     YQQNKFAEVYEFMHPDIKEVLEKENYLSFQNENTEKYNLKISEVEVLEVQKIEEWPEA
                     FQEIIKEKEKESLYEIKMKYKTNYQSGGNENEKMIEKKTYVVRYQEKTYLLWDPNIIK
                     "
     gene            1857..2771
                     /locus_tag="VKN64_09245"
     CDS             1857..2771
                     /locus_tag="VKN64_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60464.1"
                     /translation="MELNDYPIVIDGLKKEYKKVKALKGISFKVKKGEVFGYLGPNGA
                     GKSTTLNILTGLLKPTTGESFIFGERSNNLSKSTKQKIGVVFETNNLYQRLSGRENLN
                     FFADLYNVQSSKVEELLDFFDLSAAGDRLVKNYSKGMRQRLLIARALLTAPELLILDE
                     PTSGLDPQSVEMIHQAIEEFAGAGKTVLISTHFMEEADRLCDRIAFINEGEIVTITPP
                     SELKSRYGTKQVEVVIKKEEEKLDKLTQLVDIEYIKKEKERILIELPLISEDLGSKID
                     KIKSEFEVLSIHTREATLHQVFLELIKK"
     gene            2784..3758
                     /locus_tag="VKN64_09250"
     CDS             2784..3758
                     /locus_tag="VKN64_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HMA60465.1"
                     /translation="MDFAKPRVIISKCLGFAHCYYSGQMFNIDFLNKLGEYVEYFPVC
                     PEMEIGLGVPRQTIRIVRDKETHVLKLIQPKTEKDLTEKMENFSKDFLKEFEEVDGFI
                     FKERSPSCGHKDIKVYSGIKGQVIHHNGVGFFAQKAFDLFPLTAVESDGRLNNLRLRE
                     HFLTKLFALANFRKISTEGKIKHLINYHANNKYLLMAYDQEKLREMGRIVANHADRPV
                     KDVYDLYEEKLNNILDEPPHYKSIINVLQHIFGYFKNELTPGEKQFFLNIVDKYRDNK
                     VPLSVPVHLLRSWIVEYDINYLKDQSFINPFPEELIDLTDSGKNNDRL"
     gene            3776..4768
                     /locus_tag="VKN64_09255"
     CDS             3776..4768
                     /locus_tag="VKN64_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HMA60466.1"
                     /translation="MFKKLKTIYKPELYHGHNKGKQDFFEGWYFKVADKKAENVFAFI
                     PGVFQSESKNNEHAFIQVLDGRRNESFFIKYAFEKFRAAEDRFFIEIENNKFSKEEII
                     LDIQAKEIKIKGKLNFYNLKPWPVDILSPGAMGWYGFIPFMECNHGVLSFTHQITGDL
                     KANSEAIDFDKGKGYLEKDWGSSFPSAWLWLQSNHFNEKSSSLMVSIATIPWLSREFR
                     GFIIGFLHKDHLYKFTTYNNANINKFQKNEDIIEINVENKGYKLFIKAYYKEGTLLKG
                     PFRTDMVENVEESIQAIVEIKLVEKATNKIITEDKGKKAGFELNGNLEEIIDNF"
     gene            4835..5545
                     /locus_tag="VKN64_09260"
     CDS             4835..5545
                     /locus_tag="VKN64_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA60467.1"
                     /translation="MSEEKVLIVDDEKEIRELIRDYLEVEGFQVKMAEDGEQALNFFD
                     SFKPDIIVLDIMLPKVDGMEICKIIRGKSNIPILMLSAKKSDVDKVLGLGLGADDYMT
                     KPFSPKELVARIKAQLRRYNQLSKSNFKNNSLEFKDLKIDLDGYNVYLEGEKVQFSAK
                     EFEILKFLALNPERVYSREQIFDQIWGFNEFGDINTVTVHIRKIREKIEEDSSNPQYI
                     ETVWGVGYKFVGDQDGMV"
     gene            5532..>5853
                     /locus_tag="VKN64_09265"
     CDS             5532..>5853
                     /locus_tag="VKN64_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60468.1"
                     /translation="MAWFKNLFKMNLRKKLIIYFILIVVIPLIFYFVFFNILGAYLNN
                     SASIEEVDTVIEEFENGIRDNINLLKNPQEFKSSIDSLLTEYQGELQIIDPETDMIVL
                     DTKGS"
BASE COUNT         2106 a          749 c         1088 g         1910 t
ORIGIN      
        1 cttgttcata ttttttagtt tgatattgat ttccgttaaa ttttatttgg ttaatattta
       61 tttctttagc tgtttttact actaaaatat ctccctgttt tagttctgtt ttattaatgg
      121 atatgttatc attaatatta aaggtccggg caggagccga ataaatagag agagctatta
      181 tagttaaaaa tattattatt tcgaatttat aattttttat atttctcata gctaacatcc
      241 tttattatat ttattatctt actataatat attttactaa taattactat ttcctttaat
      301 tttaaaataa ttatttgatt cgtaggtgaa tgtaatgaat aatttatcag tgataggttc
      361 tgtaatcaaa aaagatatga aagaattttt aaggaataaa acgatcataa ttgtaataat
      421 attaccatta ttggcttcct tatttttcct tattatcgaa gatgctggat taaataagca
      481 gttcaatatt ggtatagtgg aagaaagtaa taacttaagc atatatgtac atgaaaatat
      541 taaaaatata aatgtaattg aatataagag tgtagaaaga gccagtaaag atattggtga
      601 aaaggtcgcg ggagtagttc atgttaaagg acaaaaagag tatgaaattt atctcaatgc
      661 cggtaataca accagttact tttttttaca aaatcagttg gaaaatatta ttgagcgtta
      721 tcttaatata cctttacagt ttcaattaaa ggttaatccc gttaacagga tagaaggaaa
      781 attaggcttt ttaccgatct ggcttactat cactgttact atgattgggg ttttaattat
      841 ttctggtaat ctggccgaag aaaaagagaa taaaacgatg gatgcggtct acattacccc
      901 agcttcaaat cttttatttt taaccggcaa gatattatcc ggtgttttac tttcgaccat
      961 tacggccttg attatgttat ttattaacgg tttttacaga cagccgctaa aaaatatttt
     1021 tacggtcttt ctagctatat ttttaggttc cctggtcttt aatttaattg gtcttatcat
     1081 aggcgtaaaa tcggagtcac agtcagctgc ccgttctatt ggtacaataa tatatttccc
     1141 cttattgttc ccgactttaa tatataattt atctgatttt acagaaattt tagcgaagtt
     1201 tttccctact tactatttat tccaaattgt caataactta ttaagtatag atgcaaactt
     1261 tgcacttatc tggtataatt tatctattct attcgtattt gctgtagttt tagctagtat
     1321 cttaatttat atatttaaga aggtgtatac tcaatgaaga agaaaaaaat attaatattt
     1381 ttatttatat ttgttttcat attttcacta ccaattatgg ccgaagaaaa caaagaaagc
     1441 cctcatgaaa ttgttaaaga atttatcctt aaggtctatg ataaatacca gcagaataag
     1501 tttgcagaag tatatgaatt catgcatcct gatattaaag aagtattaga aaaggaaaac
     1561 tatctttcat ttcaaaatga aaatactgaa aaatataatt taaagatatc agaagtggaa
     1621 gttttggaag tacaaaaaat agaagaatgg cctgaggcct ttcaagaaat tattaaagag
     1681 aaagaaaaag agagccttta tgaaataaaa atgaagtata aaacaaatta tcagtccggt
     1741 ggtaatgaaa atgaaaagat gattgaaaag aaaacatacg tagtaagata tcaagaaaaa
     1801 acttatcttc tatgggatcc caatattatc aagtaaattc gaagcaggtg ttatcgatgg
     1861 aattaaacga ttatccaatt gtaattgatg gtttaaaaaa agaatataaa aaagtgaaag
     1921 ctttaaaagg tatttctttc aaagtaaaaa aaggagaagt atttggatat ctaggtccaa
     1981 atggggccgg taaaagtacg accttaaaca ttttaaccgg gctcttgaaa cccacaaccg
     2041 gtgaaagctt catctttggt gaaaggtcta ataatttaag taaaagcact aaacaaaaga
     2101 tcggggttgt atttgaaact aataatcttt atcaaaggct atccggccgc gaaaatttaa
     2161 atttctttgc tgatttatat aacgttcaaa gcagtaaagt ggaagaatta ctggatttct
     2221 ttgatctaag tgcggcggga gaccgcttag ttaagaatta ttcgaaaggg atgcggcagc
     2281 gattattaat tgcaagggca ttattaaccg cccccgaact ccttatcttg gatgaaccga
     2341 ccagtggttt ggatcctcaa tcggttgaaa tgatccatca ggcgatagaa gaatttgccg
     2401 gggcaggtaa gacggtcctg atcagtactc actttatgga agaagcagac cgtttatgtg
     2461 acagaatcgc tttcataaat gaaggtgaga ttgttactat aacgcctcca tcagaattaa
     2521 aaagtcgtta tggaaccaaa caggttgagg tagtaattaa aaaagaagaa gagaaattag
     2581 ataaattaac tcaactggtt gatattgaat atataaaaaa agaaaaagaa agaatattaa
     2641 ttgaactacc tctaatcagt gaggacttag gtagtaaaat agataaaatc aaatcagaat
     2701 ttgaggtttt aagtattcat accagggaag caacccttca tcaggtattt ttagagttaa
     2761 ttaaaaagta aaggagccaa attatggatt ttgctaaacc cagggtaata ataagcaaat
     2821 gtctgggatt tgcccactgc tattacagcg gccagatgtt taatattgat tttttaaata
     2881 aattaggtga atatgtagaa tattttccag tttgtcctga aatggagata ggtttgggag
     2941 ttccccggca aacgattaga attgtcagag ataaagaaac ccatgtgtta aaacttattc
     3001 agcctaaaac tgaaaaagat ttgacagaaa aaatggaaaa cttttcaaaa gactttttaa
     3061 aagagtttga ggaagttgat ggcttcatat ttaaagagcg ctccccttct tgcggccata
     3121 aagatattaa agtctattcc gggataaaag ggcaggttat tcaccacaat ggtgtgggct
     3181 tttttgcgca gaaagctttc gacttgtttc cccttaccgc agtagaaagt gatggtcgct
     3241 taaataattt aaggttaaga gaacattttt taaccaaatt atttgccttg gctaatttta
     3301 gaaagataag tacagaaggt aaaataaaac atttaatcaa ttatcatgcc aataataaat
     3361 atcttttaat ggcctatgat caggagaaat taagagagat gggccggata gtggctaacc
     3421 atgcggatag acctgtaaaa gatgtttatg atctctatga agaaaaatta aataatatat
     3481 tagatgaacc accacattat aaatctataa taaatgtttt acagcatatc ttcgggtatt
     3541 ttaagaatga acttacccct ggcgaaaaac agtttttcct aaatatagtt gataaataca
     3601 gggacaataa ggtccccctt tcagtgccag ttcatctttt aagatcatgg attgtcgagt
     3661 atgatatcaa ttatttaaag gaccaaagct ttattaatcc gtttcccgaa gaactgatcg
     3721 atctcaccga ttccggaaaa aacaatgatc gactttaagt aaagaaggga ttattatgtt
     3781 taaaaagtta aaaaccatct ataagcctga actttatcac ggacataata aaggaaagca
     3841 agattttttt gaaggttggt attttaaggt tgctgataaa aaagcagaaa atgtttttgc
     3901 ctttatccct ggtgtttttc agagtgaatc taaaaacaat gagcatgctt ttatccaggt
     3961 attagatggc agaagaaatg aatccttctt tatcaaatat gcattcgaaa aattccgggc
     4021 tgccgaggat cggttcttta ttgaaattga gaataataag ttttcaaagg aagaaataat
     4081 attagatatt caggctaaag agattaagat taaggggaaa ttaaactttt acaatttaaa
     4141 gccctggccg gttgatattt tatctcccgg tgcaatgggc tggtatggtt ttattccctt
     4201 catggaatgt aatcatgggg ttttaagttt tacccatcaa attactggag atttaaaagc
     4261 gaatagtgaa gccatagatt ttgataaggg aaaaggttat cttgaaaagg actggggcag
     4321 cagttttcca agtgcctggc tttggctgca gtccaaccat tttaatgaaa aaagtagttc
     4381 ccttatggta tctattgcaa caattccctg gttatccagg gaatttcgtg gttttattat
     4441 aggttttctc cataaagacc atctatataa gttcaccact tacaataatg ccaatataaa
     4501 taagtttcaa aaaaatgagg atattataga aataaatgtt gaaaataaag gttataaatt
     4561 atttattaaa gcctattata aagaaggtac cttattaaag gggccatttc ggactgatat
     4621 ggttgagaat gtggaggaga gtatacaggc aattgtagag attaaattag ttgaaaaagc
     4681 tactaataaa atcataacag aagataaagg taaaaaggcc ggttttgagt taaatggtaa
     4741 tttagaagaa ataattgata atttttaaaa ggaatatttt ggaatacaac taaatatatt
     4801 aatatataat aaattttttg aatgaggtgg aaaaatgtcg gaagagaagg tcttaatcgt
     4861 ggatgatgaa aaagagatac gagaattgat tagggattat cttgaagttg aaggatttca
     4921 ggttaagatg gccgaggatg gagaacaagc cttaaatttt tttgattctt ttaaaccgga
     4981 tattatcgtg ttggatatta tgctgcccaa agttgatggt atggagatct gtaaaattat
     5041 taggggtaag tcaaatattc ctattttaat gttaagtgcc aaaaagagtg atgtagataa
     5101 agtcttagga ctgggtctag gagcggatga ttacatgact aaacctttca gtcccaaaga
     5161 gttagtagcc cgaattaagg cacagttgag aagatataat cagctgtcta agtctaactt
     5221 taagaataac agcttggagt ttaaagattt aaagatcgac ttggatggat ataatgtcta
     5281 tctggaagga gaaaaggtac agttttcagc taaagaattc gaaatattaa agtttttagc
     5341 cttaaatccg gaaagggttt atagtagaga acaaatattt gatcaaattt ggggttttaa
     5401 tgaatttggt gatataaata ctgttactgt acatataaga aagatcagag aaaagatcga
     5461 agaagattct tccaacccac agtatataga aactgtctgg ggtgtaggat ataagtttgt
     5521 tggtgaccaa gatggcatgg tttaaaaacc tgtttaagat gaatctaaga aagaaattaa
     5581 taatatattt tattttgatc gtagtaatac ctctaatatt ttattttgta ttttttaata
     5641 ttttgggagc ttatttaaat aatagtgctt ccattgaaga agtagataca gtaatagaag
     5701 agtttgaaaa cgggataagg gataatataa atttattaaa aaatccacaa gaatttaaaa
     5761 gttctataga ttctttatta actgaatatc agggtgaact tcagattatt gatcctgaaa
     5821 ctgacatgat tgttttagat actaaaggtt ctg
//
LOCUS       DATGCJ010000202         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_73764, whole genome shotgun sequence.
ACCESSION   DATGCJ010000202 DATGCJ010000000
VERSION     DATGCJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73764"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VKN64_09270"
     CDS             <1..621
                     /locus_tag="VKN64_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMA60469.1"
                     /translation="SLAKITGEYLDYIDQHIMDLEYTSEFLVIAAELIEIKAKYLLPK
                     TDEEEKEEDEESLVERLKKYQLYKELSFVLADNFEERRDVFENNSSLERYRNYEEEVV
                     IEVNLKEIKKMYLKALYSDNDLEDDDIEEVEHIIIEKIKVEKKINDVLDILDKYNKVE
                     FYKLIEDHSNIMEVVVTFLSILELMKLNKVKVLQDQLFSPIEVSLA"
     gene            635..1171
                     /gene="scpB"
                     /locus_tag="VKN64_09275"
     CDS             635..1171
                     /gene="scpB"
                     /locus_tag="VKN64_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMA60470.1"
                     /translation="MEVENMAKLETIIFVADKPVGIDKLSRVMEMKISNLRPLLDKLK
                     DEYKKESHGFELNSYDDHYVFETKKKYSDLILNYLNISKHKKLSSAALETLAIVAYEQ
                     PITRREIESIRGVNVERTLSTLEKYDLVEEKGRKETIGNPIIYGTTTIFLQKFGLDDI
                     SDLPKIEDIDYQKVFEDE"
     gene            1198..1683
                     /locus_tag="VKN64_09280"
     CDS             1198..1683
                     /locus_tag="VKN64_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMA60471.1"
                     /translation="MDKFNKRTYSSFSEFLKDIKFLIKNHGQIKDLNRGELISKKMQE
                     RLMLAVTAVNGCRYCTYYHTKLALENGVTTEELDTLLNGSVDSSPSEEKLALLYAQHW
                     ADQAGKPDQEFKEKINETYGNQKAEVINLAIRTINFGNLMGNTFDYLLYKLSFGHLGA
                     S"
     gene            1813..2526
                     /locus_tag="VKN64_09285"
     CDS             1813..2526
                     /locus_tag="VKN64_09285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMA60472.1"
                     /translation="MERLQKVMAHAGVASRRKSEEIIAEGRVKVNGVVVTEMGTKVDP
                     AQDTIEVDGEEIEKETKTYLKLHKPRGYVTTVNDPQGRQTVMDLIHGIDKRIYPVGRL
                     DLDSSGLLLLTNDGDLTHKITHPSHELDKEYMVVVNGELSQEEINRFKNGIQLEEGKT
                     SPAEIEKVNQDPKNTTYQVIIHEGMNRQIRRMFDRLGYEVVSLIRVRIGNISLGSLKP
                     GEHRKLSSKELQDLLRLLK"
     gene            2560..2826
                     /locus_tag="VKN64_09290"
     CDS             2560..2826
                     /locus_tag="VKN64_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HMA60473.1"
                     /translation="METLKVSGSSDPKSVAGAIAAVVRDEGKVELQAIGAGAINQTVK
                     AIAISRGYVAPLGIDLIMIPEFIEIDVDDEVRTAIKFTVSPRRS"
     gene            2922..>3376
                     /locus_tag="VKN64_09295"
     CDS             2922..>3376
                     /locus_tag="VKN64_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA60474.1"
                     /translation="MFLKKIKIKGFKSFANTINLKFDIPITAIVGPNGSGKSNIVDAV
                     RWVLGEQSAKNLRGSEMADVIFAGSEKLPPSSSAEVSLFLDNSERILDIETDEVKISR
                     EVTKDGSSDYLINDSKCRLKDVNELLMDTGLGNNSYSIVSQGKITQIINS"
BASE COUNT         1243 a          508 c          704 g          921 t
ORIGIN      
        1 tcgctggcca agataaccgg tgaataccta gattatattg accaacatat catggatcta
       61 gaatatacca gtgaattttt ggtcattgca gccgaactga ttgagataaa ggctaagtac
      121 ctattaccaa aaacagacga ggaagaaaaa gaagaggatg aagaaagtct tgttgaaagg
      181 ttaaaaaaat accagctgta caaagaacta tcttttgttt tagctgataa ttttgaagaa
      241 cgacgcgatg tatttgaaaa taatagttct ttggaaagat accgcaacta tgaggaagaa
      301 gtagttatcg aggttaattt aaaggaaatt aaaaagatgt atcttaaggc cctttattcc
      361 gataatgatc tagaagatga tgatatcgag gaagttgagc atataattat tgaaaaaata
      421 aaggtagaaa aaaagatcaa cgatgttctt gatattttag ataagtataa taaagtagag
      481 ttctacaaat taatagaaga ccacagcaat ataatggagg ttgtggtaac atttttaagt
      541 atattagagt taatgaaatt aaataaggta aaagtactac aggaccaact tttttccccg
      601 attgaggttt cattagcata aagcaggtga tataatggaa gtagagaata tggcaaaact
      661 ggaaacaata atatttgtgg cagacaaacc ggtcggtata gataaactat caagggtaat
      721 ggagatgaag atctctaatt taagaccatt actggataaa ctaaaagatg aatataaaaa
      781 agaaagccat ggttttgaac taaatagcta tgatgatcat tatgtatttg aaaccaaaaa
      841 gaaatacagt gacctaatct taaattactt gaatatatca aaacacaaaa agctaagttc
      901 agctgcctta gagaccttag caattgttgc ctatgaacaa ccaattacca gacgagagat
      961 cgaatcgata cggggagtta atgtagagag gaccctttct accctggaaa aatatgattt
     1021 agtagaagaa aagggccgga aagaaacgat cggtaatccg atcatttacg ggaccaccac
     1081 tatcttttta cagaagttcg gtctcgatga tatttcagat ctgcctaaaa ttgaagatat
     1141 cgattatcag aaagtatttg aagatgaata gaaattatta taaatgagga aaaaattatg
     1201 gataagttta acaagagaac ctacagcagt ttcagtgaat ttttaaagga tattaaattt
     1261 ctgatcaaaa accacggtca aattaaagac ctaaataggg gtgaattaat cagtaaaaaa
     1321 atgcaggaac gtttaatgtt ggcagttaca gccgtgaacg gctgccgcta ctgcacatat
     1381 taccatacaa aactggcctt agagaatgga gtaactactg aggaacttga taccctctta
     1441 aatggttctg ttgacagttc gccttcagaa gaaaagctgg ccctattata tgcccagcac
     1501 tgggcagacc aggccggtaa acccgaccag gagtttaaag aaaagatcaa tgaaacctat
     1561 ggtaatcaaa aggcagaagt tattaacctg gctattcgta caattaactt tggtaactta
     1621 atgggaaata cctttgatta tctcctttat aagctttctt ttggacatct gggggcttct
     1681 taataagtta ttgtataaat gtattaaaaa gaggcaaata attatataaa aaccatacaa
     1741 acttttattc tataaaaaag gaatatctta atttttaaag aataatataa tatgctaaga
     1801 aaggatgtaa atatggaacg ccttcaaaag gtaatggctc atgcaggagt cgcttcccgc
     1861 cgcaagtcag aagaaattat tgcagagggc cgtgtgaagg taaatggtgt cgtggtaact
     1921 gagatgggaa ctaaagttga cccagcccag gacactatag aagtggacgg ggaagaaatt
     1981 gaaaaagaga caaaaacata cttaaaatta cataaaccta gaggttatgt gacaactgtg
     2041 aacgaccccc aaggcagaca aacggttatg gacttaatac atggtattga caagcgaatt
     2101 tatccagttg gtagattgga cttagacagt agtgggttat tgcttttaac caatgatggt
     2161 gatctgacac ataagatcac acatccctcc cacgaattgg ataaagaata tatggtagtg
     2221 gtaaatggtg agttaagtca agaagaaata aacagattta aaaacggcat tcaattagag
     2281 gaaggtaaaa cttcaccagc tgagatcgaa aaggtcaacc aggatcccaa aaacactacc
     2341 tatcaagtaa ttatccatga aggcatgaat agacagatca ggaggatgtt tgatcgatta
     2401 ggatacgaag ttgttagttt aattagagtc agaattggca atatttcatt gggcagttta
     2461 aaaccaggcg agcatcgaaa attgagtagt aaagagctac aagatctatt aaggttatta
     2521 aaataaccta tgtagtaaaa aataaggggg agtaatcaca tggaaacttt aaaagtttca
     2581 ggttcatctg atccaaaatc tgtagcaggt gcgattgctg cagtagtacg cgatgaaggc
     2641 aaagtagagt tgcaggcaat aggagctgga gcaatcaatc agacagttaa ggctatcgca
     2701 atttctagag gttatgttgc acctttagga atcgacttaa ttatgatacc tgaatttatt
     2761 gaaatagatg tggatgatga ggttagaact gcgattaagt ttacagtttc acccagaaga
     2821 tcataaaaaa atatatactt tttaatatcg actctgggcc tatccttagt atgggatagg
     2881 tttttctctt gtaaataaag caaactttga ggtgaagaaa cttgttttta aaaaagatca
     2941 agattaaagg attcaaatcc tttgcaaata caattaattt aaaattcgat atcccaatta
     3001 cagccattgt gggaccgaat ggcagtggta aaagtaatat cgtagatgct gtccgctggg
     3061 tcctgggtga acagagcgcc aaaaatttac gcggctctga aatggcggat gtaatatttg
     3121 ccgggagtga aaaactacca ccctcttctt cagcagaggt ttccctcttt ttagataatt
     3181 ccgagcgtat cttagatatt gagaccgatg aggttaagat ttcacgcgag gtaaccaaag
     3241 atggttccag tgattactta atcaatgaca gcaaatgccg gttaaaagat gttaatgagc
     3301 tgctcatgga taccggttta ggtaataact cctattccat tgtcagccag ggtaagatta
     3361 ctcagattat taactc
//
LOCUS       DATGCJ010000203         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_29597, whole genome shotgun sequence.
ACCESSION   DATGCJ010000203 DATGCJ010000000
VERSION     DATGCJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29597"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VKN64_09300"
     CDS             <1..657
                     /locus_tag="VKN64_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMA60475.1"
                     /translation="KYSAEPEYNVFKHYLELQDEWENILNFADDNFPELQDIFKDTLV
                     GETKEKVEVKKDIKSYQNIIDLPIFKTVSEEELDELLSISQFKNYEIGDKILESGDRD
                     DALFIMQEGKVKIVNNKEEEKEIKLAELEKGDFFGLTSLIASKSANYLVDVVAQTPVK
                     LVKINRDDIFDFFSANHESSVKLLLYFMKKLNEQLEDLTEMYTDVQLKGRDISQIMDE
                     "
     gene            complement(644..2392)
                     /locus_tag="VKN64_09305"
     CDS             complement(644..2392)
                     /locus_tag="VKN64_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HMA60476.1"
                     /translation="MNKAAVYHESIDNLSYPLDDNSLRVRLVTDKNDLNKVKIIYGPK
                     FDFYKDPVYSKEMEVVGSDQKHDYYSVKIHLEDPRFRYHFLLEDYQGNKYWYNEKGFF
                     ENRPRGHEAGFFQYPIITDFEKFARPEWLEKAVIYQIFPDRFNNGNPDLDPDQKLTWG
                     DPPDHYSLFGGDLAGIIEKLDYLKELGINAIYLTPIFESDSNHKYNIADYYKIDSNFG
                     DLQKAKELVKKAHDRNIKVILDAVFNHCDINFFAFQDLIKYGEKSKYKDWFFYDELPI
                     KTEKPINYVTFATQVKSMPKLNTGNQKVQDYLLNVVKYWMEELDIDGWRLDVSDEVHM
                     EFWRQFRKTVKKIKPQAVLIGEIWHSARKWLKGDRFDTVMNYPFNWAVMKFFGTNEIG
                     VEKFTSLIIKNYYHYREDTSNVLLNLLSSHDIPRIYEYCESKKQLKLAVLFMMTFPGI
                     PMVYYGDELGLKGGKDPDNRRCMPWDRVENNDLLDYYKKLIEIKKNISPLYKGDIRIV
                     LKDEANNVLVFKRRCKYKTVYIVINNSHISQNVTFKIERSGKYTELISDSVTESDNGT
                     LTVQMRDYEGKIFIHP"
     gene            complement(2418..3275)
                     /locus_tag="VKN64_09310"
     CDS             complement(2418..3275)
                     /locus_tag="VKN64_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HMA60477.1"
                     /translation="MYRRQDKKTKLLKHLIIWIVIAFALFPLVWTLSAALNPANTLIN
                     QELIPQNANFENFKYLFNNEQHPFPIWLWNSVKISFITATIAVSMTALAAYPFSRFKF
                     RGRRKGLFVILLIQIFPQMLAMVAIYLLFLNIQEVLPAFGLNTHPAIITIYLGGAIGV
                     NTWLMKGYLDTIPTSLEEAAYIDGASKFQAFYKIILPLARPILAVLFVLQFIATYSEY
                     ILASIILNSNEKYTLAVGLHLFISDMYANRWGVFSAAALLGAIPIVVLFMLVQKYIVH
                     GLIGGAVKG"
     gene            complement(3268..4827)
                     /gene="malF"
                     /locus_tag="VKN64_09315"
     CDS             complement(3268..4827)
                     /gene="malF"
                     /locus_tag="VKN64_09315"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HMA60478.1"
                     /translation="MSFWRNNYLYLAKIIFLSILDALAVWSATILFTEHKYIFLTILI
                     IVTAVINFIYLSKRAYPLRYIFPGTFFMILLVVYPIFYTVYVSFTNYGTGHILSKNQV
                     IEQLENRYYLPDDSRDFDYEAFKLPDQKKYAILLNDEQGNIYLAIDQQIEEITAGDPR
                     LKDPDGDGTIDEIVNYQKLSRPDLLKNLSTLQNLEIEFNDNILKMKSLNAFSLYRQQY
                     KYVESEDKIIDIKNNVEYFPQEGAFVSNEGEELLPGFRTFLGWENYLTLLTDERVQGP
                     FLRVFLWTFEWAILSVVTTFALGLFMAILLNDDTLRFRKIYRVILILPYALPAFISVL
                     IWRGLLNTEVGVVNQILEALSLNTIPWLQSIFWSRFALIVINLWLGFPYMMLISLGAL
                     QSISDDLYEAAAIDGATGWQRFKTITLPLILVSIGPLLISSFAFNFNNFNVIYLFNEG
                     GPPIVGAQTPAGGTDILISYTYRLSFESGRGADFGLASAVSIIIFMITAVITWFNFKY
                     TGALEEVKENV"
BASE COUNT         1640 a          898 c          832 g         1576 t
ORIGIN      
        1 aaatattcgg ccgaaccaga gtataatgtt ttcaaacatt atttagaatt acaggatgag
       61 tgggaaaata ttttaaactt tgctgatgat aattttcctg aactacagga tattttcaag
      121 gataccctgg taggtgaaac aaaagaaaaa gttgaggtta aaaaagatat taagagctat
      181 caaaatataa tcgacctacc tatctttaaa acagtatctg aagaagaatt agatgagtta
      241 cttagtatca gtcaatttaa aaattatgag ataggagaca aaattttaga gagtggagat
      301 agagatgatg ccctctttat aatgcaggaa ggcaaagtta aaattgttaa taataaagaa
      361 gaagaaaaag aaataaaatt agcagaactg gaaaaaggcg acttttttgg cttaacttct
      421 cttattgcca gtaaaagtgc aaattatcta gtagatgtag tcgcccagac tccagtaaag
      481 ctagttaaga ttaatagaga tgatattttt gactttttct ctgccaatca tgaatcaagc
      541 gtaaaactat tattatattt catgaaaaaa ttaaatgaac agttagaaga tctaacagaa
      601 atgtatacag atgtacaact taaaggtaga gatataagcc aaattatgga tgaataaata
      661 tcttaccttc atagtcacgc atctggactg ttaaagtgcc attgtcactt tcagtgactg
      721 aatcgctaat aagttcagtg tatttacctg atctttctat tttaaaggta acgttttgtg
      781 aaatatgact gttattgatc acaatgtaga ctgtcttata tttgcacctt cttttaaaga
      841 ctaagacatt attggcctca tctttaagta ctattctaat atctcctttg taaaggggag
      901 atatattttt tttgatttca attaattttt tataataatc taaaagatca ttgttttcaa
      961 ccctatccca gggcatacat cttctattat caggatcttt accacctttt aatcctaatt
     1021 catcgccgta atataccata ggaataccgg gaaaggtcat cataaataag acagctaatt
     1081 tcaattgttt tttactttca cagtattcat aaatcctggg gatatcgtgg cttgataaga
     1141 gatttaaaag tacattactc gtatcttccc ggtagtggta atagtttttt atgatgagag
     1201 aagtaaattt ttccacccca atttcatttg taccaaaaaa tttcatgact gcccagttaa
     1261 aggggtagtt catcacggta tcgaacctgt cacccttcag ccatttacgt gcactgtgcc
     1321 agatctcacc aattaaaacc gcctgtggtt taatcttttt gactgttttt ctgaattggc
     1381 gccaaaattc catgtgaacc tcatctgaaa catccagcct ccaaccgtcg atatcaagtt
     1441 cttccatcca gtatttaacc acatttaaaa gataatcctg tactttttga ttacctgtat
     1501 taagtttcgg catcgatttc acctgggtag caaatgttac ataattaatc ggtttttcag
     1561 ttttaatggg tagttcatca tagaaaaacc agtctttata tttagacttt tccccgtatt
     1621 tgattaagtc ctgaaaggca aagaagttaa tatcacagtg attaaaaaca gcatctaaga
     1681 tcacttttat attccgatca tgggcctttt ttactaactc tttggctttt tgcagatccc
     1741 cgaagttaga gtcaatctta tagtaatcag caatattgta tttatggttg gaatctgatt
     1801 cgaagatggg agtcaaatag atggcattga tacctaactc cttcagatag tccaacttct
     1861 cgatgatccc ggccaaatct cccccaaaaa gggaatagtg gtccggtgga tctccccaag
     1921 tcagcttctg atccgggtct aaatcagggt ttccattatt aaagcggtcc gggaatattt
     1981 gatagataac tgctttttcc agccattcgg gtctcgcaaa cttttcaaaa tcagtgataa
     2041 tagggtactg gaagaagcca gcttcatgtc cgcgcggcct gttttcaaaa aagccttttt
     2101 cattatacca gtatttattt ccctgataat cttctaaaag aaaatggtat cgaaagcgcg
     2161 gatcttctaa atgtatcttc acactataat aatcatgttt ttgatcactg cccacaactt
     2221 ccatctcttt actataaaca ggatctttat agaaatcgaa cttgggtcca tatattattt
     2281 taactttatt caaatcattt ttatccgtaa ccaatcttac ccgtaaggaa ttatcatcaa
     2341 gaggatagga tagattatct atactttcat ggtaaacagc tgctttattc aaagagaaca
     2401 cctccttatt ttatatatta acccttgaca gctccaccga tcagtccatg tacaatatat
     2461 ttttggacta acataaagag gacaacaatt gggattgcgc ccaagagagc ggcagctgaa
     2521 aatactcccc agcggttggc atacatgtcc gagatgaaga ggtgcagtcc aacagccagc
     2581 gtatattttt cattagagtt taaaatgata ctggctaaaa tatactccga gtatgtggca
     2641 ataaactgta aaacaaataa tacagccagg atgggccggg ctaatggtaa tattatcttg
     2701 taaaaggcct ggaacttact cgccccatct atataagccg cttcttcaag agaagttggg
     2761 atagtatcta aataaccttt cattaaccag gtattaaccc caatcgcccc acctaaatag
     2821 atagtgataa tggcaggatg ggtattaaga ccaaaggctg gtagtacttc ctgtatattt
     2881 aaaaataata gatagattgc aaccatggcc agcatttgag ggaatatttg aattaaaaga
     2941 attacaaaca gtccttttct acgtccccta aatttaaatc tggaaaatgg ataggccgct
     3001 aaggctgtca tgctaacagc aatcgtagct gtgataaaag atatcttcac actgttccac
     3061 agccagatag gaaagggatg ctgttcatta ttaaataggt atttaaagtt ttcaaaatta
     3121 gcattttgtg ggattaattc ctgattaatt aatgtattag caggatttaa ggcggctgat
     3181 aatgtccaca ctagtggaaa tagggcaaaa gcaataacta tccagattat taaatgtttt
     3241 agtaattttg ttttcttatc ttgacgtcta tacattttcc ttcacctcct caagagcacc
     3301 tgtatattta aagttgaacc aggtaattac agctgtaatc ataaatataa taatacttac
     3361 agcactggcc agaccaaagt cggctccgcg accactttca aaggacagac ggtaggtata
     3421 ggatattaag atatctgtac cgccggccgg ggtctgagct cccactatcg gtggtccacc
     3481 ttcattgaaa aggtaaatta cattaaaatt attaaagtta aaggcaaaag aagatattaa
     3541 gagtggacca atactaacta agatcagagg caatgtaata gttttaaaac gctgccagcc
     3601 tgtagcacca tctatagcgg ccgcctcata caagtcatcc gaaatacttt gtaaggcccc
     3661 cagactaatt aacatcatat aggggaaacc cagccataaa ttaataacaa tgagtgcaaa
     3721 tctggaccaa aaaatactct gtaaccaggg tattgtattt aaggataaag cttctaaaat
     3781 ttgatttact acacccacct cggtatttaa aagaccccgc caaattaaga cagagataaa
     3841 ggcaggtaag gcataaggca agatcagtat taccctgtat atctttctaa acctcaaagt
     3901 atcgtcattt aacaatatag ccataaataa gcccaaggca aaggtagtaa ctacacttaa
     3961 gattgcccat tcaaaggtcc ataaaaatac tcttaagaaa ggtccttgta ccctttcatc
     4021 ggtaagtaat gtcaaatagt tttcccagcc taagaatgtt ctaaaccctg gtaaaagttc
     4081 ttcaccttca tttgaaacaa aggcaccttc ttgggggaaa tattcaacat tattttttat
     4141 atctataatt ttatcttcac tttcaacgta tttatactgc tgtctataaa gagaaaaggc
     4201 attaagactt ttcattttca agatattgtc attaaattct atttctaagt tttgcagtgt
     4261 tgataaattt ttcaatagat caggtcttga gagtttttga taatttacta tttcatcaat
     4321 agtaccatcc ccatcaggat ccttcaaccg gggatcaccg gctgttattt cttcaatttg
     4381 ttggtctatt gccagatata tattaccctg ttcatcattt aaaagtattg catatttttt
     4441 ctgatcaggt agtttaaaag cttcatagtc aaagtcccgc gaatcatccg gcaaataata
     4501 tctgttttcc aattgttcaa ttacctggtt tttcgataaa atatgtccgg ttccatagtt
     4561 ggtaaaggaa acataaacag tataaaagat aggataaacc acaagcagta tcataaaaaa
     4621 tgtaccggga aatatatatc tgagagggta agctctttta gatagatata tgaagttaat
     4681 aactgctgta actattatta agatcgttaa aaagatgtac ttatgttcgg taaataagat
     4741 agtggcactc catactgcta gtgcatctaa tatactcaaa aatataattt ttgctagata
     4801 taaataatta tttctccaga agctcacaat tcgccctcct tttcttttca attataaaaa
     4861 aactggtgcc ccacattgga caccagtttt gtaattacaa ttgatctata tattattgac
     4921 tagcttcaat tgctttttcg atttgt
//
LOCUS       DATGCJ010000204         5302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_163370, whole genome shotgun sequence.
ACCESSION   DATGCJ010000204 DATGCJ010000000
VERSION     DATGCJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163370"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..869)
                     /locus_tag="VKN64_09320"
     CDS             complement(66..869)
                     /locus_tag="VKN64_09320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60479.1"
                     /translation="MSKSKSNLIYDVNEHVIEIYDQIETETHDIEFILDYIKDKNIER
                     ILEPFCGNGRLLISLAEEGYQVVGMDLSFNMLDSLKKKIKDKEKRIKDNIKLIQADIL
                     EYKWPENFDLLILGCNCLFELATPEQQVKIIQKAYNSLKEDGLLFIDNDNMEGKLDES
                     WCEIGREKEIFPNGICQHGIEFKGTYKTTYINKDKRIWRAERKVEINYPDGKKDNKRL
                     NQQKHPVSAPEVKNWLIDTGFKIIKVYSDLEKRKEFTKGANRATFLAKK"
     gene            complement(891..2282)
                     /locus_tag="VKN64_09325"
     CDS             complement(891..2282)
                     /locus_tag="VKN64_09325"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMA60480.1"
                     /translation="MAIVFIQSSNLKAVEKEQINAMIDRHIDDIAIPGIAVGIIKDDK
                     EVYYDTKGVNGQGNKLNSKTPMFIGSVSKSLTALAVMQLVEDNLISLDEPVKKYITYF
                     EVSDPKLSEEITVRDVLNQTTGLARKNSIPSSDYDFTLKERVKELADMRASRNRGEFN
                     YLNDHYNILGLLIEEVTGSSYAEYMEENVFKPIGMINTTADIQTIRDKDVYGYTNIFG
                     FSKSIKQSVPRYDIPSGYILSNLEDMYRYLEFLIDPDKSIISKESIDIMRTTAGNSTY
                     GMGWHIRKIDGQRVVEHSGAVPGFSSHLAVIPATNSGYLYIMNKNHLIHNFVNVYNRI
                     NGNMLKAALGDSSFNYFPSIWVIRIFSLVLLILTAKDLWNTKELFHKTKDKKGWVKEA
                     IKSLLLILFLTLGLSFILRNMLGLAVDLKIMFAYVPDYTALLVIDILVQVIRLAISLF
                     YIFKTKEKHAFQE"
     gene            complement(2394..3653)
                     /locus_tag="VKN64_09330"
     CDS             complement(2394..3653)
                     /locus_tag="VKN64_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60481.1"
                     /translation="MEEKELEKKLVQLLGKQKLFLWDNYALGQDRELNEVDQSIYNLL
                     KNIDPRDYFQGRKKEHFERLYYKLMIENDPEIAVLKNKLDNTENYNNKLDKNVVDYKI
                     KIAKRKKEDVIQLMKLRNKKARNWGFNSYPDFNFYYEELHKETVIKMIKKHLEDNINK
                     ANDLINEYDLTWENWFERLRNIGSIDRQESSYHFNCLLKTLDLNINSEDITFKTKKDG
                     ISGMTFNISIPDDIRILSKPITSPMTGRVFFHECGHAINYATANGEGIYNIYNPFHDE
                     AVAIIFENIGIKSCMNKKEQKISEEVKFLETIRCSISFLFEMEIWKKPDQAEELFLKY
                     QSLLNMKPNKKEMWTLDSFRYIDPVYIQNYVLGEIYANKIIDSLENCYSDNYIKWGQE
                     IHNYFSKDGMEKSFKTKYKEFNFKLND"
     gene            complement(3717..4346)
                     /locus_tag="VKN64_09335"
     CDS             complement(3717..4346)
                     /locus_tag="VKN64_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMA60482.1"
                     /translation="MYKHIIWDFDGTLFNTYPGVAHTFLKIMKEKYEKDFQYEKVLEW
                     SKFSLKFCAENLSEDINTDKESLIESFHAEYMSNGAPIEEPPFKGAKEICELVNERGG
                     LNLLVTHRGEMTLKRLLEKNNMREYFKELISRDDEFPRKPDPAAFLYLIDKYNLTREN
                     TLAVGDREIDVDAAHNAGITACYFNPTGSKYEESDYNIESLLALKDIIF"
     gene            complement(4362..>5302)
                     /locus_tag="VKN64_09340"
     CDS             complement(4362..>5302)
                     /locus_tag="VKN64_09340"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HMA60483.1"
                     /translation="DSAINKFKELGLNVIETESVRKNNDNLVSAPPEVRAKEFNELYF
                     NKEVRAIIPPWGGEFLMEMLPHLDFKKLKETEPKWILGFSDTSTLLFTLTLKANLATA
                     HGPNFLDFGNNPVDESVLESLKILQKEKGASFVQESLEYYQEEWSEVTEDTFPPYNLT
                     EKVDWKILGEDTQAQFKGRLIGGNFDVICKLIGTPYDKVREFINQYKEDGFIWYFENC
                     EMDSTDVRRTLWQMQMNDWFENCNGLLYGRVAGYNALKGYTCVDALSSIKNELDIPIV
                     YDVDLGHKPPQLTFINGAYAEVTVEENHEKVLQKLI"
BASE COUNT         1638 a         1011 c          669 g         1984 t
ORIGIN      
        1 caataaaata ggagttttta attagatttt gtatcttgtt atccacttag atcacctttg
       61 atatattatt ttttagccag aaaggttgcc cggttggctc ctttagtaaa ctctttcctt
      121 ttctcaagat ctgaatatac cttgattatt ttaaagcctg tatctattaa ccaatttttt
      181 acttctgggg ctgaaacagg gtgtttttgc tggtttaatc ttttattatc ttttttgcca
      241 tctggatagt tgatctcaac cttacgctcc gctctccaga ttctcttatc cttatttata
      301 taggttgtct tatatgttcc tttaaattca attccatgct gacagatacc gtttggaaat
      361 atttcttttt ccctacctat ttcacaccag gactcatcta acttgccttc catgttatca
      421 ttatcaatga ataataaacc gtcttctttt aaagaattat aggctttctg gataatcttc
      481 acttgctgct cgggagttgc taattcaaaa agacagttgc agcctaatat taataaatca
      541 aagttttccg gccatttata ttcaagtata tcagcctgga ttagctttat attgtcttta
      601 attctttttt ctttatcctt aatcttcttc tttaggctat caagcatgtt aaaagaaagg
      661 tccataccga caacttgata accttcttca gctaaagaga ttagcaatcg accattcccg
      721 cagaagggtt ctaatattct ctcaatattt ttatccttga tatagtctaa gataaattct
      781 atatcatggg tttcagtttc aatttgatca taaatttcaa taacatgttc atttacatca
      841 tatattaaat tggatttgct tttagacatt ataagatcac ctatatttaa ttattcttga
      901 aatgcgtgct tctcttttgt tttaaatata taaaataaag aaattgctaa tctaataact
      961 tgaactaaaa tatcaataac taataatgct gtataatccg gtacataggc aaacattatt
     1021 ttaagatcta ctgctaaacc cagcatgttt ctcaggataa aagacaatcc gagggttaaa
     1081 aataaaatta atagtaagga ttttattgct tcttttaccc agcccttttt atcttttgtt
     1141 ttatgaaata attctttagt attccataaa tctttcgctg ttaatattaa gagcactaag
     1201 gaaaatatcc gaataaccca aatagaaggg aaataattga atgagctgtc ccctaaggct
     1261 gctttcagca tattaccatt gattctatta tacacattaa caaagttatg gataaggtga
     1321 tttttattca tgatataaag atacccacta tttgtggccg gaattacggc caggtgtgag
     1381 ctaaaaccgg gcactgctcc actgtgttca actacccttt gaccatcaat ttttcttata
     1441 tgccagccca tcccataggt gctattacca gcggtggttc tcattatatc aattgattct
     1501 tttgaaatta tacttttatc aggatcaatt aaaaactcca aatatctata catgtcttct
     1561 aaattggata aaatatagcc ggaagggata tcataacggg gcacagactg tttaatactt
     1621 ttagaaaaac caaaaatatt ggtatatcca tagacatctt tgtctctaat agtctgaata
     1681 tcagcagtag tattaatcat accaattggt ttaaaaacat tttcctccat atactcagca
     1741 tatgaactac ccgtaacttc ttctattaat aaacctaaaa tattgtaatg gtcattaaga
     1801 tagttaaatt cacccctatt cctgctggct cgcatatctg ctaattcttt caccctctct
     1861 ttaagagtaa aatcataatc tgaactggga atgctatttt ttctcgccaa acctgtagtc
     1921 tggtttaata catctctaac agtaatttcc tcagataact tcgggtcgga cacttcaaaa
     1981 tatgttatgt attttttaac aggctcatct aaactaataa ggttatcttc cacaagctgc
     2041 atcacagcta aagctgtcag tgacttagaa actgaaccga tgaacatagg ggttttgcta
     2101 tttaatttat taccctgtcc gttaactccc ttggtatcat aatatacttc tttgtcatcc
     2161 ttgataattc caactgctat acccggtatt gcaatatcat caatatgccg gtctatcatc
     2221 gcattaattt gctctttctc aactgctttt aaattgctgc tttgtataaa tactattgcc
     2281 attattatta ctagtattat agtaaattta ttatattgag tatatttaat attaatctcc
     2341 tcctgaaaat tatattaatc atattaaaat aaaaccttat ccaaaaatta tttttagtca
     2401 tttaatttaa aattaaattc tttatattta gttttaaagc ttttttccat accgtctttt
     2461 gaaaaatagt tatgaatttc ctgtccccat ttaatataat tgtcactata acaattctct
     2521 aaactatcaa taattttatt ggcataaatt tctccgagca cataattttg aatataaacc
     2581 gggtcaatat atctgaaaga atcaagtgtc cacatttcct ttttattagg tttcatgtta
     2641 agtaaagatt ggtatttcaa aaataattct tcagcctgat caggcttctt ccagatttcc
     2701 atctcaaaca agaaactaat actacatcgg atggtttcta aaaactttac ctcttcagat
     2761 atcttttgtt cttttttatt catacacgat tttatcccga tattttcaaa tatgattgca
     2821 actgcttcat catgaaaagg attataaata ttataaatcc cctcaccatt ggcagtggca
     2881 taatttatag catgtccgca ttcatgaaaa aacacccgtc cggtcatggg tgaagttatc
     2941 ggtttagata aaatcctaat atcatccgga atcgagatgt taaaggtcat acctgatatc
     3001 ccatcttttt tagttttaaa tgtgatgtcc tcactattaa tatttaaatc tagtgttttt
     3061 aacaaacaat taaagtgata agaggactct tgtctatcaa tacttcctat attccttaat
     3121 ctctcaaacc aattttccca ggttagatca tattcattta ttaaatcatt tgctttatta
     3181 atattatctt ctaaatgttt ttttatcatt tttataacag tttctttatg cagttcttca
     3241 taataaaaat taaaatctgg ataagaatta aagccccaat ttcttgcctt tttatttctc
     3301 aatttcatta actgaattac atcttctttt tttcttttag caatttttat tttataatca
     3361 acaacatttt tatctaactt attattataa ttttcagtat tatccagttt attctttaga
     3421 acagctattt ctggatcatt ttctatcatt aatttataat atagacgctc aaaatgttcc
     3481 ttttttcgtc cctgaaaata gtctcgggga tcaatatttt ttaaaagatt atatatagat
     3541 tgatcaactt catttaactc tctatcttgg cctaaagcat aattatccca aagaaataat
     3601 ttttgttttc ctaataattg aactaacttt ttttcaagct ctttctcttc cattaaaatc
     3661 tcctcattat ctatcatatt aaaacagcat tatattaaat aaaatataaa tatctattaa
     3721 aatattatat ctttaagtgc taacaaactt tcaatattat aatcgctttc ctcatatttg
     3781 ctgcccgttg ggttaaaata gcaggcagta atacctgcat tatgggcagc atcaacatct
     3841 atttccctgt ctcctaccgc taaagtattt tctctggtta agttatactt atcgattaga
     3901 tacagaaaag cagctggatc cggcttacgg ggaaattcat catccctgga aatcaattct
     3961 ttaaaatatt ccctcatatt atttttttct aatagtcttt tcaatgtcat ttcaccccga
     4021 tgggtaacta ataagtttag tcctcccctt tcattaacca attcacagat ctctttagct
     4081 cctttaaaag gaggttcttc aatcggggct ccattgctca tatattccgc gtgaaaggat
     4141 tcaattaatg actccttatc ggtgtttata tcctcagata aattttcagc acagaatttg
     4201 agggaaaact tgctccactc caggaccttt tcatattgaa aatctttttc atatttttct
     4261 ttcattattt ttaaaaaagt atgagctaca ccgggatatg tattaaataa tgtaccatca
     4321 aaatcccaaa taatatgttt atacatataa tagaccctca cttatataag tttttgtaga
     4381 actttttcat ggttttcttc gacagtaact tccgcatatg caccattaat aaatgttaac
     4441 tgcggtggtt tatgacctaa atctacatca tagactatag gtatatctaa ttcattttta
     4501 attgatgaca aggcatcaac acaggtatat cctttaagag cattataacc tgcaacacgt
     4561 ccgtataaaa ggccattaca gttttcgaac caatcgttca tctgcatctg ccagagggtt
     4621 ctcctaacat cggttgaatc catctcacag ttttcaaaat accagatgaa accatcttct
     4681 ttatattgat taataaattc cctcacctta tcataaggag ttccgataag tttacagatt
     4741 acatcgaaat tgcccccgat cagacgcccc ttaaactgtg cttgagtatc ttctcccagg
     4801 attttccaat ctactttttc tgtcaaatta tagggcggga aagtatcctc agttacttcc
     4861 gaccattcct cttgataata ttctaaactt tcctgtacaa atgaagctcc cttctctttc
     4921 tgcaatatct ttaaactctc taaaactgat tcatccaccg gattatttcc aaagtctaaa
     4981 aagttaggtc catgggcagt tgctaaattt gcctttaaag taagagtaaa tagaagggta
     5041 cttgtgtcag aaaaacctaa aatccatttt ggctcagtct cttttaactt tttaaaatcc
     5101 aagtgaggta gcatttccat caagaactct ccaccccagg gcggtattat agcccttact
     5161 tccttattaa aataaagttc attgaattct ttggctctta cctcgggagg agcacttact
     5221 aagttatcat tattctttct gactgattcc gtctcaatta cattcaatcc cagttcctta
     5281 aacttattaa tagcactatc ta
//
LOCUS       DATGCJ010000205         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_343560, whole genome shotgun sequence.
ACCESSION   DATGCJ010000205 DATGCJ010000000
VERSION     DATGCJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343560"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VKN64_09345"
     CDS             <1..865
                     /locus_tag="VKN64_09345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMA60484.1"
                     /translation="AFSQKELDQFKENIKLQHKFDIPSRLLTVKEAKEIVPQLNTTGV
                     SGAAFCPTDGHANPFRVNQAYVEAAERKGVEFYTYTKAEDIVVKNGRIKKVVTDKGDI
                     ETNRVVNAAGGFSKEVGKMAGIDIPTKSERHEILVTEQVNSILKPMVMSFSYNIYCQQ
                     TPDGSFVMGYGDPDEPASHNIRASWQFLEEMSRKATYLLPFLKDIRVIRQWAGLYNVT
                     PDAQPILDESDEVPGYFMAVGFSGHGFMIAPMTGILMAEMITGEKLSIDIELDLKRFD
                     HGRLILEPSVV"
     gene            887..2521
                     /gene="pruA"
                     /locus_tag="VKN64_09350"
     CDS             887..2521
                     /gene="pruA"
                     /locus_tag="VKN64_09350"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMA60485.1"
                     /translation="MSANDIFSLKRPTNEPILSYEPGSEEKIELKTKLKELKNNPVEI
                     PLIINGKEVKTGKTKEIRAPHNHDIVLGKYHMAGKEEVNQAIVASLEAKKEWENTSWE
                     KRASIFLKAASLLAGPKRAEVNAATMLCQSKNVFQAEIDSACELIDFLRFNSYFMSEI
                     YQNQPSSTDNILNRMEYRPLEGFIFAVPPFNFTSIGGNLPTAPALMGNTVLWKPASTA
                     VYSNYFFMKLLMRAGLPAGVINFIPGKGSEIGPLVTERPELAGIHFTGSVNTLNQMYK
                     EVGSNVEKYDTYPRIVGETGGKDFIFAHHSADKDELITALVRGAFEYQGQKCSAASRS
                     YIPNNLWSELKEELIEQTEGLKMGPVDDFTNFINAVIDEAAFDKISGYIDYAKKADDA
                     EVIAGGGYDKSDGYYIEPTIILTDNPKFKTMREEIFGPVLTIYVYNKGDFTETLKLCD
                     ETSPYGLTGSIFAKDRKAVETAEKVLKHAAGNFYINDKPTGAVVGQQPFGGARKSGTN
                     DKAGSQWNLMRWVSPRAIKENLNPPTDYSYPFMKEE"
     gene            2702..2908
                     /locus_tag="VKN64_09355"
     CDS             2702..2908
                     /locus_tag="VKN64_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA60486.1"
                     /translation="MPNQGKVKITHPERCKGCEICVMYCPTDVLEVKKGQITITDIDS
                     CVGCYNCELSCPDFVLEVVEEDEK"
     gene            2898..>3106
                     /locus_tag="VKN64_09360"
     CDS             2898..>3106
                     /locus_tag="VKN64_09360"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMA60487.1"
                     /translation="MKNKPKLLQGDEAVVEGAIAAGARFFAGYPITPASEIAEGLAEK
                     LPVNGGKFIQMEDELASMACVIGAS"
BASE COUNT         1009 a          548 c          691 g          858 t
ORIGIN      
        1 agcattttct caaaaagaat tagatcagtt taaagaaaat attaaattac agcataagtt
       61 tgatattccg tcccgcctgt taactgttaa agaagcgaaa gaaattgtac cacagttaaa
      121 tacaaccggt gtaagcgggg ctgccttctg tccgaccgat ggacatgcta atccctttcg
      181 ggttaatcag gcttatgtag aagctgccga aagaaaaggg gtagaatttt atacttatac
      241 caaagcagaa gatatcgtag ttaaaaacgg ccggattaaa aaagtggtta ccgataaagg
      301 ggatattgaa acaaaccggg ttgttaatgc agccggtggc ttttccaaag aagtcggcaa
      361 gatggcgggc attgatattc ctaccaaatc tgaaagacat gagattttgg taaccgaaca
      421 ggtcaattcg atcttaaaac cgatggtcat gtccttttca tataatatat actgtcagca
      481 gaccccggat ggtagttttg tgatgggtta tggagatcct gatgaaccgg ccagtcataa
      541 tatcagagcc agctggcagt ttttagagga gatgtcgcgg aaagcaacct atttactgcc
      601 ttttttaaag gatattaggg ttatcaggca gtgggccgga ctatataacg tgacaccgga
      661 tgcgcaaccg attttagatg aatcagatga ggtcccaggt tacttcatgg cagtcggatt
      721 cagcggacac ggctttatga ttgcaccgat gacagggatt ttgatggcag aaatgattac
      781 tggtgagaag ttaagtatcg atattgaact tgatctaaaa agatttgatc acggacgatt
      841 aatattagaa ccgtcagttg tttaaaatta ttaggaggga tttaagttga gtgcaaatga
      901 tatttttagt ctaaaaagac ctaccaatga accaatttta agttatgaac caggtagtga
      961 agaaaagatt gaactaaaaa ctaaactaaa agagctaaaa aataatccag ttgaaatacc
     1021 tctcattatt aatggtaaag aagtaaaaac ggggaagaca aaggaaatta gggctcccca
     1081 taaccatgat atagtattag gtaagtatca catggcaggc aaagaagaag ttaatcaggc
     1141 catagtggct tctttagagg ctaaaaaaga atgggagaat acttcctggg aaaaaagagc
     1201 ttccatcttt ttaaaagcag cttctttatt ggccggtccc aaaagagcag aggtaaatgc
     1261 ggccactatg ctctgtcaga gcaaaaatgt tttccaggcc gagatagact cagcctgtga
     1321 gttaattgat tttttacgct ttaattcata ttttatgtcc gaaatctatc aaaaccaacc
     1381 cagttctacg gataatattt taaacaggat ggaatataga ccattagaag ggtttatctt
     1441 tgcagtacca ccctttaact ttacatctat cggtggtaat ttgccgaccg cacctgccct
     1501 aatgggtaat actgttttat ggaagccggc ttcaaccgcc gtctattcta actacttctt
     1561 tatgaagtta ctaatgaggg caggactacc ggccggagta attaacttta ttcccggtaa
     1621 aggatctgaa attggcccac tggttactga acgacctgaa ttagccggta ttcactttac
     1681 aggttcagtt aatactttaa accagatgta caaagaagtg ggctctaatg tagagaaata
     1741 tgatacctat cccagaattg tcggtgaaac cggtggtaaa gacttcatct ttgcccacca
     1801 ttcggccgac aaggatgaat taattaccgc acttgtcagg ggtgccttcg aatatcaggg
     1861 acagaaatgt tcagctgctt cccgctccta tatacccaat aacctctggt ctgaacttaa
     1921 agaggagtta atcgaacaga ccgaaggttt aaagatggga ccagttgatg acttcactaa
     1981 cttcattaat gccgtcatcg atgaagcggc ctttgataag atctccggct atatcgacta
     2041 tgctaaaaaa gctgatgatg cggaagttat tgccggaggc ggttatgata aatcggatgg
     2101 ctactatata gaaccaacta ttatcttaac tgacaatccg aaatttaaaa ccatgcggga
     2161 agaaatcttt ggcccggttt taaccatcta tgtatataat aagggggact ttacagagac
     2221 ccttaagctt tgtgatgaga cttctcctta tggattaacc ggtagtatct ttgccaagga
     2281 ccgaaaagca gtcgaaactg cagaaaaagt actaaaacat gccgccggta acttctatat
     2341 taatgataaa ccgaccggag ctgttgttgg tcaacagccg tttggtggag ccagaaaatc
     2401 aggaacaaat gataaggcgg gcagtcagtg gaatttaatg agatgggtct ctccacgggc
     2461 tattaaagag aacttaaatc caccaaccga ttattcctat ccatttatga aagaagaata
     2521 aggctttaaa tactgaatat tcaggggtga gaacatttcg ctcctgaata tttttttgac
     2581 caaattaaag gtttttaaaa tttgatattg aaataatgat ttggttgatg tattattcaa
     2641 ctataagttt atacataagt taataaagat aataatacca ataatttaag gagctgattt
     2701 aatgccgaac caaggtaagg ttaaaatcac tcatccggaa cgttgtaagg gatgtgaaat
     2761 ctgtgttatg tattgtccca cagatgtact ggaagttaag aaaggacaaa taaccattac
     2821 cgatattgat agttgtgttg gctgttataa ttgtgaacta agttgtccag acttcgtact
     2881 agaggtggtg gaagaagatg aaaaataaac caaaattatt acagggagat gaagcagtag
     2941 tagagggagc aatcgcggcg ggagccagat tttttgccgg ttatccgatc acacctgcct
     3001 cggaaatcgc agagggttta gctgaaaagc tacccgtaaa cggcggcaag tttattcaaa
     3061 tggaagatga actggccagt atggcctgtg tgataggtgc ttcttt
//
LOCUS       DATGCJ010000206         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_343702, whole genome shotgun sequence.
ACCESSION   DATGCJ010000206 DATGCJ010000000
VERSION     DATGCJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343702"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="murI"
                     /locus_tag="VKN64_09365"
     CDS             complement(<1..574)
                     /gene="murI"
                     /locus_tag="VKN64_09365"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMA60488.1"
                     /translation="MEDTKQAIGMLDSGVGGLTVAREVMNTMKAEKIIYFGDTLHLPY
                     GPKPKEDVRKYVYRIIEYFKTRNVKTIILACNTATSMLLKETRRTVKLPLFGTIDGVA
                     KSVKEATKNNKIGVIGTKGTIKSRAYQEELKKESNNFEIHAKACPPFVNLAEEGHFKG
                     AETKKVIHKYLDPLREKGIDTLILGCTHYPY"
     gene            complement(608..1114)
                     /locus_tag="VKN64_09370"
     CDS             complement(608..1114)
                     /locus_tag="VKN64_09370"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HMA60489.1"
                     /translation="MKNKLFKLILLLIIFVLVIFAVYYFIDRTNLIVKEKTVNLYFAT
                     TDAMYLETEERTIKGRNLYKEAVNALITGPTDSDLAKTIPEGVKVLNISKKNDTIRVD
                     FSEEIVKNHWGGSAGERLTVYSIVHTLAQFKGVKQVKFLIEGEEVETLVGHMDLSVPL
                     APNQEIVK"
     gene            complement(1126..3351)
                     /locus_tag="VKN64_09375"
     CDS             complement(1126..3351)
                     /locus_tag="VKN64_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HMA60490.1"
                     /translation="MNLKKYTVIFLLLILILGSQAVLSADMPKIYINGENKTAQLDPV
                     KSNGAILVRSKALAQSIGAKLKWLGSINTLSITKNGKTVKMMVESPYIQINSRTIRTE
                     SPMVIKDNYTYIPFTNIMDVFGFLIEHERISNSYYLFKPESHLKEIVWGPNRQSLILK
                     MDNIAPYRLRLTDDPKKLIVEIEKAALESNFRDKISDKNYYLKSRRIENQATLQIVLT
                     SKYPLPINSDEVISEENGNLLIQFMPQIASINWKQDNTLEIKTNGDINKPEVSFLEEP
                     RRMVLDIPGVLLSEFEKNLAENKWIKDVRVSQFQFEPTILRVVLDLKEKKYLNIVNQD
                     DSEKIVLKPAQRARVFDLAYKDHQIMFKTDAPIEPDLFTLQNPSRLVVNLFNTYREDN
                     MADEIKVKENIVKKVRVGRFDSQIVRIVADLSEMTAYEHEQEQYSKNIYIHRISFANK
                     FDDIELLDQNMYTNININVSGKVNYEVKKFSYPHRLVVDISGKEITPDEVKLPKPVGI
                     IKEMRMGRFELDDETVTRVVFELSKYFNHNVVSENPDNKINIALAKSEIQSENNLIVL
                     DAGHGGFDPGAVGSNLQEKEVNLKIVERLESILKSHGFKVMLTRKDDTFISLNKRVKI
                     ANNNGARIFISVHNNSSSKNTSNGTETFIAPNKTGDSLLLAENIQKQLIDRLELVDRG
                     VKKEHLYVVKYTEMPAVLVEVAFLSNPREEKLLQVDGFLTEAAVAISQGIFDYFEKFK
                     D"
     gene            complement(3487..3768)
                     /locus_tag="VKN64_09380"
     CDS             complement(3487..3768)
                     /locus_tag="VKN64_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HMA60491.1"
                     /translation="MFVGVITVELQIPMAQSLKDKRSIIKSLTDKCKNKFNVAVAEVD
                     NKDLWKNATLGLVTVTSDRRYLDKVINKIISFIEDFPGVYLYNHTVDYY"
     gene            complement(3773..4375)
                     /locus_tag="VKN64_09385"
     CDS             complement(3773..4375)
                     /locus_tag="VKN64_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA60492.1"
                     /translation="MTRKYELFDDLEDKKSRICVAAINLFSKKSFNQTTVEEIARETG
                     VGKGTFYNYFESKEYLLNFLLDYGTVKLINYVKDSIKEGMSPKDKMETAIDSHLQYFG
                     DNHDYFVFYLREMWSYREGLEDQILKLKEDYITIFEDIIKEGKKEGLFKDIDTETIGS
                     GLFGLLSVASSHWALFSSEFPIENIDRSVKEVFFNGLLRR"
BASE COUNT         1428 a          948 c          698 g         1744 t
ORIGIN      
        1 aataagggta atgagtacaa cctaaaatca aagtatctat ccccttttct cttaaagggt
       61 ctaaatattt atgaattacc tttttagttt cagctccttt aaaatggcct tcttcagcta
      121 aattaacaaa aggtgggcag gctttagcat gaatttcaaa gttattactt tcttttttta
      181 actcttcctg ataagccctg ctttttatag tccctttggt acctataact cctatcttat
      241 tattcttggt agcttctttg accgacttag ctaccccatc aatagtacca aatagcggta
      301 gcttaacagt tcttctagtt tcttttagta acatagaagt agctgtatta caggccagga
      361 ttattgtttt tacatttctt gttttgaagt actcaattat cctatataca tattttctaa
      421 catcttcttt tggtttgggt ccatagggaa gatgtaaagt atcaccaaaa taaatgatct
      481 tttctgcctt catagtattc attacttcgc gcgctacggt taaaccacct acgcctgaat
      541 ctaacatacc aattgcttgt tttgtatctt ccattttttt cactcctgat agattaatta
      601 catgatttta ttttacaatt tcctggttcg gggccagcgg tacactgaga tccatgtgtc
      661 ctaccagggt ctcgacctct tctccttcta ttaaaaattt aacctgttta actcccttaa
      721 attgggccag tgtgtgaaca atcgagtaaa ctgttaggcg ttctcccgct gaaccacccc
      781 agtggttctt cacaatttct tcactaaaat caactcgtat tgtatcattc ttcttggata
      841 tattcaaaac tttaacccct tcgggtatag ttttggctaa atcagaatca gtaggacctg
      901 taataagggc attgacagct tccttataaa ggtttctacc tttaatggtt ctttcttcag
      961 tttcaagata catcgcatct gttgtggcaa aataaagatt gacagttttt tctttcacta
     1021 taagatttgt tctatcaatg aaatagtata ctgcaaatat tacgagaaca aatatgataa
     1081 gtaataatat gagtttgaat agtttatttt tcattgactt ccacctcaat ccttaaactt
     1141 ttcgaagtag tcgaatatgc cctggctgat tgcaacggca gcctctgtta agaacccatc
     1201 tacttgcagc agtttttctt cccttggatt agataaaaag gctacttcta ctaaaactgc
     1261 aggcatctcc gtatatttta caacataaag atgttctttt ttaactcccc tatcaactaa
     1321 ctccagtcta tctataagct gcttctgtat attttcagct aagagcagac tgtctcccgt
     1381 cttattaggg gcaataaagg tctcggttcc attggaagta ttcttacttg aggagttatt
     1441 atgaacactt ataaatattc tagccccatt attattagct attttgactc ttttatttaa
     1501 ggatataaag gtgtcatctt ttctggttaa cattactttg aaaccgtggc ttttaagaat
     1561 actttctaat ctctctacta tttttaagtt gacttctttt tcctgtaagt tactgccgac
     1621 agccccgggg tcaaaaccac cgtggccggc atctaatacg atcaagttat tttcactctg
     1681 tatttcggac ttggctaagg ctatatttat tttattatct ggattttcag atactacatt
     1741 atggttaaag tacttgctca attcaaatac tacccttgtt acagtttcat catccaattc
     1801 aaatcttccc attctcattt cttttataat accgaccggt tttggtagtt tgacttcatc
     1861 cggtgtaatt tcttttccgc taatatcaac aactaatcga tgtgggtaag aaaacttttt
     1921 aacctcataa ttaaccttac cacttacatt gatatttata ttggtataca tgttttgatc
     1981 tagaagttca atatcatcaa atttattagc aaaacttatc cgatgaatat aaatgttttt
     2041 actatactgt tcctgttcat gttcataagc tgtcatctcg cttaaatcag ctacaatcct
     2101 aacgatttga ctatcaaatc tacccactct tactttttta acgatatttt ctttaacctt
     2161 tatctcatct gccatgttat cttcccggta ggtattaaag aggttaacta ccaggcgtga
     2221 cggattttgt aaagtaaata aatccggttc aatcggggca tcagttttaa acataatttg
     2281 atggtcctta taagctaggt caaagaccct tgctctttga gcaggtttta agactatttt
     2341 ttcactatca tcctgattta caatattaag gtacttcttt tcttttaaat ccagtacaac
     2401 acgtaaaata gtaggttcaa actggaactg actgaccctt acatctttaa tccatttatt
     2461 ttcagcaagg ttcttttcga attcacttaa taatactccc ggtatatcaa gcaccattct
     2521 tctaggctct tctaaaaaag aaacttccgg tttatttata tcaccatttg ttttaatttc
     2581 taaggtatta tcttgtttcc agttgatgga agcgatctgg ggcatgaact ggattaaaag
     2641 attaccgttt tcttcactga tcacttcatc agaattaata ggtaaaggat acttactggt
     2701 tagtacaatc tgtaaagtag cttgattttc tatccggcgg cttttaaggt aataattttt
     2761 atcggaaatt ttatccctga aattagattc taaggctgct ttttcaattt caactattaa
     2821 ttttttagga tcatctgtta aacgcagtct atagggggca atattatcca tctttaaaat
     2881 caaagactga cggtttggcc cccagacaat ctcttttaaa tgagattctg gtttaaacag
     2941 gtaataggaa ttacttatcc gttcatgttc aattaaaaat ccaaagacat ccatgatatt
     3001 agtaaaagga atatatgtat agttatcctt gattaccatc ggtgactccg ttcttatcgt
     3061 ccggctgttg atctgaatat aggggctttc aaccatcatc ttcactgttt tgccattttt
     3121 ggttatggaa agggtattaa tagaccccaa ccattttaat tttgccccga tactctgggc
     3181 cagagctttt gaccttacca agattgctcc attgcttttt acgggatcaa gttgggctgt
     3241 tttattttca ccatttatgt atattttggg catatcggct gataatacag cctgactgcc
     3301 caaaattaag attaaaagca agaatattac tgtgtatttt ttcaagttca ttgataacat
     3361 cctccggcct taagattgtt gttaaataaa cattcttact actaatattc aacgttatta
     3421 acttatttcc tgccgttttg attaaaaatg gcaatttata taaaaaaagg actttccgta
     3481 agccctttaa taataatcca ctgtatggtt atataaatag actcccggaa aatcctcaat
     3541 aaaacttatt attttattta tgaccttatc aagatagcgc cggtcactag ttactgtcac
     3601 gagacctaaa gtagcatttt tccagagatc cttattatct acctcggcca ccgccacatt
     3661 gaacttgttt ttacacttat cagtaaggct tttgataata ctccttttgt cttttaaaga
     3721 ctgagccatc ggaatctgta attcaacagt aattactcct acaaacatat tatcaccttc
     3781 ttaagagacc gttaaagaaa acctccttga cacttctatc tatattctca attgggaatt
     3841 cagaagagaa caatgcccag tgagaagaag caacacttaa aagcccgaat aagcctgaac
     3901 caattgtttc tgtatctatg tctttaaata gaccttcttt tttaccctct tttataatat
     3961 cttcaaaaat agttatgtaa tcttctttta attttaaaat ttgatcttct aaaccctctc
     4021 tgtaactcca catttccctt aaataaaata caaaatagtc gtggttatct ccaaagtact
     4081 ggagatgaga atcaatggcc gtctccatct tatctttagg agacattcct tctttaatac
     4141 tatcctttac ataattaatt agctttactg tcccgtagtc taataaaaaa ttcaagagat
     4201 attctttact ttcaaagtaa ttgtaaaagg tgcctttacc aactcccgtt tctcgggcaa
     4261 tttcttcaac agtagtttga ttaaaactct ttttagaaaa caggttaatt gcagctacac
     4321 atatcctact ctttttgtct tcaagatcat caaaaagttc atattttctt gtcataataa
     4381 ccccttctct tttcaaaatt caatttaata gtatcagacc accactacaa tgtcaaagaa
     4441 gtgaggtccc acagataata ttaattttac tatcatttag actaattata ttatataata
     4501 tttttaaaat attttcttat ttgatattat aaagaaaagc caaaggagtc aacttctccc
     4561 acctaatcag aaacaagatt aggaaggaac ttgttaaata acaaactgca ctcagtacag
     4621 tctttaacaa gatttacacc ggctctatct cacaagtggt tgattcaaga aagagccagt
     4681 tgaagggcag cgactaaaaa ccctatccct actgtaaaaa caataggggc tacaggttac
     4741 cctgtatgct atcattggat agctctttta gcaatattga cacttgcatt ataatcagca
     4801 ttttgtagat gatgattg
//
LOCUS       DATGCJ010000207         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_388194, whole genome shotgun sequence.
ACCESSION   DATGCJ010000207 DATGCJ010000000
VERSION     DATGCJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388194"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1844)
                     /locus_tag="VKN64_09390"
     CDS             complement(411..1844)
                     /locus_tag="VKN64_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HMA60493.1"
                     /translation="MSQIIIWLLAYIKLQEKIILYLFVLLTGKNITPKVDTPISKQYQ
                     YLQIDELPIIEVFEKLDHNQLLAEYKTKHGKVLKPIKRRNPKHKVPHNISCPRCQAPH
                     VYIYDNNGGKGQYLCKICDFPFIKGQVYQKSISFKCPHCGKSLEKIKVRKQFIIHKCK
                     NDFCSFYQYNLAVMTKKERKLFAQNPYAFKLHYIYREFTFDFKPLSKESTVIPKVDIT
                     KIHSSHQVLGLVLTYYTNYGLSSRNTAAIMKDIHGVDISHQTVLNYADAVSKYIKPYV
                     DNYPYKLSNSFCGDETYLRVNGKWQYLFFFFDALKKIILSYRISPDRDTEAAVRALDD
                     TLKKIGQLPEDLTFVVDGNPIYRLAQHVFYQHGIHFDLKQVIGLTNNDEVSREYRPLK
                     QIIERLNRTFKREYKTTYGFKSSAGSEAFTILFVAYFNFLRPHSALEDKRVPVIIPEL
                     KNISNMPGKWITLIGLAQEYIEGLQAS"
     gene            2009..2593
                     /locus_tag="VKN64_09395"
     CDS             2009..2593
                     /locus_tag="VKN64_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HMA60494.1"
                     /translation="MILAPYKYTKEVIETEKKILEGEMVEKEVIKEKEFINFRPVYVF
                     DISQTEGEPVPDWKIEVEDSNRKILNPLVSYAKGEDIRVEFKRLREGLKGYSEGGKIV
                     LGERLNNTEKAVTLCHEIAHEKLHNDSRDNDKLTKEIVEMEAEAVSFLVSDYFNIDNP
                     SGKYLALYKKSYDLMESFKRINRTSTEIIDFIKG"
BASE COUNT          906 a          374 c          557 g          793 t
ORIGIN      
        1 ttactgctgg atggagaaga agatagaatg gtaagctttg atggtaaaaa tgtaatagat
       61 gttgatttaa acaaagcatt aactaaagaa aaaaatatag atatggatat atataacttg
      121 gccagaatat tatcattata gggtagtgta aaaagttcta tgaaaaagag ctcaaattag
      181 agggaaaaaa caaattacag gaataatttt cattagatga agtcagcgcc cttgacaaac
      241 accagataaa atggttttta aagtcaacga gggcgacgaa aacagatata aaggcatgac
      301 aaaacagccg accctcgcaa attcaatttt accattttac tggtttgatt tgtaaagggt
      361 tcgcttcgcc gctttaaata ctcgggctca aaaacagata tagaggcaaa ctaagaagct
      421 tgcagccctt caatatattc ctgagctagg ccaatcaagg taatccattt acccggcata
      481 ttagagatat tcttgagttc aggaataata accggtactc ttttatcctc taaagcagaa
      541 tgaggtctaa gaaagttgaa gtaagcaaca aaaagtattg taaaggcttc tgagccagca
      601 gaagatttaa aaccataagt ggttttgtat tcgcgtttaa aggttctgtt taaacgctca
      661 atgatttgtt taagagggcg atattctcta gagacttcat cattattggt aagtccaatg
      721 acttgtttta agtcaaaatg gattccatgt tgataaaaga cgtgctgggc aagacggtag
      781 ataggatttc cgtcaacaac aaaggtaaga tcttctggta gttgtccgat ttttttaaga
      841 gtatcgtcga gagctctaac agcagcttcg gtatctctat cgggtgagat ccgataagag
      901 aggataatct ttttaagtgc atcaaagaag aaaaaaagat actgccattt accattgacg
      961 cgaagataag tttcatctcc acaaaaggaa ttagagagtt tgtaaggata attatcgaca
     1021 tagggtttaa tgtatttact gacggcatca gcatagttta ggaccgtttg atgtgagatg
     1081 tccactccat gaatatcctt catgatagcg gcggtattgc gagaagagag tccgtagtta
     1141 gtatagtaag tgagtactaa accgagaacc tgatgagagg aatgaatttt tgttatatcg
     1201 accttaggta taacagttga ctctttgctt aagggtttaa agtcaaaggt aaattcacga
     1261 tagatataat gcagtttaaa agcataagga ttttgagcaa aaagttttct ttccttttta
     1321 gtcatgacag ctaagttata ctgataaaaa gagcagaagt cgtttttaca cttatgaatg
     1381 ataaactgtt tacgcacttt aattttttcg agtgatttcc cacagtgtgg acatttaaaa
     1441 gatattgatt tttgataaac ttgtccttta ataaaaggga aatcacagat cttacagaga
     1501 tactgacctt tgccaccgtt attatcatag atatagacat gaggagcttg acagcgggga
     1561 caggagatat tatgaggtac tttgtgtttg ggatttctcc ttttaatagg tttaagcact
     1621 ttaccgtgtt tagttttata ttcagctaag agctggttat ggtccaactt ttcgaagact
     1681 tcaatgatag gcagttcatc aatctggaga tactgatatt gcttactgat gggtgtatcg
     1741 actttaggtg taatattctt gccggttaaa aggacaaata aataaagaat aattttttcc
     1801 tgtaatttga tataggcgag cagccagata ataatttgag acataattct tgccaccttt
     1861 ctattttttg ttttattgat agatacattt tacccaaaaa acaagggggt ggcaaggttt
     1921 taaaccaaga tccttgtttt gacagggttt tggactatat tagttagaaa acttttgaca
     1981 atacgtcaaa aaaggagagg agggaataat gatattagca ccttataagt atactaagga
     2041 agtcattgaa acggaaaaaa aaattcttga gggtgagatg gttgaaaagg aagtaattaa
     2101 ggaaaaggag ttcataaact tcaggccggt ctatgtattt gatatttcac agacagaagg
     2161 agagcctgtt cctgattgga aaattgaagt tgaagattct aatcgaaaaa ttttaaatcc
     2221 ccttgtcagt tatgcaaagg gggaagatat tagggttgag ttcaaaaggc tcagagaagg
     2281 cttaaaaggc tactcagagg gaggaaaaat agttctcggg gaaagactta ataatactga
     2341 aaaagcggtt acattgtgcc atgagatagc tcatgagaag cttcataatg acagtcgtga
     2401 caacgataag cttacgaaag aaatagtaga gatggaagct gaggcagttt cctttctcgt
     2461 gtcagattat tttaatattg acaatccctc cgggaaatat ctggctcttt ataagaaaag
     2521 ctatgatcta atggaaagct ttaaacggat taacagaacc agtacggaga tcattgattt
     2581 tatcaaggga taaataattt atcataaatt tttgaatgta attaataata
//
LOCUS       DATGCJ010000208        10849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_297737, whole genome shotgun sequence.
ACCESSION   DATGCJ010000208 DATGCJ010000000
VERSION     DATGCJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297737"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VKN64_09400"
     CDS             <1..823
                     /locus_tag="VKN64_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMA60495.1"
                     /translation="IDVYDEIDNYYNNSDEYVKYCSHLPEEELIDDTLLPELKNQKET
                     YPSLRMTHCLETLNRKKIVNHKYHKSTVELFDDFNLLDKNTVLFHCTHVNNDDIELIA
                     DRQASIVYCPVSNYWSGAGYAPVEKFLQEDINILLGTDFLLTDLWEVMRNAYYYLKTH
                     TEPERYTAKDIFKMVTVNAHRVIAPEMKIGKIDIDYKADLQFINKNDTQIWPLLNKNG
                     FSNILHNLLMHGNASMVENLMIDGKWVLKDNKIIPFDENFILIRYRHILQKLYGF"
     gene            851..1129
                     /locus_tag="VKN64_09405"
     CDS             851..1129
                     /locus_tag="VKN64_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60496.1"
                     /translation="MTDFKQILVLDNEIEANIMEDILKDRDIPHIIQSYHVPGYDGVF
                     QFHMGWGHIESAEKYREEIIEIYNDIKEEAAKKARENFEEEGGKNEKD"
     gene            1116..2189
                     /gene="corA"
                     /locus_tag="VKN64_09410"
     CDS             1116..2189
                     /gene="corA"
                     /locus_tag="VKN64_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMA60497.1"
                     /translation="MKKIRKISEKRARKAGQPPGVLKYLEEEPQEEIKISYIDYDKDS
                     YQEEEITEISELKKLKESETVSWLNIIGLSDVEKIEKLCNVFNIHPLVQEDIIHVHQR
                     PKMEEHKDSLYIVLKMLNYNESEEDIEVEQVSIVVGDNYVITFQERAGDVFDPIRKRL
                     KNEKGRIRTMQADYLAYTLIDVIVDNYFIILEKIGDKIEIIEEELVNQPSDDTLQKIY
                     KYKNEMIFLRKSVWPLREVINGLEKSDTKFINSSVKLYLRDVYDHTIQVIETVETIRD
                     MLGGMLDIYLSSISNKMNEVMKVLTIIATIFIPLTFIAGIYGMNFEYIPELTWQNGYF
                     VVLIVMAVIAAYMVYYFKKKKWL"
     gene            2397..3461
                     /locus_tag="VKN64_09415"
     CDS             2397..3461
                     /locus_tag="VKN64_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMA60498.1"
                     /translation="MKKRLTITFLVLITVMLVTPSVLADSMEINLEKAYEMTLQDNTS
                     LKIAQKDLENKEVQYEKSKAQNLLNQSNYSELQAEYNLIAAKKSYIDTANNLLKSTLQ
                     QYTNILLKKQNIAVLDKQIKLNENLLSEVKAQYEVGEKSQLDILDQQIELNDLIQSRE
                     QAKNEYEQLKTEFKVQLGIDRGTEITLVELTEPKYLNLSEEEIYNQALNNSWELRMNN
                     LNLELAEIDLKKKEVVSTSEMDKSISENNVDIAELQIEKQRESLSNQARDFNNQLNNI
                     KSNIMISKDKIKKANETYNILEEQYKSGLITQNDLSEGEISVLQSEYQLYNAYMSYYI
                     QKLNVNKFMNPGAGVLADEN"
     gene            3451..4845
                     /locus_tag="VKN64_09420"
     CDS             3451..4845
                     /locus_tag="VKN64_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMA60499.1"
                     /translation="MRIKNFKIVLIISIFVLLFSSLTIQAAEYNLKELIKTGLNNNIE
                     IQKLENELETLQRNMKLTKARSDWQANLSLNKELIEDDSITVTRSNNEQVNLSLNKNF
                     AQDRVTVSPNAYYNFDGSEVIYGLNMNIDLYPNLPSESFKTLLNLNNQLNQKQKELFN
                     KQAELAKSWIDKYLQLVRLDENIDVLEERLSITEDSYQEIQKQIEIKEAGQQDLLQAE
                     IDLNEAEYNLKQSQQNFKRVKMQLLADLQLEEDTEIVLDDNNTVLAELKGLTADLNID
                     DLDQEKMVENVVKTSSQFATILNNKNYLEKELEWLKQEDAPQVTLEGSYDSETDFTAS
                     VNVSYNIFDSGVQAMTVENKKQEIENSELSLEQLYQQTANNIDNLIDQVKLAQLEKET
                     SQLKYDKAVKDTKIVKQQFAAGAVEEEVLTNNQLNERNTLINLNSSNDQVFLNKLDLL
                     TLTRPAEIVEEVKK"
     gene            4845..5876
                     /locus_tag="VKN64_09425"
     CDS             4845..5876
                     /locus_tag="VKN64_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMA60500.1"
                     /translation="MKKALIITAVIVLVLAGGYFGYTRYYAQNNKQEPLQITPKNTMK
                     VETGRMKKTISASGNIYPIEEQNLTFSTDGTVAAVNVEEGNNVEEGEVLIELKNNQAQ
                     LQFLKAENQLKNARINGSPNAVREAELDYEIAKERLDDTKLEAPYSGVITELLVQKGD
                     YINSGQEVATLIDNTQYEVKANIDESELANLEVGQNVEISMEALPGLQLTGQLTEIGS
                     KAESTSGVVTIPITVLINSVHDSFKPGLSADMEIIVGQVQDKLIVPVTAILNKEGKKY
                     VQKVVGENTQEVEINTGLTDGMRVVIETGLESGDEIIVNSAAMAQPGGSGQNQSGMFR
                     GGPPTGGGN"
     gene            5879..6556
                     /locus_tag="VKN64_09430"
     CDS             5879..6556
                     /locus_tag="VKN64_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60501.1"
                     /translation="MIRLEDVTKVYKNGKVEVQALKNVSFEIEEGEMVAIMGPSGSGK
                     STLMHIIGCLHKATSGFYSLNGQDISKASENELARIRNKEIGFVFQQFNLLPKHSVLH
                     NVEVPLIYKGESKKERIERAKDLLNKVGLGHRLKHYPNEISGGQKQRVALARALANNP
                     SIILADEPTGNLDTKTGEEIMDLLQRLNEQGHTIILVTHESNIAAYARRTINLVDGRV
                     KEDVKAS"
     gene            6553..7764
                     /locus_tag="VKN64_09435"
     CDS             6553..7764
                     /locus_tag="VKN64_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60502.1"
                     /translation="MIFETIKIAIQSLFSNKLRTLLSMLGIIIGVGAVIAVVSIASGS
                     QAQITSNISALGSNLINISPGISRGGPGSISRSSTDLFTLELGDNIEKNSPHVKRVIP
                     NDSRSGLFIKGDNNYRASVVGTEDYYQVINKYYPQSGMFFSDYDVQNSTNVIVLGSNL
                     VNELFPDSNPVGQNLKFNINNRTQIYKVIGVMEEKGRGFIGDLNGQAYIPISTFMNKV
                     SNSHYVSNFVAQAESSETADKALQEIEYLLDSYIAEDENYNIFSQDQLLNTINDVTNT
                     MRLMLSGIAGISLLVGGIGIMNIMLVSVTERTKEIGIRKALGAKKRDILFQFIIESLT
                     LSSIGGIIGILFGGAAAYFIAQAGGWPFIMSMSIIALAFSFSLVVGMFFGIYPAYRAS
                     KLDPVDALSYE"
     gene            7797..8483
                     /locus_tag="VKN64_09440"
     CDS             7797..8483
                     /locus_tag="VKN64_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA60503.1"
                     /translation="MSYNVYLVEDDKSLNSILTSYLKNEGWNVKSFHNGLAAVEVIDE
                     KPDIWVLDIMLPDIDGFELIDKIKANDPEIPVIFISARDEDLDRITGLEKGSDDYIAK
                     PFSPRELVIRIKKIFQRIYGDKQGKEIIEYGDYIIDLQSRVVKIDGEQKEVVDLTSRE
                     FDLLVFFLNNISHALTREQILNNVWGRDYFGSDRVVDDLIRRLRKKLPDLKIETIYGH
                     GYRLVRKDEV"
     gene            8473..9840
                     /locus_tag="VKN64_09445"
     CDS             8473..9840
                     /locus_tag="VKN64_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMA60504.1"
                     /translation="MKFNFKDKPLSFQIWIILGLILGGTAIILSIIIPVILRSSFTRE
                     TFARLEDAQEYLLTYGNLQDELFEYFPVFPQREEFRSPSFRIVRHTLITPEGMMIGGT
                     TPVQYGQVFVQDALDQASDTSNYQEQLEDRVLFYRINKIQLNDHPYFLVSYITGTYRD
                     NLVEAIFNQILKLLLVISVVTWLASLLLARYLTRPLKRLENKVKNIANKKWDVEVDLE
                     REDEIGRLGKTIDWMRQQLIERDEKQQEFLQEASHELKTPIMVIRSYIQSLMDGIIPK
                     EDMDDTLQNIEDETFKMEERVRSLLNITKMDHLSNQTLKVEDVNLKELVLKKVNRFEW
                     RNVDIDWELNLKEISCLCDREKLEVVLDNIFDNQLKYAANKVTVDLYEEDGNNMIRVF
                     NDGPPIKEENKDKIFQKFQKGANGEFGLGLAISKFILDLHDGEIWVENEEGGAAFYIK
                     IPPQK"
     gene            complement(9909..10391)
                     /locus_tag="VKN64_09450"
     CDS             complement(9909..10391)
                     /locus_tag="VKN64_09450"
                     /inference="COORDINATES: protein motif:HMM:NF042802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HMA60505.1"
                     /translation="MIKHIFKNINNDERIKRLIYKYGFESFAIMSLLLSGIIIYRNLL
                     NGISLSEYKIEFLVLAAGGIYFIMRTAFGGVISLPDNQKERRSFIKYIVLGNVIFGVL
                     FGTFISIRNTILYLDGTYNMLSFSILIITSVSAMLFGSAITGVFLVLSNYYANKDLAE
                     "
     gene            complement(10384..10590)
                     /locus_tag="VKN64_09455"
     CDS             complement(10384..10590)
                     /locus_tag="VKN64_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60506.1"
                     /translation="MKNLKLKAARAQKDLTQKELADIVGVTRQTIGLIETGKYNPTLS
                     LCIDICKTLNKTLDELFWEDDKND"
BASE COUNT         3972 a         1681 c         2107 g         3089 t
ORIGIN      
        1 tattgatgta tatgatgaaa tagataatta ttataataac tcagatgaat acgttaaata
       61 ctgttctcac ctgccggaag aagaactaat tgatgacacc ttattacccg aactaaaaaa
      121 tcaaaaagaa acctatccct cgttaagaat gacccactgt ttggaaactc ttaatcgaaa
      181 aaagattgtt aatcataagt atcacaaatc aactgtagaa ttatttgatg attttaattt
      241 actggataaa aatactgtac tcttccactg tacccatgtc aataatgatg atatcgaatt
      301 aatagctgat cgtcaggcct cgatagtcta ctgtcccgtt tctaactatt ggagtggggc
      361 cggttatgcg cccgtagaaa agttcctaca ggaagatatt aatattttac tgggcaccga
      421 tttcttgtta actgatctct gggaagtaat gagaaacgcc tattattacc ttaaaactca
      481 cactgaaccc gagagatata cggccaaaga tatattcaaa atggtgactg taaatgccca
      541 tcgggtaata gcgccggaga tgaaaatagg taaaatagat attgattata aagctgacct
      601 gcagtttatc aataagaatg atacccagat ttggcctctt ttaaataaaa atggattttc
      661 aaatatttta cataacctgc taatgcacgg taatgcaagc atggttgaaa atctaatgat
      721 tgacggcaag tgggtgctca aagataataa aattatacct ttcgatgaga actttatact
      781 tatcagatac agacatatcc tgcaaaaact gtatggtttt tagaccatta atatagagga
      841 ggaagcgaaa ttgactgatt ttaagcagat attagttttg gacaatgaga tcgaagcaaa
      901 tattatggaa gatatattaa aagaccgtga tataccccac attatccaat cctatcatgt
      961 acctggatat gatggggttt ttcaatttca tatgggatgg ggtcacattg agtcagccga
     1021 aaaatacagg gaagaaatta ttgaaatcta taatgacata aaggaagagg cggcgaaaaa
     1081 ggcgcgcgaa aattttgaag aagaaggagg taaaaatgaa aaagattaga aagatctctg
     1141 aaaaaagggc ccggaaagca ggtcaaccac caggtgtttt aaaatattta gaggaagagc
     1201 cacaagaaga aataaagatt tcttatatag attatgataa agacagttac caggaagaag
     1261 agataacaga gatatctgaa ttaaaaaagt taaaggagag tgagacagtc tcctggctta
     1321 atattattgg cctgtccgat gtggaaaaaa ttgaaaaatt atgtaatgta tttaatatac
     1381 atcccctagt ccaagaggat ataatacatg tacaccagcg accaaagatg gaagaacaca
     1441 aggattcatt atatattgta ttaaagatgt taaactataa tgaaagtgaa gaagatattg
     1501 aggtagaaca ggtaagtata gtcgttggcg ataattatgt aatcactttc caggagaggg
     1561 caggtgatgt attcgacccg attagaaaaa gattaaaaaa tgaaaagggc aggatcagaa
     1621 cgatgcaggc tgattatctt gcctatactt tgatagatgt gatagttgat aactatttca
     1681 taattttaga aaagattggg gataaaatag agattattga agaggagctt gtcaatcaac
     1741 cttccgatga taccttacaa aagatatata aatataaaaa cgagatgatt tttttacgta
     1801 aatcggtctg gccgttaaga gaagtaatta acggcttaga aaaaagtgat acgaagttta
     1861 taaactcttc tgtgaaactc tatctcagag atgtatatga ccataccata caggtaattg
     1921 aaacagtgga aacaatacgg gatatgctgg gtggaatgct cgatatttac ctatcgagta
     1981 tcagcaataa gatgaacgaa gtaatgaagg tattaacaat tattgccacc atatttattc
     2041 cacttacttt tatcgccggt atatatggaa tgaactttga gtatataccg gaattaacct
     2101 ggcagaacgg ctattttgta gtcttaattg tgatggctgt gattgctgcc tatatggtat
     2161 attatttcaa aaagaaaaaa tggctttgat tatatccccc aaaccataat cgtgtttggg
     2221 gcttattatt ttaaaaagtt aatataatct taatatttaa ataagtaaaa ctaaagtttt
     2281 ttccaatata ataaataaat agcattatca cataatttca cataatcatg tgataattat
     2341 ttgataattt ttaaataaaa ttaaaattaa gagaataggt aggaggtata ttcaccatga
     2401 aaaagagatt gaccattact tttttagtat taattacagt tatgttagtt actccttcag
     2461 tattggcgga cagtatggag attaatttag agaaggccta tgaaatgaca ttacaggaca
     2521 atacttctct aaaaatagct cagaaagatt tagaaaacaa agaggttcaa tatgaaaaaa
     2581 gcaaagcaca aaacttattg aaccagtcca actacagtga acttcaggca gaatacaatc
     2641 ttatagctgc caaaaaaagt tatatagata ctgctaacaa tttattaaaa tcaacccttc
     2701 agcagtatac aaatatccta ttaaaaaagc agaatatagc tgttttagac aaacagatta
     2761 aattaaatga aaatttatta agtgaggtca aagcccagta tgaagtaggt gaaaaaagcc
     2821 aacttgatat cttagatcaa cagattgagc ttaatgacct tattcagtcc agagaacagg
     2881 ccaaaaatga atatgaacag ttaaaaactg aatttaaagt tcagctcggc atagatagag
     2941 gtaccgaaat taccctggtt gaattaacgg agccaaagta tttaaattta agtgaagagg
     3001 aaatatataa tcaggcttta aataacagct gggaacttcg gatgaacaac ttaaatttag
     3061 aactggctga aatagactta aagaaaaaag aagtagtatc cacatcagaa atggataaaa
     3121 gtatatctga aaataatgta gatattgctg aactacagat agaaaaacaa cgagagagtc
     3181 ttagcaacca ggcccgcgat ttcaataacc agcttaataa tattaaaagt aatatcatga
     3241 tctcaaaaga taaaataaaa aaggccaatg agacctataa tattttagag gaacagtaca
     3301 aatcaggtct aataacccaa aatgacttaa gtgaaggcga aatttcggtt cttcagtctg
     3361 aataccagct ctataacgct tacatgagct actatattca aaaattaaat gtaaataagt
     3421 tcatgaaccc aggagccggg gtgttagcag atgagaatta aaaacttcaa aatagtcctg
     3481 ataattagta tatttgtact acttttttcc tcgctcacta tccaggcagc cgaatataat
     3541 ctaaaagaac tcatcaaaac aggcctaaat aataatatag agatacaaaa actggaaaac
     3601 gaactagaaa ctctgcaaag aaatatgaaa ctgaccaaag cccgcagtga ctggcaggct
     3661 aatttaagtt tgaataaaga attaatagaa gatgattcaa tcaccgttac aagaagcaat
     3721 aatgaacagg taaatctttc actcaacaaa aactttgccc aggatagagt aacggttagc
     3781 cccaatgcat actataattt tgacggttca gaagtgatct atggcttaaa catgaatata
     3841 gatctttatc ctaatctacc ttccgaaagc tttaaaaccc tgctcaattt aaataaccag
     3901 cttaatcaaa aacaaaaaga gctgtttaac aaacaggccg agttagcaaa aagctggata
     3961 gacaagtatt tacagcttgt tagattagat gagaatattg atgtattaga agaaaggtta
     4021 tcgatcactg aagatagcta ccaagaaatt caaaaacaga ttgaaatcaa agaagccggg
     4081 cagcaggatt tactgcaggc tgaaattgac ttaaatgagg ctgaatataa tttaaaacag
     4141 agccaacaaa acttcaaaag ggttaaaatg cagctgcttg ccgacctgca gttagaagaa
     4201 gatactgaaa ttgtcttgga tgataataat actgtcttgg ctgaacttaa aggattaaca
     4261 gctgatttaa atatagatga tttagatcaa gaaaaaatgg ttgaaaatgt agtcaaaacc
     4321 agcagtcagt ttgccactat tttaaataac aaaaattatc ttgaaaaaga acttgagtgg
     4381 cttaaacaag aagatgcccc ccaggtaact ctagagggta gttatgacag tgaaactgac
     4441 tttactgctt cggtaaatgt aagctacaat atcttcgaca gtggagtcca ggcaatgacc
     4501 gtggaaaaca aaaaacagga aattgaaaat agtgaattat ccttagaaca gctttatcaa
     4561 cagactgcca ataatataga taatttaatt gaccaggtaa aattagctca attagaaaaa
     4621 gaaaccagcc aattaaaata tgataaggca gttaaggata ctaagatagt aaaacagcag
     4681 tttgctgctg gtgccgtaga agaagaggtt ttgacaaaca atcaacttaa tgaaagaaac
     4741 actttaatta acttaaatag ttctaatgat caagtttttc taaataaatt agacctctta
     4801 accttaacaa gaccggctga aatcgtggag gaggttaaaa agtaatgaaa aaggctttaa
     4861 ttattacagc agttatagta ctagtattag cgggaggata cttcggctat actagatatt
     4921 atgcacaaaa taataaacaa gaacctctac agattacgcc taaaaatacg atgaaagttg
     4981 aaaccggccg catgaaaaaa actatttcag ccagtggtaa catttaccct atcgaggaac
     5041 aaaatttgac cttttcgacc gatggtactg tagcagccgt aaatgtagaa gaaggtaata
     5101 atgttgaaga aggtgaagtt ttaattgaat taaaaaataa ccaagctcag cttcaatttt
     5161 taaaagcaga gaaccagtta aaaaatgcca ggattaatgg ttctcccaat gcagtgagag
     5221 aagctgaatt ggattacgaa atagctaaag aaagattaga tgataccaag ttagaggccc
     5281 cctattctgg tgttataact gaactgttag tgcaaaaagg agattatata aatagcggtc
     5341 aagaagtggc aaccttaata gataatactc aatatgaagt gaaagccaat attgatgaaa
     5401 gtgaactggc taatcttgag gttggacaaa atgttgaaat tagcatggag gcattacccg
     5461 ggttacagct aacagggcag ttaacagaaa tcggatctaa agccgagagc accagcgggg
     5521 ttgttacaat ccccattact gttttaataa attcagtcca tgactccttt aaacccgggt
     5581 tatctgctga catggaaata atagtgggac aggtccagga caaattaatt gtacctgtga
     5641 cagctatttt aaataaagaa ggcaagaaat atgtacaaaa agttgtcggt gaaaataccc
     5701 aggaggtaga aataaatacc ggtttaacag atggaatgcg agtcgtaata gaaacgggct
     5761 tagagagtgg agatgagatt atagtaaata gtgcagcaat ggctcaaccg ggaggttcag
     5821 gccagaacca gtccggcatg ttccggggag gcccacctac cggtggtggt aattaattat
     5881 gattagatta gaagatgtaa caaaggtata taaaaatggt aaagttgaag tccaggcctt
     5941 aaaaaatgta tccttcgaaa ttgaagaggg tgagatggtt gctatcatgg ggccctccgg
     6001 ttcaggtaaa tctactttga tgcatattat tggctgtctg cataaagcaa cttccggttt
     6061 ctatagtctt aatggacagg atatatcgaa agccagtgaa aatgaattag cccgtatcag
     6121 gaataaagaa attggatttg tctttcaaca gtttaacctt ttacccaaac attctgttct
     6181 tcataatgtg gaagtccctt tgatatacaa aggtgaaagt aaaaaagaaa gaattgaacg
     6241 ggccaaagat ctattaaata aggtgggtct tggccacagg ttaaaacact atcccaatga
     6301 aatctcgggc ggacaaaagc agagagtggc gcttgcgcga gcattggcca ataacccctc
     6361 aattatttta gccgatgaac caactggaaa tctcgatact aaaaccgggg aagagatcat
     6421 ggatctcttg cagcggttaa atgaacaggg ccataccatc atcttagtta cgcatgaatc
     6481 caatatagct gcctatgccc gtcgtacaat aaatctggta gacggtcggg ttaaagagga
     6541 tgtgaaagca tcatgatctt tgaaactatt aagattgcaa ttcagagcct tttttccaat
     6601 aaattaagga ctttgctttc tatgttggga atcataattg gagttggggc tgtaatagcg
     6661 gttgtctcga tagcttctgg ttctcaggcc caaatcacct ctaatatttc tgcccttggt
     6721 tctaacttaa taaatattag tccaggtatt agtagagggg ggccaggtag tattagtaga
     6781 tcctctacag atctttttac gttagaatta ggagataata ttgaaaaaaa tagtccacat
     6841 gtcaaaagag taattcccaa tgacagccgc agcggacttt ttatcaaggg agataacaat
     6901 tatcgggcta gtgtagtagg aacggaagat tattatcagg tcatcaataa atattatccc
     6961 cagtctggca tgtttttttc tgattacgat gtccaaaaca gtactaatgt aattgttttg
     7021 ggttctaatt tagtaaatga actgtttcca gactcaaatc ccgtcggaca aaatttaaag
     7081 tttaatatca ataataggac tcagatctat aaagttatcg gtgttatgga agaaaaaggt
     7141 aggggattta taggagatct gaatggacaa gcctatatcc cgatttctac atttatgaat
     7201 aaagttagta atagccatta tgtaagtaac tttgtagccc aggctgaatc cagtgaaact
     7261 gccgataaag ctttacagga aattgagtac cttttagata gttatattgc tgaagatgaa
     7321 aactataata tctttagtca ggaccaactt ttaaatacaa ttaatgatgt aaccaatacg
     7381 atgagattaa tgttaagcgg aattgccggt atttcattat tagtcggtgg tatcggtata
     7441 atgaatataa tgcttgtttc agtaaccgaa aggaccaagg aaattgggat ccgaaaggcg
     7501 ctgggagcga aaaaaagaga tatattattc cagtttataa ttgaatccct taccctaagc
     7561 agtattggcg gtataattgg tatcctgttc ggtggtgcag ctgcctattt tattgcccag
     7621 gccggcggct ggccttttat aatgtcaatg agtattatag ctttagcctt ttcattttct
     7681 ttagtggtag gaatgttctt tggaatctat cccgcttacc gggcttctaa attagacccg
     7741 gtcgatgctt taagttatga ataaattagt gtaaaatata ctatagaggt gaaaatatgt
     7801 cctataatgt atatctcgta gaagatgata aaagtttaaa ttcaatactg acctcttatc
     7861 taaaaaatga gggatggaat gtcaaatcat ttcataacgg tcttgcagct gttgaagtaa
     7921 tagatgaaaa acccgatatc tgggtattag atatcatgtt accggatatt gatggttttg
     7981 aactaataga taagattaaa gcaaatgatc ctgaaattcc tgtaatcttc atctcagccc
     8041 gggatgagga tctagaccgg attaccggct tagaaaaagg tagtgatgac tatatcgcta
     8101 aacctttttc tccccgcgag ttagttatca ggataaaaaa gatctttcaa aggatttatg
     8161 gagataaaca aggtaaagaa attattgaat acggggatta tataatagat ctacagagcc
     8221 gggtagtaaa aatagatggg gaacaaaaag aagtagttga cttaacttcc cgcgaatttg
     8281 atttattagt cttttttctc aataatattt cgcatgccct cacccgtgaa cagatcttaa
     8341 ataatgtctg gggcagagac tacttcggtt cagatcgagt tgttgatgat ttaattagaa
     8401 ggttaaggaa gaaactaccg gacctcaaga tagaaaccat ttacggtcac ggctataggt
     8461 tggtgcgaaa agatgaagtt taactttaaa gataaacctc tttcatttca aatttggata
     8521 atcctgggac taattttggg cggaactgcc atcattttat ctataataat acctgtgatt
     8581 ttaaggtctt cctttacgag agagaccttt gcccgcctgg aggatgctca ggagtaccta
     8641 ttaacctatg ggaatttgca ggatgaatta tttgaatact ttcctgtttt tccgcagaga
     8701 gaagagtttc gcagtccttc ttttagaatt gtaaggcaca ccttaataac accagaagga
     8761 atgatgatcg gaggtacgac acctgttcag tatggacagg tatttgtaca agatgccctt
     8821 gatcaggcct ctgatacgag taattatcag gaacagttag aagatagggt gcttttttac
     8881 cggattaata aaatacaatt aaatgatcat ccctactttt tagtttccta tattactggt
     8941 acttatcggg ataacttagt cgaagctata tttaatcaaa tcctcaaact attattagtt
     9001 atttcagttg tcacctggtt ggcatcactc ttgctagccc gttatctgac ccgacccttg
     9061 aaaaggttag aaaataaggt taaaaatatt gccaacaaaa aatgggatgt agaagtagat
     9121 ttagagagag aagatgaaat aggaaggtta ggcaaaacaa tcgattggat gcgccagcag
     9181 ttaattgaaa gagatgaaaa acagcaggaa tttctacagg aagcttcaca tgaactaaaa
     9241 accccgatca tggtcatcag aagctatatc cagtctctaa tggacggtat aattcctaaa
     9301 gaagatatgg atgacaccct ccagaatata gaagatgaaa catttaaaat ggaagaaaga
     9361 gtccgttctc tgcttaatat cacaaagatg gaccacttat cgaaccagac cttaaaggtt
     9421 gaagatgtca atttaaaaga actagtcctt aaaaaggtaa atagatttga gtggcgtaat
     9481 gttgatattg attgggaatt aaacttaaaa gaaattagtt gtctttgcga tagagaaaaa
     9541 ctggaagttg ttttggataa tatattcgat aaccagttaa aatatgctgc taataaagta
     9601 acagtggatt tatatgaaga agacggcaac aatatgataa gagtatttaa tgacggccca
     9661 ccgattaaag aagaaaataa ggacaagata tttcaaaaat tccaaaaagg tgcaaatggt
     9721 gaatttggtt taggacttgc gatctccaaa ttcatattag atcttcatga tggtgagatc
     9781 tgggttgaaa atgaagaagg tggagcagcc ttttacatta aaattcctcc ccaaaaataa
     9841 aaacgaacca taaataaaca gcaatgacca ccgcgaggtg gtcattttct tttatataat
     9901 cttttatatt attctgctaa atctttgttt gcatagtaat tggaaagtac taaaaatacg
     9961 ccagtgatgg cagagccgaa gagcatcgca ctgacggaag ttataattaa gatggagaag
    10021 cttaacatat tatatgtacc atctagataa aggattgtat tcctaattga gataaaagtg
    10081 ccgaataaga ctccgaaaat cacattgccg agaactatat atttaataaa agatcttctt
    10141 tctttctgat tatcaggtaa agagatgact cccccgaaag ctgttctcat aataaaatat
    10201 attcccccgg ccgccagcac gagaaactct attttatatt cagataggct tataccattc
    10261 aaaagattcc tatagattat aatgcccgaa agcaaaagag acatgatggc aaaggattca
    10321 aagccgtact tatatatcaa tcttttaatt ctttcatcat tattaatgtt tttaaaaata
    10381 tgtttaatca tttttatcat cctcccaaaa tagttcgtct aaagttttat ttaaggtttt
    10441 acagatatca atacataagc tgagagtagg attatattta ccggtttcta ttaatccaat
    10501 ggtctggcgg gtaactccca cgatatctgc taactctttt tgagttaaat ctttttgtgc
    10561 acgggcagct tttaatttta aatttttcat gcaatcttcg atatctcctt tcaaaaagta
    10621 ttaaatctta tactatcata atctatatat tacaaattgt caagtatata ttgcataaag
    10681 ttataaaaaa gttgcaaaat acaaaaaaaa ctaaaaatat aaaggaatta acagatataa
    10741 aaagaattgt aatatgagca taaaaagaaa gtaggtgata agatgtggaa tcgcgctaat
    10801 ctaaaagcta aagcaaaagg aattttaaaa gggaactatt ggccggcct
//
LOCUS       DATGCJ010000209         6386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_655858, whole genome shotgun sequence.
ACCESSION   DATGCJ010000209 DATGCJ010000000
VERSION     DATGCJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655858"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1059
                     /gene="pyrF"
                     /locus_tag="VKN64_09460"
     CDS             148..1059
                     /gene="pyrF"
                     /locus_tag="VKN64_09460"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMA60507.1"
                     /translation="MIKLKYFIDKLVNNIKKADSRICVGLDPHLDLIPEFLLDNARKE
                     YSDQKEAAAKAVLKFNKKIIDEVVEHTAVVKPQIAFYEKMGLKGLKTLNKTVEYAKTK
                     GLLVLLDAKRNDIGSTADAYAEAYLDNENIDAVTINPYLGFDGVKPFLKYKDKGAFAL
                     IKTSNQSAGDIQDIESKKGKKIYEHMGQLVKNWGKEYLGKSKYSNLGAVVGATYPQEL
                     NQLRSLMPGVFFLIPGYGAQGGGAEDVAAGFDKDGLGAIVNSARGIIFAYRKNDKYSE
                     KEFAIAAGVAAKIMKEKVNKAIEKFHS"
     gene            1077..1841
                     /locus_tag="VKN64_09465"
     CDS             1077..1841
                     /locus_tag="VKN64_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HMA60508.1"
                     /translation="MLVKSNEKVGPGIYKMVFKTEKNPTGKPGQFIHLKINNASYDPL
                     LRRPFSIFDINKKEKSLTIIYKICGRGTRLMKDIKKGDEVDILENLGNGFNLNNKNKK
                     IVLIGGGMGIAPLYLLTKKLYKKNKLMVLLGAKNKEELDFMNNKFSKLDVKVYKATID
                     NSLGFKGTVVDLLKELIKGKQEIDYMYGCGPTAMLRELKEIAINKNIKAEVSLEERMG
                     CGVGVCLSCTVKTVNGNMRACKEGPVFPLKEVIFSE"
     gene            1834..2751
                     /locus_tag="VKN64_09470"
     CDS             1834..2751
                     /locus_tag="VKN64_09470"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HMA60509.1"
                     /translation="MNKEKLNIKLANLQLKNPVITASGTFGFGTELNEYLSVKSLGAV
                     TLKGLTVEESKGNALPRIAETPAGILNSIGLENPGIKRFKEKIIPTIENLKVPIIANI
                     SGYSINEFVQLAANLEKEESINAIEVNVSCPNIKDGGMVFGTDAELIYKVTQAVKKVY
                     SGPIIVKLSPNVTDIIEMAEAAVEGGADIISLINALLGMAIDINKKEAVLANTFGGLS
                     GPAVKPVALRMVYQIYEAIDIPIIGMGGIMKGEDAVEFLLAGASAVGVGTATLRDPTA
                     SIKIISEIDEYLTENKIDNVSEIIGKAHN"
     gene            2766..3344
                     /gene="pyrE"
                     /locus_tag="VKN64_09475"
     CDS             2766..3344
                     /gene="pyrE"
                     /locus_tag="VKN64_09475"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMA60510.1"
                     /translation="MKERALIELFKETNVIQTGHFELTSGRHTDTYLQCAQILQYPEH
                     TDRLAVEIVNLWEKEEIDLVVGPAIGGIIISYAVAAQMGVRNIFSERKNGEMKFRRNL
                     EVKENEKVLIVEDVITTGGSVREVIDLLEESKADIVGISSLVDRSNGQVEFGYIFKPL
                     VQLKVDSYKEEECQLCKKGIPINKPGSKNIKS"
     gene            3746..4678
                     /locus_tag="VKN64_09480"
     CDS             3746..4678
                     /locus_tag="VKN64_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA60511.1"
                     /translation="MRIFGPVPSRRLGRSLGINNVRPKTCSYACIYCQLGRTDYLQLK
                     REEFYKTSEVVKEIEEELDILAEKGEKIDYLTFVADGEPTLDKNLGKTINAIKKFGVK
                     TAVISNASLISDKQVREELKNADWVSLKCDAINEETWRKINRPHGRLDLEKIQQGIKR
                     FSEEYENELVTETMLVEGVNDNEEDLEKNADFIVQLNASKSYISVPTRPPAEDYVKIP
                     PPQKINMAVQIYKDRNIKTEYLIGYEGNKFSSTGDIKKDMLNITAVHPLKEEAVKELL
                     KKCNADWSQIEELIENDQIVEAEYNEAKYYLRNK"
     gene            4812..4976
                     /locus_tag="VKN64_09485"
     CDS             4812..4976
                     /locus_tag="VKN64_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60512.1"
                     /translation="MANNRNRGNQGQRRGMGPQGMCVCPDCGEEVPHERGVPCFEKTC
                     PNCGAKMTRK"
     gene            5497..6234
                     /locus_tag="VKN64_09490"
     CDS             5497..6234
                     /locus_tag="VKN64_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HMA60513.1"
                     /translation="MNIILSPSKTQIHERSREEKGRNILKEDMTEELFDYLKSLSKEE
                     LGEELDVQGNLLDRTYELYQEHSFEDETIPAIECYNGAVFKQLDLDSYDKKQFSYMQD
                     KLIILSPMYGPTHSDTEIWPYRLEMRLKPNGINLYEFWQSVMRDYFADIDLIVNLASN
                     EYSKVVEKNYEGKMIDFYFKEEKEDGSLKTIGYYVKQNRGKLLNELIKNQIKSLDEIK
                     SIELDGYKFDERRSDKQEFRFIKPYNE"
BASE COUNT         2486 a          724 c         1270 g         1906 t
ORIGIN      
        1 taattatctt tgatcctgat aaaaagtggg aagtaaggat tgatgagttt aaatcaaaag
       61 gtaaaaacac cccatttgct ggccataaat taaccgggaa aaatgaaata actttcagtt
      121 cgggtaaatt ggtttatgac aacaggggtg ataaagctga aatactttat tgataagctt
      181 gttaataata taaaaaaggc cgacagcaga atttgtgtcg gccttgatcc tcacctagac
      241 ttaataccag aatttctttt agataatgcc agaaaagaat attcagatca aaaagaagct
      301 gctgctaaag cagtattaaa atttaataaa aaaattatcg atgaagtggt tgagcataca
      361 gcagttgtaa aaccacagat tgcattttat gaaaaaatgg gattaaaagg tctaaaaacc
      421 ctgaataaga cggtagaata tgctaaaaca aaaggtctat tagtactgct tgatgctaaa
      481 agaaatgata ttggatctac agctgatgct tatgctgaag catatttgga taatgaaaat
      541 attgatgcag ttacaattaa tccctattta gggtttgatg gtgtaaaacc atttttaaaa
      601 tataaagata agggggcttt tgcactgatc aaaacctcta atcagagtgc gggtgatatt
      661 caagatattg aaagtaaaaa aggaaagaaa atttatgagc acatgggtca acttgtaaaa
      721 aattggggca aagaatattt gggcaaaagt aaatattcca acttaggggc agttgtggga
      781 gctacctatc cccaagaatt aaatcaactg cgttcgttaa tgccgggagt attcttttta
      841 atacctggtt atggtgctca gggcggagga gcagaggatg ttgcggctgg ttttgataaa
      901 gatggattag gggctattgt aaattctgcc aggggaataa ttttcgcgta taggaagaat
      961 gataaatata gtgaaaaaga atttgcgatt gctgcgggag tagcagccaa aattatgaaa
     1021 gaaaaagtga ataaagcaat tgaaaagttt catagttagg gaaggagaag tttaatttgt
     1081 tagtaaaaag caatgaaaaa gttggacctg gaatttataa aatggttttt aagactgaga
     1141 aaaatcctac aggtaaaccc ggacaattta ttcatttaaa aataaataat gccagttatg
     1201 atcctttatt aagaaggcct tttagcattt ttgatattaa taaaaaagaa aagtcattaa
     1261 ctattattta taagatctgc ggtcgaggta caagattaat gaaagatatt aaaaagggag
     1321 atgaggttga tatattagag aatctaggta atggctttaa tcttaataac aaaaacaaaa
     1381 agattgtatt aattggtggg ggaatgggga tagccccctt atatttactt acaaaaaagt
     1441 tatataaaaa aaataaatta atggtattat taggggcaaa aaataaagaa gaattagatt
     1501 ttatgaataa taagttttcc aaattggatg ttaaagtata taaagctaca atagataaca
     1561 gcctggggtt taaaggaacg gtcgttgatc ttttaaaaga gctaataaaa ggcaaacaag
     1621 aaatagatta tatgtatggt tgtggtccta ctgctatgtt aagggaatta aaagaaattg
     1681 caattaataa gaatatcaaa gcagaggtct ctttagaaga aagaatgggg tgtggggtag
     1741 gggtttgttt atcctgtaca gttaaaacag tcaatggaaa tatgagggct tgtaaagaag
     1801 gaccagtttt tcctttgaaa gaagtgatat ttagtgaata aagaaaagct taatataaaa
     1861 ttagctaatc tacaattaaa aaacccggta attaccgctt ctggcacctt tggctttggt
     1921 actgaattaa atgaatattt atcagtaaaa agtttaggag cagtaacttt aaaggggcta
     1981 acggttgaag agtctaaagg taatgcactt cccagaattg cagagacacc agctggaata
     2041 ttaaactcta taggtttgga aaatcccggt attaaaagat ttaaagaaaa aattattcct
     2101 accatagaaa atttaaaagt acctattatt gctaatatat cgggatattc aattaatgaa
     2161 tttgttcaat tagctgctaa tttggagaag gaagaatcta taaatgcaat tgaagtgaat
     2221 gtttcctgtc caaatatcaa agatggagga atggtttttg gaacagatgc agagctgatt
     2281 tataaggtaa cccaagcagt aaaaaaagtc tattctggcc ctataatagt aaaactaagc
     2341 ccaaatgtaa ctgatatcat agaaatggct gaagctgcgg tagaaggagg ggcagatata
     2401 atttctttaa tcaatgcttt attaggtatg gccatagata ttaataaaaa agaagctgtt
     2461 ttagctaaca cttttggcgg gctttctggt cccgcagtta aaccagttgc cttaagaatg
     2521 gtttatcaaa tttatgaagc tattgatata cctattatcg ggatgggtgg aattatgaag
     2581 ggagaagatg cagttgaatt cttattagcg ggagcatctg ctgtaggagt tggaacagct
     2641 accttaagag atcccactgc ttctattaaa ataatttcag agatagatga atacttaaca
     2701 gaaaacaaaa tagataatgt aagtgaaatt attggaaagg cccataacta aaaggaggta
     2761 taattttgaa ggaaagagca ttaattgaat tatttaaaga aactaatgta atacaaaccg
     2821 gtcattttga attaacttct ggccgacata ctgatactta tttacaatgt gcccagatac
     2881 ttcaatatcc tgaacatacg gatagattag ctgtagaaat agttaattta tgggaaaaag
     2941 aggagataga tttagtagtt ggaccagcta taggaggaat tataatttct tatgcagttg
     3001 ctgcccaaat gggtgttagg aatattttta gtgaaagaaa aaatggagaa atgaaattta
     3061 gaagaaattt agaagtaaaa gaaaatgaaa aagtgttaat tgtggaagat gtaattacaa
     3121 ccggtggttc agtaagagag gtaatagatt tacttgaaga gtcaaaggct gatattgtcg
     3181 ggattagttc tttagtggat aggagtaatg gacaggtaga atttggttat atttttaagc
     3241 ctttagtaca attaaaagtt gattcatata aagaagaaga atgtcagctt tgtaaaaaag
     3301 gaatccctat caataaaccg gggagtaaaa atattaaatc ttaaatgtaa ttttaagcaa
     3361 accaaaataa taatggaagt attatccatt caattttatt taattaccta ctaaattaca
     3421 taattaaata caaaaagttt aaaatgagta ggtgaggaca gatgtcctaa taataaatta
     3481 taaaaaataa gtttttatct taataaatat tccaaattta agaattaata ttctggattt
     3541 gggatatttt tattgccgat cttaaatagt acttatttta ttactcctat attaaatata
     3601 taataaaaat ttgattcaag ttattaggag aagaggattt aactgtaaag ttgttatgaa
     3661 caaatgctaa aaaatttgat attaggagtg aacaagaata taattattat ataatgaaaa
     3721 gagagatata ataggggtgg tagaaatgag gatttttgga ccggtacctt ccagaaggtt
     3781 gggccgtagt ttgggtataa acaatgttag accaaaaacc tgcagttatg catgtatata
     3841 ctgccagtta ggcagaactg attatttaca attaaagaga gaagaatttt acaaaacatc
     3901 cgaagtagta aaagaaatag aagaagaatt agacatcctg gcagaaaagg gtgaaaaaat
     3961 tgattacctt acctttgtgg ctgatggtga accaacttta gataaaaatt taggtaaaac
     4021 cataaatgct ataaaaaagt ttggagtaaa aacagctgtg attagtaacg cctcactaat
     4081 ctcagataaa caggtgaggg aagagctgaa aaacgcggac tgggtttcat taaaatgtga
     4141 tgctattaat gaggagacct ggagaaaaat taatagaccc catggcagat tagatttaga
     4201 aaaaattcaa caagggataa aaagattttc tgaggagtat gagaatgaac ttgtaactga
     4261 aactatgctc gttgagggtg ttaatgataa tgaagaagat ttagaaaaga atgctgattt
     4321 tattgtccag ttgaatgctt ctaagtcata tatatctgtt cctactagac cccctgccga
     4381 agattatgtt aaaataccac cacctcaaaa aattaatatg gcagtacaaa tttacaaaga
     4441 cagaaatata aaaaccgaat atctaatagg ttatgagggc aataagtttt ccagtacagg
     4501 tgatattaaa aaagatatgc ttaatattac agcagtgcat cccttaaaag aagaagccgt
     4561 caaggagtta cttaaaaaat gtaatgccga ctggagccaa atagaagaac ttattgaaaa
     4621 tgatcagatt gtagaggcag aatataatga ggcgaagtat tatttgcgta ataaataatg
     4681 tggtatttag atcattaatc atgttaaaaa ttgagagtta gtatattttt aatcactggg
     4741 ttatttaaat aatcaactaa aataataagg taaaaaaagt taagtctaat ttctaaaagg
     4801 gaggatttct tatggctaac aataggaata gagggaatca aggtcaaaga agaggtatgg
     4861 gaccacaagg catgtgtgtt tgtcctgatt gtggagaaga ggttccccat gaaagaggag
     4921 taccctgttt tgaaaaaaca tgtccaaact gtggggcaaa aatgactagg aaataaaaaa
     4981 attattgtat agtcgggtat ttgttttagc tgatgagata attaaatcaa ctagttttat
     5041 taaattataa aaaagcaagt gtttatgttt ctttactatt cccaaattct agaattagct
     5101 ttctggattt gggatttttt tacgaaaaat aattaagaac gttataaatt aatttattaa
     5161 gtgttttagc taaaagactt aatatatcag aatctgcagt ttcccaacag ttaaaaacac
     5221 taagagaagc agattcagta atgggagaaa ggacaattat gttcattacc tggtcaaaaa
     5281 agaaaatata gagcagataa gtgattatat gattaattta gtagaaagta attagggtga
     5341 ttaaatttat tgctataaag atgatgattg taattttaaa agctaaaagt taaaagtatt
     5401 caatcattta ttaagaggta taatatgtat tatcttgaat aagatatata agaaaataat
     5461 attataatat tatagaaaaa gggaaggatg taaactatga atataatatt gtcacctagt
     5521 aaaactcaga ttcatgaaag aagccgagaa gaaaaaggta gaaacatctt aaaagaagat
     5581 atgacagaag aactatttga ttaccttaaa tcactgtcca aagaagaatt aggagaggag
     5641 cttgatgtac agggtaattt attagatagg acatatgagc tttatcaaga gcatagtttt
     5701 gaagatgaaa caatacctgc tattgaatgt tataatggag cagtctttaa acaacttgat
     5761 ttagatagtt atgataagaa acagttttca tatatgcagg ataaactaat aatactttcg
     5821 cctatgtatg gccctaccca ttcggataca gaaatctggc cttacagact tgaaatgagg
     5881 ttaaaaccca atggaatcaa tctctatgaa ttctggcagt ctgtaatgcg ggattacttt
     5941 gctgatattg atttaattgt aaatcttgct tccaatgaat atagtaaggt agtagaaaaa
     6001 aattatgagg gtaaaatgat cgacttctat tttaaagaag aaaaggaaga cggcagtttg
     6061 aaaacaattg gctattatgt caaacagaac agaggtaaac tgcttaatga actgataaaa
     6121 aaccaaatta aaagtttgga tgagattaaa agcattgaat tggatggata caaatttgat
     6181 gaaaggcgct ctgataagca agaatttaga tttatcaaac catataatga atagaaatta
     6241 gatttaatga ataaaaatag ttcataagtt cagaaatact tgaaagctag aaacctttga
     6301 ttagctttat ctaagtgtcc atatgttatt aagatttaaa tagatatatc ttttaaaagc
     6361 ctgctgaaaa gcaggttttg ttttta
//
LOCUS       DATGCJ010000210         8445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_297939, whole genome shotgun sequence.
ACCESSION   DATGCJ010000210 DATGCJ010000000
VERSION     DATGCJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297939"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /gene="coaBC"
                     /locus_tag="VKN64_09495"
     CDS             complement(<1..780)
                     /gene="coaBC"
                     /locus_tag="VKN64_09495"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMA60514.1"
                     /translation="MEKKTILLGITGGIAAYKMAHVASSLTKMGHEVHVVMTESAQKF
                     ISPVTFRAITGLPVETDLFTEPNKNEVKHIALADKSDLVMVAPATANMIGKIANGIAD
                     DLLSTILTAVTSPVIIAPAMNVNMYNNEIVQDNIQYLEDKGYQLINPSEGMLACGYTG
                     KGRLPEPDILVEYILKTLTKKDLSGKKILVTAGPTREGIDPVRYITNHSSGKMGYAMA
                     KRASCRGAEVKLIAGPNDLEVPLTVDFERINSAEDLYNSVMA"
     gene            complement(785..1360)
                     /gene="gmk"
                     /locus_tag="VKN64_09500"
     CDS             complement(785..1360)
                     /gene="gmk"
                     /locus_tag="VKN64_09500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMA60515.1"
                     /translation="MQRGILFVLSGPSGVGKGTVLDHLMEDYKDINYSVSATTREPRE
                     GEVDGLDYFFIGKEKFNQMKKEDKFLETAQVHGNYYGTPKGYVEDCLKRGDDIILEID
                     IQGAKQIKEKFKDAVYIFLLPPNYEELKNRLEKRDSESEASLKTRLKNASEEISEMEN
                     YDYRIVNDKIQDTVEEVKKVIKKERAKKKGE"
     gene            complement(1381..1641)
                     /locus_tag="VKN64_09505"
     CDS             complement(1381..1641)
                     /locus_tag="VKN64_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HMA60516.1"
                     /translation="MNVNLINIGFGNIVAANRVIAVVSPESAPIKRIIQEARESGMLI
                     DATYGRRTRAVIITDSDHVILSAIQPETVSHRLDDNKEKNEE"
     gene            2095..3834
                     /locus_tag="VKN64_09510"
     CDS             2095..3834
                     /locus_tag="VKN64_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HMA60517.1"
                     /translation="MSIDGILLSAINTNLKDKLQGGRIDKIYQPFKHTLTFTVRNNNQ
                     NYKLLISSDSREARIQITNFSFDNPQYPPDFCMLLRKYLMRGIIKDISQPDFERILKI
                     EVESYNKIYYIFIEIMGRYNNIILTNDNLLVLDAATRLAKDNKRNIYPTSYYEFPPGQ
                     GKLNPLRVDRNTFFEQIPSDFNKKAYQAIMYNFRGIGPASSKEIAYRANMDYNSKYDE
                     LTKEQKDDLLNSFKNIFKKVKSNQYNPSIGIKEGEIDYYSAFTLSYKDEIDKVINFKD
                     TDLLFDYYYENYLKDIELKEKKKKLKGIVNNYLKKNNKKQSEFREDLKKNKNYELFKQ
                     KGELIKANLHQIEKGQKEISLTNYYDSNQKEITIDLDPKKAPIDNAQKYFKKYQKAKK
                     SLKHLKRQIGKLRHEEKYLNQVLFNIDDAINLDELKEIEAELREEGYIKKQKQKNDKR
                     NKEIKPYKYTTEDNYQVLIGRNNKQNDKLTKYIANEGDIWLHTKKIAGSHVIIRNHNN
                     KEVPESTIIEAARLAAYFSKAKESTNVPIDYTPVENVNKPKGAKPGLVYYDNYNTVYV
                     DPAEKEVIEKIEK"
     gene            3902..5659
                     /locus_tag="VKN64_09515"
     CDS             3902..5659
                     /locus_tag="VKN64_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMA60518.1"
                     /translation="MKKLNFDNDVYVIENYDKFKEMKSPKLALALSGGGARGFVNIGV
                     IKALHEAGIYPDIIIGSSMGSIISTLYGSGLNVEDLIKITSTLPYSSLFDINLIPVYS
                     LFQTRKFNYIIEKISPKKDIENFPLNTALLNFDLDSGHKYITSKGRISEILQSTYSIP
                     FYYPINKSKGRYLVDPGLVEMVPAKAAKALSADIVIATTAFDELPYETYKYPMQSLMR
                     MLLLVQKKNSQPILNNYSDIIIQNEVSDFSFKDFDLIEEFIKIGYINAMDKMPEIKKL
                     LKEKNYVSTKPDFYDFDEKQYQKLNTDILFDRIIYPETYFNYLLYYFNNQSFFEQELF
                     RNLVYDFHYGFYYENSHLKFETIFDVNEDRHTELKLRYKKLTENIDFITRLKNNPSYN
                     NNIDYINELKYFIKNSSVSLGWAHFRNDNYYYLNNNIKLERGNFDFENNTDILLNKNK
                     KMQLINSSLLDYNISVNWAFQNKFVYSNTNLLGDPDIYRGQDNEQKEKTSLSSSIIYR
                     LPFSESQEFLYFFSLEELKPSLFIDIESTDQLNAALGLNLNMVSSVMGLKPLNLDLSA
                     AYDKNAQSVIYNFSVDIKF"
     gene            complement(5731..7800)
                     /gene="fusA"
                     /locus_tag="VKN64_09520"
     CDS             complement(5731..7800)
                     /gene="fusA"
                     /locus_tag="VKN64_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMA60519.1"
                     /translation="MSNYKTKDIKNFSFISHGGAGKTTLTEDVLFNAGKIEQIGSVDE
                     GSTKSDFIPEEKDHKYSINSSYFSFKLKEKQVNLIDTPGYADFRGEVASATRMAESSV
                     LLINADSGVEVNTNYVWDIAVENNNSRFVFINKFDKEGTSFKEVFSELEENFEDTLLP
                     ITIPFKEGDEYKGVIDLLSEKFIELTDGEESTSDLNEKQQELLEEYKLELVESIIELD
                     EELMMKYLEDEDISDEEMNNALLKGVLSKEIVPVFSGSALHNSGVNTFMDYLIKLAPS
                     AADRETLKVTGGEDEQEFETNEEGPFIGMVGKTMVDPYIGKLSIFRIYSGRIEKGEEI
                     YLPRTKDNYRVSKLYKLNGSEQDQVEELIAGDIGAVAKLDDLETSDTITKEDLEVKIA
                     PIEFPEPMLIKAADPADEADNEKMSSAIQRYSLEDPTFKVEYNKVTKQLLIKGMGTVH
                     LSVVNDICKRKFDVGFNQVEPKVEYKETIRKKVSTEVKYKKQSGGRGQYGHVMIRFEP
                     LPRGKGFEFDEEIFGGAIPSQYIPAVEKGIVEAKDSGILADYPVVDFKATVYDGSYHD
                     VDSSEMAFKIAASKAFKQAMEKAKPVILEPIMDVTVVVPEDFMGDIMGDLNSRRGKIS
                     GMEPQDGTQRIKAKVPESELFSYATDLKSITGGYGHFTMEFSNYEKVPEDISEKIIER
                     RAKEEDD"
     gene            complement(7931..>8445)
                     /locus_tag="VKN64_09525"
     CDS             complement(7931..>8445)
                     /locus_tag="VKN64_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA60520.1"
                     /translation="DPVEITDELIEDLANSAKLAPSCFNNQPWRYVFVHDEDKLDEVF
                     DTLSSGNQWAHNASMVIGVFAKKEDDCNVKGREYYLFDTGLATSMLMLRARELGLVAH
                     AIAGYSESKAKEVMSIPEDYRLITLVIVGKHSDEIKDILSEKQAKDEKERPPRKDLED
                     FVYHNEFNNI"
BASE COUNT         2819 a         1530 c         1215 g         2881 t
ORIGIN      
        1 agccatcaca gagttatata agtcttctgc gctattgatt ctttcaaaat ctactgttag
       61 cggtacctct aaatcattag ggcccgcaat cagttttact tcagcacccc gacaagaggc
      121 cctcttagcc atcgcataac ccattttccc actggaatgg tttgttatat agcgaactgg
      181 atcaattccc tctctggtag gaccagctgt taccaggatt tttttacctg ataaatcttt
      241 tttagttaag gtttttaaga tatattcaac caaaatatcg ggttccggca aacggccctt
      301 acccgtatat ccacaggcta acatcccctc agaaggattg attaattgat aacctttatc
      361 ctctagatat tggatgttat cctggacaat ctcattatta tacatattaa cgttcatggc
      421 aggggctatg atgaccggtg aagttacagc tgttaaaata gttgagagaa gatcatcagc
      481 tattccattg gctatcttac caatcatatt ggcagtagca ggtgctacca ttaccaggtc
      541 agatttatct gccagggcaa tatgtttaac ctcattttta tttggctctg taaaaaggtc
      601 agtttctacc ggcagcccgg ttatagctct aaaagtaaca ggggaaataa atttctgggc
      661 tgattctgtc atcacaacat gtacttcatg gcccattttt gttaaactgc tggctacatg
      721 tgccatctta taggcggcta taccccccgt aataccgagt aatatagtct ttttttccat
      781 atttttattc ccctttcttt ttggctcttt cctttttaat aacctttttt acctcctcaa
      841 ctgtatcttg aatcttatca tttacaattc gataatcata attttccatt tcgcttatct
      901 cttcactagc gttttttaat cttgtcttta aactcgcctc ggactcggaa tccctttttt
      961 ctaagcggtt cttcagttct tcataattgg gaggtaataa aaatatgtaa acagcatctt
     1021 taaatttttc tttgatctgc ttggccccct ggatatcaat ctctaagatt atatcatcgc
     1081 cccttttcaa acagtcttcc acataacctt ttggggtacc gtaatagtta ccatggacct
     1141 gggctgtttc taaaaattta tcttcctttt tcatctggtt aaatttttct ttaccgataa
     1201 aaaagtaatc tagtccgtcc acctctcctt cgcgaggttc tctggttgtc gctgaaacag
     1261 agtaatttat atctttgtag tcttccatta gatggtctaa aaccgtacct tttccgaccc
     1321 cggagggacc agaaagtaca aacagaattc ctctttgcat atttgatcac ttcctagtat
     1381 ttattcttca tttttttctt tattatcatc taaacgatga gatacagttt cgggctgaat
     1441 tgctgataaa attacatggt cactgtccgt tatgatgaca gctctggttc ttctaccata
     1501 agttgcatcg attaacatac cgctttctct tgcttcctga ataattctct ttataggggc
     1561 tgattcgggg ctaactactg cgatgacgcg gttggcagcc acaatattac caaaaccaat
     1621 atttattaag ttcacattca ttgaagattc ctcctttaca tattttaacg aacacgattt
     1681 actatccgat aattaatatg aaaattgaat ataaaatata aaagaaaatc aaaaatcccc
     1741 ttatttgaat attatgggta tataattgga tatgggggtt tatataagaa aatttctaaa
     1801 tatcttaaag atggtttaaa attatgctta tattaattat ttgtatcctg atcggtttta
     1861 atttttttaa aaaatacaaa ttaataccaa tatattaaga tttttattaa tttatagttt
     1921 tttactaaat tctattttta tcataatatc acagaatctt taatttaaca aggaatttag
     1981 actactccta aacacctgat cctgattgta tattttgact aacatatatt tttaatttat
     2041 actgtaatta tatactaaat aaatgactta ttttgcttca ggaggtaata aaagatgtct
     2101 attgatggaa tattactatc ggcaatcaat acaaacttaa aggataaatt acaagggggg
     2161 agaattgata agatatatca accctttaaa cataccctta catttacagt tagaaataat
     2221 aatcaaaact ataaactact tatcagctca gactcacggg aagccagaat ccagattaca
     2281 aatttttctt ttgataaccc ccagtatccg cctgattttt gtatgctttt aagaaaatat
     2341 ttgatgcgcg gtataattaa agatatttct caacctgatt tcgagcgtat cttgaaaatt
     2401 gaagtagaaa gctataataa aatatattat atctttatag agattatggg tagatataat
     2461 aacataatat taacaaatga taatcttttg gtcttagatg cagccacccg acttgctaaa
     2521 gataataaaa gaaatattta tcccaccagt tattatgaat ttccacccgg ccaaggcaaa
     2581 ttaaacccac ttagagtaga taggaatact ttcttcgaac agataccctc tgatttcaat
     2641 aagaaagcct atcaagctat tatgtataat tttagaggta taggtcctgc ttcaagcaaa
     2701 gaaatcgctt atcgggcaaa tatggattat aatagcaaat atgatgagtt aacaaaagag
     2761 caaaaggatg atcttcttaa tagttttaaa aatatattca agaaagtaaa atccaatcaa
     2821 tataatccgt ctataggaat aaaggaaggt gaaattgatt actattctgc ctttacctta
     2881 tcttacaaag atgagataga taaagtaatt aattttaagg ataccgatct acttttcgat
     2941 tactattatg aaaattattt aaaagatatt gaactgaaag aaaaaaagaa aaagcttaag
     3001 ggaatcgtca ataattattt gaaaaaaaat aataaaaaac agtctgaatt tagggaagat
     3061 ctcaaaaaaa ataaaaatta tgaactattt aaacagaaag gggaacttat caaagccaat
     3121 ttacatcaaa ttgaaaaagg tcaaaaggaa atctccttaa ctaactatta cgactccaac
     3181 caaaaggaaa ttactataga cctagatccc aaaaaagcac ccattgacaa tgcccaaaaa
     3241 tactttaaaa aatatcagaa ggctaagaaa agtttaaaac atttaaaaag acagatcggt
     3301 aagttaagac atgaagaaaa atacttaaac caggttctct ttaatatcga tgatgccatc
     3361 aatttagatg aactaaaaga aatcgaggct gaattacgag aagaaggata tattaagaaa
     3421 caaaaacaaa agaatgataa aagaaataaa gaaatcaagc cctataaata cactaccgaa
     3481 gataattatc aagttttgat cggtagaaac aacaaacaaa atgataaact aactaaatat
     3541 atcgccaatg aaggtgacat ttggctccat actaaaaaga tagccggttc acacgtaata
     3601 ataagaaacc ataacaataa agaagtacca gaaagtacaa ttatcgaggc agcccgctta
     3661 gcagcctatt tcagtaaagc caaagaatct acaaacgtac ccattgatta tactcctgtt
     3721 gaaaatgtca acaaaccaaa aggggctaag cccggcttag tctattatga taactataat
     3781 actgtttatg tggatcccgc tgagaaagag gtgatagaaa aaattgaaaa ataaaagact
     3841 gatcttaata gttttgattt tttgtctgct attttccttt catagtctag cagaaactga
     3901 tttgaaaaaa ttaaattttg ataatgatgt ttatgtcatt gaaaactatg ataagtttaa
     3961 ggaaatgaag tcaccaaaac tggctctcgc cttaagtgga ggtggggcca gaggatttgt
     4021 caatataggt gtaataaaag ccttgcatga agcaggtatc tatcctgata taatcatagg
     4081 atcaagtatg ggcagtataa ttagcacttt atatgggagt ggtctaaatg ttgaagatct
     4141 cataaaaatt acttctactt taccttactc ttctcttttt gatatcaatt taataccggt
     4201 ttactctcta tttcagaccc gaaaatttaa ttatattata gaaaaaatct cgcctaaaaa
     4261 agatatagaa aactttcctt taaatactgc tttactgaat tttgacttag atagtggtca
     4321 caagtatatc acgagtaaag gtagaatttc agaaatttta caaagtactt attctatccc
     4381 attttactat cccataaata agagtaaagg gagatatcta gttgatcccg gcctggttga
     4441 aatggttccg gccaaagctg ccaaagccct ttcggctgat attgtaatag caacgacagc
     4501 ctttgatgaa cttccctatg aaacatataa atatcccatg cagtctttaa tgagaatgct
     4561 tcttttggtc caaaaaaaga attcgcaacc tattttaaat aactattcag atataataat
     4621 tcaaaatgaa gtgagtgatt ttagtttcaa agacttcgat cttattgaag aatttatcaa
     4681 gatcggatat attaatgcga tggataagat gcctgaaatt aaaaaattat taaaagaaaa
     4741 aaattatgtt agtactaaac ccgattttta tgattttgat gaaaaacagt atcaaaagct
     4801 taatacagat atcctctttg atagaataat atatcccgag acttatttta actatctgct
     4861 ttattacttt aataatcaat ccttttttga acaagaactc tttcgaaatc tcgtctatga
     4921 ctttcattat ggcttttatt acgaaaattc tcatttgaaa tttgaaacta tttttgatgt
     4981 taacgaagac cgtcatactg aactaaaatt aagatacaaa aaattaactg aaaacattga
     5041 ttttatcacg agactaaaaa ataatccttc ctataataac aatatagact atatcaatga
     5101 acttaaatac tttataaaga attcttcagt atcattaggt tgggcgcact ttagaaatga
     5161 taattattat tatcttaata ataatataaa attagaacgg ggaaatttcg actttgagaa
     5221 taatacagat atactattga ataaaaacaa aaaaatgcag ctgatcaatt caagtttatt
     5281 agattataat attagtgtaa attgggcttt tcaaaataaa tttgtatact ctaataccaa
     5341 tttattagga gatcccgata tttatcgggg ccaggacaat gaacagaaag agaaaacatc
     5401 actttctagt tcaattattt atagattacc attttccgaa tcacaagaat tcctctattt
     5461 tttctcatta gaagagttaa aaccttccct gtttattgat atagaatcta ccgatcaatt
     5521 aaatgcagca ctaggactta atttgaatat ggtatcttca gtaatggggc ttaaaccttt
     5581 aaatctagat ttaagtgctg cttatgataa aaatgctcaa tcagttatct ataacttctc
     5641 agtagatatc aaattttaaa aataaaaagc cctcactatt tattaaaata atagtcgagg
     5701 gcttttttgc tatatattta attaattttt ttaatcatct tcctctttag ctcttcgttc
     5761 aatgatcttt tcactaatat cttctggcac tttctcataa ttggaaaact ccatagtgaa
     5821 atgaccatat ccacctgtta ttgattttag atctgtagca tagctaaata gttcactttc
     5881 tggtactttc gctttaattc tttgtgtgcc atcctgtggt tccatacctg aaattttacc
     5941 tcggcgagag tttaagtcac ccataatatc tcccatgaag tcttccggta ctaccacagt
     6001 aacatccatg attggttcaa ggataaccgg ttttgccttt tccatagcct gtttaaatgc
     6061 ttttgaggca gcaattttaa aggccatctc tgaggaatca acatcgtgat atgaaccatc
     6121 atagacggta gctttaaagt caactaccgg ataatcagct aatattccac tatccttagc
     6181 ctctacaata cctttttcaa ctgcaggtat atactggctg ggaatagctc ccccaaagat
     6241 ctcttcatca aattcaaacc ctttgcctct tggtaaaggt tcaaatctaa tcataacatg
     6301 gccatattga ccgcggccgc cggactgctt tttatatttt acttcagtgg aaactttttt
     6361 ccggattgtt tctttatatt ctacttttgg ttccacttga ttaaatccaa catcaaactt
     6421 tcttttacag atgtcattaa caacagataa atgaacagta cccattcctt taattaataa
     6481 ttgtttggta actttattgt attcaacttt aaaggtggga tcttctaaag aatacctctg
     6541 aatggcagag gacatctttt cattgtcggc ctcatcagct ggatcggctg ctttaattaa
     6601 catcggttct ggaaattcta taggagcaat tttaacctct agatcttctt tggtgatagt
     6661 atctgaagtc tctaaatcat ctaatttagc tactgcacct atatctccag ctattaattc
     6721 ttcgacctga tcctgttctg aaccatttaa tttatagagt ttgctaactc tataattatc
     6781 ttttgttctg ggtaagtaaa tttcttcacc cttttcaatc ctacctgaat aaattctaaa
     6841 gattgatagt ttaccgatat aagggtcaac catagtttta ccgaccatac cgataaaggg
     6901 cccttcttca tttgtttcaa attcttgttc atcttctcct ccagttacct ttaatgtttc
     6961 cctatcagct gcggaaggag ctaacttaat aaggtagtcc ataaaggtat tcacaccgga
     7021 gttatgaagg gctgaacctg aaaacacggg gactatctct ttacttaaaa caccttttaa
     7081 aagggcattg ttcatctctt catctgaaat atcttcatct tctaaatatt tcatcattag
     7141 ttcttcatct aattcaatta ttgattcaac aagctctaat ttgtattctt ctaataattc
     7201 ctgctgcttt tcatttaaat ctgatgtaga ctcctctcca tccgttaatt caataaactt
     7261 ctcacttaag aggtcgataa ctcctttata ctcatcccct tctttgaaag gaatggtaat
     7321 tggaagtaaa gtatcttcga aattttcttc taattcacta aagacctctt taaagctcgt
     7381 accttctttg tcaaatttat ttataaaaac aaatcttgaa ttattgtttt caacagctat
     7441 atcccaaaca taatttgtat taacttctac accagaatcg gcattaatta ataaaactga
     7501 actttctgcc attctagtcg cactggctac ttctcctctg aaatctgcat agccgggtgt
     7561 atcaatcaaa ttaacctgct tttctttcag tttgaaagaa aaataggaac tgttaattga
     7621 atatttatga tccttttctt ccggtataaa gtctgatttt gtacttccct cgtcaactga
     7681 accaatctgt tcaatttttc ctgcattaaa taatacgtcc tctgttaaag tggtttttcc
     7741 cgcacctcca tgggaaataa atgaaaagtt tttaatatct tttgtcttat aattactcaa
     7801 aattttacac caaccttcct aattctaata ttaaagcttt taaagataga cacgcactct
     7861 tttttatttc tatcttaaca ttataaatcc tgcttaaaaa ttgtaattat ttttaattat
     7921 tctgtaaatt ttaaatatta ttaaactcat tatggtagac aaaatcttct aaatcttttc
     7981 tgggcggcct ttccttttca tctttagcct gtttttctga taaaatatct ttaatttcat
     8041 cagaatgttt gcctacaata actaaggtaa ttaaacggta atcttctggt attgacatta
     8101 cctcttttgc ctttgattca ctgtaacctg cgattgcatg ggccacaaga cctaactctc
     8161 gcgcccttaa catcagcatt gaagtagcta aaccagtatc aaaaagatag tattctctac
     8221 ccttgacatt acaatcatct tccttttttg caaagacacc tattaccatt gaagcattat
     8281 gagcccactg attaccgcta gataaagtat caaaaacctc atctaattta tcttcatcat
     8341 gtacaaatac atatcgccag ggttgattat taaaacaaga aggtgccaat ttggctgaat
     8401 tagctaagtc ttcgattaat tcatctgtaa tttcaacagg atcca
//
LOCUS       DATGCJ010000211        15843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_164159, whole genome shotgun sequence.
ACCESSION   DATGCJ010000211 DATGCJ010000000
VERSION     DATGCJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164159"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VKN64_09530"
     CDS             <1..541
                     /locus_tag="VKN64_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMA60521.1"
                     /translation="LSDKKFAGSDTWATAYTLSQAIKEAGDFDLIFCGRQAIDGDTAQ
                     VGPQVAENLDIPQITYVRELNIEGAKVTAKRKLEEGYSIIETELPVLLTVLGDLNRPR
                     YPSIKRIVDAFNNENTVKVWNADDFAVDDDKLGLDASPTQVYDTFVPTRDHEAEILEG
                     TVEEKVGKLIDKLKEEKVV"
     gene            566..1822
                     /locus_tag="VKN64_09535"
     CDS             566..1822
                     /locus_tag="VKN64_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMA60522.1"
                     /translation="MLKISADKCTGCKVCIDKCPFDALEIKNEIAVVDQASCTMCGIC
                     VKNCPEEAIKLEKEEKKKKDLSDYSGVWVLAEQRDNKLREVSFELVSEGRKLADELGE
                     ELSAVVLGSDINDTSKKLIAQGADNVYLIEDERLKDYRTGPYTSIISEMINKFKPEIV
                     LLGATHNGRDLGPRISARINTGLTADCTELSIEKERGILLQTRPAFGGNLMATIICPE
                     HRPQMATVRPGVMEKSEPDYDRVGDIKECKVDLDEDNIDSEVIKKSKIIKLDSNTSKL
                     DFYTKIKEIVKEAESNVNLEDADIIVAGGRGVGDPSGFSTIEKLAKTLNGEVGASRAV
                     VDEGWIDKEHQVGQTGKTVKPRVYIACGISGAIQHKAGMDSSDIIIAVNTDPEAPIFE
                     ACDYGIVGDLHQVVPMLAEAFDQAVN"
     gene            2163..3545
                     /locus_tag="VKN64_09540"
     CDS             2163..3545
                     /locus_tag="VKN64_09540"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022872 -
                     protein-N(PI)-phosphohistidine-mannitol phosphotransferase
                     system transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HMA60523.1"
                     /translation="MREKIQSFGRFLSGMVMPNIGAFIAWGLITALFIPDGWFPNESF
                     ANLVGPMINYLLPLLIGYTGGKLVHGRRGGVVGAIATMGVIVGASIPMFLGAMIMGPI
                     GGWVIKQFDNMVEGKIKSGFEMLVNNFSAGILGMLLSLLAYTVIGPSVTALNNVLRAG
                     VEFVVNAGLLPLASLFIEPGKVLFLNNAINHGILSPIGVQQVEEMGKSIMFLLETNPG
                     PGLGLLVAYWLAGKGNAKTTAPGAIIIHFFGGIHEIYFPYVLMKPSMIIAVIAGGMTG
                     VFTFVVTGAGLVATPSPGSIFAEMAMTPPGNFIPVLSGIALSAIVSFLVGWVILKRSP
                     NAETAGDLGAATSQMESLKGKKSSVSDTIKKDLKEVDRIIVACDAGMGSSAMAATQLK
                     NKLKEAGLGDIQVDNAAVNEIPLDTDIVLLHESLLERAKNRVPDAEFITIDNFMNSPA
                     YDQLVERLQS"
     gene            3626..5761
                     /locus_tag="VKN64_09545"
     CDS             3626..5761
                     /locus_tag="VKN64_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HMA60524.1"
                     /translation="MNLKNLSSREKKILKILIQAEEYLTAQELAEKIGVSKRTISREL
                     SKLKKAVKKSDLELKSKPSVGIFLTGEQDLVDEYSQQLRNFDSTFDYSPEGRKKYIIE
                     QFLFSSRFQKSTALAHRLAVTKATISYDLNKVADWFEKRGLNLTRKQGVGIELEGPEA
                     NFRKAIIDFLYEDIGEDNFLSLVRNRVNNKLDDVKPRENILQTKLDILNFISFETSSK
                     IEKGVKDILDDHQFQMVNSSYMGLIIHLALAIKRLESGDEIKVSQDKLEKLKKSKEYK
                     LSERIADKLEEIFNLEIPPDEKGYITMHLKGAKIRKDIEFIDEPLEDEELEAIKYARR
                     LVREVEKDLSFQLINDYSLISGLTTHLEPALSRLKMGLEIRNPVLDEIKEEYEEIFNI
                     TKGASQLLAEELNCEIPDSEIGYLTMHIASAIENINSSESQYRVLVVCSSGIGSSKIL
                     AARLHKALDNVKIVAETSALAVEEYMNNNIDIIISTVSLENYEDKTIIVSPMLTKNDI
                     KKINRKINKLNLNKIKRDLQPDVKDNKEKTFDEKLKVISKLSDDTLKLINEFRIINLG
                     NVNHHDEIIEKISKEKYFPANFELNKIKNELRKREEKGATIIPEMRLALMHSRVEELP
                     HPYLSLVTLANEIKIENTDREVTIDRVIVLLAPQNILKEQLDLLSRFSASIIENENFN
                     EIIHANSREGFLNYFREIMDNFFSDYVDM"
     gene            5815..6270
                     /locus_tag="VKN64_09550"
     CDS             5815..6270
                     /locus_tag="VKN64_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HMA60525.1"
                     /translation="MGFSDMFNKEKKEMLSKDLIKLDVKADSMEEAVEIAGRLLYENG
                     YVEEEYLEAMQEREKDVSTYMGNGVAIPHGTNESKKYIKSTGISFVQIPEGVDFGEGN
                     IAKLVIGIAGQGDEHLQILQNLATALQDEDKTSLLVRTEDKQVVLDSLL"
     gene            6297..7229
                     /gene="pfkB"
                     /locus_tag="VKN64_09555"
     CDS             6297..7229
                     /gene="pfkB"
                     /locus_tag="VKN64_09555"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HMA60526.1"
                     /translation="MIKTLTLNPAVDKTIIVEDFKIDNLNRVKEVYKDAGGKGINVSK
                     MINNLNGKTTATGFLGGYTGKYIKEELDKMNIEHDFIEVEKETRTNIKMVDRSKKTFT
                     DINENGALVSKEKIQRLKSSIFSNLKEDDILTLSGSVPKGLNDTIYAEIIEMAKEKKI
                     ETILDADGNLFAEGVKAGPTLIKPNDHELSMYFNKDFKNLDEMIKAAQRFFDTGIEMV
                     MLSLGEKGAVFLNKEKKIKINPLQITVKSTVGAGDAMVAGLALGMEKKLSAEEMVSLA
                     AAASSASLVNNGTEMGKKEEVEKFIEKIEISYIS"
     gene            7283..8530
                     /locus_tag="VKN64_09560"
     CDS             7283..8530
                     /locus_tag="VKN64_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMA60527.1"
                     /translation="MQTRAVRLYGENDLRLEKFELPSIKEDEILAKIVSNSICMSTYK
                     AVIQGARHKRVPADVAENPTITGHELAGEIIKVGEKWQDQFEPGQKFAVQPALNYKGS
                     PFAPGYSYKYFGGNTEYAIIPPEVMELGYLLKYEGDAYYEASLAEPMSCIIGGYHSVY
                     HTEDNNYIHKMGIVEDGKVALLGAAGPMGLGAIDYALHAEKRPAMILVADINQDRLDR
                     ASKLFSSEAKKQGIELKFVNTSGFDNVPNKLKEIGSGGFDDVFVYAPVKKLAEQGDQI
                     LGKDGCLNFFAGPTDKEFSAEINLYDVHYSAHHIMGSTGGNDDDMRESLKMSAAGNLN
                     PATMITHVGGLNSAAETIMDLPEIPGGKKLIYTGIDMELTAIKDFKEKGKDNQLFADL
                     AEIVEKNNGLWSAKAEKYLLENC"
     gene            8594..8887
                     /locus_tag="VKN64_09565"
     CDS             8594..8887
                     /locus_tag="VKN64_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HMA60528.1"
                     /translation="MAVKKTVKIKNSTGLHARPAALFVKKAGEFESDINVIFNDKEVN
                     AKSVMGVMSLGASKGKEIVIQAEGEDSQKAVEQLVKFVEYEMPKENESSEDEE"
     gene            8893..10602
                     /gene="ptsP"
                     /locus_tag="VKN64_09570"
     CDS             8893..10602
                     /gene="ptsP"
                     /locus_tag="VKN64_09570"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HMA60529.1"
                     /translation="MQGIAASPGIAIGKSLIKKDKNVEIDRNTISDEQIENEINKLHS
                     ALAKAKQSLKELKESTAAKLGEEKAEIFEAHMMILEDPEVVPAFEKKIKNEKINAAAA
                     VKDVIDKYVAMFSAMEDDYLRERGADIRDVGMRVIKILLDVEDITDKMEEDVVVIAKD
                     LTPSDTAQLDTDKVLAFVTEEGSRTSHTAIMARSLGIPAVVGVGDDLLKEANDNSQVI
                     VDGNNGRVYFSPSVATLDEYQEKLKEYNKEQKRLEYYKDKKAETKDGKEIELVGNMGN
                     LNDIKPIVNKGGEGVGLFRTEFLYMDRNELPTEEEQFEVYKEVAKRFEEKPVVIRTLD
                     IGGDKDLPYLNFPEEMNPFLGYRAIRFSLEREEIFKPQLRAILRASKFGNLKIMFPMI
                     SSLDELRKAKDKVNTIKTELEAEGIEYNKEIEIGIMIEIPAAVMIADHLAQEVDFFSI
                     GTNDLIQYTIAVDRTNEKIAERHTPYHPAVLRLIKKTIDYGHAQDIWVGMCGEAAGDE
                     LLLPFLLGVELDEFSMSAVSILKIKELMSKWNKVEAQEVAQKVLNLKTAGEVKSYLKD
                     IKK"
     gene            complement(10730..12250)
                     /locus_tag="VKN64_09575"
     CDS             complement(10730..12250)
                     /locus_tag="VKN64_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMA60530.1"
                     /translation="MKFGTNSFFSRLLSYNIIIVFITLLSISLVFGYLIQNYYYGLRE
                     WKTTNNGHRISEVVNEYIEGENLQRINKEESKKRLNTISNTTGMEIGIINNEGQYILN
                     SSNFESFNLEIENKSIKEVMSGNQVTRKIKGPEKNHLLMIFPLFKDPLKTADDVTIDT
                     GDKKRLEVIGGIVLETKLDDITTTLFDILKLILYASIVGLIIAVSFSIKFSKNIVKPI
                     NNLQNAAQNISNGKYTKVKENSINTEEIKNLVNTYNYAVEEIKNNIEKKKRLEKMRKE
                     YIADISHEFRSPLASINGFIEIITEEELDEEKLQKYSKIIKREADYLNYLVEELLLLG
                     QLDSEGYTITKEKTKLNKLIEFAVDSKRKEIKEKSLNIKIDLENEEININVDPNKFRE
                     VFLNLIENAVKYSPQGKTIKISSTHHYDENNNLKEIKIIISDQGKGIPEDKKEKIWQR
                     FYKLDAARTRDDKKSSGLGLSIVKEIIENHEAEIEVENNKESGPSFIITLKEENLL"
     gene            complement(12255..12974)
                     /locus_tag="VKN64_09580"
     CDS             complement(12255..12974)
                     /locus_tag="VKN64_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA60531.1"
                     /translation="MNLNDKKFLLIEDDENIFELIKAMLETYNTEITFRTDGKSGYQE
                     ALTGDYDLLLLDIMLPKMDGWEICKQLKENDINTPIIMLTAKVEETDKVLGLEMGADD
                     YVTKPFSPRELRARIKAVLRRFENANQINKKENTNKFTFPKINLVINYQGKSVVYNGK
                     SINLAPKEFDLFTFMAQNKDTAFTRKELMEEVWGKDHSEEMRTIDEHIKRIRRKLKNQ
                     GMQETPLKTVWGTGYKFEIEG"
     gene            13171..13797
                     /locus_tag="VKN64_09585"
     CDS             13171..13797
                     /locus_tag="VKN64_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60532.1"
                     /translation="MAKEEKSTKEKIYKYVKVGVVIVFVFWLVFAINGVQNQAKENKN
                     VILKPEYEVTYLPGGPERFVGGYRDVVNEEKKKRINTDNYLWTTLLVKNEGNEDAKDI
                     NLTINSAYPMAKVLVNPSGYMNEVDLNTNKDNLTTKIEIADLEIDNKAFVFIGFKPAN
                     IEKPYDQNDMVKWAENYRNYLQKVNMESFNTEDTYYVTGYREIFNTGS"
     gene            complement(13859..14224)
                     /locus_tag="VKN64_09590"
     CDS             complement(13859..14224)
                     /locus_tag="VKN64_09590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMA60533.1"
                     /translation="MQCPFCETGERVLKNDRAFALYDKYPVNQGHLLIITKRHVASYF
                     DITHAEKKALDDLIIKGKELLEEKYNPVGYNIGLNEGKYAGQTIKHLHIHLIPRYEGD
                     IEDPTGGVRGVIPDKRIYP"
     gene            14399..14812
                     /locus_tag="VKN64_09595"
     CDS             14399..14812
                     /locus_tag="VKN64_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HMA60534.1"
                     /translation="MAEIKSLLQANNLKNTGQRRKILKTLYNEDKPLSAKEIFFKLKK
                     DNPKLKLSTIYRNLNKFTEKDIVKKLNIKNEKHENLFEVKTDRHHHHLICVECGDIKE
                     LGCPLDDYLKKVAKENNYKIKDHRLTIYGICNKCK"
     gene            14851..15165
                     /locus_tag="VKN64_09600"
     CDS             14851..15165
                     /locus_tag="VKN64_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60535.1"
                     /translation="MNRMEILNLKEGKLLKLVNEKFDTDIDSIIDEDNLSEAWTIAQK
                     LLQKGYRVDVQAYPEYKKIEGIQIESGSPTTIFARYGSTPNFKTVVEGICKLGLIIEE
                     EA"
     gene            15228..15575
                     /locus_tag="VKN64_09605"
     CDS             15228..15575
                     /locus_tag="VKN64_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMA60536.1"
                     /translation="MYKVNQDLCIGCQQCIEACPAGAISIVNNKAQINPDLCRECGNC
                     LQACPEGAIYNDTSYQKQEIDNGSFNQRRPFSQKGIKRRPRRGLNQKKGRGNKRGPGK
                     GRVLGRGKGKKNN"
     gene            15610..>15843
                     /locus_tag="VKN64_09610"
     CDS             15610..>15843
                     /locus_tag="VKN64_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60537.1"
                     /translation="MGNLIEANINKIFNEIPDSVMVVGAAKSQSPLDVKKAVEAGLEI
                     IGENYIKDAKEVYHRIKDKAQLHFIGIPDKAKHD"
BASE COUNT         5497 a         2307 c         3185 g         4854 t
ORIGIN      
        1 gctttccgat aaaaagtttg caggttcgga cacctgggct accgcctata ccttatctca
       61 ggcaattaaa gaagcaggag attttgatct cattttctgc ggacgtcagg ccattgatgg
      121 tgatacagcc caggtaggac ctcaggttgc tgaaaattta gatattcccc agatcacata
      181 tgtacgggaa cttaatatag agggggcaaa agtaactgct aaaagaaaat tagaagaggg
      241 ctatagtata atagagaccg aattaccggt cctacttaca gtattaggag atttaaatag
      301 accaagatac ccttcaataa agaggattgt tgatgccttt aataatgaaa acacggtaaa
      361 ggtttggaat gctgatgatt ttgcagtcga tgacgataaa ttaggtttag atgcttcccc
      421 gactcaggtc tatgatacct ttgtgcccac cagagaccat gaggcagaaa tcttagaagg
      481 tactgttgaa gaaaaagtag gaaaattaat agacaaatta aaagaagaaa aggttgttta
      541 gtaatcttga aggaggcttt tttcaatgtt aaaaatcagt gcagataaat gtacgggttg
      601 taaagtttgt atagataaat gtccttttga tgctttagaa ataaaaaatg agatagcagt
      661 tgttgaccag gccagctgta caatgtgtgg gatctgtgtc aaaaactgtc ctgaagaagc
      721 tattaaatta gaaaaagaag aaaaaaagaa gaaagatctc tcagattaca gtggggtctg
      781 ggtcctagca gaacagcgcg ataataaatt gagggaagtt tcttttgaat tagtgagtga
      841 aggtagaaaa ctggccgatg aattgggtga agagctttcg gcagttgtac tgggttctga
      901 tattaatgat accagcaaaa aattaattgc tcagggagca gataatgttt acttaataga
      961 agatgaaagg ttaaaagatt atcgaacagg tccctatacc agtattattt ctgaaatgat
     1021 caataaattt aaaccggaaa tagttttatt aggggctacc cataatggtc gggatttagg
     1081 accgagaatc tcagccagaa ttaataccgg tttaaccgca gactgtacag aactttcaat
     1141 tgaaaaagaa agaggtattt tactgcagac cagacctgct ttcggtggta atttaatggc
     1201 caccattatc tgtccagagc ataggcccca gatggccacc gtcaggccgg gtgtaatgga
     1261 aaaatcagag cctgattatg accgggtagg agatataaaa gaatgtaagg ttgatttaga
     1321 tgaagataat atagattcgg aagtaattaa aaaatctaag attattaaat tagattctaa
     1381 tacttctaaa cttgactttt atacaaaaat aaaagagatt gtaaaagagg ccgagagtaa
     1441 tgtaaattta gaagatgccg atataattgt ggcaggtggc cggggagtag gagatccctc
     1501 cggttttagc actattgaaa aacttgctaa aaccttaaac ggtgaagttg gtgcttcgcg
     1561 ggctgtagtt gacgaaggct ggattgataa agaacatcag gttggacaga ccggaaaaac
     1621 cgtaaaacca agagtttata ttgcctgcgg tatttcaggt gccatccagc ataaagccgg
     1681 tatggatagt tctgatataa ttattgctgt caataccgac ccggaagctc caatttttga
     1741 agcgtgtgac tatggaattg taggtgattt acaccaggta gtgccgatgt tagcagaagc
     1801 ctttgaccag gccgttaatt aatatataag aaaataagat attatacttt gtaggctgga
     1861 atcttagttg attccagctt ttttctttaa tgtaaaaatt taattatata tttttttaca
     1921 tatttaataa agttctcttc cttaaatata ttgataatat agtagtcatc cctattaatg
     1981 ttaaaagttt gttaaaaaat gatgacagtt attaaaaaat gtcattaact gttagtgtca
     2041 aacggttata tttcaatcct tgtgcaaatt gtctgaatat tgtaccattg aatcagatta
     2101 aagagagttg tattattaat tttttattaa tttaataata atcaaaagag ggagtgtagt
     2161 aaatgagaga gaagattcaa agttttggtc gatttttgag tggtatggta atgcccaata
     2221 ttggggcttt tatcgcctgg ggccttatta cagctttatt tattccggat ggatggtttc
     2281 caaatgaaag ttttgctaat ctagttggcc cgatgataaa ttacttatta ccactgttaa
     2341 tcggttatac tggtggtaag ttagtgcacg gcagacgcgg cggagtagtc ggtgctatag
     2401 caaccatggg tgtaattgtt ggtgccagta ttccaatgtt cttaggtgcc atgatcatgg
     2461 ggccgattgg tggttgggta attaaacagt ttgataatat ggtagaaggc aagattaaat
     2521 ctggttttga aatgttagta aacaactttt cagccggtat tttaggtatg ttactcagtc
     2581 tattagctta tactgtaatt ggaccttctg taacagctct aaataatgtt ttaagagctg
     2641 gagttgaatt tgtggttaat gccggcttac tgccactggc ttcattattt atcgagcccg
     2701 gcaaagtact atttttaaat aatgcaatta accacggtat tttaagcccg atcggtgtcc
     2761 agcaggttga agagatgggt aaatctatta tgttccttct ggaaacaaac cccggtcccg
     2821 gtttaggttt acttgtagct tattggttag ccggtaaagg aaatgctaaa accaccgcac
     2881 ctggtgcaat cataattcat ttcttcggag gtatccatga aatctatttc ccatatgtac
     2941 tgatgaagcc ttccatgata attgcggtaa tcgcaggtgg aatgaccggt gtatttacct
     3001 ttgttgtaac cggagctgga cttgtagcaa ctccttcgcc tggtagtatc tttgccgaga
     3061 tggcgatgac tcctcccggc aactttatcc cggtactttc cggtatagca ttatcagcga
     3121 tcgtttcatt tttagttggt tgggtaatat tgaagaggtc tcccaatgca gagacagctg
     3181 gcgacttagg tgcagccacc agccagatgg aaagtctaaa aggtaaaaag tcttccgtat
     3241 ctgatacaat taaaaaagat ctcaaagaag tagatagaat tattgtggcc tgtgatgcag
     3301 gcatgggttc aagtgcaatg gctgcgactc agctaaagaa taaattaaaa gaagcaggtt
     3361 taggagatat tcaggtagat aatgcagctg taaatgaaat tccgcttgat acagatatcg
     3421 tcctcttaca tgaatcatta ctagagagag ctaaaaacag ggtgccagat gcagaattta
     3481 ttacaatcga caacttcatg aacagccctg cttatgatca attagtagaa agattacagt
     3541 cataattttt aaaattatgt tataatggaa gcggtaggtt tttaactacc gcttccattt
     3601 accacattag aaacggggta aaagaatgaa tttaaaaaat ctttcttcac gggaaaagaa
     3661 aatattaaag atcctgatcc aggcagaaga atatcttacc gcccaagaat tagcggaaaa
     3721 aataggagta agtaagagaa ctatatctag agagctttcc aaattaaaaa aggcagtaaa
     3781 aaaatcagat ttggaactaa aatccaaacc gagtgtaggg atatttttaa caggagagca
     3841 agatttagta gatgaatata gccaacaatt acgtaatttt gattctacct ttgattattc
     3901 gcctgaaggc aggaaaaaat atataattga acagtttctg ttttctagcc gttttcaaaa
     3961 atcgactgct ttagctcacc gattagctgt aaccaaagct acaatcagtt atgacttaaa
     4021 taaagtagct gattggtttg aaaaaagagg gctaaattta acgagaaagc agggagttgg
     4081 tattgagtta gagggtccag aagctaactt taggaaagca attattgact ttctttatga
     4141 agatattgga gaagataatt ttctatcatt ggttagaaat agggtgaata ataaattaga
     4201 cgatgtaaaa ccgcgggaaa atattttaca gaccaaatta gatattttaa attttattag
     4261 tttcgaaacc tccagtaaaa tcgaaaaggg agtaaaagat atattagatg atcatcagtt
     4321 tcagatggtt aacagttcct acatgggact tatcatccat ttggctttgg ccataaagag
     4381 attagagagt ggggatgaaa ttaaggttag ccaggataag ttagagaaat tgaaaaagtc
     4441 taaagaatat aaattgtcag agagaatagc agataaatta gaagaaatat ttaatctaga
     4501 gataccaccc gatgaaaagg gatatattac tatgcaccta aagggtgcca agataagaaa
     4561 agatattgaa tttattgatg agccacttga agatgaagaa ttagaagcaa tcaagtatgc
     4621 gaggcgattg gtcagagaag tagaaaaaga tttaagtttt cagctaataa atgattacag
     4681 tctaatttca ggtttaacta cccatctgga gccggctctt tccagactga aaatgggatt
     4741 ggaaataaga aatcctgttt tagatgagat aaaagaagaa tatgaagaga tctttaatat
     4801 tacaaaaggt gcttcccaat tattagctga agaattaaat tgtgaaatac cagattctga
     4861 gatcggttac ttaaccatgc atattgcttc tgctattgaa aatattaatt ccagtgaaag
     4921 tcaatatagg gttttagtgg tttgttccag tgggataggt agttcaaaaa ttttggctgc
     4981 cagactgcat aaagctttag ataatgtcaa aatagtggct gagacctcgg ccctagcagt
     5041 agaagaatat atgaataata atattgatat cataatttca acggtttctt tggaaaatta
     5101 tgaggataag accattatag tatcaccaat gttaactaaa aatgacataa aaaagataaa
     5161 cagaaagatt aacaagttga acttaaataa gatcaaaaga gatctacaac ctgatgtaaa
     5221 agataataaa gaaaagacgt ttgatgaaaa gctaaaagtg atttcaaagt tatcagatga
     5281 tactttaaaa ttgattaatg aatttagaat aattaattta ggtaatgtaa accaccatga
     5341 tgaaataata gaaaaaataa gtaaagaaaa atattttccg gctaattttg aactaaataa
     5401 aattaagaat gagttgcgga agagggaaga aaagggggca actattatcc ccgagatgag
     5461 attggcttta atgcacagtc gagttgaaga acttccacac ccctatttat ccctagtcac
     5521 cttggcaaat gagattaaga tagaaaatac ggaccgggaa gtaacaattg acagagtgat
     5581 tgtattgctg gcaccacaga acattttaaa agaacagcta gacttgttaa gtaggtttag
     5641 tgcttctatc attgagaatg aaaattttaa tgaaattata catgctaatt caagagaagg
     5701 gtttttaaat tattttcgag agataatgga taatttcttt tcagactatg ttgatatgta
     5761 aagataaagg ctttcaattt ataaattaat tataattaaa aggtggagat aaagatgggt
     5821 ttttcagata tgtttaataa agagaaaaaa gaaatgttat caaaagatct tattaaatta
     5881 gatgtaaagg cagatagtat ggaagaagca gtagaaatag caggacgttt actatatgaa
     5941 aacggttatg ttgaagaaga atatttggaa gctatgcaag aaagagaaaa agatgtatcg
     6001 acttatatgg gtaacggagt tgctattcca cacggaacca atgaatctaa aaagtatatt
     6061 aaaagtactg ggatctcctt tgtacagatt ccagaaggtg tagactttgg agaagggaat
     6121 atagcaaaac tggtaattgg gatagccggc cagggagatg aacatttaca gatcctgcaa
     6181 aatctggcta ctgccttaca agatgaagat aagaccagct tattggtgcg cactgaagat
     6241 aagcaggtcg tgttagacag cttattataa tatcttagca atgaggactg gttaaaatga
     6301 ttaaaacttt aacattaaat ccggctgtag ataaaacaat tatagtagaa gactttaaaa
     6361 tagataacct taatcgagta aaagaagtct ataaagacgc cggtggtaaa ggaataaatg
     6421 tttctaagat gataaacaac ttaaatggta aaactacagc aactggtttt ttaggaggtt
     6481 atacaggcaa atatataaaa gaagaactgg ataagatgaa tatagagcat gatttcatag
     6541 aagtagaaaa ggaaactaga acaaacatca aaatggtaga ccgcagcaaa aaaaccttta
     6601 ccgatattaa tgaaaatgga gctttagtat caaaagaaaa aatacaaagg ttaaaaagta
     6661 gtatattctc taatttaaaa gaggatgata ttttaactct ttccgggagt gttccgaaag
     6721 gattgaatga taccatctat gcagaaataa ttgagatggc aaaagagaaa aaaattgaaa
     6781 caattttaga cgcagatggt aatctttttg ctgagggtgt taaagcaggt cctaccctca
     6841 ttaagccgaa tgatcatgaa ttgagcatgt attttaataa agattttaaa aatttggatg
     6901 agatgataaa ggcagctcaa agattttttg acacaggaat agaaatggtt atgctttcct
     6961 taggagaaaa gggtgcagta tttttaaata aagagaagaa gattaaaatt aaccccttgc
     7021 aaattactgt taaaagtact gtaggagctg gagacgctat ggttgcaggc ttggccttag
     7081 ggatggaaaa aaagctgtca gctgaagaaa tggttagtct agcagcagca gccagcagtg
     7141 ctagtttagt caataatgga accgaaatgg gtaaaaaaga agaagtagaa aagtttatag
     7201 aaaaaattga aatttcatat atttcttaat gaattttgtt tagttcaatg ttcaaaacta
     7261 ttaaataagg gtgatatcaa taatgcaaac tagagcagtt cgattatatg gtgaaaacga
     7321 tctaagattg gaaaagtttg aacttccgtc tataaaagaa gacgaaatat tagctaagat
     7381 tgtttcaaat agtatttgta tgtccacata taaagctgtc atacagggag caagacataa
     7441 aagagtcccg gccgatgtag cagagaatcc tactataacc ggtcatgagc tagcaggtga
     7501 gatcatcaaa gtaggagaaa aatggcagga ccaatttgaa ccgggccaga agttcgcggt
     7561 tcagcctgct ttaaattaca agggaagtcc ctttgccccg ggttattcat ataagtactt
     7621 tggtggcaat accgaatatg caattatacc tcctgaggtc atggaactgg gatatttgct
     7681 caaatatgaa ggtgatgcct attatgaggc ctctctggcg gaaccgatgt cctgtataat
     7741 tggtggatac cattcggtat atcacacaga agataataac tatattcata agatgggaat
     7801 agttgaagat ggtaaggtag ctcttttagg agcagccggt ccgatggggt taggagctat
     7861 tgactatgcc ctacacgcag aaaaaaggcc cgctatgatt ttagtagctg atatcaatca
     7921 ggatagatta gatagagctt ccaaactgtt ttcatctgaa gctaaaaaac agggtattga
     7981 acttaaattt gtcaacacct ctggatttga taacgttcct aataaattaa aagaaatcgg
     8041 gagcggtgga tttgatgatg tattcgtcta tgcacctgtc aagaagctgg cagaacaggg
     8101 agatcaaatc cttggtaaag acggttgtct taactttttt gctggtccta ctgataaaga
     8161 attctcagct gagataaatc tttatgatgt ccattattct gctcaccata tcatgggttc
     8221 tacaggaggt aatgatgatg atatgcgcga atctctcaaa atgtcagctg ccggcaacct
     8281 gaatcccgct acaatgatta cccatgtggg cggacttaat agtgcagccg aaacaataat
     8341 ggatttacca gagatcccag gcggtaaaaa gttaatctat accgggatag atatggaatt
     8401 aactgcaatt aaagatttta aagaaaaagg taaagataat caactatttg ctgacttagc
     8461 tgaaattgtt gaaaaaaaca atggtctttg gtcagcaaag gcagaaaaat atttattaga
     8521 aaactgttaa taagaaagaa ttaacttaag atattttata tataaattat aaaaaattga
     8581 agggatgatt atattggcag taaaaaagac ggttaaaatc aaaaatagta caggtttgca
     8641 tgccaggcct gctgctttat ttgtcaaaaa agcaggcgaa tttgagtcag atattaatgt
     8701 aattttcaat gataaagaag taaatgcaaa aagtgtgatg ggagttatga gtttaggagc
     8761 aagcaaagga aaagagattg taattcaagc tgaaggtgaa gattcccaaa aagcagttga
     8821 acaacttgta aagtttgtgg aatatgagat gccaaaagaa aatgaatcca gtgaagatga
     8881 ggagtgattt gcatgcaagg tattgctgct tcacctggaa ttgcgatcgg taaatctttg
     8941 attaaaaagg ataagaatgt agagatcgac agaaacacaa tttcagatga gcaaattgaa
     9001 aatgagataa acaaattaca ttctgccctt gctaaggcca aacagtccct aaaagaatta
     9061 aaagaaagta ctgctgccaa gctgggtgaa gaaaaggcag aaatatttga agctcatatg
     9121 atgattttag aagatccgga agttgtacct gcttttgaaa agaagattaa aaatgaaaag
     9181 ataaatgcag ccgccgcggt caaagacgtt atagataaat atgtagctat gttttctgct
     9241 atggaagatg attacttaag agaacgcggg gccgatatac gtgatgtggg gatgcgggta
     9301 ataaaaatcc tcttggatgt tgaggatata acagataaaa tggaagaaga tgtagtagtg
     9361 atagcaaaag atttaactcc ttctgatact gctcagcttg atacagataa agttttagct
     9421 tttgtaaccg aagaaggttc ccggacttct catacagcaa ttatggccag atcgcttggt
     9481 attccggccg tagtaggagt aggggatgat ttattaaaag aagcaaatga taattcacaa
     9541 gttatcgttg atggtaataa tggaagagta tatttttctc ctagtgtcgc cactttagat
     9601 gaatatcaag aaaaattaaa agaatataat aaagaacaaa aaaggttaga atattacaaa
     9661 gacaaaaaag ctgagaccaa agatggtaaa gagatagaat tagtcggtaa tatgggtaat
     9721 cttaatgata tcaaaccaat cgtaaataag ggaggagaag gtgtcggcct ctttagaacc
     9781 gagtttttat acatggatcg aaatgagctc ccgactgaag aagaacagtt tgaggtctat
     9841 aaagaagttg ctaagcggtt tgaagaaaag ccggtcgtta ttagaaccct tgatattggg
     9901 ggagataaag atctgcctta tctaaatttc ccagaagaga tgaatccttt tttaggttat
     9961 agagctatca gattttctct ggaaagggaa gaaatattca aaccacagct cagggcaata
    10021 ttaagggcca gtaaatttgg taatctaaag atcatgtttc ccatgatttc atctttagat
    10081 gaactaagaa aagcgaaaga taaggttaat acgataaaaa cagaactgga agcagaaggc
    10141 attgaataca ataaagagat agagatcggt attatgattg aaatcccggc cgctgttatg
    10201 atcgctgacc acttagcaca agaagtggat ttctttagta ttggaacaaa tgatttgatt
    10261 caatatacaa ttgcagttga tagaaccaat gaaaagatag cagaacggca caccccctac
    10321 catccagcgg tattaaggct gataaaaaag acgattgatt atggtcatgc ccaggatata
    10381 tgggtaggta tgtgtggtga agcagctgga gatgaacttt tattaccctt tttattaggg
    10441 gttgaacttg acgagttcag tatgagtgca gtctctatct taaaaatcaa ggagttaatg
    10501 tcaaaatgga ataaagtaga agcccaagag gttgctcaaa aagtcttaaa tttaaaaaca
    10561 gctggagaag tgaaaagcta tcttaaagat attaaaaaat aatatacatt tctaaaagca
    10621 gaaagcccca tgcggggctt tttgttatct atatttacca aattaattat aatgactaga
    10681 aggatctata taaaatccac atttacttaa tattttaaat taaaatgctt tacaacaaat
    10741 tttcttcttt aagagttata ataaatgaag gaccagattc tttattattc tctacttcaa
    10801 tttcagcctc gtgattttca attatttctt ttactattga tagaccaagg ccgctgcttt
    10861 ttttgtcatc tctggttctt gctgcatcaa gcttataaaa gcgctgccat attttttcct
    10921 ttttatcttc cgggatacct ttcccctggt cacttattat aatcttaatt tcttttaggt
    10981 tattattttc gtcataatga tgagttgagg atatttttat agttttccct tgaggtgaat
    11041 attttactgc attttctatg agatttaaaa agacttccct gaatttattg ggatccacat
    11101 tgatatttat ttcttcattt tctaaatcta ttttgatgtt gagacttttt tccttaattt
    11161 cttttcgttt agaatcaact gcaaattcaa ttaatttatt taattttgtt ttttctttcg
    11221 tgatagtata gccctctgaa tctaactggc cgaggagaag cagttcttct actagatagt
    11281 ttaagtagtc agcttctctt ttaattatct tagaatattt ttgtaatttt tcttcatcga
    11341 gttcttcttc agttataatt tctatgaagc cattaataga ggctagagga gatctaaatt
    11401 catgggatat gtcagcaata tattcttttc tcattttttc taatcttttt ttcttttcga
    11461 tattattttt gatttcttct acagcatagt tataggtatt cactaaattt tttatttcct
    11521 ctgtgtttat actattttct tttactttag tatacttacc gtttgaaata ttttgggcgg
    11581 cattttgtaa attattaatt ggtttcacga tgtttttact gaatttaata ctaaacgaga
    11641 ctgcgattat taaaccgacg atagaagcat agaggatcag ctttaggata tcgaagagag
    11701 tggtcgtgat atcatctaat ttggtctcca atactattcc tccgattact tccaaacgtt
    11761 ttttatcacc tgtatctatg gttacatcat cagctgtttt gagcgggtct ttaaaaagcg
    11821 ggaatatcat aagtaggtga tttttttcgg ggccctttat ttttcgagta acctggttac
    11881 cactcatcac ttctttgata gatttatttt cgatttccaa gttgaaactt tcaaagttag
    11941 atgaatttaa tatatactga ccttcattat ttatgatacc aatttccata ccggtggtat
    12001 tggagatggt attcaggcgt tttttacttt cctctttatt aattctctgc aaattttcgc
    12061 cctcgatata ttcatttact acttctgaga ttcgatggcc attattggta gttttccatt
    12121 ctcttaagcc gtagtagtag ttttgaatca aatagccaaa tactagactt atactaagta
    12181 aagtaatgaa tacaattatt atattgtaac ttagaaggcg cgagaagaaa ctatttgtac
    12241 caaatttcat ttttttatcc ctctatttca aatttatagc cggtccccca gactgttttc
    12301 aggggtgttt cttgcatccc ctgatttttt aattttcgcc tgatcctttt gatatgttca
    12361 tcaatggttc tcatctcttc actatggtct tttccccaga cttcttccat cagttctttc
    12421 ctggtaaagg cagtatcttt gttctgagcc ataaaggtaa ataggtcaaa ttcttttggg
    12481 gcgagattta tacttttacc gttgtatact accgatttac cctggtaatt tatgactaga
    12541 ttgatctttg ggaaagtaaa tttattggta ttttcttttt tattaatttg attggcgttt
    12601 tcgaatcttc ttaaaaccgc tttaatcctt gccctgagtt cgcgggggga gaagggttta
    12661 gtaacataat cgtctgcccc catctctaac ccgaggactt tatcggtttc ttctactttg
    12721 gcagttagca taataattgg agtattaata tcattttcct ttaactgttt acagatctcc
    12781 caaccgtcca ttttaggcag cattatatcg agcagaagca ggtcatagtc tccagttagg
    12841 gcttcctgat aaccggactt tccatcggtg cggaaagtga tttccgtatt atatgtttct
    12901 aacatggctt tgatcagttc aaatatattt tcatcatctt caatcagaag gaatttttta
    12961 tcattaagat tcataatatg ctcctttcgt ttacacattg tttatatttt ctttattctt
    13021 tataaaccgt gaaagtcctt gttttgattt aaaaggccca atagaggtga ataattttac
    13081 atatagtttg atttttgttt atattttgtt tataattata aacaacaagc cagtgaagtt
    13141 ttaaaaacta tttaaatttg gagggataaa atggctaaag aggagaagtc tacaaaagag
    13201 aagatttata agtacgttaa ggttggagtt gtcattgtct ttgtattttg gctggtcttt
    13261 gctatcaatg gtgttcaaaa ccaggcgaaa gaaaataaaa atgtaatact taaaccagaa
    13321 tatgaagtaa cctatttacc gggaggtccg gaaagatttg tcggtggtta tagagatgta
    13381 gtgaatgaag aaaagaaaaa gaggattaat accgacaatt acctctggac gaccttgctc
    13441 gttaaaaatg aaggtaatga agatgcaaaa gacattaatt taacgatcaa ttctgcttat
    13501 ccaatggcta aggttttagt aaatccctct ggatacatga atgaagtaga tcttaataca
    13561 aataaagata accttactac taagatagag atagcagact tagagattga taataaggct
    13621 tttgttttta ttggttttaa acccgctaat atagaaaaac catatgatca aaatgatatg
    13681 gtgaagtggg cagaaaatta tcgcaattac ctgcaaaaag ttaatatgga gtcttttaat
    13741 actgaagaca catattatgt aacaggctat agagagatat ttaacaccgg tagttaagtt
    13801 gcagcattaa actaatatcc tcatgaggaa ccagcttgac taagctggtt cttttttttt
    13861 acggatatat tcttttatca ggtatgactc cccttactcc cccggtggga tcttcaatat
    13921 ctccctcata tctgggtatt aaatggatat gcagatgttt gatagtttgc cctgcatatt
    13981 tcccttcatt gagcccgatg ttatatccga ccggattata tttttcttcc aataactctt
    14041 ttcccttaat tatcaaatca tcaagcgcct ttttttctgc atgagttata tcaaaataac
    14101 tggcaacatg tctttttgtt ataataagta aatgaccttg attaaccggg tacttatcat
    14161 agagggcaaa agccctatca ttttttaaaa ctctttctcc tgtttcacaa aaaggacatt
    14221 gcaagataat caccccattt ttattttata ttatatagat taaagattga tagattactt
    14281 tccttgttta aatatttgac atcccagctt atattaaata tacttaacat gtaatgataa
    14341 tgcaaatcat ttgcaataat atattaatat agataggagt gtgaagcagt tgaaagaaat
    14401 ggctgagata aaaagtttgc tgcaggctaa taacttaaag aatacaggtc agcgcagaaa
    14461 aattttaaag acattatata atgaggataa accactttca gctaaagaga tcttttttaa
    14521 gttaaaaaaa gataatccca aactaaaatt atctactatc tatcgcaatt taaataaatt
    14581 tactgaaaaa gatatagtta agaaattaaa tataaaaaat gagaagcacg aaaacctatt
    14641 tgaagttaaa acagataggc atcaccatca tctaatatgt gttgaatgtg gagatattaa
    14701 agaattagga tgtccgcttg atgattatct taaaaaagtg gctaaggaaa ataattataa
    14761 gattaaagat cacagactaa ccatctatgg aatctgcaat aaatgtaaat aaaattaaat
    14821 tcaataatag ataagtggag gtataaaaaa atgaacagga tggagatatt aaacttaaag
    14881 gaaggcaagc ttttaaaatt agttaatgaa aaatttgata ccgatattga tagtattatt
    14941 gatgaagata atttatctga agcctggact atcgcccaaa aacttttaca aaaaggttac
    15001 agggttgatg tacaggctta tccagaatac aagaaaatcg aaggtataca gatagaaagc
    15061 ggtagtccca ccactatctt tgcaagatat ggaagtaccc ctaattttaa gactgttgtg
    15121 gaaggaattt gtaagcttgg tctgatcatt gaagaagagg cataatgaaa tattgttcta
    15181 agaataaaat aattttttaa aataccacaa ataaaaaggt gatactcatg tataaagtta
    15241 atcaagatct ttgtataggc tgtcaacagt gtatagaagc ctgtccggcg ggggcaattt
    15301 ccatagttaa taataaagcc cagattaatc ctgatttatg tagggaatgc ggcaactgtc
    15361 tacaggcttg cccggaaggg gcaatatata atgatacaag ttatcaaaaa caagaaatag
    15421 ataatggttc ctttaatcag agaagaccat tttctcaaaa agggattaag agaagacccc
    15481 ggagaggttt aaatcaaaaa aagggaagag gtaataaaag aggacctggt aaaggtagag
    15541 tcttaggtcg gggtaaaggt aaaaagaaca attaattaat acctgcttaa caaaagggag
    15601 gttccatata tgggaaattt aattgaagct aacataaata agatatttaa tgagatacct
    15661 gactcggtaa tggtagtggg agcagctaaa agccaatccc ctcttgatgt taaaaaagca
    15721 gtagaagccg gcctagaaat tattggcgaa aactatatta aagatgcaaa agaagtctat
    15781 cacagaatta aagataaagc acagctgcac ttcatcggaa taccggataa agcaaagcat
    15841 gat
//
LOCUS       DATGCJ010000212        14194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_656594, whole genome shotgun sequence.
ACCESSION   DATGCJ010000212 DATGCJ010000000
VERSION     DATGCJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656594"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VKN64_09615"
     CDS             complement(<1..650)
                     /locus_tag="VKN64_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMA60538.1"
                     /translation="MSDKSASFLKGAFILSLAGILSKVMGFGYRIILSRILGDEGIGL
                     YQMAYPIYATLLVVSRSGIPIALAKLISDRVAKDQRKAAFKIFVVGRKLSIVVGLFFS
                     ILMAVMAKPLVNMLNWDPRAFYAVLALSPAIFFVSIMATYRGFFQGLQNMVPTAYSQV
                     LEQLVRMITMIGLVYYLIRYGLGYAAAGATFGAVTGAIAGLILVMKIYSNKKTEIFS"
     gene            complement(657..4157)
                     /gene="mfd"
                     /locus_tag="VKN64_09620"
     CDS             complement(657..4157)
                     /gene="mfd"
                     /locus_tag="VKN64_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMA60539.1"
                     /translation="MLNTENIFQKDKIQNLNKLTKSNQKVKVVGPEEACFSFVVSNII
                     QKLNKKVLIITHDNSRIETIYEDLVRVLDKERVSIFPEIETLPHEKINPDFNILKERI
                     RVLNEMQFASNENIVITSGTSILRKLMPKEYFEKKSINININSEINLNEVSSRLVQLG
                     YEREKMVEEPGQFSIRGGILDIYTLIYDDPVRIELFGDEVDSIRFFNVETQRSVENID
                     DITITPVNEIVFDKKQIEKFLPKIKRDYKKQISRLENTDSGEEAKFLKELSGENLDKL
                     SELHYFEGYEQFLPYFYEGKLSTIFDYMDNDFYVIYDNSERVWQRIKSYHRDLEETII
                     SLLEQGNILPSYGNIFLSEDDYLSFDKGMEGLYFTDEIKESPLNQIDDKIRFKTAGVE
                     SFNGQFELLKNRLTELSKKDYTVLLTLNTESKAKKLNDYLRENDIKSIYSNNIHLGRI
                     NVTDESFSSGFMLDDLKLAVFSEREIIGKKQQRKRKISDFEEGVRISSVNELSKGDYV
                     VHENHGIGKYLGVKTLEVQNKHQDYLMIKYKDTDKLYVPTEKIDLVQKYIGADSQPPK
                     LYKLGGNEWQKVKQRVQNSVKKMAIGLLELYAEREMTDGYDFSEDTKWQKDFENDFPH
                     ELTPDQFKAIEDVKEDMESDTPMDRLLCGDVGYGKTEVAIRAAFKAAMDEKQTAVLVP
                     TTILAQQHYNTFQDRIDQYPVRVEMLSRFRTQAEQKEILKQLAKGNIDILIGTHRLIS
                     KDVNFNDLGLLVVDEEQRFGVAHKEKLKSLKSNVDVLTLTATPIPRTLHMSLIGVRDM
                     SVIETPPKDRYPIRTYIREFDEQLIKDAVNKELSRNGQVYFVHNRVEDIDRKADMVGN
                     LVPDARIAVAHGQMSERKLENIMMDFYEGRYDVLVCTTIIENGLDISDVNTIIINRAE
                     YMGLAQLYQLRGRVGRSNRIAYAYLLYEEDRILSEVANKRLKAIKEFTSLGSGFKIAM
                     RDLEIRGAGNLLGAEQSGHIAAVGFSLYTKLLDSAIQDLKGQKEEAKDVEIEIDIDAY
                     IPDSYISDSRQKIEMYKKLKKADSEADIISLIDEFVDRFGDPPKPVMNLIELSRLNAT
                     AKKLGVKNISESSAGYRVIFREDNKIEGDYIVNLLNEYPKKLKVRNAASPEIILKDKK
                     ISILKKILKFLKRQVFSIEE"
     gene            complement(4220..4780)
                     /gene="pth"
                     /locus_tag="VKN64_09625"
     CDS             complement(4220..4780)
                     /gene="pth"
                     /locus_tag="VKN64_09625"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMA60540.1"
                     /translation="MNLKLIVGLGNPGKKYAETRHNVGFMAIKQLADEFGIVNVDKEC
                     EALVADGEIEDTKVILAQPLTYMNKSGRAVSKLIDKYDISLKDLIVIYDDLDLPAGKI
                     RVKGSGGSGGHKGIKSIIEYLDSIQITRIRIGIGRPLEIDVTEYVLHEFDKDQQKRID
                     QALKKVDNIIIELIKNDLDSTMNKFN"
     gene            complement(4856..5539)
                     /locus_tag="VKN64_09630"
     CDS             complement(4856..5539)
                     /locus_tag="VKN64_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HMA60541.1"
                     /translation="MHRYSIEVEKREKTGKGVARELRRQGLIPGVVYGRSREPQALTV
                     DPNDLKGKLYSNAIIDLDIDGEEETVMIKDFQKDVIKEEIIHVDFQHISMDETISITV
                     PIKLVGDAPGVREGGVLQQLMREVDIEVLPTNIPDEIELDIDELTLSDSLEVGDLDVP
                     EEVNVLNSPDDVIVTVVAPSEEIEEEEEEELEEEFIEPEVIGEEEEEEGEEGEEEEEN
                     IEDKDYYTQ"
     gene            complement(5564..6514)
                     /locus_tag="VKN64_09635"
     CDS             complement(5564..6514)
                     /locus_tag="VKN64_09635"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMA60542.1"
                     /translation="MSTYSDKLKIFAGNSHPALAEDICEYLGTELLDARAKRFADGEV
                     SVEINETVRGADVFIIQPTSPPVNENIMELLVIVDALRRASARRITAVVPYYGYARQD
                     RKAKPRDPITAKLIANILTAAGARRLLSIDLHAPQIQGFFDIPVDNLYAAPLMIDYFK
                     KKDLDNLTAVAPDVGSVKRVRSIARTLNIPMSIIDKRRPKPNVAEVMHVVGQSNIKDS
                     NVILFDDIIDTGGTITQAANALKENGAKQVFACASHGLFSGPAIERLENSCIDEVIVT
                     DSIPQKHSEGLDNLTVLSLAPLVGEAIDRIFKDLSVSVLF"
     gene            complement(6526..7884)
                     /gene="glmU"
                     /locus_tag="VKN64_09640"
     CDS             complement(6526..7884)
                     /gene="glmU"
                     /locus_tag="VKN64_09640"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HMA60543.1"
                     /translation="MSDLLTIVLAAGKGTRMKSDKNKVLHKIAGKEMVNHVIDTASEI
                     SSSVVCIVGHQAEAVKEAVNAENVDFKLQAEQLGTGHAVMQAEENIKKHDGDVLILYA
                     DIPLIKKNTINQMTDCHNDNSADLTIMTTLLKNPAGYGRIIKNEDSEIVKIVEEDDAD
                     QKEKMINEINSGIMCIDSKSLDRALDSLDNNNAQNEYYLTDIVDYIKDNNGKILPFQI
                     EDSEEITGVNDRVNLSKAEKLLRGRINKRHMENGVTIIDPETTYIDEKVEIGSDTVIY
                     PFTYIEGNTKIGRHNTIKPHNHIIDCKIGNNNDIKNSNVIKNSEIENGCNIGPFAHIR
                     PGCIIADGVKVGDYVELKKAEIATKTKVPHLAYVGDAEIGSETNVGAGTIFANYDGVN
                     KHKTIIGNSVFIGSNSTLVAPLRIKDRGKTGAGAVVTKDVEEDTTVIGVPAREYMKTK
                     DD"
     gene            complement(7897..8727)
                     /locus_tag="VKN64_09645"
     CDS             complement(7897..8727)
                     /locus_tag="VKN64_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60544.1"
                     /translation="MTPYVSDKDLYKFKLALILGNGNRLRKLLRRFPTEKKFKNAKSS
                     EIAETIGINDSESEVLSQLINIDEVFNEMVTFKPDQRWSKKPDATKIMGIDTEYLKSE
                     LDCIQYTVMDNFQIIDNGFIFTNGKLAPSVSIKEGIDFLKTVIDKHNPELIVGHNFNS
                     DISILENAYMRDIPELYYYDDTMNLMQWSNLSNIMGSAGLNNVVTNLFNLEVVDLFTA
                     YENLSLLMEYGIKDTIYPILIRYFVINNEMPDLDINFEINNVIKDENRDLLKSTSINL
                     "
     gene            complement(8801..9319)
                     /locus_tag="VKN64_09650"
     CDS             complement(8801..9319)
                     /locus_tag="VKN64_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HMA60545.1"
                     /translation="MRGDIKYQIRNLSIKNLSTRLISFLAMFTAFVAVGTYLHIPGPS
                     SSYFNLGEVAIYIIALIFGSRAGGIAGALGSSLMDIFLGYSLWAPFTFVIKGLEGFVV
                     GKLAKEGDLKSNIFAIIIGGNIMVVGYAITKGFLISWPAVIPEIGIDYAQMIIGGLVA
                     LPLSRQINNLLS"
     gene            complement(9340..10671)
                     /gene="glnA"
                     /locus_tag="VKN64_09655"
     CDS             complement(9340..10671)
                     /gene="glnA"
                     /locus_tag="VKN64_09655"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HMA60546.1"
                     /translation="MSKMSKRDVLKKADELNVKFVRLQFVDILGIIKNVAITIDQLEA
                     ALDGEIMFDGSSIEGFTRIHESDMYLVPDYDTFAIFPWHKEEGSIARLMCDVYTPDGE
                     PFIGCPRTTLKKVIKEAKEMGYEMHAGPEPEFFLFEKDKEGNPTTITNDNGGYFDLSP
                     VDLGEKARRDIVLALQEMDFEVEASHHEVAPGQHEIDFKYTPVLQTADNVATFKFVTK
                     RIAMEHNLHATFMPKPIFGENGSGMHVHQSLYKDGENAFYDPEDELGLSEIAYYYIGG
                     LLKHSKAYTAITNPIINSYKRLLPGYEAPVYITWSTQNRSALVRVPSARGEGTRLELR
                     NPDPAANPYLAMAVTLKAGLDGIKNKIDPGEQTTDNIFDMDTVERQERGIESLPGDIM
                     KAVDYLNNDEVIKNTLGQHIYEHFVVAKEVEWHDYSTKVHDWELDQYLMKY"
     gene            complement(10762..11517)
                     /locus_tag="VKN64_09660"
     CDS             complement(10762..11517)
                     /locus_tag="VKN64_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMA60547.1"
                     /translation="MKFDALVIAGGKNDGALKDYSDKEYESLIEIAGKPMVQHVLNAV
                     EGVDEIENVVMVGPEELNKVSNKDYNILKPEGTLVDNIKRGIEALNEEHYIIILTSDI
                     PLVTSSIIKDFIKSCQQEKEHDFFYPLISKENNLKMYPEVERTYVHLKEGTFTGGNVV
                     MVSPSIIQGPLDWYDKLLSLRKKPFKMSQMLGFKIIIKFIFRQLTIPELEKKVSEMIG
                     HKCKALIVDYPEIGFDVDKPSDLKMMKEKYLKS"
     gene            complement(11533..12414)
                     /gene="ispE"
                     /locus_tag="VKN64_09665"
     CDS             complement(11533..12414)
                     /gene="ispE"
                     /locus_tag="VKN64_09665"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HMA60548.1"
                     /translation="MEKVKIPAYGKINLFLDIKNVREDGFHNLEMVMQSIKLHDNITL
                     VPSKKGINIITDSKEIPTDESNLASQAAKAVMQKTKIQKGVNIHIDKNIPVAAGLAGG
                     SADAAAVIKGMNQLFELDMDYKAMHELAAGLGSDVPFCIKGGTAFATGRGEILNWLSP
                     LKKKWVILIKPPQKVSTKEVYQLYDKKNVDLEIPVQRLIKIIKDKDSLDIKDGWANVL
                     EPVTKTIIDEIEVIEEKLIDQGFNFTMMTGSGPTVFSLIDKDQVDEAKEVVKNWDRPQ
                     DYVVLTETINEINSGPS"
     gene            complement(12483..13784)
                     /locus_tag="VKN64_09670"
     CDS             complement(12483..13784)
                     /locus_tag="VKN64_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA60549.1"
                     /translation="MRRDKLKNKVCKKIEENKNEIIDIVQQIYNNPETGYKEKEATAL
                     VKNAFQTLKLKPEENIAVTGVKTSIGETDNEPHLAVLGELDALICRDHPDADSETGAV
                     HACGHNNQIGIMLGVAFGLVKTNILNELEGQIDFMAVPAEEFIEIEYRDRLIEEGQLS
                     YLGGKQELIKRGYFDDIDIAMLVHSLDVGDMKDVIVGPSGNGFIGKKIKFIGEESHAG
                     GAPEEGINALNAAVLAINNINAQRETFKDSDKIRVHPVITKGGDVVNVVPSDVKMESY
                     VRGSTVDSVFDANKKVNRAIQAGSDAIGGEVEIKDIPGYLPLINYETLMEIFTNNLEG
                     LVPKDRVIKQKTFTGSFDMGDLSHIMPVLHPFFSGVSGGLHTRHFKTEDYELAIIKPA
                     KALAKTIIDILIDKEKVLKQLNKPPYSKEEYLAILSKMESN"
     gene            complement(13964..>14194)
                     /locus_tag="VKN64_09675"
     CDS             complement(13964..>14194)
                     /locus_tag="VKN64_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60550.1"
                     /translation="APTDGTYYLAVFDENKEKGKYVFAIGRKEKWKANEIVKLPKTWW
                     DTRMFMEKEKSTYAITIGIGVVGLYLISTLFK"
BASE COUNT         4196 a         2978 c         2052 g         4968 t
ORIGIN      
        1 gaaaatatct ctgtcttttt attactatat atcttcatca ctaatattaa accggcaatt
       61 gctcctgtaa ccgccccaaa agtggccccg gctgccgcat accccagacc atatctgatt
      121 aagtagtaaa caagtccaat catggtgatc attcttacca gttgttctaa gacctgggaa
      181 taggcggtcg gaaccatatt ctgcagtccc tgaaagaaac cgcggtaggt agccataatc
      241 gagacaaaaa agatggctgg tgataaagct aacactgcat aaaaagctct tggatcccag
      301 ttaagcatat taaccagggg tttagccata acagccatca ggattgaaaa gaacaaacct
      361 accacaatac ttaattttct tcctaccaca aatatcttga aggcagcttt gcgctggtct
      421 ttagccaccc ggtctgaaat tagtttggcc aaagcgatcg gtataccgga acgagatacg
      481 acaagcaggg tagcatagat aggataggcc atctgatata aaccgatacc ttcatcacct
      541 aaaattctac ttaaaatgat gcggtagcca aaacccatta ccttagataa gataccggct
      601 agacttaata taaatgcccc ttttaaaaaa cttgcactct tatctgacat aaacaactac
      661 tcctctatac taaatacctg cctttttaag aactttaata tctttttcaa tatacttatc
      721 tttttatcct tcaatattat ctcaggactg gccgcatttc tgacctttaa ctttttagga
      781 tattcattta atagattaac tatataatcg ccttctatct tattatcttc tctaaaaata
      841 actcgatagc ctgcagagct ttccgaaata ttttttacac ctaatttctt agctgtagca
      901 tttaatctgc tgagttcaat taaattcata acgggtttgg gtggatcccc aaatctatca
      961 acaaattcat caattaagga aataatatcc gcttcactgt ccgctttttt aagtttttta
     1021 tacatttcaa ttttttgacg cgaatcagag atataactgt cggggatata tgcatcgata
     1081 tctatttcaa tttcaacatc ttttgcttct tctttttggc ctttcagatc ctgaatagca
     1141 ctatctaata actttgtata tagtgagaaa cctactgcag caatatggcc gctttgttcg
     1201 gcccccagca aattaccagc tcccctaatt tctaagtcgc gcatggctat tttaaaacca
     1261 gaacctaaac ttgtaaattc tttaattgcc tttaaccttt tatttgctac ttccgataag
     1321 attctatctt cttcatataa taaataagca taggcaattc tattggaacg gcccacccta
     1381 cctctaagtt ggtaaagctg ggcaagtccc atatactctg ccctatttat tattatagta
     1441 tttacatcgg aaatatccaa tccattttca ataatagtgg tacatactaa aacatcataa
     1501 cgcccttcat agaagtccat catgatattt tctaactttc tctcgctcat ctgtccgtga
     1561 gcaaccgcaa ttctggcatc tgggactaaa ttaccaacca tatcggcttt ccgatcaata
     1621 tcctccaccc tattatggac aaaatagacc tgcccatttc tagacagttc tttattgacc
     1681 gcatctttaa ttaactgttc atcaaattct ctgatataag tccttatcgg ataacggtct
     1741 ttcgggggag tttcgataac actcatatcc ctaaccccga ttaaagacat atgcagagtt
     1801 cttggaatag gagttgcagt taaagttaaa acatccacat tagattttaa agatttgagt
     1861 ttttctttat gggccacccc aaaccgctgt tcctcatcaa ctaccaataa tcctagatca
     1921 ttaaagttca catcttttga aattaagcgg tgggtaccaa ttaaaatatc aatattaccc
     1981 tttgccagct gttttaaaat ttccttttgt tcagcctgag ttctaaagcg gcttaacatt
     2041 tctactctga ccggatattg atctattcta tcttgaaatg tattataatg ctgctgggcc
     2101 aagatagtag ttggaactaa aacagcggtc tgtttttcat ccatagcagc tttaaaggct
     2161 gctctaatgg caacctctgt tttaccatag cccacatccc cacataataa gcggtccatc
     2221 ggagtatcag attccatatc ttctttcaca tcttcaatag ctttaaactg atcgggagtt
     2281 aattcatggg gaaagtcatt ttcaaaatct ttctgccatt tagtatcctc tgaaaaatca
     2341 taaccatctg tcatttcccg ttcagcatat agctctaaaa gaccaatcgc catcttttta
     2401 actgaattct ggacgcgctg ttttaccttc tgccattcgt tcccacctaa tttataaagc
     2461 ttaggtggct ggctgtcggc tccaatatac ttctggacta aatctatctt ttcagtcggt
     2521 acataaagct tatcggtatc cttatacttg atcatcagat aatcctgatg cttattttgc
     2581 acttccaggg tctttactcc caggtactta cctataccat ggttctcatg gacaacatag
     2641 tcccctttag ataattcatt gaccgaggag atgcgaactc cttcttcaaa atcggaaatt
     2701 tttctttttc tctgctgctt tttgccgatt atttcacgtt ctgaaaagac agctagtttt
     2761 agatcgtcaa gcatgaaacc tgaagaaaaa ctttcatcag ttacattaat tctacctaga
     2821 tgtatattat tactatagat acttttaata tcattttctc ttagataatc atttaacttc
     2881 tttgccttag actccgtatt taaagttaaa aggacggtat aatctttttt acttaattcg
     2941 gttaagcggt tttttaatag ttcaaactgt ccattgaaag attcaacccc agcagtttta
     3001 aatcttatct tatcatcaat ctggtttaag gggctctcct ttatttcatc tgtaaagtaa
     3061 agtccctcca tacccttatc aaaacttaaa taatcgtcct ccgataaaaa tatattacca
     3121 taactaggta agatattacc ttgttctaaa agcgaaataa tggtttcttc taagtcgcga
     3181 tggtaagatt taattctctg ccagacccgt tcactgttat cataaattac ataaaagtca
     3241 ttatccatat aatcaaagat agtgctgagc tttccttcat aaaagtaagg caaaaactgt
     3301 tcataacctt caaagtagtg cagctcactt aatttatcta agttctcacc actgagttct
     3361 tttaaaaatt tagcttcttc cccgctatca gtattttcaa gtctacttat ctgtttttta
     3421 tagtctctct taattttagg taaaaacttt tcgatctgtt ttttatcaaa gacaatttca
     3481 ttgaccgggg taatagtaat atcatctata ttttcaacag atctttgagt ttccacatta
     3541 aaaaatctaa ttgaatctac ttcatcccca aatagttcaa tccggaccgg gtcatcatag
     3601 attaaggtat agatatccag tatgccgccc ctgatagaga actggcccgg ttcttctacc
     3661 atcttctctc gttcatagcc taattgaacc aaacgagagc taacctcatt taaattaatt
     3721 tcactattta tgttaatatt aatacttttt ttctcaaagt attccttcgg catcaacttt
     3781 cttaagattg aagttccgct tgtaataaca atattttcat tactggcaaa ctgcatttca
     3841 tttaagaccc taattctttc ttttaaaata ttgaagtcgg gattgatctt ttcatgaggt
     3901 aaggtctcta tttcgggaaa gatactaact ctttctttat ctaaaactcg cacgagatct
     3961 tcatagattg tctcaatcct gctgttatcg tgggtgatta tcaatacctt tttatttaat
     4021 ttttgtataa tattggaaac tacaaaagaa aaacaggcct cttctggccc cactacctta
     4081 actttttggt tgcttttggt tagcttattt agattttgaa ttttatcttt ttgaaagatg
     4141 ttttctgtat taagcatata ttcacctaaa tttttattat aaataccaaa atgaccccta
     4201 ctgaaaaggt acgggccttt tagttaaatt tattcattgt actatctaaa tcatttttaa
     4261 ttagttcaat tatgatatta tcgacttttt ttaatgcctg atcaattcgt ttttgttgat
     4321 ccttatcaaa ttcatgtaag acatattccg ttacatcaat ttctaaggga cgtccgatac
     4381 caattctgat tcttgttatt tgaatcgaat ccaaatattc aatgatggac ttaatccctt
     4441 tatgacctcc actacctcca ctgcctttta ctctgatttt cccggccggt aggtcaaggt
     4501 catcataaat tacgattaaa tccttaaggg aaatatcata tttatcgatt aatttagaaa
     4561 cggcccgccc gcttttattc atataggtga gcggttgggc taaaattacc ttggtatctt
     4621 caatttcccc atctgctact aaagcttcac actctttatc aacatttact ataccgaatt
     4681 catcagccaa ctgtttaata gccataaaac caacattatg tctggtctca gcatattttt
     4741 tgccgggatt acctaaacca actattaatt tcaagttcat aacctccagc cgacatatta
     4801 agaaaattaa gacgcaatcc cacatgggat tacgtctcta atttcatttt tgtttttact
     4861 gagtataata atctttgtct tcgatatttt cttcttcctc ttcgccttct tcgccttctt
     4921 cttcctcttc ttcaccgata acttctggtt caatgaattc ttcttcgagt tcttcttcct
     4981 cttcttcttc aatttcttcg gaaggagcaa caactgtaac gattacatca tcaggtgaat
     5041 tcagaacatt aacttcttca ggcacgtcta agtccccaac ttctaatgaa tcgctaagtg
     5101 ttaattcatc aatatcaagt tcaatctcat ctgggatatt agttggtagt acttcaatat
     5161 caacttctct cataagttgt tgtagtactc caccttctct tacaccaggt gcatctccca
     5221 ctaatttgat aggaacagta atactgattg tttcgtccat agaaatatgt tggaaatcaa
     5281 catgaatgat ttcttccttt ataacatcct tttgaaagtc tttaatcatt actgtttctt
     5341 cttcaccatc aatgtctaaa tcaataatgg cattactata aagtttacct tttaagtcat
     5401 tgggatcaac tgttaatgcc tgaggttctc tacttctacc ataaacaaca ccaggaatta
     5461 aaccttgtct tctgagttct cgagcaactc ctttaccagt tttctctctc ttttcaactt
     5521 caatactgta tctgtgcatg acataattca ctccttaata atattaaaat aatacactta
     5581 cagataaatc tttaaaaatt ctatcgatgg cttctcctac cagaggagcc aaagaaagta
     5641 cagttaaatt atccaagccc tcactatgtt tttgggggat tgaatctgta actataacct
     5701 catcaataca tgagttttct aatctctcaa tagcaggtcc tgaaaataaa ccgtggctag
     5761 cacaggcaaa tacttgttta gctccatttt ctttaagtgc attagctgcc tgtgtaatag
     5821 tgcctcccgt atcaataata tcatcaaata agattacatt tgaatcctta atattggatt
     5881 gacctaccac atgcatcact tctgccacat tgggtttggg acgtctttta tcaatgattg
     5941 acatcggaat atttaaggtt ctcgcaatcg atctaaccct tttcacagat cccacgtcag
     6001 gcgcaaccgc tgttaggtta tctaagtctt ttttcttgaa ataatcaatc atcaagggag
     6061 ctgcgtaaag gttatctacc gggatatcaa aaaagccctg aatctggggg gcatgtaaat
     6121 ctatcgaaag taatcttctc gctcccgcag cagttaaaat attggcaatt agtttagcag
     6181 tgatcggatc acgcggtttt gcttttctgt cctgtcgggc ataaccgtaa taaggtacta
     6241 ctgccgttat gcgccgggca gaagcacgtc ttaaggcatc cacgataact aaaagttcca
     6301 taatattctc gttaacaggt ggactggtag gttgaatgat aaaaacatcg gcacctctaa
     6361 ctgtttcatt gatttctaca cttacctcac catctgcaaa tcttttggcc cgggcatcca
     6421 gcagttcggt accaaggtac tcacagatgt cttcagctag ggctggatgg gaattaccag
     6481 caaaaatttt taacttatca ctataagtcg acatattatc cccctctaat catctttggt
     6541 tttcatatat tcacgggcag gaactcctat aactgtagta tcctcctcta catctttagt
     6601 tactacggca cctgcacctg ttttccctct gtcttttatt ctaagaggtg caacaagagt
     6661 agagttactg ccgataaata ctgagttccc tataatggtt ttatgtttat ttactccatc
     6721 ataattagca aaaattgtac ctgcaccaac attggtctca ctaccgattt cggcatcgcc
     6781 gacatatgcc aggtgaggaa ccttggtttt agtggctatt tcagcctttt taagttcaac
     6841 ataatcgccc actttcaccc catctgcaat tatacagccg ggtctaatat gggcaaaagg
     6901 ccctatgtta caaccatttt caatttcact attttttatt acattactat tttttatatc
     6961 attattatta cctatcttac aatctatgat atggttatgt ggtttgatag tattatgtct
     7021 gccaattttt gtatttcctt ctatataggt aaagggataa ataactgtat ctgaaccaat
     7081 ttctaccttt tcatctatat aggtagtttc agggtcaatt atagttacac cattttccat
     7141 atgcctttta ttaattctgc ctcgcaataa tttctcggct ttagataaat taactctatc
     7201 attaacacct gttatttctt cagaatcttc aatttgaaat ggtaggattt taccattatt
     7261 atcttttata taatcaacta tatctgttag ataatattca ttttgagcat tattattatc
     7321 aagactatct aaggcccgat ctaaactttt actatcaata cacattatac cactattgat
     7381 ttcatttatc atcttttctt tttggtctgc atcgtcttct tccactattt taactatttc
     7441 agagtcttca ttttttatta ttctaccata tccggcagga ttttttaaga gggtggtcat
     7501 tattgttaag tctgctgaat tatcattatg gcaatctgtc atctgattga tggtgttttt
     7561 cttaattaat ggtatatctg cataaagtat caaaacatct ccatcatgct tctttatatt
     7621 ttcttctgcc tgcatgactg catgacctgt acctaactgt tcggcctgca gtttaaaatc
     7681 tacattttcc gcattgacgg cttcttttac agcttccgcc tgatggccca caatacaaac
     7741 aactgaagaa gaaatttctg aagcagtatc tatgacatgg tttaccatct ctttaccagc
     7801 aattttatgt aataccttgt ttttatcaga cttcatcctg gttcctttac cagcagccaa
     7861 aacaatagtc agtagatccg acatttccaa tcctccttac agattaatag aggtgctttt
     7921 taaaagatct ctattttcgt cctttataac attatttatt tcaaaattaa tatctaaatc
     7981 cggcatttca ttattgatca caaaatacct gattaaaata gggtaaatag tatctttaat
     8041 accatattcc attaaaagac ttagattttc ataagctgta aataaatcaa ctacttccaa
     8101 attaaacaag ttggtgacaa cattattcaa accggccgat cccatgatat tagagaggtt
     8161 actccactgc atcaaattca tggtatcatc gtagtaatat agttccggaa tatctctcat
     8221 ataggcattt tctaaaatgc taatatcaga gttgaaatta tggccgacta ttaattcggg
     8281 gttgtgttta tcgataacgg ttttcaaaaa atcaatacct tctttgatag aaacagatgg
     8341 agctaacttg ccattggtaa agataaaacc attatctata atttgaaaat tatccatgac
     8401 tgtgtattga atacagtcca attcactctt taagtattca gtatcgattc ccattatttt
     8461 agtagcatcc ggttttttac tccacctctg atccggttta aaagttacca tctcattaaa
     8521 aacttcatct atattaatta attgagacaa tacttctgac tcagaatcat taataccaat
     8581 tgtctccgca atttctgacg atttagcatt tttaaacttt ttttcggtcg gaaatctccg
     8641 cagtaattta cgcagtctgt tgccgtttcc caaaattaaa gcaagcttaa atttatataa
     8701 atctttatca ctaacatagg gagtcataaa aaaatacctc aaatctaatt taaagtacca
     8761 tgattaaccg ttagaagtat aacagaatca ctttatattg tcaagataat aagttgttta
     8821 tctgcctcga taaaggtaga gcaactaaac caccaataat catttgagca tagtcgatgc
     8881 caatttccgg aataacggcc ggccagctga ttaaaaatcc tttagttatg gcataaccga
     8941 ctaccataat attaccgccg attatgatcg caaagatatt actctttaga tctccctctt
     9001 tggccagctt accaactaca aaaccttcta atcctttaat aacaaaggta aaaggggccc
     9061 agagagaata tcccaaaaag atatccatca aagaagatcc taaggcaccg gcaattccac
     9121 ccgcccgact gccgaatatc agggctatta tatagatggc tacctcccct aaattaaaat
     9181 aggaagaact aggcccggga atatgcaggt aagtaccaac agcaacaaaa gccgtgaaca
     9241 tagccaaaaa tgatatgagg cgagtagata gatttttaat tgaaagattc ctaatttgat
     9301 attttatgtc acctctcata acagtaaacc tcctgagaat taatatttca ttaagtattg
     9361 gtcaagttcc caatcatgta cttttgtact gtagtcatgc cactctactt cttttgcaac
     9421 tacgaaatgc tcataaatat gctgacctaa tgtatttttt ataacctcat cattatttaa
     9481 ataatctaca gccttcataa tatcaccggg aaggctttca atacctcttt cctgtctttc
     9541 cacagtatcc atatcaaaga tattatcggt agtctgctca ccggggtcaa tcttattttt
     9601 aataccatct aaaccggctt ttaaggtaac tgccattgct aaatatggat tggcagctgg
     9661 gtcagggttt ctaagctcca gtctggtacc ttctcctctg gcagaaggaa ctcttactag
     9721 agcactcctg ttttgagttg accaggtgat atatacgggg gcttcatagc ccggtaatag
     9781 tcttttatag gaattaataa tcggattagt tatggctgta taagctttgg aatgtttaag
     9841 taagccaccg atgtagtaat aggctatctc acttaaacca agctcatcct ccggatcata
     9901 aaaagcgttt tcaccatctt tatataaaga ctgatgaacg tgcataccag aaccgttttc
     9961 accaaaaata ggtttgggca taaaggttgc atgtaaatta tgttccatgg ctattctttt
    10021 ggtaacgaac ttaaaagtag caacattatc agctgtttga agcacaggtg tatacttaaa
    10081 gtcgatctca tgctgtccgg gtgccacctc atggtgagag gcctctactt caaaatccat
    10141 ctcctgtaag gctagaacaa tatcccttct ggctttttca cctaaatcta ccggtgaaag
    10201 atcaaaatag ccgccgttat catttgtgat agttgtggga tttccttctt tatctttttc
    10261 aaagaggaaa aattcgggtt ccgggccggc gtgcatttca taacccattt ctttggcttc
    10321 ttttattact tttttaaggg tggttctggg acaaccaata aatggttcac cgtccggggt
    10381 ataaacatca cacattaagc gagcaattga tccctcttct ttatgccagg gaaaaattgc
    10441 aaaagtatca taatccggta ctaaatacat atcggactca tgaattctgg taaaaccttc
    10501 aatagatgaa ccatcaaaca tgatctctcc atccagggct gcttctaatt ggtcaattgt
    10561 tattgctaca tttttaataa ttcctaaaat atcaacaaac tgtagtctga caaattttac
    10621 atttagttca tctgctttct ttaagacatc tctttttgac attttgctca tttattactc
    10681 actactcctc taatattttt ttataataat tatatttatt ttattataac aatatataaa
    10741 caaatatcaa ctaaaaatca attaagattt taaatatttt tctttcatca tctttaagtc
    10801 actgggttta tccacgtcaa aaccaatctc agggtaatct acaatcaggg ctttacattt
    10861 atgaccgatc atttcggaaa ctttcttttc taattccggg atcgttaatt gtctaaagat
    10921 gaacttaata atgattttaa atccgagcat ctggctcatt ttaaaaggct ttttccgtaa
    10981 agaaagtaat ttatcatacc agtctaaggg tccctgaata atactcgggc tgaccatcac
    11041 cacattgccg cccgtgaaag ttccttcttt taaatgcaca taagttctct ccacttccgg
    11101 gtacattttt aaattgttct cttttgaaat taaaggatag aaaaaatcat gctctttttc
    11161 ttgttggcag cttttaataa agtctttaat aatcgaagaa gtaactaagg gaatatctga
    11221 agttaaaata attatataat gttcttcatt taatgcttcg atccctcttt taatattatc
    11281 tactaatgta ccttctggtt taagtatgtt atagtctttg tttgaaactt tatttaattc
    11341 ttcaggaccg accattacaa cattttctat ctcgtcaaca ccttctactg catttaaaac
    11401 atgttgaacc atcggtttac cggcaatttc tattaaagat tcatattctt tatcggaata
    11461 gtcttttaag gccccgtcat tctttcctcc cgctatcacc agtgcatcaa atttcatttc
    11521 caacccccca atctaactag gtcctgaatt tatctcatta atggtttcag ttaaaactac
    11581 ataatcctgg ggtctgtccc agtttttaac aacttctttt gcttcatcaa cttggtcttt
    11641 atctattaac gaaaaaacag taggaccact acctgtcatc attgtaaaat taaaaccctg
    11701 gtcgattagt ttttcttcta ttacctcgat ttcatctatt atcgttttgg taacaggttc
    11761 taaaacattt gcccaaccgt cttttatatc aagcgaatct ttatctttaa ttatcttaat
    11821 aagtctttga acaggtattt ctaaatcaac attcttttta tcataaagtt gataaacctc
    11881 tttagtacta actttttgag gtggtttaat taagataacc cacttctttt ttaaaggaga
    11941 tagccaattt agtatttcac cccggcctgt agcaaaagcg gttcctcctt taatacaaaa
    12001 gggaacatca ctcccgagac cggccgctag ttcatgcatt gctttataat ccatatccag
    12061 ttcaaaaagt tggttcatac ctttaatgac agctgctgca tctgcactgc cgccggctag
    12121 accggctgct acaggtatat ttttatcgat atgaatatta acaccttttt ggattttagt
    12181 cttttgcatc actgcttttg ccgcctgaga ggctaagtta ctttcatctg tcggaatctc
    12241 tttactatct gtgataatat taatcccttt tttagaagga acaagggtaa tattatcatg
    12301 taatttgata gactgcatta ccatttctaa attatgaaat ccgtcttctc tgacattttt
    12361 aatatctaaa aataaattga tttttccata agccggtatt ttgactttct ccatgtaaac
    12421 accaccataa tgttgtcaac gccatcacta ttataacacc tctaattaat aaatacaatt
    12481 tattaattag actccatttt acttaaaata gccaggtatt cttctttcga atagggcggt
    12541 ttatttaact gttttaaaac cttttcctta tcaattaaga tatcaattat tgtcttcgct
    12601 aaagctttag ccggttttat aatagccaat tcataatcct ctgttttaaa gtggcgggtg
    12661 tgtaaaccac cgcttacccc ggaaaaaaag gggtgtaaga ccggcatgat atgagaaagg
    12721 tctcccatat caaaagaacc tgtaaatgtt ttctgtttaa ttactctatc tttaggaact
    12781 aaaccttcta aattattagt gaaaatctcc attaatgttt catagttaat taatggtaaa
    12841 taaccgggaa tatccttaat ttcaacttct ccgccaattg catctgaacc ggcttgaata
    12901 gctctattta ctttcttgtt agcatcaaag acagagtcaa cagtactacc ccttacataa
    12961 ctttccatct ttacatcact cggtacgaca ttcaccacat ctccaccctt agttattacg
    13021 ggatgaactc taattttatc actgtcttta aaggtttcac gctgagcatt tatattattt
    13081 atagctaaaa ccgctgcatt taaggcgttt ataccttctt ccggggcacc cccggcatgt
    13141 gattcctcac ctatgaattt aattttttta ccaataaaac catttccaga cggccctact
    13201 attacatctt tcatgtcacc aacatctaag gagtgaacca acatggctat atctatatca
    13261 tcaaagtaac ctcttttgat taattcctgt ttgccgccca aataactaag ctgaccttct
    13321 tcaatcagcc tatctcgata ttcgatctcg ataaattctt cggcgggaac tgccataaaa
    13381 tcaatttggc cctctaattc atttaagatg ttagtcttaa ctaaaccaaa agcaactccc
    13441 agcataatac caatttgatt gttatgacca caagcatgta cggctcctgt ttcagaatca
    13501 gcatccggat ggtctctaca aatcagggca tctaattccc ctaagacagc caggtgaggt
    13561 tcattgtctg tctcaccaat tgatgtttta acccctgtta ctgcgatatt ttcttccggt
    13621 tttaatttta aagtttggaa agcatttttt actaaagctg tggcctcttt ttctttataa
    13681 ccggtctcag ggttattata tatttgttgg actatatcaa taatttcatt tttattttct
    13741 tctattttct tgcaaacctt atttttcaac ttatcccgtc tcatatttat acctcttccc
    13801 caaaagaaaa agaagggatc tttcccttct ctattgtttt tttattcttc ttaaattaaa
    13861 aaatgctgtt aatttattat atcaactcaa agtaatattt tcatgtaaaa tcaaaataaa
    13921 tttgctgtaa tcttagcaga aatttagata aacataaaaa atattatttg aataaagtac
    13981 taattagata taaccctaca actccaattc ctattgtaat agcataggtt gacttttctt
    14041 tttccataaa catcctggta tcccaccagg tttttgggag tttaactatt tcattggctt
    14101 tccatttttc tttacggcca attgcaaaaa catatttgcc tttttcctta ttttcatcga
    14161 aaactgctag ataataggtg ccgtcagtgg gggc
//
LOCUS       DATGCJ010000213         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_208035, whole genome shotgun sequence.
ACCESSION   DATGCJ010000213 DATGCJ010000000
VERSION     DATGCJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208035"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1084
                     /gene="dusB"
                     /locus_tag="VKN64_09680"
     CDS             116..1084
                     /gene="dusB"
                     /locus_tag="VKN64_09680"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMA60551.1"
                     /translation="MEIAGKQINPPIAIAPMAGVTDYPYRQILREMGAELIYTEMVSS
                     KGLVYGSKRTEELLDYDRYSDGYINVQVFGEEPEFIVKASNKIEEEYQPDFIDLNMGC
                     PAPKVVKNGAGSALMKTPSLVKKIVTSMVENLKTPVTVKIRAGWDEDNINGVEIARIA
                     EEAGAQLVAVHGRTREQYYSGQVDYEIIKQVKEAVNIPVIGNGDIFDLESAEKMFAKT
                     SCDGIMVARGIQGNPWLVKRLLKYLGEGIKIPAPSPPEKIEMALYHLEKSVNYYGEEV
                     AIPKMRKHVSWYIKGLKNSTYVKDEINKLKKKEDVIETLKKYKKTF"
     gene            1181..1699
                     /locus_tag="VKN64_09685"
     CDS             1181..1699
                     /locus_tag="VKN64_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMA60552.1"
                     /translation="MKFYRIGDKVINSKYLDKTISKILKLRQSGLSQSKVAEELNIER
                     SFISRLESIGEIRRGNKIALVGFPVENKEEIIEIAKENGLEYTLVMTEEERWDFIQEK
                     SGLEIFEDVIEILVELKEYDLTIFLGSDMRLDLVDKLLDGKVIGIKLGESPIKEDKYV
                     DPTQIKKILNSF"
     gene            1736..>2416
                     /locus_tag="VKN64_09690"
     CDS             1736..>2416
                     /locus_tag="VKN64_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HMA60553.1"
                     /translation="MKKVISISLGSDKRDHKVETEFLGEKFIVERRGTNGDKKKAAQL
                     FTELDGKYDAFGMGGIDLSIQVANNEYYFRDAKKLIKDVKKTPVVDGSGLKNTLERIA
                     VNYIEDNLDIKLHKQRVLLTAALDRFGMAEAFVDKKADLVCGDLIFGLGLPIRIHSLK
                     ALKIIAAIIAPIVTILPFELVYPTGSKQDKGKETDDKFKKFYDEADIIAGDFHYIKAH
                     LPNRIDNKI"
BASE COUNT          895 a          306 c          512 g          703 t
ORIGIN      
        1 attcagaatt aattggcagc aagactgatg caatagaata tattgcccct tttcttactc
       61 ttgatggtct taattactta gcagaaataa atgatttaaa acaggtgagt aaaaaatgga
      121 gattgcagga aaacaaataa atccacccat agcaattgcc cctatggccg gtgtaacaga
      181 ttatccttat agacaaattt tacgggaaat gggagctgaa ttgatctaca cagagatggt
      241 ttctagtaag ggcttagtct atggaagtaa aagaacggaa gagctattag attatgatag
      301 atatagtgat ggatatatta atgtgcaggt ttttggggaa gaaccagaat ttattgttaa
      361 agcttctaac aaaatagaag aagaatatca accagatttt attgacttaa atatgggctg
      421 cccggccccc aaggtagtaa aaaatggggc aggctctgct ttaatgaaaa caccatcttt
      481 agttaaaaaa attgtaacgt ctatggtaga aaatttaaag acccctgtta ctgtaaaaat
      541 tcgggctggc tgggatgaag ataatatcaa tggggttgag atcgcccgga ttgctgaaga
      601 ggcaggagcc cagttagttg ctgttcatgg cagaacaaga gagcagtatt attctggcca
      661 ggtagattat gaaataataa agcaggttaa agaagctgtg aacattccag ttatcggtaa
      721 tggggatatc tttgatttag aatcggcaga gaagatgttt gctaaaacaa gttgtgatgg
      781 tattatggtt gcacggggaa tccagggtaa tccctggctg gtcaagagat tattaaaata
      841 tttaggagaa ggtataaaaa tacccgcacc atcccctcca gaaaaaatag agatggcact
      901 ataccatctg gaaaaatccg tgaattatta tggagaagaa gttgccattc ccaagatgag
      961 aaagcatgta agttggtata ttaaagggtt aaaaaattca acttatgtta aagatgagat
     1021 taacaagtta aaaaagaaag aagatgtcat agagactctc aaaaagtaca aaaaaacttt
     1081 ttaaattaag tctctttagg ggctattttt tttgcattta tatatataaa atgttaaaat
     1141 atgtatgtgc acaaaaatgt gtacagaggg gagatctaag atgaaatttt ataggatcgg
     1201 ggataaagtt atcaacagta aatatttaga taaaactatt agcaaaatac ttaaattacg
     1261 ccaatccggc ctttctcaat ctaaggtagc tgaagaactg aatattgaac gttcctttat
     1321 ctcgcgatta gaaagtattg gagaaataag aagaggcaat aaaattgctc tagtgggttt
     1381 tcctgtcgaa aacaaagaag aaattattga aatagccaaa gaaaatggtt tagaatatac
     1441 acttgttatg acagaagaag aaagatggga ttttattcaa gagaagtccg gactagaaat
     1501 ttttgaggat gttattgaaa ttttagtaga attaaaggag tatgacctaa caatattttt
     1561 aggttcggat atgagattgg atttagtaga taaattatta gatggaaagg ttattgggat
     1621 taagttaggt gaatctccca taaaagaaga taaatatgta gatccaaccc agataaaaaa
     1681 gatattaaat tctttttaaa ctttaaattt tcacgtaata tgtcaggagg tcactatgaa
     1741 aaaagttatt agcatcagtt tgggttcaga taaaagggat cataaagttg aaactgaatt
     1801 tttaggagaa aaatttatcg ttgaaagaag gggcactaat ggtgataaaa agaaagctgc
     1861 ccaacttttt acggaactag atggaaagta tgatgctttt gggatgggtg gtattgatct
     1921 ttccatccag gtggcaaata atgaatatta tttcagagat gctaaaaaat taatcaaaga
     1981 tgtgaaaaaa actcctgtcg tggacggttc tggtttaaaa aacacattgg aaagaattgc
     2041 tgttaactat atcgaagata acttagatat aaaattacat aagcaaagag tattgttaac
     2101 tgcagctttg gatagatttg gtatggcgga agcatttgtt gacaaaaagg cagatttagt
     2161 gtgtggtgac ttaatatttg gacttggact tccgattaga atacattccc tgaaagcctt
     2221 aaaaattata gccgctataa ttgctccgat agtaactata cttccttttg aattagttta
     2281 tccgacaggc agtaagcagg ataaaggaaa agaaacagac gacaaattca aaaagtttta
     2341 tgatgaggcc gatataatag cgggtgattt tcattatatt aaagcacatt taccaaatcg
     2401 gattgataat aaaatt
//
LOCUS       DATGCJ010000214         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_298671, whole genome shotgun sequence.
ACCESSION   DATGCJ010000214 DATGCJ010000000
VERSION     DATGCJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298671"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..574
                     /locus_tag="VKN64_09695"
     CDS             158..574
                     /locus_tag="VKN64_09695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60554.1"
                     /translation="MEIREMKIKDYQKVYSLWEETDIILKKSDEKQEIDRMLKRNPYT
                     CLVGLRDDEIVSVVMGGFDGRRGYVHHLAVKPELQGQGLGREMMDELMNRFEELKVIK
                     VHLFVEENNKDVKAFYRNIGFEERTDLTDFSKTLIK"
     gene            611..1606
                     /locus_tag="VKN64_09700"
     CDS             611..1606
                     /locus_tag="VKN64_09700"
                     /inference="COORDINATES: protein motif:HMM:NF042598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6765 family protein"
                     /protein_id="HMA60555.1"
                     /translation="MDIEFHYYVTYLTALKAGFKREKAEIIATSAQLVDDNVEINLIE
                     NQKGEIYKTYISQTANILKPKEELFRIYPIFHFIPGDPFAFSAQRKDGIMHLLNTTPD
                     NDNARFLMERALDSNNLYRIGIAAHAYTDTWAHQNFTGYYSSFNGLSGVLEKVTPNIG
                     HADAKHNPDLISNIWVDERLISAHQVVDNNSRFIEAVSHLLDYLADYNKNQIKDKKTF
                     LDRIKRIFSIKGNKSYHRFKRINEYNKLSKRLSDYEIPEYEKYKWFESSVDVFNDENI
                     FLPNLNYNEISYKWKDGEHKKSPYYLFCEAIKGHQLAGEKIFEQEKYKGLELNNW"
     gene            1864..>2382
                     /locus_tag="VKN64_09705"
     CDS             1864..>2382
                     /locus_tag="VKN64_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMA60556.1"
                     /translation="MKDIAERAGVSVATVSRVLNNSKPVSEELRDKVLKVVKETGYKP
                     NALARSLIKQRTGTIGVIIPDLDNETFADLIKGIELILDENDYVLVVTNTMGQVEKEL
                     EMFHLFEEKRVDGIIFSGVSLTEEHKEFFSNFEIPTVVVGQEFKELNFPSVTIDNFQA
                     AFDMTQYLIDLGH"
BASE COUNT          895 a          321 c          454 g          712 t
ORIGIN      
        1 ttatagaggt aaagagatgg ttgaggcaag agttacaata gctttagaat tatttgataa
       61 ggcaataaaa ggtgaaaaaa ttaaagaaaa accaatgctc tggaatctca gatttttaag
      121 atacttcttt taaatatatt atacgaggtg ttaatagatg gaaataagag aaatgaagat
      181 aaaagactat caaaaagtat acagcctatg ggaagaaact gatataatat taaaaaaatc
      241 ggatgaaaag caggagatag accgaatgtt aaaaagaaat ccctatactt gcctagtagg
      301 cttaagagat gatgaaatag taagtgttgt aatgggtggt tttgacggac ggcggggata
      361 tgttcatcac ctggcagtta agcccgaact acaggggcag gggctgggaa gagaaatgat
      421 ggatgaatta atgaataggt ttgaggaact caaggttatc aaggttcact tatttgtgga
      481 agagaataat aaagatgtca aagcttttta tcgaaacata ggatttgaag agagaaccga
      541 cctaactgat ttcagtaaga cattaatcaa ataatgagat agattaagca atagcttagg
      601 aggataaaca ttggatattg agtttcatta ttatgtaact tatttaacag ctttaaaagc
      661 aggttttaaa agagaaaagg cagaaattat tgcaacttct gcccaacttg ttgatgacaa
      721 tgtagaaatt aatttaatag aaaaccaaaa gggagaaata tataagacct atataagtca
      781 gactgctaat atcttaaaac caaaagaaga actattcagg atctatccga tttttcattt
      841 tataccggga gatccttttg ctttttcagc ccaaagaaaa gatggcatta tgcatctttt
      901 aaatacaact cctgataatg ataacgcaag gtttttaatg gaaagagctt tagattcaaa
      961 taacttatat cgtatcggga ttgcggctca tgcctataca gatacctggg cccaccagaa
     1021 cttcaccggt tattattcat ctttcaatgg attgagcgga gttttagaaa aggtaactcc
     1081 taatatcgga catgcagatg ccaaacataa tcctgacctt atttcaaata tctgggttga
     1141 tgaaagattg atttcagccc atcaggtagt agataataat tcaaggttta ttgaagcagt
     1201 atcgcattta ctagattact tggctgatta taacaaaaat caaatcaaag acaaaaaaac
     1261 ctttttagat agaattaaaa ggatattttc tattaaggga aacaaaagtt atcacaggtt
     1321 taaaaggatt aatgagtata ataaattaag caaaagatta tctgattatg aaattcctga
     1381 atacgaaaag tacaaatggt ttgaatcttc tgtagacgta tttaatgatg aaaatatatt
     1441 tttacccaat ttaaattaca atgaaataag ttataaatgg aaagatggag agcacaagaa
     1501 atccccctat tacctattct gtgaggctat taaggggcat cagttagcag gagaaaaaat
     1561 atttgagcag gagaaataca agggattaga attgaacaat tggtaaaata agagataaaa
     1621 attttcagaa aatacttgaa atctttccac cattttttta taatataaat gtaaacgttt
     1681 tcctaaaagc taaaaattca tcatgagttt ttacaatttt cttctctaaa accaccaaaa
     1741 ttcttatata ttgacgccaa atcacctttt tacccacaaa ttatacttta tgtaatatat
     1801 tctttagatg taaacgctta ccttttaagc gaaaggggta ttattatggt ccggggttta
     1861 actatgaagg acatagcaga gagagctgga gtttctgtag caacggtatc ccgggttcta
     1921 aataatagca aacccgttag tgaggaatta agggacaaag ttttaaaagt tgtgaaagaa
     1981 accggatata agccaaatgc tctagctcgg agtttaataa aacagagaac cggaacaatt
     2041 ggagtaatta tcccagattt agataatgaa acctttgccg acctcattaa aggtattgaa
     2101 cttattttag atgaaaatga ttatgtatta gtagtaacca atactatggg gcaggtagag
     2161 aaagagctcg aaatgtttca tctttttgaa gaaaaaagag ttgatgggat aattttttca
     2221 ggtgtttcct taaccgagga gcataaagaa tttttttcca actttgagat tccaaccgta
     2281 gtagttggcc aagaatttaa agaattgaac tttccttccg taactattga taattttcaa
     2341 gctgcttttg atatgacaca gtatttaata gatttagggc at
//
LOCUS       DATGCJ010000215         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_165200, whole genome shotgun sequence.
ACCESSION   DATGCJ010000215 DATGCJ010000000
VERSION     DATGCJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165200"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..560
                     /locus_tag="VKN64_09710"
     CDS             294..560
                     /locus_tag="VKN64_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMA60557.1"
                     /translation="MPRIEPSSELRNKYNEIAQFCREYNEPVYITKNGKGDLAVMSIE
                     TFERLVGKYELHKLLDEGINAMKKGNVNSFSKVVGDVKRNLKNE"
     gene            553..867
                     /locus_tag="VKN64_09715"
     CDS             553..867
                     /locus_tag="VKN64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMA60558.1"
                     /translation="MNRYDIEITEPAEKDLYEIGRYIAKELLEQDKAVDVVDKIANNI
                     YKLEEMPFRNAIVDDDKLASQGIRKFIVDNYIVFYNVNEENKVITIIRILYNRRDWLN
                     IL"
     gene            complement(1017..2000)
                     /locus_tag="VKN64_09720"
     CDS             complement(1017..2000)
                     /locus_tag="VKN64_09720"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60559.1"
                     /translation="MIIKSKKIILLVGIFVLTSLIILSGCDTAVKEHTLSIIVNSESA
                     GTVIGAGNYTTGTEIDISALTNEGFRFKNWTVNGEEIETESSFKYSMPDSNVTLTANF
                     YQELSAEDAWNNAEALESNLINDLGNTYYLLLDLAPWISFPQTPDWLQETVVLYSETG
                     STTDFEKTAKSNMGQSVAQLDYKGKTDEKQFFWSGEGEFTINEELNEQGELIKVEIEN
                     DDSFIEVNWTIENKLELQINDSNDSLLKNIDWADGQAAESSLIELANYLNEIDWDPRK
                     IVPEADRSHIMLTVGAMDGLREGPILGIKEPYKSIIKNILTGGVGTLINHI"
     gene            2611..3300
                     /locus_tag="VKN64_09725"
     CDS             2611..3300
                     /locus_tag="VKN64_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60560.1"
                     /translation="MKGLSAKLKEEIDNVFEVLAYVQAVNMNNYSGNEEILSDGLRWE
                     ISAHPEYVIENNEANCFAMANTVAYLLEGDYEEVGVVWWSIPHYGGHLYNYVKYEGEY
                     YIFSALAYTMPSDSTPGVDGYPGYENGADNGYTGNLRGLVHQILEDENGNPNFDPWFK
                     IEKDFHENLTFMAIIPTTGFAVIGSHGEDENAYHVATDFPLDKTIIVRETPVYLTKNQ
                     KAPEWKRGLYE"
BASE COUNT         1228 a          552 c          616 g         1285 t
ORIGIN      
        1 taataaaaca aaagaaaaaa gataagaaga tcgagataaa aacagaaaga atatttaaaa
       61 gaagtaaaac taagaatcta tatcctgaag ccaaagaagt actgccagca ttaaaaagtg
      121 ggaaagttaa tggaacatat aaagcaataa aggcttatgc cttttaaata aatctgaaag
      181 agagtttatc ttaaatccaa aagtaacttg acactatcac cataagataa atcgttgatt
      241 taatatcaat attatgatag aatatatgta taataaaaat ggaggtgcta tttatgccca
      301 gaatcgaacc aagttctgaa ctgagaaata aatataatga aattgctcaa ttttgtcgtg
      361 aatataatga gcctgtttat attactaaaa acggcaaagg agatttagct gtaatgagta
      421 ttgagacttt tgaacgtctt gttggaaaat atgaactcca taaattatta gatgaaggta
      481 taaatgcaat gaaaaaaggt aatgttaact ctttttcgaa agtagtaggt gacgtaaaaa
      541 ggaatctaaa aaatgaatag atatgatatt gaaattactg aaccagcaga aaaagattta
      601 tatgaaatag ggagatatat tgctaaggaa ttgttagagc aggataaggc agtagatgta
      661 gttgacaaaa tagctaataa tatatataaa ttagaagaga tgccgtttag aaatgctatt
      721 gttgatgatg ataaactagc atctcaaggc atacgaaaat ttattgtaga taattatatt
      781 gtgttttata atgttaatga agaaaataag gttataacga ttattaggat tctttataat
      841 agaagggact ggcttaatat attataatta cgttaaggta ccgggtacct cacgctaaga
      901 gtaatccttg attttttgtt gattagatat aacccatctt agcttcctaa aaagtagtac
      961 attttaataa aaaagcccgg cataaccggg cagaaataaa tttaatttat attattttat
     1021 atgtggttaa tcagtgtacc tactccaccc gttaaaatgt tttttataat actcttatat
     1081 ggttctttta ttcccaggat cggtccttcc cgcaatccat ccatagcacc aacagtcagc
     1141 attatatggc ttcggtctgc ttctggaact atttttcttg ggtcccagtc tatttcatta
     1201 agataattgg ctaattcaat taaactactt tcagcagcct gtccgtctgc ccagtctata
     1261 ttcttaagta acgaatcatt actatcgtta atttgaagct ccaatttatt ttctatagtc
     1321 caatttactt ctataaagct atcatcattt tctatctcaa ccttaattag ctctccttgt
     1381 tcattaagtt cctcattgat agtaaattca ccttctcctg accagaaaaa ttgtttttca
     1441 tctgtttttc ctttataatc cagttgagct actgattgtc ccatgttaga ctttgctgtt
     1501 ttttcaaaat ctgttgtact gccggtttca ctatataata ctactgtctc ttgcagccag
     1561 tcaggggtct gtgggaaaga tatccaagga gccagatcca gaagcaagta ataagtatta
     1621 ccgaggtcat taatcagatt actttccagg gcttcggcat tattccaggc atcttctgca
     1681 gatagttcct ggtaaaaatt ggctgttaat gtcacattag aatcaggcat ggagtattta
     1741 aaagaagact ctgtctcaat ttcctctcca ttaactgtcc agtttttaaa tctaaatcct
     1801 tcatttgtca gtgcactaat gtcaatttcc gttcctgtcg tataatttcc tgctccaatt
     1861 acggtacctg cactttctga atttactatg atactaagtg tatgttcctt tacagcagtg
     1921 tcacagccgc ttaatattat cagacttgtc aacacaaata tacccactaa caatataatt
     1981 ttcttacttt ttataatcat gaatatgctc ccttctaatt ttgaagtagt gagattgtta
     2041 gaatccagct ataacactac atgttgtttt atcacctcct attgtttttt tgacctcatg
     2101 taatatttag acaggtatta tttattttcc aaattatttt tatgtttttt taaatgtttt
     2161 acaaaatatt tttaatattt ataatagtaa tattcattat caatttcata aatcctgcag
     2221 aaaatttata ctgaattact tcccgacaac taagagtatt ataattacgc taaggtaccg
     2281 ggtatttccc tgtatatttc gaaaaattta atgtgcaagt atttaagata agattaaccc
     2341 tcaaactgac ttacagttgg tttagggttt ttttgtataa gtataattta ggttatcttt
     2401 ttcagggtag atataaatct aaagtaatag aagatgatgc ttataatctg tctactgtgt
     2461 ttttccaatg cttattaatc attgtcgtaa aatgtttatt tattttttag aattgtttca
     2521 ggaacttggt caaatgatta tggcaaaata tattgggtac catctttttc tttgggaaaa
     2581 cctgatacaa cagtaaaaga gctcatagaa atgaaaggac tgtctgcaaa attaaaagaa
     2641 gagatagata atgtttttga ggtattagcc tatgtacagg ctgttaatat gaataactac
     2701 agtggtaatg aagaaatatt atcggatgga ttaaggtggg aaatatcggc tcatccggaa
     2761 tatgtaatag aaaataatga agctaactgt tttgccatgg ctaatacagt tgcctatcta
     2821 cttgaagggg attatgaaga ggtgggtgtt gtctggtggt ctattccgca ttatggtggt
     2881 catctttata attatgtaaa gtatgagggg gaatattaca tttttagtgc tttagcttat
     2941 acaatgccat ctgattctac accaggtgtt gatggctatc ctggatacga aaacggggct
     3001 gacaatggct atactggtaa cttaagaggg cttgtccacc agattttaga agatgaaaat
     3061 ggtaatccta attttgatcc ctggttcaag atagagaaag actttcatga aaacctaact
     3121 tttatggcca taattcctac aacaggtttt gcagtgatag gttcacatgg agaagatgaa
     3181 aatgcttacc atgttgctac agactttcca ttagataaaa ctataattgt tcgtgagact
     3241 cctgtctatt tgaccaaaaa ccaaaaagca cctgagtgga agagaggtct ctatgaatag
     3301 tagtttaggg taccgggtac ataccggaaa ttgccttttg gagagaatag gagaaagcta
     3361 ggttttaaat taagtaaacc acagcagaga tttttactct actgtggttt aaatttcgat
     3421 tttattatta aaatttaatt attgactata ctgcgaaaaa tggattgttg aacccatcct
     3481 aagcaaaatc tgatctgatt aataatcata tatttgttga tttggatatt ttacttactt
     3541 ataataattt ttatttcgaa ctatcattat ttaaactata tatatcctat ttattgctta
     3601 ttaagtcctt atataacctc taatgtttcc atttaatatt ctaactaatt gttttatgtt
     3661 atatccgagc gctttataca t
//
LOCUS       DATGCJ010000216         8479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_611066, whole genome shotgun sequence.
ACCESSION   DATGCJ010000216 DATGCJ010000000
VERSION     DATGCJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611066"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /gene="meaB"
                     /locus_tag="VKN64_09730"
     CDS             <1..809
                     /gene="meaB"
                     /locus_tag="VKN64_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMA60561.1"
                     /translation="AYMVGISGPPGSGKSTLTQRIVQLWREKDLKIGIIAIDPTSHFS
                     GGALLGDRIRMNEIATDKGVFIRSMATRGSLGGLNSSIYDTVLIFSLAGFDYIIIETV
                     GVGQNEIDIVKLADTTIFVTVPEAGDEIQAYKAGLMEVGDIFVVNKADRPKSHQMVLT
                     IEQLVSFKEGKDVWQPPVLSTNALKNEGIADINEWINKHHEYLKEKNLMQANRKNVIR
                     TQMINLMEKDLHEKFIKPILNDEKFEEFIESVYRKDKDPYTAVKKLLSFD"
     gene            832..1227
                     /gene="mce"
                     /locus_tag="VKN64_09735"
     CDS             832..1227
                     /gene="mce"
                     /locus_tag="VKN64_09735"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMA60562.1"
                     /translation="MKIDHVGIAVRNIDESLTFYRDILGLEFEGIEVLEDRGLKTAFL
                     KTGESEIELLEPISEDSTIYKFLEKKGEGIHHLALEVDDVEEKLESMSEKGANFIGEA
                     SIGAGGKKIIFIHPKSTSGVLMELCQHMD"
     gene            1503..2768
                     /locus_tag="VKN64_09740"
     CDS             1503..2768
                     /locus_tag="VKN64_09740"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMA60563.1"
                     /translation="MSKDTYNPYQEFIDTIDEAGQNLGLPRDDYKILEKPDRELKVSI
                     PIEKDDGSVEVFDGYRVQHSNSRGPYKGGVRYHPDVNIDEIRALAGWMTLKCSLVDIP
                     YGGAKGGIQCNPRELSQSELKKITRRYTTMIEPVIGIDKDIPAPDVNTNAQIMGWIYD
                     TYSMIKGHNIPGIVTGKPRIIEGCLAREGATGRGVMITVVNLFKKLGKNLNGTKVAIQ
                     GFGNVGQVAAELLQEKGCKIVAVSDVSGGLYNEEGLDIVKLREFVGSDRKHLAEYEKE
                     ENTKDITNAELLRSDVDVLIPAALENQITAEVARDVKAKYIVEAANGPTTREADQILA
                     EREVVVMPDILSNAGGVIVSYFEWVQNKESSRWEDSKTNRKLRNLLTDAFEQVWETYE
                     DKQVSFREAAYMVALNRIVETKKIRGIFP"
     gene            2857..4122
                     /locus_tag="VKN64_09745"
     CDS             2857..4122
                     /locus_tag="VKN64_09745"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMA60564.1"
                     /translation="MGNSTVYENVLKQLDNAAEVLDLDEQIRELLQEPMRTLSVSIPV
                     KLDDGNIHVFKGFRCQHNNVLGPTKGGIRFHPQVSEDEVKALAAWMTFKCSLVGIPYG
                     GAKGGVIVNPKELSRNELERLSRGFIQSISPIIGPDKDIPAPDVYTDAQVMSWFMDEF
                     SKIKGVNTPGLVTGKPVVLGGSFGRHSATARGVMYTVREAAKAIDLDLKGATVAVQGY
                     GNAGSFSAKFLNELGCKIVAANDSKGGIYCEDGFDPVEVAKHKDETGSVKGFEGCEEI
                     SNQELLTMDVDVLVPAALENQITKDNANDIKARMIAEAANGPTTPEADEILFQKEIMI
                     IPDILANAGGVTVSYFEWVQNLANYYWSKEEVDRKLEDIMVDAFEKVFNTHEELGVNM
                     RVSAFIVAIKRLTEAMEARGWVYNGDKNC"
     gene            4254..5582
                     /locus_tag="VKN64_09750"
     CDS             4254..5582
                     /locus_tag="VKN64_09750"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HMA60565.1"
                     /translation="MTDVKLTETILRDGHQSLLATRMETDEMLPVLEEMDKLGYHSME
                     VWGGATFDSCIRYLNEDPWERLRKFKQKAKNTPLQMLLRGQNILGYKHYPDDVLEKFV
                     EKSIKNGIDIIRIFDALNDVRNLKRAIEFTNKYGGHAQGTIVYTTSPLHDIEHYIETA
                     KEVERMGADSLCLKDMAGLLAPFEAYNLIKKLKEEINIPIQLHAHNTTGMAAMTYLKA
                     VEAGVDVIDTALSALGSGSSQPSTEPFIYVLQNSQYDTGIDFDKANEINEHFRAIRDS
                     KKEFLGSFGVDPRVLKSQIPGGMLSNLRSQLKDQNMFDKYDEVLKEVPKVRAEMGYPP
                     LVTPTSQIIGIQAVFNVATGDRYSVVSKEIKNYIKGQYGTPPGPIDEDLKEQILKGES
                     VIEGRYADQLEPALDKYREEIKEYMQQEEDVLTYALFPSVAEKFFEKRIS"
     gene            5604..7454
                     /locus_tag="VKN64_09755"
     CDS             5604..7454
                     /locus_tag="VKN64_09755"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HMA60566.1"
                     /translation="MDLSAVNQEKLKNLNNEKVEDLLEFAVDLCKPDKISIIDDSEED
                     IQYVRDLAIENGEEKPLDMEGHTVHFDGYNDQARDKHNTRYLVEEELDLGLDINTIER
                     EEGLDEIFSYLDGSMEGKEMLVRFFSLGPNQSDFSIPAMQITDSSYVAHSEDILYRQG
                     YEDFKNLNGSKDFFFFLHSAGRLENGVSADIDKRRIYIDLKTDRVFSVNNQYAGNSLG
                     LKKLAFRLAIRRAVREGWMAEHMFISGVNGPADRKTYFTGAFPSSCGKTSTAMIPGHT
                     IVGDDIAYLKLKGKDVRAVNVENGAFGIIRDVNPEDDPVIYNTLTTPRELIFSNVLIN
                     DGKPYWLGMGKEIPEEGINFSGEWHKGKTDEEGNKIPPAHKNARYTIRISDLENCDEN
                     FDKKEGVPIKGMVFGGRDSDTSVPVAETLNWTHGVFMASSLESETTSATLDKQGERKH
                     NPMANLDFLSVPISDYINNYLQFGQKAKNQPSIFTVNYFLKDPETGEYLNGMLDKKIW
                     MLWMEKRVHGEVKAIKSPVGYLPYYEDLRQLFARYLSFDYTEIGYVEQFSIRVENYLA
                     KFNRIKKAFKDIPNLPDEFEKELETQIERLKAARKKYSDSEISPFKLKNY"
     gene            7470..>8479
                     /locus_tag="VKN64_09760"
     CDS             7470..>8479
                     /locus_tag="VKN64_09760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMA60567.1"
                     /translation="MELSKRAQKISASLTLAISAKANKLKAAGEDVVSFGAGEPDFDT
                     PEYICEAAREALEVGFTRYTAASGYEDLKNAVKDKLKRDNNLEYDTDQIIVSNGAKHS
                     LFNTFQAILNPGDEVIIPVPYWLTYPETVKMGGGKPVFIETKEEEGFKINIERLQEMV
                     NEKTKALILNSPSNPTGSIYNKEELEAIAKVAVDNEIIVVSDEIYEKIVYDGNEHISI
                     ASLNEDIKDLTIVINGVSKAYAMTGWRIGYAAGPQEIIEIMSNIQSHATSNPNSIAQY
                     ASNAGLRGDHSFMEERRNKFSERRDYMYDKINSIEGLSCLKPGGAFYIMMNIEDIIGK
                     NIA"
BASE COUNT         2926 a         1287 c         1872 g         2394 t
ORIGIN      
        1 ctgcatacat ggtgggaatt tctggtccac ctggttccgg taaaagcact ttaactcagc
       61 ggattgttca gctctggcgt gaaaaagatt taaagatcgg tataattgct attgatccca
      121 ccagccattt tagtggtggg gcactcttag gggacagaat taggatgaat gaaattgcta
      181 ctgataaagg tgtctttatt aggagtatgg ccaccagggg ttctttgggt ggacttaatt
      241 cttctatcta tgataccgta ttaatttttt ccctggccgg ttttgattac attataatcg
      301 aaacggtggg agttggacaa aacgagatag atatagtaaa attagctgat acaacaattt
      361 ttgtgacagt accggaagcc ggtgatgaga tccaggctta taaagccggt ttgatggaag
      421 ttggggatat ctttgtagtc aacaaggcag atcgccccaa atcccatcag atggttttaa
      481 ctatcgaaca actggtttca tttaaagaag gcaaagatgt ctggcagcca cctgttttat
      541 caaccaatgc tttaaaaaat gaaggaatag ccgatattaa tgagtggatt aataaacatc
      601 atgagtattt aaaggaaaaa aatttaatgc aagcaaaccg taaaaatgtt ataaggaccc
      661 agatgattaa tttaatggaa aaagatttac atgaaaagtt cattaaacca atcctaaatg
      721 atgaaaagtt tgaagaattt attgaatccg tgtatagaaa agacaaggat ccttatactg
      781 cggttaaaaa attactgtct tttgattagt tagaaaggaa ggtacatata tatgaaaata
      841 gatcatgttg gtatagctgt aagaaatatt gatgagtctt taacctttta cagggacatt
      901 ttaggtctag aatttgaagg aatagaagtt ttagaggata gaggattaaa gaccgctttt
      961 ttaaagacag gtgaaagtga gatagagtta ttagaaccaa tttcagagga cagtactata
     1021 tataaatttt tagagaagaa gggagaaggt atccatcacc tggctctaga agtagatgat
     1081 gtagaagaga agttagagag tatgagtgaa aaaggggcta attttatagg agaagcaagt
     1141 attggagcag gcggtaaaaa aataattttt atacatccca agtcaaccag tggagttctg
     1201 atggaactct gtcagcatat ggattaattt aataagaaag tagttaaatt aaaaaagtaa
     1261 tgctttaagt cagattttag atgtcataat ttttaattaa tttaaaaaaa tttatgaagt
     1321 aaatattagg atgtatgaaa ataagggtta tgaaaggagg cacgatcaac aaaaatataa
     1381 aaaattttgt gctcacaata agcttgcttt attgattttt cttagatttt aaatagattt
     1441 aaaataaaaa aatgaagact aaaaaaagaa atatagtctt atataatagg agtgaattaa
     1501 aattgtctaa agatacatat aatccttatc aagaatttat agatacgata gacgaggcag
     1561 gtcaaaacct gggattaccc cgagatgatt acaagatatt agaaaaacca gaccgtgaat
     1621 taaaagtatc tatccccatt gaaaaagatg atggaagtgt tgaagtcttt gatggataca
     1681 gggtacagca cagtaattca agaggtccct ataaaggtgg agtccgctac catcccgatg
     1741 taaatattga tgaaattagg gctctagcag gttggatgac tttaaaatgc tcactagttg
     1801 atatccctta tggtggtgca aaaggcggta tacagtgtaa cccccgcgaa ctatcccagt
     1861 ctgagttaaa aaagattacc agaagataca ctaccatgat cgaaccggtg attggtatcg
     1921 ataaagatat tccagcccca gatgtaaata cgaatgcgca aattatgggc tggatatatg
     1981 atacttacag tatgattaag ggccacaata taccaggtat tgtaaccggt aaaccgagaa
     2041 ttattgaagg ctgccttgcc cgggaagggg ctaccggccg cggtgtaatg attacagtag
     2101 taaacctctt taagaagtta ggaaagaatc ttaatggtac taaagttgct attcagggct
     2161 ttggtaatgt tggtcaggtc gctgcagaat tactgcaaga aaaaggctgt aagatcgtgg
     2221 cagttagtga tgtttcaggc ggactatata atgaagaagg cttagatatt gtaaaattaa
     2281 gagagtttgt cggctcagac cgtaaacact tggctgaata tgaaaaggaa gaaaacacca
     2341 aagacattac caatgcagaa cttctcaggt cagatgttga tgtattaatc ccggctgctt
     2401 tagaaaatca aattacggct gaagttgccc gtgatgtaaa agctaaatat atagtagaag
     2461 ctgccaatgg acctactacc agagaagctg atcaaatctt agcagaaaga gaagttgttg
     2521 tcatgcctga tattttatca aatgcaggtg gagtaattgt ctcctacttt gaatgggtac
     2581 aaaataaaga atcctcaagg tgggaagact ccaaaacaaa ccgtaagctg cgtaacctct
     2641 taactgatgc tttcgaacag gtttgggaga cctatgaaga taaacaggtt agctttagag
     2701 aagcagccta tatggtagcc ttaaatagaa tcgtagaaac caaaaaaatc agaggcattt
     2761 tcccataaat cgattaaaga taagttaaaa agtagaacag cagagagtaa taattaataa
     2821 atttatttat tcaataattg agtagggggt atgttaatgg gtaattctac tgtttatgaa
     2881 aatgttttaa aacaacttga taatgcagct gaggttttgg atcttgatga acaaatcagg
     2941 gaattattac aggagccaat gaggacatta tcagtttcaa ttccggttaa attagatgat
     3001 ggtaacattc atgtttttaa gggatttaga tgtcagcaca ataatgtgct gggacctacc
     3061 aaaggtggaa ttagatttca tccccaggta agtgaagatg aagtaaaggc cctggcagcc
     3121 tggatgacct ttaaatgttc cctggtcggt attccttatg ggggagctaa agggggcgta
     3181 attgtaaacc ctaaagaact ttctagaaac gaattagaaa ggttaagtag aggcttcatt
     3241 cagtctattt ctcccataat tggacctgat aaagatatcc cggctcctga tgtctataca
     3301 gatgcccagg tgatgagttg gtttatggat gaattttcta aaataaaagg tgtaaatact
     3361 cccggccttg ttacaggtaa accggttgta ctgggtggtt cctttggacg ccactctgct
     3421 actgcccggg gtgtaatgta tacggtacga gaagcagcta aagcaattga tttagacctt
     3481 aaaggggcaa ccgttgccgt ccagggctat ggtaatgccg gtagtttttc cgccaagttc
     3541 ttaaatgaat taggttgtaa gattgttgca gccaatgatt ccaaaggcgg tatttactgt
     3601 gaagacggtt ttgatccggt tgaagtagcc aaacataaag atgaaaccgg cagtgtgaag
     3661 ggatttgaag gttgtgagga aatttcaaat caggaattac tcacaatgga tgtagatgtc
     3721 ttagtaccgg ccgccttaga aaatcagatc acaaaagata atgcaaatga tatcaaggcc
     3781 agaatgattg ctgaagcagc taatggaccg acaactccgg aagctgacga gatattattc
     3841 caaaaggaga tcatgataat tcctgatatc ttagcgaatg ccggtggagt taccgtatct
     3901 tacttcgaat gggtgcaaaa ccttgccaat tattactggt caaaagaaga agtggataga
     3961 aagttagaag atatcatggt tgatgccttt gaaaaggtct ttaataccca tgaagaactc
     4021 ggtgttaata tgagagtttc tgcctttatt gtagccatta aaaggttaac tgaagctatg
     4081 gaagcaagag gctgggtata taacggtgat aaaaattgtt aactttgcat aaatgtataa
     4141 aataggggcc tatttggccc ctttttataa tatctaataa tattttttgc attcatatag
     4201 attttattat aatatcaatt taacattaag aaacagcaag gaggtttaaa ccattgactg
     4261 acgtaaaatt gacagaaacg attttaagag atggacacca atcgctttta gcaacgagga
     4321 tggaaacaga tgaaatgctg cctgtattag aagagatgga taaattaggt taccactcca
     4381 tggaagtctg gggaggagct acctttgaca gctgtataag gtatttaaac gaagatccct
     4441 gggaaaggct tagaaagttc aaacaaaagg caaaaaatac gcctttacag atgctattaa
     4501 ggggacaaaa cattttaggg tacaagcatt atcccgatga tgtcttagaa aaattcgttg
     4561 aaaaatctat taaaaatggc attgatataa taaggatctt cgatgcatta aatgatgtta
     4621 ggaacttaaa aagggctatc gaatttacta acaaatatgg gggtcatgcc cagggaacta
     4681 tcgtatatac aaccagcccg ctccatgata ttgaacatta tattgaaaca gctaaagaag
     4741 tggaaaggat gggtgcagat tcgctctgcc tgaaggatat ggccggactt ttagcacctt
     4801 ttgaagccta taatctgatt aaaaaactaa aagaagaaat taatattccg attcaactac
     4861 atgcccacaa tactacaggt atggctgcaa tgacctatct aaaggctgta gaggccgggg
     4921 tcgatgtaat agataccgca ctttccgctt tgggctcggg tagttcacag ccctcgacag
     4981 aaccatttat ttatgtactg caaaacagcc aatatgatac aggtattgat tttgataagg
     5041 ccaatgaaat aaatgaacat tttagagcca tcagagacag taaaaaggaa tttttaggat
     5101 cttttggagt agacccccga gtattaaaaa gccagattcc gggcggtatg ctctctaatt
     5161 taagaagtca gctaaaagat caaaatatgt tcgataaata tgatgaagta ttaaaggagg
     5221 tacccaaagt aagggcagaa atgggttatc ctcccctcgt cacaccgacc agccaaatta
     5281 ttggtattca ggctgtcttt aatgtggcga ccggtgatag atatagtgtg gtttctaaag
     5341 agattaaaaa ttatatcaag ggccagtacg gtacaccacc tggacctatt gatgaagatt
     5401 taaaagaaca gatattaaaa ggggaaagtg taattgaagg tagatatgcc gatcaactag
     5461 agcctgcttt agataaatat cgagaggaga ttaaagaata tatgcaacag gaagaagatg
     5521 tattaaccta tgcattattt ccctctgtag ctgaaaagtt ttttgaaaaa aggattagtt
     5581 aaaaagtaag gaggagaatt gttttggatt taagtgccgt aaaccaagaa aaattaaaaa
     5641 accttaataa tgaaaaggta gaagatcttt tagaatttgc tgttgatctt tgtaaacctg
     5701 ataaaatttc aatcatagat gacagtgaag aagatatcca gtatgtacgc gatcttgcaa
     5761 tagaaaatgg tgaagaaaaa cctcttgata tggaaggcca taccgttcat tttgatggat
     5821 acaatgatca ggcacgagat aaacataata caagatattt agtggaagaa gaactggatt
     5881 taggattaga tatcaataca attgagcggg aagaagggct agatgagatc ttttcatatc
     5941 tagatggcag tatggaaggg aaagagatgc tggtcaggtt tttctccctg ggacccaatc
     6001 aatctgactt tagtattccc gccatgcaga ttacagattc gagttatgta gcccatagtg
     6061 aagatatttt atatagacag ggttatgaag atttcaagaa tttaaatggt tctaaagatt
     6121 tcttcttttt ccttcattca gccggacgtt tagaaaacgg agttagtgct gatatcgata
     6181 aaagaagaat atatattgat ttaaagaccg atcgggtatt ctctgtgaac aaccaatatg
     6241 ctggtaatag tctggggctt aaaaaactag ccttcagact ggcaatacgc cgggccgtac
     6301 gagaaggctg gatggctgag catatgttta tctcgggtgt taatggacct gctgatagaa
     6361 aaacctactt tacgggggct tttccttcta gttgtgggaa aactagtaca gctatgatcc
     6421 cgggccatac cattgttggt gatgatattg cctatctaaa actaaagggt aaagatgtaa
     6481 gggcggttaa tgtagaaaat ggcgcctttg gtattatcag ggatgttaat cccgaagatg
     6541 atcccgtgat ttataatacc ttaactaccc cgcgtgaact aatcttttcc aatgtgttaa
     6601 ttaatgacgg taagccttat tggctcggta tgggcaaaga aattcccgag gaaggtatta
     6661 acttctcagg tgaatggcat aagggtaaga cagatgaaga gggcaataaa atcccgcctg
     6721 cccataaaaa tgccagatat acgataagaa tcagtgacct tgagaactgt gatgagaatt
     6781 ttgataaaaa agaaggtgtt ccgatcaaag gtatggtttt tggcggccgt gattctgata
     6841 catctgtccc tgtagcagaa acccttaact ggacccatgg agtatttatg gcctcttctt
     6901 tagaatcaga aactacctct gctactttag ataaacaagg ggaaagaaag cataatccga
     6961 tggctaactt ggattttctt tctgtaccga tcagtgatta tattaataat taccttcagt
     7021 tcggacaaaa agctaaaaat caaccgagta tttttacagt caactacttt ttaaaagatc
     7081 ctgaaaccgg tgaatattta aatggaatgc tggataaaaa gatctggatg ctctggatgg
     7141 aaaaaagagt acatggtgaa gttaaggcaa ttaaatctcc tgtcggttac ttaccatatt
     7201 atgaagattt gagacagcta tttgcccgtt atttaagttt tgattatact gagattggtt
     7261 atgtagagca gttttctatt agagtggaaa attatctggc taagtttaat aggattaaaa
     7321 aagcttttaa ggatataccg aatttacctg atgaatttga aaaagaatta gaaactcaaa
     7381 ttgagcggtt gaaagcagcc cgtaagaaat atagtgatag tgagatctca ccatttaagt
     7441 taaaaaatta ttaaaggggg aagattactt tggaactatc aaaaagagct caaaaaatta
     7501 gtgcttcttt aaccctggcg attagtgcaa aggcaaataa gttgaaagca gccggtgaag
     7561 atgtagttag ttttggggcc ggtgaaccgg attttgatac acccgaatat atttgtgaag
     7621 cagctcggga agcattagag gttggcttta ccagatatac cgctgcctcc ggttatgagg
     7681 accttaaaaa tgcggtcaaa gataaattga aaagagataa taatttagaa tatgataccg
     7741 atcaaatcat tgtttcaaat ggagcaaagc attccttatt taataccttt caggccattt
     7801 taaatccggg cgatgaggtt attatcccgg ttccttactg gttaacttac ccggaaacag
     7861 ttaagatggg gggaggtaaa cccgttttca ttgagaccaa ggaagaagaa ggttttaaga
     7921 ttaatataga aagattacaa gagatggtta atgaaaagac aaaggcctta atattaaaca
     7981 gtccgagtaa ccctaccggt agtatttata ataaagaaga attagaagca attgccaaag
     8041 tagctgtgga taatgaaatc atcgtggttt ccgatgaaat atatgaaaag attgtctatg
     8101 atggaaatga acatattagt attgcttctt taaatgaaga tatcaaagat ctaaccattg
     8161 tcattaatgg cgtttcaaaa gcttatgcga tgacgggttg gcgaatcgga tatgcagctg
     8221 gtccccaaga aataattgaa attatgtcta atattcaaag ccatgccacc tcaaatccga
     8281 actcaattgc tcaatatgcc agtaatgcag gcctaagagg cgatcacagc ttcatggaag
     8341 aaagaagaaa caagttctct gaaagaaggg actacatgta tgataagatt aattctattg
     8401 aaggactttc ctgtctaaag ccgggtggtg ctttttatat catgatgaat atagaggata
     8461 taataggcaa aaatattgc
//
LOCUS       DATGCJ010000217         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_389919, whole genome shotgun sequence.
ACCESSION   DATGCJ010000217 DATGCJ010000000
VERSION     DATGCJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389919"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VKN64_09765"
     CDS             <1..368
                     /locus_tag="VKN64_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60568.1"
                     /translation="KWRCPHVCLKSVNCPYFNRPEDDYGRTYYTKFDDDIRLFTPTVR
                     GSKKWENTMNKRTSSERCNDRIKNDYRLQSDKVRSKSRWLLRIIMRDAAMHVNAWVNT
                     AHLSSEKWLSSWFSLEQTA"
     gene            complement(1403..2065)
                     /locus_tag="VKN64_09770"
     CDS             complement(1403..2065)
                     /locus_tag="VKN64_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HMA60569.1"
                     /translation="MSKREKFTPKSLKSGVVDYLKEQIFLENYLGGDRIIESKVADEL
                     DISRAPVREAIKELENKGLVETIPRKGTFVVEFELDEIQELFDIRVMLEERVFKILIE
                     EEKMNEEDFNYLESLVEEMVKITESDMDDNLKIFEVNKRDMKFHSYLWKKANSKWTNK
                     ILTTLYYQLQLAMIIDAKKEENLKEASLKHKDILKYLKKESVKETKKAVIDHIRIFSE
                     MV"
     gene            complement(2681..3916)
                     /locus_tag="VKN64_09775"
     CDS             complement(2681..3916)
                     /locus_tag="VKN64_09775"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMA60570.1"
                     /translation="MDNVFNSALDSLNKAGELAGIDPNVMELMSRPKRIIDFSIPLKM
                     DDGSFKMFDAYRVHYNDALGPTRDGTRFVPNLHIDEVKALALFMTIKHAVSGVPAGGG
                     KGGIKVDPSKLSDWELERLTRGFIRNLKPKGPKIDIPGADIGTDYRTQSWMLDEYEQI
                     TGEHNPPAINDKPNIMGGTVGSAEATGLGIFYVTKEASKDNNIDLAKEEVVIQGFGQV
                     GATIARYLYDIGAKITAVSDISGGFYNKDGLDIPAMQKYCEENNGLEGYSEAESISNE
                     ELLELECGLLIPAAVQNVINGQNADNINTKMIIEAANGPITPEAEAILKDNGIKVIPD
                     VVANAGGAIVCHYERIQGITDDYWDIEKVEKRLNKQILTAYKNARDTAEEFDTTTRLG
                     AWIVALRKVAEAVKMRGWV"
     gene            complement(4058..5011)
                     /locus_tag="VKN64_09780"
     CDS             complement(4058..5011)
                     /locus_tag="VKN64_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60571.1"
                     /translation="MAFKEMAAYIKNDTEYSLRERYDMPDSELTFPGGAHARIEISGI
                     ESASNLEEMVKEADKRNITIHRVISIVRGATMLDDQELKHFAQVGADNDLELLVNPVA
                     SREWDTGRQYTSGEEGIVSGMRLRGHDMLYRYLKNIDRCIEAGIRGFLITDESALTLL
                     NDMRDKGIIPQDVKFKVSVFAGHGTAVAGKMLERLGADSFNPLADMTLPMLSSIRQGG
                     VKVPLDVYMSLVESMGGFQRYDEAADIARIASPVYFKIEPGPSEADLYNSWIDSQSPN
                     YLDNLAKMRVKIAQIIMEWIDRSEHDIVMNDYKEDLSVPQV"
     gene            complement(5050..5883)
                     /gene="panB"
                     /locus_tag="VKN64_09785"
     CDS             complement(5050..5883)
                     /gene="panB"
                     /locus_tag="VKN64_09785"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMA60572.1"
                     /translation="MLDFNEMKQKGENVAWITAYDFPTAQFAEKAGMDMILVGDSLGM
                     VVKGYKGTVPVTMEDCIAHSQDVRRGAPNTYCIGDMPFGSYQVSDEKAVENAARFLKE
                     ADMDAIKLEGGRRVASQIEAISDAGIVVFGHIGLTPQSSGQLGGFKAQGLTVDSAREL
                     IKDAVAVQEAGAQALLLEAVPPEVTEFITDKLDIPVYSIGAGLPCDGQLLICGDMLGM
                     FQAFTPKFVKQYANVAEIEIEAFKEYIDDVKNEKFPEDEHVYHIKDSEEDFEELFSEF
                     E"
     gene            complement(6152..6763)
                     /locus_tag="VKN64_09790"
     CDS             complement(6152..6763)
                     /locus_tag="VKN64_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMA60573.1"
                     /translation="MIFYTYPFITAGISSFFLNMKLTKKVMGAIIVSFIGLTFVLGKT
                     SGDIVLVGLLFAFGAAVFYSVYTLFGDHILKDVPLLTTIAMVCLFAAISFLTVGLISN
                     NVQFAFTSNTWLPVIGIAVVSMLGFLAFFQGIKMTSPTSILVLSMIEPIVTIVLSTIF
                     FNDILNLYQIIGAILVLGGSAMVVLSVKEKKEVACSSNEKKFS"
     gene            complement(7142..>7696)
                     /locus_tag="VKN64_09795"
     CDS             complement(7142..>7696)
                     /locus_tag="VKN64_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA60574.1"
                     /translation="IGTHALDLTLWIMDNYEPVTVSGSVFNKMSDQTKGNLFGPWNPN
                     KYGVEDSAFGFIKMKNGAAVFLESAWILNTRDPKEASATFYGTKRGAELRWNLSDDKN
                     ELIYNMIEEGQYKEIKESSIKKMGPAASEKVSLGYLEAASWINAIIRDKSPLVKPEEA
                     YMVTKILDAVYESERKGREIKFDN"
BASE COUNT         2423 a         1502 c         1012 g         2759 t
ORIGIN      
        1 ataaatggcg ctgtcctcat gtctgtttaa aaagcgttaa ctgtccttac tttaacagac
       61 ctgaagatga ttacggaaga acatattaca ccaaatttga tgatgatatc cgccttttta
      121 cacctacagt tagagggtca aaaaaatggg aaaacacaat gaataaaaga acttcttctg
      181 aacgttgtaa tgaccgtatt aaaaacgatt atcggcttca aagtgataaa gttcgatcta
      241 aatctcgttg gttattacgg attatcatga gagatgctgc tatgcatgta aatgcctggg
      301 ttaatactgc tcatctttct tctgaaaaat ggctttcttc ctggtttagc ctagaacaaa
      361 cagcttaact aaatttcaat ttatcctctg tttaaacaag ggataagtgc gcccattttt
      421 aaggcctatc taatttaaaa cagttctaaa atggagtttc caaggttcat ggtctcaaaa
      481 cttgaaactt tatagtcaaa ttcaactttt tatatctttt tatatattag ccgagcctac
      541 tctaatttgt cactgcaata aaaattatcg tgttattttg caatacttaa ttataaattt
      601 aaatatttaa atctatgaca tttttaataa tacatatttt ctccataccc attggtagaa
      661 aacaagtaac ttgtctgtta tattaataaa tattgtcctt tttaattgag aaaaaatata
      721 atattagtct cattataatg aaataataga agttttgtca cataaaagat atgggcaatt
      781 ataaactaat aatttttata attttatcaa ctgttttgga gtagaaccaa taatattaag
      841 tatatttata atattatgaa atttaaatct agtttaatta ttattagaaa ttttattaaa
      901 tataaatatt tttaccaatt agttatacaa tcaaaataac ttttataaat taagtaaaaa
      961 tttatcttat aatatagcta tatttctatt aataataaat atattaggaa ttgttcattt
     1021 ttcttactct ttgttgaaaa attaattaac ctacattctt tattcttatt ttcttctcgg
     1081 ttaaaatttc tcaagtcttg aacttaccaa caataattag ataatgctta atcgttattc
     1141 tcttctccca ggtaaattcc attaaaaatt taatctttat ttaattatta atatcatcgt
     1201 ttatttctgt taaatattta gtttgttctc tagtttctat attgtcagtg tttaaaagga
     1261 ttatcccccc cattataatt aattatttga ggtaagaact ttctaatata aaatttttat
     1321 ttctcaatcc tttataactc cttctagttt gtttattatt acttgtcaac tgttgacaac
     1381 atattaaaaa aattatcgtt tatcagacca tttctgaaaa aatacgaata tgatctatta
     1441 ctgctttttt agtttcttta acactttctt tttttaagta ttttaatata tctttatgtt
     1501 ttaaacttgc ttcttttagg ttttcttctt ttttagcgtc tattatcatg gctaattgta
     1561 attggtaata aagtgtagta agaatcttat ttgtccattt actattcgct tttttccata
     1621 gataactatg gaatttcata tctcttttgt ttacttcaaa gatttttaag ttatcatcca
     1681 tatcactttc tgtaatttta accatctctt caactagact ttcaaggtaa ttaaaatctt
     1741 cttcattcat tttttcttcc tctatcaaaa tcttaaatac tctttcttct aacattactc
     1801 taatatcaaa aagttcttga atttcatcaa gttcaaactc aactacaaat gtacccttcc
     1861 ttggaatagt ctctactaaa cctttattct ctaattcttt gatcgcctct cgaacaggtg
     1921 cccggctaat atcaagttca tctgctactt tagattcaat tattctatct ccgcccaaat
     1981 aattttctaa aaagatctgt tcttttaaat aatcaacaac accacttttt aaacttttag
     2041 gagtgaactt ctctctttta ctcattttta tccccccgga taactgatat tatataatat
     2101 aatttattac tctcctacta tatactttct tgagttttgt tttgactcct tgaattttta
     2161 taatatcagc tttaaactaa cattgagctt tgatttaata aacaatttat gggcaaagtc
     2221 atataaatct tcacagctgt attcagatat tctttttaat gagtttattt cagatcaatt
     2281 ttcattttgc tcgaatattg ataaaaagtg aaaggtcata ttttgtttaa atattctttt
     2341 atcatatggc ctcaaacttg cagccggttt gacccaaaca ggtggatagt tgaattcaaa
     2401 agcataacca agtatggaaa tatcaatttt tcttcaacta tttcatagaa tacacatagt
     2461 ttaacgaaat caagtatttc atgtaatctt tccaaaaaat atttaggtat ttctttcatt
     2521 cttcatactg attcttaaaa ttaaattatt ctaaatattg gagtattata caaccgaata
     2581 attctaagat aacaagctta ttttaatata gcatcttcca attactcaat tctatattct
     2641 gattccctaa ataacaattt tactcctcca tatttgaagt ttaaacccag ccgcgcatct
     2701 taacggcttc agctactttt ctaagtgcta ctatccaggc acctaatctg gttgtagtat
     2761 caaattcttc agcagtatct cttgcatttt tataggcagt taagatctgt ttgtttaatc
     2821 ttttttcaac tttttcaata tcccagtaat catctgtaat tccctgaatt ctctcatagt
     2881 gacagacaat tgctccaccg gcgttggcaa caacatcagg aataactttt atgccattat
     2941 cctttaatat agcttctgct tccggggtaa taggaccatt ggctgcctcg attatcattt
     3001 tagtattgat attatctgca ttttggccgt taattacatt ttggacagct gccggaatta
     3061 aaagtccaca ttctaattcc agtagttctt cattactgat actttcagcc tcactatatc
     3121 cttctaaccc attattttct tcgcagtatt tttgcatggc gggaatatct agaccgtctt
     3181 tattatagaa gccgccgcta atatcactta ctgctgtaat ttttgctcca atatcataaa
     3241 gatagcgggc aattgtagct cccacctgtc caaaaccttg aatgacaacc tcttctttgg
     3301 caagatcaat gttattatcc ttactggctt cttttgttac atagaagatt cctaatccgg
     3361 tcgcttctgc actgccgact gtcccaccca ttatattggg cttgtcatta atggcaggtg
     3421 gattatgttc gcctgtaatc tgttcatatt catctaacat ccaggactgt gtcctataat
     3481 ctgtaccaat gtccgcacct ggtatatcga tcttaggtcc tttgggttta aggtttctaa
     3541 taaaacctct ggttagtctt tctagttccc agtcacttaa cttggaagga tcaactttaa
     3601 taccaccttt gccacctccg gctggtacac cagaaacggc atgtttaatg gtcataaata
     3661 aggctaaggc cttaacttca tcgatgtgta aattgggaac aaatctagtg ccatctctag
     3721 ttggacccag ggcatcatta tagtgaactc ggtaggcatc aaacatttta aagctgccgt
     3781 catccatttt aagcggaata gaaaaatcaa ttatcctttt tggccttgac attagctcca
     3841 taacattagg atctattcca gccaattcac ctgctttgtt taaagaatct aatgcggaat
     3901 taaaaacatt atccatatat ttctacctcc atatttcgta aattttatac cttattatta
     3961 aaaatattat taaaggttac attctatatt aattactcaa cttcttaata tgttggaaat
     4021 aaaaccagct gttattacaa cagctggtat catcttttta gacctgtggt actgagagat
     4081 cttctttata atcattcatt acgatatcat gttcacttct atctatccat tccatgataa
     4141 tttgtgctat tttgactctc atcttggcta agttatctaa ataattggga ctttgggaat
     4201 caatccaact attgtataag tctgcttccg atggcccggg ctctattttg aaatataccg
     4261 gtgatgctat tctggctata tcagctgctt catcatatct ttggaagccg cccattgatt
     4321 caacaaggct catatataca tctagaggta ctttaactcc accctgtcta atggaagaaa
     4381 gcatcggtaa agtcatatca gccagaggat taaaactatc tgctcctaat ctttccaaca
     4441 tttttccagc aactgcagta ccgtgacctg caaaaacaga aactttaaac ttaacatcct
     4501 gaggtataat acccttatcc ctcatatcat ttaataaggt taaagcagat tcatctgtaa
     4561 tcaaaaatcc acgaatacct gcttcaatac acctgtctat gttttttaga tatctataaa
     4621 gcatatcatg acctctcaac ctcatcccgc ttacaatacc ttcttcacca gatgtatact
     4681 ggcggccggt gtcccattct ctgctagcta caggattaac aagcagttca aggtcattat
     4741 ctgcccctac ttgagcaaag tgctttaatt cctgatcatc taacattgtt gcccctctta
     4801 caattgaaat tacacggtga attgtaatat ttcttttatc tgcttctttt accatctctt
     4861 ctaaattaga agcggactca atacctgata tttctattct ggcatgagca ccacctggga
     4921 aagttaattc tgaatccggc atatcatacc tttcccttaa tgaatattca gtgtcgtttt
     4981 taatgtaagc agccatttct ttaaatgcca tttaaacaac ctcctattaa tattttatgt
     5041 ttatttattt tattcaaatt cagaaaataa ttcttcaaaa tcttcttcac tatcttttat
     5101 atgatagaca tgttcatctt cagggaactt ctcatttttc acatcatcaa tatattcttt
     5161 aaaagcttcg atttcaatct ctgccacatt ggcatactgt tttacaaact taggtgtaaa
     5221 ggcctggaac atacccaaca tatctccaca gataagcagc tgtccatcac agggtaagcc
     5281 ggctccaatc gaataaactg gaatatctag cttatccgta ataaattcgg taacttcagg
     5341 tggaactgct tctaatagta aggcctgtgc tccggcttcc tgaactgcta ctgcatcttt
     5401 gattaattct ctggcactat ccacagtcaa gccctgagcc ttaaaaccac ctaactgtcc
     5461 tgaactttgg ggagttaatc caatatgtcc aaaaactaca atacctgcat cactgatcgc
     5521 ttcgatctga ctggcaactc tgcggccacc ttctaattta attgcatcca tatcagcctc
     5581 ttttaaaaat ctagctgcat tttctaccgc tttttcatct gatacctgat aagaaccaaa
     5641 aggcatatct cctatacaat aagtgttggg agcacctctt ctaacatcct gactgtgggc
     5701 aatacagtct tccattgtga cgggaactgt gcccttatat cccttaacaa ccattcctaa
     5761 ggaatctcct accagaatca tatccatgcc cgctttttca gcaaattggg cagttggaaa
     5821 atcatatgct gttatccaag caacattttc tcccttttgt ttcatttcat taaaatctaa
     5881 aatagttttc tttttagcca ctacaatacc tccttatatt ttgatggtta actttgaaaa
     5941 tatcactcat aaaatttgag tatttaaatt tatcaatacc taaaaatagt atgtattaat
     6001 ttaatttgtc gattgtcgac aatatacact aactaattta ttcaatgcta gatttaaaag
     6061 tcctttttaa attttaaaaa aaattataat tttcagataa ttaattgagt aaggaaattt
     6121 taagaactag gcctgaaggc cggcaattca tttatgagaa ttttttctca tttgaagaac
     6181 atgctacttc ctttttttct tttacactca aaaccaccat agctgaccca cctaaaacta
     6241 aaattgctcc gattatctga taaagattta aaatgtcatt gaaaaatatg gtcgataaga
     6301 cgatggttac aatcggttct atcatactta aaaccaaaat ggaagtggga ctggtcatct
     6361 ttatcccctg gaaaaaggcc aaaaacccta acatggagac aaccgcgatc cctataactg
     6421 gaagccaggt attagaagtg aatgcaaatt gaacattatt actaataaga ccgacagtta
     6481 aaaaagaaat agcggcaaat agacagacca tagctatcgt agtaagtaag ggcacatctt
     6541 ttagaatgtg gtctccaaat agagtataga cagagtaaaa taccgctgct ccaaaggcaa
     6601 ataagagacc aacaaggaca atatcaccac ttgttttacc cagaacaaag gtcaaaccta
     6661 taaaggaaac gattattgcc cccataacct ttttagttaa cttcatgttg agaaaaaaag
     6721 atgaaatacc ggctgtaata aagggataag tataaaaaat caatacccgc tagcgaggcc
     6781 agtatgtacc ttatagcaga gaaataacaa aaactaaaga cagaatataa aataccgccc
     6841 atgacaaata tatttaatat ctgtttttta tttaaaataa tctttctgtt ggtcaaaaag
     6901 atatataaaa aaagtaagat cgaagctaaa gtaaacctta aaaaaagcag ggtaaacaca
     6961 tttatcccac tgtcataggc aaataaagct aaaattggta caataccaaa accaactgca
     7021 gatattactc cataagtaga acctaatata ctttcatcct aataattcac ctcctcttta
     7081 aatgataaaa tatatttact cttaatacac ctctttttat atgtttgttt aaaattcttt
     7141 tttagttatc aaattttatt tctctacctt tacgctcaga ttcatatact gcatccaata
     7201 ttttggttac catataggcc tcttccggtt ttactagcgg acttttatcc cttattatgg
     7261 cattgatcca gctagccgct tctaagtatc ctagacttac cttttcactg gcagccggtc
     7321 ccattttttt aatagaactt tctttaattt ctttatattg accttcctct atcatattat
     7381 atataagttc atttttatcg tcacttaaat tccaccttag ttctgctccc ctcttcgtcc
     7441 cataaaaggt ggcggacgct tcttttggat ctctcgtatt taatatccag gcagattcta
     7501 gaaatacagc tgccccattt ttcattttta tgaaaccaaa ggcagagtct tctaccccat
     7561 atttattagg attccagggg ccaaataaat tacccttagt ttgatcactc atcttattaa
     7621 aaaccgaacc tgaaacagta accggttcat aattgtccat tatccacaga gttaagtcta
     7681 aagcatgagt accgat
//
LOCUS       DATGCJ010000218        16860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_255179, whole genome shotgun sequence.
ACCESSION   DATGCJ010000218 DATGCJ010000000
VERSION     DATGCJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 16860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16860
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255179"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VKN64_09800"
     CDS             complement(<1..1716)
                     /locus_tag="VKN64_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMA60575.1"
                     /translation="MKKWNEYSLRTQVIIVFGLIQILLFGLIFYFITLNYRDFYLDQV
                     EENLKNNIKLIKNDETIKNQMGNIDQLDELIKEIGTSIETRITIIEVSGKVLADSRYN
                     PYIMEDHSNRPEIKAALEGSDYGVSERKSSTLNQDMYYVASVDRLNGKRVVIRLAKSL
                     ENINQIIRGDVYKYLLFLIVLLILSNTLVWYYSKSIIKPLKRIKNMAKEIAAGHRNKI
                     DDIKYSNSELGSLVHEYNRLGEELDSKIDKLRDEKNKLTAILNSMNEGVIATDDNKKI
                     LMINPKACEILNVNCGDVEGKNFISEIRDHRFDEYLNKVLEKKENYSTEMTFKNPDKR
                     YLECNFQTITDHKDEIIGAVMVLIDVTRLRKLEEMRKDFVANVSHELKTPLTSIKGYA
                     DTIVENEIKDFDTIKRFVGIISKEATRLNLLINDLLDLSKLEADYFELKPEELDNILD
                     KPLRILENEADKKDIEIIKQFEEDLSLVKMNKSQIENLLINLIDNAIKYNKEGGKIFI
                     RAYQENGKVYIEVEDNGFGIPKEDQDRIFERFYRVDKARSKEVGGTGIGLSIVKHIVK
                     GHNSEI"
     gene            complement(1700..2389)
                     /locus_tag="VKN64_09805"
     CDS             complement(1700..2389)
                     /locus_tag="VKN64_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA60576.1"
                     /translation="MSEKILIVDDEKNIRDLLKFNLENEGYETLEAKDGKEALEKVKE
                     DIDLVILDLMLPEVDGLNVCKQIRNDDQIGNLPIIMLTAKSEDIDRIIGLELGADDYI
                     TKPFNTRELIARVKALLRRISMSVGYEKEEKKIIKLNDLRINIKNHTVKEKDQEISMT
                     PKEFDLLVFLIRNDDQVFSRDKLLSEIWGYEFSGDTRTVDVHVRRLRKKIGEDLIKTV
                     RGVGYKFEKVE"
     gene            complement(2443..4101)
                     /locus_tag="VKN64_09810"
     CDS             complement(2443..4101)
                     /locus_tag="VKN64_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HMA60577.1"
                     /translation="MSFEMLVGLTGGLGLFIYGMKQMSEGLQKTAGKRLRHILAFLTK
                     NTFSGILVGTGVTSIIQSSSATTVMVVGFVNAGLMTLYQSIGVIMGANIGTTVTAQII
                     SFDLGDYAFHAITIGAIAYLFAQRKRYQYIGQVILGFGILFLGLNIMSETMHPLRESQ
                     YIVDLMKSFGTYPILGVLAGMAITVTVQSSSATFGILVGLVSVGIIDFSSGVPILLGS
                     NIGTTVTAILSAIGASKTAKKSAAAHFIFNILGTSIMIAFIYLIPNFTGRLQSLLRNF
                     SLMTSNSVSAERMLANTHTLFNVTNTLVWMPFVGFMVSLVNKIIPGEEVSIERGLVHL
                     DERMLSTPQAALSQVNMEVTRMHSLVQEMVIESKQAVLEKDTDIIKKIRHKEEIINEI
                     EEELLVFLTDIPQSSLSNEDIKTLDSYFAIVDDIESIGDDGNSIADLAEYIIDNNLEF
                     SEEAIKSIETAYDIVIDYLEDSREIVRTGDRDIAEKLFTGEEKMDKILLQYRNEHLQR
                     LNKGVCIPSAGIAYLEVLEDLEHISDQFADIAYSIIERKKKKIG"
     gene            complement(4166..4426)
                     /locus_tag="VKN64_09815"
     CDS             complement(4166..4426)
                     /locus_tag="VKN64_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HMA60578.1"
                     /translation="MPKNTLSEIRRNISQYKGKEVIIKADRGRRRSINKEGILDETYD
                     NIFIVKIQESEQERHLSFSYADLLTDSVELKLKEDHQRIGVI"
     gene            complement(4544..5020)
                     /locus_tag="VKN64_09820"
     CDS             complement(4544..5020)
                     /locus_tag="VKN64_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HMA60579.1"
                     /translation="MIFISPTEGKLNIKETYKSILSFIKKQPNNHYKLIIGTDSHPGI
                     EESVFVTAIVIYRVGHGGRYFYHKKKVEMRSGFKKRIFHEVSRSLNVASKITDYISID
                     SATSSLDIEIHIDVGQNGPSSEIIKEVVGMVVGSGYDALIKPDSYAASNVADRYTK"
     gene            complement(5026..5916)
                     /gene="rsmA"
                     /locus_tag="VKN64_09825"
     CDS             complement(5026..5916)
                     /gene="rsmA"
                     /locus_tag="VKN64_09825"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMA60580.1"
                     /translation="MDKEIATFHNTKDILNKYNIRLNKNLGQHFLIDSKVLKQIIKSS
                     NLQGDEKVIEIGSGIGTLTQYLLEELDEGQIFAIEKDNRFINILKDLFQDNDNLKIIN
                     EDILQINWERFIKSNQLAGEDLKIIANLPYYITTPIIESLLFSPLQINEMVFMMQREV
                     AKRLAASPGNKDYGSLSVFIQFHYQVDIQFEVPPEAFIPKPEVYSSVIKLIPHAEIPY
                     HVKDKDFFFQVVKAIFNLRRKNIKNGLTLSPHVKLDKEIILKGLENTGIDKRIRGENL
                     SIEKMVQLSNNLWDLKKEKE"
     gene            complement(5998..6609)
                     /locus_tag="VKN64_09830"
     CDS             complement(5998..6609)
                     /locus_tag="VKN64_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMA60581.1"
                     /translation="MGKIKVLIFDKHKLYKDALSHILKDKEKIKSVFSISKYEHVLPV
                     LDKFYPDMVLINSLKVEHDKLEQLVKGIKNKDNEISIFLLKNEDTFLHRNTDRFVDLS
                     IPITKDHKYLYKMIQKYVSNNESNTVEESVTSYKFHNLTDREMDVISLISRGMSNKEI
                     SEELIISERTVKNHVSNILKKLSVNDRTQALIKCLKMGIVKLE"
     gene            complement(6726..7355)
                     /locus_tag="VKN64_09835"
     CDS             complement(6726..7355)
                     /locus_tag="VKN64_09835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMA60582.1"
                     /translation="MIKAGQIFDISITIKEGMVSFPGDTVPEIERIKEIEKVGYNLSK
                     MQVSVHVGTHADAPTHFVEDGKYIDEIKPERFMGETQVIEIENNNEITKAELEDKEIK
                     SDKLLFKTINSEYLKEDTFNENFVYMTYQAAEYLVKKGVKLIGIDYLTIESLDNKDFA
                     VHKLLLGNDVIILEAVNLANIKPGLYNLLAFPLKLQNCEASPIRALLTK"
     gene            complement(7368..8324)
                     /locus_tag="VKN64_09840"
     CDS             complement(7368..8324)
                     /locus_tag="VKN64_09840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HMA60583.1"
                     /translation="MAKVFVTRKIPDAGLDLLKEKYEVEVFSNTKNPTKEQLIEKAKE
                     ADALIPLLGDEIDEEVINKCSNLKIIANYAVGYDNIDLKAAEENDIYVSNTPDALTET
                     TADMTWTLLMAAARRVIESDKYVRDLKFESWGPELLLGQKVYGKTIGIIGFGRIGQAV
                     AKRAAGFDMEILYNKRTPLSDKEEKELKASYADLPDLLKRSDFVTINAALNESTYHLI
                     DKEELKMMKSCAVLVNTGRGPIINEKVLAEALADKEIFAAGLDVYEEEPEVHPKLLEL
                     DNVVLTPHTGSATVDARNKMAEMAAANVIAVLSGSKPVNQVV"
     gene            complement(8428..9564)
                     /gene="buk"
                     /locus_tag="VKN64_09845"
     CDS             complement(8428..9564)
                     /gene="buk"
                     /locus_tag="VKN64_09845"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HMA60584.1"
                     /translation="MKYKYIPRKKERFLMYNILVINPGSTSTKLSIFEDEKEKWKQEL
                     QHSSEEIKKYAKIIDQLDWREKLVREEIKSSGFNFNDLDAIVARGGVALDPLLGGTYL
                     IGDDMVHDLEIAKNSSHASNLAGIIAYNLGEEFGIPAYTVDPISVDEFEDVARLSGLP
                     EIPRKCQSHALNLKSIGRKTAEYIDKKFDECNLVGAHLGGGISIAPLKKGRIVDVNNA
                     NQGGPYSPERVGTLPSLDLAEYIFENQPERDKFVKKLLGNGGLTAYLDTSDGLEIEKR
                     IEEGDKKAELAYEGMIYQIAKEIGAMATVLNGDVDAIFITGGLAKSKYITNRVIKRVE
                     YIAEVFVYPGAAEMENLALGALRVLKEEEDILRYEEGKMDTTIF"
     gene            complement(9588..10394)
                     /locus_tag="VKN64_09850"
     CDS             complement(9588..10394)
                     /locus_tag="VKN64_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMA60585.1"
                     /translation="MDLQKMYFDYNQIKEEVTSSWSRCLKYNRKREIDTTQLREFPTQ
                     KKYIDNSIKKYFKIKVGEINNKLEEKPCFFLVNKNFVIEAISADFNKKIKLEQVGIEE
                     GFSFAEEKSGTNAVFLAGKLGKPVHILPGHHFCKPLNSWHSIAYPIIIKEDIYGFLNI
                     VSQNKIKNEIICLTELLVNNLELKISNKNRERQDISYHKNLSDKQFYILKKIAHGLTE
                     RALASDLNLSKSTIKYHKKKLFNFFNADSNIEMVVKAFQKGFLTFNEVSF"
     gene            complement(10395..11555)
                     /locus_tag="VKN64_09855"
     CDS             complement(10395..11555)
                     /locus_tag="VKN64_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMA60586.1"
                     /translation="MNYKYLDIKNLSQKRYKNNIKSKLFLKILLLSIILIIIGLTIWL
                     IYGEVDVIIKANGVIQNEENISYIYSIKGGYLDYIIPPKNQIVRKGELLFSLSNQELL
                     KRKRYLKNKLKLLELKISDLQVIKRFIREKDISIKLNTQLFQNELTVIKGKLKHYKYN
                     IKLKKDKYKKLKKFEGLSITKNRLINEKKQIENLEYELKEYKQNKLVELEKRISSLED
                     AIITKKIELDKINSDIKKYKICAPIEGKIEYLNKYNTGDYIPGGVKVMKIIPKTQNKY
                     VVDLIIENKDILKLNKGDEVVYKIASFPYKEHGTARGRILKINSDTTKLNDNNYIYKV
                     KASIKNFLEKGSNKKYVTYKNGMLTKASIVVRKRKIIYYILEKLDFLNRGVF"
     gene            complement(11581..13767)
                     /locus_tag="VKN64_09860"
     CDS             complement(11581..13767)
                     /locus_tag="VKN64_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HMA60587.1"
                     /translation="MQKLTTAVYLIFLKLIKYSKFIKQRDQSDCGSACLATVANLYNL
                     NYPISKIRERAGTNQKGTSAYGLIKAAVDIGFKAQGVKAEMKDLNQNIKLPAIAHIIE
                     NDILHYIVIYRIKDKAMIIFDPASGLRTIKKNEFKEKWTNILILLTPEQKERSKAKDL
                     DKISFLKYHLKDNKGLLIQIFIASLFYTAFGIIGSFYFKYLIDSILVNGLKNTLHIIS
                     MGALVLAVMKVVMDTFRRQLSLYFSQQIDIRLIADYLEHILTLPISFYERRAIGEILS
                     RVQDSGKIREALSGAAITIMIDSLLVIGGGIILYLQSSYLFKIAVIIIPAYITLVLLF
                     AYKHKRVRKEEMEKGANLQSNLVETVKSIKTIKASNNEKSSYLQNEDDLITFIEKAFK
                     ANFLKNMQGSIDNLLAAVGEIIILWSGGYQVINGNLTVGQLITFNALLAYFYKPLQNL
                     IKIQPKIQQASAALDRLLEIMVLDSVGKDDNLIKLESIKGKIKYENIEFIYNMEKRVL
                     KDLSFEIKPGQRIALVGKSGSGKTTIIKLLLKFYSSYQGNIYIDGKNIKDIETKSLRD
                     KIGYVPQEPYIFNKTIRENINLNDEDYSLEEIIKACKKAKIHNYINQLPARYETILNE
                     NGSNLSGGQRQRIAIARIMLKDPDLIIFDEATNHLDYETEKSINKLLKNTIKNKTVII
                     AAHRLSSIKNSDNIFYIDRGQVVEKGTHQYLLRQKGQYHSLWQVQQ"
     gene            complement(13781..13936)
                     /locus_tag="VKN64_09865"
     CDS             complement(13781..13936)
                     /locus_tag="VKN64_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60588.1"
                     /translation="MNNKENKKMSLLTLLSGPVQGHNLVLGTFFSTALGVIVGFVIGQ
                     LIYQLLQ"
     gene            complement(13960..14082)
                     /locus_tag="VKN64_09870"
     CDS             complement(13960..14082)
                     /locus_tag="VKN64_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60589.1"
                     /translation="MDIKGGFVVSSTTATVVYLMAGLGGIALGAYVGYNKAANQ"
     gene            complement(14127..14300)
                     /locus_tag="VKN64_09875"
     CDS             complement(14127..14300)
                     /locus_tag="VKN64_09875"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HMA60590.1"
                     /translation="MDKAKSKLTPLSKDELMQVQGGGPISGPTLILGAFGVASAGIAI
                     GLAIGAACYYSTR"
     gene            complement(14585..14752)
                     /locus_tag="VKN64_09880"
     CDS             complement(14585..14752)
                     /locus_tag="VKN64_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60591.1"
                     /translation="MKYLVKYEKEGIEEVVEAHAPGQATYHFLKDHNIDIEEIDQDDV
                     KIIPYNEEDEK"
     gene            complement(14834..15613)
                     /locus_tag="VKN64_09885"
     CDS             complement(14834..15613)
                     /locus_tag="VKN64_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMA60592.1"
                     /translation="MVELIDSHAHLDFDKFNKDREEVIERAKSNGIVNIINVGSNLTS
                     SHRSLQLSQQYEIIYAVVGVHPHEAKTLDKKALQVLKDLSKADKVVGIGEIGLDFYYD
                     NSPRDEQREAFKKQLQLAKEINLPVVIHSREADEDTIKILKEENMRNHNILLHSYTGG
                     AEMAEEALELGFYFSAGGIVTFNSAQDLKNIMQKIPLSRILLETDSPYLAPSPHRGKR
                     NEPMYVKEVAKYLASLKNTTFEKVAETTTSNTRRFFNIEKN"
     gene            complement(15643..16245)
                     /locus_tag="VKN64_09890"
     CDS             complement(15643..16245)
                     /locus_tag="VKN64_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60593.1"
                     /translation="MFEDLNQKINKLEGIISCKITGDNDIDEVHIIADKKRNPKRIVR
                     DVETIILVNQDKDIDHKKISIAQFEHDDEKHDIDRLEIISIFKENNNPVCHYRLKIND
                     NIFEGTSDDYPDEPLMYKAANGMAKMLEKYIKLPGKLRVENVFKTGLRDDIIVVQITL
                     YNEHGMEERLLGATYVNQDLPLAVGKGVLKALNRKIIGSY"
     gene            complement(16292..>16860)
                     /locus_tag="VKN64_09895"
     CDS             complement(16292..>16860)
                     /locus_tag="VKN64_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMA60594.1"
                     /translation="VIESFLKVIDSKDHYTEGHCERVANYTKQLCDVAKVRHGRKERI
                     VDMAKIHDIGKIYVDDEILKSDEVLSEEEFAEMKKHSYYGYELLKDIDMLNDDLEIIL
                     HHHEFYNGNGYPDGIEEKEIPLGSRILNICDSFDVMTTGRSYKRALSKKETIEEFEEC
                     SGEQFDPELTAKMIELIKSGELDNSFKK"
BASE COUNT         4996 a         3243 c         2176 g         6445 t
ORIGIN      
        1 tatttcacta ttatgacctt taacaatgtg ctttactatt gaaagaccta taccagttcc
       61 gccaacttct tttgaacggg ctttatccac gcggtaaaat ctttcgaata tcctatcctg
      121 atcttctttg ggaatcccaa aaccattatc ttcaacttca atatatactt taccattctc
      181 ctggtaggcc ctaataaata tcttgccgcc ttctttgtta tatttaatag cattgtcgat
      241 taaattgata aggaggtttt caatctgtga tttattcatc ttaactaacg atagatcttc
      301 ctcaaattgt tttataattt caatatcttt tttatcagct tcattttcca aaattcttag
      361 cggtttatct aatatattat ccagctcttc tggtttaagt tcaaaataat ctgcttctaa
      421 tttagataaa tccaataagt catttattaa taaattgagt cgggttgctt ctttactaat
      481 aatgcctaca aatcttttaa tagtatcaaa atctttgatt tcattttcca caattgtatc
      541 tgcatatccc ttaatagagg ttaaaggggt ttttaattca tgggatacat tagcaacaaa
      601 atcttttctc atctcttcta gttttcttag tctagttaca tctattagaa ccattaccgc
      661 cccgattatt tcgtctttat ggtcggtaat agtttgaaaa ttacattcta aatacctttt
      721 atcgggattt ttaaaggtca tttctgtact atagttttcc tttttctcta atactttatt
      781 taaatattcg tcaaatctat ggtcgcgaat ctcacttatg aagttcttac cttctacatc
      841 tccacagtta acattgagaa tttcgcatgc ttttgggttt atcatcaaaa tctttttatt
      901 gtcatcagtg gcaattaccc cctcattcat actattgagg atggctgtta atttattttt
      961 ttcatccctt aatttatcta ttttactatc taactcttcc cctaatctat tatattcatg
     1021 aaccaaactg cccagttcac tatttgaata tttaatatca tcgatcttgt ttctatggcc
     1081 cgcggctatc tctttagcca tattttttat tctttttaac ggtttaataa tagattttga
     1141 ataataccag actaatgtat tacttaaaat taataaaaca attaaaaata aaagatactt
     1201 atatacatct cccctaataa tttgattgat attctctaat gactttgcta atctaatgac
     1261 tacccttttt ccatttaatc tatcgactga agcaacataa tacatatcct gatttagagt
     1321 agagcttttc ctttcagaaa ctccataatc actaccttct aaggctgctt ttatctccgg
     1381 tctattgctg tgatcttcca taatataggg attatatctg gaatccgcta aaaccttgcc
     1441 gctgacttct ataatggtaa tcctcgtttc tatagaagtt cctatctctt tgattagttc
     1501 atctaattga tctatattcc ccatctgatt tttgatagtt tcatcatttt ttattaattt
     1561 tatattgttc tttaaatttt cttctacctg atcaagataa aagtcgcggt aatttaaagt
     1621 tatgaaataa aagataaggc caaataagag tatctgaatt aagccaaaaa ctattatcac
     1681 ttgcgtcctg agactatatt cattccactt tttcaaattt atatcccact cctctcaccg
     1741 tttttattag gtcttcccca attttctttc ttaatcttct gacatgcaca tccacagtcc
     1801 tggtatctcc tgaaaactca taaccccaga tctcacttag aagtttgtct ctggaaaaaa
     1861 cctggtcgtc atttctgata agaaaaacta ataaatcaaa ttctttgggg gtcatggaaa
     1921 tttcctgatc cttttcttta acagtatggt ttttaatatt gatcctcaaa tcatttaatt
     1981 ttattatctt tttttcttct ttttcataac cgacgctcat cgatattctt cttagtaggg
     2041 ctttaaccct tgcgattaat tcgcgggtat taaaaggttt tgttatgtaa tcatcagcac
     2101 ctaattctaa accaattatt ctatcaatat cttcgctttt ggctgtcagc ataataatgg
     2161 gtaaatttcc tatttgatca tcattacgaa tctgtttaca aacatttagt ccatcaactt
     2221 cggggagcat taaatccagt attaccaggt cgatatcttc ctttactttt tctaacgcct
     2281 ctttaccgtc tttcgcttcc agagtctcat aaccttcgtt ttcaagatta aacttcaata
     2341 aatcgcgaat attcttttca tcatctacta ttaatatctt ttctgacata tttgaaacct
     2401 ccttagaaaa ataaaaaggg accccagagg gtcccattaa tcttagccaa tctttttctt
     2461 ttttctttct attatactat aggcaatatc agcaaattga tcactgatat gttctaaatc
     2521 ttctaatact tccagataag ctatacccgc tgatgggata catactcctt tatttaatct
     2581 ttgcagatgt tcatttctgt attgaagtaa tattttatcc attttttctt caccggtaaa
     2641 tagtttttcg gcaatgtctc tatcaccggt tcgtactatt tctctagaat cttctaaata
     2701 atcaattaca atatcataag cagtttcaat agattttatg gcttcttccg aaaattctaa
     2761 gttgttatct ataatatatt cagctaaatc tgcaatacta ttgccatcat caccaatact
     2821 ttcaatatca tctacaatag caaaataact atctaaagtt ttaatatctt cattggaaag
     2881 agaactctgt ggtatatcgg ttaaaaatac cagtaattct tcttcaattt cattgattat
     2941 ctcttcttta tgtctgatct ttttgattat atccgtatct ttttccaaga ccgcttgttt
     3001 agattctata accatctcct gtaccaggct gtgcatcctg gtaacttcca tgttcacctg
     3061 agataaagca gcctggggtg tagataacat tctttcatca aggtgaacaa gtcctctttc
     3121 aattgagacc tcttcccctg gaataatttt atttactaaa ctaaccataa accctacaaa
     3181 aggcatccaa actaaggtat tagttacatt gaataaggta tgggtattgg ccaacatacg
     3241 ttcagcgcta acagaattac tagtcatcag actaaagttt cttaacaagc tctgtaatct
     3301 acccgtgaag tttggtatga gataaataaa tgctatcata atactcgtgc ctaaaatatt
     3361 aaaaatgaag tgggcagccg ctgatttttt ggctgtttta cttgccccaa ttgcggaaag
     3421 gatagcagta actgtagtcc ctatattact acctaaaaga atgggtactc ctgaactgaa
     3481 atctataatt ccgactgaga ccaggccaac taaaatacca aatgtagcac tactactttg
     3541 cacggtaaca gttatcgcca taccagccaa gacacccaaa atagggtagg taccaaatga
     3601 tttcattaga tctactatat attgagattc tcttaatggg tgcattgtct cactcataat
     3661 atttagaccc aaaaagagaa taccgaaacc taatataacc tggccgatat actgatatct
     3721 ttttctttga gcaaataggt aagcgatagc accaatagta atagcatgga aagcataatc
     3781 tcctaaatca aaggatataa tctgggccgt aactgtagta ccaatgtttg cgcccataat
     3841 taccccgatt gattggtata aagtcattaa tcctgcatta acaaacccta caaccataac
     3901 tgtagtagca ctactgcttt ggattataga agtgactcct gtaccaacca gtattcctga
     3961 aaacgtgttt tttgtcaaaa aggctaaaat atgtcttaat cttttgccgg ccgttttttg
     4021 tagaccttca ctcatctgtt tcatcccata gataaacaag cccaatccac ctgtaaggcc
     4081 gactaacatt tcaaatgaca agtaagtgca cctccagaat gtttactata ttattttttg
     4141 ttgtaaatat ttagggtata tttttttaga taacccctat tctttgatgg tcttctttta
     4201 attttaattc tactgaatca gttaataaat ctgcatatga aaatgataaa tgtctttctt
     4261 gttcactttc ttgaattttg actatgaaaa tattatcata agtctcatct aaaatacctt
     4321 ctttattaat cgaccttctt ctacctcggt ctgctttgat gatcacttct ttccctttat
     4381 attgggaaat atttctcctg atttcactta atgtattttt tggcataaga gaaccccccc
     4441 agatctcttt ttaatagctg atttatttgt aaatttaaac aaatacagaa ctttgtatta
     4501 gtggattata taaaaaaaca tggtgactgt caagttctaa tccctacttc gtatacctat
     4561 cagctacatt agaagccgca tatgaatcag gtttgattaa agcatcataa ccactaccta
     4621 caaccatccc cacaacttcc ttgataattt cactgctagg gccattttgc cccacatcga
     4681 tatggatttc tatatccaga gatgaagtag cactgtctat tgaaatatag tcagtaattt
     4741 tacttgctac atttaaactc ctagaaacct catggaaaat tcttttcttg aatccacttc
     4801 tcatttctac tttcttctta tgatagaaat atctaccacc gtgcccaact ctatagatta
     4861 caatagctgt cacaaataca gattcttcta tccccggatg agaatcagtc cctattatta
     4921 atttgtaatg attattgggt tgttttttaa taaaggaaag aatactttta tatgtttcct
     4981 taatattcaa ttttccctca gttggactaa taaaaatcat ttttatcact ctttttcttt
     5041 tttgaggtcc cataaattat tgcttaattg gaccattttt tctattgaca aattttcgcc
     5101 tctgattctt ttatcaatac cagtgttttc taaacccttt aagattatct ctttatctaa
     5161 ttttacatgt ggagataggg ttagtccatt ttttatattc tttcttctca aattaaatat
     5221 agctttcaca acttgaaaga aaaagtcttt atcttttaca tgataaggaa tctctgcatg
     5281 ggggataagc ttgataacag atgaatagac ttctggcttt ggtataaaag cttcaggtgg
     5341 aacttcaaat tgaatatcaa cttgataatg aaattgaatg aatacactta aactaccata
     5401 atctttattg cccggggaag ctgctaatct tttagcaacc tctctctgca tcataaaaac
     5461 catttcatta atttggaggg gagaaaatag gagactttct ataattggtg tagttatata
     5521 atagggaaga ttagcaatta tcttgagatc ctctccagca agttggttac ttttaataaa
     5581 acgttcccaa ttaatctgca gtatatcctc attaatgatc tttaaattat cattgtcctg
     5641 aaacaaatct tttaaaatat taataaaccg attgtctttt tcaatcgcaa atatttgtcc
     5701 ctcatccaat tcttctaata gatattgtgt taaagtccct ataccagagc caatttctat
     5761 taccttctca tcaccctgta aatttgacga ttttataatt tgttttaata ctttactatc
     5821 aattaaaaag tgctgaccta aatttttatt taatcttatg ttgtatttat ttagaatatc
     5881 ttttgtgttg tgaaaggttg ctatttcttt atccataaat tactccttta tttatattat
     5941 catttttatt ctatcacaat aaaagttcac ttgtaaaata tagagttgat ttatggttta
     6001 ctctaactta actattccca ttttcaagca tttaattaag gcctgggttc tatcattgac
     6061 cgataacttt tttaatatat tactgacatg gtttttaaca gttctttcac ttatgataag
     6121 ttcctctgaa atctctttat tggacatccc tctagaaata agggaaataa catccatttc
     6181 tctgtctgtt aaattatgaa atttataact ggttacactt tcttctacag tattactttc
     6241 attattactt acatattttt ggatcatctt ataaagatat ttgtggtcct tggtaattgg
     6301 tatagataaa tcaacaaatc tatcagtatt tctatgtaaa aaagtatctt cgttttttaa
     6361 aagaaagata cttatttcat tatctttatt tttgatccct ttgacaagtt gttctaactt
     6421 atcatgctca actttcaggc tattaatcaa aaccatatcc ggataaaatt tatccaatac
     6481 aggtaaaacg tgctcatact tagaaataga gaagacagac tttatcttct ccttatcctt
     6541 taaaatatga gacaaagcat ctttatatag tttgtgttta tcaaaaatca atacttttat
     6601 tttacccata ttttcaccca tttatttctt ttaatattat tatatcattt ttttgaaata
     6661 aaaaaaggct aattccttta agaattaacc tgaatttata gtagatttaa acaacaaaac
     6721 tatatctatt ttgtcaataa tgctctaatt ggcgaggctt cacaattttg taattttaag
     6781 ggaaatgcta agagattata taaacccggc tttatatttg ctaaattaac tgcctctaga
     6841 ataatcacat cgttccctaa taaaagttta tggaccgcaa agtctttatt atctagactc
     6901 tctatagtta aataatctat tccgatcagt ttaacacctt tcttaactaa atattcagct
     6961 gcctgataag tcatatagac aaaattttca ttaaaagtat cttcctttaa atattcacta
     7021 ttaatagttt taaatagtag tttatcactc ttaatctctt tgtcttcaag ctctgctttt
     7081 gttatttcgt tattgttttc tatctcgata acttgagttt cacccataaa tcgttccggt
     7141 ttaatttcat caatatattt gccatcctcc acaaaatggg taggggcatc tgcatgggta
     7201 cccacatgca cacttacctg catcttactc aaattatatc caactttttc aatttcttta
     7261 atacgctcta tctcgggcac agtatcaccc ggaaaactta ccataccttc tttaatagta
     7321 attgaaatat caaatatttg acctgctttt atcatttttg cctcctttta gactacctga
     7381 ttaacaggtt tgctgcctga aagtaccgct attacattgg cagctgccat ctctgccatc
     7441 ttatttctag catcaactgt ggcagaacct gtgtgaggtg ttaaaaccac attatctaat
     7501 tctaatagtt tgggatgaac ctcaggctct tcttcatata catccaaccc ggctgcaaaa
     7561 atttctttgt ctgccagggc ttctgctaaa actttttcat taatgatcgg gccccgcccg
     7621 gtatttacta aaaccgcaca tgacttcatc atttttaatt cttctttatc aattaaatgg
     7681 taggtggatt catttaaggc cgcattgatg gtaacaaagt cggatctttt tagtaaatcc
     7741 ggtaaatcag cataacttgc ctttaattct ttttcttctt tatccgatag aggggtgcgc
     7801 ttattataca atatttccat atcaaagcca gctgctcttt tagctacagc ctgaccgatt
     7861 ctaccaaaac caataatccc aattgtcttg ccgtaaacct tctgtcctaa cagtaattcg
     7921 ggaccccaac tctcaaattt taagtctcta acatatttat ctgactctat gacccttcgg
     7981 gcggctgcca tgagtaaggt ccaggtcata tcagccgtag tttctgtgag ggcatcgggt
     8041 gtattagata catagatatc attttcttca gctgccttaa gatctatatt atcatatccc
     8101 actgcataat ttgctattat ctttaaatta ctacatttat taatgacttc ctcatcaatc
     8161 tcatcaccta ataagggaat aagagcatct gcctcttttg ccttttcaat taactgttct
     8221 tttgttggat tttttgtgtt ggaaaatact tctacttcat atttttcctt taacaagtcc
     8281 aaaccagcat ctggtatctt tcgagtcaca aatacttttg ccatttttac acctcacttt
     8341 tttcattatt attcttgatt tctaaattaa tttctgactg attagagata aatacctgaa
     8401 accagcagtt aaactgctgg ttgatgatta aaatatagtt gtatccattt tcccttcttc
     8461 atatcttagg atatcttctt cttcctttaa gacccttaag gctcctaaag ctaaattttc
     8521 catttcagct gcgccgggat aaacaaaaac ttcagcaata tattcaaccc tttttattac
     8581 tcgattggtt atatattttg atttggctag cccaccggta ataaatattg catccacatc
     8641 tccgtttaaa acggtagcca tagcgccaat ttctttggct atctgataga tcataccttc
     8701 ataggcgagc tcagcttttt tatctccctc ttcgatcctt ttttctattt caagtccatc
     8761 actggtatct aaataagctg tcaatccgcc gttgcccagt aatttcttaa caaacttatc
     8821 tctctcaggt tggttttcaa aaatatattc ggccaaatct aaactgggta aagttcccac
     8881 ccgttctgga gaatagggtc ctccctggtt tgcattgtta acatcaacaa ttctaccttt
     8941 tttaagagga gctatcgaaa ttccccctcc caagtgagcc cccactaaat tacattcatc
     9001 gaacttttta tcaatatatt cagcagtttt tctaccgata gatttaagat ttaaagcatg
     9061 ggactgacat tttctaggaa tttcaggtaa ccccgataaa cgggctacat cttcaaattc
     9121 atctacagat atgggatcaa ctgtataggc aggtattcca aattcttctc ccaggttata
     9181 agcgataata ccagctaagt tagaagcatg ggaagagttt ttagcaattt ctagatcgtg
     9241 caccatatca tctccgatta gataggtacc ccccagtaga ggatccaggg caactccgcc
     9301 cctggctaca attgcatcta aatcattgaa attaaaacca gagcttttaa tctcttctct
     9361 tactaacttt tcccgccaat ctaattggtc tataatctta gcatactttt ttatttcctc
     9421 actgctgtgc tgcagttctt gcttccattt ttctttttcg tcttcgaaga ttgaaagttt
     9481 ggtagaagtt gaaccggggt taataactag aatattatac attaaaaatc tctccttttt
     9541 acgtggtata tatttatatt tcattaacca caaccccaaa tcctctttta aaaacttact
     9601 tcattaaaag ttaaaaaacc tttttggaat gctttaacca ccatttctat atttgagtcc
     9661 gcattaaaga agttaaaaag tttcttttta tggtacttaa ttgtactttt acttaagttt
     9721 aaatcactgg ccaaagctct ttccgtaaga ccatgggcga tcttttttag gatataaaac
     9781 tgcttatcac ttaaattttt atgatatgaa atatcttgcc tctcacgatt tttatttgaa
     9841 atctttaatt caagattatt tactaaaagt tctgttaaac aaattatttc atttttaatc
     9901 ttattttgac taactatatt taaaaatcca tatatatctt ccttaattat tattggataa
     9961 gctatactat gccaggaatt taatggttta cagaaatggt gccccggtaa aatatggacg
    10021 ggttttccca atttgccggc taaaaataca gcattagtac ctgacttttc ttctgcaaat
    10081 gaaaatccct cttctattcc aacttgttct agctttatct ttttattaaa gtcagcagat
    10141 attgcttcga ttacaaagtt cttattgacc aagaaaaaac agggcttttc ttctaactta
    10201 ttatttattt ctcccacctt tattttaaag tattttttta ttgaattatc aatatatttc
    10261 ttttgggtgg ggaactctct taattgtgta gtatcaattt ctctttttct attatattta
    10321 agacaccttg accaggagga ggttacttct tcctttattt gattataatc gaaatacatt
    10381 ttttgtagat ccaatcaaaa cactcccctg tttaaaaaat ctaatttttc taatatatag
    10441 tatattattt ttcttttcct tacaactata gaagctttag taagcattcc atttttatat
    10501 gtaacatact ttttattact acctttttct aaaaaatttt ttatagatgc ttttacctta
    10561 tatatataat tattatcatt taatttcgtc gtgtcagaat tgattttaag tattcttccc
    10621 cgagcggttc catgttcttt atagggaaaa gaagcaattt tatatacgac ctcatctccc
    10681 ttattcaatt tcaatatatc tttgttttca ataattaaat caacgacata tttattttgt
    10741 gttttgggaa ttattttcat taccttaact ccccccggaa tataatctcc ggtattatac
    10801 ttatttaaat actctatttt gccctcaata ggagcacata ttttatactt ctttatatct
    10861 gaatttattt tatctaattc aatcttttta gtaattattg catcttctag ggaagatatg
    10921 cgtttttcta attcaactaa cttgttctgc ttatattctt taagttcata ctctaaattt
    10981 tctatttgct ttttttcatt aattaaccta ttctttgtga tactaagtcc ttcaaatttc
    11041 tttaactttt tatacttatc ctttttcagc ttaatattat atttatagtg ttttaatttt
    11101 cctttaataa ctgttaattc attttgaaat aactgggtat ttaatttaat tgaaatatct
    11161 ttttctctga taaatctttt tatcacctgt agatcagata tcttaagctc aagtaatttg
    11221 agtttatttt taagatatct tttcctcttt aataactctt gattagataa agaaaagagt
    11281 agttcccctt ttcttactat ttgatttttt ggaggaataa tataatctaa ataaccaccc
    11341 tttatactgt atatgtaact aatattttct tcattttgaa ttaccccatt ggctttgatg
    11401 atcacatcta cctcaccata gataagccag atagtcaatc cgataataat taaaataata
    11461 ctcagcagga ggatttttaa aaagagtttg ctctttatat tatttttata tcttttttga
    11521 cttaaatttt tgatatctaa gtatttatag ttcaatagga ataacctcat ttacataata
    11581 ttattgctgg acctgccaca aactgtgata ctgccctttt tgccttaata aatattgatg
    11641 ggtgcctttt tctactacct gcccccggtc aatataaaag atattatcag agtttttgat
    11701 actgctcagg cggtgtgctg ctataatcac cgttttattc tttatagtat ttttaagtaa
    11761 tttattaatc gacttttctg tctcataatc taaatggtta gttgcctcat caaaaattat
    11821 taaatcggga tcctttaaca ttatacgggc aatggcaatc cgctgtcttt gtccccccga
    11881 tagattacta ccgttttcat tcaggattgt ctcatatctg gcaggtaatt gatttatata
    11941 attatggatt ttagcctttt tacaggcttt gattatttct tctaaagaat aatcctcatc
    12001 gtttaaattg atattctctc tgattgtttt attaaaaata tagggctcct ggggcacata
    12061 tcctatttta tctcttaatg attttgtctc aatatcctta atgttttttc catcaatata
    12121 aatattcccc tgataagaag aataaaactt caatagcaat tttattatcg tagtcttacc
    12181 cgagccactt ttccctacta aggcaattct ttgacccggt tttatttcaa aagaaaggtc
    12241 ttttaggacc cgcttttcca tgttatagat aaattcaata ttttcatact tgattttacc
    12301 cttaatagat tccagtttga ttaaattatc atctttacca accgaatcta gaaccatgat
    12361 ttctaataaa cggtctaaag cggccgaggc ttgctggatc tttggttgta tcttgattaa
    12421 attttgtaaa ggtttataaa agtaagccaa gagggcatta aaggttatca gctggcccac
    12481 tgttaaatta ccatttataa cttgataacc tcccgaccac aggataatga tctctcccac
    12541 agcagccagt aagttatcaa tagagccctg catattcttt aaaaaatttg ccttaaatgc
    12601 tttttcaata aatgtgatta agtcatcttc attttgcaag taactacttt tttcattgtt
    12661 tgatgctttg attgttttaa tactttttac cgtttcaact aagtttgact gcaggttagc
    12721 ccctttttcc atctcttctt ttctgaccct tttatgttta tatgcaaaca ataatactaa
    12781 agttatataa gctggtataa taataacagc aattttaaag aggtaactac tctgcagata
    12841 taaaattatt ccccctccaa ttacaagaag ggaatcaatc atgatggtaa ttgctgcccc
    12901 tgataaggct tccctaattt tacctgaatc ctgaacgcgg gaaagtattt cccctattgc
    12961 ccgcctctca taaaaagaga tggggagggt caaaatatgt tctaggtaat cagctattaa
    13021 tcttatatct atctgctgag aaaagtataa agaaagttgt ctgcgaaagg tgtccattac
    13081 aactttcatt actgctaaaa ctaaagctcc cattgatatt atatgcaggg tgttttttaa
    13141 gccgttcact aaaatactat ctattagata tttaaagtaa aaggaaccga taataccaaa
    13201 ggctgtataa aacaaggagg ctataaatat ttgaattaac agacctttat tatcttttaa
    13261 gtgatacttc aaaaaagaga tcttatctaa atcttttgct ttacttctct ctttttgctc
    13321 gggggttaag agtattaaaa tattggtcca cttttcttta aactcgttct ttttaatagt
    13381 tctcaaacca ctggcgggat caaatatgat catagcttta tcttttattc tatagattac
    13441 tatatagtgt agtatatcgt tttctataat atgagcaata gccgggagtt tgatgttttg
    13501 atttaagtct ttcatctctg ctttaacacc ttgtgcctta aaacctatat ctactgctgc
    13561 tttaattagt ccataagctg atgttccttt ttgattagtc ccggcccttt ctcttatctt
    13621 actaattgga taatttaaat tatagagatt ggctactgtg gccagacagg ctgatccaca
    13681 gtcactttga tctctctgct ttataaactt actatatttt attagcttta agaatattag
    13741 atagactgct gtagttagtt tctgcatcat ttatcatttc ttactgtaat aattgatata
    13801 ttaactgccc tattacaaat ccaacaataa ccccaagcgc agtggaaaaa aacgtaccca
    13861 gtaccaaatt atgtccctgg accggtccac ttaataatgt taataatgac attttcttat
    13921 tctccttatt attcaattta tagcctcctt gtaatttaac tattgattag ctgccttatt
    13981 atagcctaca tatgctccca gggcgatccc gcctaaaccg gccattaaat atactactgt
    14041 agcagtagtg ctgctgacca caaaaccacc cttgatatcc atcaattctt cctggcttaa
    14101 atttttaata aggtcacctc ttttctttaa cgggttgaat aataacaggc cgcacctata
    14161 gctaaaccta ttgctatacc cgctgaagct actccaaaag ctcccagaat taaagtcgga
    14221 ccagaaattg gtcctccccc ttgtacctgc attaattcat ctttacttaa gggagtaagt
    14281 ttactttttg ctttatccat atgattcccc cttgcatttg tatttcccgg gatatcttat
    14341 ttacaatact atcataatat tccatatttt gccactatat ttatgtacta aatcacttcg
    14401 ttattaatga caattaaata tcaaatattt atcaaatttt aacctattaa taggttcctg
    14461 tagtataata aattcttttg acaaaaaaag ggccatctgc tattacacag atggcccttt
    14521 ttactatcta tttaattata tgttaaacaa gaaatcctgg aacaggccgg gaatttagac
    14581 aatattactt ttcatcttct tcattatagg gtataatttt tacatcatct tggtcgattt
    14641 cttctatatc tatattatga tcttttaaga agtgataggt agcttgacca ggtgcatgag
    14701 cttcaacaac ttcttcaata ccttcttttt cgtatttaac taaatatttc atttaaaaac
    14761 ccctcccatt tattcttgcc tttaattaaa tcatatcata gagaaaataa aagtaaaatt
    14821 gaatacaaaa cattcaattt ttttctatat tgaaaaatct ccttgtatta ctggtagttg
    14881 tttcagcgac tttttcaaaa gttgtatttt ttaaggaagc aagatattta gctacctctt
    14941 ttacatacat tggttcattt ctttttcctc tatgtggtga aggggctaaa taaggagaat
    15001 cagtttccag taaaattcta cttaaaggaa tcttttgcat aatatttttt aaatcttggg
    15061 cactattaaa ggtaactata cccccagcac taaaatagaa gcccaattcc agggcttcct
    15121 ctgccatctc tgctccaccc gtataactgt gcagcagtat attatgattc ctcatatttt
    15181 cttcctttaa aattttaatg gtatcttcat cagcttcccg actgtgaatg accacgggaa
    15241 gattaatttc tttagctaat tgaagttgtt ttttaaatgc ttctctttgt tcatcgcgcg
    15301 gggagttatc ataataaaag tcaagaccta tttccccaat tcccacaact ttatcagctt
    15361 tagaaaggtc ttttaatacc tgtaaagctt ttttatcaag tgttttagct tcatgagggt
    15421 gaactcctac caccgcataa ataatctcat actgctggct taactgtagg gaacggtggc
    15481 tcgaggttaa gtttgatcca acatttataa tatttacaat tccattactt ttagctcttt
    15541 caataacttc ctctctgtct ttattaaatt tatcaaaatc taggtgagcg tgtgagtcaa
    15601 ttagttctac catttttatt atttctcctt ttataatata gattaataac tgccaataat
    15661 ttttctattt aatgctttaa gtacaccttt acctactgct aaaggtaaat cctggttgac
    15721 atatgtggct cctaatagtc tttcttccat tccatgctca ttatataggg tgatttgtac
    15781 tacaattata tcatctctta agccggtttt gaagacattc tcaactctta attttccggg
    15841 taattttatg tacttttcta acatttttgc cataccatta gcagctttgt acattaatgg
    15901 ttcatctgga taatcatcac tggtgccttc gaaaatatta tcattaattt taagtctata
    15961 atgacaaacc ggattattat tttccttaaa gattgatatg atctccaaac gatcaatatc
    16021 atgtttctca tcatcatgtt caaattgtgc aatacttatt tttttatgat caatatcttt
    16081 atcctgatta actaatataa tagtttcaac atcccttacg attctttttg gatttctctt
    16141 tttatcggct attatatgta cttcatcaat atcattgtca ccagtgattt tgcaggagat
    16201 tattccctct aatttattaa ttttttgatt caaatcctcg aacatactct ctacccccat
    16261 ttaacattat gaatttttga atattaaata attatttttt aaaactatta tctaattcac
    16321 cgctcttaat aagttcaatc attttggctg ttaattcagg gtcaaactgt tcacctgaac
    16381 actcctcaaa ttcttctata gtttcttttt tactaagagc tctcttataa ctgcgtcccg
    16441 tagtcataac atcaaatgaa tcacatatat ttaatattct tgaacctaac ggaatctcct
    16501 tttcttcaat tccatcagga taaccattcc cgttataaaa ttcatgatga tggaggatta
    16561 tctctagatc gtcatttaac atatcaatgt cttttaaaag ttcatatcca taataactgt
    16621 gttttttcat ttcagcaaat tcctcttcac tcaatacttc atcacttttt aatatttcat
    16681 catcaacata aatcttacca atatcatgga ttttagccat gtctacgatt cgttcttttc
    16741 ttccatggcg aactttagca acatcacaaa gttgctttgt atagttcgct actctttcac
    16801 aatgcccttc tgtataatga tccttggagt ctattacctt caagaaactt tcaattactt
//
LOCUS       DATGCJ010000219         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_166586, whole genome shotgun sequence.
ACCESSION   DATGCJ010000219 DATGCJ010000000
VERSION     DATGCJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166586"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..731
                     /locus_tag="VKN64_09900"
     CDS             195..731
                     /locus_tag="VKN64_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HMA60595.1"
                     /translation="MKNAVIIYFSNTGNTKKVAKSVYEGLKAKEVEVELKEVSDAYDI
                     DLLDYDLICIGSPSYNWMPPDAMQDFLEVKFDNYENAGQVKLKAPKVEGRNTLIFCTY
                     SGPHTGKKEAVPVVKYIGQFFEHLGFNILDEWYILSEMVGSERYNTQGKMGDIRGLPD
                     EEDLKRIKEKTKELVNKI"
     gene            complement(790..1341)
                     /locus_tag="VKN64_09905"
     CDS             complement(790..1341)
                     /locus_tag="VKN64_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMA60596.1"
                     /translation="MEKIKSISRRHKFLGLLTGLIFGFLLQKGGVTDYNVLVGQLLLE
                     DFTVFKVILTAIITGMIGVNILNYLGKVELSLKTGSFGSIITGGLLFGVGFGLLGYCP
                     GTVIGAVGQGSLDALIGGVPGMILGAGFFANIYPKVKNSFLAKQPIKRISIKDYFDIN
                     DMTFIFLFAGFLIIVLLLIEFML"
     gene            complement(1369..1875)
                     /locus_tag="VKN64_09910"
     CDS             complement(1369..1875)
                     /locus_tag="VKN64_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMA60597.1"
                     /translation="MNILSMGRWSPYIIGFLIGVLSWFSFLFSKRPIGVSTAFARLSG
                     IIEKMLYGPEVEQKEYYQEYKPIVDWEVMLIIGLILGSFISSLLSGQFQFEVVPALWL
                     NAFGSTPILRIITALIGGFFVGLGARWAGGCTSGHGISGTMQLAVSSWLAVICFFLGG
                     VITATLIY"
     gene            complement(1974..3338)
                     /locus_tag="VKN64_09915"
     CDS             complement(1974..3338)
                     /locus_tag="VKN64_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA60598.1"
                     /translation="MLFEKIESPGLAHYSYIVGDQNEAVVIDPKRDIDDYLEKSTKQG
                     FQIKYILETHRNEDYIIGSTNLADKTKATIWHADDQLDYQYGNAVQAGQKFEVGRLTI
                     EAINTPGHTPNSFSYLLYDFSNEPLMVFTGDALFAGDTGRVDLMGEENMDELAHKLYD
                     SIYNKILPLGDGVILCPAHGAGSVCGDTIAEREFTTIGLERKVNPNLQYENEAEFVKN
                     IKEVLERPPYFRKMEELNLKGSNPIDDLPAPAALNPKQFKEKAKEDDVLVLDTRNELN
                     YGAAHIPGSISIWQDGLASFAGWFLSYDKKLLIVTPDKYPAHDIKILNRIGYDNIIGY
                     LKGGLLKWHMAGYESSSFGMIKVQKLCRLLDESNEYFLLDVRSEDELAEAGEIQGAEN
                     IHITQLPDNLDKTPNNLPIYVFCGSGLRSTTAASILKRNGFNQVNVVLGGLEGWSSTT
                     CPII"
BASE COUNT         1094 a          636 c          569 g         1070 t
ORIGIN      
        1 ttattacact ccttgcgatt taagtattta atttaagaac aaaataatat ataatggtct
       61 ataaattaga gtacttactc tacatatttt ctataaatat ttttatttac ctctttaatt
      121 attggattaa tgttttgatt taaacgattt gtattaatat aatattttaa ttaaggaaca
      181 gggaggctga atttatgaaa aatgcagtaa taatttactt ttcaaatacc ggtaatacta
      241 aaaaagttgc caagtcggta tatgaaggat taaaagctaa ggaagttgag gtcgagttaa
      301 aagaagtttc agatgcttat gatattgact tattagatta tgacttaatt tgtattggca
      361 gtccttctta caactggatg ccgccggatg caatgcagga tttcttagaa gtcaaattcg
      421 ataattatga aaatgccggt caggttaaat taaaagcacc aaaagtagaa ggtcgaaata
      481 cacttatctt ctgtacatac tcaggtcctc ataccggaaa aaaagaagca gtaccggtcg
      541 ttaaatacat cggacagttt tttgaacacc tcggttttaa tattttagat gaatggtata
      601 ttttaagcga aatggtggga tctgaaaggt ataatacaca gggtaagatg ggcgatatta
      661 gaggattacc ggatgaagag gatctcaaaa ggataaaaga aaaaactaaa gagctggtga
      721 ataaaatata atgaaaatat agtgaaataa cctggccttc tctatttcaa gaagtccagg
      781 ttattttatt tacaacataa attcaattaa tagtagtact attatcaaaa aaccagcaaa
      841 taaaaagata aaggtcatat cattaatatc aaaataatct tttatactga tcctttttat
      901 aggctgtttt gctaagaaac tattttttac cttgggatag atgttagcaa aaaagccggc
      961 ccccaggatc ataccgggta ctcctccaat taaggcatca agtgaaccct gccccactgc
     1021 tccaatcaca gtcccaggac agtaacctaa gagtccaaaa ccaactccaa ataataatcc
     1081 gcccgtaatt atggaaccaa atgaaccggt ttttaaactt agttctactt ttcccaggta
     1141 attcaagata ttaactccaa tcatgccagt aataatggct gttaatatta ccttaaatac
     1201 ggtaaagtct tctaataata actgtcctac caaaacatta tagtctgtta ctcctccttt
     1261 ttggagtaaa aaaccaaaga tcaaaccagt taataatcct aaaaatttat gtctacggct
     1321 tatggattta attttctcca cctccgatat ttttatattt tagactcttt aatagataag
     1381 tgtggcagtt attactcctc ccaggaagaa acagataact gcaagccagc tgctcactgc
     1441 taactgcatc gtaccactga tgccgtgacc gcttgtacag ccgccagccc agcgggcgcc
     1501 cagtcctaca aaaaatccac caattaaagc cgtgataatt cttagaatag gggtactgcc
     1561 aaatgcattt aaccagagcg caggaactac ttcaaattga aattgtcctg aaagtaggga
     1621 agaaataaat gaacctaaga ttagcccaat tataagcatc acttcccagt ctacaatcgg
     1681 tttgtattcc tggtaatact ctttttgttc tacttctgga ccatataaca ttttttctat
     1741 tataccgctt aaacgggcga aagcggtcga gaccccaatc ggtcttttag aaaacaaaaa
     1801 ggaaaaccag cttaagaccc cgattaaaaa cccgattata tagggagacc agcggcccat
     1861 acttaaaatg ttcataaaaa ttctcctttc tttttaatta actatatact tttatttact
     1921 aaaaattgaa aaaggaggag tatggtattc tatactcccc taagatatat tatttagatg
     1981 atcggacagg ttgtagaaga ccaaccttct aaaccaccta aaacaacatt aacttgatta
     2041 aatccgtttc ttttaaggat actggcggca gtagttgatc ttaatccact cccgcagaaa
     2101 acatagatag gtaagttatt aggtgtctta tctaaattgt cgggtagttg agtaatgtga
     2161 atattttctg ccccttgaat ttcgccagct tcggcaagtt cgtcttcgct tctaacatca
     2221 agtagaaagt attcattaga ttcatctaaa agtctacata gtttttgcac tttgatcatg
     2281 ccaaaagaac tactttcata tccggccata tgccacttga gcagaccgcc ttttaggtaa
     2341 ccgattatat tatcatagcc tatccggttt aatatcttaa tatcatgagc aggatatttg
     2401 tctggagtta caataagtaa tttcttatca taagaaagaa accagccggc aaagctggcc
     2461 aggccatcct gccaaataga aattgaacca gggatatggg cggctccata gtttaattca
     2521 tttctagtat ctaataccaa gacatcatcc tcttttgctt tttctttaaa ttgttttgga
     2581 ttgagagcgg ctggagccgg taaatcatct attggattag atcccttcag gtttaactct
     2641 tccattttcc gaaagtaggg tggtctttcc aatacctctt ttatgttttt aacaaattct
     2701 gcttcatttt catattgtaa atttgggtta actttccttt ccagacctat agtagtaaat
     2761 tctctttctg caatcgtatc tccacagacc gaaccagccc cgtgggcagg acataagatt
     2821 acaccatctc ccaaggggag tatcttatta tatatagaat catacaattt atgggcaagt
     2881 tcatccatat tctcttctcc cattaggtct acgcgaccgg tatcgcctgc aaaaagggca
     2941 tcgcccgtga agaccattaa tggttcattt gagaagtcat ataaaaggta actgaaagaa
     3001 ttgggagtat gtccgggcgt atttatcgct tcaatagtta accggcctac ttcaaacttt
     3061 tggccagcct ggactgcatt tccatattga taatcaagct gatcatcagc gtgccagatg
     3121 gtggcctttg ttttatcagc taaatttgtt gaaccgataa tataatcttc attgcggtga
     3181 gtttctaaga tgtatttaat ttggaaaccc tgttttgtag atttttccaa ataatcatct
     3241 atatctcttt tcggatcaat tacaactgct tcattttggt ctcctacgat atatgaataa
     3301 tgagctaaac caggtgattc aattttttca aatagcaaat aaatcagctc ctttgttaat
     3361 tatctgtaa
//
LOCUS       DATGCJ010000220         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_390839, whole genome shotgun sequence.
ACCESSION   DATGCJ010000220 DATGCJ010000000
VERSION     DATGCJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390839"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            829..2115
                     /gene="ltrA"
                     /locus_tag="VKN64_09920"
     CDS             829..2115
                     /gene="ltrA"
                     /locus_tag="VKN64_09920"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HMA60599.1"
                     /translation="MRKWYSLIDKVYDLTKLEESFYQVKSNRGSRTKGVDNVSVEDFE
                     EHLADNLYQIQQELQTGVYTPQAVKRVYIDKPDGSKRPLGIPTIRDRVVQQALLNILQ
                     PIFEPDFHPSSYGYRPNRSAHHAVAKAERFTRQYGLRYVVDMDLSKCFDSLDQDLILQ
                     SVNKKVSDGKVLQLIDKFLKSGVLDNSEYIPTDKGCPQGGIISPLLMNIYLDNFDQYM
                     KSEGIRIVRYADDILIFADSKSKSGAYKARAEQYLAGKLRLKVNQRKTHLTNLWKGIP
                     YLGFVIRNMGVTISRKSIKKFKDKVRALTPRNKGKSVHYYIRELTMLLRGFSNYFRIA
                     KCKSWFSRLMEWIRRRLRMMIMKAWKSWKPLHKQLRRKGYKGNFEKISMSRWRNSNSP
                     LVNMALTNTWFEENGLYNMKEVTVNTLHQYYNFVLN"
     gene            2417..3658
                     /locus_tag="VKN64_09925"
     CDS             2417..3658
                     /locus_tag="VKN64_09925"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HMA60600.1"
                     /translation="MAINSLLVKLLDNLFEIQGYSLVDIERKTDRVNLFLKNEELPRC
                     PNCGREHSSGRYDSKERTFYLGSLNTTPIYGILRIYRFKCSRCGIVTEDQRISDNKKS
                     YSKNIGEVVTNYTRYLDNVSTSKLLGLTESMVFRIDKDELSRKMEDYIKRVPDKIKSI
                     GIDEVSRKRRHTYATVVTNQYDSKVIWLEKGRSKDSLGSVYNKFSDKLSSLQAASMDF
                     WSPYKSATEKHFPQVSIVHDKFHLSRIFNRHIEQERRDYQRDLPDDERKQIKKHTRWI
                     LLRRRSKNEESYQEHFKKLKERNSHLFEMYLLKEDFLVIFDRKLKKSKAKTAILEWIE
                     KAKESIYENIKSFARKTENRLETLLNWFDHPISNGLSEGINNVIKSLLKRGYGYKDFE
                     YFRMKVLQKCGYLNNSITHTN"
BASE COUNT         1437 a          592 c          788 g         1114 t
ORIGIN      
        1 aaccatatgc tcaacaccgt ggagatttgt aaataaatta tttttattta cattttttgt
       61 ttgccttgca tgcagacata tccaatggat tagttctttt acaaatgaac aaatatcctg
      121 tcgcccaatt ggaagggaag acaatgcgaa gtcaagtgcg tttctggtaa cggaaaggtg
      181 tgaaggaaga cttttagcaa gttagtggaa cataatgaag atgaaccgat tttggcgggt
      241 aattggggtt aggttgcaaa aagtgccaat gcccaaattt ccgaatacaa ttacacgtta
      301 gaacggatgg ctttactaac aggatagttt gcttaacaag ggaagtctgc acttttgtct
      361 aaataggcta agcagaaaca aaccgaaagg taaggatttg ttaagaaggt gcagatgaca
      421 gatgaagccg tagtagtgat aaaatcagaa cctgtaaacc tttagcaata aagttgagga
      481 taaaattctg atgacattgt atataagttt cgcatactta ataacaatga ctataataat
      541 ccaaaagatt attacaggtg cgaagggctg aaattaaatc taagatgtag gaaattttat
      601 taatgcaata agtaaaaaca aagacaatac tggctgaaat gctagtagga attgcaggca
      661 gaagtggcga cgaaccggct tacctgtcaa tccgtgtgag agttgagtgt caatatggta
      721 tccagtatag attgcctaag tgctagagac actggaaaca tattgagaag agacaacctg
      781 cttgtagcat taaatagaat agaatacact gcaaaggaag cgaattaaat gcgcaaatgg
      841 tatagtttaa tagacaaagt ttacgacctt accaagctcg aagagtcttt ctatcaagtt
      901 aagagtaaca gaggctcgcg aaccaaaggc gtagataatg taagtgttga agattttgaa
      961 gaacacttag cagataactt ataccagatt cagcaagagt tgcagactgg ggtatataca
     1021 cctcaggcag taaaacgagt ttatattgat aagccagatg gaagtaagag accacttgga
     1081 atcccgacaa ttagggatag agtagtgcag caagcgcttt taaatatctt acaaccaatt
     1141 tttgagccag attttcatcc ttcgagttat ggctatcgcc ccaatcgaag tgcccaccac
     1201 gcagtggcca aggcagaacg atttaccagg caatatggcc taaggtatgt agtagatatg
     1261 gacctgtcta aatgttttga tagtttagac caggacttga tcctacaatc cgtaaacaag
     1321 aaggttagtg atggaaaagt cttgcaacta atagacaagt ttctgaaatc aggagttctt
     1381 gataattccg aatatattcc aactgataaa ggctgtcccc aaggcggaat aatttcaccc
     1441 ttactgatga acatatatct ggataatttt gaccagtata tgaaaagtga aggaataagg
     1501 atagtccgct atgcagatga tattttgata tttgctgatt ctaagtcaaa atcaggagca
     1561 tacaaagcaa gagcagaaca gtatcttgca ggaaaactta gactcaaagt aaaccagaga
     1621 aagacacatc tgacgaactt gtggaaaggc ataccctacc ttggatttgt tattcgaaat
     1681 atgggagtta cgatttctcg caagagcata aagaaattca aggacaaagt gagagcactt
     1741 actcctagaa ataagggtaa aagtgtccat tactatatcc gggaattgac aatgttactt
     1801 agaggattct ctaactattt cagaatagcc aaatgcaaga gttggtttag tcggcttatg
     1861 gaatggataa gacgcagact aagaatgatg ataatgaaag cctggaaaag ttggaaacca
     1921 ctccataaac aattaaggag aaaaggatat aaagggaact ttgagaaaat atcgatgtca
     1981 aggtggagaa attcgaatag tccacttgta aatatggcct tgactaacac ttggtttgaa
     2041 gaaaatggtt tatacaatat gaaggaagtt actgtaaata ctttgcacca atactataat
     2101 tttgtattaa attagattta atatgagccg tgtacgtgac ccgttcgcac ggttctgtga
     2161 gagggataaa gcgagattaa attaatctcg ctttacccta cttgattgtt tgctcttaga
     2221 tacacaatat taaccgacat aatattgacc gaatcaatca atattactcg gtaatttttt
     2281 tcctatttaa cataggttaa ctagaatttt cagttaaaaa tattggttct tcttcaaaat
     2341 gtatgggttg acataaaaga gtaatggcca taagctattt tgttaataaa aattcatcaa
     2401 agatgaaagg aagcttatgg ccatcaactc actcttagtt aaacttcttg ataatttatt
     2461 tgaaattcaa ggatattcat tggtagatat agagcgtaag acggaccgag ttaatttatt
     2521 tttaaaaaat gaagagctac cccgatgccc taattgtggc agagaacatt catcaggtag
     2581 atatgatagt aaagaaagga ccttctattt aggttctctt aatactactc caatttacgg
     2641 aatattgagg atttatcgtt ttaaatgctc cagatgcggg atcgtaactg aagatcaaag
     2701 gataagtgac aataagaaaa gttattctaa aaatattggt gaagtagtaa ctaattacac
     2761 ccgctacctg gataacgttt ctacatctaa attgctgggc ttaacagaat caatggtttt
     2821 tcgtatagat aaagatgagt tatctcgaaa aatggaagat tacataaaaa gggttcctga
     2881 taagattaaa tcaataggta ttgacgaggt ttcccgaaag cgaaggcata cctatgctac
     2941 agttgtcact aatcaatatg acagtaaggt aatctggtta gagaaaggga gaagcaaaga
     3001 tagtttaggt tctgtatata ataaattttc agacaaacta tcctctctac aagcagcttc
     3061 tatggatttt tggagtcctt ataagtccgc cactgaaaag cactttcctc aagtttctat
     3121 agttcatgat aaatttcatt taagcaggat cttcaataga catattgaac aagagagaag
     3181 agattaccaa agggacttac ctgatgatga gaggaaacaa attaaaaagc atacccgctg
     3241 gatattattg agaagaagat caaaaaatga ggagtcgtat caggagcatt ttaagaaatt
     3301 gaaggaaaga aatagtcatt tgtttgagat gtatttattg aaagaagatt ttcttgtgat
     3361 ctttgataga aagttaaaaa aatccaaagc aaaaacagca atactggagt ggatagaaaa
     3421 agctaaagaa tctatttatg aaaatataaa atcttttgcc agaaaaactg aaaacagatt
     3481 agaaacctta ctcaattggt ttgaccatcc tatttctaat ggcttatcgg aaggtattaa
     3541 taatgtaata aaatcactac ttaaaagagg atatggatat aaggactttg aatactttag
     3601 aatgaaagtt ttacaaaaat gtggatactt aaataattca attacccaca caaattgaag
     3661 gagaaccata tcaatttatt gcgagataat caaaaataga taaagtaaat tttatttata
     3721 aaagggagga gggagaaggc ttcataaacc ctctccctaa atatagggct aatcccgctc
     3781 cgagttaaaa tatgaggatt gtagtggata actcggcaca cccgaagcag ggatatgact
     3841 actttgaaag gaaatcttaa tttgaaataa caatcatttt ttcattcata cactattaaa
     3901 atgatatcaa tagtaaaatg tttcctttat a
//
LOCUS       DATGCJ010000221         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_570833, whole genome shotgun sequence.
ACCESSION   DATGCJ010000221 DATGCJ010000000
VERSION     DATGCJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570833"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..2964
                     /locus_tag="VKN64_09930"
     CDS             499..2964
                     /locus_tag="VKN64_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMA60601.1"
                     /translation="MLKKSLSLIVVICLILILAACSGSNNNLLPEEEYSSLTVTVKDA
                     SDDSNIEGASVILETISSDTTDSEGDAQFEALNVGDYEISVASTGYVAAESEVTIGSS
                     DKTRTIKLAPEQVQQEVTTQSESVGNGLCDMDFNDEDSDQDAKDALSNQTVTVEENDQ
                     DAVEILSNSGTSLGDAGLSQTAQPIGPSFNFSFSTLARQTYHISKVVEDPWVKIKIDL
                     GNLVDNLNNIDSERYSLLVAISTTASSEFTEFIVPENITIDQLLGKICGTIPVTIDGV
                     AIDEIVVTLVYNQDENLDLATDLTDAKNFVGDLKTHGLALRDAGQQQAQKIEDNLKNE
                     VVPYMVSLGYGFHKISDIIEIWGYIVSQAFENEYGVGEFTVNVDNWDTDEAFIISGPS
                     PAENLAELSTWDYTINFTNTGEEDLGTATISITNPIDLYNYEETIEGTYITIDETIDF
                     RNAEFTLDYTEAIGNVNYSLDCSLDSSSYELVEFTDTDTYYDYDELYTEEWNTTVYVP
                     TVSTAQLDGTIEDDTITDEDGVFSDIYGQIFGSTEDIPAEIPVLDSVTINNTLSVDFN
                     NNNDITFNGSFVADAFTYSGNGSFDFIESPLISLENEEYPLLDSINLEMTNFETNVFR
                     ISGGLDVDFTTKTIQESVGYTYDFSVPDEITYQGTYQDISIENGFKLDGTLVIDPYFE
                     DFSIDLTEEYMETEDNYLGGKILLSGSLTNATDANFNLAYFNIYRDGYHHGSTEFRYE
                     FGSGHYIEGEASFGEGQPFKFRAANDQNLSLNMKVYDWSSVTRVGTIKNYYENKLYGE
                     IYTDTDPYVNYSDNTTETLLPSE"
     gene            3045..4115
                     /locus_tag="VKN64_09935"
     CDS             3045..4115
                     /locus_tag="VKN64_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60602.1"
                     /translation="MSKNICIFIIIFIFICPGTLKAGEIGTEINIFLENNIFSVRDGI
                     DYFIDNSIEQEYLQDIIPNNWNLVYNNELCSQISNNIQAGYIREYEGEILAKEDTVLF
                     LIDRINDKYDKYNYSIDYYSFSQSLEGIILEKDIKYSNDICFTINSKLLRGISLSVSE
                     YNGEVEVGEEVLFDGHKQSIQSHLGQETEMYDVDLSSYGFSIGGGVQWFIGSDYWLNF
                     QVEDIINCVFWNDVYTTDINYNDTMLSILSNKKGDEYRAQGDTGKASYREYTTYLTPE
                     YNLSLSNNRLNIGLYYYKKAYPFFSYKILTDKPELKIGLFADYYSVKIKYGCLKALLR
                     TRDLNLMESQGITATLGVNLQF"
     gene            4142..4372
                     /locus_tag="VKN64_09940"
     CDS             4142..4372
                     /locus_tag="VKN64_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60603.1"
                     /translation="MSKNVLGLSRGFIWSRSLPLLKETWLLGFGADTYPIYFPQDDNV
                     GKLITFDKTRKIMDKPHNMYLRLQSILEYLLC"
     gene            4366..4599
                     /locus_tag="VKN64_09945"
     CDS             4366..4599
                     /locus_tag="VKN64_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60604.1"
                     /translation="MLALLVLFVMYFFQSLRLYWNSEFRDIYTITGVGILAAFTGYVV
                     AGLFNDSIISVAPVFWILLGIGISINLKLKTGK"
BASE COUNT         1614 a          669 c          935 g         1532 t
ORIGIN      
        1 tcttcttcaa aaggctcaaa cattaataat cactccaaaa ctctatgata ctttaattat
       61 atcacagata atggttgatt tagggggtaa aaccgaaata attttagtgt tttaaagaaa
      121 ttttagactt ttcgcagtgt aatcattatt aattaattta aaaacctcca tagctactta
      181 aaagtaagag gattagaatt aaaaatatta taaacacata actactagaa ccttcaagac
      241 cataatctcc cataaatttc acctcttttt tttattttgg tatatattat gtgtcaaaaa
      301 gtattttgtg aaaggtgaga aatataattt agtgtaccgc gtaactcctg gtatatgtcg
      361 aaaaaaataa aatacatggt aattacttgc aggattttac tttagtaaag agaagtatta
      421 aaagtataaa aaaataaaga aggaagtgac aaacaccact ttttttaatc atatacatta
      481 ctaaatggag ggtaaattat gttaaaaaag agtttatcat taatagtagt tatctgtttg
      541 attttgatac ttgctgcctg tagtggttct aataataatt tgttgccaga agaagaatat
      601 tcttcactga cagttacagt taaagatgca agtgatgata gcaatataga aggggcttca
      661 gtaatattgg aaacgatcag ttcagatacg actgattctg aaggtgatgc ccaatttgag
      721 gctttgaatg taggagatta tgaaataagt gtggcctcta ccggttatgt tgctgcagag
      781 agtgaagtta caattggtag tagtgataaa acaaggacta ttaagttagc tccagaacag
      841 gttcagcagg aggtaacaac tcaaagcgaa agtgtaggta acggtctatg tgatatggat
      901 tttaatgatg aagattctga tcaggatgct aaagatgctc tgtcaaacca aactgtaaca
      961 gtggaggaaa atgaccagga tgcggttgag atactttcca attctggaac tagtctgggg
     1021 gatgcaggat tatctcaaac agctcaacct ataggtcctt cctttaattt tagtttcagt
     1081 actctggcac gtcaaactta ccatatatca aaggttgtag aagatccctg ggtcaaaata
     1141 aagattgatc tgggcaattt agttgataat ttaaataata tagatagcga acgttatagc
     1201 ctgctggttg ccattagtac aacagcaagc agtgaattca ctgaatttat tgtaccagaa
     1261 aatattacca ttgaccagtt attgggaaaa atatgtggaa caattccggt aacaattgat
     1321 ggtgtagcaa ttgatgaaat tgttgtaacc cttgtatata accaggatga aaatcttgat
     1381 ttagccactg acttaaccga tgcaaaaaac tttgtcggtg atttaaaaac ccatggcctt
     1441 gccttaaggg atgcaggtca acaacaggcc caaaaaatag aagataactt aaaaaatgaa
     1501 gtagttccct atatggtttc acttggttat ggattccata agataagtga tattatagaa
     1561 atttggggat acatagtaag ccaggcattt gaaaacgaat atggtgttgg ggagtttacg
     1621 gtaaatgttg ataactggga tacagatgag gcctttatta taagtgggcc gtcaccagct
     1681 gaaaatctgg ctgaactctc tacctgggat tatactatta acttcaccaa tactggggaa
     1741 gaagatttag gaactgccac aatatctatc accaacccca ttgatttgta taactatgag
     1801 gaaactatag agggtaccta tattacaata gatgaaacta ttgatttcag gaatgctgag
     1861 tttacattgg attatacaga agcgattggt aatgtcaatt attccctgga ttgtagtctg
     1921 gattccagta gctatgaact ggtggaattt acagatacag atacctatta tgattatgat
     1981 gagctgtata cagaagaatg gaatacaacc gtttatgtcc ccactgtatc tacagcacag
     2041 ttagatggca ctatagaaga tgatactata actgatgaag acggagtatt tagtgatata
     2101 tatggacaga tatttggcag tacagaagat atacctgccg aaatccctgt cttagattct
     2161 gtaacaataa ataatacatt aagtgtagat tttaacaata ataatgatat tacctttaat
     2221 ggtagttttg ttgctgatgc ctttacctat agtgggaatg gtagttttga tttcattgaa
     2281 tcaccgctta tttccctgga aaatgaggaa tatcctttac tggatagcat taatctggaa
     2341 atgacaaatt ttgaaacaaa tgtttttaga atctcagggg ggctggatgt agactttacc
     2401 accaagacca ttcaggagag tgtgggttat acatatgatt tctcagtccc ggatgaaatc
     2461 acttatcagg gaacctatca ggatataagt atagaaaatg gttttaagct tgatgggaca
     2521 ctagtaatag acccctattt tgaagatttc agtatagatt taactgaaga atacatggaa
     2581 actgaagata actatttggg ggggaagatc ttactttccg gttctctgac aaatgctact
     2641 gatgctaatt tcaacctggc ctatttcaac atttaccgtg atggatatca ccatggtagt
     2701 actgagttca ggtatgaatt tggcagtggt cattatatag aaggtgaagc cagttttgga
     2761 gagggacaac cctttaaatt ccgggcggcc aatgaccaga acctatcatt aaatatgaag
     2821 gtttatgatt ggagctcagt aacccgtgtt ggtacaatta aaaactacta tgagaataag
     2881 ctgtatggag aaatatatac agatactgat ccttacgtaa attacagtga taatactacc
     2941 gaaaccctat taccttcaga ataacaaatg cagggtaata aaaaataaat attttataag
     3001 cgggcagtca ctttgttgac tgtcccttta atcaggtgat ttttatgtca aaaaatattt
     3061 gtattttcat aattattttt atatttatct gtcctggaac tttaaaggcc ggggaaatag
     3121 gtacagagat taatattttt ttagagaata atatattttc ggttagagat gggatagatt
     3181 attttattga taacagcatt gagcaagagt atttacagga tatcattcct aataactgga
     3241 acctggttta taacaatgaa ttatgttctc aaattagtaa taatatacag gctggttata
     3301 taagagaata tgaaggggaa atacttgcta aagaagatac cgtcctgttt ttaattgata
     3361 ggataaatga taaatatgat aagtataatt acagtattga ttattacagt tttagtcaat
     3421 cactggaagg tataatacta gaaaaagata taaaatatag taatgatatt tgttttacta
     3481 ttaatagtaa attattaagg ggaattagtc tgtctgttag tgaatataat ggggaggtag
     3541 aagtgggaga agaggttctt tttgatggtc ataagcagag tattcagagc catctgggtc
     3601 aggagacgga gatgtatgat gtagacttat ccagttatgg ttttagtatt ggtggtggtg
     3661 ttcaatggtt tataggttct gattactggc ttaattttca agttgaagac ataattaatt
     3721 gtgtgttctg gaatgatgtt tacactactg atattaatta taatgataca atgctaagta
     3781 tcctatccaa taagaaaggg gatgaataca gagcccaggg ggatacaggt aaagctagtt
     3841 atagagaata taccacttat ctaacccccg aatataatct ttcacttagc aataataggt
     3901 taaatatagg gctatattat tacaaaaaag catatccctt ttttagttat aaaatattga
     3961 cagataaacc cgaactaaaa ataggtttat ttgcagatta ttatagtgtg aagattaaat
     4021 acggatgctt aaaggcttta ttaagaacac gtgatctcaa ccttatggaa tcacagggta
     4081 taactgctac attaggggtt aatctgcagt tttaattagg gtacccggtg tctcccggtt
     4141 tatgtcgaaa aatgtattag gtttaagtag aggttttatc tggtctcgtt cattaccttt
     4201 acttaaggaa acctggttac tgggatttgg ggccgatact tatcctatat actttcccca
     4261 ggatgataat gttggtaaac ttataacttt tgataaaacc cgtaaaataa tggataaacc
     4321 ccataacatg tatctacggc tgcaatcaat actggagtac cttttatgtt agccttactt
     4381 gtattgttcg taatgtattt ttttcaaagt ctcaggcttt actggaattc cgaattcagg
     4441 gatatctaca caattaccgg ggtggggata ctggctgcct ttacaggata tgtagtagca
     4501 ggtctattca atgatagtat tatttctgtt gctcctgttt tctggattct gttgggaatt
     4561 ggtataagta ttaatttgaa attgaaaaca gggaagtaaa tatatattaa cctgaattat
     4621 tcatagcact ttaaaaaaag acttttttca ataaaaacat tcaaaatcag ctccaatgat
     4681 caattagatc gaataaattt atcaatttga gtcagacatt tattaattac tccaattggt
     4741 aaataatgtt
//
LOCUS       DATGCJ010000222         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_210499, whole genome shotgun sequence.
ACCESSION   DATGCJ010000222 DATGCJ010000000
VERSION     DATGCJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210499"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VKN64_09950"
     CDS             <1..373
                     /locus_tag="VKN64_09950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60605.1"
                     /translation="WYKKHKKEGYPIIVAEVDNIVVGWASLSRWSDKKGYDYTAENSV
                     YVDKANRGQGIGYNLLKKLIEKAKQKNIKNLIALITEGNQGSINLHKKFAYEKVGNLK
                     KVGHKFNQYYDVEIYQLIVSE"
     gene            691..1482
                     /locus_tag="VKN64_09955"
     CDS             691..1482
                     /locus_tag="VKN64_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA60606.1"
                     /translation="MEIPAKRWFEAVRKRSSVRNFLPAKLKDKDEKKYSQFLEEMNEM
                     YHGIRVVYVPEGFQEVAKGFIGSYGKIDGAQSYLVCLAEKNTPYKNEKIGYMGEAAVL
                     EATAMGIASCWVTGTYKKEDVAKQVEIKDKEFIKSVIALGYAKDEKKLSEKLIKTIVS
                     SGKREALAELCEGGVDENWPDWIKTALETARLAPSAINRQPWRFKVDKNEITVKLPEG
                     NGDKDSKKLDCGIAMLHLEVGALNSGVQGEWKFQLDPEIATFKKK"
     gene            1497..2063
                     /locus_tag="VKN64_09960"
     CDS             1497..2063
                     /locus_tag="VKN64_09960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60607.1"
                     /translation="MPDKQEILKQLETERLLLKPISREFKQEIYKHFSDQAVTRYMDI
                     DVLKSVQEAEEIIDFYVEKEDQEKCYRWVIIRKEDNAFLGTCGFNSWEKCRANRVEIG
                     YDLSKKYWRQGYMQEAVKRLINHGFEILNLHRIEAKVTKGNEPSCRLLEKLNFTEEGF
                     LRDYNYWKGEYISEYMYSLLEDEYRKNL"
     gene            2378..3217
                     /locus_tag="VKN64_09965"
     CDS             2378..3217
                     /locus_tag="VKN64_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA60608.1"
                     /translation="MDIKKLINLFKKPKLFERSDQKFWDDPHISKKMLEIHIDPEFDA
                     ASRTYEFIDDSIDWLIEDVLTEKDQKIMDLGCGPGFYSKGLAEAGYSVTGIDLSERSI
                     EYARTQMENEELDVEYKVQNFLTLEYEEKFDVVLLLYCELGALTNEERNKLLNVIYKA
                     LKPGGKFIFDVFTAQNRDEEDLGKSWDIAQRGFWDEKPYISLTETFFYPEADTYLNQT
                     IIVNEEEEISLYRIFEHFYNKETISEVLEQFGFINHAFYSDLTGQKYTPKSKTLAVVT
                     EKL"
BASE COUNT         1236 a          398 c          643 g          959 t
ORIGIN      
        1 ttggtataaa aaacataaaa aagaaggata tcccataata gttgcggaag ttgataatat
       61 agtagtaggt tgggcgagcc tctcacgctg gtctgataaa aaagggtatg actatacagc
      121 agaaaattca gtttatgtag ataaagcaaa ccggggtcag gggataggtt ataatttatt
      181 aaaaaagctg atagaaaagg caaaacaaaa gaatattaaa aatctcatag cattaatcac
      241 ggaaggaaat caaggcagta ttaacttaca taagaagttt gcttatgaaa aggtcggtaa
      301 cttaaagaaa gtaggtcata agttcaatca gtattatgat gtagagatct atcagttaat
      361 agttagtgag taaaggagca gatttgatag tgaataattt ttgggctggt aagctttggt
      421 tcattttgac cgctttttta ttacttttat tatcgggatt aattttaaat ttagcaatcc
      481 agacagaaat tagcagcttc tactggatga taattccttc aattttgttt gaagaaatgc
      541 tgaaaagcag ttatctgata attgctgata taaaaataat taatttcata tttattttta
      601 tattttggtt tatagtgact atctaattgt cagcatctat gtgatattta aaactataaa
      661 aaatgaagac tctaagggga ggaataacta atggaaatac ctgcaaaaag atggtttgaa
      721 gcagttcgga aaaggagttc agtgcgtaat tttttacctg caaaattaaa agataaggat
      781 gaaaaaaagt attctcaatt tttagaagaa atgaatgaga tgtaccacgg gataagagtg
      841 gtatatgtgc cagagggctt tcaggaagta gccaaaggct ttatcggttc ctatggtaag
      901 attgatgggg ctcaatcata tctggtctgc ctggctgaaa agaatacccc ttataaaaat
      961 gagaagatcg gttatatggg agaggcagct gtcttagaag cgactgcaat gggtattgcc
     1021 tcctgctggg taaccggtac ctataaaaaa gaggatgtag ccaaacaggt tgagataaaa
     1081 gataaggaat ttatcaagtc tgtaattgct ttgggttatg ccaaagatga aaagaaatta
     1141 tctgaaaaac ttattaaaac tatagtatca agcggtaaaa gagaagccct cgctgaatta
     1201 tgtgaggggg gagtggatga gaactggccg gattggataa aaacagcctt agaaacagcc
     1261 agattggcac cttccgctat aaaccgtcaa ccctggaggt ttaaagttga taagaatgaa
     1321 atcacagtta aactacctga aggtaatggt gataaggatt ccaagaagct agactgtgga
     1381 attgccatgc tccatcttga agtaggggca cttaattcag gagtacaagg ggaatggaag
     1441 ttccagctag atcctgaaat agcaacattt aagaaaaaat aaaatgaggt ggctaaatgc
     1501 ctgataaaca ggaaatattg aagcaattag aaaccgagag gttactatta aaacctattt
     1561 cacgagaatt taaacaagaa atctataaac atttttccga tcaagctgta acaagatata
     1621 tggatataga tgtattaaag tcagttcaag aagctgaaga gatcattgat ttttatgtag
     1681 aaaaagaaga tcaagaaaaa tgttatagat gggtaatcat tagaaaagaa gataatgcct
     1741 ttttaggcac ctgtggtttt aatagctggg aaaaatgcag ggctaaccgg gtcgagatcg
     1801 gttatgatct ctctaaaaaa tactggagac agggctacat gcaggaagca gtaaaaagat
     1861 taataaacca cggctttgaa atcttaaacc tgcatagaat agaagctaaa gttacaaaag
     1921 gcaatgaacc ttcctgccgt ttactggaaa aattaaattt tacagaagaa ggttttttaa
     1981 gagattataa ttactggaaa ggcgaatata taagtgaata tatgtattca ctcttagaag
     2041 atgaatatag aaaaaatctt taattaaaat taaacctctt attacttaat aaggaagtaa
     2101 taaggggttt ttttgattta aattagttgt tttaaaacaa taaagacaat atttcaaata
     2161 tcagctataa ttaaataaga aaatatattt tgaaaggggt aataattacc tgtataatta
     2221 aggtataagt aagaaaaata aaaaactacc catataatta tattatctaa tttttgaaca
     2281 aaaaagaaat ctaagtattc aatcaaaaca tcggcaatct atagatgttt tttgagcaaa
     2341 ataaataata aaatctcttt aattaaagga gctaactatg gatattaaaa aattaattaa
     2401 cctttttaag aaaccaaaat tatttgaaag aagtgaccag aaattctggg atgatcctca
     2461 tatttctaaa aagatgttag aaatacatat tgatccggaa tttgatgccg ccagcaggac
     2521 ttatgaattt attgatgatt ctattgattg gttaattgaa gatgtattaa cagaaaagga
     2581 tcaaaagata atggacttag gttgtggccc tggtttttat tccaaggggt tagcagaagc
     2641 aggttattca gtaacaggca tagatttatc agaaagatca atagagtatg cgagaactca
     2701 aatggagaat gaagaattag atgtggaata taaagtgcag aactttttaa cacttgaata
     2761 tgaagaaaaa tttgatgtcg tattacttct gtattgtgaa ctaggggctt taactaatga
     2821 agaacgtaat aagttactta atgttattta caaagcttta aaaccaggcg gcaaatttat
     2881 ctttgatgtc tttacggccc aaaataggga tgaagaggat cttggtaaaa gttgggatat
     2941 agctcaaaga ggtttctggg atgaaaaacc ttacatatct ttaacagaaa catttttcta
     3001 tccggaggct gatacatatt taaatcagac tattatagtt aatgaagaag aagagatatc
     3061 tttatatcgg atatttgaac atttctataa caaagaaact attagtgagg tattagagca
     3121 atttggtttt attaaccatg ctttttattc tgatttgacc ggccaaaaat atactcctaa
     3181 atctaaaact ttagctgtag taaccgaaaa actttaatta taataataaa taagat
//
LOCUS       DATGCJ010000223         6759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_391563, whole genome shotgun sequence.
ACCESSION   DATGCJ010000223 DATGCJ010000000
VERSION     DATGCJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391563"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1288)
                     /locus_tag="VKN64_09970"
     CDS             complement(65..1288)
                     /locus_tag="VKN64_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60609.1"
                     /translation="MVDIVLTTNSPGEVASWVKPVVRRLNELNIEKNIYVFTPPCVFS
                     SGNEGKVLSKLEGITAAFNSRQYLKYILLNIKPDNFKPSKDGFVFFLGGDFMHAVFLG
                     KRLDYPVYAYTERDYGFPKSIKKYYLSDQNLYNKMKKDGIDEDKLKIVGNLMFDSINP
                     QLTQKATAKLLNKKEGETLINLFPGSRPKEFSYLLPLYLNFISEINKKENNYRFIISK
                     AAFINERDIEMVIRDERIKEQVTYEPEKNTIILNGCKIKIYEENLQSIMRESDFAVTI
                     PGTNNLELAVLDTPMLVILPLNKPELIPLPGIIGLIGELPIIGKIIKKSISSKVLKKR
                     EYFSLVNILSQKEIVPSLVGEMDSKQLSQVVTELLREEKFNELKAKIKSFEGQKNAVN
                     KIVEDILKDLGNVNI"
     gene            complement(1304..1912)
                     /gene="cobC"
                     /locus_tag="VKN64_09975"
     CDS             complement(1304..1912)
                     /gene="cobC"
                     /locus_tag="VKN64_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HMA60610.1"
                     /translation="MTTKIVLARHGETEWNKTSRYQGVRDIELNEKGQRQAEKLFDFL
                     KDEAFDTIYSSTLKRAQETVQGIANYQNKEIITISELMEIDFGQWEGLTFSEIEENYP
                     DLAKKWAKDPTCCKPPDGEHIKEVEERVGETIDKIVDDNPDKKILIATHGGIIRIIFA
                     YLLDLPLSRIFSIEVENVSISRIHFYKHYPVVNLLNSIHHLK"
     gene            complement(2060..3007)
                     /locus_tag="VKN64_09980"
     CDS             complement(2060..3007)
                     /locus_tag="VKN64_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HMA60611.1"
                     /translation="MRFKKSINLITLFLILSLLIISGGAIAQEFPVTITDGLGEKVVI
                     EEKPAKIISISPQTTENLFAVRAGEQVIGVTTYADYPKEATKVDKIGTITEPNIEKIV
                     SMEPDLVIAASVNKMETIERLRELNIKVAGFKASSVNTAIENIKKIGKITGNQDIAEE
                     VTADMYIKISEMKDLIDKKLANNDRPKVFYELWSDPLYTAGVDNFIDDLIHMAGGFNI
                     GRLAETQWPQFSLEKLLVEDPEVYISTPHSAEMQVSKESIKNRKRFKDITAIKNDRVY
                     IIDQNIINRASPRLLIGLSQLTKAIWPDLEDEVNEIMEK"
     gene            complement(3037..4110)
                     /gene="cobD"
                     /locus_tag="VKN64_09985"
     CDS             complement(3037..4110)
                     /gene="cobD"
                     /locus_tag="VKN64_09985"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HMA60612.1"
                     /translation="MEQAHGGNLKEAAEKFNISESEIIDFSANINFVGPPPDIYSKIR
                     KEIEAITRYPEPDSTSLKKLLAEKYGGKADNYVLGNGAVELIYILTRILDLNKGLVLA
                     PTFSEYEKALKAEEIEVNYHYISTDHSFKLNINRLKKDLTGDIDIFFLCNPNNPTGKF
                     ITRDKIKELLLHNKKKDIFTVIDEAFVDFLEQKITSLSFLKDFDKLFILRSLTKFYAV
                     PGLRLGYGIANKEIIKKINKAKDPWNVNSLAQKAGELILEQKRYDKLTKKAIYREKIF
                     LYQELKKFKNLRVYYPNANYIFIDLTDSNYSGSELYNQLAERSILIRNCSNYKGLDDN
                     FIRVAVKSREENKILLKALKSFL"
     gene            complement(4115..4999)
                     /locus_tag="VKN64_09990"
     CDS             complement(4115..4999)
                     /locus_tag="VKN64_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HMA60613.1"
                     /translation="MSYTVKVPGSCGELAQGYLNNSNFLISCPIDIYSEVTVEINKEN
                     DEIIIEESKNKTKEALNILRDYYDLKNIGLQVKINSKLPRSKGLGSSTADMSAALIAA
                     VKVLDLELDLNQIKEILLKVEPSDATFLKGINLFDHIEGSMIRFLGNIEPIPLLVFDY
                     GGKVDTLAFNTRVDLDNLNRGKSKQIEKAYNLISKGVKNNNKEMIGKGATISSLANQI
                     ILKKTGLERLVKGLEQKVGFLGVNIAHSGTVLGIMVKDPLYFDDIINFVGSRAPQLKL
                     LFKTKIVNGGYKTIKNEG"
     gene            complement(5015..5749)
                     /gene="cobS"
                     /locus_tag="VKN64_09995"
     CDS             complement(5015..5749)
                     /gene="cobS"
                     /locus_tag="VKN64_09995"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HMA60614.1"
                     /translation="MNSFLIALQFITIIPVKKELDYREKNIAKSMLYYPLIGTIIGLF
                     LVLVNIIGTIFLPDLVRDSLLLIFFVLFTGGIHFDGLADTFDGLFGGSNKKRILEIMG
                     DSSIGVYGILAVVLVLILKLSLLAELPMSVRNLALITAPTISRCAMVWAVYLFPYAKK
                     EGFGKAFKLYLTRNEAWLTAVYTVIVTLALFLFKGIIILVISALGIWLFGTFIVKKIE
                     GLTGDNYGALNELMEVLVLFLIILLY"
     gene            complement(5762..>6759)
                     /gene="cobT"
                     /locus_tag="VKN64_10000"
     CDS             complement(5762..>6759)
                     /gene="cobT"
                     /locus_tag="VKN64_10000"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HMA60615.1"
                     /translation="KEAMEKAKERLDNLTKPPGSLGYLEELAIKMAGITKDIFPDIAQ
                     KEHVIMVGDHGVVKEGVSAFPQTITKAMVENFLNGGAAVNVLARQQGVNITIVDIGMV
                     NQVSKEGILQANIKKGTDNLLEGPAMSESEAVASIETGIEITNKLIEQGAKVIGTGEM
                     GIGNTTPSSAIIKAATDMDIDEVVGYGTGLDEEGLDHKKEIIKRAISVNKPNRDDGID
                     LLSKVGGLEIGGMAGVMLAGAKARKPVLIDGLISAAAALIAYLIEPKVVNFLIPSHKS
                     VEPGHHKLYQFLDMRPMFDLDMRLGEGTGAILGMGIVESANRIIKEMATFDTLDF"
BASE COUNT         1997 a         1359 c          920 g         2483 t
ORIGIN      
        1 atctgcattt aagcttctcc tccacattaa ttaaattata ttttttacct aatatttata
       61 ttgtttaaat attaacattc cctaaatctt ttaaaatgtc ttccactatt ttatttacag
      121 catttttctg tccctcaaaa ctttttatct ttgcttttag ttcattaaac ttttcctctc
      181 ttaaaagctc tgttacaact tgtgataatt gtttgctgtc catctcaccc actagggaag
      241 gaacaatttc tttttgggat aaaatattta ctaaagaaaa atattctctt ttctttaata
      301 ctttagagga aatacttttc ttaattattt ttcctattat tggtaattca ccaattaatc
      361 ctatgatacc aggtaaaggg atcagttcag gtttattaag aggtaaaatt actaacatag
      421 gagtatccaa aactgctaac tcaagattat ttgttccagg tatggtaact gcaaaatcag
      481 attccctcat tatactctgc aaattttctt catatatttt tattttacaa ccgtttaaaa
      541 taattgtgtt tttctctggc tcataagtaa cttgctcttt gatcctttca tcccttatta
      601 ccatttcaat atctctttca ttaataaaag cagctttact tattataaag cgataattat
      661 tttccttttt atttatttct gaaataaagt ttaaatataa gggaaggaga taactaaatt
      721 ctttaggcct actgcctgga aataaattta ttaaggtctc tccttctttc ttgtttaaca
      781 atttagctgt cgctttttgg gttaattggg ggttaatgga atcaaacata agattaccca
      841 ctatttttag tttgtcctca tcgattccat ccttcttcat tttattatat aaattttgat
      901 cagaaagata atattttttt atacttttgg gaaaaccata gtccctttct gtataagcat
      961 aaaccggata atctaatctt ttacccaaaa acacggcatg catgaagtct cctcctaaaa
     1021 agaacacaaa accatcttta gaaggtttaa aattatcagg tttaatattt aatagtatat
     1081 attttaaata ttgcctgctg ttaaaggcag ctgttatacc ctctaattta gataaaactt
     1141 taccttcatt cccgctcgaa aaaacacagg gaggggtaaa tacatagata ttcttctcta
     1201 tatttaattc atttaatctt ctaaccaccg gttttaccca ggaagcaact tctccagggc
     1261 tattggtagt taaaacgata tcaaccacaa aaaccagcct cccttatttt aaatggtgaa
     1321 tactatttaa taaattaaca acggggtaat gtttgtaaaa atgaatacgt gatatagaca
     1381 cattttcgac ttcaatacta aagattctac ttaagggtaa gtccaataaa taggcaaata
     1441 taattctaat aataccacca tgggtggcta tcaagatttt tttatcagga ttatcatcaa
     1501 caattttatc aattgtctct cccacccttt cttctacctc ttttatatgc tcgccatctg
     1561 gtggtttgca acaggtgggg tcctttgccc atttctttgc caaatcaggg taattttctt
     1621 ctatttcaga aaaagttaat ccttcccact gaccgaaatc tatttccatc agttcagaaa
     1681 tagtaataat ttccttattt tgataatttg caatcccttg cacagtttcc tgagcccttt
     1741 tcaaggtact actgtatata gtatcaaacg cctcatcttt tagaaaatca aataactttt
     1801 ctgcctgtct ttggcctttt tcatttaact ctatatccct aactccctga tagcgggaag
     1861 tcttattcca ttcggtctct ccatgtctgg ccaacactat tttagttgtc atagttacac
     1921 cttcttattt tagttttctt ttattattat aattgaaatt ttattctata tcaattaaag
     1981 tagtgtttta acttctcaaa ttattaaaac ccccacaaga aatcgtgagg gtattaatag
     2041 tcttaattgt catttttatc tacttttcca taatctcatt tacttcatct tctaaatcag
     2101 gccaaattgc tttagtcaac tgagataatc caattaatag tcttggtgaa gcccggttta
     2161 taatattttg atctataata taaaccctgt catttttaat ggctgttata tctttaaatc
     2221 tttttctatt ttttatactt tcttttgaaa cctgcatctc agcactgtgg ggtgttgaaa
     2281 tatatacttc tggatcttct accagcagtt tttctaagct aaactgcggc cattgagttt
     2341 cagccagacg acctatatta aatcccccgg ccatatggat aagatcatca ataaagttgt
     2401 ctaccccagc agtgtatagg ggatcgctcc acaattcata aaaaactttc ggcctgtcat
     2461 tattagctaa ctttttatca attaaatctt tcatctcgga aatctttata tacatatcag
     2521 ctgtaacttc ctcagcaata tcctgattac ctgtaatctt accgatcttt tttatatttt
     2581 cgatagcagt attcacacta cttgctttaa aacctgctac cttaatattt aattctctta
     2641 atctctcaat ggtttccatc ttattgacag aggcagctat tacaaggtcg ggttccatgg
     2701 aaactatttt ctcaatgttg ggttctgtaa tggtaccaat tttatctact ttggtcgctt
     2761 ctttagggta atcggcatag gtggtaactc ctattacctg ctctcccgcc ctaacagcaa
     2821 agagattttc agtagtctga ggtgaaattg aaatgatttt ggcgggtttt tcttctatca
     2881 ccactttttc acctagaccg tctgtgatag taaccggaaa ttcctgagct atggcccctc
     2941 cactaatgat aagtaaactt agaattaaaa ataatgtgat tagattgata ctctttttga
     3001 atctcatttt attcctcctc ttatattttt atgcttttat aaaaaacttt ttaaagcttt
     3061 taataatatt ttgttttctt ctctactttt tactgcaact ctgataaagt tgtcatctaa
     3121 tcctttataa ttgctgcagt tgcggatcaa aatagatctc tctgctaact gattgtaaag
     3181 ttcagatcca ctataattac tatctgttaa gtcaataaag atataattgg cattgggata
     3241 gtacaccctt aaatttttaa attttttaag ttcctgatag agaaatattt tttctctgta
     3301 aattgctttt tttgttaact tatcataccg tttttgttct aatattagtt ccccggcttt
     3361 ttgggccagg ctatttacat tccaggggtc tttagcttta ttgatctttt tgataatctc
     3421 cttatttgct attccataac ctaatcttaa gccgggtacc gcataaaact tggttaaaga
     3481 ccttaaaata aagagtttat caaaatcttt taaaaaactt aaggaagtta tcttctgttc
     3541 tagaaaatca acaaaggctt catcaataac agtaaaaata tctttctttt tattgtgtaa
     3601 taatagttct tttattttat ccctggtaat aaatttaccg gtagggttat taggattaca
     3661 taaaaagaag atatcaatat ctccagttag atcttttttt aagcgattta tatttaattt
     3721 aaaactatgg tcagttgaaa tgtaatgata attaacttct atttcttctg cttttaatgc
     3781 tttttcatat tcagaaaagg tgggcgctaa aactagacct ttatttaaat ctaatatcct
     3841 ggttaagata taaattagtt caactgcccc atttcccaat acataattat ctgccttccc
     3901 accatatttt tcggctaaaa gtttttttaa ggaagtagaa tcaggttcgg gataacgagt
     3961 aatagcttct atttcctttc tgatcttgct atagatatca ggtgggggac ctacaaagtt
     4021 tatattggca ctaaagtcta ttatttcact ttcactaata ttaaattttt cagctgcttc
     4081 tttgagattg ccgccgtgtg cttgttccat acttttaacc ctcattttta atagttttat
     4141 aaccaccgtt tactatcttg gttttaaata ataattttag ctgaggtgct cttgatccta
     4201 caaaatttat aatatcatca aaatataagg ggtcttttac cataattcca aggaccgtac
     4261 cactgtgcgc tatgttaact cctaaaaagc ccactttttg ttctagcccc ttaactaacc
     4321 tttccaaacc tgtcttcttt aagataatct ggttggctaa actacttata gtagcccctt
     4381 taccgatcat ctctttatta ttatttttaa cacctttact tattaaatta tatgcttttt
     4441 caatttgttt actcttacct cgatttaaat tatccaggtc tactctcgta ttaaaagcca
     4501 gggtatccac tttccctcca taatcaaata ccaataaagg tattggctcg atatttccta
     4561 aaaatcgaat cattgaacct tcaatgtggt caaataagtt tatacccttt aaaaaggtgg
     4621 catcagatgg ttctactttt aataaaattt cttttatttg attcaaatcc aactctaaat
     4681 ctaatacttt cacagctgct attaaggcag cactcatatc agcagtagaa ctacccagac
     4741 ccttagaacg gggtaatttt gaatttatct taacttgcaa tcctatattc tttagatcat
     4801 aataatccct tagaatattt agggcttctt tcgttttgtt tttactttct tctataataa
     4861 tttcatcatt ttctttattt atctcaacag tgacctcact atagatatca ataggacagc
     4921 ttattaaaaa attactgttg ttcagatagc cctgggccaa ctctccacaa ctgcccggta
     4981 cttttactgt ataactcata aatttgctcc ttaattaata caataatatt attaaaaata
     5041 agactaaaac ttccattaat tcattgagtg ctccataatt atcgcccgtc aaaccctcta
     5101 tcttctttac aataaaagtc ccaaaaagcc agattcctaa agcagatatg actaaaatta
     5161 taattccctt gaagagaaat agtgctaaag tgactattac tgtatagacc gcggtaagcc
     5221 aggcttcatt tcttgttaaa tatagtttaa atgctttacc aaatccttcc tttttagcat
     5281 aggggaatag atagactgcc caaaccatgg cacagcggct gatcgttggt gcagtaatca
     5341 aggccagatt ccttacactc atcggtaatt cagccagcaa acttaattta agtattaata
     5401 ctaagacaac cgccaggatc ccatagaccc cgatactgct gtctcccata atttctaaaa
     5461 tccttttttt gttactgccg ccaaaaagtc cgtcaaaggt atcggccaaa ccatcaaaat
     5521 ggataccccc ggtaaataag acgaaaaata ttaaaagcaa actatcccgg acaagatcgg
     5581 gtagaaaaat agtacctatt atattaacca gtactaaaaa tagaccaata atcgtcccaa
     5641 tcaggggata gtaaagcata gatttcgcta tgtttttctc cctataatca agttcttttt
     5701 ttaccgggat gatggtaatg aactgtaagg caattaaaaa tgagttcatc ttaaacctcc
     5761 tctaaaaatc gagtgtatca aaggtagcca tctctttaat tatccggttg gcagattcaa
     5821 ctatacccat tcctaaaatt gctcctgtac cttcacccag cctcatatct aaatcaaaca
     5881 tcggcctcat atccaggaat tgatataatt tatgatggcc gggttctacc gatttatggg
     5941 aagggattaa aaagttaact actttcggtt caattaaata ggcaattagg gcggctgcgg
     6001 cagaaataag cccatcaatt aaaaccggtt ttctggcttt agcacctgct aacattactc
     6061 cggccatacc gccgatctct aaacctccca ccttacttaa gagatcaatt ccatcatctc
     6121 tgttcggctt atttactgaa atagcccttt ttattatctc ttttttatgg tctagacctt
     6181 cctcatcaag gcctgtacca taacccacaa cctcatcaat atccatatcg gtagcagctt
     6241 tgattattgc gctactgggt gtagtattac cgattcccat ttcaccggtt ccaattactt
     6301 ttgctccttg ttcaattaac ttattggtta tctcgattcc ggtttcaatg cttgctaccg
     6361 cttcactctc actcatggcc ggcccttcta acaagttatc tgtacccttt ttaatattag
     6421 cctgcaggat accttcttta ctaacttgat tgaccatccc gatgtctaca atagtaatat
     6481 ttactccctg ctggcgggct aaaacattaa cagctgcccc tccgttaaga aagttttcta
     6541 ccattgcttt tgtaattgtc tggggaaaag cactgactcc ttctttgaca acaccgtggt
     6601 cccccaccat tattacatgt tctttctgag ctatatcggg aaagatatct tttgtaatac
     6661 cggccatttt gattgctaat tcctctaaat agcccagact gccgggcggt ttagtaaggt
     6721 tatctaatct ttctttcgcc ttttccatcg cctctttat
//
LOCUS       DATGCJ010000224         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_435931, whole genome shotgun sequence.
ACCESSION   DATGCJ010000224 DATGCJ010000000
VERSION     DATGCJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435931"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..432)
                     /locus_tag="VKN64_10005"
     CDS             complement(223..432)
                     /locus_tag="VKN64_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HMA60616.1"
                     /translation="MIYTGTVKWFDAKKGYGFIEREEGDDVFVHFSAIQDDGFKTLED
                     GEKVEFEIVEGDRGPQAAQVVRVEE"
     gene            complement(568..1197)
                     /locus_tag="VKN64_10010"
     CDS             complement(568..1197)
                     /locus_tag="VKN64_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMA60617.1"
                     /translation="MFDQKQGEEEIIKEAKEGNKLAIEYLINQNMDIVYAKAKYFFIK
                     GLDKEDVIQEGMLGLFKAIRDYQPDREASFRGFSQLCVHRQLISAIKRANRQKHQPLN
                     NSTSINKTYDYDNESGRSFSEILPDKKKKLEDQFVYKEIINLVFQEIERELTELEKNV
                     FYEYLDDRTYKEISDKLEINVKTVDNALQRARKKIEEIKANNNLEDLVI"
     gene            complement(1224..1364)
                     /locus_tag="VKN64_10015"
     CDS             complement(1224..1364)
                     /locus_tag="VKN64_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60618.1"
                     /translation="MAKKKKEEEKRNKYLDIILVLILLLVLATFIDLNVTIEEVFRIL
                     IG"
     gene            complement(1390..1911)
                     /locus_tag="VKN64_10020"
     CDS             complement(1390..1911)
                     /locus_tag="VKN64_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60619.1"
                     /translation="MYKKVLILILLIAFSLTPIANAQSHDFQEMNYSVFLKGDNSSLN
                     INPVFQFQNEINHLSFDINYDGDFFRLGGTWLITALEETSYRTDAAFSILTEPNGEGF
                     TAGLGVEGKNYLEENRALLYELRYILDGTQFEYRLGYETPIVENNALLLSIGNSYWHN
                     TALLLEVGFKVQF"
     gene            complement(1927..3960)
                     /locus_tag="VKN64_10025"
     CDS             complement(1927..3960)
                     /locus_tag="VKN64_10025"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HMA60620.1"
                     /translation="MKTKNNVVIIVLLTFLLILVPHQVNAFSNALKNFVSNGKEVHLK
                     IFEDLDQAEFLSGTNVELKNLDGSKRTFLSKNKEYIISPYREDQKSWFIQVFATSEKN
                     KAENMKENIKKEFENYEVQISLEDDLYKVQVGTFLKESKAEEISQIISEAGWETWVIE
                     KKIKGDSTELAVMDKDGNIVFENGTIYCEGVIRLKNNLYNGMIQFSLTDGEIDVFNKV
                     DFLTLQYGMLLEELPNSSNMTALKAAAILNRSKALAVIFNNNSPYQIESYQGISNLTD
                     TIRIAVDKTEAKVIYRDDNPYYKSLNVKELGSQYSTAEELITQNIASIKIKDLATTID
                     EEQLVRAEIDLGLEYREIRQLTWSGPRIITVVDLDLFKGRHQVIPFVSNDEIPGLAPL
                     VKVVREKEALVGVNGGFYNSEGKPLGTVMIDGQMVSEPLYNRAALGITKTGEVLINNL
                     KWRGILTTSNNNNIVIDAVNRKPNNENEIILYNKYYGSKTPQFKQQSVEFIIQNNRIS
                     KVDSGSMIQNEIPTDGFVVKIFTEENTFNAFKQGEKVEYKNIFTPAWHKEDVVNIMGG
                     GPKLITDGKVTVTGKLEEFSQDITTGRAPRTAVGITEDNHLLFVTVDGRQPKLSIGIT
                     LEELAKYLKELKMEEAMNLDGGDSSQLVIRGYTFNNPSGYRAIATGILIKKRE"
     gene            complement(3983..4261)
                     /locus_tag="VKN64_10030"
     CDS             complement(3983..4261)
                     /locus_tag="VKN64_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HMA60621.1"
                     /translation="MENGTQGMSINFSRHAQSRITSREEIEITDFEMSQLKRGIEKAL
                     EKGAKNSLIIINKTAFIVSIENKTVITILDQDSIKEKVFTNIDSAVLM"
     gene            complement(4279..4809)
                     /locus_tag="VKN64_10035"
     CDS             complement(4279..4809)
                     /locus_tag="VKN64_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60622.1"
                     /translation="MKKYLYTFLIVIIIIGSLLLINNLGIISFRQWGENIIQNTPFVK
                     EYVRTAEEYEEVVEENQKLQEANEDLKGKVSSQEEKIAELEEQIKELEEANKELTTQL
                     EEIQQKEESKQANFEKLLKIYENMNPESAADILNSLEREYALKILKGLNDETAAAILE
                     SMEVEKAVEFSTELQN"
     gene            complement(5004..5327)
                     /locus_tag="VKN64_10040"
     CDS             complement(5004..5327)
                     /locus_tag="VKN64_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMA60623.1"
                     /translation="MINNVILTGRLVKEPELKKSANEVDYCTFTLAVQRNYRSKEGEI
                     DTDFIDVINWGPGAKRCSEYLKKGKLAGVEGMISTYSVEKEGKKYKNYRIKARRVEFL
                     EWPDK"
     gene            5579..>7696
                     /locus_tag="VKN64_10045"
     CDS             5579..>7696
                     /locus_tag="VKN64_10045"
                     /inference="COORDINATES: protein motif:HMM:NF023351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HMA60624.1"
                     /translation="MNYRKKATYLFILLALTLVVSFQVSAAEYNRQPDFNLYGSFGDQ
                     FTGQASLLYPFNNKEDSLFYTDLRGMIGSNDISEWNLGFGYRKKIQDRDFILGTYLFK
                     DNRKEFNRDWNQWTIGGELLTKNMDFRFNYYLPEKDRVLASSLPNDSVKVVGNSLVYE
                     KAQLNTYYEVMGGYDLEFGKRFTETEGYLKNFGTFFRVYNFAGDDTITMSGIGVKIDK
                     TFGDPNDVNYKLGVDWQNDNIRGSSTEATFEISIPLGKSSKSSIDKPNDELENRMTEK
                     ARRDIDVIIGETKPERVLEAKEVAQDTDGDNTFIPAGKVWYVSANGDGNGSKDDPINL
                     KDLFAPVNAESIELNRAGNNTNIAEEGDIIILLGNEGDFLLTPEEINSLRLEPRQKLI
                     SPGGYLKIYSESDQTRYQYFKPEGKTATIKSDNSIEQVNTQLRIGIADMVQLTNNNTV
                     SGINFVGASDEALFGNGEAVVGDNVTGKINITNNTFSNLETAINIKGFKGESKINIKN
                     NRLDNVAFGISVGNEDIFRGGPIMPANTNQSVEFTSPLIHMQSEIGDIKVTISENIIN
                     DSLFYGILTNLDRSTNHSITIDNNEIDLRDLNEVMIEEDGFISPMPPFMMGNGIFVNT
                     DNAINAEVKIYKNKIKYAYQDGILVTSWNPRETNDIQIYKNQIGKVGSPYIDYYDYEA
                     NLTDFSSPSEYDLVNNGITVAVLN"
BASE COUNT         2427 a         1425 c         1111 g         2733 t
ORIGIN      
        1 ttcgatcttt tctcgggcat aatcttttag tgaaggtgtt acgtcgatat tttttccata
       61 aaccgtaata ttcattatct ccactccctt ctggactact aaaataaata ttggtgttat
      121 tataataatt caatatattg atttaaaacc cttctttgta taaaaaaaat cctgatcaca
      181 tttctgcaat caggagtgtg tgcacatatt aggcatctat aattattctt caaccctcac
      241 aacttgtgca gcttggggtc ctctgtcacc ctctacaatc tcgaactcta ctttctctcc
      301 atcttcaaga gttttaaaac catcatcttg aatagctgag aaatgaacaa aaacgtcgtc
      361 gccttcttct ctttcaataa agccataacc tttttttgcg tcaaaccact taactgttcc
      421 agtatagatc ataaattata ttcctcctgt aaattgtttc tggggaaata aaaattccca
      481 tttatcttaa tataacacag tatattatag ttgtcaaaca agttattata aaagatatca
      541 acttggtttt agatacaata tactcactta aattacaagg tcttctaaat tattattagc
      601 cttgatttcc tctatttttt tacgcgctct ctgtaatgca ttatcaacgg tctttacatt
      661 tatctccaac ttgtcagata tttctttata agtgcggtca tctaagtatt cataaaaaac
      721 atttttttct aactcagtta actctctttc aatctcctga aatactaaat ttataatttc
      781 tttataaaca aattgatctt ccaatttttt cttcttatcg ggtagaattt cactaaaact
      841 tctccccgac tcattatcat agtcataagt cttattaata gaagtggaat tatttaaagg
      901 ctggtgtttt tgtctgttag cccttttaat ggctgagata agctgtctat gcacacataa
      961 ttgagaaaac cctctaaaag aagcctcgcg gtccggttga tagtccctga tcgccttaaa
     1021 tagtccgagc atcccttcct ggattacatc ctctttatca aggcctttga tgaaaaaata
     1081 cttcgccttc gcataaacga tatccatatt ctgatttatt aaatattcaa ttgctagctt
     1141 atttccttct tttgcttctt taattatctc ttcttctccc tgcttctggt caaacattca
     1201 atccccccca atttgaaaat gctttaacca attaaaatcc taaatacttc ttctattgtt
     1261 acattcaaat ctataaaagt cgctaatacc aatagtagga ttaatactaa aataatatct
     1321 aaatatttat ttctcttttc ctcctctttt ttctttttag ccatagttaa ctactccctg
     1381 ctctatatat taaaattgga ctttaaatcc aacctctaat aaaagtgctg tattatgcca
     1441 gtatgagtta ccaatactaa gcagcaaggc attattttca actattggtg tttcatagcc
     1501 tagcctatat tcaaactggg ttccatccag gatatatctc agctcataaa gtaaagctct
     1561 attttcttca agataatttt taccttcaac tcccaagccg gctgtaaacc cttctccatt
     1621 gggctctgtt agaatactaa aagcagcatc agttctataa ctggtttctt ctaaggctgt
     1681 aattagccag gtaccaccca atcggaaaaa atcaccatca taattaatat caaaacttaa
     1741 gtgattgatt tcattttgaa attgaaaaac aggatttata ttaagagatg aattatctcc
     1801 ttttagaaat acagaatagt tcatttcctg aaaatcatga ctttgtgcat tggctattgg
     1861 tgtaagtgag aaagcgatta atagaattaa gattagaact tttttataca agtttatcaa
     1921 cctccattat tctcgttttt ttattaagat tccggttgca atggcccgat aaccagaagg
     1981 attattaaag gtatatcccc tgattacaag ttgggaagag tcgcctccat ctaggttcat
     2041 agcttcttcc attttcaact cttttaaata tttagctaat tcttctaagg tgatccctat
     2101 acttaatttc ggctggcgtc catccacagt tacaaataat aaatgattat cttcagttat
     2161 accaactgct gtccgaggtg ctctgccagt tgttatatcc tgactgaact cttctaattt
     2221 acctgtaact gtgacttttc catccgttat taatttaggt cctcccccca tgatattgac
     2281 tacatcttct ttatgccagg ctggagtaaa tatattttta tattctactt tttcaccttg
     2341 tttaaaagca ttaaaggtgt tttcttctgt aaatatttta actacaaatc catcggtagg
     2401 aatttcattt tggatcattg aaccactgtc tactttacta atcctattat tttgaatgat
     2461 aaattcaact gattgctgtt taaattgagg ggttttactg ccataatatt tgttgtataa
     2521 aattatttcg ttttcattat ttggttttct gtttaccgca tcaataacaa tattattatt
     2581 gttagaagtg gttaatatcc ctctccattt aaggttatta attaatactt caccagtctt
     2641 ggttattccc agggccgccc tgttatagag cggttcagaa accatttgtc cgtcaatcat
     2701 aactgtaccc aggggtttac cttcggaatt ataaaaaccg ccgttaactc cgacgagggc
     2761 ttctttttct ctaaccactt ttactaaagg ggctagacct ggaatttcgt cattacttac
     2821 aaaaggtata acctgatgtc ttcccttgaa taaatccaaa tcaaccactg ttataattct
     2881 gggacctgac caggtaagct gtctaatctc cctatattca agacccaggt caatttcagc
     2941 tctcacgagc tgttcttcgt ctatggtagt agcaagatct tttattttaa tactagcaat
     3001 attttgtgta attaactcct cagctgtaga atattgacta cctaattcct taacatttaa
     3061 acttttataa tagggattat catctctata aatcactttt gcttctgtct tatcaaccgc
     3121 aattctaata gtatcagtca gatttgaaat gccctgataa ctctctattt gatagggcga
     3181 attattatta aatattacag ccagggcttt actcctattt aaaatagcgg ctgctttcaa
     3241 ggctgtcata ttgcttgaat tgggcagttc ttctaaaagc atgccatact gaagagttaa
     3301 aaaatcaact ttattaaata catcaatctc gccatctgtt aaagaaaact gaatcatgcc
     3361 attatataag ttattcttca acctaataac gccttcacaa taaatggtcc cattttcaaa
     3421 gacaatgttc ccatctttat ccataacagc tagttctgtg ctatctcctt ttatcttttt
     3481 ttcgataacc caggtttccc aacctgcctc acttattatc tgtgatatct cttctgcttt
     3541 actctctttt aaaaaggtac caacctggac tttataaaga tcatcttcta aactgatctg
     3601 tacttcataa ttttcaaact ctttttttat attttctttc atgttttctg ctttgttttt
     3661 ttcggaagta gcaaagacct ggataaacca acttttttga tcttctctat agggacttat
     3721 tatatactct ttgttttttg ataaaaaggt tcgtttggaa ccatcaaggt ttttcagttc
     3781 aacattagta cccgataaaa attccgcctg atctaaatct tcgaatattt ttaagtgaac
     3841 ctctttacca ttagaaacaa aattttttaa ggcattagaa aatgcattta cctggtgggg
     3901 aactaatatc agaagaaaag ttaataaaac tataattacc acattatttt tggttttcaa
     3961 atacatcact ccacccttac tatcacatta aaaccgcact atcgatattt gtaaaaactt
     4021 tttcttttat actatcctga tcaagtatgg tgatgacagt cttattttct atgctgacta
     4081 taaaagcagt tttattgatg attattaatg agtttttagc ccctttttca agagcttttt
     4141 caattcctct tttcagttgg gacatttcaa aatctgtaat ctctatctcc tcgcgtgagg
     4201 taatcctgct ttgtgcatga cgggagaaat taatgctcat accttgagta ccattttcca
     4261 ttccatcacc tcaaagaatc aattttgcag ttctgtacta aactccacag ccttttccac
     4321 ttccatactt tccaagatag cagcggcagt ttcatcgttt agacctttca aaatttttaa
     4381 cgcatattct ctttctaggc tgtttaatat atctgcggcg gactcgggat tcatgttctc
     4441 atatattttg agcagttttt caaagttagc ctgtttagat tcttcttttt gttgtatttc
     4501 ttctaattga gtagttaatt ctttattagc ttcctctaat tcctttatct gttcttctag
     4561 ttcagcaatt ttttcctctt ggctgcttac ttttcctttg aggtcttcat tggcttcctg
     4621 tagtttttga ttttcttcaa ctacttcctc atactcttcc gccgttctca catattcctt
     4681 aacaaaaggc gtattttgta tgatattttc tccccactgc ctaaaagaaa tgattcccaa
     4741 attatttatt aataatagag aaccaattat aataataaca attaaaaaag tataaagata
     4801 ttttttcaaa ctttctcccc ccattttgtt taaaaatatc tatcattatt cctatctcga
     4861 tctataaaaa agttttaatt tatgcaaaaa atagataatc gtgttcatta cttaatatta
     4921 ttcaacaaaa aaattccaat tcctccctta ttagtcagta aaaaaagggg ttaatgaaaa
     4981 aattcattaa ccccttaaaa atattattta tctggccatt ctaaaaattc aactcttctt
     5041 gctttaatcc ggtaattttt atattttttt ccttcttttt caaccgagta tgtacttatc
     5101 atgccctcta caccggctaa tttacccttt ttgaggtatt cactgcatct tttagcgccc
     5161 ggaccccagt taataacatc gataaagtcc gtgtcgatct ctccctcttt actccggtaa
     5221 tttctctgta ccgcaagagt gaaagtacaa tagtctactt cattggctga tttctttagc
     5281 tcgggttctt ttaccaatct acctgtgagt attacgttgt ttatcatatc accactcctt
     5341 ttccatatcc taccatatat aaatgttatt aacaagaaac taagttaaat atttccattt
     5401 aattgttttt tataagaaaa ctatattcat atcctgatat atatgtatat ttttcggtaa
     5461 attatgcagg aattatagag tcagtcctta attaatagat atatttttat atttttgcat
     5521 ataatgtaat tttaagcaaa aactataaaa ataaatacta atataatagg aggaacaaat
     5581 gaattacaga aaaaaagcaa catatttatt catcttattg gcattaaccc tggtagtttc
     5641 atttcaagta tctgcagccg aatacaacag acaacctgac tttaatcttt acggcagttt
     5701 cggtgatcaa tttaccggac aagccagcct cttatatccc ttcaataaca aagaagattc
     5761 acttttttat acagacctaa gagggatgat tggcagtaat gatatctcag aatggaatct
     5821 tggttttggt tatagaaaga aaatccagga cagagacttt attttaggta cctatttatt
     5881 taaagacaat cgtaaagaat ttaacaggga ttggaaccaa tggactattg gtggagaact
     5941 tctaacaaag aatatggact tcaggttcaa ttattatctg cccgaaaaag atagagtctt
     6001 agcctctagc ttacccaatg actccgttaa agtggttgga aacagtcttg tatatgaaaa
     6061 agcacaacta aacacctatt atgaagttat gggtggatat gaccttgaat ttggcaaaag
     6121 atttactgaa accgaaggtt atttgaaaaa ctttggtaca ttttttagag tatacaactt
     6181 tgccggtgat gatacgataa ccatgtctgg aattggtgtt aaaattgata aaacctttgg
     6241 agatcctaat gatgtcaatt ataagttagg tgtagattgg cagaatgata acattagggg
     6301 atcaagtact gaggctactt ttgaaatttc aattccttta ggtaagtcat cgaaatcaag
     6361 tatagataaa ccaaatgatg aattggaaaa taggatgact gaaaaagcca gaagggatat
     6421 tgatgtaatt attggagaaa ctaagccaga aagagtttta gaagctaaag aagtagctca
     6481 ggacactgat ggtgacaata catttatacc agccggtaaa gtctggtatg tttcagctaa
     6541 tggtgatgga aatggtagta aagatgatcc tataaatctt aaagatttat tcgctcccgt
     6601 caacgccgaa agtatagaat taaatagagc gggtaataat actaatattg cagaagaagg
     6661 agatattatc atcctactcg gtaatgaagg tgatttttta ctaactccag aagaaataaa
     6721 ttcattaagg ttagaaccca gacaaaaatt aatatcacca gggggatacc taaaaatata
     6781 ctcagaatct gatcagacca gataccagta ttttaaaccc gaaggaaaaa ctgctactat
     6841 caaatctgat aactcaattg aacaagtcaa tacacagtta cgcataggca ttgcagatat
     6901 ggttcagctc acaaataata atacagtctc cggtattaat tttgtggggg ctagcgatga
     6961 agccttattt ggaaatggag aggcagtagt aggagataat gtaactggta aaatcaatat
     7021 tactaacaat actttctcaa acttagaaac agctataaac atcaaaggtt ttaaaggaga
     7081 gtctaaaata aatataaaaa ataatagatt agataatgtt gcctttggta tatcagtagg
     7141 taatgaagac atcttcagag gcggacccat tatgcctgct aataccaatc aatcagttga
     7201 atttacttcc ccgctaattc atatgcaatc tgaaatcggt gatataaaag tcacaatttc
     7261 agaaaatata ataaacgatt ctctttttta tggtatctta accaatttag ataggtctac
     7321 aaaccattct ataacaatag acaataatga gatagattta agagatttga atgaggtaat
     7381 gattgaagaa gatggtttta tctctcctat gcctcctttt atgatgggta atggtatatt
     7441 tgtaaataca gacaatgcta tcaatgctga agttaaaata tataaaaaca agatcaagta
     7501 tgcctatcaa gatggtatct tagttaccag ttggaaccct agggaaacta atgatataca
     7561 gatctataaa aatcaaattg gtaaggttgg cagtccttat attgattatt acgactatga
     7621 agcaaaccta acagatttta gtagtccatc agaatatgat cttgtaaata atggaattac
     7681 agtggcagta ttaaat
//
LOCUS       DATGCJ010000225        12484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_78284, whole genome shotgun sequence.
ACCESSION   DATGCJ010000225 DATGCJ010000000
VERSION     DATGCJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78284"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2101
                     /locus_tag="VKN64_10050"
     CDS             <1..2101
                     /locus_tag="VKN64_10050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA60625.1"
                     /translation="QVKREVKDDPFSFAVLGNIKNSISIFDKGILPRLRSDRPDFIIS
                     TGNNVVDSGEGKYRVLYRTLQRMKIPFITSVGENEIEEEGHKNFYKYFGPFYFSFNLD
                     DSYFIFLDTTGHSSESWQREWLNKELEVSLDYENTFIVMNKPPLKIEAEYLLEDKIKY
                     IESAEKRNYYQNLFAEYNVDSVFSSNLQLYKKESIQGVNYIVSGGAGGELIVESPDSY
                     YHYVRVNVNSGGINYNVEKLEGSPNIFTKALVNVWIAIQSFLFTNYLTLIIIALIVTM
                     AGFLFYWELNREVNYYRDFKYPEQYFEDKKLKIAMFTNNYFPVIGGVPISIDRLARGL
                     RELGHEVYIFAPDYPEHQEDDDKVIRCKLLYHFQKEGMDMVIPNVYNSEIEDKFKAHN
                     FDLVHAHHPFLLGAKGRSLGAKFDKSVVFTYHTRLEKYAHYLPGFFFIRRLFENRVSH
                     LMIKRFANKCEGIFAPTDSTREYLRNIGVRSNIRVLPTGVDLERYEHDEKELVPLKEK
                     YIEKEEITLITVSRLSKEKNLYFLLDGLEQIKERTDKRFKCLIVGDGPEKDNLKEYVK
                     KKEIEGNVEFVGAVDYQEINKYYLIADLFIFASTTETQGMVLLEAMAGYTPVVAVRSS
                     GIDDVVENGFNGYKTAEDISAWSDKVIKLIKDKELYNKTADNARKTAEKYSLKNMAKE
                     AAEMYREVLKIKQSKSE"
     gene            2272..3684
                     /locus_tag="VKN64_10055"
     CDS             2272..3684
                     /locus_tag="VKN64_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMA60626.1"
                     /translation="MKERDQLIAENLNKILDNINEGIHVVNEVGQTIIYNKKMSELEG
                     MDKDIVLGKKIDEVFPSLNEENSTLLLTLRNREQIKNNQQEYLNKYGHKIITINSTIP
                     LYLENGTVWALEIARDITETKKLYNKIVSLQEKLYTDEGFEEEHNKFFTFSDIKGNNK
                     KLKQVINYARQAARTDSSILIVGETGTGKELFAQSIHSGSRRQNKPFIAQNCAALPEN
                     LLEGILFGTRKGGFTGAINRKGLFEQANGGTLLLDEINSMSKALQAKLLRVLQEGLVR
                     PVGGKEDIEVDVRIIATSNKTPVESIREGNLREDLYYRLAVVLLQLPLLSERKDDILL
                     LADHFIDRINRKFNRNIRGLSDEVKEIFYNYKWPGNVRQLEHVIEGAINILSDGDYIQ
                     KEHVEPFLIDINSTNNDKKQMDEKKTLPEKMEKLEKEYIIEALKKNGKNITNTAKYLG
                     IKRQSLQYRLNKYNIELEEL"
     gene            3857..5539
                     /locus_tag="VKN64_10060"
     CDS             3857..5539
                     /locus_tag="VKN64_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMA60627.1"
                     /translation="MNNGIDLINRGKNILELTEELVNIESVSNTDGVIEIGNHIHDML
                     EEWPYFQKNPDHLLKLPLRNDKMNRFNLAALVKGDNSSEKTVVYIGHMDTVDVGEYRE
                     LKDLATKPYELMDKLKKRDLDQDVQEDLNSGEWLFGRGTADMKSGVAVELELLKEFSE
                     NTEDLEGNVLVIITVNEEADSLGMLNIAKPLVELAKKENLQYIGGLNTDYTTDEIGTN
                     PENRYIYMGTLGKIVPALYVVGKGSHVGDVFGGFDVNLLMSELTSRIDNNMELADKYN
                     DRITNPPVSLKQEDQKLEYNGQLPYEGFAYYNFLNFNRGVTEVMETFKEEVKDSIRSS
                     LDWVEGNFRVFKSYHPEQEMNLDLDINCYTYQELLDIVKEKYIEEKVEAEIENVLSRV
                     EEEGIKDLRVHSLRIIKRMWELAELEGPAAIIFLVPPFYPPQNPNVEDEKFKKFNSIA
                     RETINNHGINDDYNYNMIIKDFFPFLSDASYAAFQGDKKDEETLKSNMPYWNKGWRID
                     IEAVRELNLPVVDVGVYGKGAHKYLERVHIPYTMKVLPKLIKTITKKALQNY"
     gene            5654..6733
                     /gene="dctP"
                     /locus_tag="VKN64_10065"
     CDS             5654..6733
                     /gene="dctP"
                     /locus_tag="VKN64_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HMA60628.1"
                     /translation="MLKSKKLIVLSLVLSFLLVFGGLAGAQEYEMVVSHYTITDAEIS
                     GIAASLVYFENMVETRSGGSIDVKVFGNSSLGGVQEVTRQTVKGDTVQSSIAYGGVFA
                     TFFEKYQLMYSPYLFENYRSAWEFMDSDFFAEFMDEARQETGLRHLGIYDDGGGFVAF
                     TNNKKLIKTPEDMEGLRIRTEGGNAAQLAMIEALGAEAVPLPWGDVTTALASGVADGQ
                     FNAPYVNAFAKFWEVNDYTSWIKFIFNTAVWSVSDEWFQTLPEKEQNIILTTAEEAID
                     IARGINAQQSMLDWDEADEHFEDTYNPPPEVMEEWKEVLRPAFYDWVTEDFGIPAEEV
                     DEFWNKAEEIGNEVDSKIISNYGVK"
     gene            6820..7341
                     /locus_tag="VKN64_10070"
     CDS             6820..7341
                     /locus_tag="VKN64_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HMA60629.1"
                     /translation="MKYIQKVSDFLDTLTIRIASFFVALILIIAVYGAFFRYILGSPL
                     PWPLPVERILMIWSALFGIAAAFKRNQHMGVEGFIKAMPDKFERVMRYIIFTLIFLFM
                     GALFWYGLQETINNNDTYMITRSIRISSKWLVAAIPVSAIIHLIHLFTAPNMIEERIK
                     LDLSDEIMEEAGN"
     gene            7364..8653
                     /locus_tag="VKN64_10075"
     CDS             7364..8653
                     /locus_tag="VKN64_10075"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HMA60630.1"
                     /translation="MLIVFVVFFIIIAIGVPIAYAIGLTGMFGLFMAGGTSFFSIMST
                     RLLAAVSDFILISIPLFILTSEFLNRSGMTEKIVNFALSFMGRFRGGLSHVNIGASIL
                     FAGLTGTAVTDTAAIGNILIPAMKKRGYGAAYSAAVTASSSVIGPIIPPSLIMIVYAS
                     MFEEVSVISMFAAGIIPGIIIGLALLVISTVTSMRRNYPKEKHYTWKERWDATKQAIW
                     ALLTPLIILGAILSGITTITEAGALAALYAFLVGVFIYKNLGWKEIYDSLKNSVVLSG
                     VVFFLIGSANILGWVVTYSGLTEVLGTAMINFSESHIIQLLMVNILFLIVGMFLDIGP
                     AVVLLAPIVTPVMVQLGFDPLHFAMIMMVNVNIGNATPPMGMTLMVGSQIARVPYEKA
                     IKEVAPFILAEIGALLLISYIPELTLWIPRILGLSWG"
     gene            8758..9879
                     /gene="ald"
                     /locus_tag="VKN64_10080"
     CDS             8758..9879
                     /gene="ald"
                     /locus_tag="VKN64_10080"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMA60631.1"
                     /translation="MIIGVPKEIKSNENRVAITAAGVKAFVNDGHKVVIEKNAGEGSG
                     ISDEQYVSAGAEMLDTPKEVFAEADMIMKVKEPLEEEYDYFKEGQILFTYLHLAAEEK
                     LTKALMEKNVVSIAYETIQLEDGSLPLLTPMSEVAGRLATQEGARFLEKPQGGKGVLL
                     GGIPGVTPAKVVIIGGGIVGMNSAKMAVGLGADVTILDIDPAQMRYIDDVFNGRVKTL
                     MSNEHNIRYAIQDADLVIGAVLIPGAKAPDLVTEDMIKDMPEGSVLVDVAIDQGGCIE
                     TTYPTTHADPVFTKHGVVHYSVANMPGAVARTSTFGLTNCTLPYALNLANKGYKQAMI
                     DDLALAKGLNVYEGDITYKAVAEAFDMEYTPLEDVIKKF"
     gene            9907..10941
                     /gene="ord"
                     /locus_tag="VKN64_10085"
     CDS             9907..10941
                     /gene="ord"
                     /locus_tag="VKN64_10085"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HMA60632.1"
                     /translation="MENIKVVLWGMGSMGSGMGKYLVDKKGIEIVGAIAHRESKAGKD
                     VSEFFGLDKEVGVKVTNDPMSVITEDVDIVLQATSSFVKDVYPQIEQIVKKKVNVISI
                     AEEMAYPSVQEKELSEKIDKLAKEYGVSVLGTGINPGFVLDLMIIALSGACAKVDKIE
                     AARINDLSPFGPTVMRTQGVGTTVEEFEKGVKDGSIVGHIGFLESIHMIADRLGIDLD
                     KVEQTREPIVSETHRETEHVKVEPGMVAGCRHIARGYSDGEEVILLEHPQQIHPEKEG
                     TETGDYINIKGIPDVNMSIQPEIPGGIGTMAVAINMIPVIVGAEAGLVTMKDLPVPAA
                     MIGDARKLME"
     gene            10970..11278
                     /gene="ortA"
                     /locus_tag="VKN64_10090"
     CDS             10970..11278
                     /gene="ortA"
                     /locus_tag="VKN64_10090"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HMA60633.1"
                     /translation="MGEVIKAGSWVQIYQVVLPAGERAPQVPEDTQKVPLELKVKGFL
                     QEDAELGDEVEIKTVIGRTHKGELIEVNPPYEHKFGKMIPELLQVGPQIRKILRDEEV
                     "
     gene            11282..>12484
                     /gene="ortB"
                     /locus_tag="VKN64_10095"
     CDS             11282..>12484
                     /gene="ortB"
                     /locus_tag="VKN64_10095"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HMA60634.1"
                     /translation="MAANDYNSVMARKNEIMKKAVGIDYERFATGDIGFDYERMMSEV
                     GYKLDEIIKFQRETAVGQTPFIELKNITELARKLAPAGKGARIFLKDEAANPSGSYKA
                     RRASISVYHAEKEGYPGVVAATSGNYGAAVASQAVMRNLESIIVQEVFDSRGVGQPEI
                     LEKGRKCEAYGSEVLQLSVGPELFYTFLRVLEDTGYFNASLYTPFGIAGVEPLGYEIV
                     QELQEREGKNPDAVIITHAGGGNLTGTSRGLIKAGADETKIVAASVDLSGLHMASDHD
                     FNRKSFTTGHTGFGVPFATWPDRADVPRNAARALRYMDEYVTVKQGEVFYITEALAQI
                     EGMERGPAGNTSLAAAFDLAQKMDDDEIIVVQETEYTGAGKHPMAQLNFAKENGIDVR
                     NGDPDEEEA"
BASE COUNT         4183 a         1976 c         2756 g         3569 t
ORIGIN      
        1 ccaggtgaaa agggaagtaa aagatgatcc tttttccttt gcagtgttgg gcaatatcaa
       61 gaattcaatc agcatttttg ataaaggcat tttacctaga ttacggtctg ataggcctga
      121 tttcattatt tccaccggta ataatgttgt ggacagcgga gaaggtaaat atagagtttt
      181 atataggacc ttacaaagaa tgaaaatccc ttttatcacc agtgtgggag aaaatgagat
      241 cgaggaggaa ggccataaga acttctataa atacttcgga cccttctatt tttcttttaa
      301 tctcgatgat tcctacttta tctttctcga tacgaccggc cacagttctg aaagctggca
      361 gcgggaatgg cttaataagg aattagaagt ttctttggat tatgaaaaca cttttatagt
      421 aatgaataag cctcctttaa agatagaggc ggagtacctt ttagaggata agatcaaata
      481 tattgaaagt gccgaaaaaa gaaattatta tcaaaactta ttcgcagaat ataatgtaga
      541 ttcggttttc agttcaaatt tacagttata taagaaagaa agtattcagg gcgtaaatta
      601 tatagtaagt ggaggagctg gtggtgaact tattgttgaa agcccggata gttattatca
      661 ttatgtaagg gttaatgtaa attcaggtgg tattaattat aatgtagaaa aattagaggg
      721 cagccctaat atctttacaa aagccttagt aaatgtctgg atagccatcc agtccttctt
      781 atttaccaac tacctcactt taatcatcat tgccctgatt gtaacgatgg ctggattttt
      841 attctactgg gaattaaata gagaagtcaa ctactaccgc gactttaaat atccagagca
      901 gtattttgaa gataagaaat taaagatagc catgtttacc aataactact ttcccgtgat
      961 tgggggagtg cccatctcga tagatagatt agcccgcggt ttaagagaat taggacatga
     1021 ggtctatata tttgcacctg attatcccga gcaccaggaa gatgatgata aggtaataag
     1081 gtgtaaattg ctttaccact tccaaaaaga gggaatggat atggtcattc ccaatgtata
     1141 taacagtgag atagaagata agtttaaagc ccataatttt gatctcgtac acgctcatca
     1201 tccctttcta ttgggagcga aaggaaggtc cttgggagct aaattcgata aatcggtcgt
     1261 ttttacatac cataccaggt tagaaaagta tgctcattat ttaccgggct tcttttttat
     1321 ccgcaggcta tttgaaaaca gggtttctca tttaatgata aaaaggtttg ctaataaatg
     1381 tgaagggatc tttgccccga ccgactctac ccgggaatat cttcgtaata taggggtccg
     1441 tagtaatatc agggtattac cgaccggggt tgatcttgaa aggtatgaac atgatgaaaa
     1501 ggaattagtt cctttaaaag aaaaatatat tgaaaaagaa gagataacct taataactgt
     1561 ttcaagatta tccaaagaga aaaacctcta tttcctattg gatgggcttg agcagatcaa
     1621 agaaagaacc gataagagat ttaaatgttt gatcgtggga gatggacctg aaaaagacaa
     1681 tttaaaagaa tatgtaaaga aaaaagagat agaaggaaat gttgagttcg tgggagctgt
     1741 tgattatcaa gagataaaca agtactatct gatagcagat ctctttatct ttgcctctac
     1801 taccgagacc cagggcatgg tcctcttaga ggctatggcc ggctatacac cggtagtagc
     1861 agttaggtca agcggtattg acgatgttgt agaaaatggt tttaacggtt ataaaacagc
     1921 cgaagatatt tcagcctggt cagataaagt tattaaattg ataaaggata aagaacttta
     1981 taataagaca gccgacaatg cccgcaaaac agctgagaag tattccctta aaaatatggc
     2041 taaagaagca gccgagatgt atagggaagt attaaaaata aaacagagta aaagcgaatg
     2101 agtttctaaa ataaatctaa aagagaacct ccctttttcc ttcctgctag ggaggtttag
     2161 cttgctttaa aaaataatca gtttagtgaa tttttaaaca atataataat aaattcagga
     2221 ggagatgtcc tttagataga gaattaataa acagggaagg aggttttttg attgaaggaa
     2281 agagaccaat taatagctga aaatttaaat aaaatactgg ataatataaa tgaaggaata
     2341 catgttgtta atgaagtggg tcagactatt atttataata aaaaaatgtc ggaattagaa
     2401 ggaatggaca aagatatagt actgggcaaa aaaatagatg aggtttttcc ttctctaaat
     2461 gaagaaaaca gtacactgct gttaacctta agaaatagag agcaaataaa aaataaccag
     2521 caggaatatc taaataagta tgggcataaa atcataacga ttaatagtac gatcccgctt
     2581 tatttagaaa atggtacggt ctgggcctta gaaattgccc gggatattac cgaaaccaag
     2641 aaactttata ataagatcgt atctctgcag gaaaaactat atacagatga aggttttgaa
     2701 gaagaacata ataagttctt tacctttagt gatataaaag gtaacaataa aaagcttaaa
     2761 caggtaataa attacgcccg acaggcggcc cggacagatt cttctatctt aatagtaggt
     2821 gaaaccggga ccggtaaaga actttttgcc cagagcattc acagcggcag ccgccgtcag
     2881 aacaaaccat ttattgccca aaactgtgcc gccttaccgg aaaacctcct ggaaggaatt
     2941 ctctttggaa ccaggaaagg tggttttaca ggggcaatta accggaaagg actatttgaa
     3001 caggccaatg ggggcacact tcttttagat gagatcaact cgatgtcaaa ggccttacag
     3061 gccaaactat taagggttct ccaggaaggt ttagttcgac cggtgggggg caaagaagat
     3121 attgaggtag atgtaagaat tattgcaacc agcaataaga ctccggtaga atcgatcagg
     3181 gaaggtaatt taagggaaga tctatactac cggctggcag tagttttatt acagttaccc
     3241 ttactctcgg aaagaaaaga tgatatttta cttttggcgg atcattttat agatagaata
     3301 aaccgtaaat ttaatagaaa tatcaggggt ttaagtgatg aagtcaagga aattttctat
     3361 aactacaaat ggccgggtaa tgtaaggcag ttagaacatg taatagaagg tgctataaat
     3421 atattaagtg atggagacta tattcaaaaa gaacatgttg aaccattttt aattgatatt
     3481 aatagtacca ataatgataa aaagcagatg gacgagaaaa agaccctgcc tgagaagatg
     3541 gagaaattag aaaaggaata tattatagaa gctttaaaga aaaatggtaa aaatattacc
     3601 aataccgcaa aatatcttgg cattaaaaga cagagtttac agtatcggtt gaataaatat
     3661 aatattgaac tagaggaact ttaattatta ataaatgtat aaactttata tttgtggtgc
     3721 tgatagccgg ttgttatcag tatcacaatt tttttatgtc aaaagtgact ttctggcacg
     3781 acatatgcat aaatatatag gtggaacata aatatgatag ttttatgcaa aatattttct
     3841 taggggtgtg gtcttattga ataacggtat tgatctgatt aatagaggca aaaatatttt
     3901 agaacttacc gaagaactgg ttaatattga aagtgtcagc aatactgatg gagtaattga
     3961 aattggtaac catattcacg acatgctgga agaatggcca tattttcaaa aaaaccctga
     4021 tcatctatta aaactaccgt taagaaatga taagatgaat agatttaatc tggcagcttt
     4081 agtaaaggga gataattctt ctgagaaaac ggttgtatat atcggacata tggatacggt
     4141 agacgtaggt gaatataggg agttaaaaga tctggctaca aaaccttatg aactgatgga
     4201 caagttaaag aaaagagatt tggaccaaga cgtgcaagaa gatttaaact caggtgaatg
     4261 gttatttgga cgtggaacag ctgatatgaa atccggcgtt gcagttgagc tggaactgct
     4321 caaagaattt tctgaaaata ctgaagattt agagggtaat gtgttagtaa ttattactgt
     4381 taatgaagaa gcagattcac tcggcatgtt aaatattgcc aaaccattag tcgaattagc
     4441 aaaaaaagaa aacttgcaat atattggtgg ccttaatacg gactatacta ccgatgaaat
     4501 tggtactaat cctgaaaata ggtatatcta tatgggtact ctgggcaaga tagtgccggc
     4561 cctctatgtg gtcgggaaag gctcacatgt aggtgatgta tttggcggct ttgatgttaa
     4621 tttattgatg tcagagttaa cttctagaat tgataataat atggagttgg ctgataagta
     4681 taatgatcgg ataaccaacc ccccggtttc cttaaaacag gaagatcaga aattagaata
     4741 taacggccaa ctcccctatg aaggttttgc ctattataat ttccttaact tcaatcgggg
     4801 agtaaccgaa gtgatggaga cttttaaaga agaggttaag gatagtataa gatcttccct
     4861 ggactgggtg gaaggtaact ttcgggtctt taaaagttac caccccgaac aggagatgaa
     4921 tctggattta gatatcaact gttataccta tcaggaactt ttagatattg taaaagaaaa
     4981 atatattgaa gaaaaagtag aagcagagat tgaaaatgtc ctatcaaggg tagaggaaga
     5041 agggattaaa gaccttagag ttcattctct tagaattata aaaagaatgt gggagttggc
     5101 cgagttagaa ggcccggccg ccattatctt tttggtacca ccattttatc ctccccaaaa
     5161 tcccaatgtt gaggatgaga agtttaaaaa atttaattct attgcccgtg agacaattaa
     5221 taaccacggt attaatgatg actataatta caatatgatt ataaaagact ttttcccgtt
     5281 tttatctgat gctagttatg cggcctttca gggtgataaa aaagacgaag aaaccttaaa
     5341 aagtaatatg ccgtactgga ataaaggctg gcgaatcgat attgaagccg taagagagct
     5401 aaatttaccg gtagtagatg taggtgtata tggaaaaggt gcccacaaat atttagaaag
     5461 agttcatata ccctatacta tgaaagttct accgaaacta ataaagacca taaccaagaa
     5521 agcgctgcaa aattattaat caagtgaaaa caaattttgc ggtgagcagg gggtgattat
     5581 gggaaaggag acctaattag agaagctgag tagagaaatt aggcaaattt aaaaaagggg
     5641 gaaataataa ataatgttga aaagtaaaaa gttaatcgtt ctatccttag ttctgtcttt
     5701 cttattagta tttggcggcc tagcaggtgc ccaggaatat gaaatggtag tttcccacta
     5761 tacaattacc gatgctgaaa ttagtggaat cgccgcttcc ttagtatact ttgaaaacat
     5821 ggtagaaacc agatccggcg gttctattga tgttaaagta tttggcaaca gttccttagg
     5881 cggtgtccag gaagtgacaa gacagaccgt taagggagat acagttcagt cctcaatcgc
     5941 atatggtggt gtttttgcaa ctttctttga aaaataccaa ctaatgtatt ctccttatct
     6001 atttgaaaat taccgttccg cttgggaatt catggacagt gacttcttcg ctgaatttat
     6061 ggatgaagca agacaggaaa caggcttaag acacttgggt atctacgatg atggcggcgg
     6121 ttttgttgcc tttactaata acaaaaagtt aataaagaca cctgaagaca tggaaggttt
     6181 aagaatcaga actgaaggcg gaaacgcagc tcagttagct atgattgaag cattgggcgc
     6241 agaagcagtt ccacttccct ggggagatgt aacaactgct ctagctagtg gtgtagctga
     6301 tggtcagttt aatgctcctt atgtaaatgc atttgctaag ttctgggaag taaatgacta
     6361 tacatcctgg attaagttca tcttcaatac tgctgtctgg tcagttagtg acgaatggtt
     6421 ccaaactcta ccagaaaaag aacaaaatat tattctaaca actgcagaag aagctattga
     6481 cattgcacgc ggtattaatg cccaacaatc aatgttagac tgggatgaag cagatgaaca
     6541 ctttgaagac acctataatc ctccaccaga agttatggaa gaatggaaag aagtacttag
     6601 acctgccttc tatgactggg taacagaaga ctttggcatt ccagctgaag aagtagacga
     6661 attctggaat aaagctgaag aaataggtaa tgaagtagat agtaaaataa tttctaacta
     6721 tggagtaaaa taaatagcag ctattaatga caacaattaa tagaagggga tcccctttaa
     6781 ggggattccc tactaacata tagagaaggg gtgaaagttt tgaagtatat tcagaaggtt
     6841 agcgattttt tagatacact tacgattagg atagcatcct tttttgtagc attaatctta
     6901 attatagcag tatacggggc attttttcgt tatattttag gcagtcctct accctggcct
     6961 ctcccggttg aaaggatttt aatgatctgg agtgctttat ttggaatagc agctgccttt
     7021 aaaagaaacc agcacatggg tgttgaggga tttattaaag caatgcccga taaatttgaa
     7081 agggtaatga gatatataat ttttacctta atatttttat ttatgggagc cttattttgg
     7141 tacggactac aggaaacaat taataataat gatacctata tgattacaag aagtattaga
     7201 atcagttcga aatggctggt agcagcgatt ccagttagcg ctataataca tttaatacat
     7261 cttttcacag caccgaatat gatagaagaa agaataaaac ttgatctttc cgatgaaatc
     7321 atggaagagg caggaaatta aatatgaaag gagataaaaa gctatgctaa ttgtatttgt
     7381 tgtatttttc attattatag caattggtgt gccgatagca tatgctatag gtttaaccgg
     7441 tatgttcggt ttatttatgg ctggtggaac cagctttttt agtatcatgt ctacccggct
     7501 tttggccgca gtttcagact ttattttgat ctcaattccg ctgtttatct taacttcgga
     7561 gttcttaaat agaagtggta tgactgaaaa aattgtaaat tttgcactta gctttatggg
     7621 gagattcaga ggtggacttt cccatgttaa tatcggggcc agtatcctct ttgcagggct
     7681 taccgggact gctgtaaccg atacagccgc gatcggtaat atcttgattc ccgccatgaa
     7741 aaagagaggt tatggagcag cttatagtgc agctgttaca gcttcatctt ctgttattgg
     7801 accgattatt ccacccagtt tgattatgat cgtttatgcc agtatgtttg aagaagtttc
     7861 agtaatttcc atgtttgcag ccggaattat acctggtatt attattggtc tagcactctt
     7921 agttattagt actgtaactt cgatgagaag gaactatcca aaagaaaaac attatacctg
     7981 gaaagaaaga tgggatgcga ccaaacaggc catctgggcg cttttaacac cgctcatcat
     8041 cttaggagct attctatcag ggatcacaac cattacagag gccggtgctt tggccgcctt
     8101 atatgcattt ttagttggag tatttatcta taagaattta ggctggaaag aaatttacga
     8161 ttctcttaaa aacagtgttg ttttatcggg agttgtcttt tttctaatcg gttcagctaa
     8221 tattttaggc tgggttgtta cttacagcgg tttaactgaa gtcttgggaa cagctatgat
     8281 caatttcagt gaaagccata ttatccagct tttaatggtt aacattctct ttttgatagt
     8341 tggtatgttt ttagatatcg gaccggcagt tgtactttta gcaccgattg taactcctgt
     8401 tatggttcag ctcggttttg accccctcca ctttgcgatg attatgatgg ttaatgttaa
     8461 tattggtaat gcgactcccc cgatgggcat gacgcttatg gtcggctctc agattgcgag
     8521 ggtcccgtat gaaaaagcca ttaaagaagt ggccccattt atcctggctg aaataggtgc
     8581 ccttctctta atttcataca ttcctgaatt aactttatgg attcctagaa ttttaggttt
     8641 aagttggggc taaagtatag taaaatagta gatgctggca taattattgc atatatattt
     8701 atatgtgaat aaaatgttaa aattatcaag ttaaaaaatt aaaaggagtt gatttatgtg
     8761 attattggtg tacccaaaga aattaagtct aatgaaaatc gtgttgcaat cacagcagca
     8821 ggagtaaagg catttgttaa cgatgggcac aaagttgtaa ttgagaaaaa tgcaggtgaa
     8881 ggcagcggta ttagtgatga acagtatgtc agcgcgggag cagaaatgct agatacccca
     8941 aaagaagtat tcgctgaagc tgatatgatt atgaaggtta aagaaccgct tgaagaagag
     9001 tatgattact ttaaagaagg tcaaattctc tttacatatc tgcacttagc agcagaagag
     9061 aagttgacta aagctctcat ggaaaaaaat gttgtttcta tcgcttatga aaccattcaa
     9121 ttagaagatg gttccttacc acttcttaca ccgatgagtg aagttgccgg ccgtttagct
     9181 actcaagaag gcgccagatt tttagaaaaa ccacaaggcg gtaaaggagt acttttaggc
     9241 ggtattccgg gcgtaacccc agctaaagta gttattatcg ggggcggaat cgttggtatg
     9301 aactcagcta agatggctgt aggcttaggc gcagatgtaa caatcttaga tatcgatcca
     9361 gcccaaatga gatatattga tgatgtattt aacggccggg ttaagacttt aatgtccaat
     9421 gagcataata ttagatatgc tattcaggat gcagacctag taatcggggc tgttttaatc
     9481 ccgggcgcta aagcacctga cttagtaact gaagatatga tcaaagatat gccagagggc
     9541 tcagtattag tcgatgttgc aattgaccag ggcggctgta ttgaaactac ttatccaact
     9601 actcatgcag atcccgtctt taccaaacac ggagtagttc attattcagt tgctaatatg
     9661 ccgggtgcgg ttgctagaac ctctaccttt ggtttaacta actgtacatt accatatgcc
     9721 ttgaacttgg ctaacaaagg ctacaaacag gctatgatag atgatcttgc tcttgccaaa
     9781 ggtcttaatg tatatgaagg cgatataaca tataaagcag tggctgaagc atttgatatg
     9841 gaatatacac cgctggaaga tgtaatcaag aaattctaaa aataatttac caggaggaga
     9901 tttaaaatgg aaaatattaa ggttgtccta tggggcatgg gctctatggg aagtggaatg
     9961 ggtaaatacc tagttgacaa aaaaggaatc gaaattgtag gcgctatcgc ccacagagag
    10021 tcaaaagccg gtaaagatgt aagtgagttt tttggcttag ataaagaagt aggggttaaa
    10081 gtaactaatg acccgatgag tgtaattact gaagatgtag atatagtact tcaggctact
    10141 tcctcttttg ttaaagatgt ttatcctcaa attgaacaaa ttgttaaaaa gaaagttaac
    10201 gttatctcta ttgcagaaga gatggcttat ccctcagttc aggaaaaaga gctaagtgag
    10261 aagatagata aattagcgaa agaatatggt gtttctgttc ttggtacagg tattaacccc
    10321 ggctttgttt tagacttaat gattattgcc ttaagtggag cctgtgcaaa agttgacaag
    10381 atcgaagccg ctagaattaa tgacctttca ccctttggac cgaccgttat gagaacccag
    10441 ggtgtaggta caaccgtaga agagtttgaa aaaggagtta aagacggttc tatcgtaggc
    10501 catattggtt tcttagaatc aatccatatg attgctgacc gtttaggaat cgacttagat
    10561 aaagttgaac agaccagaga acctatcgtt tctgaaacac atcgagaaac cgaacatgtt
    10621 aaagtagaac ccggtatggt agccggttgt agacatattg ccagaggtta cagcgatggt
    10681 gaagaagtaa tcttattaga acacccacag cagatccatc ctgaaaaaga aggtacagaa
    10741 accggtgact atattaacat caaaggtata ccagatgtta acatgtcaat tcaaccagaa
    10801 attcccggtg gaatcggtac aatggcagta gcaattaaca tgatcccagt tatcgtagga
    10861 gctgaagcag gcttagttac catgaaagat ctaccagtac ctgctgcaat gatcggtgat
    10921 gcacgcaaat taatggaata aaaacaatat aaattttgga ggtatttata tgggtgaagt
    10981 tataaaagct ggaagttggg tacaaatcta tcaagttgtt cttcctgctg gagagagagc
    11041 tccccaggta cctgaagata cacagaaagt tcctttagaa ttaaaggtaa aagggttctt
    11101 acaagaagac gcagaacttg gtgatgaagt agaaatcaaa acagtaatcg gtagaactca
    11161 taagggagaa ttaattgagg tcaacccacc ctatgaacat aaatttggga agatgattcc
    11221 cgaactttta caggtggggc ctcaaattag aaaaatatta agagacgagg aggtgtagag
    11281 attggcagca aatgactata attcagtaat ggccagaaaa aatgagatta tgaaaaaagc
    11341 ggttggtatt gattatgaaa gatttgcaac cggtgatatt ggctttgact atgaaagaat
    11401 gatgtcagaa gtcggctaca aattagatga aataattaag tttcaaagag aaaccgcagt
    11461 cgggcagacc ccatttattg aactaaaaaa tattacagag ttagccagaa agttagctcc
    11521 agctggtaaa ggagccagga tatttctaaa agacgaagct gctaatccct ctggtagtta
    11581 taaagccagg cgagcctcta tctctgtata tcatgctgaa aaggaaggtt accccggtgt
    11641 agttgctgca accagtggta actatggggc agctgttgct tctcaggctg taatgcgcaa
    11701 cctggaatca ataatagtac aggaggtttt tgacagtcgg ggtgtcggac aaccggaaat
    11761 ccttgaaaag gggcggaaat gtgaagctta cggttcggaa gtcctgcagt taagtgtagg
    11821 gccagaactt ttctatactt ttcttagagt attagaagat accggctatt tcaatgcctc
    11881 tctttataca ccctttggga ttgcaggtgt tgaaccatta ggttatgaga tagtccagga
    11941 attacaggaa agagaaggta aaaaccccga tgcagttatc ataactcatg ccggcggcgg
    12001 taacttaaca ggtacttccc gcggtttaat caaggcagga gccgatgaaa ctaagatcgt
    12061 ggcggccagt gttgacttaa gcggtctgca catggcaagc gaccatgatt ttaatagaaa
    12121 atcatttaca accggacata ccggttttgg agttcctttt gcaacctggc cggatagagc
    12181 ggatgttcca cgcaatgcgg cccgtgcttt aagatatatg gatgaatacg taaccgttaa
    12241 acagggagaa gtcttctata tcactgaagc actagctcaa attgaaggaa tggaaagagg
    12301 gccagctgga aatacttccc tggcagctgc ctttgattta gctcagaaga tggatgatga
    12361 tgaaataatt gtagtacagg agactgaata taccggagcc ggtaaacatc ctatggccca
    12421 gcttaacttt gctaaggaaa atggaattga cgttagaaac ggagacccgg atgaggaaga
    12481 ggcc
//
LOCUS       DATGCJ010000226         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_571152, whole genome shotgun sequence.
ACCESSION   DATGCJ010000226 DATGCJ010000000
VERSION     DATGCJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571152"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VKN64_10100"
     CDS             complement(<1..648)
                     /locus_tag="VKN64_10100"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HMA60635.1"
                     /translation="MIKNNFSLENKLSKKDLELIHNNTVKLLENEGVIVDNKKALKIF
                     EKNGIKTEGKKVYISGQKLEEIVAKIPGNFELKARNPNNNIEVGNDTSILGPTLGPPF
                     VYEDGEKRYGTYDDYIKTIKLCQANPYIDLVGGDIIAPTDLDENNRHKKMFYAAIKYS
                     DKALLGTGSGEQEIKDTLNMGKILFGEDELKNNNYIMKLIGASSPLEYNETALNTL"
     gene            839..1693
                     /locus_tag="VKN64_10105"
     CDS             839..1693
                     /locus_tag="VKN64_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMA60636.1"
                     /translation="MKTLKNKIALVTGASRGIGKGIAKGLAEKGATVYITGRTTDEDN
                     KDAKLSGTINQTAKKVNELGGIGIPYKCDHRNDEEVEALFKKIEKEQGKLDILVNNVW
                     AGYENIYNVKKPEDYIFENNFWELPNKIWDDMFDIGLRSHFVASKLAVPLMLKNKQGL
                     IVNISFYAGQTYISNVPYGVVKSAVDRLSVDMAYELKEYGITSISLYPGLVRTEGVMR
                     NKDFIDLSNSGSPLFIGRAVAALAEDKKVIEKSGQVLIAAELAEEYNFTDIDGTQPRS
                     LRDIYLDT"
     gene            1817..2422
                     /locus_tag="VKN64_10110"
     CDS             1817..2422
                     /locus_tag="VKN64_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60637.1"
                     /translation="MKYTVVLTLVISLVLVGFGGFLVEAQGVNNEVRPGNIEQINPKR
                     ITYQQIYKGNLDLIEIDKQQSAEIGILLNEYFNKRNQLISNIDYNRDELRKTILTNGA
                     LETESVFNEKIVELNEKLMNLRNEYLGKMRAVLTEEQVNKISESNYIVNISNSAINNY
                     QTARSVNRYPIQGMANNNRFSSNTDITSGLQCGINYLQRGY"
BASE COUNT          892 a          359 c          408 g          847 t
ORIGIN      
        1 tagggtatta agagcagttt cattatattc aagaggacta gaggccccta ttaatttcat
       61 aatatagtta ttatttttta attcatcttc tccgaataat atttttccca tattaagtgt
      121 atcttttatt tcctgttcac ccgaaccagt acctaagaga gctttatcag aatatttaat
      181 agctgcataa aacatctttt tatgcctgtt gttctcatca agatcagttg gtgctataat
      241 atctcccccg accagatcta tataaggatt ggcctggcat aacttaattg tttttatata
      301 atcatcataa gttccatatc tcttttcccc atcttcataa acaaaagggg gtcctagagt
      361 agggcccagg atagaagtgt cattaccaac ttcaatatta ttatttggat ttcttgcctt
      421 taattcaaaa ttgcccggaa ttttagcaac tatctcttct aatttttgcc cgctgatata
      481 aactttttta ccttcggttt ttattccatt cttttcaaat atttttaatg cttttttatt
      541 atctactata accccttcgt tttctaatag ttttaccgta ttattatgta ttaattctaa
      601 atccttttta gataatttat tttctagtga aaaattattt tttatcattt aattaattgc
      661 ctccctgcat atttattggt ttatattgtc tcttattcta tcttcgcaat aagtatatca
      721 atataagata gataatcaaa tttatataca aattatttaa aatttacttg atttatctcc
      781 agcattttat atactttaag ggtgtattaa aaattatcac atataaggaa gtgtttattt
      841 gaagacgcta aaaaataaaa tagcattagt tactggggct agccggggca taggaaaagg
      901 tattgcaaag ggactagcag aaaaaggtgc tactgtctat ataacaggta gaaccacgga
      961 tgaagataat aaagatgcaa aattaagtgg taccatcaat caaacagcta aaaaagtaaa
     1021 tgaactgggc ggtataggta ttccctataa gtgtgatcac agaaatgatg aagaagtaga
     1081 agctttgttt aaaaagatag aaaaagagca gggtaaatta gatattttag taaataatgt
     1141 ctgggctggt tatgaaaaca tatataatgt taaaaaacct gaagactata tatttgaaaa
     1201 taatttttgg gagcttccca ataaaatatg ggatgatatg tttgacatcg gtttaagatc
     1261 tcattttgta gccagcaaac tggcagttcc tttaatgtta aaaaacaagc aaggtcttat
     1321 agtaaatatc tctttttacg caggccaaac atatatttca aatgttcctt atggagtcgt
     1381 taaatctgca gtggatagat tgagtgtaga catggcgtac gaactaaaag aatatggaat
     1441 aacctctatc tctctatatc ccggtttagt tcgaaccgaa ggtgtgatga gaaacaaaga
     1501 ttttattgat ttatcaaatt caggatctcc tctatttatc ggtcgagcag tagctgcttt
     1561 agccgaagat aaaaaagtaa tcgaaaagag cggccaggta ttaattgcag cagaattagc
     1621 tgaagaatat aattttacag atatagatgg aactcagcct agatctctta gagatattta
     1681 tttagataca taatgttttg aggttaatag atattataat acttaaaatt gtaaaacatt
     1741 aaactgttaa gcccccatat atttgttact ttcatattgt aaagaaataa aaagccaaat
     1801 ttaaggggcg ataattatga aatatactgt agttttgact ctggttattt ctttagtcct
     1861 ggttggtttt ggaggttttc ttgttgaggc acagggagtt aataatgaag ttcgtcccgg
     1921 caatatagaa caaattaatc ctaaaagaat aacttatcag caaatatata aaggtaatct
     1981 ggatttgatt gaaatagata aacaacaatc agctgaaatc ggaatacttt taaatgaata
     2041 tttcaataaa agaaatcaac ttatcagtaa tattgattac aatagagatg agttgagaaa
     2101 aactatttta accaatggag ctcttgaaac agaaagtgtc tttaacgaaa aaattgtaga
     2161 actcaatgaa aaattaatga atttaagaaa tgaatacctt ggtaaaatga gagctgtatt
     2221 aacagaggag caggtaaata agatatcaga aagcaattat atagttaata ttagcaactc
     2281 agcaattaac aattatcaaa ctgcccgatc agtaaataga taccccattc aaggtatggc
     2341 aaataataac cgattttcat ccaatacaga tattacttcc ggtctccaat gtggaattaa
     2401 ctacctgcaa agagggtatt aaaaaaatat aagatgatag ttataacccc ccttatgaaa
     2461 ttaagggggg ttaaatttat gtcttataat tcaaaaagtt ttgaaa
//
LOCUS       DATGCJ010000227        15212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_78851, whole genome shotgun sequence.
ACCESSION   DATGCJ010000227 DATGCJ010000000
VERSION     DATGCJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15212
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78851"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1166)
                     /locus_tag="VKN64_10115"
     CDS             complement(222..1166)
                     /locus_tag="VKN64_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMA60638.1"
                     /translation="MSSSAIIHTDTLLNKYKNKNYNFNNLNKDFHIDLPRLKKAEVKL
                     IVFAIYVEENYKTNRDGLKKTIQMVDDFYNILENCEELVLAKHYNDILKINNEDKIAA
                     ILAVEGAKSLYSLSALRVFSRLGIKLITLTWNHRNQIGDGVGVNSKCGLTSFGKDFVK
                     EMHSLNMIVDVSHLNEKGFWDVMEISEDPIIASHSNVKRLCDHPRNLTDKQIKAIAKA
                     NGVIGINFYPLFLNTQEKADINDVFEHIDYIKKLVGIDYVGLGTDFDGISDTPKGLAD
                     IRKLGNLKNILFENGYNEQEVEKIFYKNAYRVFKNVLK"
     gene            complement(1248..2405)
                     /locus_tag="VKN64_10120"
     CDS             complement(1248..2405)
                     /locus_tag="VKN64_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMA60639.1"
                     /translation="MANKDTILNNLEEIKDDLINLNDRIGKNPELGFKEFKAVKWLTD
                     YLASHNFKIKKPIGGLETAFKAEYDFNEPGPVIAYLCEYDALPNIGHGCGHNMIGVMS
                     VGAAVALSKYNDLKGTIQVIGCPGEEVGGGKVTLVEKGIFDEVDAAMMVHPSNENRTY
                     STSLAIEAIKFTFHGQTAHAAAAPEKGINALDGVIQLFNGINAIREHLKDDVRIHGII
                     SKGGESPNVVPDLAEAKFYFRARQKDYLKEVIEKGKNIARGAAQMTGCEVEFSNFENS
                     NDNLNPSGKLAATYEKNLKLAGVNNIADPEESLGSTDMGNVSQVVPSIHPYIKIGDEL
                     IGHTVEFRDATMSKKGQQALIKAAQALALTGYDILNDNELLEEIVEEFQSL"
     gene            complement(2428..4878)
                     /locus_tag="VKN64_10125"
     CDS             complement(2428..4878)
                     /locus_tag="VKN64_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMA60640.1"
                     /translation="MSIIDLMIMTIIFLFFIIIVYYFNKKEYRKYSDINTIFFIGMGI
                     LFFISIALSFLSIKQEYLIYTYIMSTTAALLVNYSLNKYLLNKSNKFWPLLHLIYLFS
                     ILVLLQYNNWGNYIEVLSKLLIVTYFLDIGYLVTKSKKINNLHKMFFSTTFILWSIYE
                     FLAIFFSNDLLYLLGLIFPIAIAYEFMNIQLELINEEIKRYGERFKSIFDNASDAFFV
                     LAKDDLRLMNLNKSAREIFNIDSDHLDKIKLEDILTEDSYKKLSDLIKKGKKSNGYPI
                     ELRAESDKGELKYLATDINFMDFNEKNAVIIAMKDKTEQIKLKNKVMENKNKIEKLHD
                     VAIKMENTAKREEIYKQTVEAAEEILDYDVISLDIVKDNKLMAVGKSQNLTDDDVISM
                     SLDEMSLATKAYHEQRTIICNNIPAERDASPVKTQYKSALTVPIQQYGTFQIISEEYD
                     YFTNQDKNLVELLISHSGAALKRLNREEEIRYFGFHDYLTGLYNRDYIEEEIKRLNNS
                     RQLPVSVIIADVNGLKLINDTFGHDDGDKLLKCIAATLDDACRKEDILGRWGGDEFLI
                     LLPGTTKKNILSIIDRIKDRLEQKTYKDIPLSVSFGFATKVEKETDIVDIIDKADRRM
                     YNNKTKNYKQNSEQMLEVLYKKLNKMGYETESHCQRVKSLAEKFARQLKFDQEKIKLI
                     KKTARLHDIGMINIPRELINQESDLSNEEYKIIQTHPEVGFRIANSLNEVNEIAQAIL
                     HHHEWWNGTGYPEQKAGDNIPLYARIISIVDAFDVMTHDQVYKEKISKERAIDRLRSM
                     AGIQFDPKLTKMFIEEVI"
     gene            4976..6274
                     /locus_tag="VKN64_10130"
     CDS             4976..6274
                     /locus_tag="VKN64_10130"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HMA60641.1"
                     /translation="MQTFQFENLYNSAKGYVLNFLDKYLLSYYNLAQIIFLLLLFGIS
                     NILSKKYKERVINLIPDTFVIKETLGDLFKLIIYVILLWLYIIINSILNFPTYTVIIV
                     ANLLTAWIIINLLSDLIKNELLSKIFSILIWTIAALHVLGIYEEIVNILSKISFRSGN
                     IHISLLMVIKGILTLFIFLWIAMKLSSYSEKQIKKLSPLTPSIRVLLTKLLKFVIFTA
                     VILITMSSLGIDLTALAVVGGAVGVGVGFGLQKIVSNFISGIIILLDKSVKPGDVIEI
                     DNVYGQIKSIGLRYISVLTLDGKEYIIPNEDLITKKVINWSYSNNLVRTSVSVGVSYN
                     SDVEKAMELLQEAIAGIDRILSKPPPKIFLNEFSDSSVNIEVKFWIRDPENGINNIKS
                     EVNKSIWKLFKEHDIEIPFPQQDIHFKSLSPQVKEFFQKE"
     gene            complement(6392..7378)
                     /locus_tag="VKN64_10135"
     CDS             complement(6392..7378)
                     /locus_tag="VKN64_10135"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HMA60642.1"
                     /translation="MAEEQEKLLEIKNLKKYFPVKEGVFRKTVAHVKAVDDISFYIKK
                     GETLGLVGESGCGKSTAGRTILRLLEATEGEIWFEGKNLLSLDEKELREMRKEMQIIF
                     QDPYASLNPRMTVADIVGEPIDIHNLASSKKERNEKVADILDTVGLGPEYMKRYPHEF
                     SGGQRQRIGVARALAVDPSLIIADEPVSALDVSIQAQVINLLQDLQKDFDLTYLFIAH
                     DLSVVKHISDRVAVMYLGKLVEVASKKELFDDPKHPYTQSLLSAIPVADPTYETDRII
                     LEGDVPSPVDPPSGCRFHPRCPYAMDVCSEKEPEFKDYGDEHFAACHLLEQE"
     gene            complement(7394..8401)
                     /locus_tag="VKN64_10140"
     CDS             complement(7394..8401)
                     /locus_tag="VKN64_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60643.1"
                     /translation="MSKEKLIDVNNLKTYFHTEEGTVKAVDGVSFEIYPGETLGIVGE
                     SGCGKSVTSLSIMRLIESPPGKIEEGEILFDGKDLTELNQTQMRKIRGNDISMIFQEP
                     MTSLNPVYTVGDQIMEALLIHKDINKKQAREEAINMLRKVGIPLPEQRVDEYPHQLSG
                     GMRQRVMIAMALSCDPQLLIADEPTTALDVTIQAQILELMNSLKDQFDMAIMMITHDL
                     GVIAEVSDRVAVMYAGKIVEYADVETLYADPKHPYAWGLMHSIPHIEKDLERLEAIPG
                     NVPNPLNFPDGCKYNTRCPFATDKCRTEEPELEEVEEGHYSACWHVDKLEAKKAEEKS
                     A"
     gene            complement(8429..9310)
                     /locus_tag="VKN64_10145"
     CDS             complement(8429..9310)
                     /locus_tag="VKN64_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60644.1"
                     /translation="MEWSDIIKRLFTNKLSVFGIVIVFFFAIVAIFAPWIAPPADPDE
                     PYDIPRSGWKIAPTPPDADNIFGTTEGSYDIFYGMVWGSRTAFRIGFIVVGISTLIGI
                     FVGTISAYYGGWFDEIMMRITDIFMSIPFLVAALVLTAILGKGLDKVMVALIVFGWMG
                     TARLMRSQVLEIKAEEFIDAAYALGAGDTRIIFKHVLLNSIFPVLIQACMRMGSMVIT
                     AATLSFLGVGAPPGYADWGQMISFARNWIIGAQGNPLTYWYTLMIPGTAILLFSLSWN
                     LIGDAFRDILDPKLQQK"
     gene            complement(9320..10351)
                     /locus_tag="VKN64_10150"
     CDS             complement(9320..10351)
                     /locus_tag="VKN64_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60645.1"
                     /translation="MINYIVRRLLILPLILFGVTLLVFGMLMMLGPYQRVATYVQDPT
                     QLKGGDLDLLINKYGLDDPFYMQYGRWLDGLLHGDLGWSEAAGAPVVEAIFDRFPATL
                     ELAILAVIPVIMGGIWLGVFSAVHHNDFWDHTTRVFSTVGWSFPTFVFGLIILMIFYG
                     VLGWTPPGRLSNWALELKNTAQFTVFTDMNILDGILNGNISFALDSLRHLVAPVFTLA
                     VVWWAFILRITRSSMLETLRKDYIRTARAKGVKENIVINKHARRNALIPVVTVAGPMV
                     LGLLGGIVTVETVFGYRGLGLLAYETATRLDYAGILGIAMYFGGILVLINLIVDVSYA
                     FIDPRIRLE"
     gene            complement(10470..12284)
                     /locus_tag="VKN64_10155"
     CDS             complement(10470..12284)
                     /locus_tag="VKN64_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60646.1"
                     /translation="MKKKFGALLLSLILVFTVVSIAGAQDEVKSPDTYTHVTIGNQST
                     LDPHYSYDTASSEVIDNVYECLIQYQGESINKFSPLLATEVPSRENGLISEDGTEYTF
                     PIREGVKFSNGNDLTPEDVKYSFLRGMIMDRAYGPMWMIYEPLFGDISSLPGLTQEVV
                     GVEDPKELTAEQSAKVFAELDKKITIDGNEVTFHLENPYPPFLNILAQHAAWSSILDK
                     EWSIKQGAWDGSPETLADYHDPVKEDDPLYDKMMGTGPFVLTEWVNGDHVIFKRNDNY
                     WREPANFKTVIIKNIDEPTTRILLLKRGDADSIALDYQYFNQIEGDESIDIIRELPTL
                     QNLTMLFNWTIETKGNDYIGSGKLDGEGIPSDFFTDVHVRKAFSYAFNYKAFIEEVRQ
                     GQSQKLRGPIVNPLLGYDEGSPVYQLDLEKAEEHFKQAWDGQVWEKGFKLTILYNSGN
                     MTRKTAADIFKTYLEQINPKFDISTRAVQWSTFLDESARGTLPLQIGGWLADFPDPHN
                     FVQPFLHSAGYYGGKRGQNYIDWAKENVDPLIDKGITTMDQDERVDIYKEIQNIAHEQ
                     AIDVWIDQPYSARIQRDWVKGWYFNPMRPGQYFYILDK"
     gene            complement(12576..13160)
                     /gene="coaE"
                     /locus_tag="VKN64_10160"
     CDS             complement(12576..13160)
                     /gene="coaE"
                     /locus_tag="VKN64_10160"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMA60647.1"
                     /translation="MIIGLTGGIATGKSTVCKILKKLGIKIIESDKIAHQVLNLDEVI
                     EEVKKHFGETVLDKDGYIDRKKLGKVVFNEEKKRKQLESITHPKIFELIDKKIDEYQD
                     KKEEIIVLDAPLLFETALDKKVDETWVVYADKDTQIKRLEKRDDLNKQAAIKRIDAQI
                     PLKTKVKKADVVIKNEGTINELESKINKLVEKRR"
     gene            complement(13173..14000)
                     /gene="mutM"
                     /locus_tag="VKN64_10165"
     CDS             complement(13173..14000)
                     /gene="mutM"
                     /locus_tag="VKN64_10165"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HMA60648.1"
                     /translation="MPELPEVQTVVTGLNKMLRDKKIVNTHIYDEMVIGYPEKEDFIS
                     LSEGKKIKSLTRRGKYIIINFSDNKHKLVIHLRMSGKLLYKKRNDEKDKHTHVIFEFD
                     DDTDLRFNNIRKFGRLYFITENELEEAGNLSNLGIEPLSDEFTLDLFEEMVDGRKGMI
                     KPLIMNQEFIAGVGNIYADEALFMSNIRPDRKADTLSEQEIKDLYNAIRKILKKGIKM
                     GGTSVSDYVNALGKSGKFQHELNVYKKEGEKCEKCGEAIEKKKVSGRSARFCPNCQD"
     gene            complement(14015..>15212)
                     /gene="polA"
                     /locus_tag="VKN64_10170"
     CDS             complement(14015..>15212)
                     /gene="polA"
                     /locus_tag="VKN64_10170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMA60649.1"
                     /translation="LAQMETNGIYADCEFLEELSQQIADKIGEIEENTYELAGEEFNL
                     NSPKQLGEILFDKLGLPVIKKTKTGYSTSIDVLEKLEDKHDIIPLIMDYRKYQKIKST
                     YVDPLLELVDDKTGRIHTNFNQMVTATGRLSSTEPNLQNIPIRTEEGRKIRKAFIPEK
                     KDWVLLSADYSQIELRVLAHISEDEQLIKAFKEGQDIHTQTASEVFDVDKKEVTSNLR
                     RHAKVINFGIAYGMSSYGLAQDLDISNGEAEEYINRYFARFPGVKKYMDEVVNKAKKD
                     GYVRTIFNRLRYIKGINSSNYHRRSFAERAAINTPIQGSAADIMKIAMIEVYDALQSR
                     KDVKAKMLLQIHDEIVFEVAKSKLEEIAKLVKEKMEKAVELKVPVIVDLKTGNNLKEQ
                     AEYDIK"
BASE COUNT         4606 a         2997 c         2191 g         5418 t
ORIGIN      
        1 ccctggccag tatcaaacaa atatttttta tcaccatatt caaaataaaa agatagtcca
       61 tgttcagctc gtaaatttct cttataaaca gtattttcag atagtacttt tattttaagt
      121 ctgttactca tgataaatac ctcctttcaa ataaataact taaaaagata ttttttaaca
      181 taatattttg tatattaatg gtattcaaaa tttaaaaatc cttattttaa tacattttta
      241 aatactctat aggcattttt gtaaaaaatc ttctcaacct cctgctcatt ataaccattc
      301 tcaaataata tgttttttaa atttcccaac ttcctgatat cagcaagtcc tttcggtgta
      361 tcactaattc catcaaaatc tgtgcctaat ccaacataat ctatccccac taattttttt
      421 atataatcga tatgttcaaa aacatcatta atatcagctt tttcttgtgt atttaaaaaa
      481 agtgggtaaa aattaattcc aattactcca tttgctttag caattgcttt aatttgttta
      541 tcagttaaat ttcgcgggtg gtcacataat cttttaacat tagaatggga ggctattatg
      601 ggatcttcac ttatttccat cacatcccag aatccttttt catttaaatg ggatacatcc
      661 acaatcatat ttaaactatg catctctttc acaaaatctt ttccaaaact tgttaaacca
      721 cattttgaat taacccctac cccatcacca atctggttcc tatggttcca ggttaaggtt
      781 atgagtttaa tacctaaccg actgaaaact ctcaaagcgg ataagctata taggcttttg
      841 gccccttcta ctgctaaaat ggcagctatc ttatcttcat tattaatttt taaaatatca
      901 ttataatgtt ttgccaatac taattcttca caattttcta agatattata aaaatcatct
      961 accatttgaa tggttttctt taaaccatct cgatttgttt tataattttc ttctacatag
     1021 attgcaaata caattaattt aacttctgct tttttcaacc tcggtaaatc tatatgaaag
     1081 tctttattta aattgttgaa attatagttt ttattcttat atttatttaa aagagtatca
     1141 gtgtggatta ttgcagaact gctcacataa atacctccca ttttttaagt atagaaatct
     1201 cacaatttga atattgtgag attattagta ttaatataat gttgctctta tagagattga
     1261 aattcttcaa ctatctcctc tagcaattca ttatcattta gaatatcata tcccgttaaa
     1321 gccagggctt gagcagcttt aattaaggct tgctgacctt tttttgacat agtcgcatct
     1381 ctaaattcaa cggtatgtcc tatcaattca tcacctattt taatataagg gtgaattgat
     1441 ggtactacct gactgacatt tcccatatcg gtagaaccta agctttcttc cggatcagct
     1501 atattattaa ctcccgctaa ttttaaattt ttttcataag tggcagccag ttttccactg
     1561 gggtttaaat tatcatttga attttcaaaa ttactaaact ctacttcaca gccggtcatc
     1621 tgggctgctc cccgagcaat attttttcct ttttctatca cttcttttaa ataatctttt
     1681 tggcgggctc taaagtaaaa tttggcttcc gcaagatcag gcaccacatt ggggctttct
     1741 cctcccttac taataatccc atgtatacga acatcatctt taaggtgttc ccttatagca
     1801 ttaataccgt taaataactg aattactcca tctaaagcat tgatcccttt ttcaggggca
     1861 gcggccgcat gggcagtctg accgtgaaaa gtaaacttaa tagcttcgat tgccagagaa
     1921 gttgaatagg tcctgttttc attagaagga tgaaccatca ttgctgcatc tacttcatca
     1981 aagatgccct tttctaccag ggtaacttta cccccaccga cttcttcacc ggggcagcca
     2041 ataacctgga ttgtaccttt taaatcgttg tacttactta atgccacagc tgctcctacg
     2101 ctcattactc ctatcatatt atgaccgcag ccgtgtccta tattaggtag ggcatcatac
     2161 tcacaaagat aggctataac cggacctggt tcattaaaat catattcagc cttaaaggct
     2221 gtttctaagc ctccgatagg tttttttatc ttgaagttat ggctggctaa atagtctgtt
     2281 agccatttta cagccttaaa ttctttgaag cctaattcag ggtttttacc tattctatca
     2341 tttagattaa ttaaatcatc ttttatttct tctaaattat ttagtattgt atccttgtta
     2401 gccattttat cccacctatc tttatattta aattacttct tcgataaaca tcttagttaa
     2461 ctttggatca aactgaattc cggccatgga tcttaatcta tcaatagctc tttccttaga
     2521 aatcttttcc ttatatacct ggtcatgtgt cataacatca aaagcatcta ctatactgat
     2581 tatcctggca tataggggta tattatcgcc tgccttctgt tcgggatatc cagtcccatt
     2641 ccaccattcg tggtggtgta agatcgcctg ggcgatctca ttaacttcat tcagcgagtt
     2701 tgcaattcta aatccaacct ctggatgggt ctgaataatt ttatattctt cattagaaag
     2761 atcactttct tgattaatca attcccgcgg gatattaatc attcctatat catgtaagcg
     2821 ggctgtcttt ttgattaact taattttttc ttggtcaaat ttcaattgtc gggcaaattt
     2881 ttcagccaaa cttttcaccc gctgacagtg agactctgtt tcataaccca ttttatttaa
     2941 ttttttataa aggacctcca gcatctgttc actattttgc ttgtaatttt tagttttatt
     3001 attatacatc ctgcggtctg ccttatcaat tatatcaact atatctgttt ctttttctac
     3061 tttggtggca aaaccaaaag aaactgataa cggaatgtct ttataggttt tttgctcaag
     3121 tctatctttg attctatcta ttatactcaa tatattcttt ttggttgtgc cgggtaataa
     3181 gattaagaac tcatctccac cccagcgtcc taaaatgtct tcttttcggc atgcatcgtc
     3241 taaggtggct gcaatgcatt ttaaaagctt atcaccatca tcatgaccaa atgtatcatt
     3301 gattaatttt aagccgttaa cgtccgctat gatcacgctc acaggtaatt gccgactgtt
     3361 atttaatctt ttaatctctt cttcgatata atctctatta taaagccctg tcaagtaatc
     3421 gtgaaagcca aaatatctga tttcctcttc tctatttaat ctttttaaag ctgccccgga
     3481 atgggagatt agtaattcca ctaaattttt atcctggtta gtaaaataat catattcttc
     3541 tgagataatc tggaatgtcc catactgctg gatgggtact gtaagagcag atttatattg
     3601 ggtcttaacg ggtgaagcat ctctttcagc cggaatatta ttacatataa tcgttctctg
     3661 ttcgtgataa gcctttgtag ctaaactcat ttcatccaaa ctcattgaga tgacatcatc
     3721 atcagttaaa ttctgtgatt ttccaactgc cattaattta ttatccttaa ctatatctaa
     3781 agagatcaca tcatagtcta atatttcttc agctgcttct accgtttgct tatagatttc
     3841 ttctctttta gctgtgtttt ccatcttgat ggcaacatca tgcagctttt caatcttatt
     3901 tttgttttcc ataaccttat tttttaactt aatctgttct gttttatctt tcattgctat
     3961 gataacagcg tttttttcat taaaatccat gaaattaatg tcggttgcta aatattttag
     4021 ctcaccttta tcactttcag cccttaattc tatcggatat ccattacttt ttttaccttt
     4081 tttaattaaa tctgataatt tcttatacga atcttcagta aggatatctt ctaattttat
     4141 tttatctaag tgatcactgt caatattaaa tatttctcgg gccgacttat tcaagttcat
     4201 taaccttaaa tcatctttag ctaaaacaaa gaaagcatct gaggcattat caaagatact
     4261 cttaaaccgc tctccatacc ttttaatttc ctcattaata agttcaagct ggatattcat
     4321 aaattcatat gcgatagcga tagggaagat caaacctaaa agatataata aatcattcga
     4381 aaaaaatata gctaaaaatt catagataga ccaaaggata aaggttgtag aaaaaaacat
     4441 cttatgtaag ttattaatct ttttactttt agtaactaaa tatcctatat ctaaaaagta
     4501 ggtaactatt aagagttttg agaggacttc aatataatta ccccaattat tatattgaag
     4561 caatactaaa atggaaaata ggtaaattaa atgtaataaa ggccaaaatt tatttgattt
     4621 gtttagcagg tatttattca aggagtaatt aactaataaa gcggctgtag ttgacattat
     4681 ataagtataa attaaatatt cttgctttat agatagaaag gataacgcta tggaaataaa
     4741 aaacaatatt cccatgccaa taaaaaatat tgtatttata tcgctatatt ttctatattc
     4801 ttttttatta aaataataga caattattat aaaaaataga aaaataattg tcataatcat
     4861 taaatctatt atactcatcc tctaatctac tcctataata tttttaatta aaatacaaaa
     4921 ggatttccat cctatttgct gaataatata tataatggta aaggaggttt taacattgca
     4981 aacatttcaa tttgaaaatt tatataattc agccaaagga tatgtattaa actttttaga
     5041 taaatatctg ctatcatatt ataatttagc acaaattata tttctccttc ttttatttgg
     5101 aatcagtaac attttatcca aaaaatataa agaaagagta ataaatttaa taccagatac
     5161 atttgtaatt aaagaaacac tgggtgattt atttaaatta attatatatg tgattttact
     5221 gtggctttat ataataataa attcgatctt aaatttccct acttataccg ttatcatagt
     5281 agctaatctc ttaacagcct ggattattat aaatctacta tcagatttaa taaaaaatga
     5341 acttctctct aaaatatttt ctattttgat ctggactatt gcggcattac atgtactcgg
     5401 tatatatgaa gaaatagtaa atattttaag taaaatttca tttagaagtg gtaatatcca
     5461 catctcatta ttgatggtta ttaaaggtat attaactcta tttatattct tatggattgc
     5521 aatgaaatta tcctcctatt ccgaaaagca aattaagaag ttatctcctt tgaccccgtc
     5581 tattcgggtt ttattaacaa aattattgaa gtttgtaatt tttacagctg taattctaat
     5641 tactatgagt tctttaggta ttgatctcac agccctcgct gtagtcggtg gtgctgttgg
     5701 tgtcggggtt ggctttggac tccaaaaaat tgtctcaaat tttatcagtg gtattataat
     5761 cctattagat aaatcagtaa aacccgggga tgttattgaa attgataatg tttacggcca
     5821 gattaagtcc atcgggttaa ggtatatatc ggttttaacc ttagatggca aagaatatat
     5881 tattcccaac gaagatttaa ttaccaaaaa ggtgataaac tggtcttaca gcaacaatct
     5941 agttaggaca agtgtctctg tcggggtctc ttataatagt gatgtagaaa aggccatgga
     6001 actattgcaa gaagcaatag ctggtattga taggatacta tctaaacctc cacctaaaat
     6061 atttttaaat gagttttcag atagctcggt caatattgaa gttaagttct ggattagaga
     6121 cccggaaaat ggtattaata atataaaaag cgaagtaaat aaaagcatct ggaagctgtt
     6181 taaagaacat gatattgaaa ttccattccc ccagcaggat attcacttta aatctttaag
     6241 tccgcaggtt aaagaattct ttcaaaaaga atagttgaaa ataatttttt aacaaatctt
     6301 ctttttaaat ataaattaaa tatgtaagta tttaaaaaag cctctctcaa aaactgagag
     6361 aggcttttta tataccaatc tatataatat tttattcctg ttcaagaaga tgacaggcag
     6421 caaaatgttc atcgccgtaa tctttgaatt ctggttcttt ttcactacat acatccattg
     6481 catatggaca gcggggatgg aacctacaac cagatggtgg atctaccggt gaaggcacat
     6541 ctccttccag gattattctg tccgtctcat aagtaggatc tgctaccgga atcgctgaaa
     6601 gcagagactg tgtataagga tgtttcggat catcaaatag ttcttttttc gatgccactt
     6661 caactaattt accgaggtac attacagcta ctctatcact tatatgttta acaacactta
     6721 aatcatgtgc gataaataga taagttaaat caaagtcttt ctgtaagtcc tgcagtaagt
     6781 ttataacctg agcctgaatg gaaacatcaa gcgcagaaac cggctcatcc gcaattatca
     6841 agctagggtc aacagctaag gctcttgcaa caccgatacg ctgtctctgt ccaccactga
     6901 actcgtgtgg atatcttttc atatattcgg gacctaaacc tactgtgtca agtatatctg
     6961 ctaccttttc attacgttct tttttagatg aggcaagatt atgaatatct attggttccc
     7021 ccactatatc ggcaactgtc atccggggat ttagtgaggc ataagggtcc tgaaagataa
     7081 tctgcatctc ttttctcatt tcccttagtt ctttttcatc taaagataat aaattcttgc
     7141 cttcaaacca aatttctcct tctgtcgctt ccaggaggcg aaggattgta cggcctgctg
     7201 tagacttacc acaaccggac tcaccaacta atcccagggt ctctcccttt ttgatataaa
     7261 agcttatatc atcgaccgct tttacatggg cgacggtttt acgaaatact ccttctttga
     7321 ctgggaagta cttttttaag ttctttatct caagtaattt ttcttgctct tctgccaatt
     7381 cttttcacct ccattaagcg cttttctctt ctgctttttt agcttctaat ttatcaacat
     7441 gccagcaggc tgaataatgg ccctcttcaa cttcttccag ttctggctct tctgtacgac
     7501 atttatcggt tgcaaatgga catcttgtat tatacttaca gccgtccggg aagttcagtg
     7561 gattaggtac attaccgggg attgcttcta atctctctaa gtctttctca atgtgaggta
     7621 tagaatgcat taaaccccag gcatatggat gttttggatc agcgtaaagt gtttcaacat
     7681 cggcatattc cactatctta ccggcataca taactgcaac tctatcagat acctcagcaa
     7741 taacaccgag atcgtgagtg atcatcataa tcgccatatc aaactgatct ttcaggctat
     7801 tcatcagctc taatatctga gcctgaattg taacatccag ggcagtggta ggctcatcag
     7861 caatcaataa ttgagggtca caggataaag ccatcgcgat cattaccctc tgtctcatac
     7921 ctccacttaa ctggtgggga tattcatcga cacgttgttc aggtaaaggt attcctacct
     7981 tcctgagcat gttaatggcc tcttcacggg cctgtttttt gttgatatct ttatggataa
     8041 gcaatgcttc cataatctga tctcccacgg tataaaccgg gtttaaggag gtcattggct
     8101 cctggaagat cattgagata tcattacccc tgatctttct catctgagtt tgatttaatt
     8161 cagttaaatc tttaccatca aacaatattt cgccttcttc tatttttcca ggtggtgatt
     8221 caataagtct catgatagag agtgaggtaa cactcttacc acagccggac tcaccaacaa
     8281 ttccgagagt ttcacctgga tatatttcga agcttactcc atcaaccgct ttcacggttc
     8341 cttcttcagt atggaagtaa gtttttaaat tgttcacatc tataagcttt tctttactca
     8401 aatctatcac ctactttatc taatttaatt atttttgttg aagcttagga tcgagaatat
     8461 ctctaaatgc gtcaccaatt aagttccagg ataaactaaa taataagatc gctgtaccgg
     8521 gaatcattaa agtataccaa taagttaatg gatttccttg tgcaccaata atccagttac
     8581 gggcaaagga gatcatttga ccccagtctg catatcctgg aggggcacca acacctaaga
     8641 aacttaaggt tgcagcagtg ataaccattg aacccattct catacaagcc tgaattaaaa
     8701 ctgggaaaat actatttaaa agtacgtgtt tgaagataat tctagtatct cctgctccca
     8761 gggcatatgc tgcatctata aactcttccg ctttaatttc taaaacctga gaacgcatta
     8821 aacgggccgt tcccatccag ccgaagacaa tcaaagcaac cattacttta tcgagccctt
     8881 ttcctagtat ggccgtcaac accaaggccg caaccaagaa aggaatcgac atgaagatat
     8941 ccgttatccg catcattatt tcatcgaacc agcccccgta ataggccgat atagtaccga
     9001 caaatatacc tatcagggta cttattccaa caactataaa accgattcta aatgcggttc
     9061 ttgaacccca taccatacca taaaatatgt cataagagcc ttcagttgtt ccaaatatat
     9121 tatcggcatc cggtggggtt ggagcaattt tccagccgct tctaggtata tcataaggct
     9181 catcgggatc agctggtgga gcgatccagg gagcgaagat agctactatt gcaaaaaaga
     9241 aaactatgac tataccaaat actgataatt tatttgtaaa taatctctta attatatctg
     9301 accattccat atatttacat cactccaatc taattctagg atcaatgaaa gcatatgata
     9361 catccactat taagttgata agtaccaaaa tacctccaaa atacatcgct attcctagaa
     9421 tacctgcata atctaatctg gtcgctgttt catatgctaa taaacctaat cctctatagc
     9481 cgaagaccgt ttctactgtt actataccac ctaataatcc taataccata ggaccagcaa
     9541 cagtaactac agggattaaa gcatttcttc tagcatgttt atttataact atattttctt
     9601 ttacaccttt tgctctagca gtacgtatat aatctttacg taaggtttct aacatactag
     9661 atcttgtaat ccttaatata aaggcccacc aaacaacagc taaagtaaat acaggtgcta
     9721 ctaagtgtct taaagaatct aaagcaaaac taatattacc gtttaatatg ccatctaata
     9781 tattcatgtc ggtaaagact gtaaactgtg ctgtattttt taactccagg gcccagttag
     9841 ataatctacc cgggggcgtc cacccgagaa ctccataaaa tatcattaaa attattaatc
     9901 caaatacgaa agtagggaac gaccaaccta ctgttgagaa caccctggta gtatgatccc
     9961 aaaagtcatt atgatgaaca gccgagaaca caccgagcca aattccaccc attataacgg
    10021 gtatcaccgc cagaattgcc agctccagcg tggccgggaa ccgatcgaaa atagcttcga
    10081 caaccggagc accagctgcc tcagaccatc ctaaatcacc atgtaataat ccatctagcc
    10141 accttccata ctgcatatag aaggggtcat ctaaaccata tttattaatt aataaatcca
    10201 ggtcgccacc cttaagctga gtgggatcct gaacataggt agcaactcgc tgatacggtc
    10261 ccagcatcat taacatacca aagaccagca gggttactcc aaataatata agcggcagta
    10321 ttaatagccg tctcactata taatttatca actgttaaaa ctcctttctt ccattactaa
    10381 aaatttacaa atgtcctgtt ttcatagaat tgccaggggg ttacccccct ggcattatcc
    10441 tttaatacta aattgtaatc aaactactat tacttatcta agatatagaa gtattggcct
    10501 ggacgcattg gattgaagta ccatcctttg acccagtcac gttgaattct agcactatat
    10561 ggctggtcaa tccagacatc aatagcttgt tcatgtgcaa tattttgaat ttctttataa
    10621 atatctacac gttcatcttg atccatagta gttatacctt tatcgattaa tggatctaca
    10681 ttttctttag cccagtcgat ataattctgt ccacgtttgc ctccataata acctgcggag
    10741 tgtaagaaag gttgaacgaa gttatgtgga tctgggaagt cagctaacca accaccaatt
    10801 tgaagtggta aggtacctct agctgattca tctaagaatg tagaccattg aactgctctt
    10861 gtactgatat caaacttagg attaatttgt tctaagtatg tcttaaagat atcagcagca
    10921 gtttttcttg tcatgttacc agagttatat agaattgtga gtttgaatcc tttttcccaa
    10981 acttgtccat cccaggcttg cttgaagtgt tcttctgctt tttcaagatc taattgatat
    11041 acaggagacc cttcatcata gcctaataga ggattaacaa ttgggccgcg aagtttttgt
    11101 gattgtccct gtcttacttc ttcgatgaat gctttgtaat tgaaagcata actaaatgct
    11161 tttcttacat gaacatctgt aaagaagtca gaaggaatac cttcgccatc aagttttcca
    11221 ctaccaatgt agtcattacc tttagtttca attgtccaat tgaataacat tgtcaagttt
    11281 tgtagagtag gtaattctct gatgatgtca atgctttcat cgccttcaat ctgattgaaa
    11341 tattgatagt ctaatgcgat tgagtcagca tctccacgtt ttagaagtag gatacgtgta
    11401 gttggctcat caatattctt aatgattaca gtcttgaagt tagcaggttc acgccagtag
    11461 ttatcatttc ttttaaagat tacatggtcg ccatttaccc attcagttaa gacaaatgga
    11521 ccagtaccca tcattttatc ataaagtggg tcatcttctt ttactggatc atgatagtca
    11581 gctagagttt caggactacc gtcccaagca ccctgcttaa tactccattc tttgtcaaga
    11641 atacttgacc aagctgcgtg ttgagcaaga atgtttaaga atggaggata aggattctct
    11701 agatggaatg taacttcatt accatcaata gtaatcttct tatctaattc agcaaatact
    11761 ttagcagact gttctgctgt taattcctta ggatcttcta cacctacaac ttcttgtgta
    11821 agtccaggta aagaagagat atctccaaag agtggttcat aaatcatcca cattggacca
    11881 taagcacggt ccatgatcat acctctaagg aaactatact taacatcttc aggagttaaa
    11941 tcattaccat tactaaattt aacgccttca cgaattggaa aggtgtattc tgtaccgtct
    12001 tctgagataa gaccattttc tctggaaggt acttcagttg ctagtaatgg tgagaattta
    12061 ttaattgatt cgccctgata ttgtatcaag cattcataaa cattatcaat aacttctgaa
    12121 ctagcagtat cataagaata atgtggatcc aaagtgcttt gattacctat agttacgtgg
    12181 gtataagtat ctggactttt tacctcatct tgtgctcctg cgatactaac tactgtaaaa
    12241 actaagatta atgacaacaa taaagctcca aattttttct tcatttaaaa tttttccctc
    12301 cttgaaataa tactaactta tttgaaaaat ttactggtac tgttcaattc tatcgtcctc
    12361 tgaattcacc ccctttaaaa tatagaaaaa attattttgg tcttaaataa gaccaataaa
    12421 acttagatga cagaactctc tgtaaaactc tattactata ttctatgaat tagtttgaaa
    12481 tccttcctta tactacaaaa aattcttgat accattggcc ttatataagc aatctaagaa
    12541 tatttatttg cttttgctta taattatata acaaactatc tccttttctc aaccaattta
    12601 tttattttac tttccaattc atttatagtc ccttcatttt tgattactac atctgctttt
    12661 tttacttttg ttttaagagg tatctgggca tctattcttt ttatagctgc ttgtttattt
    12721 aaatcgtccc tcttttctaa ccttttgatt tgtgtatctt tatcagcata aacaacccag
    12781 gtttcatcca cttttttgtc taaagcggtt tcaaaaagca atggtgcatc taatactatt
    12841 atctcttctt tcttatcctg atattcatct atttttttat caattaattc aaatattttg
    12901 ggatgggtta tagattccaa ctgttttctt ttcttttcct cgttaaaaac aacttttcct
    12961 aacttttttc tatctatata gccatcttta tctaatacag tttctccgaa atgtttttta
    13021 acttcttcaa taacttcatc taaatttaaa acttgatggg ctattttgtc agactcaata
    13081 attttgatac caagtttttt tagtatctta caaactgtac ttttaccggt tgcaattccg
    13141 cctgtaagtc caataatcaa atttaaatcc ccctaatctt ggcagtttgg gcagaatctt
    13201 gctgaacgcc cgctaacttt tttcttttct atagcttctc cacacttttc gcacttttct
    13261 ccttcttttt tatacacgtt cagttcgtgc tgaaacttcc cacttttacc aagggcattt
    13321 acatagtcac ttacagacgt accacccatc tttatacctt tttttagtat ctttctgatc
    13381 gcattatata aatctttaat ttcttgctca gataatgtgt cagcttttct atccggcctt
    13441 atattggaca taaaaagtgc ttcatcagca tagatattac caaccccggc gatgaattcc
    13501 tggttcatta tcaagggctt aatcatgcct tttctaccat ctaccatctc ttcaaaaaga
    13561 tctaaagtga actcatcact taaaggttct atacctaaat ttgataaatt gccagcttct
    13621 tctaactcgt tttctgttat gaaatataat cgaccaaatt ttcttatatt attaaatctt
    13681 aaatcagtgt catcatcaaa ttcaaatatc acatgggtat gtttgtcttt ttcatcattt
    13741 ctttttttat atagtaattt acctgacatt ctcaaatgta tcactaattt atgtttatta
    13801 tcactaaaat ttattattat atatttaccc cttcttgtta aggattttat ttttttccct
    13861 tcacttaaac ttataaagtc ctctttttcg gggtaaccaa ttaccatttc atcatatata
    13921 tgggtattaa ctatcttttt atcacgtaac attttattta agcctgttac aacggtttgt
    13981 acttcaggca attccggcat tttatccacc tcaattattt tatatcatat tcagcttgtt
    14041 cttttaaatt attcccagtt tttaaatcta ctattaccgg tacttttaac tctactgcct
    14101 tttccatctt ttctttgact aatttagcaa tctcttctaa tttggattta gcgacttcaa
    14161 agacaatttc atcatggatt tgtaaaagca ttttggcctt tacatctttt ctagactgta
    14221 aagcatcata aacttctatc atggctatct tcataatatc agcagcacta ccctgaatag
    14281 gtgtattgat agcggccctt tcagcaaatg atctcctatg ataattacta ctatttatgc
    14341 ctttaatata tcttaaacgg ttaaaaatgg tccgtacata gccatctttt tttgccttgt
    14401 ttacaacttc gtccatatat ttttttacac cgggaaatct tgcaaagtat ctatttatat
    14461 actcttctgc ttctccattt gatatatcta aatcctgagc tagaccataa gaactcattc
    14521 cataagctat accaaagtta attactttag catgtcttct caaattagaa gtaacttctt
    14581 ttttatcaac atcaaagact tccgaagcag tctgagtatg gatatcttgt ccttccttaa
    14641 aggctttaat caactgttca tcctcactga tatgggctaa tacccttaat tctatctggg
    14701 aataatcagc cgataatagt acccaatctt ttttttcggg aatgaaagct ttcctgatct
    14761 ttctaccttc ctcggttcta ataggtatat tttgtaaatt gggttcagta ctacttaagc
    14821 gcccagtggc ggtaaccatc tggttaaaat tagtatgaat tctacctgtc ttatcatcta
    14881 ctagctctaa taaaggatct acatatgtag acttgatttt ctgatacttc ctataatcca
    14941 ttattagagg tataatatcg tgtttatctt ccagtttttc taatacatca atactggttg
    15001 aataacctgt tttagtcttt ttaataacgg gtagacctaa tttatcaaat aatatttcac
    15061 ctaactgctt aggagagttg agattaaatt cttctcccgc cagttcataa gtattttctt
    15121 ctatctcacc aattttatca gcaatttgct gtgaaagttc ttctaaaaat tcacagtctg
    15181 cataaattcc attggtctcc atttgggcta aa
//
LOCUS       DATGCJ010000228         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_436654, whole genome shotgun sequence.
ACCESSION   DATGCJ010000228 DATGCJ010000000
VERSION     DATGCJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436654"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /gene="nadE"
                     /locus_tag="VKN64_10175"
     CDS             <1..450
                     /gene="nadE"
                     /locus_tag="VKN64_10175"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HMA60650.1"
                     /translation="EEQESQANFALANAKPRLRMVTLYYYAAQNNYLVLGTDNWSELK
                     VGYFTKYGDGGIDLTILGRLVKTEVRELAEHLVVPQEIIDKKPSAGLWQDQTDEDEMG
                     LTYEELDRYILTGEAEPEVKEKIENLSSKNSHKLEPIPIPTREILLT"
     regulatory      497..683
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            780..1391
                     /gene="cobU"
                     /locus_tag="VKN64_10180"
     CDS             780..1391
                     /gene="cobU"
                     /locus_tag="VKN64_10180"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HMA60651.1"
                     /translation="MSQSFLILGGARSGKSSFAEKIAENLGETEVVYLATAQALDQEM
                     EDRIKHHQNQRPKDWKTVEEPIEVNKVLESIEKGKTVLMDCLTLYISNMILKTEELND
                     DENNLEQKVNIEKEIIEEIKKIITTAQARDLNLIIVSNEVGQGVVPNNELGRLFRDIA
                     GRANQFTADKVDKVFLTVAGFPLDIKDIGLRNKSDFLENLKED"
     gene            1393..>2254
                     /locus_tag="VKN64_10185"
     CDS             1393..>2254
                     /locus_tag="VKN64_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HMA60652.1"
                     /translation="MKKSIMFQGTASGVGKSLMAAALCRLLYQKGYKVAPFKSWNMAL
                     NSYVTKNGGEVGRAQALQAEACNVDIEVDMQPFLVKPKGNGQAQVIKRGKPLGDFAGQ
                     YKDDDYVKEALKTIKNSINNLYKKYDVLVMEGAGSPAEVNIQKRDLANMKVANLNDTP
                     VILVADIDKGGVFASLVGTMELLGEDRRLVEGIIINKFRGDKDSLQSGLEFIENKTGV
                     PVLGVMPYLKEINLPEEDSVTVKEYLKEQAEIKIGIINLPHLSNFTDFDVLGLEPAVE
                     LTYLEYGQEIS"
BASE COUNT          761 a          358 c          518 g          617 t
ORIGIN      
        1 gaagaacaag aaagtcaagc caattttgct ttagcaaatg ctaaacccag actgcgtatg
       61 gtgacccttt attactatgc cgctcaaaat aattatctgg ttttaggtac cgataactgg
      121 agtgaactga aagtaggtta ctttactaaa tacggtgacg gtggaattga cttaaccatc
      181 ctcggtcgtt tagttaaaac agaggtgaga gagttagcgg aacacctggt agtcccccag
      241 gagattatcg ataaaaagcc ctcagccgga ctctggcaag accagaccga tgaagatgaa
      301 atgggcctta cctatgaaga attagataga tatatcttaa ccggtgaggc agaacctgag
      361 gttaaggaaa aaatagaaaa tttatcttct aaaaacagcc ataaacttga accaattccg
      421 atcccaacca gagaaatttt gttaacttag caggagaatt ttaaaaagta tagtattata
      481 ttattaagag aacaatttaa tattgttata aataggtgct ttcagttact actggaagag
      541 aatagggaat caggtgcaaa tcctgagcgg gcccgccact gtgagcagct agtcgcccaa
      601 ataaccactt gtttttaaca aggaaggttg ggttagacgt taagctgtga gtcaggagac
      661 ctgcctattt atactctaca ttcttcggtt tcaagatcag tagatgcaag ggatatcacc
      721 agatctttag ctttaagcgg aggtctggtt tttttatttg tctaaaaggg gtgaaaaaat
      781 tgagtcaatc atttttaatt ctaggaggag cgcgcagtgg taaaagttct tttgcagaaa
      841 aaatcgctga aaacctgggg gaaacagagg ttgtttatct ggctaccgcc caggctttag
      901 accaggagat ggaagataga atcaaacacc atcaaaacca gagacctaaa gattggaaaa
      961 cggtggaaga accgattgaa gttaacaagg tattggaaag tatagagaaa ggtaaaactg
     1021 ttttaatgga ctgtctcacc ttatatattt ccaatatgat attgaaaacg gaagaattaa
     1081 atgatgatga aaataattta gagcagaaag tcaatattga aaaagagatc atagaggaaa
     1141 ttaaaaaaat tattaccact gcccaagcca gggacctgaa cttaattata gtttccaacg
     1201 aggttggtca gggtgttgtt cccaataatg aactgggccg gttattcaga gatattgccg
     1261 gccgagctaa tcagtttacg gccgataaag ttgataaggt atttttaaca gtagccggtt
     1321 ttccccttga tataaaagat attggcttaa gaaataaatc agatttctta gaaaatttaa
     1381 aggaagatta agatgaaaaa gtctatcatg tttcagggta cagcttcagg tgttggtaag
     1441 agtttaatgg cagctgcttt atgccgactt ttataccaga aaggttataa agtagcgcct
     1501 tttaaatcat ggaatatggc tttgaattcc tatgttacta aaaatggagg agaagtgggt
     1561 agagcccagg ctctccaggc agaagcctgt aatgtagata ttgaagttga tatgcagccc
     1621 tttttggtaa agccgaaagg taacggccag gcccaggtta tcaaacgggg taaacctctg
     1681 ggagattttg ccgggcaata taaggatgat gattatgtaa aggaagcttt aaagaccata
     1741 aaaaattcta tcaataattt atacaaaaag tatgatgtcc ttgttatgga aggggccggc
     1801 agtccggctg aagttaatat tcaaaagcga gatctagcta atatgaaagt agccaaccta
     1861 aatgatacgc cggtcatttt agtagcagat attgataaag ggggcgtctt tgcttccttg
     1921 gtaggtacca tggaactgtt aggggaagac cgccgacttg tagagggtat aattattaat
     1981 aaatttcgcg gtgataaaga cagtctccag tcaggacttg aatttattga aaacaagacc
     2041 ggtgtgccgg tattgggagt aatgccctat ttaaaggaga tcaacctgcc ggaagaggat
     2101 tcagtaactg tgaaagaata tttaaaggaa caggctgaga taaagatcgg gataattaat
     2161 ttaccacatt tatctaactt tactgatttt gatgttttgg gattagaacc agccgttgaa
     2221 ctaacttatt tagagtacgg gcaagaaata tcta
//
LOCUS       DATGCJ010000229         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_348523, whole genome shotgun sequence.
ACCESSION   DATGCJ010000229 DATGCJ010000000
VERSION     DATGCJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348523"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..175
                     /locus_tag="VKN64_10190"
     CDS             5..175
                     /locus_tag="VKN64_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HMA60653.1"
                     /translation="MVDVTDIDDVKIGDEVVLIGKQGDEEFSATEMADIIGTINYEIT
                     CDITKRVPRIYK"
     gene            643..1161
                     /locus_tag="VKN64_10195"
     CDS             643..1161
                     /locus_tag="VKN64_10195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60654.1"
                     /translation="MGKYIKNSNKIQIRETEISDIDYVRTVESAPANRENVFQWSKEQ
                     HIKIIEGKDTLHLIIEDQSFQKVGYAIIAGLESKNKSIELRRVVIDKKNKGYGTEFLK
                     LMKEYTFEDLEFHKLWLDFFDTNSRVETLYKKNGFKREGVLRDKYYYDNRYITMVVMS
                     ILEDEYFAEDRN"
     misc_binding    1222..1430
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1503..>2656
                     /gene="serS"
                     /locus_tag="VKN64_10200"
     CDS             1503..>2656
                     /gene="serS"
                     /locus_tag="VKN64_10200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMA60655.1"
                     /translation="MLDRKFIRENIDKVEKALKDRGVESPLDRFKKLEEERRDLLYEN
                     EQLRHKRNVNSDKIGELKREGKDASHLIDEMKEVSEKIKEYDEKLAEIEDELDSILLR
                     IPNIPHESVPVGTDEEDNREIKKWGEPREFDFDYKAHWDIGEDLDILDFERGGKVTGA
                     RFTFLKNAGARLERSLINFFIDIHTKEHDYKEVFPPFIANQESMTGTGQLPKFEKDMF
                     KLNGLPYYLIPTAEVPVTNMYREEILDKEDLPIYHTAYSACFRAEAGAHGRDTRGIIR
                     QHQFNKVEMVKFVEPETSYDELEKITQDAEELLQMLELPYRIVTLCSGDLGFSAAKTY
                     DLEVWMPAYDTYREISSCSNFLDFQARRAGIRYRPEPDASTKYVHTLNGSG"
BASE COUNT          983 a          345 c          527 g          801 t
ORIGIN      
        1 atttatggtt gatgtaacag atattgatga tgttaaaata ggagatgaag ttgttttaat
       61 aggtaagcaa ggagatgaag aattttctgc gacagagatg gctgatataa tcggaacaat
      121 aaattatgaa attacctgtg acataacaaa aagagtaccc aggatataca agtaaataaa
      181 atacaaaaaa ggaggtgggt gcccacctcc ttttacatta aatttattca tttttcttgt
      241 tttcaaagaa atctctgaag tctctactac cacttacatc ttttttaatt ccaaaaaaga
      301 aatctctaaa atctctacta ccgtgatcaa acatctatat cactcctttt atattttatg
      361 atataattta gtttattttc ttgagctaaa tagattagcg aagaaataag cattaaataa
      421 gttataataa ttatcatatt ctttataata tctgtcttca tcatttttat tattgttgta
      481 aagctcattg atataattat tctcgcgatt gaacatttat atcactcact ccccatatat
      541 ataatatctt tcttatttat tttcactatc tctattttaa ctaaataaat ccgagtaaaa
      601 tagtaagcat ttaacaaatt ttagcataga tgaggtgttt ttatgggaaa atatattaag
      661 aattccaata aaatacaaat aagagaaact gagataagtg atatagacta tgtgcgaaca
      721 gtagaaagtg caccggctaa tagagagaat gtatttcaat ggtcaaaaga acagcatatt
      781 aaaataattg aaggaaaaga tactctccac ttaattattg aagatcaatc ttttcaaaaa
      841 gttggctatg caataattgc ggggttggag agtaaaaata aaagtattga gttaagaaga
      901 gtggtaatag ataagaaaaa taagggatat ggaactgaat ttttaaaact gatgaaagaa
      961 tatacctttg aagatttaga gtttcataag ttatggctgg acttttttga taccaattca
     1021 agggtagaga ctttatataa gaaaaatggt tttaaaagag aaggtgtgct aagagataag
     1081 tactattatg ataataggta cataacgatg gttgtcatgt cgatcctaga agacgaatac
     1141 tttgcggaag acaggaatta atttctataa tttattgcat ttataataaa tatatattat
     1201 aataagtaaa aataaaatta tgataaatag ttgattggga aaagtaaatt atatttcttt
     1261 ttgagaaagt taatggccgt tgagaattaa caggaagtat aattgaatcc acccatgata
     1321 ggcaggggga aatcaatagt aactctgccc ggcgcagacc gttattcttg taagtggacc
     1381 ataatggtta ataagggtgg caccacgggt attatactct cgtccctact gtagggaatg
     1441 agggtttttt tatttttata taaacttttc atccaaaaaa aattaagcag ggaggaatta
     1501 taatgttaga tcgtaaattt attagggaaa atattgataa ggttgagaag gctttaaaag
     1561 acaggggagt tgaaagtcct ttagacaggt ttaaaaaact ggaagaagaa agaagagact
     1621 tgctatatga aaacgaacag ttaaggcata agaggaatgt taactctgat aagatcggtg
     1681 aattaaaaag agaagggaaa gatgccagtc atttgatcga tgagatgaaa gaggtttctg
     1741 aaaaaataaa agaatatgat gaaaaattag ctgaaattga agatgagtta gacagcatat
     1801 tattaagaat accgaatatt ccacatgaat ctgtcccggt tggtacagac gaggaagaca
     1861 accgtgaaat taaaaagtgg ggtgaaccaa gagaatttga ttttgattat aaggcccact
     1921 gggatattgg tgaagattta gatatcttag actttgaaag agggggaaaa gtaacggggg
     1981 cccgttttac attcttaaaa aatgccgggg ctagattgga aagaagtttg atcaactttt
     2041 ttatcgatat tcataccaaa gaacacgact ataaagaagt atttccaccg tttatagcaa
     2101 accaggaaag catgactggt actgggcagt tacctaaatt cgaaaaagat atgtttaagc
     2161 taaatggttt accatattac ttaatcccaa cagctgaagt gccggtaact aatatgtata
     2221 gagaagagat cttagataaa gaagacctac cgatatatca cactgcttac agtgcctgct
     2281 ttagggcaga agctggtgca cacggtagag atacaagggg aataatcaga cagcaccaat
     2341 tcaataaggt tgagatggtg aagtttgtgg aaccggaaac ttcctatgat gaattagaaa
     2401 agattactca agatgctgaa gagctgcttc agatgcttga attgccatat aggattgtta
     2461 cactttgtag tggagatcta ggtttctcag ctgccaaaac ttatgactta gaagtatgga
     2521 tgccggctta tgatacctat cgtgaaatat ccagctgtag taacttttta gactttcagg
     2581 ccagaagggc tggtattaga tacagacccg aaccagatgc aagtactaaa tatgttcata
     2641 ccttaaatgg gtctgg
//
LOCUS       DATGCJ010000230         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_211812, whole genome shotgun sequence.
ACCESSION   DATGCJ010000230 DATGCJ010000000
VERSION     DATGCJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211812"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VKN64_10205"
     CDS             complement(<1..715)
                     /locus_tag="VKN64_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMA60656.1"
                     /translation="MNYGYCNKALRIDLSNNLIKKEKLDEEFLKKYLGGRGLNVRRLY
                     DEVPPEVDPLSTENKMFIGVGPLTGTLFPGAARVNFTAKSPQTKILGDSNTGGFLGPE
                     IKFAGFDQIIIEGKADQLSYIYIKDGEAEIKSAQHLKQLDTFETQKKIKEELGDNRVQ
                     TAVIGPAAENGVKYSGIFCNMVRAAARTGMGLVLASKNIKAIVVRGTTDLGASKPDKF
                     KQLIDKMDEEIKNHSEYEIR"
     gene            complement(712..1437)
                     /locus_tag="VKN64_10210"
     CDS             complement(712..1437)
                     /locus_tag="VKN64_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HMA60657.1"
                     /translation="MYDLKIVVESIEGFCDLPMEIGDYFTVSGGKIEIPEGKHMCLWA
                     LQSLMPVLPAKQRELNEENDWIPHTKRFICPDPNGRVIFRIDRLDPKTGKPLEEEIEI
                     PPRILVNPDACTGCRACETVCAFVHTQSFNALRSRIKIEKNEIKGEDIPYICRQCGDA
                     PCIKACPVEALSRSPKTNAILVDEDKCIGCNKCAEACPFDAISFNENNVALICDLCDG
                     DVECVQRCPTDALKFDNKGGRKK"
     misc_binding    complement(1525..1792)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2048..>2603)
                     /locus_tag="VKN64_10215"
     CDS             complement(2048..>2603)
                     /locus_tag="VKN64_10215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HMA60658.1"
                     /translation="LIFLLFPALIILSYYISVSLDQVYPKSEASGLYYFIPFIFFSVM
                     FTGGPIQEEFGWRGYALPRLQEKLSPFFSSIILGFIWAIWHWPQFLVPEELTGMFYIT
                     PLWSFILTVISANFIYTWIYNNTNGSILAVLILHTQMNLFLWIFPVLYTQYGYLIILV
                     TFTIAASVVILLDKKQFFNKVRRY"
BASE COUNT          827 a          530 c          372 g          874 t
ORIGIN      
        1 ttcttatctc atattcggag tggtttttta tttcctcatc catcttatcg attagttgtt
       61 taaatttatc tggtttactt gcaccaagat ctgttgtgcc ccttaccaca attgctttta
      121 tatttttact tgctaacact aaccccatac cggttcttgc agctgccctt accatattac
      181 agaaaatacc ggaatactta accccatttt cagcagctgg tccgataaca gcagtctgta
      241 cacggttgtc acccaattct tcttttatct tcttttgggt ctcaaaggta tctaactgtt
      301 ttaaatgttg ggctgattta atttcagctt caccgtcttt aatatatatg taagataatt
      361 gatcagcctt accctctata attatctgat caaaaccggc aaatttaatc tctgggccca
      421 aaaatccacc tgtatttgag tcacctaaaa ttttagtctg aggtgatttg gctgtaaagt
      481 tcacacgggc tgctccagga aataaggtac ccgttaaagg accaactccg ataaacatct
      541 tgttttctgt acttaaagga tcaacttcag gaggtacttc atcataaagg cggcgtacat
      601 ttaaccctcg tcctcccaga tattttttta aaaattcttc atctaatttt tcttttttta
      661 ttaaattatt tgaaaggtct attcttaaag ccttattaca gtatccgtag ttcatttttt
      721 tctacctcct ttattatcaa acttcaaggc atctgttgga catctctgca cacattctac
      781 atctccatca cagagatcac agatcaaagc cacattattt tcattaaaag aaatcgcatc
      841 aaagggacag gcttcagcac atttgttaca cccaatacat ttatcctcat caactaatat
      901 tgcattggtt ttggggcttc tacttaaggc ctcaacagga caggctttaa tacaaggagc
      961 atctccacac tgtctacaga tatatggaat atcttcacct ttaatttcgt ttttttcgat
     1021 ttttatacgc gatctcaaag cattaaagct ttgagtatgt acaaaagcac agactgtttc
     1081 acaagcccta cagccagtgc aggcatctgg gttaaccagt atccttggcg gtatttctat
     1141 ttcttcttct aacggtttac ctgttttggg atccagccta tcgatcctga aaataaccct
     1201 tccattaggg tcaggacata taaatctttt ggtatgtgga atccagtcat tttcttcatt
     1261 taattctctc tgttttgctg gcagtaccgg cattaaactt tgcagagccc acaaacacat
     1321 atgtttacct tccggtattt ctatcttccc cccactgacg gtgaaataat caccaatttc
     1381 cattggtaaa tcacaaaaac cttctatact ttctacaaca atctttaaat catacaaaat
     1441 aagcaactcc cttcttttgg tttctaatgt aatatatata attaaaaaaa ggtggcacac
     1501 gaaataaaaa aacccttcac cccgcttagg gacgaaagat taataattcc gtgttgccac
     1561 ccttgttagt taattaatat taactcactt tattaaaaac catctattaa aaaatgattt
     1621 ttatttgcta ctaacgggca aaaaccggca caggataatt tatcatatca aagatatttc
     1681 cccctgcttc tcacaaggcc attctcttta ttttttcatc accggaatct cagctctgtc
     1741 ggctctctgt aaatgaaatc ataaagatac ttttcttggt caaagaattt aatcatatgt
     1801 tattgataat gattttacca ccgagaagta ataatgtcaa gaaaaaattt ccagatgata
     1861 gtatctaatt aatttggaat tttagataga cccctttcta tattgattta aatggaataa
     1921 aaccttatta actctatatt ctgttaacct tcccactttt taaagctatt aacacctctt
     1981 tagctatatg taacaaacaa gttaagagaa aatatgccct ttattattgt tatgatatct
     2041 tttaaattca atatcttctc actttattaa aaaattgttt tttatctaat aaaataacca
     2101 cactagctgc tatcgtaaat gtcactaaaa taataagata tccatattgg gtatataaca
     2161 ctgggaatat ccatagaaat aaattcatct gcgtatgaag gataaggaca gccagtatac
     2221 ttccgtttgt attattatat atccaggtat agataaagtt agctgaaata accgtcaaaa
     2281 taaaagacca aaggggagtt atataaaaca ttccggttaa ctcctctgga actaagaatt
     2341 gtggccagtg ccagatcgcc caaataaatc ccaagattat tgatgagaag aatggtgata
     2401 atttctcttg caatcttgga agtgcataac ctctccagcc aaattcttct tgaatgggcc
     2461 caccagtaaa cattacactg aaaaaaataa atgggataaa ataatacaaa ccagacgctt
     2521 cagattttgg gtaaacttga tcaagactta cacttatata atacgataaa ataattaggg
     2581 ctgggaagag aagaaaaatc aaa
//
LOCUS       DATGCJ010000231        10132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_348657, whole genome shotgun sequence.
ACCESSION   DATGCJ010000231 DATGCJ010000000
VERSION     DATGCJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348657"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VKN64_10220"
     CDS             <1..1069
                     /locus_tag="VKN64_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HMA60659.1"
                     /translation="NPVNNVNNLSKDAVMVISDRGYLKVERAWKKEIAEKIKCRFVLV
                     NTNTIVPVEIASDKEEYAAYTIRKKINNKVERFIDVVDKEKLKVNSLRLNIKGADLND
                     IDNLVNRLSIDKTVKPSPAFKGGSSEALNRLERFMGKNLKLYEEHGSNPDYDVTSKLS
                     PYLHFGQISPAYIYKRIKWNDIKAEDFLEQLIVRRELSFNYIYYNDNYNQKLTDILPD
                     WAVETLEEHESDKREYIYSREQLENYQTHDQYWNAAQKEMVLSGYMNGYMRMYWGKKI
                     LEWTQDPQKAFDIILYLNNKYFLDGRDPNGYAGAAWIFGKHDRAWQERQIFGKVRYMN
                     KNGLERKFDMEDYLKKVNNLN"
     gene            1216..1704
                     /locus_tag="VKN64_10225"
     CDS             1216..1704
                     /locus_tag="VKN64_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HMA60660.1"
                     /translation="MREKIFDILSYLIEELLNSGDEVVDQEDLINELVDKGYEIEDID
                     EAFQLIFEKPEIIEHSDIEGISEDNFLNRVFTVQEKMYLPKEHRGLIKRLLIMNIITI
                     KETEEIVNKIIQNVYYNNNKNFDFWELIQDVVDDENRINYISNHFKEFSTKLSKDFKY
                     IN"
     gene            1728..3782
                     /gene="topA"
                     /locus_tag="VKN64_10230"
     CDS             1728..3782
                     /gene="topA"
                     /locus_tag="VKN64_10230"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMA60661.1"
                     /translation="MAKKNLVIVESPAKAKTIKKFLGKNYDVEASMGHVVDLPKSKMG
                     INIEENFEPKYITIRGKGKVLKKLRKAKKKSENVYLATDPDREGEAISWHLSRALKLE
                     ESKNRIEFNEITKNAISNAMDNPREIDQDLVDAQQARRLLDRLVGYKLSPLLWKKVRK
                     GLSAGRVQSVAVKIICQREEEIENFEPVEYWSITAVFNDGQENFEADLFKIEGEDFDL
                     GSEKEVNKVLKDLEKNKYVIDNIKIKKRRRNPYPPFTTSTLQQRASSVLNFSAKKTMF
                     IAQQLYEGIEIGSEGTAGLISYIRTDSTRLSNEAKGEAKNYIIDNFGDKYYQGKSYGK
                     SGSNTQDAHEAIRPTSAFRSPDKIKKYLSKDQYALYNLIWNQFIGSQMKPAVYKTYAI
                     DILNGKYNFRFTGSELIFPGFLRLFNGDNKDDYIPEVEEKDEIEASEIEPEQHFTNPP
                     PRYTEASLVKTMEEEGIGRPSTYAPTLSKIVSRNYVDKKGKYFIPTDLGKTVNKLLNE
                     YFPDVTDIEFTAQLEKRLDQIEEGKEEWQQILKDFYSDFKERLDEARENMEKVNMDIE
                     TDIKCEKCGRPMVIKHGRYGKFLACSGFPECKNTKPYLDKIGVDCPKCEDGEIVKRKS
                     KKGKTFYGCSNYPDCDFVSWDKPTNKKCPKCGSMMVEKYSKKSGKYYKCTNKECKHTV
                     KE"
     gene            3872..4408
                     /gene="hslV"
                     /locus_tag="VKN64_10235"
     CDS             3872..4408
                     /gene="hslV"
                     /locus_tag="VKN64_10235"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HMA60662.1"
                     /translation="MEKIKATTIVAVKYKDKVALAGDGQVTMGDTVMKATARKVRRLY
                     NDEILAGFAGTSADAFTLFEKFEGKLEEYHGNLERAAVELAKEWRTDKMLRRLEALLL
                     VADKEKLLLISGTGDVIEPDDEILAIGSGGAYAKAAARALIDSNQDLSAKDIATRALK
                     IAADICIYTNDNIITEDL"
     gene            4423..5808
                     /gene="hslU"
                     /locus_tag="VKN64_10240"
     CDS             4423..5808
                     /gene="hslU"
                     /locus_tag="VKN64_10240"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HMA60663.1"
                     /translation="MFMELTPSQIVEKLNQYIVGQNDAKKSVAIALRNRYRRKLVDKD
                     LQEEIIPKNILMIGPTGVGKTEIARRLANIAEAPFIKVEVTKFTEVGYVGRDVESMVR
                     DLIESAIRMIKEKKMEEVEKEALRAARERIIENILPMPSQKKKGPFQFLSDEEEKEEE
                     QSESYQRIQRRRDKLMEKLKEGELDDRKIEISIEKDSPQMMELFTGQGVEEMGINFQD
                     MFGDIFPKQKKTRKVKIKEAIDILKDEEAKKLIDNEEVKSEAINLVEDEGIIFLDEID
                     KVAGKDQASGPDVSREGVQRDILPIVEGSTIMTKHGSVKTDHILFIAAGAFHMSKPSD
                     LIPELQGRFPLRVELKSLSVDDFEQILLQPRNALTKQYRALLETEGMNITFTDDAIRE
                     IAEFAYEVNEQTENIGARRLHTIMEKLLEKLSFNAPDNIENNEVTIDVDYVNEQLSEV
                     VKDKDLSKYIL"
     gene            5841..6623
                     /gene="codY"
                     /locus_tag="VKN64_10245"
     CDS             5841..6623
                     /gene="codY"
                     /locus_tag="VKN64_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HMA60664.1"
                     /translation="MTDLLEKSRKINRLLQRSGGQPVDFEKMAPVLSEAIECNVYIAS
                     KKGKILGHAFMEDFECEIMDEIVSEDNRFPEDYNESLLQVNGTRANVTQEDQVCVFDP
                     EQDCMYSDKKTTIVPVIGGGDRLGTLVLSRYGEEFSDTDLVLAEYGASVVGMEILRKR
                     SNEIEKEARKRAAVQIAIDTLSYSELEAIEHIFDELDGEEGLLVASKVADRVGITRSV
                     IVNALRKFESAGVIESRSLGMKGTYIKILNDYLLEDLEKLKM"
     gene            6846..7598
                     /gene="rpsB"
                     /locus_tag="VKN64_10250"
     CDS             6846..7598
                     /gene="rpsB"
                     /locus_tag="VKN64_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMA60665.1"
                     /translation="MSVVNMKQLLEAGVHFGHQTRRWNPKMKDYIFTERNGIYIIDLQ
                     KTVRLMDRAYEFVKEEAAKGKTILFVGTKRQAQETIKKEAERCDMPYVNQRWLGGMLT
                     NYQTISKRIDRLNELEQMEEDGLFEVLPNKEVIELERERDKLRKNLGGIRDMGGLPDV
                     VYITDPRKESIAVSEARKLDIPIVSILDTNCDPDLIDYEIPGNDDAIRAVKLISNKIA
                     DAVLAGKQGEQLNEEQVQGEELNDDLEVVEEQ"
     gene            7672..8574
                     /gene="tsf"
                     /locus_tag="VKN64_10255"
     CDS             7672..8574
                     /gene="tsf"
                     /locus_tag="VKN64_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMA60666.1"
                     /translation="MSKSMKDIKELRERTGAGVLDCKKALDETDGDIEAAIDFLREKG
                     MSEAAKKSGRVAAEGIVDVLINEKRDKGIIVEVNSETDFVAKNERFQDLVSSISEHIM
                     LSNAENLEQLNAEKWFKDESKTVETIIKEAIAGIGENINLRRYEKIETDGFLYGYIHL
                     NGKIGVLAEFEGEKTEEALNTAKNISMHIAASNPEFLSREDVDEEVVEKEKAIIKEQL
                     KNEGKPDHILDQIVEGKINKFYTQICLLEQEYIRDTDLQVQDVLDESDVAVKQFVRYE
                     LGEGIEKKQEDFAEEVMAEVNKNE"
     gene            8682..9401
                     /gene="pyrH"
                     /locus_tag="VKN64_10260"
     CDS             8682..9401
                     /gene="pyrH"
                     /locus_tag="VKN64_10260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMA60667.1"
                     /translation="MTRTPKYSRVLLKLSGAALSGDREYGIDPLTIQSVAGKINEIVS
                     DTDVEIAIVVGGGNIFRGVAGSAKGMDRGTADYMGMLATVINALALQDAIEKYGLETR
                     VQSAIEMRQIAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTTAALRAAEISAEAI
                     LMAKNVDGVYDDDPLKNDNAVKYKELTYIEVLNKSLKVMDSTAISLCMDNNIPLIVFG
                     LDDYDNIEKAIIGENVGTVVK"
     gene            9428..9985
                     /gene="frr"
                     /locus_tag="VKN64_10265"
     CDS             9428..9985
                     /gene="frr"
                     /locus_tag="VKN64_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMA60668.1"
                     /translation="MIGQITKKAEKKMEKSFQNTKEDLNTVRTGRATPSLVENIIVDY
                     YGSSTPLQQMAKIVAPEPRQLVIEPWDKNVIENIEKAIMKENLGLTPNNDGNVIRINI
                     PKLTEERRKELVKILHEKAEKGKVNIRNIRHNIKEELEDLEDEGEISEDNYYRALDNL
                     QELTDDYTKKIEDYVDKKEEIIMEV"
BASE COUNT         3843 a         1415 c         2129 g         2745 t
ORIGIN      
        1 taatccggtt aataatgtaa ataatttaag taaagatgcg gtcatggtaa tatcagaccg
       61 cggttactta aaagtagaaa gagcctggaa aaaggaaata gctgaaaaga ttaaatgccg
      121 ctttgtactt gttaatacca atacaattgt tccggttgag attgcttccg acaaagagga
      181 atatgcagcc tatacgatta gaaagaagat caataataaa gtagaaagat ttatagatgt
      241 ggtagacaag gagaaattaa aagtaaattc tcttcgatta aatattaaag gagccgattt
      301 aaatgatatt gataacctag taaacaggtt atcgatagat aaaactgtta aaccgagtcc
      361 tgcctttaaa ggtggttcta gcgaggcttt aaacaggttg gaaagattta tgggaaaaaa
      421 cctaaaatta tatgaagagc acggaagtaa tcccgactat gatgtaactt ctaaattaag
      481 tccctattta cattttgggc agatctcgcc tgcctatatc tataagagaa taaaatggaa
      541 tgatattaag gcggaagact tcttagaaca gttaatagtg cgaagagaac ttagttttaa
      601 ttatatatat tataatgata actataacca aaagttaaca gacatcttac cggactgggc
      661 ggttgaaaca ttagaagaac atgagagtga taaacgagag tatatctaca gtagagaaca
      721 attagaaaat tatcaaacac atgatcaata ctggaatgca gcccaaaaag agatggtatt
      781 aagcggctat atgaatggat atatgcggat gtactggggt aaaaagatcc tggaatggac
      841 ccaggatccc caaaaggctt ttgatataat actatatcta aataataaat attttttaga
      901 tggaagagat cccaatggtt atgccggtgc agcctggata ttcggaaagc atgaccgggc
      961 ctggcaggag aggcagattt ttggtaaggt aaggtatatg aacaagaacg gacttgagag
     1021 aaagttcgac atggaagatt acctaaaaaa agtaaataat ctaaactgat ttttataaga
     1081 ataaaacaaa tattagctct ttatccagga aaaaaacttt acaaacaatt ttaaaacatc
     1141 tataatatta taatagttat atacaagagt aaatcaaacc aaggaggtat tagcaggttt
     1201 tacctgcaga tatatatgag agaaaagata tttgatattt taagttatct tatagaggaa
     1261 cttttaaata gtggagatga agttgttgac caggaagact tgatcaatga attggtcgac
     1321 aagggatatg aaattgaaga tattgatgaa gcttttcaat taatttttga aaagccggaa
     1381 atcattgagc acagtgatat agaaggtatt tcagaagaca atttccttaa tagggtcttt
     1441 actgtacagg agaaaatgta ccttcctaaa gaacaccgcg gtttaataaa aagactgcta
     1501 atcatgaaca ttattaccat taaagaaaca gaagaaatag taaataagat catccagaat
     1561 gtctattata acaataacaa gaactttgat ttctgggaat taattcaaga tgtagtagat
     1621 gatgaaaacc gtatcaatta tatttcaaac cattttaagg aattttcaac aaaattatca
     1681 aaagatttta aatacattaa ttaaatattt tggaggttaa tttgatcatg gctaaaaaga
     1741 acttagttat cgtagagtcc ccagcaaaag ccaagacaat caaaaagttc ttaggcaaaa
     1801 attatgatgt agaagcatcg atgggacatg tagttgattt acccaaaagt aaaatgggta
     1861 ttaatataga agaaaatttt gaacctaaat atattaccat tagaggtaaa ggtaaggttt
     1921 taaaaaaatt aagaaaagca aaaaagaaaa gtgagaatgt atatcttgcg actgaccctg
     1981 acagagaagg ggaagcgatc tcatggcact tatccagagc tcttaaatta gaagaaagca
     2041 aaaataggat tgaatttaat gaaataacta aaaatgcaat ttctaatgct atggacaatc
     2101 caagagagat tgaccaagat cttgtcgacg cacaacaggc cagacgcctc ttggatagat
     2161 tggttggtta taaattaagt cccttactct ggaaaaaagt aaggaaaggc ttaagtgccg
     2221 gccgtgttca atcggttgcg gtaaaaataa tctgtcaacg ggaagaagag attgaaaact
     2281 ttgaaccggt agaatactgg tctattacag ctgtctttaa tgatggccaa gaaaattttg
     2341 aagccgatct atttaagata gagggcgaag actttgatct aggtagtgaa aaagaagtaa
     2401 ataaggtatt aaaggattta gaaaaaaata aatatgtgat cgataatata aaaattaaga
     2461 aaagaagaag gaatccctat ccacccttta caaccagtac cttacagcag agggcttctt
     2521 ctgtacttaa tttttctgct aaaaagacta tgtttatcgc ccaacagtta tatgaaggta
     2581 tagaaatcgg tagtgaaggt acagccggtc tcataagtta tattaggact gatagtacca
     2641 gattatccaa tgaagcaaaa ggggaagcca aaaattatat aattgataac tttggagata
     2701 aatactacca gggcaaatcc tatggtaaga gcggcagtaa tactcaggac gcccatgagg
     2761 caattagacc aacatctgcc tttaggtcgc ccgataaaat caaaaagtat ttatcaaaag
     2821 accagtatgc actatataat ttaatctgga atcaatttat cggcagtcag atgaaaccgg
     2881 ctgtctataa aacctatgcc attgatatct taaatggaaa gtataacttc aggtttacag
     2941 gttctgagtt aatctttccc ggttttttaa gactatttaa cggagataat aaagatgatt
     3001 atatacctga ggtggaagag aaagatgaaa tagaagcatc cgagatagaa cctgaacagc
     3061 actttacaaa tccaccaccg cgctatacag aggcttcttt agtcaaaaca atggaagaag
     3121 aaggtattgg taggccgagt acttatgctc ctaccttatc aaaaattgtt tcacgaaatt
     3181 atgttgacaa aaaaggtaag tactttatac cgactgacct cggtaaaacg gtgaataaat
     3241 tattaaacga atattttccc gacgttactg atatagaatt tactgcccaa ttagaaaaaa
     3301 gattagatca aatcgaagaa ggtaaagaag agtggcagca gatcttaaag gacttctatt
     3361 ccgattttaa agaaagattg gatgaagcaa gggaaaatat ggaaaaagtt aatatggata
     3421 tagagactga tattaagtgt gagaaatgtg gtagaccgat ggtaattaaa cacggtagat
     3481 acggtaaatt tctagcttgc tctggttttc cagaatgtaa aaatacaaag ccttatcttg
     3541 acaaaatagg tgtagattgt cctaaatgtg aagatgggga aatagttaag agaaaatcca
     3601 aaaaaggtaa aaccttttac ggttgtagta attatcccga ctgtgatttc gtttcctggg
     3661 ataaaccaac caacaaaaaa tgcccaaaat gtggtagtat gatggtcgag aaatacagta
     3721 aaaaaagtgg taaatactat aaatgtacta ataaggaatg taaacataca gttaaagaat
     3781 aattgaatat ttgacacagt gaaattgctt gtgttaaact aaatccatca gaagatttaa
     3841 aattttgcaa atattatagg aggtaccatt aatggagaaa ataaaagcaa cgacaatagt
     3901 tgctgttaaa tataaggata aagtagcttt agccggagac ggccaggtaa caatgggaga
     3961 tacagttatg aaagcaactg cccgcaaggt cagaaggtta tataatgatg aaatactggc
     4021 gggctttgcc ggtacttctg ccgatgcatt taccctattt gaaaaatttg aaggaaaatt
     4081 agaagaatac cacggtaact tagaacgtgc tgcagtagag ttagccaaag aatggcggac
     4141 tgataaaatg ctaagaaggt tagaagcatt gttattagtt gctgataaag aaaaactatt
     4201 attaatctcc gggaccggag atgtaataga acctgatgat gaaatattgg caattggttc
     4261 cggtggcgct tatgctaagg ccgctgccag agccttaatt gacagcaatc aggatttatc
     4321 agccaaagat atcgccaccc gggctcttaa aattgcagcc gatatttgta tttatacaaa
     4381 tgataatata attaccgaag atttataata tatattagga ggatgtttat ggagttaaca
     4441 ccgagtcaaa tagtcgaaaa acttaatcaa tatatagtag gacagaatga tgcaaaaaaa
     4501 tctgtcgcta ttgccttacg aaatagatat agaaggaaat tggtagataa agatttacag
     4561 gaagaaatta taccaaagaa tatcctgatg atcggcccaa ccggtgtagg taagacagaa
     4621 atagccaggc gtttagccaa tatagcggaa gcacctttca tcaaggtaga agttactaaa
     4681 tttactgaag taggttatgt aggccgagat gtagaatcaa tggttagaga tttaatagaa
     4741 agtgctatta gaatgatcaa ggaaaagaag atggaagagg tcgaaaaaga agccctaagg
     4801 gcagcccggg aaagaataat tgaaaacata ctcccgatgc ccagccaaaa gaaaaaaggt
     4861 ccctttcagt ttctatcaga tgaagaagag aaagaagaag aacaatctga aagctatcag
     4921 agaatccagc gccgtagaga taaattaatg gaaaaattaa aggaaggcga gttagatgat
     4981 agaaagattg aaatatcaat tgaaaaggat tctccccaga tgatggagct ttttaccggc
     5041 cagggtgtag aagaaatggg tataaatttc caggacatgt ttggagatat ttttcccaaa
     5101 caaaaaaaga ccaggaaagt taaaataaaa gaagctattg atattttaaa agatgaagag
     5161 gcaaaaaaat taatagataa tgaagaagtt aaatcagaag ccatcaacct tgttgaagat
     5221 gaaggtatca tctttttaga tgaaatcgat aaagttgccg gtaaggatca ggcttctgga
     5281 ccggatgttt cgagagaagg agtccaaaga gatattcttc cgattgtaga aggatctacg
     5341 attatgacta aacacggttc tgtaaaaaca gatcatattc tctttatcgc agcaggagct
     5401 tttcatatgt ctaaaccgtc agatttaatt cccgaactac agggtagatt ccctttaagg
     5461 gtagagctta aaagtctttc agttgatgac ttcgaacaga ttctacttca accgagaaat
     5521 gccctaacca aacagtatcg ggcccttttg gaaacggaag gtatgaatat tacatttact
     5581 gatgatgcta tccgtgaaat tgcagaattt gcctatgaag ttaatgaaca gaccgagaat
     5641 attggtgcaa gaagacttca tactataatg gaaaaactat tagagaaact ttcatttaat
     5701 gctccggata atatagaaaa taatgaagta acaatagatg ttgattatgt aaatgaacaa
     5761 ctttccgaag tagtgaaaga taaagacttg agcaaatata tcctataaat tataaaatta
     5821 aataaatgga ggtattttaa atgactgatt tattagaaaa aagtaggaag attaatcgcc
     5881 tattacagag atcaggcggc caacctgttg attttgaaaa gatggccccg gttctctccg
     5941 aagcgattga atgcaatgtt tacattgcaa gtaaaaaagg taaaatatta ggacatgctt
     6001 ttatggaaga cttcgaatgt gagataatgg atgaaatagt tagtgaagac aatagatttc
     6061 ctgaagacta taatgagagt cttttacagg taaatggtac cagggcaaat gtaacccagg
     6121 aagaccaggt ttgtgtattc gatccagaac aagattgtat gtattccgat aaaaaaacta
     6181 ctatcgtacc ggttattggg ggaggagacc gtttaggtac attggttctt tcccgttatg
     6241 gagaagaatt tagtgatact gatttagtat tagcagaata cggtgcatct gtggtaggaa
     6301 tggaaattct cagaaaaaga agtaatgaaa tagaaaaaga agcccgtaaa agagcagcag
     6361 tgcaaattgc aatcgatacc ctatcatact cagaactgga agctatcgag catattttcg
     6421 atgaactcga tggtgaagaa ggtttattag tagccagtaa ggttgctgat cgggtaggaa
     6481 ttacaagatc ggtaatagtt aatgccttac gtaagtttga gagtgctggt gtaatcgaat
     6541 cccgttctct cggtatgaag ggtacttata taaaaatctt aaatgactac ctacttgaag
     6601 atttagaaaa gttaaaaatg taattttacc caaatatgaa taaatttaat attttccaaa
     6661 aatattaatt gattatacca cttttatgtg ttatactagc taatggtaat aaataaacac
     6721 atcgcgtgga ccctgtcact ggtgctgttt tagagacagt agtggtatcg aggtttaatc
     6781 ctcttcgata tatttgcagg ggaagaagcg gatggaagtt taaaaccaga gaggaggtgc
     6841 caatattgtc tgtagttaat atgaaacaat tactggaagc tggtgtccat tttggtcacc
     6901 aaacaagaag atggaaccct aaaatgaagg attacatctt tacagaaaga aacggtatct
     6961 atatcatcga tttacagaaa actgtaagat tgatggatag agcttatgaa ttcgttaaag
     7021 aagaggctgc caagggcaaa acaatccttt ttgtgggaac taagcgacaa gctcaagaaa
     7081 ccattaaaaa ggaagctgaa cgctgtgata tgccttatgt aaaccaacgt tggttaggtg
     7141 gtatgttaac gaactaccaa accataagta agcgcataga ccgtttaaat gaacttgaac
     7201 agatggaaga agatggtcta ttcgaggtct taccgaacaa agaggttatt gagttagagc
     7261 gtgaaagaga caaattgcgc aaaaacctgg gtggtattcg tgatatgggt ggattacccg
     7321 atgttgttta tatcactgac cccagaaaag agtcaattgc tgtgtctgaa gccagaaaac
     7381 ttgatatccc aatcgtttct attttagata ctaattgtga tcctgatcta atagattacg
     7441 aaattcccgg taatgatgac gccattagag cggtaaaatt aataagtaat aaaattgctg
     7501 atgctgtatt agcaggcaaa cagggagaac aacttaacga agaacaagtt cagggtgaag
     7561 aattaaatga tgatcttgaa gttgtagaag aacaatagaa cttacttaag gcgttgatta
     7621 aagggctccc tttttcaacg tcttttttta taaaacaaag gagttgataa aatgtctaaa
     7681 tcgatgaaag atatcaagga attgcgagaa agaaccggtg ctggtgtact ggactgtaaa
     7741 aaagcattag acgaaacaga tggcgatatt gaagctgcta tagatttctt aagagaaaaa
     7801 ggtatgtctg aggccgctaa aaagtcgggc cgtgtggcag ctgaaggtat agttgacgta
     7861 ttaattaatg aaaaaagaga taaaggtata attgtagagg ttaacagcga aactgacttt
     7921 gtagcaaaaa atgaaaggtt tcaagatctc gtatcaagta tctcagaaca catcatgtta
     7981 agtaatgctg aaaatttaga acagttaaat gccgaaaaat ggtttaaaga tgaaagcaaa
     8041 actgtagaga ctatcatcaa ggaagctatt gcaggaattg gtgagaatat caatcttaga
     8101 agatatgaaa agattgaaac tgatggattt ctctatggtt atatccattt aaatggtaaa
     8161 attggagttt tagctgaatt tgaaggcgaa aaaacggaag aagctttaaa tacagctaaa
     8221 aatattagta tgcatattgc agctagtaat ccagaattct taagcagaga agatgtagat
     8281 gaagaggttg tagaaaaaga aaaggcaatt atcaaagaac agcttaaaaa tgaaggaaaa
     8341 cccgaccata tactagatca aattgtagag ggtaaaatca ataaatttta tacacaaatc
     8401 tgtctattag aacaggaata tatccgtgat acagacttac aggttcagga tgttttagat
     8461 gaatctgatg ttgctgtaaa acagtttgtc cgttatgaac tgggtgaagg aattgaaaag
     8521 aaacaagaag actttgctga agaagttatg gcagaagtta ataaaaatga gtaattgatg
     8581 ttattaaaaa gagagcaccc tgggtgttct ctttttcaat gtaaaataca aataaaggat
     8641 ttttatatgg aatcgagaat atatttcttg gaggtagagt aatgactcga acacccaaat
     8701 attccagagt tttgctaaaa ttaagcggtg ctgccttgtc tggtgatagg gaatatggga
     8761 ttgatccttt aactattcaa agtgtagccg gcaaaattaa tgaaatagtc agtgataccg
     8821 atgtagagat tgctatcgta gttggtggcg gcaatatttt tagaggtgta gccggatctg
     8881 cgaaaggaat ggaccgtggt acagctgatt atatgggtat gctggcgacc gtaatcaatg
     8941 ctttagcctt acaggatgcc attgaaaagt acggcctgga gactagagtt caatctgcta
     9001 ttgagatgag acagatcgct gaaccctata ttagaagaag ggccatccgt catcttgaaa
     9061 agggcagagt ggtcattttt gcggccggta ccggtaatcc cttttttaca acagatacga
     9121 cagctgcgct tagggcagca gaaatttctg ctgaagcaat tctcatggcc aaaaatgttg
     9181 atggagtcta tgatgacgat ccgctcaaaa atgataatgc agtaaaatat aaagaactaa
     9241 cctatatcga agtactaaac aaatcattga aagtaatgga ttcaacagct atttcacttt
     9301 gtatggataa caacatacct ttaattgttt tcggtcttga tgattatgac aatattgaaa
     9361 aagcaattat tggggaaaat gtgggaactg ttgtaaaata aatctattac attaaggggg
     9421 aagttaaatg attggtcaaa taaccaaaaa agctgaaaaa aagatggaga agtcttttca
     9481 aaacaccaaa gaggatttaa atactgttag gacagggaga gcaacacctt ctctagttga
     9541 gaatattatt gttgattatt atggttcaag tacacctctc caacagatgg ctaaaatagt
     9601 agctcctgaa cccagacagt tagttattga accctgggat aagaatgtta ttgaaaacat
     9661 tgaaaaagcc attatgaaag aaaatcttgg tttaactcca aataatgatg gtaatgtaat
     9721 cagaatcaat attcccaaac tgacagaaga aagaagaaaa gaactggtca aaatattaca
     9781 tgaaaaggct gaaaaaggta aagttaatat tagaaatatt agacataata taaaagaaga
     9841 gctggaagat ttagaagatg aaggtgaaat ttcagaagat aactattacc gggctctgga
     9901 taatctacaa gaacttactg atgattacac aaaaaaaatt gaagattatg tagataaaaa
     9961 agaagaaata atcatggaag tataatgatt aaacaagata aatttctctt agaaacacca
    10021 gcagaatata tcaatagcct tccatcagat aagaactatg agttgaaaga atatcagccg
    10081 ccacatgata aagttggcag agggaataag attattttaa aaattattag aa
//
LOCUS       DATGCJ010000232        12653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_303563, whole genome shotgun sequence.
ACCESSION   DATGCJ010000232 DATGCJ010000000
VERSION     DATGCJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12653
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303563"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..983)
                     /locus_tag="VKN64_10270"
     CDS             complement(303..983)
                     /locus_tag="VKN64_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60669.1"
                     /translation="MKYTLLIIGITIVFIFSFTLITAAHKPVNVDFPATKQSPILIED
                     HQISWAAYTELKKINEVDYYKFEAKADEEIYANLLIPDIERLKDFHPTFALIGPGLPK
                     EYEVENVTLDIDEEEGVIVKRYRAETLEIFFEPFTQTSYYVKQTLTKKAPTDGTYYLA
                     VFDENKEKGKYVFAIGRKEKWKANEIIKLPKTWWDTRMFMEKEKSTYAITIGIGVVGL
                     YLLSTLFK"
     gene            complement(1084..2265)
                     /locus_tag="VKN64_10275"
     CDS             complement(1084..2265)
                     /locus_tag="VKN64_10275"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HMA60670.1"
                     /translation="MNKDFQDFMDKELEQLKEEGLYNTIRTLEGPQGAWVEMEGKEVL
                     NFSSNNYLGLANHPETVQAAKKALMNYGVGPGAVRTIAGTMELHNKLEEKLAEFKKVE
                     AALTFQSGYNSNLAVIPAIVGKGDVIYSDELNHASIIDACRLSGAKIKVYKHGDMESL
                     ESILQEEREGKGLIVTDGVFSMDGDVAKLPEIVKLAEKYNAITMVDDAHGEGVLGTHG
                     RGIVDHFNLHNEVDIEVGTFSKAIGTVGGCAAGSAKMVEYLKQKARPFLFSSAVTPPD
                     VAATLKSIEILEEDDSLVKKLWDNGDYFKEKMQEIGFDVGNSQTPITPVMLGKAKLAS
                     EFSKKLFEESVFAQSIGFPTVPKGKARIRVMISATHSRDDLDFALDKFKKVGQELNII
                     K"
     gene            complement(2279..3319)
                     /gene="tdh"
                     /locus_tag="VKN64_10280"
     CDS             complement(2279..3319)
                     /gene="tdh"
                     /locus_tag="VKN64_10280"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HMA60671.1"
                     /translation="MKGKMHAVVKEKPEYGAVYKEVDIPEVGEEQVLIKVESTSICGT
                     DHHIYIWNKWAQENINPPQIMGHEVAGEVVEVGSRVEKVKKGDFVSSETHIPCGHCYQ
                     CQTGQQHICQNMKIFGVHQDGVFAEYAVLEEVDVWKNDPSIPKDFASIQEPLGNAIDT
                     ILPGNVAGKDVLITGAGPVGLISIAVAKTFGAANILVSEPNEYRRDLSFEMGADHVIN
                     PIETDLLEAVDDIFGKKGVDFTAEMSGHPDAIRDGIKAVTGGGIVSLLGLPDDKVSLD
                     LTNDIIFKNIKVVGVTGRKMFSTWHIASNLLKGERIDLSPVITHKFPLEDFEKGMKLM
                     DEGNCGKIILKP"
     gene            3511..4035
                     /locus_tag="VKN64_10285"
     CDS             3511..4035
                     /locus_tag="VKN64_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HMA60672.1"
                     /translation="MDNTFKTKYLILTGLFAALTAIGAYIYIPLPISPVPITLQMFFT
                     FMAGGLLGKKYGFFSQLIYILIGAIGLPVFAGGTGGFGILFGPTGGYVLGFLAAGYIA
                     GFSKDTFISKLLYMLLALIVIYLLGTTRLMMVIQVDFIKALTIGVTPFIIGDLMKVAL
                     AAYLSVKLSNYIRY"
     gene            complement(4016..4948)
                     /locus_tag="VKN64_10290"
     CDS             complement(4016..4948)
                     /locus_tag="VKN64_10290"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HMA60673.1"
                     /translation="MSSQNKNRSAYIIGLILIGIGLLFLLNTMGIATFQVWYFLFKFW
                     PVILILIGLNIILKKTALWWIVPILIFVIFIGAIVIPDSMLRRLDSPRLYFNFGDREY
                     SGSYQEDREFDSSIEKLDVEFRYGANRLNVGKVSNDDFLYDINLDYRGESPKIYYNRA
                     DSRAELEIEQAKKVNIGTDGSETWNVSLTDRIPIDMQVKSGAGDLWMDLEELKVANLD
                     IDTGLGQLRIRYPDYDNETEISAGAGNIKLIVPAQTALRVETSTVINNNNFEESGLIQ
                     LYEDVYQSKNYGEADNRIKIKISTPASNINIEYN"
     gene            complement(4945..5400)
                     /locus_tag="VKN64_10295"
     CDS             complement(4945..5400)
                     /locus_tag="VKN64_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMA60674.1"
                     /translation="MDKKLYRSKKDQIIGGVCGGIAEYFGIDSTLVRLAFVLFALIEG
                     AGIIAYIIAWIIIPERPEGTQKSEEEVYDVEIDKEDEEGEYKASAQEPEEEKKSKEQR
                     KRILGLILVALGIFFIIDNWIPGFYWRRFWPVILIVLGVVLIYREVRGK"
     gene            complement(5459..6319)
                     /locus_tag="VKN64_10300"
     CDS             complement(5459..6319)
                     /locus_tag="VKN64_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMA60675.1"
                     /translation="MDRYSQGKKISYITISGNIFLALLKIIIGTLVASSALIADGFHS
                     VSDVASTLAVLIAIYVSSKPPDEEHQYGHGQAESIAAKILGILLLLTGLALGYNILRQ
                     ILNQNFGVPGIYGFWAAVVSIIVKELMFRYTHKVGEETNNQALIADAWHHRSDAISSI
                     AAAVGILGANMGYPILDPIAGIIVAILIIRVGWNIIVDAVNTLMVKAPPKEDSEDIRK
                     TVNDIEGVKEIKELRAHYSGVDLYVDLRIVVDGNLTVKQGHDISVEVRNTLLKECEQV
                     KEVIVHIHPG"
     gene            6413..7168
                     /locus_tag="VKN64_10305"
     CDS             6413..7168
                     /locus_tag="VKN64_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HMA60676.1"
                     /translation="MSITTIVLLCMVGFAAAFLDSIAGGGGILTVPALMAAGLPPHLT
                     LGTNKFSSTNASFTSTLTFLNSKKIHIPLIKYILPFSFLGAVLGVRTVLQINEEFLRI
                     IILVLLIFVALNTLFNKNVGTENNFQGVNKKTIVIGCIVGFTIGFYDGFFGPGTGSIL
                     LYAFIRFFKFDFTIGTANARILNFTSNITSLVLFAYYGRIVYAVGIPMALSMILGAFL
                     GTRMAIKHGGKIIKPIFVTMSLAIVVKLFLDTI"
     gene            complement(7169..8911)
                     /locus_tag="VKN64_10310"
     CDS             complement(7169..8911)
                     /locus_tag="VKN64_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60677.1"
                     /translation="MIRKFSKYYINHWKLFLLDIVCAFLMSSIDLLYPVIVQRLLDNY
                     FPNKNASMIIRMLFILLILYVLRLIFQYIVHYWGHVVGIRMETDMRKELFSHLQKLSF
                     KFYDNTKVGYLMSRIVNDLNNVSELAHHGPEDLFISTVMILGSFTILLFMKWQLALFT
                     FALLPIMFYFSLILGKKMYRSFKDIREKVAEVNSLVEDSLSGIRVVKSFTNEKLEEDK
                     FDDGNQEYRQSREVAMRNMAQFHSGMNFFINLIRFFTLGAGAYFLFTDTLTTGELVAF
                     LFYVNMFMNPIRRLVNFNQQFQKGMAGFNRFLELLAIKPEIEDGSDAVKLEDIEGKLK
                     YDDVTFGYDNHSKVLKNINLDISPGETVAFVGPSGAGKTTLCNLLPRFYEIDSGEITI
                     DGTSIQDIKLESLRENIGIVQQEVFLFNGNIKENISYGNFNASEEQIKEAAVKANAHQ
                     FITNLSNGYETKIGERGVKLSGGQKQRISIARSFLKNPPILILDEATSSLDNESEKII
                     QASLDRLSKDRTTLVIAHRLSTVKNADKIIVLTEDGIVEKGTHQDLINKEGGVYNRLY
                     KTQFNEADEDIYFD"
     gene            9085..10110
                     /locus_tag="VKN64_10315"
     CDS             9085..10110
                     /locus_tag="VKN64_10315"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HMA60678.1"
                     /translation="MELYPLKFFPIYKNKIWGGNRLKSKLNRDIKEENVGESWEISTN
                     KDSISIVKNGKHENKKLTELIQEFPKELLGENYSSNDKFPLLLKIIDASKKLSVQVHP
                     DDKMAQELANGNGKTEMWYVLDANKEAKLYIGLKDIKGKNHLREVIEKGQLQNYLNTI
                     NVQRGDFFYIPAGTVHAIGGGLMLVEIQQNSNTTFRLYDWNRVDENGESRELHLEDGI
                     KATNLKADVERYESIYKKETPGYKRKVLTISNYFVSEKVTVKNSFQLDHDNFALITCI
                     ENNGLIKYGKNKVEPLKEGESVMIPAGLEDVEIEGKMEFLYMYIPKDVNYCIQRLKSK
                     GFTSFNL"
     gene            complement(10220..11092)
                     /gene="pstA"
                     /locus_tag="VKN64_10320"
     CDS             complement(10220..11092)
                     /gene="pstA"
                     /locus_tag="VKN64_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMA60679.1"
                     /translation="MRDTDINLEKRHSRQKIVFSILGITLLLVLFILGIIIYFVFMRG
                     IGVIDWEFLTAMPRNGMTEGGIFPAIVGTFYLVIGAILFATPLGVFAAIYMTEYADKG
                     KIITAIRIGVNNLAGVPSVVFGLFGLGIFVKFLGFGVSILSGSLTLAIVILPTIIRAS
                     EEALMAVPDEYRHASFAMGATNWQTIKNVVLPSAMPGILTGVILGIGRVAGETAPIIF
                     TAATYFQMRLPDSVFDEVMALPYNIYALVTAGTFPSKQVPIAYGSAVVLLSLVLLINL
                     VAIILRLKFSRNQE"
     gene            complement(11089..11973)
                     /gene="pstC"
                     /locus_tag="VKN64_10325"
     CDS             complement(11089..11973)
                     /gene="pstC"
                     /locus_tag="VKN64_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HMA60680.1"
                     /translation="MDWRKKEKIIKYILLFFALSSIIFLTGIVFVLFREGLPIFQEVG
                     VFDFIFGEDWYPTYDPPAYGILPLLSASLWVTFGSMVLAVPIGLASSVYISYVLPKNL
                     KKFVKPAIELLAGIPSVIYGLFGMKILGPFLKDVFGLPTGLTALNASIMLGIMALPTI
                     VSLSEDAITSIPKRYRDASLALGGTRWETISKVILPTASSGIVTAVILGMGRAIGETM
                     TVLMVAGGAAAMPSSFLKPVRPMTATIAAEMGEAPVGSEHYQALFGIAIILFIITLTF
                     NIVADLASRRFKKKVSGQ"
     gene            complement(12074..>12653)
                     /locus_tag="VKN64_10330"
     CDS             complement(12074..>12653)
                     /locus_tag="VKN64_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMA60681.1"
                     /translation="SRFIKQSEVENAVNNGYYPVPHRVAMDGIAVVINPANPVESLTL
                     DEIKQIYTGEITNWSELGGNDEEIVVISRDSSSGTFEVFGEIALQGERLTPNALLQAS
                     NGAISSVVSDTEGAIGYVGLGYLDDTLKAVKVNGVAPSNSTVASGEFPIARPLFMFTD
                     GWPEGLTLRFINFVLSQEGQKVVEEQGFVPLF"
BASE COUNT         3996 a         2504 c         1939 g         4214 t
ORIGIN      
        1 gcctcttttt ctttataacc ggtctcaggg ttattatata tttgttggac tatatcaata
       61 atttcatttt tattttcttc tattttcttg caaaccttat ttttcaactt atcccgtctc
      121 atatttatac ctcttcccaa aagaaaaaga agggatcttt cccttctcta ttgttttttt
      181 attcttctta aattaaaaaa tgctgttaat ttattatatc aactcaaagt aatattttca
      241 tatataatca aaataaattt gctgaaatct tattataaat ttagattaat aatagaaaaa
      301 tactatttga ataacgtact aagtagatat aaccctacaa ctccaattcc tatcgtgata
      361 gcatatgttg acttttcttt ttccataaac atcctggtat cccaccaggt cttaggaagt
      421 ttaattattt cattggcttt ccatttttct ttacggccaa ttgcaaaaac atatttgcct
      481 ttttccttat tttcatcgaa aactgctaga taataggtgc cgtcagtcgg ggctttttta
      541 gttaaggttt gtttcacata atagctggtt tgggtaaaag gctcaaaaaa tatttcgagt
      601 gtttctgctc tatacctttt tacgataact ccttcctctt catcaatatc aagtgtaaca
      661 ttttccactt catattcttt tggtaaaccc ggccctatta aggcaaaggt gggatgaaaa
      721 tcttttaacc tttctatatc gggtattaat aaattagcat aaatctcttc atcggctttc
      781 gcctcaaatt tataatagtc tacttcatta atttttttta gttccgtata ggctgcccat
      841 gatatttggt ggtcctctat taatatgggg gattgttttg tggccggaaa atctacatta
      901 accggtttat gggctgctgt aattaaggta aaactgaaaa taaatactat tgttatacct
      961 attattaata atgtatactt cattattttc acctccaaac aaaaaaagca caggataaaa
     1021 tccctgtgct gaatagttta atgactttgc ttttatatat cttatatttt taaaatcata
     1081 tccttatttt ataatattca attcttgacc aaccttttta aatttatcta aagcgaaatc
     1141 aaggtcgtct cggctgtggg tagctgaaat catgactcta attctggctt tgcccttggg
     1201 cactgtcggg aaaccaatcg actgggcaaa aacgctttct tcgaataact ttttagaaaa
     1261 ttcagatgcc agtttagctt tacccaacat gacaggtgtg ataggagtct ggctattacc
     1321 cacatcaaag ccaatctcct gcatcttctc tttaaagtaa tctccgttat cccacaactt
     1381 tttaactaga gagtcgtctt cttccaagat ttcaatcgac tttaaagtag ctgctacatc
     1441 aggtggtgtt acagctgaac tgaataaaaa tggccttgcc ttttgtttta gatactcgac
     1501 catcttagca cttccggctg cacaacctcc tacagtaccg attgccttag agaaagtacc
     1561 aacttcaata tcaacttcgt tgtgtaagtt gaagtgatct acaattccgc ggccgtgagt
     1621 acctaagact ccttcgccgt gggcatcatc aaccatggtg atagcattat atttttcagc
     1681 taactttact atttccggta attttgcaac atcaccatcc atactgaata caccatctgt
     1741 tacaattaag cctttacctt ctctttcttc ctgtaaaatt gactcgagag attccatgtc
     1801 tccatgctta tataccttta ttttagcgcc tgataatcta caggcatcaa taatactggc
     1861 atgatttagt tcatcactat agatcacatc acctttacca acaatagccg gaataactgc
     1921 taaatttgag ttatagccgg attgaaaggt taaggctgct tcaactttct tgaattcagc
     1981 tagtttttct tctaatttgt tgtgtaattc cattgttccg gcaattgttc taacagcacc
     2041 cggtccgacc ccataattca tcaatgcttt tttagcggcc tgcacggtct cgggatggtt
     2101 ggctaaaccc agatagttat tagaactaaa atttaatact tctttgcctt ccatttcaac
     2161 ccaggcaccc tgagggcctt ccagggttct tatcgtgtta tagagacctt cttcttttaa
     2221 ttgttcaagt tctttatcca taaagtcttg aaaatcttta ttcatctgtt aaaaccccct
     2281 aaggttttaa aattatcttg ccgcagttcc cttcatccat caatttcatt cccttttcaa
     2341 agtcttctaa aggaaattta tgtgtaataa caggtgaaag atctatcctt tcccctttta
     2401 gtaagttgct ggctatatgc caggtactaa acatctttct acctgtaaca cccacaactt
     2461 ttatgttctt aaatataata tcattggtta aatctaggct tactttatca tccggtaaac
     2521 cgagtaatga aacgattcca ccaccggtta ctgctttaat accatctctg attgcatccg
     2581 ggtggccgga catctcggct gtaaagtcta ctcctttttt accaaaaata tcatcaactg
     2641 cctctaaaag atcggtctcg atgggattta ttacatgatc agctcccatt tcaaaagaaa
     2701 gatctctacg atattcattg ggctcactta ctaaaatatt agctgcccca aaggtttttg
     2761 caactgcaat tgaaataagt cctactggtc ctgcccctgt aataaggaca tctttcccag
     2821 ctacattacc gggcaagatc gtatcaatag cattaccaag tggttcctgt atcgaagcaa
     2881 aatctttcgg aatactggga tcatttttcc agacatccac ttcttctaaa acagcatatt
     2941 ccgcaaatac tccatcctga tggactccaa agatcttcat gttttgacaa atatgttgtt
     3001 gtccggtctg acattgataa cagtgtccac agggaatatg ggtttcgctt gaaacaaaat
     3061 ctcccttttt aactttctct actctggaac ctacttctac tacttccccg gctacttcat
     3121 ggcccataat ttggggtggg ttgatatttt cttgggccca tttgttccaa atatatatat
     3181 gatgatctgt accacatata gaggtactct ctacttttat taaaacctgc tcttctccta
     3241 cttctggtat gtctacttcc ttatataccg ctccatactc aggtttttct tttacaacgg
     3301 catgcatttt acctttcatt atttaccccc ttagatagaa ttagagactt accatcacct
     3361 ccaaaatttt atacatccat ttatataact tcattattga ctttaaatat ccttcctaaa
     3421 ttataaatat tgcaatattt ttagattgca atattatatt tttgttgtta taattgtgaa
     3481 ggtttagaaa ttttcaggga gtgaactaat atggataata cttttaagac caagtactta
     3541 attctaacag gtctctttgc agctctaacc gcaatcgggg cttacattta tataccctta
     3601 cccataagtc cggtgcccat cacgctgcaa atgttcttta catttatggc gggtggactc
     3661 ttaggaaaaa aatatggctt cttttctcaa ttgatctata tattgatcgg agcaattgga
     3721 ctaccagtct ttgccggtgg taccggcggt tttggtattt tattcggtcc aaccggaggg
     3781 tatgtattag gatttttagc tgcgggatat attgctggct ttagcaaaga tacttttatc
     3841 tcaaagcttt tatatatgct tttagcttta attgtaattt acttgttggg tacaacccgc
     3901 ttgatgatgg ttatacaagt tgactttata aaagccttaa cgataggggt tacaccattt
     3961 ataataggcg accttatgaa agtagcattg gccgcctatc tatcggtgaa actatctaat
     4021 tatattcgat attaatatta ctggcagggg tagatatctt tattttaatt cgattatctg
     4081 cctctccata attcttagat tgatatacat cttcataaag ctggattaaa ccgctttctt
     4141 caaaattgtt gttatttatg acggtagaag tttctacccg gagagcggtc tgggcaggaa
     4201 cgatcaattt aatattacct gctccagctg agatctctgt ttcattatca taatcaggat
     4261 atctgattct taactgtcct aaaccggtat caatatctaa attagcaact tttaactctt
     4321 ctaaatccat ccagagatcg ccggctccag atttaacctg catatcaatc ggtattctat
     4381 cagttaaact gacattccag gtttcagatc catcagtacc tatattaacc tttttagcct
     4441 gttcaatttc aagttcagct ctactgtcag ctctattata ataaattttt gggctttctc
     4501 ccctgtaatc taaatttatg tcatagagaa aatcatcatt actgaccttc cctacattta
     4561 aacggtttgc tccatatcta aattcaacat ctaatttttc aatactgcta tcaaactctc
     4621 tatcttcctg ataggaaccc gaatattctc gatctccgaa gttgaaataa aggcggggag
     4681 agtcaagtct ccttaacata gaatcaggta ttacaattgc gccaatgaaa atcacaaaga
     4741 ttaagattgg aacaatccac cagagagctg tcttttttaa aattatgttt aagcctatta
     4801 atattaagat aacaggccag aatttaaaga gaaagtacca gacctgaaag gtggcaatcc
     4861 ccattgtatt taagagaaag aggagtccaa ttccaattaa aatcagtcct atgatatagg
     4921 cagatctatt tttattctga ctgctcattt tcctctgacc tccctataaa tcagcacgac
     4981 acccaggaca ataagtatta caggccagaa ccttctccag taaaaaccgg gaatccagtt
     5041 atcaataata aagaaaatac caagtgccac taaaattagt ccgaggattc tttttcgctg
     5101 ttctttactt tttttttcct cttctggctc ctgggcagat gccttatatt caccttcttc
     5161 atcttcttta tcaatttcta catcatatac ttcttcctcg cttttttgag tgccttctgg
     5221 tctctcggga ataataatcc aggcgatgat ataagctata atacctgccc cttcgataag
     5281 tgcaaaaagt acaaaagcaa gtctaactaa agttgaatca atgccgaaat actctgcaat
     5341 acctccacaa acaccaccaa taatttgatc tttttttgaa cgatataatt tcttgtccat
     5401 ctttatcctc ctcatataat ataattaatc taatatataa taaattaata aaaaattact
     5461 aaccgggatg aatatgaaca ataacctctt ttacttgttc acattctttt aataaagtat
     5521 ttcttacttc tactgaaata tcatgtccct gtttaacggt taaattacca tcaacaacaa
     5581 tccttaaatc cacatacaga tcaaccccac tataatgggc tcttaattct tttatctctt
     5641 taacaccttc tatatcattg acagtctttc ttatatcttc gctatcctct ttgggcgggg
     5701 ctttgaccat cagagtatta acagcatcta caataatatt ccagcccact ctaataatta
     5761 aaatcgctac tattattccc gcaattgggt ctaagatcgg ataacccata ttagctccca
     5821 gaataccaac agcggcagct atagaagata ttgcatcact tctatggtgc caggcatctg
     5881 ctatcagggc ctgattatta gtttcttctc ccactttatg ggtgtatcga aacattagtt
     5941 ctttgactat tatagaaact accgcggccc aaaaaccgta aatacctggt actccaaagt
     6001 tttggtttaa tatttgtctt aatatattat atcctaaagc cagaccggtt aatagtaaaa
     6061 gaatgcctag tatcttagct gcaatcgatt ctgcctggcc gtgaccgtat tgatgctctt
     6121 catccggtgg tttgctggag acataaatcg ctattaaaac agccagtgtt gaagccacat
     6181 cggaaaccga atgaaatcca tccgcaatta atgcagaact ggccactaga gtacctataa
     6241 ttatttttaa taaagctaaa aatatattcc cagaaatcgt gatatatgag atcttttttc
     6301 cctgactata tctgtccata ataatcaact ccaaatatta aaaggaatta gctatttaat
     6361 cttgaataat tattttaagc aataaatcta tcaaatgaaa taggtgatta acttgtcaat
     6421 aactacaatt gtacttttat gtatggtcgg ctttgcagca gcttttttag attctatagc
     6481 cggaggaggc ggcattttaa ccgttccagc ccttatggcg gccggacttc cacctcatct
     6541 tactttaggt actaataaat tttcttctac caacgcctct tttacaagta ctttaacttt
     6601 tttaaattct aagaaaatcc acatacctct gatcaaatat attctcccct tcagcttttt
     6661 aggggctgtt ttgggagtca gaaccgtatt acaaattaat gaagaattct taagaattat
     6721 tatcctggta cttttaattt ttgtagccct aaatacttta tttaataaaa atgtcggtac
     6781 cgaaaataat tttcaggggg tcaataaaaa gactatagta atcggttgta ttgtcggatt
     6841 tacgatcggt ttttacgacg gttttttcgg acccgggacc ggatcaattt tactttatgc
     6901 ttttatccga ttttttaaat ttgatttcac aattggaacc gccaatgcca ggatattaaa
     6961 cttcaccagt aatattacgt ctctagtcct atttgcttac tacggaagaa tagtctatgc
     7021 cgtaggtatc ccgatggccc tttctatgat cctaggtgcc tttttgggta ctagaatggc
     7081 tattaaacac ggcggtaaga ttattaaacc tatctttgtt accatgtctt tagcaatagt
     7141 agtcaagtta tttttagata ccatttaatt aatcaaagta gatatcctca tcagcttcat
     7201 taaattgagt tttatatagc ctattatata ctccaccttc tttattgata aggtcttggt
     7261 gagtaccctt ttcgacaatt ccatcttcag tcagcacgat tattttatca gcatttttaa
     7321 ctgtagaaag ccgatgtgca ataactaagg tagtacggtc ctttgataat ctgtctaaag
     7381 aagcctggat tattttttca ctttcattat caagtgagga agtggcttca tctaaaatta
     7441 aaatcggggg attctttaag aacgatcttg ctatcgaaat tctctgtttt tgccctcccg
     7501 ataatttaac accgcgttca ccaattttgg tttcataacc attacttaag ttggttatga
     7561 attgatgggc atttgctttt acagccgctt cttttatttg ttcttcgctg gcattaaaat
     7621 ttccatagga gatattttct tttatatttc cattaaaaag gaaaacttcc tgctggacaa
     7681 taccaatatt ttcacgtaat gattctagtt ttatatcctg aatactggtc ccatctatcg
     7741 taatttcgcc actatctatt tcatagaagc ggggcagcaa attacataaa gtagttttac
     7801 ctgcacccga aggaccgaca aaagcaaccg tttcacccgg acttatgtcc aggttaatat
     7861 tttttaatac ctttgagtgg ttgtcataac caaaggtcac atcatcgtat tttaatttgc
     7921 cctcgatatc ctctaactta acagcatcag atccatcttc gatttctggt tttatggcca
     7981 aaagttctaa aaatctatta aaaccggcca tacctttttg gaactgctgg ttgaagttaa
     8041 ccagtcttct aatcgggttc ataaacatat ttacataaaa taagaaagca acaagttcac
     8101 ctgtcgttaa agtatcagtg aaaaggaagt aggcacccgc tccgagagta aaaaatctaa
     8161 ttaaattaat gaaaaagttc atcccggaat gaaactgggc catattccgc atagctactt
     8221 cgcgggactg tctatactct tgatttccat catcaaattt atcttcttct aatttctcat
     8281 tagtaaagga ctttactact ctgataccgc tgagactgtc ctcaacaagg gagttgactt
     8341 ccgcaacttt ttctctgata tctttaaagg accgatacat ctttttacct aatattaaag
     8401 aaaagtaaaa cataatcggt agtaaggcaa aggtgaataa agccaactgc catttcataa
     8461 ataataaaat ggtaaaagat cctaaaatca taacggtaga tataaataag tcttccggtc
     8521 catggtgggc caattctgaa acattattta aatcgtttac gattctagac atcaagtaac
     8581 cgacttttgt attgtcataa aatttaaaag aaagtttttg aagatggcta aacagttctt
     8641 ttctcatatc tgtttccatc ctaataccaa caacatgtcc ccaataatgc acaatatact
     8701 ggaaaattag tcttaaaaca tataaaataa gtaatataaa caacattctt ataatcatag
     8761 aagcattttt attaggaaaa taattatcaa gaagtctctg gacaattact ggatataata
     8821 aatcaatact ggacattaaa aaggcacaaa caatatctaa taaaaacagt ttccaatggt
     8881 ttatgtagta ctttgaaaat ttcctgatca ttttatagca ttacctccta ttacttctaa
     8941 tattctatca tatttttaaa acaactgcaa agtattttat tgaatattat ttattttaat
     9001 ataaacttta ttaattttta ataaattttt aataaattta tttcattaaa acttttaatt
     9061 attaaagaaa ggggttgaaa attaatggaa ctttatcctt taaaattttt tccaatttat
     9121 aaaaataaaa tctggggagg taatcgctta aaatctaaat taaatagaga tatcaaagaa
     9181 gaaaacgtag gtgaaagctg ggaaatatct actaataagg atagtattag tatagtaaaa
     9241 aatggaaagc atgaaaacaa aaagttgaca gaattgattc aggaatttcc caaagaactt
     9301 ctgggagaaa attatagttc taatgataaa ttccctctcc tcttgaaaat aattgacgct
     9361 tccaaaaaac tatcggttca ggtccatcct gatgataaga tggcccagga attagcaaac
     9421 ggtaatggga aaaccgaaat gtggtatgta ctggatgcca acaaagaagc aaaactatat
     9481 attggcctaa aagatattaa aggtaaaaat cacttaagag aagtaataga aaaaggtcag
     9541 ttacaaaatt atttaaacac aatcaatgta caaaggggag atttcttcta tattccagct
     9601 ggtactgtcc atgctattgg gggaggctta atgttagtag aaatccagca gaattcaaat
     9661 accaccttcc gcttatatga ctggaatagg gtcgatgaaa atggcgaatc tcgcgaacta
     9721 catcttgaag acgggattaa agcaaccaat ttaaaggccg atgtggaaag atatgaatct
     9781 atctataaaa aagagactcc cggctataaa agaaaagtat taacaatttc aaattacttt
     9841 gtttctgaaa aggtaaccgt caaaaactcc ttccaattag atcatgataa ttttgcttta
     9901 ataacctgta tagaaaataa tgggttaata aaatatggca agaataaggt tgaaccttta
     9961 aaagaaggag aatcggttat gattccggcc ggtttagaag atgtcgaaat agaaggaaaa
    10021 atggaatttc tctatatgta tattcctaaa gatgtaaact attgtataca aaggttaaaa
    10081 agtaaaggct tcacttcctt taatctttaa tcttatccaa taactaaaaa gaaaaaatat
    10141 aatacctatg tttaaataca ataattgatt ataaaaaggg ttaatttcct ttttataatc
    10201 aatttctttt tttatgttct tattcctgat ttctactaaa ctttaatctc aatataatag
    10261 caactaagtt gattaataat accaaactta gtaagaccac cgcagacccg taggcaattg
    10321 gaacctgttt tgaaggaaaa gttccagctg tgacaagggc atatatatta taaggtaggg
    10381 ccataacttc atcaaaaaca gaatccggta atctcatttg gaagtaggta gcagctgtaa
    10441 atataatggg agctgtctca ccagctaccc tcccgattcc taagataaca cctgtcaaaa
    10501 tacccggcat agcggaaggc agaacaacat ttttgatagt ctgccagttg gtagcgccca
    10561 ttgcaaaaga agcatgccga tattcatcgg gaaccgccat taatgcttct tccgaagccc
    10621 taataatagt tggcaaaatg actattgcta aagttaatga acccgataat attgaaactc
    10681 caaatcctaa aaatttaaca aatataccga ggccgaaaag tccaaatact actgagggta
    10741 ctcctgccaa gttatttaca ccgatgcgaa tagcagtaat aatcttaccc ttatcagcgt
    10801 attctgtcat atagatggct gcaaataccc caagcggggt tgcaaataat atagctccaa
    10861 ttactaaata aaaggttcct acaatagcgg ggaatatacc gccttccgtc atcccattac
    10921 gaggcatggc agttagaaat tcccagtcaa ttactcctat gcctctcata aaaacaaagt
    10981 agatgatgat ccctaagata aataatacta aaagaagggt gattcctaat attgaaaaga
    11041 caatcttttg tctggaatgt ctcttttcaa gattaatatc agtgtctctc attgcccact
    11101 caccttcttt ttaaaccttc tactggccag atctgccaca atattaaatg ttaaagttat
    11161 aataaataat ataatagcta ttccaaataa ggcctgatag tgttcacttc ccaccggggc
    11221 ctcacccatt tcagctgcaa tagttgccgt cattggtcgt accggtttta gaaaactaga
    11281 aggcattgcg gctgcaccac cagcaaccat gagtacagtc attgtttcac caatagccct
    11341 tcccatacct aaaattaccg cagttactat tccagaactg gctgtgggta agattacttt
    11401 actgattgtc tcccagcgcg ttccacctaa agccaacgaa gcatctctat acctttttgg
    11461 tatggaagta attgcatctt ccgacaggct tacaatagta ggtaaagcca taatgccgag
    11521 cataatggaa gcatttaaag ctgttaatcc ggttggaaga ccaaatacat cttttaaaaa
    11581 cggacccaat attttcattc caaataaacc atatattaca gacggaatac cagctaataa
    11641 ttcaatggct ggtttcacaa atttttttaa gtttttaggt aaaacatatg atatatagac
    11701 ggaagaagcc agtccaatcg gtacagctaa aaccatcgaa ccaaatgtaa cccataaaga
    11761 agctgagagt aatggcaaaa taccataagc tggtggatca taagttggat accaatcttc
    11821 tccaaatata aaatcaaata cccctacttc ttgaaaaatg ggtaaacctt ccctaaataa
    11881 aacgaataca atacctgtta aaaatatgat ggaagataaa gcaaaaaata atagtatgta
    11941 ctttattatt ttttcctttt ttctccaatc catataaaca cccccacata aaataataca
    12001 atatttaaat atgtaaatta agggaggata taatatcccc ccttgtttaa tcagagttga
    12061 attatattat ctattagaat aagggaacaa atccttgttc ctctactact ttttgacctt
    12121 cttgactgag tacaaagtta atgaatctaa gtgttaatcc ttctggccaa ccatcagtaa
    12181 acataaagag cggtcttgct atcgggaatt ctccacttgc cacagtacta ttactgggag
    12241 caactccatt tacttttact gctttaagtg tatcatctaa gtaacctaat ccaacatacc
    12301 cgatggctcc ttcagtatca gatacaacac tagaaatagc accattagag gcctgtagta
    12361 aagcattagg agtaagtctt tcgccttgta atgcaatttc tccaaaaact tcaaatgtac
    12421 ctgaactgga gtctcttgaa attacaacta tttcctcatc attaccgcct aattcgctcc
    12481 agttggtaat ctcaccagta tatatttgtt ttatttcatc taaggttaaa gattctaccg
    12541 ggttagcagg atttataact actgcaattc catccattgc tacacggtgt ggtacaggat
    12601 aataaccatt atttacagca ttttctactt cactttgttt aataaatctt gat
//
LOCUS       DATGCJ010000233         6757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_35933, whole genome shotgun sequence.
ACCESSION   DATGCJ010000233 DATGCJ010000000
VERSION     DATGCJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35933"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..658
                     /locus_tag="VKN64_10335"
     CDS             182..658
                     /locus_tag="VKN64_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60682.1"
                     /translation="MEYNFKKMKEEYANDIAYDWKYTRKYSFYDMKDDQEDLEEFLDP
                     DNWENKFAVLNKNDELIDFYSYYFKDEIMWIGFGLRPDLTGKGLGESFVKAGIKHGID
                     KFSYSKDHIKLAVAKFNERAIRLYQNIGFQAIDEYDQKTNGGTYRFIKMKKLLNFY"
     gene            724..1284
                     /locus_tag="VKN64_10340"
     CDS             724..1284
                     /locus_tag="VKN64_10340"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60683.1"
                     /translation="MAPLIDTFPEYNIGEIILRKLNKEDIDDMLKYLNDKEVNKYLPD
                     DAKMDNRAEVKEMLEAVNQGFAESNKIRWGIIDKNSSRVVGDCGYYKIDLKNKSGEIS
                     YRLARPYWGKGIMSQVLNKMLYFGFEEIHLNRIEASVIPGNKGSIYLLEKNGFIEEGV
                     LRDALVKDDNYHDLIVYSILKRDYFE"
     gene            1407..1985
                     /locus_tag="VKN64_10345"
     CDS             1407..1985
                     /locus_tag="VKN64_10345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60684.1"
                     /translation="MIEGKRVIIRQLELGDEELLYKWRNNQEGNLYCGFKYGFLLSEE
                     AFRLKVKKQVGNTDIFPNEKTFIILKKEDLKPIGDISYRNWDKRNRSAEFGIEIGEIN
                     ERGKGFGFDALSHFLQFMFNHLNLNRIELTTLADNEKSIKLYKKLGFKEIGLMRESSF
                     DSRTGQYSDVLYMDLLKKEWSLIDAETDTRRV"
     gene            1957..2604
                     /locus_tag="VKN64_10350"
     CDS             1957..2604
                     /locus_tag="VKN64_10350"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMA60685.1"
                     /translation="MLKQIQEEFDKIKGKLNSKKRNKVQRKLYRRLPDKKEELIELFD
                     KLVNKGDWHVFWLVTIWIKRKKLYNKPYMPYYEVWLYNNIKSWGSCDVFCYRVLNPML
                     ERHPEFYKKILKWTESELTYVRRAAPVSLLESTRSFKVNYPFDKVLKVVNRLKDDQEY
                     HVQKGVGWLLKYTYLSYPDQTYDYLKKNVDDLARVIFRYALEKAPEKEKTELMKL"
     gene            2622..3140
                     /locus_tag="VKN64_10355"
     CDS             2622..3140
                     /locus_tag="VKN64_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60686.1"
                     /translation="MRVEHKGQTYEVEFKKIVNKKYNKQVKKLFREYAEELEIDLDFQ
                     DFEDELNNLPGKYSPPDGLLTLAFLANQSVGCIALRKISEEICEMKRLYVKREYRGFG
                     IGRELVNILIKEAKHLDYKFMRLDTIPSLVKAQKLYESFGFYDIEPYVYNPTEGARFM
                     ELDLNSIELDNI"
     gene            3201..3749
                     /locus_tag="VKN64_10360"
     CDS             3201..3749
                     /locus_tag="VKN64_10360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60687.1"
                     /translation="MFKFKVDDDLYLKLFNKNEAEQLFKLIDSNREHLKKWLPWVEDV
                     KEPSDSEDFIKNSRQQFADNNGFQAGIWYKNKLVGVIGYHSIDWSNKKTSIGYWLGKE
                     YQGTGIITRCCEALVDNAFENYDLNKVEISCAEGNVRSRSIPERLGFIQEGTIREAEW
                     LYDHFVTHIQYGMLAAEWKERK"
     gene            3772..4695
                     /locus_tag="VKN64_10365"
     CDS             3772..4695
                     /locus_tag="VKN64_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60688.1"
                     /translation="MKKLDKKYLSMIDKYLKDHCRLLEKKLFEYYFKDAKPKEVVNAL
                     KLYQNQDGGFGQGLESDFRMPDSSPMATSIGVRILKDFDSSKKSKMMLRRAVKFFESS
                     FDENRHGWYALTEVVNDYPHTPWWHFDEEKGMTVIDENWGNPSAEIIAYLYKYHSYLN
                     KLDVKELVSHAIERLNNKKEFNSENEVSCYIELYKVLPKNVKQEMYEPLSNAISDVIE
                     YDEDKWREYVPLPLNFISSPSDEFFDIEKEKIEKNLDLYIDLIEENTVINPPWGKSFY
                     TEGLKRAYNEWKGVLTVHALKVLDNFNRIEK"
     gene            4737..5507
                     /locus_tag="VKN64_10370"
     CDS             4737..5507
                     /locus_tag="VKN64_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMA60689.1"
                     /translation="MQKKEFPILEFDPTKRAVIEPGDHIEKVEMPASCVVCFFQEVIT
                     KLKEEDRLDKLAAIETEMGRMPIYEIKLEDSKNITVFHPGIGAPLAAGMIEEVTALGG
                     KNFIACGGAGVLKKDIAVGNLIIPNSAIRDEGVSYHYVEPGRELKPTRKALSILEETL
                     TNQDIDYVMGKTWTTSSFYRETQKKVNLRRDEGCLTVEMESAAFFAVAEFRDLEFAQI
                     LYGGDDVSGEFWDKRNWAKRKSIRENIFWLAVKAALKF"
     gene            5537..5908
                     /locus_tag="VKN64_10375"
     CDS             5537..5908
                     /locus_tag="VKN64_10375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMA60690.1"
                     /translation="MIERVLIPPYDEYDLSTFSISNGIVQIGHFGGYVNDEGEVLETF
                     EEQFDQTLRNLESALSNIELTLNNVLKLTVILRDIDDFATMHKVWKKHFNEGSYPVRA
                     VITSDFVEDDILVQVEGSAAY"
     gene            5954..>6757
                     /locus_tag="VKN64_10380"
     CDS             5954..>6757
                     /locus_tag="VKN64_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HMA60691.1"
                     /translation="MVKYPKPLKKGDIIGVTAPSSGAPGVFSNKLDSAINKFKELGFN
                     VIETESVRKNNDNLVSAPAEVRAKEFNELYFNKEVRAIIPPWGGEFLMEMLPYLDFKK
                     LKESEPKWILGFSDTSTLLFTLTLNSNIATAHGPNFLDFGNNPVHESVLESLKILQKD
                     KGATFVQESLEYYQEEWSEVTEDTFPPYNLTEKVDWKVLREDTQAQFEGRLIGGNLDV
                     ICKLIGTPYDKVKEFLNQYKEDGFIWYFENCEMDSTDVRRTLWQMQMNGW"
BASE COUNT         2467 a          836 c         1422 g         2032 t
ORIGIN      
        1 tatatcctat gtggtaaaga ggatagagac tgtctggaat actgtgatat ttttgtagat
       61 aaattaagta agtttgatgt tggatataaa tataattaat agaaggattg gaacatgatt
      121 acccacagga ctttacaaag agattgaaac aagctttaga atatatagag gggtagttga
      181 attggaatat aattttaaaa agatgaagga agaatatgca aatgatatag cctatgattg
      241 gaagtataca agaaaatatt ctttttatga tatgaaagat gatcaggaag atttagaaga
      301 atttttagac cctgataatt gggaaaataa atttgctgtc ttaaataaaa acgatgaatt
      361 aatagatttt tattcctact atttcaaaga tgaaataatg tggattggat ttggtttaag
      421 gcctgatcta actggtaaag gtctaggtga aagttttgtt aaagccggga ttaaacatgg
      481 aatagataag ttttcatatt caaaagatca tatcaagtta gctgtagcta aatttaatga
      541 gagagcgatt aggttatacc aaaacatagg attccaagcc attgatgagt atgaccaaaa
      601 aacaaatggt ggaacctata gatttatcaa gatgaaaaaa ttattaaatt tttattaatc
      661 ctcaaaaaag catagaggat ttttgtaaaa tatagggtat taaaatacta tggggtgtta
      721 aagatggctc ctttaataga tacatttccc gaatataata taggggaaat aatacttaga
      781 aaattaaata aagaagatat agatgatatg ttaaaatatt taaatgataa agaggttaat
      841 aaatacctgc ctgatgatgc caaaatggat aatagagctg aagtcaagga aatgttagaa
      901 gcggttaatc agggatttgc agaaagtaat aaaatcaggt ggggtataat cgataaaaac
      961 agttcaaggg tagttggaga ttgcggttat tataaaatag atttaaaaaa taaaagcggg
     1021 gaaataagtt atcgattagc caggccttac tggggtaagg gtataatgtc tcaggtattg
     1081 aataagatgc tttactttgg ttttgaagag attcatttaa atagaataga agcttctgtt
     1141 atcccaggta ataaaggctc tatttaccta ttagaaaaaa atggttttat agaagaaggt
     1201 gtgctaagag acgctttagt gaaagatgat aattatcacg atttaattgt atattcaatc
     1261 ttaaaaaggg attattttga atgatttaaa ttatctataa tagacaattt aaatttatat
     1321 gtttaaatag ataaccctct cagcagggtt tttgtttttt aaaaagaaga aattcttatt
     1381 aataataagt agaggagatg gattttttga tagaaggaaa aagagtaata ataaggcagt
     1441 tagaattggg agatgaagaa ctgctgtaca agtggaggaa taatcaagaa gggaatcttt
     1501 actgcggatt taaatatggt tttctcttaa gtgaagaagc ctttcgttta aaggtaaaaa
     1561 agcaagtagg aaacacagat attttcccaa atgaaaagac ctttattatc ttaaagaaag
     1621 aagatttaaa gcctattggt gatatttcct atcgtaactg ggataaaaga aatcggagtg
     1681 cagaatttgg aatagagatc ggtgaaataa atgaaagagg taaaggcttc ggctttgatg
     1741 ccttatccca ctttttacaa tttatgttta accatttgaa cctaaacaga attgaactta
     1801 caaccttagc cgataatgag aagtcaatta aattatataa aaagttgggt tttaaagaga
     1861 ttggtctgat gcgagaaagc agttttgaca gtagaaccgg tcaatattct gatgtattat
     1921 atatggactt gttgaaaaaa gaatggagct taatagatgc tgaaacagat acaagaagag
     1981 tttgataaga taaaaggtaa gctcaattca aaaaagagaa ataaagtgca gcgtaaatta
     2041 tatagaagat tacctgataa aaaggaagaa ctgattgagc tttttgataa attagtcaac
     2101 aaaggtgatt ggcacgtctt ctggctggta acaatttgga taaaaagaaa gaaattatat
     2161 aataaaccat acatgccata ttatgaagtt tggctgtata ataatataaa aagctggggc
     2221 agttgtgatg ttttctgtta tcgggtatta aatcccatgt tagaaagaca tcctgaattt
     2281 tataagaaaa tcttaaaatg gacagaatca gagttaacct atgttagaag ggcggccccg
     2341 gtttctcttt tagaatctac aagaagtttt aaagttaatt atccttttga taaagtactg
     2401 aaagttgtca ataggttaaa agatgatcaa gaataccacg tacaaaaggg tgtaggctgg
     2461 cttttaaagt atacttattt aagttatccg gaccaaacat atgattacct taagaaaaat
     2521 gtagatgatc ttgcccgggt aatatttaga tatgcccttg aaaaagcccc tgaaaaggaa
     2581 aaaactgaac tcatgaagtt atagagattg aggtgagggt aatgagggtt gagcataagg
     2641 gtcaaactta tgaagttgag ttcaaaaaga tagtaaataa aaaatacaac aagcaagtaa
     2701 aaaaattgtt tagagaatat gcagaggaac ttgaaattga ccttgacttt caagattttg
     2761 aagatgaatt gaataattta ccgggaaaat atagtccacc cgatgggtta ttaacacttg
     2821 ctttccttgc aaaccaatca gtcggctgta ttgccctgcg taaaatatcg gaagagatct
     2881 gcgagatgaa aagactatat gtaaaaagag agtatagagg ttttgggatt ggccgtgagc
     2941 ttgtcaatat cttaataaaa gaagccaaac accttgatta taagttcatg aggttagata
     3001 ctataccttc tcttgtaaaa gctcaaaaac tttacgaatc atttgggttt tatgatattg
     3061 aaccctatgt gtataatcct actgaaggag ccaggtttat ggaattagat ctaaattcaa
     3121 tagagttaga taatatctaa tatagctttt tttatttatt tttttgaaaa taaaaaatta
     3181 gaaacagtga gggagtaaag atgtttaaat ttaaggttga tgatgattta tacttaaaat
     3241 tattcaataa aaatgaagca gaacaattgt ttaagctcat cgattcaaac cgggaacatt
     3301 taaaaaagtg gctgccctgg gttgaagatg ttaaagagcc ctctgattct gaggatttta
     3361 ttaaaaactc ccgccagcag tttgcagata ataacggctt tcaggccggt atctggtaca
     3421 agaataagct cgtaggagtt atcggttacc atagtataga ttggtctaac aaaaagacct
     3481 caattggata ctggctgggt aaagaatatc aaggaaccgg tatcataaca agatgctgtg
     3541 aggctttggt agataatgct tttgaaaatt atgatttaaa taaagttgaa atcagctgtg
     3601 ctgagggcaa tgtgaggagc aggtctatac cggagaggtt aggatttatc caggaaggta
     3661 caattagaga ggcagaatgg ctctatgatc attttgtaac tcatattcaa tatggtatgt
     3721 tagcagccga atggaaagaa agaaaataat tttatagaga ggatgtttaa agtgaaaaag
     3781 cttgataaga agtatctatc tatgattgat aaatacttaa aagatcactg tcggctttta
     3841 gagaaaaaat tatttgaata ttattttaaa gatgctaagc caaaggaagt tgtgaatgcc
     3901 ttaaaattat atcaaaatca ggatggaggt tttggtcaag gactcgaatc cgactttcga
     3961 atgccggatt cttcaccaat ggcaacttca atcggggtaa gaatattaaa ggactttgat
     4021 agttctaaaa aatcgaagat gatgttaagg agagcggtca aattctttga atccagcttt
     4081 gacgaaaata ggcacggctg gtacgcctta actgaagttg ttaatgatta tccccatact
     4141 ccctggtggc attttgacga ggaaaaggga atgaccgtca ttgacgaaaa ctggggtaat
     4201 ccctcggccg agataattgc ttatttatat aagtaccatt catatcttaa taaattagat
     4261 gtaaaggaat tggtaagtca tgccatagag agacttaaca ataaaaaaga atttaattca
     4321 gaaaatgagg tatcctgtta tatagaactt tataaagtat tacctaagaa tgtaaagcaa
     4381 gaaatgtatg aaccattatc taatgctatt tcagatgtta ttgaatatga tgaagataaa
     4441 tggagggaat atgtaccttt acccttgaac tttatctcca gcccatctga tgaatttttt
     4501 gatatagaaa aagaaaaaat tgaaaagaat cttgacttat atatcgattt gatcgaagag
     4561 aatacggtta tcaatccgcc ctggggtaaa agcttttata ccgaaggttt aaaacgtgcc
     4621 tataatgaat ggaagggagt tttaaccgta catgctttaa aagttttaga taattttaac
     4681 aggatagaaa aataattatt tatttgagat ttattaaaac aaggagtgtt taacaattgc
     4741 agaaaaaaga atttcccatt ttagaatttg atcctaccaa aagagctgtt attgagcctg
     4801 gtgaccatat agaaaaagta gagatgccgg ccagctgtgt ggtctgtttt ttccaggagg
     4861 taataacaaa gttaaaagaa gaagatcgtt tagataaact ggcagctatc gagaccgaga
     4921 tgggccgcat gcctatctat gaaattaaat tagaagatag taagaatata actgtgttcc
     4981 atcccgggat tggtgctccc ctggcagccg gaatgatcga agaagttact gctctgggcg
     5041 gcaaaaactt tattgcctgt ggtggagcag gtgtcctcaa aaaagatatt gcggtaggta
     5101 acttaattat acccaattcc gctatcaggg atgaaggtgt ttcttaccat tatgttgaac
     5161 caggccgaga attaaaacca actagaaaag ctttaagtat tttagaagag actttaacca
     5221 atcaagatat tgattatgta atgggcaaga cctggactac gtcttctttt tacagagaaa
     5281 cacaaaagaa agttaattta agaagagatg agggctgttt aacggtagaa atggaaagtg
     5341 ctgccttttt tgcggtagct gagttcagag atttagagtt tgctcagatt ctttatgggg
     5401 gagatgatgt gagcggcgag ttttgggata aaagaaattg ggctaagagg aaatcaatta
     5461 gagaaaatat attctggctg gctgtaaaag cagctttaaa attctaaaat atatctttaa
     5521 taggaggatc ttttaaatga tcgaaagagt attaatacca ccttatgatg aatatgacct
     5581 ttcaactttt tctattagta atggtatagt acagatcgga cattttggtg gatatgtaaa
     5641 tgatgaagga gaagtattag aaacttttga agaacagttc gatcaaactc tcaggaattt
     5701 agaaagtgct ttgagtaata ttgaattaac gttaaataat gttttaaagc taactgtaat
     5761 tttgcgagac atcgatgatt ttgcaactat gcataaagtt tggaaaaagc attttaatga
     5821 aggaagttat cccgtgcggg ccgtcataac atcagatttt gtagaagatg atattttagt
     5881 acaagtagaa ggctctgcag catattaata tttttccatt caaaatttaa attaaggggg
     5941 cagctgtata gaaatggtca aatatccaaa accacttaaa aaaggagata taattggagt
     6001 aacagcacct tccagcggtg ctccgggagt tttttctaat aaattagata gtgctataaa
     6061 caagtttaag gaacttggat ttaatgtaat tgagacggaa tcagtcagaa agaataatga
     6121 taatttggtt agcgctccgg ccgaggtaag agccaaagaa ttcaatgaac tttattttaa
     6181 taaggaagta agggctataa taccaccctg gggtggagag tttttgatgg aaatgctacc
     6241 ttacttggat tttaaaaagt taaaagagtc tgagccaaaa tggattttag gtttttctga
     6301 cacaagtacc cttctattta ctcttacttt aaattcaaat atagcaactg cccatgggcc
     6361 taacttttta gactttggaa acaatccggt gcatgaatca gttttagaga gtttaaagat
     6421 attgcagaaa gataagggag ctacatttgt acaggaaagt ttagaatatt atcaagagga
     6481 atggtcagaa gtaactgagg atactttccc gccctataat ttgacagaaa aagtagattg
     6541 gaaagtccta agagaagata ctcaagcaca gtttgagggg cgtctgatcg ggggcaatct
     6601 cgatgtaatc tgtaaactga tcggtactcc ttatgataag gtgaaggaat ttcttaatca
     6661 atataaagaa gatggtttca tctggtattt tgaaaactgt gagatggatt caaccgatgt
     6721 taggagaacc ctctggcaga tgcagatgaa cggttgg
//
LOCUS       DATGCJ010000234         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_304576, whole genome shotgun sequence.
ACCESSION   DATGCJ010000234 DATGCJ010000000
VERSION     DATGCJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304576"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1606
                     /gene="aroA"
                     /locus_tag="VKN64_10385"
     CDS             308..1606
                     /gene="aroA"
                     /locus_tag="VKN64_10385"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMA60692.1"
                     /translation="MIIISLKVEKKEKLKGKVSIPGDKSISHRAVILSSLAKGKSKIE
                     GFLESEDCLSTVKAFRNLGVEIKKESSNQYIVKGVGLNGLKEPDNILDCCNSGTTMRL
                     MLGLLSAQNFYSILTGDDSLRARPMERIIKPLTRMGAHIWSREGGYAPLSIMGSNLTG
                     IDYKMPVASAQVKSAILLAGLYSDQSVKIVEPALSRDHTERMLAGFGINIKKEGHHII
                     LPADNNRKLHPQEFVVPGDISSAAFLISAGLITNNSQILIKDVGINPTRTGFLEVVEK
                     MGGQIKLLNRREVSGEPIADIEVKSSQLHGIEIAGELIPKMIDEIPIIAVLASQADGV
                     TVIKDAGELRVKETDRIRAIVSQLSRLGVKMEEFRDGMIIKGPNQIKGGISVKSFGDH
                     RIAMSLAVAGLVAEKELTITNSRVINTSFPGFKETLFSLS"
     gene            1692..>2111
                     /locus_tag="VKN64_10390"
     CDS             1692..>2111
                     /locus_tag="VKN64_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMA60693.1"
                     /translation="MNTGLYKRVRDNVKGMKKYKPGKGIEEVKNEYGLDKVIKMASNE
                     NPLGHSEQVLNCLQESACNFHQYPDSNCNQLKNCLADFYNINKEKLFIGNGSDEIIDH
                     IMTVFVEKGDEVILADPSFIKYKLAVQASGGISKEIPL"
BASE COUNT          724 a          330 c          465 g          592 t
ORIGIN      
        1 agccaggggt gctgggacct ggtaatttcg aaagttgaaa tacatcctgg agttgtaggc
       61 cgaatataaa agtaagcctt accgtaatcc gtcgttatcc ggtgagggag tgtttgctcc
      121 caaaaagaga taatctattg tttatagatt aaatcaggtg gtaccgcgtt caagtcgccc
      181 tgtagtaggg gcggcttttt ttagttttaa aataaatttt atgcaatgta ctattcttta
      241 ataaatctta aactgaagtt ttataaggac tctgggaatt tgaatactga actacttaaa
      301 aaggagagtg attataatct cattaaaagt agaaaaaaaa gagaaactta aaggtaaagt
      361 ttcaataccc ggtgataaat ctatatccca tcgggctgta attttatctt cacttgcaaa
      421 aggaaaaagt aaaatcgaag gatttctaga gagtgaagat tgtttaagta cagtcaaagc
      481 tttcagaaat ctaggagttg aaattaaaaa agaaagcagt aatcaatata ttgttaaggg
      541 agtaggccta aatggtttaa aagaaccaga taatatattg gactgctgca attcgggtac
      601 taccatgcgc ttaatgttag gtcttctatc tgcacaaaac ttttattcta tactaaccgg
      661 tgatgactct ctaagagcac gccctatgga aagaataatc aaacctttaa caaggatggg
      721 agctcatatt tggtcaagag agggtggtta tgccccgtta agtattatgg gaagcaacct
      781 gacaggcata gattataaga tgccggtcgc cagtgcccag gtaaaatcag ccatactact
      841 ggccggactt tatagtgatc aatcggtaaa gatagtcgaa cccgctttat ccagagacca
      901 tacggaacga atgttagctg gttttggaat caacataaaa aaggaaggtc atcatataat
      961 acttcctgct gataataata gaaaattaca tccgcaagag tttgtggtac ctggtgatat
     1021 ttcttctgca gctttcctta tttcagccgg gttaatcaca aacaacagtc aaatattaat
     1081 aaaagatgta ggtattaatc ccacccggac cggtttttta gaggtagtgg aaaaaatggg
     1141 cggccaaata aaattgctaa acagacggga agtaagtggt gaaccgatag ctgatattga
     1201 ggttaaaagc agtcaactgc acgggataga gatagcgggc gaacttatcc ccaaaatgat
     1261 agatgagata cctattattg ctgtattagc cagccaggcg gacggagtaa ctgtgattaa
     1321 agacgcgggc gaattaaggg ttaaggaaac tgatagaata agggctatcg tatcacaact
     1381 gtcccgttta ggtgtaaaga tggaagaatt tagagatggt atgattatta aaggccccaa
     1441 tcaaatcaaa ggtggaattt cagtaaaaag ttttggcgac catagaatag cgatgtcgct
     1501 ggccgttgct ggtttagtag ctgaaaagga gttgacaata actaacagcc gggttataaa
     1561 tacttcgttt cccggtttta aagaaacgct attttcttta agttagttat ttcctcctta
     1621 attcttttaa cttaagtatg agaatatttt tcttattaaa aaaacaaaaa atagaaagga
     1681 agggtgtaac gatgaacacg ggcttatata aaagagtacg agacaatgta aaaggcatga
     1741 aaaaatataa accgggcaag ggaattgaag aggtaaaaaa tgaatatggt ttagacaagg
     1801 ttataaaaat ggcttctaat gaaaaccccc tgggccactc tgaacaggtt ttaaactgct
     1861 tgcaggagtc tgcttgtaac tttcatcagt atcctgacag caactgtaat caacttaaga
     1921 attgcctggc tgatttctat aatataaata aagaaaagct atttatcggt aatggttcgg
     1981 atgagataat cgatcatata atgacggttt ttgtcgagaa gggagatgaa gtaattttag
     2041 ctgatccttc ctttataaaa tacaaattgg ccgttcaggc cagtggaggg attagtaaag
     2101 aaattcccct g
//
LOCUS       DATGCJ010000235         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_394643, whole genome shotgun sequence.
ACCESSION   DATGCJ010000235 DATGCJ010000000
VERSION     DATGCJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394643"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..500)
                     /locus_tag="VKN64_10395"
     CDS             complement(102..500)
                     /locus_tag="VKN64_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HMA60694.1"
                     /translation="MKIDNRLIINRPLQPNKQNNKVNNDDNKKVNKNNTPFKKIFQEK
                     IKNSKIEFSKHAKERLQARNINLNKTQMQKLQRGVEKAESKGAQDSLILVDDVAYIVS
                     VKNNTVVTAIDGNNMKEKVFTNIDSTVLMK"
     gene            complement(528..947)
                     /gene="flgD"
                     /locus_tag="VKN64_10400"
     CDS             complement(528..947)
                     /gene="flgD"
                     /locus_tag="VKN64_10400"
                     /inference="COORDINATES: protein motif:HMM:NF007197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HMA60695.1"
                     /translation="MPIGAAGAVNSQAAANDQAIGNQVKKELDRDAFFKLLITQLQNQ
                     DPLKPMEDKEFIAQMAQFSSLEQMNNMNQNFNKLMDVQRINQNSSLIGKTVEKEGSKG
                     TIKGKVIKISFEEDKTYAHLDNENKSKINVDNITAVY"
     gene            complement(966..2060)
                     /locus_tag="VKN64_10405"
     CDS             complement(966..2060)
                     /locus_tag="VKN64_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HMA60696.1"
                     /translation="MLADKLDNKKTNQQSKLISKIKVSNNSQLNENIKIQKILTEINK
                     LTNKEIKTIQENIVKQINNSSKNKQNQTNNIIKNTNLNTEQVSKDKAKQESTNKNKKI
                     NHNKPNNKTSNFKNIKVAGDNLKDTSQVKTRDNTKEKITFNNLEFEIKSKESNKKYKK
                     VNNLKFFSENQNNQGKENITGLKSFDIKNNTNQQNSTIQFKNLIKENNVLDQIKNKVN
                     IKNLSNQNQQIEIKLKPDSLGKMKINLSMESNKIVGKILVENNIVQNYLENNLQELKN
                     NLLSKGLQIDQFNIESETKDFNHNHNNQDSNSYFQQFKEGHEKNNQKRQSNVYEFLRN
                     NESSEGNVEQIEKAGWGTLETHKSGFEYFA"
     gene            complement(2706..3227)
                     /locus_tag="VKN64_10410"
     CDS             complement(2706..3227)
                     /locus_tag="VKN64_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HMA60697.1"
                     /translation="MIKKAIYIFLFLFLTVYIIWALNLFGILQLDDVVTSTPYIKERM
                     KTDKQYDRMMNDYYKLENAYDELKTANDELKSELQQVKNTIEQKNEKIKTLENRYSEL
                     KQSQEEEKNRVEKLVKIYENMDPEEAANVITDLRMETTLAIVNELKDEHLAEILMNMP
                     QDKAVQLTERLRE"
     gene            complement(3229..3663)
                     /locus_tag="VKN64_10415"
     CDS             complement(3229..3663)
                     /locus_tag="VKN64_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HMA60698.1"
                     /translation="MAKFNFRLQRVLDIRDVELDKAQNELYQKRQKEKEIKEELENAK
                     EKLSNLFDYIRYNSLTVTENIRTREYIKKQQKKIEEISNRYHRQKEVVKEYQEQVIEK
                     KQAKEKIVKIKEKKSEAFYKDFLDKEQKELDELSLRLSQQGV"
     gene            complement(3690..5012)
                     /gene="fliI"
                     /locus_tag="VKN64_10420"
     CDS             complement(3690..5012)
                     /gene="fliI"
                     /locus_tag="VKN64_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HMA60699.1"
                     /translation="MFDDLNLKKLEETIENTKINREFGYITRVVGLIIESVGPEVSIG
                     ELCYIKSTEGDIEAEVVGFNDNKVLLMPIGVMKGITPGAKVIASGSQLKVEIGNELLG
                     EVLDGLGRPLSSDKDEFNKATLKEVNVNKEPPDPLKRQRIKEPLSLGIKSIDGLLTCG
                     RGQRLGIFAGSGVGKSTLLGMAARNTDADINVIALIGERGREVRDFIEEDLGPEGLKK
                     SIVVVATSDKPALIRMKAASVATAIAEFFRDQGKDVLLMMDSVTRVAMALREVGLASG
                     EPPATRGYPPSVFAKLPKLLERTGTNDKGSITALYAVLVEGDDFNEPISDTVRGILDG
                     HISLSRSLASKNHFPAVDVLESVSRVMKDVVTAEHNENANQLKKLLADYKEAEDLISI
                     GAYERGSNPKVDRAIDKIDRINDFLTQRIEEKVSFSETKNRLAQIINS"
     gene            complement(5016..>5252)
                     /locus_tag="VKN64_10425"
     CDS             complement(5016..>5252)
                     /locus_tag="VKN64_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HMA60700.1"
                     /translation="LSNNHQQFVINVNPEMLPYLSKTELKTSFPESKFEFNADDSLQK
                     GDCVINTNFGGKDAYLTEKLTRLEKQIKKEMGMS"
BASE COUNT         1241 a          950 c          751 g         2310 t
ORIGIN      
        1 aattatcaac ctttccttta cgctggcaga atgcctctgt cagttagcac tctgtggctc
       61 ccgtaaaggg tcctgccatc taaccagctt aatttttatt tttatttcat cagtactgtt
      121 gaatcaatat tagtaaagac tttctctttc atgttattac catctattgc tgttactaca
      181 gtgttgtttt ttacacttac aatataggca acatcatcaa ctaatattag ggaatcttgc
      241 gccccttttg attcagcttt ttctactcct ctttgtaatt tctgcatttg ggttttattc
      301 aaattaatgt ttctcgcctg taatctctct ttagcgtgtt ttgaaaactc aatctttgaa
      361 ttcttaatct tttcttgaaa tatcttctta aaaggagtat tatttttatt aactttttta
      421 ttatcatcat tgtttacttt attgttttgt ttattgggct gtaggggtct gttaattatt
      481 aatcgattat ctattttcat ttttctcacc ttactcccct ttacttttta gtatacggca
      541 gtaatattat ccacattgat ctttgactta ttctcattat ctaaatgagc ataagtctta
      601 tcttcttcaa agcttatctt tattacctta ccctttattg ttcctttact cccctctttt
      661 tcaaccgttt ttccgattaa agaactattt tgatttattc tctgtacatc cattagctta
      721 ttgaagtttt gattcatgtt attcatctgt tctaatgaac taaactgtgc catctgggct
      781 ataaattctt tatcttccat cggttttaga ggatcctgat tctgcagctg agtaatcaac
      841 agcttaaaaa atgcatctct gtctagctcc tttttcacct gatttccaat tgcttgatca
      901 ttggcagctg cttgactgtt aactgcaccg gcagctccta taggcatatt acatctcctt
      961 tctttttaag caaagtattc gaatccactc ttatgggttt ctagagttcc ccaacctgct
     1021 ttttcaatct gttctacatt tccttcacta gattcgttgt ttcttaaaaa ttcatataca
     1081 ttgctctgtc ttttttggtt gttcttttca tgaccttctt taaactgctg aaagtaacta
     1141 ttagagtcct gattgttatg attatgatta aagtcttttg tttcagattc gatattaaat
     1201 tggtctatct gtaatccctt tgataggaga ttatttttca gttcctggag attgttttct
     1261 aaatagttct gaactatatt gttttctacc aatatcttac ctactatttt atttgattcc
     1321 atcgataagt ttattttcat cttaccgagt gaatcgggct ttaatttaat ctcgatttgc
     1381 tggttttgat tggacagatt ctttatatta actttatttt ttatttgatc taatacattg
     1441 ttttctttga ttaagttttt aaattgaata gtagaattct gttgatttgt attgttttta
     1501 atatcgaaac ttttcaatcc tgtgatattt tctttaccct gattattttg attttcacta
     1561 aaaaatttta gattattaac ttttttatat tttttgttag attccttact ttttatttca
     1621 aactctagat tattgaatgt aattttttct tttgtattat ctcgagtttt tacttgagaa
     1681 gtgtctttta gattatctcc cgcaactttt atatttttaa agttgcttgt tttattgtta
     1741 ggtttattgt gattaatttt tttattttta tttgtacttt cttgtttggc tttatctttt
     1801 gaaacttgct ctgtattaag gttagtattt ttaataatat tgtttgtttg gttttgtttg
     1861 tttttagaac tattattaat ttgttttact atattttctt ggatagtttt gatttcttta
     1921 ttcgtcaatt tgtttatttc tgtcaatatc ttttgtattt ttatattttc attaagttgg
     1981 gagttattag atacttttat tttgctgatt aatttgcttt gctgattagt ttttttattg
     2041 tctaatttgt ctgctagcat tttctttatc ttattaagta tattttcatc taattttttt
     2101 agactattta ggatatcttt tactgctttc tgtttatttt ctattaattc aatttcaggg
     2161 ttaaactcct tatcgttact tcttttaggt ttcttgtctg atttattatt ttgagctatg
     2221 ttatattttt ttaatttatt cagcagtttt tttaactttt cagcagattc tttattaact
     2281 tcatttgaat ttttaggaac ttcaattaaa atattgaatt tattttgtcc ttcaacttct
     2341 gttttactat ctgatttaat atctattagt gaaaattgtt tttgctcggt cggtatgttt
     2401 tcttgtctta ttattatctt ttgtatattt gctagatcaa tattttcttc ttttaagaac
     2461 tgtttgatct ctttaattag ttcatttgtt tttgtagatt gatttttttg atttaaattt
     2521 ctttttgttg ttttttggtt atttaaaatg gcttctattt tttctttgaa gttttccttt
     2581 aattcaactt gttttgaatc tgctttacca gtttttaact ggttggactg taaagtaact
     2641 gctaaagcat ttatttcact tcacctcctt ttttttgatt tgttatttat attaagagaa
     2701 cattgctatt cccttaatct ctctgttaat tgcaccgctt tatcctgcgg catattcatc
     2761 agtatttctg ccaagtgttc atcctttagt tcattaacta tggcaagggt tgtttccatt
     2821 ctcaagtctg ttattacatt agctgcttct tctgggtcca tattttcata gatctttacc
     2881 agtttttcaa ctctattttt ttcttcttct tggctttgtt ttaattcgga atatctgttt
     2941 tctaaggttt taattttttc gtttttttgt tcaattgtat ttttgacttg ttgaagttca
     3001 gactttaatt cgtcattagc tgtttttaat tcatcataag cattttctaa tttatagtag
     3061 tcattcatca tcctgtcata ctgtttatct gtcttcattc gctctttgat atagggtgtg
     3121 cttgtgacca catcatctaa ctgtaatatt ccaaatagat taagagccca tattatatat
     3181 actgtgagga agaggaataa aaagatatat attgcttttt taatcattct ataccccctg
     3241 ttgactcaaa cgaagactga gttcgtctag ttctttctgc tctttatcga ggaaatcttt
     3301 atagaaagct tcagatttct tttcctttat cttaacaatc ttttctttgg cctgtttttt
     3361 ctcgatgacc tgttcttggt attctttgac tacttctttt tgtctatgat atctattgga
     3421 tatttcttct atctttttct gctgtttttt aatatattct ctggtcctta tattttcagt
     3481 aactgtcaag ctattatatc ttatatagtc aaatagattg gatagttttt cttttgcatt
     3541 ttctaattct tcttttattt ctttttcttt ctgtcttttt tggtatagtt cattttgtgc
     3601 tttatctagt tcaacatccc ttatatccaa aactcgctgg agtctgaaat tgaatttagc
     3661 cataagatta cccctttaca ttatccattt taagaattta tgatctgtgc caatctattt
     3721 tttgtttctg aaaaacttac tttttcttcg atcctttgag ttagaaaatc attgattcta
     3781 tctattttat cgatagcccg gtccaccttc gggttactgc ccctttcata agcaccaata
     3841 ctgattaaat cttccgcttc tttatagtca gccagtagtt tctttaactg attagcattt
     3901 tcattatgtt ctgctgttac aacatctttc atgacacggc tgacgctttc taggacatca
     3961 acagccggga aatggttttt agatgctaaa ctcctcgata aggagatgtg gccgtctaaa
     4021 ataccccgta ctgtatcaga aattggttca ttgaagtcat caccttctac taagactgca
     4081 tacagggctg taattgaacc tttatcatta gtaccagttc tttccagtaa tttgggcagt
     4141 ttggcaaaaa ctgaaggggg ataacccctg gtagcaggtg gttcacctga agctaaacca
     4201 acttctctta aggccattgc cacccttgta accgagtcca tcatgagcag gacatcttta
     4261 ccctggtctc taaaaaactc agcgattgct gtcgctacac tggctgcttt cattctaatt
     4321 aaagccggtt tatcagaagt ggctacaact acaattgact tttttaagcc ttccggtccg
     4381 agatcttctt ctatgaagtc cctgacctct ctaccgcgtt ccccaattag ggcaataaca
     4441 ttaatgtcag cgtctgtatt acgggctgcc atcccgagca gggtactctt acctacccca
     4501 ctaccggcaa atatacctag ccgctggccg cggccgcagg ttaaaagacc gtctatactt
     4561 tttattccta aacttaaagg ttctttgatc cgctgtcttt ttaagggatc gggcggttct
     4621 ttatttacat tcacttcctt tagggttgct ttattaaatt cgtctttgtc tgatgaaagc
     4681 ggtcggccga gtccatccag gacttcaccg agaagttcat taccaatttc aactttaagt
     4741 tggctgccgc tggcaataac tttagcaccg ggtgtaattc ctttcatcac accgatcggc
     4801 atcagcagaa ctttgttatc attaaagccg acaacttctg cttcgatatc gccttcagtg
     4861 cttttaatat aacaaagttc tccaatacta acttctggtc cgactgactc tatgattagc
     4921 cctaccaccc tcgtgatata gccgaattcg cggttaatct tggtgttttc aattgtttct
     4981 tctaatttct ttaggttaag gtcatcaaac atttattagc tcatacccat ttctttttta
     5041 atttgctttt ctaatctggt cagtttttca gttaagtagg catctttacc gccgaaattt
     5101 gtatttataa cacagtctcc tttttgtaag gagtcatctg cattaaattc aaacttgctt
     5161 tccggaaaac tggttttaag ttcggtttta ctaagatagg gaagcatctc cggatttaca
     5221 ttaatcacaa actgctggtg gttattactt aa
//
LOCUS       DATGCJ010000236        16645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_170666, whole genome shotgun sequence.
ACCESSION   DATGCJ010000236 DATGCJ010000000
VERSION     DATGCJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 16645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16645
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170666"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VKN64_10430"
     CDS             <1..774
                     /locus_tag="VKN64_10430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HMA60701.1"
                     /translation="QAIDEKIKRSNLIEEKIQEKIEDEHILVDVEGKVPGQINGLSVY
                     QTGQYSFGRPTRITAQTFMGQEGVINIEREAEMSGKIHNKAVLIFSGYLGNQYAQDTP
                     LSLNASLAFEQSYGGIEGDSATAAELIAILSSISGYPIRQDLAITGSMNQKGKIQPIG
                     GVNEKIEGFYKVCKNKEITGNQGVILPEQNIDNLMLEEEVIEAIEDDKFHLYSISEID
                     EAIELMFDRPAEEVHAKVQEVMQEKAEKAAEFKGKGKEE"
     gene            787..1908
                     /gene="nagA"
                     /locus_tag="VKN64_10435"
     CDS             787..1908
                     /gene="nagA"
                     /locus_tag="VKN64_10435"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HMA60702.1"
                     /translation="MYIIKDVNIVTKDKVLKKHDIKFTDRIIDIKKEIASENKHQIID
                     GTNKFLSPGFVDIHLHGSFGIDIMNADQQDIVRMTDGLKKAGTTSFLPTVMTSSQERM
                     ENAIKTIKAVIDDGNQVIEGINLEGPLLNPSKAGAQDKRYMQALNKKMFEKYQDIIEI
                     VTVAPEVEGATVLINKLKKLGIVVSMGHTDAIYEESMAGFENGISLVTHLFNGMRGIH
                     HREIGAAGAALLTDVSVELIADGIHVSKPMIELILRLKDKNNIILITDAQPAALYEGD
                     EITFNDRKVILKEGSARYEDGTLAGSILSMHQAVKNMDKFSDLKIQEIIKMATFNPAQ
                     KIGKEDKIGSIELDKKADLLLLDKNLEIIEVFKEGEKVK"
     gene            1929..2666
                     /locus_tag="VKN64_10440"
     CDS             1929..2666
                     /locus_tag="VKN64_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMA60703.1"
                     /translation="MIKIEADLHVHSIFTGHAYSTIDELAEEAKNKGLKLIALTDHGP
                     ALPGGTHFNYFNNLKVLPDKIKGVKVLKGIEANILNDGSLDLEKRYFKHLDFVACGIH
                     MNTGYTNTTVKEHTKATINAIKNPLVDMITHPINLSNMVNIKEVIEAAKEHDVILEIN
                     ASSHKENMKGRGDKKLTRKLVKLAVDNGNYLALNSDAHYRDMVGDLSNLDPIINDGII
                     TNDDIINSSLDKVLTFLDNKQRKIEFD"
     gene            2825..3268
                     /locus_tag="VKN64_10445"
     CDS             2825..3268
                     /locus_tag="VKN64_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMA60704.1"
                     /translation="MFDLIPRRNRRSQNEMTERSEDPFDALWDSFFNNVMDRSKLGFR
                     TDVKEKDDKYIIDAELPGMNKEDIQVEMDDNYLTISANNEEYTEEEEEGYIRKERRKG
                     SYCRSFYIENIKEDEIEAEYNDGILEITLPKKEETVSKKKTIDIK"
     gene            3430..4272
                     /locus_tag="VKN64_10450"
     CDS             3430..4272
                     /locus_tag="VKN64_10450"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HMA60705.1"
                     /translation="MKEAAVIGAGTMGNGIAQVIADAGYKVILLDVNEEELKRGLETI
                     EQNLDRAIKKEKIKERDKNLILDRITTATEYEEIKDVDIVIEAVPEKMEIKKEVFKQL
                     DETLAEDVIIASNTSALSLTELATVTSRPEKVVGIHFFNPVTVMQLVELINNIITSED
                     TFNKARDFVDSLGKSAVEVNESPGFVVNRLLIPMINEAIFLVEENIASPDDIDTAMKL
                     GANHPLGPLALADLIGLDVCLSIMETLQSEFGDPKYRPAPLLKKKVRAGQLGRKTGEG
                     FYQY"
     gene            4290..5072
                     /locus_tag="VKN64_10455"
     CDS             4290..5072
                     /locus_tag="VKN64_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMA60706.1"
                     /translation="MDFKNIITKTEDKKAVIEINRPEALNALNIETLLEIEEAMHLIS
                     EDKNIRVIVITGTGDKAFIAGADIKEMKDMEPVEAESFANLGQRVFNKIENCRKPVIA
                     AVNGHALGGGCELALACDIRIASKNAQFGQPEVGLGILPGFGGTQRLGRIIGEGLARE
                     YIYTGRYISAERAEEIGLVNHLVDLDQLDVKVDEIAGEIINNSPCAISQAKKAINFSA
                     NAGKTEGMEYEAQLFGLCFSAQDQEEGMRAFINREKPEFEDK"
     gene            5104..5766
                     /locus_tag="VKN64_10460"
     CDS             5104..5766
                     /locus_tag="VKN64_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HMA60707.1"
                     /translation="MNKVVELNDLDGLFKDDLTIMIGGFLNCGTAETILNYVNQLGIK
                     NLTIIVNDAGFNQKSKGSFLANADIDKMIISHIGTNPDIGKKMNSGEIEVELVPQGNL
                     IEQIRCEGAGLGGVLTPIGIGTQAEEGNKVMEVDGKKYILATPLGADIAIIKAWQADE
                     SGNLIYRYSARNFNPIMAMAADTVIAEAEEVVKNGELENNNIETSNIFVDYIIESDKE
                     LL"
     gene            5800..6450
                     /locus_tag="VKN64_10465"
     CDS             5800..6450
                     /locus_tag="VKN64_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HMA60708.1"
                     /translation="MSEVKSKIASRIAEEVADGDVLNLGIGIPTLVCDYIPDDIEVNI
                     HSENGLIGLDREAEEGFEDKDIINASGRHATIKKTGSFFDTAMSFNIIRGGHLDMTVL
                     GALQVDQKGNIANWMIPGKLVPGMGGAMDLTVGAKRVVCAITHQTKKGASKIKKECNL
                     PLTAAGEVDLIVTELAVMEVDEKGLILKEISEGVTLEEVIDKTEADLIISDDLKEF"
     gene            complement(6494..7690)
                     /gene="ablA"
                     /locus_tag="VKN64_10470"
     CDS             complement(6494..7690)
                     /gene="ablA"
                     /locus_tag="VKN64_10470"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HMA60709.1"
                     /translation="MRNYKKYDLWKDVKEEEWNDWHWQVRNRITDVETLQQVIDINEK
                     EKEEIEGSLNTLRMAITPYYATLMDYSDRDCPIRKQAVPTSKELNMGNADMEDPLHED
                     VDSPVPGLTHRYPDRVLLLITDQCSMYCRHCTRRRFAGVTDKSMPMEKIDKAVEYIKS
                     DENIRDVLLSGGDALLVSDDKLEQIIKKIRAIEHVEIIRIGTRTPVVMPQRITENLVE
                     MLKKYHPIYLNLQFNHPKEITETAQNAIEKLNYAGIPVGNQSVLLKGINDQPAIMKKL
                     VQKLLYIRIKPYYLYQCDLSKGIEHFRTSVSKGIEIIESLRQHTSGLAVPTYVIDAPG
                     GGGKIPVSPQYMISQAENKVILRNYEGNIYAYTEPGYDKSKHEGDKVNRGVAKLIENG
                     YYTINE"
     gene            7840..8919
                     /locus_tag="VKN64_10475"
     CDS             7840..8919
                     /locus_tag="VKN64_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HMA60710.1"
                     /translation="MHKTPRANINLVNIKENARILKDECDKRGINITGVVKGSAGDLR
                     VINSVVEGGITTLGDSRIKNIIKMREAGFENEMVLLRLPRLSEIKQLIKYVDISLNSE
                     LVTLESIGRAALKKEKLHKVIIMVDVGDLREGVLVKDFMDFMEDALDIEGIKIIGLGT
                     NVGCYGGVLPTYDNTKILLDLKEQVKEKYGYDLEVVSGGNTATTILFEEDELPTGVNN
                     LRVGEGLIQGTDVTHQREIPFLNSNNITLTAEIIELKDKPSIPTGQIGHDAFGNKPKF
                     EDKGIRKRAILAVGRQDVKVEGMTPLIEGVEIIGASSDHLLCDVTDSRQDLQIGSEIE
                     FKLNYGAMLKAMTSEYVNKKYISSV"
     gene            complement(8916..10517)
                     /locus_tag="VKN64_10480"
     CDS             complement(8916..10517)
                     /locus_tag="VKN64_10480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMA60711.1"
                     /translation="MPNSKLFINGNVIDSKNTDAFLTTNGKIKETGQTKNLIGKYENY
                     AEVIDLNNKYVSPSFNDGHLHYFYYSLLKESIDLKNCSSINEVYSKIKNKADKTPADS
                     WITVINWDEEQFGGVEKLDRYVLDKLSKNIPILVKRRCLHLAFVNSKALDLANINKDT
                     PDPAGGKIVRDKDGFPTGELQDESIGIIDDIVLERQKKRFKSILKNSARDFLQNGITS
                     IQTDDLGHKKYREDIFKSYMELAKEDEIPLDITLQLRVSEKEDFDYYQKIRKETKDIK
                     GLKTGPVKFMFDGSLGGRTAALRKHYTDDTTTKGELLFNKEKLEELVDIAYQLDFQPA
                     IHAIGIKAVEIVSKIYEKINKKYSNKNLRPIIVHASMLDEKIMERVKKNNMVLSIQPT
                     FISSDYKMADKRLGKRRSQSLYRMRSLLEKGIKLAGSSDAPVEDINPLYGIYASVKRK
                     KPNKPQEKSWHPNEIIGIKKALQLYTTGPAYQNFQENSKGKIKEGYQADFITLNKNPL
                     TVNPEDLINLKIRSTYRNSDLVFEY"
     gene            10678..12150
                     /locus_tag="VKN64_10485"
     CDS             10678..12150
                     /locus_tag="VKN64_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMA60712.1"
                     /translation="MNTKWIKSEKSLNSESLCKMLNNLNEGIHVVDNKGTTILYNKKA
                     SEIDGLQPEEVIGKNLFEVFPSLTLETSTLMKTLKNEEKHLNHRQTFINYRGNKITTI
                     NHTFPIKFADDSKGAVEISKDITQTEELVDDITNLRNKLKDNSNSTESKKEVDNNTSF
                     EFADIIGQNPKFNSSVSDAMKCARSDSSVLIYGETGTGKELFAQSIHNASRRKNQEFI
                     AQNCAALPKDLLESLLFGTEKGSFTGAKSKPGLFEMADGGTILLDEINSMDLALQAKL
                     LRVLQDGRIRRIGGQKEKEVDVRIITTMNMHPDKAFEENKLREDLYYRLSVVYIYLPP
                     LRERKDDIMLLTKNFIKHYNQKFNKDIKGLEPELKKIFKNYSWPGNVRELKHVIESAY
                     NIKQYGDKIDIGDLPGYIIERLDHDRNINKDKNDFREHKVENTSELPSLDQYLEDIEL
                     NLIESALEKTNNNISAAARKLDISRQSLQYKIKKYNIKDN"
     gene            12161..13084
                     /locus_tag="VKN64_10490"
     CDS             12161..13084
                     /locus_tag="VKN64_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HMA60713.1"
                     /translation="MDITLILVFLIVGFIIGYFKPLPDIFYRFSGYITEIGLIILLGS
                     MGARIGVDEKIINKLGVIGLKSFTLALFSIIGSVLLLKIFSTIIKSNYQKNEQEKPLE
                     ESKSDHRLTIIILISIIIGIILGLTVLKSGYLPIIEVITTYALAALLFGVGVDIGLNK
                     EAFRELKILGFKIILIPILIILGTFLGTYIAGLFLNMVAQDALAVGAGFGWYSLSAVL
                     ITKMHSVELGSIAFLTNVFRELITIVSLPFIVRYIGKIPAIGPGGATTMDVTLPIVKK
                     FAGEETAIPAFISGVVLSTLVPILVPFILSL"
     gene            13136..14176
                     /locus_tag="VKN64_10495"
     CDS             13136..14176
                     /locus_tag="VKN64_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60714.1"
                     /translation="MDSKLSKLIINKNIKTISIIGLAKNTGKTVTLNQLIEELYLKGY
                     KLGLLSYGRDGESIDLITGQSKPPVKVYPGSFFITAERALNGAEIKYKKIKSTDISST
                     MGNVNLYQSLSEGVLQLVGINRLSQLIYVRDLLLNKVDYLLIDGALDRRSSALPKISG
                     AVVLSTGAVIGKDLNDVVDKTKYEVAKLKLPTLTNTDDLVTLQRFDQNKESVLWTDED
                     EIISFKTPTSLLLLNEIEDYLKESERGVKYIFINGAFTNTISRRIINLNKDDITVIIP
                     DSTKMFLDKMEYNKLVKNNIKLKVLYPLNLIALTINPFNPEGESLDDDKFLNNIAKNF
                     DIPVFNVKSKEY"
     gene            14190..15758
                     /locus_tag="VKN64_10500"
     CDS             14190..15758
                     /locus_tag="VKN64_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HMA60715.1"
                     /translation="MAIKLDLDQKLIDLCRTYTADIADDIEDKIAQNTTTSVERTVLR
                     LLGVDGVDEHGIPLPNVIVEKVQKEGKLHRGISYWFGNALLQTELEVQELAQAIAEDE
                     VDLFALAQAGNEKVYKHLLKLARAKADEIRENVKTRKEMKDEHPPGPKPWLYVIVATG
                     NIYEDVKQAKAAARQGADIIAVIRSTAQSLLDYVPYGPTTEGFGGTYATQTNFKIMRE
                     ALDEVSKEEGHYVQLVNYCSGLAMPEIAAMGAIERLDMMLNDAMYGILFRDINMKRTF
                     VDQHFSRMINAYAGVIINTGEDNYLTTADAVEEAHTVLASQFINEELGLRTGLSKEQL
                     GLGHAFEINPDIEDSFLMEIAQAQMVRQIFPDSPLKYMPPTKYMNGDIFRGQLMDGMF
                     NMASIMTGQSIQLLGMLTEAIHTPFIQDRALSIENAKHVFNSARNISDEIQFKPDGKI
                     QKRANQVLEETAEILHEIADKGLMKSLSEGIFADIKRPMDGGKGSDGVIEKSPDYFNP
                     FIDIFEEELDLDAG"
     gene            15748..16494
                     /locus_tag="VKN64_10505"
     CDS             15748..16494
                     /locus_tag="VKN64_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HMA60716.1"
                     /translation="MLDKDNLIKAYGDTYNDGKIQLSFTLPVQKGVEAEEAARVYCAK
                     IGLKEVEVVHMKDLKEGFTFFVVYAVADVFVDLNKINVAKIETEEMTYYEINDYIKEK
                     IGRELTVVGACTGSDAHTVGLDAILNMKGYAGEYGLERYPQFYTYNMGSQVPNEKLIE
                     KAVKNEADAILVSQVVTQKNVHIKNLTEFVEILEAEGLRDDFILVLGGPRISHELALE
                     LGFDAGFGSGTKPNQVASYIAQKVYQNIKK"
BASE COUNT         5645 a         2565 c         3472 g         4963 t
ORIGIN      
        1 caagcgatag atgaaaagat caaaagatct aacctaatcg aagaaaaaat tcaggaaaaa
       61 attgaagatg agcatatctt agtagatgta gaaggaaaag tacctggtca gattaatgga
      121 ctttccgttt accaaaccgg acaatattcc ttcggtcgac ctaccaggat aacagcccaa
      181 acctttatgg gtcaagaagg tgtaattaat attgaacggg aagctgagat gagtggtaag
      241 atccacaata aggcagtctt aatcttttct ggttatttgg gcaatcaata tgctcaggat
      301 accccactta gtttaaatgc ttctttagca tttgaacaaa gttatggtgg tattgaagga
      361 gattccgcca ccgcggccga gttaattgcg atactttcct caatttcagg ttatccgatc
      421 cgacaggatc tagcgattac aggttccatg aaccagaaag gtaagattca gccgataggt
      481 ggtgtaaatg aaaagattga aggtttctat aaagtctgca agaataaaga gataacaggc
      541 aatcagggag taattttacc tgaacaaaat atagataacc tcatgttaga agaagaagta
      601 attgaagcaa ttgaagatga taagttccac ctttactcaa taagtgaaat cgatgaagcg
      661 attgaattga tgttcgatag accagccgaa gaagtacatg ctaaagtcca agaagttatg
      721 caagaaaaag cagagaaagc tgctgagttt aaaggtaaag gtaaggaaga ataagaggtg
      781 ttttagttgt atatcattaa agatgtaaat atagtaacta aagataaagt actaaaaaaa
      841 catgatataa agtttacaga ccggattata gatatcaaaa aggaaatagc aagtgaaaat
      901 aaacaccaaa taatcgatgg aacaaataaa ttcttaagtc ctggttttgt ggatattcac
      961 ttacacggca gttttggcat agatatcatg aatgcagacc agcaagatat agtaagaatg
     1021 actgatggtc taaaaaaagc agggacaact tctttcctgc caacggtgat gacctcttcc
     1081 caagagagaa tggaaaatgc tataaaaaca attaaagcag taattgatga tggtaatcaa
     1141 gttatagagg gaattaactt ggaaggacca ttactgaatc caagtaaagc cggggctcaa
     1201 gataaaagat atatgcaggc tcttaataaa aaaatgttcg aaaagtatca agatataatt
     1261 gaaatagtga cagtagctcc tgaagttgag ggagctactg ttttaattaa caagttaaaa
     1321 aagcttggta ttgtcgtttc aatgggacat accgatgcaa tttatgaaga aagtatggcc
     1381 ggttttgaaa atggaatatc actggtaaca catctattta acgggatgag aggtattcat
     1441 caccgtgaaa taggagctgc cggagcagct ttacttacag atgtgagtgt agaattaatt
     1501 gctgacggta ttcatgtttc aaaacctatg atagaactta tcttaagatt aaaagataaa
     1561 aataatatta ttttaataac tgatgctcaa ccggccgccc tttatgaggg agatgaaatt
     1621 actttcaatg acaggaaagt aatattaaaa gaaggaagtg cccgttatga agatgggacc
     1681 ttagccggca gtattttatc gatgcatcag gctgttaaaa atatggataa atttagcgac
     1741 cttaaaatac aagaaataat caagatggca acttttaatc cagctcaaaa aattgggaaa
     1801 gaagataaga taggttctat tgagctagat aaaaaggctg acctattatt attagataaa
     1861 aatcttgaga taatagaagt ttttaaagaa ggtgaaaaag ttaaataata aattcaggag
     1921 tgaataaatt gataaaaatc gaagcagacc tgcatgtaca ctcgatattt actggacatg
     1981 cctattcaac tattgatgaa ttagcagaag aagccaaaaa taaaggactt aaattaattg
     2041 cactaaccga tcatggaccc gccttgccgg ggggaaccca ttttaattat tttaataatt
     2101 taaaggttct accagataag atcaaagggg ttaaggtatt aaaggggata gaagctaata
     2161 tcttaaatga tggttccctt gatcttgaaa agagatattt taaacatctc gattttgtag
     2221 cctgtggtat tcatatgaat acaggttata ctaatacaac ggtcaaagag catactaagg
     2281 ctacaattaa tgctatcaaa aaccctttag tagatatgat tacccatccc attaatctat
     2341 ctaatatggt taatataaag gaagtaatag aagccgccaa agaacatgat gtaatcttag
     2401 aaatcaatgc gagttcgcat aaagaaaaca tgaaggggcg gggagacaaa aaattaacga
     2461 gaaagttagt caaactagca gttgataatg gaaattattt agctttaaat agtgatgccc
     2521 attatcggga tatggttggt gatttaagta atttagaccc cattataaat gatggcatta
     2581 ttaccaatga tgacattatc aattccagcc ttgataaagt tcttacattt ttggataata
     2641 aacagagaaa aattgagttt gattaagata tagatattta attaatacca atcggcaata
     2701 agtgaaatta ttgccgattt tttagatttg aaatttatca tattttgatt ataataataa
     2761 gtagaaagtc aagaaaagtc agtaagtcaa ataaagtcaa aaataaaatt caggaggtgt
     2821 ctgtatgttt gatttaattc cacgtcgtaa ccgcaggagt caaaatgaaa tgaccgaaag
     2881 aagtgaagat ccttttgatg ccctttggga cagtttcttt aacaatgtga tggaccgttc
     2941 taagttaggg tttagaactg atgttaaaga aaaagatgat aagtatatta tagatgccga
     3001 actacctgga atgaataaag aagatatcca ggtagagatg gacgacaatt atctcaccat
     3061 ttctgccaac aatgaagaat acactgaaga agaagaggaa ggatatatta gaaaagaaag
     3121 aagaaaaggt agctactgca gaagcttcta cattgaaaat attaaagaag atgaaataga
     3181 agctgaatac aatgatggta tcctagaaat cacactcccc aaaaaagaag aaactgtaag
     3241 taaaaagaaa acaatagata tcaaataaat gaaagactaa ctataatccc ggtttaaccg
     3301 ggatttttta tttttttata ataaaagtta aaaaatgcac ataatatgga acgatttttg
     3361 cataatatat atgaatagaa taaaattaaa ctataatagt ttgtataata agtaaagggg
     3421 gagttttaag tgaaagaagc agcagtaatt ggtgctggca ccatgggtaa tggtatagcc
     3481 caggtaatag cagatgcagg ttataaggtt atacttttag atgttaatga ggaagaatta
     3541 aagaggggat tagaaactat tgaacaaaat ttagatcgag cgatcaaaaa agagaaaatc
     3601 aaagaaagag acaagaactt aatcttagat agaattacca ctgctacaga atatgaagag
     3661 atcaaagatg ttgatattgt aatagaagct gtacctgaaa agatggaaat caaaaaggaa
     3721 gtttttaaac agttagatga aacactggct gaagatgtaa tcattgcaag taatacttcg
     3781 gccctttcac tgactgaatt ggcaactgta accagtcggc cggagaaagt ggtaggtatt
     3841 cacttcttca atccggtaac cgtgatgcag ttggtagagt taattaacaa tataataaca
     3901 agtgaagata cctttaataa ggcccgtgat tttgttgatt ccttaggtaa gtcagcggtg
     3961 gaagtaaatg aatcacccgg ctttgtggta aatcgtcttt taattccgat gattaatgaa
     4021 gcgatctttt tagtagagga aaatatcgcc tctccagatg atattgatac cgctatgaaa
     4081 ttaggggcaa accatccttt aggacccctc gctttagcag atttaatcgg tttagatgtc
     4141 tgtctttcca ttatggaaac cctccagagt gagttcggag atcccaaata taggcctgcc
     4201 cctttgctca agaaaaaggt tagggctggc cagttaggta gaaaaaccgg agaaggattt
     4261 tatcaatact aagagagaag ggataacaaa tggattttaa aaatattatt accaaaaccg
     4321 aagacaaaaa agctgtgata gaaattaacc gccctgaggc tctcaatgct ttgaatattg
     4381 aaacattatt agagattgag gaagctatgc accttatttc tgaggataaa aatattcggg
     4441 tcattgtaat aaccgggacc ggagataaag cctttattgc cggggcagat attaaagaga
     4501 tgaaagacat ggaacctgta gaagccgaat cttttgcaaa tctgggccag cgggttttta
     4561 ataagattga gaactgcaga aagcccgtaa ttgcagctgt caatggtcat gctttaggtg
     4621 ggggttgtga attagcctta gcctgtgata ttagaattgc cagtaaaaat gcccagtttg
     4681 gtcagccgga agtagggctc gggatattac cgggctttgg cggtacacag cgactgggta
     4741 gaatcattgg ggaaggcctg gctagagagt atatatatac cggccgatat atcagtgctg
     4801 agcgtgcaga agagattggc cttgttaacc atttagttga tttagatcaa ttagatgtaa
     4861 aggtagatga aattgccggg gaaataatta ataattcacc ctgtgcaatc agtcaggcca
     4921 agaaagcaat aaactttagt gctaatgccg gtaaaacgga aggaatggaa tatgaggccc
     4981 agttatttgg gctctgtttt agtgcccaag accaggagga aggaatgcgg gcctttataa
     5041 acagagaaaa accagaattc gaagataaat aaaaattgac tttatcttac aaggaggctg
     5101 tcaatgaata aagttgttga actaaatgat ttagatggtt tatttaaaga tgaccttact
     5161 attatgatcg gtggattttt gaactgcggc acagctgaaa ctattttaaa ttatgtaaat
     5221 caactgggta ttaaaaattt aacaataatt gttaatgacg caggctttaa tcagaaaagt
     5281 aaaggtagtt ttttagcaaa tgcagatatc gataagatga taatttcaca catcgggaca
     5341 aatccggata ttggtaaaaa gatgaatagt ggagaaatag aagtagaatt agttccccag
     5401 ggtaatttaa ttgaacaaat cagatgtgaa ggagccggtt taggaggagt attaactccc
     5461 atcgggatag ggactcaggc tgaagaaggt aataaagtaa tggaagtaga tggcaagaag
     5521 tatatactgg ctactccctt aggagctgat atagcaatta ttaaggcctg gcaggctgat
     5581 gaaagcggca atttgatcta taggtattct gcccgtaact ttaatccgat tatggctatg
     5641 gcggcagata cggtgatagc tgaagcggaa gaggttgtta aaaatggtga gctagagaac
     5701 aataacatag agacttccaa tatttttgtt gactatatta tagagagtga caaagagtta
     5761 ttataattat ctattaaaag tgatggaaag ggtgagttga tgtccgaggt taaaagcaag
     5821 atagccagcc ggatagcaga agaggttgca gatggtgatg tattgaattt aggtattggg
     5881 attcccactt tagtttgtga ctacatacct gatgatattg aagtaaatat ccattctgaa
     5941 aatggtctaa taggtcttga tagggaagct gaagaaggat ttgaagataa agatattata
     6001 aatgcaagcg gccggcatgc tactattaaa aaaacaggca gtttctttga taccgcgatg
     6061 tcctttaata taattcgcgg tggtcatttg gacatgaccg tcttgggggc acttcaggta
     6121 gaccaaaaag gtaatattgc gaactggatg atccccggca aattagttcc cggtatgggt
     6181 ggggctatgg acttaacagt gggagcaaaa agagttgttt gtgccataac ccatcagact
     6241 aaaaagggag catctaagat aaaaaaagag tgtaatctac ctttaactgc agccggtgag
     6301 gttgatttaa tagttaccga actggcggta atggaagtcg atgaaaaagg cttaatttta
     6361 aaagagatat cggaaggggt aactttagag gaagtaattg ataagactga agcagatttg
     6421 atcattagtg atgatttaaa agagttttaa ttagaaaagc aaaagggcac cgtgaggtgc
     6481 ccttaaaata tttctattca tttatcgtat aatagccgtt ttcaattagt ttggctactc
     6541 cccgatttac tttatctccc tcatgtttag acttatcata gcctggttct gtataggcat
     6601 aaatattacc ttcataattt cttaggataa ccttgttttc agcttgagag atcatatact
     6661 ggggactgac cgggatttta cctcccccac cgggtgcatc tattacatat gttggtaccg
     6721 ccaggccgga agtgtgctgt cttaatgact caataatctc tatacccttg ctaacagaag
     6781 tcctgaaatg ttcaatacct ttagaaagat cacactggta taagtaatag ggtttgattc
     6841 taatatataa aagcttttgt actagttttt tcattatggc aggttgatca ttaatacctt
     6901 tgagtaagac agactgattg ccgaccggta ttccggcata gtttaacttt tcaatcgcat
     6961 tttgggctgt ctcagttatt tcttttggat gattaaactg taaatttaga tagatcgggt
     7021 ggtatttttt taacatttca actagatttt ccgtaatcct ctgcggcatt acaaccggtg
     7081 ttctggtccc gattcttata atctctacgt gctctattgc ccttatcttt tttataattt
     7141 gttctaattt atcatcagaa actaatagag catctccccc ggataaaagt acatctctta
     7201 tgttttcatc acttttaata tattctaccg ctttatcgat cttttccatc ggcattgact
     7261 tatcggtaac accggcaaac cttcttctcg tacagtgacg gcagtacatt gagcactggt
     7321 ctgtaattaa aagcagtact ctatcgggat agcggtgggt aagtcccggt accggtgaat
     7381 ctacatcttc atgtaaaggg tcttccatgt cggcatttcc catattcaat tctttagaag
     7441 tggggacggc ctgtttgcgg ataggacagt ctctatcact ataatccatt aaagttgcat
     7501 aataaggggt gatagccatc ctgagggtat ttaaagagcc ctcaatttct tctttttctt
     7561 tctcatttat atcaattact tgttgtaagg tttctacatc ggtaatcctg ttgcgaacct
     7621 gccagtgcca atcgttccac tcctcttctt ttacatcttt ccataaatca tactttttat
     7681 aatttcgcat tgtctacctc ctataaagca aaaatatatt aataccacca tatttaatat
     7741 gcaataatca ttccagttga ctttgctgtt gaagataaat atataatgta atataattaa
     7801 aattgttgat aaaaatatta ttagttagga agtggtttta tgcataaaac tccgcgtgcc
     7861 aatattaatt tggttaacat aaaagaaaat gccagaattt taaaagatga atgtgataaa
     7921 agagggataa atataaccgg tgtggtgaag ggaagtgccg gtgatttaag ggtcatcaat
     7981 tcagtagtag aaggcggtat tactaccctg ggagattccc gtataaaaaa tatcatcaaa
     8041 atgagagaag ccggttttga aaatgaaatg gttctgctta gactgccccg tttatctgag
     8101 ataaagcagt taattaagta tgtagatata agtttaaatt ctgaactagt caccttagaa
     8161 agcatcggga gagccgccct aaaaaaagaa aagttacata aggttattat tatggtagat
     8221 gtaggtgatt taagagaagg tgtactcgta aaggatttta tggacttcat ggaagatgcc
     8281 cttgatattg aagggattaa gataatcggt ttaggcacca atgtaggctg ttatggtggg
     8341 gtcttaccta cttatgacaa tactaaaata ttacttgatt taaaagagca ggttaaagaa
     8401 aagtatggtt atgacctgga ggtagtatcg ggtggtaata cagcgaccac catcttattt
     8461 gaggaagatg aactaccgac cggtgtcaat aatttaagag taggagaagg tttaattcag
     8521 ggtacagatg taacccatca aagagagatt ccatttttaa acagtaataa tataacctta
     8581 acggctgaaa taatagagtt aaaggataaa ccttctatac ctaccggtca gatcggtcat
     8641 gatgcctttg gcaacaaacc gaaatttgaa gataagggaa ttagaaaaag ggccatcctg
     8701 gcagtgggca gacaggatgt taaagtggaa gggatgacac ctctaataga aggggttgaa
     8761 ataataggag ccagtagtga ccacttatta tgtgatgtta ctgactcccg tcaagattta
     8821 cagattggta gtgagataga atttaagtta aattatggag ccatgcttaa agcaatgact
     8881 tctgaatatg ttaataaaaa atatattagt tcagtttaat attcaaatac taaatctgaa
     8941 tttctgtaag tacttcttat tttaagatta attagatctt ctggattaac agtaagggga
     9001 tttttattta aagtaatgaa atcagcttga taaccttctt taattttacc cttactattt
     9061 tcttggaaat tttgataagc aggccctgtg gtataaagtt gcagggcttt tttaattcca
     9121 ataatttcat tgggatgcca gcttttttcc tgaggtttat taggtttttt tcttttgaca
     9181 gaggcatata tgccgtaaag aggattgata tcttcaaccg gggcatcaga gcttccggcc
     9241 agtttaatgc ccttttccaa taggctccgc attctataaa ggctttgtga ccttcttttg
     9301 cccagtcttt tatctgccat tttatagtca gaactgataa atgtaggctg gatagaaagt
     9361 accatattat tctttttaac ccgttccata attttctcat cgagcattga agcatgtaca
     9421 ataatcggtc ttaggttttt gttagaatat tttttattaa ttttttcata aatcttactc
     9481 acaatttcaa cggctttaat tcctatagca tggatggccg gttgaaaatc caactgataa
     9541 gctatatcta ctagttcttc cagcttctct ttattaaata agagctctcc tttggtagta
     9601 gtatcatctg tgtaatgttt tctaagggcc gccgttctgc cccccaaaga gccgtcgaac
     9661 ataaatttaa caggcccggt tttaagtccc ttaatatcct tggtttcttt tcttattttt
     9721 tgataataat cgaaatcttc tttttcagat actcttagct gtaaagtaat atccagcggt
     9781 atttcatctt cttttgctaa ctccatataa cttttaaaaa tatcttctcg atatttttta
     9841 tggcccagat cgtcggtttg gatagaggtt ataccatttt gtaaaaaatc ccgggcggag
     9901 ttttttaaga tacttttaaa tctttttttc tgtctttcca ggacgatatc atcgattata
     9961 ccgatcgact catcctgcag ctcaccggta ggaaaaccat ccttgtccct aactatttta
    10021 cctccagctg gatcgggtgt atctttattt atattagcca gatctaaggc tttggaattg
    10081 acaaaggcca gatgtaggca ccggcgtttt accaaaatag gaatattttt agatagttta
    10141 tctaatacgt atctatctaa tttttcgaca ccgccaaact gttcttcatc ccagttgata
    10201 actgtaatcc agctatctgc gggtgtctta tctgctttat ttttgatttt actgtagact
    10261 tcattgatag aactgcagtt ttttaagtcg atactttctt ttagcaagga atagtaaaaa
    10321 taatggagat gtccatcatt aaaagatggt gatacatatt tgttattgag gtcaatcact
    10381 tctgcatagt tttcatactt gcctattaaa ttttttgtct gaccggtttc tttaattttc
    10441 ccattagttg ttaaaaaagc atctgtattt ttactgtcaa ttacattacc attgataaaa
    10501 agttttgaat ttggcatttt ctcacctcac tatattatct tccacaaagc agatatttat
    10561 tccttttttg cgcaagaaat ttttctaata tgcaaaaaaa cttgcattaa aaggcaggtt
    10621 taaattattt taagtagaat agttaagata agcgaaaaaa atctcagggg gatatgtatg
    10681 aacacaaaat ggattaaatc agaaaaaagt ttaaatagtg aaagcctttg taagatgtta
    10741 aataatttaa atgaggggat ccatgtagta gataataagg gtactactat cttatacaat
    10801 aaaaaggctt cggaaattga tggattacag ccggaagaag taattgggaa aaacctgttt
    10861 gaagtttttc cttcattaac cttggagacc tcaactttga tgaagacttt aaaaaatgaa
    10921 gaaaaacatt tgaaccatcg acaaactttc ataaattata ggggtaataa aattacgact
    10981 atcaaccata cctttcctat taaatttgcg gatgacagca aaggagctgt tgagatatcg
    11041 aaggatatta cccagaccga agagttggtt gatgatatca ctaatttaag gaacaaatta
    11101 aaagacaaca gtaattccac tgaaagtaaa aaagaggttg ataataatac cagttttgag
    11161 tttgccgata ttatcggcca gaaccccaag tttaacagtt cggttagtga tgctatgaaa
    11221 tgtgcaagaa gtgattcatc tgtcctaatc tatggtgaga cagggacagg taaggagctt
    11281 tttgcccaga gtatacataa tgcttcccga cggaaaaacc aggaatttat agcacaaaac
    11341 tgtgctgctt taccgaaaga cttgttagaa tctcttcttt ttgggaccga aaaaggtagt
    11401 tttaccgggg caaaaagcaa gcccggtctt tttgagatgg ccgatggcgg gactatttta
    11461 cttgatgaaa ttaactctat ggacctggct ttacaagcta agcttttaag ggtcctgcag
    11521 gacggtagga tcagaagaat cgggggccaa aaagaaaagg aagttgatgt aaggattatt
    11581 acaaccatga acatgcaccc cgacaaggct tttgaagaaa acaagttaag ggaagacctc
    11641 tattacagat taagtgtagt atatatttat ctaccccctt tacgggaaag aaaagatgat
    11701 ataatgcttt taacaaagaa ctttattaaa cattataatc agaaattcaa taaagatatc
    11761 aagggtttag aacccgaatt gaaaaagatc tttaaaaatt acagttggcc gggcaatgta
    11821 agagaattaa aacatgtaat cgaaagcgcc tataatataa aacagtatgg agataagatc
    11881 gatatcgggg atctacctgg ttatataatt gaaagattag accatgatcg taatataaat
    11941 aaagacaaga atgactttag agaacataaa gttgaaaata caagtgaatt accttcttta
    12001 gaccagtatt tagaagatat tgagttaaac ctaatagaaa gtgctttaga aaagacaaat
    12061 aataatataa gtgctgcagc ccgcaagttg gatatttcca ggcagtccct gcagtataag
    12121 ataaaaaaat ataatataaa ggataactag gagtgaggat atggatatta cattaatttt
    12181 agtattttta attgtcggtt ttatcatagg atactttaaa cctctgccag atatttttta
    12241 tagattttca ggttatatca cagaaatcgg tttaattata ttgctcggat cgatgggagc
    12301 ccgtattggt gtagatgaaa agattatcaa taagctgggg gttattggac ttaaatcatt
    12361 tacgctagct ttatttagca tcatcgggag tgttttatta ttaaaaatat tttccactat
    12421 aattaaaagt aattatcaaa aaaacgagca ggaaaagccg ctggaagaga gtaaatctga
    12481 ccacagactg accataataa tcttaatatc gattattatc ggtatcattt taggcttaac
    12541 tgtactaaaa tcaggctact taccgatcat agaagtaatt accacttatg ccctggctgc
    12601 tttattattc ggggtaggag tggatattgg attaaataaa gaggcctttc gggaactaaa
    12661 gattttaggt tttaagataa tattgattcc tattttaatt atattaggga cttttttagg
    12721 gacttatatc gcagggctct ttttaaatat ggtagcccag gatgctctgg cggtaggggc
    12781 tggattcggg tggtatagct tgtcagcagt gctgattacc aagatgcaca gtgtagaact
    12841 gggttccatt gcctttttaa caaatgtatt tagggaatta attaccatag tttcactgcc
    12901 ctttattgtc aggtatattg gtaagatccc ggccattggt ccggggggag ctaccactat
    12961 ggatgtaacc cttcctattg taaaaaaatt cgcgggtgaa gagaccgcca ttcccgcatt
    13021 tattagtggt gtagtacttt ccaccctggt tcctattttg gttccattta ttttaagtct
    13081 ttaataatat tattatggca taataattgc attttcttta aaaggggcaa aaagaatgga
    13141 ttctaaatta agtaaattaa taattaataa aaatattaaa accatatcta ttattggttt
    13201 agctaaaaat acgggtaaga ccgtaacctt aaatcagctt attgaagaac tttatttaaa
    13261 aggatataag ctgggacttt tatcatatgg tcgggatgga gagagcattg atttaattac
    13321 cgggcagtcc aaacctcccg ttaaggtcta tcccggtagt ttttttataa cggctgagag
    13381 agccctaaat ggggctgaaa taaagtataa aaagattaaa agtactgata tttcttcaac
    13441 gatgggtaat gtcaacctct atcagtcttt aagtgaaggg gtactccagc tggtggggat
    13501 taaccgttta agccagttaa tttatgtgag ggaccttctg cttaacaagg ttgattacct
    13561 tttaattgat ggggctttag accgcaggag ttcagccctg cctaaaataa gtggagcagt
    13621 tgttttatca accggggctg taataggtaa agatctaaat gatgtagttg ataagacgaa
    13681 gtatgaagta gcaaaattga aactgcccac tttaaccaat acggatgacc tggttacttt
    13741 acagagattt gatcaaaata aggaaagtgt cttatggact gatgaagatg aaataatcag
    13801 ttttaaaaca cccacttccc ttttgctttt aaatgaaatt gaagactatt taaaagaaag
    13861 tgaaagaggg gttaaataca tatttatcaa cggagctttt accaatacca tcagccgccg
    13921 gattattaat ttaaataagg atgatatcac agttattatc cctgattcga ctaaaatgtt
    13981 tctagataag atggagtata acaagttagt gaaaaataat attaagctta aggttcttta
    14041 cccacttaac ctgatcgctt taacgatcaa tccttttaat ccagaaggtg aaagtttaga
    14101 tgatgataag tttttaaata atatagcaaa aaactttgat attcctgttt ttaatgtaaa
    14161 atccaaggaa tattgaaagg aggagtacct tggcaataaa actagattta gatcaaaagt
    14221 taatcgatct ttgtagaact tatacagctg acattgcaga tgacattgaa gacaagatag
    14281 cccaaaatac cacaacctca gttgagagga ctgttttaag attactggga gtagatggag
    14341 tagacgagca tggtatacct cttcccaatg taattgttga aaaggtacaa aaagaaggaa
    14401 aattacacag aggtatttct tactggtttg gcaatgcatt attacagacc gaactagagg
    14461 tgcaagaact ggctcaggcg attgctgaag atgaagtaga tctttttgca cttgcccagg
    14521 caggtaatga aaaggtctac aagcacttat taaaactggc cagagccaaa gcagatgaga
    14581 tcagagaaaa tgtaaaaaca agaaaagaga tgaaagatga acatccgccg ggacctaaac
    14641 cctggcttta tgtgattgtg gccaccggta atatttatga agatgtaaaa caggccaagg
    14701 cagccgcccg tcagggggcc gatattattg ctgttattcg cagtactgcc caaagccttt
    14761 tggactatgt accctatggt cccaccacag aaggatttgg aggtacctat gcgacccaga
    14821 caaactttaa gataatgagg gaagccctgg atgaagttag taaagaagaa gggcattacg
    14881 tacagttagt caattactgt tctggtttag ctatgcccga aattgcggcc atgggtgcaa
    14941 tcgagagact ggacatgatg ttaaatgatg ccatgtacgg catcttgttt agagacatca
    15001 atatgaagcg tacctttgtg gatcagcatt tttcccgcat gatcaatgct tatgctgggg
    15061 tgataataaa tacgggtgaa gataattatc tgactactgc cgatgcagta gaagaggccc
    15121 atacagtttt agcttcccag tttatcaacg aagagttagg tctaagaact gggttatcga
    15181 aagaacagct cggtttaggg catgcctttg agattaatcc cgatattgaa gacagctttt
    15241 tgatggagat tgcgcaggct cagatggtta ggcagatctt tccggattca cccttaaaat
    15301 atatgccgcc gacgaagtat atgaacggcg atatatttag aggtcaattg atggatggta
    15361 tgttcaatat ggcttcaatt atgaccggcc agagtattca actgttaggt atgttaacag
    15421 aagcaataca tacacctttc attcaggatc gggccttaag tatcgaaaat gctaaacatg
    15481 tatttaattc ggctcgtaat atcagtgatg aaattcagtt taaacctgac ggcaagatcc
    15541 aaaaaagggc caaccaggtt ttagaagaaa cagccgagat tctacatgaa attgctgata
    15601 aaggactgat gaagtcctta tcagaaggta tctttgctga tatcaagcgc ccaatggacg
    15661 gtggtaaggg atctgatggt gtaattgaaa aatcccccga ctactttaat cctttcattg
    15721 atatattcga agaggagttg gatttagatg ctggataaag ataacctaat aaaagcatat
    15781 ggagatacct ataatgacgg caaaatacag cttagtttta ccctacccgt acaaaaagga
    15841 gtagaagctg aagaagcagc ccgggtctat tgtgcaaaga tcggcctaaa agaggtagaa
    15901 gtagtgcata tgaaagatct taaagaggga tttacctttt ttgttgttta tgcagtggct
    15961 gatgtttttg ttgatctgaa taagatcaat gtagccaaga ttgaaacaga agagatgacc
    16021 tattatgaga ttaatgacta tataaaagaa aagataggga gggagcttac agtagtaggg
    16081 gcctgtaccg gttcagatgc ccatacagtc ggattagatg ctatcctaaa catgaagggt
    16141 tatgccggtg aatacggcct ggagagatac cctcagttct atacatataa tatgggcagc
    16201 caggttccca acgaaaagtt gattgaaaag gctgttaaaa atgaagctga tgcaatctta
    16261 gtttcccagg tagttaccca gaagaatgtt catataaaaa atttaaccga gtttgtagag
    16321 atcttagaag cagaaggttt aagagatgac tttattttag tattaggcgg tccgcgtatc
    16381 agccatgaac tggctttaga attaggtttt gatgctggat ttggttcggg tacaaaacca
    16441 aatcaggtag catcctatat agcacaaaaa gtttatcaaa atatcaagaa ataattagtg
    16501 aggtggaaag attggaaaca gaagtaatga taagaatgag aatgagtaca aatgatgccc
    16561 attatgccgg caatttagtc gatggtgcca agatgttaga actctttggt gatgttgcaa
    16621 ctgagttatt aattagaagt gacgg
//
LOCUS       DATGCJ010000237         6177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_395476, whole genome shotgun sequence.
ACCESSION   DATGCJ010000237 DATGCJ010000000
VERSION     DATGCJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395476"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VKN64_10510"
     CDS             complement(<1..718)
                     /locus_tag="VKN64_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMA60717.1"
                     /translation="MNKKFFLFRYLFLIFLLSTLILILLAGCSNNGTDLPKISNYNVI
                     FTVLDESSQPIGGAEFTLDETTETTDSEGIVAYTKPDGTYNYTVSADNYNSKSDTVTV
                     DGNDISQSVSLNMIEYDITFTVTDTADTSIEGANVSLDGVNKTTDSSGIVTYTKPDGT
                     YNYTVSADNYDSKSDTVAVDGDNISESVSLNMIEYDITFTVTDTADTAIEGASVSLDG
                     VTKTTDSSGIVTYTKPDGTYN"
     gene            1550..1951
                     /locus_tag="VKN64_10515"
     CDS             1550..1951
                     /locus_tag="VKN64_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60718.1"
                     /translation="MLGEQTQDDDFANIDPQDGKDLKSDVLQNPSDPDAIYRFKYDNN
                     IGYTDNVVEAFDGENNIIKNYDLEPNTYSNQKFSRDTIKTLSKTTERKKILKMLIGGT
                     YFSIELAKEAIKEGIKLIPGELTGRKPDKNK"
     gene            complement(3308..>6177)
                     /locus_tag="VKN64_10520"
     CDS             complement(3308..>6177)
                     /locus_tag="VKN64_10520"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMA60719.1"
                     /translation="DNYVLQAKADTFSGREISWVGFIYKIDKSQYSSWNDFKVDWESN
                     PHRADIQVESNRPVGKIYLGADSVFGYIDLLSVGIDYIVDKIFENDNIFDNDNIPGEN
                     KYRFSEEEGGYALHLYTMGAGLHNYENKKKDMELIRNWPYSKWGLATACSVNALEISL
                     KSVSLSFAVMDYVEPQYYTEELQEICESFVQNFEAGVSIKTVLSTNHVFNKAVMQLSK
                     RISKQVANKIVKKGLKSILKFAGKQFVKAVDIPGMIATSGELGTRIGTAAGITPWEGS
                     IGAVNPLTCILKSPSNGVADVDTNLILSWGVSTAPEVYSVTYDVYLGTSSALFTKVAD
                     SISSTSYEVSGLSQNTTYYWKVVAKDGKGGISESDVWEFTTIDGPTLTIEKEGEGIIL
                     VNDMEFSLPYKNGFKEGDIVNLETLTSDDWKFKEWTGDISSTNPTTTITMNEDKNITV
                     VFEEENNPPIIDISSSTDNLTATISGPITDSDGTVEEVTIDWGDENTTTISSGFDSIN
                     ESHIYASSGSYTVKVSATDSKGATASESLTVNVSETNNTPIIDVSSSTNNLTATISGS
                     ISDSDGNVSSAEIDWGDGNTTTISNAFDSIDENHTYNSEGTYDVIITATDDDGKTANT
                     SLKVPVEEKFPLFNITIINYPSVVDIGVEMNIEVEVTNNGDEFGEAFIDLFIDSTGSF
                     VDDKEVSLVPGESEIVELSYITEEDDGSEIEVIVKPFNKTTNEYDSIDKVWVDLEVLN
                     QKPYFEVDIIDYPLEPVYYEEEITVRGEVKNTGGAGSQDILFSFGKYHAEAKENIELD
                     PGERIEISFNHEPIEDDIGYNTISLASVDSEIIDQDILEDKIEVIGLLFTDLEPENDP
                     IYVTEPDYHAKLYCSGSNFENVQAIVYEWKGAVSGSSYWIKGDNDWNLAVTIESDTSM
                     ILEPIVIENEPSWAGTLYYTVTLMDDRGQTASQSFEVTYEP"
BASE COUNT         2090 a         1081 c          867 g         2139 t
ORIGIN      
        1 aattatatgt accatctggt ttagtataag tgactatacc gctgctatca gtagttttag
       61 ttacaccatc gagactaaca cttgctcctt caatagctgt atcggcagta tctgttacag
      121 taaatgtgat atcgtattca atcatattta atgaaacact ttcagagata ttatcaccgt
      181 ccacggctac tgtatcagat ttagagtcat aattgtctgc gctaacagtg taattatatg
      241 taccatctgg tttagtataa gtgactatac cgctgctatc agtagtttta tttactccat
      301 ctagagaaac atttgctcct tcaatagatg tatcggcagt atctgttaca gtaaatgtaa
      361 tatcgtattc aatcatattt aatgaaacac tttgagagat atcattacca tctacagtaa
      421 ctgtatcaga tttagagtta taattgtctg cgctaacagt gtaattatat gtaccatctg
      481 gtttagtata ggcaactata ccctcactat cagtagtttc tgtagtttcg tcaagtgtaa
      541 attctgcacc ccctattggt tgactacttt catctaatac tgtaaaaatt acattatagt
      601 ttgatatctt aggtaaatct gttccattat tagaacatcc agcaagtaaa attaatatta
      661 atgtagataa tagaaaaatc aaaaataagt atctaaataa aaaaaatttt ttgttcattt
      721 ttaccatcct ttatatattg tatttgtacg attagcgcag aaaaaataaa tattatatat
      781 ataatatttc aacatatatg ggaaaaatcc tttaataatt ttataatttt cagaatattt
      841 tttattttgg tagtatggtg ttagcctcca aaaagattaa gtatatttct tatataacta
      901 aataatacca atgtaatgcc ctttacaaat tactttaatc atccttaagt tagttaaaca
      961 aattcccgat aatctattat atgacagaca ttatgtgata taatataatt aaggataaaa
     1021 agtgaaaaga ttatataatg tttgtaaaaa atagtcacca ccaattaagt ttatacgact
     1081 cttttgatga tatgcctaaa catatccagg aatatccgcg tagctgatgg ttcatacttt
     1141 tcaagaaatt atttttccag ctattaatga agaacgtttt tttgttttat acagtgataa
     1201 agggtccaaa ctaaatactc ttgttaatat aattatttgc agccttatta acaaagaact
     1261 ttttgagctc actgatgaaa gtctggatgc taatattcat ctaaatatgg agtttcaata
     1321 tactttaatg ttaactagta aaaaaagact acccatctat aaagatacat tttataattt
     1381 tagagaacgg gttaaaaagt aatgtcaaga aaataatata gatttaatac aacaagaaat
     1441 ttaagtttta gcgaatataa ttgcaaatta cttagaaatc gaatgcaata aagtagttaa
     1501 tactggtggt aaagttattg aaactgaaga attcctttta ctaaaaagaa tgctcggtga
     1561 gcagactcaa gatgatgatt ttgctaatat tgatccgcaa gatggtaagg atttaaaatc
     1621 tgatgtttta caaaatccca gtgatcctga tgccatttac cggtttaaat atgataataa
     1681 tatcggatac actgataatg tagtagaagc ttttgatggc gaaaacaata ttattaagaa
     1741 ttatgatcta gagcccaata catatagtaa tcaaaaattt agtagggata ctattaaaac
     1801 actttctaaa acaacagaaa gaaaaaaaat tttaaagatg ttaataggcg gaacatattt
     1861 ttctatagaa ctagctaaag aagctataaa agaaggtatc aagttaatcc ctggtgaatt
     1921 aactgggaga aagccagata aaaacaaatg aaattattcc aataatttca agggcaatga
     1981 tcaaaatata attacaggtt gtgctgaagg ccataaatct ttttacagtg actatgatga
     2041 tgaaaatgag gtatttactg ctaagttttt aaaagaaaac tgtaaaaact gcaacctcaa
     2101 agaaaaatgt cggatcaact ttcaaaagaa agcaaacact attaggttta acaaaagaca
     2161 atatgataca gataagttcc gtaaaaagat ggatactaaa cagtatcaag aacttgccaa
     2221 ccagagagcg gggatagagg gattatcttc tgtatttaga aaataataca atgttgatta
     2281 tatgcccgtc atgggattag tgcgttcaaa attctacttt tcattcaaag tattagcaag
     2341 taatgttaaa aagttgttca aaagaccaaa agtagttact ggataaggac tatttttatc
     2401 tatatttaat ttttaactat tttaattgat attttctagt ttttccgtta tatagagtca
     2461 tttatttagg ttgtttttgt gttggaacca tattatttca tataaaaaat attttcctgc
     2521 aattagaaat aaatctatct tttctcatac aataaatgca tgccgccaaa aatacactat
     2581 attttgaaga aaaacatcta agcatggaaa aaatcttcct cttcttgtca gaatgttttt
     2641 aatcataata tcctaaaaac acttatataa cagggataac gggtatataa tcctcgatat
     2701 aaggcaaaag tttacttgtg gtcgtggtat gtgataggga tagctcttta tatggaggat
     2761 atataaggga ttatatgggg gtggggcatc gaaacattta tatatgttta ataattgtca
     2821 atagtcaggg tcatggataa attaaaatgt atttagacat aaaaaaatcc gctaacctta
     2881 taaattatac attttaaaaa ggttaacgga cttttattgt tattattttg ttttaatact
     2941 taagttcttt ctgacaagga aaaagaattt tatttagata ttaaatatag aaatataaag
     3001 tggtttataa ttagttttgc ggttcatact gaacataaac tcctctatct aatagagtat
     3061 taatattatc tatatcatca gaacctacaa tgaggaccta tgattcatcg ctatataatc
     3121 accaactcca aagccatcat tttccatcaa cgggcttatg tctgagacct tattttcgga
     3181 gaatctcagc tcctccagat ttgtcagatt cgccagtggg cttaaaagta aaaacccatt
     3241 atttatagcc aggatctgta tttcttattt atcaaagaca accacacccc aatatgaaac
     3301 tttcaactta tggttcataa gttacttcaa atgattgaga ggcagtttga cctctatcat
     3361 ccattagagt aacggtataa tagagtgttc cagcccagga aggttcgttt tctattacta
     3421 taggttccag aatcattgaa gtatcagatt caattgtcac ggccaaattc cagtcattat
     3481 ctccctttat ccagtaacta cttccgctta ctgccccctt ccattcatac acaattgctt
     3541 gcacattttc gaaattactg ccactacagt aaagtttagc atggtaatca ggttcagtaa
     3601 cgtatatcgg atcattttct ggttctaaat ctgtgaaaag aagcccgata acttctattt
     3661 tatcttctaa aatgtcttga tctattatct ccgaatctac acttgccaat gatatggtat
     3721 tataccctat atcatcctca ataggctcgt ggttaaaact aatctctatt ctttctcccg
     3781 gatccaactc tatattctct tttgcttcgg cgtggtattt accaaaggaa aataatatat
     3841 cctgactacc cgcaccacca gtatttttaa cttctcctct gacagtaatc tcttcttcat
     3901 aatatacagg ttccaaagga taatcaataa tatccacttc aaagtaaggc ttttgattta
     3961 aaacttctag atcaacccag actttatcaa tagaatcata ttcattagtt gttttattaa
     4021 aaggcttaac aataacctct atctccgatc catcatcttc ctcagttata tagcttaatt
     4081 cgactatctc actttcacca ggcactaaac taacttcttt atcatcaaca aagctgccag
     4141 tactatcaat aaataaatca ataaatgctt caccaaattc atcaccatta tttgtaactt
     4201 ctacctctat attcatttca actccaatat ctactacaga agggtaatta ataattgtta
     4261 tattaaaaag tgggaatttt tcttcaactg gtacttttaa acttgtatta gcagtcttgc
     4321 catcgtcatc agttgcagtt attattacat cataagttcc ctcactatta taagtgtgat
     4381 tttcatctat actatcaaat gcattgctta tagtagttgt atttccatct ccccagtcta
     4441 tttctgctga tgaaacatta ccatcactgt ctgatatact gccacttatt gtcgctgtta
     4501 aattatttgt actactagat acatcgataa taggagtatt atttgtttca ctgacattaa
     4561 cagttaaact ctcactagca gttgctcctt tactatcggt tgcagaaacc ttgactgtat
     4621 aactcccact actagcataa atatggctct catttatact atcaaaccca ctacttatag
     4681 tagttgtatt ttcatctccc cagtctattg ttacttcctc tactgttcca tcactatcag
     4741 ttatcggtcc acttatagtt gctgttaaat tatcagtgct tgatgatatg tcaataatag
     4801 gaggattatt ctcttcttca aatacaacag taatattttt atcctcattc attgttatcg
     4861 ttgtggtagg attagtactg gatatatcac cagtccattc cttaaacttc cagtcatcag
     4921 acgtaagagt ttctaaattg actatatcac cttctttaaa tccattttta taaggaaggc
     4981 taaattccat atcattaact agtattatac cttcaccttc tttttctatg gttaaggtag
     5041 ggccatctat agtagtaaac tcccagacat cactttcact gatgccgccc tttccatctt
     5101 tagcaactac tttccaataa taggtagtat tttggcttaa acccgatact tcataactag
     5161 tagaacttat actatctgct acttttgtaa aaagagcact agaagttcct aaataaacat
     5221 cataggtaac actataaact tctggagctg tagaaacccc ccaggacaat atcaagtttg
     5281 tatctacatc agcgactcca ttagaaggag attttagaat acatgtgaga ggatttaccg
     5341 cccctataga accttcccag ggggttattc ctgcagctgt tcctatcctt gtacctaatt
     5401 cccccgaagt agcaatcata cctggaatat ctaccgcttt aacaaattgt tttccagcaa
     5461 atttgagtat tgatttcaat ccctttttta ctattttatt ggctacctgc ttagatattc
     5521 tttttgacaa ttgcataaca gctttattaa atacatggtt tgttgataag actgtcttga
     5581 tggaaactcc tgcttcaaaa ttttgtacaa atgattcgca aatctcttgt agctcttcag
     5641 tataatattg aggctctaca taatccataa cagcaaaaga aagagaaact gacttcaatg
     5701 atatttctaa agcatttact gaacaagcag ttgctaatcc ccattttgaa taaggccaat
     5761 ttcttattaa ttccatatcc tttttcttgt tctcataatt atgtaatcct gctcccatag
     5821 tataaaggtg taaggcataa cctccttctt cttctgaaaa tctatattta ttctctccgg
     5881 gaatattatc attatcaaaa atattatcat tctcaaaaat tttatcaaca atataatcaa
     5941 taccaacaga aagtaaatca atatatccaa aaacagaatc cgcgcctaga tatattttgc
     6001 caacaggacg attactctct acctgtatat ctgctctgtg aggattactc tcccagtcaa
     6061 ctttaaaatc gttccaggaa gaatattggg atttatcaat tttatatata aatccaaccc
     6121 aggaaatttc tcttccggaa aatgtatctg ccttggcctg taaaacataa ttatcta
//
LOCUS       DATGCJ010000238        25060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_37868, whole genome shotgun sequence.
ACCESSION   DATGCJ010000238 DATGCJ010000000
VERSION     DATGCJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 25060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25060
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37868"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..501
                     /gene="msrB"
                     /locus_tag="VKN64_10525"
     CDS             88..501
                     /gene="msrB"
                     /locus_tag="VKN64_10525"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMA60720.1"
                     /translation="MSENKEKFPVNKTEKEWKEILTKREFLVLRKGKTDPPYKNEYYH
                     FDENGIFKCAACDNELFDSEDKYDSGSGWPSFIRPIREAAVDYRDDYKLSHKRTEVIC
                     SQCGSHLGHRFEDGPEPTGFRFCINSTSLNFEERK"
     gene            600..1535
                     /locus_tag="VKN64_10530"
     CDS             600..1535
                     /locus_tag="VKN64_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA60721.1"
                     /translation="MEYLYGPVPSRRLGLSLGIDLIPYKICSFNCIYCECGETTNLTS
                     TRKEFKPVDKIIAELDEYLQDDPELDYITFSGSGEPTLYLHIGKIIDFLKENYPKYDT
                     ALLTNSSLLSHKEIREELKNLDLIVPSLDSVIEENYQKINRPHQDIKLTDIIDGLIKL
                     REEFSGKIFLEVFIVPGINDNEEEYHKFKEVIEKIKPDRVQLNTLDRPGTEDWVEPAL
                     EKDLNKAVNIIGKKAEIIGKYSPTKKKKGMSHAVKKRIVETIRVRPCTVKDLSEILDL
                     HVNEVNKYLREIDKEYNLKMKEQTRGRFYFLSKEE"
     gene            1538..1762
                     /locus_tag="VKN64_10535"
     CDS             1538..1762
                     /locus_tag="VKN64_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HMA60722.1"
                     /translation="MANQIKVDCVGENCPVPLVETRKAIRKAEEGDIITVTGDHSASK
                     KEIPMAVDSMGLELLSVEDKNDHWEIKIKV"
     gene            1774..2193
                     /locus_tag="VKN64_10540"
     CDS             1774..2193
                     /locus_tag="VKN64_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HMA60723.1"
                     /translation="MEDKATLIIESGDYDKLYSAFIIGNGALSMGMDVSMYFTFWGLE
                     RLKKGQLDKGPLSKMNFLGLGKLYMKWKMKKANVRPLEDMLQDYVELGGKIFACDMTI
                     DIMGINKEELRDEVITEYCAVGTYIKEARDSEITLYI"
     gene            2210..3379
                     /locus_tag="VKN64_10545"
     CDS             2210..3379
                     /locus_tag="VKN64_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMA60724.1"
                     /translation="MNKDMVYLDNSAVTRIDEKVLEAMKPYFFDTYAVATSEFAYTMG
                     IEAKEALEEARATIADSLQADPEELIFTSGATESNNLALKGVMYNQKEKGHLIVADIE
                     DFSVLNSAKKLEKQGYEVSYISVDGKGKVNIEELKDSIREDTVLVSIQHANQEIGTVQ
                     NIKEIADYLKEKDILFHTDATHTFKRIPIDTREIPVDLVTISAHTIHGPRGIGALYKR
                     KGVKLTKIMDGGFQENDLRAGLENIPSAVGFAKAVELVTEEENEKIKSLRDRLINKVE
                     EEIEDVTLNGAREDRVPQNANITFHYVEGESITLHLDMRGFAVSTGSACFSKSLEASH
                     VILGIGGDHERAHGSIRFTFSRYNTEEDVDKIVEAVKDVVENLREISPLGKKGSE"
     gene            3382..4017
                     /locus_tag="VKN64_10550"
     CDS             3382..4017
                     /locus_tag="VKN64_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HMA60725.1"
                     /translation="MSSPYSDKVIEYFQNPKNVGELEDPDGIAKEGSPACGDMVQLHL
                     KVDEDTQKITDIKFRSYGCASNIATGSIITEMAKGSTIEKAKDITWQQAADELGGLPA
                     VKNHCSVLAVDALKEAIRDYEEKHGLVKERVPTTLEVVKERLKHVMNPIHGLDVLKTN
                     LIEDIEIDDKTITVQVDLAEDHQFAPVVKEDIKEKLEPRWDIEEVIVKYKE"
     gene            4129..4914
                     /locus_tag="VKN64_10555"
     CDS             4129..4914
                     /locus_tag="VKN64_10555"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMA60726.1"
                     /translation="MTVINLEKTKEDVKSLAREVGSYQLKKLDQKLNVSQKSTYLDLV
                     SEVDKKAEEKIVNWIESNFPEHSILAEESGTSDKESKYKWVIDPLDGTTNYVHGFPIF
                     SISIALMENETPVLGVIYIPYLKEMYTAVNNKGAYLNGKPLKISKKSDLKESLLVTGF
                     PYNLTEDEYNNINIFSTLLLKARGIRRIGSAAYDLACVAAGKLDAFWELKLSPWDVKA
                     GIVIIKEAGGKVIETDYHGHYLIVAGPAELTDKLYEEIRKVHN"
     gene            complement(4993..6774)
                     /locus_tag="VKN64_10560"
     CDS             complement(4993..6774)
                     /locus_tag="VKN64_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMA60727.1"
                     /translation="MSKRIVILGAGYAGIEAAKNLNKKFREDPDVQIDLINDRDKHIL
                     LTELHEVAGNRVDKSGVEISLDQLFENTKVNVIKDKIIDFDLANNKIASSDQEYEYDH
                     LILGVGSEPTFYNIPGMEHNSYTLWSEDDAVELREHVRNMFKLAANESDLEKRKKMLT
                     FVIGGGGFTGIETAGELAEWFDDLCYTYDISRSEVNLIVVEALERILPVLCDEQCDFA
                     EDYLSEELNVEIIDGYAICNVTKDYIEISKTGKDTDVRKRIDSNTVIWTGGVKGKEIL
                     KKTDLELSRDNRIKVDKYLQTTKYDNVYAIGDNALFTHDRERPLPQLVEAAVQGGECV
                     AENIEAKMKNKSLEVFEPNMHGLMVSVGANYGVAELKPFGDFTIALKGFWAMFMKHFI
                     NMHYLFGIGGFSLIGDYIEHQFKDTRGGIGMLVRHMMKKSGTYWLAFLRIFVGLRFLL
                     SGIDKINKGWLFGSEEFLVSGASSYLWSAGTPQFYIDLMETFVVPNQVFFQKILVVTE
                     LVTGLALIAGFLTFLFALVSIGMSINFVIGAIGSSAGIWEPLWILLISITLLAGAGKA
                     FGLDYYLIPWLKNISKKPVSYRKERSA"
     gene            7010..7714
                     /locus_tag="VKN64_10565"
     CDS             7010..7714
                     /locus_tag="VKN64_10565"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HMA60728.1"
                     /translation="MRKITKKSILSLIAVFVLVAAFSGGAAAQDFNDGEYVGYVPGDH
                     GDIVIEVKVNFGEITGVEILNNLKTTQYPHEPAREYFINFPSAVIRNQGTEGIDAVSG
                     ATGSHNSYTEAVDMALSIARGNYDGNTYYGVSRDYAHGHYLVEAVIEGEEIKDVSIIT
                     AKNPEEEALVGPKKPGEYDYEPAVETFQKWADMVVENQSVNVDVVSGATHTDHGLNGA
                     LAQCLTQAGLNPDDYK"
     gene            7811..8902
                     /locus_tag="VKN64_10570"
     CDS             7811..8902
                     /locus_tag="VKN64_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HMA60729.1"
                     /translation="MNYKRFLVAIIILIIIIGGMYFIGNNNPGNRNNTIEPNGDVNNK
                     VEVTKTDGSEFLMDTLVRLRIHDEQPQQILDETFNKLRYLENIFSKTITESDVSKINS
                     NAGQKAVEVSDDTYKLLEESYQFAEKTNGVFDPTIGPLVSLWGIGTEDQQVPTEEEIQ
                     NTLKKVDYRKLELRDDNKVFLKEEGMSIDLGAIAKGFAADYVLNYFNEKDVDSAFINM
                     GGNVSVHRDKTDDTLWSIGIQDPDQTRGSIIAAVEGEDMSVVTSGNYERYFTKDGIRY
                     HHILNPETGYPAREGIISSTVISNNSSYADALSTSFYILGIEKSFEIAKSMEDINIVL
                     VTEDNKAYVSKELKDRIRFTTEELEVIYR"
     gene            8931..9911
                     /locus_tag="VKN64_10575"
     CDS             8931..9911
                     /locus_tag="VKN64_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMA60730.1"
                     /translation="MFWDQYPDFAEELNSFEKYFRNQLSSSNELITEEIKRLAEAGGK
                     RVRPALLISVAHLGEYKQDRIWPMAAAIEMIHLATLIHDDVIDKSSLRRGVKTTHQKY
                     TNKTAIFMGDYLFALSMQIILEEFGNEKIDGALLRQMKNTIKSIKSLCEGEVSQFQRQ
                     FEKIPTFKEYLSAIKRKTALLFKASAMLGADLSGMKKYQVFKMGKLGFNMGMAFQMVD
                     DLFDFQEKNQQLGKPQKNDFTQGIYTLPIIYIYNEVENKEKLEKFLKEPEKHSEEIKK
                     LVHEKGGINYTKKLAFRYKEKAQKIIKPYTDNKYGEIINWLTEKIIGRNF"
     gene            10002..12269
                     /locus_tag="VKN64_10580"
     CDS             10002..12269
                     /locus_tag="VKN64_10580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMA60731.1"
                     /translation="MNIEQMKSHLQNKPKFMNRITAWREIEGRDGKYSDLPVNLDQRL
                     KEVLKTQDIDRLYSHQKEAYNYINNEKDTVVVTPTASGKTLCYNLPVLNSILEDDNNR
                     ALYMFPTKALSQDQLSGLYDLIRALDEDIKTYTYDGDTQVSARKSIRESGHIVITNPD
                     MLHVGILPHHTKWIKLFENLKYVVIDEVHSYRGVFGSHVANVIRRLKRICNFYGSDPI
                     FICASATIDNPGELTSKLIGKEVEVVDENGAPAGPRDFIFYNPPVVNKELGIRRSYVL
                     ESKKIASIFLKNKIKTIIFTRSRLRTEIILSYLRDDKPRDVNIRGYRGGYLPSERRQI
                     EKGLREGDIHGVVSTNALELGVDIGQLDVSILAGYPGTIASSWQQAGRAGRGQRRSIS
                     VLVASSSALDQYIVNNPEYFFEQPAESALINPNNLMILLSHIKCAAFELPFEEGEQFG
                     VETTDEILNYLEDNNVLRYKGGKWYWAAERYPAEDISLRSASSDDFAVIDRTKTGSPR
                     VIGRVDYFSVPTTIHEDAIYIHEGKQYQVKELDYDGKKAYVKEVDVNFYTDANLAVDL
                     SVLDQFDKKELSKRTAEHGEVVVSAQATMYKKVKFNTHENVGYGDINLPEVEMHTTAW
                     WMSLDKEFEEKLNKKVLENALLGLSNLLENIAPIYLMCDPGDINSTVQIRSPFTSKPT
                     IYIYDSYPGGVGLSEKLYRIDYKLLKSAYKYLQKCPCEAGCPACVGPINEVGLNGKEK
                     TLYLLKEVLQGESEG"
     gene            12253..13473
                     /locus_tag="VKN64_10585"
     CDS             12253..13473
                     /locus_tag="VKN64_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMA60732.1"
                     /translation="MNLRDKLKKFEKNQKPHRSKEEIEYKNKDAFNNQLISNNMGTYL
                     KSVNDYKNEYKHGLYQIDQLKEQLDTEILFKIANNKSTKLNDEIIGNLLFIDTETTGL
                     MGGTGTVSFLIGAGYFTAGDFVIEQYLMRDYDEEAAMLQDFKEIMGNHNIIVSFNGKS
                     FDLPLIKTRLIMNRFDRPNYDYHLDLLHSARRLWSFLDSCSLKSLERNILNFERIDDV
                     PGHLIPGLYFEFLENKNLELLAPVLEHNIYDILSLVTLFTHLKEIHLDNVDNLDSNEL
                     FHLGRIKEKEKNYHDCIDYLEASVENSEESTLKYRALKKLSWQYKRIDRYDKAAEIWK
                     EMIDQKKQGIFPYVEMAKYLEHKKKNFKEALNYTNQAIEMLNERRIIINNFDKKIKNL
                     EHRKERLIKKSSKI"
     gene            13537..14406
                     /locus_tag="VKN64_10590"
     CDS             13537..14406
                     /locus_tag="VKN64_10590"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HMA60733.1"
                     /translation="MEEKRVNTFSIVGYDPDKEELGIAVQSKFLAVGAVVPWAKAKVG
                     AIATQALANLDYGEEGLKLLEEGYSPAEVIKKLRAEDENDQSRQVGVVSATGKSASFT
                     GDDCYDWAGGITGDNFACQGNILVGEDTVQAMADTFTNTEGDLADRLVTALEAGQKAG
                     GDKRGRQSAALLVVKENGSYGGYNDRYIDLRVDDHETPIQELKRLLDIFYLYFGDKEV
                     EKVELTDEVIKEVKQYLKNLDLFSGKIDAENNEEFKKSLESFYHQENFEERKQKEGFL
                     DKDILNYMKDKAE"
     gene            14427..14576
                     /locus_tag="VKN64_10595"
     CDS             14427..14576
                     /locus_tag="VKN64_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60734.1"
                     /translation="MDRKEFIIEIDGMSDRKLRQLLNFVYEEFEKEDKVDLLKDKIDE
                     IKKFS"
     gene            14771..14856
                     /locus_tag="VKN64_10600"
     tRNA            14771..14856
                     /locus_tag="VKN64_10600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14805..14807,aa:Leu,seq:tag)
     gene            14977..16257
                     /gene="tig"
                     /locus_tag="VKN64_10605"
     CDS             14977..16257
                     /gene="tig"
                     /locus_tag="VKN64_10605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMA60735.1"
                     /translation="MEVTKNQLEGNKVELKVEIEAERVNEALEQAYKKVVKDIDIDGF
                     RKGKVPRRVLEARYGKEILHKDALDILIPEGYREALEETGIEPIDQPDIEDYHIAEDE
                     PASFTAVVEVKPEVELGEYKELGIEKESAEVSEEEIQEEIDKVRNQHSQLVASDKEVV
                     EDGDFVIIDFEGKKDGEPFPGGSAEEYSLEIGSNTFIPGFEEQLIGAKVGDELELNIT
                     FPEDYNAKDLAGEEVVFDVEIKELKEKELPELDDEFAKEVSDYETFEEYKESIKERLQ
                     ENKEERTEREYENKLIEKASENAIVDVPEKMVEDELNNMFQNFAQSVSQQGMEVEDYL
                     DYMGTDEEGWKEQNKEAAENRTRSNLVLEAIAEQEDIEVSEEEIEEQIQEIAESNDQD
                     PEQIKAFLQMQGQLDGLKDGLQMQKTIEYLKENN"
     gene            16286..16894
                     /gene="clpP"
                     /locus_tag="VKN64_10610"
     CDS             16286..16894
                     /gene="clpP"
                     /locus_tag="VKN64_10610"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HMA60736.1"
                     /translation="MSGLIPMVVEQTNRGERSYDIYSRLLKDRIVFLGSHVTDDIANL
                     IIAQLLFLEADDPDKDIYLYINSPGGSVTAALAIYDTMQYIKPDVVTICMGQAASAGA
                     LLLAAGEDGKRYGLPYARVMIHQPAGGVQGKATEAEVHIKELMRIKDLLNDILSDHTG
                     QPVEKISEDVEKDYFMTSEQAVEYGIIDKVISRSEMKDNKEE"
     gene            16911..18176
                     /gene="clpX"
                     /locus_tag="VKN64_10615"
     CDS             16911..18176
                     /gene="clpX"
                     /locus_tag="VKN64_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMA60737.1"
                     /translation="MFKFDDEDGQLECSFCGKSQDEVKKLIAGPGVYICNECVDLCND
                     ILKEELSVDLDLNFSNIPKPREIKDILDQYVIGQDRAKKTLSVAVYNHYKRINSDMKI
                     DEVELQKSNILMVGPTGCGKTLLAQTLARILDVPFAITDATSLTEAGYVGEDVENILL
                     KLIQAADYDIEKAEKGIIYVDEIDKLARKSENPSITRDVSGEGVQQALLKIIEGTTAN
                     VPPKGGRKHPHQQFIQIDTTNILFVFGGAFDGLEKVIKSRISEKVMGFGADIKTKDEK
                     ENIGDILKHVLPQDLLHYGLIPEFIGRMPVIATLDELSVDDLVQILTEPKNALTKQYK
                     KFFEMDHVDLEFTEGALRAIARKTLEVNTGARGLRSVVEDTILEIMYDLPSQDDITKC
                     IIDEGVIEKSKEPKLIRKSDVGSDREESA"
     gene            18342..20714
                     /gene="lon"
                     /locus_tag="VKN64_10620"
     CDS             18342..20714
                     /gene="lon"
                     /locus_tag="VKN64_10620"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMA60738.1"
                     /translation="MKKDLEEVKISDGDIEKIDDDFIELPLLASRGVVVFPHMVIPLL
                     VGRDKSVEALEKAMLEEKRILIAAQKDESIEEPVVEDLYNVGTITEVKQLVKLPNGMI
                     KVIVEGIQRAQIKEYLKTDEYFKVKAEVFNEEAEQYIEEDYEIEALMRSVVDYFKEYV
                     KYNRNFPAETMVSVINIEEPGRLADIIASNLELKYKKEQELLEAFSHKERLELLMKII
                     KKEIEILKIENDINEKVKDQVEKTQKEYFLKEKLKVIKEELDEEDMDEIEEYRAQMQE
                     IDFPEEVEEKMEKEIKKLNKTPSMSPESTVIRNYLDCMIDLPWDEEKNTEINIKEAEK
                     ILEEDHYGLEDVKERIIEYLAVRKISKKKKSPILCLVGAPGVGKTSLGRSVARALERD
                     FVRMSLGGVRDEAEIRGHRRTYIGSRPGRIINSMRDAGSKNPIFLLDEVDKMSADFRG
                     DPASALLEVLDPEQNSEFTDHYLEVPFDLSDVLFITTANVTSTIPAPLLDRMEVIELP
                     GYTEDEKVKIAERHLIPRILKEHGLEKAQINISDNALYKIIRDYTREAGVRNLEREIS
                     AIVRKISKEIVEGRDKKARITKRNVDKYLGVRKFKYEVAKKEDRVGVATGLAYNQAGG
                     DILDIEVAVVPGKGEVTLTGSLGDVMQESAKAALSYIRSKYKELNLEKDFHENYDLHI
                     HVPQGAVPKDGPSAGITIALAIASALTNRKVKGDYAMTGEISLRGRVLPVGGIKTKIL
                     AAKRAGINKIVLPKENEESMEEIQTYLKKDLEINYVEHIDEVIDLLLLEE"
     gene            20718..21302
                     /gene="yihA"
                     /locus_tag="VKN64_10625"
     CDS             20718..21302
                     /gene="yihA"
                     /locus_tag="VKN64_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMA60739.1"
                     /translation="MKINNPEFVKSAHTIEDYPHNNLPEIAFAGRSNVGKSSMINKVL
                     NRKGLARISSKPGHTRSINFYNIDNNFYLVDLPGYGFAKVSKEEKDKWSMLIDDYLHY
                     RENLAGLVMIVDSRHKPTEQDKVMLNWIREVNIPKIITATKADKISNNKKAKQKKVIK
                     DVLDLRADERFTFFSAKTGEGKKDIFSFINHIIK"
     gene            21313..22137
                     /locus_tag="VKN64_10630"
     CDS             21313..22137
                     /locus_tag="VKN64_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMA60740.1"
                     /translation="MESQSYTSSFAVIYDDVMQRVPYYYWYKYLKHLLAYYDKKPEKI
                     MELACGTGNMIKYFAKEADSIYGIDKSKDMLTVAQNKFSENDNVELFNTDMSEKYKYG
                     EFDLIYSIFDSLNYILEYEDLLRVFANAHYNLNQDGIFIFDMNTIYRLMDISEGTNKI
                     EGDDYTCYWRDIVDREKKEWIVELNIYLDRGENIENFTEKHVETSYSLKKIKEALLEA
                     GFKHVDYYKSFTFRSGKPQNNRIHFAAMKKKPESKSIKNFLIKLKWNLISPFISSF"
     gene            22191..22267
                     /locus_tag="VKN64_10635"
     tRNA            22191..22267
                     /locus_tag="VKN64_10635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22225..22227,aa:Arg,seq:cct)
     gene            22732..23208
                     /locus_tag="VKN64_10640"
     CDS             22732..23208
                     /locus_tag="VKN64_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60741.1"
                     /translation="MKYVYLGKEEVNGTETDKISIEIADKIQEDTPSKLYFWFADDNI
                     KQMQIDEQVLSENMAQMMADRFLSAIFSPLKQLSQYDIAEFRKIGKVTKYKEKYSGRE
                     LNITKIEVEDIPEYELEYGMTEIAELEETAVVLEYQYISSENKNKVEFKVKDFQLH"
     gene            23465..24760
                     /locus_tag="VKN64_10645"
     CDS             23465..24760
                     /locus_tag="VKN64_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HMA60742.1"
                     /translation="MPKIAFMGAGSTIFAKNVIGDCLLTPALRESEFALYDIDHKRLK
                     ESAMMLKNINHNYGNHGTIKAYKDRKKALKNADYIINAIQVGGYKPSTVIDFEIPKKY
                     GLRQTIGDTIGIGGIFRALRTIPVMLDFARDIQDVAPEALFLNYTNPMAILTGAMLKA
                     TEVNTVGLCHSVQDCLNSLLEPLGMSTENVRHKIAGINHMAWLLEVSRDGKDLYPEIK
                     KKAFNREKTHDDRVRYEIMKRFGYYVTESSEHNAEYLPYFIKSEYPELIEKYNIPLDE
                     YPRRCEEQIAEWEEMRDSLVDNADLEHELSNEYASYIMEAIETDQPYRIGGNVVNTKL
                     IENLPEEAIVEVPCLVDRNGITPCHVGRLPEQLAALNRTNINVHIMTIKAALEKKREY
                     IYQAALLDPHTSSELSIEDTINLCDDLIEAHGDWLPKYS"
     gene            complement(24803..>25060)
                     /locus_tag="VKN64_10650"
     CDS             complement(24803..>25060)
                     /locus_tag="VKN64_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60743.1"
                     /translation="LYFIKTTDDFKYLDKVKQVYKKEKYYYKITGYKPLQEFSIRRND
                     DIIETIRTKDTLHYFQLKTNNLLDSDSYLWEYFILKGYKPE"
BASE COUNT         9215 a         3420 c         4979 g         7446 t
ORIGIN      
        1 cttatccaac tcaaagtgta catgtttatg ataatgcgga tgacagtgat attcagcccg
       61 ttgagaggat aaaggaggaa gaaaataatg agtgaaaata aagaaaagtt tcctgttaat
      121 aaaaccgaaa aagaatggaa agagatttta accaagcgag aatttttggt attaagaaag
      181 ggtaaaacag atccccccta taaaaatgag tattatcatt ttgatgagaa tggtatattt
      241 aaatgtgcag cctgtgataa cgagcttttt gattctgaag ataaatatga ttcgggttcc
      301 ggttggccca gttttatcag gccgattaga gaggctgcgg tcgattaccg agatgactat
      361 aaactgtctc acaaaagaac cgaagtaatc tgcagtcaat gtggaagtca tttgggacat
      421 cgttttgaag acggccctga accgaccggt tttaggtttt gtattaattc aacatcactt
      481 aattttgaag aacgtaaata gtttgtaaaa tataaacttg actaaaatac tcggatatca
      541 tataatagca tcagcataga tgctgttcct taaaaactaa ataaaaggaa ggtgtaaaaa
      601 tggaatattt atatggacct gttccctcca gaagactggg attatcactc ggtattgatc
      661 ttatcccata taagatctgt tcttttaact gtatttattg tgaatgtgga gaaactacta
      721 atcttacaag tactagaaaa gaattcaaac ctgtagataa aattattgca gaactagatg
      781 aatacctaca agatgatccc gaattagatt atattacttt ttctggttca ggggagccaa
      841 ccctatattt acatatagga aaaattattg attttttaaa agaaaactat ccgaaatacg
      901 ataccgccct tttaacaaac agcagcctat tatcgcataa agaaattaga gaagaattaa
      961 aaaatcttga tttaattgtc ccttctttag attctgtgat agaagaaaat tatcaaaaga
     1021 tcaacagacc tcatcaagat attaaactta cagatatcat agatggttta attaagttaa
     1081 gagaagaatt ttcaggtaaa atatttttgg aagtttttat tgtacccggc ataaatgata
     1141 atgaagaaga gtaccataaa tttaaagagg ttattgaaaa gattaagccc gacagggttc
     1201 aacttaatac ccttgataga ccgggtacgg aagattgggt agaaccagct ttagaaaaag
     1261 atttaaataa agcggttaat ataataggta agaaggctga aataattggt aagtattcac
     1321 ccactaaaaa gaaaaaaggc atgagtcatg ctgttaaaaa aagaatagtg gaaactatca
     1381 gagtaaggcc atgtactgta aaagatctat cagaaatttt agacctacat gtgaatgaag
     1441 taaataaata cttaagagaa attgataagg aatataattt aaagatgaaa gaacagacca
     1501 ggggaaggtt ttactttctg agtaaggagg aataatcatg gctaatcaaa taaaagtaga
     1561 ctgtgtagga gaaaactgtc cagtacctct agtagaaacc cgcaaagcaa tccgtaaagc
     1621 ggaggaaggt gacataataa cggtcaccgg tgatcactct gcttctaaaa aggagattcc
     1681 aatggctgtt gattctatgg gtttagaatt gctgtctgtt gaagataaaa atgaccattg
     1741 ggaaataaaa attaaggtat agaggtgtaa aatatggaag ataaggccac tttaattata
     1801 gaaagtggag attatgataa gttatacagt gcctttatta taggtaatgg agcattatca
     1861 atgggcatgg atgtttctat gtattttaca ttttggggcc ttgaaaggct gaaaaaaggg
     1921 caacttgata aaggtcctct ttctaagatg aattttttgg gccttggtaa attatatatg
     1981 aaatggaaga tgaaaaaagc gaatgtccgc ccactggaag atatgttaca ggattatgtg
     2041 gaattaggtg gtaagatatt tgcctgtgat atgactatag atataatggg aattaataaa
     2101 gaagaattaa gggatgaagt tattactgaa tactgtgctg tgggaactta tattaaagaa
     2161 gccagggatt cagaaataac tttatatata taaaaattga ggtgatttga tgaataagga
     2221 tatggtttat cttgacaact cggctgttac tagaattgat gaaaaagtat tagaagctat
     2281 gaaaccttac ttttttgaca cttatgcggt ggcaacttct gagttcgcct atacaatggg
     2341 tattgaggcc aaagaagctt tagaagaggc aagagcaaca atagctgatt ccttacaggc
     2401 cgatcctgag gagttaatat ttacatcggg tgctacggaa tccaataact tagcattaaa
     2461 aggcgtaatg tacaaccaaa aagaaaaagg acacttgata gtggctgata ttgaagattt
     2521 ttcagttcta aattcagcca aaaaattaga aaaacagggt tatgaagtta gttatattag
     2581 tgtggatggg aaaggtaaag ttaatataga agaattaaag gatagcatta gagaagatac
     2641 tgtattagta tctattcaac atgccaacca ggaaattggt actgtccaaa atattaaaga
     2701 aatagccgat tatcttaaag aaaaggatat cctatttcat actgatgcaa ctcatacctt
     2761 taaaaggatc cctatcgaca ccagagaaat accagttgat ctcgtaacca tttctgctca
     2821 tactatccat ggaccgaggg gtattggggc actttataaa agaaaaggtg ttaaattaac
     2881 caaaattatg gatggtggat ttcaggaaaa tgatttaaga gcgggactgg aaaatattcc
     2941 ctcagctgta ggttttgcta aagctgttga attggtaaca gaagaagaaa acgaaaagat
     3001 taaatcttta agagatagat tgataaataa agtagaagaa gagattgaag atgtgacatt
     3061 aaatggtgcg agagaagaca gagttcctca gaatgcaaat atcacttttc attatgtaga
     3121 aggtgaatca attaccctcc acttagacat gaggggattt gcagtaagta caggttctgc
     3181 ttgttttagt aaatcactgg aagctagcca tgtaatactt ggtataggtg gggaccatga
     3241 aagggcccac ggttcaatca gatttacttt cagcagatat aatactgagg aagacgtaga
     3301 taaaattgtg gaagcggtaa aagatgttgt tgaaaattta agagaaatta gtccattagg
     3361 taagaaagga agtgaataat aatgagttct ccctatagtg ataaggttat agaatatttt
     3421 caaaatccca aaaatgtagg tgaattagaa gatcccgatg gtattgcaaa agaaggttcg
     3481 cctgcctgtg gtgatatggt acaacttcat cttaaagttg atgaagatac tcagaaaatt
     3541 acggatatta aattccgatc atatggatgt gcttctaata tagcaacagg ttctattatt
     3601 acagagatgg ccaagggtag tacgattgaa aaagcaaaag atattacctg gcagcaggct
     3661 gctgatgaat taggtggttt gcctgcggtt aaaaaccact gttctgtatt ggccgttgat
     3721 gccttaaaag aagctatacg agattatgaa gaaaaacatg gtttggttaa agaaagagtt
     3781 cctactacct tagaagtagt aaaagaaaga ctaaaacacg tgatgaatcc cattcatgga
     3841 cttgatgttt taaaaactaa tttaatagaa gatatagaga tagatgataa aacaattaca
     3901 gtacaagtag atctggctga ggatcatcag ttcgcgccag ttgtaaaaga ggacatcaaa
     3961 gaaaaattgg agccgcgttg ggatatcgaa gaagttatag ttaaatataa agaataaagc
     4021 aagaattgcc cgggtagccg ggcaattctt ttatcaaaaa gtttaattaa tatttaaaaa
     4081 atccttatta tatattaaga tgatagatag aaagacatat aattggaggt gactgttatt
     4141 aatctggaaa aaactaaaga agatgttaaa tcattagcta gagaagtagg tagctatcag
     4201 ttaaagaaat tagatcaaaa attaaatgta tcgcaaaaat caacctattt ggatttagtt
     4261 tcagaagtag ataaaaaggc ggaagaaaaa atagtaaact ggatagaaag taattttcct
     4321 gaacatagta ttttagcaga agaaagcggg acttctgata aagagtctaa atataaatgg
     4381 gtaattgatc ctttagatgg aactaccaac tatgttcatg gttttcccat tttttcaatt
     4441 tccatagctt taatggaaaa tgaaactccc gttttaggag ttatttatat accttattta
     4501 aaagaaatgt atacagctgt aaataataag ggggcatatt taaatggtaa acctcttaaa
     4561 ataagtaaaa aaagtgatct taaagaaagt cttttagtta ccgggtttcc ttacaatctg
     4621 accgaagatg aatataataa tattaatatc tttagtactc tcttgttaaa agcaagaggt
     4681 attagaagaa ttggtagtgc cgcctatgat ttagcctgtg tagcagctgg taagttagat
     4741 gcattttggg aattaaaact aagcccctgg gatgtgaagg ccggcatagt aataataaaa
     4801 gaagcaggtg ggaaggttat agaaactgat tatcacggac attatttaat agtagctggc
     4861 ccggcagaac taacagataa gctttatgaa gagattagaa aagttcataa ttaaataata
     4921 gtctaaataa agaaaaaaca aaaaaggaga gcttttaaaa gctctccttt gatatttaat
     4981 atttatttta ttttaagcag atctttcttt tcgatagctt accggtttct ttgagatatt
     5041 tttaagccac ggtattagat agtaatcaag accaaaggct ttacctgcac cggcaagtag
     5101 tgttatggaa atcaagagta tccagagcgg ttcccagata ccggctgatg agccaattgc
     5161 tccgataaca aagttgatac tcattccgat tgaaactaag gcgaatagga atgttaagaa
     5221 accagctatt aaagccagtc cggtaactag ttcagtaacc accagtattt tttggaaaaa
     5281 gacctgattg ggtacaacaa atgtttccat taagtcaata tagaactgtg gtgtaccagc
     5341 actccagaga taagatgaag cgcctgatac taggaactct tctgaaccaa ataaccaacc
     5401 tttgttaatc ttatctatac ctgacaataa gaaccttaga ccaacaaata ttcttaaaaa
     5461 tgccagccaa taggttccgg attttttcat catatgtctt accagcatac caatacctcc
     5521 tcgtgtatct ttaaattgat gttctatata atcgcctatt aaactgaatc cgccgattcc
     5581 aaataaataa tgcatgttaa taaagtgttt cataaacatt gcccaaaaac ctttaagggc
     5641 aattgtaaaa tcaccaaatg gtttgagttc agccacacca tagttggccc caactgaaac
     5701 cataagtccg tgcatattag gttcaaaaac ttctaatgac ttgtttttca tttttgcttc
     5761 aatattttct gcaacacatt ccccaccctg aactgcggcc tcaaccaatt gaggtaaggg
     5821 gcgttcgcgg tcatgtgtaa acagagcatt atctccaatt gcataaacat tatcatattt
     5881 ggtggtttgt aggtacttat cgactttaat ccggttatct ctacttaatt caagatcggt
     5941 ttttttcagg atctctttac ctttaactcc acctgtccag attactgtat tggaatcaat
     6001 tcttttcctt acatcagtat ctttgcctgt tttgcttatt tcaatataat cttttgttac
     6061 attacaaatt gcgtaaccat caattatttc cacatttagt tcctcactaa gataatcttc
     6121 tgcgaagtca cactgctcat cacagaggac aggtaaaatt ctttctaagg cctcgactac
     6181 tattaaatta acttcgcttc tagagatatc ataggtataa catagatcat caaaccattc
     6241 agctaattca ccagctgttt caataccggt aaatccgcca ccaccgatca caaaggtaag
     6301 catctttttc cgtttttcaa ggtctgattc attggctgct agtttgaaca tatttcttac
     6361 atgttctctt aattctacag catcatcttc tgaccaaagt gtatatgaat tgtgttccat
     6421 acctggtata ttataaaaag taggttcact acctacaccc aggattaaat ggtcatattc
     6481 atattcctgg tcgctagaag caattttatt atttgccagg tcgaagtcaa taattttatc
     6541 ttttattaca ttaactttag tattttcaaa gagctggtct aaagaaattt caactccgct
     6601 tttatcgacc ctgtttccag caacttcgtg taactcggtt agtaagatat gtttatcacg
     6661 gtcattgata agatcgattt ggacatctgg atcttcacga aattttttgt tcagattttt
     6721 tgctgcttct ataccggcat atcctgcccc gagtattact attcgcttgc tcatataatc
     6781 cccctttact taatattatg atttgttttg tgaattgaaa tacatattta aatatataat
     6841 tcacaataga ttttattggt ttatatcttt tctcaattta aataataact tatttactaa
     6901 ggattttcaa gaaaaacctg agttttttta taataattct agatataatt tgcattttaa
     6961 gaaattatta attataatat attacagaat taaaaaagga gggatggctg tgagaaaaat
     7021 cacaaaaaaa tcaatcttat ctttaattgc agtatttgta ttagtagcag ctttttcagg
     7081 gggggcagca gcacaagatt tcaatgatgg agaatatgta ggatatgttc caggcgacca
     7141 tggtgatata gtaattgaag tcaaagttaa ttttggcgaa attactggag tagaaatttt
     7201 aaataattta aaaactactc agtatccaca tgaacctgcc cgtgaatact ttatcaactt
     7261 tccttctgcg gtaatcagaa accaaggaac agaaggtatt gacgcagttt caggtgctac
     7321 tggctcacat aattcttata cagaagctgt agatatggct ctaagtattg cgagaggaaa
     7381 ctatgatggt aatacttact acggtgtaag tagagattat gcacacggac attacttagt
     7441 agaagctgta attgaaggag aagaaattaa agatgtcagt ataattacag ctaagaatcc
     7501 agaagaagaa gcactagttg gtccaaagaa gcctggtgaa tatgactatg aacctgctgt
     7561 agaaactttt caaaaatggg ctgatatggt tgtagaaaat caatcagtta atgtagatgt
     7621 agtttctgga gcaactcata cagaccatgg tttaaatggt gctttagctc aatgcttaac
     7681 tcaagctggt ttaaatcccg atgattacaa ataattttag atgttagatt aatattatat
     7741 aagcaagggc ccttcttaag ggctcttgtt tatagtatat aaataaagtt aaataaggtt
     7801 gtagataaaa atgaattata aaagattttt ggttgcaatt ataattttaa taataataat
     7861 aggcggtatg tattttattg gaaataataa tccgggtaat cgaaataata cgattgaacc
     7921 aaatggtgat gtaaataaca aggttgaagt aacaaaaaca gatggttcag aatttttaat
     7981 ggataccctt gtcagattaa ggattcatga tgaacagccc cagcagatat tagatgaaac
     8041 atttaacaaa ttgcggtatc tggaaaatat atttagtaaa actattactg aaagtgatgt
     8101 ttccaaaatc aattctaatg ccggtcaaaa agcagtagaa gttagtgatg atacgtataa
     8161 attgttagaa gaatcttatc agtttgcaga aaaaactaat ggtgttttcg atcccacgat
     8221 tggtccttta gtttctttgt ggggaatagg tacagaagat caacaggttc ctactgaaga
     8281 agaaattcaa aacacattaa aaaaagttga ttatagaaag ctagaactta gagatgacaa
     8341 taaggtgttt ttaaaagagg aaggtatgtc tattgattta ggtgcgattg caaagggttt
     8401 tgcagctgat tatgtcttaa attattttaa tgaaaaagat gtagatagtg cttttatcaa
     8461 tatgggaggg aatgtctctg ttcatagaga taaaacagat gatactttat ggagtatcgg
     8521 aattcaggac cctgaccaga cccggggtag tattatagca gcggttgaag gagaagatat
     8581 gtctgtagtt acttccggta attatgaaag atattttaca aaagatggaa ttagatacca
     8641 tcatatactt aatccggaaa ccggttaccc cgcccgcgag ggaataataa gttcaaccgt
     8701 tatttctaat aattctagtt atgcagatgc tttatcaacc tctttttata ttttaggaat
     8761 agagaaatca tttgaaattg caaaatcaat ggaagatatt aatatcgtgt tagttaccga
     8821 agataataaa gcttacgtaa gtaaagaact caaagaccgc attagattta caaccgaaga
     8881 actggaagtt atatatagat aatttcaaag tgaaaatgta ggtgattaag atgttttggg
     8941 atcaataccc cgattttgct gaagaactaa atagttttga aaaatatttt cgcaatcaat
     9001 tatccagtag taatgagttg ataactgagg aaatcaaaag attagctgaa gcaggtggta
     9061 aaagagtaag gcctgcttta ttaatctcag tagctcatct gggtgaatac aaacaagaca
     9121 ggatttggcc gatggctgct gccatagaga tgatccatct tgctacttta atacatgatg
     9181 atgtgataga taagtcatct ttacggagag gtgtcaaaac tacccaccag aaatatacca
     9241 ataaaacggc tatctttatg ggggattatc tatttgctct ttccatgcag attatattag
     9301 aagaattcgg taatgaaaaa atagacgggg ccttattaag acagatgaaa aatacgataa
     9361 agagtatcaa aagtctctgt gaaggggaag tatcccaatt ccaaaggcag tttgaaaaaa
     9421 ttcctacctt taaagaatat ctttctgcta tcaaaaggaa aactgcttta ctttttaaag
     9481 caagtgctat gttaggggca gacctatcag ggatgaaaaa gtatcaagta tttaagatgg
     9541 gtaagttagg gtttaatatg ggaatggctt ttcaaatggt tgatgatctt tttgattttc
     9601 aggaaaaaaa tcaacagtta ggaaagccgc agaagaacga ctttacccag gggatttata
     9661 ctctacctat tatatatata tataatgaag tggaaaataa agaaaaatta gaaaagtttt
     9721 taaaagaacc ggaaaagcat tcagaagaaa taaaaaagct tgtgcatgaa aaaggcggta
     9781 ttaattatac taaaaaactt gcctttcgat ataaggaaaa ggctcagaag ataatcaagc
     9841 cttatactga taataaatat ggtgagatta ttaattggtt aactgaaaaa ataataggaa
     9901 gaaattttta aaacatcggt cacacgatgt ttttttttat atatatttat ggtataataa
     9961 ttttaaaata gaacaacagt tcgctttaag gggaattaga tatgaatata gaacagatga
    10021 aaagtcattt acaaaacaag cctaagttca tgaacagaat tacagcctgg cgggagattg
    10081 agggtaggga tggcaaatac tccgacttac ccgttaactt agaccagaga ttaaaagaag
    10141 ttttaaaaac ccaggatatc gataggttat atagccatca aaaagaagca tataattaca
    10201 taaataatga aaaagatact gttgtagtaa ctccaactgc ctcaggcaaa accctttgtt
    10261 ataatcttcc cgttctgaat tccattttag aagatgataa caacagggct ttatatatgt
    10321 ttcccacaaa agccctatcg caagatcaac tttccggctt atatgattta atcagagctt
    10381 tagatgagga tattaaaacc tatacttatg atggtgatac ccaggtaagt gcccgtaaaa
    10441 gtataagaga gtcgggccat attgtaatta ctaatccaga tatgctgcat gtagggattt
    10501 tgccccacca tacaaaatgg attaagttat tcgagaattt aaagtatgta gtaatagatg
    10561 aggtacacag ttaccggggt gtttttggta gtcatgtagc gaatgttatc agaagattaa
    10621 agaggatctg taacttttat ggttctgatc ctatcttcat ctgcgcttca gctacaatcg
    10681 ataatcccgg tgagttaact tctaaattga taggcaaaga ggtagaagta gtcgatgaaa
    10741 acggagctcc ggccggaccg agagatttca tattctataa cccaccggtt gtaaataaag
    10801 aattaggtat tagaagaagc tatgtattgg agagcaagaa aatagcttct atcttcttaa
    10861 aaaataaaat taagacgata atctttacga gaagcagatt gaggactgaa attatacttt
    10921 cctatttaag agatgataaa ccgcgtgatg tgaatattag aggatatcgg ggcggttatc
    10981 taccgtccga aagacgccag attgaaaaag gactacgaga gggagatata cacggtgtcg
    11041 taagtactaa tgccttagaa ctgggagttg atatcggtca attagatgtc agcatattag
    11101 ctggttatcc cggtactatt gctagttcct ggcagcaggc tgggagagca gggcgcggcc
    11161 agcgacgttc tatttcagtt ctggtagctt ccagtagcgc cttagatcaa tatattgtaa
    11221 ataatcctga atactttttc gaacagccgg ctgaaagtgc cttgattaac cccaataacc
    11281 ttatgatctt actttctcat ataaagtgtg ctgcttttga actgcctttt gaggaggggg
    11341 agcagtttgg ggttgaaact actgatgaaa tacttaatta tctagaggat aataatgttc
    11401 taagatataa aggaggaaaa tggtactggg cagctgaaag gtatcccgct gaagatataa
    11461 gcttaagaag cgcttcctct gatgattttg ctgtaataga tagaaccaaa acaggtagcc
    11521 ctcgggttat cggtagagtt gattacttta gcgttcctac tactattcat gaggatgcta
    11581 tctatataca cgaaggcaag cagtatcagg ttaaagaact cgattatgac ggtaaaaagg
    11641 cctatgtaaa agaagtagat gtaaatttct atacagatgc taatttagcg gttgatttaa
    11701 gtgtgcttga ccaatttgat aaaaaggaac tttcgaaaag aacagctgaa cacggggaag
    11761 tagtggtgag tgcccaagct actatgtata aaaaagttaa gtttaatacc catgaaaatg
    11821 tgggatatgg agatattaac cttccggaag tagaaatgca tacaactgcc tggtggatga
    11881 gcttggataa agaatttgaa gagaagttaa ataagaaagt attagagaat gcgcttttag
    11941 gcctttctaa tcttttagag aatattgcgc ccatatattt gatgtgtgat ccgggcgata
    12001 ttaattcaac agttcagatt agatcaccct ttacgagtaa acctactatc tatatatatg
    12061 atagttatcc aggtggtgtg ggtttaagtg aaaaattata tcgaattgat tataaactat
    12121 tgaaatccgc ctataagtat ctacagaaat gtccctgtga agctggctgt ccggcctgtg
    12181 tcggtcccat aaatgaggtg ggattaaatg gtaaagaaaa gaccctttac ctattaaagg
    12241 aggtattaca gggtgaatct gagggataaa ttaaaaaagt ttgaaaaaaa tcaaaaacct
    12301 cacagatcta aagaagagat agaatataaa aataaagatg cctttaataa tcaattaatt
    12361 agtaataata tgggtactta tttaaagtcc gttaatgatt ataaaaatga atataaacat
    12421 ggtttatacc aaattgatca attgaaggag caattagata ctgaaatatt atttaaaatt
    12481 gccaacaata aatcaaccaa attaaatgat gaaattatag gaaacctcct ctttatagat
    12541 acagaaacta ctggattaat ggggggaacc ggtaccgttt cctttttaat aggagctgga
    12601 tattttacag ctggagattt tgtaattgaa cagtatttaa tgcgtgatta tgatgaagag
    12661 gctgccatgt tacaggattt taaagagata atgggaaatc ataatataat tgtttctttc
    12721 aatggtaaaa gttttgattt acctttgatt aagacccgtt taatcatgaa tagatttgat
    12781 cgacctaatt atgattatca tctagacctt ttacattctg ccagacggct ctggtcattt
    12841 ttagattcct gttccttaaa atcactggaa agaaatattt taaattttga aagaatcgat
    12901 gatgtgcccg gtcatctgat tccaggccta tactttgaat ttctagagaa taaaaaccta
    12961 gaactcttgg cacctgtctt agaacataat atatatgata ttttatcttt agttactctt
    13021 tttacccatt taaaagagat ccacctagat aatgtggata atttggactc aaatgagctt
    13081 tttcatttgg gtagaataaa ggaaaaagaa aaaaactacc atgattgcat tgactatctt
    13141 gaagcttcgg ttgaaaattc agaagaaagt actttaaagt atagggcatt aaaaaagtta
    13201 agttggcagt ataagcggat agatagatat gataaagcag cggaaatatg gaaagagatg
    13261 attgatcaaa aaaagcaggg gatattccct tatgttgaga tggcaaagta tttagaacat
    13321 aaaaagaaaa actttaaaga agctttaaac tacactaatc aggcaataga aatgttgaat
    13381 gaaagaagaa ttattattaa taactttgat aaaaagataa aaaatttaga acatcgcaaa
    13441 gaaagattga taaaaaaatc aagtaaaatt taatatattt tttaattata ttcaaaaata
    13501 tgataaaata taaaaaagcc cttaggagtg agtatattgg aagaaaaaag agtaaataca
    13561 ttttctatag ttggatatga tcctgataaa gaagaattag gaattgcggt tcaatctaaa
    13621 tttttagccg taggggcagt tgtaccatgg gcaaaagcca aagtaggagc aatagcaacc
    13681 caggccctgg caaatttaga ttacggagaa gaaggactta aacttttaga ggaaggttat
    13741 tctcctgcag aagtaataaa gaaattaagg gcggaagatg aaaacgatca aagtagacaa
    13801 gtcggagtag tttcagctac tggtaagtct gcttcattta caggtgatga ttgttatgat
    13861 tgggccggtg gtataaccgg agataatttt gcctgtcagg gaaatatttt agttggcgaa
    13921 gataccgtgc aggccatggc cgatactttt actaacactg agggggattt agctgataga
    13981 ttggtcactg ctttagaagc tggtcaaaaa gcgggcggtg ataagagagg tagacagtcc
    14041 gccgctttat tagtagtaaa ggaaaatggt agttacggcg gctataacga tagatacata
    14101 gatttaagag tagatgatca tgaaactcct atccaggagt taaaaagact tttagatata
    14161 ttttatttat attttggtga taaggaagtt gaaaaagtag agttgacaga tgaagtcata
    14221 aaagaagtta aacagtatct taaaaatttg gatttatttt ctggaaaaat agatgcggaa
    14281 aataatgagg agttcaaaaa atctttagaa tctttttatc atcaagagaa ctttgaagag
    14341 agaaaacaaa aagaaggttt cctagacaaa gatatattaa attacatgaa ggacaaggca
    14401 gaataaataa cggaggtgca tattaaatgg atcgtaaaga atttattatt gaaattgatg
    14461 ggatgtcaga cagaaagtta aggcagttat taaactttgt ttatgaggaa tttgaaaaag
    14521 aggataaagt agatctactt aaggataaaa ttgacgaaat aaagaaattt agttaataac
    14581 agataaattt ttatattaaa taagaataga attttttact gtaaaattat ttaatataat
    14641 acgtaataaa acaactaatt tttttatatt tagaccctga aattaataag caaataaata
    14701 aagaaaatag taactgtttt acttgacatt atgaggttct tgtgctaaga ttattaatgt
    14761 ttgacactat gcgaaagtgg cggaattggc agacgcgcta gatttaggat ctagtgggct
    14821 ttacccgtgg gggttcgagt cccccctttc gcaccattta ttcagttaaa cattgacctt
    14881 tgccttaaac cccgaattgg cctttgccat atcggggctt attgacattg tgatgaaaaa
    14941 taatataatt gtgttttatg aaaggatgat taagcagtgg aagtaacaaa aaatcaactt
    15001 gaaggaaata aagttgaact gaaagtagaa atagaagctg aacgagttaa tgaagcttta
    15061 gaacaagcct ataagaaagt tgttaaagat attgatattg atggatttag aaagggtaaa
    15121 gtacctcgga gagttttaga agctagatat ggtaaagaaa ttcttcataa agatgcttta
    15181 gacattttga ttccagaagg atatcgtgaa gctttagaag aaacaggtat agaacctata
    15241 gatcaacctg acattgaaga ttatcatatt gcagaagatg aacccgccag ctttacagct
    15301 gtagtggaag taaaacccga ggttgaattg ggcgaatata aagaattagg tattgaaaaa
    15361 gagagtgccg aagtttctga agaagaaatt caagaagaaa ttgataaagt tagaaaccaa
    15421 catagtcaat tagtagcctc tgataaagaa gttgtagaag atggcgattt tgtaataatt
    15481 gactttgaag gtaaaaaaga tggcgaacca tttccggggg gaagtgcaga agaatatagt
    15541 ttagagattg gttctaatac ctttattccc ggttttgaag aacagttaat cggagccaaa
    15601 gttggcgatg aacttgaatt aaacattacc ttcccagaag attataatgc gaaggactta
    15661 gcgggagaag aggtagtgtt tgatgttgag atcaaagaat taaaagaaaa agagctgccc
    15721 gaattagatg atgagtttgc caaagaagtt agtgattatg aaacatttga agaatataaa
    15781 gaaagcatca aagaaagatt gcaggaaaac aaagaagaac ggactgaaag agaatatgaa
    15841 aacaagttaa tcgagaaagc atctgaaaat gctattgttg acgtacccga aaagatggta
    15901 gaagatgaat taaataatat gttccagaac tttgctcaaa gtgtttcaca gcagggcatg
    15961 gaggtagagg attatttaga ttatatggga acagatgaag aaggctggaa agaacaaaat
    16021 aaagaagcag ccgaaaatag aaccagaagc aacctggtct tagaggctat tgctgaacag
    16081 gaagatatag aagtaagtga agaagagatt gaagaacaaa ttcaagaaat tgcggaaagt
    16141 aacgaccagg accccgaaca aatcaaagca tttttacaaa tgcagggaca attagatgga
    16201 ctcaaagatg gtttgcagat gcaaaaaact attgaatatc tgaaagaaaa taactaaaaa
    16261 atatataaat tgagggggaa taatgatgag tggtttaatc cctatggtag tggaacaaac
    16321 taaccgtgga gaacgttctt atgatatcta ttcaaggcta ttaaaagata gaatagtatt
    16381 tttaggaagt catgttactg atgatatagc gaatttgatc attgctcaat tgttattttt
    16441 agaagctgat gatcctgata aagatattta tctgtatata aatagcccgg gaggttctgt
    16501 aacagcagcc cttgctattt atgatactat gcagtatatt aaacctgatg ttgttactat
    16561 ttgtatggga caggcagcta gtgctggtgc cttactattg gcagcaggtg aagatggcaa
    16621 aagatatgga ctaccctatg cacgtgttat gattcaccaa ccggccggcg gagttcaagg
    16681 caaggcgaca gaagcagaag tccatattaa agagttaatg agaattaaag atctattaaa
    16741 tgatatctta agtgatcaca ctggacaacc ggtagaaaaa attagtgaag atgttgaaaa
    16801 agattacttc atgacctctg aacaggcggt tgaatatggc attattgaca aagtaatatc
    16861 tagaagtgag atgaaagaca ataaagaaga gtgatattag aggtgattaa atgtttaaat
    16921 ttgatgatga agatggacaa ttagaatgtt cattttgcgg aaaatcacaa gatgaagtaa
    16981 aaaaattaat agcaggtcca ggtgtatata tttgtaatga atgtgtagat ctatgtaacg
    17041 atatattaaa agaagaacta agtgttgatt tggaccttaa ttttagtaat ataccaaaac
    17101 caagagaaat caaagatatt ttagaccaat atgttattgg ccaggatcgg gccaaaaaga
    17161 ctttatctgt ggcagtatat aaccattata aaagaataaa ttctgatatg aaaatagatg
    17221 aagtagaact acaaaagagt aatatcttaa tggtaggtcc aaccggctgt ggtaaaacac
    17281 ttttagcaca aactttagcc agaattttgg atgttccatt tgctattact gatgctactt
    17341 ccctaactga agcaggttat gtaggagaag atgtagaaaa tatcttatta aaactaatac
    17401 aagctgcaga ttatgatatt gaaaaagcag aaaaaggaat tatttatgtt gatgaaattg
    17461 ataaattagc ccgcaaatct gagaaccctt ctattacaag agatgtttct ggtgagggag
    17521 ttcagcaggc acttttaaag attatagaag gaactactgc aaatgttcca cctaagggtg
    17581 gcagaaaaca tccccatcag cagtttatcc agattgatac gaccaatata ttatttgtct
    17641 ttgggggagc tttcgatggt ttagaaaagg ttatcaaatc aagaatctct gaaaaagtaa
    17701 tgggttttgg agccgatatt aagacaaaag atgaaaaaga aaatatcggt gatattttaa
    17761 aacatgtctt acctcaggat ttattacatt atggacttat tcctgaattt attggtagga
    17821 tgcccgttat agctacactt gacgaattaa gtgttgatga cctggttcaa atcttaacag
    17881 aacccaaaaa cgcccttacc aagcagtata agaagttctt tgaaatggac catgtagatc
    17941 tagaatttac agaaggtgcc ttaagagcga ttgctagaaa gacactggaa gtaaatacag
    18001 gagctagagg gttaagatca gtggtggaag acacgatctt ggaaatcatg tatgacttac
    18061 cctctcaaga tgatataact aaatgtatta tcgatgaagg ggttattgaa aaaagtaaag
    18121 aaccgaaatt aattcgaaag agtgatgtcg gtagcgatag agaagaaagt gcataagaac
    18181 ttaacagaga taataatgag atctcattat tatctctttt tttactttgt atattattta
    18241 ttttcataaa tgattgagat ataatataat aaggacaaga agtgaaacaa actataacta
    18301 tatattataa agtgaatatt atagtgagtg aggtgaaagt cgtgaaaaaa gatttagaag
    18361 aagttaaaat tagtgatgga gatatagaaa aaatagatga tgattttatt gaactacctc
    18421 ttttagcatc aaggggtgta gtcgtatttc ctcacatggt tatcccttta ctagtgggga
    18481 gagataaatc ggtagaggca ttagaaaaag cgatgttaga agaaaaaaga atattaatag
    18541 cggcccaaaa agatgagtca attgaagaac cagtagtaga ggacttatat aatgtgggta
    18601 ctattactga agtcaaacaa ttagttaaac ttccaaatgg aatgattaag gtaattgtgg
    18661 aaggaattca gagggctcaa atcaaggaat atctaaaaac agatgaatat ttcaaggtta
    18721 aggcagaggt atttaatgag gaagctgaac agtatataga agaagattat gaaatagaag
    18781 ctttgatgag atctgttgtg gattacttta aagaatatgt gaaatataat cgcaacttcc
    18841 cggccgaaac aatggtttcg gtaatcaata ttgaagagcc gggtcggtta gctgatatca
    18901 tagcctctaa tcttgagctc aagtataaaa aagaacagga acttctagaa gctttttctc
    18961 ataaagaaag attagaactg ctgatgaaaa taatcaaaaa agaaatagag atattaaaga
    19021 tagaaaatga tattaatgaa aaagtcaaag accaggtaga aaaaacacaa aaggaatatt
    19081 ttttaaaaga aaaattaaag gttataaaag aagaacttga tgaagaagat atggatgaga
    19141 tagaagaata tagagctcaa atgcaggaga ttgattttcc cgaagaagtg gaagaaaaga
    19201 tggaaaaaga gattaaaaaa ttaaataaaa ctccttccat gtcgcctgaa tctacagtaa
    19261 ttaggaacta tttagactgt atgattgact taccctggga tgaagaaaaa aatactgaaa
    19321 ttaatataaa agaagctgaa aaaatactag aagaagacca ctacggttta gaagatgtaa
    19381 aagaaagaat tattgaatac ctagccgtgc gtaaaatatc taaaaagaaa aaaagcccta
    19441 ttctttgtct agtaggagca cctggtgtcg ggaaaacctc tttaggacgt tctgtcgccc
    19501 gtgcattaga aagagatttt gtcagaatgt cattaggtgg ggttagagac gaagctgaaa
    19561 taaggggcca tagaagaact tatatcggct cccggccagg caggattatt aattcaatgc
    19621 gagatgccgg cagtaaaaat ccaatattct tattagatga agtcgataag atgtcggccg
    19681 actttagagg tgatcctgct tctgctttat tagaagtatt ggatcctgaa caaaacagtg
    19741 aatttacaga tcattattta gaagttcctt ttgatctatc ggatgtcttg tttattacta
    19801 cagccaatgt aaccagtacc atccctgctc cacttttaga taggatggaa gttatagaat
    19861 tacccggcta tactgaagat gaaaaggtaa agattgctga gcgtcatctg attccacgta
    19921 ttttaaagga acatggattg gaaaaagctc agattaatat ttcagataat gccttatata
    19981 aaataattag agactataca agagaggccg gcgtaagaaa tctggaaaga gagatttctg
    20041 ccatcgtaag aaagatcagt aaagaaatag tagaaggcag agataaaaag gctagaatta
    20101 caaaaagaaa tgttgataaa tatctaggtg tacgtaaatt taagtatgaa gttgccaaaa
    20161 aagaagatag agtgggagtt gccaccggtt tagcctataa tcaagcaggt ggagatatcc
    20221 ttgatatcga ggtggcagta gtgccaggta aaggtgaagt cactttaacc ggttccttag
    20281 gtgatgtaat gcaggaatct gctaaagctg ctttaagtta tataagatct aaatataaag
    20341 aattgaacct tgaaaaggat tttcacgaga actatgatct gcatattcat gtgcctcaag
    20401 gtgcggttcc caaagacgga ccatcggctg gtattacaat tgcacttgca attgcatctg
    20461 ctcttaccaa cagaaaagta aaaggagatt atgccatgac cggcgaaatc tcactacgag
    20521 gtagagtctt accggttgga ggaattaaga ccaagatttt agcagccaaa agagctggta
    20581 taaataagat agtcttacca aaagaaaatg aagaatcaat ggaagagatc caaacttatc
    20641 taaaaaaaga tctagaaatc aactatgttg agcatataga tgaagtcatt gatttattgt
    20701 tattggagga ataatgagtg aaaataaaca atcctgaatt tgttaaaagt gcgcatacaa
    20761 ttgaggatta tccccataat aatctcccgg agatcgcatt cgccgggaga tctaatgtgg
    20821 ggaaatcttc catgataaat aaggtgttaa accgtaaagg gttagccaga ataagttcca
    20881 aaccaggtca tactagaagc ataaattttt ataatattga taataatttt tatctggttg
    20941 atttaccggg ttatggtttt gcaaaagtgt ctaaagaaga aaaagataag tggagtatgc
    21001 taattgatga ctatttacat tatcgtgaaa atttagcagg cttagttatg attgttgatt
    21061 ccagacataa acccactgaa caggataaag ttatgttaaa ttggattaga gaagtgaaca
    21121 ttccaaaaat aatcactgca actaaagctg ataaaatttc taataataaa aaagcaaaac
    21181 aaaagaaggt tattaaagac gttcttgatt taagagcgga tgaaaggttt acattttttt
    21241 ctgccaaaac aggagaaggg aaaaaggata tcttttcatt tatcaaccat attattaagt
    21301 agaggtgtag cgatggaatc tcaatcatat acaagttcat ttgcggttat atatgatgat
    21361 gtaatgcaga gagtgcctta ttattattgg tataagtatt taaaacatct cttagcctat
    21421 tatgataaga aaccggaaaa gataatggaa cttgcctgtg gtacaggtaa tatgatcaaa
    21481 tattttgcca aagaagctga ttctatatat ggcatagata aatctaagga tatgctgaca
    21541 gttgctcaaa ataaattttc tgagaatgat aatgtagaat tattcaatac tgatatgagc
    21601 gaaaaatata aatacggaga atttgatctt atttattcga tcttcgatag cctaaattat
    21661 attttagagt atgaagattt attaagagtt tttgcaaatg ctcactataa tcttaatcaa
    21721 gatggaatat ttatctttga tatgaataca atataccgtt taatggatat tagtgaaggt
    21781 acaaataaaa ttgagggtga tgattacacc tgttattggc gggatattgt agatcgtgaa
    21841 aaaaaagaat ggattgtaga attaaatatc tatctagacc gcggtgaaaa tattgaaaat
    21901 tttactgaaa aacatgttga gactagttat tcattaaaga agattaaaga agctctttta
    21961 gaagctggtt ttaaacatgt agattattac aagtctttta cctttcggtc tggtaaaccc
    22021 caaaataata gaattcattt cgctgctatg aagaagaagc ccgaaagcaa atctattaaa
    22081 aattttctaa tcaagctcaa atggaattta attagtcctt ttatttctag tttttaatat
    22141 taaaaattgc ttgacaccta ttttatctag taatatacta atttaaaagt ggggctatag
    22201 ctcagtagga tagagcaaca gattcctaat ctgtaggcca caggttcgaa tcctgttagc
    22261 cctaccatta ttttaaattg aataaataaa tgcttcctat ttcccagtct aaagatgctg
    22321 ggtttttatt tttttagaaa gatatttttc aaaatgctat atagatatag aagaaggtaa
    22381 ttatttactt atgcataagt ataaaaatag agacaggtta gatattattt atgataggat
    22441 ctacaaataa ttaaacttct tttgataaaa aaattattct aataatcaca cctcataatg
    22501 tttacgctca agtaagaggg gggtgatttt atttatttaa aaaaatattc atttaaaatt
    22561 ttattagttt tattaatctt agtattagtc aattcagttg gtttagctca agatgacttt
    22621 gaagagttaa aaataccgac tgaagtacaa gacctgataa ataattttgc caagattgaa
    22681 tatgatatta ttacctttaa tgaaaatggc gaaaaaaatg ttgattctaa tatgaaatat
    22741 gtttatctag gaaaagaaga ggttaatggg actgaaacag ataaaatatc aattgaaatt
    22801 gcagacaaaa ttcaagaaga tacaccttcc aaattatatt tttggttcgc tgatgataat
    22861 atcaaacaaa tgcagattga cgagcaagta ttatcagaaa atatggccca aatgatggca
    22921 gataggtttc taagtgctat tttttctccc ctcaaacaat tatctcaata tgatatagca
    22981 gaatttagaa agataggaaa agtaacaaaa tataaagaaa aatattcagg gcgagaatta
    23041 aacataacaa aaattgaagt tgaagatata cctgaatatg aattagagta cggtatgact
    23101 gagattgcag aattagaaga aactgcagtt gttttagaat atcaatatat atcttccgaa
    23161 aacaagaata aagttgaatt taaagttaaa gactttcaat tacattaaat gatttcaaat
    23221 ccctattgag aagatgaaag tgagttattc tatactgatt atggggttgc cagattcaat
    23281 tattattgac ttttcaactg ttatagatta gaataaataa tggtggaaat gattactcaa
    23341 cttaaaataa taattttaag tttatacaat tatattctac catttaaaac attcacccag
    23401 tatgagaata ctgggcattt ttttatatat ttgatatata attaactcta caggagggaa
    23461 tttcatgcca aaaattgctt ttatgggggc gggcagcaca atatttgcaa aaaatgtaat
    23521 aggtgactgc ttattaactc cggccttaag agaatctgaa tttgctttat atgatattga
    23581 tcataagaga cttaaggaat cagccatgat gttaaaaaat attaaccata attatggaaa
    23641 ccatggtact attaaggctt acaaggaccg caaaaaggct ttaaaaaatg cagattacat
    23701 aattaatgca attcaggtag gaggatataa accatccacg gtaatagatt ttgagatacc
    23761 aaaaaaatat ggattacgcc aaaccatagg tgatacaata ggtattggag gtatttttag
    23821 ggctttaaga actattccag taatgcttga ttttgcgaga gatatacaag atgttgcccc
    23881 tgaggcttta tttctaaatt ataccaaccc gatggcaatt ttaacaggtg ctatgttaaa
    23941 agcaacagag gtaaatacag ttggactttg ccatagtgta caggactgtc taaattctct
    24001 gttggaacca ttaggtatga gtacagaaaa tgtcaggcat aaaattgcag ggattaacca
    24061 tatggcctgg ctattagaag taagtagaga tggaaaagac ctttatcctg aaattaaaaa
    24121 gaaagctttt aatagagaaa aaactcatga tgatagggta agatatgaaa ttatgaagag
    24181 gtttggatat tatgtaacag agtccagtga acataacgct gaatatctgc cttattttat
    24241 taaatcggag tatccagaac ttattgagaa gtataatatt cctttagatg aatatccacg
    24301 tcggtgtgaa gagcaaattg ccgaatggga agagatgaga gatagtttgg tagacaatgc
    24361 agacttagaa catgaacttt ctaatgaata cgcttcttac ataatggaag ccattgaaac
    24421 agaccaacct tataggattg gtggaaatgt agttaataca aaattaatag aaaatttacc
    24481 agaagaagca attgtagaag taccctgttt agttgataga aatggaataa ctccctgtca
    24541 tgttgggaga ttacccgaac aattagcggc tttaaacagg acaaatatta atgtccatat
    24601 catgacaata aaagcagcct tagaaaagaa aagagagtat atataccagg cagctttact
    24661 ggatcctcat acttcttctg agttatcaat agaagatact ataaatctat gtgatgatct
    24721 aatagaggcc catggagact ggcttccgaa atatagttaa aagaatatta tcaaagtaat
    24781 aaaaaaggat ataaatcgaa gattattctg gtttatatcc ttttaaaata aaatactccc
    24841 atagataact atcactatct aataagttat ttgtttttaa ttggaaataa tgaagtgtat
    24901 ctttggttct aatagtttca atgatatcat catttcttct aattgaaaat tcttgaagag
    24961 gtttatagcc tgttatctta tagtaatatt tttctttttt gtatacctgt tttactttat
    25021 ctaaatattt aaaatcatca gtagttttaa taaagtaaag
//
LOCUS       DATGCJ010000239         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_260577, whole genome shotgun sequence.
ACCESSION   DATGCJ010000239 DATGCJ010000000
VERSION     DATGCJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260577"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..139
                     /locus_tag="VKN64_10655"
     tRNA            63..139
                     /locus_tag="VKN64_10655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97..99,aa:Met,seq:cat)
     gene            204..280
                     /locus_tag="VKN64_10660"
     tRNA            204..280
                     /locus_tag="VKN64_10660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Gly,seq:tcc)
     gene            332..408
                     /locus_tag="VKN64_10665"
     tRNA            332..408
                     /locus_tag="VKN64_10665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Met,seq:cat)
     gene            410..504
                     /locus_tag="VKN64_10670"
     tRNA            410..504
                     /locus_tag="VKN64_10670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ser,seq:gct)
     gene            514..589
                     /locus_tag="VKN64_10675"
     tRNA            514..589
                     /locus_tag="VKN64_10675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Glu,seq:ttc)
     gene            591..666
                     /locus_tag="VKN64_10680"
     tRNA            591..666
                     /locus_tag="VKN64_10680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:624..626,aa:Val,seq:tac)
     gene            675..751
                     /locus_tag="VKN64_10685"
     tRNA            675..751
                     /locus_tag="VKN64_10685"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:709..711,aa:Asp,seq:gtc)
     gene            782..858
                     /locus_tag="VKN64_10690"
     tRNA            782..858
                     /locus_tag="VKN64_10690"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Thr,seq:ggt)
     gene            869..944
                     /locus_tag="VKN64_10695"
     tRNA            869..944
                     /locus_tag="VKN64_10695"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:902..904,aa:Ala,seq:ggc)
     gene            complement(1018..2172)
                     /locus_tag="VKN64_10700"
     CDS             complement(1018..2172)
                     /locus_tag="VKN64_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMA60744.1"
                     /translation="MASLYHRGPKTWRISIELGKDPKTGKRMRKFKTFKGTKKEAQKK
                     MLELVKKYEKGYFSEAENITVKEYLIKWLYEYKKNDLSRTTFIDYKNIIDNHLAPYLG
                     ELKLKDIEERHIIKYQNDKLQNGRMDGNGGLSKRSVQYHHRVLSQALKHAVYPYKLIE
                     NNPCRSINAPSPKNTEFFPLSQNQANKLLDSLKNNLSFYTLIYLALYTGLRRGELLAL
                     RWKDIDLDNKRLFVRRSVTEISGEGLIYKEPKNENSKRAVDFDEDVANILNYYRKKQF
                     KYYKNDAIIFLHEDGSVIRPGYVTKKYKKVVKKLGLNKTRFHDLRHTHASWLLQLGEN
                     PKVVQERLGHHDVSITLDIYSHVIPSMQKDAVKKLKKNKNRNHGYKMDTK"
     gene            complement(2344..2610)
                     /locus_tag="VKN64_10705"
     CDS             complement(2344..2610)
                     /locus_tag="VKN64_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60745.1"
                     /translation="MLTEEDIVPLEVFDNDLKIELFLQLEDLDKEIWGFVYLNTSGNY
                     LIVMNENLKGKLLREVYDKSIKIIDCDFSFLDYMLYIITKDNLK"
     gene            complement(2644..3036)
                     /locus_tag="VKN64_10710"
     CDS             complement(2644..3036)
                     /locus_tag="VKN64_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60746.1"
                     /translation="MADFKNRLKELRKEKNLKQSEVAEKIELGRSTYANYEQGKRFPG
                     KENLIALADFFEVSIDYLLGKSDNRQSAEKIINKIYDDQELLDFYQSIKENEHLKKLF
                     NNSKQLSPESIKQIIKIIKTFEESNNNT"
     gene            3197..3412
                     /locus_tag="VKN64_10715"
     CDS             3197..3412
                     /locus_tag="VKN64_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA60747.1"
                     /translation="MNRNRAKLKQLRINNELFQKDIANLLDISTSFYSSIERGKRNPT
                     LSLAKKTADLFGESIENIFYNNTKLEE"
     gene            3415..3741
                     /locus_tag="VKN64_10720"
     CDS             3415..3741
                     /locus_tag="VKN64_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA60748.1"
                     /translation="MTVLEKILMELQNLNSKIEKIETSESNNYPKLLNGKANLNSTEA
                     MELLGIGRNKLLELVHSNAIPHIANGSKYLFPVDQLFNWIKYKASENYKTEEAADIDK
                     VFKSIS"
     gene            3815..4336
                     /locus_tag="VKN64_10725"
     CDS             3815..4336
                     /locus_tag="VKN64_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60749.1"
                     /translation="MNNIGLLLKKERKRRGMTQKQLADLMPGVTRSSISKYESSGNIP
                     SYNLEIIIEVLKSPRLKLAAKGTVINSIFLDQVDLSPLATQQKMIEELEETLESLKGI
                     SLINKLNAEDFNERERSKLFKEVLTQICDLDICISLFMISMAENFDFDISRLEEHEIN
                     KMKQKGYVSRGLY"
     gene            4479..4637
                     /locus_tag="VKN64_10730"
     CDS             4479..4637
                     /locus_tag="VKN64_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60750.1"
                     /translation="MIRYMDLAARIVIGFGYLLVGYVIWKEGKKIMAERKKDDTKEKT
                     QLSKQLSS"
BASE COUNT         1546 a          775 c          791 g         1532 t
ORIGIN      
        1 agagcacctg acttttaatc aggttgtcgg aggttcgatt cctccatggc tcaccattat
       61 agcgcggggt ggagcaatct ggtagctcgt caggctcata acctgaaggt tgtcggttcg
      121 aatccggccc ccgcaaccaa attaaaataa atttgcaata atatagatta ttttattata
      181 ataataagtg aattattaaa ctggcgggag tagctcagtt ggctagagca tcagccttcc
      241 aagctgaggg tcgcgggttc gagtcccgtt tcccgctcca tataataaaa tataatgtca
      301 aaaatattca ttatttcatt tatagtttaa agggtctata gctcagttgg ctagagcatg
      361 tggttcatac ccacagtgtc agaggttcga atcctcttag acccaccaag gagagatacc
      421 caagtggctg aaggggcggg tttgctaaac ctgtagtagg gttataatac cctagcgagg
      481 gttcgaatcc ctctctctcc gccattttta aatgccctca tcgtctaggg gtctaggata
      541 ccaggttttc atcctggcgg caggggttcg aatccccttg agggtgccaa gggcgaatag
      601 ctcagttggg agagcacctg ccttacaagc agggggtcac aggttcaagc cctgtttcgc
      661 ccaccatata tcacggaggt gtagcgaagt ggtttaacgc gccggcctgt cacgccggag
      721 atcgcgggtt caaatcccgt cgcctccgcc attttaattg tatgtttata aaaaatatat
      781 tgccgatgta gctcaggcgg tagcagcgct tccatggtaa ggaagaggtc accggttcga
      841 ttccggtcat cggctccatt tagatactgg ggatatagct cagctgggag agcgcttgac
      901 tggcagtcaa gaggtcaggg gttcgaatcc ccttatctcc accagtttaa atcggtttaa
      961 tcattgatat aacaaggata atagaaataa cccagcaatt aaatcgctgg gatattttta
     1021 ttttgtatcc attttgtatc catgatttct atttttattt tttttgagtt tcttaacagc
     1081 atctttttgc atagagggaa ttacatgtga atatatatct agagttatag aaacatcatg
     1141 atgacctagt ctttcttgaa caacttttgg attttctcct aattgcaaaa gccaggatgc
     1201 atgtgtatgc cttaaatcat gaaatcttgt tttgtttaac cctaattttt tcactacttt
     1261 tttatatttc ttcgttacat aacctggtct gataaccgaa ccatcctcgt gtaagaatat
     1321 aatcgcatca tttttgtaat atttaaattg ttttttccga taataattta aaatatttgc
     1381 tacatcttca tcgaaatcaa cagctctttt actattttca ttttttggtt ctttataaat
     1441 taatccttcc ccactaattt cagtaacaga tcttcttacg aagagtcttt tgttatctaa
     1501 atcaatatct ttccatctga gtgcaagtaa ttctcctctt cttagtcctg tatataaggc
     1561 aagatatatt aatgtataaa aacttaaatt attttttaat gaatctagca atttatttgc
     1621 ttgattttgg gataaaggaa aaaattcagt gtttttaggt gatggtgcat ttatacttct
     1681 acaagggtta ttctctatta atttgtaggg gtagactgcg tgttttaaag cttgtgataa
     1741 tactctatga tgatattgta cagatctttt ggacaacccg ccatttccat ccatgcgacc
     1801 attttgaagt ttgtcattct ggtattttat aatatgtctt tcttcaatgt cttttaattt
     1861 tagttcacct agatagggtg ctaaatggtt gtcaatgata ttcttatagt caataaaggt
     1921 agtcctagac aaatcatttt ttttgtattc atatagccat tttattaaat actcttttac
     1981 tgtaatgttt tcagcttcgc taaaatagcc cttttcatat tttttaacca actccagcat
     2041 ctttttttgt gcttcttttt tagttccttt aaatgtttta aatttacgca ttctcttacc
     2101 tgttttcgga tcttttccta actcaataga aattctccat gtttttggac ccctatgata
     2161 aagtgaagcc ataaaatata cctcctttct cacatattat tcaataatgt acaaaaccct
     2221 acacactttg aacaaaaata tcaaaatata gtacatatgt tcgctttttg gcaaaaaaaa
     2281 tatacttacc ttaataagca ctaatatttc tacggtatta aattgtaata taactatact
     2341 aaatcatttt aaattatctt ttgtaataat ataaagcatg taatctaaaa aagaaaaatc
     2401 acaatcgata atctttattg atttatcata tacctccctt aataatttac cctttaaatt
     2461 ttcattcatt acaatcaagt aattacctga tgtatttaga taaacaaatc cccagatttc
     2521 cttatccaga tcttcaagtt gcaaaaagag ttcgatcttt aaatcattgt caaaaacttc
     2581 tagaggaact atatcttctt cagttaacaa ttaattcact ccttaccccc caattagaca
     2641 agattaggta ttattattag attcttcaaa tgttttgata atcttaatta tctgctttat
     2701 actttcgggc gataactgct ttgaattatt aaataatttt tttaggtgtt cgttttcttt
     2761 tatagattga taaaagtcaa gtagttcttg atcatcataa attttattta ttattttttc
     2821 agcagattgt ctattatcac ttttccctaa gagatagtca atggagactt caaaaaaatc
     2881 agcaagagca attagatttt ctttgccggg aaatctttta ccctgttcat agtttgcata
     2941 agtagatcta cctaattcta tcttctcagc tacttctgac tgttttaaat ttttttcttt
     3001 cctaagttct tttaatctgt ttttaaagtc agccacaacg atacctcctc tacaaaatat
     3061 attataccac atattgagga ataaataaaa taattctcca atctgaagaa aaaaatgtca
     3121 aaagccttga caatctcctt attgtgtcat ataatatagg tagtgggaca caaaacggac
     3181 aataaggagg agaaaaatga atcgaaaccg agccaaacta aaacaactta gaattaataa
     3241 cgaactcttt caaaaagata tagctaatct gttagatatt tctacttcat tttattcttc
     3301 aattgaaaga ggtaaaagga acccaacatt aagcctggct aaaaagactg ccgatctttt
     3361 tggagaatcg attgaaaaca tcttttataa taatactaaa ttggaggaat aaagatgaca
     3421 gtattagaaa aaatattaat ggaacttcaa aatttaaata gtaagatcga aaaaatagaa
     3481 acatcagaga gtaataatta cccgaaattg ttaaatggca aagctaatct aaattcgact
     3541 gaagcaatgg aattactagg gatcggccga aataaattac tagaattagt tcattctaac
     3601 gcaataccgc atattgcaaa tggttcaaaa tacttatttc cagttgacca attatttaac
     3661 tggataaaat ataaagcatc tgaaaattat aaaactgaag aagcagcaga tatagataaa
     3721 gtgtttaaat ctatatctta atacaaggat atcatatttt ttttatcaat gtaaccataa
     3781 aatgtaataa aaggaaaaca aaggaggtga tttagtgaac aatattggct tacttctaaa
     3841 aaaagaaaga aagagaaggg gaatgactca aaagcagctt gcagatctta tgcctggagt
     3901 tacaaggtca tcaatatcaa aatacgaatc aagcggtaat ataccttcat ataacttgga
     3961 aataataatt gaagtactaa aaagtccccg cttaaaatta gctgcaaaag gcactgtaat
     4021 taacagtata tttctggacc aagttgattt gagtcctctt gccacacagc agaaaatgat
     4081 tgaggagtta gaagagacct tagaatcttt aaaaggaata agtttaatta acaaattaaa
     4141 tgcagaagat ttcaatgaac gggaaagaag taaacttttt aaagaagtac taacccagat
     4201 ttgcgatcta gatatctgca taagtttatt tatgatatcg atggcagaaa actttgattt
     4261 tgatatcagc cggctggaag aacacgagat taacaagatg aaacaaaaag gatatgtatc
     4321 aagggggtta tactaatcga taacaaaaag aagacattct gtaaagactg taaaaaggat
     4381 cctgtgaagg tgtggtaaaa acccaataga atgtatgaaa gctgaagagt caagattata
     4441 tttcaaagta tatgatgaag gactggggtg gacgacaagt gattagatac atggatttag
     4501 cagcgagaat agtaataggg tttggatatt tattagtcgg gtatgtgatc tggaaggaag
     4561 gaaaaaagat aatggctgag aggaaaaagg atgacacaaa agaaaaaact cagctgagta
     4621 aacagctgag tagttaaata aatc
//
LOCUS       DATGCJ010000240         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_261102, whole genome shotgun sequence.
ACCESSION   DATGCJ010000240 DATGCJ010000000
VERSION     DATGCJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261102"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VKN64_10735"
     CDS             <1..512
                     /locus_tag="VKN64_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMA60751.1"
                     /translation="AAYKLSKGSSINEEVITFHFLEDVLGWVSILIVGIIYMFKKIPI
                     LDPILSILITSYILYNVFNNFKKILKIFLQAVPSDLSIEEIEKLVIEKTEVITVHHTH
                     LWSLDGENNLLSTHIVVEDELDNSEINNIKEKIRNLLLEHNINHVTIEVDYRSEVCDD
                     EECNLNGNY"
     gene            534..1034
                     /locus_tag="VKN64_10740"
     CDS             534..1034
                     /locus_tag="VKN64_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60752.1"
                     /translation="MQKGSLKGYLFRFSLVHVIIYVFIALLFRNYLNYNEQFTSSVLY
                     NNFRNIDSTIMRFAPLFQLGRGLFLGLIIYPVYNCIIGVKYGWLRLFALLWGLSLIGS
                     VAATPGSIEGFIYTKTPIIEHLLGLPEVTLQTMLFSLIFVSWEKRRFNKRLQRKEIIE
                     ESTKEE"
     gene            1039..1587
                     /locus_tag="VKN64_10745"
     CDS             1039..1587
                     /locus_tag="VKN64_10745"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60753.1"
                     /translation="MRYFKKLVGENIYLSPINLEDYEKYTEWINDLDISLKLGNAHQI
                     YTLQKEQEILEDISENSFAIILKENDKLIGNCGLIDVEQVTRKAELGIFIGEKEYWNM
                     GYGTEAITLLLDYGFNLLNLHNIMLEVFAFNKRAIECYKKIGFKEIGRRREAREIAGK
                     KYDEVFMDILATEFTEGKISLE"
     gene            1595..2923
                     /locus_tag="VKN64_10750"
     CDS             1595..2923
                     /locus_tag="VKN64_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMA60754.1"
                     /translation="MVKSNLHTVLTFILFITLFFSLNVKAVDEMFIGNWQGFISVQEQ
                     KLNISVTFEQVGSELKGTIDIPQQNLVDYPLDIREVNDNKIVFAMNNIPGDPVFEGTL
                     NNNKITGTFKQSNNKLQFNLSRTQNLEGKNDEINLKGSEREIAIPVEGGNIYGSLTYP
                     KVHDINDPIAIIVAGSGPTNRNGNTPLIEQKINNLKDIAHYLANNGILSIRCDKRGVG
                     KSSYLVDSKTPTFSQYREDILQVIEYIKSNLGREEDQIFLIGHSEGATLSIMSAQKVN
                     ELAGLILLAGPGYKQETLLRNQLKRQNKMLYENDKIDSEDILVKALEDLIKAIENNKK
                     FNIDDYNIPDDFKNIYLSLNNQREFSSQWLKTDPANLLSKVNIPTCIIQGSNDQQVSE
                     EDAKRLSKAVDDEIETYKYMEGINHLLFKDKHQVDKEILDTIINFIKQYE"
     gene            2940..3224
                     /locus_tag="VKN64_10755"
     CDS             2940..3224
                     /locus_tag="VKN64_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60755.1"
                     /translation="MKNFMIFLILVLVAATFLAITNPTKDEFISWGVQEMQADSETDF
                     EKILEGVVGEQVLKVKTTRKNYVIFSIYTVDSGEDNVKYIGIIEQFFQLN"
BASE COUNT         1210 a          404 c          635 g         1038 t
ORIGIN      
        1 tggcagctta taaattgagt aaggggagtt cgattaatga ggaagtaatt acctttcact
       61 ttttagaaga tgtactgggc tgggtatcaa ttttaattgt tggtattatc tatatgttta
      121 agaaaatacc aattctagac cctatattat ctattctaat cacttcctat atactatata
      181 atgtatttaa taattttaaa aagatattaa agatcttctt acaggcagtc ccatctgatc
      241 tttcaattga agaaatagag aagttagtca ttgaaaaaac cgaggtgatt actgtacacc
      301 acacccacct ctggagttta gatggagaaa acaatctgtt aagtacccat attgtggtag
      361 aagacgaatt agataacagt gagatcaata atattaaaga aaagattaga aatttactgt
      421 tagagcataa tatcaaccat gtaactattg aagttgatta tagaagtgaa gtttgtgatg
      481 atgaagaatg taatttaaac ggtaattatt aggatatttt tgggggagat acagtgcaaa
      541 aagggagttt aaaggggtat ctatttcgtt tttcgctagt acatgtaatt atctatgtat
      601 ttatagccct actttttaga aattatttaa attataatga acagtttacc tcttctgtct
      661 tatacaataa ttttagaaat attgattcca ctataatgag atttgcacca ctttttcagt
      721 tggggcgggg gcttttttta ggcttgatta tctaccctgt ctataactgt ataattgggg
      781 ttaaatacgg ttggttgagg ttgtttgccc tcttatgggg tttatccctt atagggtctg
      841 tagcggctac tcctggttct atagagggct ttatatatac aaagacccct ataatagaac
      901 atctgttggg tttgccggaa gtaaccttac agaccatgct attttccctg atctttgtaa
      961 gttgggaaaa aaggagattt aataagcggt tacaaagaaa ggaaattatt gaagaaagta
     1021 caaaagagga gtgaagagat gcgatacttt aagaaattgg taggggaaaa tatttatctt
     1081 tctcctatta atttagaaga ttatgaaaaa tatacggagt ggatcaatga tttggatatt
     1141 tctttaaagt tgggaaacgc tcatcaaata tatacactcc agaaagaaca agaaatatta
     1201 gaagatatat cagaaaattc ttttgcaatt atccttaaag aaaatgataa attgatcggc
     1261 aactgtggtt taattgatgt cgaacaggta acgcgtaaag ctgaattagg tatttttata
     1321 ggagaaaaag aatattggaa tatgggttat ggaactgaag cgattacttt attattagat
     1381 tatggcttta atttactgaa tttacataat atcatgctgg aagtttttgc ctttaataaa
     1441 agggctattg aatgttataa aaagatcggc tttaaagaaa ttggcaggcg gagagaagca
     1501 agagaaatcg ccggcaaaaa gtatgatgaa gtttttatgg atatcttagc tacagaattt
     1561 acggagggca aaataagttt ggagtgatta aacgatggta aaatcaaact tacatacagt
     1621 tttaacattt attttattta tcactttgtt tttttcatta aatgtaaagg cagttgatga
     1681 aatgtttatt gggaactggc agggctttat ttccgtgcaa gaacaaaaat taaatatttc
     1741 tgtaacattt gaacaagtgg gtagtgaatt gaagggtaca attgatatac cccaacaaaa
     1801 tttagttgat tatcctttag atatcagaga agttaatgat aataaaatcg tctttgcgat
     1861 gaataatata cccggtgatc cagtatttga aggtacttta aacaataata aaataacagg
     1921 aacttttaag cagagtaata ataaattaca gttcaactta agtagaactc agaacttaga
     1981 aggaaaaaat gatgaaataa atttaaaagg cagtgaacga gagatagcaa taccggttga
     2041 gggtggaaat atatatggta gtttaacata tcccaaggtt catgatatca atgatccaat
     2101 tgctattata gtggccggtt caggtcctac taatagaaat ggtaataccc ccttgatcga
     2161 acaaaagata aacaatttaa aagatatagc tcattatcta gccaataatg gtatcttatc
     2221 tatcaggtgt gataaaagag gtgtaggtaa aagcagttat ctagttgata gtaaaacccc
     2281 taccttcagc cagtatagag aagatatttt acaggtgata gaatatataa aaagcaattt
     2341 aggaagagaa gaggatcaga tttttttaat cgggcatagt gaaggggcta ctctttcaat
     2401 aatgtcagcc caaaaagtga atgagctagc aggtttaatt ctgttagcag gaccgggtta
     2461 caagcaggaa actttactac gcaaccagct taaaaggcaa aataaaatgc tttatgaaaa
     2521 tgataaaata gatagtgaag atatattagt aaaagcatta gaggatttaa tcaaagctat
     2581 cgaaaataat aaaaagttta acattgatga ttataatatt ccggatgatt ttaaaaatat
     2641 ctatctgagt ttaaataacc aaagggaatt ttccagtcaa tggctaaaaa cagaccccgc
     2701 taatttactt tcaaaagtaa acatccccac ctgtattatt caaggttcaa atgaccagca
     2761 ggtgagtgaa gaggatgcaa aaagattatc aaaggctgtt gatgatgaga ttgaaactta
     2821 taaatatatg gaaggaataa accatttatt atttaaagac aaacatcagg ttgataaaga
     2881 aatacttgat acaataataa actttattaa acaatatgaa taaagaaggg gagaatatta
     2941 tgaaaaactt tatgatcttt ttaatattag ttttagttgc cgcaaccttt ttggctatta
     3001 caaatcctac taaagatgag tttatcagct ggggtgtgca ggaaatgcag gcagattctg
     3061 aaactgactt tgaaaaaata ttagaaggag tagtgggaga acaagtatta aaagtcaaaa
     3121 ctacaagaaa aaattatgtg atttttagta tatatactgt cgatagtgga gaagacaatg
     3181 tcaaatatat aggtatcatt gagcagtttt tccagcttaa ttaattctgc ataataatta
     3241 tattggagct tttaaaaact gatgctaaga ggtggtataa atgagta
//
LOCUS       DATGCJ010000241         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_306867, whole genome shotgun sequence.
ACCESSION   DATGCJ010000241 DATGCJ010000000
VERSION     DATGCJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306867"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..1389
                     /locus_tag="VKN64_10760"
     CDS             460..1389
                     /locus_tag="VKN64_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA60756.1"
                     /translation="MKKLTIFILSFILLMGLVSGSVLAQNGDPIVLGEGDWPGFRAKN
                     SIVEFILENIGYEVERTTARDPILHQGLTQGDIDIHIGSWMPQNTDMRKKYKGQVNYV
                     TQNMTEGVYIMAVPQYVWEAGVKSHADLDKYADKFDKKLYVGPAGWASSQKMNKAIDE
                     DIYGLGDWTAVNSSQSAMMAQLGKSIEDKDWICFVGWKPHWMNTQYDIKYLKDPKRLW
                     ESPYSWVDTLTRPGFEEDYPQVYRFLQQFRVDVEDNSQWINEIGFEERDEMEVAEEWV
                     RNNILKVRRWLSLVKTPEGNNAYQTLLENLNMN"
     gene            1606..2157
                     /gene="dps"
                     /locus_tag="VKN64_10765"
     CDS             1606..2157
                     /gene="dps"
                     /locus_tag="VKN64_10765"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF009990.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HMA60757.1"
                     /translation="MAKVARQMVEDAGVDVDKLLDLLIKNASAELTTFYYYTSLRLNL
                     IGLEGEGIKEIAEDARIEDRNHFEALIPRIYELGGKLPNDLKEFHDISACPPAKLPEN
                     PSDAKEILKVLVEAERCAVRGYTEICNMTQGKDHRTYDLALSILHEEIQHESWFSEFL
                     GEGPSGHFSRKGETSPYVSKFLE"
     gene            2511..>3158
                     /locus_tag="VKN64_10770"
     CDS             2511..>3158
                     /locus_tag="VKN64_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HMA60758.1"
                     /translation="MNKLTIFILSFVLLVGLLSGSVLAQSEEPIMFGEGDWPGIRAKN
                     SVVEFILENIGYEVERTTARDPIIHQGLTQGDIDVFIGSWMPQIMDMRNKYKEEINYV
                     TQNMTEGLYTMAVPQYVYEAGVKSHADLQKHADKFEKKMYVGPAGWASSKKMNKAIDE
                     DIYGLGDWTAINSSQSALMAQIDKSINEEEWIVFVGWRPHWMNAEYNLKYLEDPDR"
BASE COUNT         1137 a          419 c          603 g          999 t
ORIGIN      
        1 ataatagcac ataccccttc tctccttaaa aaagagaagg ggttttaaat atatttaata
       61 tttttaagtc atatggacta aagttactag ctaaaattat aatcaaaata aataaatttt
      121 ttaaatttta gatatttgta catttttaat tacccggatg tacatttact gattaccctc
      181 ttgtacattc atctatcgcc ctacctttaa ttaatagttt aaataattaa tgtttttaat
      241 ataaaaccca aataataaga aaagtatata ttttttaaat tcctcttatc cctatgttta
      301 caagccttat aatcttgaaa ttataaaacc aataataata tattaaataa tatttctatg
      361 tatttaacat cccgataatt attttatata atttatattg ggaaggatta gtaaggataa
      421 tatggaattc atatcaatat aaaaataagg gggataaaga tgaaaaaatt aactattttc
      481 attttatctt tcattctgct gatgggactt gtctcaggtt ctgttctagc acaaaatggg
      541 gatccgattg ttttaggaga aggagactgg cccggtttta gagctaaaaa ttctatcgta
      601 gaatttattt tagaaaacat tggttatgag gtagaaagaa ctacagctcg cgaccccata
      661 ttacatcagg gtctaaccca gggagatatt gatattcata tcggctcctg gatgccccaa
      721 aatactgata tgagaaaaaa atataagggg caggtaaatt atgtaactca aaatatgacc
      781 gaaggtgttt acattatggc tgtaccccaa tatgtctggg aggccggtgt aaaaagtcat
      841 gctgatttag ataaatatgc tgataagttt gataagaaac tttatgtagg accggctggt
      901 tgggcttcat cccagaaaat gaataaggca attgatgaag acatatacgg tttaggtgac
      961 tggaccgctg taaacagcag tcagagtgcc atgatggctc aactgggaaa atcaatagag
     1021 gacaaggatt ggatttgttt tgttggctgg aaaccacact ggatgaatac acagtatgat
     1081 ataaaatact taaaagatcc aaaaagatta tgggaaagtc cctattcctg ggtagataca
     1141 ttaacaagac ctggctttga agaagactat cctcaggtat atagattctt acagcagttc
     1201 agggtagacg tagaagataa cagccaatgg attaatgaaa ttggttttga agaaagagat
     1261 gaaatggaag tagctgaaga atgggtaaga aataatatct taaaagtaag aagatggttg
     1321 agtttagtta aaacaccgga aggaaataat gcctatcaaa ctttattaga aaaccttaat
     1381 atgaattaaa atctaaagta ttaaaaataa ttgtgaatta ttaatatagt tttaaaatac
     1441 taaataatgc tcaaaaagcg tgtggtttct ggaaggaaat tacacgctat tattttttta
     1501 gaataaatat taagaaatgt aacttattta cttgtatcaa tgaaaaatta tctttataat
     1561 taatattaat aataatgatt actattaaag gagaggtgat taattttggc gaaagtagca
     1621 cgtcaaatgg ttgaagatgc tggtgttgat gttgataaac tccttgattt attgataaag
     1681 aatgcttctg cggaacttac aactttttac tactatacaa gtttaagatt aaatcttatt
     1741 ggtttagagg gtgaaggtat taaggaaatt gcggaagatg ctagaataga agacaggaat
     1801 cactttgaag ctttaattcc tagaatttat gaattgggtg gtaagttacc aaatgattta
     1861 aaagaattcc acgatatttc agcctgtcca ccggctaaat tgcccgaaaa tccgtccgat
     1921 gccaaagaaa ttctaaaagt attggtggaa gctgaaagat gtgcagtcag gggttatact
     1981 gaaatttgta acatgactca gggtaaggac catagaactt atgatttagc tttatcgatt
     2041 ttgcatgaag aaatccaaca tgaatcctgg ttctctgaat ttttaggtga aggtccttct
     2101 ggtcactttt ccagaaaggg agaaacttct ccatatgtat ctaaattttt ggaatagaaa
     2161 taagataaaa aatgtataaa atatagtttt ttcaaaagcc cttctcataa tatttaagag
     2221 aagggctttt tgctgtgcaa tcagttaagt taaaatgttg ttatgataca gttgtataaa
     2281 gattattgat gtgtatagaa atattgggga aattatttta taccttttaa aatccccgtc
     2341 ttccctataa ttttaaaggt taaaaataat atataattat taataattat ttaattatga
     2401 ataatatcta tataagtttt acaaaccccc tttatttttc tataatttat taacggtaaa
     2461 acgaggaagt aaaaaagtac aacaggaaaa taaaaataag ggggatcagg atgaataaat
     2521 taactatctt tattttatct ttcgttctgc tagttggact tttatcaggc tcagttttag
     2581 cccagtcaga ggaaccgatt atgtttgggg aaggtgactg gccaggcatt agggcaaaaa
     2641 attcagttgt agaatttatt ttagaaaata ttggctatga agtagaaagg actacggcgc
     2701 gtgaccccat aattcatcag ggtttaaccc agggagatat agatgtgttt attggttcct
     2761 ggatgccgca gattatggat atgagaaata aatataagga agaaattaat tatgtaaccc
     2821 aaaacatgac agaagggctt tatacgatgg cagtacccca atatgtttat gaagctgggg
     2881 taaaaagtca tgctgattta cagaaacatg cagataaatt tgagaaaaaa atgtatgtag
     2941 gtccagctgg atgggcttca tccaaaaaaa tgaataaggc gattgatgaa gatatttacg
     3001 gattgggtga ctggacagct attaactcca gtcagagcgc ccttatggcc cagatcgata
     3061 agtccataaa tgaggaagaa tggatcgttt ttgtcggctg gcgtcctcac tggatgaacg
     3121 ctgaatacaa tttaaaatac ttagaagacc cagataga
//
LOCUS       DATGCJ010000242         7727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_573551, whole genome shotgun sequence.
ACCESSION   DATGCJ010000242 DATGCJ010000000
VERSION     DATGCJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573551"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VKN64_10775"
     CDS             <1..978
                     /locus_tag="VKN64_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMA60759.1"
                     /translation="PQPAERIDNSMEIYDNEILIDVEILNIESASFTQIKDEVGEDEE
                     KIKEKMNEIVNKRGKHHNPVTGSGGMLIGKVAEIGEKLKDRDLKVGDRIATLVSLSLT
                     PLNIEKIKKVHKDIDQVEVEAQAALFESGIYAKLPADMKDTLALAVLDVAGAAAQTAK
                     LVRPGDNVLIIGAGGKSGLLCLHEAKKRAGVTGTVIGMGHSDSSCERIKELGAADIII
                     QGDATQPVETLEKLKEKTGGELADIVINNVNISDTEMASVLTTKDQGTIYFFSMATSF
                     TKAALGAEGVGKDVNMIIGNGYTKGHAAISLEILRENKKLREIFERLYT"
     gene            999..1478
                     /locus_tag="VKN64_10780"
     CDS             999..1478
                     /locus_tag="VKN64_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HMA60760.1"
                     /translation="MNIEEKEENYEMLYDSMKALIEDEKDWIANLANVSALLFNHLEK
                     VNWAGFYLMKEGELVLGPFQGKPACIRIEVGSGVCGSAVEDRKTYVVDNVHQFEGHIA
                     CDAASNSEIVVPLFYEDQIIGVLDIDSPVKSNFDDLDKKYLEKMVNHLIEVSEVETY"
     gene            1505..2371
                     /locus_tag="VKN64_10785"
     CDS             1505..2371
                     /locus_tag="VKN64_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HMA60761.1"
                     /translation="MDINKEKLVMQAVQGAISHPMAARTPYRMDREGKGRILPGTGGI
                     SYNVRIGDPAFGWAGDHIEPGVSMKISDKNKTNDNYGLGLLACIGNKARLISGDAEGT
                     YGYVTGTHGGIDHVIVDFEPEVLEKLKIGDEILIRAFGQGLELDKYPEISVFNLDPNL
                     LDKMNITPKDDFIEVGVTAEIPAHLMGSGIGASSVALGDYDITTGDEEEIKNLGIDKL
                     KLGDFVYLKDCDNTYGREFKKGAGSIGVVVHSDCIKMGHGPGVTTVLTARNNIIRPKI
                     DKNANLINYLYD"
     gene            2476..3033
                     /locus_tag="VKN64_10790"
     CDS             2476..3033
                     /locus_tag="VKN64_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HMA60762.1"
                     /translation="MVKNDMTAQNLRSAYGGESQAYQRYMVWGDKAEEDGFPNVGLLF
                     KAIAHAEQVHASNHFEALDNCMGDFLVASGAVFGLGSTAENLEGAKAGEDFEIDQMYP
                     TYNLVAKEQQENEAVRSFHYALEAEKIHSRLFGEAREAVLDGQDYELESVSICEVCGH
                     TVVDGTPDECPICGAPKEEFKVFEK"
     gene            3086..3436
                     /locus_tag="VKN64_10795"
     CDS             3086..3436
                     /locus_tag="VKN64_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HMA60763.1"
                     /translation="MSKYDVKTLNDPNDKTVLEKKHVPFITAPDSVEKGEVFEVTIKM
                     GKEIDHPMEEGHFIEFFDVYAGDMKVANVTFTPECEAEATLKLKLDESVTLTVEEYCN
                     LHGYWKNEKEVEVK"
     gene            3521..4024
                     /locus_tag="VKN64_10800"
     CDS             3521..4024
                     /locus_tag="VKN64_10800"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HMA60764.1"
                     /translation="MEEVPDHLKKGLKIIFIGFNPGIKSSESGHHYAHPTNRFYKLLY
                     EAGLTDRLYEPSEDHKLLKLGYGLTNIVQRPTKSADGIKSEEYEEGRKELLEKLKKYR
                     PEVACFTGIGVYKEFSQKSNVKRGLQPESHVKGVKEFVISSPSGLNRTPYKKQLAMYK
                     KLKELID"
     gene            4098..5528
                     /locus_tag="VKN64_10805"
     CDS             4098..5528
                     /locus_tag="VKN64_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMA60765.1"
                     /translation="MVERYKDPEWQNKMRVKVDVNNMFKEYIGAEHGIKSEHVDSLEE
                     KVVTAHQSIEKQYEEGKLGFMELPEQQEEVIDEIEQFANNNRDKFDNFVILGIGGSAL
                     GSIALQTALKSPYHNLKEEDGLNIYVPDNVDPERFKSLLDTLDLEKTVFNVISKSGST
                     AETMSLFLIARRTVEEAVGQDNISEHFIATTSKDSGYLRVIAQREGFKMFYIPDNVGG
                     RFSVLTPVGLVYAAFAGIDIRELLAGAAYMKRICSTPDVWENPAYLSGLLQYLAYQQG
                     KVMSVMMPYAHSLKDVADWYRQLWAESLGKEKDREGETVNVGPTPIKALGATDQHSQA
                     QLYMEGPYDKVVNFIEVKEFHQDIEIPDLYEDIKGVSYLRGKSLAELLKTEKKATELA
                     LTRRQRLNNTVIVPKVNEFTMGQMFYLFELETAFVGELFNINAYNQPGVELGKHYTYG
                     VFGREGYEDKKEEYYDRPKKKDKYIV"
     gene            5549..6913
                     /locus_tag="VKN64_10810"
     CDS             5549..6913
                     /locus_tag="VKN64_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMA60766.1"
                     /translation="MEKKYIKYEKDNKEYAAESIFVSKKPRSRVMLSIVKDVKPEINK
                     HIFKNEDIIDYQSVEKIEGDYYLISHEDSCYPPLIDRIKEDDINLKEAVKWTKQIIEI
                     WDSVEQANSFSHEIKANYFRLTEQDNIKLINPFINKKIEQYKYPDLDNEFDEIYRPPE
                     IINREEWDEKARIYNFGVVFYLLTTGKFPFQGEDKQEMYDKKMTGSLIAPRFVNPEIS
                     ETLSNLIVDMLAQNKQNRPDSLTRILEKIDELIKEDSYQASREERENNLNKSGSQHTK
                     NRLKEQVTFYFRHHWGKTLFFGILIGLIIGVGMLGGNPPVITENTTADEVVEFFYSSI
                     DEKDPVVIGQTTTTDLGDLETMVSEGHVMETMRTTYGNLPAEEKEGTNKIFGIKNLNI
                     SKTQKDDYYIYKTNYIFFYPREEEMREKEMQDTLTVKKVENKWQITEIEGSIIPLIEG
                     KFEG"
     gene            6914..7669
                     /gene="nadE"
                     /locus_tag="VKN64_10815"
     CDS             6914..7669
                     /gene="nadE"
                     /locus_tag="VKN64_10815"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HMA60767.1"
                     /translation="MIKLKLDYEHVEKSLIEWLKGKVKDAQAEGVVLGLSGGIDSTVS
                     GILAKKAFPNNTLGLVMPCYSNAQDKVDAIKVAEKFNIDYEVKNLNNIFDSFKDLVGA
                     VEQESQNNLALANAKPRLRMVTLYYYAAQNNYLVLGTDNWSELKVGYFTKYGDGGIDL
                     TILGRLVKTEVRELAEHLDVPREIIDKKPSAGLWQDQTDEDEMGLTYEELDKYILTGE
                     AEPEVKEKIENLSSKNRHKLEPIPVPTREILLT"
BASE COUNT         2725 a         1160 c         1735 g         2107 t
ORIGIN      
        1 ccccaaccgg ctgaaagaat agataactca atggaaatat atgataatga aattttaatc
       61 gatgtagaga tattaaatat agagtcagcc agtttcaccc agattaaaga tgaagtcgga
      121 gaagatgaag aaaaaataaa ggaaaagatg aatgaaatag tgaataaaag aggtaagcac
      181 cataatcccg tgactggttc cggcgggatg ttaattggta aagtagctga gattggagaa
      241 aagttaaagg atagagatct taaagtggga gatagaatcg ctactttagt ttcactttct
      301 ttaactcccc tgaatataga aaaaattaag aaggtccata aagatatcga ccaggttgaa
      361 gtagaggccc aggcagctct ttttgaaagc ggtatttatg ctaaactacc ggctgatatg
      421 aaagacaccc tggctttagc ggtattagat gtggctggag cagctgctca gaccgctaag
      481 ttagtaagac cgggtgataa tgttttaatt attggagccg gtgggaagtc cggcttactc
      541 tgcttacatg aagctaaaaa aagagcggga gtaacaggga ccgtgatcgg aatgggccac
      601 agtgattcca gctgtgaaag aattaaagaa ctaggggcag ctgacatcat tatccagggt
      661 gatgcgaccc aacctgtgga gacactggaa aaattaaaag aaaaaaccgg gggagaactt
      721 gccgatatcg ttataaataa tgtaaatata tctgatactg aaatggcttc tgtgttaacg
      781 acaaaagatc agggtacaat atactttttc tccatggcta ccagttttac aaaagctgct
      841 ttaggagcag aaggtgtagg aaaagatgtt aatatgatta ttggtaatgg ttatacaaaa
      901 ggacatgcag caatttctct tgaaatatta agagaaaata agaagctgag agaaattttt
      961 gaaagactat atacttaagg tggaggagtt ttttgcctat gaatattgaa gagaaagaag
     1021 aaaactatga aatgctttat gattctatga aagccttaat tgaagatgaa aaagactgga
     1081 tagcaaatct ggccaatgtt tcagctttac tttttaacca tttagaaaag gtaaactggg
     1141 ccggctttta tctcatgaaa gagggggaat tagttttagg tccttttcag ggcaaaccag
     1201 cttgtatcag aatagaagtt ggttcagggg tctgtggctc ggccgtggaa gatcgtaaga
     1261 cttatgtagt ggataatgta catcagtttg aaggccatat tgcctgtgat gcagcttcca
     1321 attctgagat tgtcgtacct ctcttttatg aagatcagat tatcggtgta ttagatatag
     1381 acagtccggt aaaaagtaac tttgatgatc ttgataaaaa gtatttagaa aagatggtta
     1441 accatcttat tgaagtttcc gaggttgaaa cttattaatt tgagaaaaaa agaggtgaat
     1501 aatattggat attaacaaag agaaactggt tatgcaggca gtacaggggg caatttcaca
     1561 tccgatggca gcccgtactc cttaccgcat ggatagagaa gggaaaggaa ggatcttacc
     1621 tggaaccgga ggaatctcat ataatgtgag aattggtgat cctgcctttg gttgggccgg
     1681 agatcatatc gaacccggtg tcagcatgaa gatcagtgat aaaaataaga caaatgataa
     1741 ctatggatta ggacttttag cctgtatcgg taataaagcc agacttatca gtggagatgc
     1801 tgaaggcact tatggttacg tgaccggtac ccacggcggg attgaccatg ttattgttga
     1861 ttttgaacca gaagtattag aaaagttgaa aatcggagat gagatattaa ttagagcctt
     1921 tggacagggt ttagaattag ataaatatcc tgagatcagt gtctttaatc tcgatcccaa
     1981 cttacttgat aagatgaaca ttaccccaaa agatgatttt attgaagtgg gagtaacagc
     2041 tgaaatacct gcccacctga tgggatccgg tatcggtgct tctagtgtag cattgggaga
     2101 ctatgatatc actaccggtg atgaggaaga gattaaaaat ctgggtatcg ataaattaaa
     2161 attaggtgac tttgtctact taaaagattg tgataacacc tatggtagag agttcaaaaa
     2221 aggcgccggc agtatcggtg tggtagtcca cagtgactgt attaagatgg ggcacggccc
     2281 gggtgttaca accgttttaa cggcaaggaa taatattatt aggcctaaaa tagataaaaa
     2341 tgctaacctc attaactatt tatatgatta atgaaaataa tagtaaattg acaataatac
     2401 tcatgaatgc tataatcaat tctaacagta ataattattg ataaacttta atcacttaaa
     2461 gggggaatat ataaaatggt aaaaaatgat atgacagcac aaaatttaag atctgcctat
     2521 ggcggagaaa gccaagccta tcagagatac atggtatggg gtgacaaggc agaagaagat
     2581 ggatttccaa atgttggttt gctttttaaa gcgattgctc atgctgaaca ggttcatgcc
     2641 agtaatcact ttgaagcttt agataactgt atgggagatt tcttagttgc ttccggcgca
     2701 gtatttggac ttggttcaac tgcagaaaac ttagaaggag ctaaagccgg tgaagacttt
     2761 gaaatcgatc aaatgtatcc tacatacaat ctggtagcca aagagcagca ggaaaatgag
     2821 gcagttcgtt cttttcacta tgccttagaa gcagaaaaga ttcactccag attatttgga
     2881 gaagcccgtg aagctgtatt agatggtcag gattatgaat tggaatcagt tagtatctgt
     2941 gaagtatgcg gccacacagt agtggacggt actccggatg aatgtccaat ttgtggtgcc
     3001 cctaaagaag aatttaaagt atttgaaaaa taattgctaa taacatataa ttaaagcaga
     3061 ataaaaaaat aaggaggagt ttttaatgtc caaatatgat gttaagacct taaatgaccc
     3121 taatgataag actgttttag agaagaaaca tgttcctttc attactgctc cagatagcgt
     3181 tgaaaagggt gaggtttttg aagtaactat taaaatgggt aaagagatag accatcccat
     3241 ggaagaggga catttcattg agttcttcga tgtttatgct ggcgacatga aagttgccaa
     3301 tgtgacattt actcctgaat gtgaagcaga agctacactg aaacttaaat tagatgaatc
     3361 tgtaacatta accgtagaag aatactgcaa cctacacggt tattggaaaa acgaaaaaga
     3421 agttgaagtt aaataaacat aaataaaaag ttttcaaagt atatcccctc ttcggaacgt
     3481 acgaaggagg ggatattttt atatgtgagg tgattgtaaa gtggaagaag tacctgatca
     3541 tctaaaaaag ggcttaaaaa ttatctttat aggattcaat cccggtataa aatcatctga
     3601 gagcggtcat cattatgccc atcccactaa tcgcttttat aagctacttt atgaagccgg
     3661 tcttactgac cgtctctatg agccctccga agaccacaaa ctgcttaagt tagggtatgg
     3721 tttaacaaat attgtccagc ggccgaccaa atccgcggat gggattaaaa gtgaagaata
     3781 tgaagagggt agaaaagaac tattagaaaa gctaaaaaaa tatcgcccgg aggtggcctg
     3841 ctttacaggg attggagtat ataaagaatt ctcgcaaaaa agtaatgtga aaagaggcct
     3901 gcagccggaa agtcatgtca aaggtgttaa agaatttgtc atatcttcac ccagtggtct
     3961 aaatagaaca ccttataaaa agcagcttgc gatgtataaa aagttaaaag agctgattga
     4021 ttaaaaagat atacttgaaa aacagatatt aaatggtata ctttaattaa atctgaaatt
     4081 aaaaaggagg ccttcctatg gttgagagat ataaagatcc ggagtggcag aataagatga
     4141 gagtaaaagt agatgtaaac aatatgttta aagaatatat cggtgcagaa catggtatta
     4201 aatcagaaca tgtggatagt ctggaagaaa aggtggtaac tgcccatcaa agtattgaaa
     4261 aacagtatga agagggtaag ttgggattta tggaactacc tgaacaacag gaggaagtaa
     4321 tagatgaaat agagcagttc gccaataata atagagataa atttgacaac ttcgttattt
     4381 tgggaattgg tgggtcggcc ttaggcagta ttgctttaca aactgcctta aagagtcctt
     4441 atcataattt aaaagaagag gatggactta atatatatgt acctgataat gttgaccctg
     4501 aaagattcaa gagccttcta gatactttag atctagaaaa aactgttttt aacgtgattt
     4561 caaagtcggg tagtacggct gaaacaatga gcctgttttt aattgcccgc cggactgtag
     4621 aagaagcggt cggccaagat aatataagcg aacactttat tgcaactacc agtaaggatt
     4681 ccggctattt aagggtgatc gctcaaagag aaggatttaa aatgttttat atccccgata
     4741 atgtaggggg gagattttca gttcttaccc cggtcggttt agtctatgca gcctttgccg
     4801 gtatcgatat cagagaactt ctggccgggg cagcctatat gaaaagaatc tgttcaactc
     4861 ccgatgtttg ggaaaacccc gcctatttaa gcggcctatt acagtatctt gcttaccagc
     4921 agggtaaggt gatgtctgtg atgatgcctt atgcccattc cttaaaagat gtggcagact
     4981 ggtacagaca gctttgggcg gaatcattgg gtaaggaaaa agatagagaa ggagaaacgg
     5041 ttaatgtagg cccgactcct atcaaggccc tgggggcaac tgatcagcac tcacaggcgc
     5101 aactatatat ggaaggccct tatgataaag tggttaactt tatagaggtt aaagaatttc
     5161 atcaagatat tgagatcccg gatttatatg aagatattaa aggagtttca tatttaagag
     5221 gtaaaagctt ggctgagcta ttaaaaacag agaagaaggc aacggaactt gccttaacca
     5281 ggagacagcg tttgaataat acggttatag tgcccaaagt taatgaattt acgatgggac
     5341 agatgtttta cctctttgaa ttagagaccg ctttcgtcgg agaacttttt aatatcaatg
     5401 catataatca accgggggta gaactaggta aacattacac ttacggtgtc tttggccgag
     5461 aaggttatga agataaaaag gaagaatatt acgatagacc aaaaaagaag gataaatata
     5521 ttgtctaaaa ggagtaagag attactttat ggaaaaaaaa tatattaaat acgaaaaaga
     5581 taataaggaa tatgcagctg aatccatatt tgtgagcaaa aaacctcgtt cccgggttat
     5641 gttatcaatt gtaaaagatg taaaacccga aattaataaa cacattttta aaaatgaaga
     5701 tattattgat tatcagtcgg ttgaaaagat agagggggat tactatttaa tcagtcatga
     5761 agatagttgt tatcctcctt taattgaccg gataaaagaa gatgatatta acttaaaaga
     5821 agcggtaaag tggacaaaac agataattga aatctgggat tctgtggaac aggctaattc
     5881 gttcagccat gagatcaagg ctaattactt taggctgacg gaacaggata acattaaact
     5941 tatcaacccc tttattaata agaagatcga acagtataaa tatcctgatt tagataatga
     6001 atttgatgaa atataccgcc cccctgaaat tattaacagg gaagagtggg atgaaaaggc
     6061 ccgtatatat aatttcgggg tagtctttta tctgctgacc accgggaaat tcccttttca
     6121 aggtgaggat aagcaggaaa tgtatgataa gaaaatgact ggttctttaa tcgcaccccg
     6181 atttgtaaat ccagaaatat ctgaaacctt aagtaattta attgtagata tgttagctca
     6241 aaataaacag aaccgccccg attccttaac tcgtatcctg gaaaaaatcg atgagttaat
     6301 aaaagaagat tcctatcagg ccagtcgaga agaaagagaa aataatttga ataaaagcgg
     6361 cagtcaacat accaaaaata gattgaagga acaagtcacc ttctatttca ggcaccattg
     6421 gggcaagacc ctcttttttg gcatattgat tggattgatt ataggggtag gaatgttagg
     6481 gggcaatccg cccgtaataa cagaaaatac tacagcggat gaagttgtag agtttttcta
     6541 tagttcaata gatgaaaagg accccgtagt aattggccag actactacca ctgacctggg
     6601 agatttagaa accatggttt cggaaggcca tgttatggaa actatgcgga ccacctatgg
     6661 taatttaccc gccgaggaaa aagagggtac gaataagatt tttggaataa aaaatttaaa
     6721 tattagtaaa acccaaaaag atgattacta tatttataaa acaaactata ttttcttcta
     6781 tccacgcgag gaagaaatgc gagaaaaaga gatgcaggat accttaacag ttaaaaaggt
     6841 tgaaaataag tggcagatta ctgaaattga aggtagtata ataccgctga ttgaaggaaa
     6901 atttgagggt taggtgataa aattgaaatt agattatgaa catgtcgaaa aatcactgat
     6961 agaatggtta aagggtaaag taaaagatgc ccaggcagag ggggtagttt tgggattaag
     7021 tggaggtata gattcaactg tatctggtat acttgccaaa aaggcctttc ccaataatac
     7081 tttgggcctt gtcatgccct gttatagtaa tgcccaggat aaggttgatg cgattaaggt
     7141 tgctgagaag tttaatattg actatgaagt aaaaaatctt aataacattt tcgattcttt
     7201 taaggatttg gtgggggcag tagaacaaga aagtcaaaac aatcttgctt tagcaaatgc
     7261 taaacccaga ctgcgtatgg tgacccttta ttactatgcc gctcaaaata attacctggt
     7321 tttaggtacc gataactgga gcgaacttaa agtaggttac tttacaaaat acggtgacgg
     7381 tggaattgat ttaacaattc ttggtcgttt agtaaaaaca gaggtgagag agttagcaga
     7441 acatctggac gtaccacggg agattatcga taaaaaaccc tcagccggac tctggcaaga
     7501 ccagaccgat gaggatgaaa tgggccttac ctatgaagaa ttagataaat atatcttaac
     7561 tggtgaggca gaacctgaag ttaaggaaaa aatagaaaat ttatcttcta aaaacagaca
     7621 taaacttgaa ccaattccag tcccaaccag agaaattttg ttaacttagc aggagaattt
     7681 taaaaagtat agtattatat tattaagaga acaatttaat attgtta
//
LOCUS       DATGCJ010000243         7013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_130222, whole genome shotgun sequence.
ACCESSION   DATGCJ010000243 DATGCJ010000000
VERSION     DATGCJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130222"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VKN64_10820"
     CDS             complement(<1..1560)
                     /locus_tag="VKN64_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMA60768.1"
                     /translation="MFNFNLRKTLIILFLIVGIVPIAVAGYLFYNNAQGEIENKINKS
                     MAMYGEITDNSLEDFFAEREGEIKVLALNDNIKTSLNKYYSQNLSPEDDDWQYNIHTI
                     DRYLKEFVNQYGYSFAFLTDKNGEVIYSTTEDVPLGTDLSERNYINKSIKGNMNWSEL
                     FYSGVINNNAIVLSTPIRENDFEMIVGTANLTLNQSKIDRIVHNSIDTLGDSADAYLI
                     DSEGLLLTNTLIGQYQNDAALKENIDTRAVELLSGPIGNGNYDFSATEQYREYRDVPV
                     LGELAVTKIGDRPAGLIVEIDQSEVFAGINSLRNYLLIIIALVAVSAALIAYFGANRI
                     FNPLEKFQELFAELAMGDLSVGYPMKSVNCSEIMDCGVEDCPDFGKTGVTCWFDVGSY
                     APEFDNEIYCPKILTGEYDSCEECIVYKKVNENEIKTLGAWFNKLVDKLNNIITDVAD
                     IASDLSSSSQELSASSEEISASAEQVGNAIEEVASGAEEQTAQIEETKGSVDNLSNKI
                     TNVEEKSDDMDQ"
     gene            complement(1905..5702)
                     /locus_tag="VKN64_10825"
     CDS             complement(1905..5702)
                     /locus_tag="VKN64_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMA60769.1"
                     /translation="MIDNNFFESFKEILNKKYIGYILALIILILALTVRMEIIEPLII
                     LFVIFASIQYRRRGAIYSTIFAIITLAIQDLYNLHIQLGSYLIEVFTVIVAAFYIIRS
                     SSQIRDRNFELKERVKELRGLFKISKVIDKPDLKLRNSLEEIVNILPPVWQYSEVTCA
                     RIKYDGKVFKTDNFKETEWCQTADIKINGSKVGEVEVCYLTPQPIEYNDTPFLKEEFE
                     LLKDISERISNVIRNVKQEEQIKENNKFLSITLNSIGDAVIVTDEDGQVVRMNSIAEE
                     LTGWNFAEAEDQPVNKVFKIVNAKTRKEVKNPVKKVFEQGKIMGLANHTKLISKDGTE
                     YHIADSAAPIRDQDDNIYGAVMVFRDFTEKYNLNQEIKRRENLFSTAIKTAPYPMMIH
                     SEEGEVKQINDAWTEISGYDLTDIKHIDDWFEQAYGERRNQVSSYVEQLFEKNKKRHD
                     GEFEIQTKEGEVRIWDFSSAPLGRDEAGNRLVLSIAVDITERKEMINKINKLNRLYNI
                     LSDVNQVIVRNNNLEELFSKTCEVITENGNYKSAWIGKINQNKNKLNITGSAGVHSWL
                     IDDVDIDFDNYDSVSQIENTYDIKADTLVSYINSDNDKINKELKFYNKDRSMAIFPLT
                     VFDNLWGILSLCIDEPNYFDKKEIELLRELTSDLSYAVESIKNEKLRKKSEKELKESE
                     KKYRELFENAPVGVFKSNLKGDLLLANPELLNILGFSTLEGALNYYGNLSEVYVNSDK
                     REELFRKLRNYYQVDNFIFKANKGDGSYIWLKMEARVNKMNEEGSMEIDGFIEDITEK
                     YEMERELAKSEEKYRSLFENQYAVMMIIDPADGQIIDANPAATDYYGWSYQELTSMKI
                     KEVNILPDEEIEMEMETANDGQSHTFEFQHKLADGEIRDVEVYSGPIKIDNKDYLFSI
                     IHDITERKKAEERIKYMTFHDKLTGLYNRAYLEEEMRRIDTKRQLPISLIMGDLNNLK
                     LVNDTYGHERGDQLLIKAAEVIKKSCRKEDIIARWGGDEYVVLLPNTSIETSEKISKR
                     IQKKTKQQEDGELLVSISLGNASKEKPGQDLFKVLNKAENRMYKNKITNRKSARSNVL
                     SAFLNTLREKSYETQEHASRMKELSLKLGNEVGLSSPDMDRLSLLTSLHDIGKVTIPQ
                     EILNKPGKLTNDEWDKIKEHTEAGYRIVSSMDEFADIAEYILYHHERWDGTGYPEGIA
                     GRNIPLLSRILSIVDAFDVMVSGRPYKEAVSEEKALKEIKECSGSQFDPDLVDLFLKL
                     MKE"
     gene            complement(5933..6316)
                     /locus_tag="VKN64_10830"
     CDS             complement(5933..6316)
                     /locus_tag="VKN64_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60770.1"
                     /translation="MRKFLTIYIIITITIFAFSGLISAQIYKGIEFHVDPEYNGPEKN
                     TAGGTASINYNPKDSVIIKAKLWGLKPEIQYFLSTNYEACVFETNKSGAANIQWKNEY
                     MLITYIEIISKYPDFDTILETGPIN"
     gene            complement(6561..>7013)
                     /locus_tag="VKN64_10835"
     CDS             complement(6561..>7013)
                     /locus_tag="VKN64_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMA60771.1"
                     /translation="DEAKRNNDSFEDFAKQFYNHFRHERTLLVSMLDLLVRIAVADNQ
                     FHAQERSYIKRTKEIFNISDSQYESILARYEQSDSGSMEKYYKILEVSPDAKMSEIKK
                     QYRQKVKDFHPDNVIGKGLPEEYKEFAEQKFKEIQDAFEKIKQHRRSS"
BASE COUNT         2017 a         1350 c          950 g         2696 t
ORIGIN      
        1 ttggtccatg tcatctgatt tttcttctac attagtaatt ttattggata agttatctac
       61 actaccctta gtttcttcaa tctgggcggt ctgttcttca gcgccggaag ctacttcttc
      121 aattgcatta cccacctgtt cagctgaagc agatatttct tcactagaag cagatagttc
      181 ttgactggaa gaggagagat cactcgcaat atctgctaca tcagtaatta tgttatttaa
      241 cttatcaacc agtttgttaa accaagctcc taaagttttt atttcgtttt catttacttt
      301 cttatatact atacattctt cacaagaatc atattcccca gttaaaatct taggacagta
      361 aatttcatta tcaaactcgg gggcataact ccctacatca aaccagcatg ttactcccgt
      421 tttaccgaag tcaggacagt cttcaacccc acaatccata atttctgaac aatttacact
      481 tttcatagga taaccaaccg atagatctcc catggctaat tctgcaaaaa gttcctggaa
      541 tttttctaaa ggattaaata ttcgattagc tccaaagtaa gctattagag ctgctgatac
      601 tgctaccaat gctattatta ttaaaagata gtttcttaaa ctgtttatcc cggcaaacac
      661 ttctgactgg tcaatctcaa caattaggcc agctggtcta tcaccaattt tggtaactgc
      721 taattctccc aatacaggta catctctata ttctctgtac tgttctgttg cactaaagtc
      781 ataattacca tttcctattg ggcctgatag taactctact gctcttgtat caatattttc
      841 ttttagggct gcatcgtttt gatactgacc tatcaaagtg ttagttaaaa gtaggccttc
      901 actatctatc aggtaagcgt cagcactatc ccccagggta tcgatactat tatgtactat
      961 cctgtcaatt tttgactgat ttaatgtcaa gttagctgta ccgactatca tttcaaagtc
     1021 attttctcta attggagtag ataaaacaat tgcattgtta tttattacgc cggaataaaa
     1081 tagttctgac cagttcatgt tcccttttat acttttattt atgtaatttc tttctgataa
     1141 atcggtcccc aatggtacat cctctgtagt actatagatt acttcaccat ttttatcagt
     1201 taaaaatgca aaagaataac catattggtt tacaaactct tttaaatatc tatcaatagt
     1261 atggatatta tactgccaat catcatcttc aggagataaa ttttgactgt aatatttgtt
     1321 tagacttgtc tttatattat catttaaagc caatacttta atttctcctt ctctctccgc
     1381 aaaaaaatct tctaaactat tatcggtgat ttccccatac atcgccatcg acttatttat
     1441 cttgttttca atttctccct gtgcattgtt gtaaaataga taacctgcaa ctgctatcgg
     1501 tacaatacct acaatgagaa acaaaattat tagagttttt cttagattga aattaaacat
     1561 tgttagctcc tctctttttt gtaaatttta ttattatgta ttttaaaaat attttttatg
     1621 aaaagcttct ttattatttt tttcacatat aatttaaaaa tttaattact ttcctttcaa
     1681 ttatatacac gaaaatatat tattaataat taaagccgat atttcctatt taacattaat
     1741 tatattctat atactccata attctttata tatctataac atttggacaa ttatacagtt
     1801 tacttaaaca aaaatcgtaa gtttttaaca taatattttt tcaggcaaaa aaaataagcc
     1861 cttatagcaa gggctttttg aaaaatatat ttatttttat atttttattc tttcattaat
     1921 ttcaaaaata aatctactaa atcgggatca aactgcgaac ctgaacattc ttttatttct
     1981 tttaaggcct tttcttcgct tacagcttct ttatacggcc taccgcttac cattacgtca
     2041 aaagcatcta ctatagataa tatgcgggat aacaagggta tattacggcc ggcgattcct
     2101 tccggataac cagtaccgtc ccatctttcg tgatggtaca gtatatactc tgctatatct
     2161 gcaaattcat ccatagaaga tacaatccga taacctgctt ccgtgtgttc tttgatctta
     2221 tcccattcat cgttagttaa tttgcccggt ttgttcaata tctcttgtgg tatggttact
     2281 ttacctatat catgcagtga ggtaagtaag gataacctat ccatgtcagg acttgaaagt
     2341 cccacctcat ttcctaattt taaggaaagt tctttcatcc gactggcatg ttcttgggtt
     2401 tcataacttt tttcacgcag ggtgtttaag aaagcagata gcacattgct cctggcgctt
     2461 tttctgttcg ttatcttatt tttgtacatt ctattctcgg ctttatttaa gactttaaaa
     2521 agatcctgtc ccggcttttc cttgcttgca ttaccaaggg aaattgagac taatagctct
     2581 ccatcctctt gttgtttggt tttcttttgg atccttttac ttattttttc actggtttct
     2641 atagaggtgt tgggtaatag tactacatat tcatctccac cccaacgggc aatgatatct
     2701 tcttttctac agcttttttt aattacttca gccgctttga ttaataattg gtcccctctt
     2761 tcatgcccat aagtatcgtt aaccagttta agattattta gatcccccat tattaaactg
     2821 atgggtagct gtcttttggt atcgatacgg cgcatttctt cttctaaata ggcccggtta
     2881 tataaaccgg ttagtttatc atgaaaggtc atgtacttaa ttctctcttc tgcttttttc
     2941 ctttccgtaa tatcatggat gattgaaaat agataatctt tgttgtctat ttttataggt
     3001 ccactgtaga cctcaacatc tcttatctca ccatcagcca gtttatgctg gaactcaaaa
     3061 gtatgactct gaccgtcatt agcagtttcc atttccattt ctatttcttc atccggtaaa
     3121 atattgacct cttttatttt catggaagtt aattcttgat aactccagcc gtaataatca
     3181 gttgcggccg ggttggcatc gattatctga ccatcggccg gatctataat catcattaca
     3241 gcatattggt tttcaaataa ggatctatac ttttcttcgc ttttggctag ttctctttcc
     3301 atttcatact tttcagttat atcttcaata aatccatcaa tttccatgct gccctcttca
     3361 ttcatcttat taacccgggc ctccattttt agccaaatat atgatccgtc acctttattg
     3421 gctttaaata taaagttatc aacctgatag taatttctca gttttctaaa tagttcttcc
     3481 ctcttatctg aattaacata tacttcgctt aaattaccgt aatagttcag cgctccttct
     3541 aaagtagaaa aacctaaaat atttaacaac tcggggttgg caagtaagag gtctcctttt
     3601 aagttggact taaagacccc aaccggagca ttttcaaaca gttctcggta ttttttctcg
     3661 ctttctttta gctctttttc tgattttttc cttagttttt catttttgat tgattctact
     3721 gcataagaaa ggtcacttgt gagttctctt aataattcaa tctctttttt atcaaagtaa
     3781 ttcggttcat cgatacataa agatagaata ccccataaat tatcaaatac tgttaaggga
     3841 aagattgcca ttgaacgatc tttattatag aactttagct ctttattaat tttatcatta
     3901 tcagaattaa tataagaaac taaggtatca gcttttatat cataggtatt ctcaatttgg
     3961 ctaactgaat catagttatc aaaatcaata tctacatcat ctatgagcca gctgtgaaca
     4021 ccggcagaac ctgttatatt taatttattt ttattttgat taatcttacc gatccaggcc
     4081 gacttataat taccgttctc agtaataacc tcacaggttt ttgaaaaaag ttcttctaag
     4141 ttgttattac gaactattac ctgatttaca tcacttaata tattatataa cctatttaat
     4201 ttattgatct tatttatcat ttcttttctt tcagttatat ctacagcaat acttaagact
     4261 aatctatttc ctgcttcgtc tctccctaag ggggcagaac tgaaatccca tattctaacc
     4321 tcgccctctt tggtttggat ttcaaactcg ccgtcatgtc ttttcttgtt tttttcaaaa
     4381 agttgttcca cataactgct tacctggttt cttctttctc cataggcttg ttcgaaccaa
     4441 tcatctatat gtttaatatc tgtaagatca taacctgata tttctgtcca ggcatcatta
     4501 atctgcttaa cttctccctc ttcagaatgg atcatcatcg gatagggagc tgttttgata
     4561 gcagtggaaa acaggttttc tcttcttttt atctcctggt ttaagttata cttttcggta
     4621 aaatccctaa ataccattac agccccatag atattatcat cttggtcgcg aataggggca
     4681 gcactatcgg caatatggta ttctgtaccg tctttagaaa ttaatttagt gtggttggct
     4741 agtcccatta ttttgccttg ttcaaatact ttcttaaccg ggtttttgac ttcttttcta
     4801 gttttcgcat tgactatctt aaatacctta ttcactggtt ggtcttcagc ttcggcaaag
     4861 ttccagccag ttaattcctc ggcaattgaa ttcattctta ctacttgacc gtcttcatct
     4921 gttactataa ccgcatctcc gattgaattt aaggttatcg atagaaattt gttgttctct
     4981 ttaatctgtt cttcctgttt tacatttcta attacattac taattctttc cgatatatct
     5041 tttaatagtt caaactcttc ttttaagaat ggtgtatcgt tgtattcaat gggttgtggg
     5101 gttaaataac atacttcgac ctctcccacc ttactgccat tgatctttat atcagccgtt
     5161 tgacaccatt cggtctcctt aaagttatca gttttaaaca ccttaccatc atattttatt
     5221 ctggcacagg taacttccga atactgccag actggtggta aaatatttac tatttcttcc
     5281 agagagtttc ttaacttcaa atctggttta tctatgacct tagagatctt aaatagtcct
     5341 cttagttctt tgactctttc ctttaattca aagtttctat ctctaatttg cgaggaactt
     5401 cttatgatat agaaagcagc tacaattaca gtaaaaacct ctattagata gctgcctaac
     5461 tggatatgga gattatataa gtcctgaatg gcaagggtta ttattgcaaa aattgtagaa
     5521 taaattgctc cccgccttct atattgaata ctggcaaata ttacgaataa tattatcaaa
     5581 ggttcaatta tttccattct tacggttaag gctaaaatta agattataag ggccaggata
     5641 tatccgatat actttttatt taaaatctct ttaaaactct caaaaaaatt attatctatc
     5701 attgatgcac cgtcctctaa gaaaataaaa tttattataa tcattcattt atattatata
     5761 tattttataa aggtaaggta tttcctgttt tataagggaa ataatattcc atattacgaa
     5821 atttcataaa aaagacgtgt gatctatttt aaaccacacg cgattagagc ttattattaa
     5881 tttgcttaca ctaccacgta gaacctttga ataattattg gtgaaatgat atttaattta
     5941 tagggccagt ctctaaaata gtatcaaaat caggatattt acttattatc tctatataag
     6001 taataagcat atactcattt ttccattgaa tattagcagc accagattta tttgtttcaa
     6061 agacacaagc ttcataatta gtacttaaaa aatattgaat ttcaggtttc agtccccata
     6121 attttgcttt aataattact gagtccttag gattgtaatt tatcgatgca gttccaccgg
     6181 ctgtattttt ttccggaccg ttatattcag gatctacatg gaattcaatt cctttgtaaa
     6241 tttgtgctga aattaatcct gaaaaagcaa agatggttat ggtaatgata atataaatag
     6301 tcaaaaattt cctcatcctt tttccctcct tatcacaaat ttgagcaata aaaagctggg
     6361 agggaatctt atttttaata atctaatttt atttttcaaa actgctttct attttaatta
     6421 tataaaatat tatcaaagtt aatcacattc aaaattctta aattaattaa caacttccat
     6481 atattttacc ttcaatatta aaatgttttt ggtcaaaaac agaaaacaac ctcctaatat
     6541 ctagaaggtt gttgtttttc ttaggaactt cttctatgtt gttttatctt ctcaaatgca
     6601 tcctgtattt ctttaaactt ctgttctgca aattccttgt actcttccgg tagtccctta
     6661 cctattacat tatctggatg gaagtctttt actttttgcc tgtactgctt tttgatttca
     6721 ctcatcttag catcgggtga aacttccagg attttataat acttctccat agagcctgaa
     6781 tctgattgtt catacctggc taagatactt tcatattggc tgtcgcttat attaaaaatc
     6841 tctttggttc tttttatata acttctttct tgagcatgaa actgattatc agcaacagct
     6901 attctaacta agaggtctag catactaaca agcaaggtcc tttcatgtct aaaatggttg
     6961 tagaactgtt tggcaaagtc ttcaaaacta tcattatttc ttttagcttc atc
//
LOCUS       DATGCJ010000244        10100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_172537, whole genome shotgun sequence.
ACCESSION   DATGCJ010000244 DATGCJ010000000
VERSION     DATGCJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172537"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="VKN64_10840"
     CDS             <1..2265
                     /locus_tag="VKN64_10840"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HMA60772.1"
                     /translation="KVYTLGPLIHNPQVVEDLRQKGVYLAESIDDIDDGILVIRTHGV
                     EPKVLEKAQEKGLNIIDATCPFVKNAHKYAKKLVETDYQTFIFGDRNHPEVKGIFGHT
                     DNKGIIIESVEELQGIDLNENIGLVAQTTQSYEDYLEIISYLLPKAKELKIFNTICNT
                     TGNRQKAAKELAKKVDVMYVIGGHNSANTKRLAEISANTDTPTFHIETVDEINWENIK
                     NVNKIGITAGASTPDWIIKEVLEIMSEEKKEVEGKEQEELEEQVEEQVEEPTEEEVEQ
                     EAEEIVEEAKEEVEEEEAKEAAEESEEETEEAKETAEESEEEAEETVEPAAEAEEDEE
                     AEESEEPTEETAEVEETAEEAEEESETKEFNYSDNDIADLKKGQKVTGTVVEINDDGV
                     YVDVGYKTDGFIPKEELDHRKVNNPRDIVEEDEEIEVIILTLENEEGNMILSKKKADY
                     EKAWERIIEAYEDDEIIEAEVTKEVKGGLVVDVGVRGFIPASHVAVGYVEDLSDYVGD
                     TLRLKVIEVERDKNNVVLSAKKVIEEERAEKKVETLENLEEGDTVKGTVTKLVDFGAF
                     IDLGGIEGLLHISEMSWGRIEHPSEVLDEGEEVEVKVLGVNKEDERISLGLKQLMPDP
                     WEEFARKHYEGEKIEGKITKLVDFGAFMEVADGIEGLIHISQLSQKHVETPDEVVEAG
                     ETRKAEIINIDPEQRRIGLSLKGLEGKQEKEEKKEKKDKKKPEAEGKDGEGETSSGAT
                     IQELVGDIFDKEEE"
     gene            2406..3746
                     /locus_tag="VKN64_10845"
     CDS             2406..3746
                     /locus_tag="VKN64_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HMA60773.1"
                     /translation="MIEVTNVKENSTADKLGIEAGDKILSVNNKDIKDYIDYNYQVAE
                     FNFKLKVKKKNGEIKNYDVQRTYDEKLGIEFDEIVFDGLNKCKNKCIFCFVDQQPPDL
                     RQTLNIKDDDYRFSFLQGSYITLTNLSDKDFERIVKYNLSPLNISVHTTDPQLREYMM
                     KNPKAKNILNQLDYLSENGINFNTQIVLCPDINDKEKLNESINDLSQYYPHILSLAVV
                     PVGLTKYKNENLRIYDEKEAAQILKQIKNWQKQISDKYGENFLYAADEFYLLADMDIP
                     NYDHYNDFPQLENGVGLTRLFRDYYYDIKDKYIDKIKGGKENKEFYLLTSVLGKKAIK
                     PIIEDINESLTNMSINIEVVENKFFGETVTVTGLLTAQDIESKIESVEGENFILPGIT
                     LNDEDMFIDEVDIKDFKDKFIDKKIYVCNDIKDILEVLGDVKNSSGDSRQTKCR"
     gene            3700..5013
                     /gene="der"
                     /locus_tag="VKN64_10850"
     CDS             3700..5013
                     /gene="der"
                     /locus_tag="VKN64_10850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMA60774.1"
                     /translation="MSKTVVAIVGRPNVGKSTLFNRLAGTRISIVEDEPSITRDRIYA
                     DAEWLDHKFKLIDTGGIETESDEAIKSMVKKQSELAIEEADFILFVVDGRAGLTALDE
                     DVAQILRKSNKEVFVVVNKIEDFTNMQEDVWEFYSLGFEVVVPISAEHGKNIGDLLDH
                     VIDGLDEVEEEETEDVINIAIVGKPNVGKSSLINYLVGHERVIVSEKPGTTRDAIDTT
                     IEFNDVKYNLIDTAGLRRKARVNDDIEYYSNIRAIRAIERSDGVLMMIDAQSGVTEQD
                     KKISGYAHDAGKAMVIAINKWDLINADSDEMAHYRDEVYYNLKFLNYVPVSFISALTG
                     KRVEEVLELLEYSVDQSTKRIKTGLLNEVIQEAVQLREPPTKKGKQLKIYYSSQVDVK
                     PPTFVFFVNNPDLMHFAYQRYLENTIRENFGFIGSPIVIKIKKRG"
     gene            5019..5633
                     /gene="plsY"
                     /locus_tag="VKN64_10855"
     CDS             5019..5633
                     /gene="plsY"
                     /locus_tag="VKN64_10855"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HMA60775.1"
                     /translation="MKLVLVFIFAYLLGATPNGYIFGMKFAGKDIRNYGSGNVGATNV
                     ARVAGYKVGLLVAILDIAKGYVAVLMAQYLLLPEYSMGFVFAAVLLAVIGHNWSIFLS
                     FNGGKGVATSVGIILRLFPYSLLVLFIVWIIMIIITKMVSVGSILGAISLPFSVALIY
                     STDTSYLIFSTIITLLIIFSHRSNIKRLINGNENKMSWPPGKEG"
     gene            5637..6644
                     /locus_tag="VKN64_10860"
     CDS             5637..6644
                     /locus_tag="VKN64_10860"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMA60776.1"
                     /translation="MSSKISVIGGGSWGTALAQVAAANIDEVLIYARDQEVVKTINNQ
                     SNNFKYFPDVKLKANIKATNDLEQIIDFSEYVILSVPTSATRTVMKKIADKLTQEQTI
                     LSTAKGLEIETFKTNSEIISEFFEGDIAVLSGPTHAEEVIKEIPTAIVVAAQDKKVAE
                     NIQKKLMTARFRIYTIEDVKGVELAGAVKNIIAVASGVTDGIGYGDNTKAALITRGLA
                     EIVKFGLYLDTKRETYNGLAGLGDLVVTCNSDHSRNRRFGIQIGQGKTFEEAEQIVKQ
                     VVEGAKTAKAVYKFLQENEEIEMPITEEVYKVLYEDKDPHQALDDLMLREPRNEFYYL
                     P"
     gene            6756..7616
                     /locus_tag="VKN64_10865"
     CDS             6756..7616
                     /locus_tag="VKN64_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HMA60777.1"
                     /translation="MSVKIIADSGCDLPKDLLNEYNITEAPLLVYPEGKEDQAEKIDD
                     PKVIYDGMRDGKVFKTAQVPPSFFEKVFTKIAKDNQPAIYIAFSSKLSGTYQSSVVVK
                     NNIEKEYDNLDLTIIDSKCASLGYGMIVYQAAKMAKEGYSKEEIIDTIKHHKKHMEHI
                     FTVDNLEYLRRGGRISRTAAFVGGLLNIKPILDVEDGELVPLEEKRGRRKVMDRMIEL
                     MKERGTNLHDQMIGISHGDDPEGAKKLRNMIQNEFKPKGYIVNMIGSAIGAHSGPGTL
                     AVYFLNDTDY"
     gene            7725..9038
                     /locus_tag="VKN64_10870"
     CDS             7725..9038
                     /locus_tag="VKN64_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HMA60778.1"
                     /translation="MKRRVLIMGAAGRDFHNFNTFYRNNDDYEVVAFTATQIPDIEGR
                     KYPAELAGENYPEGIPIYPESDIDQIIEEENIDEVVFAYSDVSYEYLMSKGSQVMAAG
                     ADFKMMGKESTMLESDVPVIAVCAVRTGVGKSQTTRRVSDILNAMGKKVVVIRHPMPY
                     GDLVAQSVQRYEKYEDMEKNDCTIEEMEEYEPHIERGNILYAGVDYEKILREAEKEAD
                     VILWDGGNNDMPFYNPDLMITLVDPHRAGHELSYYPGEINLRLADVALINKVSTANPE
                     DVAKVRANIQKVNPAATIVDAASPIFVEEPELIKNKRVLVVEDGPTLTHGNMEYGAGA
                     IAARKFGAKELIDPRPYAVGSIKDTFDKYGHLSDILPAMGYGKKQMKELEDTIKNSDA
                     ESVVIGTPIDLTKIIDIDKPHTRVKYELQEIGKPNLQDLIDQLNL"
     gene            9110..>10100
                     /locus_tag="VKN64_10875"
     CDS             9110..>10100
                     /locus_tag="VKN64_10875"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HMA60779.1"
                     /translation="MLKESLKVLREGKIQNNKYVLYWMQAAQRVQDNPALDFAIKRAN
                     DIEVPLVVFFNLTADFPEANLRHYHFMLEGLREVKKDLKELGLKFILKIGNPVINVKN
                     LSKDAVMVISDRGYLKVERAWKKEIAEKIKCRFVLVNTNTIVPVEIASDKEEYAAYTI
                     RKKINNKVERFIDVVDKEKLKVNSLRLNIKGEVLENIDNLINKLSIDKSVKPSPFFKG
                     GSSEALNRLETFMEKNLQLYEEYGSNPDYDVTSKLSPYLHFGQISPAYIYKRIKWNDI
                     KAEDFLEQLIVRRELSFNYIYYNDNYNQKLTDILPDWAVETLEEHESDKREYIY"
BASE COUNT         3772 a         1379 c         2116 g         2833 t
ORIGIN      
        1 aaagtttata ctttaggtcc tttaatacac aacccgcaag tagttgaaga tttaagacaa
       61 aagggggtat atttagccga aagtattgat gatatcgatg acggtatatt agtcatcaga
      121 actcatggtg tagaacctaa agtattagag aaagcccaag aaaagggctt aaatattata
      181 gatgcgacat gtccctttgt caaaaatgct cataaatatg caaaaaagct ggttgaaaca
      241 gattatcaaa cctttatctt cggagacaga aaccatccgg aagtaaaagg aattttcggt
      301 catactgaca ataaggggat aattattgaa tctgtcgagg agctacaagg aatagatctt
      361 aatgaaaaca tcggactagt tgctcaaact acccaatctt atgaagatta tttggaaatt
      421 ataagctact tattaccaaa agcaaaagaa cttaaaatat ttaatacgat ttgtaatacg
      481 acgggtaatc gacagaaagc tgctaaagaa ttagccaaaa aggttgatgt gatgtatgtt
      541 attggtggtc acaatagtgc caatacaaaa cgtttagcag aaataagtgc taataccgat
      601 acaccaacct ttcacataga gacagttgat gagatcaact gggaaaatat taaaaatgta
      661 aataaaattg gaattaccgc aggggcatcg acccctgact ggataatcaa ggaggtcttg
      721 gaaattatgt ctgaagaaaa aaaagaagtt gaaggaaaag aacaggaaga attagaagag
      781 caggtagaag aacaagtaga agaacctacc gaagaagaag ttgaacaaga agcagaagaa
      841 atcgtagaag aagcaaaaga agaagtagag gaggaagaag ccaaagaagc agcagaagaa
      901 tctgaagaag aaacagagga agccaaagaa acagcagaag aatctgaaga agaagcagag
      961 gaaactgttg aaccagctgc cgaagctgaa gaagatgaag aagcagagga aagtgaagaa
     1021 ccaaccgaag aaactgctga agttgaagaa acagccgagg aagctgaaga agaaagcgaa
     1081 actaaagaat tcaattacag cgataacgat atcgctgatc ttaaaaaagg tcaaaaagtt
     1141 actggtactg tagtagaaat taatgatgat ggagtttatg tagatgtagg atataaaaca
     1201 gacggtttta ttcctaaaga agaacttgac caccgcaaag taaataatcc acgcgatata
     1261 gtagaagaag atgaagaaat tgaagttatc atcttaactt tagaaaatga agaaggcaat
     1321 atgattttat ctaagaaaaa ggctgattat gagaaagcct gggaaagaat catagaagct
     1381 tatgaagatg atgaaataat tgaagccgaa gttactaaag aagttaaagg tggtcttgtt
     1441 gttgatgtag gagttagagg atttattcct gcttctcatg ttgctgttgg ctatgttgaa
     1501 gatctcagcg attatgtagg agatactctt agattgaaag taattgaagt tgaaagagat
     1561 aaaaataatg tagttctttc tgctaaaaaa gttatcgaag aagaaagagc ggaaaagaaa
     1621 gtagaaactc ttgaaaactt agaagaaggc gatacagtaa aaggtacagt aaccaagtta
     1681 gtagactttg gtgcttttat cgatctcggt ggaatcgaag gcttacttca catctctgaa
     1741 atgtcctggg gtagaatcga acatccttct gaagtattag acgaaggaga agaagttgaa
     1801 gttaaagttc tgggagtaaa taaagaagac gaaagaattt ctctcggtct caaacaatta
     1861 atgcccgatc cctgggaaga atttgccaga aagcattatg aaggagaaaa gattgaaggt
     1921 aagattacta agcttgttga cttcggtgct tttatggaag ttgcagatgg tattgaagga
     1981 cttatccata tttctcaatt atcacaaaaa catgttgaaa ccccggatga agttgtagaa
     2041 gctggcgaaa ctcgtaaagc agaaataatc aatatagatc cggaacaaag aagaattggc
     2101 ttaagtctta aaggtctcga aggaaaacaa gaaaaagaag aaaagaaaga gaaaaaagat
     2161 aagaaaaaac cagaagccga aggaaaagat ggagaaggag aaacctcttc tggtgctaca
     2221 attcaggaat tagtaggcga tatcttcgat aaagaagaag aataatgtaa aataaataga
     2281 tgaaattatg ggccttacta ttcagtttaa agaatagtaa ggtctttatt ttttgttaat
     2341 agcatagtat attgctaagg cttcaaagat atgttattat tttatgtagc aggaggaaag
     2401 taaaaatgat tgaagtaact aatgtaaaag aaaatagtac agcagataaa ctaggtattg
     2461 aagccggtga taaaatatta tctgttaata ataaggatat aaaggactat atagattata
     2521 actatcaggt tgccgaattt aactttaaat taaaggttaa aaagaaaaat ggtgaaatta
     2581 aaaactatga tgtccagaga acatatgatg aaaaactcgg gatagagttt gacgaaatag
     2641 tctttgatgg gttgaataaa tgtaaaaata aatgtatatt ctgctttgtt gaccaacaac
     2701 cacctgattt acgccaaact ttaaacatca aggatgatga ttaccggttt tcttttttac
     2761 agggcagtta tattaccctg acaaatttgt ccgataaaga ttttgaacgg attgttaagt
     2821 ataacttgag ccctttaaac atttcggttc atactactga tccccagctg cgtgaatata
     2881 tgatgaaaaa tcccaaagca aaaaatatac taaatcaatt agattacttg agtgaaaacg
     2941 gtattaactt caatacccag attgtacttt gtccggatat taatgataaa gagaaattaa
     3001 atgaatctat taatgattta agccaatatt atccacacat attatcgctg gcagtagtac
     3061 cggtcggatt aacaaaatat aaaaatgaaa atttaaggat atatgatgaa aaagaagctg
     3121 cccagatact taaacagatt aaaaattggc agaaacagat tagtgataaa tatggtgaaa
     3181 actttttata tgcggctgat gagttttatc tattagcaga tatggatatc ccgaattatg
     3241 accattataa tgattttcca caactcgaaa atggtgtcgg gctgaccaga ttatttagag
     3301 attattatta tgatattaag gataaatata tagataaaat aaagggaggc aaagaaaata
     3361 aagagtttta tctactaacc tctgttttag gtaaaaaagc cataaaaccg ataattgaag
     3421 atataaatga aagccttact aatatgagta tcaatataga ggtggtagaa aataaatttt
     3481 ttggtgagac agttaccgtg accggtttat taaccgcaca agatattgaa agtaaaatag
     3541 aatcagttga aggtgagaac tttatcttac caggcataac tttaaatgat gaggatatgt
     3601 ttatagacga agttgatatc aaagatttta aagataaatt tatagataag aaaatatatg
     3661 tatgcaatga tataaaagat attttggagg tgttaggaga tgtcaaaaac agtagtggcg
     3721 atagtaggca gaccaaatgt aggtaaatcc accttattta ataggttggc tggtacccgc
     3781 atttcaatcg tcgaagatga acccagtatt acgagggatc ggatatatgc agatgcagaa
     3841 tggctggacc ataagtttaa attaatcgat accggtggaa ttgaaaccga aagtgatgag
     3901 gccataaaat caatggtaaa aaagcagtcc gaacttgcca ttgaagaggc tgactttata
     3961 ctatttgtag tagatgggag agccggttta actgccttag atgaagatgt tgcccaaatt
     4021 ttaagaaaat ctaacaaaga agtttttgta gtagtaaata aaattgaaga ctttaccaat
     4081 atgcaggaag atgtctggga attttattcc ctcggctttg aagtagtagt tcccatttca
     4141 gctgaacatg gtaaaaatat cggtgatctt ttagaccatg ttattgatgg attagatgaa
     4201 gtagaagagg aagaaactga agatgttatt aatattgcta tagtaggtaa accaaatgtc
     4261 ggtaaatctt ctttaataaa ttatctagtc ggtcatgaaa gggtaatagt gagtgaaaaa
     4321 ccgggtacta ccagagatgc gatcgacaca acaatagaat ttaatgatgt taaatacaat
     4381 ttaatcgata cagccgggct cagacggaaa gcgagagtca atgatgatat tgaatattac
     4441 agtaatatta gggcaattag ggcaattgag agatcagatg gggtcctgat gatgattgac
     4501 gcccagtcag gggtgacaga acaggataaa aagatatcgg gctatgccca tgatgctggc
     4561 aaggcgatgg ttattgctat taataagtgg gacttgatta atgctgattc ggatgaaatg
     4621 gcccactata gagatgaagt atactataat ttgaagtttt taaattatgt accagtcagt
     4681 tttatctcgg ccctgacggg aaaaagagtt gaagaagttt tagaattact tgagtacagt
     4741 gtggaccaga gtactaagcg gattaaaacc ggtttattaa atgaggttat ccaggaagcc
     4801 gtccagcttc gagaaccgcc cactaaaaaa ggaaaacagc tcaagatata ttacagcagc
     4861 caggtagacg taaaaccacc tacttttgta ttttttgtta ataatccaga tttgatgcat
     4921 tttgcatatc agagatatct ggaaaacaca ataagggaga attttggttt tatcggaagt
     4981 ccaattgtaa taaaaatcaa aaaaaggggt taaaaactat gaaattagtt ttagttttta
     5041 tatttgccta tttattgggt gcaacaccga atggttatat ctttggcatg aaatttgccg
     5101 gcaaagatat tagaaactac ggtagcggta atgttggggc aaccaatgtt gctcgggttg
     5161 ccggatataa agttggttta ttagtagcaa ttttagatat tgccaaaggg tatgtggcag
     5221 ttttaatggc ccaatattta ttattacctg aatattccat gggctttgtc tttgcagccg
     5281 tattattagc ggtaatcggc cataattggt caatatttct atcttttaat ggaggaaaag
     5341 gggtagcaac cagtgtaggt attatactcc gtctatttcc ctattcctta ttagtactat
     5401 ttattgtctg gattattatg ataataataa ctaaaatggt ctcggtaggt tcaatattag
     5461 gcgccatttc tctccccttt agtgtagcat taatttatag tactgataca tcatatctca
     5521 tttttagtac aataattaca ctcttaatta tatttagcca ccgttccaat attaagagat
     5581 taataaatgg taatgagaat aagatgagct ggccgccggg aaaggaagga taatcaatgt
     5641 catctaagat aagtgtaatc gggggaggca gttggggaac tgccctggcc caggtagcgg
     5701 ctgccaatat agatgaggta ttaatttacg cccgggatca ggaagtggtc aaaacaatca
     5761 ataatcaatc aaataatttt aagtattttc cggatgtaaa attaaaggca aatataaaag
     5821 caacaaatga tttagagcaa atcattgact tttcagagta tgttatttta tctgttccca
     5881 ctagtgctac acggacggtt atgaaaaaaa tcgcagacaa gcttacacaa gaacaaacta
     5941 ttctatctac agccaagggg ctagaaatag aaacttttaa aaccaattct gagataataa
     6001 gtgagttttt tgagggtgat attgctgttt tatccggccc tacccatgca gaagaggtta
     6061 ttaaagaaat tccaactgcg attgtagtgg ccgctcaaga taagaaagta gcagaaaata
     6121 ttcaaaaaaa gctgatgact gcgagattca ggatttatac tatagaggac gttaaaggag
     6181 ttgaactagc cggggcagtt aaaaatatta tagctgtggc ctccggtgta actgatggaa
     6241 tcggttatgg agataataca aaggccgctt taataaccag aggtttagct gaaatagtaa
     6301 aatttggcct ctatttagat actaaaagag aaacctataa tggtttagca ggtttagggg
     6361 atttggtagt tacctgcaat tctgatcata gtagaaacag aagatttggc atccaaattg
     6421 gtcagggtaa aacgtttgaa gaagcagaac agatagtcaa acaggtggta gaaggggcta
     6481 aaacagcaaa agcagtatat aaattcttac aggaaaatga ggaaattgaa atgccgatta
     6541 cagaagaagt atataaggta ctttatgaag ataaggaccc acatcaggcc ttagatgatt
     6601 tgatgttaag agaaccgcgt aatgaatttt attatttacc ataagtcagg aacttagaaa
     6661 tacaaataaa ggattttatt ttcttatatt gaatttaata tattagaatt aatatataaa
     6721 attataatat tactatacct gaaaggggtt tttttatgtc tgtaaaaatt atagctgatt
     6781 ctggttgtga tttaccaaaa gatttactca atgaatacaa tattacggaa gcaccacttt
     6841 tagtctatcc ggaaggtaaa gaagatcaag ccgaaaaaat tgatgatccc aaagttattt
     6901 acgatggaat gagagatgga aaagtattta aaactgctca agtaccaccc agcttttttg
     6961 aaaaagtatt tacaaaaata gctaaagata atcaaccagc tatttatatt gctttttctt
     7021 ctaaattatc aggcacttat caatcgtctg tagttgtaaa aaataatatt gaaaaagaat
     7081 atgataattt agacctgaca atcattgatt ccaaatgtgc ttcgcttggt tacgggatga
     7141 ttgtctatca ggcggctaag atggccaaag aaggttacag taaagaggaa attattgata
     7201 caataaaaca tcataaaaag catatggaac atatttttac agtagataat cttgaatatt
     7261 taagacgcgg tggtagaata agcagaactg ctgcttttgt aggcggtctt ttaaatatta
     7321 aaccgatctt agatgtggaa gatggtgagt tagtaccctt agaagaaaaa agaggtcgta
     7381 gaaaagtaat ggacagaatg atcgaactaa tgaaagaaag gggtaccaat ctccatgatc
     7441 agatgatagg tatcagccat ggggatgatc cggaaggcgc caagaagtta agaaacatga
     7501 ttcaaaatga atttaaacca aagggttata ttgtcaatat gatcggttct gccatcggag
     7561 cccattctgg accaggtacc cttgcggtct attttcttaa tgatacagat tattaaaaat
     7621 ttttaatagt tgtaaggagg atttcagtaa tcaatatata atatatatac taagtcgaat
     7681 aaatataaat aaatgcataa acttattaac aaggaggaaa tttaatgaaa agaagagttt
     7741 taataatggg ggcagccggg agagactttc ataatttcaa tactttttat cgcaataacg
     7801 atgattacga agtagttgcc tttacagcta ctcaaatacc cgatattgaa ggccgtaagt
     7861 atcctgccga attagcaggt gagaactatc ctgaaggtat tccaatttat cctgaaagtg
     7921 atatcgacca aattattgaa gaagaaaata ttgatgaagt tgtttttgct tatagtgatg
     7981 ttagctatga atacttgatg agtaaaggtt cccaggtaat ggcagctggt gctgacttta
     8041 aaatgatggg taaagaatcc accatgttgg aatcagatgt tccagttatc gccgtttgtg
     8101 cggtaagaac tggtgttggt aaaagtcaaa ccacaagaag agtatctgat atactgaatg
     8161 caatgggtaa aaaagttgtt gtaattagac accccatgcc ttatggagac ttagttgcac
     8221 aatctgttca gcgttatgaa aagtatgaag atatggaaaa aaatgattgt accattgaag
     8281 aaatggaaga atatgaacct cacattgaaa gaggtaatat cttatacgct ggtgtagatt
     8341 atgaaaagat attaagagaa gcagaaaaag aagcagatgt aatcttatgg gatggcggca
     8401 ataatgatat gccattttat aatccggatc ttatgattac attggttgat cctcatagag
     8461 caggtcatga actttcctat tatccaggtg agatcaactt gagattagca gatgtagcct
     8521 taattaataa ggtatctacc gctaatcctg aagatgttgc aaaagtaaga gctaatattc
     8581 aaaaagtaaa tccggccgct acaattgtcg atgcagcttc tccaattttt gtagaagagc
     8641 cagaattgat caaaaacaag agagtattag ttgttgaaga tggccctact ttaacacatg
     8701 gcaatatgga atacggagca ggcgcaattg cagcccgtaa atttggtgcc aaagaattaa
     8761 ttgatcctcg cccctatgcc gtaggttcaa ttaaagatac tttcgataaa tacggtcatt
     8821 tatctgatat cttacctgct atgggttatg gtaagaaaca gatgaaagaa ctagaagata
     8881 caattaaaaa ttctgatgca gaatcagtag ttatcggtac accgattgat ctaaccaaga
     8941 tcatcgatat cgataagcct catactagag taaaatatga actgcaggaa atcggaaaac
     9001 caaatctaca ggatttaatt gatcagttaa atctataaat tactgaattt gaagccccct
     9061 cttgtagggg gcttttttca actttagtga aaaagcaagg atgataatta tgttaaaaga
     9121 aagtttaaag gtattaagag aaggtaaaat acaaaataat aagtatgtat tatactggat
     9181 gcaggccgcc caaagggttc aggataaccc ggcattggac tttgcgatta aaagggctaa
     9241 tgatatagaa gtaccattag ttgtattttt taacctaact gctgattttc cggaagcaaa
     9301 cctgaggcat tatcatttca tgttagaagg gttacgggaa gtaaaaaaag atttaaagga
     9361 gttaggcctt aaatttatat tgaaaatagg taatccggtt attaatgtaa aaaatttaag
     9421 taaagatgca gtcatggtaa tatcagaccg cggttactta aaagtagaaa gagcctggaa
     9481 aaaggaaata gctgaaaaga ttaaatgccg ctttgtactt gttaatacca atacaattgt
     9541 cccggttgag attgcttctg acaaagagga atatgcagcc tatacaatta gaaagaagat
     9601 caataataaa gtagaaagat tcatagatgt agttgataag gaaaaattaa aagtaaattc
     9661 tcttcgatta aatattaagg gagaagtctt agaaaatatt gataacttaa taaacaaatt
     9721 atcgatagac aaatctgtta aaccgagtcc ttttttcaaa ggtggttcta gcgaggcttt
     9781 aaatagatta gaaacattta tggaaaagaa cctccaatta tatgaagagt acgggagtaa
     9841 tcccgactat gatgtaactt ctaaattaag tccctattta cattttgggc agatctcgcc
     9901 tgcctatatc tataagagaa taaaatggaa tgatattaag gcggaagact tcttagaaca
     9961 gttaatagtt cgaagagaac ttagttttaa ttatatatat tataatgata actataacca
    10021 aaagttaaca gacatcttac cggactgggc ggttgaaaca ttagaagaac atgagagtga
    10081 taaacgagag tatatctaca
//
LOCUS       DATGCJ010000245         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_83848, whole genome shotgun sequence.
ACCESSION   DATGCJ010000245 DATGCJ010000000
VERSION     DATGCJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83848"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VKN64_10880"
     CDS             <1..181
                     /locus_tag="VKN64_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60780.1"
                     /translation="VFGPWSAAAAAPITHAFICDVQTGVEGNILAYFKLPNLRQPAAG
                     ETLTILVDDCTLKMG"
     gene            192..983
                     /locus_tag="VKN64_10885"
     CDS             192..983
                     /locus_tag="VKN64_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HMA60781.1"
                     /translation="MILKWESADGKVIEFTNKNKYILESTSGFGYPPNDLSSVKSPYQ
                     MGNTLIGQVIQPRDMEIEFIVITDKKDQLFQYKREIIKAFNPIKGIGQLTYIDSTNNK
                     YQIKAVPEQSPIFKEDYKTYQNIKLNLHAPDSRWYNPDPINITLNQSTELINSGDTVT
                     PIEITLDGPMSNPVLINTTAGKKIRLDHSIGSGEKIVINTKFGSKSIYLYDSLGRKRK
                     AFSLLFFDSQLFGLKTGSNIIDLRASNMSEESNATVSYKERYLGI"
     gene            1003..1410
                     /locus_tag="VKN64_10890"
     CDS             1003..1410
                     /locus_tag="VKN64_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60782.1"
                     /translation="MAGSLTNAGETLALNTVFRGTDDIYVGLATSTITETDDLATIIE
                     EDDANYIRELITFTAPADDGTGVFKIENDSLVEFPAFSSDADNNITYAFITKEASATS
                     GDIIAFADLGSGKLPGAGDKLFIPAGGLSISLD"
     gene            1427..2674
                     /locus_tag="VKN64_10895"
     CDS             1427..2674
                     /locus_tag="VKN64_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HMA60783.1"
                     /translation="MALGDIIYDINKSYNITDIEIFESSEKVAFCYGYNAVVYDLIND
                     KSTAEYIHGQLTKGIELSNDETKIASFDSDNVKVWDAVTGDLITTFSGHISYETIRKV
                     AFTKDDSKVVSVSNSEIHVWEITSNNISLTFTDQPGGATSLAISTEGTMIASGGGYDD
                     EILIWDISTGNIIHTLTGHSSHINSIEFHPDGDKIISGSSDNTVKIWDISTEEVISEF
                     TQHTGAVNSCVFFPDGEYVISGGEDSKLYIWDSENNDVLYDKTCSNGVDDVACNSKGS
                     MIAAGIGKAEFIELIDTSGLVLKNNNGQLYNTDPDYLNIPVKLGRIMITESSDIYELN
                     FQNNNGVSIKNVNLSASAEKTGTDLTFGSTMDPFEDLQSITFEGPYEPGTSEKFYIRL
                     NTDLEAVKGENNVEIDASAEFAY"
     gene            2708..4159
                     /locus_tag="VKN64_10900"
     CDS             2708..4159
                     /locus_tag="VKN64_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60784.1"
                     /translation="MEITAALNLQSNTSCVIDTIETSLVKYNLVYNIIDLKTTANINV
                     ESRVEVILEADSKPINQLSSLYNLYDSKLEMNINLTSSLKTSLDKVKKPANITTAYYN
                     IYSLEINFEVSIKSRTEIFVKSSTKPIIKTSGNYVLYSSMVTADISIRSNNIINLLSS
                     PKPEAFTTAKYKLYDVRISAEIDIHDSVSIATGRIDRQRRITSARYQLEDEPGYIRIA
                     INSKPQTDLIITYLSNNESIMVLDPELNLLTYLDDIKYFSWIRRWRRADSFKLKMNRY
                     KDNAKYLKVNNYLVKKTGDVMRGGRIRERQIKVNENGRSSEVWEIKGRGFGNIFNSRV
                     ALHNINIEDGFDTLEASAEKVMKYYVDVNAVNPNDNKRKIPNLRIEENKGLGKIIKYK
                     ARFQKLSDILYDISKVTGLGWDIKFDLKNKEFIFQVLYAELKPEIKLSTNTDSVQKIK
                     FAEDHLNSINTAVVAGQGEGSNRMVVKVTEDDL"
BASE COUNT         1605 a          603 c          837 g         1267 t
ORIGIN      
        1 agtattcgga ccctggagcg cagccgcagc agctcctatc acacatgcct tcatctgtga
       61 tgtgcagacg ggagttgaag gtaatatact ggcttatttt aaacttccga acttaagaca
      121 gccggcagcc ggggaaaccc tgactatact ggtagatgac tgcactttaa agatgggata
      181 ggagaataca gatgatatta aaatgggagt ctgcagatgg taaagtaata gaatttacca
      241 ataagaataa atacatctta gaaagtacat caggctttgg ttatccccct aatgatttaa
      301 gttcagtaaa atccccttat caaatgggga acacattgat tgggcaggta attcagccta
      361 gagatatgga aatagaattt atagttatta cagataagaa agatcaatta ttccagtata
      421 aaagagagat catcaaagcg tttaatccga ttaaaggaat tggtcagtta acttatattg
      481 actccactaa taacaaatat caaattaaag cagtcccaga gcagtctccc attttcaaag
      541 aagattacaa aacttatcaa aatataaaat taaacttaca cgctcccgat tcccgttggt
      601 ataacccgga ccctattaat ataacattga accaatctac tgaactaatt aacagcggtg
      661 atacagttac tccgatagaa ataaccttag atggtccaat gagtaaccct gtgcttataa
      721 atacaacagc aggtaaaaag atcaggttag accattcaat cgggagtggg gaaaagatag
      781 ttatcaatac taaatttgga agtaaaagta tctatcttta tgatagtctc ggcagaaaaa
      841 gaaaagcttt ctccctgctc ttttttgatt cacaactttt cggtctgaaa acaggtagta
      901 atattataga tttaagagca tctaatatga gtgaagaaag taatgcaaca gtaagttata
      961 aggaaagata ccttggaata taaataatct aggagtgaga agatggcagg cagtttaaca
     1021 aatgctgggg aaaccctagc tttaaataca gtatttagag gtaccgatga tatttatgta
     1081 ggattagcta cctctacaat tacagaaact gacgatttag cgacgattat agaagaagat
     1141 gacgctaatt acattagaga attaataact tttacagctc cagccgatga cgggacaggg
     1201 gtttttaaaa tagaaaatga tagtttggta gagtttccag cttttagttc agatgccgac
     1261 aataacatta cctatgcttt tattaccaag gaagctagtg caacttcagg ggacataatc
     1321 gcatttgcag atttaggatc agggaagtta ccgggagctg gagataaatt atttatacca
     1381 gccggcggac tgtcaatatc gctggattaa aaggagatgt aattatatgg cgctaggtga
     1441 tattatttat gatattaata aatcttataa cataacagac attgagatat ttgaaagtag
     1501 tgaaaaggtt gccttttgtt atggatataa tgcagtggta tatgatctaa tcaatgataa
     1561 aagtacagca gaatatatac atggtcaatt aactaagggt atagaacttt ccaatgatga
     1621 aactaagata gcatcatttg atagtgataa tgttaaagta tgggatgcag taacaggtga
     1681 tctgattaca acgttttcag gccatatcag ttatgaaact atacgaaaag ttgcctttac
     1741 aaaagatgat agtaaagtag tttctgtttc aaactctgaa attcatgtat gggaaataac
     1801 ctcaaataat atatcattaa catttacaga ccaaccagga ggagcaacca gccttgctat
     1861 ttccacagag ggaacaatga ttgcttcagg tggtggttat gatgatgaaa tattaatttg
     1921 ggatatatca acaggaaaca taatacatac attaaccggt cattcatcac atataaattc
     1981 cattgagttc catccagatg gagataagat aatttcagga agttctgata atactgttaa
     2041 aatatgggat atatctaccg aagaagttat tagtgaattc actcagcata ctggagctgt
     2101 aaattcctgt gtgttttttc cagatggtga atatgttatt tccggtggcg aggattccaa
     2161 attatatata tgggattcag aaaacaatga tgttttatat gacaaaacct gttcaaacgg
     2221 agtagatgat gttgcctgta attctaaagg ctcaatgata gctgcaggta taggtaaagc
     2281 agaatttatt gaattaattg atactagcgg attagtttta aaaaataaca atgggcagct
     2341 atataataca gatccggatt atttaaatat acctgttaaa ttaggcagaa taatgattac
     2401 agaaagctcg gatatatatg aattgaattt tcaaaataat aatggagttt caataaaaaa
     2461 tgtaaaccta tctgcttctg ctgaaaaaac tggcacagat ttaacttttg gaagtactat
     2521 ggatcccttt gaagacttac agtcaattac atttgaaggt ccttatgagc ctggcacatc
     2581 agaaaagttt tatatcaggc ttaatactga ccttgaagca gtaaagggag agaataacgt
     2641 cgaaatagat gcttctgctg aatttgccta ttaataacac taagtatttg aagaggagtg
     2701 agaaagattg gaaataacag cagctttgaa tcttcaatca aatacatcat gtgttataga
     2761 tacaatagaa acttcattag ttaaatataa tttggtatat aatataatag atttaaaaac
     2821 aactgccaat ataaatgtgg aatctagagt cgaagttatt cttgaagctg atagtaaacc
     2881 tataaatcag ttaagtagtt tatacaattt atatgatagt aagctagaaa tgaatattaa
     2941 tcttacaagc agtctaaaga ctagtttaga taaagtaaag aaacctgcaa atattacaac
     3001 tgcatattat aatatttata gtctggaaat taattttgag gtttcaataa aaagtagaac
     3061 agaaatattt gttaaatctt ctacaaagcc aataataaag actagtggga attatgttct
     3121 ttacagcagt atggtaacag cagatataag tataagaagt aacaatataa ttaatttact
     3181 aagctctcct aagcctgaag cttttactac tgctaaatat aaattatatg acgtgagaat
     3241 cagtgccgaa atagatattc atgattctgt aagtatagcg accggtagaa ttgataggca
     3301 aagaaggata acttctgcta gatatcaatt agaagatgag cccggttata tcaggatagc
     3361 tattaactca aaacctcaaa ctgaccttat tattacctac ttatcaaata atgaatcaat
     3421 tatggtttta gatccggaat taaatctgtt aacctacctc gatgatataa aatacttttc
     3481 ctggatcagg agatggagga gagcagatag tttcaagtta aagatgaaca ggtataaaga
     3541 taatgcgaaa tacttgaaag taaacaatta tcttgtaaag aaaaccggtg atgtgatgag
     3601 aggtggcagg attagggaac gacagattaa agttaatgaa aacggcagga gttcagaagt
     3661 ttgggagatc aagggaagag gtttcggtaa tatattcaac agcagagtgg ccctacataa
     3721 tattaacata gaagatgggt ttgatacttt ggaagcttct gctgagaaag ttatgaaata
     3781 ttatgttgat gttaatgcag tcaatcctaa tgacaataaa aggaagatac ccaatttaag
     3841 gattgaagaa aataaaggct taggtaaaat cattaaatac aaagcaaggt ttcagaaatt
     3901 atctgatatt ctctatgata tctcaaaggt tactggacta ggatgggata taaaatttga
     3961 tttaaaaaac aaagaattca tctttcaggt attatatgca gagttaaaac ctgaaattaa
     4021 gttatcaact aatacagatt cagttcagaa aattaaattt gcagaagatc atttgaattc
     4081 tattaataca gcagtagttg ccggtcaagg agaaggatca aatagaatgg ttgttaaagt
     4141 tactgaggat gatctctagt ggcagttaat gaaagtataa aggcaaagta tatcattgaa
     4201 gaagcagttg atttaccggg catagtaatt gattctggga taaatattaa tattaaagaa
     4261 agtactttaa aactaaggac tgacctagct gagagatctg atgatggttc aa
//
LOCUS       DATGCJ010000246        18829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_130509, whole genome shotgun sequence.
ACCESSION   DATGCJ010000246 DATGCJ010000000
VERSION     DATGCJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 18829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18829
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130509"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..2043
                     /locus_tag="VKN64_10905"
     CDS             466..2043
                     /locus_tag="VKN64_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60785.1"
                     /translation="MSVLQPATIIFFVLIVLNPLIARFLGNLLNLNHFYTEVIISLIL
                     IISYIFFNRFRGDKSKKKLLKKLKLIAAGDFRQEIRLAASSKLSNIADIFNNIMTDIR
                     RLFKKSIQTSVEISEDVDDLSQKIEDVYQTTEEINSAVSEVAQGAEEQSLNLENTNQE
                     IEEILNFVNNTFAKTKEVAATSGKAAEIAEAGNNSIGQLIDKSNKNKKIIQQTKDEMN
                     KLQTNFEEMTEIVGSIEKVSNQTALLALNASIEAARAGDAGRGFGVLAREIRDFSMDV
                     DKLVDNINEVMDRVNKQINTTVERMDENVEIINEQDVRTENLGDDIAQLHSIVSKNYQ
                     KIDSLKEDTKKVTEIIDSSQKSFAKILSISQQTAANSQQVTASIENQQTMIADIEDKV
                     KVVDEMSVELRNFFASKAMDETMLNLCEKIKDRKKKDGLNNQILHEIKEESDIDEIYI
                     VSEAGEFIKSTIDSILGANLFEINEWARDFVEKNAEQVITPIHRRVEDNKLYKFAMVK
                     DNDGEIIEVGLSLESLV"
     gene            2117..4153
                     /locus_tag="VKN64_10910"
     CDS             2117..4153
                     /locus_tag="VKN64_10910"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60786.1"
                     /translation="MFKKAKLSTKILLILVIVSVAAVGINVVIGYTRLNKIKNNTLKS
                     TSRELKTQMQDSIKAKEKVWLTNALQIAHNPIIQEAMYNKDRKTCINMLNEYSQTFKD
                     NTTFNNINVHLIDENLNSFVRSWNTAKFGDSLSYSDAYKQVKETKKPLVTPEMSPKGL
                     RLKGLYPVNYQGEFIGIVNFEGGLNSIKRTLIANDIEFLYLLNNEHLDIATGIKGSPQ
                     VANYTLSQKDVNEEYLNHSTNNLKLASAKAGYFNGDDYLTSSVPIENFNGEEMGIYLV
                     GQSKDIVLNRINQTQDMLYIIYLAFIIIFILQIIAIFYYIKRNVSRPIKLLEDKFELV
                     AEGDLSQVISDKITNRSDEIGNLGTSINKMLGNIKNMVNQIAEIAGNLSASSEELSAS
                     SQEMSASAEEIGRSIQEVASGAQEQSAQVEETSQNVEELTNRIDSVEDMSESMDSQAD
                     NVMHNIHKGNTAIDDSINQVQAVKNQSGAVSTKINELGSLSEEIGDIVELINDISAQT
                     NLLALNAAIEAARAGEAGRGFSVVADEIRELAEESSQATENIANLINDIQNRVDETIK
                     QMNQAEDAVDESVGAIKSTENSFGEINNAAQDLKQLIDNISQAADKMAANSSRVENSV
                     EEIAAVSEQTSSNAEEVAASSQQQSASTTEVVNAAENLAQMAENLTNSISKFKL"
     gene            4176..6242
                     /locus_tag="VKN64_10915"
     CDS             4176..6242
                     /locus_tag="VKN64_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60787.1"
                     /translation="MKKLSYKIILPVTIILIIMAIVLVSFSLFMSGNNITKEADQKLE
                     YMAGQYANDFSNDLVRINSKMKAIKAYVNSTINTSQIESSSYMDSYLSHITPTIKEIA
                     DTTDGILGAYIFFNPDSVAGAHDIYFTKDKEGNLTRQQQISADSYNENDSSMQWFYQP
                     YKTGKSIWTDPFLYEAGDLSANMVSHTSAIVIDGKFIATVGIDLMFDDIKNQIDQINL
                     YDSGYAYIMDEETNFLVHPEYDTSKSIKDIGKNNGAKNIRNTQKGTFKLANNDQILNN
                     GFHKLSNGWIMGVAAPNQEVMAGMNQTRNYLIIIALFATIIAIVAMYVLGNKIAKPIT
                     LSAGITKDIAQGDLSSRVPAKYQNREDEVGTLANSINSMAENLNNIINDISDMANKLS
                     SSSQQLSASSEEISASAEEVGSAIEEVASGAEEQSAQIDETSKNVEGLVAQVETVKDK
                     SGDMDNQADNVMDNINEGNEAIDDSIGQVKEVKEQSNAVSNKINELGELSKEIGNIVE
                     LINDISAQTNLLALNAAIEAARAGEAGRGFSVVADEIRELAEESSQATEQIAGLINDI
                     QSGVKETVNQMNKAEDAVDNSVGAIETTEDSFDKIDEAAKNLRALIEEITNSAEQMAD
                     NSNDVSASIEEISAVSQEASSNAEEVAATSEEQSASTQEIVQAAEELAQMAENLTSTV
                     EQFKTK"
     gene            6305..8275
                     /locus_tag="VKN64_10920"
     CDS             6305..8275
                     /locus_tag="VKN64_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60788.1"
                     /translation="MKLFNKFRVKLLIILIGLMAVPLIIYGLISINNTTDIITADTYD
                     ENHEMAEGLAKDVDYILTSSENMIALATGAQSLKNMHVGHMQKIVDKLVDRSEYIANA
                     YVMDQSGMQIYKTTGELGSRSDRGYFQAAIEGKATFSDVIISRSRGVPIVVHAAPIEK
                     NGEVVGVLGASIDLGILSTLAAETKPGKTGYGFIVDQNGKTIAHPDEEVVMENKDVSH
                     IKPVQEVMKGNNGNTLYTNEGEEKLSSYEPVQKTGWGVVVQLTSAEALNAVDNTIWRT
                     VIIIGITILIGLAAAYFLGNYITAPILAVEEHTGKVANGDLTQEVSAKFLQRKDELGS
                     LAESVNEMTKNLREVVYKISDISNNLSSSSQQLSASSEEISASAEEVGSAIEEVASGA
                     EEQSAQIDETSKNVEGLVAQVETVKDKSDDMDNQADNVMDNINEGDEAIDDSIDQVKE
                     VKVQSSAVSNRINELGELSEEIGNIVELINDISAQTNLLALNAAIEAARAGEAGRGFS
                     VIADEIRELAEESSQATEQIAGLINDIQSGVKETINQMNKAEDAVDNSVGAIETTEDS
                     FDKIDKAAKNLRALIEEISQSAQQMAENTNDVSASIEEISAVSQKASSNAEEVAATSE
                     EQSASTQEIVTAAEELSSMAEQLSDTVNNFKI"
     gene            8314..10281
                     /locus_tag="VKN64_10925"
     CDS             8314..10281
                     /locus_tag="VKN64_10925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMA60789.1"
                     /translation="MSDNNEYLEMFFAESEEYIMILNDNILKLENEPDNMDVLNSLFR
                     AAHSLKGMAATMRFNNLTELTHKMENMLDQIRNHKMKVNTEIIDLLFAGIDYIEETVR
                     QIKDDGSDDLDFSDYLAELEKKIAEDSAATVKQEEIEEEVNGDFLTLTKDEIYTIDEN
                     KKELDSVYAVNVKLDSGTQFKQVRASMIFKSAAELGYVFKSTPVVEEVEEADLDENKV
                     KMVIISNQEQENIEDEFAKITDVEEVMLKTINTEITEKDDKPKKKKKNNTSSTFEVSS
                     SVRVDIGKLDKLMNMIGELLINKTRLEALNIDSEGFKDVLPQLDRVTMELHHTVMQIR
                     MEPIGVMFNRFPRMIRDLSKSSEKEIDFIMEGKQTELDRSIIDELGDPLTHLLRNAVD
                     HGIEPPAERVKKGKDETGTVNLRAYQKGSEIMIEVEDDGGGINVDKVVQKALDKDVIT
                     QKEIDEMDRDKQLQLIFEPGLSTNDKVSNVSGRGVGMDVVKKTIEGLDGEIYIRSEEN
                     EGTKFVISLPLTLAIQDALMVKIDGEIFAIPLSAISETLMIKSEEIKQVKGYDVIVLR
                     DKTIPLIDSRASLNIVIEDDSYKEKDEMPVVIVKSGGKEIGLIVGELLYQQEIVIKSL
                     SDYLDNVKNISGATIIGDGEVALILDVRDIA"
     gene            10304..10795
                     /locus_tag="VKN64_10930"
     CDS             10304..10795
                     /locus_tag="VKN64_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMA60790.1"
                     /translation="MANSEKTVVKAENAGTREQYLTFNVGNEIYGLKLKQAKEIIKPP
                     KITNVPNTEEYILGVINLRGQVIPVINLKNKLGLKENFTAENKTKKIVVINIENMLIG
                     LYVDGVNEVIKLSVDEIEGVSDNRSGIREEFIQGVSSFGDSLIIVLAINNLLFDEKEE
                     KGA"
     gene            10801..11295
                     /locus_tag="VKN64_10935"
     CDS             10801..11295
                     /locus_tag="VKN64_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMA60791.1"
                     /translation="MEAYTKMEESAQNNITGQYIKFKTGNQAFGIDVLNSREIVREEE
                     ITCIPDSPDFVRGVIDLREEIVPIVNLQELFNINTLEEEVKEKKIIIIKVDGTLIGLQ
                     VNRVDEIIEINKETIKDAPSITKKYNKEYIKGVANHEDKLFIILDVNRIFSAEEIKKI
                     KDVK"
     gene            11328..11687
                     /locus_tag="VKN64_10940"
     CDS             11328..11687
                     /locus_tag="VKN64_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMA60792.1"
                     /translation="MKKVLIVDDAAFMRLNLKNIIEEMGFKIIAEAENGIEAVDKYKE
                     HNPDLVTMDITMPEMDGISATKKITSLDDNAKVLVCSAMGQQSLVIDAIEAGAKDFIV
                     KPFERNRIKQAMHKIFK"
     gene            11757..12785
                     /locus_tag="VKN64_10945"
     CDS             11757..12785
                     /locus_tag="VKN64_10945"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMA60793.1"
                     /translation="MINVLIVDDSAFMRQIFKKNIEKESDMQAVAVARNGLDALKKIK
                     RYRPDVITLDIEMPKLDGLETLKELMNKDNFDIPVLMVSALDQKSNVIDALDLGAFDF
                     LPKPSNQISMGIEEITEDLIKKIRAAYESNKSRVKTRQSKPVKSKATRKRIDKFPIVA
                     IGSSSGGPNALKEVLTAFPENFPGGLIIVQHMPAGFTKTLAERLDKLSEIKIAEAKEG
                     DKIKRGKALIAPGNYHLKIVDGEVKLNQQDKKWGVRPCVDYMMETTAQYYKARVIGVV
                     LTGMGHDGGIGMRAIKDQGGYCIIQDKKSALVYGMPQSVIDQNAHHEIASLSEIPMKV
                     MEIIERKF"
     gene            12788..13594
                     /locus_tag="VKN64_10950"
     CDS             12788..13594
                     /locus_tag="VKN64_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HMA60794.1"
                     /translation="MSEFQAFKQQASNILNLDLGGYKEKRIERRTKSLMKRYDIDDFK
                     ECSTRLKEDIKFREAYLNHFTINTSEFFRNPKNYVYLEEKILPKLLENNEKINIWSAP
                     CSNGAEPYTIAIILEEMGARTNHYYILGSDLDPEILAAAKKGVYSPSAVKNVPDKILK
                     KYFKEVDGQFKRYQLSSQILNKVNFEQKDLINGRFRKDDWDLIISRNFFIYLTKEYKN
                     LMIDKFTNVLKDNRYFFIGSTEFIFGSEKFNLKKENLSFYKKISNQAQKN"
     gene            13643..14059
                     /locus_tag="VKN64_10955"
     CDS             13643..14059
                     /locus_tag="VKN64_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03826 family flagellar region protein"
                     /protein_id="HMA60795.1"
                     /translation="MTIRNCKKCGKIYQYDGVHKICMECRRKEEEDFEKVKEFLRDNP
                     NSSINTVSQETGVEKKTIIEFIKSDRLIAEDIEIDVTIRCQRCGKEIKHGKYCDKCIS
                     ELKDEIESIKKKKESKKKKTYRSRENMHVKDRFKDN"
     gene            14228..14512
                     /gene="flgM"
                     /locus_tag="VKN64_10960"
     CDS             14228..14512
                     /gene="flgM"
                     /locus_tag="VKN64_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HMA60796.1"
                     /translation="MKINNNDINMNKIMKAYQNMNQNKNKQVEQKKRTSDQVNISKEA
                     KDIKKMQKSLQEKPDIRRKKVEEIKKQVSNGTYKVNPEKIAEKIVSDLEE"
     gene            14523..15011
                     /locus_tag="VKN64_10965"
     CDS             14523..15011
                     /locus_tag="VKN64_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HMA60797.1"
                     /translation="MSEKLLSELLEILKEENNLYKNLLDIAEDKKETLINNEIDTLFS
                     HVENDRDHIDEISNLEDRRLELMEDIKEDFDIEDDLSYLEFVSQLPDEWENRLNPVRE
                     DLLTTLEEFHVINEENKKLIEEAVRFNKFSIDLIVDNLKNNENTYHSQNESSPRLLDT
                     RG"
     gene            15037..16698
                     /gene="flgK"
                     /locus_tag="VKN64_10970"
     CDS             15037..16698
                     /gene="flgK"
                     /locus_tag="VKN64_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HMA60798.1"
                     /translation="MFDGINIGLSALQTQKKSIETTGHNIANANNEDYSRQRVIQTAK
                     EPNILPGSNFMQGTGVKVEDIRRIQDQFINQQVREESQESGYWKQIQSGMERIEYIFN
                     EPSDSGLQSAFDKFWNSLQDLNTYPNDTATRAAVKNQGMVLSKAFRNINEQMMDYKRS
                     LNSDLEKKVSEINSIADRISRYNKQITTALASNNTPNDLLDKREALFNDLNELTTVKG
                     KTDKVGNLNITIDGKKLVSKFDTNKLEVAESDNKPYADQVIIDISGNKVNIKPETGEI
                     RGIMDVRDQEIPNYLDTLDDIAKSFADKFNEKHSNGFDLKGNTGNEFFELNSSYSRPS
                     RAIQVHSAIKADGGINKIAAGNYSENPGVSSVEITEPGNIDKNQYYKVEVSKSGDQYD
                     YELFEKDAGDPTFSSLVTTGSLDIETAAENPVDVSSRGFEFTVKGNGETVINFNENQG
                     NGNNAASLAKTINQDEILNGATVSDNYKALISTVGVNAQRADQMKASQGDLVKQLENI
                     KKSVSGVSLDEEMANMIKYQQAYNAAAKLITKNDEMLDSLMAMIR"
     gene            16709..17890
                     /gene="flgL"
                     /locus_tag="VKN64_10975"
     CDS             16709..17890
                     /gene="flgL"
                     /locus_tag="VKN64_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HMA60799.1"
                     /translation="MRITDGMMVGNMLKNLQNNRSELDKLNQQLSSGKQFSKPSDDPI
                     GVTTSMGFHTKMNNYEQYQKDVNNAKSWLESTENSLSDAGKILQRSSELAIYGANDTL
                     NPDDRKNMSEEVKELRNELISIANSKLGQDYLFAGQASGTKPIEVNNEYHTNNDLTLN
                     NQNNISSVKRSSSTALTPGNYTVSLEENGGTVDTINISNEEGEIVASKTNDTALTQDT
                     EYEVETDNGEKLTIKTGNNNPTVTDQAEFEIHSYVKYKGDHNKILRKISDDNNMKVNA
                     NADKVFKQNIEIVNNLYEDLKAGKGGEKISAYATKLQNGMTNNTGARAEVGAKINRLD
                     LITNRIDDELLNTEKLNSKNEDIDLAKLVTDLKMSENVYRASLSSAARVIQPSLVDFL
                     K"
     gene            17925..18371
                     /gene="fliW"
                     /locus_tag="VKN64_10980"
     CDS             17925..18371
                     /gene="fliW"
                     /locus_tag="VKN64_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HMA60800.1"
                     /translation="MEILTRDFGKVNIDEDKIIEFPDGLPGFKEEKEFVLLPLDEDSL
                     FVIMQSVNTPELAFITIEPRNIIKDYEFVISEKTEELLQIEGIEDIILLNIVNIKDKL
                     QDMTINLAAPLVININKNLGKQIILDDSKYPVKFKLENNEAKEMMS"
     gene            18371..18616
                     /gene="csrA"
                     /locus_tag="VKN64_10985"
     CDS             18371..18616
                     /gene="csrA"
                     /locus_tag="VKN64_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HMA60801.1"
                     /translation="MLILTRKENEKILIDEEIEISIVEIKGNKVKIGINAPEEVEILR
                     EEVYKSVKEENIEATKDRKVNLDKLKGFGKNSKNSKK"
BASE COUNT         7190 a         2828 c         3846 g         4965 t
ORIGIN      
        1 attacaatat ggaataaatt aaagttatag aaactcaata ctgagcataa tagtattagg
       61 gctaatatca taaaaaattg ttgtctgttt ttaagtagtt tattcttcat tttaaacctc
      121 cgtgtttaat aattctatat atatcaaaat ttcccttttt attattatat gaaaccataa
      181 tataaatcaa aattcaaaaa atcacagaat ataataaatt aatattttca ataactgtaa
      241 aaaacgaaat ttattatttc atggtgtata agctaataat agagaatata tgagagttaa
      301 tctgatttaa tagtttgaat ataaatttaa taataatcct ataaatacga ataaaaagat
      361 aagtacagaa aaaaaggata ggtagaacta aactgctgta tttacaacag aatatataaa
      421 tttttctatt attaaaaata ttaagttaaa aaggggagat atttggtgtc ggtattacaa
      481 ccagccacaa tcattttttt cgttttaata gttttaaatc cattgatagc aagattttta
      541 ggaaatctac ttaatttaaa tcacttctat accgaggtaa taatcagcct catattaata
      601 atttcctata ttttctttaa tagatttaga ggcgataaaa gcaagaaaaa gcttttaaaa
      661 aagttaaaac tcattgcagc cggagatttc aggcaagaga ttagactggc tgccagtagt
      721 aagttatcta acattgccga tattttcaat aatatcatga ccgatatcag gcggcttttt
      781 aaaaaatcaa ttcaaacctc agttgaaata tcggaagatg tagatgattt aagccaaaaa
      841 attgaagatg tctaccaaac aaccgaagag attaactccg cggtaagcga agtagctcag
      901 ggagcggaag aacagtcgct caatttagaa aacacaaatc aggaaattga agaaatctta
      961 aatttcgtga acaacacctt tgctaagaca aaagaggtag cagccacttc tggtaaagcg
     1021 gccgagatag ccgaggccgg caataattcg ataggccagc tcattgacaa gtcaaataaa
     1081 aataaaaaaa taatccagca gaccaaagat gaaatgaata aactgcagac caactttgaa
     1141 gagatgaccg aaatagttgg ttcgatcgaa aaggtatcta accagaccgc tttacttgcc
     1201 cttaatgcct caatagaggc agcccgggcg ggcgatgccg gccgtggttt tggtgtttta
     1261 gcccgcgaaa taagggactt ttccatggat gtagataaac tggtcgataa tatcaatgaa
     1321 gtcatggaca gggtaaacaa acagattaac accacggtcg aaagaatgga tgaaaatgtt
     1381 gaaattatca atgaacagga cgtaagaaca gagaatttag gggatgatat tgctcaactt
     1441 cattctatag tttctaaaaa ctatcaaaag atagatagtt taaaagaaga taccaagaaa
     1501 gtaacagaga ttattgatag ctcccagaaa tcatttgcca aaatcctttc tatttctcag
     1561 cagactgctg ccaacagcca gcaagtaacg gcttctattg aaaaccagca gaccatgata
     1621 gctgatatag aggacaaggt gaaagttgtc gatgaaatgt cagttgaact aagaaacttc
     1681 tttgcctcca aggcgatgga tgaaacgatg ttgaacttat gtgagaaaat taaagataga
     1741 aagaaaaaag atggtttaaa caaccaaata ctgcatgaaa taaaagaaga atctgatata
     1801 gacgaaatat atattgttag tgaagcaggc gaattcatta agagtacaat cgacagtatt
     1861 ttaggtgcca acctatttga aatcaatgaa tgggcgagag attttgtgga aaagaatgcc
     1921 gagcaggtaa taactcctat ccaccgccgg gtagaagata ataaattata taaatttgca
     1981 atggtcaaag ataacgatgg agaaataatc gaagtaggat tgagtttaga atcattagtt
     2041 taattaatcg atgaaacaaa taatactctc ataaatacta taaaataagc caatgtgaaa
     2101 ggatgagagg caaaatatgt ttaaaaaagc aaagttaagt acaaaaatat tgttgatctt
     2161 agtaatcgtt tcggttgcag ctgtcggaat aaatgttgtc ataggttata cgagattgaa
     2221 taagattaaa aataatactt taaagagtac cagccgggaa ttaaaaacac agatgcagga
     2281 tagtattaag gcaaaagaaa aggtgtggtt aacaaatgcc ttacagattg cccataatcc
     2341 gattatacag gaggccatgt ataataagga ccgtaaaacc tgtataaaca tgttaaatga
     2401 gtatagtcag acctttaaag ataatactac ttttaataat ataaatgttc atttgatcga
     2461 tgaaaactta aattcctttg taaggtcctg gaatacggcc aagtttggag acagtctaag
     2521 ttactcagac gcctataaac aggtaaaaga gactaaaaaa ccactggtta caccagagat
     2581 gtcacccaag ggtttaaggc taaaaggact ttatcccgtt aactaccagg gggaatttat
     2641 cgggatagtg aactttgaag gcggtttaaa ttcgataaaa aggactttaa ttgcaaacga
     2701 cattgagttt ctgtatctat taaataatga acacttagat atagctaccg gtattaaggg
     2761 aagtccccag gttgctaatt acaccttaag tcaaaaagat gtaaatgaag agtatttaaa
     2821 ccattcaact aacaatttaa agttagccag tgctaaggca ggttacttta atggagatga
     2881 ctacttgact tcttctgtac caattgaaaa ttttaatgga gaagaaatgg gaatttactt
     2941 agtaggccaa tccaaagata ttgtcttaaa taggataaac cagacccagg acatgttata
     3001 tatcatctat ttggctttta tcataatctt cattttacag atcatagcca tcttttatta
     3061 tataaaaaga aatgtcagcc ggccaattaa actgcttgaa gataaatttg aactggtcgc
     3121 agagggagat ctatcacagg tgatttcaga taaaataaca aatcgcagtg atgaaatagg
     3181 taatctcggc acctcaatca ataagatgtt aggtaatatt aaaaacatgg taaaccagat
     3241 tgctgaaatt gcaggtaatc tctccgcttc cagtgaagag ttatctgctt ccagtcaaga
     3301 aatgtcagct tctgccgaag agatcggcag atcaattcaa gaggtggctt ctggagccca
     3361 ggaacagtca gcccaggtag aagaaaccag ccaaaatgta gaagaattaa ctaatcgaat
     3421 agacagtgta gaggacatgt ccgagtccat ggacagccag gctgataatg tcatgcataa
     3481 tatccataaa ggtaatactg ccattgatga ttccatcaat caggtacagg ccgttaaaaa
     3541 tcagtccgga gctgtttcaa ccaagataaa tgaattaggt tctctctcgg aagagatcgg
     3601 agatatagtt gaactgatta atgacatttc agctcagacc aatctgttag ccttaaatgc
     3661 ggccatcgaa gccgcccgag caggtgaagc ggggcgcggt ttcagtgtgg ttgctgatga
     3721 gataagagaa ctggctgaag aatcatcaca ggccactgaa aatattgcga acttgattaa
     3781 tgatattcaa aatagagttg atgaaacaat aaaacagatg aaccaagctg aagatgcggt
     3841 tgatgagagt gttggggcga ttaagtctac agagaactcc tttggagaaa ttaataatgc
     3901 agctcaagat ttaaaacaat tgatcgacaa tatttcgcaa gctgccgaca agatggctgc
     3961 taacagcagt cgggtagaaa actctgtaga agaaattgcg gctgtcagtg agcagacttc
     4021 cagtaatgca gaagaggtag ctgcctccag ccaacagcag agtgcttcta ctactgaagt
     4081 tgttaatgca gctgaaaatc tggcccaaat ggctgaaaat ctaacaaact caatttctaa
     4141 attcaaacta taaaaattat aaggagttga ataatttgaa aaaactaagt tataaaatca
     4201 tcttaccggt aactattata ttaattatca tggcgattgt tttagtttct ttcagtttat
     4261 ttatgtcagg taataatatt acaaaagagg cagaccaaaa gttagaatac atggccggcc
     4321 agtatgctaa tgattttagt aatgatttag taaggatcaa tagcaaaatg aaggccatca
     4381 aggcatatgt aaacagtaca attaatacta gtcagattga aagttcaagt tatatggatt
     4441 cctatttatc acacataaca ccgacaatta aggaaatagc agacacgact gatggtatct
     4501 taggcgcata catatttttt aatcctgata gtgtggccgg tgcccatgat atttacttta
     4561 caaaagataa agagggcaat ttaacccggc agcagcaaat ttcggccgat agttataatg
     4621 agaatgactc atctatgcag tggttttacc aaccctataa gacgggcaaa tcaatatgga
     4681 cagacccctt cttgtatgag gctggtgatc tttcggctaa tatggtatcc cacaccagtg
     4741 ctatagtaat agatggtaaa tttattgcta cagtcggtat tgacctgatg tttgatgata
     4801 ttaaaaatca gatagatcaa attaatcttt atgattcggg ttatgcatat ataatggatg
     4861 aagaaactaa ctttttagta catcccgaat atgatacctc aaaatcaata aaagatatcg
     4921 gaaaaaataa tggagcaaaa aatatccgaa atactcaaaa aggaaccttt aagctcgcaa
     4981 ataatgatca aattttaaat aatggttttc ataaattatc taatgggtgg ataatgggag
     5041 tagcagcacc caatcaagaa gtaatggccg gtatgaacca gacaagaaac tacttaataa
     5101 taatagcttt atttgcaaca attattgcta tagttgccat gtatgtactc ggtaataaaa
     5161 ttgcaaaacc gattacattg tcggcaggga ttacgaaaga tattgcccaa ggagacttaa
     5221 gttcgagagt tccggctaaa tatcaaaata gggaagatga ggtcggtact ttagccaatt
     5281 ccataaatag tatggcagaa aatttaaaca atattataaa tgatatatct gatatggcta
     5341 ataaattatc atcttccagc cagcagttat cggcctcaag tgaagagata tctgcttctg
     5401 ctgaagaggt tggttctgct atcgaagaag ttgcctcagg ggcagaagaa cagtccgccc
     5461 agattgatga aacaagcaaa aacgtggaag gacttgttgc ccaggtcgaa acagttaaag
     5521 ataaatcagg tgatatggat aaccaagctg acaatgtgat ggataacatc aatgaaggta
     5581 atgaagctat agatgactcc attggccagg ttaaagaggt aaaagaacag tccaatgcag
     5641 tttctaacaa gatcaatgaa ctgggagagt tgtccaaaga aatcggcaat attgttgaac
     5701 tgatcaatga catttcagct cagaccaacc tacttgcttt aaatgcagca attgaagctg
     5761 cccgagcggg tgaagcgggc cgcggtttca gcgtagttgc cgatgagatt agagaattgg
     5821 cggaagagtc atcccaggct acagaacaga ttgcgggatt aattaatgat attcaatccg
     5881 gtgtgaaaga aactgttaac cagatgaata aagcagaaga tgccgtggat aacagtgtcg
     5941 gtgctatcga aacaacagag gattcctttg ataaaataga tgaagcagct aaaaacttaa
     6001 gagctttaat agaggagata actaattcag ctgaacagat ggctgataac agcaatgatg
     6061 ttagcgcttc tatcgaagaa atatcggctg tcagccagga agcctcaagt aatgcggaag
     6121 aagttgctgc caccagtgaa gagcagagcg cttctactca ggagatagtc caagccgcag
     6181 aagaactagc acagatggct gagaatctta caagtacagt cgaacagttc aaaacgaaat
     6241 aatttaaaat aataatttac aataatggat gataaaaaat agtactatat ttaggaggtt
     6301 ttacatgaaa ttgtttaata agtttcgagt taaattatta atcattttaa tcggcttaat
     6361 ggcagtaccg ctcataatct atggtttaat ttcaattaat aatacgactg atataataac
     6421 cgcagatact tatgatgaaa accatgaaat ggcggaagga ttagccaaag atgtagacta
     6481 tattctaacc agcagtgaaa atatgattgc tttagcaact ggagcccaga gtttgaaaaa
     6541 tatgcatgtg ggacatatgc agaaaatagt ggataaactg gtagaccgca gcgaatacat
     6601 cgcaaatgct tatgtaatgg atcaaagcgg aatgcagatc tataaaacta cgggagaact
     6661 aggtagtagg tcagatcgcg gatatttcca ggctgcgatc gaaggaaagg ccactttttc
     6721 cgatgtaata atatcaagga gtaggggagt accgattgta gttcatgcag cccctatcga
     6781 aaaaaatggt gaagtagttg gtgtattagg agcaagtata gatttaggta ttttaagtac
     6841 acttgctgct gaaaccaagc ccggcaaaac tggttacggt tttattgtag atcaaaatgg
     6901 taagacaatc gctcatcctg acgaagaagt tgttatggaa aataaagatg tatcccatat
     6961 aaaacctgtt caagaagtaa tgaaaggtaa taatggaaat actctttata ccaatgaagg
     7021 ggaagaaaaa ctttcttcct atgaaccggt tcaaaaaacc ggttggggag tagttgtaca
     7081 actgacttca gctgaagcat taaatgcagt ggataataca atttggagga ccgtaataat
     7141 aatcgggatt acaattttaa tcggtttagc agctgcctac tttttaggta actatattac
     7201 agctcccatt ctggcggtag aagaacatac tggtaaggta gcaaacggag atttaacaca
     7261 agaagtatct gctaaatttt tgcaaagaaa agatgaatta ggcagtttag ctgagtcagt
     7321 aaatgaaatg acaaaaaact tacgagaagt cgtctataag atctctgata tttccaacaa
     7381 cctatcttct tccagtcagc agttatctgc ctccagtgaa gagatatctg cttctgcgga
     7441 agaggttggt tctgccatag aagaggtcgc atcaggagcg gaagaacagt ccgcccagat
     7501 tgatgaaacc agcaaaaacg tggaaggact tgttgctcag gttgaaacag ttaaagataa
     7561 atcagatgat atggataacc aggctgacaa tgtaatggac aatattaatg aaggtgatga
     7621 agctatagat gattcaattg atcaggtcaa agaggtaaaa gttcagtcca gcgcggtctc
     7681 caacaggata aatgaactgg gtgaactctc agaagaaata ggcaatatag tagaattgat
     7741 caatgatatc tcagctcaaa ctaatttact tgccttaaat gcagcaattg aagctgcccg
     7801 agcgggggaa gcgggccgcg gtttcagcgt aattgccgat gagattagag aattggcgga
     7861 agagtcatcc caggctacag aacagattgc gggattaatc aatgatattc agtctggtgt
     7921 gaaagaaact attaaccaga tgaataaagc tgaagatgcc gtggataata gtgttggtgc
     7981 tatcgaaaca acagaggatt cctttgataa aatagataag gcagctaaaa acttaagagc
     8041 cttaatagaa gaaattagtc agtcagctca acagatggct gagaacacaa atgatgttag
     8101 tgcttctatt gaagaaatat cggctgttag tcagaaagct tctagtaatg ccgaagaagt
     8161 tgctgcgacc agtgaagagc agagtgcttc cacccaggag atagtaacag cagcagaaga
     8221 gctttccagc atggcagaac aactatctga tacagtaaat aattttaaaa tataaattaa
     8281 atgtaagaat aaacttttta aggaggaact atagtgagtg acaataatga atatttagag
     8341 atgttttttg cagaatcaga agaatatatt atgatcttaa acgacaatat tttaaaactt
     8401 gaaaatgaac ctgataatat ggatgtgctt aactccctat tcagagctgc ccattctctg
     8461 aaaggaatgg cagctaccat gcggtttaat aacttaactg aattaaccca taagatggaa
     8521 aacatgttag atcagatcag aaaccataaa atgaaggtaa atacagaaat tatcgatctg
     8581 ctatttgctg gaattgacta tattgaagaa accgtccgtc agatcaaaga tgacggtagt
     8641 gatgatttag acttcagtga ctacctggct gaattagaaa agaaaattgc tgaagacagt
     8701 gctgccactg ttaaacaaga agagatcgaa gaggaagtaa atggtgattt tcttaccctt
     8761 acaaaagatg aaatttatac tatcgatgaa aataaaaagg aattagattc agtctatgca
     8821 gttaatgtaa agctagatag cggtactcaa tttaaacagg taagggcttc tatgatcttt
     8881 aaatctgcag ctgaattggg atatgtcttt aaatcaactc ctgttgtaga ggaagtagaa
     8941 gaggcagatt tagatgagaa caaggttaaa atggtcatca tctcaaatca ggagcaggaa
     9001 aatatcgaag atgagttcgc aaaaattacc gatgtagagg aagtcatgct taaaactata
     9061 aatacagaaa ttacagaaaa agatgataaa cctaaaaaga aaaagaaaaa taatacttct
     9121 tctacttttg aagtttcatc ttcagttaga gttgacatcg gtaagttaga taaattaatg
     9181 aatatgatcg gtgagctttt aattaacaaa accagattag aagccctaaa tatagactca
     9241 gaaggattta aagatgtact acctcagtta gatagggtaa ccatggaact acaccatacc
     9301 gtaatgcaga tcagaatgga accgatcggg gtcatgttca accgctttcc gcgcatgata
     9361 agagatctat ctaaaagcag tgaaaaagag atcgacttta tcatggaagg taagcagacc
     9421 gaactcgacc ggtcaattat agatgagctg ggcgatccat taactcacct acttagaaat
     9481 gcagtggacc acggtattga gccaccggct gaaagagtaa aaaaaggcaa ggatgaaaca
     9541 ggtactgtta atttaagggc ctatcaaaaa ggcagtgaaa tcatgataga agtagaagat
     9601 gatggtggtg gaattaatgt cgacaaggtt gttcaaaaag ctttagataa agatgtaata
     9661 acccaaaaag agatagatga aatggataga gacaagcaat tacagctcat ctttgaaccg
     9721 ggcttaagca ccaatgataa ggtttcaaat gtatcaggtc ggggtgtagg catggatgtc
     9781 gttaaaaaga caatagaagg cttagacggt gaaatatata taaggtcgga agaaaatgaa
     9841 ggtactaagt ttgtaatctc tctgccatta accctagcta tccaggatgc cttaatggtt
     9901 aagattgacg gtgaaatatt tgcgataccc ttaagtgcga tcagtgaaac cttaatgatt
     9961 aaatcagaag agattaaaca agttaaaggc tatgatgtaa tagtgttaag agataagacc
    10021 attcccctta ttgacagtag ggccagcctc aatattgtga ttgaagatga ttcatataaa
    10081 gagaaagatg agatgccggt tgttatcgtt aaatcaggcg gtaaagagat cggacttatc
    10141 gtaggggaac tcctctatca gcaggagata gtaattaaat ccttgagtga ttatctcgat
    10201 aatgtgaaaa atatcagtgg tgctactatt attggagacg gagaagttgc attaattcta
    10261 gatgtaaggg atatagcata aatagaaagg caaggtgtag aaaatggcta atagtgaaaa
    10321 gacagtcgta aaagctgaaa atgcaggtac tagagagcag tatttaactt ttaatgtggg
    10381 aaatgagatt tacggtctta aattaaaaca ggccaaggaa ataattaaac cccctaaaat
    10441 cacaaatgta cctaatacag aagaatacat tttgggagta attaacttga gaggtcaggt
    10501 aatccccgtt ataaacttaa agaataagtt aggcttaaaa gaaaacttca cagccgagaa
    10561 taagaccaaa aagatcgtcg ttattaacat agaaaatatg ttaatcggac tttatgtaga
    10621 tggagtgaac gaagtcatta aactatcagt tgatgaaata gaaggcgttt ctgataacag
    10681 gagcggtatc agagaagaat ttatccaggg ggtatcatct tttggtgatt ccttgattat
    10741 cgtactcgct atcaataact tactttttga tgaaaaagaa gagaaagggg catagttaaa
    10801 atggaagcat atacaaagat ggaagaaagt gcacaaaata atatcacagg ccagtatatt
    10861 aagtttaaga ccggtaacca ggcattcggt atcgatgtac ttaacagccg tgaaatagtg
    10921 agagaagaag agataacctg tatcccggat tccccggact ttgttagagg tgtaattgat
    10981 ttaagagaag agatcgtacc catcgttaac ctgcaggaac tatttaatat taatacctta
    11041 gaagaagagg ttaaagaaaa aaagataatc atcattaaag tagatggtac tttaataggt
    11101 cttcaggtaa acagggtcga tgaaataatt gagatcaata aagagacgat caaagatgct
    11161 ccctcaataa caaagaaata taataaggaa tatatcaagg gtgtagctaa ccatgaagat
    11221 aaactattta tcattctgga tgttaacagg atcttttcag cggaagagat caaaaagatt
    11281 aaagatgtaa aataagacta ttactatgac aggatgagga ggcaaccatg aaaaaagtat
    11341 taatagtaga tgatgctgct tttatgagac taaatcttaa aaatattatt gaagaaatgg
    11401 gctttaagat catagcagag gctgaaaacg gtatagaagc tgttgataaa tataaagaac
    11461 ataacccaga cctggtaact atggatatta caatgcccga aatggacgga atcagcgcta
    11521 caaaaaagat cactagtctg gatgacaacg caaaagtatt agtctgttca gccatgggcc
    11581 aacagagcct tgtaatcgat gccattgaag ccggggctaa ggacttcata gtaaaacctt
    11641 ttgaaaggaa caggattaaa caagctatgc acaagatctt taaataagaa acaaagataa
    11701 tataaatatt ataaataaga aattgcagat aacaagtaat ttaggaggat atggctatga
    11761 ttaatgttct aattgtcgat gattcagcct tcatgcggca gatttttaaa aagaatattg
    11821 aaaaagaatc cgacatgcag gcagttgcgg ttgcccgcaa cggtcttgat gcccttaaga
    11881 agattaaaag atacagacca gatgttatca cccttgatat agagatgccc aagctggatg
    11941 gattagaaac cttaaaagag ttaatgaata aagataactt tgatatcccg gtcctaatgg
    12001 tcagtgccct ggaccagaaa tcaaatgtaa tcgatgcttt agatttaggg gcctttgatt
    12061 ttttacctaa accgtccaac cagatttcaa tgggaattga agagattaca gaagatttga
    12121 ttaaaaagat tcgggctgcc tatgagagta acaagtcaag ggtcaaaaca cgccagagta
    12181 agccggtaaa aagtaaggct accaggaaaa gaattgataa gtttccgatt gtggccattg
    12241 gttcttcctc aggcgggccg aatgctttaa aagaagtcct gaccgcattt cccgagaatt
    12301 ttcccggagg attaatcatt gtccagcaca tgccggccgg ttttacaaaa actttagctg
    12361 agcgattaga taaactctct gaaataaaga ttgctgaagc taaagaggga gataaaatta
    12421 agagaggtaa agccttaata gctcccggta attatcatct taagattgta gatggtgaag
    12481 tgaaattaaa ccaacaggat aaaaaatggg gggtaagacc ttgtgtggac tatatgatgg
    12541 aaacaacagc tcaatattat aaagcaaggg taattggagt tgttttaacc ggtatgggcc
    12601 atgatggagg tattggcatg cgggccatca aagatcaggg cggttactgc attattcagg
    12661 acaagaagtc agctctagta tatggcatgc cgcagtctgt catagatcaa aatgcccatc
    12721 atgaaattgc ttcattaagt gaaattccga tgaaagttat ggagataata gaaaggaaat
    12781 tttaattatg agtgaattcc aagcatttaa acaacaggct tcaaacatat taaatctaga
    12841 cttaggtgga tacaaggaaa agagaataga gagaagaaca aaaagcttaa tgaaaagata
    12901 tgatatcgat gattttaagg aatgttctac cagacttaaa gaagatatta agttcaggga
    12961 ggcctatctc aatcacttta caataaatac ttctgaattt ttccgcaacc cgaaaaatta
    13021 tgtttatcta gaagaaaaga tcttacctaa gttattagaa aacaatgaaa agataaatat
    13081 ctggagtgct ccctgttcca atggggcgga gccctataca attgccatca tcttagaaga
    13141 gatgggggca agaactaacc attactatat cttaggttca gacttagatc ccgaaatttt
    13201 agccgcagct aaaaaaggag tctacagccc aagtgctgtt aaaaatgtgc cggataaaat
    13261 tcttaaaaaa tactttaagg aagtagatgg acagtttaag agataccaac tttcttctca
    13321 gatcttaaat aaagttaact ttgaacagaa agatttaatc aacggtaggt ttagaaaaga
    13381 tgactgggat ctcattatca gccgcaattt ctttatttac ctcacaaaag aatacaagaa
    13441 cttgatgata gataagttta ccaatgttct taaagataat agatacttct ttatcggtag
    13501 tacagaattt atctttggga gtgaaaagtt caatctaaag aaagaaaacc tatcttttta
    13561 taaaaaaatt tctaatcaag cacaaaaaaa ttaaaatttt taattcaaat ttttaacatt
    13621 ccagaaaaaa ggaggggttg agatgaccat ccgcaactgt aaaaagtgtg gtaagatata
    13681 tcagtatgac ggtgttcata aaatatgtat ggaatgccgg cgaaaagaag aagaggattt
    13741 tgagaaagta aaggaatttt taagagacaa tcccaactcg agtataaaca ccgtcagtca
    13801 ggaaactggg gttgaaaaga agacaataat tgaattcatc aaaagcgacc ggttgattgc
    13861 tgaagatatt gaaatagatg taacaatcag atgccagagg tgcggaaaag agataaaaca
    13921 tgggaagtac tgtgataaat gtatatcaga gttaaaagat gaaattgaaa gtattaaaaa
    13981 gaaaaaagaa tccaaaaaga aaaaaaccta tcggagcaga gaaaacatgc atgtaaaaga
    14041 tcgtttcaaa gataattaaa aaatatgaaa aaacccaaaa aaataagtaa tattgactaa
    14101 aaagaagcat tttagagtta atctgtttta attgcaattt ctataattta ataaatgtaa
    14161 tatatatccg ataataaaga tgagaagtga taaagttgcc aaactatagc aaaaggagtg
    14221 atttttcatg aagattaata ataatgatat taacatgaat aaaatcatga aagcatacca
    14281 gaatatgaac cagaataaaa acaagcaagt agagcagaaa aaaagaactt ctgatcaggt
    14341 taatatttct aaggaagcaa aagatattaa gaagatgcag aagtctttac aggaaaaacc
    14401 cgacatcagg cgtaaaaaag tagaagagat taaaaaacag gtcagcaatg gaacctataa
    14461 agttaacccc gaaaagatag ctgaaaagat agtctctgac ttagaagagt agaggtgtaa
    14521 aaatgtccga aaaactatta tccgaattac tggaaatttt aaaagaagaa aacaacttat
    14581 ataagaacct tctcgatata gctgaagata aaaaggaaac tctgatcaat aatgaaatag
    14641 ataccctctt ttcccatgta gaaaatgata gagaccatat cgatgagatc tctaacttag
    14701 aagatagaag gttggagctc atggaagata tcaaagagga ctttgatatc gaggatgatc
    14761 tttcctatct ggaatttgtc agccaattac cagacgaatg ggaaaacagg ttaaatcctg
    14821 taagggaaga tctattgact acccttgagg agtttcatgt tataaatgaa gaaaacaaga
    14881 aactgattga agaagcagta aggtttaata aattttcgat cgatctaatt gtagataatt
    14941 taaagaataa tgaaaacaca taccacagcc agaatgaaag ttctccaagg ctgttagata
    15001 caaggggcta atacagatag ggaggtaagc catcttatgt tcgatggaat taatatagga
    15061 ttaagtgcac tacaaaccca gaaaaaatca atagaaacca caggccataa tatagccaat
    15121 gccaacaacg aagattattc caggcagaga gttatccaga ctgctaaaga gcccaatata
    15181 ttaccgggca gtaactttat gcagggaacc ggtgtaaaag tggaagatat ccggaggatc
    15241 caggatcagt ttatcaacca gcaggtaaga gaagagtccc aggaatccgg ttactggaaa
    15301 cagattcaat caggtatgga aagaattgaa tatatcttta acgaaccatc tgattcaggc
    15361 ctgcagagtg cctttgataa attctggaat tcactgcagg atctaaatac ctatcccaat
    15421 gatacagcta ccagagctgc tgtaaaaaac cagggcatgg tcctgtccaa agcctttcgt
    15481 aatatcaacg aacagatgat ggattacaag cgctccctaa acagtgacct cgagaaaaaa
    15541 gtaagtgaga tcaattcaat tgctgataga atcagtagat acaacaaaca gattacaaca
    15601 gccttagcga gtaataatac tcccaatgac ttattagata aaagagaagc cttatttaat
    15661 gacttaaatg aattaacgac agttaagggt aaaaccgata aagtaggtaa tttaaatatt
    15721 actatagatg gtaaaaagtt agtaagtaag tttgatacca ataaattaga agttgctgaa
    15781 agtgataata aaccatatgc agaccaggta attatagata tcagcggcaa caaagttaat
    15841 attaaacctg agaccggtga aataagaggt attatggatg ttagagatca ggaaatccca
    15901 aattatttag ataccctaga tgatatagca aaaagttttg ctgacaaatt caatgaaaaa
    15961 cacagcaatg gttttgacct taaaggaaat accgggaatg aattttttga attaaacagc
    16021 agttattcca gaccttcaag agctattcag gtgcattctg ctataaaagc agatggaggt
    16081 ataaataaaa tagcagccgg taactatagt gaaaacccgg gtgtgtcttc agttgaaata
    16141 acagaaccag gtaatattga taaaaaccaa tattataaag ttgaagtatc aaaatcggga
    16201 gatcagtatg attatgaact ttttgagaaa gatgccggtg accctacttt cagctcactg
    16261 gttacaactg gttcgcttga tatagagacc gctgctgaaa atccggttga tgttagttcg
    16321 cgcggttttg aattcacagt taagggaaac ggtgagacag taattaattt caatgaaaat
    16381 cagggtaatg gaaataatgc tgcttccctg gctaaaacaa ttaatcagga tgaaatatta
    16441 aatggggcaa cagtaagtga taattataaa gcattaattt caacagtagg agttaatgcc
    16501 cagagagccg atcagatgaa agcaagccag ggagacctgg ttaaacagtt agagaatata
    16561 aagaaaagtg tttccggtgt ttctttagat gaagagatgg ccaatatgat taaataccag
    16621 caggcctata atgcagcagc aaaattaata accaaaaatg atgaaatgct ggacagtcta
    16681 atggctatga ttagatagga gtgaaagtat gagaattaca gatggtatga tggtaggcaa
    16741 catgcttaaa aacttacaaa ataatagatc tgaattggat aaactaaacc agcagttatc
    16801 ttcaggtaaa cagtttagta aaccttctga tgatccgatt ggtgtgacca caagtatggg
    16861 ctttcatacc aagatgaaca actatgaaca gtatcaaaaa gatgtaaata atgccaaaag
    16921 ctggttagaa tccacagaaa attccttaag tgatgctggg aaaatattgc aaagatcaag
    16981 tgaattagca atatacgggg ccaatgatac cttaaatccc gatgacagaa aaaacatgtc
    17041 agaagaggta aaagaactaa ggaatgaatt aatcagtatt gccaatagta aattgggtca
    17101 ggactatctc tttgccggac aggcctcagg cacaaaacct attgaagtaa ataatgagta
    17161 tcatactaat aatgatttaa cacttaataa tcaaaacaat attagttcag ttaagagaag
    17221 tagttccacc gctttaaccc ccggtaatta taccgtttct ctagaagaaa acggaggcac
    17281 agttgataca attaatatta gtaatgaaga gggagaaatt gttgcttcta aaactaatga
    17341 tactgctttg acacaagata cagagtatga agttgaaact gataatgggg aaaagttgac
    17401 tattaaaaca ggcaacaata atcccacagt tactgaccag gcggagtttg aaattcattc
    17461 ctatgttaaa tataaagggg accataataa gatattaaga aaaattagcg atgataacaa
    17521 catgaaagtt aatgctaatg ctgataaggt atttaaacag aatattgaaa tagtaaacaa
    17581 tttatatgaa gatctaaaag cgggtaaagg tggagaaaaa atatccgctt atgctactaa
    17641 actgcaaaat ggaatgacaa ataatacagg agcaagagcg gaagtcggag caaaaattaa
    17701 taggctggac ttaattacaa atagaattga tgatgaacta ttaaatacag agaaactaaa
    17761 ttctaaaaat gaagatatag atttagctaa gttggtaact gatttaaaga tgagcgaaaa
    17821 tgtctataga gcttctttat catcagcagc aagggttatt caaccttctt tagttgactt
    17881 tttaaagtaa gatcttaaag attaatttgc gaggtgtaaa aaaagtggaa atattaacca
    17941 gggattttgg aaaagtaaat atagatgagg ataaaataat agaatttcca gatggactac
    18001 cagggtttaa agaggaaaaa gaattcgtct tattaccttt agatgaagat tcattatttg
    18061 taataatgca gtctgtaaat accccagaac ttgcctttat tactattgaa ccaagaaaca
    18121 taataaaaga ttatgaattt gtcatttctg aaaaaacaga agaattacta caaatcgaag
    18181 gtattgaaga tattatactt cttaatattg ttaatattaa agataaactc caggatatga
    18241 ccattaacct agcagctcca ctggtaataa atatcaacaa aaacttaggc aaacaaatta
    18301 ttctcgatga cagcaaatat cctgtaaagt ttaaattaga aaataatgaa gcaaaagaga
    18361 tgatgtccta atgcttatcc taacccgaaa agaaaatgag aaaatcttaa tagatgaaga
    18421 aattgaaata tctattgtgg agatcaaggg caataaggta aaaattggaa taaatgcccc
    18481 ggaagaagtt gagatcttaa gagaagaagt atataaatcc gtaaaagaag aaaacattga
    18541 agctacaaaa gatagaaaag ttaacctgga taaattaaaa ggatttggta aaaattcaaa
    18601 aaatagtaaa aaatagagca tttgagagta attagtagat aaatcaaagg aaaccatata
    18661 tcaaggtctt taatttaact agagtgttat taacaagatt tttatttagt taactaattt
    18721 aagaataagc cgataatata attagaagat aaataaagta tttagtaatg attataggcc
    18781 tgaatttatc agtcctataa tggttactaa aacccgtaac cagcatcac
//
LOCUS       DATGCJ010000247         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_574168, whole genome shotgun sequence.
ACCESSION   DATGCJ010000247 DATGCJ010000000
VERSION     DATGCJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574168"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VKN64_10990"
     CDS             <1..693
                     /locus_tag="VKN64_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMA60802.1"
                     /translation="SEAFAQDKNQLIKRRTETALRLTILIGLPSAAGLFILAEPLTSV
                     IFNNAQAAIPLRIVAWGVFFIAIQQSTSGIIQGIGKTKIPARNLFIGALVNGGLNYYL
                     TALPAFGIKGAAVGTVTGFAVAAGLNLISLKYYTDFKFKVKQFIVKPVITVAIMVVFT
                     HYGFGILNNYLNMLQIPSAYIISTFLIVIMAVIVYMATLIIFKEIKYSDLKMIPYVGD
                     HIAEFLRKYNVL"
     gene            706..1500
                     /gene="mazG"
                     /locus_tag="VKN64_10995"
     CDS             706..1500
                     /gene="mazG"
                     /locus_tag="VKN64_10995"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMA60803.1"
                     /translation="MESKNFESEFDKLLYVMDKLRGPEGCPWDRKQDFYSLKPYIIEE
                     AYEVVEALEEKDMELLKEELGDLLLQVVFQAQIGKEAKIFDINDVISGITTKLIRRHP
                     HVFGEEKIDTAQEVKKTWEEIKKKERTNNNEGSILDDVSKIQPSLNQAYEIQQKAAEV
                     GFDWDEIKDVLLKVEEEIAELKEIIDKENKDRIEDEIGDLFFAVVNLSRFKDINPEIA
                     LLRTINKFKKRFNYIEDTVKDENKNIRQMSLEELDGYWNEAKEKNL"
     gene            1512..2672
                     /locus_tag="VKN64_11000"
     CDS             1512..2672
                     /locus_tag="VKN64_11000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HMA60804.1"
                     /translation="MKRFTTTIIFILVFVLSLSAIAGAQDFEIDAESAILIDAASGQV
                     LFKQNPDERLPPASLTKIMTLIIAMEEIESGDLSLDDQVTISRLAESMGGSQIFLGEG
                     NKVKVRDLLKAVTIASANDACVALGEAVAGSYNKFVNMMNEKADELGLENTYFSNSTG
                     LPTESDHYTTARDVAIMSREIVKYPLILKDASIWLDYIELPDRKAMLTNTNHLVNTYP
                     GLDGIKTGHTNAAGFSLAATAKRGDMRLISVVMKARSVKAREDQTARLLDYGFNRFTR
                     KNYLEKGEQIQNIALPNGAKEYTTGKVGQDLAVVVKRGQEDTISTEVNIMEEYNYPID
                     EGDKLGEAKALRDGEVITKVNIVATEKINKANFLALFWRNFVNWLGGRMGSS"
BASE COUNT         1058 a          411 c          627 g          869 t
ORIGIN      
        1 tcagaggctt ttgcccagga taaaaaccag ttgataaaaa ggagaactga aacagcctta
       61 agattaacca tcttaattgg actgccttcg gccgctggac tatttatttt agctgaacct
      121 ttaacctcag tcatatttaa taatgcacag gctgccatac cgttaagaat tgtggcctgg
      181 ggggtattct tcatagctat ccaacagagt acttctggta ttattcaggg gatcgggaaa
      241 acaaaaatac cagcccgcaa cttatttatc ggtgctttag ttaatggtgg gttaaattat
      301 tatctaactg ccttacctgc ttttgggatt aaaggtgctg cagttgggac tgtaacaggt
      361 tttgcggtag cagccggttt gaatttgatt tctttaaagt attatactga ttttaagttt
      421 aaagtaaaac agtttattgt aaaaccagtt ataacagtag ccataatggt tgtttttacc
      481 cattatggtt ttgggatatt gaataactat ctcaatatgt tacagattcc cagcgcttat
      541 attatctcaa ccttcttaat tgtaataatg gcggtaatag tatatatggc aacattaatt
      601 atatttaaag aaattaaata tagtgatcta aagatgattc cttatgtcgg tgatcatata
      661 gctgaatttt taagaaagta taatgtactt taggagagat tattaatgga aagcaaaaat
      721 ttcgaaagcg aatttgataa actattatat gtaatggata aattacgcgg tccagagggc
      781 tgtccctggg atagaaaaca ggatttttat tcattgaaac catatataat tgaagaagcc
      841 tatgaagtcg ttgaggcatt agaagaaaaa gatatggaat tattaaaaga ggaattaggg
      901 gatttattat tacaggtggt ttttcaagct cagattggca aagaagccaa aatatttgat
      961 ataaacgacg taatatcagg catcacaacc aaattgataa gacgacatcc acatgttttt
     1021 ggggaagaaa aaattgatac cgcccaagaa gttaagaaaa cttgggagga aatcaagaag
     1081 aaagaaagaa ctaataataa tgaagggtca atattagatg atgtttcaaa aatacaacca
     1141 tctttaaatc aagcctatga gattcagcag aaagcagctg aagttggttt tgactgggat
     1201 gaaattaaag atgtactctt aaaagtggaa gaagaaatag cagaattaaa agaaataatt
     1261 gataaagaaa ataaggatag gatcgaagat gaaataggtg atttattttt tgctgtagtt
     1321 aatctatctc gttttaaaga tataaatcct gaaattgctc ttttaaggac tattaataag
     1381 tttaaaaaga ggtttaatta tattgaagat actgttaaag acgaaaacaa aaatatcagg
     1441 cagatgtctc tagaagaatt agatggttat tggaacgaag caaaagaaaa aaacttatag
     1501 gaggaagtaa attgaaaagg tttaccacta caataatatt tattttagta tttgttttaa
     1561 gtttatcagc aattgctgga gcacaggatt ttgagataga tgctgaatct gctattttaa
     1621 tagatgcagc cagcggccag gtcttattta aacaaaatcc agatgaaaga ctaccacctg
     1681 ctagtctcac taagatcatg acattaataa tagcaatgga agaaattgaa agtggtgatt
     1741 tatctttaga tgatcaagtt acgatcagcc gtttagctga atctatgggt ggttcgcaaa
     1801 tatttttagg ggaaggcaat aaagtgaagg ttagagattt attaaaagca gttacgattg
     1861 cttccgctaa tgatgcttgt gtagccctag gagaagctgt ggcaggttca tataataaat
     1921 ttgtgaatat gatgaatgag aaggcagatg agttaggatt agaaaatact tacttttcaa
     1981 attcaactgg tttacccacc gagtctgatc actatacgac agcacgtgat gtagcgatta
     2041 tgtcccggga gatagtcaaa tatccactca tcttaaagga tgcctctatt tggttagatt
     2101 atattgaact accggataga aaagctatgc tgaccaatac aaaccatctt gttaatactt
     2161 accccggttt agatggaatc aaaacgggtc atacaaatgc agcaggtttt tccttggcag
     2221 caactgccaa acgaggggat atgagattga tctctgtcgt tatgaaagcg agaagtgtca
     2281 aagcccgcga ggatcaaacc gctagattac tggattatgg ttttaataga tttaccagga
     2341 aaaattattt ggaaaaaggt gagcagatac aaaatatcgc cttaccaaat ggggctaaag
     2401 agtataccac aggtaaagtt ggccaagatt tagctgttgt agtgaaacgt ggtcaggaag
     2461 ataccattag tacggaagta aatataatgg aagaatataa ttatcctata gatgaaggag
     2521 ataaattagg tgaggcaaaa gcactaagag atggagaagt tattaccaag gtaaatattg
     2581 ttgcaactga aaaaattaat aaagcgaact tcttagcatt attttggcga aattttgtaa
     2641 attggctggg aggaagaatg ggtagtagtt aagatctatc aaataatcaa atagacctta
     2701 gaccacacct gctaaagggt gtggttattt tttctaaagt ttttttgaga aaatgcggtt
     2761 ttccccttga cagagcgggg gttagttgat aagatataaa atgtcgctga gggaaaaagg
     2821 cgtcaaaaaa aagaggccaa aaaccccttg acagcggccg cgaaaagtgt taatattatt
     2881 tcgcggtaga gaaatagaac cttgaaaagt gaacagcaga ccctgacagt tcttaataag
     2941 tttcaagtaa gaactttcaa tagcg
//
LOCUS       DATGCJ010000248         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_307821, whole genome shotgun sequence.
ACCESSION   DATGCJ010000248 DATGCJ010000000
VERSION     DATGCJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307821"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VKN64_11005"
     CDS             <1..780
                     /locus_tag="VKN64_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60805.1"
                     /translation="IVLKGNEIGREYSLDGAVGAGTLYLDSGLNYSRSINKLKEQFKA
                     EEVSIGATLHYLKGGIAHIKGDGSFIIDYEDAISGSGNLKVEYAEDAAGYALGFGINT
                     KVNEKLAWGASISNIGKLTADKSRYFEYSYDPIEEEFTETPEQEGGKLEYTLPLKFNL
                     GTKYKWKANTNLIGGYSLTNYSTSYSDHKISGGVEYNRIKFLPVSFGVTYSSLQGSII
                     LSSGAALKLGPMYANVTFSDLQALFNGSKSISFGISTGFSF"
     gene            973..2766
                     /gene="typA"
                     /locus_tag="VKN64_11010"
     CDS             973..2766
                     /gene="typA"
                     /locus_tag="VKN64_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMA60806.1"
                     /translation="MLNNKIINLAVIAHVNAGKSTLVDAFLNQSGIFRENEVVEEFIM
                     DSNDLEKERGITIYAKNCSIEHDGYKINIVDTPGHADFSSEVERIIKAVDTTILVVDS
                     SEGPMPQTRFVLKKSLEQGIKPILFINKMDKNNERAKEVEDMVLDLFIDLQADDQQLE
                     FPIIYGIAKDGIAKYDIDGDSENLDPLFETIINHVKPYPDLDNQPLQIQVHDLAYDDY
                     LGRIGIGRIYSGSIENRQQVEVSKRDGSIKKEKVTELLGYHGLKQSSIEKAGSGDIVA
                     VAGIPHISIGETICDVGEVKPMDMIEIEEPTLSMNFSINDSPFSGESGKYLTSRNLKS
                     RLEKETEVNVGLNVEKLEGSEGFKVSGRGELHLSVLLEHMRREGYEISVSKPEVLYKE
                     ENNKLMEPIERVMVEVPEQYSGTVIEKLNQRKGLMQYMKKDNDYIKLNYLVPTRGLIG
                     YKSEFINDTSGEGTLIRSFERYEEYKGAIPKRTNGVLVSQNEGKAMPYALDNLSARGT
                     IFIKPGTRVYEGMIIGMNNKDNDLTVNPCKNKKLTNTRAAGSDDAVNLAPPKTFSLEE
                     ALEFINEDELVEVTPDAIRLRKKILRGKLRK"
     gene            complement(2846..3733)
                     /locus_tag="VKN64_11015"
     CDS             complement(2846..3733)
                     /locus_tag="VKN64_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMA60807.1"
                     /translation="MDTFFAKIKRSIPIQVLDTIDIEEYSSSKISIIKVKNAFSAPNS
                     FAMFKFILPLTKDYPFEIVEKNYYQLKKNHLFPINPGQLHCSETYGEPIIEIKPFLAI
                     FIDKNFMQMMSKKLFNNREVIFDNINNSITVDFRNILNKFIEEINNKPVGYELMIENL
                     DKQIAISLLRDLDNNLNKKFLNTDQSDKKSINKAIYFINNNYDKELSLEEVAATANYS
                     PYHFIRVFKEETGKTPFQYLLDIKIDKAKEKLYNSNKTISDICYECGFNNRSHFSFIF
                     KRKTGNSPSEYREIIANSS"
BASE COUNT         1425 a          580 c          689 g         1194 t
ORIGIN      
        1 atagtactaa agggtaatga aataggcaga gaatatagtt tagatggggc agttggggct
       61 ggaacactat atttagattc gggccttaat tattcccgtt ctatcaataa actaaaagaa
      121 cagtttaaag cagaagaggt atcaattgga gcaactcttc attatctgaa gggcggaatt
      181 gctcatatta aaggtgatgg ttcatttata atcgattacg aagatgccat ttccggttcc
      241 ggtaatttaa aagtagaata tgctgaagat gctgccggtt atgccttggg ctttggtatc
      301 aataccaaag taaatgaaaa acttgcctgg ggggcatcaa tctcaaacat aggtaaatta
      361 acagctgata aatctagata ttttgaatat agctatgatc ctatagaaga agagttcaca
      421 gaaacccctg agcaggaagg cggtaaatta gaatatactt taccacttaa atttaacttg
      481 ggtactaaat ataaatggaa agccaataca aatttaatcg gaggttattc tttaacaaac
      541 tacagtacta gttattccga ccataaaata tcaggaggtg ttgaatataa taggatcaaa
      601 ttcttaccgg ttagtttcgg agtaacatat tcttccctac aaggcagtat aatactttcc
      661 tccggagcag cccttaaatt agggcctatg tatgctaacg taacattctc tgacttacag
      721 gccttattta acggttcaaa aagcatctca ttcgggataa gcacaggctt ttctttctag
      781 attaaatata aataaatact gtcctcaaac cccgcttttt ggcggggttt ttaatatttc
      841 acacaccctt tatctcaatt aaagacctca atctaatata gtaattaaaa aaatagcttg
      901 attctaacta cagataccta taaaatagaa aatgttgcat gtaaaaagaa agacagaaag
      961 aaaggaatgt ttatgttaaa taataaaata ataaatttgg cagtaatagc acatgttaat
     1021 gccggtaaat caaccttagt agacgccttt ttgaatcaaa gcggtatatt tagagaaaat
     1081 gaagtggtag aagaattcat tatggacagt aatgaccttg aaaaggaacg cggtataaca
     1141 atttatgcta aaaactgttc aattgaacat gatggatata aaataaatat cgtagataca
     1201 cccggtcatg ccgatttttc ttccgaagtt gaaagaatta tcaaagcggt tgataccacc
     1261 attttagtgg ttgattccag tgaaggaccg atgccccaaa ccagattcgt tttaaaaaaa
     1321 tcattggaac agggtatcaa accaattttg ttcatcaaca agatggataa aaataatgaa
     1381 cgggccaaag aagtagaaga catggtatta gatcttttta tcgatctcca ggccgatgac
     1441 caacagcttg aatttcctat catctacgga atagcaaaag atgggatagc aaaatatgat
     1501 atcgatggtg atagtgaaaa cttagatccc ttgtttgaaa ccataatcaa ccatgtaaaa
     1561 ccctatcccg atttagataa tcaaccttta caaattcagg tccatgacct ggcctatgac
     1621 gactatctgg gtagaattgg tatcggtaga atatatagcg gcagtataga aaatagacag
     1681 caggtggaag tatcaaaaag agatggcagt ataaaaaaag aaaaagtcac tgaactactg
     1741 ggttatcacg gtctaaaaca aagctcgata gaaaaagccg gtagtggaga tatagttgcg
     1801 gtagctggta ttccccatat ctctattgga gaaactatct gtgatgtagg agaagtaaaa
     1861 ccgatggata tgattgaaat agaagaacct actctatcaa tgaacttttc aatcaatgat
     1921 tctccctttt caggtgaaag cggtaaatac ctgacttcta gaaacctcaa gtcaagactt
     1981 gaaaaggaaa cagaagtaaa tgtgggatta aatgttgaaa aattagaagg tagtgaaggt
     2041 tttaaagtct cgggccgcgg cgaactgcat ctatcagtat tattagaaca catgcggagg
     2101 gaaggatatg aaatatcggt ctccaaaccc gaagtactgt ataaggaaga aaataataaa
     2161 ttgatggaac cgattgaaag ggttatggtg gaagtaccag aacaatattc aggtactgtt
     2221 atagaaaaat taaatcaaag aaaaggctta atgcaataca tgaaaaaaga taatgattat
     2281 atcaaattaa attatctagt accaactaga ggattaatcg gttataaaag tgaatttatt
     2341 aatgatacca gtggagaagg taccttaatc agatcatttg aaagatatga agaatacaaa
     2401 ggagccatcc cgaaaagaac caatggtgta ttagtttctc aaaatgaagg taaagccatg
     2461 ccttatgcct tagacaattt aagtgcacgg ggaactattt ttattaaacc aggtacaaga
     2521 gtttatgaag gtatgataat tggaatgaat aataaggaca atgatttaac cgttaacccc
     2581 tgtaaaaaca agaaattaac taacactcgg gcagcgggat cagatgatgc agtcaatctt
     2641 gccccaccga aaactttttc tttagaagaa gccttagaat tcatcaatga agacgaactg
     2701 gttgaagtaa cacccgatgc tattagactt agaaagaaaa tattgagagg caagttaaga
     2761 aaataaatta aaattatata ggattaatat taaaattccg ggtttccccg gaattttctt
     2821 attttgaata gaaatatttt tttaactaac tactatttgc tatgatttct ctatattcag
     2881 agggtgaatt accggttttc cttttgaaaa taaagctaaa atgactacga ttattaaacc
     2941 cacactcata acaaatatca gaaatggttt tatttgaatt ataaagtttt tcttttgctt
     3001 tatctatctt tatatctaat aaatactgaa aaggtgtttt accggtttct tctttaaaaa
     3061 ctctgataaa atgataagga ctataattag cggtggctgc cacttcctct aaagataact
     3121 ccttatcata attattattt ataaaatata ttgccttatt aattgatttt ttatcacttt
     3181 gatcagtatt taaaaacttc ttatttaaat tattgtccag atctcttagt aaactaattg
     3241 caatttgttt atctaaattc tcaatcatca attcatatcc taccggttta ttatttattt
     3301 cctcaataaa tttattaaga atattcctaa aatctaccgt aatagagtta ttaatattat
     3361 caaaaatgac ttctctatta ttaaataatt ttttactcat catttgcata aaatttttat
     3421 ctataaaaat agctaaaaat ggtttaattt caattatggg ttccccataa gtttctgaac
     3481 agtgtaattg gccgggattg ataggaaaga gatggttctt tttaagttga tagtagtttt
     3541 tttcaactat ttcaaacgga tagtcctttg ttaaggggag aataaatttg aacatagcga
     3601 atgaattagg ggctgaaaat gcgttcttga ctttaattat tgatatcttg ctactggaat
     3661 attcttctat atcaatagta tctaatactt gaataggaat cgatcttttt attttagcaa
     3721 agaaagtgtc catataattc cccctttaat gtgtattatt actaaattat agtaaaatta
     3781 tacagcaatt aaagtaaaat atcaagcaat taaactaaca tattaagaca gaataactgg
     3841 taaaatatag ttagagacca taaatttatc aataatgagg ggggagaa
//
LOCUS       DATGCJ010000249        13474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_131086, whole genome shotgun sequence.
ACCESSION   DATGCJ010000249 DATGCJ010000000
VERSION     DATGCJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131086"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VKN64_11020"
     CDS             complement(<1..668)
                     /locus_tag="VKN64_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMA60808.1"
                     /translation="MDKVKVGVIGVGSMGKNHVRSYASLKHVCDLVGVYDIDQKRAHE
                     MAKSYGVKSFSAVEDLLQEVEAVNIATPTTTHYDIALQAIDAGKHILIEKPITNKIDE
                     ANEILKRAKDKKLIVQVGHIERFNPAIKALPEMLKGEKIVAIDVQRMGPYDPRIDDTD
                     VIQDLMIHDIDVVSSLIDSEIVNVNAFARSVASEKHMDFAVANYQTEDDVIVTLTSSR
                     ATNK"
     gene            complement(688..1473)
                     /gene="lpxA"
                     /locus_tag="VKN64_11025"
     CDS             complement(688..1473)
                     /gene="lpxA"
                     /locus_tag="VKN64_11025"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMA60809.1"
                     /translation="MLKNNKIDDTAIISDTVELGENIEIGPYTIIEEEVEIGDNTKIG
                     ANCHIQGWTKIGENNKIDNYVTIGFPPQNIGYDGSKTYVIIGDNNQIGDYVAIRRGTT
                     EGRGETKIGDNNTFKVYSHVAHDCSLGNNITLENYVNLAGHVTIYNNAYLEEMVGVHQ
                     FVKIGNLSRIEGHSKLIKDLPPYIIVNGHPAKVVGINEVGLNEKNIDPDVRDEIRKSY
                     HILYESNLNISQAIEKLEKVSKNFMEVKDFINFLKNSNRGICR"
     gene            complement(1569..2222)
                     /locus_tag="VKN64_11030"
     CDS             complement(1569..2222)
                     /locus_tag="VKN64_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60810.1"
                     /translation="MQKSTIIFILLVLCFTNPAAADFELSGKLERGENTYLSNQDLLS
                     ENVVDYYSYDNFWLKLKKKLDYPNYYYFKVKYYEKLYDEESTYDNRSLDLTGNYTQEF
                     NEIFRNKFKFKLKDKRYINNRDNSYTSYSFGYQFRHTVNEKNQYSLDLKKKEYSYVNE
                     YRKNYLINTYKINWQRDISDKFEIELGYQLKNKEYQYSTDSNDRVVQKYSIDFSYDL"
     gene            complement(2246..4033)
                     /locus_tag="VKN64_11035"
     CDS             complement(2246..4033)
                     /locus_tag="VKN64_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60811.1"
                     /translation="MKKYIKYISIFLICFIFLFTAPLLAQDDNLEIGNIDDQVSNIEA
                     DNIYINTHKEEINAVGNAKFINPQVEINAKKIVFKYADQIMTAVGKPLILKYEGRIIK
                     GKSLTLDYEKGVANLSQASLDIDKFQFEGEDVDYLKDQNPNIIVKDAFYTTCIMDEPH
                     YHYTAKNIQYYPNDKIVGKNITFWWSGNRLITLPRYVVNVTTDEEGKTTVDNSFPVPE
                     LGYNLEQGLYIELVYPYEFSTNNYGDLYYIREDKENINFDWNHKYILDSDKYIFFDFQ
                     QQRYLDNDEIVNEEREIKLGFDHKINENIDYRVYARDYEKLKPIPEEQRQTSLNFAVD
                     YTRKKYNINADFGYDFRNEDRIEKVSTTYTPIKNLRTETYHEFTNEKLQKQKYSISQH
                     LNKLGWMIKYLKGYNTDYLPYGNIGYSFNKDLDLSIGYGLVKEGAIKEYKTDYELDYN
                     KNIKITKNFNIGFKQKIKHVNYPKADEKLTNYETAIYFNLDKRISKNLNLDQRLGYSI
                     KDQEGTPLFSIDELDKDKVITSNTELDLYLPKNLEHWKINLDLAYSIPDQEFDTQKIG
                     VTHEYDCYSYQINYNHIDKAIGFEFSFIN"
     gene            complement(4127..5041)
                     /locus_tag="VKN64_11040"
     CDS             complement(4127..5041)
                     /locus_tag="VKN64_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60812.1"
                     /translation="MNKKRNIIIFLIIISLFNFNVLTATAQLVPVEKLSAVEIILYGQ
                     KSDKSILERVERLEDTLYKEKKEGSLIERANNIYNYVLNNNDSPSLLFDLNGLEWSLT
                     TDITQDTIINKLNRLEKMVFGESKDGPLIERINYLTKVTFPDETVPAKQIKIPKNTLI
                     KIKTFDKVSSVSSKSGDRVRFEVAEDVYIDSKLAIPAGSTGIMEISQVEKSGNLGKEG
                     KIDLNFSKVMAIDGTEVRVDLDEEAQMMNESQQLALGASILGAAVLGPVGLIAGYFVK
                     GKEEELPKGSEIYVQTVNNNNVYGLNIE"
     gene            complement(5063..6118)
                     /locus_tag="VKN64_11045"
     CDS             complement(5063..6118)
                     /locus_tag="VKN64_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60813.1"
                     /translation="MNRKIILYFVLVLMFTVSLNIFAQEVEQNVNDNLSNSIFYSPDY
                     NSIDINDYILRLPEDDMVLEDFGAQYQNKLKELANRYGSNQNNLLDIVFSNEEIENTE
                     PQENLIIDPSSRLNINFGYDQEDKNEIVENKTAIQVNYNLDDKTALSAEYELQNTQEY
                     DVGLNNIDTMNLEENSQLLRDENNIDTRLGISYQTTDNIRIFADYVYNDILLEQSGES
                     TIFGLEYNTNDNIIAAEYSIIDELETQGRETGLSYQYEDLAKFSASYKLLDPKTIKDQ
                     LKQEQSWDFGVDFNLNEDSSFSLGFRVINKELLDANNNIEPTSNTDDTEDGTNNNSNN
                     SESVEETNVEASFQIKF"
     gene            complement(6327..9191)
                     /locus_tag="VKN64_11050"
     CDS             complement(6327..9191)
                     /locus_tag="VKN64_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HMA60814.1"
                     /translation="MLVVALATPAIGASFPDVPSNHWAYEAINELVAAGVIEGYPDGT
                     YKGQRNLTRYEIAMIVSRVMDNMAEQRQELMDKVESMEKKDSGLTTEQAQDVTAIVKA
                     LMEKNMPEAPEPPTELTDQQADQVVNLIEALTFEYQAELKVLRSDYDSLRDDLGLLED
                     NLESVRERVTALEEATPPVTFGGSYSVTFNHKDVIDGEVIDTGSGFNHYYIEDAPLSA
                     YDADGDLALDDDEFEAYVSDVAAIIETRIREMNGEIDSDPANNYEVVAEYYNHVFDDT
                     LEEDEVTESTLVELAVAEFNYVLVGQPTEVGSYTGTIPGGEITAAEVESNTEWSTVST
                     SYTLADFRIQEDTADAVDALTMWDDGDADAMEFEEIAELTHTLNLNADINYAGFEGQV
                     GVTLEKLDSTDEATFKSADLELENDELKMVYNEKNEVSYSDFAVSSEKFNAASFLYKP
                     YGVNAFVGIDEVEVDDSVNPLTNDSEDDEELNVSWEDYDWADADEDDVYELYFNGDAT
                     SDTADYVVAGAQKTFVLDMADVKTTVAGRLSPDFEESADDEYEDLVVGAEATAKISGF
                     DFAGDVAVSTDAVTDEFGYLFRVNASTQLVDAFTAGASYKNRGENFSPLYGSLSVFDD
                     EDTGYNKYEAGLAQEKMSGYGVSVAHDGTAENALVSGLDLNFGYDVVDYDGYDENDTR
                     LSAGATMAVMENLTVEGSFKQFTEPTGEETDYDGDTTKYSVGATFVPVENAEVSAAYK
                     VMDPVEDVIPTEDDDGEEYKAVNENTIELKGSYDFTDYVGVTGSYTDVSGLSNYEDAE
                     KTTTSVGVSLTDYPVITGLTASASVDYKMVTGKEFVDAEETANPDEDFNVLDATDTPG
                     FEEYDATTTSMKAGLAYTLNEKTSLTYDLGYTTQEAKDEASDLYSGNYMTNAVGVDYT
                     FTENASLTASYKLMQMDYDADDGADDWTAQEATAGVSISF"
     gene            complement(9617..11347)
                     /locus_tag="VKN64_11055"
     CDS             complement(9617..11347)
                     /locus_tag="VKN64_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HMA60815.1"
                     /translation="MSLENKSEIYEKIKGKLIALYGEEKGESVFARLYERMKEFKAKN
                     PAISEKVYNKDILTEEDVVLITYGDQVKDGDDTLDVLHRFLNKYVKGYINIVHILPFF
                     PYSSDDGFAVIDYKEVNEEMGDWTDIEEISEDYNLMFDAVINHISSHSEWFQKYKEGH
                     PEYLDYFIEVDPDVDVSKVTRPRAKPLLTEVETNRGKKHVWTTFSPDQIDLNYKNEDL
                     LLNVIDILLFYVKKGASILRLDAIAYLWKKIGTSSIHLKETHEVIKLFRCILDEVAPE
                     VLIITETNVPYEENISYFGEEEREADMVYQFTLPPLVLHTFHTGDAQKLIDWAQDLKE
                     FGLGKYYFNFLASHDGIGVRPVEGILTNEEIDSLATRTKENGGFVSYKTNNDGTKSPY
                     ELNIKYFDALVQEEEPMDLKIQKFINAHSILLTFRGIPAIYIHSLLGSRNYIEGVKLT
                     GEKRTINREKLSLEELETSINEEGTIRHQVYENLTEIISIRKEQSALHPNADQKVLDI
                     DERIFALLRENRETGEQLLALHNISKEKIEGINLKKILKNREEFEDILKNNKMENIID
                     LKPYDFKWIR"
     gene            complement(11384..12709)
                     /locus_tag="VKN64_11060"
     CDS             complement(11384..12709)
                     /locus_tag="VKN64_11060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMA60816.1"
                     /translation="MKIAMIHFRTKQTDGVSLEMDKWRDVLEDLGHQVIYITGSKDEK
                     EVYLPELEYRNERNLKLVEEIFKKENPSKTEEQIKEDIYNYANQFKTKFKKIIKKRDI
                     DLLIPNNVFSLGWNIAVGFGLAQAIEELELAAIAHHHDFYWERELYQDPNYKIVELML
                     EKYFPPKLASIRHVVINRIAQKKLMNKKDIWSEVVPNVFDFKQDHWKINEDNKDLKRE
                     LGLKENDIIFLQATRIAERKAIELAINFVSKFKRQYKEKLINNNLYDNRQFRKENEIV
                     LVMPGLEEDNESYISKLKKLANEKGVRIIWAHQLLKENRYSLWDFYANCDMITYPSIK
                     EGWGNQLLEGFFSKKPIMIYEYPVFKTDIQKYNPIVASLGSGYESKTNGLVSIEEKRY
                     KEPLEEAYKYLTNNEYRQEAVNYNFVIARRNFSKERLKEILEKLLSNFI"
     gene            complement(12737..>13474)
                     /locus_tag="VKN64_11065"
     CDS             complement(12737..>13474)
                     /locus_tag="VKN64_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HMA60817.1"
                     /translation="FKPNQEQNLFPPRLLPQDPTTVHYEGVLNPEVFPFLTYFKNSLI
                     VAFISAGIGVVIAIFGSYSFARLKFTGRGAVQRGVLLVYMFGGVLLVIPLFQMITKLG
                     LFDTRTSLIVTYIVQTLPVSLYMLGNYFRTIPEEIEEAAIIDGCSRLEVIWRIVLPLS
                     APAIVSVFIYAFMIAWNEYLFASVFINSEALKTLPLGLNQLFFTQHYIWGRMMAASVL
                     TAIPVIILFLSVERFITGGLTFGGVKG"
BASE COUNT         4102 a         2560 c         1802 g         5010 t
ORIGIN      
        1 tttttattgg ttgcccgact tgaagttaag gttacaatta catcatcttc tgtctggtaa
       61 ttagcaactg caaagtccat gtgtttttca gaagctacgc ttctagcaaa agcatttaca
      121 tttacaatct cagaatctat tagtgaactt accacatcaa tatcatggat catcaagtcc
      181 tgaataacat cagtgtcatc tattcttggg tcatatggac ccatcctctg tacatcaatc
      241 gcaactattt tttcaccctt taacatctcg ggtaaagctt taatggctgg attaaatctt
      301 tctatatggc caacctgtac tattaatttt ttatcttttg ctctttttaa tatttcattt
      361 gcttcatcaa tcttattagt tattggtttt tcaatcaaga tatgtttacc agcatcaatc
      421 gcctggagag caatatcata atgggtagta gtaggagtag ctatattaac tgcttcaact
      481 tcttgtaata aatcctctac cgccgaaaat gacttaactc cataactttt tgccatttcg
      541 tgggcacgtt tttgatcaat atcatatacg cctactagat cacaaacatg ttttaaagaa
      601 gcataggaac ggacatggtt tttgcccatg cttcctactc caattacccc aacttttact
      661 ttatccatct caaatatcct ccctgtttta tctacaaata cctctatttg aatttttaag
      721 aaagtttata aaatctttta cttccataaa atttttactg actttttcta atttttcaat
      781 agcttgacta atatttagat tcgactcata aagaatatgg tatgattttc ttatctcatc
      841 cctgacatca gggtcaatat ttttttcatt tagaccaact tcattaatac caactacttt
      901 agctgggtga ccatttacaa ttatatatgg tggtaaatct tttatcaact ttgaatggcc
      961 ttcaatccgg cttaaattac ctattttaac aaattgatgc actcctacca tctcttctaa
     1021 ataggcatta ttatatattg taacatggcc ggctagatta acataatttt caagtgtaat
     1081 attattaccc agactacagt catgtgctac gtgggaataa accttaaatg tattgttatc
     1141 acctattttg gtctcccccc gaccttcagt tgttcctcta cgtatcgcta cataatcacc
     1201 gatttgatta ttatctccga taatcacata tgtcttactg ccgtcataac cgatattttg
     1261 agggggaaaa ccaattgtaa catagttatc gattttattg ttttcaccaa tcttggtcca
     1321 cccctgaata tgacagttag caccaatctt agtgttatcc ccaatttcga cttcttcttc
     1381 aatgattgta tatggtccaa tctctatatt ttctcctaac tctactgtat cagatattat
     1441 agctgtatca tcaattttgt tattttttaa caaaattttt tcctcctcaa gagcattaat
     1501 ttattactct aacttgtatt ttatcaaaaa gaagagtatt tttacagaaa taagtgaaac
     1561 tttttcagct agaggtcata actgaagtcg attgaatact tttgcactac tctatcattg
     1621 gaatctgtac tatattgata ctctttattt tttaattgat agcctaattc tatctcaaac
     1681 ttatcactaa tatcccgctg ccaatttatt ttatatgtat ttatcagata attcttacgg
     1741 tattcattta cataagaata ttcttttttc tttaaatcga gcgaatactg gtttttctca
     1801 ttgactgtgt gtctaaactg ataaccaaat gaataggaag tatatgaatt atctctgtta
     1861 tttatatacc tcttatcctt taatttaaac ttgaacttat ttctgaatat ttcattaaac
     1921 tcctgagtat aattgccggt taagtccagg gaacgattat cataagtgct ttcttcgtca
     1981 tacaattttt cataatactt tactttaaag tagtagtaat tcggataatc caacttcttt
     2041 ttcaatttta accagaaatt atcataacta tagtagtcta ccacattctc acttaataga
     2101 tcttgattag aaagataggt attttcgcct ctttctaact taccgctcaa ttcaaaatca
     2161 gctgctgctg gattagtaaa gcatagaact agtaaaataa atattatagt tgatttctgc
     2221 aatataccac acctctatat tatatttaat tgataaaact aaattcaaaa cctatagcct
     2281 tgtctatatg attgtaattt atttgatagc tgtaacagtc atattcgtgt gttactccta
     2341 ttttttgggt atcaaattct tgatctggaa ttgaataagc taaatctaaa tttatcttcc
     2401 agtgttccaa atttttaggt aaatataaat caagttctgt atttgaggtt attactttat
     2461 ccttatctaa ttcatcaata ctaaaaagag gagttccctc ttgatccttg atactgtatc
     2521 ccagtctttg atctaaattc agatttttag aaattctttt atccaaattg aaatatatgg
     2581 ctgtttcata gttagttaat ttttcatcgg cttttggata attaacatgc ttaattttct
     2641 gcttaaaacc tatattgaag ttttttgtaa tcttaatatt tttgttatag tccagttcat
     2701 agtctgtttt atattctttt attgcacctt cctttactag accatatcct atacttagat
     2761 ccaaatcttt gttaaaagaa tatcctatat taccataggg taaataatcg gtattataac
     2821 cttttaaata ttttatcatc cagcccaatt tatttaaatg ttgacttatt gaatattttt
     2881 gcttttgtaa tttttcattg gtaaattcat gataagtttc agtacgtaag ttcttaatag
     2941 gtgtataagt tgtacttacc ttttcaattc tatcttcatt tcggaagtca tatccaaaat
     3001 ccgcattaat gttatatttc tttctggtat aatccacagc aaagtttaac gaagtttgtc
     3061 tttgttcttc cgggatgggt ttaagctttt catagtctct agcatatacc ctataatcta
     3121 tgttttcatt aattttatgg tcaaaaccaa gtttaatctc tctttcttca ttgacaatct
     3181 catcattatc taaatacctc tgctgctgaa aatcaaagaa tatgtattta tcactatcca
     3241 atatatattt atggttccag tcaaaattga tattttcctt gtcttctctg atataataaa
     3301 gatcaccata attatttgtt gaaaactcat agggatatac taattctata tatagaccct
     3361 gttctaaatt atatcctaat tctgggactg gaaaggaatt atctacagta gtttttcctt
     3421 cttcatcagt cgtaacattg actacataac ggggtaaagt aattagtcga ttgccactcc
     3481 accaaaaggt aatattttta cctacaattt tatcattagg ataatactga atgttctttg
     3541 cagtataatg gtaatgtggt tcatccatta tacaggtcgt gtaaaaggca tctttaacaa
     3601 ttatattggg attttgatcc ttcaaataat ctacatcttc cccctcaaat tgaaatttat
     3661 ctatatctaa agaggcctgt gaaagattgg caacaccttt ttcataatca agagttaatg
     3721 atttaccctt tattatccgt ccctcatatt tcaatatgag gggctttcca acagctgtca
     3781 tgatctgatc agcatattta aatactattt ttttcgcatt tatttctacc tgggggttga
     3841 taaatttagc gttaccaaca gcatttattt cttctttatg ggtatttata tatatattat
     3901 cagcttcaat atttgaaacc tgatcatcta tatttccaat ttcaagattg tcatcctgag
     3961 ctagaagagg tgctgtaaac aaaaaaataa agcatattaa aaagatcgat atgtatttta
     4021 tatacttttt catctgcttc ctcctctatt agccttttta tgcccataaa caagacctat
     4081 ttagccaggc taaataggtc tcaatagtaa tcatctttta tttgatttat tcaatgttta
     4141 acccgtaaac attgttatta ttaacagtct gcacataaat ttctgaccct ttcggtagtt
     4201 cttcttcttt acctttcaca aaataaccgg cgataagtcc aactggtcct aaaacggccg
     4261 cacctaaaat gctggctcct aaagccaact gttgggattc attcatcatt tgggcttctt
     4321 catctaagtc gactcttact tctgttccat caatggccat cacttttgaa aaatttaaat
     4381 ctattttgcc ttcttttccc aaattacctg atttttctac ttgggatatt tccattatac
     4441 cggtactacc agccggaata gcaagttttg aatctatgta gacatcttcg gccacttcaa
     4501 atctaactct atcgcctgat ttagaagaaa ctgaacttac tttatcaaat gttttaatct
     4561 ttattaaggt gttttttgga attttgattt gtttagctgg aacagtttca tcaggaaaag
     4621 ttactttggt taaataatta attctttcaa ttaaaggacc atctttagat tcaccaaata
     4681 ccattttttc taatctattt aatttattga taatagtatc ttgggtaata tcagtggtta
     4741 aagaccattc aagtccgttt agatcaaata atagggatgg tgaatcatta ttgtttaata
     4801 cataattata tatattatta gctctttcaa ttaagctacc ttcctttttt tctttatata
     4861 gggtgtcttc taatctttca actctttcta agatagattt atcacttttt tgaccatata
     4921 atatgatttc aactgcactt agtttttcta ccggtactaa ttgtgcagtt gcagttagta
     4981 cattaaaatt aaataaagaa ataatgatca aaaaaataat aatatttctt tttttattca
     5041 tgtatagacc cccaaaggtt aattaaaatt taatctgaaa actagcttct acatttgttt
     5101 cttcaactga ttctgaattg ttagaattat tattggttcc atcctctgtg tcatctgtat
     5161 ttgaagttgg ttcgatatta ttattggcat ccaagagttc tttatttatt accctaaatc
     5221 caagagaaaa actagaatct tcatttaaat taaaatctac tccaaagtcc caggactgtt
     5281 cttgttttaa ttgatctttg atagtctttg gatcaaggag tttatacgaa gcgctaaact
     5341 tagctaaatc ctcatactga taacttaaac ctgtttctct accctgtgtt tctaattcat
     5401 caataatact atattctgct gcaattatat tatcattagt attatattct aatccaaata
     5461 tagtggattc ccctgattgt tctaataaaa tatcgttata tacataatca gcaaatattc
     5521 taatattatc agtagtttga taactaattc caagacgggt atcaatatta ttttcatctc
     5581 tcaataattg actgttttct tctaaattca tagtatctat attattaagt ccaacatcat
     5641 attcttgagt attttgtagt tcatattcag cacttaaagc tgttttatca tctaaattat
     5701 aattaacctg tattgcagtc ttattctcga caatctcatt tttatcttct tgatcataac
     5761 caaagttaat atttaatcta gaagaagggt caataatcag gttctcttga ggttctgtgt
     5821 tttcaatttc ttcattacta aatactatat caagtaaatt attttgattg ctcccatatc
     5881 tattagctaa ttcctttaac ttattttgat actgggcacc aaaatcttct aataccatat
     5941 catcttcagg aaggcgtaag atatagtcat ttatatcaat agaattataa tcaggtgaat
     6001 aaaatataga gttagacaaa ttatcattaa cattttgctc tacttcttga gcaaagatat
     6061 ttaaacttac agtaaacatc aaaactaata caaaatataa gattatcttt ctattcatat
     6121 aaacacgcct cccttctttt taatttctat atgttcatta ttattataac aaaaacaaag
     6181 caatttttct atagtttgtt aaatttaagt aaagaaaatt cctaatactt tttgaaaatt
     6241 aataaatatt atatgtagaa aaagccccgg caaaaccggg gctcttattt ttacataatg
     6301 gtattttgac tagctacaag attcatttag aagctaatac ttacacctgc agtagcttct
     6361 tgtgctgtcc aatcatctgc accatcatct gcgtcatagt ccatttgcat tagcttataa
     6421 ctagctgtta agcttgcatt ttcagtaaat gtgtaatcta cgccaacagc atttgtcata
     6481 tagttaccac tatatagatc ggaagcttca tctttagctt cttgagtagt gtaacccaag
     6541 tcataagtta atgaagtctt ttcattaagt gtataagcta atccagcttt catactagtt
     6601 gtagttgcat catattcttc aaatccaggt gtatcagttg catctaatac attgaagtct
     6661 tcatctggat ttgcagtttc ttcagcatct acaaattctt taccagtaac catcttatag
     6721 tctacagaag ctgaagctgt tagaccagta attactggat aatctgttaa gctgacacca
     6781 acagaagtag tagtcttttc agcatcttca taattactta gaccactaac gtctgtgtaa
     6841 gaaccagtta cacctacata atctgtaaag tcatatgatc ctttgagctc tatagtattt
     6901 tcgttaactg ctttatattc ttctccatcg tcgtcttcag taggtattac atcttcaaca
     6961 ggatccatta ctttataagc tgcacttact tcagcatttt ctactggtac aaaagtagct
     7021 cctacagaat acttagttgt atctccatca taatcagttt cttcaccagt aggctctgta
     7081 aattgtttaa aggaaccttc aacagtcaga ttttccataa ctgccatagt agctccagct
     7141 gaaagtctgg tatcattttc gtcatagcca tcatagtcta ctacatcata gccaaagttc
     7201 aagtcaagac cagaaactaa ggcattttct gcagtaccat catgtgctac tgatacgcca
     7261 tagccagaca ttttttcttg tgctaaacca gcttcgtatt tattataacc ggtatcttca
     7321 tcatcaaata cagataggct tccatataga ggtgagaagt tttctcctct atttttgtaa
     7381 cttgcgccag ctgtgaatgc gtcaaccaat tgagttgaag catttactct aaatagatat
     7441 ccaaattcat cagttactgc atctgtactt acagctacgt caccagcaaa gtcaaatcca
     7501 gaaattttag ctgtagcttc agcgccaaca actaaatctt catattcatc atctgctgat
     7561 tcttcaaaat cagggcttaa gcgtcctgct actgtagttt taacatctgc catatctaat
     7621 acaaatgttt tttgagcacc tgcaactaca taatcagcag tatcagaagt agcatctcca
     7681 ttgaagtata gttcatatac gtcatcttca tcagcatctg cccaatcata atcttcccaa
     7741 gaaacattca gctcttcatc gtcttcagaa tcattggtta atgggtttac tgagtcatca
     7801 acttctactt catcaatacc aacaaatgca tttactccat atggtttata taagaaactt
     7861 gcagcattaa atttctctga gcttacagcg aagtcagaat aactaacttc atttttctca
     7921 ttataaacca tttttaattc atcattttct aattctaaat cagctgattt aaatgtagct
     7981 tcgtcagttg aatctaattt ttctaatgta acaccaactt ggccttcaaa accagcatag
     8041 ttaatatcag catttagatt taaggtgtgg gttaattcag ctatttcttc aaattccata
     8101 gcatctgcat cgccgtcatc ccacatagtt aatgcatcta cagcatcagc agtatcttct
     8161 tgaattctaa aatcagcgag agtataagaa gtacttacag tactccattc tgtattactt
     8221 tctacttctg cagcagtaat ttcgccacct ggaattgtac cagtatagct acccacttca
     8281 gtaggttgac ctactaatac ataattgaac tcagctactg ctaattcaac tagagtactc
     8341 tcagttactt catcttcttc taaagtatca tcaaaaacat ggttataata ctcagcaacc
     8401 acttcgtaat tatttgcagg atcagaatct atttcaccat tcatctctct aattcttgtt
     8461 tcaatgatag cagctacatc agaaacataa gcttcaaatt catcatcgtc taaggctaag
     8521 tcgccatcag catcataagc tgacaatgga gcatcttcga tataataatg attgaagcca
     8581 gaaccagtat caattacttc accatctata acatctttat ggttaaatgt aactgaatat
     8641 gatccgccaa atgtaactgg tggagttgct tcttcgagag ctgtaactct ttctctaact
     8701 gattctaagt tatcttcaag aagacctaag tcgtctctta aagaatcata atcgcttctt
     8761 agaactttaa gctcagcttg atattcaaaa gttaaagctt cgattaaatt aactacttga
     8821 tcagcttgct gatcagtaag ttctgttggt ggttctggag cttctggcat attcttttcc
     8881 attagagctt taacgatagc tgtaacgtct tgagcttgct ctgtagttaa accagagtct
     8941 tttttctcca tggactctac tttgtccatt aactcttgac gctgctctgc catattgtcc
     9001 ataactctac taacgatcat agcgatttcg tatcttgtta agtttctttg tcctttgtaa
     9061 gttccatctg gataaccttc aattactcca gctgcaacta attcattaat tgcttcataa
     9121 gcccagtgat tagatggtac atctgggaaa gaagcaccta ttgctggcgt tgctaaagca
     9181 acaactagca ataaagcaat cattactgta aattttctca tgtttataat tcccccttaa
     9241 ggattgtttt tagttctatt attcattgac aatccttggg actcgaacat atgagtatcc
     9301 ttgtttcctc ataattcttg tctccagttg tcaatgaacg tgtgttagaa cctcattatc
     9361 tcttcgtggc cgcgcggtct cggggggcac ctcctttctc ccgcagttta agccggagat
     9421 aataagactt ttgacgaaaa ttaattcatc actattaaat attcaatacc gagcaaaata
     9481 ttcctgcaaa ataatggata tttttttctg taaatgtcta aaaacaccta ctaactcagc
     9541 atttgaagta attatatcag tgttcttaaa atatttcaag ctaattattc attaaacatg
     9601 ttaatttttt attaaattat cttatccatt taaaatcata tggttttaaa tctataatat
     9661 tttccatttt attattttta agtatatctt caaattcctc cctatttttt aggatttttt
     9721 taagatttat tccttcaatc ttttccttac ttatattatg aagggctaat agttgttccc
     9781 cagtttctct attctctctc agtaatgcaa atattctttc atctatgtcc aatactttct
     9841 gatcagcatt aggatgtaaa gcagactgtt cttttcttat cgatataatt tctgttaagt
     9901 tttcatatac ctggtgcctg attgtgcctt cttcatttat agacgtttct aattcttcta
     9961 aactcagttt ttctctattg atagttcttt tctctccggt cagcttaacc ccttctatat
    10021 aatttcttga acctaataga ctgtgtatat aaatggctgg aatacctcta aaggttaaaa
    10081 gtatagaatg ggcattaata aacttttgga tctttaaatc cataggttct tcctcctgaa
    10141 ctaaggcatc aaaatattta atatttagtt cataaggact tttggtacca tcattattgg
    10201 tcttatagga aacaaaaccg ccattttcct tggttctggt ggctaaacta tcaatctctt
    10261 catttgttag gataccttct accggtctta cgccaatccc atcatgagaa gcaaggaagt
    10321 taaagtaata tttgcctagg ccaaattcct ttaaatcttg tgcccagtca attaattttt
    10381 gggcatctcc tgtatgaaaa gtatgtaaaa ctaaaggagg taatgtaaac tgataaacca
    10441 tatcagcttc tctttcttct tccccgaaat aactaatgtt ttcctcgtaa gggacattgg
    10501 tctctgtaat aattaaaact tctggggcta cttcatctaa gatacacctg aataatttta
    10561 ttacttcatg ggtttctttt aaatgaatag aactagtacc gatctttttc cagagatagg
    10621 ctatagcatc caatctaagg atactggccc cttttttgac ataaaataag agaatatcta
    10681 tgacatttaa aagtagatcc tcattcttgt aatttaaatc tatctgatca ggactaaagg
    10741 tagtccagac gtgttttttt cctctattgg tctcaacttc agttaacaaa ggctttgctc
    10801 tcggtctggt caccttggat acatccacat caggatctac ttctataaag taatctagat
    10861 attcaggatg tccctcttta tacttttgaa accattcact atgactggaa atatggttaa
    10921 taaccgcatc aaacatcagg ttatagtctt cgctgatttc ctcaatatcg gtccagtccc
    10981 ccatttcttc attaacctct ttataatcaa taacagcaaa accatcgtca gaggaataag
    11041 gaaaaaaagg taagatatgt acaatattaa tatagccttt tacatactta tttagaaatc
    11101 tatgtaatac atctaaagta tcatcaccat ctttaacctg atctccataa gtaattaaaa
    11161 ctacatcttc ttctgttaat atatccttat tatatacttt ttctgaaata gctggatttt
    11221 tggctttaaa ttctttcata cgctcataaa gacgagcaaa aacactttct cccttttctt
    11281 ctccataaag agcaattaat ttacccttta tcttttcata tatctcactt ttattttcta
    11341 aagacaagtt atcacctcaa aactaaatgt attattaaca atattatatg aaattagata
    11401 ataatttttc taatatttcc ttcagtcttt ctttagaaaa gttacggcgg gctattacaa
    11461 agttataatt aacagcttcc tggcggtact cattatttgt taaatactta taggcttctt
    11521 ctaaaggctc tttatatctt ttctcttcga tactaactag accgttagtt ttactctcat
    11581 atccactgcc taaagaagct acaattggat tatatttctg gatatctgtt ttaaaaaccg
    11641 gatattcata gatcataatt ggttttttag aaaagaaacc ttccaatagt tgattacccc
    11701 aaccttcttt aatacttgga tatgttatca tatcacagtt tgcatagaaa tcccagaggg
    11761 aatatctgtt ttcttttaat agttgatgcg cccagataat tctaacacct ttttcattag
    11821 ctaatttctt taacttactt atataagatt cattatcttc ttctaggccg ggcatgacca
    11881 aaactatttc attttctttt ctaaactgtc tattatcata taaattatta ttaattaatt
    11941 tttctttgta ttgtctttta aacttactta caaaatttat cgccaattca attgcttttc
    12001 tttccgcaat acgggtagcc tgtaaaaaaa taatatcatt ttcttttaaa ccgagttctc
    12061 tttttaaatc tttattatcc tcattgattt tccagtggtc ctgtttaaaa tcaaatacat
    12121 ttggtacaac ttctgaccag atatcctttt tgttcattaa tttcttttgg gctattctat
    12181 ttattacaac atgtcttatt gaagctagtt tgggtggaaa gtacttctct aacattaact
    12241 caactatctt ataattcgga tcctgataaa gttctctctc ccaatagaaa tcatggtggt
    12301 gggcaatggc agcaagctca agctcttcta tagcttgagc taaaccaaaa cccaccgcta
    12361 tattccaacc aagagaaaaa acattattgg gaatcaataa atcaatatct ctttttttaa
    12421 ttattttttt aaattttgtt ttaaactgat tagcataatt ataaatatct tctttgatct
    12481 gctcttctgt tttagaagga ttctcttttt taaatatttc ttcaacaagt tttagatttc
    12541 tctcatttct atactctaat tccggaaggt atacttcctt ttcatctttt gagcccgtga
    12601 tatatattac ctgatgtcct aaatcttcta atacatctct ccacttatcc atctcaagag
    12661 aaactccatc ggtttgtttt gttctaaaat gtatcattgc tatcttcatt attgcaaccc
    12721 cttatcttaa atattattat cctttaactc caccaaaggt gagtccgcct gtaataaatc
    12781 tctcaactga gaggaataag ataattacag ggatagctgt taagacagaa gctgccatca
    12841 tcctacccca gatatagtgt tgggtaaaga aaagctgatt taaacctaat ggcagagtct
    12901 tgagtgcttc actgttgata aagacagatg caaataaata ttcattccag gctatcatga
    12961 aagcatagat gaaaacggag acgatagctg gagcagagag cggtaaaact atccgccaga
    13021 ttacttctaa cctggaacat ccatctataa ttgcagcttc ttctatttct tccggaatgg
    13081 ttctaaagta attacctaac atatagagcg aaaccggcag ggtctggact atatatgtta
    13141 caataagtga ggtacgcgtg tcaaaaagac ctaatttagt tatcatctgg aataaaggaa
    13201 taactaatag gacgccgccg aacatgtaaa ctaaaagcac acctctctgc actgctcccc
    13261 ggccggtaaa tttaagccgg gcaaagctgt acgaaccgaa gattgcgatt actaccccaa
    13321 ttccagctga aataaaggct acaatcaaac tgtttttaaa ataggtaaga aagggaaata
    13381 cttctggatt caatacacct tcataatgaa cagttgttgg atcctgaggc aacaatctcg
    13441 gtggaaatag attctgctcc tgattaggtt taaa
//
LOCUS       DATGCJ010000250         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_173323, whole genome shotgun sequence.
ACCESSION   DATGCJ010000250 DATGCJ010000000
VERSION     DATGCJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173323"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VKN64_11070"
     CDS             <1..276
                     /locus_tag="VKN64_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HMA60818.1"
                     /translation="DFPPCVEEDPVYAPSGVTNRQVIIDYIQAKGTVNPQPTYNWSIK
                     PFEPAGTITFRSHPEAMGYINDMNIEGIEKLRTDENGMGVYKLDVTE"
     gene            341..541
                     /locus_tag="VKN64_11075"
     CDS             341..541
                     /locus_tag="VKN64_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60819.1"
                     /translation="MKIKVNGELVEIFKGAQVKDVIRSYSEEEYKKVQKGEKIIEDPD
                     GNMVMPNGELMGNEEFFLVDKK"
     gene            574..2712
                     /locus_tag="VKN64_11080"
     CDS             574..2712
                     /locus_tag="VKN64_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMA60820.1"
                     /translation="MSAYLKKRNLIISLFVVAAFIGAMLFAPQMTQAADNTKIVILHT
                     NDEHGSIENFSKIAALKKQYEEEYDDVILVSAGDPSSGNPVVDEYVIDGENLRGKPMF
                     DLMNETGYQALTLGNHEFDYGQERLKANMELANFPMILANLEVDTNEADMPQPEPYTM
                     IESANGIKLSMLGLIQVQKSGYPSTLPKNLYGLKFLDPLKTLKEYTHLKDESDAFIVL
                     SHVGHNWDQEAAEAVDGIDAIIGGHSHTVVQDPPIVNDTLVTQAGGDLAYLGKVVLTF
                     DENKNLVDKTGELIKLENVEKKDKAVQEKIASYQADVEKIFSRELAYIDNSISGNSNL
                     GALMTDAMVNSEEIADLGHEVDFAFQNSGGIRVGSMGPGTLTVGDIFKLEPFGNNIII
                     YEMTTQQIKDMLATNYKRDNSIDLRPGGLKYEIVVNQVGDVKRVELTDMAGNMIEEGK
                     TFTVAHNSYVASSYNFETETEGENTYLRVNDTIIHYLEDVIKPSKVQSYYAAQDLNRT
                     SVSIAKGGSGDKVAYTNVEISTVGKAQGSVSAGNLFADAAKEVLGVDVAAFPSDQLAT
                     GATYPANSDVFNKSLDMLYASFGYDNNVTIATVNGRDLEKMLLSQARYYGEGPVMTHV
                     SKGTEYTVEMENGEIKDIKVYLNGERVSPDKEYTFAINSYVYKYYADAADPISSKTTN
                     ETEKEILVEYLKDKGTITEDVLEERITIEK"
     gene            2945..4273
                     /locus_tag="VKN64_11085"
     CDS             2945..4273
                     /locus_tag="VKN64_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMA60821.1"
                     /translation="MKKNSKLVFSILLLFSLTLVLTSCQFPLYFDKYNASGTVTDTNG
                     NPIEKAVIKFDNGYDPVYTNSSGKWVKSGLGGTVTISVHKTGYSFSPSEYTVTDDETS
                     LNFVTDNVKTTPYNVSGKITDNIGNKIDNVIIRFSNGAEPVETEEDGTWNKDNLKGEV
                     TVTPEKNGFSFDPSSRTVNGKNSYVNFTAIEATDDIYNISGKIEDSLGNGIGDVILRF
                     SGSYESIKTDSDGTYTKEGLKGEVTVTPEKVNYTFDPTERTVNNSDKFINFIGKTSYS
                     LNGSVTLPNSTKGIPNVTINFNSLDSDNTYTSVTTDSEGNWTKSNLKGKIKIIPVLEG
                     WSISPTEEFVSKENENESIDFTASTTSDYEFYKASGKVIDGTASGVAAVRIDFERDGN
                     IIWTTFTDQSGSFTVGEYLYGTVEVIPQGSYEPTSVTITKETTGIQFKTN"
     gene            4457..>5226
                     /locus_tag="VKN64_11090"
     CDS             4457..>5226
                     /locus_tag="VKN64_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMA60822.1"
                     /translation="MKNSKIYYLLIFLILLTTILSGCSTDSSLNYFNIQGTITTESLD
                     KPIEGAIVSIGNKSDSTDSAGNYLIENLKKGKYTWSIESQKYSDYSQEIIVDNNLNIN
                     KQLSLDTVNATITGTVQVYNYNSSSSEDYELQNMTTSSNNIIEPLNLTSEASTPEFKA
                     QQIIINFEENITLNEIDEFINNNNLSKIRKLKLEDNNIYLFQLPDNKAVLEMVDTYNQ
                     HKLITWAEPNYIMSLSAIPNDSSLYNEQWGNRKVNLEPA"
BASE COUNT         1978 a          754 c          930 g         1564 t
ORIGIN      
        1 gacttcccac catgtgtaga agaagatcct gtctatgctc ctagtggagt taccaacaga
       61 caggtaatta ttgactacat ccaggctaaa ggtacagtta atccacaacc tacctataac
      121 tggagtatta aaccatttga accagctggc actattacat tccgctccca tccagaagcc
      181 atgggatata tcaatgatat gaacattgaa ggtattgaaa agttaagaac cgatgaaaat
      241 ggcatgggtg tctataagct ggatgtaact gagtaaattt aaataaataa gaggcaggcg
      301 attatctcgt ctgccttttt tcaatatgaa ggtgaaaaag atgaaaataa aagtcaacgg
      361 tgagcttgtt gaaatattta aaggagctca ggtaaaggat gtaatcagaa gttattctga
      421 agaagaatat aaaaaggttc aaaaaggaga aaagatcata gaagacccgg acggcaatat
      481 ggttatgccg aatggtgaac tgatgggaaa tgaagaattc tttttagtag acaagaagta
      541 aaaaacaaat taaatgaggg ggaatttgta tccatgtctg cttatcttaa gaaaagaaat
      601 ttaattatta gcctatttgt ggtggctgcc tttattggag ccatgctctt tgcaccacaa
      661 atgacacaag cagcggataa tactaaaata gtaatcttac acaccaatga tgaacacggt
      721 tctattgaga acttctccaa aatagcagct ttaaaaaaac aatatgaaga ggaatacgat
      781 gatgtaatct tagttagtgc tggtgatcca tccagtggta atccagttgt cgatgaatat
      841 gtaattgatg gtgaaaattt aagaggtaaa ccgatgtttg accttatgaa tgaaaccggt
      901 tatcaggctc ttaccctggg taaccatgaa tttgactatg gacaagaaag attaaaagct
      961 aacatggaat tagctaactt tcctatgatt ttagctaatc tggaagtaga taccaatgaa
     1021 gccgatatgc ctcagcctga accatatacc atgattgaat ctgccaatgg tattaaatta
     1081 tctatgttag gactaattca ggtccaaaaa agcggttatc cttctacctt acccaaaaat
     1141 ctttacggtt taaagttctt agatcctcta aaaacattaa aagaatatac acatcttaaa
     1201 gatgaaagtg atgcttttat cgtcttaagt cacgtaggtc ataactggga ccaagaggca
     1261 gccgaagctg tagatggtat tgatgctata attggcggtc attcccatac tgtagtacag
     1321 gatccaccaa ttgtaaatga tactctagtt actcaggccg gtggtgactt agcatacttg
     1381 ggtaaagtcg tactcacttt tgatgaaaac aagaatcttg tagataagac aggtgaatta
     1441 attaaattag aaaatgtaga gaaaaaagat aaagctgtcc aagaaaaaat agcttcctat
     1501 caagccgatg ttgaaaagat ctttagccgc gaattagctt atatagataa tagtataagt
     1561 ggaaacagta atctaggtgc tcttatgact gatgcaatgg tcaattcaga agaaattgct
     1621 gatctaggcc atgaagtaga ttttgccttc caaaactctg gtggtataag agtaggcagt
     1681 atgggtcctg gcacattaac tgttggagat atctttaagt tagaaccatt tggcaacaat
     1741 atcattattt acgaaatgac cacccaacaa atcaaagata tgctggctac aaactataag
     1801 agagataact ctattgactt aagaccaggt ggcttaaaat atgaaatagt tgttaatcaa
     1861 gtaggagatg ttaaaagagt agaacttacc gatatggccg gcaatatgat tgaagaaggt
     1921 aaaaccttta ccgttgccca taactcttat gtagcaagtt catataactt tgagaccgaa
     1981 actgaaggag aaaacaccta tctaagagtt aatgacacta ttatccatta tttagaagat
     2041 gtaattaaac catctaaagt tcaaagttat tatgctgctc aagatttaaa tagaacatca
     2101 gtctccatag caaaaggtgg atcaggtgac aaagtagcct atacaaatgt tgaaattagt
     2161 actgttggta aggcacaagg ttctgtaagt gccggtaacc tatttgctga tgccgcaaaa
     2221 gaagttcttg gtgtagatgt agctgctttc ccaagtgatc aattagcaac cggtgctact
     2281 tatcccgcta atagtgatgt attcaataaa tctctagata tgctatacgc cagctttgga
     2341 tatgacaaca atgttacaat agcaactgtc aacggccgcg acttagaaaa aatgctctta
     2401 agtcaagcta gatactatgg agaaggacca gtcatgaccc atgtatcaaa aggaacagaa
     2461 tatacagttg aaatggaaaa tggagaaatt aaagatatca aagtatatct aaacggtgaa
     2521 agagtttcac ctgataagga atataccttt gctatcaaca gttatgtcta taagtactat
     2581 gcagatgctg ctgatccaat ttccagtaaa actactaatg aaactgaaaa agaaatctta
     2641 gttgaatatc taaaagataa aggaactatt acagaagatg ttcttgaaga gagaatcaca
     2701 attgaaaaat aaagaaaaaa tataaattaa taacatctta aaggatcctc cctccgggga
     2761 gggttctttt tttctttaat catcatataa attaaacaga ataaacttat atcccaaaat
     2821 tattgaagga aatcttatac ttatattcaa ttagataata cattggtata gtttggtaaa
     2881 aaagttatta tatatctttt ttgcctttaa tatatcattt aatttaaaag gaggaaatat
     2941 gtatatgaaa aaaaatagta agttagtatt ctcaatatta ttgttatttt ctttaacatt
     3001 agtattgact agttgtcagt tcccgttata ttttgataaa tataatgctt cgggaacagt
     3061 aacagatacg aatggcaatc caattgaaaa agcagtaatt aaatttgata acggatatga
     3121 tcctgtttat actaattcaa gtggtaaatg ggttaaatct gggttaggcg gaacagtaac
     3181 aattagtgta cataaaacag gttattcttt ctccccctca gaatatacag taactgatga
     3241 tgaaacaagt ttaaatttcg taactgataa tgtaaaaaca acgccttata atgtaagtgg
     3301 taaaataact gataatattg gtaataaaat agataatgta ataattagat ttagtaatgg
     3361 tgctgaacca gtagaaactg aagaagatgg tacttggaat aaggataatc taaaaggaga
     3421 agtaacagtt acacctgaaa aaaatggatt ctcctttgat ccctctagta gaactgtaaa
     3481 tggtaaaaat agttatgtta attttacagc tattgaagca actgatgata tatataatat
     3541 ctccggaaaa attgaagata gtttaggtaa tggaataggc gatgtgattt taaggttcag
     3601 cggtagttat gaatccataa aaacagattc tgatggaaca tatactaaag aaggtttaaa
     3661 aggggaagta acagttacac ctgaaaaagt taattataca tttgatccta cagaaagaac
     3721 agttaataat tcggataagt ttataaactt tataggaaaa acatcatata gccttaatgg
     3781 aagtgtaact ctacctaata gcacaaaggg aataccaaat gttactatta attttaatag
     3841 tcttgattcc gataatacct atacatctgt caccacagat tcagaaggaa attggacaaa
     3901 gagtaattta aaaggaaaaa ttaaaattat acctgtatta gaaggatgga gtatttctcc
     3961 tacagaagaa tttgtaagta aagaaaatga aaacgaatca attgatttca ctgctagtac
     4021 aacttcagat tatgaatttt ataaagcttc tggtaaagta attgatggta cagcttccgg
     4081 agtcgcagct gtcagaattg attttgaaag agatggaaat ataatatgga ctactttcac
     4141 tgatcagtca ggaagcttta cagttggaga atacttatat ggcactgttg aagtgatacc
     4201 tcaaggttct tatgagccaa cctctgttac cattactaaa gagacaactg gaattcaatt
     4261 taaaactaac taaaaaaata aaagatattt aaataatcaa tgagaggtct ccttaatgga
     4321 gatctctttt cttttaaata gaatcataat attttaaaat ttgattattt atattaaata
     4381 atagcaggta attattagat aatatttaag tattaatata acgcccaaaa tttttatatt
     4441 aatattggag gtagccatga agaattctaa aatttactac ttattgattt ttttaatttt
     4501 attaactaca atattatctg gctgcagtac agacagcagc ttaaattact ttaatatcca
     4561 gggtacaatt actactgaaa gtctagataa gccaatagaa ggagcaatag taagtattgg
     4621 aaacaaatca gattctactg attcagcagg taattattta attgaaaatt taaaaaaagg
     4681 taaatatacc tggtccatag aaagtcagaa atatagtgac tattcacagg aaattattgt
     4741 tgataataat ttaaatataa ataaacaatt atcattagat accgttaatg ccacaattac
     4801 cggtactgtc caagtttata actataatag ctcaagttct gaagattatg aattacaaaa
     4861 tatgaccacc tccagtaata atataattga acctctcaat ttaacttctg aggcaagtac
     4921 tcccgaattt aaagctcagc agatcataat taattttgag gagaatatta ctttaaatga
     4981 aatagatgaa tttattaata ataataattt atctaaaatt agaaagttaa agttagaaga
     5041 taataatatc tatctatttc aattacctga taataaagct gtcctagaaa tggtagatac
     5101 atataatcaa cataaattaa taacctgggc tgaacccaat tatataatga gtctctcagc
     5161 aataccaaat gattcttctt tatataatga acaatggggt aatagaaaag taaatttaga
     5221 acctgc
//
LOCUS       DATGCJ010000251         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_662637, whole genome shotgun sequence.
ACCESSION   DATGCJ010000251 DATGCJ010000000
VERSION     DATGCJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662637"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..132
                     /gene="rrf"
                     /locus_tag="VKN64_11095"
     rRNA            16..132
                     /gene="rrf"
                     /locus_tag="VKN64_11095"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            206..643
                     /locus_tag="VKN64_11100"
     CDS             206..643
                     /locus_tag="VKN64_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMA60823.1"
                     /translation="MGTNKFKVDSYLKEGMKVKVDNGRGHGIIIDEPESFGGSDEGMN
                     PVEITLASLAGCLSITSAFLANKMKIDINDLSIEVEGEIDDKAMSSAEVDSGFKEIRY
                     NLRIDSDADEEKIKRLYQSIEEYCPVSDTLERAIEVKGDYQLT"
     gene            complement(712..1089)
                     /locus_tag="VKN64_11105"
     CDS             complement(712..1089)
                     /locus_tag="VKN64_11105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMA60824.1"
                     /translation="MKRLIILLSILIVVYTKKNDNFCGELYYKKDAKHKRAGLDIKLL
                     PQMQGRGLATESLQLLISYIFKNEPQIEAVWTEPSEENAAARKLYSRCGLKSKERPTD
                     MKPAASYWELTRKDWQKNNSNFR"
     gene            complement(1040..1258)
                     /locus_tag="VKN64_11110"
     CDS             complement(1040..1258)
                     /locus_tag="VKN64_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60825.1"
                     /translation="MAIKGKRIIIKQTIREDLENIKCLWNNVEVMKWVGFPAGIDQNI
                     NDLKEWWEKIQQNEKTHHFVVYTNCCLY"
     gene            1622..>2471
                     /locus_tag="VKN64_11115"
     CDS             1622..>2471
                     /locus_tag="VKN64_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HMA60826.1"
                     /translation="MKKVHRTFIYLILIIFIFSLSITAAPPGMKDVTFKYEPQIEEVG
                     SVYLAGSFNNWATKKLEMTDEDEDGVYEITISLKPGTYEYKFVVNGSMWKKDPQSEDY
                     APDGFGGLNSVINVGQQIKKVGKSGDGEIIKSALWHDQETPAHITRLGNNIIEIRFQT
                     AVNDVEDVRLNYFDEDGYQVEYMNYFTSDEQFDYWNKKIEVKDNEFRYRFVIDDADTR
                     FWFDRAGLAITMADWFRYNKGNYPLFKTPDWVKEAVFYQIFPDRFYNGNKANDPEYIE
                     TYKNKTE"
BASE COUNT          837 a          341 c          444 g          849 t
ORIGIN      
        1 taaaagttta atatttccgg tggtaattgc ggaggggcca cacctgttcc catctcgaac
       61 acagcagtta agtcctccag cgcctatggt actgcagggg taaccctgtg ggagagtagg
      121 tcactgccgg atcttttttt aactacctta taataaaggt agttttttta atacaataaa
      181 aaacactcaa aggaggttat aacttatggg taccaataaa tttaaagtgg attcttattt
      241 aaaagaaggt atgaaagtta aagtagataa tgggagaggt catgggataa ttatagatga
      301 accggaaagc tttgggggtt ctgatgaagg gatgaacccg gtagagataa cattagccag
      361 tttagcaggc tgtctttcca taacttctgc ttttttagca aataagatga agatagatat
      421 taatgatctt tctatagaag tagaaggtga gattgatgat aaagcaatga gttctgctga
      481 ggtagattct ggctttaaag agatcagata taatttaagg atcgattcag atgcggatga
      541 agaaaaaatt aaaagattat atcaaagtat tgaagaatac tgtcctgttt ctgatactct
      601 agagagagct attgaagtta agggtgatta tcagttaact taaattatta aaaggatgga
      661 gagatccatc cttttttgtg taaaaaataa ggtgataatt aatgtagaat attatctgaa
      721 attagaatta tttttttgcc aatcttttcg tgttaattcc caataggagg cagcgggttt
      781 catatcagtt ggtctttctt tacttttaag tccacagcgt gaatatagct ttcttgctgc
      841 cgcgttttct tctgaaggtt cggtccaaac agcctcaatc tgtggttcat ttttaaatat
      901 ataactgatc aatagttgga gtgattcggt agcaaggcct ctgccttgca tttggggtaa
      961 taacttaata tctaagccgg ctctcttatg tttagcatct tttttatagt ataattcccc
     1021 acaaaagtta tcattttttt tagtatagac aacaattagt atagacaaca aaatgatgag
     1081 tcttttcatt ttgttgtatt ttctcccacc actctttcaa atcattaata ttttgatcta
     1141 ttccagctgg gaagccaacc catttcatca cttcgacatt attccaaaga catttaatat
     1201 tttctaaatc ttctctgata gtttgtttta taataattct tttaccttta atagccatta
     1261 aaatcctcct aatttaaaga ataaaaataa aatatattga ttgaattata tgtatctatt
     1321 ctataatttt ttgttcaaac aagacaaact tcttatctct accaaaattt aataagattc
     1381 ctaataataa aagtgaaatt agatatatac ttactttttt aagcattcta ccatcctcct
     1441 ctttaattat cttatattaa gtataattga actaattact tatttgcaag aaaaataata
     1501 agatattgtt taaagataat aataactcat tttaattatt tatttttgca attattaaag
     1561 gaaatgaaat ccctttattt aaatatatat tagttgaata taatttgaga ggagaatttt
     1621 agtgaaaaaa gtacatagaa ctttcatata tctcatttta attattttta tctttagtct
     1681 atcaattaca gccgccccac caggcatgaa ggacgttacc tttaaatatg aaccacagat
     1741 cgaggaggta ggttctgttt atctggccgg ttcttttaat aactgggcaa ccaaaaaatt
     1801 agaaatgacc gatgaagatg aggatggagt ttatgaaatt actatttctt tgaaaccagg
     1861 tacttatgaa tataaatttg ttgtaaatgg ctccatgtgg aaaaaagatc cccaaagtga
     1921 agattatgct cccgatggtt ttggaggctt aaattctgta attaatgttg gtcagcagat
     1981 aaaaaaggtt ggtaaaagtg gtgatggaga aattataaaa agtgctttat ggcatgatca
     2041 ggaaacccca gcacatatta caaggcttgg taataatatc attgagatca gatttcaaac
     2101 ggctgtaaat gatgtagaag atgttaggtt aaattacttt gatgaagatg gatatcaagt
     2161 tgagtacatg aattacttta cttcagacga gcaatttgat tactggaata aaaaaattga
     2221 ggttaaagat aatgaattta ggtaccgttt tgtaatcgat gatgctgata caagattttg
     2281 gtttgataga gcaggacttg caattacgat ggctgattgg tttagatata ataagggtaa
     2341 ttatcctctt tttaaaacac ctgactgggt aaaagaagca gtcttttatc aaattttccc
     2401 ggataggttt tataatggta ataaggcaaa tgaccctgag tatatagaaa cttataaaaa
     2461 taagactgaa c
//
LOCUS       DATGCJ010000252         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_262958, whole genome shotgun sequence.
ACCESSION   DATGCJ010000252 DATGCJ010000000
VERSION     DATGCJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262958"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(577..1491)
                     /locus_tag="VKN64_11120"
     CDS             complement(577..1491)
                     /locus_tag="VKN64_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60827.1"
                     /translation="MSQIITWLLAYIKLQEKIIIYLFVLLTCKNITPKVDIPISKKYR
                     YLQVDELPIIEVFEKLDYNQLLAEYKAKHGKELKPIKRRNPKHKVLHNISCTRCQAPH
                     VYIYDNNGGKGQYLCKICDFPFIKGQVYQNSISFKCPHCGKSLEKIKVRKQFIIHKCK
                     NDFCSFYQYNLAVMTKKERKLFAQNPYAFKLHYIYREFTFDFKPLSKELTVIPKVDIT
                     KIHSSHQVLGLVLTYYTNYGLSSRNTAAIMKDIHGVDISHQTVLNYANAVSKYIKPYV
                     DNYPYELSNSFCGNETYLKVNGKWSGRN"
     gene            1658..1903
                     /locus_tag="VKN64_11125"
     CDS             1658..1903
                     /locus_tag="VKN64_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMA60828.1"
                     /translation="MKSTGIIRKLDDLGRIVIPKQLRDNFNLLTKDSLEIFKEQNKII
                     LTKYEPGCKFCSEIDVSLVYKGYKLCKDCFENIKELK"
     gene            complement(1990..>2560)
                     /locus_tag="VKN64_11130"
     CDS             complement(1990..>2560)
                     /locus_tag="VKN64_11130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60829.1"
                     /translation="YYNNNAEKYYKNTVEVDMSPLYKEFLKHIKENGHILDAGCGSGR
                     DSLYFLKQGYEVTAIDGSEKLAKLSSELIDQEVKCMKFEDINYKEVFDGIWTCASLLH
                     VKRKDIENILNRLTKALKLNGVLYSSFKYGSKEIYRNGRLFNYYDENSFKELINKFKY
                     LEIIKLWKTEDRRKNRENEYWLNILLKKI"
BASE COUNT          865 a          360 c          457 g          878 t
ORIGIN      
        1 acattctatg tgaagaaacg gttaagtgca acatagaagc tgtcggggta ggggttcatg
       61 catccaggga aggattttca gagataacga cgggaaaatc ctactttcta gttagaataa
      121 taataacaaa gacctatctt ctaggataaa tccaaaatag ataggagaat ttaggattga
      181 cgcatgagat tgtagtagtt aagataggag ggtaatgcct tcttaaatgt agttaaatta
      241 cattactgta gaattaatat tctaaagtgc gaaggatttc tgggcgctta ggtaatcttg
      301 tggaaagtgg tactccgaga ccatctgtaa tttcctgaag agataaaaca ttaaaatgca
      361 aactaaatta tttatgagtg tatataggtg tttctattta aacttgcgat agtttaggaa
      421 ggctaaatgt gagaaatcct aaggtcaaaa ccggattcgg gaaaaccgac cgtcttggat
      481 tgtagggggg ctctgggaaa cgtaatcaaa gagagctgga ctgcggtctg gtgggaaatc
      541 gaccagaaaa gccaccggac tctaaagtat acgcgctcag tttctacccg accatttacc
      601 gttaactttt agataggttt catttccaca gaaggaatta gagagttcgt aagggtaatt
      661 atcgacatag ggtttaatgt acttactgac agcattagca tagtttaaga cggtttggtg
      721 tgagatatct acaccgtgaa tatctttcat gatggcagcg gtattacggg aagagagccc
      781 gtagttagta tagtaagtga gtactaaacc gagaacctga tgagaggaat gaatttttgt
      841 tatatcgacc ttaggtataa cagttaactc tttgcttaag ggtttaaagt caaaggtaaa
      901 ttcacgatag atataatgta gtttaaaagc ataaggattt tgagcaaaaa gttttctttc
      961 ctttttagtc atgacagcta ggttatactg ataaaaagag cagaagtcgt ttttacactt
     1021 atgaatgata aactgtttac gcactttaat tttttcgagt gatttcccac agtgtggaca
     1081 tttaaaagag attgaatttt gataaacttg tcctttaata aaagggaaat cacagatctt
     1141 acagaggtac tgtcctttgc cgccgttatt atcgtagata tagacatgag gagcttgaca
     1201 gcgggtacag gagatattat gaagtacttt gtgtttggga tttcgccttt taatgggttt
     1261 aagttcttta ccgtgtttag ctttatattc agctaagagc tggttataat ccaacttttc
     1321 aaaaacctcg atgataggca gttcatcgac ttgaagatac cggtattttt tactgatcgg
     1381 aatatcgact ttgggagtaa tatttttgca ggtcaaaagg acaaaaagat aaatgataat
     1441 tttttcctgt aatttgatat aggcgagtag ccaggtaata atttgagaca taattcttgc
     1501 cacctttctt tagatttttt tgttagataa gtatatttta cccaaaaatc aagggggtgg
     1561 caaggtttta aaccaaagtc cttgttttga cagggttttg gactatattg attagaaaac
     1621 ttttgacaat atgaatttaa gtaaggagag ttttaaaatg aaatctactg gaattattag
     1681 aaagttagat gacctaggta gaattgttat tccaaagcaa ctaagagata attttaattt
     1741 gttaactaaa gactccttag aaatttttaa agaacaaaat aaaataattt taactaaata
     1801 tgaaccggga tgtaaattct gtagcgagat tgatgtgtca ttagtttata aaggctataa
     1861 actatgtaaa gattgttttg aaaatataaa agaattaaaa taattaatac aaaaagagaa
     1921 ctatctttta acaaaaaatc aatagttctc ttttaatatt aattagtata agttcaaata
     1981 agaaatatat tatatttttt ttaataagat atttaaccag tattcgtttt cacgattttt
     2041 tctgcgatct tcggtcttcc ataatttaat tatctccaaa tatttaaact tattaataag
     2101 ttctttaaaa gaattttcat cataataatt aaataaccta ccatttctat aaatttcttt
     2161 actcccatac ttgaaagaag agtataatac tccatttaat tttaatgcct tagttaatct
     2221 atttaagatg ttttcaatgt cttttctttt aacatgtaat aaagaagcac aggtccaaat
     2281 tccatcaaat acttccttat agttaatatc ttcaaatttc atacatttta cctcttgatc
     2341 aattaattca gaacttaatt ttgctaactt ttctgaacca tcaatagcag taacttcata
     2401 tccctgtttt aaaaaatata gactgtctct tcctgatcca catcctgcat ctaagatatg
     2461 cccattttct ttaatatgtt ttaaaaactc tttatataag gggctcatgt caacttctac
     2521 agtattttta taatattttt cagcattatt attatagtat
//
LOCUS       DATGCJ010000253         9331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_40808, whole genome shotgun sequence.
ACCESSION   DATGCJ010000253 DATGCJ010000000
VERSION     DATGCJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40808"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2213)
                     /locus_tag="VKN64_11135"
     CDS             complement(189..2213)
                     /locus_tag="VKN64_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMA60830.1"
                     /translation="MNQKFNFNSITTKFLLPTLLVIIVGMAVIGFIGYNTQKNYVMNS
                     IEEESNLQIEEIKNTIEERENNAQMTEEAIDKYLLMVTESINEIFANTPNFLLQNKIN
                     SLVNSLNIPEIHVTDGNGVIKWSSVDDFIGFDFHSSEQTKPFLEGLENDDFQLSQDPQ
                     LRGTDGSMFKYVGVARKDKKGIVQIGVQPTELQSLLDKINIKRIADNTTYGENGYVYI
                     TDKQGTIISHPDDSLVGTSINEYEWGQQIVNKEEGEIVNTFNEQKLLQNFKPYEDHLI
                     VTALPTSEYQAPLNIFRNKIIITIFLAIIIASVITYLTTRSVVNPLQKAQGFAEKIAD
                     GDLAVEKLESKSKDEIGELSASLDHMRDSLRALIGKVAEVSNNLSASSEELSASGDEV
                     TNSAEHVGNAIEDVASGAEEQTAQVEETSENIKDLIDRLDEAEDKSVNLEELEQEVME
                     NINKGNKSIQQSVNKINQVKDYYKDVADTMDSLGQSSNEIGEIVNLINDIAAQTNLLA
                     LNAAIEAARAGEAGRGFSVVADEIRELAEESANATEQISELINDIQDDVDSAVDKMDK
                     TEEAVDQGVETIETSGEIFGKINDTSQNSNHAIKKLHESIEEMNKNSKQVENAVEQIE
                     EVSKSAASNAQEVAASSEEQIASTEEIVNAAKELAEMANELSDNVNKFNL"
     gene            complement(2256..2627)
                     /locus_tag="VKN64_11140"
     CDS             complement(2256..2627)
                     /locus_tag="VKN64_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60831.1"
                     /translation="MDLYDYYKNYYKLTKQQKENIKNNEINNLPNLIKKKHEIIKQIE
                     ENTDLEDYLNNQDDPKEAFAELKELMEKINALEKENTNSVENKKEKILDKMNDLNQKA
                     KSRKGYLAQNKFEAKFIDKKS"
     gene            complement(2657..3097)
                     /locus_tag="VKN64_11145"
     CDS             complement(2657..3097)
                     /locus_tag="VKN64_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60832.1"
                     /translation="MAEINSIGSYENQLPIKNNIINKNKESNKQNKDNIKKKKDKVLI
                     KSKNNNKQNNSINKSYNIKDGVKRPIKIDNSNNIFFVNYKGENETIKIKPGQYNLTEI
                     SLSIQEKINESFGIGKVNLKMTGSLSDGFIHAEEDKTNKFLIEE"
     gene            complement(3149..3559)
                     /gene="fliS"
                     /locus_tag="VKN64_11150"
     CDS             complement(3149..3559)
                     /gene="fliS"
                     /locus_tag="VKN64_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HMA60833.1"
                     /translation="MSMIAHKQYKQVQVKTANNKKLLIMLYQGCIKFLRIAKKSIKED
                     DFENANEYLKKSQDIINELRYTLDMEKGGEISNNLYQLYSFMYSELVSANVNKDIQHI
                     ETVEEMMLELLETWKEIINNPQQKQGEQNINRSG"
     gene            complement(3608..3802)
                     /locus_tag="VKN64_11155"
     CDS             complement(3608..3802)
                     /locus_tag="VKN64_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HMA60834.1"
                     /translation="MDINSAIQNQIASVKSAINMSTLQTSMNQDGATVNKLIEGMEET
                     SKAVQEASLPHRGNNIDIRA"
     gene            complement(4420..5007)
                     /locus_tag="VKN64_11160"
     CDS             complement(4420..5007)
                     /locus_tag="VKN64_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60835.1"
                     /translation="MSIYINDEKTDLETTGKDKEKILKKIKGNLNDEIIDRVYLDEVE
                     VSLAYFKENNINSDKIEKINFVTKKNEILIKETLILAKKYLTKLENALIQTAQLYENY
                     MIYQGAEKLDKCLSGLEWYTEVTNSIYNLIDKEIISDKGKELLDKLHRANTRAMVALQ
                     KEDYQYFSDIIEVKVVEVLDELDVLNDSILNNELK"
     gene            complement(5218..7029)
                     /gene="fliD"
                     /locus_tag="VKN64_11165"
     CDS             complement(5218..7029)
                     /gene="fliD"
                     /locus_tag="VKN64_11165"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HMA60836.1"
                     /translation="MANLGLDGLASGMGTEEIINQLVQLERKPIINMQQEKMGLEESK
                     NAWRDVNSRLDKLEGKLTQLKLSSTFQSNSTTSSNEDVATATATSDAAAGNYNLNITK
                     MAQSLRIASDKQLDSSSELGLSGDVSINGQTVTIEATDSLNDINNTINETADIGVTSS
                     IIDNTLILESSDTGFDNKISLQDSNNVLSSLGLVSGNKTNLAETATFSASSEHSSGDY
                     PVSSLNDGDTTSWGNGDGWNDDTSSDFSNDWVQMDFGSTKIVDQVKVHTLDTAEHPAY
                     EYGVKDYEIQYWNSDSGSWQTVEEVNGNTEGSVENNFSAVETDKIRIKVNDSNDGKYS
                     RLTEVEAYNTNDQYKNVLQNASDAELNINGVDVTSSSNNIEDAVKGVNFNINSTGSTE
                     INVSQDTKKATSAVQEFVDQYNSVMDFVDTKLDYDPEEDKAGALQGDSTLMRLQMRLR
                     SMVTDRVKEADSYNQLTSVGIEIDRDGVMSFDSGKMTEAIADSPEKVMNLFNAEQDED
                     GFDGVATRMDQYVDQLLQSNTGTIPRKIDFFNTRMDNIDEDIEDQERRLESTRERYRE
                     QFTAMEEAMSQMQQQQSWMQQQLASLNTGGNMISSMM"
     gene            complement(7049..7417)
                     /locus_tag="VKN64_11170"
     CDS             complement(7049..7417)
                     /locus_tag="VKN64_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HMA60837.1"
                     /translation="MVGRVEGIDQSQDAQRINLARREPRTERTENVRGKVKSLKENQL
                     SAEELRDELEDKINDMNDIMETLDEKLSFELHDQTDRIMTRIIDIKTQEVIKEMPPEE
                     MLDLAAKIHEMVGLIIDEEV"
     gene            complement(7509..9089)
                     /locus_tag="VKN64_11175"
     CDS             complement(7509..9089)
                     /locus_tag="VKN64_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HMA60838.1"
                     /translation="MRINTNVAALNSYNQLKHTQNNLSKSLSRLSSGKRINGAADDAA
                     GLAISEKMNAQTRGLAQAQRNAQDGISMIQTAEGALKESHSILQRMRELSVQSANDTN
                     TSADRAEIQKEVDQLANELTRISNNTEFNTQTLLNGAIQEGNSGEAVFQIGANEGQNI
                     NLGVSAMDATSLGVAADVDVFEADTTTGLGNVEMSEVAESGSEITDGDTITLNQTTVA
                     SDTSATITGGAHTVGTDVSGATQADAEITVDGTTYTADLSSWDGTGDEAALITALEGA
                     DDGSGTTLGSVASVTGDGSNLTITANSSGSASSVEVAYTGDDAAVETSLETLTGITTG
                     TSDTGEDAGDYVEVSDGSTTQSVEISDTSATEFSVTDGNFEGISIETSSGETLSDLNG
                     AGNHEFTVSRTTQTSEAATFTAEGELDSEAITYAGIDVSTQANADAAITTIDKALENV
                     SGERSKLGALQNRLDHTINNLNTAQENLTAAECRISDVDMAKEMMNMSKQQILSQAGT
                     AMMAQANQLPQGVLQLLG"
BASE COUNT         2544 a         1829 c         1328 g         3630 t
ORIGIN      
        1 aatatgaatt atttaatttt tttgtaccgc tattataata tttttataaa agttatttat
       61 ctcctcctaa aaatttgaga tttttataaa aaaattaaat tgatagtaaa tattgtaatt
      121 ttagaatata ttaaattaaa agtactaaag gctatcccta aacaggtttt aaggatagcc
      181 ttgctaattt ataggttaaa tttatttaca ttatctgaaa gttcatttgc catttctgct
      241 aattctttag ctgcattgac tatttcttcg gttgaagcaa tctgctcttc gctggaggct
      301 gccacttcct gggcattact ggcagcgctc ttacttactt cttctatctg ctctactgca
      361 ttttcaacct gtttgctgtt cttgttcatt tcttctattg attcgtgtag ctttttaatc
      421 gcatggtttg aattttgaga tgtatcattt atcttgccaa agatctcacc ggaagtttca
      481 attgtttcta caccttgatc aacggcctct tcagttttat ccatcttatc tacggcagaa
      541 tcaacatcat cctggatatc attaattaat tctgatatct gttcagtggc attggccgat
      601 tcttctgcta attctctaat ttcatcggct acaacactga aaccacgtcc ggcttcaccg
      661 gcccgggctg cttcaatggc cgcatttaag gctagtagat tggtctgggc tgcaatatcg
      721 ttgatgagat ttacaatttc tccgatttca ttagaagatt gacctaagga atccatggta
      781 tcagcgacat ctttatagta atcttttacc tgatttattt tattgaccga ctgttggata
      841 gatttattcc ctttattaat attttccata acttcttgtt caagctcttc taagttaact
      901 gacttatctt ccgcttcatc caatctatcg attaaatctt taatattttc agatgtttcc
      961 tcaacctggg cggtctgctc ttctgccccg gaagccacat cttcaatagc attcccaaca
     1021 tgttcagccg aatttgttac ttcatcaccg gaagcagaaa gttcttcact tgaagctgaa
     1081 agattatttg atacttctgc tactttaccg attaatgctc tcaaactgtc acgcatatga
     1141 tcaagtgaag cggaaagttc tccgatctca tctttacttt tactctccag tttttcaacg
     1201 gctaaatcac catctgcaat tttttctgca aatccttgtg ctttctgcag aggatttact
     1261 acactcctgg tagttaagta agttattaca gaagcaatta tgattgctaa aaatattgtt
     1321 atgattatct tatttctaaa aatatttaaa ggagcttgat attcactagt cggtagggca
     1381 gttactatta aatgatcttc atatggttta aaattctgta gtaatttctg ttcattgaag
     1441 gtattgacaa tttcaccttc ctctttattt actatttgct gtccccattc atattcattg
     1501 atacttgttc ccaccagact atcatccgga tggctgatga ttgttccctg tttatcagtt
     1561 atatagacgt agccattttc accataagtt gtattatctg caattctctt gatatttatc
     1621 ttatccaaaa gactttgtaa ttcggtgggt tgaactccaa tctgcacaat acctttttta
     1681 tctttgcggg ctactcctac gtatttgaac atcgagccat ctgttcctct taactgcggg
     1741 tcttgagaaa gctgaaaatc atcattttct aaaccctcca aaaatggttt ggtctgttca
     1801 ctgctgtgaa aatcaaaacc aataaaatca tcaacactgg accacttaat aactccattt
     1861 ccatcagtta cgtgaatttc aggtatattt aaactattaa ccaaactatt gattttattt
     1921 tgcagtaaga agtttggtgt gtttgcaaat atttcattaa ttgattctgt caccataagt
     1981 aaatatttgt cgatagcttc ttctgtcatc tgagcattat tttctctttc ttctatagta
     2041 ttttttattt cttcaatttg cagatttgat tcttcctcta tggaattcat tacataattt
     2101 ttctgtgtat tatatcctat aaatccgatt actgccatcc ccactattat tactaataag
     2161 gttggtaaca gaaatttggt agtaatactg ttaaagttaa acttctgatt caattataac
     2221 aacccctttg gctgattcac tgttaaatta atattttaac tttttttatc tataaattta
     2281 gcttcgaatt tattttgagc aagataaccc ttcctactct tggccttctg gttaaggtca
     2341 ttcatcttat ctagtatttt ttctttttta ttttctaccg aatttgtatt ttctttttct
     2401 agagcattga ttttttccat tagttctttg agctctgcaa atgcttcttt tgggtcatcc
     2461 tgattattta agtaatcttc caaatctgta ttttcttcta tttgcttgat tatttcatgc
     2521 ttctttttaa tcagattagg aagattattt atttcattgt ttttaatatt ttctttttgt
     2581 tgtttcgtta atttataata atttttataa taatcatata agtccatatc tgcacttcct
     2641 taaaaaaata agtgaattat tcttctatga ggaacttatt tgtcttgtct tcctcagcgt
     2701 ggataaaacc gtctgaaagc gaaccggtca tttttaagtt tacttttcct attccaaatg
     2761 attcattgat cttttcctga attgataggg agatctctgt taagttatac tggccgggct
     2821 tgatcttaat ggtctcattt tcacccttgt agtttacaaa aaagatattg ttgctgttat
     2881 ctatttttat aggtctcttt acaccatctt tgatattgta acttttgttt attgaattat
     2941 tttgtttatt attattttta cttttaatta gtactttatc ttttttcttt ttgatattgt
     3001 ccttattttg cttattactt tctttatttt tatttataat attatttttt attggtaact
     3061 gattctcata agatccaata ctattgatct cagccatttt attaccccct agtttagatt
     3121 tgccagggct tttaaattta ttattatatt agccgcttcg attaatattt tgttcccctt
     3181 gtttttgctg tggattatta atgatttctt tccaggtctc cagtaattcc agcatcattt
     3241 cctcgacagt ttcgatgtgc tggatatctt tatttacatt ggcgcttacc agttctgagt
     3301 acataaaact gtataactgg tagaggttat ttgaaatctc tccgcctttt tccatatcaa
     3361 gggtatatct caattcattt attatatcct gtgatttttt taaatattca ttggcatttt
     3421 caaagtcatc ttctttaata gatttcttgg ctattcttaa aaacttgata cagccctggt
     3481 ataacattat aagtagtttt ttattattgg ctgtcttaac ctgtacctgt ttatattgtt
     3541 tatgtgctat cattgacatt atatttcctc cttaaatcat gttcgttgtt atttcaatta
     3601 aaaaacctta agcccttata tcaatgttat tacccctatg tggtaaagaa gcctcctgga
     3661 cagccttact ggtttcttcc atgccttcga ttaacttatt tacagttgcc ccatcctggt
     3721 tcatagaagt ctgcaaggtg gacatattaa tggctgactt cacacttgca atctgatttt
     3781 ggatagcaga atttatatcc attttagatt ctcccttctt ttagtgattt agaaaagata
     3841 agtttctaaa tttatgggct ctaatttatt ctttatatac tttctatcac cttcattatc
     3901 ttataacgaa atatgtttaa attgtattaa attacttgtg agttagttaa gtacttttaa
     3961 ttagcattag gacatattta ctaccttatt ctacatataa ttacctcatt attatttttt
     4021 atctaattaa agccaataaa atcagttaat aaagaagcaa gataaattag acttaaattt
     4081 aactttgtcg aataaattta gcagctgtta aaaattatta caagaggtta tatttaatag
     4141 aaaaatcatc gttatttctt ttttaaaaaa gaaaataccc catatcacag gagtacccgg
     4201 tttttctaaa tatttataat tttatttctc tgaaatttct cttgaattta tatttttcat
     4261 taaaaatgta aaattacagg acttaattag aaaatagtgt atgttattta gaaaggtgtt
     4321 taataacaat tattttttac tgttcagcta atatccttat tgatcaaaaa tttgtcatat
     4381 taaaaaactg tctctagttg gatatttttc ttatacctgt tatttcaatt cattatttag
     4441 aatgctgtca ttaaggacat ctaattcatc aagcacttct accactttaa cttctatgat
     4501 atcagaaaaa tactgatagt cctctttctg taaggccacc atggctcgcg tgtttgccct
     4561 atgtagttta tctaagagtt cttttccttt atctgatatt atctctttat ctattaggtt
     4621 atagatgcta tttgtaactt cagtgtacca ttctagtcca cttagacact tatccagttt
     4681 ttctgctccc tgatagatca tataattttc atataattgg gctgtttgaa ttagggcatt
     4741 ctctaatttt gtcaggtatt ttttagccag gataagagtt tcttttatta agatttcatt
     4801 ctttttggtc acaaaattta ttttttctat cttatctgaa ttaatattgt tttctttaaa
     4861 gtaagcaaga gaaacctcaa cttcatccag gtaaaccctg tcaatgattt catcattaag
     4921 gtttcccttg atctttttca gtatcttttc tttatctttc cctgtagttt ctaaatcagt
     4981 tttttcgtca tttatataaa tgctcatagt aaaaatatcc tttcttttct ttactgttta
     5041 ttttactttt taaccaatca ttagtattag ctataaatct aagagagtaa cattggaggt
     5101 tttctttatt aactgttttt acgaatatcc aacaaggttg ttttgaacta tttagcctct
     5161 caatcaataa tccagtttaa tactttttta aaaaaatatt tataattaat tcaatattta
     5221 catcattgaa cttatcatat taccaccggt gttgagactt gccagctgct gctgcatcca
     5281 gctctgttgc tgctgcatct gggacattgc ttcttccatg gctgtaaact gttctctgta
     5341 acgttctctt gtagattcca accttctctc ctggtcttca atatcttcat caatgttatc
     5401 cattcttgtg ttgaagaaat ctatttttct gggaattgta ccggtattgg actgtagtag
     5461 ttgatctaca tattggtcca tccgggtagc aactccgtca aatccgtctt catcctgttc
     5521 agcattaaag aggttcatta ctttttcggg tgaatctgct atagcttcag tcatcttgcc
     5581 gctatcaaaa ctcataacac catctctgtc tatttctata cctacagagg ttaattgatt
     5641 atagctgtca gcttctttta ctctatctgt aaccatactt ctcaacctca tctgtaagcg
     5701 cataagtgta ctatctccct gcagggcccc agctttgtct tcttctgggt cataatcaag
     5761 ttttgtatcc acaaaatcca taacactatt atactggtca acaaactcct ggactgcaga
     5821 agttgctttc tttgtgtcct gacttacatt aatttcagta gatcccgtac tattaatatt
     5881 aaagttaact ccttttacgg catcttctat attatttgat gaacttgtta catccactcc
     5941 atttatatta agctctgcat cactagcatt ttgaaggacg ttcttatatt gatcattagt
     6001 gttataggct tctacttctg ttaatctaga atacttacca tcattggaat cattaacttt
     6061 tattcttatt ttatcagtct ctactgcaga gaagttgttc tccacagaac cctctgtgtt
     6121 tccattgact tcttctactg tttgccagct accactatca ctattccagt attgaatttc
     6181 atagtcttta accccatact cataagcagg atgttcagct gtatcaaggg tatgaacttt
     6241 cacctggtca actatcttag tagaaccaaa atccatttgt acccagtcat tactaaagtc
     6301 actacttgta tcatcattcc aaccatcacc attaccccag cttgtagtat ctccatcatt
     6361 aagtgatgaa acaggataat caccactact gtgctcacta gaagcactga aagttgccgt
     6421 ttcagccaaa ttagtcttat ttcctgaaac tagccccagg ctagaaagaa cattattact
     6481 gtcctgtagt gaaattttgt tatcaaaacc agtatcactg ctttctagga tgagagtatt
     6541 atcaattata gaagaagtaa ctcctatatc tgctgtttca tttatagtgt tattgatatc
     6601 atttaggcta tcggtagctt caatggttac tgtttgtccg ttaattgaga catccccaga
     6661 aagaccaagt tcactacttg agtctaactg tttatctgaa gctattctca agctttgggc
     6721 catctttgta atatttaaat tgtaatttcc agccgcagca tcacttgtag cagttgctgt
     6781 ggccacatcc tcattactgc tcgtcgtaga attagattga aaagtagaag ataatttcaa
     6841 ttgggttagc ttaccctcta atttatcaag gcgggaatta acatcacgcc aagcattttt
     6901 actttcttcc aggcccattt tttcctgctg catatttatt attggttttc tttctaattg
     6961 aactaattga tttattatct cttcagtacc catacctgaa gcaagaccgt ctaagcctaa
     7021 atttgccatt ataactcacc tcttttcctt atacttcttc gtctattatg agtccgacca
     7081 tctcatgtat tttggcagcc aaatctaaca tttcttccgg cggcatttct tttatcactt
     7141 cctgggtctt tatatcgatt attctggtca ttattctatc tgtttgatca tgaagttcaa
     7201 aagatagctt ttcatctaag gtctccatta tgtcattcat atcatttatc ttatcttcca
     7261 gctcatctct taattcttca gcagagagtt ggttttcttt taaagattta actttacctc
     7321 taacattttc agttctctcg gttcgcggtt ctctccttgc caaattaatc ctttgggcgt
     7381 cctggctctg gtcaatacct tctactcttc ctaccatcct ccctcactcc ttttaaaagt
     7441 agttaagcca gccagcaaat aagccggctg gctttataat tagattgata tattttatta
     7501 gatagacatt aaccgagtaa ttgaagtacg ccctgtggca gttggttagc ctgtgccatc
     7561 attgcagtac cagcctgaga aagaatctgt tgcttactca tgttcatcat ttctttagcc
     7621 atatctacat cagagattct acattcagca gctgttaagt tttcttgagc tgtattcaga
     7681 ttattgattg tatggtctaa tctgttctgt aaagcaccca atttggaacg ttcccctgat
     7741 acattctcaa gagctttgtc aatagtagtg atagctgcat cagcattagc ttgtgtactt
     7801 acatcaattc cagcataggt aattgcctca gaatctaact caccttcagc agtaaatgta
     7861 gcagcttcag atgtctgagt agtacgtgaa actgtaaatt catgattacc agcaccattt
     7921 aaatcagaaa gggtttctcc agatgatgtt tcaatactga taccttcaaa attaccatca
     7981 gttacactaa attctgtagc tgaagtatcg gaaatttcaa cagactgagt tgtactacca
     8041 tcagaaacct ctacataatc accagcatct tcacctgtat cactagtacc tgtagtaatc
     8101 ccagtcaatg tttctaaact ggtttcaact gctgcatcat caccagtata agcaacttca
     8161 acagaagaag cggagccact tgaattagca gtaattgtca agtttgaacc atcacctgtt
     8221 acagaagcaa cactacccaa agtagttcca gatccatcat cagcaccttc taaagctgta
     8281 attaaagcag cttcatctcc agtaccatcc cagcttgata aatcagctgt ataagttgta
     8341 ccatctactg ttatttccgc atctgcttgt gtagcacctg atacatcagt tcctacagta
     8401 tgtgctccac ctgtaattgt agctgaagta tcactagcaa cagtagtctg atttaaagta
     8461 atggtgtctc catcagtaat ttctgaacca ctttcagcaa cttctgacat ttcaacattt
     8521 cctaaacctg tagttgtgtc ggcttcaaat acatcaacat cagcagcaac acctaaagaa
     8581 gtagcatcca ttgcactgac acctagatta atattctgac cttcgtttgc accaatttga
     8641 aaaacagctt caccggaatt cccttcttga atcgcaccat ttaaaagagt ttgagtatta
     8701 aattcagtgt tattagaaat tctagttaat tcatttgcta actgatctac ctctttttgg
     8761 atctctgctc tatctgctga agtgttagta tcgttcgcag attgaacaga aagttctctc
     8821 atcctctgta aaattgagtg agattctttt aaagctcctt ccgctgtctg gatcattgag
     8881 ataccatcct gagcgtttct ttgagcctgt gctaaacctc ttgtctgagc attcattttt
     8941 tcactgattg ccagacctgc agcatcatcc gcagcaccat taattctttt acctgaagac
     9001 aatctcgata aagacttact taaattattc tgtgtgtgtt ttagctgatt ataagagttt
     9061 aaagcagcga cattcgtgtt aattctcatt atttattcct ccttgaattt tgttttatcc
     9121 ggcatccttg ccagatatag ctttttatat ggttatatga acaccgcctt tagcaacact
     9181 tcggccgata gtgatgctgg ttacgggttt tagtaaccat tataggactg ataaattcag
     9241 gcctataatc attactaaat actttattta tcttctaatt atattatcgg cttattctta
     9301 aattagttaa ctaaataaaa atcttgttaa t
//
LOCUS       DATGCJ010000254         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_40861, whole genome shotgun sequence.
ACCESSION   DATGCJ010000254 DATGCJ010000000
VERSION     DATGCJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40861"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..955)
                     /locus_tag="VKN64_11180"
     CDS             complement(80..955)
                     /locus_tag="VKN64_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60839.1"
                     /translation="MEWTDVLRRLFTNKLSVFGLVIVIFFAVVAIFAPWLAPPADPDE
                     PFNIPRSGWTIQPTPPDADNIFGTTEGSYDIYYGMVWGSRTAFRIGFIVVGISTLIGI
                     FVGTVSAYYGGWFDEIIMRVTDIFMSIPFLVAALVLTAILGKGLDKVMIALIVFGWMG
                     TARLMRSQVLEIKAEEFIDAAYALGAGDTRIIFKHVLLNCIFPVLIQACMRMGSMVIT
                     AATLSFLGVGAPQGYADWGQMISFARNWIIGTQGNPLTFWYTLIIPGTAILLFSLSWN
                     LIGDAFRDILDPKLQ"
     gene            complement(964..1935)
                     /locus_tag="VKN64_11185"
     CDS             complement(964..1935)
                     /locus_tag="VKN64_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60840.1"
                     /translation="MLVFGMLMMLGPYQRLATYVTDPTQLKGGDLDLLITKYGLDEPF
                     YMQYGRWANGLLHGDLGWSEAAGAPVVQAITDRFPATLELAILAVIPVIMGGIWLGVF
                     SAIHHNDFWDHATRIFSTVGWSFPTFVFGLIILMIFYGVFGWTPPGRLSNWAIELKNT
                     QEFITYTDLNIIDGLFNGNFDFILDALQHLVAPVFTLAVVWWAFILRITRSSMLETLR
                     KDYIRTARAKGVKENVVINKHARRNALIPVVTVAGPMVLGLLGGIVTVETVFGYRGLG
                     LLAYQTATRLDYAGILGVAMYFGGILVLINLIVDVSYAFIDPRIRLE"
     gene            complement(2113..>2657)
                     /locus_tag="VKN64_11190"
     CDS             complement(2113..>2657)
                     /locus_tag="VKN64_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMA60841.1"
                     /translation="EHFRQAWDGEVWEKGFKLTILYNSGNMTRKTAADIFKSYLEQIN
                     PKFDIDTRAVQWSTFLDESARGTLPLQIGGWLADFPDPHNFVQPFLHSQGYYGGKRGE
                     NYAEWAEENVDPLIDQGIGELDSQKRIELYKELQSISHDQAIDVWLDQPFSSRIQRDW
                     VKGWYFNPMRPGQYFYILDK"
BASE COUNT          872 a          546 c          384 g          855 t
ORIGIN      
        1 tatgaaaata tgtttgtaaa tcattaacat ctataagctt ttctttactc aaatctatca
       61 cctactttac ttattttatt tattgaagtt ttggatcaag gatatctctg aatgcatctc
      121 caattaagtt ccaggataaa ctaaataata aaattgctgt accaggaata attaaagtat
      181 accaaaatgt taatggatta ccctgagtac ctataatcca gttacgagca aatgaaatca
      241 tttggcccca atctgcatat ccctgaggtg ctccaacacc aaggaaactt aatgttgctg
      301 cagtgataac cattgaaccc attctcatac atgcttgaat taaaactggg aatatacagt
      361 ttaataatac atgtttaaat ataattctag tatctcctgc tcctagagca taagcagcat
      421 ctataaattc ttcagccttt atttctaaga cctgtgaacg cattaacctt gccgttccca
      481 tccagccaaa gacaatcaat gcaatcatta ctttatcaag accttttcct aatatagctg
      541 ttaaaactaa ggctgctact agaaatggta ttgacatgaa tatatccgtg accctcatta
      601 ttatttcatc aaaccagcca ccataatagg ctgatactgt tccaacaaaa atacctatta
      661 atgtacttat tcctacaact ataaatccta ttctaaatgc tgttctagag ccccaaacca
      721 taccataata aatatcatat gaaccctctg tagtaccaaa gatattatct gcatctggtg
      781 gtgtgggttg tatggtccag ccacttctag gtatattaaa tggttcatca ggatctgctg
      841 gtggtgcaag ccaaggagca aaaatagcaa ctacagcaaa aaagattacg attacaagac
      901 caaaaactga taatttattt gtgaataatc ttcttaatac atctgtccat tccatttttt
      961 acatcactcc aatctaattc taggatctat aaaggcgtat gatacatcaa caattaagtt
     1021 aattaatacc aaaatacctc caaagtacat agctactcct agaatacctg catagtctag
     1081 tcttgttgct gtttgatatg ctaatagtcc taaacctcta taaccaaata ccgtttctac
     1141 tgtcacaatt ccacctaaaa gtcctaatac cattggtcct gcaactgtta caacaggaat
     1201 taatgcattt cttctagcat gtttgtttat aacaacattt tcttttaccc ctttagcacg
     1261 agctgttcta atatagtctt ttcttaaagt ttcgagcata cttgatcttg ttattcttaa
     1321 gataaatgcc caccatacaa ctgctaatgt aaatacaggt gctactaggt gctgtaaagc
     1381 atctaatata aagtcaaaat taccattaaa tagaccatca attatattta aatcagtata
     1441 tgttataaat tcctgagtat ttttaagctc aatggcccag ttcgataacc gtccaggtgg
     1501 tgtccaccca aatactccat aaaatatcat taaaattatt aatccaaaaa caaacgtagg
     1561 gaacgaccaa cctactgtag aaaaaatccg tgttgcatga tcccaaaaat cattatgatg
     1621 aatcgccgag aagactccaa gccaaatacc acccattatt acgggtatca ccgccagaat
     1681 agcaagttcc aatgttgctg gaaatctatc cgttattgcc tggaccacag gtgcaccagc
     1741 tgcctctgac caacctaaat caccatgtaa tagtccgttt gcccatctac catattgcat
     1801 atagaagggt tcatctaagc catattttgt tattaacaga tccaaatctc cacctttaag
     1861 ctgagttgga tcagtaacat aagtagcaag tctttgatat ggtcccagca tcattaacat
     1921 accgaagact aacaatgtta caccaaatag tataagcggt aatattagta accgtcttac
     1981 aatataatta atcaactgtt aaaactcctt tctgtattac taaattttta cataagttat
     2041 attatcatag aattgccagg gggaatcccc ctggcaatat atattatcta aattgttaac
     2101 atttaactca ttttatttat ctaagatata gaagtattgt cctggacgca taggattgaa
     2161 gtaccatcct tttacccagt cgcgttgaat tcttgaacta aatggttgat ctaaccatac
     2221 atcaattgct tgatcatgtg aaatactttg aagttcttta tatagttcaa tacgtttttg
     2281 tgaatctagt tctccaatac cttgatcaat taatggatct acattttctt ctgcccattc
     2341 agcatagttc tctccacgtt tacctccata gtatccttgg gagtgtaaga aaggttgaac
     2401 aaagttatgt ggatctggga agtcagctaa ccatccacca atttgaagtg gaagtgtacc
     2461 tctagctgat tcatctaaga aagtagacca ctgaactgct cttgtatcaa tatcaaactt
     2521 aggattgatt tgctctaagt atgatttgaa gatatcagca gcagtttttc ttgtcatgtt
     2581 accagagtta taaaggatag taagtttaaa tcctttttcc caaacttctc cgtcccaagc
     2641 ttgtctgaag tgttctt
//
LOCUS       DATGCJ010000255        15806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_310141, whole genome shotgun sequence.
ACCESSION   DATGCJ010000255 DATGCJ010000000
VERSION     DATGCJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 15806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15806
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310141"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..803
                     /gene="deoC"
                     /locus_tag="VKN64_11195"
     CDS             120..803
                     /gene="deoC"
                     /locus_tag="VKN64_11195"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMA60842.1"
                     /translation="MAIKPRDMAKMIDHTNLNATATVEEIKQLCEEALEYQFASVCVN
                     PIYVPLATKLLEESSVKVCTVIGFPLGASTTETKAFETKNAIKNGAQEVDMVMNIGAF
                     KSGAYDMVRSDIKGVKDATKASGVTSDVIVKVILETCYLDEDEIVKACEIVKDVGADF
                     VKTSTGFGTAGAEEEKVSLMRKTVGRDIGVKASGGIKNFEQALSMLDAGANRIGASSG
                     VNIVTDIEE"
     gene            821..1594
                     /gene="recO"
                     /locus_tag="VKN64_11200"
     CDS             821..1594
                     /gene="recO"
                     /locus_tag="VKN64_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMA60843.1"
                     /translation="MALIETEAIVLKQFDLGEADKIITFYTKDAGKVRAVAKKARNSK
                     NNKVSAVVLPYCYNDIKVYKSKSLDRINNVESIHRFESLRNNLNKMAYAAYFAEMVEK
                     AGMEYHPNPQLFNLLLQTFYKMIRSSDLELEKINIVFKLLFLKYIGVQPRINYCYECG
                     KRYKTLKEQFFNVKEGGLICKKCAQNKNEELKSLRQNEVDLIKKIYRSELDSLEEITI
                     DKKILQRLDKLINEFIIYHLDFSLKSSDFLQIIKGFDKN"
     gene            1683..2567
                     /gene="glyQ"
                     /locus_tag="VKN64_11205"
     CDS             1683..2567
                     /gene="glyQ"
                     /locus_tag="VKN64_11205"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HMA60844.1"
                     /translation="MNFQDMIQNLHKFWSDHNCIIEQPYDTEVGAGTMSPSTFLRSLG
                     PEPWRTGYVQPSRRPADGRYGENPFRLQRYLQYQVIIKPSPDNIQEMYLESLKALGID
                     PNKHDIRFVEDNWEAPTLGAWGLGWEVWLDGMEITQFTYFQQVGGLDCKPVTVEITYG
                     LERIAMYLQDVDNIFDIEWVEGIDYGNVNLQIEKENSVYNFEESNVENLNQLFKIYED
                     EASRLIEKDLIFPAYDYTLKCSHVFNLLDARGAISVSERTGYISRVRDLARECAKGYV
                     KKREALDFPLLKGSDKDE"
     gene            2560..4638
                     /gene="glyS"
                     /locus_tag="VKN64_11210"
     CDS             2560..4638
                     /gene="glyS"
                     /locus_tag="VKN64_11210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HMA60845.1"
                     /translation="MSNRSLLEIGTEEIPAKLVKDIYSNMKEKMSELLEENRIEYENI
                     KVYGAPRRIAVEINGLSEFQKQEVNEIKGPPKNIAFDDEGDPTKAATGFAQSQNIEVD
                     ELEERDTERGIYLYAVVERERAETKELLKDLYKRLIKEVNLPITMRWSDHDLRFIRPI
                     HWLVSLYNDETIKFELEHLTASNETWGHRFLANKSFEVNSVDEYQEVLKKAKVIVDPQ
                     ERREIILEEIGTIILEKKLKFKLDNQLMDEVVHLVENPSPFLASFDEKYLELPEPVIV
                     TPLKEHQKYFPLWDQNDDLSNRFIVVKEGGKEYIDNVREGNEKVLKARLEDAEFYYQR
                     DIQEDLDQFLEDLNGLILREELGSMYDKTLRNVDLSEKIAANLNFAEDDIEKIKKAAR
                     YAKVDLVSQMVREFPELQGVMGSIYAYNNGMSNDVSLAIRDHYLPDSADSKLPGTELG
                     KIVSLSDKIDTLTGFFGINLIPTGSQDPYGLRRAAIGIFRLIEDLNYEVEIEKLFRYS
                     LEVFNQSQQVLKLEADELLNKIHEFMVDRIQYIFKDRGYDIDIIRTVIFGLPLSIPLI
                     KKAIRLLDEVKYNKSFQEFLTAFNRVDSIIKDIDQKEVKPDLFEKEEEENLYNKYKEI
                     STKVNKNLEQRELKQAYNHLIDIIDPIHDFFEEVRVMAEDERTQKNRLALLYNIQLLM
                     KSLGDFTYKQ"
     gene            4868..7531
                     /gene="ppdK"
                     /locus_tag="VKN64_11215"
     CDS             4868..7531
                     /gene="ppdK"
                     /locus_tag="VKN64_11215"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HMA60846.1"
                     /translation="MSEETKYVYFFGGGEADGRTGLKNLLGGKGANLAEMTNLGINVP
                     PGFTITTEVCTLYYENDENYPEGLEEEVEENLDRLENLMGKDFGDEEDPLLVSVRSGA
                     RESMPGMMDTVLNLGLNDTTVKGLAKQTGNERFAYDSYRRFVQMYGNVVLGMDGSDFE
                     EIIEEKKEEYDADVDTDLGVDALKELVKEFKAHIKEETGVDFPEDPKEQLWKAITAVF
                     GSWNNERAINYRNINDIPHHWGTAVNVQTMVYGNMGENCGTGVAFTRNPSTGEKKYYG
                     EYLKNAQGEDVVSGVRTPKDILELKDDMPHAYEQLVEIFEKLEQHYKDVQDVEFTIQQ
                     GELYMLQTRTGKRTAKAAIKIAVDMVDEGLIDKETAIQRIEPKKLDQLLHKRIDPDAD
                     VDLLATGLDASPGAASGEVIFDSDEAAERGDNGEKVILVRTETSPEDIHGMAAAQGVL
                     TSRGGMTSHAAVVARGMGKPAVAGCEAINVDYEKEEFIVDGRTIKKGENITIDGGNGE
                     VYYGIAPTIDPEMDENFERIMEWADDFRKIGVRTNADTPEDAAKAVEYGAEGIGLTRT
                     EHMFFGDERLPIVQDMILAQDKETRKEALDKLQPMQKEDFKGIFEAMGARPVTIRLLD
                     PPLHEFLPDHDELLVEATELRMTGDNPEKLEEKEKMLKAMERLEEMNPMLGHRGCRLG
                     ISYPEIYAMQVRAIFEAAGELKLEGKEVTPEVMIPLVSDISELKITKQVTKEAAEEVL
                     DELGVELEYSIGTMIELPRACLTADKIAEEAEFFSFGTNDLTQTTYGFSRDDAEGKFL
                     AKYIEDNVFENNPFQVLDQDGVGELMKIGVERGRHTRDDLKVGICGEHGGEPSSVKFC
                     HRAGLNYVSCSPFRVPIARLAAAQAQIEDQQ"
     gene            7635..8642
                     /locus_tag="VKN64_11220"
     CDS             7635..8642
                     /locus_tag="VKN64_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HMA60847.1"
                     /translation="MRDYTEEKEYRELSQYAAYSRESRGRRYKEEECDVRTCFQRDRD
                     RIIHSKAFRRLKHKTQVFIAPEGDHYRTRLTHTLEVSQIARTIARSLRLNEDLTEAIA
                     LGHDLGHTPFGHAGEEALCEVSSIDFSHNKQSLRVVDILEQRKKGIPGLNLTKEVRDG
                     IVNHTGDNSPLTLEGEIVRIADRIAYINHDIDDALRAGLIDIEDLPQEALDILGRNHS
                     KRIDTMVSDMINESRGQPEIKRSKEIYDATQSLRDFLFDNIYFGSLAKKEEDKAKRLV
                     KLLYQHYQANPSNIPAEFKNKIDDFSKEQIIIDYIAGMSDRYAIKVGKDIYIPEPWFV
                     K"
     gene            8761..10512
                     /gene="dnaG"
                     /locus_tag="VKN64_11225"
     CDS             8761..10512
                     /gene="dnaG"
                     /locus_tag="VKN64_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMA60848.1"
                     /translation="MSQNNGNFSEKVKDRLDIVEVVSEYVNLKKTGKNYQGLCPFHQE
                     KTPSFTVNPDNQFYYCFGCGKGGDIFNFIMEIENVTFYESLKLLAKKANLQMPDSRGY
                     SKEYNQKREKLFEIHQLAAKFYNYLLLNKQIGNEAFKYLSERGYTEKDLERYYIGYAP
                     DSWHSLYNFLSKRDFADQILKESGLIGHKNNNYYDKFRDRIMFPIFNSQGEVIAFGGR
                     RLDDEDKSSPKYYNSPETLIFHKSKNLYGINWAKKGFRNKNSAVIMEGYTDVITAHKY
                     GINNAVASLGTALTLDQAKILKRYVDTVYIAFDPDFAGEQATLKGLDILKEADLNVRV
                     IELPEESDPDDFIKDKGVDDFENLMESSYNLIEFKIRRIINEEEKYNINQRINRSKKV
                     LKVLSEINDPIERDVYLNKTADILNIDKEAMQEALNSFNQKNRQENEDKNRIKEKDSK
                     KELEDLNHQDLLLKTLIDNPERIKQAENELEPEYFQGIYRKAFNLILEKGFDILKTNI
                     EQEVDNQKLADYFMILTVKERQEVSEEKFNALLELMKDEYKKRAKNELFHKLQNVQLE
                     KSKLDNLLFDYHKIINI"
     gene            10530..11615
                     /gene="rpoD"
                     /locus_tag="VKN64_11230"
     CDS             10530..11615
                     /gene="rpoD"
                     /locus_tag="VKN64_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HMA60849.1"
                     /translation="MAKEDVKPENMKQVKELIARGKEEGELTYEEIMDSLEDIDLDSE
                     EIEEIYDIFAEMDIDIIEEGVDNDNDDDDDESELDLSIPQGVGIDDPVRMYLKEIGKV
                     DLLSAEEEVELAKRMEQGDEEAKRQLVEANLRLVVSIAKKYVGRGMLFLDLIQEGNMG
                     LMKAVEKFDYTKGYKFSTYATWWIRQAITRSIADQARTIRIPVHMVETINKLIRVSRQ
                     LLQEKGREPTPEEIGEEMDMSADKVREIKKIAQEPVSLETPIGEEEDSHLGDFIEDED
                     APAPASAASYMLLRDQLDNVLDTLTDREKRVLELRFGIEDGRPRTLEEVGKEFGVTRE
                     RIRQIEAKALRKLRHPSRSKKLKDYLS"
     gene            11771..11847
                     /locus_tag="VKN64_11235"
     tRNA            11771..11847
                     /locus_tag="VKN64_11235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11805..11807,aa:Ile,seq:cat)
     gene            11929..13044
                     /locus_tag="VKN64_11240"
     CDS             11929..13044
                     /locus_tag="VKN64_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMA60850.1"
                     /translation="MSNLQQIISLMAQKAPHKDAMDWDNVGLQVGDYASEIKKILIAL
                     DINEDVIQEAIKKETDLIITHHPLLFNGLDKVHSQTAKGRIVMQAIRNEIAIYSTHTN
                     MDIAEKGLNDFLADKLGIRDTEIFSKEKEKKLYKLAVYVPADNVEVLKEAIFKKGAGQ
                     IGNYSNTSFSSKGEGTFLPGENTNPHLGKRGEIKKVEEIKVETVVPEEKIDNVIKVML
                     KVHPYEEVAYDVYELPLQSDYKGIGRVGYLKESFPLKDYCEFIKDTLKIEKLKVRGNP
                     EEKIKKVAICSGAGADYISLASAKGADLYITGDVKYHEAQLAEELQLNLVDAGHFETE
                     VIFKEMIHQYLQEKIQKDKLEIKLELSEVNTNPWLYI"
     gene            13130..13696
                     /locus_tag="VKN64_11245"
     CDS             13130..13696
                     /locus_tag="VKN64_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA60851.1"
                     /translation="MTTNKKELIREAAIKVIARDGFYNTKTAKIAEEAEVAVGTIYNY
                     FNSKNEILEYIFEVEFEKRIDYLKKMQEKDYSIYKKLSLFSDKHFKEVRENLNTAQIL
                     VREKEFPKDSDFSSILNYLNELPSLLEKMLDKAVEQGELKCENTAVAASFIFGGLQGV
                     VEKALRTDNMSILDNAEKIVTQLLEKGL"
     gene            13769..>15806
                     /locus_tag="VKN64_11250"
     CDS             13769..>15806
                     /locus_tag="VKN64_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMA60852.1"
                     /translation="MLKINELVLKKPIITLIILFAITTFFGYQIYDKARIETNLEEYM
                     PENHPAFVKSDRYEDIFAINDSVVVAVENSNSIYNFATLKQIKEIGNRLAELEEINKG
                     EIKSLAKADNIEGSDLGLEIQPFFNKIPEEQTELKNLKNQVQDNNMVSGRLVAENHKV
                     ALIRAELAEGGADRIKLYNKIQRLVNNVEGPGKVYIAGQPIVEGTLATLMPQDMKVMI
                     PIVIFVISIVLLFTFSSIKSTLLTLGVVLFSTIWAFGLMAFFNIPIYAVSTMIPVMLI
                     ALGVADGIHLLIHLKDNLRNKVNISKEEAITDMVKNMWKPVVMTSITTAVGFISLLTS
                     EVYAVKYFGLFTAFGVMSAMVFSLIFIPAGLQIFNLPKISKKKSNSKGKTDKILSSFA
                     NWVLTHKKKIILISLIVFIAGIIGAQNLWINSSFLSKFEEDEEIKVANNFINKHFGGT
                     TNLNVIIKSENNDAIKNPTYLKDIWQLQKELEKLPEVGDSLALTDFLRRINKVMNEDQ
                     EKYNKVPDSRELVAQYLLLYSMSGDPDDLNKVVDYDYRRANLQINLKNDDARLISSVI
                     DKVESYQKDSSLSELEIDYAGSAYTNRVFARLILEGQIKSLVLSIIIVIILLAFLFKS
                     FKAGLLGSLPIIITALVNFGIMGYLNIALNTTTALISSIAVGMGIDYSIHLLSKY"
BASE COUNT         5773 a         2144 c         3267 g         4622 t
ORIGIN      
        1 gccttagcat caggaccttt tattacaact attgttgata ttaccgggct ttttatttat
       61 ttttatatgg cttcaatttt gattaataat tatttataat ggagaaggag tgatttctag
      121 tggcaattaa acctagagac atggcaaaaa tgattgatca tactaattta aatgcgaccg
      181 caactgtaga agagattaaa caattatgtg aggaggcatt agaataccag tttgcttctg
      241 tttgtgtaaa tccaatttat gtccctttag caactaaatt attggaggaa tcttcagtaa
      301 aggtctgtac agtaattgga tttcctttag gcgcttcaac tactgaaacc aaggcttttg
      361 aaaccaaaaa tgctattaaa aatggtgccc aggaagtcga tatggtaatg aatattgggg
      421 cctttaaatc aggcgcttat gatatggtta gatctgatat taagggtgtc aaagatgcaa
      481 caaaagcatc cggagtaact tctgatgtaa ttgttaaggt aattttagaa acttgttatt
      541 tagatgaaga tgagatagta aaagcatgcg aaattgttaa agatgtggga gcagattttg
      601 ttaaaacttc tactggcttt ggtaccgccg gagctgaaga agagaaggta agcttgatga
      661 gaaaaacagt tggtagagat atcggagtta aggcatcggg tggtattaaa aatttcgaac
      721 aggctctctc aatgttagac gcaggtgcta atagaattgg tgccagctcg ggggttaata
      781 ttgtaactga tattgaagaa taggaaagaa ggaagtatta atggctttaa ttgaaacaga
      841 ggctattgta ttaaaacagt ttgatttagg tgaagctgat aaaataatta ccttttatac
      901 aaaagatgca ggtaaggtga gggctgtagc taaaaaggcc cgcaattcga agaacaataa
      961 agtttcagct gttgtattac cttattgtta taatgatatt aaagtataca agagtaaatc
     1021 tcttgataga attaataatg tagaaagcat tcaccgtttt gagtctttga ggaataattt
     1081 gaataaaatg gcctatgccg cttattttgc agaaatggta gaaaaagccg gtatggagta
     1141 ccatcccaac ccgcaattat ttaatctttt attacaaact ttctataaga tgatcagaag
     1201 ttcagatctt gagcttgaaa agattaatat tgttttcaaa ttattatttt taaaatatat
     1261 aggtgttcaa cccaggatca attactgtta tgaatgtggt aagagataca aaacattaaa
     1321 agaacagttt tttaatgtca aagaaggcgg tttgatctgt aaaaaatgtg ctcaaaataa
     1381 aaatgaggaa cttaaaagtt taagacaaaa tgaagttgac ttaattaaaa aaatatatag
     1441 gtcagaatta gattctcttg aggaaattac tattgataaa aaaatcctac aaaggcttga
     1501 taaattaatc aatgaattta ttatctatca tttagatttc agcttgaaat caagtgattt
     1561 tttgcaaatc attaaaggat ttgataaaaa ttaaattaaa ggtagaaaca tctacctcgt
     1621 ctctgaccgg gatgaggtat ttgtattata taaaaaagat tcttttggaa ggaatgattt
     1681 taatgaattt tcaggatatg atacagaatt tacacaagtt ttggagtgat cataactgca
     1741 taatagaaca accctatgat acggaagtag gggcaggtac aatgagccct tcgacttttt
     1801 taagatcttt ggggcccgaa ccatggcgga ccggatatgt acagcccagc cggagaccgg
     1861 ctgatggtcg atatggagaa aatccatttc gtttacagag gtatttacag tatcaggtga
     1921 taattaaacc ttcacctgat aatattcagg agatgtattt agaaagtttg aaagcgttag
     1981 gcattgatcc caacaaacat gatattagat ttgtagaaga caactgggaa gcccccaccc
     2041 tgggtgcctg gggcttaggt tgggaagtat ggcttgatgg catggaaata actcagttca
     2101 cctattttca acaggtaggg ggacttgact gcaaaccggt aacggtcgaa attacttatg
     2161 gacttgagag aattgcaatg tacttacagg atgttgataa tatatttgat attgaatggg
     2221 tagaaggcat tgattatggt aatgttaact tacagattga aaaagaaaat tctgtatata
     2281 attttgaaga atctaatgtt gaaaatttaa atcaactatt taagatatat gaagatgaag
     2341 cttctcgact aatagaaaag gatttaatct tccctgctta tgactatacc ttaaaatgtt
     2401 cgcatgtatt taacctactg gatgcaaggg gtgcaatcag tgtttctgaa agaaccggct
     2461 atatttcacg ggtaagagat ttagcaagag aatgtgctaa agggtatgta aaaaagagag
     2521 aagccctaga tttcccttta cttaaaggaa gtgataaaga tgagtaaccg cagtctttta
     2581 gagattggaa cagaagaaat accggctaaa ttggttaaag atatctatag taatatgaaa
     2641 gagaaaatga gtgaactact agaagaaaac agaattgaat atgaaaatat taaagtatat
     2701 ggagcccccc gtagaatagc tgtagaaatt aatggtcttt ctgaatttca aaaacaggaa
     2761 gtaaatgaaa taaagggacc acctaaaaat attgcttttg atgatgaggg tgatcccact
     2821 aaagcagcta cgggttttgc ccaaagtcaa aatattgaag tagatgagct ggaggaaaga
     2881 gatacagagc ggggtattta cctttatgcc gttgtagaaa gagaaagggc cgagaccaaa
     2941 gaattattaa aagatctata taaaagatta attaaagaag ttaatttacc aattacaatg
     3001 agatggagtg accatgatct aagatttatt cgtcctattc actggctggt tagcctatat
     3061 aatgatgaaa cgattaaatt tgagttagaa catcttacag ccagtaatga aacctgggga
     3121 catagatttt tagctaataa gtcttttgaa gttaactctg ttgatgaata tcaagaagta
     3181 ctaaaaaaag ccaaagttat tgtagatccc caagaacgga gagaaataat cttagaagaa
     3241 attgggacaa taatcttaga gaagaaatta aagtttaaat tagataatca acttatggat
     3301 gaggtagttc atttagttga aaaccctagt ccctttttag cgagttttga tgagaagtat
     3361 ttggaattac cggaaccggt aattgttact cctttaaaag agcaccaaaa atactttccg
     3421 ctctgggatc aaaatgatga tttaagtaat agatttattg tagttaaaga aggcggtaaa
     3481 gagtatattg ataacgtacg agaaggtaat gaaaaagttc tcaaagccag gttagaagat
     3541 gccgaatttt attatcagag agatattcaa gaggaccttg accagttctt agaagatcta
     3601 aatggtttaa tattaagaga agaattaggc agtatgtatg ataaaactct tagaaatgtt
     3661 gatttaagtg aaaagattgc agctaattta aactttgctg aagatgatat tgaaaaaatt
     3721 aaaaaagcag cccgatatgc caaagttgat ttggtatccc agatggtaag ggaatttccc
     3781 gaattgcagg gagtgatggg gagtatatat gcctataata acggaatgag taatgacgtt
     3841 tccctggcta ttagagacca ttatcttccc gattctgcag atagtaaatt accgggtact
     3901 gaacttggta aaatcgtcag cctgtctgat aaaatcgata cattaaccgg attttttggt
     3961 atcaacttga ttccaaccgg gtcacaggac ccctatggat taagaagagc tgctatcggt
     4021 atttttagat taatagagga cttaaattat gaggttgaaa ttgagaagtt atttagatac
     4081 agcctggaag tgttcaatca atctcaacaa gtcttaaaac ttgaagctga tgaactatta
     4141 aataaaatac atgagttcat ggtagataga attcagtata ttttcaaaga caggggatat
     4201 gatattgata tcataagaac ggtcattttt ggtttaccac tttccattcc cttaataaaa
     4261 aaagcgattc gtttactgga tgaggttaag tataataaat cattccagga atttttaact
     4321 gcttttaata gggtggacag tataattaaa gatatcgacc aaaaagaagt gaagcctgat
     4381 ttatttgaaa aagaagaaga agaaaatcta tataataaat ataaggagat aagtacaaag
     4441 gtcaataaga atttagagca gagagaatta aaacaggctt ataatcattt aattgatatt
     4501 atagatccta tccatgactt ttttgaagaa gttagggtca tggccgagga tgaaagaaca
     4561 cagaaaaata ggttggctct tttatataat atacaattat taatgaagag tttaggtgat
     4621 ttcacctata aacaataaaa tttaaaaatt aggcagggat ttgtcaatta gtattgaata
     4681 ataataattg catgtaaaaa taattgtaat aaattacaaa aatataacct attaggaact
     4741 gagcagacag caggaggtgg ttagtcaact tatcataata aatttgattt agtatataaa
     4801 taatcttatt ataataatag taaggattgt aaagaatatt tgactaaata gaggaggtat
     4861 aaaattaatg tctgaagaaa ccaaatatgt atattttttc ggcggtggag aagcagatgg
     4921 tagaactggc cttaaaaact tattaggtgg taaaggtgca aatctagccg aaatgaccaa
     4981 tttaggaatt aatgtcccac ctggatttac aataactacc gaagtatgta cattatacta
     5041 tgagaatgat gaaaattatc cggaaggtct tgaagaagag gtcgaagaaa atcttgacag
     5101 actggaaaat ttaatgggga aagacttcgg agatgaagaa gaccctttat tagtttcagt
     5161 aagatctggt gcccgtgaat ctatgcctgg tatgatggac actgttttaa acttaggctt
     5221 aaatgatact acagtaaaag gtttggctaa acaaaccggt aatgaaagat ttgcttatga
     5281 ttcataccgt agatttgtcc aaatgtatgg taatgttgta ctaggtatgg acggcagcga
     5341 ctttgaagaa ataattgaag agaagaaaga agagtatgac gctgatgtag atactgacct
     5401 gggtgtggat gctttaaaag aattagtaaa agaatttaaa gcccatatta aagaagaaac
     5461 cggtgtagat tttcccgaag atcctaaaga acagctctgg aaagctatta ctgctgtatt
     5521 tggctcctgg aataatgaga gagctatcaa ctatagaaat ataaatgata ttcctcatca
     5581 ctggggtact gcagttaatg tacaaactat ggtctatggt aacatgggtg aaaactgtgg
     5641 aactggtgtt gcatttaccc gtaatccatc caccggtgaa aagaaatatt atggtgaata
     5701 tctaaagaac gctcagggcg aagatgttgt atctggtgtg cgtacaccaa aagatatttt
     5761 agaattaaaa gatgatatgc ctcatgcgta tgaacaatta gttgaaatct ttgagaaatt
     5821 agaacagcat tataaagatg tgcaggatgt tgagtttaca atccagcagg gcgaacttta
     5881 catgctgcag accagaactg gtaaaagaac agctaaagcc gccattaaaa ttgcggtaga
     5941 tatggttgat gaaggtctaa tagataaaga gaccgctatt caaaggattg aacctaaaaa
     6001 acttgaccag ttactccata agagaataga tcctgatgca gatgtagatc tcttagcaac
     6061 tggtttagac gcctctcctg gggctgcctc tggtgaagtt atctttgatt ccgatgaagc
     6121 agctgaaaga ggagataatg gagaaaaagt tatcttagtt agaactgaaa cctctccaga
     6181 agatattcac ggtatggcag ccgctcaagg tgttttaact tcccgtggtg gtatgacaag
     6241 tcatgctgct gttgtggccc gtggtatggg taaacctgcc gttgccggct gtgaagctat
     6301 taatgttgat tatgaaaaag aagaatttat cgttgatggt agaaccatca aaaaaggtga
     6361 aaatatcact atagatggtg gaaatggtga agtatattat ggtatagctc ctaccataga
     6421 tcctgaaatg gatgagaact tcgaaagaat tatggaatgg gctgatgact tcagaaaaat
     6481 tggtgttaga accaatgcag atactcctga agatgctgcc aaagcagttg aatatggggc
     6541 agaaggtatt ggtttaacaa gaacagagca catgttcttt ggtgatgagc gcctaccgat
     6601 tgttcaggat atgatcttgg cacaggataa ggaaacaaga aaagaagcac tcgacaaact
     6661 acagcctatg cagaaagaag acttcaaagg tatctttgaa gcaatgggag caagacctgt
     6721 tacaattagg ctacttgatc caccactgca tgaattctta ccagatcatg atgaactact
     6781 ggtagaagcg actgaattga gaatgacagg agataatcct gaaaaattag aagaaaaaga
     6841 aaagatgctt aaagcaatgg aacgcttaga agaaatgaac ccaatgctcg gacaccgcgg
     6901 ctgtagatta ggaattagtt atccagaaat ctacgctatg caggttaggg caatctttga
     6961 agcagcggga gaattaaaac ttgaaggtaa agaggtaaca cctgaagtta tgattccatt
     7021 ggtctctgat attagtgaat taaagattac taaacaagtt accaaggaag ctgctgaaga
     7081 agtcctcgat gaactaggtg tagagttaga atattcaatc ggtaccatga ttgaattacc
     7141 aagagcctgt ttaactgcag ataagattgc agaagaggct gagttcttct catttggtac
     7201 taatgaccta actcaaacca cctatggatt tagccgtgac gatgcagagg gtaaattcct
     7261 tgctaaatat attgaagaca acgtctttga aaacaatcca ttccaggttc ttgaccagga
     7321 tggagtcggc gaattaatga agattggtgt tgaaagaggt cgccatacaa gagatgactt
     7381 aaaagttggt atctgtggag aacacggtgg tgaaccaagt tcagttaagt tctgccatag
     7441 agctggactt aattatgttt cctgttcacc cttcagagtt ccaattgcta gattagctgc
     7501 tgctcaagct cagatagaag atcaacagta aattaagtat tttaataata taaaatactt
     7561 ttctgaagag gaagaatcca tgcttttaag tatgggttct tctttcttta atttttaaaa
     7621 aggggaaaat tattatgaga gattatacgg aggaaaaaga atatagagag ctgtctcaat
     7681 atgctgctta cagtcgagaa agtcgcggga gaaggtataa ggaagaagaa tgtgatgtaa
     7741 gaacctgttt ccaaagagat agagaccgta ttattcattc gaaagcgttt aggaggttga
     7801 aacacaaaac acaggttttt attgcaccag aaggtgatca ctaccggaca aggttaaccc
     7861 atactttaga agtctcacag atagcccgta ctatagccag gagtttaagg ctaaatgaag
     7921 atttaacaga ggctattgcc ttaggtcatg acttaggaca cactcccttt gggcatgcgg
     7981 gggaagaagc cttatgtgaa gtatcttcta tagatttttc tcataataag cagagtttaa
     8041 gagttgtaga tatcttagaa caaagaaaaa aaggaattcc cggtctgaat ttaacaaaag
     8101 aagtacggga cgggatagtt aaccatactg gtgataattc accccttact ttagaggggg
     8161 aaatagtaag aattgcagac agaatagctt atattaacca tgatatagat gatgcattgc
     8221 gggcgggttt gattgatatc gaggatctac cacaggaggc ccttgatatt ttaggaagga
     8281 accattctaa acggattgat accatggttt ctgacatgat caatgagagt aggggccagc
     8341 ctgaaatcaa gcggagtaaa gaaatctatg atgctactca aagtttacga gatttccttt
     8401 ttgataatat atattttggt tcacttgcta aaaaagaaga ggataaggca aaaagattag
     8461 taaaactttt ataccagcat taccaagcaa atccttctaa tataccagca gagtttaaaa
     8521 ataaaataga tgattttagc aaggaacaga ttataattga ttatattgcc gggatgtctg
     8581 accgctatgc gattaaagta ggtaaagata tctatatacc agaaccgtgg tttgttaaat
     8641 aaattattcg ttcatgcagg aatttgggtt tcactcctga ataaagcatt atgtgccgca
     8701 ataaatatga aggagtttag ttagtaataa agaatattat atatatcagg aatgattatt
     8761 atgagtcaaa ataatggaaa ttttagtgag aaagtaaaag acagattgga tatcgttgag
     8821 gtggtatctg aatatgtaaa tttaaagaaa actggaaaaa actatcaagg actctgtcct
     8881 tttcatcaag agaaaactcc atcttttaca gtaaatccag ataatcaatt ttattattgt
     8941 tttggttgtg gaaaaggtgg agatattttt aattttataa tggaaattga aaatgttacg
     9001 ttttatgaaa gccttaaatt attagccaag aaagctaatt tacagatgcc tgatagccga
     9061 ggttattcta aagaatataa ccaaaaaaga gaaaaactat ttgaaataca tcagcttgcc
     9121 gcaaagtttt ataattatct cttgttaaac aaacagattg gtaatgaagc atttaaatat
     9181 ctttcagagc gaggttatac tgaaaaagat ctagaaaggt attatattgg atatgcaccg
     9241 gatagctggc atagtttata caatttttta agcaaaagag attttgctga ccaaatatta
     9301 aaggaatctg gacttattgg tcataaaaat aataattatt acgataaatt tagagaccgg
     9361 ataatgtttc ctatttttaa ttcacaggga gaagttatcg cttttggcgg taggcgttta
     9421 gatgatgaag ataaaagttc tcccaaatat tataactcac cggaaacttt gatcttccat
     9481 aaaagtaaaa atctatatgg cataaattgg gctaaaaaag gttttcgaaa taaaaatagt
     9541 gctgtaataa tggaaggcta tactgatgta attacggccc ataaatatgg aataaacaat
     9601 gctgtagctt cactagggac agccttgacc ttagatcaag ctaaaatatt aaagagatac
     9661 gtagatacag tttatattgc ttttgatccc gattttgccg gtgagcaagc aactttaaaa
     9721 ggcttggata tcttaaaaga agccgattta aatgtaaggg taatcgaatt accagaagaa
     9781 tcagaccccg atgattttat aaaagataag ggtgtcgatg attttgagaa tttaatggaa
     9841 tcctcctata atttaataga gtttaaaata agaaggatta ttaatgagga agaaaaatat
     9901 aatattaatc agcggataaa ccgttccaaa aaagtactta aagtactttc tgagataaat
     9961 gatccgattg aacgtgatgt ttatttgaac aagaccgctg acattttaaa tatagataaa
    10021 gaagctatgc aagaagcttt aaattctttt aatcagaaaa ataggcagga aaacgaagat
    10081 aagaatagaa taaaagaaaa ggactccaaa aaagaattag aagatttaaa ccatcaggac
    10141 ttattattaa agaccctgat tgataacccg gaaagaatta aacaagcgga gaacgaactt
    10201 gaacctgaat attttcaagg gatatatcgg aaagctttta atttaatctt agaaaagggt
    10261 tttgatattt taaaaactaa tattgaacag gaagttgaca accaaaaact tgccgattac
    10321 tttatgattt tgacagttaa ggaaagacaa gaagtaagtg aagaaaagtt taatgctttg
    10381 cttgaactca tgaaagatga gtacaaaaaa agagccaaaa atgaactttt tcataaattg
    10441 caaaatgtac aattagaaaa aagtaaatta gataatcttt tatttgatta tcataagata
    10501 attaacatat aggaaaggag gtaatatata tggctaaaga agatgttaaa ccagaaaata
    10561 tgaaacaggt caaagagtta attgcccgcg gtaaagaaga aggcgaatta acttatgaag
    10621 aaattatgga ttcattggaa gatatagatt tagattctga agagattgaa gaaatctatg
    10681 atatttttgc tgaaatggat atagatataa tagaagaagg cgtagataac gataatgatg
    10741 atgacgatga tgaatctgag ctagatttaa gtatacccca gggggtaggc atagatgacc
    10801 ctgtccgtat gtatttaaag gaaattggta aggtggatct attatctgcc gaggaagaag
    10861 ttgaacttgc caagagaatg gaacagggtg atgaagaagc aaaacgacaa ttagtggaag
    10921 ctaacctcag attggtggtt agtattgcca aaaaatatgt gggaagaggc atgttatttt
    10981 tagacctgat ccaagaaggt aatatgggac ttatgaaggc agtagaaaaa tttgattata
    11041 ctaaaggtta taagttcagt acctatgcta cctggtggat tagacaggct attacacgtt
    11101 ctattgctga tcaagctaga accatacgaa tacctgtcca tatggtagag actataaata
    11161 aacttattcg ggtctcccga caactattac aggaaaaagg tagagaacct actcctgaag
    11221 aaatcgggga agaaatggat atgagtgcag acaaagtaag agaaatcaag aaaattgccc
    11281 aggaacctgt ttcattagaa acacccattg gggaagaaga ggacagtcat ctaggtgact
    11341 ttattgaaga tgaagatgcc ccggccccag cttcagcagc ttcctatatg ctgcttaggg
    11401 atcaattaga taatgtattg gatactctaa ccgatagaga aaaaagggta ttagaactta
    11461 gatttggtat tgaagatggt cgacccagaa ccttagaaga agtaggtaaa gaatttggag
    11521 taacaaggga aagaattaga cagatagagg ctaaagcctt aagaaaatta agacatccca
    11581 gtagaagtaa aaaacttaag gactatttga gttaattagt atttaagagt attaatgatt
    11641 atatagtagc aattataaga taatcaaatt ataatcataa taataggtaa tataagactt
    11701 tgaccttcaa agttttctgt catataatta cttttgtgtt acgaaataag catgaaagct
    11761 taatataaaa gggcctatag ctcagctggt cagagcaacc ggctcataac cggttagtcc
    11821 taggttcgaa tcctagtagg cccaccaaag taattaacct tgttctttag ataggacaag
    11881 gtttttttga ttttgaatat aatataatat atataagagg tggtataaat gagcaactta
    11941 caacagatca tatctttaat ggctcaaaaa gctccccata aagatgctat ggactgggat
    12001 aatgtaggtt tgcaggtggg agactatgcc agcgaaataa agaaaatatt gattgcatta
    12061 gacatcaatg aagatgtaat ccaggaagct ataaaaaaag agactgattt aattattacc
    12121 catcatcccc ttttattcaa tgggttagac aaagttcaca gtcaaactgc taaaggacga
    12181 atagtaatgc aggcgataag aaacgagata gccatttatt ctactcatac taatatggat
    12241 attgctgaaa aaggtttaaa cgatttttta gctgacaaac tgggcatacg agacaccgag
    12301 atcttttcaa aggaaaagga aaaaaagtta tataaattgg cagtttatgt cccagccgat
    12361 aatgttgaag ttctaaaaga ggcaattttt aaaaagggtg ccggtcagat tggtaactac
    12421 agcaacacct ccttttcttc taaaggagaa ggcaccttct taccgggaga aaatactaat
    12481 cctcatttgg gtaaaagagg tgaaattaaa aaggttgaag aaattaaagt agaaactgtt
    12541 gtaccggaag aaaagattga taatgtaata aaagtcatgt taaaagtaca tccctatgaa
    12601 gaagtcgcct atgatgttta tgaattgccc ctacagtccg attataaggg gatagggaga
    12661 gttggttatt taaaagaaag cttccccctt aaagattact gtgagtttat taaagacact
    12721 ttaaagatcg agaaattaaa agtgagaggt aacccggaag aaaaaattaa aaaggtagct
    12781 atctgtagtg gagcaggtgc tgattatatt agtttagcca gtgctaaagg ggctgattta
    12841 tatataactg gtgatgtgaa ataccatgaa gcccaattgg cagaagagtt acagttaaac
    12901 ttagttgatg ccggtcattt tgaaacagaa gtaatattta aagaaatgat tcatcaatat
    12961 ttacaggaaa aaattcaaaa agataaatta gagataaaat tagaactttc agaagtaaac
    13021 acaaacccct ggctttatat ttaacgagcg aggattagat cgatatttga caggaaaggg
    13081 gtattctgtt aaaatgaata ttgaatcatg attcaggaag ggttttaaaa tgactactaa
    13141 taagaaagag ctaattcgtg aagcggctat aaaagttatc gcaagggatg gtttttataa
    13201 taccaaaact gccaaaatag cagaagaggc ggaagtagcg gtaggtacga tctataatta
    13261 ttttaattct aaaaatgaga tattagaata tatttttgaa gtagagttcg aaaaacgcat
    13321 agattattta aaaaaaatgc aggaaaaaga ttatagtatt tataaaaaat tatctttatt
    13381 ttcagataaa catttcaaag aagtaagaga aaatctcaat acagcccaga ttttagtaag
    13441 agagaaggaa tttcccaaag atagcgattt ttcttcgatc ttaaactatt taaatgagtt
    13501 accctcctta ttagaaaaga tgttagataa agccgttgaa caaggggaac ttaaatgtga
    13561 aaatacggct gtagcagcta gtttcatctt tggtggactc cagggggtag tagaaaaagc
    13621 gctaagaact gataatatgt ctatcctaga caatgcagaa aaaattgtaa cacaattatt
    13681 agaaaagggg ctgtagtttt tttactataa tatgaatgat tgttcattca tattagttaa
    13741 aacactttat actaaaaggg gtaaaaatat gctaaagatt aatgaacttg tcttgaaaaa
    13801 acctattatt acattgataa ttctttttgc tattacaact ttttttggat atcaaatata
    13861 tgataaagcc cgtattgaaa caaacttaga agaatatatg ccggaaaacc atcccgcctt
    13921 tgtaaaaagt gaccgttatg aagatatctt tgccataaat gattcagtag tggtagcagt
    13981 tgaaaattct aatagtatct ataattttgc aactttaaaa cagataaaag aaattggcaa
    14041 tagattagct gagttagaag aaattaacaa gggggagata aaaagtctgg ctaaggcaga
    14101 taatattgaa ggttcagacc tgggtttaga aattcaacct ttctttaata agatacccga
    14161 ggagcaaact gaacttaaaa atctaaaaaa tcaggtacag gataataata tggtatcagg
    14221 tcgtttggtt gctgaaaacc ataaggtagc cttaattaga gcagagctcg ctgaaggtgg
    14281 agcagataga ataaaattgt ataacaagat acaaaggcta gtaaataatg tggaaggtcc
    14341 aggcaaggta tatattgctg gtcaaccgat tgtagagggt actttggcca ctctgatgcc
    14401 ccaggatatg aaagtgatga ttcccatagt tatcttcgtt atttcaatag ttttattatt
    14461 tacctttagt agtattaaga gtactttact tacattgggt gtagtattat tcagtaccat
    14521 atgggctttt ggcttaatgg catttttcaa tattccgata tatgctgttt ccacaatgat
    14581 accagttatg ttaatcgcct tgggagtagc tgatgggata caccttttaa ttcatttaaa
    14641 agataattta agaaataagg ttaatatatc taaggaagaa gctattaccg atatggttaa
    14701 gaacatgtgg aaaccggtgg ttatgacttc tataactact gcggtgggat ttatttcact
    14761 attaacatca gaagtatatg ctgtaaagta tttcggttta tttactgctt ttggcgtgat
    14821 gtcagcaatg gtattttccc taatattcat tccagccgga ttacaaatct ttaatctgcc
    14881 gaaaataagt aagaagaaaa gtaatagtaa agggaaaaca gataaaattt tatcatcttt
    14941 tgctaattgg gtcttaaccc ataagaaaaa gattattcta atctccttaa tagtttttat
    15001 agcgggaatt attggcgctc aaaatctctg gataaattct agttttttga gtaaatttga
    15061 agaagatgaa gagattaaag tcgcaaacaa tttcataaat aagcatttcg ggggtacaac
    15121 taatcttaat gtcataataa aatcagaaaa taatgatgcg ataaaaaacc cgacttattt
    15181 aaaggatatt tggcagcttc agaaagaatt agaaaaactt cctgaagtag gagacagttt
    15241 agctttgact gattttttaa gacggatcaa taaagttatg aatgaagacc aagagaagta
    15301 taataaggta ccggattcaa gagagttagt agctcaatat ctcttattgt attcaatgtc
    15361 aggtgatcct gatgacctaa ataaagttgt cgactatgat tatagaaggg ctaatttaca
    15421 gataaattta aagaatgatg atgctagatt aatatcttca gtaattgata aagtagaaag
    15481 ttatcaaaaa gattctagtt tatctgaact tgaaatagat tatgccggct ctgcttatac
    15541 taaccgagta tttgcccgat taatcttaga aggtcagata aaaagcctgg ttttatcaat
    15601 aattatagtg attatcctgc ttgcctttct ttttaaaagt tttaaagccg gtttgttggg
    15661 aagtttaccc atcataataa cagccctcgt taatttcggg attatgggtt atctaaatat
    15721 agccttaaac accactacag ccttgatttc tagtattgcg gtaggcatgg gaatagacta
    15781 ttctattcac ctactctcca aatata
//
LOCUS       DATGCJ010000256         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_476568, whole genome shotgun sequence.
ACCESSION   DATGCJ010000256 DATGCJ010000000
VERSION     DATGCJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476568"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VKN64_11255"
     CDS             complement(<1..825)
                     /locus_tag="VKN64_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMA60853.1"
                     /translation="MKKFVVFLLVLTMLFSFSALAAASEEGKLLIWADENRIKVLNSV
                     KDQFEDEYGIPVEIQEKAFGDIRDNMAVEAPSGEGPDIIVGAHDWLGALVSNGLIAPI
                     ELGDKRDDFYDVAINAFSWGEDVYAVPYAIEAVGLIYNKDIVPDPPTSFSAFEAIVRN
                     ITDKEEEMYGFIMPQPDPYHTFPFMSGPGGYVFGTNEEGVLDPNDVGLNNEGAVLGLQ
                     KLDNLYEDGVVPFVDYQTMTSLFTNGQVGMMLTGPWALPALQEAGIDYGFTQLPKIE"
     gene            complement(981..2366)
                     /locus_tag="VKN64_11260"
     CDS             complement(981..2366)
                     /locus_tag="VKN64_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMA60854.1"
                     /translation="MPKGLNVPPYKIKMVEPINLIPKEEREAALKRAGYNLFSLKSEE
                     VFIDLLTDSGTNAMSDNQWAGIMQGDESYAGSKNFYHLKDTVKDIFDYNYIVPAHQGR
                     GAEQVLFPLVVKKGNYVLSNMHFDTTKAHVELAGGRAKNFVIDEALDTEKYHPFKGNF
                     DVEKMENFIKEKGKDKISMIIITITCNSAGGQPVSMENIKKVSNLAKKYELPIIMDAA
                     RFAENAFFIKSRVQKYEDHSIKEIIKEMFSYTDAFTMSAKKDAIVNMGGIIGIKDNEE
                     LYNKVRSQAVPFEGFPTYGGLAGRDMEAIARGLKEGIEYPYLKHRIDQISLLGKKLKS
                     NGIPVQYPVGGHAVFVDARKLLPHIPYDQFPAQALANELYLEGGIRGVEIGSLLLGRD
                     PETKEQLKSPFEFLRLTIPRRVYTDSHLNFVAESLINIKDRIKDIKGLTFTYEPEILR
                     HFTARLKPVEE"
     gene            2497..3747
                     /locus_tag="VKN64_11265"
     CDS             2497..3747
                     /locus_tag="VKN64_11265"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMA60855.1"
                     /translation="MSKNCLVAQSGGPTPIINASLQGVIETAKSSNEIDKVYGARFGI
                     NGLMDELICDLTAEAEQEIKLLKHTPGSILGSCRYKLNKLDIDKTDYEKIFKIFKKYD
                     INYFFYIGGNDSMDTVAKLNQYAQFKDIDLTVMGIPKTIDNDLLITDHSPGFGSAAKY
                     IATSVMETTLDSRVYQRKNVHLIEVMGRHAGWLAAASALAGETGLQTPDLIYFPEITF
                     STDKFVQDLQEKAKTKDNIIVVVSEGIKDESGNYISAGESSKHDQFGHKQMGGAARSL
                     VPYIKEYVYERVKVIEFNVLQRAANHVASKVDAEEAYACGSQAVEFAVKENATGKAVV
                     IKRVSNDPYKSKIELADVDKIANQEKKFPREWINKEGNHVTEEALEYLRPLAQGEMAR
                     KTVNGLPRYSNIYAAVTTKPKAEL"
     misc_binding    3811..4036
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4092..>6074
                     /locus_tag="VKN64_11270"
     CDS             4092..>6074
                     /locus_tag="VKN64_11270"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMA60856.1"
                     /translation="MENLPKNYDPNKVEDKWYDYWMENGLFEAEVDRSKEPFSIVMPP
                     PNITGQLHMGHALDNTLQDILTRWKRMKSFASLWLPGTDHASIATEVKVVDKLRKEGI
                     DKDELGREGFLDKAWEWKDEYGNRITNQLKKMGSSCDWSRERFTMDEGCSEAVKEVFI
                     QLYEKGLIYQGDYIVNWCPSCHTTLSDVEVEHEEHEGKLYYIKYPIKDSDEFITVATT
                     RPETMLGDTAIAVNPSDERYKDLVGETVILPLMEREIPVIADPFVDSGFGTGMVKVTP
                     AHDPNDFDMGERNDLEVVKVIDEDANMTEVTGKYAGMDRYECREEVIKDLKEQDLLVK
                     IDEHEHSVGQCYRCDTVIEPLVSKQWFVKMEPLAEPAIAAVKEGDIKFIPERFSKVYL
                     NWMENIKDWCISRQLWWGHRIPVWYCQDCGEVIVRREEPNECPNCGSSDLKQDEDVLD
                     TWFSSALWPFSTMGWPEETEDLDFFYPTDVLVTGRDIIFFWVARMIFMGLEFMDEVPF
                     DDVYVHGLIRDAQGRKMSKSLGNGIDPIEVIEEYGADALRFTLITGNTPGSDMRFREE
                     RLEASRNFANKIWNASRFILMNLEDFDYSSIKEEDLKLNLADKWMLSRTNEVTQKIDD
                     AMERYSFGEMSKSLYDFIWNEYCDWYIEILKERLYQD"
BASE COUNT         1961 a         1051 c         1254 g         1808 t
ORIGIN      
        1 ttcgatttta ggtaactggg taaatccata gtcaataccc gcttcttgta aagctggtaa
       61 agcccaggga ccggtaagca tcataccaac ttgaccattt gtaaataaac tggtcatagt
      121 ttggtaatca acgaaaggaa caactccatc ttcataaagg ttatctagtt tttgtagacc
      181 taaaacagca ccttcattat ttagtccaac atcattagga tccagaacgc cctcttcatt
      241 agttccaaat acataaccac cggggccact cataaagggg aaggtatggt agggatcagg
      301 ttgaggcatg atgaatccat acatttcttc ttccttatca gtaatgtttc tgacaattgc
      361 ttcaaaggcg gagaaacttg taggtggatc aggtacaata tccttattat aaataagtcc
      421 tactgcctca atagcatagg gtactgcata aacatcttca ccccagctga aagcattaat
      481 tgcaacatca tagaaatcat ctcttttatc tcctaattcg atcggagcga taagaccatt
      541 agatacgagt gcgcctaacc agtcgtgagc accaacaatg atgtctgggc cttcaccgga
      601 aggggcttct acggccatat tatcacggat atctccaaag gctttttcct gaatttcaac
      661 aggaataccg tactcgtctt cgaattgatc tttaactgag tttaatactt tgatccggtt
      721 ttcatcagcc cagattaaaa gttttccttc ttctgaagct gcagctaagg cgctaaaact
      781 aaatagcata gtaagaacta gtaaaaaaac gacaaacttt ttcataaatc tttcacctcc
      841 ataaaattta ccagatctta aaaataatat atctggttgt atataatata ttctatcaaa
      901 gtaattatta tcctttaata ttaatcatat attcagaaaa aaatttatgg aaatgaaagt
      961 ggatatttta agttaaaaat ttactcttca actggtttta aacgggcggt gaaatgtctc
     1021 aaaatttctg gttcataggt aaaggtaagt ccttttatat ctttaattct atctttaata
     1081 ttaattaatg attcagccac aaagtttaag tgggaatcgg tataaaccct tctcggaata
     1141 gtaagtctta aaaattcaaa aggtgacttt agttgttcct ttgtttcagg atctctacct
     1201 agaagtaaag aaccgatttc aactcctcta ataccgccct ctaaataaag ttcattggct
     1261 aatgcttgag cggggaactg gtcataagga atatgaggta gtaacttgcg ggcatctaca
     1321 aaaacagcgt gtcccccgac cggatattga acaggaattc cattactttt taacttttta
     1381 ccaagtaagg aaatttgatc gattctgtgt tttaggtacg gatattcaat accttctttc
     1441 agccccctgg caatagcttc catatctcgt ccggctaaac cgccgtaagt gggaaagcct
     1501 tcaaaaggta cagcctgaga ccttacttta ttatatagtt cttcattatc ttttataccg
     1561 attataccgc ccatattgac gatagcatct tttttggcac tcatcgtaaa agcgtcagta
     1621 taagagaaca tttctttgat gatctcttta atagaatgat cttcatattt ttgtaccctt
     1681 gatttaatga aaaaggcatt ttctgcaaaa cgggccgcat ccataattat cggcagctca
     1741 tattttttag ctaaattact gactttttta atattttcca ttgaaactgg ttgaccacca
     1801 gcactattac aggtgatagt gattattatc atcgatattt tatctttacc tttttcttta
     1861 ataaagtttt ccattttttc tacatcaaaa ttacctttaa agggatgata tttttcggta
     1921 tctaaggcct catcaatcac aaagtttttg gcccgccccc cggccagttc cacatgtgct
     1981 tttgtagtat caaaatgcat gttagataag acataatttc cctttttcac cacaagaggg
     2041 aagagaacct gttcagcccc ccgaccctgg tgggcaggta ctatgtaatt gtaatcgaag
     2101 atgtctttga cagtatcttt taggtgatag aaatttttac taccggcata ggattcatca
     2161 ccctgcatga tgcctgccca ctgattgtca ctcatcgcgt ttgtgccgct atctgttaaa
     2221 agatcgatga atacttcctc tgactttaga gagaacaggt tgtaaccggc tctttttaaa
     2281 gccgcctctc tttcttcttt aggtattaaa ttaataggtt caaccatctt aatcttatat
     2341 ggtggtacgt ttagtccttt tggcaaaata atcactccca aatataatat attcacatat
     2401 taatattttc attttaaaag gataaaatcc tttatttatt ctctgattaa tggtaaatta
     2461 tataatagaa agcatactat taaaggggtg tattttatga gtaaaaactg tctagtagcg
     2521 caatcagggg gtcctacccc aataattaat gccagcctac agggagttat tgagactgcg
     2581 aaaagtagta atgagattga taaagtttat ggagcaagat ttggtatcaa tggtttaatg
     2641 gatgaattaa tttgtgattt aaccgcagaa gcagaacaag aaattaaatt attaaaacat
     2701 acaccgggtt ccattttagg gagctgtcga tataagctta ataaattaga tattgataaa
     2761 actgattatg aaaagatatt taagatattt aagaaatatg atatcaatta tttcttctat
     2821 atcggtggca atgattccat ggatactgtt gctaaattga accaatatgc tcaatttaaa
     2881 gatattgatt tgacagtgat gggtattcct aaaactattg ataatgacct cttaattact
     2941 gaccattcac cgggatttgg cagtgctgca aaatacatag ccaccagtgt catggaaaca
     3001 actctagaca gccgggtata ccaaagaaag aatgtccact tgattgaagt gatgggtaga
     3061 catgctgggt ggctggcggc cgctagtgct ttggcaggag agaccggctt acaaactccg
     3121 gacttaatct attttccaga aataaccttt tctaccgata agtttgttca ggatttacaa
     3181 gaaaaagcta aaacaaaaga taacatcatc gtggtagttt cggaaggtat taaggatgaa
     3241 agcggtaatt atatcagtgc aggagagtct tctaagcatg atcaatttgg tcataagcag
     3301 atgggaggag cagctcgttc cctagtacca tatataaaag agtatgtata tgaaagagtc
     3361 aaagtgatag aatttaacgt actgcagaga gcggccaatc atgttgcctc taaggtagat
     3421 gcagaagagg cttatgcctg tggaagccag gccgttgaat ttgcagtcaa agaaaatgcg
     3481 accggcaaag cagttgtaat aaaaagagtt tctaacgatc cttataaatc aaaaatagaa
     3541 ctagccgatg ttgataaaat agccaatcag gaaaaaaaat tcccgcggga atggatcaat
     3601 aaagaaggta accatgtaac tgaggaagct ttagaatact taagaccatt agcgcagggt
     3661 gagatggccc gaaaaacggt taatggtctg ccgagatatt caaatattta tgcagctgtc
     3721 accactaaac caaaagctga actttaagtg caactttctt gacaaaccgt ccaaataata
     3781 gtattattat atacaaatat ttataaatat ttaatgactt tgaaagggag gagtaatttt
     3841 agcggagtcc cagagagaga aacccaccgg ctgtaaggtt tcttgacaca agctaaaatg
     3901 aaggtcgccc cggagttgtt tgtctgagat gagtagggca aaccggactt aaccgttata
     3961 ttaaaagagg gtctggttag tttactagcc aggaactaag gtggtaacgc gagaaaaatc
     4021 ctttttcgtc cttaattgga tggggaagga ttttttatat tttaaaaata tttacaggag
     4081 gtatgatgaa gatggaaaac ttaccaaaaa actatgatcc taataaagta gaagacaaat
     4141 ggtacgatta ctggatggaa aacggcttat ttgaagcaga ggttgacagg agtaaagaac
     4201 ctttttcaat agtaatgccg cccccgaata ttaccggtca gttacatatg ggacatgctc
     4261 tggataatac cctacaggat attttaacaa ggtggaagag gatgaaaagc tttgcttccc
     4321 tctggctgcc tggtactgac cacgccagta ttgctacaga ggttaaagta gttgataaat
     4381 taagaaaaga aggtattgat aaagatgaac tcgggcgaga aggtttttta gataaggcct
     4441 gggaatggaa agatgagtac ggcaatagaa taactaatca gttaaaaaag atgggttcat
     4501 cctgtgattg gtctcgtgaa aggtttacga tggatgaagg ttgttcggaa gctgtaaaag
     4561 aagtttttat ccaactatat gaaaaaggat taatctatca gggagactat atcgtaaact
     4621 ggtgtcccag ctgccataca actttatctg atgtagaggt agaacatgaa gagcatgagg
     4681 gtaagctata ttatattaaa tatccgatta aagatagtga tgaatttata actgtagcta
     4741 ccaccagacc ggaaaccatg ttaggtgata ctgcgatcgc ggttaacccg tctgatgaaa
     4801 ggtacaagga tttagtggga gaaaccgtaa ttttaccttt aatggaaaga gaaataccgg
     4861 taattgccga tccctttgta gacagtggat tcggaacagg tatggttaag gttacacctg
     4921 cccatgatcc caatgacttc gatatgggag agcgaaatga tttagaagtg gttaaggtta
     4981 tcgatgaaga tgccaacatg actgaagtta ccggtaaata tgctggcatg gaccgctatg
     5041 aatgtagaga agaagttatt aaagacttaa aagagcagga cctcctagta aaaatagatg
     5101 aacacgaaca ttccgttggt cagtgttacc gctgtgacac agttattgaa cccctggttt
     5161 caaaacagtg gttcgttaag atggaacctc tggctgaacc ggctattgca gctgttaaag
     5221 aaggggatat taaatttatc ccggaaaggt ttagtaaggt ttatctaaac tggatggaaa
     5281 atattaaaga ctggtgtatc tcccgacagc tctggtgggg acatagaatc cccgtctggt
     5341 actgtcagga ctgtggtgaa gttatagtta gaagggaaga accaaacgaa tgtcctaact
     5401 gcggcagcag tgatttaaaa caggatgaag atgttttaga cacctggttt tcttctgccc
     5461 tctggccgtt ttcaaccatg ggttggccgg aagaaactga ggatttagac ttcttttatc
     5521 cgaccgatgt tctcgttaca ggtagagaca ttatcttttt ctgggtagct agaatgatct
     5581 tcatgggact tgaatttatg gacgaagtac cctttgatga tgtttatgtt cacggtctaa
     5641 ttagagatgc tcagggccga aaaatgagta aatcattggg gaacggtata gatcccatag
     5701 aagttattga agaatatggg gcagatgcac tcaggtttac cctaattaca ggtaataccc
     5761 caggtagtga catgcggttt agggaagaaa ggctggaagc cagccgtaac tttgctaaca
     5821 agatctggaa cgcttcccgc tttatattaa tgaacttaga agattttgat tatagctcaa
     5881 ttaaggaaga agatttaaag ttaaatcttg ctgataaatg gatgttaagc aggaccaatg
     5941 aagtaacaca aaagatagat gatgcgatgg aaagatatag tttcggtgaa atgagtaaga
     6001 gcctttatga ctttatctgg aatgaatact gtgattggta tattgagatt ttaaaagaga
     6061 gactctatca ggat
//
LOCUS       DATGCJ010000257        12908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_87135, whole genome shotgun sequence.
ACCESSION   DATGCJ010000257 DATGCJ010000000
VERSION     DATGCJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87135"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VKN64_11275"
     CDS             <1..1561
                     /locus_tag="VKN64_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60857.1"
                     /translation="IYYNAQNSMNIRNKKLVYILQLILLAAIIAPAIYFSLVTYQAFA
                     QLNQPELVITSMYVNAVILMFFLGIPIIVSVFFFSKDIRFLTALPIEEEKIIFAKLST
                     VYIYLLAIGAAFILPSIIIYAIKTGISLSLIILGPIAFLLAPLLPLLISSILVLTFNK
                     IIRKSKYKNLLTMVGNMLLIVVIIMMQLNITQKAADPAVIKNAFLNNESLITLIGLRF
                     PPSIWMTKMLLGSFTNTLYFILINIIFVMALHFLARYFFKKSLLTFTEGSRSNGKIRY
                     KKASPGWQLFKRHMLIIIKEPTFLLNALLSMVVPLIIYVIMSFSGQFSIEILNSAQIK
                     PFMVLIYSGIMVFPAIVSNISSTVITREGRAFWQTKVLPISDKMNIKYRIMTTLVINL
                     SGSLILGLIGYILLPVNLNMVLVASFFTISLTLFLANIDFKINILRPLLNWSHPTAAV
                     KNNMNVTMTLGIRLILGLILFGFYKLSGNLFNNFNSIVYILSIVFLILYFISRYYLYN
                     IMVNRFKKISL"
     gene            1909..3342
                     /locus_tag="VKN64_11280"
     CDS             1909..3342
                     /locus_tag="VKN64_11280"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HMA60858.1"
                     /translation="MRNLTLMTDFYELTMAQGYFKSDKMEKAVFDYFYRENPDDNGYV
                     IFSGLEQVIDYAKNLEFNEDDLAYLQKQGLDPEFLEYLSNFSFTGDIYAVPEGSVVFP
                     KEPILKVEATILQAQLLESAILNIINHQSLIATKAARIVKAAGERKVLEFGLRRAHGP
                     DAGIYGARAAVIAGCTGTSNVLAGKEFDINISGTHAHSWVMSFDEEIEAFRTYAENFP
                     DSCILLVDTYDTLESGVPNAIKVFEEMKEKGINPDLYGIRLDSGDLAYLSKQSRQMLD
                     EAGFEDAVIVASSNLDEDIITSLDFQGAEIDVFGVGTKLITSKSDPALGGVYKLTQYR
                     GEPKIKISDNVDKITNPGSKKLLRVYDKKERKIIADLIALEDENIDEREDLILFDDRD
                     TWKTKTLKGGNYQIRSMLTPIFDNGNCVYESPSVKEIKEYCKQEKESLWAEYRRLTNP
                     HIMKVDLSSKLYDLKQNLLHENGIENA"
     gene            3856..4272
                     /locus_tag="VKN64_11285"
     CDS             3856..4272
                     /locus_tag="VKN64_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HMA60859.1"
                     /translation="MVPDIIPVWVGVIIYQFGPFPLDLPSVFWVVLVIVTIITVLSDF
                     LANAIAVKKSGGSNVSVIAALVGLAIGLVILGPLGIIIGPFAAIFLAEYYRSQKKDYN
                     EPLKIAFSTVFAFIGSGIVKIVLLLIVIVWFFILVL"
     gene            4294..4926
                     /locus_tag="VKN64_11290"
     CDS             4294..4926
                     /locus_tag="VKN64_11290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60860.1"
                     /translation="MECKLCGSANIKKINIEDKYYFCENCELIFIEKAAEPSAKQEYE
                     IYQSHENTPENEGYVNMFKDFINELLAEHLDEIEEVLEYGCGPGPVLADILENKGLNV
                     TKYDPFFYPEVEFKDKTYDLITSTEVFEHFQNPKEEVEKLLKLMNPGAYLAVMTSFHE
                     GIEHFKDWWYRRDSTHITFYNLKTFRYIENNYPLKIIKTDKENSILLKKE"
     gene            4996..6063
                     /locus_tag="VKN64_11295"
     CDS             4996..6063
                     /locus_tag="VKN64_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60861.1"
                     /translation="MEICLKIYKFYNLGTTLRKNEIMNNLKETGIENMETYDFEENLE
                     NPSFVSYPVPIEFTYNLREKDDKVIGKVFDYGAMSMMTEIKYEVNSLEEFHEEIKKYD
                     PRREIEKDFDSIYNKISSNFEGLSDEILSSFNHLYSVYCIDNKDNINKKELIENNEKI
                     IVSLLSDEYEDINFSQQQKEAVMRYNNSFLERDYIVVHWNNAFILDKKENYPEKLFMI
                     ELANVQFNKLQTYDRFIDEYLSEYLLNKEKSALRFIPFIKSGLSKKIKEIADLRVELE
                     RVIDVMDNFEKFQGQWYLARLYYLANGAFQITRWRNLVENRINKVNELYNLINSEINR
                     KRMLFLNVLMLLLFILWFLGI"
     gene            6245..7867
                     /locus_tag="VKN64_11300"
     CDS             6245..7867
                     /locus_tag="VKN64_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HMA60862.1"
                     /translation="MKKVYLYLTILIMCFSFLTLSTVAISAQTNLNYEIEEQLSLFKE
                     YELSNLKIRPPYFLNELVNLYEKNNFNPFWFDETWFKDSAYQLIEVIESIEYEGLNPR
                     DYRYDYLSKYFHKTTLNRQERAIVDILMTNSFLLLTSHFSQGVLDSETLKRNWLDIKN
                     KIDLYNFLNSSIHNNNIIESLYSLLPRTENYIQLRNKLKEYKKIKENGGFIQFTSTRL
                     IDKGMSGEDILNLKRRLKQSKDFQGAMDEDFSQLLEEAVKNFQMRHGLKADGIVGPET
                     KESLNVSIDEKIKKITINMERLRWLPQTLGNPYIFVNIADYKLKVVENNEELFNIKVI
                     VGKRQRSTPVFSDEISYIVLNPYWTVPKKIAVEDKLQLIKKDINYLAENNYKVLKVSN
                     GNLVEVDYSKIDWQKLNRDNFNYFLRQDPGPNNALGQIKFMFPNKYSVYLHDTPSKEL
                     FLEEQRNFSSGCIRLQEPFKLAEYILKKNNKWDNVKINEILATDKEEVIYLAKRIPIH
                     IVYMTAWVNDNYQIQFRNDIYQRDQKLIEAYY"
     gene            7884..8825
                     /locus_tag="VKN64_11305"
     CDS             7884..8825
                     /locus_tag="VKN64_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HMA60863.1"
                     /translation="MIKKISIVLILLVFLSIPIQAFDEGRTNFQLVYNDSLISHEIYS
                     IFILPGENITLNIYKPSSNNNYILRHNNLKIVIENNYEWVLKAPQQVGNYLVQVINEK
                     TREKIDLNVFVLEPFNNLENGYLNGYKIGDYPHIPPSKRDNYSLPKGFIKVTRQNKDT
                     YLTPHFKLSQFICKQPGNYPKYLVMQELLLDKLEYLLLKVNQKGIKADTFHVMSGYRT
                     PFYNSSLGNVRFSRHIFGDAADIYIDVYPKDGLMDDLNGDGRIDLRDADILYDTVEES
                     YKRGEYKEYIGGLSSYRRTVSHSPFIHIDTRGYKARW"
     gene            8929..10824
                     /locus_tag="VKN64_11310"
     CDS             8929..10824
                     /locus_tag="VKN64_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HMA60864.1"
                     /translation="MEKNEILTKFNFSEEVTEVIKECPSFNLVEDSEKLRELSLANSE
                     NGQKEVTYKISNDREVVDARVCKVRNGIAANFMEPYMRRRDPDCMVIGDDYPTDKERF
                     KERYGEEFADTRQETFAWLKNQPLAAFFFEAGPENLNYSVMAIAPANAGFFAFGLSLL
                     QGIKKLDELDAGVRPKGYIFVAPPFRYTHFNGDQVVVHNRLKGNYELFSYNLYPGPSA
                     KKGVYSMLINLGEKNEWPTLHCSAVQVVTPYDNKLNIAYEGASGAGKSEMLQDMHRER
                     SGRIKLGENIVTGDEYKITLPQGCSLRPIADDMAVTPSYLQKGNGKLTLKDAENAWFV
                     RVDHIDQYGVDPHLESITCHPESPLLFLNIDAAPNSTALIWEHIEDEPGKPCPNPRVV
                     IPRNDVTGVIKGEVNIDVRGFGVRTPPCTKENPTYGIFGLFHVLPPALAWLWRLVSPR
                     GYGNPSINDSEGMSSEGVGSYWPFATGRKVDQANILLKQIVDSPEVKYILCPNQYIGA
                     WEVGFMPEWIAREYLARRGGAWFTDKQIEPARSPLLGYALKDVMVEGQMIERELLKVQ
                     NQPEVGVEAFDQGAQILREFFEDYLQNYDRKELLPLGQKIIDCFLSKGTLEDYMSLID
                     YPVIKED"
     gene            10949..11584
                     /locus_tag="VKN64_11315"
     CDS             10949..11584
                     /locus_tag="VKN64_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA60865.1"
                     /translation="MFELKNVKYKDILEIEELTINESEITCIVGRSGGGKSTFLRLLN
                     NMVSADKGEILFKGESIEKYNPIKLRREVLMLPQNPVIFSGTIRDNFTITLNYNNEYA
                     MADENYDKILEKVGLKEQALDDEAEKLSGGEKQRLALARILILDPDILLLDEPSSALD
                     EETERLIIEMVVKCIKEKNGTLIMVTHSQEIAEKYGDRIITINEGELDRLN"
     gene            11600..12385
                     /gene="fetB"
                     /locus_tag="VKN64_11320"
     CDS             11600..12385
                     /gene="fetB"
                     /locus_tag="VKN64_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HMA60866.1"
                     /translation="MTDTIFDISIINLVIAYIFVLILIFIVKNENIGHEKQIIIASFR
                     MTVQLVLVGYVLTYVFENPNPYITILILMVMEVFAIFNIYSRVESKLSSNLKVIIGIS
                     MFGGTLASILFFLFIVINIEPWFMPQYFIPISGMLIGNSMTGISLGAQGLITGINDNK
                     ERIENALMLGATPEKATKDIQNRSFYNAVLPTINSMIGMGIVFLPGMMTGQILAGAAP
                     LTAIKYQIAIMMGILGSVTLTVYLMVKWGMKSFFNERAQIHID"
     gene            12413..12673
                     /locus_tag="VKN64_11325"
     CDS             12413..12673
                     /locus_tag="VKN64_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60867.1"
                     /translation="MNETKVVLILIDKRTDAALKVQKVLTAKGCMIKTRLGVHETSSC
                     AEEGLIFLNVEGSDAEVNELLEELNNLKRVKAELVKMSLPAQ"
BASE COUNT         4558 a         1770 c         2439 g         4141 t
ORIGIN      
        1 tatatattat aatgctcaaa atagtatgaa tataagaaat aaaaaactgg tatatatttt
       61 acaactgata ttgttagccg caattatagc tccagctatt tactttagcc tggtaactta
      121 tcaggcattt gcccagttaa atcagcctga actggttatt acatctatgt atgttaatgc
      181 tgtaatatta atgttttttc tcgggattcc aatcattgtt tctgtcttct ttttttctaa
      241 ggatattaga tttttaactg cattaccaat tgaagaagaa aaaataatat ttgctaaatt
      301 aagtacagtt tatatctatc tattggcaat aggtgccgcc tttattttac cgagtattat
      361 tatctatgct attaagactg gtattagttt aagtttaatt attttaggac cgatagcttt
      421 tttattggct cccttattac cccttttaat atcatcaatt cttgttttaa cattcaataa
      481 aattatacga aagagtaagt ataaaaacct attgacaatg gtcggcaata tgttattaat
      541 tgtggtaatt atcatgatgc agttgaatat tacacaaaaa gcggctgatc ccgccgttat
      601 aaaaaatgca tttctaaata atgaaagtct gataactttg attggactaa gatttcctcc
      661 cagtatctgg atgaccaaaa tgttattggg ttcttttaca aatacactat attttattct
      721 aataaacata atatttgtaa tggcactgca ttttttagca agatatttct ttaaaaaatc
      781 tctattaacc tttacagagg ggagtagaag caatggtaaa ataagataca aaaaagcaag
      841 tccgggttgg cagttattta aaaggcacat gcttattatt ataaaggaac caaccttttt
      901 acttaatgcc cttttaagta tggttgtccc tttaataatc tatgtaataa tgagttttag
      961 cggccaattt tcaattgaaa tattgaattc tgctcaaatt aagcctttta tggttttaat
     1021 atacagcggg ataatggttt ttccagccat agtttccaat atatcttcga ctgtcataac
     1081 gagagaaggg agagcattct ggcagaccaa agttttaccg atcagtgata aaatgaatat
     1141 taaatataga ataatgacta ccttagtaat aaatttatcc ggttccttaa tattaggttt
     1201 aattggatat attctattac ctgttaattt aaatatggta ttagtagcca gtttttttac
     1261 tatctcattg accctgtttt tagctaacat cgatttcaag atcaatattt taaggccttt
     1321 gttaaactgg agtcatccta ctgccgctgt taaaaataat atgaatgtga caatgacctt
     1381 gggaataaga ttaatattag gtttaatctt atttggattt tataaactga gcggtaatct
     1441 attcaacaac tttaattcaa ttgtctatat cttaagtatt gtgtttttaa tattgtattt
     1501 cataagtaga tattatctat acaatataat ggtgaataga tttaaaaaga tttccctctg
     1561 atttcaaata tataaaagtt aaatttccat ttatatattg taaagaatta ggcaggcaat
     1621 tagtatttaa ttgaattcga ataaaaaatg acacactttg taaacctaga gcaaataatt
     1681 ttcttaggtt cactccgtgt gtcaaattta ttttaaacta aagaatttta aaaatctaat
     1741 ctgtgtttat tcaaataaat ttatattgca catcaaaaat tataaaattt gttctatttg
     1801 taatcaattt aaccagttga tataatttaa atggaataat tggtttaaat tgtggggatt
     1861 aatttttaaa taagcctaaa ttatattatt taaaaagggg taaataatat gagaaattta
     1921 acattaatga ccgactttta tgaactaaca atggcacaag gatattttaa aagcgataaa
     1981 atggaaaaag cagtattcga ttatttttat cgggaaaatc cggatgataa tggttatgtt
     2041 attttcagtg gtttagaaca ggtaatagac tatgctaaaa atctagaatt taatgaggat
     2101 gatcttgcct atctacaaaa acagggtctt gaccctgaat ttttagagta tttaagtaat
     2161 ttttctttta cgggtgatat atatgctgtt ccggaaggca gtgttgtctt cccaaaagaa
     2221 ccaattttaa aagtagaagc aaccatttta caggctcagt tactagagtc agccatatta
     2281 aacataatta atcaccaatc tttgatagct actaaagctg ctcgcatcgt taaagcagcg
     2341 ggtgagagaa aagttcttga atttggcttg cgaagggccc acggcccgga tgctggcata
     2401 tatggagcac gagctgctgt tatcgccggc tgtacaggga cttctaatgt cctggctggg
     2461 aaagaatttg atataaatat tagtggtact catgcccaca gctgggtgat gagttttgat
     2521 gaggaaattg aagcctttcg tacatatgca gaaaactttc ctgacagctg tattttactg
     2581 gtagatactt atgatacact tgaatcgggt gttcccaatg ctataaaagt atttgaggaa
     2641 atgaaagaaa aaggtattaa ccctgattta tatggtataa gattagatag tggtgacttg
     2701 gcttatttat ctaaacagtc ccgtcagatg cttgatgaag ccggttttga agatgcagtt
     2761 atagttgcgt ccagtaacct tgatgaagat attataacct ccctggattt tcagggggca
     2821 gaaattgatg tttttggggt ggggactaag ctaataactt ccaaaagtga tcctgcccta
     2881 gggggagtct ataaattaac tcaataccgg ggggaaccca aaataaaaat ttcggataat
     2941 gtcgataaga taacaaatcc aggttcgaaa aaattgttga gggtttatga taaaaaagaa
     3001 agaaagatca tagctgatct aattgcgtta gaagatgaaa atattgatga gcgagaagat
     3061 ttgattttat ttgatgaccg cgatacctgg aaaaccaaaa ctctaaaagg gggcaattat
     3121 caaatccgct caatgttaac ccccattttt gataatggaa attgtgttta tgaatctcct
     3181 tctgttaaag agattaaaga atactgtaag caggaaaaag aatctttatg ggctgaatat
     3241 aggcgtttaa caaaccctca tattatgaaa gtagatcttt caagcaaact ttatgattta
     3301 aaacagaatc ttcttcacga aaatggaatt gagaatgctt gacctgattt atcagaaagt
     3361 aaaacccggt ggttttaaga tctcaacttc tctcacctaa tcagagacaa gcataagaaa
     3421 gagcttgtta aactaaaaac cacactcagt aaaagtcttt aacaaacaat aaaccttctc
     3481 tatctcaaac tgggggattc aaaaatagag aaagttgaag ggcatttgac taaaagaccc
     3541 tatccctacg gtaaagacaa gagaggctac aggttaccct ataggttatc ataggatagc
     3601 tcttttagct atatagttaa ttatagtatt gatgccaata aattaccgta gtttttgtga
     3661 gataagtcta tatcaagttt ttatataaaa gattcctcat attttatgag ggatttttgt
     3721 atttacagaa attaatgata aaataataat aataataaaa taaagataat tgaaaggaga
     3781 attaatttgc tcagtatata ttggatactc atagttatat ttttcttaat gggtcttatt
     3841 ggggtttttg tgccgatggt acccgatatt attcccgtct gggtaggtgt tatcatatat
     3901 cagtttggtc cttttccact tgatctaccc tctgtttttt gggttgtctt agttatagta
     3961 actataataa ccgtattatc tgatttttta gcaaatgcga tagctgtaaa aaaatcaggt
     4021 ggtagtaatg tttcagtaat cgctgccctg gtgggattag cgataggtct agttatatta
     4081 gggccccttg gtattattat aggacctttt gctgcaatat ttttagctga gtattacaga
     4141 agccaaaaaa aggactataa tgagccctta aagattgctt tttctaccgt ttttgccttt
     4201 ataggtagtg ggatcgttaa gatagttctt ttattgatag ttatagtatg gtttttcatt
     4261 ttagtactct aaaataatga tagggatgat aatatggaat gcaagttatg tggtagtgca
     4321 aatattaaaa agatcaatat tgaagataag tattatttct gtgaaaactg tgagctgatt
     4381 tttattgaga aagcagctga accttctgcc aaacaggagt atgaaatata tcaaagtcat
     4441 gaaaacacac ctgaaaatga gggctatgtt aatatgttta aagattttat caatgaatta
     4501 ttggctgagc atctagatga aatagaggaa gttttagaat acggctgtgg gccgggtccg
     4561 gttttagctg atatattgga aaacaaaggt ctaaatgtta caaagtatga tcctttcttt
     4621 tatccggaag ttgaattcaa agataaaact tatgatttaa taacttctac tgaagtgttt
     4681 gaacattttc agaatccgaa agaagaggtt gaaaagctgc ttaaattaat gaatcccggt
     4741 gcttatttag ctgtgatgac atcttttcat gagggtattg aacatttcaa agactggtgg
     4801 tatagaagag atagtaccca tataactttt tataatttaa agacatttag atatatagaa
     4861 aataattatc ctttaaaaat aataaagacc gataaagaaa acagtatctt acttaaaaaa
     4921 gaataatcta tagtttgcaa tatacaaaca tttgtcttaa tctaataaat gaaatgttcg
     4981 gaggaggtca gttatatgga gatttgtctt aagatatata aattctataa cttaggtact
     5041 actcttagaa aaaatgagat aatgaataat ttaaaagaga ccggtattga gaacatggag
     5101 acctatgatt tcgaagaaaa ccttgagaac cccagttttg tctcataccc ggtaccgata
     5161 gaatttactt ataatctgag agaaaaagat gataaggtta ttggcaaagt ttttgattat
     5221 ggagcaatgt caatgatgac agagataaaa tatgaggtga attcactcga agagtttcat
     5281 gaagaaatta aaaaatatga tccccgtagg gagatagaaa aggattttga tagtatatat
     5341 aataaaattt ccagtaattt tgaaggctta agtgatgaga ttttaagcag ttttaaccat
     5401 ttatactccg tttactgtat agataataag gataatataa ataaaaaaga attaatagaa
     5461 aacaatgaaa agatcatagt gagtttatta tccgacgagt atgaagatat caatttcagc
     5521 cagcagcaaa aagaggcggt tatgcgctac aataacagct ttttagaaag agattatatt
     5581 gttgttcact ggaataatgc ttttatttta gataaaaaag aaaattatcc ggaaaaactt
     5641 ttcatgatag aattggccaa tgtccagttt aacaaactac agacctatga tagatttatt
     5701 gatgaatacc tatcagaata tttattaaac aaagaaaaga gcgcattaag gtttattccc
     5761 tttatcaagt cgggcctgag taaaaagata aaagaaatag cagatttaag ggttgaatta
     5821 gaacgagtta tagacgttat ggataatttc gagaagtttc agggccagtg gtatctagcc
     5881 cgactctatt atttagccaa tggtgctttt caaattaccc gctggcgtaa tctagtagaa
     5941 aaccgtatta acaaggtgaa cgagctttat aatcttataa attctgaaat aaatagaaaa
     6001 cgtatgttat tccttaatgt attaatgtta ttattattca tattatggtt tttaggtatt
     6061 tagttcttca aatatattta aataaaacca aggcttgtga taaatgggaa aaaatacatt
     6121 tgacttaagg aaaaatatta tttactattg tatatatata ataaatacaa ataaatatct
     6181 ttgagttggt tttatttaaa tatggaaaaa tattgaatac ggtcttcaat aaagaaggga
     6241 tattatgaaa aaagtttatt tgtatttaac aattttaata atgtgtttct cttttttgac
     6301 tttatcaact gtagctatat cagctcaaac taatcttaat tacgaaatcg aagaacagct
     6361 ttccctattt aaagaatatg aattaagtaa tttaaagatt agaccaccgt attttttaaa
     6421 tgagctagta aatttatatg aaaaaaacaa ctttaaccct ttttggtttg atgaaacctg
     6481 gtttaaagac tctgcttacc agctaattga agtaatagaa agtatagaat atgaaggttt
     6541 aaatcctcgc gattacagat atgattatct ttctaagtat ttccataaaa caacccttaa
     6601 caggcaggag agggccatag ttgatatatt aatgacaaat tcctttttat tacttacctc
     6661 tcatttcagt caaggtgtgc tcgattctga aaccttaaaa agaaattggc tggatatcaa
     6721 aaataagatt gatctttata actttttaaa tagttctata cataataaca atattattga
     6781 atctctctat tctttactgc ctcgaacaga aaattatatc cagctgagaa ataagttaaa
     6841 agaatataaa aaaattaaag agaacggtgg atttattcaa tttaccagca cgagattaat
     6901 tgataaagga atgtcggggg aagatatcct taatttaaaa cgcagactta aacaaagcaa
     6961 agattttcag ggggctatgg atgaagactt tagtcagcta ttagaagaag ctgtaaaaaa
     7021 ctttcaaatg cgtcatggtt taaaagcaga tgggatagta ggccctgaaa caaaagaaag
     7081 cttaaatgta agtatcgatg aaaagatcaa aaagataacg attaacatgg aaagattaag
     7141 atggctgccg caaactttag gtaatcccta tatatttgta aatattgcag attataaatt
     7201 aaaagttgtt gaaaataatg aagagctatt taacattaaa gttattgtgg gtaaaagaca
     7261 gagaagtaca cctgtgttca gtgatgaaat tagttatatt gttttaaatc cttattggac
     7321 tgttcctaaa aaaatagcag tagaggataa attacaatta ataaaaaagg atataaatta
     7381 cctggccgag aataattaca aagtattaaa agtgtcaaat ggtaatttag ttgaagttga
     7441 ttatagcaaa atcgattggc aaaagttaaa tcgagataat tttaactact ttttaagaca
     7501 ggatccgggg ccaaataatg ccttaggcca aattaaattt atgtttccca ataagtacag
     7561 tgtttatttg catgatactc ctagtaagga gttattttta gaggaacaga gaaactttag
     7621 ttcaggctgt ataagattac aagagccctt taagcttgcc gagtatatat tgaaaaaaaa
     7681 taataaatgg gataatgtca aaattaatga aattttagcc actgataaag aagaggtaat
     7741 ctacctggcg aaaagaatac caattcatat cgtctatatg acagcctggg taaatgacaa
     7801 ctatcaaata caattcagaa atgatatcta ccagcgagat caaaaattaa ttgaagcata
     7861 ctactaaaag aaagaggtaa acaatgatta aaaaaatatc aatagtttta attttactag
     7921 tgtttttatc aatacccata caagcatttg atgaaggtag aaccaacttt caattggtct
     7981 ataacgattc attaatttct catgaaattt atagtatatt tattctaccg ggggagaaca
     8041 taacattaaa tatttataag ccatcttcta ataataatta tattttaaga cataacaatc
     8101 ttaaaatcgt catagaaaat aattatgaat gggtattaaa ggcgccccag caagtaggta
     8161 actatttggt tcaagtaatt aatgaaaaaa caagggagaa aatagacctg aatgtttttg
     8221 ttttagaacc attcaataat ctagaaaatg gttatttaaa tggttataaa attggcgatt
     8281 atcctcacat tccgcccagt aaaagagata attacagttt accgaaaggt tttatcaagg
     8341 tgactcgaca aaataaagat acatatttaa caccccattt taagttaagt cagtttatct
     8401 gtaaacaacc cggtaattat cccaagtatc tggttatgca ggaattatta ttagataaat
     8461 tagagtattt acttttaaag gtaaaccaaa agggaattaa ggcagatact tttcatgtga
     8521 tgagcggtta tcggacccct ttttataata gttccctggg taatgtaagg ttcagccgtc
     8581 atatctttgg agacgcagct gatatctata tagatgttta tcctaaagat ggtctcatgg
     8641 atgatctaaa tggggatgga cgtatagact taagagacgc tgatatactt tatgacacag
     8701 tagaggagtc ttataagagg ggagagtata aagagtatat cggtgggtta agctcctatc
     8761 gtagaacagt ttctcacagc ccttttattc atattgatac caggggatat aaggcccgct
     8821 ggtagaagtt caaataaatt tattcacctt taaaaagttc aacaccacta gttttggtgt
     8881 tgaattttta attaatttta aaaaaaagta gaaagtaggg agtatgatat ggaaaaaaac
     8941 gagattttaa ctaagtttaa tttttcagaa gaggttacag aagttatcaa agaatgtccc
     9001 agctttaatt tagttgagga tagcgagaaa ttaagagaat tatcattagc aaatagtgaa
     9061 aatggccaaa aagaggttac gtacaaaatt agcaatgatc gggaagtagt tgatgcgcga
     9121 gtatgtaaag taagaaatgg tatagctgcc aactttatgg aaccttacat gcgaagaaga
     9181 gatccggact gtatggtaat tggtgatgat tatccgactg ataaagaaag atttaaagaa
     9241 aggtacggag aggaatttgc agataccaga caggaaacct ttgcttggct aaaaaaccaa
     9301 cctttggctg cttttttctt cgaggcaggg ccggagaatt taaattattc tgtaatggca
     9361 attgcccccg ctaatgctgg tttttttgcc tttggtttat ctttactcca gggaattaaa
     9421 aaattagatg aattagatgc gggagttaga ccaaaaggat atatctttgt agctccacct
     9481 tttagatata ctcattttaa tggagaccag gttgtagtac ataataggtt aaagggtaat
     9541 tatgaacttt ttagttataa cttatacccc ggccctagtg ctaaaaaagg tgtctacagt
     9601 atgttgatta atctggggga gaaaaacgaa tggccgacct tacactgttc ggccgtacag
     9661 gtagtaaccc cttatgataa taagttaaat atagcttatg aaggggcaag tggagccggc
     9721 aaatcagaga tgctgcagga catgcaccgg gagagaagtg gtaggattaa attgggtgaa
     9781 aatatagtaa ccggggatga gtataaaata actctacccc agggctgtag tttaaggccg
     9841 attgccgatg atatggcagt tactccttct tatctgcaaa aaggtaatgg gaagcttact
     9901 ttaaaagatg cggaaaatgc ctggtttgtc agggtagacc atattgatca atatggggta
     9961 gacccccatt tagaatcaat tacctgtcat ccggaaagcc ccttattatt tttaaatata
    10021 gatgctgctc ccaacagtac agctttaatc tgggaacata ttgaagatga gccgggtaaa
    10081 ccatgtccta atccccgggt tgtaatacca cgaaatgatg taactggtgt cattaaaggc
    10141 gaagttaata tagatgtaag gggatttggt gtgagaacac ctccctgtac caaagaaaat
    10201 ccaacttacg gtatttttgg cttattccat gtgttaccac cggccttagc ctggttgtgg
    10261 agattagttt caccacgcgg ctatggtaat cccagcatca atgattcgga aggtatgagt
    10321 tcagaagggg taggttcata ctggcctttt gccaccggtc gtaaagttga ccaggccaat
    10381 atattactta aacagatcgt tgattcaccg gaggtaaagt atattttatg tcccaatcag
    10441 tatattgggg cctgggaagt aggttttatg ccggaatgga tcgccagaga atatctggcc
    10501 agacgggggg gagcctggtt cactgacaaa cagatagagc cggcccgatc accactttta
    10561 ggctatgctt taaaagatgt gatggtagaa ggtcaaatga tagagcggga gctcttaaag
    10621 gtacaaaacc aaccggaagt aggagtagaa gcttttgacc agggagccca aattttaaga
    10681 gaattctttg aggattactt gcagaattat gaccgtaaag aacttttacc attagggcaa
    10741 aagattattg attgtttcct atcaaaagga actttagaag actatatgtc attaattgat
    10801 tatccggtta taaaagagga ctaaaagtta gattattaga cttcagctgt aaaaagctgg
    10861 agttttttct ttttataaag gaatacgtta tatattattg aatatataat attgataaga
    10921 ttgatttcct ttaaaaggga gggcttacat gtttgaatta aaaaatgtca aatataaaga
    10981 tattctagaa atagaagagt taaccattaa tgaaagtgaa atcacctgta tcgtcggccg
    11041 cagtgggggc ggaaaatcta cctttttgag gctgctgaac aatatggtaa gtgctgataa
    11101 gggggagatt ttatttaagg gagaaagtat tgaaaaatat aaccccataa aattaagaag
    11161 agaagtttta atgctccctc aaaatcctgt cattttttcg ggtacgataa gagataactt
    11221 tactattact ttaaattata ataatgaata tgcgatggcc gatgaaaatt atgataagat
    11281 cttagaaaaa gttggactta aagaacaggc tttagatgat gaggctgaaa aactttcggg
    11341 gggagaaaaa cagagattag ccctggcccg aattttaatt cttgatcccg acatcttact
    11401 cttagatgaa ccttcttccg ctttggatga agaaactgaa agattaatta ttgagatggt
    11461 agtaaaatgt ataaaagaaa agaacgggac cttaattatg gttactcact cccaggaaat
    11521 agctgagaaa tatggggacc ggataattac cataaacgaa ggtgaacttg atagattaaa
    11581 ttaataagga gagaattata tgactgacac tatttttgat atatctataa taaatttggt
    11641 aattgcctat atttttgtcc ttattttaat ttttattgta aaaaatgaaa atataggaca
    11701 tgaaaaacag attataattg cttcctttag aatgaccgtg caattagtat tagtagggta
    11761 tgtattgact tatgtatttg aaaaccccaa tccatatatt actatcttaa tcctgatggt
    11821 aatggaagta tttgctattt tcaatatata ttccagggta gaaagtaaat tatcgagcaa
    11881 tttaaaggtt atcatcggaa tttccatgtt cgggggtact ttagccagta tattattctt
    11941 tttatttatc gttatcaata ttgaaccctg gtttatgccc cagtacttca tcccgatctc
    12001 tggaatgctg attggtaatt ccatgaccgg aatttcgctg ggagcacagg gcttaattac
    12061 aggcataaat gataataaag aaaggattga gaatgccttg atgttagggg cgacacccga
    12121 aaaagcaacc aaagatattc aaaataggtc cttttataat gcggttttac cgacaataaa
    12181 ttctatgatc ggaatgggta tcgtattttt accaggtatg atgaccggcc agattttagc
    12241 aggagctgcc cccttaacag caattaaata tcagattgct atcatgatgg gtatcttagg
    12301 tagtgttact ttaaccgtgt acctaatggt gaaatgggga atgaaaagtt tctttaatga
    12361 aagagctcag atccatattg actaaaaata agggttaaag gaggttgttt tgatgaacga
    12421 gacaaaggtt gttttaattc ttattgacaa gagaacggat gctgctttaa aggttcagaa
    12481 agtattgaca gcaaagggtt gtatgataaa gacaagatta ggagtccatg agaccagttc
    12541 ctgtgcagaa gaggggttaa tatttttaaa tgtagaagga agtgatgcag aggttaatga
    12601 actattggaa gagcttaata atttaaaaag ggtaaaggct gaattggtta agatgtcact
    12661 acctgcccaa taattcaaaa tttcctccct tagtgtaaaa taacttttgg agattaagaa
    12721 taaaaaaata gaaagaagac tccctttctg ataaaatgtt tttagtgaca aaaacaaaat
    12781 tgaaaggaga gtcttcttat gaataatatt atacaacatt ttcatgaaaa aatcacgttt
    12841 gagctaggaa aagaagtaga aaacattttt ttaaatggtg aaggtgatat cacaaaaata
    12901 attaattt
//
LOCUS       DATGCJ010000258        17419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_311092, whole genome shotgun sequence.
ACCESSION   DATGCJ010000258 DATGCJ010000000
VERSION     DATGCJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311092"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1194)
                     /locus_tag="VKN64_11330"
     CDS             complement(97..1194)
                     /locus_tag="VKN64_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMA60868.1"
                     /translation="MKLVYKYIAKELIMPFFFGVAAFVGIFIGTDLLYRLTDFYSNWG
                     ISIITLIELFFLSLPSIIVLTFPMATLLAIIMAYSRLSNDSEITAFRAGGVSIYRLVI
                     PAVVLGLIMSFFAIGLNEYVVPQSNYLYDKIVWEFKHGEKLPNTQYNLYLTPLDDQTN
                     RPDYILYTHQFDGDTGIMHDVYLQDYEEGAPTVLIQAERAEWLETGWKFFNGKIFYLR
                     TGERIPALEFDEYNAVQIFNRPQEISKLNKKVEDMNFNELQEYINLKESQGREVLEEK
                     VKLHQRLSIPFASFIFALLAAPLGIKPGRSSGSAVGMGISIIVIFIYYIMMTIGDALG
                     SQGTVAPWLGAWLSNIIFLIVGSVLLYRVAR"
     gene            complement(1282..2001)
                     /gene="lptB"
                     /locus_tag="VKN64_11335"
     CDS             complement(1282..2001)
                     /gene="lptB"
                     /locus_tag="VKN64_11335"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMA60869.1"
                     /translation="MTITAKELVKKYDNRRVVNKVNLRVNKGEIVGLLGPNGAGKTTT
                     FYMIVGLIKPDHGNVHLDEEDLTHQPMYQRAKKGIGYLSQEPSVFRKMSVYNNIKAVL
                     ELRDLTDEEMKSRIEELLDEFHLNKIRDSYGYQLSGGERRRVEIARSLAIEPSFILLD
                     EPFTGIDPIAVSEIQKIIMYLKEKGLGVLITDHSVRETLSITDRAYIMYEGDILMSGN
                     SEEIAESELAREFYLGDEFQI"
     gene            complement(2009..2632)
                     /gene="lptC"
                     /locus_tag="VKN64_11340"
     CDS             complement(2009..2632)
                     /gene="lptC"
                     /locus_tag="VKN64_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMA60870.1"
                     /translation="MNKLFQKKTAIISFIIILFLFNINVLAQKNLTADKLTFKKSEEG
                     TNIIEANNNVKLIYNEITITGDYALYKQAEELINFDGNVNITMENKNLKGNHLKYNLN
                     EERLNMNENVQISAEDIKGNSNKLSYNRNNNIIELTGKAHIEYENLKADADKIVIYEN
                     EDRAVLTGNVSGTQNGNEFQAEQIEIFDRGNRIEMQGNAQLNIISEE"
     gene            complement(2639..3205)
                     /gene="lptC"
                     /locus_tag="VKN64_11345"
     CDS             complement(2639..3205)
                     /gene="lptC"
                     /locus_tag="VKN64_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMA60871.1"
                     /translation="MYLKNKRKLILIFLMIIILIAGISLTIYYEINKDEETVVEREQQ
                     DQQEYLTQIDLELFNNDKTIKWNVNSEKLTRENNGSIYKMDILEFTAFENEELIYTGR
                     GDKANYNKNKELFSFTGDININRDDSILQTNQIVWNRKNDIIKGEGGVRFETSRFVIT
                     SEKFSAPLSLNKIEFIGNEDAKAKVEWR"
     gene            complement(3189..4340)
                     /gene="lpxB"
                     /locus_tag="VKN64_11350"
     CDS             complement(3189..4340)
                     /gene="lpxB"
                     /locus_tag="VKN64_11350"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMA60872.1"
                     /translation="MTGKIMVIAGEISGDERAAEVVQKIQELNPHVNFFGMGTTKLKE
                     QGVEVVIDPTELESVGIWEVIKNYQIHKEHYKVMKDIAKKRDPDVLFLVDYSGFNMFM
                     TRLGHKMDIPVVNYFPPSAWLWGEWRAKFLARYNPVVAANFPMDRDVYRRNKAETKFV
                     GHPLLDEINVEAEKDELKNTFELKENEIPIALLPGSRKAELEKLLPIMLKTAVKLRED
                     NKDYVFIIPSADPKFNDFIEKNVIDYPLKVKIVNNHTHKLLKMSHFAIVSTGTATLEA
                     MILNTPMVIIYKFSDVTYKMFKYLSNQEFFSMPNIIAEKKIVPELMQEEVTPEKIYEE
                     AHYILSKPYLMTDIKIKLVSAAEKLGSQGAIDKTARLVMREGNIDVLKK"
     gene            complement(4342..5157)
                     /gene="lpxI"
                     /locus_tag="VKN64_11355"
     CDS             complement(4342..5157)
                     /gene="lpxI"
                     /locus_tag="VKN64_11355"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HMA60873.1"
                     /translation="MNNIGLIAGYGKLPILWAKKAKEHGVKIHAFPIKEEYSEELRDY
                     SDTIDYISVTQYNALLNKLKKYNIENIIMIGKVNKKHLYEKKEFDQRFLKLINDTDNR
                     EDNSILEKLLAEFKKEGIKVLKQNLFMDDLLVSAGQFNNIKPDDSLLEEMKYAFKKAK
                     QIANYNIGQTVLTNNKAVVAVEAIEGTDQAIKRAGNLVKEGVVMAKVSKKNHDFRFDI
                     PTIGVDTIKNLKSIQAKGLVLESNKIFVIDRDKVKKLANQAELSIISITTEEV"
     gene            complement(5175..5978)
                     /gene="lpxA"
                     /locus_tag="VKN64_11360"
     CDS             complement(5175..5978)
                     /gene="lpxA"
                     /locus_tag="VKN64_11360"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMA60874.1"
                     /translation="MRSGEQNNKVLNISDKYQRSYIHPNAKIGKNVEIGPFSTIGPYV
                     EIGDNTKIGSHVVIKGETTIGLSNKIYHGASLGVAVEDNKNDKNLFIGDNNIIREFVK
                     IESGSSRGNGETKIGNNNMIMSYCYISTNCNLSNHIIMTNASSLGEEVLIEDHAVIAG
                     MTTIKDYIRIGRVAMVGGHSNVLKDVPPYVIVDGHPAHVSGINIIGLRRNKIKPSVRK
                     EIKKAYKILYRSDYSIKDAINKMDNQLSEVQEIEHFLRFLRNVQRGICQ"
     gene            complement(6000..6425)
                     /gene="fabZ"
                     /locus_tag="VKN64_11365"
     CDS             complement(6000..6425)
                     /gene="fabZ"
                     /locus_tag="VKN64_11365"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HMA60875.1"
                     /translation="MSSPEEILEVLPHRYPMLLVDKIVEIEPKEKIVAVKNVTFNENF
                     FQGHYPEKPIMPGVLIIEAMAQASGCLLLKSIEKEEKEMYYFAGIDKAKFRKTVIPGD
                     QITLKCEVLKFKKRVAKVKGEAYVGDDLVAESRLLFAMK"
     gene            complement(6452..7297)
                     /gene="lpxC"
                     /locus_tag="VKN64_11370"
     CDS             complement(6452..7297)
                     /gene="lpxC"
                     /locus_tag="VKN64_11370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HMA60876.1"
                     /translation="MKKLTGQSRQQNTINKKVSIKGIGLHTGKKVNMKLLPAPVNTGI
                     IFKRTDLNNTPSIEATIANVSSTERSTNLGQENISVFNVEHFMAALRAYNIDNIKIEL
                     NNKELPALDGSAKVYLDLLEKAGIKEQDETRRIVKIKENYFIKSKNKYLGIFPSDKLK
                     INYLIKFDHPVVQTEYFEFEYTPKSFREEIIPARSFGFQNEIEELKRRGLGLGGSLDN
                     VILLKEDKVVNKLRFENEFVRHKILDLVGDLYLNGPIIGEIIAIRTGHSENYQISQLI
                     QEGMN"
     gene            complement(7310..8350)
                     /gene="lpxD"
                     /locus_tag="VKN64_11375"
     CDS             complement(7310..8350)
                     /gene="lpxD"
                     /locus_tag="VKN64_11375"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMA60877.1"
                     /translation="MNLNNKKEYTLKEILRSIEGTFKGLFSKTHIFTGISTIEDNKAD
                     KITFAEDKNNIQKAEKEAEGIVIVGKDDESSIKNKLEVENVRLTYAKIAELFNPLPYY
                     NPGVASTAVIEDNVKIGKGISINHNTYIGENTEINDNVIIGPGVTIGKNVKIGSNTII
                     HPNVVIERDTIIGKDVTIEASSAIGSEGYGYVTVKGQHHHIPQLGNVIIEDEVEIGAS
                     VTIDRATQSSTIIGKGTKIDNQVQIGHNVEIGKNCLIIAHVGIGGSTTIGDNVILAGR
                     VGVIDHAVIGDNVVVGAASVVTSDIPEGKFYLGNPAQDKMKEMRMRAVRNRLPEYSKL
                     LKKLKQNEETDK"
     gene            complement(8337..8783)
                     /locus_tag="VKN64_11380"
     CDS             complement(8337..8783)
                     /locus_tag="VKN64_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMA60878.1"
                     /translation="MYKKISIILALIIIALVAYFNFYTTEKKIGVIDMNQLLAESQRA
                     DELQSNLQERGKELEKKYENLNSEDVDLEKKENQISMEYAQAKQEIEKQLNNEIDQVI
                     NELNQNNEYQVILYKNKVYYGGEDITDRVIKLLDEKYSEVDENESE"
     gene            complement(8784..9743)
                     /locus_tag="VKN64_11385"
     CDS             complement(8784..9743)
                     /locus_tag="VKN64_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60879.1"
                     /translation="MLREIISGLLIVLILITGFFAYIILFDVKEIKSPSMTLNKEQYN
                     IETDINSNNIKQNLLSPFIESVQAQEDWLEFHQKADQEYKNILSKVGVNQDLLFIEEY
                     KDDLEHKREQLDRDFQEYKEYINNITKKEIEYYSSLVEYEMEQSLENLRKKYDEEFAE
                     FEKQVQAANRNELLNYRLKLETLDLKEAEAENYRNKIEEIESEKSIKINNKLNEIYYT
                     LRIKREELEKEKEEKISKLEKEFIRGKENQIEQKKLELENVLTLFQKNRYAALKKQMS
                     EFKAVLNQESSDLFQKRNAIQEIVQNDLESLKKDYIDSTEERN"
     gene            complement(9767..10231)
                     /locus_tag="VKN64_11390"
     CDS             complement(9767..10231)
                     /locus_tag="VKN64_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMA60880.1"
                     /translation="MKDKKKLIIIIIAMVVVVSSLLLLYQYVNFEGEENKLKDKIGYV
                     NIQTLFNSHPLKKEAENTLNNLARDMQLELENKLEDTPKEEHQQLIDEYQKKLSKKEN
                     ELIDNIVVDIEKKIRKIAVEKEMKVVVKEQDVIKGGYDITQLVLQRIEKRQE"
     gene            complement(10255..11910)
                     /locus_tag="VKN64_11395"
     CDS             complement(10255..11910)
                     /locus_tag="VKN64_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMA60881.1"
                     /translation="MKKRFILLVLSVLIITVMASTVISAQSQDQSITAIEIQGNENIM
                     RSEILSEVETKVGDSFNSEQIKSDMQSIYDLGYFQDVKVNFQNFEGGLKVIFNVVENP
                     KIQEINIEGLEVYDKAQIIDWLGVREGSILNVNKLNSGLKNVITNYQDNGYLVVNITD
                     VNISEEGILNIVLDLGHINEIKLDGNKKTKDYVIMREIALQKGDVLNINDVREASRKI
                     YRLNYFTDISPELERIGEDSNEVNVIFKLTEKKTGNFNFGGGYSSRDGWFGFVKVNES
                     NLGGNGQTLGFNYQFGRNDYYNLNFFEPYFMGYPTSLGLNIYNKYTDRDDYTERSIGG
                     SISVGHDIYRGWRGTARYKIENTTINAISSEYEDSSTELRSITFSARKDTTNYPYNPT
                     SGRNDNLSVEFAGGLLGGDANFTKYNFDLRRYYGGFKEDHSWALRLKGGASTGILPLS
                     EKYNLGGSDSLRGYSLYSFTGDHSLLTQVEYRMQFMDNLTGVIFVDGGQTWDKDETIV
                     LDDLNYGYGLGVRINTAIGQIRLDYGWNEDGIGKTHFSLGNTF"
     gene            complement(11927..16291)
                     /locus_tag="VKN64_11400"
     CDS             complement(11927..16291)
                     /locus_tag="VKN64_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMA60882.1"
                     /translation="MDYRNILIITLIILILAAGYFFYKDIELDPHNAIVSYIEQSTGY
                     EIKYSSAKLWPLNELTIEELNLVGDNFILNVPRVNLGYSIFDYFQNQENVPEIIKYIN
                     LESPEIRYIISEDREENQSSFKDIKESIFNQTDELYINIKEGDILIKQSNTEYRLGSL
                     DTEIKINSEQNNIKLDIKKGLQTSGLVLDKYQLQNYTTTDFRISAELNNESWDLYLKN
                     DNLKLSEFNNLLKDNLGEQFAQYQLNILEGKAQIDFHINGTNENIEDYQANIKLADTV
                     FDFKLKDSSYQEKVAISAGSFHLSSNQNELLVESLNFNFNDVNYNFKGLYNLADNNYS
                     GKLSSKNFQIDSDYLNRFVQADLPFDFATEGSFNVNIDGNLDNVNLISNLKLNNLLIK
                     DQEFVDISAKVRYLDNTLYLDNLYLKTINEAVLEGKGLYDFSKKNYKLDLNGNNVRLA
                     TYLNQEFIQEFVNQYNLTEYLAGKLDFDLSTTGSGRIEDNIVKADFDFEPNRENILRE
                     NGIRLLSGNLLYENNKLFLEKGNLFVNGDRLNLLGQFNLNNQDIYAKLRGEGIGVASL
                     NHYFDLQIKEENKVDISTTIKGKIDEPLIKGEIKSSELAYQNYKVDDVTLDFAYSSRK
                     LTIENVNFVFDEINFNGQGTLSIGKEYKLSQAIVDFSLEAEEINYKKAAQYFEFELPV
                     KGDLEPSIDISGKLSNIEAAGYFISSNTEVNYDGKTYKFEHVEATVTGSLEKNRLELV
                     KGVIRKDDLNIQLKGTYQDNNIDFNFAADNFALEELQLIEEASGIFDIEGKITGGINN
                     PQVSFNFISNNFRYNRFLAEEFTGQVTYNNDTIKFKDVKLKRRSSNYILTGSINDITE
                     NGKQTVDIEISTDRGNTREIINFMDYSSPYSINYPFSGSVNLTGNLTEPTFVLDLSVI
                     NNFVDIIEIKGKISNKIDLNLTGDKVPLDLIKSADLINTDLDFDGNLNFKGTVKGNRE
                     EFVIDLDTNLTDLEVLNIKINDINGNLSYNSSGRFTFKQILNQTDDQNITAEGNLRIT
                     ERFIPEIEIEINNYKLNQIANIDGSISTLKGAIDGEVTLRGEMQNPQLSGNVGVDLSR
                     LAVEGIADITNINGNLSFDERKITLNDIQGKFGQGNFNLTGNINYLNQENFWEVRLQG
                     ENFQFNRGSFNGYYNPDIRILNEFSSPLIFGDLRVFDFVVDSELNWPTSQSEETTEPL
                     FTPDLKLNLIPGENVYFRSENIDIQVEGGSLQLNYQNEELTFIGRLSSNQGALDYYNN
                     KFIVDNVTATFEKYSDNIPVIHLVGSTIAGGTRVFIYVDGPADNLNISFGSQPELPQD
                     KIIALLTRRGGLRGFTSEDDFEPGSLVESELFRYVGEQVQLNFVQRVERSLANIFALD
                     RFEIDTYSLAGEQEVTVYLGKDLTDKLYLQYTGTFSPEIRDSELTFEYDINKYLNLEG
                     GWYGEDDYRFLLETTIEF"
     gene            complement(16343..>17419)
                     /locus_tag="VKN64_11405"
     CDS             complement(16343..>17419)
                     /locus_tag="VKN64_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60883.1"
                     /translation="YIDAIIPSRTQPFKVGSPLDELIGVIEDDRNAGIFGRLNKFPNI
                     IPLTVKVEDKNLNQTKEVRTQIINDEKMLDELGSSIILQSIDSTLDRIGYGTSNIKIT
                     IMGSNLPDGKIVKENMYYSNNDIAVTTLNDYFRMLDMITRNPFKEINIFDMKVDVEIS
                     KDHDIALIQEAEVLNKEVYPGSELDVKVVLRPYRADSISRELSIKIPEDINTGMATLA
                     ISGGFGGLLNRRDPQEDNGNDLNHTVIEGYKSFDEMIKDYLEAPMNNELTLQVYPSLI
                     SRREISNEQPAGEEEQEEQDTEEEESEESVSPQPPEKPEEKPEIKEQVYTDYVLEGNL
                     TLDFEVKKIPEKNEEKNIPEDKKE"
BASE COUNT         5224 a         3381 c         2139 g         6675 t
ORIGIN      
        1 ctttcattcc aatcctatca ccaatatagg cgattatacc gctgaatatt aaaactaaaa
       61 tcattatatt aattccgctc ataatttaca ctcctgttat ctagcaactc tatataataa
      121 aacactacct actattaaaa aaattatatt tgacagccag gccccaagcc agggtgcaac
      181 agttccctgg gaacccaaag catctccaat tgtcatcata atataatata taaagattac
      241 tattatactt attcccattc ccacggctga accgctcgat ctacctggct ttataccgag
      301 aggagccgcc agtagggcaa aaataaaact agcaaaagga atagataacc tttgatgaag
      361 ttttaccttt tcttctaaaa cttcgcgtcc ttgactttct ttgagattaa tgtactcctg
      421 taactcatta aaattcatgt cttccacttt tttatttaat ttcgaaattt cttgcggtct
      481 attaaaaatt tgcacagcat tatattcgtc aaattctaaa gcaggtattc tctcaccagt
      541 tcttaaataa aatattttcc cattgaaaaa cttccaacct gtctccagcc attcagcacg
      601 ttccgcctgt attaaaactg tgggggcacc ttcttcataa tcctgcagat aaacatcatg
      661 cattatgcct gtatctccat caaattgatg tgtgtataaa atataatccg gtctattagt
      721 ttgatcatct agaggtgtca aataaagatt atactgagtg ttaggtaact tttctccatg
      781 tttaaattcc cagacaattt tatcatataa ataattcgac tgaggaacta catattcatt
      841 taaaccgata gcaaaaaaac tcattatcaa acctaatact actgccggta taactaatct
      901 ataaatacta actccaccag ccctaaaagc tgttatctca ctatcattcg aaagtcgact
      961 ataagccata ataattgcta acaaggtagc catcggaaag gttaagacaa ttatagaagg
     1021 taaacttaaa aaaaacaatt ctattaatgt tattatactt atcccccaat tggaataaaa
     1081 atcggttaat ctatataata aatctgtacc gataaagata cctacaaatg ctgctacacc
     1141 aaagaaaaag ggcattatta attcttttgc tatatattta taaactaact tcaaaagttt
     1201 acctccctgt aaagtacaat tagctaccag acagtttttc aatcataatt atattatttt
     1261 tattatttca tttaaaaaat attatatttg aaactcatct ccgagataaa actctctcgc
     1321 taattcactc tctgcaatct cttcagaatt cccggacatc agtatatctc cctcatacat
     1381 gatgtaggct ctatcggtaa tacttaaggt ttctcttaca ctgtgatctg taattaatac
     1441 tcccagacct ttttctttta aatacataat aattttctga atttcactta ccgctatcgg
     1501 gtcaatacct gtaaagggct catctaaaag aataaaagaa ggttcaattg ccaatgatct
     1561 tgctatttct acccttcttc tttctccacc tgacaattga tatccataac tatctcttat
     1621 tttatttaaa tgaaattcat ctaaaagttc ttctatccgg cttttcattt cttcatcagt
     1681 taagtctctt aattctaaaa ctgcctttat attgttatat acactcattt ttctaaaaac
     1741 agaaggttcc tgtgataaat atcctatccc tttttttgct ctctgataca taggttgatg
     1801 agtcaaatct tcctcatcaa gatggacatt accatggtct ggcttaatta aacccacaat
     1861 catataaaaa gtagtggttt taccagcccc atttggtccc aataagccta caatttctcc
     1921 tttatttaca cgaagattaa ctttattaac aactcttcgg ttgtcatatt tttttactaa
     1981 ttctttcgca gtaattgtca tctatttttt actcctcaga aattatattt agttgggcat
     2041 taccctgcat ttctatcctg ttacctctat cgaatatctc tatctgttct gcctgaaatt
     2101 cattgccatt ctgagtgcct gatacattac ccgttagtac ggctctatct tcattttcat
     2161 aaataactat cttatcggca tcagctttga ggttctcgta ctcaatatga gccttccctg
     2221 ttaattcaat tatattatta tttctattat aactaagctt gttactatta cctttaatat
     2281 cttctgcact tatttgaaca ttttcattca tatttaatct ctcttcattt agattatatt
     2341 ttaggtgatt accctttaga tttttatttt ccatagtaat attaacatta ccgtcaaaat
     2401 taattaactc ttctgcctgt ttatataaag cataatctcc tgtaattgta atttcattat
     2461 aaatcaactt cacattatta ttcgcttcta ttatattggt accttcttca ctttttttaa
     2521 atgttaactt atcggcggta agatttttct gagctaaaac attaatatta aataaaaata
     2581 atataataat aaatgaaatt attgcagtct ttttttgaaa caatttattc atggatcttt
     2641 acctccattc aactttcgcc ttggcatcct catttcctat aaattctatc ttatttaaag
     2701 aaagaggggc tgaaaatttt tcagatgtga tgacaaatcg agaagtttca aaccttactc
     2761 caccttctcc tttaattata tcattttttc tattccaaac tatttggtta gtttgtaaaa
     2821 tcgaatcatc cctatttata tttatatcac cagtaaaact aaaaagttcc ttatttttat
     2881 tgtagtttgc cttatctccc cgaccagtat aaattaactc ttcattttca aaggctgtaa
     2941 actcgaggat atccatttta tagatacttc cattgttttc tcttgttagt ttttcactat
     3001 taacattcca tttgatagtc ttatcattat taaataattc caagtcaatc tgggttaaat
     3061 attcttgctg atcttgctgc tccctctcaa ctacagtttc ttcatccttg ttgatttcat
     3121 aatatatagt taaggaaata cctgctatca ggattataat catgagaaat ataagtatga
     3181 gtttcctttt attttttaag tacatctata tttccttccc tcattactaa acgcgcagtc
     3241 ttatctatag ccccttgtga gcctaatttc tctgctgcag aaactaattt tatcttaata
     3301 tcggtcatta aatatggttt agaaagaata taatgggctt cttcatatat tttttcaggg
     3361 gttacttctt cctgcattaa ttcaggcaca atcttttttt ctgctataat attaggcata
     3421 ctgaaaaatt cctgattact taagtattta aacatcttgt atgttacatc actgaattta
     3481 taaataataa ccatcggagt attcaaaatc atcgcttcta aagtagcagt acctgtagaa
     3541 actattgcaa aatgactcat ctttaataat ttatgggtat ggttatttac tattttaact
     3601 tttaagggat aatctataac atttttttca ataaaatcat taaactttgg atctgcagat
     3661 ggaattataa atacataatc tttattatct tctcttaatt taacagctgt ttttaacatt
     3721 attggtaaga gtttttctaa ttctgctttt cggctacctg gtaaaagggc aataggtatc
     3781 tcattttctt taagttcaaa agtgtttttt aattcatcct tttcagcttc aacattaatt
     3841 tcatctaaaa gagggtgacc aacaaacttt gtctcagcct tatttcttct atagacatcc
     3901 cggtccatag gaaaattggc tgcaactact ggattatacc ttgctaaaaa tttggctctc
     3961 cattctcccc acagccaagc tgaaggtgga aaatagttga ctacaggtat atccatctta
     4021 tgacctaatc tagtcataaa catattaaag ccagaataat ccactagaaa aagtacatct
     4081 ggatctcttt ttttagcaat atccttcata accttataat gttctttatg tatttggtag
     4141 tttttaataa cttcccagat cccaactgat tctaattcag taggatctat tactacttct
     4201 acaccctgtt cttttaattt ggttgtaccc ataccgaaaa aatttacatg aggattaagt
     4261 tcctgaattt tttgcactac ttcagctgcc ctttcatccc cggaaatttc accggcaatt
     4321 accatgattt taccggtcat tttagacctc ctcggtagta atagatatta tagataattc
     4381 agcttgatta gctaattttt ttactttatc cctgtcaata acaaaaatct tgttactttc
     4441 taaaaccagg cccttggcct gaatggattt taaatttttt atagtatcca cacctattgt
     4501 ggggatatca aagcgaaaat catgattttt tttacttact ttcgccataa ctactccttc
     4561 tttcaccaaa ttaccggccc gttttatagc ttgatcagtt ccttcaatag cttctacagc
     4621 tactactgct ttattattag tcaaaacagt ttgcccgata ttatagtttg caatctgttt
     4681 agcttttttg aaagcatatt tcatttcctc taataaacta tcatcaggtt ttatattatt
     4741 gaactgccct gcggaaacta ataagtcatc cataaaaaga ttttgtttta ataccttgat
     4801 tccttctttt ttaaactcag caagtaactt ctctaaaata ctattatctt ctctattatc
     4861 agtgtcattt attaacttta gaaaacgctg atcaaattcc tttttttcgt atagatgttt
     4921 cttgtttact ttaccaatca ttattatatt ttctatatta tattttttta atttgtttaa
     4981 caatgcattg tactgtgtaa cactaatata atcaattgta tctgaataat ctcttagttc
     5041 ttccgaatat tcttctttta tgggaaaggc gtgaattttt accccatgtt cttttgcctt
     5101 tttagcccat aaaataggta gttttccgta gccagctatc aatcctatat tattcaaacc
     5161 ggatatactc ctttctattg acaaatgccc ctttgtacat ttcttaaaaa tcttaaaaag
     5221 tgttctatct cctgtacttc acttaattga ttatccattt tattaatggc gtcttttata
     5281 ctataatctg atctataaag aattttatag gcttttttaa tctctttacg gaccgaaggc
     5341 ttaattttgt tgcgccttaa tcctataata tttatacctg aaacatgggc aggatgaccg
     5401 tcaactatta catagggagg aacatctttt aatacattgg aatgtccacc gaccattgca
     5461 actcttccta tccttatata atccttaata gtagtcatac ctgcaatcac ggcgtgatct
     5521 tcaattaaaa cctcttcccc taaagaagat gcattggtca taataatatg attggataga
     5581 ttacagttag tggaaatata acagtaagac ataatcatat tattattacc tatctttgtt
     5641 tctccatttc cccgggaaga gccgctttca atcttcacaa attcacggat tatattatta
     5701 tcccctataa aaagattctt atcattttta ttatcttcaa cagcaactcc tagagaggca
     5761 ccatggtaaa tcttattact taatccaata gtggtctccc cttttatcac tacatgagaa
     5821 cctatttttg tattatctcc gatttccaca tagggtccaa ttgtagaaaa aggacctatt
     5881 tctacatttt tcccaatctt ggcattagga tgaatatatg atctctgata tttatcagaa
     5941 atatttaata ccttattatt ttgttctcct gatctcaata tagatttctc ctttcaatat
     6001 tatttcattg caaaaagcag tctgctttca gcaactaaat catcacccac atatgcttct
     6061 ccttttactt tagccaccct tttcttaaat ttgagtactt cacattttaa agttatttgg
     6121 tcgcctggaa tgactgtttt tctaaattta gctttatcaa tgcccgcaaa ataatacatt
     6181 tctttttctt ctttttcgat gctttttaat aatagacagc cgctcgcttg agccatggct
     6241 tctataatta aaacacctgg catgattggt ttttcaggat aatgaccttg aaaaaagttt
     6301 tcattaaaag taacattttt tactgcaact attttttctt ttggttctat ctccactatt
     6361 ttgtctacta aaagcatggg ataacgatgg ggaagaactt ctaaaatttc ttccgggcta
     6421 gacattttat tcacctcttt ttttaatctt attaattcat accttcctgt attaattggc
     6481 taatctgata attttcagaa tgtcctgttc ttatagctat aatttcacca attattggac
     6541 cattcaaata aagatctcct actaaatcta aaattttgtg tcttacgaat tcattttcaa
     6601 atcttaattt attaactact ttatcttctt ttaataatat tacattatca agactaccac
     6661 ctaatcctaa acctcttctt tttaattctt caatctcatt ttgaaatcca aaagaacgcg
     6721 ccggtattat ttcttccctg aagctttttg gggtatattc aaattcaaaa tactctgttt
     6781 gcactaccgg atggtcaaat ttgattaaat aattaatttt caatttatcg gaaggaaata
     6841 ttcctaagta cttatttttt gatttgataa agtaattttc ttttatctta actattcttc
     6901 tggtttcatc ctgctctttt atacctgctt tttctaataa atctaaataa acttttgcac
     6961 ttccatctaa agctggtagt tctttattgt ttaactcaat tttaatatta tctatattgt
     7021 atgctcttaa agcagccata aagtgttcta cattaaaaac actaatattt tcttgaccaa
     7081 ggttagttga cctttcagtg gagctaacat ttgctatagt ggcttcaata ctaggagtat
     7141 tattaagatc agttctctta aaaataatac ccgtatttac aggagcaggt aataacttca
     7201 tattaacttt tttgccggta tgaagcccaa tcccttttat tgaaaccttt ttattgatag
     7261 tattctgctg cctgctttgt ccagtcaact tcttcatcct tcctcttttt tatttatccg
     7321 tttcctcatt ttgctttaat ttttttaaaa gtttactata ttctggtaat ctatttctta
     7381 cagccctcat ccgcatctcc ttcattttat cttgagcggg attaccgaga taaaatttac
     7441 cttccggtat atcagaagtt acaacactgg ctgccccaac taccacatta tcgccaatta
     7501 cggcatgatc aattactcca acgcgaccgg ctaagataac attatcgcct atagttgtac
     7561 tgcctcctat acctacatgg gctataatta aacagttttt gcctatttct acattatgtc
     7621 caatctggac ctgattatca attttagtac ccttacctat aattgtagaa gattgcgtag
     7681 cccggtcaat tgtaacactc gcccctatct ctacctcgtc ttcaataatt acatttccta
     7741 attgaggaat atgatgatgc tgccctttta cagtaacata accataaccc tcagaaccta
     7801 ttgctgaaga ggcttcaata gtaacatctt tgccaataat agtgtctctc tctattacta
     7861 cattaggatg aatgatagtg tttgatccta tttttacatt tttaccaatt gttacaccgg
     7921 gaccgataat tacattatca tttatctcag tattctcacc tatatatgta ttatgattaa
     7981 ttgatatacc cttacctatt ttaacattat cttctatcac agctgtagaa gctacccccg
     8041 gattatagta aggaagggga ttgaataatt cagctatttt agcataagta agtctaacat
     8101 tttcaacctc tagtttattt tttatactgc tttcatcatc ttttcctact atcactatac
     8161 cttctgcttc tttttctgct ttttgtatat tgtttttatc ttctgcaaaa gtaattttat
     8221 cagccttgtt atcttcaatt gtactaatac ctgtaaagat atgggtcttg gaaaataatc
     8281 ctttaaatgt accttcaata ctccttaata tttcttttaa agtatattct tttttattat
     8341 tcagattcat tttcatcaac ctcagaatat ttttcatcta aaagcttgat tactcggtct
     8401 gtgatatcct ctccaccata atatactttg tttttatata gtattacctg gtactcatta
     8461 ttttgattta attcattaat aacctgatct atttcattat taagctgttt ttctatctcc
     8521 tgtttggcct gtgcatattc catcgaaatt tgattctctt ttttttctaa atccacatct
     8581 tcggaattta aattttcata ctttttctct aattctttac ctctttcttg tagattgctc
     8641 tgcagttcgt ctgctcgctg gctttctgca agcagctggt tcatatcgat taccccaatc
     8701 tttttttcag tagtatagaa attaaaatat gcaactaagg caataatgat tagagctaat
     8761 attatagata tttttttata catttaattt ctctcctcag tagaatcgat atagtctttt
     8821 ttgagacttt ctaaatcatt ttgcacgatc tcttgaatcg cgtttctctt ttgaaataaa
     8881 tcagaacttt cttgattaag tactgcttta aattcactca tctgtttttt taaagcagca
     8941 tatctgttct tttgaaaaag ggttaaaaca ttttcaagtt cgagcttttt ttgttcaatc
     9001 tgattctctt tgccgcgaat aaactctttt tctaacttac ttattttctc ttctttttct
     9061 ttttctaatt cttcccgttt aatacgaagt gtatagtaaa tttcatttaa tttattgttg
     9121 atcttaatac ttttttctga ttcaatttct tcaattttat ttctgtaatt ttcggcttct
     9181 gcttctttta aatccagggt ttctaacttg agtctataat ttaagagttc atttctatta
     9241 gctgcttgga cctgcttttc aaattcagca aattcttcat catatttttt acgtaaattt
     9301 tctaaggact gttccatctc atattcaact aaagaagaat agtattcaat ttctttctta
     9361 gttatattat tgatatattc tttatattct tgaaaatctc tatcaagttg ttctctttta
     9421 tgctctaaat catctttata ttcttcaata aaaagtaggt cctgattgac acccacctta
     9481 cttaatatat ttttatattc ctggtccgct ttttgatgaa attcaagcca atcctcctga
     9541 gcctgaaccg actctataaa tggactcaat aagttttgtt taatattatt actattaata
     9601 tcagtttcaa tattgtactg ttccttattt aaagtcatgg aaggagattt gatctctttt
     9661 acatcaaaga gaattatata agcaaaaaat ccggttatta aaatcaatac aataagtagc
     9721 ccacttatta tttctctcaa catggatcaa acctcccttt attgctttat tcttgtctct
     9781 tttcaatccg ctgcaagaca agttgagtta tgtcataacc acctttaatt acatcctgct
     9841 ctttaacaac aaccttcatt tctttttcaa ctgctatttt ccttatcttt ttttcaatat
     9901 caaccactat attatcaatc agttcatttt ccttttttga taacttcttc tgatattcat
     9961 ctattaactg ttgatgctct tctttggggg tatcttctag cttgttttct aattccaact
    10021 gcatgtcacg ggctagatta tttaaagtat tttctgcttc ttttttaaga ggatggctgt
    10081 taaaaagggt ctggatattt acatagccaa tcttatcctt taacttgttt tcctctcctt
    10141 caaaattcac atactggtag aggagtaata ggctgcttac tactacaacc atcgctatta
    10201 ttataataat tagtttcttt ttatctttca atatttatta ctccttccag gcaattaaaa
    10261 tgtgttacca agactaaagt gggtcttacc aataccgtct tcattccaac cgtaatccaa
    10321 tctaatctga ccgatagctg tattaattct aacacctagg ccatatccat aattaaggtc
    10381 gtcaagaaca atggtctcat ccttatccca ggtttgtcct ccatcaacaa atatgacacc
    10441 tgttagatta tccatgaact gcatcctata ttctacctga gttaagagag aatgatcacc
    10501 tgtaaaggaa tataggctgt aacctctcag tgaatcagaa cctcccagat tatacttttc
    10561 tgaaagcggt aggataccgg ttgaagcccc tccttttaat cttaatgccc aggagtgatc
    10621 ttctttaaat ccaccatagt atcttcttaa atcaaaatta tatttagtga agttagcgtc
    10681 accacctaaa agtcctccgg caaattcaac agataggtta tcatttctac ctgaagtcgg
    10741 gttatagggg taattagttg tatcttttcg agcggaaaat gtaatacttc tcaactctgt
    10801 actgctatct tcatattctg aactaatggc attaatagtt gtattttcga ttttatatct
    10861 agcggtacca cgccaacctc tatatatatc atgtccaact gatatactac ctccaataga
    10921 tctttctgta taatcatctc tatcagtata tttattataa atatttaatc ctaaagaagt
    10981 tggataaccc atgaagtaag gttcgaaaaa gttcagatta taataatcat ttctaccaaa
    11041 ctgataatta aatcctaaag tttggccgtt acctcccaga tttgattcat tgacctttac
    11101 aaaaccaaac caaccatctc tagaactata accaccaccg aagttaaagt tacctgtctt
    11161 tttctcggtt agcttaaata ttacattgac ttcattactg tcttctccaa ttctttctaa
    11221 ttccggacta atatctgtaa agtaattcaa ccgataaatt tttcgggatg cttctctgac
    11281 atcattaata tttaacacat cacctttttg taaggctatt tctctcatga tgacataatc
    11341 tttagtcttt ttattgccat ctaatttgat ttcattaata tgacctaaat ctaatactat
    11401 atttaatatc ccttcctcag aaatattaac atcagtaatg ttaactacca ggtaaccgtt
    11461 atcctgataa ttagtaatta cattctttaa accagaattc aacttattta catttagaat
    11521 actgccttct cttacaccaa gccagtcaat aatctgtgcc ttatcatata cttctaatcc
    11581 ttcgatattg atctcttgaa tcttggggtt ttctaccaca ttaaatataa ctttgagacc
    11641 accttcaaaa ttttggaaat taactttgac atcttgaaaa tatcccaaat cataaattga
    11701 ctgcatatca ctttttattt gttcactatt aaatgaatcg ccaactttag tttctacctc
    11761 tgataaaatt tcacttctca ttatattttc attaccctgt atttctatag ctgtgattga
    11821 ctgatcctgc gactgtgcag atatgactgt agaagccata actgtaatta ttaatacaga
    11881 taatactaat aagataaatc tctttttcat tattttcctc cttcatttaa aattcgattg
    11941 ttgtttctag taaaaatcta taatcatctt ccccgtacca gccaccttct aagttaagat
    12001 atttattaat atcatattcg aaagttaact cactatccct tatttccggg gagaatgttc
    12061 cggtatactg tagatatagt ttatctgtta aatctttgcc taaataaact gtaacttctt
    12121 gttcaccagc taatgaatag gtgtctattt caaagcggtc cagagcaaaa atatttgcca
    12181 gacttctttc cactctttgc acaaagttta attggacctg ttcacccacg tatctaaata
    12241 attctgattc aactagtgaa ccgggttcaa aatcgtcttc tgaagtaaaa cctcttaatc
    12301 cgcctcttct agttagaagg gcaataatct tatcctgagg aagttcaggt tgagatccaa
    12361 aactgatatt caagttatca gccggaccgt ctacatagat aaataccctt gttccgcctg
    12421 ctattgtact accaactaaa tgaattacgg gaatattatc actatatttt tcaaaagtag
    12481 ctgtcacatt atcaacaata aatttattat tatagtaatc aagtgcacct tgattacttg
    12541 ataatctccc aataaacgtt agttcctcat tttgatagtt tagctgtaag ctaccccctt
    12601 ccacctgaat atcaatattc tcactccgga aatagacatt ttctcccgga atcaagttta
    12661 atttcaaatc aggagtaaat aatggttcgg tagtctcttc cgattgggaa gtaggccagt
    12721 ttaattcact gtctactaca aaatcaaata cccttaaatc gccaaatatt aaaggagaac
    12781 tgaattcatt taaaattctt atatctggat tataatatcc attaaaagaa cccctgttaa
    12841 attggaagtt ctctccctgt aaccttactt cccagaaatt ttcctgattt aagtagttaa
    12901 tgttaccagt taaattaaag ttgccttgcc caaacttacc ttgaatatca tttaaagtga
    12961 tctttctttc atcaaaggat aaattaccat ttatattagt tatatcagca attccctcca
    13021 cggcaagcct agataaatct acacctacat ttccacttaa ttgtgggttt tgcatttcgc
    13081 ctcttaaggt aacttctcca tctattgccc ctttaagtgt gctaatgctc ccatcaatgt
    13141 tagctatctg atttaattta taattgttaa tctctatttc aatttcgggt ataaatcttt
    13201 ctgtaattct aagatttcct tcagccgtga tattttggtc atctgtttgg tttaatatct
    13261 gtttaaaagt aaatctccca gaactattat aacttaaatt cccattaata tcattgatct
    13321 tgatatttaa aacttctaag tctgttaaat tggtatctag atcaattaca aattcttctc
    13381 tattaccttt aaccgtccct ttaaaattca aattaccatc gaaatccaga tcagtattta
    13441 taagatcggc agatttaatt agatcaagag gcaccttatc tccggttaga tttaaatcta
    13501 tcttgttaga tatcttaccc ttaatttcaa taatatcaac aaaattgttg attactgaaa
    13561 gatctaatac aaaagtgggc tcggttaaat taccggtcag attaactgac ccgctaaaag
    13621 gatagttaat actatacgga ctactatagt ccataaagtt tattatctct ctggtattac
    13681 cacggtctgt acttatttca atatctaccg tctgctttcc attttctgtt atgtcattaa
    13741 tactccctgt taagatataa tttgaagatc tcctttttaa ttttacatct ttaaatttaa
    13801 ttgtatcatt attataggta acttgaccag tgaattcttc cgctaagaac ctattatatc
    13861 taaaattatt agatataaaa ttaaatgata cctgcggatt attgatacca ccggtaattt
    13921 taccttcaat atcgaaaata ccggaagctt cttctattaa ctgcagttcc tctaaggcaa
    13981 agttgtcggc agcaaaattg aaatcaatat tattatcctg ataagttcct tttagttgaa
    14041 tatttaagtc atcttttcta attacacctt taactaattc taagcggttt ttttctaaac
    14101 tgccggttac agtagcctca acatgttcaa atttatatgt tttaccatca tagttaactt
    14161 ctgtattact ggaaataaaa tatccggccg cttcaatatt agataactta ccagaaatat
    14221 caatacttgg ctcaaggtca cctttgaccg ggagttcaaa ttcaaagtac tgggccgctt
    14281 ttttataatt aatttcttca gcttctaaag aaaaatcgac aattgcctgg gataatttat
    14341 actcttttcc tatacttaac gtaccctgac cattgaaatt aatctcatcg aaaacaaagt
    14401 ttacattctc tattgttaac tttcgcgatg aatatgcgaa atctaaagtt acatcatcta
    14461 ctttataatt ttgataagct aattcactac ttttaatttc acccttgatt aaaggttcat
    14521 cgatcttacc tttgattgta gtgctaatat caactttatt ttcttccttg atttgtaggt
    14581 caaagtagtg attaagagag gcaacaccaa taccttcccc cctgagctta gcgtaaatgt
    14641 cctgattgtt taaattaaat tggccaagta gatttaacct atcaccgtta acaaataaat
    14701 tacctttttc tagaaacaat ttgttgtttt catataaaag gttaccactt agtaagcgta
    14761 taccattttc tcttagaata ttttctctat ttggttcaaa atcaaaatca gctttcacta
    14821 tattatcttc tatccgacca ctaccagtag tggaaagatc aaaatcaagc ttaccagcta
    14881 gatattctgt taaattatat tgattaacaa attcttgtat aaactcctgg ttcagataag
    14941 tcgccagcct tacattattt ccattaagat ctaacttata atttttctta ctaaaatcat
    15001 agaggccttt accttctaat actgcctcat tgattgtctt taaatacagg ttatctaaat
    15061 ataaagtatt atctagataa cggactttag ctgaaatatc tacaaattcc tggtctttaa
    15121 tcagtaaatt atttaattta aggttggaaa ttagattaac attatctaag ttaccatcaa
    15181 tattaacatt aaaactacct tcggttgcaa agtcaaaggg taaatcagct tggacaaatc
    15241 tgttaagata atcactatct atttgaaaat ttttgctgct taatttgccg gaataattat
    15301 tgtcagctaa attatataaa cccttaaaat tataatttac atcattaaag ttgaaattta
    15361 aggattcgac caataattcg ttttgatttg aacttaagtg aaaagacccg gctgaaattg
    15421 caaccttttc ctgataggaa ctgtctttta atttaaaatc aaaaaccgta tcggctaatt
    15481 taatattggc ttgataatct tcaatatttt catttgtccc attaatgtga aaatcaattt
    15541 gggctttacc ttctaaaata ttgagctggt attgagcaaa ctgttcacct aagttatctt
    15601 tcagtaaatt attaaattca ctcaacttta aattatcatt ttttaaatat aaatcccagc
    15661 tttcattatt aagttcagct gagattctaa aatcggttgt agtataattt tgaagttggt
    15721 atttgtctaa aacaagtcca ctggtttgta aacctttttt aatatccagt tttatattat
    15781 tttgctcact gtttatctta atctccgtat ctaaagaacc taatcggtat tctgtattgg
    15841 actgtttaat taaaatatct ccttctttga tatttatata taattcatca gtctggttaa
    15901 aaatagattc tttaatatct ttaaaagaag actgattttc ctctctgtcc tctgaaataa
    15961 tatatcttat ttccggactt tctaaattta tatatttaat aatttccggc acattttctt
    16021 gattttgaaa gtaatcaaaa atggaatatc ccaggtttac cctaggcaca tttaggataa
    16081 aattatcacc aactaaattg agctcctcaa tagttagctc gttaagaggc cataatttag
    16141 ctgatgaata tttaatttca tacccggttg attgttcaat atagcttaca attgcattat
    16201 gaggatctaa ttcaatatct ttatagaaaa aatatcccgc agctagaatc aagattatta
    16261 aagttattat taaaatattt ctgtaatcca tatttatcct ccagctttct ataaaaaatt
    16321 taaagccgtt ttattatctt aattattctt ttttatcttc cgggatattt ttctcttcat
    16381 tcttctctgg gatctttttc acctcaaaat caagggttaa atttccttcc agaacataat
    16441 ctgtataaac ctgttctttt atttctggtt tttcttcggg cttttccgga ggctgaggac
    16501 ttacgctttc ctcagactct tcttcttctg tatcttgttc ttcttgctct tcttccccag
    16561 caggctgctc attactaatt tcccggcggg aaatcaggga aggataaacc tgtaatgtta
    16621 actcattatt catgggtgct tcaagataat ctttgatcat ttcatcaaag cttttatatc
    16681 cttcgattac agtatggttt aaatcattac cattatcttc ctgcggatct cgcctgttta
    16741 gaagacctcc aaagcctcca ctgattgcca gggttgccat accggtatta atatcttccg
    16801 gtatcttaat tgaaagctct cggctaatag aatccgctcg atatggtctt aaaactactt
    16861 ttacatctaa ttcgctaccc ggatatactt ctttatttag tacttctgct tcctgtatta
    16921 aggctatatc atggtctttg ctaatttcta catctacctt catatcgaat atatttattt
    16981 ctttaaaagg atttctggta atcatatcca gcatcctaaa gtaatcattt aaagtagtaa
    17041 ctgcaatatc attgttactg taatacatgt tttccttgac gatctttcca tctggtaaat
    17101 tggaacccat gatagttatc ttaatattag aagttccata acctattcta tctaaagtac
    17161 tatcaattga ttgtaaaatt atgctgctgc ccagctcatc aagcatcttt tcatcattaa
    17221 taatctgggt cctaacttct tttgtctgat ttaaattttt gtcttccact ttcacagtta
    17281 aaggtattat attgggaaat ttgtttaatc tcccgaaaat accggcattc cgatcgtctt
    17341 ctataacacc aataagttca tctagtgggc ttcccacctt aaagggctga gtccgactgg
    17401 gaatgatagc atcaatata
//
LOCUS       DATGCJ010000259         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_265850, whole genome shotgun sequence.
ACCESSION   DATGCJ010000259 DATGCJ010000000
VERSION     DATGCJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265850"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2901
                     /locus_tag="VKN64_11410"
     CDS             <1..>2901
                     /locus_tag="VKN64_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMA60884.1"
                     /translation="TNTGNIADYINLELDINQDWNYNIYLDLNNNKIVDEEDSFLIDN
                     NGDSYFDTGLIEPGDKVHILIQVTPDQSLLNGLKQDINVNVFSSINSEIKDEATNVVS
                     INNNVPYIEIRQSPETVYGGQSYKYTIFYKNQGENTLTNVVIENILPPELVYISSEGN
                     GNFISDGTTKKINWTVDELLAGESGSFEVTVNAVNKNNVIYTVINEATLYSDQTEIIS
                     DKTTTEIVPVTPNKLTIDVNPKIIKGDGESTALFTVNVFDILGRKVPDGTKVTFETEA
                     GIFLESGNKYYIGYTEDGAVTAELLSPVLYGDEEINNLVTVTASDKEGNEVSESVIIT
                     FIPGGIAGEIINEKYEFPESGVKVSLMDKDGTYLIDVANNPTYTNSDGRYFLTAPEVN
                     EYLVIFDIPEVGEVKELVNVTELEGAVFYPSELLSGGVRFGQNQNILLLQNIRPLENL
                     KVSLYDISDSYLEEPLKTTYTDQNGNYIFSDLNIESFPKRYIITAEKEINGISYKSRE
                     TITFTSRGQFRINKILNIFSTGKVLDEKTQEPIESAEVTLVYAEGNNVGETVELPNQG
                     NPIYTNSQGEYIIFPPAGSYKIRVSAGGYIEYQSDKIVTSGEPITNIINLSSETIGGI
                     NLSKIADKNEVRPNEKINYQISFENTKQVTIENLILKDELPYNITIEPDNLPSGLVYD
                     SDNHRLSFEIGDLESGQGQEFNFTATVKEETADGTKLNNRVYVEDKVSHSVYALANAE
                     SSVIRKAEISISHFADEASYKVGEIVNYTIKINNEPDQEFQYTIYDLIISGVLPPGFK
                     YIENSSVINGEKVDDPSIMDNTLSWNHTSLSSGDELEIKYKAKITPAGKAKQAESKAY
                     LEAKTSQATEFTEGPAISIVNVLGPMFTEKAGIFGRIFIDSNQDGLQTEGEKVIEKAK
                     IILNNGTIITTDQNGDYSHNLSPGTYLMAIAPESLYRINRHLDKKGLEIIDK"
BASE COUNT         1110 a          389 c          540 g          862 t
ORIGIN      
        1 aacaaatact ggtaatatag ctgattatat taatcttgaa ttagatataa atcaagactg
       61 gaattataat atttatcttg accttaataa taataaaatt gttgatgaag aagatagttt
      121 tcttatagac aataatgggg atagttattt tgacactgga ttaattgagc ctggagataa
      181 ggttcatatt cttatacaag tcactccgga tcaaagttta ttaaatggtt tgaagcaaga
      241 tatcaatgta aatgtttttt catctatcaa ttcagagatc aaggatgagg caactaatgt
      301 ggtctcaatt aataataatg ttccatatat tgagataaga caatcacctg aaactgtata
      361 tggtggacaa agttataaat atactatatt ttataaaaac cagggagaaa atacattaac
      421 taatgttgta atagaaaaca ttttaccccc tgaactagtc tatattagct ctgaaggtaa
      481 tggaaatttt atttcagatg gtacaactaa aaaaattaac tggacagtgg atgaattatt
      541 agctggagaa agtggtagct ttgaagttac agtaaatgct gttaacaaga ataatgtaat
      601 atacacagta ataaacgaag caactctgta ttcagatcaa actgaaataa tctctgacaa
      661 gacaaccaca gagatagttc ctgtgacacc aaataaacta actattgatg ttaatcctaa
      721 aataattaaa ggtgatggtg aatcaacagc actatttact gtgaatgtat ttgatatact
      781 tggcagaaaa gttccagatg gaactaaggt tacatttgaa actgaagcag gaatattttt
      841 agaaagtggc aataaatatt atattggata tacagaagat ggggctgtaa cagcagagtt
      901 attaagtcct gttttatatg gagatgaaga aataaacaat cttgttactg taacagctag
      961 tgataaagaa ggtaatgaag tttcagaatc tgttattatt accttcattc ctggtggaat
     1021 agccggcgag ataattaatg aaaaatatga atttcctgaa agtggagtta aagtatcact
     1081 tatggataag gacggtactt atctaatcga tgtagctaat aatcctactt ataccaattc
     1141 agatggtcgg tactttttaa cagctcctga ggttaatgaa tatctagtta tctttgacat
     1201 tccggaagta ggagaggtaa aagaacttgt aaatgtgact gaactagaag gtgcagtttt
     1261 ttatccttct gaattacttt ctggcggagt aagatttggt cagaatcaga atatattact
     1321 tttacagaat ataagaccgc ttgaaaactt aaaagtttcc ctatatgata tatctgattc
     1381 ttatttagaa gaaccattaa aaacaactta cactgatcaa aatggcaact atattttcag
     1441 tgatctaaat attgaaagtt ttcctaaaag atatataata acagcagaga aagagataaa
     1501 tggaataagc tataaaagca gagaaactat aacctttaca agccggggac agtttaggat
     1561 caataagata ttaaatattt tttctaccgg taaagtattg gatgaaaaaa ctcaagaacc
     1621 gatcgaatct gcggaggtta ccctcgttta cgctgaagga aataatgttg gtgaaactgt
     1681 agaacttcct aatcaaggta atccgatata taccaattca caaggtgaat atattatatt
     1741 tccacctgca ggaagttata aaataagagt ttctgcagga ggatatatag aatatcagtc
     1801 tgataagatt gttacttcag gagaacctat aaccaatatt ataaatctta gttctgaaac
     1861 tatcggaggt attaacttaa gtaaaattgc agataagaat gaggtaaggc caaatgagaa
     1921 gataaactat cagattagtt ttgaaaatac taaacaggta acgattgaaa atttaatatt
     1981 aaaggatgaa cttccttata atattacgat agaaccagac aacttaccgt cgggattagt
     2041 atatgattct gacaaccata gattgagttt tgagatagga gatttagaaa gtggtcaagg
     2101 ccaagaattt aattttacag ctacagtaaa agaagaaaca gcagatggaa cgaagctaaa
     2161 taatagggta tatgttgaag acaaagtaag tcattcagta tatgctcttg ctaacgcgga
     2221 aagtagtgtt ataagaaaag cagagatttc catctctcac tttgcagatg aagcttctta
     2281 taaagtagga gaaatagtaa attacactat aaaaatcaac aatgaaccag accaagaatt
     2341 tcaatatacc atttatgatt tgataatttc cggtgtgttg cctccagggt ttaaatatat
     2401 tgaaaatagc agtgtaataa atggtgaaaa agtggatgat ccctcaatta tggataatac
     2461 attgagctgg aatcatacaa gtttaagttc tggagatgaa cttgaaataa aatataaggc
     2521 aaaaataacg cctgcaggta aagctaagca agcggagagc aaggcctatc tagaagccaa
     2581 aaccagtcag gcaacagagt ttacggaagg tcctgctata agtatagtaa atgtactagg
     2641 tcctatgttt accgaaaaag caggtatatt tggtaggatt tttatcgatt caaatcagga
     2701 tggtctacag acagaaggag aaaaagtaat agaaaaagca aaaataatat tgaataatgg
     2761 tacaattatt actactgatc agaatggtga ttattcccac aacctatctc cgggtacata
     2821 tctgatggct attgcacctg aatctctcta tcgaattaat cgacatttag ataaaaaagg
     2881 acttgagata attgataaat t
//
LOCUS       DATGCJ010000260        12276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_523171, whole genome shotgun sequence.
ACCESSION   DATGCJ010000260 DATGCJ010000000
VERSION     DATGCJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523171"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VKN64_11415"
     CDS             <1..558
                     /locus_tag="VKN64_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60885.1"
                     /translation="SAAGGYGIIAAAKDKDIKCIIGQCPGLDNNADNNDVFKREGIGF
                     FIKLFIHAQRDKGRSRFKLSSHTIPLVGKPHTTAMHTVPGAFQGYSELATDNFKNEVC
                     ARLILMRHLNPIDYADKMDCPALFQICENDQFVSAQSYVKTEEILGKKAKFIKYPIGH
                     FDIYKGENFEKAVNKQIEFLKENFN"
     gene            600..1085
                     /locus_tag="VKN64_11420"
     CDS             600..1085
                     /locus_tag="VKN64_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMA60886.1"
                     /translation="MVDFIIREIINQPRDIVIKAFLNPANMIFWTKDLEKFEVIAGKP
                     GEIGSIAYLHYRQNNKRYKMRDELIECNPGFRYLSRVTGDTITATVETYLKDVGNDTE
                     LTIHWTGSGNSFLLKIILPLSKRKMIHQAKNKLKTFKDLVEKRGVHFNIFVHKISKYT
                     L"
     gene            complement(1094..1294)
                     /locus_tag="VKN64_11425"
     CDS             complement(1094..1294)
                     /locus_tag="VKN64_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMA60887.1"
                     /translation="MPELKTKIKEHRAKIDLTQKELADLVDVRRETIVHLEQGKYNPS
                     LKLAMDIAAVLNVTVEELFKFE"
     gene            complement(1303..1629)
                     /locus_tag="VKN64_11430"
     CDS             complement(1303..1629)
                     /locus_tag="VKN64_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HMA60888.1"
                     /translation="MKTNWIKYLGLMGIVGLLGLFTSNPSLYGFFGFFGFFGFANIKS
                     DERLEENINKAAKNTFITTLIIFILTIVIGSLASNPDLFIYGLIINFVQHILVFTISF
                     HIYDRG"
     gene            1772..2269
                     /locus_tag="VKN64_11435"
     CDS             1772..2269
                     /locus_tag="VKN64_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMA60889.1"
                     /translation="MKNWKTYILSIFWVPLLLMQIVLVIFMGKVNEAGLIWLFYLGWL
                     IWIISIILGWLPIYTLKKRGAVPAGKSYVHTKKLVTSGIYSIVRHPQYTAGMLLSLAL
                     LLISQDLWVIIIGIKVIVLLYIDIYLTDKWEINKFGQEYKKYMKEVPRVNFILGLIRL
                     YKQRR"
     gene            2275..2586
                     /locus_tag="VKN64_11440"
     CDS             2275..2586
                     /locus_tag="VKN64_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60890.1"
                     /translation="MRINIIPRSKYGKWAIYAILAFFFFWALFYLLVEMGERGGATFF
                     SNFKLTIPFLAAAISGIAALVMGLLSIFKSKDHCILVYISIIIGLFVLIFIMGEIIYP
                     H"
     gene            2734..3108
                     /locus_tag="VKN64_11445"
     CDS             2734..3108
                     /locus_tag="VKN64_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMA60891.1"
                     /translation="MPRVVHFDIKTEEPEKLKKFYEKVFNWKFDKWNDPSGQMEYYLV
                     NTGEEEPGIDGGLAKREGPDDQIDITIGVKDIERYAKLIEENGGKILSPKMSISGVGY
                     LMNFEDPQENKYSIMEDDPEVK"
     gene            3128..3904
                     /locus_tag="VKN64_11450"
     CDS             3128..3904
                     /locus_tag="VKN64_11450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60892.1"
                     /translation="MNGFEIIETNEENVMKLGLCGYKNIKREGFPEKIEWLKKQKDLG
                     LTIKSLYSKENKTQGMIEYIPGQYCWRPVKAKDYMFIHCLFVGFKKEYKNKGYGSLMI
                     KECIKDSKKQNFKGIAVVTRKGSFMSGKEIFIKNNFKVVDNAPPDFELLVYKFKEDTL
                     DPKFNNNWSEKQKEYGNGLVIIRANQCPYTVKNVNEIVKTAKQEYKINAKVIDLKNYQ
                     EAQNSPCPFGTFCILFKGKAIANHPISKRRFMNIMSKEVV"
     gene            3905..4522
                     /locus_tag="VKN64_11455"
     CDS             3905..4522
                     /locus_tag="VKN64_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60893.1"
                     /translation="MITELHNKDININKLVAQVNEDESLILELLDNLNSKEETIRYNS
                     YQIIKGISLKYPEKLYSYRDEFGKKLYIDNAYQMLIGIIILAHLCVVDKDHKFEQIFN
                     KYCSLLNDRRITVSLYVLSNLGRIAKVKPNLQEKITIILLDSKNTHHKHKGLIAGAAI
                     ESFEKYYIEIEDKEKVLEFVKEQIVCDSPKTRKSAVEFLTKWDNK"
     gene            4818..5363
                     /locus_tag="VKN64_11460"
     CDS             4818..5363
                     /locus_tag="VKN64_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60894.1"
                     /translation="MEAIEMDRIEKILISKIKPKEKSQKIVDMVIEEKIRTEDFISYF
                     IMTSDVKKGTCADAMKHIAKLRPEILYPYIDLLIEYINYNAPRVKWGIQEAIGNLAER
                     YPDEVAKAIPGLLKNTEETNKNTTVVRWCAAYGLAEILINNKQIQKDLLPKIKEIVST
                     EKNNGVKNVYLKALRRLERIN"
     gene            5428..5622
                     /locus_tag="VKN64_11465"
     CDS             5428..5622
                     /locus_tag="VKN64_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60895.1"
                     /translation="MRKNIFWIFGILQSVSLSVLIYIVLNSLDLIYKGPLVGKDSIFV
                     LSAAFPTFLLIVEYIIYTKE"
     gene            5683..6582
                     /locus_tag="VKN64_11470"
                     /pseudo
     CDS             5683..6582
                     /locus_tag="VKN64_11470"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters.; internal
                     stop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
     gene            6582..7526
                     /locus_tag="VKN64_11475"
     CDS             6582..7526
                     /locus_tag="VKN64_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMA60896.1"
                     /translation="MEINIKEVKEEYKESALNLVLDSYQNLKKEISFLPTKSTYKQKF
                     KDKIEHLFEKGLGVIALQEDKLIGFLIGYEVDELFGKDKGVYVPLYAHAAVNNKKRVY
                     QKMYTRAAELWINRSCVIHALTIFTCDKDVIHAFYWLGFGLRCIDAIQKVDLIEKSSH
                     NKVKIIKAELKEIELMEELYKKDNLYYGSSPLFMPVNQKSNIDKLRKWLNKDNHHLWM
                     ALIEDRVVGYIRIEPTGETVISTYSKMMNITGAFVEEKYRDKRIGKFLLDEVLIYLKK
                     NNYMLCGVDFESFNILGSNFWNKYFKPYTYSVVRHIEK"
     gene            7652..8002
                     /locus_tag="VKN64_11480"
     CDS             7652..8002
                     /locus_tag="VKN64_11480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMA60897.1"
                     /translation="MPNSYICDKDIFKHIYKLEKRRTGRRKYNSYFSDIKLEQKNDNN
                     IKKARQYIEKILSNKLRSGDVVCKWDINHFVLILNNVDDYGASRAVERIKNHFFENIQ
                     ENNINLEIKYLPIE"
     regulatory      8075..8163
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      8221..8299
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8312..8785
                     /locus_tag="VKN64_11485"
     CDS             8312..8785
                     /locus_tag="VKN64_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60898.1"
                     /translation="MNKKLVVVILTVLLLLSLNVKASEKIVSKTVKVIAYIPVFQEME
                     VVKPIYIKNINDEVKDESLSEPVIIRQAGKIRVKSNSSWSLELTNVTLPENCEIKIKE
                     SESSVWKDVSRGVTNISGENGDHLLSFDLKLVPNGNDNLSSANKSINFNYTLVQI"
     gene            complement(9063..9803)
                     /locus_tag="VKN64_11490"
     CDS             complement(9063..9803)
                     /locus_tag="VKN64_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HMA60899.1"
                     /translation="MFNIEADLHMHTVNSGHAYSTVDELAKTASFKGLKLIAITEHGP
                     NLPGGPHEYFFGNMSIIPNEMYGVKILKGIEANILNDGKLDLSQDRLKSLDFIAAGLH
                     QDTGHKLKTKEDYTNATIEAIKNPMVHMITHPATKYYPIDMEAVVKAASRNDVILEIN
                     ASSFNPKKSNPRGSKELTVELCKLAKKYGVQLSVNSDAHFHTQVGEVHHLFDILKEAG
                     VKDSDLINTSIGKIETFLGDNLIRKISV"
     gene            9951..10796
                     /locus_tag="VKN64_11495"
     CDS             9951..10796
                     /locus_tag="VKN64_11495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60900.1"
                     /translation="MAKQKIKLSKEKETLLVPLYCKACEYEKEKPIIKDEKAKEIIER
                     VEYNFTELDVPNKTCVTLNIRANKLDEYVNQYIISHKNPTIIHLGCGLDNRYGRIKDK
                     NINFYDLDYQEVIELKKNFYQENENYHFISSSVTDLDWINEVGEKPNVLIVAEGLFMY
                     IAEEELKKLFNTLQEQWLNAHLIFDAYSKYTVKNIDKHASIRKTGAEIKWGIDDPQEI
                     EDWNDNIKLVEEWYFTDYQQTEKLSPLYKLAFKAAGLFKMAKKAHRILYYEFKENEVE
                     GNDST"
     gene            10783..11781
                     /locus_tag="VKN64_11500"
     CDS             10783..11781
                     /locus_tag="VKN64_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60901.1"
                     /translation="MTVHEYDENMRPDEEFVEGDYCYLVAGNKCRLLDGRRTTGVIEE
                     YFEESGMFKWRITKYEDEGKCWKLPAEDIKRFQFEKDSKKLGPKSISDIRAAVRKYNS
                     KITIEASNEDREKTQNIIAETKGQAKDWLQENSKFLKENDDIDFTRKEGYESLYQDIK
                     NYMKDHNLLEQEDLTTNSFVLNPASGEWVKGLKIMLAELGLVTYKGKKIRTDDIFKGA
                     GNKLLRRKYIINRLAFLQALFELLAIEEVILYRGMSTEKGWIEKDRTFLSCTFNREVA
                     EAFCCMERDSKYKNSYIFKMTYPVDKLFLTFFETADMNNQYQEAEAIVLYSEEIKI"
     gene            11816..>12276
                     /locus_tag="VKN64_11505"
     CDS             11816..>12276
                     /locus_tag="VKN64_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60902.1"
                     /translation="MKKEDKRSLIIYTVITFVFTWIFWITALYMGYKDIPFLRYLNWD
                     FESSSQVTRHLIFRIGVYGPLVGSLIVTYLYSGGQGLNSFIKKIFKVNIKAKWYLYLF
                     LIPLIINFVVVLIGLIIGININSFFKSSIPINYILVFFIYEVLTSGLEEPG"
BASE COUNT         4633 a         1464 c         2264 g         3915 t
ORIGIN      
        1 tcagctgccg gtggttatgg tataatagcg gctgccaagg ataaagatat aaaatgtatt
       61 atcggtcaat gtccgggtct tgataataat gctgataata atgatgtttt caaaagagaa
      121 gggatcggat tttttataaa actatttata catgcccaaa gagataaagg acgttctaga
      181 tttaaacttt cttctcatac tattccactt gttggtaagc cgcacactac cgccatgcac
      241 acagtcccag gagcttttca aggttatagt gaactagcta cagataattt caaaaatgaa
      301 gtttgtgcac gtttgattct gatgcgtcat cttaatccaa ttgattatgc tgacaagatg
      361 gactgccctg ccctatttca aatttgcgaa aatgaccagt ttgtttcagc tcaatcctat
      421 gtaaaaactg aagaaatatt aggtaaaaaa gccaagttca taaagtatcc gattggacat
      481 tttgatattt ataagggtga aaattttgaa aaagcagtta ataaacagat tgaattttta
      541 aaagagaact ttaattaatt tgtattttta tttaactgag atgaaaagag gtgagaataa
      601 tggttgattt tattataaga gaaattataa atcaaccaag agatatagtt atcaaagcct
      661 ttttgaatcc ggccaatatg atattttgga ctaaagattt ggaaaaattt gaagttatcg
      721 ccgggaaacc gggcgaaata ggttctatag cttatttgca ttatcggcaa aataataaga
      781 ggtataaaat gagagatgaa ctaattgaat gtaatcctgg ttttcgatat ttatctagag
      841 ttacaggaga tacaataact gctactgttg aaacttattt aaaagatgtt ggtaatgata
      901 ctgagctaac aatacattgg acaggcagtg gtaatagttt tttattgaaa atcatattgc
      961 cattgtctaa gagaaaaatg attcatcagg ctaaaaataa attaaaaact tttaaagatt
     1021 tagtagagaa aagaggagtt cattttaata tattcgtaca taaaataagc aaatacacat
     1081 tatagaaaaa ttatcactca aatttaaata attcttccac agttacattt aaaactgcag
     1141 ctatatccat ggcgagtttc aaagatggat tatacttccc ctgttctaga tgaacaatag
     1201 tttccctcct tacatcgacc aaatcagcca attctttttg agttaaatct atctttgctc
     1261 tgtgttcttt tattttggtt tttaattctg gcatgattag aatcaacccc tatcatagat
     1321 gtgaaatgat attgtaaaga ctaatatatg ttgaacaaaa ttaattatca aaccataaat
     1381 aaaaagatcg ggattagaag caagactgcc tatcacaatt gttaaaatga agattatcaa
     1441 tgtagtaata aaagtgtttt ttgctgcttt attaatattt tcttctaacc tttcatctga
     1501 ttttatatta gcaaaaccaa agaatccaaa aaatccaaag aaaccatata aactgggatt
     1561 ggatgtaaat aatcccaaca agcccactat tcccatcaaa cctagatact taatccaatt
     1621 tgttttcatt attatcctcc tcaagttata aatatataac tttatgttat aaatatataa
     1681 cattatcaat taaatgtcaa taaaaatgaa gtgaattaat aaatttttaa tatttaattt
     1741 ttaaatatta ttataaagga gtatttttat tatgaaaaat tggaaaacct atatactatc
     1801 aatattttgg gtccctttat tattaatgca gattgtttta gttatcttta tggggaaagt
     1861 aaatgaagca ggcctaattt ggttatttta tttaggttgg ttaatatgga taatatcaat
     1921 tatcttaggc tggctaccta tatacacact taaaaaaaga ggggcagttc cagctggtaa
     1981 aagttatgtc cataccaaaa agttagtgac ttctgggatt tattctattg ttcgacatcc
     2041 tcagtatact gctgggatgc tcttgagtct tgcattatta ttaatatcac aggatctttg
     2101 ggtcataata attggaatta aagtaatagt tttattatac atagatattt atctaacaga
     2161 taagtgggaa attaataaat tcggtcaaga atataaaaag tatatgaaag aagttccgcg
     2221 agtcaatttt atcttaggtt taattagatt atataagcaa agaaggtgag aaagatgagg
     2281 attaatataa taccaagatc taaatatggt aaatgggcta tttatgcaat actggctttt
     2341 ttcttctttt gggccctgtt ttatcttttg gttgagatgg gtgagagagg tggagccact
     2401 ttttttagta actttaaact aacaatacct tttctagcag cggcaatttc aggaattgcg
     2461 gcactagtta tgggcttatt aagtatattt aaaagtaagg accactgtat tttagtatat
     2521 atttcaataa taattggtct ttttgtatta atctttatta tgggagagat aatttatcca
     2581 cattaggtta tgaataataa gaagaataag agttaaaggg ctggctcatg agattaatat
     2641 gagataaaaa tttgtataat taaattagca ataattatac ttatataatc gaggaattta
     2701 ttcagatata aaaaaatgta ggggttgatt aaagtgccaa gggtagttca ttttgatatt
     2761 aaaactgaag aaccagaaaa attaaaaaaa ttttatgaaa aagtctttaa ttggaagttt
     2821 gataaatgga atgatccaag tggacagatg gaatattatt tagtaaatac cggggaagag
     2881 gagccaggaa tcgatggcgg tcttgctaaa agagaaggtc ccgatgatca gatagatatc
     2941 acgattggag taaaagatat tgagcgttat gcaaaattga tagaagaaaa tggcggtaaa
     3001 atattaagcc ctaaaatgtc aatttcaggg gttggttatt taatgaactt tgaagatccc
     3061 caggagaata agtattcaat aatggaagat gatccagagg taaaataata aattgggtgg
     3121 gtatgagatg aatggttttg agattattga aacaaatgaa gaaaatgtaa tgaaattggg
     3181 tctttgtggt tataaaaata taaagagaga gggctttcct gaaaagattg agtggttgaa
     3241 aaaacaaaaa gatctgggtt tgacaataaa aagtttatat tcaaaagaaa ataaaactca
     3301 agggatgatt gaatatatac cagggcagta ttgctggcgg ccagtaaaag cgaaagacta
     3361 tatgtttatt cactgtctat ttgttggttt taagaaggaa tataaaaata agggttatgg
     3421 ttctctaatg attaaagaat gtattaaaga cagtaaaaaa caaaatttta aagggattgc
     3481 agttgtaact agaaaaggat cttttatgtc aggcaaagag atatttatta aaaataattt
     3541 taaagtagtt gataatgctc ctcctgattt tgaattactt gtttataagt ttaaagaaga
     3601 tactttagat cctaaattta ataataattg gtcagaaaaa caaaaagagt atgggaatgg
     3661 attagtaatt atcagggcga atcaatgtcc atatactgtt aaaaatgtaa atgaaattgt
     3721 taaaacggct aaacaagaat ataaaattaa tgcaaaagtt attgatttaa agaactatca
     3781 agaagctcaa aatagcccct gcccctttgg aactttttgt atcttattta aaggtaaggc
     3841 tattgcaaat catcctatta gtaaaagaag atttatgaat attatgtcta aagaagtggt
     3901 ttaaatgatt acagaattac ataataaaga tattaacatt aataaattag tagcgcaagt
     3961 taacgaagat gaaagtctta ttttagaatt attagataat cttaattcaa aagaggagac
     4021 tattcgttat aatagttatc aaataataaa aggaataagt ttaaaatacc ctgaaaaact
     4081 ttattcttat agggatgaat ttggaaaaaa gttatatatt gacaatgctt atcagatgtt
     4141 aataggaata attatattgg ctcacctttg tgtagtagat aaagatcata agtttgagca
     4201 aatttttaat aaatactgta gtctattaaa cgatagaagg ataactgttt ctttatatgt
     4261 tttaagtaat cttggccgta ttgccaaagt gaaacctaat ttacaagaaa aaattactat
     4321 tatattattg gacagcaaaa atacccacca taaacacaaa ggattaatag caggtgctgc
     4381 tatcgaatct tttgaaaaat attatattga aattgaagat aaagaaaagg tattagagtt
     4441 tgtcaaagag cagattgttt gtgatagtcc taaaacaaga aagtctgcag tagaattttt
     4501 aacaaaatgg gataataaat agtcgatttg aataggatat tgaaaggagg ctttttataa
     4561 atggaagaaa ggcatcctga aatgatattt tgtcaaagat gtgcaatgcc attagttaat
     4621 taagatgatt atggaactaa taaagatgga agtaaaaata tagaatattg taagtaatgt
     4681 tatcaaaatg gtcactttac tgaacctcaa attaaaatgg gaaaaatgat tgaaaagact
     4741 ataggaataa tgaaggaaat gcagatttca gaagagcaaa ttgaaaaaac caagcatttc
     4801 atcccggggc tgaaaagatg gaggcgatag aaatggatag aatagaaaaa attctaattt
     4861 ctaagatcaa gcctaaggaa aaaagccaaa aaattgtaga tatggttatt gaagaaaaaa
     4921 taagaactga agatttcata agttatttta ttatgacttc ggatgtgaaa aaaggcacct
     4981 gtgcagatgc catgaagcat attgctaaat tacgccctga aatcctttat ccttatattg
     5041 atcttttaat tgaatacatt aattataatg caccacgtgt aaaatggggt attcaagagg
     5101 caattggtaa tctggctgag agatatccag atgaagtagc taaggctata ccagggcttt
     5161 taaaaaatac tgaagaaaca aataaaaaca ctactgtagt tagatggtgt gcagcttatg
     5221 gattggcaga aattcttatt aataacaaac aaattcaaaa agatctctta ccaaagataa
     5281 aagaaattgt aagtacagaa aaaaataatg gggtaaaaaa tgtttatcta aaggctttaa
     5341 gaagattaga aagaattaat taaaactcat tgtggaaatc tttattctta tttaacagtt
     5401 ttttaatatt atcaaggagg gatatatttg aggaaaaata ttttttggat atttggtatt
     5461 ttgcagtcag tttctttatc tgtattaatc tatatagtat taaattcttt ggatttgatt
     5521 tataaaggac cgctagtggg aaaggacagt atttttgttt tgagtgcagc ctttcctact
     5581 tttttattga ttgtagaata tattatctat acgaaggaat agtataattt atagcgaatt
     5641 caacagatat agaagaacta attatagagg atgtcaatta aaatggctaa aacagtttat
     5701 tatttcagcg gaaccggaaa ttcattggct gtagctaaag atacagccaa tttaactatg
     5761 gctaaattac tttctattcc ttccctaata gataaagagg aaatagaggt tgattctgaa
     5821 attataggta tagtatttcc cgtataccat caaaacttgc ctaatatagt tagaaagttc
     5881 attctaaaat taacaaatct caaggataag tatatctttg caatctgtac ctatggagat
     5941 agtcccgcta taactttaaa ttatttacaa gagcttctca aagagaaaga ggggaaattg
     6001 ggggcaggat tttcaattaa gatgccctat aattatatca atccctcttt aaatatattc
     6061 aattttttta atacctttac tttaagaaat cagaacgagt aaaccatgaa caaaatgttc
     6121 gatcaatgga aagatagatc aaaatatata gctgatatta ttaacaataa aaaagaaact
     6181 gagatagaaa cagaggctgt tttaattgaa aagttagttg atctttttaa cttacggaat
     6241 actttacaaa agtgtatttg gcttaaagta gtcgggtata atgagtataa atgtttaacc
     6301 tttaatgaag ctattaaatt tttggattat ggctttgaag tagatgatag ttgtaacgtt
     6361 tgtgaaatat gtgcaaagat atgtccggtt aataacatcg caataaaaaa ggggaaacca
     6421 gactggcagc acaactgtga acagtgcttt gcctgcctgc agtggtgtcc acaacatgca
     6481 attcagttta aagaggggac caaaaataag gaaaggtatc atcaccctga gattactcta
     6541 tctgaaatta ttgataataa atatagcaaa ggagcagatt aatggaaata aatattaaag
     6601 aagtaaagga agaatataaa gaatcagccc ttaatcttgt tttggattca tatcaaaact
     6661 taaaaaaaga aatatctttt ttacctacaa aatcaactta caaacagaag ttcaaagata
     6721 aaatagagca tctatttgaa aaaggcctag gagttatagc cttacaagaa gataaattaa
     6781 taggtttttt aataggatat gaggtagatg aattatttgg aaaggataaa ggagtatatg
     6841 ttcctttata cgctcatgct gctgtgaata ataaaaagag agtttatcaa aagatgtata
     6901 ccagggcagc tgaactctgg ataaatagaa gttgtgtcat ccacgcccta actatcttta
     6961 cttgtgataa ggatgtgatt cacgctttct attggttggg atttggatta aggtgtattg
     7021 atgctatcca aaaagttgat ttaattgaaa aaagcagtca taataaggtg aagattataa
     7081 aggcagaatt aaaagagata gaattaatgg aggagttata taaaaaagac aatttatact
     7141 atggaagttc ccctcttttt atgccggtta atcaaaaaag caatatagat aaattaagga
     7201 agtggttaaa caaagacaat catcatctat ggatggcgtt aatagaagat agagtagtgg
     7261 gttatataag aattgaacct acaggagaaa cagtgatatc tacatattct aaaatgatga
     7321 atataacagg tgcatttgta gaggaaaaat atagagataa aagaataggc aaattccttt
     7381 tagatgaagt tttaatatac ttaaaaaaga ataattacat gctttgtggg gttgattttg
     7441 aatcttttaa catactaggg agtaattttt ggaacaagta ttttaagcct tatacttata
     7501 gtgttgtaag acatatagaa aaataattta aaaattaata actaaagaag gtaaagaaat
     7561 agttttgaac aatattaata gtagaaagat aattttttaa aataattatt ataaactcaa
     7621 atatcacttc ttgaaaggag gaagtgactt aatgccaaat tcctacatat gcgacaagga
     7681 tatttttaaa catatttata agttagaaaa acgtaggaca ggacgaagaa aatataattc
     7741 ttatttttct gatataaaat tagaacaaaa aaatgataat aatataaaaa aagctcgtca
     7801 atacatagaa aaaattttga gtaataagtt gagaagtgga gatgtagtat gtaagtggga
     7861 tatcaatcac ttcgttttaa ttctcaataa tgtcgatgat tatggtgcaa gccgagctgt
     7921 agaaagaata aaaaatcatt tttttgaaaa catccaggag aataacatca atttggaaat
     7981 caaatattta ccaatagaat aattgcaaaa agttattgct aaaagtcatc agtcctgata
     8041 taataattac aaataatgaa tatatattca cccaaacaga gaagggctac ttaatctgaa
     8101 aggattaaga cgcaaagttt taacctacga cttttagtaa gttatggttg ttaaactacc
     8161 taaggtggtt gaatcttacc tgtctctgat taaaaattag gacaggtttt tattttgcct
     8221 tcttttcagg ggtaaactta tcgtgagata aggacacaaa gccaagggtc ttataaaatt
     8281 aagacagccg gctgcaagag gaggaaatta tatgaataaa aaattggtag ttgttatctt
     8341 aacagtatta ttactgttga gcttaaatgt aaaagcctca gagaaaattg ttagtaaaac
     8401 tgtaaaggtt atagcttata tacctgtttt tcaagaaatg gaagtagtaa aaccgatcta
     8461 tattaaaaat attaacgatg aagttaaaga tgaaagttta agtgagccgg tgattatcag
     8521 gcaggccgga aagataagag tgaaaagtaa cagttcttgg agtctggaac taacaaatgt
     8581 tactctcccg gaaaattgtg agataaagat taaagaaagt gagagctctg tgtggaagga
     8641 tgtatcaagg ggagtgacaa atatttccgg tgaaaatggt gatcacctgc ttagttttga
     8701 tcttaaatta gttccaaatg gaaatgataa tttaagctca gctaataaaa gtattaattt
     8761 taactatact ttagtccaga tataataaaa aatataataa aaattctaag atcctctgct
     8821 tattttactt gaaatatggc aggggttttt tctatgtatg aacataacag acaaaatatt
     8881 taaactgaaa tatgaaaggt ttgttgaatg aattaaaaag ggaaagaaaa taatgtaaat
     8941 aaattgaagg agagcaaagt taaggcgaga attaaataat atgtaagcaa tttaaaaaga
     9001 taaaagtaat gattataaat atcatatggt agtgatattt acatcatttt atctttttaa
     9061 atttagacac tgatttttct tattaaatta tcacctaaga atgtttctat ttttccaatg
     9121 gatgtattaa ttagatcgct atctttaaca ccggcttctt ttaagatatc aaataggtga
     9181 tgtacttctc ctacctgagt gtggaaatgg gcatcactat tgacacttaa ctgtacaccg
     9241 tatttcttgg ccagtttaca tagttcaact gttaattctt tcgaacctct tgggtttgac
     9301 tttttcggat taaatgaact ggcgttaatt tctaagataa catcattccg gctggccgct
     9361 tttactacgg cttccatatc gattggataa tatttagtag caggatgtgt tatcatatgg
     9421 accatcggat ttttgatagc ttctattgta gcatttgtat agtcttcttt ggtctttagc
     9481 ttatggcctg tatcctgatg taagccagct gcaataaagt cgagtgactt taatctgtct
     9541 tgactgagat cgagcttacc atcattcagg atattagctt caataccttt taagattttt
     9601 acaccataca tttcattagg tatgatactc atgttaccaa agaagtattc atgtggacct
     9661 cctggtaaat tagggccgtg ttcagtaatt gctattaatt ttagtccttt aaatgaagct
     9721 gtcttggcta attcatcgac tgttgaatag gcgtgaccac tatttacagt atgcatgtgt
     9781 aagtctgctt cgatattaaa catatagttt acctcctttc tgatttttaa aatgttcatc
     9841 ttgcttatat tataataaat aactattaca gtcaaattaa gagtgtatta atagaatgtt
     9901 aaaaataatt ataaataaga aaaaagttga taaattatgg gggaattata atggctaagc
     9961 agaaaattaa attaagtaag gaaaaagaga cactcctggt gcctttatac tgtaaggcct
    10021 gcgaatatga aaaagagaaa ccaattataa aagatgaaaa ggccaaggag attatcgaaa
    10081 gagtagaata taattttacc gagttggatg tacccaataa gacctgtgta acattgaata
    10141 tcagggcaaa taaattagat gaatatgtga atcaatatat tattagtcat aaaaatccta
    10201 ccataattca tctaggctgt gggctagata atagatatgg aaggattaaa gataaaaata
    10261 ttaattttta tgatcttgac tatcaagaag tgatagagct taaaaagaac ttctatcaag
    10321 aaaatgaaaa ctaccatttc atttcttctt ctgttacaga ccttgattgg ataaacgaag
    10381 tgggtgaaaa gccgaatgtt ttgatagtcg cagaaggttt gttcatgtat atagcagagg
    10441 aagaattaaa gaagttattt aatactttac aagaacagtg gctaaacgcc catttgatat
    10501 ttgatgccta tagtaaatat actgttaaga atattgataa acacgcttca attagaaaaa
    10561 caggcgcaga aatcaaatgg ggtattgatg atccgcaaga gatcgaagat tggaatgata
    10621 atattaaact ggttgaagaa tggtacttta ctgattatca acagacagag aagttgtccc
    10681 cactttataa attagcattt aaggcagcgg gactatttaa gatggctaaa aaagctcaca
    10741 ggatactata ttatgagttt aaggaaaacg aggtggaagg taatgacagt acatgaatat
    10801 gatgaaaata tgaggccaga tgaagaattt gttgaaggtg attactgtta tcttgtagcc
    10861 ggtaataagt gtcgattatt agacggaaga agaactaccg gagttataga ggaatatttt
    10921 gaagagagcg gtatgtttaa atggagaatt accaagtatg aggatgaagg gaagtgttgg
    10981 aagttaccag cggaagatat caaaagattt caatttgaaa aagactctaa aaaactcggt
    11041 ccaaaaagta ttagtgatat tagagcagcc gtcagaaagt ataattcaaa gataactata
    11101 gaagctagta atgaagatag ggagaaaacg caaaatataa tagcagaaac caaaggacag
    11161 gccaaagatt ggttgcagga aaattctaaa tttttaaaag aaaatgatga tatagatttt
    11221 accagaaaag aaggttatga gagcttatat caggatatta aaaattacat gaaagaccat
    11281 aacctgcttg agcaggagga cttaacaact aacagctttg ttttaaatcc ggcctcagga
    11341 gaatgggtta agggtttaaa aataatgctg gccgaattgg gtttggtgac ctataaggga
    11401 aaaaagatca ggactgatga tatctttaaa ggagccggta ataagttgtt gcgcagaaaa
    11461 tatataatca acaggttagc ttttttacag gccctatttg agttgcttgc aatagaagaa
    11521 gtaatattat atagagggat gagtaccgaa aagggctgga ttgaaaaaga tcggaccttc
    11581 ttatcctgta cttttaaccg agaggtagca gaggctttct gctgtatgga aagagacagc
    11641 aagtataaga actcctatat atttaaaatg acctatcccg tcgataagtt attcctgacc
    11701 ttttttgaaa cagccgatat gaataaccag tatcaggaag cagaagcaat tgttctttat
    11761 agtgaagaaa taaagattta atattattgg ataagggctg aagaaaggag taaaaatgaa
    11821 aaaggaagat aaaaggtcct taataattta tactgtgata acctttgttt ttacctggat
    11881 tttttggatt acggccttat atatgggcta taaagacatt ccctttttaa ggtaccttaa
    11941 ttgggatttt gaaagttcca gtcaggttac caggcatctt atctttagaa ttggagtata
    12001 tgggccatta gttggttcct taatagtgac ttatctttat tcaggtgggc aaggattgaa
    12061 tagtttcata aaaaagatat tcaaagtaaa tataaaggca aaatggtatt tatatttatt
    12121 tttaattccg ctcataataa actttgtagt agtattaata ggtttaatta ttggtattaa
    12181 tatcaattct ttttttaaaa gcagtattcc tataaattat atattagtgt tttttattta
    12241 tgaagtatta acaagtggac tggaagaacc gggttg
//
LOCUS       DATGCJ010000261         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_88044, whole genome shotgun sequence.
ACCESSION   DATGCJ010000261 DATGCJ010000000
VERSION     DATGCJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88044"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VKN64_11510"
     CDS             complement(<1..278)
                     /locus_tag="VKN64_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60903.1"
                     /translation="MRNSKSILFVSNGHGEDLIAANLIKSLLAQDNNLSISELPVVGK
                     GNTFEKMPVEILGPRETLPSGGFARNGWQNLWNDLTGGLISNTYKQIQV"
     gene            complement(300..2018)
                     /locus_tag="VKN64_11515"
     CDS             complement(300..2018)
                     /locus_tag="VKN64_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60904.1"
                     /translation="MEIYKRFGKYMRPYMGEIIGGLVCLIIVGILGILVPWLFKFLID
                     DVLINGNEVILNYIAGGAILLYLLKGIFNYGQKYLISGMSQHVVVDIRKDLYQHLQKL
                     SLSFFERQRTGDLMSRITNDVNVIQNSLTHGLSNFILQPIMIIGIIGFLLYIDWKLAL
                     VSFIIIPLVSYAINKFGFKMRSISSRIQNEMSNVTNILQETLSAVRIVKAFNNEDKEI
                     KKFSEANERTLQENLKGVQVEATITPIVEIIIALGSAAFLWYGGHKVLRGAMTTGELI
                     TFIGYIGLLVSPINILSRSYNRIQKGVGAAERIFKLLNIEEKVVEKDNAKKMPAIKGE
                     VVFDEVDFSYKNDEKVLKDINLKIKPGEMIAIVGHSGAGKSTLVNLIPRFYDVDSGEV
                     RIDGINIKDVTLKSLRKQIGIVPQETFLFKGTIEENISYGVENKTRDEVIEAAKKANA
                     HQFIQEFAQGYDTEVGERGVSLSGGQKQRISIARALLTEHKILILDEATSSLDLKSES
                     LIQEALEYLIKDRTTFVIAHRLSTIINADKIVVIEEGKIVEKGPHQELLDKEGYYYNL
                     YKKQFK"
     gene            2278..3186
                     /locus_tag="VKN64_11520"
     CDS             2278..3186
                     /locus_tag="VKN64_11520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMA60905.1"
                     /translation="MVSEIKASVVIPTKDRKEILKKCLTALNKQTYDFNKFEIVIIDD
                     GSSQNNKRMIESLNLNLSINYYFQENSGPAKARNHGIKKAKGEYIIFIDDDIIVNNKF
                     IEAHLNQHEDNNKVIVHGPVIYTNDLDNPTSAEKKLSDFSNAFFATGNASIKKEYLFE
                     AGLFNENFYEYGWEDLEFGKRLKKLDLEAVQAKEAKGYHLKHKFTIKDIPKLRSKERQ
                     RGRMAVLYHDLNPSFSVKSSTLYWKPFLILMEILTIFNWPRSKFTKKLVEFFHNKSCK
                     YTRNFILYFVKLDAYLIGLKEGYEKR"
     gene            3288..3950
                     /locus_tag="VKN64_11525"
     CDS             3288..3950
                     /locus_tag="VKN64_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60906.1"
                     /translation="MKNFSKILIILSLLILISTPIQAESDWTFEFFGGTGLNIPLPLR
                     IEEAGGTDIIIESAEYSTRPLEGAPYYAYRFGKWDNNKAWEIQLVHHKIYLENENPRI
                     DNFSISHGYNLITANRAWKTDDFTYRIGAGVVLTHPEFTIDGVSNDQKLGLGGAGFYL
                     DGITGQVAISKSFEITKKIFATTEAMYTASYAKVNPDDYDYTATVPNSALHFLIGLGY
                     NF"
     gene            complement(4005..4574)
                     /locus_tag="VKN64_11530"
     CDS             complement(4005..4574)
                     /locus_tag="VKN64_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMA60907.1"
                     /translation="MQMRKIIDQQKLFILIILLSLLFVFSSLTFADDFDQEIRTMVLE
                     SETEFIDEAMAGITHLGNGAVDYAIASALPDKEARIDAQRSLLVGGVSAFVLKTVIGQ
                     KRPPGPIEYKHFTLDSHYHAMPSGHTTTAFALAATIARHYPKYKYLSYGLATMVGVSR
                     LYEDVHWASNVVAGAGLGYISAKFVEIKW"
BASE COUNT         1589 a          870 c          660 g         1588 t
ORIGIN      
        1 acttgaatct gtttatatgt attacttatt aaaccacctg tcagatcatt ccataagttt
       61 tgccagccgt tgcgggcaaa acctccgctt ggtaatgttt cgcgagggcc cagtatctca
      121 acaggcattt tttcaaaagt atttccttta ccaactacag gtagttctga tatggaaaga
      181 ttattatctt gagcaagcag agattttatt aaattagcag cgatcaaatc ttctccatgt
      241 ccattactaa caaataatat actttttgaa tttctcatat tatcaatccc aattaaaaat
      301 tatttaaatt gttttttata taaattataa taatagcctt ctttatccag tagttcttga
      361 tgtggtccct tttcgacaat ttttccttct tcaataacta caatcttatc agcatttatt
      421 atggttgata gacggtgggc aatcacaaaa gtagtcctgt cctttattaa atattctaaa
      481 gcttcctgaa ttagtgattc ggatttcaga tctaaagaag aggttgcctc atccagtatt
      541 aaaattttat gttcggtcag aagggccctt gcaatggaaa tacgttgttt ttgtcccccc
      601 gatagtgaga cgcctctttc ccctacttct gtatcataac cttgtgcaaa ttcctgtata
      661 aactgatggg cattagcctt tttagcagct tctatcactt catctcttgt cttattctcc
      721 actccatatg aaatgttttc ttcaatagta cctttaaata aaaaggtctc ttgcggcacg
      781 attccaattt gttttcttaa ggatttaagt gtcacatctt taatattaat tccatcaatt
      841 cttacctctc ctgaatccac atcataaaaa cggggtatta agttaaccag agtagatttc
      901 ccggctccac tgtgaccaac tatagcaatc atttcacccg gctttatctt taagttaata
      961 tcttttaaga ccttttcatc attcttataa ctgaaatcta cctcatcgaa tactacttcg
     1021 cctttgatag caggcatttt tttggcatta tctttttcta ccactttttc ttctatattc
     1081 aataatttaa atattctttc tgcagcaccc acaccttttt gaatcctatt ataacttcga
     1141 cttaaaatat tgattggact gaccagaagt ccgatataac caataaatgt aatcagttcc
     1201 cccgtagtca tagctcctct taatacttta tgccctccat accataaaaa tgcggctgag
     1261 cctaaagcga taatgatctc aactatcggg gtaattgttg cttcaacctg gactcccttt
     1321 aaattttcct gtagggtcct ttcgtttgct tcagaaaatt tcttaatctc tttgtcttca
     1381 ttattaaacg ctttcacgat tcttaccgct gataaagttt cttgtaagat atttgtcaca
     1441 ttactcattt cattttgaat tcttgagctg atcgacctca tcttaaatcc aaatttgttt
     1501 atggcataag atacaagagg gataataata aaagatacta atgctaactt ccagtctatg
     1561 tataataaaa accctataat acctattatc atgatcggtt gtaaaataaa gttcgataaa
     1621 ccatgggtta aactgttttg tattacatta acatcattag taatccggga cattaaatct
     1681 cctgttctct gcctctcgaa aaagcttaga gataattttt gtaaatgctg gtataaatct
     1741 tttctaatat ccactacaac atgctggctc atacccgaaa ttaaatattt ttgaccataa
     1801 ttaaaaatac ctttaagtaa gtataataaa atagctcccc cagcaatata attaagtata
     1861 acttcatttc catttattag cacatcatca attaagaatt taaataacca gggtacaaga
     1921 atacctaaaa tacccactat aattaagcat actaatcccc ctataatttc ccccatatag
     1981 ggacgcatat attttccaaa tcttttatat atttccaatg attatacctc cataaatatc
     2041 actattaata ttatatattt aagtttctat ttcactttat attttaaact cccaacgaaa
     2101 gcaacaattc caatatattt ttagcaattt aattttaaca cagacaagaa agttttgaaa
     2161 gatattttac tagaataaag caatattgct ctgataaagc ataaatactt gaacacttac
     2221 cactcataat atataataaa acaagattat atataaataa atggagtgtt ttaactcatg
     2281 gtttccgaaa ttaaagcaag tgttgtaatt cctaccaaag atagaaaaga aatattaaaa
     2341 aaatgcctaa ccgcactcaa taaacaaaca tatgatttta ataaatttga aatagtaatt
     2401 attgatgatg gatcatcaca aaataataaa aggatgatcg aatcattaaa tttaaacctt
     2461 tccataaatt attactttca agaaaatagt ggtcctgcta aggccagaaa tcacggtatt
     2521 aaaaaggcta aaggtgaata tattattttc atcgatgacg atatcattgt aaataataaa
     2581 tttattgaag cccatttaaa tcaacatgag gataacaata aagttatcgt tcatggtcca
     2641 gtgatatata ccaatgattt agataaccct acctcggccg aaaaaaagct cagtgacttt
     2701 tctaacgcct tttttgctac cggtaacgcc tcaataaaaa aagaatatct ttttgaagca
     2761 ggtctattta atgaaaattt ttatgaatat ggctgggaag atttagagtt tggaaaaaga
     2821 ctcaaaaaac tcgacctaga agctgtgcaa gctaaagaag caaaaggtta tcacctaaaa
     2881 cataaattta ctataaaaga tatccctaaa ttaagatcca aggagaggca gagaggaaga
     2941 atggctgttt tataccatga tctaaatcct tctttttcag taaaaagttc aactctttac
     3001 tggaaaccct ttctaatcct tatggaaatt cttactatct tcaattggcc caggagtaaa
     3061 ttcaccaaaa aactagtaga atttttccat aataaatcat gtaaatatac aagaaatttt
     3121 attctttatt ttgttaagct ggatgcctat ctaatcggat taaaagaagg ttatgaaaaa
     3181 agatagttat taagagttta acaaatgata aaaatatcaa aaatattggt cttcaattaa
     3241 atttagaagg ctgaaataaa aaatacagta taggaggaat acataatatg aaaaatttta
     3301 gcaaaatcct tattatttta tctttattaa ttttaatctc tactccaatt caagctgaat
     3361 ctgattggac ttttgaattt ttcgggggta cagggttaaa catacctcta ccattgagaa
     3421 tagaagaggc gggtggaacc gatataatta tagagtcggc cgaatactct actagacctc
     3481 tagagggagc cccctactat gcttatagat tcgggaaatg ggataataat aaggcatggg
     3541 aaatccaatt agtccaccac aaaatttatt tagaaaatga aaacccaaga attgataact
     3601 tttctatctc acatggatat aatctgatta ctgcaaatag agcttggaaa actgacgatt
     3661 tcacatacag aattggggcc ggtgtagttc tcacccatcc cgaatttact attgatggag
     3721 ttagtaatga ccagaaattg ggactgggtg gagcaggctt ttatctggat ggtataacag
     3781 gtcaagtagc tatttcaaaa tcatttgaga tcaccaaaaa aatatttgca acaaccgaag
     3841 caatgtatac cgctagttat gctaaagtaa atcctgatga ctatgactat acagctactg
     3901 ttcctaattc agcacttcac ttcctaattg gtttaggata taatttttaa ttaaataatc
     3961 acttaaaagc ctcagttttc atactgaggc ttttctatat ttaattacca ttttatttct
     4021 acaaatttag cactaatata accgagcccg gcacctgcca caacattcga agcccaatgc
     4081 acatcttcat ataatctact tactcctacc atggtggcta atccatatga tagatacttg
     4141 tattttggat aatgtcttgc aatggtggca gcaagggcaa aagctgtagt agtatgacca
     4201 gacggcattg catggtaatg actatccagg gtgaaatgtt tatattcaat cggaccaggg
     4261 ggtctttttt gtccgataac cgtttttaat acaaaagcac tcactccccc caccagtaga
     4321 gagcgttgag catctattct cgcctcttta tcaggtaagg cagaagcaat tgcataatct
     4381 actgccccat ttcccagatg agtaatacct gccatcgctt catctataaa ttccgtttct
     4441 gattctaaaa ccatagttct gatttcttgg tcaaaatcgt ctgcaaatgt taaactagaa
     4501 aaaacaaata ataatgacaa taatatgatt aaaataaata atttctgctg atcgataatt
     4561 tttctcatct gcatttaagc ttctcctcca cattaattaa attatatttt ttacctaata
     4621 tttatattgt ttaaatatta acattcccta aatcttttaa aatgtcttcc actattttat
     4681 ttacagcatt tttctgtccc tcaaaac
//
LOCUS       DATGCJ010000262        10355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_88164, whole genome shotgun sequence.
ACCESSION   DATGCJ010000262 DATGCJ010000000
VERSION     DATGCJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88164"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="ribD"
                     /locus_tag="VKN64_11535"
     CDS             complement(<1..621)
                     /gene="ribD"
                     /locus_tag="VKN64_11535"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMA60908.1"
                     /translation="MVKKDDTYYMNKALELAKKGEGRVSPNPLVGAVVVNDNEIVGKG
                     YHKKAGTPHAEVHAIEDAGDQAEGATLYVNLEPCCHIGKTPACSIKIINSNIKRLVVA
                     MEDPNPLVAGKGFKQLKEAGIEVKLGVLEERAKKLNEIFIKNMREKSPFIYLKTAQTL
                     DGFLATRTGDSRWITNEKARAYGHRLRHKVDGILVGIGTVLSDNPRL"
     regulatory      complement(709..824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(906..2096)
                     /gene="ribA"
                     /locus_tag="VKN64_11540"
     CDS             complement(906..2096)
                     /gene="ribA"
                     /locus_tag="VKN64_11540"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HMA60909.1"
                     /translation="MNQIDEALADLKAGKMVILVDDGQKENDGDLLLAAEKADPAKIN
                     FLIKEVRGLITVPLSKAKIKELNLPRSGNYDSDKKDDTITVAVDHIKTKEGISAKDRA
                     LTINKLAAGDNPEKFQRPGHVFPLSAKEGGVLRKAGKVEAALDLMKLAGLKEAAVLTK
                     FIDDEGEVAQLPEINEFAQTHNLKVISIKDLIKYRKKTDKLIKEAANAKLPTKFGDFK
                     IKIFKSEFDNKEHIGIIKGDIKNKEEVLVRVHSQCITGDIFGSLRCDCGEQLAAALKM
                     IEEEGEGLVLYMRQEGRGIGLTNKIKAYSLQDEGYDTVEANQELGFEADLRDYGIGAQ
                     ILSELGLKTIKLITNNPKKVVGLEGYGLEITERVPLEIEPHEENEFYLKTKKNKLGHM
                     LDLD"
     gene            complement(2111..2764)
                     /locus_tag="VKN64_11545"
     CDS             complement(2111..2764)
                     /locus_tag="VKN64_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60910.1"
                     /translation="MFVGVIQEVGQVKEKTIENNKINIYIQVNMLSKELEIGNEVNIN
                     GLSFVVNEVGENYFKGTGNLSIMVNENVENLEAGSPVNLEKEVQPYRYKGNHVISGNI
                     NNIGEVIEISQNKDSKKLRVKTIKDLHDYFKENKAIAINGVSLTINKLDEDEIVIKVN
                     EIIWQNTNLKRLSKGDKINIETDIVGRYIGQIMDKQEEIIEKKEMIKENLLNEDEQL"
     regulatory      complement(2894..3019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3125..3877)
                     /locus_tag="VKN64_11550"
     CDS             complement(3125..3877)
                     /locus_tag="VKN64_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMA60911.1"
                     /translation="MAAKIMLEKPIPVTENFDWKKVKEIKIKSSNEKLVSLNMIPERI
                     IVRPQYFIQGIEGSLPECYVREGVYDNLLKAADQLPAGYKFLIFDAWRPESVQYELFD
                     RLKKEIKRENPDIGEDILKEKVEEYVALPSHKMDSPSPHITGGAVDLTIIDNKGKELG
                     MGTGFDQTTGETHTDYYERLKNKRQLNQKEKNILKNRRLLYNTMISADFTNYPHEWWH
                     YDFGNQVWAFMGEKEHAFYSVAEPELRWKHSY"
     gene            complement(3914..5197)
                     /locus_tag="VKN64_11555"
     CDS             complement(3914..5197)
                     /locus_tag="VKN64_11555"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HMA60912.1"
                     /translation="MSILMVISFLILLLAGVPIFIALLISSFFGFLYLGDFSLFRIMV
                     QQFFGGMDIFTLLAIPLFIMTGNLMNRTGITDKLLDFSRVLVGHLKGGLGHVNVVASI
                     IFAGISGSAAADTSAIGSILIPAMAKEGYDRPYAAGITAGSSLIGPIIPPSIFMVLYG
                     SMTNQSIGGLFVAGIIPGLLLGGAFMIMNYYYSKKYNYPVSNERASIKKILKTAWESL
                     IALIAPIIIIGGIITGVVTPTEAGAVAVFYTAIVGFFVTKKLSLKDFAASVNETVRNT
                     SVVFVIMGSAAVFSWLLKWEQVPQKFAGYMLTLTDNNTVLLIILSLMIFVIGMFMEEI
                     AALILLTPVVAPVAMYAGINPLHFGIVMTLNITIALITPPMGACVYIVSAVGKVKLET
                     LFRRIWPFVAVAMTVLLLIILFPQLVTTLPHLFNL"
     gene            complement(5194..5709)
                     /locus_tag="VKN64_11560"
     CDS             complement(5194..5709)
                     /locus_tag="VKN64_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HMA60913.1"
                     /translation="MNNNSISNGLLKKISNYIKYLSMVLAGVFLVINISDILISIFFR
                     YFLNDSLVWTEEVARFTLIYSVMFGSVYALSFGDHVKITIVLEYLPDIISKIINFIVH
                     LLIIGIIGYMTYLGIHYVDNAWRFQTLALGIPKAIPLMSIPIGMGLFLVQYILVEILG
                     INTEDKMEMRE"
     gene            complement(5712..6743)
                     /gene="dctP"
                     /locus_tag="VKN64_11565"
     CDS             complement(5712..6743)
                     /gene="dctP"
                     /locus_tag="VKN64_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HMA60914.1"
                     /translation="MSKKLILLLVLSLLIFSAVGVVGAQESINVKMSYNGPADVETNA
                     VHEYAVNFKEFVEMGTNGRITITLYSDSQLGNEEQRMEQTMSGSTKINVASNAGMVSV
                     YPEIYAATIPFMFESFEAAHKFFDSSEYWEMTQAEFEEKTGVALLEAVEEGGFLAFTN
                     NQKEIQSPSDFEGLKFRGMDPGQIALYESFGASGTPIPWTELYMALQTGVVDGQMNPP
                     TYIISGSLYEVQDYVTMANIQYSCQWLTINADWLNSLPKEDQYVIRQAAKAANIRNRI
                     SVEAKVSERVDFLEENGMQVYYPTEEEMKQFQEIGQPDYISWIEDKIDASYVKEALES
                     AEKANMEAK"
     gene            6928..7470
                     /locus_tag="VKN64_11570"
     CDS             6928..7470
                     /locus_tag="VKN64_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMA60915.1"
                     /translation="MKIGKKIKKYRKLRNITISELAEEADLSVGMISQIERDKIGLSV
                     SSLWKIAQALNVSIGDFFEEETPGDKYVVRKDNRKKIKVTDSTATYELLTPDLSWNIE
                     FLKITIMPGESTDNKKISHKGEETGYIIKGKLLIKWGEKEFILNKGDSIRLDSTIPHR
                     YINIGDEKSVSIWAMTPPSF"
     gene            complement(7473..8138)
                     /locus_tag="VKN64_11575"
     CDS             complement(7473..8138)
                     /locus_tag="VKN64_11575"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMA60916.1"
                     /translation="MSKYDDEVIEIIKDGPYIAYNVKLMLNSKGEEMETSSRMSLCRC
                     GHSDDHPFCDGSHMDVDFDDEKKEDRVSDKVIKYEGENITIYDNRGICSHIGYCTNLL
                     PKVFDKTRFKWIDPDAADAKDIIRICELCPSGALSYSLPGGERVKKVGNQRKKIRISP
                     GPYGKNGPFDIEGGIKLKSDRDHAPECEEHYSLCRCGASKNKPFCDGSHRFYENYKES
                     EEK"
     gene            complement(8264..9007)
                     /locus_tag="VKN64_11580"
     CDS             complement(8264..9007)
                     /locus_tag="VKN64_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMA60917.1"
                     /translation="MGALLYSFIAGVSTALGVVVLLIFGKPKEKFLAMLLGFAGGIML
                     AISVFELLPEAVVLSSMTISIISFIFGALMMFGLDKLIPHSHLSTTDHLEIENPEKIK
                     MNLSMLRTGYLIFFGIGLHNLPEGIAIGAGFESSPEIGLYIAIAIALHNVPEGLAIAG
                     PLKTAGIKNLKIIIFALGAGLMTVVGTFISLLIFNITKVFVGGFLAFAAGAMIYIVND
                     ELIPQSNIIHNHFANAGIIIGLLLGFAML"
     gene            complement(9022..9792)
                     /locus_tag="VKN64_11585"
     CDS             complement(9022..9792)
                     /locus_tag="VKN64_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMA60918.1"
                     /translation="MIKSKRLNTFYGALFIIIFWGILHIIFNSSIVPSPLTVIINFFD
                     ILIPVLLPHLAMSLYRIITAVILALLIGVAIGLLVGINDKVDQYISPVIYLIYPIPKI
                     AFLPLFMLLFGLGDRSKIILIMVIVIFQIIVTTRDSVKNINPEYFYSARSVGVKGIQT
                     YKHVIIPAILPQILTALRITVGTSIAVLFYAENFATQYGIGYYIMNSWVMVNYVNMFS
                     GILAISILGLLIFQGIDLIEEYYCHWVKAGNKKETDII"
     gene            complement(9789..>10355)
                     /locus_tag="VKN64_11590"
     CDS             complement(9789..>10355)
                     /locus_tag="VKN64_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMA60919.1"
                     /translation="KIKIDDKIINSIRKETGVILQNYGLFPWKNVFNNVALGLQVRDY
                     DKDKIENTVFQVLKKLSIYKFRDKFPAELSGGQSQRVAVARSLVLKPDLLLMDEPFSA
                     LDAITREEMQNLILKIHQENNLSYLIVTHNIDESVFLGQKIAVMKEGEFIHQINNPYF
                     GDIKLREKEEFFKLCKKLRTMMSEDDLL"
BASE COUNT         3229 a         2075 c         1484 g         3567 t
ORIGIN      
        1 taagcgggga ttatcgctta aaacagttcc aatgcccact aaaatcccat cgaccttatg
       61 tcttaatcta tgtccataag cccgcgcctt ttcatttgta atccacctag aatctcccgt
      121 tctagtggct aaaaagccat ctaaggtttg cgcggttttt agatatataa aaggggactt
      181 ttcccgcatg tttttaataa atatctcatt taattttttg gctcgttctt ctagaacacc
      241 caatttcact tcaataccgg cttcttttaa ctgtttaaaa cccttaccag ccactaaagg
      301 attggggtct tccattgcca cgaccaatct tttaatattg gaatttatga tttttataga
      361 acaagctgga gttttgccga tatgacagca gggttctagg tttacataca gggtcgcacc
      421 ttcagcttga tctccggcat cctcgatcgc atgtacttca gcatgcgggg taccggcctt
      481 tttgtggtaa cctttaccaa ctatctcatt atcatttact accactgccc ctaccagtgg
      541 gttgggacta actctgcctt cccctttttt agcaagttct aatgctttat tcatatagta
      601 tgtatcatct tttttaacca tctagatacc tccacctatc ctttttttag gacaaaataa
      661 aataacccct ccgtggttga aggggttatc tgaataaaat aaatttatat atactaactc
      721 ccttccagac tatactgtcg gttctggaat tgcaccagat caactattac tagctcgtgg
      781 actataccac cggtatggaa tctcaccaat ccctgttagt tattttcttt gttttctaat
      841 aatattataa gataaaagtt aacactttta aagtaatttc ttttttgaag attttattta
      901 aggccttagt ctagatctaa catgtgccct aatttatttt tcttggtttt tagataaaac
      961 tcgttttcct catgaggttc tatttcaagg ggtacacgct ccgtaatttc aagtccataa
     1021 ccttctaatc ctactacttt ttttggatta ttggttatca atttaattgt tttcaggcct
     1081 aattcagata atatttgggc accgatccca taatctctta aatcggcctc aaagcctaat
     1141 tcttgattgg cttcgacggt atcatatcct tcatcttgca gtgagtaggc tttaatctta
     1201 ttggttaagc ctattcccct gccttcctgt ctcatatata atactaagcc ctcaccctct
     1261 tcttcaatca tctttagggc cgcggctaac tgttcaccac agtcacatct taatgagcca
     1321 aagatatcac cggtaataca ttgggaatga accctaacca ggacctcttc tttgtttttt
     1381 atatcacctt taataattcc aatatgctct ttattatcga attcggattt aaatattttt
     1441 attttaaaat cgccaaattt ggttggcagc tttgcattgg ctgcttcttt tattagttta
     1501 tcagttttct tcctatattt aattaaatcc tttatactta tgacttttaa attatgagtt
     1561 tgggcaaatt cattaatttc aggaagttgg gcaacttctc cttcatcatc aataaactta
     1621 gttaatacag cagcttcttt taagccggct aatttcatta aatctagggc tgcttcaact
     1681 ttaccggctt tccttaaaac tccaccttct tttgcgctta aaggaaaaac atggcccggc
     1741 ctttgaaatt tttctggatt atcaccggct gctaatttat taattgtaag ggctctatct
     1801 ttagctgaaa taccctcttt tgttttaata tgatcgactg caactgtaat agtatcatct
     1861 tttttatcac tatcataatt acccgatcta ggtaaattta gttcttttat ctttgctttt
     1921 gataggggaa ccgtgattaa accacgcact tctttaatta gaaagttaat tttggcggga
     1981 tcggcttttt cagccgctaa taaaaggtcg ccatcatttt ctttttgacc atcatcaact
     2041 aagataacca tcttgcctgc cttgaggtct gccagtgctt catcaatttg gttcatggct
     2101 ctcactccca ttaaagttgt tcatcctcat ttaataaatt ttcctttatc atttcctttt
     2161 tctcaattat ttcctcttgt ttgtccatga tttggccgat atatctacca acaatatcag
     2221 tttcaatatt aatcttatca cctttactta aacgtttaag attggtattt tgccagatta
     2281 tttcatttac cttaattact atttcatctt cgtctaattt attaattgtc aaacttacac
     2341 cgttaattgc tattgctttg ttttctttaa aataatcatg gaggtctttg atcgttttca
     2401 ctctcaattt tttgctatct ttattttgac ttatttcgat aacctcaccg atattattaa
     2461 tattgccgct aattacatgg ttgcctttgt atctataagg ttggacctct ttttctaggt
     2521 tcacggggga ccccgcttct aaattttcta cattttcatt aaccatgata ctcaaattgc
     2581 ctgtaccttt gaaataattc tctcctacct cattaacaac aaatgacaaa ccattaatat
     2641 taacttcatt accaatttct aattctttag aaagcatatt tacctgaatg tatatattta
     2701 ttttattatt ttcgatagtt ttttccttga cttgcccgac ttcctggata actcctacaa
     2761 acataagatc actcctgttc ttttttttat tattattaat atgatattaa tcctcgttta
     2821 aatgtatcga tattattatt tatatcaata acaaacccct tctgatcaga aggggttaat
     2881 tatttatatc tataaacact aactcccttc cagactatac tgtcggtcct ggaattgcac
     2941 cagatcaact attaaaatat aaaatagttt gtggactctg accaccggta tggaatttca
     3001 ccaatccctg ttagcttatt tttttatctt aattaatatt atcatcattg agtaatcttg
     3061 taaagtttat atattacaat ttttctagta aaagtcaatt aatctaattt atttgaaata
     3121 ttttttaata actatgtttc cagcgcaatt caggttcagc tacactataa aatgcatgct
     3181 ctttttctcc cataaaggcc caaacctgat tgccaaaatc ataatgccac cattcatgtg
     3241 gataattagt aaagtctgca ctaatcatcg tattatataa tagtcttctg ttctttaaga
     3301 tatttttttc tttttgattt aactgtcttt tattttttaa cctttcataa taatcggtat
     3361 gggtttcacc ggttgtttga tcaaaaccgg tccccatacc cagttctttc cccttgttat
     3421 caataatggt taaatcaact gccccacccg ttatatgagg agaaggacta tccatcttat
     3481 gagaaggcaa agctacatac tcttccactt tttcttttag tatatcctcc ccaatatccg
     3541 gattttctct ttttatctct tttttcagcc tatcaaaaag ctcatattga actgattcgg
     3601 gccgccaggc atcaaatata agaaacttat atccggccgg cagttgatcg gctgctttaa
     3661 gtaaattatc ataaacacct tccctaacat aacattcggg taatgaacct tcaataccct
     3721 ggataaagta ctggggtctg acaattattc gctcgggaat catatttaat gatactaact
     3781 tttcatttga actttttatc ttaatttctt ttactttttt ccaatcaaaa ttttctgtta
     3841 cgggtattgg cttttctagc attatttttg cggccattga atacctcctt ttaacctttt
     3901 tctcctaaaa gaattataaa ttaaatagat gtggtaaagt tgttaccagt tggggaaata
     3961 atattatcaa taagagtaca gtcattgcca ccgccacaaa aggccatatt cttctaaata
     4021 aggtttctaa tttaactttt ccgacagctg aaacaatgta aacacaggct cccattgggg
     4081 gtgtaattaa ggcgatcgta atatttaagg tcattactat tccaaaatga agggggttaa
     4141 ttcccgcata catggcaact ggagccacaa caggtgttaa taatattaag gcagctattt
     4201 cttccataaa catcccaatt acaaagatca taagtgagag gataatcagt aataccgtat
     4261 tattatcagt cagggttaac atatagcccg caaacttttg cggaacctgt tcccatttta
     4321 aaagccagct gaatacagca gcactgccca ttatcacaaa aacaacactt gtattacgaa
     4381 ctgtttcatt tacggaagct gcaaagtctt ttaaggataa ttttttggta acaaaaaatc
     4441 ctacaatcgc agtgtaaaat acggcaacag ccccagcttc agttggggtt acaaccccgg
     4501 taatgattcc ccctataata attatgggag cgatcaaggc aattaatgat tcccaggctg
     4561 ttttcagtat ctttttaatg ctagcccttt catttgaaac tggataatta tatttttttg
     4621 aatagtaata gttcattatc ataaaagctc cacctaacag caaacctggt atgatacctg
     4681 ctacaaatag gcctccaata gattgatttg tcatggaacc ataaaggacc ataaaaatac
     4741 tggggggtat tattggacct ataagtgaac ttcctgctgt aattcccgca gcataaggtc
     4801 tatcatatcc ctctttagcc atggcgggaa ttaaaataga acctatcgct gaggtgtctg
     4861 ctgcggcgga gccactgata ccagcaaaga tgatacttgc tactacattc acatgtccga
     4921 gcccaccttt aaggtgtcct actaagaccc ggctgaaatc cagtaattta tctgtaatac
     4981 cggtcctatt cattaagttt ccggtcataa taaacagggg tattgctaat aaagtgaaaa
     5041 tatccatacc gccgaaaaac tgctggacca ttatcctaaa aagagaaaaa tctcctaagt
     5101 ataaaaagcc gaaaaatgaa gatattaata aggctataaa gataggtacg ccagctaata
     5161 atagtattaa aaatgatatg accattaata tactcattct ctcatctcca ttttatcttc
     5221 tgtattaatt cctaaaattt caactaaaat atactgtact aaaaataagc ccatgccaat
     5281 tggaatcgac attaagggga ttgctttagg tattcctaaa gctaaagtct ggaatctcca
     5341 ggcattatca acataatgaa tacccaaata tgtcatatat ccaattatac cgatgatcaa
     5401 aagatgtact ataaaattaa tgattttact tataatatca ggtaaatatt ctaaaactat
     5461 agtaatttta acatgatctc caaaactcaa agcataaaca gaaccaaaca taactgaata
     5521 aatcaaagta aacctggcca cttcttctgt ccagaccagg gaatcattga gaaaataccg
     5581 aaagaaaata gaaattaaaa tatctgaaat attgattaca agaaataccc cagcaagaac
     5641 catgctgagg tattttatat aattcgatat tttcttcaat aacccattcg atatcgaatt
     5701 attgttcatc tttattttgc ctccatgtta gccttttcag cactttctaa agcttctttt
     5761 acataactag catcgatttt atcttcaatc cagctaatat aatcaggctg gccgatttcc
     5821 tggaactgct tcatttcttc ttcagtcgga tagtaaacct gcataccatt ttcttctagg
     5881 aagtcaaccc tttcactaac cttagcctct acactaattc tattcctaat attagcggct
     5941 ttagcggcct gtcttattac atactgatct tctttcggta aagaatttaa ccaatcagca
     6001 ttgatggtca accactggca ggaatactgg atattagcca ttgtcacata atcctgtact
     6061 tcatataaac taccagaaat tatataagtc gggggattca tctgaccatc tactacaccg
     6121 gtttgtaaag ccatgtataa ttctgtccag ggaatgggag ttccacttgc cccaaaggat
     6181 tcatacagag caatttgacc gggatccatg cctctaaact tcaatccttc aaaatcacta
     6241 ggtgattgga tttccttttg gttattggta aatgctaaaa aaccaccttc ttcgaccgct
     6301 tccaggagag ctacacctgt tttttcttca aattctgcct gggtcatctc ccagtactca
     6361 ctgctatcaa agaacttatg ggctgcttca aaactttcaa acataaaagg tattgtggcc
     6421 gcataaatct caggatagac agaaaccata ccggcattag aagcaacatt aatcttggta
     6481 gaaccactca tggtctgttc cattctctgt tcttcattac ctaattgact atcagaataa
     6541 agagtaattg taattctgcc attagtaccc atttcaacaa attctttaaa gttcacagca
     6601 tattcatgta cagcatttgt ttcaacatca gcaggaccat tataggacat tttaacattg
     6661 atactttcct gagctcctac tacaccaact gctgaaaaga ttaataatga caagactaat
     6721 aataaaatga gtttcttaga cattaatttt tgtccccctt ttttttattg aaataaattt
     6781 atatttattt tattcaacac gatattaaaa aatcctttat attttttcat aataataaaa
     6841 ttaattttaa tattatgatg attttattga attagtgctg gatttattct ataatataaa
     6901 ataacataag aatttgaggt gcagaaaatg aaaattggca agaaaataaa aaagtatcga
     6961 aagttaagaa atattactat ctcagaatta gcggaagaag ctgatttaag cgtgggcatg
     7021 atcagtcaaa tcgaaagaga taagattggg ctatctgtat catctttatg gaaaatcgcc
     7081 caggctttaa atgtatcaat cggagacttt ttcgaagaag aaaccccggg cgataaatat
     7141 gttgttagaa aagataacag gaaaaaaata aaagttactg actctacagc aacctatgaa
     7201 ttacttaccc ctgatttgtc ctggaatatt gagtttttaa agataactat tatgccgggg
     7261 gaaagcactg ataataaaaa aatctcccat aagggagaag aaactggtta tataatcaag
     7321 ggtaaattgt tgattaaatg gggagaaaaa gaatttatct taaacaaagg agatagtatt
     7381 cgattagata gtaccatacc ccataggtac attaatatcg gagatgaaaa atctgtatct
     7441 atttgggcca tgactccccc cagtttttaa agttattttt cttcactttc cttataattt
     7501 tcataaaatc tatggcttcc atcacagaat ggtttatttt tagaagcacc acagcgacag
     7561 agtgaataat gttcttcaca ttcgggcgca tggtcccggt ctgatttgag ttttattccc
     7621 ccctcaatat caaatgggcc atttttgcca taagggccgg gcgaaatccg gatcttcttt
     7681 ctttggttgc ctactttttt cacccgttct cctcccggta aactgtaact taaagctcca
     7741 gaaggacata gttcacatat tcttataata tcctttgcat cagccgcatc tggatctatc
     7801 catttaaatc tagtcttatc aaataccttt ggcaataaat ttgtgcagta accaatatga
     7861 ctacaaatac ccctgttatc ataaattgta atgttttcac cttcatactt aattacctta
     7921 tctgaaactc tatcttcttt tttctcatca tcaaaatcta catccatatg ggaaccatca
     7981 caaaaagggt ggtcatcaga atgaccacat ctacataaag acatccttga agaagtttcc
     8041 atctcttctc ccttactatt aagcatcaac ttcacattat atgcaatata tggtccgtcc
     8101 tttattatct caataacctc atcatcatat ttgctcatta tttgaacctc cttctcgttc
     8161 ttccacttat taactttatt tttctttatc aatttattat ttcctttttt cctaattgct
     8221 ttttttaaca ataataatga aaatttgtaa cttcaatatt ttattagagc atagcgaatc
     8281 ccaataataa tcctataata attccagcat ttgcaaaatg gttatgaata atattggatt
     8341 gggggattaa ttcgtcatta actatataga tcattgcacc ggctgcaaag gctaaaaaac
     8401 ctcctacaaa aacttttgtt atattaaaga ttaacaaact aataaaagta cctactacgg
     8461 tcattaatcc cgctcctaaa gcaaatataa taatctttaa atttttaatc ccagccgttt
     8521 ttaaaggacc tgcgatagct aaaccttccg gtacattatg aagggctatt gcgatggcga
     8581 tatataagcc tatctcagga ctcgattcaa aaccagcccc gatggctatc ccttccggca
     8641 ggttgtgtaa tccaataccg aaaaatatta aataacccgt ccttaacatt gataggttca
     8701 tttttatttt ttcggggttt tctatttcaa gatggtccgt tgttgataaa tgagaatggg
     8761 gaatcaattt atctaaaccg aacatcatta aggcaccaaa aataaatgaa attattgaaa
     8821 tcgtcataga actcaatact accgcttccg gtaacaattc aaaaacagag atggccagca
     8881 taattccccc tgcaaagcct aataacattg ctaaaaactt ctcttttggt ttaccaaaga
     8941 ttaataaaac aactactccc agggcagtcg aaactcccgc tataaaacta tataatagtg
     9001 ctcccataaa tttttactcc tttatataat atcagtttct tttttattac cagcctttac
     9061 ccagtggcaa taatattctt ctattaaatc tataccttga aaaattaaca gtcctaaaat
     9121 gctgatagct aaaattccac taaacatatt aacataatta accatgaccc agctgttcat
     9181 tatataatat cctatcccat actgggtagc aaagttttcc gcgtaaaata aaacagctat
     9241 actggtacca actgtaattc taagtgctgt taatatctgt ggtaagatag ccgggattat
     9301 tacatgttta taagtctgta taccttttac tcccacggaa cgggcggaat aaaaatattc
     9361 gggattaata tttttaacac tgtctcttgt agttacaatt atttgaaaga ttacaattac
     9421 catgataagg ataatctttg atctatctcc cagaccaaac aacaacataa ataatggtag
     9481 aaaggcaatt ttaggtatgg gatatattaa gtagataacc ggggaaatat actggtcaac
     9541 cttatcatta atcccaacca acagtcctat tgcaaccccg atcagtaaag ctaatattac
     9601 tgccgttatt attctatata agctcattgc tagatgaggt agtaagacag gaattaatat
     9661 atcaaaaaag ttaataataa ctgtcagggg agaaggcaca atactagaat taaagataat
     9721 atgtaatata ccccagaaaa taataatgaa caaggctcca taaaatgtat ttaacctttt
     9781 acttttaatc acaataaatc atcctcagac atcatagttc ttaatttttt acaaagttta
     9841 aagaactctt ctttttctct taacttgata tctccaaaat agggattatt aatttgatgg
     9901 ataaactctc cctctttcat tactgcaatt ttctggccta aaaagaccga ctcatctatg
     9961 ttatgtgtaa caattaaata ggacagatta ttttcctgat gaatttttaa gattaaattc
    10021 tgcatttctt ctctggtgat tgcatccaaa gcagaaaatg gctcatccat taataacaaa
    10081 tcaggcttta agaccagtga gcgggcaact gccactcgtt ggctctgacc cccgcttaat
    10141 tcagccggga atttatccct gaatttatat atacttagtt tttttagaac ttgaaataca
    10201 gtattttcga ttttatcttt atcataatct ctaacctgca gacctaaagc aacattatta
    10261 aaaacatttt tccagggaaa caatccataa ttttgtaaga taacccctgt ttccttcctg
    10321 atgctattga tgattttatc atcaattttt atttt
//
LOCUS       DATGCJ010000263        17987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_478120, whole genome shotgun sequence.
ACCESSION   DATGCJ010000263 DATGCJ010000000
VERSION     DATGCJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478120"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VKN64_11595"
     CDS             complement(<1..470)
                     /locus_tag="VKN64_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMA60920.1"
                     /translation="MTIKKGLIFGLILLVIITSSIIFFNNYLNGQANNSNKTIELNEI
                     DKESRVNKLLYNRQSVRNYNNQSISFKNISEIIWSAEGINVDGISGPTRTSPSAGATD
                     PLVIYVSISKVSNLKSGLYRYEPTEHLLHYLIEQDISEPLAEAALGQSPVAEAPA"
     gene            complement(701..2011)
                     /locus_tag="VKN64_11600"
     CDS             complement(701..2011)
                     /locus_tag="VKN64_11600"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HMA60921.1"
                     /translation="MNYFDEIEDRFEEAEFLQKSTNKVKIKIENWDLESIEHNNLIST
                     SLRVKEDSTFGSNYTYGKSSEIMEKLIKGAEKSIKFGKEANFCFSSDRIDKSSLKRRE
                     EYENTTPAVLFDYLQDIMVYIKEKQPDLTLDIDLVKLFDSITMKTSQGADLKDEISNY
                     RLYIGSPVPGGGSQLIRGFESESMFTDLFKEEIDELLAEYNIAREVSVPKTGKLPVLF
                     SPRSLYFVLVSLQAGLSGKKIYQETSPLIDKKGEKIFSDKIDITDKPHLVKSGSRRFF
                     DDEGILTSQSEIIKEGILKNYIYDLEYADKLDAKPTGHGLKKELFSADISTSVSPNFI
                     NPVIEPKDKSKNELLSEIDKGIYVTNVIGFHSSNYEQGHFSVQAQGFNVENGKLVGRL
                     QDVMIAGNIYEDFNNVIGVGDKIYSNMWGYAPYILVDKVSVTGE"
     gene            complement(2013..3386)
                     /locus_tag="VKN64_11605"
     CDS             complement(2013..3386)
                     /locus_tag="VKN64_11605"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMA60922.1"
                     /translation="MKERFTKLLSKDHLDYGDIRFEKNNETDIRYEGNELKDIKETYR
                     EGGHIRIYDEGSKVYGSFSEVEDAVQTFGELQKLSNVAGDFQKEKISLKNSPVIKDKV
                     KINPQHHPRDISLSDKKDLLKKYNELALKQDNVINTIFHYKDFNSKRIFVNSEGTVIE
                     YELLASYIHGAIFAKKGDVVQIKGVSFGGFPEFNNLLNREEDLLEKVEILQKMLAAEP
                     VKAGTYPIIVNPQLAAVLIHEAFGHLSEADIIKNNPSFIDKLQMGKKLGSEILNVIDD
                     PTIKNTPGYYKYDDEGQPGIRTELIKDGILNGRLHSRETANIFSEPVTGNMRAVDCNH
                     TPIIRMSNIFIDSGDDNLQDMINSIDKGYYLFNSKGGQTTGDQFTFGAEYGYKIVNGE
                     KQEMVRDINISGELFKTLRNITMVGDDLTFNEVGGCGKGDPMQLNIFSGKGAPHIKIA
                     EANIGGR"
     gene            complement(3456..4025)
                     /locus_tag="VKN64_11610"
     CDS             complement(3456..4025)
                     /locus_tag="VKN64_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMA60923.1"
                     /translation="MELKKNKLIAEALKVATKAHRGGNRKGGNQIPYIVHPIEVALTL
                     QENDMPEEIIAAGLLHDTLEDTTIKVETLEDRFGREITELVIGASERLENRDQNSWEK
                     RKQYTVDHLKEAEKKIKYISCADKLSNIRSMIRDYDKIGDELWSRFNRGYEKQKWYYI
                     NLVDSLSELKGMEMYEQFRLAVDYLFRNN"
     gene            complement(4146..4595)
                     /locus_tag="VKN64_11615"
     CDS             complement(4146..4595)
                     /locus_tag="VKN64_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMA60924.1"
                     /translation="MIDEIDYTILDELEDDGRVSYAELGRKVDLTRASVRERIVKLKN
                     EGVIEKFTVVLNPEKIEKGLSAFFEIDVEPNHLDKVATRLAEEERVYSLYLMTGASTL
                     HMHSLLSDQDELEDFMISKIYSQKGITRVKSNIILKRYKSREGGARL"
     gene            complement(4638..4835)
                     /locus_tag="VKN64_11620"
     CDS             complement(4638..4835)
                     /locus_tag="VKN64_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60925.1"
                     /translation="MKNNLFDLKEIFAEKDRNRGLLWSVMITWINFPFLYWGVTSNNY
                     MVITLGLFIAAVGTGFTLYFG"
     gene            complement(4870..6438)
                     /locus_tag="VKN64_11625"
     CDS             complement(4870..6438)
                     /locus_tag="VKN64_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HMA60926.1"
                     /translation="MEFELNFFYWFLSFIPIVAVLTLIMGFRWSGGKSGAFSWFAAAV
                     IGYLFFGANLKLLAVASLKGLWTTIFILYIIWGAMALYNIVSKINGFDVIARTFTGLT
                     GKTKVLQLLVISWGFVSLLQGVAGFGTPVAVAAPLLVGLGFSPVIATSSALLGRAWSV
                     TFGSLGSSYAALLKMTNLDPGGLAFWSSIFLTIVCIVTGLSISYLYNGKEGLKKGAPA
                     ALAIALSMGLTLIVTANFVLPYIASLVAGLVGMGVGTLILPKTRWYNNNPVKKKKSKN
                     TMSFHTAFAPYYALLILAFGIYLTPLKSMLSKFEIGLSFPRTETALGVVNEATASYSP
                     IAILTTPGTLVFLSVVIAIIYFIKKGLWKKHYGREVLGSLIKQAIPSTVTVMTMSMMA
                     VVMMETGMTSFLAKGTAEIASSVFPGVSPFIGILGSFMTGSNTSSNIVFAAFQKDVAL
                     FLGINSLIIASLQTTGGAIGTIISPMNVALGTGVTKIVGKEGEIIRKTIFYVIIMGLS
                     VGIPALFLIYYLGF"
     gene            6661..7611
                     /locus_tag="VKN64_11630"
     CDS             6661..7611
                     /locus_tag="VKN64_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HMA60927.1"
                     /translation="MRRKVLSIFILTSILVVGVFGIVTAQDYPTKPVQFIVPWSPGGG
                     SDTLMRIVAKYLEEYIGEAVPVINKPGVSGTLGLKELKEKPANGYHIGQIHEGLLAAH
                     HVGITELNYNDFTPVASMTNSPQFLAVRADAPYSNLEEFIEYAKNNPGEISFGVTLKG
                     IPHIWTAILESKLETKFNYVGYEGTGERVQALAGGFIDAGPIDYASGIQFVESGKFKF
                     IAFAAEERSEKLPDLPTFAEHGYDIIWSVQRGIVVPQGTPQDVIDVLEEGLRKTADNE
                     DFVADVEKAGGEVKFRGQDNYINYLETLDKDIAAVADQIE"
     gene            7713..8177
                     /locus_tag="VKN64_11635"
     CDS             7713..8177
                     /locus_tag="VKN64_11635"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HMA60928.1"
                     /translation="MGELIVGILFLILSLIIFFQAGNLPDSSYEPLGPAGFPRIIVLA
                     MGILSIFLIVKKLKSMDLSQAGLDFREIFKEYKLIFITLVNFLIYILIMRYIGFRIST
                     FIFVFTTQLLLGPRKEKKYLIIGSIALVISLGSYYFFQNYLGVIFPAGIFFE"
     gene            8213..9715
                     /locus_tag="VKN64_11640"
     CDS             8213..9715
                     /locus_tag="VKN64_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HMA60929.1"
                     /translation="MIDMFLLGFEHILSFNLLLNMFLGVLAGIIAGAIPGFTITMAII
                     LTLPFTFAMSPTVGIATMLGVFVGGLSGGLITAALLGIPGTPSAVATTFDAFPMVRNG
                     QPGRALGIGIWASFFGSLLSMVLLITLAPQVANLAMKIGPWEYFSLIVFALTIIASVT
                     GDSVIKGLMAGLLGLLIATIGLDPLSGTARFTFGIGFLEAGVPFLTVLIGIFAVSRLL
                     SELENKEEVKQGEELVTGKIKFEIFTSLKEVLGQPINLIRSSLLGALIGAIPGAGASI
                     ANILAYDQAKKGSKHPEKFGTGIPDGIIASEAGNNSTAGGGLIPMIALGIPGSAVSAV
                     MLAALQIHGINPGPMLIINNPEIVGTIFVGFFIATIFMLLIQFWGARLFLKITHIPKY
                     ILVPTVMALCVIGSYLLNNRYSDLYILFVMGVIGYFLKKYNFSLAPVILGVILGPIAE
                     TNLRRAMMTSQDWTLFFTRPISLGFLILGLLSIMYSIWQNYSVKKKANNT"
     gene            9737..10501
                     /locus_tag="VKN64_11645"
     CDS             9737..10501
                     /locus_tag="VKN64_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HMA60930.1"
                     /translation="MAERYVLTEMSWPEVEKALKTVEIAIIPVGAHEQHGPNLAESCD
                     AVRAREFSKLAAERLYPYVVVTPTINMGISYHHMPFPGTITLRPETLIELLRDICNSL
                     KKHGLKKFLFLNGHGGNSTTLDIATKILHEEENIEVAQVKYTDLAPESLAKYIHSEEF
                     GHSCEREVSEALYLIPEIVKTDSIEKSKTKKFPINNVSPVSGGPFTMGYSWDQKSING
                     ALGDATQASKEIGKAICDEALDNLAKFIEEFMAYKK"
     gene            complement(10554..11156)
                     /locus_tag="VKN64_11650"
     CDS             complement(10554..11156)
                     /locus_tag="VKN64_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HMA60931.1"
                     /translation="MLKNEMGIKIKTNKPDNINRVKTSRSYCAFVGEARKGKTFYTFH
                     QDINCPLARFNLGLEKFNKEKLNNLASTLVAWNDAETRQKARHYLKKSHTLDYGEKYI
                     IYFPLNQAPDIEPDIVMKIGTPQEFMPIIREVTKLKGDWTESFISGVGGMCGEATAIP
                     LKKGKVNISLGCGGSRPHGKIAKDQLIMALPYKIYEKLNI"
     gene            complement(11179..12372)
                     /locus_tag="VKN64_11655"
     CDS             complement(11179..12372)
                     /locus_tag="VKN64_11655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HMA60932.1"
                     /translation="MFQKKLLQMVFNKVQGANFEVTYWDGQTDSFGDQPEQDSEFRII
                     FNEKLALNEIRKQPQLKLGEAYMRGDIDLEGNLKEVLKLGARNVNRLMEDAGDYIPDE
                     FWEHQKEATFEDQKEGVQDHYDLGNDFFKLWQDETMTYSCAYFKSKDDDLKEAQLQKI
                     DHILKKMNLQPGEKLLDIGCGWGSVAIKAVEQYGVKVVGITLSEEQVEGARQKVAEKG
                     LEDSIEIREQDYRDLAEKDVEFDKIVSIGMFEHVGQEHIPEYFEAVNKMLKEGGLSLL
                     HTITHPKEQPTNAWLEKYIFPWGYIPSLREVIWELPEHNFLVQDVENIGFHYSLTAEN
                     WYDNYEEVTDEVEEMFDEKFVRRWRLFLAGVVATFRFLDTSVHQILFTKGKNIELPLT
                     RDYMY"
     gene            complement(12535..13785)
                     /locus_tag="VKN64_11660"
     CDS             complement(12535..13785)
                     /locus_tag="VKN64_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMA60933.1"
                     /translation="MQDNQSSNNEFNWKVLMIITAAGFFAGVYRNGIMTLFPFLQAEF
                     DLTRAQVGLYSTFLFISMAVVVLFSGRIADYFGVKRSIFWGLSFMGVFITLHSLANGF
                     ILLMLFAMLNGLGLSIILPSTSKAVAEWFRGDNQATAMGIMTMGFALGGVAGASVLPW
                     LGVQLGWKMLVIILGGLFLIVGAVFYFTYQDKNNNSDQVKKDSNNSFLKNIGLFFNNK
                     YLVLLCVLGIIFGAVSGVVVTHFSFFLFTDYNFSEVTAGLGFMVLQVGSMVGRTGWGY
                     LNGKLLGGVERRGFFIVGILMSLVSLAFAFLSNFNPALPIIMVLAFLLGATGRGWHGL
                     YFSEVSDQVGEEKAGMGIGLSLLFIRIGIIVGPPIFGYIADRTGTYAYSWLLLGITSL
                     VSVVTLNYFLSKYHKDRERAYKRA"
     gene            complement(13981..14430)
                     /locus_tag="VKN64_11665"
     CDS             complement(13981..14430)
                     /locus_tag="VKN64_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMA60934.1"
                     /translation="MEKILLAVDGSEKSKQAADKLAEMVKSIESEVDIITVLESPDIK
                     LREVDAYIPDKTIEELREMNKEEIKEKAQKILKEAAEHFKDVDTKIKKIIKYGDPADV
                     ICDYAEENDIDMIVLADKGIGGIKRFFLGSITAKVVRHAKSSVMIVK"
     gene            complement(14736..15740)
                     /locus_tag="VKN64_11670"
     CDS             complement(14736..15740)
                     /locus_tag="VKN64_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60935.1"
                     /translation="MDKIDNLSKFLEENFPEKAEEIKDTLIELQEVMLSTNMEISESM
                     PDLMLDNKYNKARNFIDKSEDISNYIKKLEDIINLVSDENEEGSKPVYEEKDNKEPEL
                     EINKTPQMQEEAEIFLKEEEEKSILSEDKEENGEEEEKVKFEEDNDPKQEEVIEEINT
                     KEIRKQAEQEENKEESEPDKISASEISQMFEDQGDTYNNQENNVHYLDENFREKEPSA
                     FYIEEEGKEVESWSDMLVETCKLLKNRNPDKFVAFINDDDFKFISRSYFSYNKDQIKE
                     PEPMQLKNRKIYIETKLSPNVVNKLIQKLVNKYGIDQKSYKIYYKNDKSVSSEGKKIS
                     "
     gene            complement(16096..16599)
                     /locus_tag="VKN64_11675"
     CDS             complement(16096..16599)
                     /locus_tag="VKN64_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMA60936.1"
                     /translation="MTYIIINGAPGVGKSSLLNLLQDNLKSRYALIDGDDLARTVPLE
                     NNTEWLNLMQDNIAACSENFIKYGIKNIIISFVFPTQERLNRFKKLLKREQDNFLYIV
                     LKCEDDELRKRIKIRDKQRLLNIEDALNCNHKINRLENDYVVETTDKSPELVLSEILK
                     ILSEECQ"
     gene            complement(16671..17858)
                     /locus_tag="VKN64_11680"
     CDS             complement(16671..17858)
                     /locus_tag="VKN64_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HMA60937.1"
                     /translation="MRTIGAQVRGIRLPIIKEGDNLAEVVVKYLKRSWENEGYELKNQ
                     DVVGITESIVARAQGNYIEIDDIAEEINQKFTSDEIGIVFPILSRNRFSMILKGIARS
                     EKKIYLQLSYPNDEVGNPLIDEKAMVEHNFNPYSDTISEEEYYKYFGDYKHPFTGVNY
                     VDLYKEVAQEQNTEIEIILSNRAETILDYTKEVLAADIHTRFKTKSKLKEAGAETVLG
                     LDDICTEDKGKGYNKEYGLLGSNKASDTELKLFPKDGQELVNDIQNRLKDLTGKDVEV
                     MIYGDGAFKDPAAKIWELADPVVSPAYTKGLEGTPSEVKIKYLADNDLKHLEGEKLLN
                     SMKEEIKNKEEDLVANIKSQGTTPRRLTDLLGSLCDLVSGSGDKGTPVILIQGYFDNL
                     ADE"
BASE COUNT         5494 a         3530 c         2704 g         6259 t
ORIGIN      
        1 gccggggcct cagcaaccgg tgattgacct aatgcggctt ccgctaacgg ctctgatata
       61 tcctgttcaa ttaaataatg taataaatgt tcagttggtt catacctata tagcccactt
      121 tttaaattac ttaccttaga aattgaaaca tatataacaa ggggatcggt cgcaccggcg
      181 gatggggatg tcctggtcgg tccacttatt ccgtcaacat tgataccttc cgcagaccag
      241 attatttcac ttatattttt aaatgatata gattgattgt tataatttct caccgattgt
      301 ctattatata aaagcttatt aactctgctt tctttatcta tttcatttaa ctctatcgtt
      361 ttattagagt tattggcctg gccattaaga tagttattaa agaagataat tgaggaagtt
      421 attattacta aaagtatcag gccgaaaatt aatcctttct taatagtcat ccttctcatc
      481 tcctcacttt ataatatgaa aaaaccacca aaatttaaac atcttcttaa attaagtata
      541 taatataatg aactttaaac aaagtacata agctacagat tattgggtaa tataaaaaat
      601 aaaccctcat aaaagagggc ttaaaaattg attatttaat tttattcaaa tatttaaata
      661 gaaattaaga tataattttt ttagtctatc tgaactgtat ttattccccc gtcaccgaga
      721 ctttatcaac taagatgtaa ggtgcatatc cccacatatt agaatagatt ttatcaccaa
      781 ctccgattac attgttaaaa tcttcataga tatttccggc tatcattaca tcctgaagac
      841 ggcccactaa cttgccattt tcgacattaa aaccctgcgc ctgcacggaa aaatgaccct
      901 gttcataatt ggatgaatga aaaccaatta cattagttac atatatacct ttatctattt
      961 ctgataaaag ctcattttta cttttatctt taggttcaat tacgggattt ataaagttgg
     1021 gggaaactga agttgatata tcagcgctaa acaactcttt ttttagacca tggccggttg
     1081 gtttagcatc cagtttatcg gcatattcta agtcataaat ataattttta agtattccct
     1141 cttttattat ctcactctgg ctggtcaata taccttcatc atcaaaaaac ctccggctgc
     1201 ctgatttgac cagatgtggt ttatctgtaa tatctatctt atcactgaag atcttttctc
     1261 cctttttatc aattaaagga gacgtttctt gatagatctt tttaccactc aagccagcct
     1321 gtaggcttac aagtacaaag tataaagaac ggggtgaaaa taaaacgggt aatttcccgg
     1381 ttttaggtac agatacctcc ctagctatat tatattccgc cagtaattca tcaatctctt
     1441 ccttaaataa atctgtaaac atagactcac tttcaaaacc tctgattaac tgtgagcctc
     1501 ccccgggtac cggtgaaccg atatataacc tgtaattact tatttcatcc ttaagatctg
     1561 ccccctgact tgttttcatg gtaatactgt caaaaagttt aaccaagtct atatctaggg
     1621 ttaaatccgg ttgtttttct ttaatataaa ccataatatc ttgaagataa tcaaataaaa
     1681 cagctggtgt agtattttca tattcttctc gtcttttaag agaactttta tcaatacgat
     1741 ctgaactaaa acagaaattt gcctctttac caaattttat tgatttttct gctcccttta
     1801 taagcttttc cataatctca cttgacttac cataggtgta attactacca aaagtactat
     1861 cttctttaac tcttaaagaa gtacttatca gattattatg ctcaatactt tctaaatccc
     1921 aattttcaat ctttatcttt accttattgg tagatttttg taaaaattca gcttcttcaa
     1981 atctatcttc tatttcatca aagtaattca tcttatctcc cccctatatt tgcttcagct
     2041 atcttaatat ggggagcacc tttaccagag aatatattta actgcatagg atctcccttg
     2101 ccacaaccgc caacttcgtt aaaggtcaaa tcatcaccaa ccattgtaat atttcttaag
     2161 gttttaaata gttcaccact aatatttatg tctcttacca tttcttgttt ttcaccattg
     2221 actattttat aaccatactc ggccccaaag gtgaactgat caccggttgt ttgaccacct
     2281 ttactattaa aaagatagta acctttgtca attgaattaa tcatatcttg gaggttatca
     2341 tcgccactgt caataaaaat atttgacatc ctaataattg gggtatggtt acaatcaacg
     2401 gctctcatat taccggttac cggctctgaa aaaatattag ctgtttcccg agaatggagt
     2461 ctcccgttta aaataccatc ttttattaat tctgttctta taccgggctg tccctcatcg
     2521 tcgtatttat aataaccggg cgtatttttt atagtagggt catcaataac atttaagatt
     2581 tctgaaccta attttttacc catctgtaat ttatcaataa aagaaggatt gttttttata
     2641 atatcagctt ctgataaatg gccaaaagcc tcatgtatta gcaccgctgc caattgtgga
     2701 ttgactataa taggataggt gccggctttg acaggttcag cggcgagcat tttttgtaag
     2761 atttctactt tttccagtaa gtcttcttct ctattcaaca gattattaaa ttcagggaat
     2821 ccaccaaaac ttaccccctt gatttgaaca acatcacctt ttttggcaaa aatagcgccg
     2881 tgaatataac tggccagcaa ttcatattca ataacagtac cttccgagtt tacaaatatt
     2941 ctctttgaat taaagtcttt ataatgaaat attgtgttaa ttacattatc ctgttttaag
     3001 gcaagttcat tgtatttttt caagaggtct tttttatcag ataaacttat gtcgcgggga
     3061 tgatgttgtg gattgatctt tactttatct tttataaccg gagaattttt taaactaatt
     3121 ttttctttct ggaagtctcc agctacatta cttaatttct gtaattcgcc aaaggtttgg
     3181 acggcatctt ctacttcact aaaagaacca tatactttac tgccttcatc atatattctt
     3241 atatggccgc cttcacgata agtttctttg atatctttta attcatttcc ttcatatctt
     3301 atatctgtct cgttgttttt ttcgaacctt atatctccat aatccaaatg gtcttttgat
     3361 aataatttag taaatctttc tttcatcggc tcactccttt taaatttatt tatttaaatt
     3421 tttttatcta tccaaactat aaatgtaaaa acattttaat tatttctaaa aagatagtct
     3481 actgctaacc taaactgttc atacatttcc atacctttta attcacttaa actatcaact
     3541 aaattgatgt agtaccattt ttgtttttca taaccccggt taaacctact ccagagttca
     3601 tcaccaatct tatcataatc tcgaatcatg cttctaatat tacttaattt atcggcacag
     3661 gaaatatatt taattttctt ctctgcttct tttaaatgat ccactgtata ctgcttgcgc
     3721 ttctcccacg agttttgatc tctgttttcc agtctttcgg aagcaccgat aactaattca
     3781 gtaatttctc tcccaaatct atcttcaagt gtttctacct taattgtcgt atcttctaat
     3841 gtatcgtgta gtaaaccggc agcaatgatt tcttccggca tatcattttc ctgtaaggtt
     3901 aaggcaactt caataggatg cactatatat ggtatttggt ttccgccttt tctatttccg
     3961 ccgcggtggg ccttggtggc aacttttaaa gcttctgcaa taagtttatt cttctttagt
     4021 tccatagcta atcacatcct ttaaatacta ttatattaaa aaatagaaaa aatttctaga
     4081 ctttatgtta aattataaaa aaataacgct gacagaaaaa tctctttctg ccagcgttct
     4141 tgaaattaca accttgcacc accttcacga cttttatatc tttttaatat aatattactt
     4201 ttaacccgag tgataccttt ctgcgaataa attttactta tcatgaaatc ctctagttcg
     4261 tcctggtcac taagaagtga atgcatgtgt aaggtgcttg caccagtcat taagtataag
     4321 ctgtaaactc tttcctcttc agccaacctg gtggctacct tatcaaggtg gtttggttcc
     4381 acatctattt cgaaaaatgc cgataaacct ttttctattt tttctggatt taaaactact
     4441 gtaaattttt caattacacc ttcatttttc aactttacaa ttctttctct cacgcttgct
     4501 cttgttaaat caactttcct acccaattcc gcataactaa ctcgaccgtc atcttccagc
     4561 tcatctaaaa tcgtataatc aatctcgtca ataataaatc ccccctaatc ataatattcc
     4621 aaaccaaaat actatactta gccaaaatag agagtaaagc cagttccaac cgctgcaata
     4681 aatagaccca atgttataac catgtaatta ttagaagtta ctccccagta aagaaaaggg
     4741 aagttaatcc aggttatcat aactgaccat aaaagtccgc ggtttctatc cttttctgca
     4801 aatatctcct tgagatcaaa taaattattc ttcacaatat tcacccttaa tttctatagt
     4861 atgatctttt taaaatccta aataatagat taaaaataaa gcgggaattc caacagaaag
     4921 tcccataata attacataga aaatagtctt ccggataatc tctccttctt ttcctacaat
     4981 cttagtaact ccggttccta aagccacatt cattggtgaa ataatggtac caatcgcacc
     5041 accggtagtc tgtaaagaag cgattattag actattaatt cccaaaaata aggctacatc
     5101 tttctgaaag gctgcaaata ctatatttga tgaggtatta ctccccgtca taaaggaacc
     5161 caagatgccg ataaaaggag aaacacccgg gaaaacactg ctggctattt ccgctgtccc
     5221 cttcgctaaa aaggaggtca ttccggtttc catcatgacc actgccatca tactcatcgt
     5281 catcacggta actgtagaag gtatagcttg tttaatcaaa gaccccaata cctctcgccc
     5341 gtaatgtttt ttccataaac cctttttaat aaaatatata attgcaatta caactgataa
     5401 aaaaacaagg gtaccgggtg tagttaaaat agcaatcggg gaataagaag cggtcgcttc
     5461 attgacgacc cctaaggctg tttcggtccg ggggaatgaa agaccgattt caaatttaga
     5521 gagcatgctt tttaaaggtg ttaaatatat tccaaaagcc aagattagaa gggcatagta
     5581 aggtgcaaaa gcggtatgga aactcatcgt atttttactt ttcttctttt taaccggatt
     5641 attattatac catctagttt taggtaaaat cagggtcccc actcccatac caactaagcc
     5701 tgccactaag gacgctatat agggtaaaac aaagttagca gtgacgatta aagttaagcc
     5761 catgcttaaa gctatcgcta aagcagccgg agctcctttt tttaatcctt ctttaccatt
     5821 atacaaatag gaaatactta aaccggtcac aatacaaacg atggttaaga agatactgct
     5881 ccaaaaggca agaccgccgg gatcaagatt ggtcatctta agtaaggcgg cataagaaga
     5941 tcctaaagaa ccgaaggtta ccgaccaggc cctacccagt aaggcagaac tcgtagctat
     6001 gaccgggcta aaaccgaggc cgactaataa aggggcagca actgctaccg gggtaccgaa
     6061 accggctact ccctgcagta aggaaacaaa cccccaactt atgactaaaa gctgtaagac
     6121 ctttgtctta ccggttaaac cggtaaaggt tctggcgatg acatcaaatc cgtttatttt
     6181 agatacaata ttatataagg ccattgcacc ccagattata tataaaataa aaattgttgt
     6241 ccaaagtcct tttaaggaag caactgcaag tagttttaga ttagctccaa aaaataaata
     6301 tcctatcact gcagcagcaa accagctgaa tgcacctgat ttacccccac tccatctaaa
     6361 acccataatt aaggtaagta ctgcaacgat tggaataaat gataaaaacc agtagaaaaa
     6421 attaagttcg aattccatga cattatcttc ctttcaagca agcacaattt tttattagta
     6481 taacaaaata tgattttact gtcaatacta aaattatcca atgtatgaat ttaagcaatt
     6541 attttaatac aatcatttaa tttttagttc taaaagcttt acaattttat tttagtgtga
     6601 tatattaact gtattaatat ttatactgaa atataattaa atataaaaag gaggagttct
     6661 atgagaagga aagtattgag tatttttatc cttactagta tcttagtagt tggtgtcttt
     6721 ggaattgtaa ccgcacagga ttatccgaca aaacccgtcc aatttattgt cccctggtct
     6781 cccggtggtg gtagtgacac tttaatgaga atagttgcta aatatttaga agagtatatt
     6841 ggtgaagccg tgcctgttat taataaaccc ggagtaagtg gaaccctagg tttaaaggaa
     6901 ctcaaagaaa aaccggctaa cggttatcat ataggccaga tccatgaagg tctactggct
     6961 gctcaccatg ttggcattac agaactaaat tataatgact tcacacctgt agcatcaatg
     7021 acaaactcgc ctcagttttt agctgtaaga gcagatgctc cctacagcaa tctggaagag
     7081 tttattgaat atgctaaaaa taatccaggc gaaatctctt ttggagttac tttaaaaggt
     7141 ataccccaca tttggacagc tattttagaa tcgaaactag aaactaaatt caattatgtc
     7201 ggttatgaag gtaccggaga aagagttcaa gccttagccg gtggttttat tgatgcagga
     7261 ccaatcgact atgcttctgg tatccagttt gttgaatccg gcaaattcaa gtttattgcc
     7321 tttgctgcag aagaaagatc tgaaaaactg cctgatctac ctacctttgc agaacacggt
     7381 tatgacatta tctggagtgt ccagcgggga atagtcgtac cccagggtac tcctcaagat
     7441 gtaattgatg tattagaaga aggattaaga aaaacagctg ataatgaaga ctttgtggcc
     7501 gatgtagaaa aagcaggtgg agaagttaaa tttagaggac aggataatta tataaattac
     7561 cttgaaacac ttgataaaga tatagctgct gtagcagatc aaattgaata atagttaatt
     7621 tgaaatgaaa acaaagaaaa aagctgtggg ggacttgatg atcaatcccc tgcagcctat
     7681 atttaattaa taatttaaag gagtcttaga ttatgggtga attgattgta ggcatacttt
     7741 ttttaatatt gagtctaata atatttttcc aggctggcaa cttgccagat tcgagttatg
     7801 aaccacttgg ccctgccggt tttcccagaa ttatcgtgtt agcgatgggg atactatcta
     7861 tttttctaat tgttaaaaaa ttaaaatcaa tggatttaag ccaagcagga ttagatttta
     7921 gagaaatatt taaggaatat aagttgattt tcataaccct ggttaatttt ttaatctaca
     7981 tacttatcat gaggtatata ggttttagaa tttcaacctt tatattcgtc tttactaccc
     8041 agctattact ggggcctaga aaagaaaaaa aatatctcat aattggctcg attgccctgg
     8101 tgatcagttt aggttcatat tattttttcc aaaattatct gggagttatc ttcccggccg
     8161 gtatattttt tgaataaaaa ttatttgaat acttataaaa agagggattt ctatgataga
     8221 tatgttttta ttaggatttg aacatatatt aagctttaac ttattattaa acatgttttt
     8281 gggagtactt gccggtatca ttgccggagc tattcccgga ttcacaatta cgatggcaat
     8341 tatactaact ttacccttta cctttgccat gagccctaca gtcggaatcg caacaatgtt
     8401 gggagttttc gtaggaggtc tttcaggtgg tttaattact gctgcccttc taggtattcc
     8461 tggaaccccc tcagcggtag caactacctt tgatgctttt cccatggtaa gaaatggcca
     8521 acccggtagg gctcttggta ttggtatttg ggcttccttt tttggttcct tactgagcat
     8581 ggtactatta ataaccctgg cacctcaggt agctaattta gccatgaaga tcggtccctg
     8641 ggaatacttt tcccttattg tctttgcctt aactataatt gccagtgtaa cgggtgattc
     8701 agtaattaaa ggattaatgg caggactttt aggtctttta atcgcaacca tcggtcttga
     8761 ccccttaagc ggtactgccc gttttacctt cgggatcggc tttttagaag caggggttcc
     8821 ctttttaaca gttttaatag ggatctttgc cgtctcaagg ctactatcag aactagaaaa
     8881 caaagaagag gtaaaacagg gagaagaatt agtgacaggt aaaattaaat ttgagatttt
     8941 cacttcctta aaagaagttt taggacagcc tataaatttg ataaggtctt ccttgcttgg
     9001 agcacttatc ggagcaatac ccggagctgg agcaagtatt gctaacatac ttgcctatga
     9061 ccaggctaaa aaaggttcga aacaccctga aaaatttggt acaggtattc ctgatggaat
     9121 tatcgcttca gaggccggca ataactccac tgccggtgga ggtctaattc ccatgatagc
     9181 tctgggtatt cccggaagtg cagtaagtgc tgtaatgcta gcagccttac agattcacgg
     9241 tattaaccct ggtcccatgt tgattatcaa caacccggaa atcgttggta ctatctttgt
     9301 cggctttttc attgccacaa tttttatgct tcttattcag ttttggggag caagattgtt
     9361 tttgaaaata acacatattc caaagtatat tctggtacca acagttatgg ctttatgtgt
     9421 aattggttcc tacctcttaa ataatagata tagtgacctt tacatcttat tcgtaatggg
     9481 ggtaatagga tatttcctca aaaagtacaa cttttcttta gcacctgtta tcttgggagt
     9541 tatattgggt ccaattgctg aaactaattt aagaagggcc atgatgacaa gtcaggactg
     9601 gacactcttt tttacaagac caatctcact cggcttttta atcttaggct tactttcaat
     9661 aatgtattcc atctggcaga actattctgt caagaaaaaa gcaaataata cttaaactat
     9721 taaaggggtt gaaataatgg cagaaagata tgtattaacc gaaatgagct ggccggaagt
     9781 agaaaaagct ttaaaaactg ttgaaatagc aattatcccg gtcggtgctc atgaacaaca
     9841 cgggccaaat ctagccgaaa gttgtgatgc agttcgggcg cgggagttca gtaaattagc
     9901 agcagaaaga ctttaccctt atgtagttgt aaccccaact attaatatgg gaatttcata
     9961 ccaccacatg ccctttcccg gcactataac attgaggcca gaaacactga ttgaattgtt
    10021 gcgcgatata tgcaactcac tgaaaaaaca tgggcttaaa aagttcttgt ttcttaatgg
    10081 ccacggggga aattccacaa ccttagatat cgccacaaaa attttgcatg aggaagaaaa
    10141 tattgaagta gctcaggtca agtataccga cctggctcca gagagcctgg caaaatatat
    10201 ccattcggaa gaattcggtc actcctgtga aagggaggtt tcggaagctc tttatttaat
    10261 accagagatc gtgaaaacag acagtattga aaaaagtaag actaaaaaat ttcctattaa
    10321 taatgtgtct ccggtaagcg gtggtccttt taccatgggt tatagctggg atcaaaaaag
    10381 tatcaatgga gccctgggag atgccaccca ggccagtaaa gaaattggaa aagcaatctg
    10441 tgatgaggcc ttagataatt tagccaaatt cattgaagag ttcatggctt ataaaaaata
    10501 attttaaaaa cagattaaat gggacagcgg attatcggct gtcccttttt gtattaaata
    10561 ttaagttttt cataaatttt ataaggtaaa gccataatta attgatcttt agctatttta
    10621 ccgtgcggcc tgctgccacc gcatcccaag gaaatattta ctttcccttt cttaagagga
    10681 atagcagtag cttcaccaca cattcctccc acaccgctta taaagctttc tgtccaatca
    10741 ccttttagtt ttgttacttc tcttattatc ggcatgaatt cttgcggagt cccaattttc
    10801 ataactatat ccggctctat atcaggtgct tggttaagag gaaaatatat tatatatttt
    10861 tcaccataat ctaaggtgtg agatttttta aggtaatgcc gggctttttg tctggtctcc
    10921 gcatcattcc aggccaccag ggtagatgct aaattattta acttctcttt attaaatttt
    10981 tctaacccta aattaaatct ggctaaagga cagttaatat cctgatggaa agtataaaat
    11041 gtcttgcctt ttctagcttc tcccacaaag gcacagtaac ttctgctcgt tttaactcgg
    11101 tttatattat ctggcttatt agtttttatt tttataccca tttcattctt taacatatca
    11161 tattacccct aataattatt aatacatgta atctcgagtt aaaggtaatt ctatattctt
    11221 accttttgta aataatattt ggtgaacaga agtatcaaga aagcggaaag tagcaactac
    11281 tcctgccaga aatagtctcc atctccttac aaacttctca tcaaacatct cttcgacttc
    11341 atcggttact tcttcataat tatcatacca gttttccgct gtcagtgaat aatggaatcc
    11401 gatattttct acatcctgta ctaaaaaatt atgttcgggt agttcccaga taacttccct
    11461 gagagaaggt atatatcccc aggggaaaat atatttttcg agccaggcat tagtgggttg
    11521 ttctttgggg tgagttatcg tatgtagtaa ggatagaccg ccttctttta acattttatt
    11581 gacagcttca aaatattcgg gaatgtgctc ctgaccaaca tgttcaaaca ttccgatact
    11641 tactatttta tcaaactcaa catctttttc agctaaatct cgataatcct gttccctgat
    11701 ctcaatacta tcttctaaac ctttttcagc tactttttgg cgagcaccct ctacctgttc
    11761 ttcacttaag gtgatgccaa ctacttttac tccatactgt tctaccgctt taatcgctac
    11821 tgaaccccat ccacagccaa tatcaagcag tttttctccg ggctgtagat tcattttctt
    11881 cagaatgtgg tctatctttt gtaattgagc ttcttttaga tcatcatctt tagatttaaa
    11941 ataggcgcag gaataagtca tagtctcatc ctgccaaagt ttaaagaaat cattacctaa
    12001 atcataatga tcctgtacac cttctttttg atcttcaaaa gtagcctctt tttgatgttc
    12061 ccagaattca tccgggatat aatccccggc gtcttccatt aaccggttaa catttctggc
    12121 ccctaacttt aatacttctt ttaagtttcc ttccagatcg atatcgcctc tcatgtaagc
    12181 ttcgccaagc ttcaattgag gctgcttgcg aatctcattt aacgcaagct tttcattaaa
    12241 gatgattcta aattctgagt cttgttcagg ctgatctccg aaactgtctg tctgaccgtc
    12301 ccaataagtt acctcaaaat ttgctccttg tactttgtta aataccatct gtaatagctt
    12361 cttttgaaac atatttatta ccccctattt agatattagc ttaaatcatt aataatcaat
    12421 aatttgctaa aaggaataat agagataatg gtctattgaa aataaatctt atatattact
    12481 ttaaatatta aagtgtaata ccctttttta ttttttaaca aatgttcatt aaaatcaggc
    12541 tctcttataa gccctttccc tatctttatg gtattttgaa agaaagtagt tcaaagtaac
    12601 tactgaaacc aaggatgtaa tacctaataa cagccaactg tatgcataag tgccggtcct
    12661 gtcagctata tatccaaaga taggcggacc cactataata ccgattctaa tgaataaaag
    12721 agaaagtcca atccccatcc ctgccttttc ttctccgacc tgatctgata cttcagaaaa
    12781 atataggccg tgccagcccc gcccggtcgc tcccaggaga aaagctaaaa ccatgataat
    12841 gggtaaagct ggattaaagt tacttaaaaa ggcaaaggcg agagaaacta gactcatcaa
    12901 tatacctaca ataaaaaagc ctcttctttc aactccaccc agaagtttac cgtttaaata
    12961 accccagcct gttctgccga ccatactccc gacctgtaat accataaaac ctagtccggc
    13021 tgttacttca gaaaaattat aatcagtaaa taaaaagaaa ctaaaatgcg taactaccac
    13081 accagatacc gctccaaaaa ttattcctaa aacacataat aataccaaat atttattatt
    13141 aaaaaataaa ccaatattct ttaaaaagga attattggaa tcttttttaa cctggtcaga
    13201 attattattt ttgtcctgat aggtaaaata aaagactgct cccactatta gaaataggcc
    13261 gcctaatata atgactaaca tcttccagcc taactgcact cctagccagg gcagtacact
    13321 ggcgcctgcc actcctccca gggcaaaacc catggtcata attcccatgg cggtggcctg
    13381 gttgtctccc ctaaaccatt ccgcaactgc tttactagtc gacggtaata tgatgctcaa
    13441 tcctaagcca tttaacatag caaataacat cagtaaaatg aagccattgg ccagggaatg
    13501 aagggttatg aatactccca tgaaagataa accccagaat atactgcgtt taacaccaaa
    13561 ataatcggca atccggcccg aaaaaagtac tactacggcc atactgataa acagaaaggt
    13621 ggaatatagt cccacctgag ctctcgttaa atcaaattca gcctgtaaaa agggaaataa
    13681 ggtcattata ccatttctat atacaccagc gaaaaatcca gctgctgtta ttatcattag
    13741 aactttccaa ttaaattcgt tattactact ttgattatct tgcattatgg acactccttt
    13801 agcatcaatg tattaagaat taaatatata atatttttat tatgcctatc ttagattatc
    13861 ttattataac agttatcagc atcattataa acaaaataat aacatatttt taagcttctt
    13921 tatgtaaaaa aataacccct cgaatgaggg gcaatttatt tatatatatt aaattatatt
    13981 ttattttaca atcattacag aactttttgc atgtcttact acttttgcag ttatacttcc
    14041 taagaaaaat ctttttattc caccgatacc tttatcagcc aatactatca tgtcaatatc
    14101 attttcttcc gcataatcac agattacatc agctgggtct ccatacttta tgattttctt
    14161 tattttggta tctacatctt taaaatgttc tgcagcttct ttcaatatct tctgggcttt
    14221 ttcttttatt tcttctttat tcatttctct taactcttca attgttttat cagggatata
    14281 tgcatctact tctcttaact ttatatcagg tgattccaat actgtgatta tatcaacttc
    14341 actttcaatg gacttgacca tttctgctaa cttgtcagcc gcttgtttag atttctccga
    14401 accatctact gccaatagaa tcttctccat gatacccctc cttttcgttg ctttttgtta
    14461 ttataaaata aatattatga cggggtacat tttttatatt atttcttctt acaatataat
    14521 tatatctaat tttacagaat ttttccataa atattttggt tatttcaaaa taatttattt
    14581 gaatatattt tttctttaca acatctccct ttatatcttt ttacctttgt tgacttttat
    14641 tcaatactat ctcctataaa gtaatctccc attctgagaa aaaaaataac cccgataaag
    14701 gggctaatat ttgtagaaat ttatgtaatt ttattttacg atattttctt tccttctgat
    14761 gatactgact tatcattttt ataatagatc ttataacttt tttggtctat accgtattta
    14821 ttgaccaatt tttgaattaa tttattaacc acattgggac ttaacttggt ttcaatatag
    14881 atcttcctat tttttaactg cattggttct ggttctttaa tttgatcttt gttgtaagaa
    14941 aaataggaac gactgataaa tttaaaatcg tcatcattaa taaaggccac aaacttgtca
    15001 gggttcctgt tttttaataa cttgcaggtc tctaccaaca tatctgacca gctctctact
    15061 tccttacctt cctcctcgat ataaaaggca gaaggctctt tttctctgaa attttcatca
    15121 agataatgaa cattattctc ctgattgttg taagtgtccc cctggtcttc aaacatctgg
    15181 ctgatttctg aagcactaat tttatctggc tcagattcct ctttattttc ttcttgttca
    15241 gcttgttttc tgatttcttt tgtgtttatc tcctcgatta cttcttcctg ttttggatca
    15301 ttatcttcct caaattttac tttttcttcc tcttctccgt tttcttcctt atcttctgat
    15361 aatattgatt tctcctcttc ttctttcaga aatatttcag cttcctcttg catctgagga
    15421 gttttattta tttctaattc aggttcttta ttatcttttt cttcataaac aggtttagac
    15481 ccttcttcat tttcatctga aactaagtta ataatgtctt ctaacttttt tatgtaattt
    15541 gaaatatctt ctgatttatc gatgaaattt ctagccttat tatatttatt atccaacatg
    15601 agatcgggca tcgattcact aatttccata ttagtgctca acataacttc ctgtaattcg
    15661 attaaggtat cttttatttc ttcggctttt tcgggaaaat tttcttctaa aaacttggat
    15721 aaattatcta ttttatccat tttaacacct ccctttaggt gaaaagtata caaagaatct
    15781 atattaaata cttaattttt aactattttc tctagtacta tatataatta gagataaatt
    15841 gatattctcc ttctttatat aataattatt caaaaaatga ggttatttgc cacctccaac
    15901 agttatttac aaaaagggct tttttattta aaatgatatt tctaataata ttaggcaact
    15961 ctaaactttc tctactcaat tataataatc gatagatatc aaataatatt aaacgaaaac
    16021 aagttctctc tctgctaatt atctctttat tacttgttta gaaaactctt ttctatgtat
    16081 tataatgaat ttgtgttatt gacattcctc acttaatatc ttcaaaattt cagaaagtac
    16141 caattcggga cttttatcag ttgtttccac aacataatcg ttttctaatc tattaatttt
    16201 atggttacaa tttaaagcat cctcaatatt taataatctt tgcttatctc ttatctttat
    16261 tctttttctc aattcatcgt cttcacattt gagaacaata tacaaaaaat tgtcttgttc
    16321 tctttttaat aactttttaa acctgttcag cctttcctga gtaggaaaaa caaaagaaat
    16381 tataatattt tttattccat attttataaa gttttctgaa caggctgcta tattatcctg
    16441 cattaagttt aaccattcgg tattattttc taatgggact gtcctagcca aatcatcacc
    16501 gtcaattaaa gcatatcgac tcttaagatt atcttggagt agatttagta aagaagattt
    16561 tccaactccc ggggctccat tgattattat ataggtcatt attacacctc aaaaaattta
    16621 taataaaaac cctgccccaa ggacagagtt ttcttgatta caactttcta ttattcatca
    16681 gctaggttat caaagtaacc ttgaattaag atgaccgggg tgcctttgtc accactacca
    16741 ctgaccaagt cacataaact acccagtaaa tctgttaatc ttctgggggt agtgccttgt
    16801 gattttatat ttgctacaag gtcttcttcc ttgtttttaa tttcttcttt cattgaattt
    16861 aatagttttt caccttcaag gtgtttaagg tcattgtcgg ccaggtattt aatttttact
    16921 tccgaaggtg tcccctctaa ccctttggta taggcgggtg agaccaccgg atcggctaac
    16981 tcccaaattt tggcggccgg gtctttaaaa gcgccgtctc catagatcat tacttctaca
    17041 tctttaccgg tcagatcttt caacctgttt tggatatcat taaccagttc ttgtccatct
    17101 tttggaaata atttaagttc tgtatcggaa gctttattag aacctaaaag tccgtattcc
    17161 ttattataac ctttgccttt atcttctgta caaatatcat ctagtccaag tactgtttcg
    17221 gctcccgctt cttttaactt acttttggtt ttaaaccggg tgtgaatatc ggctgctaaa
    17281 acctcttttg tataatctaa aatggtttca gctcgattcg aaagaataat ctctatctct
    17341 gtattctgct cttgggctac ttctttatat aaatcaacat aatttacacc ggtaaaaggg
    17401 tgtttatagt ctccaaagta tttataatat tcttcttcac ttatcgtatc tgaataaggg
    17461 ttaaaattat gttcgaccat ggctttttca tctattaagg gattaccaac ttcatcgttg
    17521 ggataactta actgaaggta tatcttcttt tcacttcttg caattccttt taatatcatg
    17581 gaaaacctat ttctgcttaa aataggaaaa acaataccta tttcatcaga agtaaatttt
    17641 tgattgattt cttccgcaat atcgtcaatt tctatgtaat tcccctgggc tcttgcaact
    17701 attgattctg taataccaac tacatcctgg ttcttcaatt catatccctc attttcccat
    17761 gaccttttta aatatttgac cacaacttcg gccagattgt ctccttcttt aataatggga
    17821 agtctgatac ctcttacctg tgcaccaatt gttcgcatat cctacactcc tctgttattt
    17881 ttttatctat ctttaaaaaa gataatgtta ataatcttct atataaaatt attattaatc
    17941 ttctttagtt attaaattga tatcgcttaa actgattaat aaataaa
//
LOCUS       DATGCJ010000264         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_267291, whole genome shotgun sequence.
ACCESSION   DATGCJ010000264 DATGCJ010000000
VERSION     DATGCJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267291"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..703)
                     /locus_tag="VKN64_11685"
     CDS             complement(173..703)
                     /locus_tag="VKN64_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HMA60938.1"
                     /translation="MRKKLKGIDLELVKGDIAAQEDIEAVVNAANAQLKIGGGVAGAI
                     HRAAGPELEKACRSKAPIQTGEAVITDAFNLPNDYVIHVLGPVYGRDKPEEKLLSNCY
                     ENALNLAEKNEIKSIAFPAISTGAFGYPIAEATEITLNTVNDHLDNLDSIKKIRFVLY
                     GQSDFKVYKEKLNEIL"
     gene            complement(912..1604)
                     /locus_tag="VKN64_11690"
     CDS             complement(912..1604)
                     /locus_tag="VKN64_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60939.1"
                     /translation="MECLKDIFGKFLEEQKERLAKRTFNKYSDVMDLFEKYLDGYAYQ
                     YLDEEEIESYKQQKFNEDTDFCEIYSIDKIKGYLLDDFMTYFMIKKVWGSKELMKNTG
                     TVLRKFVKWLKENSYIEEEKFNDLYDLVNEKKDDLPKVEELSELLYDEGIRNSIYNYD
                     EFEEEYFVITSIKKGELWLESFSAENKKVGPVKVSNEISSLAQVGWNVYLVLGQSGGS
                     WFIIESGNVYPD"
     gene            complement(1633..1755)
                     /locus_tag="VKN64_11695"
     CDS             complement(1633..1755)
                     /locus_tag="VKN64_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60940.1"
                     /translation="MEGQFEYIAIYTSGGIPFGIKKEDNDIDKIKELPFETNKE"
     gene            complement(1964..2446)
                     /locus_tag="VKN64_11700"
     CDS             complement(1964..2446)
                     /locus_tag="VKN64_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60941.1"
                     /translation="MELTVKLEDVLEAIELHTMESNYFYYKKDGKVYYISDEELRAAE
                     DDYDLEDFPEWQHDSIKRAEDIISTMDYIRLPDRHDINDYDIMEAFCLSIEDDELSEI
                     MYDSIKGSGAFRRFKENIHKYGIADDWYDYQEYIYIEIAKDWCKEHGIKYIEGDRNNG
                     "
     gene            complement(2492..2989)
                     /locus_tag="VKN64_11705"
     CDS             complement(2492..2989)
                     /locus_tag="VKN64_11705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HMA60942.1"
                     /translation="MSLGKFEINQIGIICQDDEEFFIKLGRRYRGGLQSLEGFSHIQL
                     LWWAHLADTSEDRKKLLFGKIFKQGPDQMGVFSTRAPARPNPIMSSIIKIKSIDYKEG
                     IIHTSFIDAEENSPVLDIKPYYPMERVKECQVPIWCQKWPAWYEETIGFDWKDVINFR
                     QDKPV"
     gene            complement(3036..>3558)
                     /locus_tag="VKN64_11710"
     CDS             complement(3036..>3558)
                     /locus_tag="VKN64_11710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMA60943.1"
                     /translation="TQMENEELDVEYKVQDFLTLEYEEEFDVVLLLYCELGALTNEER
                     NKLLNVIYRALKPGGLFIFDVFTAQNRDKDDLGKSWDLAQKGFWDEKPYLSLTETFFY
                     TEVDTYLNQTIIVNEEEEISLYRIFEHFYNKETISEVLEQFGFINHAFYSDLTGQKYT
                     PKSKTLAVVTEKL"
BASE COUNT         1086 a          742 c          419 g         1311 t
ORIGIN      
        1 tatttataat attattaacc aaaaaacata tcttttaaag ttatactact ataatattgc
       61 ttaaagtttt atttatccct tcctaataaa gaaaaatatc ctcctaaata ctaaataaat
      121 agcatttaag aggaatttaa aagcaattac atatcttcaa ttttagagct tattataata
      181 tctcattcaa tttttcttta tatactttaa aatcactctg gccgtacagg acaaatctta
      241 tcttctttat gctatctaaa ttatctaaat ggtcattcac tgtgtttaaa gtgatttcgg
      301 ttgcttctgc tattggatat ccaaaagcac ctgttgaaat tgctggaaag gctattgatt
      361 ttatctcatt tttttctgct aaattcaagg cattttcata gcaattgctt aaaagttttt
      421 cttcaggttt atctcttcca tatacaggtc cgagaacatg gataacataa tcattgggta
      481 gattaaatgc atctgtgatt actgcttcac ccgtttgaat aggggctttt gatcggcagg
      541 ccttttctaa ttcagggcca gccgcccggt gaattgcgcc tgctactccc ccacctattt
      601 ttaattgggc attggctgca ttgactaccg cctcaatatc ctcttgagca gcgatatctc
      661 ccttaaccag ttcaaggtcg atccctttta acttctttct cataggaata cccccttggt
      721 ctaatttatc tttgtcacga gtaatgactt taccaagaaa gccaataacc agcccaataa
      781 ccactgccaa aacaattgaa taaaaccagc tatcaatttg taaagataat gaagcagcta
      841 aaacagcact taatactatg acaattatta gcttattctt aataatatat catccttccc
      901 gataattaat attaatctgg gtagacattt ccgctctcaa tgataaacca acttcctcca
      961 ctttggccga ggactaagta aacattccaa ccaacttgag ccagtgagct aatttcattg
     1021 gataccttta ctggccctac ttttttgttt tctgccgaaa atgactctag ccagagttcg
     1081 ccttttttta tactagtaat tacaaaatat tcctcttcga attcatcata attatatata
     1141 ctattcctaa taccctcatc gtataagagt tcggaaagct cttctacttt aggtaaatca
     1201 tctttctttt cattaactaa atcatataaa tcattaaact tttcctcttc gatataagaa
     1261 ttttccttta accatttaac aaatttcctt aatacagtac cagtattttt cattaattcc
     1321 ttacttcccc aaactttttt gatcataaaa taggtcatga aatcatccag taaataaccc
     1381 ttgattttgt ctatagaata aatctcacaa aagtctgtat cttcattaaa cttttgctgt
     1441 ttataagatt caatttcctc ttcatctaaa tactgatatg cataaccatc taaatacttt
     1501 tcgaaaagat ccatcacatc actatattta ttaaaagtcc ttttagccag tcgctctttc
     1561 tgttcctcta aaaattttcc aaaaatatct tttaggcatt ccaagagata agcctccttt
     1621 gtatttaaaa tattattctt tatttgtttc aaaaggaagt tctttgattt tatctatatc
     1681 gttatcttct ttttttattc caaaaggaat cccgcccgaa gtatagatag ctatatattc
     1741 aaactggccc tccatatagg atttaggccg attatctgcc tcaaatatat cctcattttt
     1801 ttcagtatca aaatttccta accttgttaa ctcagcagag acggcaaaga gatcaaactc
     1861 atatctttcc caataagctc tttctatatc tttgccttcc tattcagtaa tccattctcc
     1921 cacatcttct aatctttctt tttcattata gttctttagc tatttagccg ttatttcggt
     1981 ctccttcaat atattttata ccgtgttcct tacaccagtc tttggctatt tctatataga
     2041 tatattcttg ataatcatac caatcatctg caatcccata tttatgaata ttctctttaa
     2101 atctcctaaa ggccccactt cctttaatag aatcatacat tatctcactt aattcatcat
     2161 cttctataga aagacaaaat gcttccatta tatcataatc attaatatca tgacgatcgg
     2221 gtaatcgaat atagtccata gtagatataa tatcttccgc cctttttata ctgtcatgct
     2281 gccactcggg aaaatcttct aaatcataat catcttcagc agctctcaat tcctcatctg
     2341 atatataata aaccttacca tcttttttgt aataaaaata attgctttcc attgtatgca
     2401 gttcaatcgc ttccaaaaca tcttctaact ttacagtaag ttccatcttc tccccccata
     2461 ttttaagtgt tttttatgcc attattatta actatactgg cttatcctgt ctgaaattaa
     2521 taacatcttt ccaatcaaaa cctatggttt cttcatacca agccggccat ttttgacacc
     2581 agataggaac ttgacattct tttacccttt ccattggata gtatggttta atatctaaaa
     2641 caggtgagtt ctcttctgca tctataaatg aagtgtgaat tatcccctct ttatagtcga
     2701 tactcttgat ttttatgata ctgctcatga ttggattggg gcgggccgga gctctggttg
     2761 aaaatacgcc catctggtcc ggaccttgtt tgaatatttt accaaataat aattttttcc
     2821 tgtcttcgct ggtatcagca agatgagccc accataaaag ttgtatatga gaaaaccctt
     2881 ccaggctttg caaacctccc ctatatcttc gacctaactt tataaaaaac tcctcatcgt
     2941 cctgacatat aatcccgatc tgattaattt caaacttacc caaggacata aaatcccctt
     3001 ctttctcata ttttttatct tatttattat tataattaaa gtttttcggt tactacggct
     3061 aaagttttag atttaggagt atatttttgg ccggtcaaat cagaataaaa agcatggtta
     3121 ataaaaccaa attgctctaa tacctcacta atagtttctt tgttatagaa atgttcaaat
     3181 attctatata aagatatctc ttcttcttca ttaactataa tagtctgatt taaatatgta
     3241 tcaacctccg tatagaaaaa tgtttctgtt aaagataagt aaggtttttc atcccagaaa
     3301 cctttttggg ctaaatccca gcttttacca agatcatctt tatctctatt ttgggccgta
     3361 aaaacatcaa atataaataa gcctccgggt tttaaagctc gataaataac attaagtaac
     3421 ttattacgtt cttcattagt taaagcccct agttcacaat acagaagtaa tacgacatca
     3481 aactcttctt catattcaag tgttaaaaag tcctgtactt tatattccac atctaattct
     3541 tcattctcca tttgagtt
//
LOCUS       DATGCJ010000265         6134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_267427, whole genome shotgun sequence.
ACCESSION   DATGCJ010000265 DATGCJ010000000
VERSION     DATGCJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267427"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VKN64_11715"
     CDS             <1..1191
                     /locus_tag="VKN64_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HMA60944.1"
                     /translation="LYNQLKDMEKELELYEKIRLLYVAMTRAEDKLILSGYTKNNRKL
                     TFEDSKSWLDWLATYLDKESFDKSEEICFNYDNEALKLKVNIIEDEKFKTDLDIKQKE
                     ISEDIIINDWDKQAEWEKISDKISPIEIESKEHKAEISVTGLMEYERCPRLYYLRHIK
                     RIPDLDALKAKSDKIQYNENTIDPLKKGLIIHRLYELSGLEEEPAKYIDQVLTEMNYE
                     ELDQGKKADLIDILKSYQTREKYYLDKKADLSKDYHEKRFTLNFPNFFLKGTVDNIYL
                     YQDGEIEIIDFKTNNIKKDEVKTTSEDYKMQLEAYSLAVSSLFNKSKIKYRIEYLIPE
                     VNYSKILNRGELNSIRAKIKELGNKIAVSNRKEDFNTIADQNCRYCIYGQLCGKKNNI
                     TKEY"
     gene            1206..2069
                     /gene="galU"
                     /locus_tag="VKN64_11720"
     CDS             1206..2069
                     /gene="galU"
                     /locus_tag="VKN64_11720"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HMA60945.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPAIQYIVEEA
                     VQSGIEDIIIITGKHKKSIADHFDKNIELEMALKEKQKAELLEIVEDISSMIDIHYVR
                     QKKPKGLGHAIYCARTFIGDDPFAVLLGDDIVVSEKPVIQQMAEVYEREEKPIIGVQK
                     VPHMDISKYGVVKYKNSKDRLYEVEELIEKPSVEEAPSDLAIMGRYIITPDIMEILAN
                     TKPGKGGEIQLTDALNELAHRREVQAYEFEGDRYDVGNKLGFLQATVDFALDRGELGE
                     KFRKYLKKIIK"
     gene            2093..3202
                     /locus_tag="VKN64_11725"
     CDS             2093..3202
                     /locus_tag="VKN64_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60946.1"
                     /translation="MKKLAVTLLAVMFMLTFTFNTAATDITAKSYGMGGAYTGLADDA
                     TAMLYNPAGLSDSGFMGLQFEFGMGASNIDKMSELIGDIEYFAEDFDVNSPEDIKDIN
                     ELFNDFPIINVDNQIFAGGNFSSFGVGFNIDNRFDTNKPSDEIINATNITNTSGILSI
                     SRDLNTDYKEIIGISYGVNIKTLRTDYVEVELVNSATNKKASVATAKGSGVGLDAGVL
                     VNFTPKLKFGANFKNILAPDYDLEGEKEVYDYLAAEGANTDTSNFTKTYSPKQSYRLG
                     ASLDIPVINGTVVGDIENYTDSDKSVIHLGVEKRLMFNGLSLRGGMYNPSDEDPTLTM
                     GLGFNLASLHFDMAIGSNDGFSINLNGAASLNFQF"
     gene            3503..4354
                     /locus_tag="VKN64_11730"
     CDS             3503..4354
                     /locus_tag="VKN64_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60947.1"
                     /translation="MKKYLILMLMLVMVFTLSNVGTAYYDETMTYYDSVINTGDVVNL
                     GNVVGYDIRLSDSSPLHLLEARYYFNDDTIVDFQTYSFSNSTFTENFNFNLGYEFLNK
                     NNWKMKSNLYRNHNGDYFTSIYTDKKMNKKLTLHNNFKVGFYDNYTGKSFATGLTYNV
                     NEKNDLRVGLSRGGYSEKFGELFENLFIRSALKTKVNKNVNNILSFEKGFNYDNLILR
                     EEIIYNPSDDIYLEGFFKLDTKADNNLGFEMQKGLTENVFVTAEYTKVLSDDSPSWIY
                     TGLIYKF"
     gene            4572..5135
                     /locus_tag="VKN64_11735"
     CDS             4572..5135
                     /locus_tag="VKN64_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMA60948.1"
                     /translation="MPKIIDNIEEKIYNAAFELFTNKGYDSVNMKMVAKKTGIAVGTL
                     YNYHQNKKQLYLDVFKNSWDDTYNKIDAILKEENRKDKLKSLINILYTDISKRRGFAQ
                     ELIKSNELKELGMKFVEELKNELKGKIKEALTAYIKYKKANISEDKKARLVQLIFTEI
                     IALNNEFSSEKEKNLVFIMELVNSTLE"
     gene            5191..5370
                     /locus_tag="VKN64_11740"
     CDS             5191..5370
                     /locus_tag="VKN64_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60949.1"
                     /translation="MIISFDPKLNGFLVEVKKKSDLFYQLIKKKSFELLENKNKFIIG
                     FIEYEDVEFDFQKED"
     gene            5510..6076
                     /locus_tag="VKN64_11745"
     CDS             5510..6076
                     /locus_tag="VKN64_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HMA60950.1"
                     /translation="MEILMVIFSYLFGSLSFAQVLANRKNMQLNKVGTKNPGAYNVYS
                     QISHSWGITSGAFDIFKGAFPVFIGRFIFNCDFTYLVLITAAAVMGHIWPIYFNFHGG
                     RGLATSLGTLFVFDFYLAFYALILAGLIGFWFRFLSDLEPRISLLMFPVFILSAYYFR
                     ANINLVIVGIVLMIVIYIRAIHLRYRTA"
BASE COUNT         2290 a          779 c         1149 g         1916 t
ORIGIN      
        1 ctctataacc agctgaaaga tatggaaaaa gagctagaac tttatgaaaa gataaggctg
       61 ctttatgtgg ccatgaccag ggcagaagat aagttgatct taagcggcta tacaaaaaat
      121 aaccgcaaac ttacctttga agacagtaaa agctggttgg actggctggc tacctatctg
      181 gataaggaaa gttttgataa gtcggaagaa atatgtttta attatgataa cgaggcacta
      241 aaactaaagg ttaatataat tgaagatgaa aaatttaaaa cagacctcga tataaaacaa
      301 aaagaaataa gtgaagatat tattataaat gattgggata agcaagcaga atgggaaaaa
      361 atttcagata aaatttcacc gattgaaatt gaaagtaaag aacataaagc cgagataagt
      421 gtgacgggtt taatggagta tgagaggtgt cccagactat attacctccg tcacataaag
      481 agaatacctg atttagatgc tttaaaagca aaaagtgata aaatacaata taatgaaaac
      541 acaattgacc ctcttaaaaa aggacttata atccaccgtc tttatgaatt aagtggttta
      601 gaagaggaac cggccaagta tatagaccag gtattaacgg aaatgaacta tgaagaactt
      661 gaccaaggta aaaaagcaga tttaatagat attttaaaaa gttaccaaac cagggaaaaa
      721 tattatttag ataaaaaagc agacctaagt aaagattacc atgaaaaaag gtttacctta
      781 aactttccta actttttttt aaaaggaact gtagataata tttatcttta tcaggatggt
      841 gagatagaga taattgactt taaaacaaat aatattaaaa aagatgaagt aaaaactaca
      901 agtgaagact ataaaatgca gttagaagct tactctctgg ctgtcagcag tctattcaat
      961 aaaagtaaaa tcaaatacag gattgaatat ttaattccag aagtaaatta tagtaagatt
     1021 ctaaaccgag gggaacttaa ttcaataaga gctaaaataa aagaattagg taataaaata
     1081 gcagtttcta atcgaaaaga ggattttaat actatagccg atcagaactg tcgatactgt
     1141 atttatggtc agctttgtgg aaagaaaaat aatataacta aagaatatta agggggtatt
     1201 taaagatgaa agtaaaaaaa gcagtaattc cagccgcagg tttaggaaca agatttttac
     1261 cagcaaccaa ggcccagccc aaagaaatgc tgccgatagt agataaaccg gccattcaat
     1321 atatagtgga ggaagcagtc cagtctggaa tagaggatat aataataatt accggtaaac
     1381 ataagaagag tatagcagat cattttgata aaaacatcga attagaaatg gccttaaaag
     1441 agaaacagaa ggcagaacta ttagaaatag ttgaagacat ttcatccatg attgatatcc
     1501 attatgtaag acaaaaaaag ccaaaaggct taggtcatgc tatttactgt gcccgaacct
     1561 ttataggcga tgatcccttt gctgttttgt taggtgatga tatagttgtt tcagagaaac
     1621 cggtcattca gcagatggct gaggtttatg aaagagaaga aaaacccata attggagtac
     1681 agaaggttcc ccatatggat atttcaaagt acggggtggt gaaatataaa aactctaagg
     1741 acagacttta cgaggttgaa gaattaattg aaaaaccgtc cgtagaagaa gccccttccg
     1801 atctagcaat aatgggtaga tatataataa cgcctgatat tatggaaata ttagcaaata
     1861 cgaaaccagg caaaggaggg gagatacagc ttacagacgc tttaaatgaa ttagctcatc
     1921 gtagagaagt tcaggcttat gagtttgaag gtgacagata tgatgttggt aataaattag
     1981 gtttcctaca agctactgta gactttgcac ttgaccgagg agaactagga gaaaaattta
     2041 gaaaatatct taaaaaaatt atcaaataat ttattaaaaa ggatgtgaaa caatgaaaaa
     2101 actagcagtt actttactgg cagtcatgtt tatgctgact ttcactttca acacagccgc
     2161 aactgatatt acagctaagt cttatgggat ggggggagca tataccggtt tagccgatga
     2221 tgcgactgcc atgttatata acccagctgg acttagtgat agtggtttta tgggtttaca
     2281 gttcgaattt ggcatgggag cttctaatat cgataaaatg agtgaattaa taggtgatat
     2341 agaatatttt gctgaggact ttgatgtaaa tagtccggaa gatataaaag atattaatga
     2401 actgtttaat gactttccaa ttataaatgt tgataatcaa atatttgccg gcggtaattt
     2461 ctcttctttt ggagtaggtt ttaatattga taatagattt gatacaaaca agccttccga
     2521 tgagattatt aatgccacta atataaccaa tacttccggt attctatcaa ttagccggga
     2581 ccttaataca gattataaag agataatagg gatctcatat ggtgtgaata ttaagacttt
     2641 aagaactgat tatgttgagg tagaattagt aaatagtgct actaataaga aagcttctgt
     2701 cgccactgcc aaaggtagtg gagttggatt agatgcgggc gttttggtga attttactcc
     2761 caaactaaag tttggagcga actttaagaa tattctcgcg cctgattatg atttggaagg
     2821 ggaaaaagaa gtatatgatt atttagcagc tgagggtgca aacactgaca cttctaattt
     2881 taccaaaacc tattctccca aacagagcta tagactgggt gcttcccttg atatacctgt
     2941 aattaatgga actgtggtag gagatattga aaattatact gattcagaca aatcggtcat
     3001 ccatttagga gttgaaaaaa ggctgatgtt caatgggttg tctttaagag ggggcatgta
     3061 taatccttct gatgaagatc ccaccttaac aatggggtta ggctttaatt tagcatccct
     3121 tcactttgat atggctatag gcagtaatga tggttttagc attaatttaa atggggctgc
     3181 ctcattgaac ttccagtttt aaataaaatt aaaatagaag taataaatcc tcaggtgtat
     3241 aatacatacc tggggttttt tatttaaatt agaaatagtt ggattatact attaaattca
     3301 ttaaaatgat atatagcact ccaataagat gtttctagtt atctaattaa tttattcact
     3361 atataagtac ctaatcctga taaatgtgta taaatctcat taatgataaa atatggtaaa
     3421 ttttgcagga atatagagtt taatacttaa tatagaaatt aatacacaaa ataatatata
     3481 aaatttagag gaggattagt aaatgaaaaa atatttaatt ttaatgttaa tgttggttat
     3541 ggtatttaca ctcagtaatg ttggtacagc ttattatgac gaaactatga cctattacga
     3601 ttctgtaatt aatacagggg atgtagtaaa tttaggaaat gttgttggct atgatattag
     3661 attatccgat agcagtccgt tacatctact agaagcaaga tactatttca atgatgatac
     3721 aatagttgat ttccaaacat attctttttc taatagtact tttactgaaa attttaattt
     3781 taatcttggt tatgagtttt tgaacaaaaa taattggaag atgaagtcaa atttatatag
     3841 aaatcataat ggagactatt ttacatcaat atatacagat aaaaaaatga acaaaaaatt
     3901 aacattgcat aataatttta aagtagggtt ttatgataat tataccggaa aatcttttgc
     3961 tacaggttta acctataatg taaatgaaaa aaacgaccta agagtaggat tatcccgtgg
     4021 tggatatagt gagaaattcg gtgagttatt tgagaattta tttataaggt ctgctttaaa
     4081 aacaaaagtg aacaaaaatg ttaataatat actgtccttt gaaaagggtt ttaattatga
     4141 caatttgatt ttacgtgaag aaattatcta taatccaagt gatgacattt atttagaagg
     4201 cttttttaaa ttagatacta aagctgacaa taatttagga tttgaaatgc agaagggttt
     4261 aacagaaaac gtatttgtta cagctgaata tactaaagtt ctttcggatg atagtccaag
     4321 ttggatatat acagggttaa tttataaatt ctaaatcata ttattaaata ttaatatatc
     4381 gacagggtgc ttttattaaa ttattagcgc cctgtttttt tatgtgataa attattaatc
     4441 aatatattta ggtaaatgat taagaaatag taaatctaat tagagttatt gtaatatttc
     4501 atctttttag attgacaata gatataatat ataataaaat gaattcaagt tcataaaaag
     4561 gagttgatat tttgcccaag attatagata acatcgaaga gaagatatac aatgcggctt
     4621 tcgagctttt cactaataaa ggatatgact ccgtgaacat gaaaatggtt gctaaaaaga
     4681 ctggtattgc agtcggcacc ttatataatt atcatcagaa taaaaaacaa ttatacctgg
     4741 atgtttttaa aaacagttgg gacgatactt ataataagat agatgcaatt ttaaaggaag
     4801 agaacagaaa ggataaatta aaaagcttaa taaatatctt atataccgat atatcgaaaa
     4861 ggagaggctt tgcccaggag ttaattaaat ccaatgaatt aaaagaacta ggcatgaaat
     4921 ttgtggaaga actcaaaaat gagttaaagg gcaagataaa agaagctttg acagcttata
     4981 taaagtataa aaaggcaaac atttctgaag ataaaaaggc ccgattggta caactcattt
     5041 tcacagaaat tatagcctta aataacgagt tcagttctga aaaagaaaaa aatttagttt
     5101 ttattatgga attagtaaat tcaactttag aatgaatttt tttgccattt aaatgaagtg
     5161 tagttcataa aattataagg aggtttttct atgattattt ctttcgaccc taaattaaat
     5221 ggatttcttg tagaagtcaa aaagaaaagt gatttattct accagcttat caagaaaaag
     5281 agctttgaac tgctggaaaa taagaacaaa ttcatcatcg gttttatcga atacgaagat
     5341 gtggagtttg attttcagaa ggaagattaa aaattagata aacaatcacc atatcccttt
     5401 aaatataatg ctcaatttca caaatttgca ggttttaata cttagtgata agatatatta
     5461 aaaggaaatt aataatattt cattaacaaa aggaatgtgg gtgttttgta tggaaatatt
     5521 aatggtgatt ttctcctatt tgtttggttc cctgtctttt gcacaggttt tggcaaatag
     5581 gaagaacatg cagctaaata aagtaggtac aaaaaacccg ggtgcctata atgtatacag
     5641 ccaaatcagc catagttggg gaataacatc aggtgcgttt gatattttca aaggcgcttt
     5701 tccagtattt atcggtagat ttatctttaa ctgtgatttc acctatctgg tattaattac
     5761 agcggctgcc gtcatgggtc atatttggcc gatctatttc aactttcatg gggggcgagg
     5821 acttgctact tctctaggta ccctatttgt attcgacttt tatttagcct tttatgccct
     5881 gattttagca gggttaatcg gattttggtt tagattttta agtgatctgg aaccaagaat
     5941 ttcactttta atgtttccag ttttcatttt aagtgcatat tactttaggg cgaatataaa
     6001 tctagttatt gtaggtatag ttttaatgat agttatttat ataagagcga ttcaccttag
     6061 atacagaact gcatagaaag gagatatatt atgacagacc gtgttttgac gattggaatt
     6121 gacggaggag aaat
//
LOCUS       DATGCJ010000266        19939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_354957, whole genome shotgun sequence.
ACCESSION   DATGCJ010000266 DATGCJ010000000
VERSION     DATGCJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 19939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19939
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354957"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..280
                     /locus_tag="VKN64_11750"
     CDS             107..280
                     /locus_tag="VKN64_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60951.1"
                     /translation="MPEIKEGNRSLQTESSRDELEVISANMPALEGPMLGEVWTEILR
                     ILSSCKTNNPAVL"
     gene            559..1830
                     /locus_tag="VKN64_11755"
     CDS             559..1830
                     /locus_tag="VKN64_11755"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HMA60952.1"
                     /translation="MKNNFKSIGAGSIQWLFFLLATMLTFPIIIGEIYHLSTIQIMGL
                     IQRTFFVVAITTLLQIKFGHKLPIIEGPTGIWLSIFIILAGLSSNQGAELHQVLKIIE
                     GGILTAGIILISLSALDLIKNLLSLFTPLVSGLYLILLPLQLSGTFIKGMLGINNQTL
                     EIDPVVAIISVSVFVLVLLLSFKAKGFLKSYAVLIGILLGWILFLIFGKALIINPKNT
                     VFALPSVFAWGLPDWNSGIILSTVIITLILVSNAVATMTAIGESIKKKTDSNTHKRGI
                     FMGGITHILSSLFSTAGGLVPLPITAGFISLSGHTQIMPLIIACIFLAVISFFPVLIG
                     YLALLPGPVAYAVSFASFIQIISIGFHTIFKNNLQQKHFTILGISLMFGIGIMFIPST
                     VINKLPLLVQYIGSNGLITGTMLCILLEQLT"
     gene            complement(2159..3373)
                     /locus_tag="VKN64_11760"
     CDS             complement(2159..3373)
                     /locus_tag="VKN64_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMA60953.1"
                     /translation="MLEKKRHTDHGQKWAVAVAMLDGAKTFLEIIEHFNKVKKRFGFF
                     VNETDYSESYFKSTLEELLDKGWAKKNEGRYCLTEVGKKEAEKAYSEIKRSREWINDY
                     LLNPTAVSKVTVIVHFVLALLKLPIGILSGSIGLLNDGVDTLLDGLSSVLVYFGIKYD
                     FEDIVNYILISIMFITGFYTLYQAVSRFFVPIENNIDWLTFTAVILSGLISLFLMLYQ
                     RYVGIKTGRFSLVTQSVDSRNHLIVAGSVVVGMLASLANFPIIDILVGVVVAGLILKG
                     AFDLVLEILDQDSEDSKYGMFKIGKNLYEERLKKYCLIKIEKERKSYNSWVQEIKEEL
                     DFTDNITLEGIGMQQAKYEVPQIKILLDSLIEEKLVEKDNDNLILTSQGKEKIKKAYK
                     FLNLKNIFNYFI"
     gene            complement(3426..3881)
                     /locus_tag="VKN64_11765"
     CDS             complement(3426..3881)
                     /locus_tag="VKN64_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HMA60954.1"
                     /translation="MIDKDLISKLDKIWHYFIFNFQNKKTGFWGEELKDATTIEVSIL
                     NIVDNNPEIMLKEIKEILDIPGSTLTSAVDRLEKRELIKRIISKRDRRSFGLELTEKG
                     KKAQKRHIRAEKELFNNILGALDSKEERKQFLNSLEKIKNYFNEQLKKG"
     gene            4083..4664
                     /locus_tag="VKN64_11770"
     CDS             4083..4664
                     /locus_tag="VKN64_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HMA60955.1"
                     /translation="MPQTVSDDKYKVWTRQHKNVLNELKNKGVYKVKERYIRKKLDTC
                     ADIYLDVYKWLRNQVSKRMDIPKKAKYPIWLTVEEDLKLPMADGYVFFELEIPKDKIM
                     VFDMAKWDYIVNYLYLPKNDEDRKRFKEKLDKYNISVESDIYLEDFYPMLKREMTSSW
                     ERLFDESIQLSDQKVGISWELKEEWVIDYEKRD"
     gene            4648..5208
                     /locus_tag="VKN64_11775"
     CDS             4648..5208
                     /locus_tag="VKN64_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HMA60956.1"
                     /translation="MKKEIDLWTQQSSIVKEKLLKEERTTVKKRYIKRKYKSEAEIFL
                     TAYNFFVKEAKKIINKPEDAEYPIWAAADRKNALSGGSGYLIKLKVPREKVILFDADK
                     WNKILNLKYLPDNEKDLKDHKKMLDMYGIDSDSDIILSPHYPKLKEKIKNSWQKLFED
                     DISNIENIKAGLWELRLEWVQDISEF"
     gene            complement(5308..5790)
                     /gene="rlmH"
                     /locus_tag="VKN64_11780"
     CDS             complement(5308..5790)
                     /gene="rlmH"
                     /locus_tag="VKN64_11780"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMA60957.1"
                     /translation="MNIILIAVGKMKEEYLLDGLEDYVERIKHYCNLDVIEIDPARSK
                     HKKNDAEIEKIKEDEGQRILAKVPEGKNYIIALDRKGKPVTSEGLAKSIKNLQVRGES
                     KITFLIGGSHGLSEEVLKKADYKMSLSYLTFTHQMSRLILLEQLYRAFKILNNEPYHK
                     "
     gene            complement(5808..6599)
                     /locus_tag="VKN64_11785"
     CDS             complement(5808..6599)
                     /locus_tag="VKN64_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMA60958.1"
                     /translation="MLVKVSVLSSGSSGNSIYIDDGKHSFLIDAGLSGKKIINRMKDI
                     DADPDDLDGIFVTHEHKDHIKGVGILSRRLDIPIYANEGTWAACENELGKLKSKHIKS
                     FKGDFSFGNFDIRPIGISHDARSPVAYVVYRNDKKMVFATDMGYINDELMEGLTDLDL
                     LLLESNHDIDMLMSGSYPRFLKRRIRGEKGHLSNDAAAEILPQLINGRCPQVLLGHLS
                     QENNNPKVAYLTVENILKKKDLVVDKDLKLDLTYQHKPTKIYKIK"
     gene            complement(6737..7384)
                     /locus_tag="VKN64_11790"
     CDS             complement(6737..7384)
                     /locus_tag="VKN64_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HMA60959.1"
                     /translation="MKDRDSIPAATIERLPLYYRCLDKLATFEETDVVSSKDLGGKLG
                     IPSTQVRKDLSYYGEFGRRGVGYNVKELKRSIGKILGVDKKWPAVLIGAGNLGRALIN
                     YAGFGKMGLEITDIFDNDLNKIGNRVGPIKVKSVNKLEEIIKEKDVNLAIVTVPADPA
                     QEVVDNLIDYGIKAIWNFSPTRLYVPDDVFVRNEDLAVGIVSLIYHLSWQMENEE"
     gene            complement(7406..9340)
                     /gene="metG"
                     /locus_tag="VKN64_11795"
     CDS             complement(7406..9340)
                     /gene="metG"
                     /locus_tag="VKN64_11795"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMA60960.1"
                     /translation="MDKDKTFYVTTPIYYPSDRLHIGHAYTTVAADSITRFKKMQGYD
                     AMFLTGSDEHGQKIERSAKEADKEPKEYVDEIVQTFKELWDALGVEYDYFIRTTEERH
                     EKVVQKIFQKLYDKGDIYKGHYEGWYCTPDETFWTERQVGEEKVCPECGRPLEWVEEE
                     SYFFKMSKYADKLLNHIKDHPEFIQPESRRNEMINFIEQGLDDLSVSRTTFDWGVPVP
                     IDDDHVIYVWIDALSNYITAIGYLTDEENFNKYWPADLHIVGKDILRFHTIIWPIILM
                     ALDLPLPKQVFGHGWLLSESGKMSKSRGNVVDPMELIDDFGVDPIRYYLLREVPFGTD
                     GTYSTEALIRRINSDLANDLGNLLNRTVSMVNQYFDGEIPEQSDLNEIDEDLMETGLN
                     TVDEVEEHMEDLRYTNALEELWNFVRRTNKYIDQTQPWILGREEGHEERLGTVLYNLL
                     DSLRMIAVMLKPFMVETPYEIGSQIGDVQQIIDADWSDLKWGQLKSNIKVRKAEPIYP
                     RIDMEEYFDKKQKKEQEKTMTNEKSNNEEELIDFESFQKLDLRVAKIIEAERIEDSNK
                     LVKLQVSLGEENRQLVAGIAKHYEAEDLVGKQIVIVANLEPAEIFGVKSNGMILAASN
                     EDELTLITPEKAIDNGSKVK"
     gene            complement(9359..10210)
                     /gene="rsmI"
                     /locus_tag="VKN64_11800"
     CDS             complement(9359..10210)
                     /gene="rsmI"
                     /locus_tag="VKN64_11800"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMA60961.1"
                     /translation="MSEKGKLYICGTPIGNLDDTTFRAVKTLEKVDLIAAEDTRRTEK
                     LLNHFKIDNNLTSYHEHNEREKAEKLITELKDGYDIALVSDAGMPGISDPGLILIKEA
                     IQAKIEIIPVPGPTALIPALVVSGIQTDKFSFFGFVPRKGKEREQFIEEIINEEKTTI
                     FYESPYRIKKIFEDLSEKIPERKAALIREITKIHEEKIYGTVSEIQDKIASSEIKGEI
                     VVVIEGRTKEEVDTESWTDISILEHVQLFMDNGYTKKEAIKKVAEVRGIQKKKVYKEA
                     IQINVNR"
     gene            complement(10203..10934)
                     /locus_tag="VKN64_11805"
     CDS             complement(10203..10934)
                     /locus_tag="VKN64_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMA60962.1"
                     /translation="MEEIHYLIPDELKIIQNDDFFKFGTDSVLLANYVEVKNNDKIID
                     LGSGSGVIPLLLAYKNNPDIIYGIELQSPLVEMSRRSVKLNGLEDKIEIVAGDIKEVM
                     SHFTDNEFDTVVTNPPYRTTDSGKITQNKYKAIARHEIEIDIKEIIDNTVRLLRAGGK
                     FYLVHLTKRLPQIFYFLKEKNLEPKEMRLIQSRANKDPDRFILKAVMDGKPGFKIKKN
                     IVEFAGDTDQYTDEVKKVYGVDKDE"
     gene            complement(10882..11253)
                     /locus_tag="VKN64_11810"
     CDS             complement(10882..11253)
                     /locus_tag="VKN64_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="initiation control protein YabA"
                     /protein_id="HMA60963.1"
                     /translation="MDEEILEELALFQEKIEEISMKFKNLKDITYKLYKENEELRNEN
                     EELKSIIFEEEEQQLPQSKNNLKRLHDEGFHICHLSFGEKRKGECLFCLRLLEDNNKQ
                     KGKSSGRDTLSNTGRTKNNSK"
     gene            complement(11266..12078)
                     /locus_tag="VKN64_11815"
     CDS             complement(11266..12078)
                     /locus_tag="VKN64_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HMA60964.1"
                     /translation="MEEVIGVSFKRAGKIYYFDPDNIEVEKGDKVIVETSRGIEMGEV
                     VLEKKKVAEKELVAPLKKVIRKATLKDIDKDEENKRLEDEAYDIALDKIKEHGLPMKL
                     IDVEYTFDHNKVIFYFTADGRVDFRELVKDLARVFKTRIELRQIGVRDEAKMIGGLGP
                     CGRRTCCDTWLRDFDPISIQLAKDQELALNPAKISGLCGRLMCCLKYESDYYEEITEK
                     MPEVGEKITLTNGSKAEVVDRNLIKQQVKLKNKDGDVIKFDLEEIKEIKEKN"
     gene            complement(12082..13074)
                     /gene="holB"
                     /locus_tag="VKN64_11820"
     CDS             complement(12082..13074)
                     /gene="holB"
                     /locus_tag="VKN64_11820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HMA60965.1"
                     /translation="MSFNNIPGQSKTKKILKDSIKKNRISHAYLFTGNNGLGKSEMAG
                     EFAQAIFCENNIADGCGECLSCKKIEHGNHPDFHKIMVEEDHKNILIDQIRDLQKEIS
                     YKPYESDHKVYIIDQAERMTIQAQNSLLKTLEEPPEYAIIILTNENKNELIPTIISRC
                     QEIKLHNQTTDIVEDYLINREGFNKEDAHLYATLARGKYKKAVKLAGKEDFVENRNRI
                     IEFIAGIESKNNFEIYNSADELIKLSDTEFPLFELILSFFRDILVMKRNGNNDIINFD
                     FKNLITKATENYSTEQLFELTKLINMYRDYYYRNIKKELLFPSLLIKIRNKKEL"
     gene            complement(13094..13441)
                     /locus_tag="VKN64_11825"
     CDS             complement(13094..13441)
                     /locus_tag="VKN64_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HMA60966.1"
                     /translation="MSESKTVKMVIAVVHDHDAHDLMEALSSSNFKATKMATTGGFLR
                     EGNTTFMIGTDEEHVDQVLEIIKENCQARKKTIAPMSPVANSMESYYSFPMDVKIGGA
                     TVFIMDVEQFIKM"
     gene            complement(13438..14064)
                     /gene="tmk"
                     /locus_tag="VKN64_11830"
     CDS             complement(13438..14064)
                     /gene="tmk"
                     /locus_tag="VKN64_11830"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMA60967.1"
                     /translation="MSGFFITFEGIEGSGKSTQMKLLTKYLRDLDYEVVFTKEPGGTK
                     IGEKIRKILLDPIHHKMDDRAEILLYAADRAQHVVETIIPAIEEGKIVISDRYIDSNI
                     AYQGHGRGLDMKMVREINEWVIRDYWPDLTILLDLDVREGLQRARDLTPDKSGDRLER
                     ELIEFHERVREAYLEMAEKEDRFKVIDASRAPEEIFQDVLKAIKEELL"
     gene            complement(14229..15158)
                     /gene="trxB"
                     /locus_tag="VKN64_11835"
     CDS             complement(14229..15158)
                     /gene="trxB"
                     /locus_tag="VKN64_11835"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMA60968.1"
                     /translation="MSDNIQKEKLIIIGGGPAGLTSAIYTSRAGLNPLVINGPEPGGQ
                     ITLTPDLENYPGFPDGVGGFEIMEKVTEQAEKFGTRMVFEIVEKVQFEEKPYIIETDS
                     TKYEAESVIIATGSEAKRLGLYREEELTGNGISYCATCDGAFFKGKEVAVVGGGNVAL
                     EEADFLTKFASKVYLIHRRERLRGDKILADRIKENEKVEILWNREVDELKGDKHLDGI
                     IIENNKTKERENLDIKGLFMAVGYDPRTELFKGQIELDEKNYIITDNKQRTNKEGIFA
                     AGDVQEPNFNQVVVAAGTGAKAAMQVDKYLLGY"
     gene            complement(15247..16710)
                     /gene="guaB"
                     /locus_tag="VKN64_11840"
     CDS             complement(15247..16710)
                     /gene="guaB"
                     /locus_tag="VKN64_11840"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMA60969.1"
                     /translation="MDKKFEKVALTFDDVLLQPAKSDILPKEVDVSTELSEDISLNIP
                     IISAAMDTVTESRLAIAIARQGGLGIIHKNMSIREQADEVDKVKRSESGVIVDPFSLG
                     PDDMIYEAEELMSKYHISGVPIVDNNYKLIGILTNRDLRFEQDFDRPIKEVMTKEGLV
                     TAPVGTTVEEAKSVLQKHKIEKLPLVDENFILKGLITIKDIEKARKYPNSAKDDNGQL
                     LVGAAVGTGKDTFNRVRAIFEAGADVVVVDTAHGHSTKVNDMVKDIKKKFPEITLIAG
                     NVATPEATEELIKAGADVIKVGVGPGSICTTRVVAGVGVPQVTAINEAAQMAKKYGKT
                     IIADGGIKYSGDIVKALAAGANTVMIGSLLAGTEESPGELEIYKGRSYKVYRGMGSVD
                     AMKKGSKDRYFQEGKKEEKFVPEGIEGRVPYKGKLSDTIYQLVGGLRSGMGYCGTADI
                     PALQKDAEFVRITSAGLRESHPHDVDITKEAPNYSID"
     gene            complement(16795..17397)
                     /gene="recR"
                     /locus_tag="VKN64_11845"
     CDS             complement(16795..17397)
                     /gene="recR"
                     /locus_tag="VKN64_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMA60970.1"
                     /translation="MKYYPGPVGRLIGELSKLPGIGQKTARRLSFYLLGRSKQEVKDL
                     ADAIIDAREKTKYCSICNNLTEQDPCEYCTSEERDQETICVVESPKDVIAMERTGEYN
                     GLYHVLHGAISPMDGIGPDEIEIKNLLPRLEEGVKEVIVATDPNAEGDATAMYIAKLL
                     KPLDVEVTRIAHGLPVGGDLEFADEITLSKALEGRREIKY"
     gene            complement(17398..17706)
                     /locus_tag="VKN64_11850"
     CDS             complement(17398..17706)
                     /locus_tag="VKN64_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMA60971.1"
                     /translation="MNMNKLMKQAQKMQAKMSEMQEELENKTYEATSGGGAVKALVNG
                     KKEVLDLQIDEDVVDPEDVEMLEDLILAAINKAMSEATEELNEKMGQMTGGMNLPGMF
                     "
     gene            complement(17734..19500)
                     /gene="dnaX"
                     /locus_tag="VKN64_11855"
     CDS             complement(17734..19500)
                     /gene="dnaX"
                     /locus_tag="VKN64_11855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMA60972.1"
                     /translation="MPSLSLYRKYRPQKFNEIIGQPAVTQTLLNAIENDRISHAYLFA
                     GPRGTGKTSTAKVLAMAVNCQEESTTEPCGVCDSCKRIKSGNSIDVIEIDAASNRGID
                     EIRELREKVKFYPGEGKYKVYIIDEVHMLTTGAFNALLKTLEEPPENVIFVLATTEPH
                     KVIPTIMSRCQRFDFSLISEKEIENHLEYICQEEDLDYEVAALNLIARSSQGGLRDAL
                     SILDQAVSYTGENIKTEIIEEMLGRTSGQELRELMMDLKEKNTQAAISLVNEILNEGK
                     DVDRLIEDLKEYCNQLLLIKECGIKTNLVKLPKERLSEMEKLAEKFSLSHLTKIVEVL
                     NEIKDEISLGGNSRINLEVGIFKMSDKLQEADLTQEVKELKEKVKYLENRITEGNITA
                     QAAVQSETDRETESEKEDDEVPSPEEQDYVKQTVETEQEEKAAKESKEEKQVSKEGLD
                     LHEVKKYWPQLLGNIKKEDISLHAVLVEGKPIKVVNDKVYISFPPDKNFHRKRAKEKK
                     EYIGRFLSSLFSRNLIIKIVSTEDNIEVDNNNQEKEQNDNQNKSAEDSMNKNSGDISV
                     EQVAKMFNGEIIETNPDIISNK"
     gene            complement(19545..19809)
                     /gene="ffs"
                     /locus_tag="VKN64_11860"
     ncRNA           complement(19545..19809)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKN64_11860"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(19841..19934)
                     /locus_tag="VKN64_11865"
     tRNA            complement(19841..19934)
                     /locus_tag="VKN64_11865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19896..19898),aa:Ser,seq:gga)
BASE COUNT         5853 a         4051 c         2891 g         7144 t
ORIGIN      
        1 gagttggacg atagaaagat taaaaaactt agtagcgtta ataatagcgg ctcgtaacaa
       61 tgaattagaa tttaagataa acaaaaggga ctggtaattt aaagagttgc ctgagataaa
      121 agaaggcaat cgcagtttac aaacagagtc ctcccgagat gaattagaag tgataagtgc
      181 taatatgccg gctcttgaag gaccaatgtt aggtgaagtc tggacagaaa ttctaagaat
      241 tttaagtagt tgtaaaacaa ataatccagc tgttctttaa attcaaaaaa attttaaagt
      301 ataaaaaaag tactttgagg tagtgtcttt aattagagag ggtttaaggg taaattaaat
      361 taaaattgat gtatctttat taaaaaattg tagacaatga aaaatgaggc taatttatct
      421 aatattaaaa ttcatttctt cttgtgagga agttgcccca caaaagtttg acaaaaactt
      481 ttttaaaatt atacttgaaa tttctataat caatgataaa attaattaaa tatctaattg
      541 gagtgaggtt ttaatataat gaaaaataat ttcaaatcaa ttggagcagg ttctatccag
      601 tggttatttt ttttattagc gacaatgctt acttttccaa ttattattgg agaaatatac
      661 catctttcaa ctatccagat tatgggttta attcaaagaa ctttttttgt tgttgctatc
      721 acaaccttgt tacaaattaa atttggacat aaacttccta ttatagaagg tccaactgga
      781 atctggctaa gtatttttat tattttggcg ggacttagta gcaatcaagg agcagagtta
      841 catcaagttt taaaaattat tgaaggcgga attcttactg caggtataat actaatttct
      901 ttaagtgctt tagacttgat aaaaaatctt ttatctttat ttaccccttt agtatctggg
      961 ctttacctta tattattacc acttcaatta agcggcacat ttattaaagg aatgctggga
     1021 ataaataatc aaacactgga aattgatcct gtagttgcta ttatatcagt aagtgtattt
     1081 gttttagtac ttttattatc attcaaagca aagggctttc taaaaagtta tgctgtattg
     1141 attggtattt tattaggttg gattctattt ttgatttttg gaaaagctct tattataaat
     1201 cctaaaaata ctgtctttgc cttacctagc gtttttgcct ggggtttacc ggattggaat
     1261 tcaggcatta ttttatcaac agtaataatc acattaatcc ttgtatctaa tgcggtagct
     1321 accatgactg ccattggtga atcaataaaa aagaaaacag atagtaatac acataaacgg
     1381 ggaattttta tgggaggaat tactcatata ctttcttcac ttttttctac agcaggtggt
     1441 ttagtaccat tgcctataac tgctggtttt ataagtctat ccggtcatac acaaataatg
     1501 cctttaatta ttgcctgcat atttttggca gtgatttcct tttttccggt tctaattgga
     1561 tatctagctt tattacctgg tccagttgct tatgcggtat cttttgcatc ttttatacaa
     1621 attattagca tcggattcca cacaatattt aaaaataatt tacaacaaaa acatttcacc
     1681 attctcggta tttccttaat gtttggtatt ggtataatgt ttataccctc tactgtaatt
     1741 aataaacttc cgctattagt acaatatatt ggtagtaatg gactcataac aggtacaatg
     1801 ctatgtattt tacttgaaca attaacataa taatatcttg aaaatctaac tttttaatta
     1861 aaaatataaa tacttcatgt tgctatctga agcaaaacca cgagttatta ctgagattac
     1921 aaaatcctaa atggtcaatt tactgatttt ttacagatct caataccacc aatatctggc
     1981 aacataaaat ctaagagaat aatgtcgttg taaaattata tgagatacca agaaatggaa
     2041 aaccaactat aattaaatga tgatctatac tttgccataa tttataagca ggtattagac
     2101 tttgccctcc caataggaca taatatgtta ttaaaaatgt tactatactg gatggaaatc
     2161 aaataaagta gttgaaaata ttttttaagt ttagaaactt atatgctttc tttatttttt
     2221 cttttccctg gctggttaaa attaaattat cattatcttt ttcaaccaat ttttcttcaa
     2281 ttaaggaatc aagtaatatt tttatttgag gcacctcata tttagcttgc tgcatcccga
     2341 tcccctctag tgtaatatta tcggtaaaat caagttcttc tttaatctct tgtacccaac
     2401 tattatagct ttttctttct ttttcaatct ttattaaaca atattttttt aaacgctctt
     2461 cataaaggtt tttcccaatt ttaaacatac catatttgct atcctcacta tcttggtcta
     2521 atatttcaag tactaggtca aaagcccctt ttaagataag accggctact acaactccta
     2581 ctaatatatc aatgattgga aagttagcta aagatgcaag cataccaact actacactgc
     2641 cggctacaat taaatggttg cgtgaatcta ccgactgtgt cactaaagaa aatctacccg
     2701 ttttaatgcc tacataacgc tggtataaca ttaaaaacaa actaatcagt cctgatagta
     2761 ttacagcagt aaatgttaac cagtcaatat tattctctat cggtacaaaa aaacgactaa
     2821 ccgcctggta tagtgtataa aaaccggtaa taaacataat agaaatcaaa atgtaattta
     2881 caatatcttc aaagtcatat tttattccaa agtatactaa tacactggaa agtccatcta
     2941 agagggtatc taccccatca tttaaaagtc caatactgcc cgataagatg ccaataggaa
     3001 gttttaaaag agccagcaca aaatggacaa tgacagtcac ttttgaaact gccgtaggat
     3061 ttaataaata atcatttatc cactctcggc ttctttttat ttctgaatat gctttctccg
     3121 cttctttttt gccaacttca gtcaggcagt accttccttc atttttttta gcccaaccct
     3181 tatctaataa ttcttctaga gtacttttaa aatagctttc actataatct gtttcattaa
     3241 caaaaaaacc aaatcttttt ttaactttat tgaaatgctc aataatttcc aaaaaagttt
     3301 tcgccccatc cagcattgct actgccactg cccacttctg accatggtca gtatgtctct
     3361 ttttttctaa cataaaatca ccacctaaaa tttaattaat tctttcacaa ctgattttaa
     3421 aaaaatcaac ctttttttaa ttgttcatta aaataatttt tgatcttttc taatgaattt
     3481 aaaaattgtt tgcgttcttc tttagaatcg agtgccccta aaatattatt aaataactct
     3541 ttttctgccc ttatatgcct tttttgagct tttttccctt tttcagtcag ttcaagtcca
     3601 aaagagcgtc tatctctttt gctgattatc cttttaatta attctctctt ttcgagtcga
     3661 tctaccgcac ttgtcaatgt actgcccggt atatcaagta tttcttttat ttcctttaac
     3721 ataatctcgg gattgttatc aacaatattt aaaatactaa cttcaatggt tgtagcatct
     3781 ttaagttctt ctccccaaaa accagtcttt ttattttgaa aattaaaaat gaaataatgc
     3841 caaattttat ctaacttact tattaaatct ttatctatca tgttgttcac aataacactc
     3901 cttttttata cgatattcgt attatattat attagtatta tatttcaaaa tattacttta
     3961 agcaagtaaa aatcttggat ttaataaatt cttaataatt tcatgtttaa aacaaactta
     4021 cattatgata aaattttctc aagctatctt ggaaaataat agacaaatag gagagataat
     4081 ttatgccgca aacagtctct gatgacaaat ataaagtatg gaccagacag cataaaaatg
     4141 ttttgaatga actcaaaaac aagggtgttt ataaagtcaa ggaaagatat ataagaaaaa
     4201 agcttgatac ctgtgcagat atttatcttg atgtgtataa atggcttaga aatcaagtat
     4261 ctaaaagaat ggacatacct aaaaaagcca aatatccaat ctggctcact gtagaagaag
     4321 atcttaaatt accgatggca gatggctatg tattttttga attagaaatt ccaaaagata
     4381 aaataatggt ctttgatatg gctaaatggg actatatagt caactactta tatcttccca
     4441 aaaatgatga agatagaaaa aggttcaagg aaaaattaga taagtataac atcagtgtgg
     4501 aatctgatat ttatttagaa gacttctatc ccatgcttaa aagagaaatg acatccagct
     4561 gggaaaggct atttgatgaa agcattcaac tatcagatca aaaagtagga atcagctggg
     4621 aactaaagga agaatgggtg atcgattatg aaaaaagaga ttgatctatg gactcaacaa
     4681 agcagtatag ttaaagaaaa gttgttaaaa gaagaacgga ccactgttaa aaaacgttat
     4741 attaaaagaa aatataagtc agaggccgaa atatttttaa ctgcatataa tttctttgta
     4801 aaagaagcta aaaaaataat caataaaccc gaagatgctg agtacccgat atgggcagct
     4861 gccgatcgta aaaatgcctt aagtggaggc agcggttatt tgattaaact aaaggtcccc
     4921 cgcgaaaagg taatactttt tgatgcagac aaatggaata aaatattaaa tctgaaatat
     4981 ctacccgata atgaaaagga cctaaaagat cataaaaaaa tgttagatat gtatggaata
     5041 gactctgatt cagatattat actctctcct cactatccaa aattaaaaga aaaaattaaa
     5101 aatagctggc aaaaactatt cgaagacgat atatctaata ttgaaaatat caaagcgggt
     5161 ctctgggaat tgcgtttgga atgggttcaa gacatcagtg aattttaagg gtaattcgat
     5221 atctattctt ctattaggaa aatatttata acactacaaa aggtcccctt aagcttaact
     5281 cgaggggacc tttctgttta atacatatta tttatgatat ggttcattat tcaatatttt
     5341 aaaagcccta tataactgtt ctaacaatat taacctactc atttgatgag taaaggttaa
     5401 atatgataat gacattttat aatctgcttt ttttaaaacc tcttcactca aaccgtgtga
     5461 accaccaatt agaaaggtta tcttactttc gcctctcacc tggaggttct taatagattt
     5521 agctagacct tctgaagtta caggtttacc ttttctatcc agagcgatta tataattttt
     5581 accttccggt actttcgcga ggattctttg cccttcatct tctttaattt tttcaatctc
     5641 agcgtcattc tttttatgtt tactccgggc tggatctatt tcaatcacat caagattgca
     5701 gtaatgttta attctttcaa cataatcctc caaaccatcc agtaaatact cttctttcat
     5761 cttacctact gcaattaaaa ttatattcat ctttatccca cctaaaatta tttaatttta
     5821 tatattttgg ttggtttgtg ttgataagtt aaatctaatt tcagatcttt gtctacaacc
     5881 aggtcttttt tcttcaaaat attttcaaca gttaaatatg caacctttgg gttattattt
     5941 tcctgactaa ggtgacctaa aagaacttgt ggacatctac cattgattaa ctgtggtaat
     6001 atttcagcag ctgcatcatt ggataaatgg cccttttctc ccctgatcct tctttttaaa
     6061 aaacggggat aacttcctga cattaacatg tcaatatcat ggttcgattc taacaataaa
     6121 agatcgagat cggttaatcc ttccataagc tcatcattga tatagcccat atcggtagca
     6181 aataccatct ttttatcatt tctgtaaact acataagcca ccggtgatct ggcatcatgt
     6241 gagattccaa tcggcctgat atcaaaatta ccaaagctaa aatctccttt aaaactttta
     6301 atatgcttac tttttaactt ccctaattca ttttcacagg cggcccaggt accttcattt
     6361 gcatagatag gtatatcaag ccgtcgtgat agaattccta cccctttgat atgatctttg
     6421 tgctcatggg taacaaatat accatctaaa tcatccggat cggcatctat atctttcatc
     6481 ctatttatta tttttttacc acttaaccca gcatctatta gaaaactgtg tttcccgtca
     6541 tcaatataaa ttgaattgcc ggaactacca ctggataaaa ctgaaacttt aactaacacg
     6601 ggatcacctc aattacaaaa ttatacaaat tggttatcaa aatataaaag ggtagagcaa
     6661 tatttattaa gtatcacaac ttataaatag actccacccg ttagctacta attaatataa
     6721 attatgttcg ctcaaattat tcttcgtttt ccatctgcca gcttaaatga taaattaaac
     6781 tgacaatacc taccgccaaa tcctcattcc tgacaaagac atcatcaggt acataaagac
     6841 gggtcggtga aaaattccag atggctttaa taccgtaatc aatgagatta tctactacct
     6901 cttgagctgg atctgcaggt acagttacta ttgccaagtt aacgtctttt tctttaatta
     6961 tttcttctaa tttgttaaca ctttttactt taatcggtcc taccctatta ccaatcttat
     7021 taagatcatt atcaaaaata tcagtaatct caaggcccat tttgccaaag cctgcatagt
     7081 tgattaaggc tcttcccagg ttaccggctc ctattaagac ggccggccat tttttatcta
     7141 cacccaagat tttacctatc gatcttttta gttcttttac attatatcct acaccacggc
     7201 ggccgaattc accataatag gataggtctt ttcgaacctg agttgaaggt atgcctaatt
     7261 tgcctcccag atctttagaa gaaactacat cagtttcttc aaaggtggcg agtttatcta
     7321 aacagcgata atataagggc agtctttcta tcgtagctgc cggtattgaa tctcgatctt
     7381 tcatgttttt accccctgta aaatattatt ttactttact tccattatca attgcttttt
     7441 ctggagtaat taatgtaagt tcgtcttcat tagaggctgc taaaatcatt ccatttgatt
     7501 tcacgccaaa tatttcagcc ggttcaagat tagcaactat tacaatctgt ttaccaacta
     7561 aatcttctgc ttcataatgt tttgcaattc ctgcaactaa ttgtctattt tcttctccta
     7621 aactaacctg gagtttaact aacttattac tatcttcaat tctttctgct tcaattattt
     7681 ttgcaactct taagtccaat ttctgaaaac tttcaaaatc aattaactct tcctcattat
     7741 tacttttctc gttagtcata gttttttcct gctccttttt ttgtttttta tcaaaatatt
     7801 cttccatatc aattctagga tagatcggtt ctgcctttct aacctttatg ttcgacttta
     7861 actgacccca cttaaggtca gaccagtctg catcgattat ctgctggaca tcgccaattt
     7921 gactgccaat ttcataaggg gtttccacca tgaaaggctt aagcattacg gcaatcattc
     7981 tcagtgaatc caataggtta tataatacgg tgcctaatct ctcttcatga ccttcttccc
     8041 ggcctaaaat ccagggctgg gtctggtcaa tatatttatt agttcttctc acaaagttcc
     8101 agagttcctc taaagcatta gtatatctta agtcttccat atgttcttca acttcatcca
     8161 ctgtatttaa acctgtttcc atcagatctt catctatttc atttagatca gattgttcgg
     8221 gtatttcacc atcaaagtac tgattcacca tgctaacagt cctatttaat aagttaccga
     8281 ggtcattggc taaatcagaa tttattctgc gaattaaggc ctcggtcgag tatgtcccat
     8341 cagtaccaaa gggaacctct cttaataagt agtatctgat tgggtcaacc ccaaagtcat
     8401 caataagttc catcggatct actacattgc cccttgattt ggacatcttg ccggactcac
     8461 tcaataacca accgtgtccg aagacctgct tgggtaaggg taagtctaat gccatcaaga
     8521 tgatcggcca aataatggta tgaaaacgta aaatatcttt gccgacaata tgaaggtcgg
     8581 ccggccagta tttattgaag ttctcctcat ctgttagata accaatagcg gtaatataat
     8641 tacttaatgc atctatccag acataaatta catgatcatc atcgatgggt accggtactc
     8701 cccaatcaaa ggtggtgcgg gaaacactta gatcatctaa accctgttcg ataaagttaa
     8761 tcatctcatt acggcgggat tccggctgga taaattcagg gtgatcttta atatggttta
     8821 atagtttatc agcatattta ctcatcttaa agaagtagct ttcttcttca acccattcta
     8881 agggacggcc gcattccgga cagacctttt cttccccgac ctgtctttct gtccagaaag
     8941 tttcatcggg ggtacagtac cagccttcat aatggccttt atatatatca cctttatcat
     9001 ataatttttg gaatatcttt tgtactacct tttcatgtct ttcttcagta gtacggataa
     9061 aatagtcata ctcaacacct aaagcatccc agagttcttt aaaagtctgt actatttcat
     9121 ccacatattc tttcggttct ttatcggctt cttttgcact ccgctcaatc ttctgtccat
     9181 gttcatctga accagttaaa aacatggcat catatccctg cattttttta aacctggtga
     9241 tgctgtcagc tgctacagtt gtataggcat gacctatatg caggcgatca ctcggataat
     9301 aaattggagt tgttacataa aaagtcttgt ctttatccat ggtatcatcc tttcctcatt
     9361 atctatttac atttatttga attgcttctt tatatacttt ctttttttga attcctctta
     9421 cttcggccac ttttttaata gcttcttttt tagtgtaacc gttatccata aataactgga
     9481 catgttctaa gatgctaata tctgtccagc tttctgtatc tacttcctcc tttgttctac
     9541 cttcaattac tacaactatt tcgcctttta tctcagatga tgcaatctta tcttgaattt
     9601 cactgacagt accatatatc ttttcctcat gtatcttagt aatctctcta attaaagctg
     9661 cttttcgttc aggaattttt tcacttaggt cttcaaaaat ttttttgatc cggtaaggag
     9721 attcataaaa gattgtcgtc ttttcttcat tgataatttc ttcaatgaat tgctctcttt
     9781 ctttaccttt acgaggtaca aaaccaaaaa atgaaaattt atcggtttga atacccgaaa
     9841 ccacgagggc gggaattaaa gcggtcggtc ccggtactgg tatgatttca atctttgcct
     9901 gaatggcttc tttaattaaa attaagcccg gatctgaaat gccgggcatg ccggcatctg
     9961 atactaatgc aatatcataa ccgtccttta gttccgttat tagtttttca gctttttcac
    10021 gttcattatg ttcgtggtaa ctggttaagt tattatcaat tttaaagtgg tttaacagtt
    10081 tttccgttct tcttgtatct tccgctgcaa ttaggtcaac tttttctaaa gttttaactg
    10141 ccctaaaggt agtgtcatct aaattaccaa tcggggttcc acatatatat agttttcctt
    10201 tttcactcat ctttatctac tccataaacc ttttttacct catcggtata ctgatctgta
    10261 tctcctgcaa attcaacaat gtttttcttt attttaaagc cgggcttacc gtccattacc
    10321 gcttttagaa taaaacggtc cggatcttta tttgccctgg attggataag ccgcatctct
    10381 ttcggctcca ggtttttttc ttttaaaaaa tagaaaattt gaggcagcct tttagtgagg
    10441 tgaactaaat aaaatttgcc gccggctctt aaaagccgga ctgtgttatc aattatttcc
    10501 ttaatatcta tttcaatttc atggcgggca attgccttat atttattttg agtaatttta
    10561 ccggagtcag ttgttctata agggggattg gtcactacag tatcaaactc attatctgta
    10621 aagtgactca ttacctcttt gatatcccct gcaactattt caatcttatc ttctaaacca
    10681 tttaacttta cagaccttct tgacatttcg accaaaggtg actgtaattc tatcccatat
    10741 ataatatcag gattgttttt ataagctaag agcagtggga taacaccgct accactgccc
    10801 aaatcaatga tcttatcatt gtttttgact tccacataat ttgctaagag tacagaatct
    10861 gtaccaaatt taaagaaatc atcattttga attattttta gttcgtccgg tattagataa
    10921 tgtatctctt ccactagact tccccttctg tttattatta tcttctaaca accttaaaca
    10981 aaaaagacat tcgccttttc ttttttcacc aaaacttaaa tgacagatat gaaatccttc
    11041 atcatgcagt ctttttaaat tatttttgct ttggggaagt tgttgttctt cctcttcaaa
    11101 tatgatcgat ttcagttctt cattttcatt tcttaactct tcattctctt tatataattt
    11161 ataagtaata tcctttaaat tcttaaattt catcgaaatt tcttcaatct tttcctggaa
    11221 caaagctagt tcttctaaaa tttcttcgtc cataagccat cgctcctaat tcttttcttt
    11281 aatctctttg atttcttcta ggtcaaattt tattacatct ccgtctttat ttttcaactt
    11341 tacctgctgt ttgattaaat tccggtcaac aacttccgcc ttactaccat tagtcagggt
    11401 tattttttct cccacttccg gcatcttttc ggtaatctct tcataataat cactttcata
    11461 ttttaaacaa cacattaatc ttccacataa acccgagatc ttagcaggat ttaaagctaa
    11521 ttcctgatct ttcgccaact gaatagagat cgggtcaaaa tctctcaacc aggtatcaca
    11581 acaggtacgt ctaccacagg gtcctaaacc accaatcatc ttggcttcat ctcttacccc
    11641 aatctgtcta agttcaatcc tggttttaaa tacacgggcc aaatctttta ctagctctct
    11701 gaaatcaact cttccatcag ctgtaaagta aaatatgact ttattatggt caaaagtata
    11761 ctccacatcg atcaacttca tcggcagccc gtgttctttg atcttatcta aggctatatc
    11821 ataagcttca tcttctagtc tcttattttc ctcatcttta tctatatctt ttaaagttgc
    11881 tttccttatt acctttttca aaggtgctac aagttctttt tctgcaactt tctttttctc
    11941 taacacaact tctcccatct caattccacg ggaggtttcc actatcactt tatctccctt
    12001 ttctacttct atattatcag gatcaaaata atagattttg cctgctctct taaaacttac
    12061 tcctattact tcttccataa attataactc ctttttattt cttatcttaa ttaataaaga
    12121 aggaaaaagc agttccttct ttatatttct gtaataataa tctcgataca tatttattaa
    12181 ttttgttagt tcaaaaagtt gttcggttga ataattttcg gtcgctttag ttattaaatt
    12241 tttaaagtca aagttaatta tatcattatt tccgtttctt ttcatcacaa gaatatctct
    12301 aaaaaagctt agaatcagtt caaataaagg aaattccgta tcacttaact taattaattc
    12361 atcggctgaa ttatatattt caaaattatt tttgctttcg atacctgcaa taaattcaat
    12421 aattctattt cggttttcta caaagtcttc tttgccggcc aatttaacag cctttttata
    12481 cttacccctc gccaaggtgg cataaaggtg ggcatcttcc ttattaaatc cttctctatt
    12541 tattagataa tcttctacta tatcagttgt ttggttatgt aacttaatct cctggcacct
    12601 ggaaattatg gtcggaatca attcgttctt attttcattg gtcaaaatta ttatcgcgta
    12661 ttcgggtggt tcttctaatg tttttaataa actattctgt gcttgaatag tcattctttc
    12721 ggcctgatca atgatataaa ccttatgatc ggattcatag ggtttataac ttatctcttt
    12781 ttgcaaatct cttatctgat cgattaaaat atttttgtgg tcttcttcta ccataatttt
    12841 atgaaaatcg gggtggttac catgttcaat cttcttacag cttaggcatt ccccacagcc
    12901 gtctgcaata ttattttcac aaaaaatagc ttgggcaaat tctccagcca tctcggattt
    12961 gcccaaacca ttattacccg tgaatagata agcatgacta attcgatttt ttttaataga
    13021 atcctttaat attttttttg ttttggattg tccgggaata ttattaaaac tcattaaaat
    13081 ctcctatcat cgcttacatt ttgataaact gttctacatc catgataaat acagtcgctc
    13141 ccccaatttt aacatccatg gggaaagaat agtaactttc catagagtta gcaactggag
    13201 acatcggagc aattgtcttt ttacgggcct gacaattttc tttaataatc tctaaaactt
    13261 gatcaacatg ttcttcatca gtcccgatca tgaaggtggt attaccttct cttagaaaac
    13321 cgccagtagt tgccatcttt gttgctttaa agtttgagct actcagagcc tccatcaaat
    13381 catgagcatc atgatcatga acaacagcga ttaccatttt aacggttttg ctttcactca
    13441 taataactcc tcctttattg cctttaaaac atcctgaaaa atctcttcag gtgctctact
    13501 ggcgtctatt actttaaatc tatcctcttt ttcagccatt tctaaatagg cttctctgac
    13561 tctttcatga aattcaatca attctctttc caatcggtcc cccgacttat caggagttag
    13621 atctctcgcc ctttgtaaac cttctcttac atccaggtca agcaaaattg ttaaatccgg
    13681 ccagtaatct ctaatgaccc attcgttaat ttctcttacc attttcatat caaggcctcg
    13741 gccgtgtcct tgataggcaa tattggaatc tatatatcta tctgaaataa ctatctttcc
    13801 ttcttcgatt gctgggatta tggtttctac cacgtgctgc gctctatcag cagcataaag
    13861 aagtatttct gctctatcat ccatcttatg gtgtatagga tccaataaaa tttttctgat
    13921 cttttcccct atcttggtac ccccgggttc cttagtaaat actacttcat aatctaaatc
    13981 tcttaaatat ttggttaata gtttcatctg tgtagatttg cccgaaccct cgataccttc
    14041 aaatgtgata aaaaaacctg acatcctaga atgtctcctc tcctaaacaa ttagccaaca
    14101 tcttacattt ctgaactaat aatatcatat taataggggt atttcaatta agtgtgaacc
    14161 gatattaaaa ataagaaaag accagcaaag aggttatgct ggtcgtgaaa ttaaaattta
    14221 ttttattttt aataaccgag taaatattta tctacctgca tggctgcctt agctccggta
    14281 ccagctgcta caactacctg attgaagttg ggttcctgaa catcaccggc tgcaaagatt
    14341 ccttctttat tggtcctttg tttattatca gttattatat agttcttttc atcaagctct
    14401 atttgaccct taaagagttc agtcctcgga tcataaccta cagccataaa taagccttta
    14461 atgtctaagt tttcacgttc cttggtttta ttattttcaa ttattatacc atctaaatgt
    14521 ttatcacctt ttaactcatc gacctctcta ttccagagga tttcaacctt ttcattctct
    14581 tttattctgt cagctaaaat cttatcgcct cttagtcgct ctcttctgtg aatgagatat
    14641 actttagaag caaattttgt taagaaatca gcttcctcta atgctacatt gccaccgcca
    14701 acaacggcaa cctctttacc tttaaagaag gcaccatcac aggtagcaca gtaagatata
    14761 ccattaccag ttagttcttc ttctctatat aatcctaatc ttttagcttc tgaaccagtt
    14821 gctattatga cagattctgc ttcatattta gtagaatctg tttcaataat ataaggtttt
    14881 tcttcaaatt gtaccttttc aactatctca aaaaccattc tcgttccaaa cttctcggcc
    14941 tgttcagtaa ccttttccat gatttcaaat ccaccgactc catccgggaa accgggatag
    15001 ttttctaaat caggggttaa agtaatctgg ccgcccggtt ctggaccatt aataactaat
    15061 gggtttaaac cggccctaga agtataaata gctgaagtaa gtcctgctgg tcctccacct
    15121 atgatgatca gtttttcctt ttgaatatta tcagacatta tatcatctcc tcttaaataa
    15181 acattccaca atatattcta atatgaaaga aagatgattg ctgttaaatt cttaacaatt
    15241 tttagattag tcaatactgt aattaggtgc ttcttttgta atatcaacat catgggggtg
    15301 actttcgcgt agaccagccg aggtaattct aacaaattca gcatcttttt gtaaggcagg
    15361 tatatcggct gtaccgcagt aacccattcc agaacggaga ccgcccacta attgatagat
    15421 tgtgtcagac agtttccctt tatagggtac ccttccttca ataccttccg gcacaaactt
    15481 ctcttctttt ttgccttcct ggaagtatct atccttgctg ccttttttca tagcgtcaac
    15541 tgaacccatg cccctatata ctttatagct tctaccttta tatatctcaa gttcgccggg
    15601 actttcttct gttccggcta atagactacc gatcatgacc gtgtttgctc cggcagctag
    15661 tgctttaact atatcaccag aatatttgat accgccatct gcaataattg ttttaccata
    15721 ttttttagcc atctgagcgg cttcattaat agctgtaacc tgaggtacac ctactccagc
    15781 taccacgcgg gttgtacaga tcgagccggg acctacccca acttttataa cgtccgcacc
    15841 ggctttaatc agttcttctg tggcttccgg tgtagcaaca ttaccagcga ttaaagtaat
    15901 ctcagggaac ttcttcttga tatccttaac catgtcatta accttggtgg aatgtccgtg
    15961 ggccgtatcg actaccacaa catcggctcc ggcttcaaaa atcgccctta ccctgttaaa
    16021 ggtgtcttta ccggttccta cggcagcacc taccaacagc tgaccgttat cgtctttggc
    16081 cgaatttgga tacttgcggg ccttctcaat atccttgata gtaatgagac ccttgagtat
    16141 aaagttctca tcaacgagtg gtaatttttc aatcttatgt ttctgtaaaa cagactttgc
    16201 ttcttcaact gtagttccca ctggggcagt tactaaacct tctttggtca tgacttcttt
    16261 tatcggtcta tcaaagtcct gttcaaatcg caaatcccta ttagttaaaa taccgattaa
    16321 tttgtagttg ttatctacaa tcggcacacc agagatatgg tactttgaca tcagttcttc
    16381 cgcttcataa atcatatcat caggtccaag tgagaatgga tcaacgatta caccactttc
    16441 agatctcttt accttgtcta cttcatcagc ctgttctcta atcgacatgt tcttgtgaat
    16501 aatccccaag ccaccctggc gggctattgc aattgccagc ctggattcag ttactgtatc
    16561 catcgcggca ctaataatag ggatattcaa ggaaatatct tcagataact ctgtactaac
    16621 atccacttct ttgggcaaaa tatccgactt tgccggttgt aataatacgt catcaaatgt
    16681 taaagctact ttttcaaatt tcttatccat tttttaactc acctcgcttt ttttggaaaa
    16741 aatatattat ataaaaataa accccaggat aagatcctaa gggtttttat aatactaata
    16801 tttaatttct cttcttcctt ccaatgcttt agacagggtt atttcgtcgg caaattctaa
    16861 atctcctccg accggtaaac cgtgcgcaat tctagtaacc tctacatcga gaggttttaa
    16921 taattttgcg atatacattg ctgtcgcatc tccttcagca ttggggtcag ttgcaactat
    16981 tacttcttta acgccctctt ccaggcgagg aagcaggttt tttatctcta tttcatcggg
    17041 gcctatcccg tccatgggtg aaattgcccc atgcaaaaca tggtaaagcc cattatattc
    17101 acctgttctt tccattgcga taacatcttt aggactttca actacacaga ttgtctcctg
    17161 atccctttcc tctgaagtac agtattcaca ggggtcttgt tctgtcaagt tattacatat
    17221 agaacaatat ttggtttttt ctctggcatc aataattgca tcggccagat ccttaacttc
    17281 ttgtttagaa cgacctagaa gataaaatga aagtctgcgg gcagtttttt gacctattcc
    17341 gggcagtttg cttaattcac ctattaatct tcccacaggt cccggatagt atttcattta
    17401 aaacataccc ggcaagttca tgccaccggt catttgaccc attttttcat ttaattcttc
    17461 ggtcgcttca ctcatggctt tattaatcgc tgccaggatt aggtcttcta acatttcaac
    17521 atcttctgga tccacgacat cttcgtcaat ctgtagatcc aatacttctt ttttaccatt
    17581 aactaaggct tttacagctc cgccgccgga tgtagcctca taagtcttat tttcaagttc
    17641 ttcctgcatt tctgacattt tagcctgcat tttctgagcc tgtttcataa gtttgttcat
    17701 attcatcaaa attacctcct taaggttaaa tttttattta tttgaaatta tgtcggggtt
    17761 tgtttcaatt atttcaccat taaacatctt agccacctgc tctacactga tatcccccga
    17821 gtttttattc atactatctt ctgcactttt attttgatta tcattttgtt ctttttcctg
    17881 attattatta tccacttcaa tattatcttc tgtggaaaca atctttataa taaggtttct
    17941 tgaaaagaga ctggataaaa atctgccaat gtattccttt ttttctttag ccctcttccg
    18001 atggaagttt ttatcaggag gaaaactaat ataaaccttg tcattaacta ctttaatagg
    18061 tttcccctct actaaaacag catgtaaact aatatcttct tttttaatat tacccagtag
    18121 ctgcggccag tattttttaa cctcgtgtag atctaaaccc tcttttgaga cttgtttttc
    18181 ctctttgctt tctttagctg ctttttcttc ttgttcggtc tccacagttt gttttacata
    18241 atcctgttct tctggtgaag gaacttcatc gtcttctttc tcactctctg tctctcggtc
    18301 cgtctcactt tgcacagctg cttgagcagt aatatttcct tctgtaattc tattttctaa
    18361 atacttaact ttttctttta attcttttac ttcctgggtt aaatccgctt cctgcagttt
    18421 gtctgacatt ttaaaaatac caacttctag attgatgcgc gaatttccgc ctaaagaaat
    18481 ctcatcttta atctcattta acacttcaac aattttagtt aaatgactga gagaaaattt
    18541 ctctgctaat ttttccattt cacttaggcg ttccttgggt aattttacta aatttgtctt
    18601 tataccgcat tcctttatta aaaggagttg attacagtat tcctttaaat cttcgatcag
    18661 tctatctaca tctttacctt cattaagtat ttcattaaca agtgagatgg ctgcttgagt
    18721 gtttttctct ttcaaatcca tcattaattc tctgagttct tggccggaag ttctacccaa
    18781 catctcttca ataatttcgg tttttatatt ctcgcccgtg tatgaaaccg cctgatctaa
    18841 gatacttaag gcatctctta aaccgccctg agaactcctt gcaattaaat taagggcagc
    18901 tacctcatag tctaaatctt cttcttgaca tatatattct aaatggtttt caatttcttt
    18961 ttctgatatt aatgagaagt caaatctttg acatctcgac atgatagtgg gtattacttt
    19021 atgtggttca gttgtagcta atacaaaaat tacattttcg ggtggttctt ctaatgtttt
    19081 aagcaaagca ttaaaagcac cggtagttaa catatgtact tcatctatta tatatacctt
    19141 atacttgcct tcaccgggat aaaatttaac tttctccctc agttccctga tctcatcaat
    19201 gccacggttg gaggcggcat caatttcaat tacatcgatc gaattaccgg atttaattct
    19261 tttacaggaa tcacataccc cacagggttc tgtcgtggac tcctcctgac agttaaccgc
    19321 catagctaat actttggcag ttgatgtttt accggttccc cggggacccg caaatagata
    19381 ggcgtgtgaa atccgatcat tttcaattgc attaagtagg gtctgtgtaa cggctggttg
    19441 tcctattatt tcattaaatt tttgtggtct atattttcta tatagggata aagaaggcat
    19501 aactatttat caccacttaa ttttataaga ttataaaaaa ttggccgtac accggcctcg
    19561 aattttcttt cccaggcgtt accccagcag ttagttcaaa ccaggcgacc tcgcggcaca
    19621 tggaggcatt tacttaccgc tgcttcctcc cggacctgac ggggttcgta aagctccatt
    19681 gcgcgagacc cgatcatcat caccacttac cagggccaga ccctgaaaaa gaaaaccctc
    19741 agctcagctt caatcctgct atagcgggtt gcaggttaca gggtaccgct acccccccat
    19801 ttagtacggc caaatgtttt gtcaaaattt tagtataaaa tggcggagag ggagggattc
    19861 gaaccctcgg taagcaacaa ggcctacaca tgatctccaa tcatgctcct taagccagac
    19921 tcggacacct ctccatgct
//
LOCUS       DATGCJ010000267         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_525895, whole genome shotgun sequence.
ACCESSION   DATGCJ010000267 DATGCJ010000000
VERSION     DATGCJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525895"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VKN64_11870"
     CDS             <1..195
                     /locus_tag="VKN64_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60973.1"
                     /translation="VMENRTVILISHRISTIKNADIIFVLDEGEILERGTHEELIANE
                     KLYFDIYQKQQLEEKIANIN"
     gene            213..2279
                     /locus_tag="VKN64_11875"
     CDS             213..2279
                     /locus_tag="VKN64_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMA60974.1"
                     /translation="MNQDFREEKIMGKAYDSRLMKRLLEYAYPYWKILLVCIFLLLLV
                     TGVDLARPYLIKIAIDDHINALDKPMVTFEVDQYENKGTVYNGQRYIRVGNIESTFPD
                     KQRFQLFEIENEYYLIEGIINEKEEIEVIDGNQFTEIRNGNNIYNARAINEDVIKEFR
                     AQDYNALTRIGLIFLSMIVFGFGVNYLQIYLLNKTTQKIIYNMRQEIFSHLQKMSLSY
                     FDKNPVGRLVTRVTNDTETLNEMYTNVLVNLFRDIFIIIGIVIIMFRLNFTLSLISFT
                     IVPVVIIAAFIFRSYARDAYRMVRIKLAKINSALSENISGMKIVQIFNQEKRKLKEFI
                     NINDEYFDASMRQIKIFAIFRPSMDLMYSFALALLIWFGGARVYNEVLQFGVLYAFIN
                     YIQKFFRPINDLSEKYNMLQSAMASSERIFKILDTEVDIKEPEEPVALSENINGKVEF
                     KDVWFAYEKEDWVLKNINFTIKPGQTVALVGATGAGKTSIINLISRFYDIQNGEILID
                     DTNIKDIKKSELREKIGVVLQDVFLFTGDIGSNISLNADIKKEKIKETAEYVNADKFI
                     SKLSNKYEHEVKERGSTLSAGQKQLLAFARALAFDPDILVLDEATANIDTETEMLIQD
                     ALEKLTADRTTIVIAHRLSTIQHADKILVLHKGRIKERGTHQQLLAEEGLYYNLYQLQ
                     YKDQDI"
     gene            complement(2690..>3088)
                     /locus_tag="VKN64_11880"
     CDS             complement(2690..>3088)
                     /locus_tag="VKN64_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60975.1"
                     /translation="FRDEFFDTREDLVEELQDKRYELREAIITDESEEVISELESEVS
                     DLQNQVTSARTEYLKKMKSVLTEEQINMMIENEYRIGMGSEFGFRSPVTSFNRMPMNG
                     NRFHQFQGRTFRSFRGMGGNFRTPARGYCY"
BASE COUNT         1071 a          505 c          530 g          982 t
ORIGIN      
        1 gtaatggaaa ataggactgt tatcttaata tctcacagaa tatcaaccat taaaaatgcc
       61 gatattatct ttgtattgga tgaaggcgaa atactggagc gaggtacgca tgaggaatta
      121 attgccaatg aaaaattata ctttgatatt tatcaaaaac aacagctgga agaaaaaatt
      181 gccaatatta attgatttaa ggatgatcaa ctatgaatca ggattttaga gaagaaaaaa
      241 taatgggaaa ggcctatgac tcccgtttaa tgaaaagact attagaatat gcctatcctt
      301 attggaagat actcttagtc tgcatatttt tattactgct cgtcaccggt gtagatctgg
      361 cccgccccta tttaataaag atagctattg atgatcacat caatgccctc gataaaccga
      421 tggtaacctt tgaagttgat caatatgaaa acaaggggac tgtctataat ggtcaaaggt
      481 atattagagt gggaaatatc gaatcaactt ttccggacaa acagagattt cagctgtttg
      541 aaatagaaaa tgaatactac ttaattgaag gtattatcaa tgaaaaagaa gaaatcgaag
      601 taatcgatgg caatcaattt actgaaataa gaaacggtaa taatatttat aatgcccggg
      661 caattaatga agacgttata aaagaattta gagcccagga ttataatgcc ttaacaagaa
      721 ttggcttgat ctttttatct atgatagttt tcggctttgg agttaactac ttacagatct
      781 atcttttaaa taaaacgacg caaaagataa tctataacat gagacaggag atcttctctc
      841 atttacaaaa aatgtcgctg agttactttg ataaaaatcc ggttggtcgt ttggtaaccc
      901 gggttaccaa tgatactgaa acccttaatg agatgtatac aaatgtttta gtaaacctat
      961 ttagagatat ttttataata ataggtatag taataattat gttcagattg aacttcacct
     1021 taagcctgat ttcttttacg atcgtaccgg tggtgatcat agccgctttt atttttagaa
     1081 gttatgcccg cgatgcgtat agaatggtga gaattaaact ggctaaaatc aactcggccc
     1141 tttctgaaaa tatctcaggg atgaaaatcg ttcagatttt taaccaggaa aaaagaaaac
     1201 taaaagagtt tattaatata aatgatgaat actttgacgc cagtatgaga cagattaaga
     1261 tatttgccat ctttcgaccc tcgatggact taatgtactc ttttgccctc gccttattga
     1321 tctggttcgg gggagcaagg gtctataatg aagtattaca gtttggtgtc ctttatgcct
     1381 ttattaacta tatccaaaag ttcttcaggc cgatcaatga tttaagtgag aaatacaata
     1441 tgctgcagtc tgccatggct tcctcagaga ggatctttaa gatactcgat acagaagttg
     1501 atatcaaaga accggaagaa ccggttgctt tatcagaaaa tataaatggt aaagtagagt
     1561 ttaaagatgt ctggtttgcc tatgaaaagg aagactgggt cctaaaaaac attaacttta
     1621 caataaagcc cggtcaaacc gtagccttgg taggggcaac aggagctggt aaaacctcta
     1681 ttataaattt aatcagtaga ttttatgata ttcaaaacgg ggaaatatta atagatgata
     1741 ctaatattaa agatattaaa aagagtgagt taagagaaaa aatcggggtc gttcttcagg
     1801 atgtattttt atttaccggt gatatcggca gcaatatttc tttaaatgcc gatattaaaa
     1861 aagaaaaaat taaagaaaca gccgaatatg taaatgccga taaattcatc tctaaattat
     1921 ctaataaata tgaacacgaa gtaaaagaaa ggggttctac tttatcggcc gggcaaaagc
     1981 agctgttagc cttcgccagg gccttagcct ttgatcctga tattttagtt ttagatgaag
     2041 ctaccgccaa tatagatact gaaactgaga tgttaattca agatgcactt gaaaaactaa
     2101 ccgctgacag gacaacgatc gtcatcgctc acaggctctc taccatacag catgcagata
     2161 aaatattagt gcttcataag ggtagaatca aggaaagagg aacccatcaa caactattag
     2221 cagaagaagg gctttattat aatctatacc aactgcagta caaagatcag gatatctaat
     2281 cttgattatt tttataaagt tgcaggaata attatttttt taaagaaata tatctatacc
     2341 aggaggcgat gttatggaat taaatccgcc tgaaaaagat ggttattatg ccctactttt
     2401 agcaatcttg aaaggctact cagccagtaa agccttatct tatattaaaa acggacaatc
     2461 aagagcttaa taatttgtaa aactccatat ctttatatgg agttttcgtg tataaaaatc
     2521 cttataatta ttgatttatt cactacttat ccacaaattg tgtataactg ataacaatta
     2581 ctatttaaaa tttacaaaat aactcaatat ttgttaaata ttagctatat taaggcaatt
     2641 accccgccta aacggggtaa ttttttttat ttgatatatt tactttttct taataacaat
     2701 aacctctggc aggagttctg aaatttcctc ccattccacg aaaacttcta aaagttctcc
     2761 cttggaattg atgaaatcta tttccattca taggcattct attaaaagaa gtaactggtg
     2821 acctaaaacc aaattcactt cccattccaa ttctgtattc attttctatc atcatattaa
     2881 tctgttcttc agttaaaaca cttttcatct tttttagata ttctgtgcgt gctgatgtaa
     2941 cctggttctg taaatcagat acttcacttt caagctcgga tattacctcc tctgattcat
     3001 cggttataat tgcttctctt aactcatatc ttttatcctg aagttcttcc actagatctt
     3061 ctcttgtatc aaagaattca tctctgaa
//
LOCUS       DATGCJ010000268         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_527756, whole genome shotgun sequence.
ACCESSION   DATGCJ010000268 DATGCJ010000000
VERSION     DATGCJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527756"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VKN64_11885"
     CDS             <1..1179
                     /locus_tag="VKN64_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HMA60976.1"
                     /translation="KKQTKMLKEDGVTGEGLPSLGTKVLDNIINSNGLYPTNNFQYGT
                     FEGTEKVAGEALVEKGYLQSNKGCYGCPIRCGRDVELPNGRSGEGPEYETGWAFGADC
                     GVDDLNAITEANFLCNELGLDTISAGTTIAAAMELYEKGYIDKEELENGPELNFGSSE
                     AIVYYTKQMGYREGFGDKLAMGSYRLAESYGHPEIAMTVKKQELPAYDPRGVQGHGLQ
                     YATSNRGGCHVRGYMISPEILGSPEKLDPQELKGKPEWVKIFQDLTAIIDSVGMCLFT
                     SFALGLDDYRDIVNAATDFNYSSEDLLQAGERIYNLERQFNLEAGITPDEDKLPKRFT
                     EEPLPDGPQKGEVSKYKELLPKYYEVRGWTEEGLISKNKIKELGIESSEFKKVDKDIG
                     "
     gene            1418..2725
                     /locus_tag="VKN64_11890"
     CDS             1418..2725
                     /locus_tag="VKN64_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMA60977.1"
                     /translation="MEFSINNPQFWRKFGGEQDQIEELELEIDQDGNIKIPDKIRKKL
                     NLPGGTELKVEVGNNGMKIKRVDPNLTRVYIEPTTDCNLDCEMCLRQAWDENIGNMNY
                     DTYQKLINDLKDFDYVERISFWGIGEPLCHKDIVKMVRAAKKLNIETQIITNGLLLDD
                     DMSKNLLEADLDSLIVSIDGTSPETMSNIRSGAELAKIIKNVKRFNYLNNNKIKANAE
                     IGIEYVIMQSNINELTDLRKLAFELGASFVILTNLLPYTEEMTDEILYSSSISRSKPT
                     NRSPLRPEVYLPPVDLKEDVAKHIAKVMSKTSSLSTRQVPFDPHGGYCNFIEEGSIAV
                     NYRGEVSPCVPLMHSYNCYIRERKKHIEGYYLGNVNEKSINEIWYSDEFKSFRKKVIE
                     FPFSECTQCSGCEFSEQNKQDCHGNEFPVCGDCLWAKGVIQCP"
BASE COUNT         1037 a          349 c          567 g          835 t
ORIGIN      
        1 aaaaaacaaa ctaagatgtt aaaagaagat ggagtaacag gagaagggtt accttcttta
       61 ggtactaaag ttttagataa tattataaat tcaaatggac tttatcctac aaataatttt
      121 cagtatggta cctttgaagg tacggaaaaa gtagccggtg aagcactagt agaaaaaggt
      181 tacttgcagt ccaataaagg ctgttacggc tgcccaatcc gctgtggtag agatgttgaa
      241 ctaccaaatg gaagaagtgg tgaaggacca gaatatgaaa ctggttgggc gtttggagct
      301 gactgtggtg tagatgattt aaatgcaatt acagaagcta actttttatg taatgagcta
      361 ggtctggaca ccatcagtgc aggtactact atagctgcag ccatggaatt atatgaaaaa
      421 ggatatatag ataaagaaga attagaaaat ggaccagagc ttaatttcgg ttcctctgaa
      481 gcgattgtat attatacaaa gcagatgggc tatcgagaag gatttggaga taagttggca
      541 atgggttcct atagattagc agaatcttat ggacatcctg aaatagcaat gacagttaaa
      601 aagcaggaat tacctgctta tgacccgaga ggggtccagg gccatggtct tcaatatgct
      661 acttctaata gaggcggctg ccatgtaagg ggttatatga tttcacctga aatattaggt
      721 tctccagaaa aattagatcc ccaagaatta aaaggaaagc cggaatgggt taaaatattc
      781 caggatctaa ctgctattat tgattctgta ggaatgtgct tatttacatc ttttgcatta
      841 ggattagatg attatagaga tatagttaac gctgctacag attttaatta tagctccgaa
      901 gatttattac aagctggaga aagaatatat aatctggaaa ggcagtttaa tttagaagcc
      961 ggcataacac ctgatgaaga taaacttccc aagagattta cagaagaacc tttacctgat
     1021 ggtccgcaaa aaggtgaagt ttcaaaatat aaagaacttt taccaaaata ttatgaggtg
     1081 cgcggttgga cagaagaagg attgattagt aaaaataaaa ttaaagaatt aggtattgaa
     1141 tcatctgaat ttaaaaaagt tgataaagat ataggataat aaatgtaata aataattatt
     1201 ttttaagagc cgggctggcg atagtaagtc cggctcttaa taacttttaa tattgtttta
     1261 aacatttttt aagctcgttt tatttattaa tattattaat tttttatgtt ataattaatt
     1321 aaatctaatt tatatagagt aagaaaagct ttaacttttt ataaagttgt tattcaaaaa
     1381 cagtaatatc aataataata aaaaagaagt gataataatg gaattttcaa ttaataatcc
     1441 acagttttgg cgcaaatttg gtggagaaca agaccagata gaagaattag aattagagat
     1501 tgaccaggat ggtaatataa aaattcccga taagataagg aaaaaattaa atctacctgg
     1561 tggtactgaa ttgaaagtag aagtaggaaa taatggcatg aaaattaaaa gggttgatcc
     1621 taatctaact agagtttata ttgaaccgac tactgattgt aatttagact gtgaaatgtg
     1681 tcttagacaa gcctgggatg aaaatattgg taatatgaat tatgatactt atcaaaagct
     1741 gatcaatgat ctaaaggatt ttgattatgt agaaagaata agtttttggg gtataggaga
     1801 accattatgc cacaaggata ttgtaaagat ggttagagca gcaaagaagt tgaacattga
     1861 gactcagatc ataactaatg gtttactttt agatgatgat atgtctaaaa accttttaga
     1921 agcagatttg gatagtctta tagtttctat agatggaact tcgccagaaa ctatgtctaa
     1981 tataaggtca ggagcagaac tagcaaaaat cataaaaaat gttaaaaggt ttaattatct
     2041 aaataataat aaaattaagg ctaatgctga aatcggaatt gaatatgtaa ttatgcaatc
     2101 taatataaat gaattaacag atttgaggaa attagcattt gaactgggag catccttcgt
     2161 aatattaaca aatcttttac cttatacaga agaaatgaca gatgaaatat tatattcttc
     2221 cagtataagt cgttccaaac ctactaatag atcacctcta agaccagagg tatatttgcc
     2281 gcctgttgac cttaaagaag atgtggccaa acatatagcg aaggtaatga gtaagacgag
     2341 ttcattaagt accagacagg taccttttga cccgcatggg ggttactgca attttattga
     2401 agaaggcagt atcgcggtaa attatagggg cgaagtcagt ccctgtgtgc cgctcatgca
     2461 cagctataat tgttatataa gggaaagaaa aaaacatatt gaaggctatt atttagggaa
     2521 tgttaatgaa aagtcaatta atgaaatatg gtatagtgat gagtttaaaa gttttagaaa
     2581 aaaagttatt gaatttccat tttcagaatg tactcagtgc tctggctgtg aattttcaga
     2641 acaaaacaaa caagactgcc atggtaatga atttcccgtc tgtggtgatt gtttatgggc
     2701 aaaaggagtc atacaatgtc cctagattat ttgatctgta gttgaaatta aaaactgata
     2761 aagaggaaaa ggttaataat atcattaa
//
LOCUS       DATGCJ010000269        12415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_484349, whole genome shotgun sequence.
ACCESSION   DATGCJ010000269 DATGCJ010000000
VERSION     DATGCJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484349"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..75)
                     /locus_tag="VKN64_11895"
     rRNA            complement(<1..75)
                     /locus_tag="VKN64_11895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(445..2973)
                     /gene="gyrA"
                     /locus_tag="VKN64_11900"
     CDS             complement(445..2973)
                     /gene="gyrA"
                     /locus_tag="VKN64_11900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMA60978.1"
                     /translation="MVDEFGGKNVIQVDIDDQMKDAFLDYAMSVIVSRALPDVRDGLK
                     PVHRRILYALHDLGMTPNKSHKKSARIVGEVLGKYHPHGDTAVYDTMVRMAQDFSYRY
                     PLVDGHGNFGSVDGDSAAAMRYTEARMAKITTELLRDLNKDTVDFIPNFDDTLKEPDV
                     IPSRFPNLLVNGSSGIAVGMSTSIPPHNLREVIDGIVRVIDNPDIDLAELMEVIKGPD
                     FPTGGLIMGKGAIYKAYKTGRGKVTVRAKARIEENETSADRIIIDELPYRVNKARLIE
                     KIADMVRNEKIDGISDIRDESDRRGMRIVIKIKRGSNPKIVLNQLFKHSQMQVTFSII
                     MLSIVEGEPKVLNLREIIDHYLDFQKEIITRRTKYELDKAESRAHILEGYMVALDDID
                     NVIELIREADDVDTARQQLINKYELSEDQANAILRMRLQRLTALERDKIDAEYADLKE
                     DIKYYKEILGSEEKKLEIIKEEIREIRDRYGDERRTKILNQTTDLDLEDLIEEEQVVV
                     TMTNQGYIKRMPVDTYRSQRRGGKGMIGTTTREEDFVENVFITSTHNYFLFFTNKGLV
                     YRLKGYQIPQASRQSRGTAIVNLLQLEGDEKVSSIIPLSDFEESKYLTMVTKKGMIKK
                     TPLVEYDSNYTGIIGIKLQENDELMEVKNTNKGENVILVTHQGKAIRFNEKDIRSVGR
                     AAQGVKGIDLADDDYLVGMGMDSEGSDVLVISEKGYGKRTDVENYRIQQRYGKGIITL
                     NITDKNGPIASTKVVNKEDEIIIITAEGIIIRTRVEEISKTGRNTMGVKVIDVEEDDY
                     VVSVGKIKPEDLEEEADEEVEKEKEENKKVEKEDKEENEEKVDQ"
     gene            complement(2993..4921)
                     /gene="gyrB"
                     /locus_tag="VKN64_11905"
     CDS             complement(2993..4921)
                     /gene="gyrB"
                     /locus_tag="VKN64_11905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMA60979.1"
                     /translation="MAELKKGRYEAENIQVLEGLEAVRKRPGMYIGSTSIDGLLHLVY
                     EVVDNSIDEAMAGYCDRIEVNLKKGNIVTVVDNGRGIPIETHPKVNKPAVEVVMTTLH
                     AGGKFDGEGYKVSGGLHGVGVSVVNALSEWMTVEVKWKDGNIYRQKYRRGVPVYDLKQ
                     IGTCEVERETGTKITFKPDPEIFEEINFRYDKLLQRFKEMAFLNKGIHIKVIDERDEE
                     EVKEEEFHYEGGIVQFVKYLNKNKKPLFPDPVYLEESEENKDLEISFQYHSGFKERIF
                     TFANNINTREGGTHLSGFKSALTRTVNGYARSKGLLKEKDDNLTGEDVREGLTAVINI
                     KIEEPQFEGQTKTKLGNSEVRGFVDSALSKHLKAFMEENPSVAKTIVEKGIKAAKARK
                     AAKKARDLTRRKNALISTSLPGKLADCSSRDTENTELYIVEGDSAGGSAKQGRDRNFQ
                     AILPLRGKILNVEKARLNKILNNNEIRTLITAIGTGVGESFDIENSRYGKIIIMTDAD
                     VDGAHIRTLLLTFFYRYMSPLLEQGMIYIARPPLYKVKRGRSEEYLYNERALQKYAEE
                     NKNQNFSIQRYKGLGEMNPDQLWDTTMDPDNRIIMQVQIEDAIMADETFTTLMGDKVK
                     PRREFIQEHAQEVKNLDI"
     gene            complement(4943..5194)
                     /locus_tag="VKN64_11910"
     CDS             complement(4943..5194)
                     /locus_tag="VKN64_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HMA60980.1"
                     /translation="MSVLFLHIGKGNMIKKEDVVFIGDIESTKDSKITEEFFDIIREE
                     GFIINNFDDEPRSFILTGEKIYLSIISSNTLRSRMEEII"
     gene            complement(5195..6313)
                     /gene="recF"
                     /locus_tag="VKN64_11915"
     CDS             complement(5195..6313)
                     /gene="recF"
                     /locus_tag="VKN64_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HMA60981.1"
                     /translation="MYIEKLLLKNYRNIDDTYLNLDPNLNIFIGNNGQGKTNLLEAVY
                     LMATTESHRTNRDNELIQWDKESALVQLKLIKSDYNLTISYKIEGRNKQIKINKNPLE
                     KISDLVGNLNVILFSPEDLKIVKGSPSNRRKFLDLEISQVNPYYYHLLKEYDHILRQR
                     NNLLKKMYNKSKRDIDLLEVWDDKLAENGAKIIDKRLKVLNKLKILARLSQRKLTSGQ
                     ENLNIKYDCTLNIDKNNDKNEINKIFLNKLKRDREDEFERGYTLSGPHRDDLILKINK
                     MNVRKYGSQGQQRTVALALKLAELEFMKSESGEYPVLLLDDVFSELDKNRRNTLLSII
                     NDKIQTLITTTDSEDIETLIENKKGNIFAVKDGVIQKR"
     gene            complement(6317..6532)
                     /locus_tag="VKN64_11920"
     CDS             complement(6317..6532)
                     /locus_tag="VKN64_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMA60982.1"
                     /translation="MEEIKIKTETIKLDQFLKWANIVSTGGEAKALIQSGGVRVNNKI
                     EKRRGLKLKDGDLVNVEGLDETLKVSG"
     gene            complement(6542..7666)
                     /gene="dnaN"
                     /locus_tag="VKN64_11925"
     CDS             complement(6542..7666)
                     /gene="dnaN"
                     /locus_tag="VKN64_11925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMA60983.1"
                     /translation="MNFKIEKKDFYQGLNITHRACSSSNTMPILKGILIEANKDKGLH
                     LMGTDLEIGIENWVPAEVEEEGKIVLPANQLRNIIRELPNEEIKFKADLEKFRADINC
                     LNSQFTINGYNPEEYPQLPDVSIPTELEIPAGKLKKIIDEVEFSTSTDQSQPVLTGAL
                     MVLKDEEIEMVSTNKYRLAYSKLENKNNIEEKLEIIIPGSTIKEISNLLEEEGTVEMK
                     VNENYASFDFNDIIVVSRLIEGKFPNFQQVIPDEKNTTITVNKEYLQSAVKRASLIAR
                     LDSNIVSLKSEDNRLVIESEDSDSGSAHEEVPIQLEGENHKISIDASYLLDVLKVVEV
                     EKVNLDLIGSLNPLVIKKEASDDYIYLIMPIRPGNEEEGE"
     gene            complement(7922..9319)
                     /gene="dnaA"
                     /locus_tag="VKN64_11930"
     CDS             complement(7922..9319)
                     /gene="dnaA"
                     /locus_tag="VKN64_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMA60984.1"
                     /translation="MAFSQEELEHIWNQTLNEVKDKLSNPSFKTWFSNTEPVDLKDDN
                     ILMVEVPNDFIKDWIETRYNELIGDILQDLTDTNLKCRFYTEEELQEINEVEEENQEE
                     PGPKEEEQKEKPEKKTREYNINNNLNPKYSFDSFVVGNGNRFAHAAALAVAEAPAKAY
                     NPLFLYGDVGLGKTHLMQAIAHYILKHNPDNKVVYVSSETFTNELINAIKDDKTASFR
                     DKYRNIDILLVDDIQFLANKERTQEEFFHTFNSLHEANRQLIISSDRPPKEIPTLEER
                     LRSRFEWGLITDIQKPDLETRIAILRKKADMENLKVPNEVIIYIANNIQSNIRELEGA
                     LIKVIAYSSLVDKEIDIDLAENALKDLIVENDEPREVNIDLIKDVVVDYYNLKEEDMT
                     SKKRTQKIAFPRQIAMYLSRELTDLSLPHIGEEFGGRDHTTVIHAYNKIEEKIEEDSE
                     FEKVVNKIISRIKNS"
     gene            9900..10226
                     /gene="rnpA"
                     /locus_tag="VKN64_11935"
     CDS             9900..10226
                     /gene="rnpA"
                     /locus_tag="VKN64_11935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMA60985.1"
                     /translation="METLKKKKDFDEVFKNGKSIASKYLVFYWNPNNKDKNRYGFSIS
                     KRIGKAVVRNKLKRRLKEIIRRDVESICNRGYDIIIIARNPVNDLDFHGIKKDLLRLF
                     KRAGLT"
     gene            10249..10458
                     /gene="yidD"
                     /locus_tag="VKN64_11940"
     CDS             10249..10458
                     /gene="yidD"
                     /locus_tag="VKN64_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMA60986.1"
                     /translation="MKQLVLWFIKVYQKVISPWTPKTCRFYPTCSEYSYQAVKKYGAL
                     KGGYKSIKRISKCHPFNPGGYDPLK"
     gene            10480..11130
                     /locus_tag="VKN64_11945"
     CDS             10480..11130
                     /locus_tag="VKN64_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HMA60987.1"
                     /translation="MFGWLINGIVDILKFLNGFVHNYGVTIILFTLIIKLVLYPLTAK
                     QTKSMKAMQDLQPEMDKIKKKHDDKEKQQQAMMDLYKEHNVNPAAGCLPMILQLAILI
                     PLYRAILSLSEVLNEEAFLWIGTLTSGSLAEPDVAIVMVNALAMIAQTYVTQKMQSGG
                     GGKSNMIMWIMPAFIIFIGFQLPSAILLYWFTSTVFTAAQQYIISNETVEEEVAEQ"
     gene            11130..11759
                     /gene="jag"
                     /locus_tag="VKN64_11950"
     CDS             11130..11759
                     /gene="jag"
                     /locus_tag="VKN64_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HMA60988.1"
                     /translation="MEKKRYKAESVNEAIKKGLDDLGIDRSQAEINIIEQGNKGFLGL
                     IGKKQAEIEIIMEDDPIKKGKNFLEEVFSKTNIDVEIDVVQEETNEEQVVYDIHSPDL
                     GIVIGRRGETLDALQYLTSLVVNKDTEEYYRIILDAEGYRDRRKKTLERLARKLAQKA
                     VNKERKVVLEPMPPHERRIIHITLKKDSRVRSYSEGEEPFRKVMIETVD"
     gene            11843..>12415
                     /locus_tag="VKN64_11955"
     CDS             11843..>12415
                     /locus_tag="VKN64_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMA60989.1"
                     /translation="MSNMKEDTIAAIATPYGTSGTGKVRVSGPEAIEIGAKIFKASSG
                     IDLNEADSHTAHHGYVVDPITKDTIDEVLAIIMVHPKSFTGENTVEFDCHGGMVPLES
                     VLELVLDQGARMAEPGEFSRRAFMNGKLDMAQAEAIIDVINSKTEKSLSIAMEQLKGG
                     LSEKVNDIKDDVITLLAHLEASIDFPEDEIE"
BASE COUNT         3740 a         2333 c         1914 g         4428 t
ORIGIN      
        1 actttctcgc tcgacttgca tgtgttaagc acgccgccag cgttcgtcct gagccaggat
       61 caaactctcc atttaataaa tgaaaagctc tttccatagc taaaaacttt gctcgctatt
      121 gaaagttctt acttgaaact tgttaagaac tgtcagggtc tgctgttcac ttttcaaggt
      181 tctaaaagtt tattaaaata taaaaaatcg cctgtcaaaa tcacgttctt taacagcgcg
      241 acctttaata tagtaacatt gtgaggggta tttgtcaaga gcaaatccaa agaaaatcat
      301 aataaataat aaaaaaggct aaatattgta ttatcaacta taataagaca tttcccggga
      361 aatattttac agcaaaaatc ccctccgact ataaaaatca aaggggatta ttttaaatta
      421 aattcttata ttactatatc ttatttattg atctaccttt tcttcatttt cctctttatc
      481 ttctttctct acttttttat tttcttcttt ttccttttcg acttcttcat ctgcttcttc
      541 ttctaagtct tctggtttaa tttttccaac agaaactaca taatcatctt cttctacatc
      601 aattacttta acacccattg tattacggcc tgtttttgaa atttcttcta ccctggttct
      661 aatgattata ccttctgccg taatgattat tatctcatct tctttattta ctaccttagt
      721 agaagcaatg gggccatttt tatctgtgat atttaaggtt atgatacctt taccatatct
      781 ctgttgaatt ctataatttt caacatctgt tctcttacca tagccttttt cactgataac
      841 taagacatca gaaccttctg aatccatacc cattcctact aaataatcat catctgctag
      901 atcaatacct ttaactccct gggcagctct accgaccgat ctgatatcct tttcgttaaa
      961 tcggatggct ttcccctggt gagtaactaa gattacattt tcgcctttat tggtgttttt
     1021 tacttccatt aattcatcat tttcctgtag ttttatacca ataattccgg tatagttact
     1081 gtcatactca actagaggtg tctttttaat catgcctttt ttagtgacca ttgtaagata
     1141 tttactttcc tcaaagtcac ttagaggtat tattgaactt actttttcat caccctccag
     1201 ctggagtaag ttcacaatcg cagttccccg cgactgacga ctggcctgcg ggatctgata
     1261 tccttttagt ctatatacca gacctttatt ggtaaagaac aggaagtaat tatgagtaga
     1321 agtaataaat acattttcta caaaatcttc ctctctggta gtagtaccaa tcataccttt
     1381 gccgccccta cgctggcttc tataggtatc aacaggcatt cttttaatat aaccctgatt
     1441 ggtcatggtc acaactacct gttcttcttc aattaagtct tctaaatcta aatcagtggt
     1501 ttggttaagg atcttagttc ttctctcatc accatatcta tctctaattt ctctgatctc
     1561 ttctttaata atttctagtt ttttctcttc actacctaaa atttctttat aatattttat
     1621 atcttccttg agatcagcat attcagcatc tattttatct ctctctaaag cagtcagcct
     1681 ctgtaatctc attcttaaaa ttgcattggc ctgatcttcg gatagttcat atttgttaat
     1741 caactgctga cgggctgtat caacatcatc cgcttctcta attaactcaa tcacattatc
     1801 aatatcatcc aaagcaacca tatagccctc taaaatatgg gccctgcttt ccgctttatc
     1861 taattcatac ttagttcttc ttgtgataat ttctttctga aaatccagat agtggtcaat
     1921 tatttctcta agattaagta cctttggctc accctctaca attgagagca ttataatact
     1981 aaaggtaacc tgcatctggg aatgtttaaa taattgattt agaacaattt taggattgga
     2041 gcctcttttg atcttaataa caatcctcat acctcttcta tcagattcat ctctaatatc
     2101 actgatgcca tctatctttt catttcttac catatcggca atcttttcaa ttaaacgggc
     2161 tttgttaact cgatagggca gttcatcaat aataatccga tctgccgatg tttcattttc
     2221 ttcaattctt gcttttgccc taactgttac cttaccgcgc ccggttttat aggccttata
     2281 gattgcccct ttacccatta taagtccacc ggtgggaaaa tcagggcctt taataacttc
     2341 cataagttct gctaaatcaa tatcgggatt atctattacc ctaacgatcc catcaataac
     2401 ttctcttaaa ttatggggtg gtatacttgt tgacatccca actgcaatac ccgaactacc
     2461 atttaccaat aaattaggaa aacgggacgg gataacatcc ggttctttta aggtatcatc
     2521 aaagtttggt ataaaatcaa cagtatcttt atttaaatct ctaagtagtt ctgtagttat
     2581 cttggccatg cgggcctcag tataacgcat ggctgcagct gagtccccat caacagaacc
     2641 aaagttaccg tggccatcaa ctaaaggata acgataagag aaatcctggg ccattctaac
     2701 cattgtatca tatacagccg tatcaccgtg gggatggtac ttacctaaga cctctcctac
     2761 gatacgggcc gattttttat gagatttatt gggggtcatt cctaaatcat gtagagcata
     2821 gagaatacgc ctgtggacag gtttaagccc atctctaaca tcaggcaggg ccctacttac
     2881 tataacgctc attgcataat ctaaaaaagc atccttcatc tggtcatcta tatcaacctg
     2941 tataacgttc tttccaccaa attcatcaac caatttttac accttccttt atctaaatat
     3001 caagattttt tacttcctgg gcatgttcct gaataaactc tcggcgtggt ttaaccttat
     3061 ctcccattaa ggtagtaaag gtttcatccg ccataatggc atcttcaatc tggacctgca
     3121 tgataattcg attatcagga tccattgttg tatcccagag ttggtcagga ttcatttctc
     3181 caaggccctt atacctctga atagagaagt tctgattttt attttcttct gcatattttt
     3241 ggagagctct ttcgttgtat aagtactctt ccgatctacc tctttttact ttgtataagg
     3301 gtggtcttgc tatatatatc ataccctgtt ctaataaagg actcatatac ctgtaaaaga
     3361 aggttaaaag taaggtccta atatgggcac catcgacatc agcatcggtc attattatta
     3421 tttttccata tctactgttt tcgatatcga aactttctcc cactccggtc ccaatcgctg
     3481 taattaaagt cctgatttca ttattattta atattttatt taatcttgcc ttttcaacgt
     3541 tgagtatttt tcctcttaaa ggtaaaattg cctggaagtt acgatctcta ccctgtttgg
     3601 cagagcctcc cgctgaatca ccctcaacta tatatagttc ggtgttttca gtatcacggg
     3661 aagaacagtc ggctaactta ccgggtaaag aagttgaaat caaggcattt tttcttctcg
     3721 ttaaatctct cgctttttta gctgccttcc tggccttagc agctttaata cccttttcta
     3781 cgatcgtctt agctacagaa gggttttctt ccataaaggc ttttagatgt ttagaaaggg
     3841 cagaatcaac aaaaccccgc acttcactat tgccaagttt ggttttggtc tgtccctcaa
     3901 actgaggttc ttcaatcttt atattaataa ctgcagtaag gccttctctt acatcttcac
     3961 cggttaaatt atcatctttt tcttttagta atcccttact acgggcatat ccattcaccg
     4021 ttctggttaa agcgctttta aagccgctaa ggtgggtacc accttctctg gtatttatat
     4081 tgttggcaaa tgtgaatatt ctttctttaa aaccactatg gtattgaaaa gatatttcta
     4141 gatccttatt ttcttcactt tcttctaaat agaccggatc tggaaaaagt ggtttcttat
     4201 ttttattcaa atatttaaca aactgaacaa ttccgccttc ataatggaat tcttcttctt
     4261 ttacttcttc ttcatctctt tcatctatta ctttaatatg gataccttta tttaaaaagg
     4321 ccatctcttt aaatctttgt aataatttat catacctaaa gttaatttct tcaaaaattt
     4381 ctggatctgg tttaaaagtt atcttcgtac cggtttctct ctcaacttca caggttccaa
     4441 tttgtttaag atcataaaca ggaacccctc ttctatattt ctgtctatat atattaccat
     4501 ctttccattt tacttcaact gtcatccatt ctgaaagagc attaactact gaaacaccga
     4561 ctccatgtaa accacccgat actttatatc cttcaccatc aaatttacca ccggcatgta
     4621 gggttgtcat tactacttca accgccggtt tgttaacttt aggatgggtt tcgatcggaa
     4681 tacctctccc attatcgaca acagttacaa tatttccctt ttttagatta acttcgatcc
     4741 tatcacagta accggccatc gcttcatcaa tactattatc tactacctca tatactaaat
     4801 gaagcaggcc atctatactg gtagaaccaa tatacatgcc gggtcttttt ctaactgctt
     4861 ccagaccttc taacacttgt atgttttcag cttcatatcg ccctttttta agttctgcca
     4921 tcatcatccc ccaattcttc tactaaatga tctcttccat tctacttctt agagtatttg
     4981 aagaaattat tgataagtat attttttcgc ctgttagaat aaatgatctc ggttcatcat
     5041 caaaattatt tataataaag ccttcttctc ttattatatc aaaaaactct tccgtaatct
     5101 tggaatcttt agttgattca atatctccaa taaaaactac atcttctttt tttatcatat
     5161 ttcctttccc aatatgtaaa aacaaaacac tcacctacct tttttggata accccatctt
     5221 ttacagcaaa gatattacct tttttatttt caattaaagt ttcaatatct tctgaatcag
     5281 ttgttgtaat cagagtctgt atcttatcat tgataatcga tagtaaggtg ttgcggcggt
     5341 ttttatccaa ttcagaaaag acatcatcta aaagtaagac cgggtattct ccactctcag
     5401 acttcataaa ctccaattca gctaacttca aagctaaagc tacggtcctt tgttgtccct
     5461 gagaaccata ctttctaacg ttcatcttat ttattttgag aatcagatca tctctatgag
     5521 gaccggaaag tgtatatccc ctctcaaatt catcttccct atctcttttt aatttattta
     5581 aaaatatttt attaatttca tttttatcat tatttttatc tatatttaag gtgcagtcat
     5641 acttaatatt taaattttcc tggcctgaag tcaactttcg ttgagacaat ctggccaaaa
     5701 tctttaattt atttaatacc tttaatcttt tatctataat ttttgcccca ttttccgcta
     5761 acttatcatc ccatacttct aaaagatcga tatcccgttt acttttatta tacatttttt
     5821 ttaataaatt atttctctgt cttaaaatat gatcatactc ttttaagagg tggtaataat
     5881 agggatttac ctgggaaatt tctaggtcta aaaattttct cctattactg ggactacctt
     5941 taacaatctt taaatcctct ggagaaaaca ggataacatt aagattacct accaaatcag
     6001 atattttctc cagaggattc ttattaattt ttatctgctt atttcttcct tcaattttgt
     6061 atgaaattgt tagattataa tcacttttaa ttaattttag ctgaactaac gcactttctt
     6121 tatcccactg gataagttca ttatctctat ttgttctatg tgattcagtt gtggccatca
     6181 gatagaccgc ttctaaaagg ttggtcttcc cctgaccatt atttccgata aatatattta
     6241 aattaggatc taagttaagg taagtatcat ctatattcct gtaatttttt aaaagtaact
     6301 tttctatata catttattat ccgctcactt ttaaagtttc atccaaccct tctacattga
     6361 ccaaatctcc gtcttttaat ttcaggcccc ttcttttttc aattttatta tttacgcgca
     6421 cgcccccgga ctgaatcaag gcttttgcct caccgcccgt tgaaacgata ttggcccatt
     6481 ttaagaattg gtctaactta atggtctcag ttttgatttt aatttcttcc atttatttaa
     6541 attactcccc ttcttcttca ttaccgggtc taatcggcat aattaagtaa atataatcat
     6601 cacttgcttc ttttttaata acaagtggat ttaaagaacc gatcaaatct aagtttacct
     6661 tttcaacttc aactactttt agaacatcta ataaatatga agcgtcaata cttattttat
     6721 ggttttctcc ttctaattga atcggaactt cttcatgagc actaccagaa tcagaatctt
     6781 ccgattctat taccaatcta ttatcttcac tttttagact tacaatattt gaatccagtc
     6841 ttgcaattaa agaagccctc ttaactgctg actgtaaata ctctttatta acagtaattg
     6901 tagtattttt ttcatcggga ataacctgtt ggaagttagg aaactttcct tctattaatc
     6961 ttgatactac aatgatatca ttaaagtcaa aacttgcata gttttcatta accttcatct
     7021 caactgttcc ttcttcttct aaaaggttac tgatctcttt tatagtggat ccgggtataa
     7081 ttatttctaa cttttcttca atattatttt tattttccaa tttactataa gctaaacggt
     7141 atttgttagt agaaaccatt tctatttctt catctttaag gaccattaaa gcccctgtta
     7201 aaactggttg gctttgatca gtagaagtag aaaactctac ttcatcgatt atttttttca
     7261 gtttaccggc cgggatttcc agttcagttg gaatactaac atccggtagc tgtggatact
     7321 cttccggatt atagccgtta atcgtaaatt gagagtttaa acagttaata tctgccctga
     7381 acttttctag atcagcttta aatttaatct cttcattggg tagttctctt ataatattcc
     7441 ttaactggtt ggctggtaat acgatttttc cttcttcttc tacttcggct ggtacccagt
     7501 tttcaatacc aatttctaaa tcagttccca tcaagtgcag gcctttatct ttgtttgctt
     7561 caattagtat accttttaag ataggcattg tattgcttga ggaacaagca cgatgtgtaa
     7621 tattcagacc ctgataaaaa tccttctttt caatcttaaa attcatcttc taacctccta
     7681 tgtaatagat agttattcac aaaaaaaata tagtaataat aatatatatt ttaatctaaa
     7741 ccagtagtag tagtagtagg gactgtgaat ttgtgtaaaa ggcgctctgt ggttgaatat
     7801 ttagtagttt aggggtgtag ataagatgta tataatagtt gagtggttat acacatacta
     7861 acatattaaa tctattaaca atcaatccac aggatattaa gaaaattata aacaggtact
     7921 attaggaatt tttgattcta gatattattt tattgactac cttttcaaat tcactgtctt
     7981 cctctatttt ttcttctatt ttattatagg catggataac agtggtatga tctctaccac
     8041 caaactcttc tccaatatga ggaagtgata aatctgttag ttctcttgat aagtacattg
     8101 caatctgtct cggaaaagca attttttggg ttcttttctt ggaagtcata tcctcttctt
     8161 tgagattata gtaatctacc accacatctt taattagatc aatattaact tcacgtggtt
     8221 catcgttttc tacaattaaa tctttaaggg cattttcagc taaatctata tctatttcct
     8281 tatctactaa agaagaatag gcaataacct taattaaggc accttctaat tctctgatat
     8341 tagattgaat attatttgct atatagatga ttacttcatt gggaactttc aagttttcca
     8401 tatcggcctt tttccttaaa atagcaatcc tggtctctaa gtccggtttt tgaatatcgg
     8461 tgataagccc ccattcaaag cgggatctta atctttcctc taaagtaggt atttctttgg
     8521 ggggacggtc actagaaatt attaactgac ggtttgcttc atgtaaggaa ttaaaggtat
     8581 ggaaaaactc ttcttgagtt ctttctttat ttgctaaaaa ttgaatatca tcgaccaata
     8641 ggatatctat atttctgtat ttatctcgaa aggaagctgt tttatcatct ttaatagcat
     8701 ttataagttc atttgtaaat gtttctgagg aaacatatac aaccttatta tcaggattat
     8761 gtttcaatat ataatgggcg atagcctgca ttaagtgggt tttacctaat cccacatctc
     8821 cgtataagaa aagaggatta taggctttag ccggtgcttc agcaaccgct agggcagctg
     8881 catgggcaaa tctattacca ttacctacta caaaggagtc aaaagaatac ttgggattaa
     8941 gattattatt tatattatat tcccttgttt tcttttcagg tttttctttt tgttcttctt
     9001 cttttggacc tggttcttct tggttttcct cttcaacctc attaatttct tgcagctctt
     9061 cttcagtata aaaacggcat tttaggttag tatcagttaa atcctgtaaa atgtcaccaa
     9121 taagttcatt ataacgggtt tcgatccagt ctttgataaa atcattgggt acttccacca
     9181 ttaatatatt gtcatctttt aaatctaccg gttctgtatt agaaaaccag gtcttaaaac
     9241 ttgggtttga tagtttatct tttacttcat ttaatgtttg attccagata tgttctaatt
     9301 cttcttggga aaacgccatt taaatacctc ctgagcaaaa tatgattgat acagcataat
     9361 tacgaaagtt atccccaaaa tatattaaaa tatgagagtt attaacatat ttgtggacaa
     9421 aagagcgagt tatccacaat atccagttgt ttataattac cttaaactag gtaaaaatgg
     9481 taattaggac aaaaatccct gttaaaaaat gaaataaaat ttccacataa cgttaataag
     9541 tttgattttt attaacagat tgtgaataac tgtggataaa aatgtggata aacaggggaa
     9601 ttgtggatgg gacagcactt ttatgtgaat aaattgatta acagggtgtg aaaatcttgt
     9661 aaaaacctct aattgaagtt ataatagtta ttaaagaaaa ttactatata tttaattatc
     9721 gttaatcatt aatattttaa cagattatca acaattattc aacaagaatt aatggctttg
     9781 aagttgtgga taaagataaa aattcttgac tctagatagc aaataacata taatttaaac
     9841 cgtatgagag ttgatattat aatatttttt tattaaacta atttgtagga ggaaattatt
     9901 tggaaaccct taagaagaaa aaagattttg acgaagtttt taaaaacggg aaatcgattg
     9961 cttctaagta tcttgttttc tactggaatc caaataataa ggataaaaat cgctacggct
    10021 tttctattag caaaaggatc gggaaagctg tggttaggaa taaattaaaa aggcgtttaa
    10081 aggagattat tcggagagat gtcgagagta tctgtaacag aggttatgat attattatta
    10141 tagcccgtaa tcccgttaat gatctggact ttcacgggat aaaaaaagat ctattaagac
    10201 tgtttaaaag ggctggtttg acttaaaaat ttagggaagt gaattaaatt gaaacaactg
    10261 gtattatggt ttattaaagt ttatcagaag gttatttctc cctggacgcc aaaaacctgc
    10321 agattttatc ctacttgttc tgagtactct tatcaagcag taaaaaaata cggtgcactt
    10381 aaaggtggat ataaaagcat taaaaggatt tctaaatgtc atccttttaa tccaggaggc
    10441 tatgatccac tcaaataatt atttaggagg aagtaaatta tgtttggttg gttgattaat
    10501 ggaatagttg atattttaaa atttttaaat ggttttgtac ataattatgg tgtgactatt
    10561 attttattta cacttataat taaattggta ttataccctt taactgcaaa gcaaactaag
    10621 tccatgaaag ctatgcagga cttacaacct gaaatggaca agatcaaaaa gaaacatgat
    10681 gataaggaga aacagcagca ggctatgatg gatttgtata aggaacataa tgtaaatcca
    10741 gccgcgggtt gtctcccgat gatcctacag cttgctattt taattccgtt atatagggca
    10801 attttgagtc ttagcgaggt tcttaatgag gaagcctttt tatggattgg aaccctaaca
    10861 agtggttcct tagcggaacc ggatgtcgca attgtcatgg ttaacgccct ggcaatgatc
    10921 gcccagactt atgttactca gaaaatgcag tctggcggtg gcggaaagag taatatgata
    10981 atgtggatta tgcccgcatt tattatattc atcggatttc agttaccttc cgcaatcttg
    11041 ctttactggt ttacctcaac agtatttact gcagcacaac aatatataat ttccaatgag
    11101 actgttgaag aggaggttgc tgaacagtaa tggaaaaaaa gagatataaa gctgaatcag
    11161 ttaatgaagc aataaaaaag ggacttgatg atttaggtat tgaccgttca caagcggaaa
    11221 taaatattat cgaacagggt aacaagggtt ttttaggcct gatcggtaaa aagcaggcag
    11281 aaatcgagat aataatggaa gatgatccga taaaaaaagg taaaaacttc ttagaagaag
    11341 tatttagtaa aacaaatatc gatgttgaga tcgatgttgt acaggaagaa accaatgagg
    11401 aacaggtggt gtatgatatc cattctcctg atctcgggat tgtaattggc cgtaggggag
    11461 aaaccctgga tgccttacag tatctgactt cattagttgt aaataaagat accgaagagt
    11521 actaccgaat tattcttgat gcagaaggtt atagggatag gcgtaagaaa acattagaaa
    11581 gattggcccg caaattagct caaaaggcag ttaataaaga aagaaaagtc gtgttagagc
    11641 caatgccgcc tcatgaaaga agaatcatcc atataacctt gaaaaaagat tctagagtaa
    11701 gatcatacag tgaaggtgag gaaccattta gaaaggttat gatcgaaaca gtagattaag
    11761 aataatatat aaaagtaatt agacatttaa tgcccagtag gtggtcctgc tgggtttaat
    11821 atttttgaag ggggttatta aaatgtctaa catgaaagaa gatacaattg ctgcaatagc
    11881 aactccctac ggtacttccg gcaccggtaa ggttagagtt agcggtccgg aggcaattga
    11941 aattggagct aaaatattta aagcatcttc gggtatagat ttgaacgaag ccgattctca
    12001 taccgcccac catggttatg tagtagatcc aatcactaaa gacaccattg atgaagtatt
    12061 ggccataata atggttcacc ccaaatcctt taccggagaa aatacggttg aatttgactg
    12121 ccacggcggt atggttccct tagaatcagt cctggaattg gtcttagatc agggagcgag
    12181 aatggccgaa ccgggcgaat tttcacgccg ggcttttatg aatggaaaac ttgacatggc
    12241 ccaggcggaa gctattattg atgtaataaa ttctaaaaca gaaaaaagcc tttcaatagc
    12301 gatggaacag ttaaaaggcg gactatctga aaaggttaat gatattaaag atgatgtcat
    12361 aacattatta gcccatctgg aagcttccat agattttcca gaagatgaga tcgag
//
LOCUS       DATGCJ010000270         7556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_486626, whole genome shotgun sequence.
ACCESSION   DATGCJ010000270 DATGCJ010000000
VERSION     DATGCJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486626"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VKN64_11960"
     CDS             complement(<1..693)
                     /locus_tag="VKN64_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636134.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HMA60990.1"
                     /translation="MRYYKYSKHLKKRYGVKTYKIPVNVKVSCPNRDGTLGREGCFYC
                     GETGAVFQNTDYEKSIVEQIESLKENIAEKYNAEKFIAYFQNFSNTYLPLEELKHHIK
                     EASSVQDVVEVCFSTRPDCISVRYLREITEMIEKNDLNINFTLELGLQTVNYHTLKDI
                     NRRHTLGEFIDAILTAHRYGIKVGVHLILNLPGDDMDDVIENAKVLSALKVDNVKLHA
                     LYIRENTQFAEMY"
     gene            complement(751..1362)
                     /locus_tag="VKN64_11965"
     CDS             complement(751..1362)
                     /locus_tag="VKN64_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60991.1"
                     /translation="MDDILYLESKEQLELIGDPFVLDILDALGYEVCTREEIIKTMNE
                     NPNMINDYLDNMIENNFLTVIEENVDKNKEKLRVTAKSIEGKGALFEHLDKKDLHWLR
                     GYINHLENRIVDLYSYLAEFEDPEAKLKELNYTNDLYASSNKVYLTAEEANELTNMIT
                     EFIKQNQKEIKEKSEDKEHNLYEFYAYMFPELKEFKKKVEKDN"
     gene            complement(1484..2002)
                     /locus_tag="VKN64_11970"
     CDS             complement(1484..2002)
                     /locus_tag="VKN64_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HMA60992.1"
                     /translation="MNTKDLVQASLLVAIGFILHAVFPPLLLGMKPDFSLAMMFIVLI
                     MKRDFKLGFLVALATGIFTALTTGFPGGQVANMVDKMVTFLIVFAMMGPILNRFNNKI
                     GVSVITLVGTLISGGVFLGTAALLFGLPGPFTVLFYTIVIPASLINAFTAVIIFSSIK
                     VGARVTSNYITD"
     misc_binding    complement(2195..2451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2539..3876)
                     /locus_tag="VKN64_11975"
     CDS             complement(2539..3876)
                     /locus_tag="VKN64_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60993.1"
                     /translation="MGFLNLLYQKFKYDRHLNFREEEIKKLQKEKLKKLVKYAYQNSA
                     FYKELFNNNQIRYEDLDQIQLKDLPITNKKDIMDNFDEVVTDKNISKKKIINFVDKNP
                     KPTSLMDNNYHIIHSSGTSGEIGYYIYNNKNWELLKAVGASRLFKSFGLQKKNYAFIG
                     ASDGHYAGISFFLSPVNRFEQYFYNDFLVMDINYPLHTYIDKLNELQPDVISGYPSAI
                     KMLLDYQKENELNIQPDNIICGGEPLTEEAYQKIKRYWQQEPVNYYGTSESIMMGVGF
                     GNEGLYIFDDLIILEFEDNKTYLTNLYNYTEPLIRYEMDDLILEGNIESKRWPFTFIK
                     NVSGRSEDLLWLENENQNLDFIHPIVFVELHIKGLKKFQVIKSDDLNIIFKAVPKEEI
                     NQDELRENILKKLNEILTKKNMENINLEIVIVDDIANNPKTGKYEIIKTRDNN"
     gene            3935..5278
                     /locus_tag="VKN64_11980"
     CDS             3935..5278
                     /locus_tag="VKN64_11980"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMA60994.1"
                     /translation="MFYYRFKKDKDRNCDIYLFTNKKIYEQIENDALKQLFDASKLPG
                     AVKVVGMPDIHIGYGLPIGGILAVDADQGIVSPGAVGFDINCGVRLLTTELNKKDIRN
                     DIKKILNSFKRNIPAGVGVDSDLNFSQKEFTNIVENGVPFLINAFNVGEQKDLEHIED
                     RGVFPNANMNNVSSKALKRGITQLGSLGSGNHFVELQYIDKVFADDTDLKEGQITVMI
                     HTGSRGFGHQIAKDYIDQAVKVNQKFDFTFPVKNLACFPIKSKEGHSYLSAMGCAANF
                     AYANRQLLTHKIRNIFAHLYPDSPLELFYDLTHNIARFEKHKIDDKKRSYLIHRKGAT
                     RLDFNSFALLPGSMGTGSYIIKSANSDSTSQSLTSIAHGSGRVMGRREAKRSISNQQH
                     QDAIKNVEVTSSSGDSLLDESPLVYKNIEDVVESMIETKLAYPVVRLKPLAVLKG"
     gene            5291..6022
                     /locus_tag="VKN64_11985"
     CDS             5291..6022
                     /locus_tag="VKN64_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HMA60995.1"
                     /translation="MNVGAFLDIDGTLYRGSLMIQHFKKLIKYEVLDPVVWHSQVKST
                     YDEWKRRHGYYEDYLEELADIYINELKGMKISDLDFVNDQVIRLSGDIVYRYTRCIVN
                     YHLQNNHKVFFISGSPEFLVEKMAAKYNVTDYRGSKYIMNQEGQFTGEVIKMWQSNNK
                     EKVLNEFVADYNIDLDKSYSYGDTQGDYSMLKMTGNPIAFNPNYDLITTIKKDPELKQ
                     KAEIVIERKDNIYKTDPDVEILDIE"
     gene            complement(6066..6545)
                     /locus_tag="VKN64_11990"
     CDS             complement(6066..6545)
                     /locus_tag="VKN64_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60996.1"
                     /translation="MSNEYDYVIVKIKINKLTKMLPKINHKREKEFLQKHMPRYLHFY
                     DYFLKKLFPFLSDFKENIAWKVIHNGMSFYVSKDRQQDGMFMSVAFQANNKLKFSFYY
                     EEDEEDQDLARESREGRNEDHMWYYVEELEFDNELAKLIVESYREKVKYLNQKDKEE"
     gene            complement(6636..6710)
                     /locus_tag="VKN64_11995"
     tRNA            complement(6636..6710)
                     /locus_tag="VKN64_11995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6676..6678),aa:Gly,seq:ccc)
     gene            complement(6877..7149)
                     /locus_tag="VKN64_12000"
     CDS             complement(6877..7149)
                     /locus_tag="VKN64_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMA60997.1"
                     /translation="MSIVEEFNLEQIEEGKTYELYEGSYSSDEEIERKLELAGYKFIA
                     EERRIYVTKVYADDMEKRIAVLDSGNIRIWEFESEEYYEMELEHHR"
     gene            complement(7243..>7556)
                     /locus_tag="VKN64_12005"
     CDS             complement(7243..>7556)
                     /locus_tag="VKN64_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMA60998.1"
                     /translation="KIIDIIQEGFDISKSVFIISDNSQEIKDEILANMRRGVTALRGY
                     GGYSGREKDVLLVTINRAEITKLKNLIRDIDEEAFVIMNEAHEVLGEGFKSVQERAIN
                     E"
BASE COUNT         2506 a         1368 c         1061 g         2621 t
ORIGIN      
        1 atacatttca gcaaattgag tattttctct tatatataaa gcatgtagtt taacattgtc
       61 aacttttaaa gcagagagca cttttgcgtt ttcaataaca tcatccatat catcaccagg
      121 taagttcaaa atcaagtgga ctccgacctt aattccatat ctatgggcag ttaaaatagc
      181 atcaataaat tcccccaggg tatgacgtcg atttatatct tttaaggtat ggtagttaac
      241 cgtctgcagg cccaattcaa gtgtaaaatt tatgtttaga tcattttttt caatcatctc
      301 ggttatttct cttaaatatc gaacacttat acagtccggc ctggttgaaa aacagacctc
      361 cactacatct tgtacagacg acgcctcttt tatatgatgt tttaattcct ctaaaggtaa
      421 ataggtatta ctaaagtttt gaaagtaggc aatgaacttt tcagcgttat atttttcagc
      481 aatattttct ttgagtgact caatctgttc cacaatactt ttctcataat ccgtgttttg
      541 aaaaactgcc ccggtttcac cacagtaaaa acagccttcc cgaccgaggg taccatcgcg
      601 gttaggacag ctcaccttta cattaaccgg tatcttatat gttttaactc catatctttt
      661 ttttaagtgt tttgaatatt tataatatct cataaaaatc acaaccccta aaattcagaa
      721 aagacagttt tacctgcctt ttcaaagaaa ttaattatct ttttcaactt ttttcttaaa
      781 ttctttcaat tcaggaaaca tataggcata aaattcatat aaattatgtt ctttatcttc
      841 acttttttct ttaatctctt tttgattttg tttgatgaat tcagtaatca tattagttaa
      901 ttcattagcc tcttcagctg tcaaatatac tttgttacta gatgcataaa gatcattagt
      961 atagtttaat tcttttaatt tggcttcggg gtcctcaaat tctgctagat aactatacaa
     1021 atctacgatt ctattttcta aatgattgat ataaccgcgc agccagtgta aatctttttt
     1081 atctaagtgc tcaaataaag cacctttacc ttcgattgac ttagctgtca ctcttaattt
     1141 ttctttgttt ttatcaacat tttcttcaat tactgttaaa aaattatttt caatcatatt
     1201 atcaagataa tcattaatca tatttgggtt ttcattcatt gttttaataa tttcttctct
     1261 cgtacatacc tcataaccaa gggcatccag tatatccagt acaaaaggat caccaatcaa
     1321 ctcaagctgc tctttactct ctaaatataa aatgtcatcc atactctcaa tacctccatt
     1381 taaattaaat aataaaatta aggctctaac ttataatatt ctataaagaa aaaatttttc
     1441 ctttaaaaaa ggatgggctt tttaagccca tccttctcgg aggttagtca gtaatgtaat
     1501 tggacgtgac tctagctccg acttttatgg aggaaaaaat tattacagct gtaaaagcat
     1561 taattaagct ggccggaata actattgtgt aaaacagaac tgtaaatggt ccaggcagtc
     1621 caaaaagtaa ggcagcagta cctaaaaata ctccgccgct aataagagtt cctactaaag
     1681 taattacact tacaccaatt ttgttattaa atctattcaa aatgggtccc atcatagcaa
     1741 atactatgag aaaagttacc atcttatcca ccatattagc tacctgtcca ccgggaaaac
     1801 cggttgttaa agcagtaaat ataccggttg ctaaagcgac caagaaacct aatttaaaat
     1861 cccttttcat aattaggact atgaacatca tcgccagaga aaagtcgggt ttcatcccta
     1921 acagtaaggg cggaaaaacc gcgtgtaaaa taaagccgat cgccaccaac aaagaagcct
     1981 gcactaaatc tttagtattc attaaattca tctccctttt aattttttat tattattcat
     2041 cttctactcc aactactccg ctttaaaaag agagatgtgc caccaggtat ccatattgat
     2101 ataattttta agtataaatt ttaaaataat cataacaatc atcctcacta cttgcctact
     2161 tttttattat aaaattaaaa aagtccttca ccccgaaagg gacgaaagac ttatattccg
     2221 tgttgccacc catgttgacc aaataaattt ggcccactca ttaggaaacc aaaagttgag
     2281 ttccttcttt tttaacggta agaaaccggc ttaggaatac tcttctaaaa aagaatttca
     2341 ccctgcatct cccaggtcca ttcactactg ctgttgatat cggattctca gccctaccga
     2401 ctctctgtaa tcaaacagtt aatagctact cgccctgttc atagacttta tgattaatta
     2461 ttcatttatt ttatataata ctagcacaat aattttaaac tgtcaaggat tttttgggat
     2521 ttagttaatc tgttattgtt aattattatc tctggtttta attatctcat acttgcctgt
     2581 ctttggatta ttggcaatat catccacaat aactatctct aaattaatat tttccatgtt
     2641 tttctttgtc aatatttcat ttagcttttt taaaatattc tctcttagct catcttgatt
     2701 aatctcctct tttggtactg ctttgaatat aatatttaaa tcatcacttt taatcacttg
     2761 aaacttcttt aaacccttga tatgtagttc aacaaatacg atagggtgga taaagtctaa
     2821 attttgattt tcattttcca gccacaacag atcttctgac cggccggaaa catttttgat
     2881 aaaagtaaag ggccatcttt tgctctctat atttccttct aatattaaat catccatttc
     2941 atatctaatt aatggttcgg tataattata taggtttgtt aaataagttt tattatcttc
     3001 aaactctaaa attatcaggt catcaaaaat atatagcccc tcattaccaa agccgacccc
     3061 catcattatt gattcggaag ttccgtaata attgacgggt tcctgctgcc agtatctttt
     3121 tattttttga taagcttctt ctgttaaggg ttctccccca cagataatat tgtcgggctg
     3181 tatattaagt tcgttttctt tttgataatc taataacatc ttgattgcag agggatatcc
     3241 acttattaca tcaggctgta attcatttaa tttatcaata taggtatgaa ggggataatt
     3301 tatatccatc actaaaaaat cattataaaa gtattgttcg aatctattga ccgggctcaa
     3361 aaagaaacta atccccgcat agtgaccatc ggaagcacct ataaaagcat aatttttctt
     3421 ctgtaaccca aagcttttaa ataagcgaga agcaccaact gctttaagta attcccaatt
     3481 tttattatta tagatataat aaccgatttc accggaagtg cctgaactgt gaataatatg
     3541 ataattatta tccattaaag aagtaggctt gggattctta tcaacaaagt taataatctt
     3601 ctttttactg atatttttat ctgttactac ctcatcaaaa ttatccataa tatccttttt
     3661 atttgtaata ggaagatctt ttagttgaat ctgatctaaa tcttcatatc ttatctggtt
     3721 attattaaat aattctttat aaaaagcact gttctgatag gcatatttaa caagtttttt
     3781 taatttttct ttttgtaatt ttttaatctc ttcttctcta aaatttaaat gcctatcgta
     3841 tttaaatttt tggtataata aatttaaaaa acccattttg ctcacctcat taacaaatat
     3901 ataaaaatct gatacaatag aagtggtgat atatatgttt tattatagat ttaaaaaaga
     3961 taaagacaga aattgcgata tttatctgtt taccaataaa aaaatatatg aacagatcga
     4021 aaacgatgcc ttaaaacagc tctttgatgc cagcaaatta cccggagcag tcaaggtagt
     4081 cggtatgccg gacattcata ttggctatgg cctgccgatc ggtggtatcc tggcagtcga
     4141 tgctgaccag ggtatagtat ccccgggtgc agtaggtttt gatataaact gtggagtaag
     4201 acttttaaca actgaactta ataaaaaaga tattagaaat gatattaaaa aaattcttaa
     4261 ttccttcaaa agaaatattc cggccggggt aggtgtagat tcagatctca attttagcca
     4321 aaaagaattt accaatattg ttgaaaatgg tgtgcccttt ttaattaatg cctttaatgt
     4381 cggtgaacaa aaggatttag aacatataga agataggggt gttttcccta acgctaatat
     4441 gaacaatgta tcttcaaagg ccttaaaaag aggaatcact caactgggca gcctaggttc
     4501 tggaaatcac tttgttgaac tacagtatat tgataaagtc tttgcagatg atacagacct
     4561 aaaagaaggt caaattacag tcatgatcca caccggttcc cggggctttg gtcaccaaat
     4621 cgccaaggat tatatcgatc aagctgtaaa agtgaaccaa aagttcgact ttacctttcc
     4681 ggtcaaaaat ctagcctgct ttcccataaa atcaaaagaa ggccactcat acctgtccgc
     4741 tatgggttgt gcagccaatt ttgcctatgc caaccgccag ctgttaactc ataaaattag
     4801 aaatattttt gcccacttat atcctgattc tcccttagaa ttattttatg acttaactca
     4861 taatatagca agatttgaaa aacataaaat agatgataaa aaaagaagtt atctaatcca
     4921 ccggaaaggg gctacaagat tagattttaa ttcattcgcc ttactgcccg gctcgatggg
     4981 gaccggcagt tatatcatca agtccgctaa ttcagattct accagtcaat ccttaacttc
     5041 aattgcccac ggttccggcc gagtaatggg caggagagaa gcaaaaagaa gtatcagtaa
     5101 ccaacagcat caagatgcta taaaaaatgt ggaggttacc tcttcttccg gtgacagttt
     5161 attagatgaa tctcccctgg tttacaaaaa tatcgaagat gtagtagaat caatgattga
     5221 aacaaaactg gcctatcctg tagtcagatt aaaaccattg gcagtcttaa aaggctaaag
     5281 gagtgatagc atgaatgtag gagcattttt agatatagat ggtactttat acagaggttc
     5341 ccttatgatt cagcacttta aaaaattgat caaatatgaa gttttagatc cagtagtctg
     5401 gcacagccag gtcaaaagta cttatgatga atggaaaaga agacatggct attatgaaga
     5461 ttatttagaa gaactagctg atatttatat aaatgaatta aaagggatga aaatatctga
     5521 tttagatttc gtaaatgatc aggtgattag attaagcggt gatattgtct atagatatac
     5581 ccgctgtata gtaaattatc atttacaaaa caaccacaag gtatttttta tttccggcag
     5641 ccctgaattt ttagtagaaa agatggcagc taaatataat gtaactgact accggggcag
     5701 taaatatata atgaaccaag aaggtcagtt tacgggtgaa gtaattaaaa tgtggcaatc
     5761 aaataataaa gaaaaagtac ttaatgaatt tgtggctgat tacaatatag atttagataa
     5821 aagttactcc tatggtgata ctcagggtga ttacagtatg ttaaaaatga cgggtaatcc
     5881 aattgctttt aatcccaatt atgacctaat aaccactata aaaaaggacc cggaactaaa
     5941 acaaaaagca gaaattgtca ttgaaagaaa agataatatc tataaaactg acccggatgt
     6001 agaaatatta gatattgaat aaaggttata aaaaaattaa aacctccttt taggaggttt
     6061 aatttttact cttctttatc tttttgatta agatatttta ctttttctct gtaactttcc
     6121 actatcaact tggcaagttc gttatcaaac tctaactctt ccacatagta ccacatatga
     6181 tcttcatttc ttccctctct agactcacgg gcaagatcct gatcttcttc atcctcttca
     6241 taataaaagg aaaatttcaa tttattattt gcttgaaaag caaccgacat aaacataccg
     6301 tcctgttgcc tatcttttga tacgtaaaaa ctcatgccat tatgaatgac tttccaggct
     6361 atattctctt tgaaatctga taaaaaagga aataacttct ttaaaaaata gtcataaaaa
     6421 tgtaggtacc taggcatatg tttttgcaaa aattctttct ctcgtttatg attgatcttg
     6481 gggagcatct ttgttaactt gtttattttt atctttacga tcacataatc gtattcattt
     6541 gacatgatat ccctccataa ttaaagatca acgaatattt catcttatta taacattata
     6601 taataataca ggtgaaaagt aaactttcat acaaatggag cgggtaacgg gacttgaacc
     6661 cgcaacttca accttgggaa ggttgcactc tacctttgac ttatacccgc ttattataat
     6721 tataacactt caatgatatt tattaaactt aacataggct gcttttttgt aattaattac
     6781 aaaaaatcta gatatctttg gtcaaatgtt atagttttct attttaaaga aaaaacagca
     6841 ataattttct ttatattgcg cttttcacat ttattattat ctgtgatgtt ctaattccat
     6901 ttcataatac tcttctgact caaactccca gattcggata ttgccagaat ctaaaactgc
     6961 aattcttttt tccatatcat cagcataaac cttagtaaca tatatacgac gttcttctgc
     7021 aataaattta tatccagcta gttctaattt tctttcgatt tcttcatctg aggagtaact
     7081 accttcataa agctcatagg ttttaccttc ttcaatttgt tctaaattaa actcttcaac
     7141 tattgacatc cgaaaaaccc ccttttagat atacaaacta ttatatacaa tatatattat
     7201 atcatatttt taaaatttat aaaaagtcac ttttaaatat tattattcat taatcgcacg
     7261 ctcctgtaca ctcttaaatc cttcacctaa tacttcatgg gcttcattca taattacaaa
     7321 ggcttcttca tcaatatctc ttattaaatt ttttaatttt gtaatttcag ctctattaat
     7381 tgtaactaaa agaacatctt tttctctacc gctataaccg ccatatcctc ttaaagctgt
     7441 tacccctcga cgcatattag ctaaaatctc atccttaatt tcttgagaat tatcagatat
     7501 tataaagaca gatttagaaa tgtcaaatcc ttcttgaatt atatctatta ttttag
//
LOCUS       DATGCJ010000271         4268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Halanaerobiales bacterium isolate SMAG_U9645
            k141_487400, whole genome shotgun sequence.
ACCESSION   DATGCJ010000271 DATGCJ010000000
VERSION     DATGCJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965216
            Sequence Read Archive: SRR5468419
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Halanaerobiales bacterium (soil metagenome)
  ORGANISM  Halanaerobiales bacterium
            Bacteria; Bacillota; Clostridia; Halanaerobiales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 09:41:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,407
            CDSs (total)                      :: 2,367
            Genes (coding)                    :: 2,362
            CDSs (with protein)               :: 2,362
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 2 of 5
            Pseudo Genes (incomplete)         :: 1 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Halanaerobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487400"
                     /isolate="SMAG_U9645"
                     /isolation_source="soil"
                     /db_xref="taxon:2094029"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="37.4958 N 122.1331 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VKN64_12010"
     CDS             complement(<1..280)
                     /locus_tag="VKN64_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HMA60999.1"
                     /translation="MTKSVDRLYAEIKKGNENSIARAISMVEDGTKNALELMKKVYNN
                     VDSAYMVGISGPPGSGKSTLTQRIVQLWREKDLKIGIIAIDPTSHFSGG"
     gene            complement(286..675)
                     /locus_tag="VKN64_12015"
     CDS             complement(286..675)
                     /locus_tag="VKN64_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HMA61000.1"
                     /translation="MDKKIKVLIAKPGLDGHDRGAKVIARGLKEAGMEVVYTGLRQTP
                     EQIVRSAVQEDVDVIGLSILSGAHTHLFPEIMKRLKEAGRDDIPVIAGGIIPEDDHQE
                     LRELGIKAVFEPGTPISKIVEFIEDEV"
     gene            complement(694..2361)
                     /locus_tag="VKN64_12020"
     CDS             complement(694..2361)
                     /locus_tag="VKN64_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMA61001.1"
                     /translation="MSDKDLEKLEQEWKENKFDKTVNRFGQRKEEFKSDSGRTIKPLY
                     TPLDTEDIDYEEKVNYPGQYPFTRGVYPTMYRSKLWTMRQYAGFGNAKETNERFRYLL
                     EQGQTGLSVAFDLPTQIGYDSNHPLARGEVGKVGVAIDTLADMEVLFDQIPLDEVSTS
                     MTINSPASILLAMYKVVAEKQGVSSEKLAGTIQNDILKEYIARGTYIYPPEPSLRIIT
                     DIFDYCSKEMPRWNTISISGYHIREAGSNAVQELAFTIADGIEYVQAAVDIGMDVDDF
                     APRLSFFFNAQMDLFEEIAKFRAARRLWAEIMEERFNAKKDKSKMLRFHTQTAGSALT
                     AQQPMNNIMRVTLQALSAVLGGTQSLHTNSYDEALGLPTEDSVKIALRTQQILAHETG
                     AADTIDPMAGSHYVEKMTDELVEEAREYIEKIDDLGGMAKAIESGYVQREIQESAYQT
                     QKKIENKEKVVVGVNEFTEEESKKAENLLKVDPEVEKQQIEGLSKVKEKRNDKQVKQT
                     LKAVREAAQSDKNLMYPIVEAVKAYATIGEISDVLREEFGEYEGQYI"
     gene            complement(2354..2899)
                     /locus_tag="VKN64_12025"
     CDS             complement(2354..2899)
                     /locus_tag="VKN64_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMA61002.1"
                     /translation="MDRFEVRLSGAGGQGMILAGIIIAEAALHEEGLNVTQTQSYGPE
                     SRGGASRAEVVLSKEVIDFPKVIKPDILVALTQEAYDKYNHDVKESGIIIVDDSIETD
                     EDKIKTYKMPIFEKAIKEVEMKLSANILVLGIIDKILDQIRTSSLQKAIKKRVPENTI
                     AVNMKAFAVGQELAKGGKIGE"
     gene            complement(2902..3723)
                     /locus_tag="VKN64_12030"
     CDS             complement(2902..3723)
                     /locus_tag="VKN64_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMA61003.1"
                     /translation="MSKYDIEEYFRQDKLPHIWCPGCGHGTIMANLARAIDKAELDPN
                     KVVVVSGIGCSGRASGYMNFDTLHTTHGRAIAFATGIKLANPELNVIVITGDGDGAAI
                     GGNHLIHGGRRNIDLTVVLMNNQIYGMTSGQFSPMTPTGADATTTPYGNIDQNFDLAD
                     LTKASGASYIARGTAYNTRQLIEIFKDGLLHKGFSFIEAISQCPVNYGRRNDMDGALE
                     MLEWQKESSVNIKKWDQLSEEEKKGKFPTGVIYQEEKREYTEMYQEIIDSVQEED"
     gene            complement(3724..>4268)
                     /locus_tag="VKN64_12035"
     CDS             complement(3724..>4268)
                     /locus_tag="VKN64_12035"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMA61004.1"
                     /translation="EVGPEDFNPYEETEDLIPPMPAYGEGYRYHTTGLFHDKTGFPRG
                     TNEDAKKLMDRLMAKIDNNMDIISLYDQDIRDDDEIVVLAYGSTMRSAINAVTRARQA
                     GIKAGWIKLKTLWPFPAEYIKDLSEDVDTIIVPELNRGQIIKEVKEHVFGNARVEGIN
                     KYDGTLIKPDEILNMIKEVK"
BASE COUNT         1090 a         1023 c          736 g         1419 t
ORIGIN      
        1 ccccaccact aaaatggctg gtgggatcaa tagcaattat accgatcttt aaatcttttt
       61 cacgccagag ctgaacaatc cgctgagtta aagtgctttt accggaacca ggtggaccag
      121 aaattcccac catgtatgca gaatcaacat tattatatac cttcttcatt aattctaatg
      181 cattttttgt gccatcttcc accattgaga tggcccgcgc aatagaattt tcattccctt
      241 ttttaatctc tgcataaagt ctatcaacac tttttgtcat ctgctttata cctcatcttc
      301 tataaattca acaatctttg agataggagt acctggttca aatacagctt ttataccaag
      361 ctcccttaat tcttgatgat catcttctgg aataatgccg cccgcaatta ccgggatatc
      421 atctctgccg gcctctttta atcttttcat aatttctggg aatagatggg tatgagcacc
      481 ggagaggata cttaatccta ttacatcaac atcttcttga acagcacttc tcacaatctg
      541 ttcaggagtc tgtcttaaac ctgtataaac aacttccatg cctgcttcct ttaggccccg
      601 ggcaattact ttagcccctc tatcatggcc gtctaagccc ggctttgcta ttaatacttt
      661 tatctttttg tccacgatta caggcctccc ctcttatata tattgtcctt catattcgcc
      721 aaattcttct ctcagtacat cactgatttc accgattgtt gcataggcct taaccgcctc
      781 tacaatagga tacatcagat ttttatcaga ttgggcagct tcacgaactg cctttaaggt
      841 ctgcttgact tgtttgtcat ttcttttttc cttaaccttg cttaaacctt caatctgttg
      901 tttttccact tcgggatcaa ctttaagtaa gttttctgcc tttttgcttt cttcttctgt
      961 aaattcatta actcctacta cgactttttc tttgttttcg attttctttt gggtttggta
     1021 ggcgctttcc tgaatctctc tctggacata gccagattct atcgccttgg ccattccacc
     1081 caaatcatca attttttcaa tatattctct ggcttcttca accagttcat cggtcatctt
     1141 ttccacataa tgggaaccgg ccatcggatc tatcgtatca gccgccccgg tttcatgggc
     1201 taaaatctgc tgggtcctta aagcaatttt aacagaatct tcagtcggca gccccaatgc
     1261 ttcatcataa gaattagtgt gcagcgactg tgttccccca agtacagctg agagggcctg
     1321 taaggttacc cgcataatat tgttcatcgg ttgttgggct gttagggccg atccggccgt
     1381 ttgggtatgg aaccttaaca tctttgattt gtccttttta gcgttaaatc tctcttccat
     1441 aatctcagcc cagagccgtc tggctgccct aaatttggct atctcttcaa ataggtccat
     1501 ctgggcattg aagaaaaagg aaagtcgggg ggcaaaatca tctacatcca taccaatatc
     1561 aacagcagcc tggacatatt caatgccatc tgctatcgta aaggctaatt cctgaaccgc
     1621 atttgagcca gcttccctga tatggtaacc gctaatacta atcgtattcc agcgcggcat
     1681 ctctttcgaa cagtaatcga aaatatctgt aatgattcta agtgatggtt cgggtggata
     1741 aatatatgta ccccgcgcaa tgtattcttt taagatatca ttctggatag tacctgccag
     1801 tttttctgaa cttacgccct gtttctcagc tacaacctta tacatggcta aaagtataga
     1861 ggccggtgag ttgattgtca ttgaagtact aacttcatct aaaggaatct gatcaaagag
     1921 aacttccatg tcggctaagg tgtcaatcgc aacccctact ttaccaactt cacctctagc
     1981 taaggggtga ttggaatcat aaccaatttg tgtcggtagg tcaaaagcaa cactcaatcc
     2041 ggtctgaccc tgttctaata ggtatctaaa tctttcattg gtttctttag cattgccaaa
     2101 gcccgcatac tgacgcattg tccatagttt actgcggtac atggtcggat aaacccctct
     2161 ggtaaagggg tactgaccgg gataatttac tttttcttca taatctatat cttcggtgtc
     2221 taatggggta tataatggtt taatggtcct gccagaatca gatttaaact cttcttttcg
     2281 ctgtccaaac ctgttgacag ttttatcaaa cttattttct ttccattctt gttctaattt
     2341 ttcaagatct ttatcactca ccaattttcc ctccttttgc aagttcttgt ccaactgcaa
     2401 aagccttcat attaactgca atcgtatttt caggcactct tttcttgata gccttttgta
     2461 aagatgaggt tctaatctga tctaaaattt tatctatgat gcctaatact aaaatgttag
     2521 cagacagttt catctctact tctttgatcg ccttctcaaa tatgggcatc ttatatgtct
     2581 ttatcttatc ttcatcggtc tcaatactgt catcaactat tatgataccg ctttctttca
     2641 catcgtggtt atatttatca taagcctcct gggttaaggc aactaaaata tcgggcttaa
     2701 ttaccttggg aaagtcaata acttccttgc ttaaaacaac ttcggctcgg ctggcgcctc
     2761 cccgtgattc aggtccatag gactgggtct gggtcacatt taagccttct tcatggagag
     2821 cggcttctgc aattatgata ccagccagta tcataccctg gccaccggcc ccactcagcc
     2881 ttacttcaaa tctatccata attagtcctc ctcctgaaca gaatctatta tttcctgata
     2941 catttcagta tattctcttt tctcttcctg atatattaca ccggttggga acttcccttt
     3001 cttctcttct tcagaaagtt ggtcccactt cttgatattc acagaggatt ccttctgcca
     3061 ttctagcatt tcaagtgccc catccatatc attacggcgt ccgtaattaa caggacattg
     3121 actgattgct tctataaagg aaaatccctt gtgtaaaagt ccgtccttaa agatctcaat
     3181 aagctgtctg gtgttatagg cggttccgcg tgcgatataa gaagcaccgg aagccttggt
     3241 taaatcggct aaatcgaagt tttgatctat attaccatag ggagttgtag tggcatcggc
     3301 tcccgtagga gtcatcggtg aaaactgacc acttgtcatc ccgtaaatct ggttattcat
     3361 aaggactaca gttaaatcaa tattccttct gccgccgtga attaaatggt tgccgccaat
     3421 cgcggccccg tctccatcac cggtaattac aattacatta agttcagggt tggccaactt
     3481 gataccggtt gcaaaggcaa tcgcccgacc gtgggtagtg tgtaaagtat caaagttcat
     3541 gtagcccgaa gcacgacctg aacagccgat tccggatact accacaactt tattagggtc
     3601 aagttcggcc ttatcaatag cccgcgctaa attagccata atcgttccgt gaccacagcc
     3661 cggacaccag atatgaggca gtttatcctg tctaaaatac tcttcaatat catacttgct
     3721 catctatttt acctccttaa tcatattaag gatctcatcg ggtttaatta aagtcccgtc
     3781 gtatttattt attccttcta ccctggcatt tccaaagaca tgttctttaa cttctttaat
     3841 aatctgacct ctatttaatt ccggtacaat tatcgtatcg acatcttcgc ttaaatcttt
     3901 aatatattca gccggaaatg gccataaagt tttcaattta atccagcccg ccttgatacc
     3961 tgcctgacgt gcacgggtca cagcattaat ggcagaacgc atggtagaac cataggctaa
     4021 gacaactatt tcatcgtcat ctctaatatc ctgatcataa agactgataa tatccatatt
     4081 gttatctatt ttagccatca atctgtccat taatttttta gcatcctcat ttgtaccgcg
     4141 gggaaaaccg gtcttatcat gaaataatcc ggtggtatgg tatctatacc cttctccata
     4201 ggcgggcatc ggaggtatta aatcttcagt ttcttcatag ggattgaaat cttcaggacc
     4261 tacttccg
//
